LOCUS       DAOMVO010000001        33681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_169_length_33681_cov_14.354726, whole genome shotgun sequence.
ACCESSION   DAOMVO010000001 DAOMVO010000000
VERSION     DAOMVO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 33681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33681
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169_length_33681_cov_14.354726"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PKV30_00005"
     CDS             complement(<1..1488)
                     /locus_tag="PKV30_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRL28634.1"
                     /translation="MADPILIAQHGDIQCFILPGLANRHGLITGATGTGKTVTLQKLA
                     ESFSQIGVPVFMSDVKGDLTGISQPGNIKGKMADVLKERGLPTPEPVACPVTLWDVFG
                     EMGHPVRATISDMGPLLLARMLDLNETQTGVLNLVFKIADDNGLLLLDLKDLRSMLTF
                     VGENAKQFTTQYGNVSAASVGAIQRGLLTLEGQGGDKFFGEPMLNLDDMMQTVSGQGV
                     VNILSADKLMQSPRLYATFLLWMLSDLYERLPEIGDPEKPKFAFFFDEAHLLFNDAPK
                     VLLERIELVVRLIRSKGVGVYFVTQNPIDVPDTVLAQLGNRVQHALRAFTARDQKAVK
                     ATAETMRGNPQLNIETAITELATGEALVSFLDEKGRPGITQRVYVIPPGSQIGPITDA
                     QRAELIKSSLVAGTYEEAVDRESAYEKLAERGAQAVKPGAVTVSGTGAASGDAATPAA
                     EDGGLVGDALKWGKEALFGRTGPRGGQYDGLVQNVIKGEMRRMGRE"
     gene            1561..2289
                     /locus_tag="PKV30_00010"
     CDS             1561..2289
                     /locus_tag="PKV30_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRL28635.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRQRIWTRVVREIMVAARTGGGDPT
                     ANPRLRLAIEKAKAANMPADTVKRNIDKATGNLEGVSYEEIRYEGYGIGGAAIIIDTM
                     TDNRVRTVAEVRHAFSKYGGNMGTEGSVAFQFKHVGQLIFAPGVSEDKVMEVALEAGA
                     DDVITDDEGAIEVLTDPAAFEAVKNALEAAGLTPELAEVTMRAENTIDLTGDDAAKMQ
                     KLLDVLEDLDDVQDVFHNANVEAV"
     gene            2286..2456
                     /locus_tag="PKV30_00015"
     CDS             2286..2456
                     /locus_tag="PKV30_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28636.1"
                     /translation="MRFAAWLTTLLLVGCGGGSEVSWCIGSGSGGVSAGYNATQCPPE
                     SPAKNSTSAVSY"
     gene            2520..3806
                     /gene="purD"
                     /locus_tag="PKV30_00020"
     CDS             2520..3806
                     /gene="purD"
                     /locus_tag="PKV30_00020"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRL28637.1"
                     /translation="MKILVIGSGGREHALAWKLSQSPKVQQVYVAPGNGGTALSPNLK
                     NVPLTDLAALRDWALAEKIALTVVGPEQPLAAGVVDDFRAHGLRIFGPTQAAAQLESS
                     KAFSKDFMARHGIPTAAYETFSDPAAAHAYVDRLGAPIVVKADGLAAGKGVVVAATKE
                     EAHAAIDDMLGGNTLGVAHNDGGARVVIEEFLQGEEASFIVLCDGKNVVAMASSQDHK
                     RLKDGDEGPNTGGMGAYSPAPVVTPDVHARAMRDVILPTIRGMDKDGIPFTGFLYAGL
                     MIDGDGKPKTLEFNTRMGDPETQPIMMRLKSDLVDVLMAATSGKLDEVELKWDRRVAL
                     GVVLAAGGYPLSPKKGDLISGLPKPTDEVVVFHAGTVAEGDQVKTAGGRVLCVTALAD
                     SVKQAQTKAYDAARTIHFDGMQYRRDIGHRAVKASH"
     gene            3808..4728
                     /gene="hemF"
                     /locus_tag="PKV30_00025"
     CDS             3808..4728
                     /gene="hemF"
                     /locus_tag="PKV30_00025"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRL28638.1"
                     /translation="MAAIEPRAVRDYLIGLQARITHAIESRDGQATFLTDAWAKAPGE
                     PLQGDGITQILEGGAVFERAGCGFSHVRGPKLPPSATQHRPELAGAPFEAMGVSLVFH
                     PLNPYVPTVHMNVRMIAAGHPGAAPVCWFGGGMDLTPYYGFDEDCTDFHRVCRDALKP
                     FGDDKYPRFKQWCDEYFFNKHRSEPRGIGGIFFDDFSEGDAAHGFALMRSVGDAFLDA
                     YVPIVQRRVGMPWGERAREHQLIRRGRYVEFNLVWDRGTHFGLQSGGRTESILLSMPP
                     QVAWAYQRQPLPGTPEAALTERYLQAREWV"
     gene            4725..5354
                     /gene="nadD"
                     /locus_tag="PKV30_00030"
     CDS             4725..5354
                     /gene="nadD"
                     /locus_tag="PKV30_00030"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRL28639.1"
                     /translation="MSAAPLRIGLFGGAFDPPHDAHVALALAAINQLALDRLYVVPTG
                     DAWHKSRPLSPGAGRLAMCRLAFAHIPQVAVDDRELRRDGPTYTIDTLHELRAEHPGA
                     ELFLQIGADQAAAFHTWRRADEILQIATLSIAVRAYDSSAGDVFDPKNPLPGLATGGA
                     QVRILSLPAMTHSATDVRHRVAEGLPIDHLVPPAVAGYIAEHHLYQEPR"
     gene            5351..6115
                     /gene="rsfS"
                     /locus_tag="PKV30_00035"
     CDS             5351..6115
                     /gene="rsfS"
                     /locus_tag="PKV30_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRL28640.1"
                     /translation="MSETAAKKDVQKLQRAIVDGLEDVKAQDIRVFNTEHLSPLFERV
                     MVASGTSNRQTKALATSVRDKVRDAGFAKPRIEGEENGEWIIVDCGVAVVHIMQPTIR
                     QYYNLEEIWGEKPVRLKGPASKAAVNRRAATAPSKEKAAPAPAKKAPAATKKAAPAKK
                     PATKVAAKSPARKAPAKSPARKSAAPRGARGSASSTLQTVVVNKPSARAAAKAAAAQP
                     MGRAPAKAPAKKAPARAAPKGVAKPAARKTAPRTSR"
     gene            6125..6595
                     /gene="rlmH"
                     /locus_tag="PKV30_00040"
     CDS             6125..6595
                     /gene="rlmH"
                     /locus_tag="PKV30_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRL28641.1"
                     /translation="MKLLVIAVGQRMPDWAQTAWADYAKRFPPELRLELKAIKTEPRS
                     GGKTTEQIMTAERQRIEAAIPSGTHVVALDERGSALTTAALAAKLSQWQGQGGDVALL
                     IGGPDGLHPALRQAAHERIRLSDLTLPHAMVRVLLVEQLYRAWSINAGHPYHRD"
     gene            6633..7232
                     /locus_tag="PKV30_00045"
     CDS             6633..7232
                     /locus_tag="PKV30_00045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRL28642.1"
                     /translation="MVDFIYLASQSPRRRQLLEQLGVRLSLLLPDDSDAAEALEAVRT
                     GEAPATYVRRVTRLKLDDAVRRLAEQARPAAPVLCADTTVALGRAILGKPRDAQDAAD
                     MLRQLSGDTHRVLTAVAVQQGDWRGEAMSESRVRFATLRDDDIKAYVASGEWQGKAGA
                     YAIQGRAAAFIEHISGSYSGIMGLPLFETAALLRAAHAN"
     gene            7252..8742
                     /gene="rng"
                     /locus_tag="PKV30_00050"
     CDS             7252..8742
                     /gene="rng"
                     /locus_tag="PKV30_00050"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HRL28643.1"
                     /translation="MQQEILINWSPQETRVAVIEGGAVQELHVERTLERGLVGNIYLG
                     KVARVLPGMQSAFIDIGLERAAFLHVGDLWQKPLEGESLPAHRSGGPLKPIEKQVFEG
                     QTLMVQVIKDPIGTKGARLATQISIAGRMLVFLPQDDHIGVSQKIPAAQREQLRTRVQ
                     TLAGEAADGGKAGGFILRTNAEDASDAELAEDIAYLRKAWSRIREAAARQPPASLLHE
                     DLSLLQRVLRDLTGEHTQSIRIDSSEQFHALAAFGREFMPAAAAHLQHYKGERPIFDL
                     HSVDEEITKALGRRVDLKSGGYLIIDQTEALTTIDVNTGGFVGARNFDDTVFKTNLEA
                     AQAIARQLRLRNLGGIVITDFIDMTQPEHQSAVLAEFRKQLARDRVKTQVGGFSSLGL
                     VEMTRKRTRESLAQLLCEPCEQCQGAGRVRTARTVGYDILREILREARQFNPKEFRVV
                     AAPVVIDLLLDEESTHLAGLSDFIGKPISLQAESALGPDQYDIVLL"
     gene            complement(8790..10586)
                     /gene="msbA"
                     /locus_tag="PKV30_00055"
     CDS             complement(8790..10586)
                     /gene="msbA"
                     /locus_tag="PKV30_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HRL28644.1"
                     /translation="MNDARIQPPASPAAQRPWGARLARLWTYFSEYRLGWVLAIAGTL
                     ISALTEASVPALLKPLLDEGFTQGSLPIWTVPLAIVGLFALRGVAHFASQYALARIAN
                     EGMVKLRRQLFDRLLNADLALFARQSASQLANTIVYEVQTGTTMLVQSLLNLGRDSFS
                     VLALMGYLLYLNWSLTLIVLVLVPIVALIMKTMSRRLYRYTRLSQSTTDELAYVVEEN
                     VLAHRMVRLHGAQQQQAERFHTLSRALRGVSIKATSASAAAMPLAQMAAAVALSVVIA
                     IALQQGQASGGRGMSVGGFVSFIGAMLMLIQPLRRLTDVVGPVTRGLAALERGLDLMD
                     QVQPEVGPPASATPPARARGELALRDVTVIYANGAEPSLNALSLDVGAGETVALVGPS
                     GSGKTTLVNLLPRFVRPDSGQILLDGRDIADWPLTALRAQFALVSQDVTMLNDTVAAN
                     VALGAASIDRERVQRCLQDANLAAHVASLPQGIDTVLGHNATQLSGGQRQRLAIARAL
                     YKDAPVLLLDEATSALDTESERLVQQAITRAMAGRTTLVIAHRLSTVEHADRIVVMER
                     GRIVEQGSHAQLMAAGGLYARLQSHPGELSNE"
     gene            10772..12073
                     /gene="tolB"
                     /locus_tag="PKV30_00060"
     CDS             10772..12073
                     /gene="tolB"
                     /locus_tag="PKV30_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRL28645.1"
                     /translation="MNTDPPTSNPHPNWQRRGLVAALASAPLSAAWAQFRVEVSGVGL
                     TQLPIALVPFRGEDAAPQKISAIIRADLERSGQFRSVDASGEAMDETTRPDMSVWRQR
                     TADALAGGSVTRLPDGRYDVRFRLWDVVKGQDLGGQGYVVPQADLRLAAHKIADYIYE
                     KLTGEKGVFSTRLAYVTKAGSRYTLWVADSDGENAQAALGSPEPIISPTWSPTGAQLA
                     YVSFESRKPVIYVHDVSTGRRRLLANFRGSNSAPAWSPDGRNLAVTLSRDGGSQIYLI
                     SASGGEPRRLTQSQSIDTEPVFSPDGASIYFVSDRGGAPQIYRMPASGGNAQRVTFNG
                     SYNVSPTVSPDGRYLAYVSRTGGYKLQVMELASGNVTAVTDTAEDENPSFAPNSRLIV
                     YATRHGGREALMTTTVDGKIKARLAGRSGDIREPSWGPFQK"
     gene            12132..12671
                     /gene="pal"
                     /locus_tag="PKV30_00065"
     CDS             12132..12671
                     /gene="pal"
                     /locus_tag="PKV30_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRL28646.1"
                     /translation="MMKRYLMVAALAAVLAGCSSGVKLDPPIQGSGTTGADASATTGA
                     TGQGAGQSTVTQVQAGSVGADAAGPAGVGRVIYFDFDSFSVKPEYQSLVDQHARFLQG
                     NRGRSVAVEGHTDERGSREYNLALGQKRAEAVRRALTLVGATDAQIEAVSFGEEKPAA
                     SGASEDAYSQNRRVEIRYR"
     gene            12682..13473
                     /gene="ybgF"
                     /locus_tag="PKV30_00070"
     CDS             12682..13473
                     /gene="ybgF"
                     /locus_tag="PKV30_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRL28647.1"
                     /translation="MRMTYLRIAVLSLACVAGQSAQAQLFGDDQARQAILDLRQRFDQ
                     SVAAQNRLVDENTQMRRSMLDLQQQIESLRGELARSRGQEEQLTRDLSELRKLQTSQQ
                     SLEDRLRKLESAESARLATEAAASSTQSPPLPSDSAGASEKQDYDAALAVFRAGDFKG
                     SQTGFANFIKRYPRSNLAPSALFWLGNAQYATRDYKEAITNFRSLLTASPNHQRAPEA
                     MLSIANCQIELKDTRAARATLESLVKTYPQSEAAQAGRERLSRLK"
     gene            13575..13940
                     /gene="rpsF"
                     /locus_tag="PKV30_00075"
     CDS             13575..13940
                     /gene="rpsF"
                     /locus_tag="PKV30_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRL28648.1"
                     /translation="MRHYEIILLIHPDQSEQVPAMLERYKGMITAGGGKIHRQEDWGR
                     RQLAYQINKLGKAHYLCLNIEADQAVMAELEHAFKFNDAVLRHLTVQRKKADTAPSSM
                     MKTVEREEARKAQQAETTN"
     gene            13969..14256
                     /gene="priB"
                     /locus_tag="PKV30_00080"
     CDS             13969..14256
                     /gene="priB"
                     /locus_tag="PKV30_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HRL28649.1"
                     /translation="MNQTRLTASIVEIGAVRLTPAGVPALDARLEHESEVEEGGSSRQ
                     VKALLKAVALGAVAERLGRQPIGSAWCFTGFLASPRNAKSVVLHIQDFQQD"
     gene            14272..14553
                     /gene="rpsR"
                     /locus_tag="PKV30_00085"
     CDS             14272..14553
                     /gene="rpsR"
                     /locus_tag="PKV30_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRL28650.1"
                     /translation="MATFKKFNKDKRPKRNTQSLLFKRKRFCRFTVAGVEEIDYKDVD
                     TLRDFIGENGKIVPARLTGTRAFYQRQLNTAIKRARFLAMLPYSDQHRV"
     gene            14570..15022
                     /gene="rplI"
                     /locus_tag="PKV30_00090"
     CDS             14570..15022
                     /gene="rplI"
                     /locus_tag="PKV30_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRL28651.1"
                     /translation="MQIILLDKVVNLGNLGEIVKVKDGYARNFLIPSGRARRATEAAK
                     AEFEARRAELEKQAAEKLAAAQAQGEKLNGQTIKLTQKAGVDGRLFGSVTNHDIADEL
                     KKHGFDVHKSQVRLPNGPIKVVGDNTVQVSLHTDVDAEITVAVYGETA"
     gene            15206..16621
                     /gene="dnaB"
                     /locus_tag="PKV30_00095"
     CDS             15206..16621
                     /gene="dnaB"
                     /locus_tag="PKV30_00095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRL28652.1"
                     /translation="MSSVFPSSYADLADDAPTDRQVAQLRIPPHSLEAESSVLGGLLL
                     DNSAWDRVGDVLVDSDFYRYEHRLVYSAVGKLVNETKPADVITVYEELQRQGKGDEIG
                     GLVYLNALAQYVPSASNIRRYAEIVRERSILRKLVSASDEIATSAFNPKGRAVDKILD
                     EAEGKIFKIGEEGSRMKQGFQAMDGLVVQLLDRVQEMADNPNDVTGVPTGFVDLDRMT
                     AGLQAGDLVVLAARPSMGKTAFAINIAEHVALNEQLPVAVFSMEMGAAQLAVRIVGSI
                     GRIDQGHLRTGKLTDEEWPRLTEAIERLRNVSLHIDETPGLTPSELRANARRLARQCG
                     KLGLIVVDYLQLMSGSSSDGENRATELGEISRGLKALAKELQCPVIALSQLNRSVETR
                     TDKRPMMSDLRESGAIEQDADIIMFIYRDDYYNKDSREPGVAEIIIGKQRNGPTGTVK
                     LAFVKPLTRFESLALGASDDF"
     gene            complement(16733..17941)
                     /locus_tag="PKV30_00100"
     CDS             complement(16733..17941)
                     /locus_tag="PKV30_00100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HRL28653.1"
                     /translation="MSLFSAVEMAPRDPILGLNEQFAADQNPAKVNLGVGVYFDENGK
                     LPLLACVQAAEKALMDTPKPRGYLPIDGIAAYDNGVKALVFGADSDVVKQGRVATVQA
                     LGGTGGLKIGADFLKKLNPNAKVLISDPSWENHRALFTQAGFDVGTYRYYDAATRRLN
                     FDGMLADLKAAAPGTVVVLHACCHNPTGYDLTPDQWAQVIEVVKAGKLTAFLDMAYQG
                     FADGITEDGAVVGKFVAAGLDVFVSTSFSKSFSLYGERVGALSVVCASKEEADKVLSQ
                     LKIMIRTNYSNPPTHGGAVVAMVLGDAKLRAQWEEELAGMRKRIKAMRQALVDGLKAA
                     GVKQDMSFITTQVGMFSYSGLSKDQMVRLRDEFGVYGTDTGRMCVAALNEKNIGYVCE
                     SIAKVMQALR"
     gene            18064..20172
                     /gene="uvrB"
                     /locus_tag="PKV30_00105"
     CDS             18064..20172
                     /gene="uvrB"
                     /locus_tag="PKV30_00105"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRL28654.1"
                     /translation="MTTIAAADAPTSGQFQSFPDSPFQLYQPYPPAGDQPTAIDQLVE
                     GVENGESFQTLLGVTGSGKTFTMANVIARLGRPAIVFAPNKTLAAQLYSEFREFFPKN
                     AVEYFVSYYDYYQPEAYVPHRDLFIEKDSAINEHIEQMRLSATKSVLERRDVVIVATV
                     SAIYGIGAPDDYLQMRMILKVGGKQGQRDAIAQLVRMQYQRNDTDFSRGTFRVRGDTI
                     DVFPAEHSELAVRMELFDDEVESISLFDPLTGRIQQKVPRFVIYPSSHYVTPREKTLA
                     AVETIKQELNDRLKFFVGEGKLVEAQRLEQRTRFDLEMLAELGHCKGIENYTRHLSGA
                     APGDPPATLTDYLPKDALMFLDESHQMIGQLNAMYNGDKARKTTLVEYGFRLPSALDN
                     RPLKFDEFERRMRQVVFVSATPADYEKTHAGNVVEQVVRPTGLIDPEVEVRPATHQVD
                     DVLQEIRLRSDKRERVLITTLTKRMAEQLTEYLTDNGVKVRYLHSDIETVERVEIIRD
                     LRLGAFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAARNVNGK
                     AILYADRVTESMKKAIGETERRRAKQIEFNTVHGITPRGIVKQVRDLIDGVYSDKGGG
                     TDSQVLAAEMARARVEDMSEKDVSREIKRLEKLMLEHARNLEFEQAARVRDQLHVLKQ
                     SAFGAPGADNLRSIAGRSAA"
     gene            complement(20204..20728)
                     /locus_tag="PKV30_00110"
     CDS             complement(20204..20728)
                     /locus_tag="PKV30_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRL28655.1"
                     /translation="MTKSRTTRPAALALAVLMAAALGACSKSEQDQAARQVDQAAAKT
                     AEAGREMKADVQEAGRDAAAAARDATADAKQAGQELKADAASATSEIRAEASQAGAAV
                     KAAVDDAAITASISAKLAKDADLSALRIDVDTKDGTVTLSGPAPTQAAKDRASQLARE
                     VEGVKNVTNNLKVG"
     gene            complement(20769..21239)
                     /locus_tag="PKV30_00115"
     CDS             complement(20769..21239)
                     /locus_tag="PKV30_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRL28656.1"
                     /translation="MNTLYTRLLHTLPALLLAGGLAACARDDQSASNVMTAGSFDTAV
                     SRSQAAARDAERTAAEAEAEAKEAREAASAATSGARAPAPAADKDELISAAVRARLQR
                     QPALRGQPIQLYTAGGVVWLRGSVASDAAKEQAEQAASATDGVVAVRNRLDVRH"
     gene            21529..22062
                     /gene="iscR"
                     /locus_tag="PKV30_00120"
     CDS             21529..22062
                     /gene="iscR"
                     /locus_tag="PKV30_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HRL28657.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQDNGPVTLAAISQRQHISLSYLEQ
                     LFGKLRRHELVESTRGPGGGYSLGRKASEITVADIITSVDEPLDATHCGGKENCYGDG
                     GRCMTHDLWTSLNARMLEFLESVTLQKLVDDQHANGIEIEDKPATKRAISSAPVVKPI
                     RINAPNSVFALGGITTK"
     gene            22145..23365
                     /locus_tag="PKV30_00125"
     CDS             22145..23365
                     /locus_tag="PKV30_00125"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRL28658.1"
                     /translation="MSQTPHFPIYMDYGATTPVDPRVVDAMVPWLYEHFGNAASRSHA
                     WGWEAEKAIENAREQVADLIGADPREIVWTSGATESTNLALKGAANFYKGKGKHLITL
                     KTEHKATLDTMRELERQGFEVTYMEVQEDGLLDLEKFKAAIRPDTILASVLFVNNEIG
                     VIQDIAAIGAICREKGVLLHVDGAQATGRVNIDLKTLPVDLMSLTAHKTYGPKGVGAL
                     YVRRKPRVRLEAQMHGGGHERGMRSGTLPTHQIVGMGEAFAIAKKQMHDDNAHAARLQ
                     KRLLDGLKDVDQVFINGSLDKRVPQNLNMSFNFVEGESLIMGIKGLAVSSGSACTSAS
                     LEPSYVLRALGRSDELAHSSLRMTIGRFTTEEEIDYAIKTIRENVAKLRELSPLWEMH
                     QEGIDLSTIQWAAH"
     gene            23443..23829
                     /gene="iscU"
                     /locus_tag="PKV30_00130"
     CDS             23443..23829
                     /gene="iscU"
                     /locus_tag="PKV30_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRL28659.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDKGDDSVGTGMVGAPACGDVMKLQ
                     IKVNPQTGVIEDARFKTYGCGSAIASSSLVTEWVKGKTVDEAATLKNSQIAEELALPP
                     VKIHCSILAEDAIKAAVDDYRKKHAA"
     gene            23836..24159
                     /locus_tag="PKV30_00135"
     CDS             23836..24159
                     /locus_tag="PKV30_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28660.1"
                     /translation="MALQHASSGDVIDVRPYGARIDGAQSIALFKSTQLEVMRLVLPA
                     GHRMPPHHVPGEITIQCLEGVVRVDVAAASHDLPAGHLMYVAGGVEHALTAIESASVL
                     VTIAL"
     gene            24187..24510
                     /gene="iscA"
                     /locus_tag="PKV30_00140"
     CDS             24187..24510
                     /gene="iscA"
                     /locus_tag="PKV30_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HRL28661.1"
                     /translation="MAVSLTEAAARHVTKYLAKRGKGVGVRLGVKTTGCSGLAYKLEY
                     VDEVAPEDELFEQHGVKLMIDPKSLAYIDGTELDYVREGLNEGFRFNNPNERDRCGCG
                     ESFRV"
     gene            24510..24746
                     /locus_tag="PKV30_00145"
     CDS             24510..24746
                     /locus_tag="PKV30_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28662.1"
                     /translation="MAPGQLGDLAHARVGVTIDNEREVRALNAWFQRWGPRIRRMDDQ
                     GCGCCLDAWDVQAPPEALAELPRGMVRPALHAAA"
     gene            24757..25278
                     /gene="hscB"
                     /locus_tag="PKV30_00150"
     CDS             24757..25278
                     /gene="hscB"
                     /locus_tag="PKV30_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRL28663.1"
                     /translation="MNLQSTDFELFDVPARFAQDRAQLDARWKQLQREVHPDRFAAQG
                     AAAQRVAMQWSARINEAYQRLKYPQRRAAYLCELHGAPIQAENNTAMPAEFLMQQMEL
                     REQMDDAASTKDLDEISRSCLSAQRELLQKIEHQMDVEHDWPAAAQSVRALMFLDKLA
                     ADIERRHETLESN"
     gene            25329..27188
                     /gene="hscA"
                     /locus_tag="PKV30_00155"
     CDS             25329..27188
                     /gene="hscA"
                     /locus_tag="PKV30_00155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRL28664.1"
                     /translation="MALLQISEPGQTPDPHQRRIAVGIDLGTTHSLVAAVRHGVAECL
                     PDEQGRVLLPSVVRYLAGGGRQIGHDALIDAVADAENTIASAKRLMGRRLGDIVGRDQ
                     LPYRLLDQPGMVAIQTAHGEKSPVEVSAEILATLRYRAEDTFNDDLYGAVITVPAYFD
                     DAQRQATKDAAKLAGIHVLRLINEPTAAAIAYGLDNASEGVYAVFDLGGGTFDVSILR
                     LTQGVFEVIATGGDSALGGDDYDAALADWVLARTGATAATPADKAALKAAARRAKEAL
                     SASDNVAFSARLSSTEARFDLKPEDLEAATAALTARCMTTVRRVLRDAKLAPDEIKGV
                     VMVGGSTRMPVIRRAVAELFGREPLTNLNPDEVVALGAAIQANQLAGNDSAGDLLLLD
                     VIPLSLGLETMGGLVERIVPRNQTIPVAMAQDFTTYKDGQTALALHVVQGERDLVQDC
                     RSLARFELRGIPPMAAGAARIRVTFTVDADGLLNVSAREQISGVEAQIDVKPSYGLSD
                     EQIAQMLQDSFSTAEADVKARALVEAKVDADRMLLATQSALAADAALLAESERAEIDA
                     LMLSLRGIAANSVDAAAIEAATKALADGTEAFAAARMNAGIQRALAGKSLESI"
     gene            27192..27530
                     /gene="fdx"
                     /locus_tag="PKV30_00160"
     CDS             27192..27530
                     /gene="fdx"
                     /locus_tag="PKV30_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HRL28665.1"
                     /translation="MPTIKVLPHPEYAPEGAEIQANLGDSICEALLDNGIEIEHACEM
                     SRACTTCHCIVRKGFDSLQEAEEEEEDLLDRAWGLEPQSRLSCQAIVNKEDLTIEIPR
                     YSINHAKENH"
     gene            27530..28264
                     /gene="dnaQ"
                     /locus_tag="PKV30_00165"
     CDS             27530..28264
                     /gene="dnaQ"
                     /locus_tag="PKV30_00165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRL28666.1"
                     /translation="MSRQIVLDTETTGLSAEGGDRIIEIGCVELVARKLTGNNLHFYI
                     NPERDSHEDALKVHGISNEFLRDKPKFAEVAEDILRYVDGAEIIIHNAAFDVGFLDKE
                     LERLGKPVFTSFVGEVTDTLRMAKQLYPGKRNSLDALCDRLGVDNSGRTLHGALLDAE
                     LLADVYINLTRGQEALLIDLGDVGAQQGAGQRIDLTVFELPVLCANAQEVAAHEEVLL
                     QIDKGSGGKTVWRLFDVAVGAPAAGI"
     gene            28292..28366
                     /locus_tag="PKV30_00170"
     tRNA            28292..28366
                     /locus_tag="PKV30_00170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28324..28326,aa:Val,seq:cac)
     gene            28517..29116
                     /locus_tag="PKV30_00175"
     CDS             28517..29116
                     /locus_tag="PKV30_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRL28667.1"
                     /translation="MLLHADDCQLVLIDYQARLMPAIHDAPAVLANARRLAQMAKLFD
                     VPVWATEQNPTKLGATAESLAPLCDSIVAKMAFSGADELQPLLAPPVAAPRGNARSLP
                     KHLQKPQTAAPERGTVVIAGCETHVCLLQTALMLLEAEWDVCVVTDACGSRTERNRDA
                     AYDRLAGAGCELVTTEMVGFEWLRDCEHPQFRAVQALIK"
     gene            29243..29662
                     /locus_tag="PKV30_00180"
     CDS             29243..29662
                     /locus_tag="PKV30_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28668.1"
                     /translation="MAFSKQLRLSRDTAQMAAAVPQVIAHRVGRMMTAGILPNGRDQQ
                     EFYMMGAEKVAAFNESWSAMSLQAVAAQQQFALWWVQTWWKVAMGGWMNPPSLQHLSN
                     SAQQRLFDSMLDVTSRGMAPVKRRAVANARRLNRATR"
     gene            29796..31787
                     /locus_tag="PKV30_00185"
     CDS             29796..31787
                     /locus_tag="PKV30_00185"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HRL28669.1"
                     /translation="MKNSKTGRRTRWQPWRAAAPRHTTGAEETSMTSYTDFYRRSIDD
                     RDAFWAEQAQLIDWQTPPQTICDYSKPPFAKWFVGGTTNLCHNAVDRHLKDRADQPAL
                     IFVSTETDQEKVYTFRELHAEVQRMAAVLKGLGVQKGDRVLIYMPMIAEACFAMLACT
                     RIGALHCVVFGGFASGSLATRIDDAEPKVIVSADAGSRGGKPVPYKPLLDEAINLSKH
                     KPDAVLLVDRGLSTMNLVAGRDRLWGAEREKHLNTEVPCEWVDATHPSYTLYTSGTTG
                     KPKGVQRDTGGYAVALAASMKYIYDGRPGETFFSTSDIGWVVGHSYIIYGPLIGGMAT
                     IMYEGLPTRPDGGIWWQLAEKYKVTVMFSAPTAIRVLKKQDPEYLTKYDLSKLRALFL
                     AGEPLDEPTAQWISEGLKGKPIIDNYWQTETGWPILTICNGVEKAPSKFGSPGKAVYG
                     YDVKLLDDQTGEELTGPDQKGVLAIEGPLPPGCLQTVWRDDARFVNTYWSSVPNKLVY
                     STFDWAIRDQDGYYFILGRTDDVINVAGHRLGTREIEESIAAHPNIAEVAVVGVADQL
                     KGQVAMAFAVLKDASSLGDEAAMLKLEGEVMKLVDGSLGAVARPARVRFVSVLPKTRS
                     GKLLRRAVQAVCEGRDPGDLTTMEDPAALQQIKDLVAPA"
     gene            31995..>33681
                     /locus_tag="PKV30_00190"
     CDS             31995..>33681
                     /locus_tag="PKV30_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRL28670.1"
                     /translation="MSETKSVYQTYQGNTYLFGGNAPYVEEMYESYLANPGSVPDSWR
                     EYFDALQHVPATDGSNAKDVPHLPVINAFAERAKAGGTQVVQASGADSELGRKRTAVQ
                     QLIAAYRNVGARWADLDPLKRAERPNIPELEPSFYGFTDADMETVFNTSNTFFGKDTM
                     PLRELLNALRETYCGTIGAEYMYITDQGQKRWWQQKLESARANPQLSAEQKKHVLERL
                     TAAEGLERFLHTKYVGQKRFSLEGGESFIVAMDELVNQAGKQGVQEIVIGMAHRGRLN
                     VLVNTLGKMPGMLFAEFDHTAPEELPAGDVKYHQGFSSDVTTAGGPVHLSLAFNPSHL
                     EIVNPVVEGSVRARLDRRGDTEGDTVLPVLVHGDAAFAGQGVVMETLALAETRGYYTG
                     GTVHIVINNQIGFTTSDPRDSRSTLYCTDIVKMIDAPVLHVNGDDPEAVVLCTQLALQ
                     YRQDFNKDVVVDIVCFRKLGHNEQDTPMLTQPLMYTKIGKHPGTRKLYGEKLVTQGVL
                     PADGPDQMVATYRAAMDAGKHTVDPVLTNFKSKYAVDWSPFLNQKWTDAADTAI"
BASE COUNT         6237 a        11044 c        10981 g         5419 t
ORIGIN      
        1 ctcgcggccc atgcggcgca tctcgccctt gatgacgttc tgcaccaggc cgtcgtactg
       61 gccgccgcgc gggccggtgc ggccaaacag cgcttccttg ccccacttaa gggcgtcgcc
      121 gaccagcccg ccgtcttcgg ccgccggcgt tgctgcgtcg ccagacgctg cgccggtgcc
      181 ggagactgtc accgcacctg gcttcacggc ctgcgcgccg cgctcggcca gcttctcgta
      241 ggccgattcg cggtccaccg cctcttcgta agtgccggcg accagcgacg acttgatcag
      301 ctcggcgcgc tgcgcgtcgg tgatcgggcc aatctggctg ccgggcggga tgacgtacac
      361 gcgctgcgtg atgccggggc ggcctttttc gtcgagaaag ctgaccagcg cctcgccggt
      421 ggccagttcg gtgatcgcgg tttcgatgtt gagttgcggg ttgccgcgca tcgtctcggc
      481 cgtggccttc accgccttct ggtcgcgcgc cgtaaacgcc cgcaacgcgt gctgcacgcg
      541 gttgcccagc tgcgccagca cggtgtcggg cacgtcgatg gggttttgcg tcacgaagta
      601 cacgcccacg cctttcgagc ggatcagccg caccaccagc tcgatgcgct ccagcagcac
      661 cttgggcgcg tcgttgaaca gcaggtgcgc ttcgtcaaag aagaaggcga acttgggttt
      721 ctcggggtcg ccaatctcgg gcaggcgctc gtacaaatcg ctcagcatcc acagcaagaa
      781 ggtggcgtac aggcgcggcg attgcatcag cttgtcggcg ctcaggatgt tgacgacgcc
      841 ctggcccgac acggtctgca tcatgtcgtc gaggttgagc atcggctcgc cgaagaattt
      901 gtcgccgccc tggccctcca gcgtgagcag gccgcgctgg atggcgccca cgctggcggc
      961 gctgacgttg ccgtattgcg tggtgaactg cttggcgttt tcgcccacga aggtcagcat
     1021 gctgcgcagg tccttcaagt cgagcagcag caggccgttg tcgtcggcga tcttgaacac
     1081 caggttgagc acacccgtct gcgtctcgtt caaatcgagc atgcgcgcca gcaacagcgg
     1141 ccccatgtcg ctgatggtgg cgcgcaccgg gtggcccatc tcgccgaaca cgtcccacag
     1201 cgtcaccggg caggcgacgg gttcgggcgt cggcaggccg cgctccttca gcacatcggc
     1261 catcttgccc ttgatgttgc cgggctggct gatgccggtc aggtcgccct tcacgtcgct
     1321 catgaacacc ggcacgccga tctggctgaa cgattcggcc agcttttgca gcgtcaccgt
     1381 cttgccggtg ccggtggcgc cggtgatcag gccgtggcgg ttggccagcc cgggcaggat
     1441 gaagcactgg atgtcgccat gctgggcaat gaggatcggg tcggccatgt gaaggggcgc
     1501 ttgagcgcgc gaaaagtaga atcgcacgat tatcccgaac cttcaaaaag gcagagtttc
     1561 atggccggcc acagcaaatg ggcgaacatc cagcaccgca agggcaggca ggacgaaaag
     1621 cgccagcgca tctggacgcg cgtggtgcgc gagatcatgg tggccgcgcg caccggcggc
     1681 ggcgatccca ccgccaaccc gcgcctgcgg ctggcgattg aaaaagccaa ggccgccaac
     1741 atgccggccg acaccgtcaa gcgcaacatc gacaaggcca ccggcaacct cgaaggcgtg
     1801 agttacgaag agattcgcta cgaaggctat ggcatcggcg gcgccgccat catcatcgac
     1861 accatgaccg acaaccgcgt gcgcaccgtg gccgaagtgc gccacgcgtt cagcaagtac
     1921 ggcggcaaca tgggcactga gggctcggtg gcgtttcagt tcaagcacgt cggccagctc
     1981 atctttgcgc ccggcgtgag cgaagacaag gtgatggaag tggcgctgga agccggcgcc
     2041 gacgacgtca tcaccgacga cgaaggcgcc atcgaggtgc tgaccgatcc ggccgctttc
     2101 gaggcggtca agaacgcgct ggaagccgcg ggcctgacgc cggagctggc cgaagtgacc
     2161 atgcgcgccg agaacaccat cgacctgacc ggcgacgatg cggccaagat gcaaaagctg
     2221 ctcgacgtgc tggaagacct ggacgacgtg caggacgtgt ttcacaacgc caacgtggag
     2281 gcggtgtgag atttgccgct tggctgacca cgctgctgct ggtcggctgc ggtggcggca
     2341 gcgaggtatc gtggtgcatc ggcagcggat cgggcggcgt ctcggccggc tacaacgcca
     2401 cccagtgccc acctgaatcg ccggccaaga attccacctc tgcagtgtcg tattgaatca
     2461 aattggcctc cagcgcttgt aggataagcg caagcagctc ctgtttttga agtaacccca
     2521 tgaagatcct cgtcatcggc tccggcggac gcgagcacgc gctggcctgg aagctgtcgc
     2581 aatcgcccaa ggtgcagcag gtctacgtcg cgcccggcaa cggcggcacg gcgctgtcgc
     2641 ccaacctgaa gaatgtgccc ctcaccgact tggccgcgct gcgcgactgg gcgttggccg
     2701 agaagatcgc gctcaccgtc gtcgggcccg agcagccgct ggccgccggc gtggtggacg
     2761 actttcgcgc ccacggcctg cgcatcttcg ggcccaccca ggcggcggcg cagctggaaa
     2821 gctccaaggc cttcagcaag gacttcatgg cgcgccacgg cattcccacc gcagcgtacg
     2881 aaaccttcag cgatccggcc gccgcgcacg cctatgtcga ccggctcggc gcgcccatcg
     2941 tcgtcaaggc cgatggcctg gcggcgggca agggcgttgt cgtggctgct accaaagaag
     3001 aagcgcacgc ggccatcgac gacatgctgg gcggcaacac gctgggcgtg gcgcacaacg
     3061 acggcggcgc gcgcgtcgtc atcgaagaat tcctgcaggg cgaagaagcc agtttcatcg
     3121 tgctgtgcga cggcaagaac gtggttgcca tggccagcag ccaggaccac aagcgcctga
     3181 aggacggcga cgaaggcccc aacaccggcg gcatgggcgc ctactcgccc gcgccggtgg
     3241 tcacgcccga cgtgcatgcg cgcgccatgc gcgacgtcat cctgccgacc attcgcggca
     3301 tggacaagga cggcatcccg ttcaccggct ttctgtacgc cggcctgatg atcgatggcg
     3361 acggaaaacc gaagacgctg gagttcaaca cacgaatggg cgaccccgag acgcagccga
     3421 tcatgatgcg gctcaagtcc gatctggtcg acgtgctgat ggccgccacg tcgggcaagc
     3481 tggacgaggt ggaactgaaa tgggatcgcc gcgtagcgct gggtgtggtg ctggcggcgg
     3541 gcggctatcc gctgtcgccg aagaagggcg acctgatcag cggcttgcct aaacctacag
     3601 acgaggtggt ggtgttccac gccggcaccg tggccgaagg cgatcaggtc aagacggccg
     3661 gcggccgcgt gctgtgcgtc accgcgctgg ccgactcggt caagcaggcg cagaccaagg
     3721 cctacgacgc ggcgcgcacc atccacttcg atggcatgca gtaccggcgc gacatcggcc
     3781 accgcgccgt caaggccagc cactgacatg gcggccatcg aaccgcgcgc cgtgcgcgac
     3841 tacctgatcg gcctgcaagc gcgcattacc cacgccatcg agtcgcgcga cggccaggcg
     3901 acgtttttga cagatgcgtg ggccaaggcg cccggcgagc cgctgcaggg cgacggcatc
     3961 acgcagattc tggagggcgg cgccgtgttc gagcgcgccg gctgcggctt ctcgcacgtg
     4021 cgcggcccca agctgccgcc atcggccacg cagcaccggc ccgaactggc cggcgcgccg
     4081 ttcgaggcca tgggcgtgtc gctggtgttc cacccgctga acccctacgt gcccacggtg
     4141 cacatgaacg tgcgcatgat cgccgccggc cacccgggcg cggcgccggt gtgctggttt
     4201 ggcggcggta tggatttgac gccgtactac ggcttcgacg aggattgcac ggacttccac
     4261 cgcgtgtgcc gcgatgcatt gaagcctttt ggcgacgaca agtatccgcg cttcaagcaa
     4321 tggtgcgacg agtatttctt caacaagcac cgcagcgagc cgcgtggcat cggcggcatc
     4381 ttctttgacg atttttcaga gggcgacgcg gcccacggct ttgcgctgat gcgctcggtg
     4441 ggtgatgcgt ttctggatgc gtacgtgccc atcgtgcagc gccgcgtcgg catgccgtgg
     4501 ggcgagcgcg cgcgcgagca ccagttgatc cggcgcggtc gctacgtcga gttcaacctg
     4561 gtgtgggacc gcggcacgca cttcggcctg cagtcgggcg ggcgcaccga atcgatcttg
     4621 ctgtcgatgc cgccccaggt ggcgtgggcc taccagcggc aaccgttgcc gggcacgccc
     4681 gaggccgcgt tgaccgagcg ctacctgcaa gcgcgggagt gggtttgagc gccgcgccgc
     4741 tgcgcatcgg tttgttcggc ggcgccttcg atccgccgca cgacgcgcac gtggcgctgg
     4801 cgctggcggc catcaaccag ctggcgctgg atcggctgta cgtcgtgccg accggcgacg
     4861 cgtggcacaa gagccggccc ttgagccccg gcgccggtcg gctggcgatg tgtcgcctgg
     4921 cgtttgcgca cataccccag gtggccgtcg acgaccgcga actgcggcgc gacggcccca
     4981 cctacaccat cgacacgctg cacgagttgc gcgccgaaca cccgggcgcc gagctgttct
     5041 tgcagatcgg tgccgatcag gcggcggcgt ttcacacctg gcgccgggcc gatgaaatcc
     5101 tgcagattgc tacgctatcg atagctgttc gcgcttatga ttcaagcgct ggcgatgttt
     5161 ttgacccaaa gaacccgttg cctggcctgg ccacgggtgg cgcgcaggtg cgcatcctca
     5221 gcctgcccgc catgacgcac agcgccaccg atgtgcgcca ccgcgtggcc gaagggctgc
     5281 cgatcgacca tctggtgccg cccgcggtcg ccgggtatat tgccgaacac cacctttacc
     5341 aagagcctag atgagcgaaa ccgccgccaa gaaagacgtc cagaaactcc agcgcgccat
     5401 cgtcgatggc ctggaagacg tcaaagccca ggacattcgc gtgttcaaca ccgagcacct
     5461 gtcgccgctg ttcgagcgcg tgatggtcgc cagcggcacg tccaaccggc agaccaaggc
     5521 gctggccacc agcgtgcgcg acaaggtgcg cgacgccggt tttgccaagc cgcgcatcga
     5581 gggcgaggag aacggcgaat ggatcatcgt ggactgcggc gtggccgtgg tccacatcat
     5641 gcagccgacc atccgccagt actacaacct ggaagaaatc tggggcgaaa agccggtgcg
     5701 gctcaagggt ccggccagca aggcggcggt gaaccggcgc gcggcgaccg caccttcgaa
     5761 agaaaaagcg gccccagcgc ccgccaagaa agcgccagca gctaccaaaa aagcagcacc
     5821 agcgaagaag ccagcgacca aggtcgctgc caagtcgccg gcccgaaagg cgcccgccaa
     5881 gtcgcccgcg cgcaagagtg ccgcgccgcg cggcgcacgg ggcagcgcgt catccacgct
     5941 gcaaaccgtg gtggtgaaca agccctcggc gcgcgccgcc gccaaggcag cggccgcgca
     6001 gcccatgggc agggcgcccg ccaaagcccc ggcgaagaag gcgccggcgc gagccgcccc
     6061 caagggggtc gccaagcccg ccgcgcgcaa gaccgcgcca cgcaccagcc gctgacgcgc
     6121 ggctatgaag ctgctggtca tcgccgtcgg tcaacgcatg cccgattggg cgcagaccgc
     6181 gtgggccgac tacgccaagc gctttccgcc cgagttgcgg ctggagctga aggcgatcaa
     6241 gaccgagccg cgctcgggcg gcaagaccac cgagcagatc atgaccgccg agcgccagcg
     6301 catcgaggcg gccattccgt ccggcacgca cgtggtggcg ctggacgagc gcggcagcgc
     6361 cctcacgacg gccgcgctgg cggccaagct gagccagtgg caagggcagg gcggcgatgt
     6421 ggcgctgctc atcggcggcc cggacggcct gcatccggcg ctgcgccagg cggcgcacga
     6481 gcgcatccgc ctgtccgatt tgacgctgcc gcacgccatg gtgcgcgtgc tgctggtcga
     6541 gcagttgtac cgcgcgtggt cgatcaacgc cggccacccg taccaccgcg actgattgct
     6601 ggtgcgcttg cggcggcgcc taagatgcgt gcatggtcga cttcatttac ctcgcctcgc
     6661 aaagcccgcg ccgccgccag ttgctggagc agctgggcgt gcgcctgtcg ctgctgctgc
     6721 cggacgacag cgacgcggcc gaggcgctgg aggcggtgcg cacgggcgag gcgccggcca
     6781 cctatgtgcg ccgcgtgacg cgcctcaagc tcgatgacgc ggtgcgccgc ctggccgagc
     6841 aggcccggcc tgcggcgccg gtgctgtgcg ccgacaccac ggtggcgctg ggccgcgcca
     6901 tcctcggcaa gccgcgcgat gcgcaagacg ccgcggacat gttgcgccag ctgtctggcg
     6961 acacgcaccg cgtgctcacc gcggtggccg ttcagcaggg cgactggcgc ggcgaggcga
     7021 tgagcgaatc gcgcgtgcgc tttgccacct tgcgcgatga cgacatcaag gcttatgtcg
     7081 ccagcggcga atggcaaggc aaggccggcg cctacgccat tcagggccgc gcggcggcgt
     7141 tcatcgaaca catcagcggc agctacagcg gcatcatggg cctgcccctg tttgaaactg
     7201 cggctttatt gcgcgcagcg cacgccaatt aagcgcaagc agctatacaa aatgcagcaa
     7261 gaaattctca tcaactggtc gccgcaggaa acgcgcgtgg ccgtcatcga aggcggcgcg
     7321 gtgcaggagc tgcacgtcga gcgcacgctg gagcgtggcc tggtcggcaa catctacctt
     7381 ggcaaggtgg cgcgcgtgtt gccgggcatg cagtcggcct tcatcgacat tgggctggag
     7441 cgcgcggcct tcctgcacgt gggcgatctg tggcaaaagc cgctcgaagg cgagagcctg
     7501 cccgcgcatc gcagcggcgg cccgctcaag ccgattgaaa agcaggtgtt cgagggccag
     7561 acgctgatgg tgcaggtcat caaggacccg attggcacca agggcgcgcg cctggcgacg
     7621 cagatcagca ttgccgggcg catgctggtg tttctgccgc aggacgacca catcggcgta
     7681 tcgcaaaaaa tccccgccgc gcagcgcgag cagctgcgca cgcgcgtgca gacgctggcg
     7741 ggcgaggcgg cggacggcgg caaggcgggc ggcttcatcc tgcgcacgaa tgcggaagac
     7801 gccagcgatg ctgagctggc cgaagacatc gcctacctgc gcaaggcgtg gtcgcgcatc
     7861 cgcgaagccg ccgcgcgcca gccgccggcc agcctgctgc acgaagacct gagcttgctg
     7921 cagcgcgtgc tgcgcgattt gacgggcgag cacacgcaat cgatccgcat cgattccagc
     7981 gagcagtttc acgcgctggc cgcgttcggg cgcgagttca tgccggccgc cgccgcgcat
     8041 ttgcagcact acaagggcga gcggccgatc ttcgatttgc actcggtcga cgaggagatc
     8101 accaaggcgc tgggacgacg cgttgatctg aaatccggcg gctatctgat catcgaccag
     8161 actgaggcgc tgaccaccat cgacgtcaac accggcggct ttgttggcgc gcggaatttc
     8221 gacgacacgg tgttcaagac caatctggag gcggcgcaag ccattgcgcg ccagttgcgc
     8281 ctgcgcaacc tgggcggcat cgtcatcacc gacttcatcg acatgacgca gccggagcac
     8341 cagagcgcgg tgctggccga gtttcgcaag caactggcgc gcgaccgtgt gaagacgcag
     8401 gtgggcggct tctcgtcgct gggcctggtg gagatgacgc gcaagcgcac gcgcgaatcg
     8461 ctggcgcaac tgctgtgcga accgtgcgag caatgccagg gcgccggccg cgtgcgcacc
     8521 gcgcgcaccg tgggctacga catcctgcgc gaaatcctgc gcgaggcgcg gcagttcaac
     8581 cccaaggagt ttcgggtggt ggcagcgccg gtggtgatcg acctgctgct ggacgaagag
     8641 agcacgcacc tggcggggct gtcggacttc atcggcaagc cgatctcgct gcaggccgaa
     8701 agcgcgctgg ggccggacca gtacgacatc gtgttgttgt gatcttcaga aaaaatagcc
     8761 gctagcgctt gactggcttg cgcaggcagc tattcgtttg atagctcacc cggatggctt
     8821 tgcagccgcg catacaagcc gcccgccgcc atcaactgcg catgcgagcc ctgctcgacg
     8881 atgcgcccgc gctccatcac cacgatgcgg tcggcgtgct cgacggtgga cagccggtgg
     8941 gcgatgacca gcgtggtgcg cccggccatg gcgcgcgtga tggcctgctg caccaggcgt
     9001 tcgctttcgg tgtcgagcgc gctcgtggcc tcgtccagca gcagcaccgg cgcgtccttg
     9061 tacagcgcgc gggcaatggc caggcgctgg cgctggccgc ccgacaactg cgtggcgttg
     9121 tggcccagca cggtatcgat gccttgcggc aggctggcca cgtgggccgc cagattggcg
     9181 tcctgcaggc agcgctgcac gcgctcgcgg tcgatgctgg cggcgccgag cgccacgttg
     9241 gcggccacgg tgtcgttgag catggtcacg tcctggctga ccagcgcaaa ttgcgcgcgc
     9301 agggcggtca gtggccagtc ggcgatgtcg cggccatcca gcaatatctg gccgctgtcc
     9361 gggcgcacaa agcgcggcag caggttgacc agcgtggtct tgcccgatcc cgacgggccg
     9421 accagtgcca cggtttcacc ggcgccgacg tccaggctca gcgcgttcag cgatggctcg
     9481 gcgccattgg cgtagatcac ggtgacatcg cgcaacgcca gttcgccgcg cgcgcgggcc
     9541 ggcggcgtgg cggacgcggg cggcccgact tcgggctgca cctggtccat caggtccagc
     9601 ccacgctcca gcgcggccag gccgcgcgtc accgggccga ccacgtcggt cagccggcgc
     9661 agcggctgga tcagcatcag catggcgccg atgaaagaca caaagccgcc gaccgacatg
     9721 ccgcgcccgc cgctggcctg gccttgctgc agtgcaatgg cgatgaccac cgacagcgcg
     9781 accgccgccg ccatctgggc cagcggcatg gccgcggccg aagcgctggt cgccttgatc
     9841 gacacaccgc gcagcgcacg gctcagggtg tgaaagcgct ccgcctgctg ctgctgcgcg
     9901 ccgtgcagac gcaccatgcg gtgcgccagc acgttttctt cgaccacgta ggccagttcg
     9961 tcggtggtgc tttgcgacag gcgcgtgtag cggtacaggc gccgggacat ggtcttcatg
    10021 atcagcgcga cgatgggcac cagcacgagc acaatcagcg tcagcgacca gttcaaataa
    10081 agcaggtaac ccatcaaggc cagcaccgaa aaactgtcgc gccccaggtt gagcagcgat
    10141 tgcaccagca tggtggtgcc cgtctgcact tcgtagacga tggtgttggc gagctggctg
    10201 gcgctctggc gcgcaaacag tgccagatcg gcgttcaaca gccggtcgaa cagctgccgc
    10261 cgcagcttga ccatgccttc gttggcgatg cgtgccagcg cgtactggct ggcaaagtgc
    10321 gccacaccgc gcagcgcgaa cagcccgacg atggccagcg gcacggtcca gatcggcagg
    10381 ctgccttggg taaagccttc gtccagcagc ggcttgagca gtgccggcac cgaggcttcg
    10441 gtgagcgcgc tgatcagcgt gccggcaatg gccagcaccc agcccaggcg gtactcgctg
    10501 aaataggtcc acagacgggc cagccgtgcg ccccagggcc gctgggcggc gggcgaggcg
    10561 ggtggctgaa tgcgggcgtc gttcatggct aacggggcgg cgggtggccg gggcattcta
    10621 cggagcgctg cgtttcggcc cacggcgcgg gaacttccgc gcgccacatt ggacaaattc
    10681 acagtttgaa acgtcgctgc aaacaagctt gttaccattg cccgggccta tcgacccggc
    10741 aaacccatcg gtcttgccga agtacttcgt gatgaacacc gatcccccca ccagcaatcc
    10801 tcatcccaat tggcaacgcc gcggcctggt cgccgcgttg gcatccgctc ccttgtcggc
    10861 cgcttgggcc caattccgcg tcgaggtgtc gggcgtgggc ctgacccagc tgccgatcgc
    10921 gctggtgccg ttccgcggtg aagacgcggc gccgcagaag atcagcgcca tcatccgcgc
    10981 cgacctggag cgcagtggcc agttccgttc tgtcgatgcg agcggcgagg cgatggacga
    11041 gaccacgcgg ccagacatgt ctgtctggcg tcagcgcaca gccgacgcgc tggccggcgg
    11101 cagcgtcacg cgcctgccgg acggtcgcta cgacgtgcgc tttcgcctgt gggacgtggt
    11161 caaaggccag gatctgggcg gccaaggcta cgtcgtgccg caggccgact tgcgcttggc
    11221 ggcgcacaag attgccgact acatctacga aaagctcacc ggcgaaaagg gcgtgttctc
    11281 gacccgcctg gcctacgtga ccaaggccgg cagccgctac acgctttggg tggccgattc
    11341 agacggcgaa aacgcgcagg cggcgctggg cagccccgag cccatcattt cgcccacctg
    11401 gtcgccgacc ggcgcgcaac tggcctatgt gtcgtttgaa tcgcgcaagc cggtgatcta
    11461 cgtgcacgac gtgtccaccg ggcgccggcg actgctggcc aacttccgcg gttccaacag
    11521 cgcgccggcc tggtcgcccg acgggcgcaa cctggcggtc acgctgtcgc gcgacggcgg
    11581 ctcgcagatc tatctgatca gtgcctccgg cggcgagccg cggcgcctca cgcaatcgca
    11641 gagcatcgac accgagccgg ttttttcgcc cgatggcgcc agcatctact tcgtcagcga
    11701 ccgcggcggc gcgccgcaga tctaccgcat gcccgccagc ggcggcaacg cgcagcgcgt
    11761 gaccttcaac ggcagctaca acgtgtcgcc gactgtcagc ccggatggac gttatctggc
    11821 atacgtgtcg cgcaccggtg gctacaaatt gcaggtgatg gaactggcct ccggcaacgt
    11881 cacggctgtc accgacacag cggaagatga gaaccccagc tttgcgccca acagccgcct
    11941 gatcgtctac gcgacccggc acggcgggcg cgaggcgctg atgaccacca cggtcgacgg
    12001 caagatcaag gcgcgccttg cagggcgcag cggcgatatt cgtgagccgt cgtgggggcc
    12061 gttccagaaa tgattcgaag ggctgccttc agtcctgctt tcctcaatcc gttcagttca
    12121 ggagtcgaaa aatgatgaag cgatatttga tggtggctgc gttggcggcg gtgctggccg
    12181 gctgtagctc gggcgtcaag cttgatccgc cgatccaggg cagcggcacc accggtgctg
    12241 atgcgagcgc caccaccggc gccaccggcc agggcgccgg ccagagcacg gtgacgcagg
    12301 tgcaggccgg ctcggtcggc gccgatgctg caggtcctgc cggcgtgggg cgcgtgatct
    12361 acttcgattt cgacagcttc agcgtcaagc ccgaatacca gtcgctggtc gaccagcacg
    12421 cgcgtttcct gcaaggcaat cggggccgca gcgtggcggt tgagggccac accgacgagc
    12481 gcggcagccg cgaatacaac ctggcgctgg gccaaaagcg cgccgaagcc gtgcgccgtg
    12541 cacttacgct ggtgggcgcc accgatgcgc agatcgaggc cgtgagcttc ggcgaggaga
    12601 agccggccgc atctggcgcg agcgaggacg cctactcgca gaaccgccgt gtcgagatcc
    12661 gctaccgctg aacgcgccgt gatgcgaatg acgtatttgc ggatcgcggt gctctctctg
    12721 gcttgcgtgg ccgggcagag cgcgcaggcg cagctgtttg gcgacgacca ggcgcgacag
    12781 gcgatcctgg atctgcggca acgcttcgac caatcggtgg cggcgcagaa ccgcctggtc
    12841 gatgaaaaca cccagatgcg ccgcagcatg ctcgacctgc agcagcagat cgaatcgctg
    12901 cgcggcgaac tggcccgctc gcgcggccag gaagaacaac tgacgcgcga cttgagcgaa
    12961 ttgcgcaagc tgcagaccag ccagcaatcg ctggaagacc ggctgcgcaa gctggaaagc
    13021 gcggaatcgg cgcgcttggc caccgaggcc gctgcgtcga gcacgcagtc cccgccgttg
    13081 cccagcgatt ctgcgggcgc cagcgaaaag caagactacg acgccgcgct ggcggtgttt
    13141 cgcgccggcg acttcaaggg ctcgcagacc gggtttgcca acttcatcaa gcgctatccg
    13201 cgcagcaacc tggcgccttc cgccctgttc tggctcggca acgcccagta cgcgacgcgc
    13261 gactacaagg aagccatcac caacttccgc tcgctgctga cggcatcgcc caaccaccag
    13321 cgcgcgccgg aggccatgct gtccatcgcc aactgccaga tcgagctgaa agacacgcgt
    13381 gccgcccgcg ccacgctgga atcgctggtc aagacctatc cgcagtccga agccgcgcag
    13441 gccggccgtg aacggctgag ccggttgaag tgacatcggg caggggtggc tggcccctgc
    13501 tataatcctt ggttctcttg ccgcaccccg aatcgacgcc gggggcggct ttttatcagc
    13561 cacaaggaga gccgatgcgt cactacgaaa tcattttgct gatccacccg gatcagagcg
    13621 agcaggttcc ggccatgctc gagcgctaca agggcatgat caccgccggc ggcggcaaga
    13681 tccaccgcca ggaagactgg ggccgccgtc agctggccta ccagatcaac aagctgggca
    13741 aggcacatta cctgtgcctg aacatcgagg cggatcaggc tgtgatggcc gaactggagc
    13801 acgccttcaa gttcaacgat gccgtgctgc gccacctgac ggtgcagcgc aagaaggccg
    13861 ataccgcccc atcgtcaatg atgaagaccg tcgagcgtga agaagcgcgc aaggcgcagc
    13921 aggcggaaac caccaactga tctggcgtca tcgcgcggtc aaggagaggt gaaccagacc
    13981 cggctcacgg ccagcatcgt cgagatcggc gctgtgcgcc tgacccccgc cggcgtgccg
    14041 gcactcgatg cccggctcga acacgagtcc gaggtcgaag aggggggcag cagccggcag
    14101 gtcaaggcgc tgctcaaggc ggtggccctg ggggccgtgg ccgaaaggct cggcaggcag
    14161 ccgataggct ccgcgtggtg tttcaccggt tttctcgcca gcccgcgcaa cgccaagtcg
    14221 gtggtgctcc acatccagga ttttcagcaa gattagattc aaggaggcct catggccacg
    14281 ttcaagaaat tcaacaagga caagcgcccc aagcgcaata cgcaatcgct gctgttcaag
    14341 cgcaagcgct tctgccgctt cacggtcgcc ggcgtcgaag aaatcgacta caaggacgtc
    14401 gacacgctgc gcgatttcat cggcgaaaac ggcaagatcg tccccgcgcg cctgaccggc
    14461 acgcgtgcct tctaccagcg ccaactcaac accgccatca agcgcgcacg ctttctggcc
    14521 atgctgccgt acagcgacca gcaccgtgtc tgaagaagga gtcatcacca tgcaaatcat
    14581 tcttctggac aaggttgtca acctcggcaa cctgggtgaa atcgtcaaag tcaaggacgg
    14641 ttacgcccgc aacttcctga ttccttcggg tcgggcgcgt cgcgccaccg aggctgccaa
    14701 ggccgagttc gaagcccgcc gcgccgaact ggaaaagcaa gccgctgaaa agctggcggc
    14761 tgcccaggcg caaggcgaaa agctgaacgg ccagaccatc aagctgacgc aaaaagccgg
    14821 cgttgacggc cgcctgttcg gctcggtcac caaccacgac attgccgacg agctgaaaaa
    14881 gcatggcttc gacgtgcaca agtcgcaagt tcgcctgccc aatggcccca tcaaggtcgt
    14941 cggcgataac accgtacagg tgtcgttgca caccgatgtg gacgccgaga tcacggtggc
    15001 ggtgtacggc gaaactgcct gatcaacccg ttatcttctg acaagccgcc cgccgccctg
    15061 gtggggcggc tttgtttttt gtgcgtccgt tttccacagc ctcggtcgct caattcacct
    15121 tcgttgcacc gctttcccac agctttgtcc catgacaagc gaggccgggc ggcgtagcat
    15181 ggactgcttt tcgtctcgcc cgcgcatgtc ctccgttttc ccttccagtt acgccgatct
    15241 ggccgacgac gcgccgaccg atcggcaagt cgcccaactg cgcattcccc cgcattcgct
    15301 cgaggccgaa tccagcgtgc tgggcggctt gttgctggac aacagcgcct gggaccgcgt
    15361 gggcgatgta ctcgtcgaca gcgatttcta ccgttacgag caccggctgg tgtattcagc
    15421 cgtgggcaag ctggtcaatg agaccaagcc ggccgacgtg atcacggtct acgaagagct
    15481 gcaacggcag ggtaaaggcg acgagatcgg tgggctggtc tacctgaacg cactggcgca
    15541 atacgtgccc agtgccagca acatccgccg gtatgccgag atcgtgcgcg agcgatctat
    15601 tctgcgcaaa ctggtttctg ccagcgacga gatcgccacc agcgccttca accccaaggg
    15661 cagggcggtg gacaagatcc tggacgaggc cgaaggcaag atcttcaaga tcggcgaaga
    15721 gggctcgcgc atgaagcagg gcttccaggc catggatggc ctggtggtgc aactgctcga
    15781 ccgcgtgcaa gagatggccg acaacccgaa cgacgtgacc ggcgttccca cgggtttcgt
    15841 cgacctggat cgcatgaccg cggggttgca ggccggcgat ctggtggtgc tcgcagcgag
    15901 gccttcaatg ggcaagacgg cgtttgccat caacattgcc gagcacgtgg cgctgaacga
    15961 gcagttgccg gtggcggtgt tttcgatgga aatgggtgct gcacaactgg cggtgcgaat
    16021 cgtcggctcg atcggccgca ttgaccaggg ccacctgcgc accggcaagc tgaccgatga
    16081 ggaatggcca cgcctgaccg aagcgatcga gcgcttgcgc aacgtcagcc ttcacatcga
    16141 cgagaccccc gggctcacgc cttccgagct gcgcgccaat gcgcgccgcc tggcgcgcca
    16201 atgcggcaag ctggggctga tcgtggtcga ctacctgcag ctgatgagtg gcagcagcag
    16261 cgacggcgaa aaccgcgcca cagagctggg tgaaatctcg cgcggcctga aggcgctggc
    16321 caaggagttg caatgcccgg tgatcgcgct ctcgcagctc aaccgcagtg tcgaaacacg
    16381 caccgacaag cgccccatga tgagtgacct gcgcgaatcg ggcgccatcg agcaggacgc
    16441 ggacatcatc atgttcattt accgcgacga ctactacaac aaggattcgc gcgaaccagg
    16501 cgtggctgag atcatcatcg gcaagcagcg caatggcccc accggcaccg tcaagctggc
    16561 gttcgtcaag ccgctgacgc gctttgagag cctggcgctt ggtgccagtg acgattttta
    16621 agcgaaatag gccgcgagcg cttgtccatc aagcgcaagc agctataaaa atagtagttt
    16681 tcacaaacaa gaaagccgcg acgcgtcgct actttttgct gggcgctggc actcagcgca
    16741 aggcttgcat caccttggcg atcgattcgc agacgtagcc gatgttcttc tcgttcagcg
    16801 ctgcgacgca catgcggccc gtgtcggtgc cgtaaacgcc gaactcgtcg cgcagcctga
    16861 ccatctggtc ttttgacagc cccgaatagc tgaacatgcc gacctgcgtg gtgatgaaac
    16921 tcatgtcctg cttgacgccg gcagccttca agccatcgac cagcgcctgg cgcatcgcct
    16981 tgatgcgctt gcgcatgccg gccagttctt cttcccattg cgcacgcagc ttggcgtcgc
    17041 ccagcaccat ggccaccacg gcgccgccgt gcgtgggcgg gttgctgtag ttggtgcgga
    17101 tcatgatctt gagctggctc agcaccttgt cggcttcttc cttcgaagcg cacaccacgg
    17161 acagcgcacc gacgcgctcg ccatacaggc tgaacgattt gctgaacgag gtagagacaa
    17221 acacgtccag ccccgccgcg acgaacttgc cgaccaccgc gccgtcttcg gtaatcccgt
    17281 cggcaaatcc ctggtaggcc atgtcgagga aggcggtcag cttgccggcc ttgacgacct
    17341 cgatcacctg tgcccactgg tccggcgtga ggtcgtagcc cgtggggttg tggcagcacg
    17401 cgtgcagcac gacgacggtg ccgggggctg cagctttcag gtcggccaac atgccgtcga
    17461 agttcaggcg gcgcgtggcg gcatcgtaat agcggtaggt gccaacgtcg aaaccggctt
    17521 gcgtgaacag cgcgcggtgg ttctcccagc tcgggtcgct gatcagcact ttggcgttcg
    17581 gattgagttt tttcagaaag tccgcgccga tcttcaagcc gccggtgccg ccgagcgcct
    17641 gcaccgtggc cacgcgacct tgcttgacaa cgtctgaatc ggcaccgaat accaacgcct
    17701 tgacgccatt gtcgtaagcg gcaatgccgt cgatgggcag atagccgcgt ggcttgggtg
    17761 tatccatcag cgccttttca gccgcctgca cgcaggctag aagcggcaac ttgccgtttt
    17821 cgtcgaaata cacaccgaca ccgaggttga ccttggccgg gttctgatcg gcggcaaact
    17881 gttcgttcag acccagaatg gggtcgcgag gggccatttc gacggcggag aacaaagaca
    17941 tggggcgatc ctggtgcaac ggaaaggcgg agaacgcgcg cgcgacgagc catgttgctc
    18001 gcatgcgcta cgctggcggg gaaaaccctt gaattttagc ggttcgcccc acaccgagct
    18061 tgtatgacca ccattgccgc ggccgacgcg ccgacatcgg gccagtttca gagctttccc
    18121 gattcccctt tccagcttta ccagccgtac ccgcccgcgg gcgaccagcc aacggcgatt
    18181 gaccagcttg tcgaaggggt cgaaaacggc gagagctttc agacgctgct gggtgttacc
    18241 ggctccggca agaccttcac catggccaac gtgatcgcgc ggctgggccg gccggccatc
    18301 gtgttcgcgc ccaacaagac gctggccgcg cagctgtaca gcgagttccg cgagttcttt
    18361 ccgaagaacg cggtcgagta cttcgtcagc tactacgact attaccagcc cgaggcctat
    18421 gtcccgcacc gcgatctgtt catcgagaag gactcggcca tcaacgaaca catcgagcag
    18481 atgcgcctgt cggccaccaa gagcgtgctg gagcggcgcg acgtggtcat cgtggccacc
    18541 gtgtcggcca tctacggcat cggcgcgccg gacgattacc tgcagatgcg gatgatcctg
    18601 aaggtgggcg gcaagcaggg ccagcgcgac gccatcgcgc aactggtgcg catgcaatac
    18661 cagcgcaacg acacagactt cagccgcggc accttccgcg tgcgcggcga caccatcgac
    18721 gtgtttcccg ccgaacacag cgaactggcg gtgcgcatgg agttgttcga tgacgaggtg
    18781 gagagcatca gcctgtttga cccgcttacc ggccgcatcc agcagaaggt gccgcgcttc
    18841 gtcatctacc cgagcagtca ctatgtcacg ccgcgcgaga aaacgctggc ggcggtggag
    18901 acgatcaagc aggagctgaa tgatcggctg aaattcttcg tcggcgaagg caagctggtc
    18961 gaggcgcagc ggctcgagca gcgcacgcgc ttcgatctgg agatgttggc cgagctgggc
    19021 cactgcaagg gcatcgagaa ctacacgcgc cacctgtcgg gcgccgcgcc gggcgatccg
    19081 ccggccacgc tgaccgacta cctgcccaag gacgcgctga tgtttctgga cgagagccac
    19141 cagatgatcg gccagctcaa cgccatgtac aacggcgaca aggcgcgcaa gaccacgctg
    19201 gtcgaatacg gctttcgcct gccttcagcg ctggacaacc ggccgctcaa gttcgacgaa
    19261 ttcgagcgcc gcatgcgcca ggtggtcttc gtcagcgcga cgccggccga ctacgagaag
    19321 acacacgccg gcaacgtggt ggagcaagtg gtgcgcccca ccggcctgat cgatcccgaa
    19381 gtcgaagtgc gccccgccac gcaccaggtg gacgacgtgc tgcaggaaat ccgcttgcgt
    19441 agcgacaagc gcgagcgcgt gctcatcacc acactcacta agcgcatggc cgagcaattg
    19501 accgagtacc tgacggacaa cggcgtcaag gtgcgctacc tacacagcga cattgaaacc
    19561 gtggagcgcg tggagatcat tcgcgacctg cggctgggcg cgttcgacgt gctggtcggc
    19621 atcaacctgt tgcgcgaggg gctcgatatc cctgaggtct cgctggtggc gattctggac
    19681 gccgacaagg aaggctttct gcgcgccgag cgcagcctga tccagaccat tggtcgcgcc
    19741 gcgcgcaacg tcaacggcaa ggcgattctg tacgccgacc gcgtgaccga gtcgatgaag
    19801 aaagccatcg gcgaaaccga gcgtcgccgc gccaagcaga tcgagttcaa caccgtgcac
    19861 ggcatcacgc cgcgcggcat cgtcaagcag gtgcgcgatc tgatcgacgg cgtctacagc
    19921 gacaagggcg gcggcaccga cagccaggtg ctggcggccg aaatggcgcg tgcgcgcgtg
    19981 gaggacatgt ctgagaaaga cgtgtcacgc gagatcaagc ggcttgaaaa actcatgctt
    20041 gagcacgcgc gcaacctcga attcgaacaa gccgcccgcg tgcgcgatca gttgcacgtg
    20101 ctcaagcaaa gcgcgtttgg cgcgcccggc gccgacaacc tgcgcagcat cgccgggcgc
    20161 agcgccgctt aggtccaggc gctgcgcttc gcaagacggc gaatcaaccg accttgaggt
    20221 tgttcgtcac gttcttcacg ccttcgacct cgcgcgcaag ttgcgacgcg cggtccttgg
    20281 cggcctgggt cggcgctggg ccgctcaggg tcaccgtacc gtccttggtg tcgacgtcga
    20341 tgcgcagcgc gctcaggtcg gcgtccttgg ccagcttggc cgagatcgat gcggtgatcg
    20401 ccgcgtcgtc caccgcagcc ttcaccgcgg cgccggcctg cgatgcctcg gcgcggatct
    20461 cgctggtggc gctggccgcg tcggccttca gctcctggcc ggcctgcttg gcatcggccg
    20521 ttgcgtctcg cgcggcggct gcagcgtcgc gaccggcttc ctgaacgtct gccttcattt
    20581 cgcggcccgc ttctgccgtc ttggcggcgg cctggtcgac ctggcgcgcg gcctgatcct
    20641 gctcgctctt gctgcaggcg ccgagtgctg cggccatcaa gacagccagc gccagtgcgg
    20701 cagggcgggt ggtgcgtgat ttcgtcatgg agggtctcct tgggttgaaa aaggtgggtg
    20761 gaaagaaatc agtgcctgac gtccaagcgg ttgcgcaccg ccaccacgcc atcggtggca
    20821 cttgccgcct gctcggcctg ttccttggcc gcgtccgacg cgacgctgcc gcgcagccac
    20881 accacaccgc cggcggtgta gagctggatc ggctggccgc gcagcgcggg ctggcgctgc
    20941 aggcgggcgc gcaccgctgc gctgatgagt tcgtccttgt cggccgcagg cgccggtgcg
    21001 cgcgcgccac tggtcgccgc cgaagcggct tcgcgcgctt ccttcgcctc cgcctcggct
    21061 tcggcggccg tgcgttctgc gtcgcgcgcc gctgcctggc tgcgcgatac ggcggtgtca
    21121 aaactgcccg ccgtcatcac gttcgaagcc gattgatcgt cccgcgcgca cgccgccaga
    21181 ccaccggcca gcagcagtgc gggcagggtg tggagcaggc gcgtgtacag ggtgttcatg
    21241 gataactgca gtcatggcca gcgccgtcgc ggccaagaaa atctgtcgat gccctgaatt
    21301 taggagcggc ccgcggccgc gcgtgtccgc gcgaaagccc cgtgtgtgta ggacaaacag
    21361 gtgcccattg cgagggccgc ggacgacgcc gcgccgcgat ctatagcccc acgccgcagt
    21421 gggaaatggt gtatacttga taaaaacact cggaattgaa tgcgcagggc tgttgctttt
    21481 aagtcacatg ctttcgagtc cactgaaacc acgccaagga acaccgacat gcgcctcacc
    21541 accaagggca gattcgcagt caccgccatg atcgacttgg ctttgcgcca agacaacggg
    21601 cccgtcacgc tggcggccat cagccagcgg cagcacattt cgctgtctta tcttgagcag
    21661 ttgttcggca agctgcgccg gcacgagctg gtcgaatcca cgcgcggccc cggcggcggt
    21721 tattcgctgg gccgcaaggc gagcgagatc accgtggccg acatcatcac ttccgttgat
    21781 gagccgctgg acgccaccca ttgcggcggc aaggaaaact gctacggcga cggcggccgc
    21841 tgcatgacgc acgatttgtg gacttcgctc aacgcccgca tgctcgagtt cctggaatcc
    21901 gtcacgctgc aaaagctggt ggacgaccag cacgccaacg gcatcgagat tgaagacaaa
    21961 cccgccacca agcgcgccat ttcgtcggcg ccggtggtca agccgatccg catcaacgcg
    22021 cccaactcgg tgtttgcgtt gggcggcatt actaccaaat aaatagctgc tcgcgcttga
    22081 ttggcaagcg ccagaggcct aaaacgttta aaaagcgaat cacccgcgat cgcagatgtc
    22141 cgccatgagc cagaccccgc atttccccat ctacatggac tacggtgcca ccacgccggt
    22201 tgacccgcgc gtggttgacg ccatggtgcc gtggctgtat gaacacttcg gcaacgccgc
    22261 cagccgcagc cacgcctggg gctgggaggc tgaaaaagcc atcgaaaacg cccgcgaaca
    22321 agtggccgac ttgattggcg ccgatccgcg cgaaatcgtg tggacttccg gcgccaccga
    22381 atcgaccaac ctggcgctca agggcgcggc caatttctac aagggcaagg gtaagcacct
    22441 gatcaccctg aagaccgagc acaaggccac gctggacacc atgcgcgagc tggagcgcca
    22501 gggctttgag gtgacctaca tggaagtgca ggaagacggc ctgctcgatc tggaaaagtt
    22561 caaggccgcg atccgccccg acaccattct ggccagcgtg ctgttcgtca acaacgagat
    22621 cggcgtgatt caggacattg ccgccatcgg cgccatctgc cgcgagaagg gcgtgctgct
    22681 gcatgtggat ggcgcgcagg ccacgggccg cgtgaacatt gatttgaaga cgctgccggt
    22741 cgatttgatg agcctgacgg cgcacaagac ctatggcccc aagggcgtcg gcgcgctgta
    22801 cgtgcgccgc aagccgcgcg tgcggctcga ggcgcagatg cacggcggcg gccacgagcg
    22861 cggcatgcgc agtggcacgc tgcccacgca ccagatcgtc ggtatgggcg aggcctttgc
    22921 catcgccaag aaacagatgc atgacgacaa cgcccacgcc gcgcgcctgc aaaagcggct
    22981 gctcgatggg ctgaaggacg tcgaccaggt gttcatcaac ggcagcctgg acaagcgcgt
    23041 gccgcaaaac ctgaacatga gcttcaactt cgtcgaaggc gagtcgctca tcatgggcat
    23101 caaggggctg gcggtgtcgt ccggctcggc ctgcacgtcg gccagtctgg agccgagcta
    23161 cgtgctgcgt gcgctgggcc gcagcgacga gctggcgcac tccagcctgc gcatgaccat
    23221 cggccgcttc acgacggaag aagagatcga ctacgcgatc aaaaccatcc gcgagaacgt
    23281 cgccaagctg cgcgaactct caccgctgtg ggagatgcac caggaaggga tcgacctgtc
    23341 caccatccag tgggccgcac attgagtttg gaatttgaat cgaattgacc gccggcgcgc
    23401 gccaatcaag cgcaagcagc tatcaataaa ggagtaatca acatggccta ttcagaaaaa
    23461 gtcattgacc actacgagaa tccccgcaac gtcggcagct ttgacaaggg cgacgacagc
    23521 gtgggcaccg gcatggtcgg cgcgccggcc tgcggcgacg tgatgaagct gcagatcaag
    23581 gtcaatccgc aaaccggcgt gatcgaagac gcgcgcttca agacttacgg ctgcggctcg
    23641 gccattgcct cgtcgtcgct cgtcaccgag tgggtgaagg gcaagacggt ggatgaagcc
    23701 gccacgctga agaacagcca gatcgccgaa gaactggcgc tgccgccggt gaagatccac
    23761 tgctcgatcc tggccgaaga cgccatcaag gcagcggtgg acgactaccg caagaagcac
    23821 gcggcctgat ctgccatggc gctgcagcac gcgagttccg gcgatgtgat cgatgtgcgg
    23881 ccgtacggcg cgcgcatcga tggcgcgcaa tccatcgcgc tgttcaagtc gacccagctc
    23941 gaagtgatgc gcctggtgct gcccgccggc caccgcatgc cgccgcacca cgtgccgggc
    24001 gaaatcacca tccagtgcct ggaaggcgtg gtgcgggtcg atgtggcggc tgcgtcacac
    24061 gacttgccgg cggggcattt gatgtatgtc gcgggcggtg tcgaacacgc gctcacggcg
    24121 atcgagtcgg cctcggtgct ggtcacgatc gcgctttgaa gttcacggaa atcggattcc
    24181 caggccatgg ccgtctctct cactgaagcc gccgcgcggc acgtcaccaa atacctcgcc
    24241 aagcgcggca agggcgtggg cgtgcgcctg ggcgtcaaga ccacgggctg ctcgggcctg
    24301 gcctacaagc tcgaatacgt ggacgaggta gcgcccgaag atgagctgtt cgagcagcac
    24361 ggcgtcaagc tgatgatcga ccccaagagc ctggcctaca tcgacggcac cgagctggac
    24421 tacgtgcgcg aaggcttgaa cgaaggtttt cgctttaaca acccgaacga gcgcgaccgc
    24481 tgcggctgcg gcgagtcttt ccgcgtctga tggcgccggg gcagctgggc gatctggccc
    24541 acgcccgcgt gggcgtgacg atcgacaacg agcgcgaggt gagggcgctc aacgcctggt
    24601 tccagcgctg gggcccgcgc atccggcgca tggacgatca aggctgcggt tgctgcctcg
    24661 acgcctggga cgtgcaggcc ccgcccgaag cgctggccga actgccgcgc ggaatggtac
    24721 gcccggcgct gcacgcggcc gcttgagttg ttgcccatga acctgcaatc caccgacttt
    24781 gagctgttcg acgtgccggc gcgctttgcg caagaccgcg cgcagctcga cgcgcgctgg
    24841 aagcaactgc agcgcgaagt gcaccccgac cgctttgctg cccagggcgc cgccgcgcag
    24901 cgggtggcca tgcaatggtc ggcgcgcatc aacgaggcgt atcagcgcct gaaatacccg
    24961 cagcgccgcg ccgcctattt gtgcgagctg cacggcgcgc cgatccaggc cgagaacaac
    25021 actgccatgc cggccgaatt cctcatgcag cagatggaat tgcgcgagca gatggacgac
    25081 gcagccagta caaaggattt ggacgaaatc agccgctcgt gcttgtctgc ccagcgcgag
    25141 ctgctgcaaa aaatagagca tcagatggac gtcgagcacg actggccggc cgccgcacaa
    25201 tccgtacgcg cactgatgtt tctggacaag ctggccgccg acatcgagcg gcggcatgag
    25261 acactcgaat cgaactgaaa tcccttgagc ccgcgcagcc ctcggcgcgc aggccgcacc
    25321 gaaacttcat ggcgctgctt caaatctccg aacccggtca aacgcccgac ccgcaccagc
    25381 ggcgcatcgc ggtgggcatc gacttaggga caacgcattc acttgtcgcc gccgtgcgcc
    25441 acggcgtggc agagtgtctg cccgacgagc agggccgcgt gctgctgcct tccgtggtgc
    25501 gctatttggc gggcggcggg cggcagattg gccacgacgc gttgattgat gccgtggcgg
    25561 atgccgaaaa caccatcgcc tcggccaagc gcctgatggg gcggcgcctc ggcgacatcg
    25621 tggggcgcga tcaactgccg tatcgcctgc tcgaccaacc cggcatggtc gccatccaga
    25681 ccgcgcacgg cgaaaagtcg cccgtcgaag tcagcgccga aatcctcgcc acgctgcgct
    25741 accgcgccga agacaccttc aacgacgacc tgtacggcgc cgtcatcacc gtgcccgcgt
    25801 actttgacga cgcgcagcgc caggccacca aggacgcggc caagctggcc ggcatccacg
    25861 tgctgcgcct catcaacgag cccacggcgg cggccatcgc ctacgggctc gacaacgcca
    25921 gcgaaggtgt gtatgccgtg ttcgatctgg gcggcggcac gttcgacgtg tcgatcctgc
    25981 gcctgacgca aggcgtgttc gaggtcatcg ccaccggcgg cgattcggct ctgggcggcg
    26041 acgattacga cgcggcgctg gccgactggg tgctggcacg caccggcgcc accgccgcca
    26101 cgccggccga caaggccgcg ctgaaggcgg cggcgcgccg ggccaaggaa gcgttgagtg
    26161 cttccgataa cgtagcgttc agcgcacgtc tgtcaagcac tgaagcccga tttgatctga
    26221 agcctgaaga tctcgaagcc gccaccgccg cgctcaccgc gcgctgcatg acgaccgtgc
    26281 gccgcgtgct gcgtgacgcc aagctggcgc cggatgaaat caaaggcgtg gtcatggtcg
    26341 gcggctccac gcgcatgccg gtgattcgcc gcgcggtggc cgaattgttt ggccgcgagc
    26401 cgctcaccaa cctgaacccc gacgaagtgg tggcactcgg cgccgccata caggccaacc
    26461 aactggcggg caatgattcg gcgggcgatt tgttgctgct tgacgtgatc ccgctctcgc
    26521 tcgggctgga gaccatgggt ggcctggtcg agcgcatcgt gccgcgcaac cagaccattc
    26581 cggtcgccat ggcgcaggat ttcacgactt acaaagacgg ccagaccgcg ctggcgctgc
    26641 acgtggtgca gggcgagcgc gacctcgtgc aggactgccg cagcctggcg cggtttgagc
    26701 tgcgcggcat tccgcccatg gcggccggcg ccgcgcgcat ccgcgtgacc ttcaccgtcg
    26761 atgccgatgg cttgctgaac gtgtcggcac gcgagcagat cagcggggtc gaggcgcaga
    26821 tcgacgtcaa gccgagctac ggcctgagtg acgagcagat cgcgcaaatg ctgcaggact
    26881 cgttcagcac cgccgaggcg gacgtgaaag cacgcgcgct ggtggaggcc aaggtcgatg
    26941 ccgaccgcat gctgctggcc acgcaaagcg cgctggctgc cgatgccgcg ttgctcgctg
    27001 aatcagagcg cgccgagatt gacgcgctga tgctgtcact gcgcggcatc gccgccaaca
    27061 gcgtagatgc cgccgccatc gaggcggcca cgaaagcgct ggccgacggc accgaagcct
    27121 ttgccgccgc gcgcatgaat gccggcatcc agcgggcgct ggccggcaag tctttggagt
    27181 cgatttaagc catgcccacc atcaaagttt tgcctcatcc cgaatacgcg cccgagggcg
    27241 ccgagattca ggccaatctc ggcgattcga tctgcgaggc gctgctcgac aatggcatcg
    27301 agatcgagca cgcctgtgag atgagccgcg cctgcaccac ctgccactgc atcgtgcgca
    27361 agggatttga ctcgctgcaa gaagccgagg aagaggagga ggatttgctc gaccgcgcct
    27421 ggggcctgga gccgcaatcg cgcctgtcgt gccaggccat cgtgaacaag gaagatttga
    27481 cgatcgagat tccgcgctac tcgatcaacc acgcgaagga aaaccactga tgtcgcgcca
    27541 gatcgtcctc gacaccgaga ccaccggcct gtcggccgaa ggcggtgacc gcatcatcga
    27601 gatcggctgc gttgaactgg tggcacgcaa gctgaccggc aacaacctgc acttctacat
    27661 caaccccgag cgcgatagcc acgaagacgc gctcaaggtg cacggcatca gcaacgagtt
    27721 tctgcgcgac aagcccaagt tcgccgaggt ggccgaggac atcctgcgat acgtcgatgg
    27781 cgccgaaatc atcatccaca acgccgcctt cgacgtcggc tttctcgaca aggaactgga
    27841 gcggcttggc aagcccgtgt tcaccagctt cgtcggcgag gtgaccgaca cgctgcgcat
    27901 ggccaagcag ttgtaccccg gcaagcgcaa cagcctggac gcgctgtgcg atcgcctggg
    27961 tgtcgacaac tccggccgca cgctgcacgg cgcgctgctg gatgcggagc tgctggccga
    28021 tgtctacatc aacctcacgc gcgggcaaga ggcgctgctg atcgatctgg gcgatgtggg
    28081 cgcgcaacaa ggcgctggcc agcgcatcga tctcacggtg ttcgagttgc cagtgctgtg
    28141 cgccaacgcg caggaggtgg ccgcgcacga ggaagtgctg ctgcagatcg acaaaggcag
    28201 cggcggcaag acggtgtggc gcctgtttga cgtggcagtg ggcgcgccag cggctggtat
    28261 ataattccac gcttcgccga tgtggcgaga cgggtgatta gctcagcggt agagcactgc
    28321 cttcacacgg caggggtcgc aggttcaaac cctgcatcac ccaccagttt tcaaggctcc
    28381 ggtgctacaa acatcggagc tttttgtttt ttgcgcggcc gtcggtgcac tatcgcacgg
    28441 tgtcctgctt tagaggctgc aacccattgg cctttgcggc cgcaaagcgt ctaatccctg
    28501 ttttccccga aaccccatgc tgctccacgc cgacgattgc caactggtgc tgatcgacta
    28561 tcaggcccgc ctgatgccgg ccattcacga tgcgccggcc gtgctggcca acgcgcgccg
    28621 tctggcgcag atggccaaat tgttcgatgt gccggtctgg gcgaccgagc aaaacccgac
    28681 caaattgggc gccacggccg aatcgctggc gccgctgtgc gacagcatcg tcgccaagat
    28741 ggccttcagc ggtgccgacg aattgcaacc cttgctggca ccgccagtgg ccgcgccgcg
    28801 cggcaacgcg cgcagcctgc ccaagcattt gcaaaaaccc cagacggcgg cgcccgagcg
    28861 cggcacggtg gtgattgccg gctgcgaaac gcatgtctgc ctgctgcaga ccgcgttgat
    28921 gctgctggag gccgagtggg atgtgtgcgt ggtgaccgac gcttgcggct cgcgcaccga
    28981 acgcaaccgc gacgccgctt atgaccgcct ggccggcgcg ggttgcgagc tggtgacgac
    29041 ggagatggtc ggctttgagt ggttgcgcga ttgcgagcac cctcagttca gggcggtgca
    29101 ggcgctgatc aagtagcgcc tggtcaagtt ttcaagtgtt ttggggctcc agcgcttgtc
    29161 tggcaagcgc aagcagcttc tttatttgta gcgttttcag atcgtgcggt gccagtcgcc
    29221 ggccgaccaa ggagtttttc cgatggcttt ttccaagcaa ctgcgcctga gccgcgacac
    29281 cgcacagatg gccgccgccg tgccgcaggt gattgcccac cgcgtgggcc gcatgatgac
    29341 cgccggcatc ctgcccaacg gccgcgacca gcaggagttc tacatgatgg gcgccgagaa
    29401 ggtggcggcg ttcaacgaat cgtggtccgc tatgtctttg caggccgtgg cggcgcagca
    29461 gcagtttgcc ctgtggtggg tgcagacctg gtggaaggtg gcgatgggcg gctggatgaa
    29521 tccgccatcg ctgcagcatt tgtcgaacag cgcgcagcag cggctgtttg attcgatgct
    29581 ggacgtcacc agccgcggca tggcgccggt caagcgccgg gcggtggcca atgcgcggcg
    29641 actgaaccgc gcgacgcgct gattgctgcc agtccggcac gcgccatatg cgcgcgccgc
    29701 cgccggaaat ctctcgttcg aaccgatggc gggcgcccgc gcagcgctcg cagagttgac
    29761 gcacgtcagt atggcgcaag cgccatctcg ccataattaa gaattcaaaa acggggcgac
    29821 ggacgcgctg gcaaccatgg cgcgccgctg caccgcgcca tacaaccggg gccgaggaga
    29881 caagcatgac gagctacacc gacttttacc gtcgttccat cgacgaccgc gacgccttct
    29941 gggccgaaca agcccagttg atcgactggc agacgccgcc gcaaacgatt tgcgactaca
    30001 gcaagccgcc gtttgccaag tggtttgtcg ggggcaccac caatctctgc cacaacgcgg
    30061 tcgatcgcca cctgaaagac cgcgccgacc agccggcgct gatcttcgtc tcgaccgaga
    30121 ccgatcagga aaaggtctac accttccgcg agctgcacgc cgaagtgcag cgcatggcgg
    30181 cggtgttgaa agggctgggc gtgcaaaaag gcgatcgcgt gttgatctac atgcccatga
    30241 tcgccgaggc ctgctttgcc atgctggcct gcacgcgcat cggcgcgctg cattgcgtgg
    30301 tgttcggcgg ctttgcgtcg ggctcgctgg ccacacgcat cgacgacgcc gaacccaagg
    30361 tcatcgtcag cgccgacgcc ggttcgcgcg gcggcaagcc ggtgccctac aagccgctgc
    30421 tggacgaagc catcaacctg tcgaagcaca agcccgatgc ggtgctgctg gtcgatcgtg
    30481 gcctgtcgac tatgaatttg gtagctggtc gcgaccgttt gtggggcgcc gagcgcgaaa
    30541 aacacctaaa taccgaggtg ccctgcgaat gggtggacgc cacgcacccc agctacacgc
    30601 tgtacaccag cggcaccacc ggcaagccca agggcgtgca gcgcgacacc ggcggctacg
    30661 ccgtggcact ggcggcgtcg atgaagtaca tctacgacgg ccggccgggc gaaacctttt
    30721 tctcgaccag cgacatcggc tgggtggtgg gccacagcta catcatctac ggcccgctca
    30781 ttggcggcat ggcgaccatc atgtacgagg gtctgcccac gcgtcccgac ggcggcatct
    30841 ggtggcagct ggccgagaaa tacaaggtca cggtgatgtt cagcgcgccg accgctatcc
    30901 gcgtgctgaa aaagcaggac cccgaatacc tcaccaaata cgacctcagc aagctgcgcg
    30961 cgctgttcct ggccggcgag ccgctggacg agccgacggc gcagtggatc agcgagggcc
    31021 tcaagggcaa gcccatcatc gacaactact ggcagaccga gaccggctgg cccattctga
    31081 ccatctgcaa tggggttgaa aaggcgccgt cgaaattcgg ctcgccgggc aaggcggtct
    31141 acggttacga cgtcaagctg ctcgacgacc agaccggcga agagctgacc ggccccgacc
    31201 agaagggcgt gctggccatt gaaggcccgc tgccgcccgg ctgcctgcag accgtgtggc
    31261 gcgacgatgc gcgctttgtc aacacctact ggtcgagcgt gcccaacaag ctggtctaca
    31321 gcaccttcga ctgggccatc cgcgaccagg acggctacta cttcatcctg ggccgcaccg
    31381 acgacgtcat caacgtcgcc ggccaccgct tgggcacgcg cgagatcgaa gagagcattg
    31441 ccgcccaccc caacatcgcc gaagtagcgg tggtcggcgt ggccgatcag ctcaagggcc
    31501 aggtggccat ggcctttgcc gtgctgaaag atgccagcag cctgggcgat gaggcggcca
    31561 tgctcaagct ggaaggcgag gtgatgaagc tcgtcgacgg ttcactcggc gccgtggcgc
    31621 gcccggcgcg cgtgcgcttt gtgtcagtgc tgcccaagac gcgctccggc aagctgctgc
    31681 gccgcgccgt gcaggcggtg tgcgaggggc gcgacccggg cgacctgacc accatggaag
    31741 acccggcggc gctgcagcag atcaaggacc tggtcgcgcc cgcttgatcg cgctgggcga
    31801 tccgcccggc catcgcgccc ggcactgacc gagcgcctga cacgtgcttg tcagcgttcg
    31861 gtggcacaat gtgatttgcg aagttcccgg cccttccaca gccagtcgcg tccgccaacc
    31921 ggttcagccg tgccgcggaa ggtcttatcc accagcttga gtctccttca gggggacgaa
    31981 aggtcagcgt acccatgagc gagacgaaat ccgtctatca aacctaccag ggcaacacct
    32041 acctctttgg cggcaatgcg ccgtacgtgg aggagatgta tgaaagctac ctggccaatc
    32101 ccggcagcgt gcccgattcg tggcgcgaat atttcgatgc gctgcagcat gtgcccgcca
    32161 ccgatggcag caacgccaag gacgtgccgc acctgcccgt catcaacgcc tttgccgagc
    32221 gcgccaaggc cggcggcacg caggtggtgc aggccagcgg cgccgattcc gaactgggcc
    32281 gcaagcgcac cgccgtccag cagctgattg ccgcctaccg caacgtcggc gcccgctggg
    32341 ccgatctgga cccgctcaag cgtgccgagc gccccaacat ccctgagctg gagccgtcct
    32401 tctatggctt caccgatgcc gacatggaga cggtgttcaa caccagcaac accttcttcg
    32461 gcaaggacac catgcccttg cgcgagctgc tgaacgcgct gcgcgaaacc tactgcggca
    32521 ccatcggcgc cgagtacatg tacatcaccg accagggcca gaagcgctgg tggcagcaaa
    32581 aactcgaaag cgcgcgcgcc aacccgcaat tgagcgccga gcagaaaaag cacgtgctcg
    32641 agcgcctgac cgccgccgaa gggctggagc gctttctgca caccaagtac gtcggccaga
    32701 agcgcttctc gcttgaaggc ggcgagagct tcatcgtggc gatggatgag ctggtcaacc
    32761 aggccggcaa gcagggcgtg caggaaatcg tcatcggcat ggcgcaccgc gggcgcctca
    32821 acgtgctggt caacacgctg ggcaagatgc ccggcatgct gtttgccgag ttcgaccata
    32881 ccgcgcccga agaactgccg gccggcgacg tcaagtacca ccaaggcttt tcatccgacg
    32941 tgaccaccgc cggcggcccg gtgcacctga gcctggcctt caacccctcg cacctcgaga
    33001 tcgtcaaccc ggtggtcgaa ggcagcgtgc gcgcccgcct ggaccgccgc ggcgacaccg
    33061 aaggcgacac cgtgctgccc gtgctggtgc acggcgacgc cgcctttgcc ggccagggcg
    33121 tggtgatgga gacgctggcg ctggctgaaa cgcgcggcta ctacaccggc ggcacggtgc
    33181 acatcgtcat caacaaccag atcggcttca ccaccagcga cccgcgcgac agccgctcca
    33241 cgctgtactg caccgacatc gtgaagatga tcgacgcccc cgtgctgcac gtgaatggcg
    33301 acgaccccga agccgtggtg ctgtgcacgc agctggcgtt gcagtaccgg caggacttca
    33361 acaaggacgt ggtggtcgac atcgtgtgtt tccgcaagct cggccacaac gagcaggaca
    33421 cgcccatgct gacgcagccg ctgatgtaca ccaagattgg caagcacccc ggcacgcgca
    33481 agctgtacgg cgaaaagctg gtcacgcaag gcgtgctgcc ggccgacggc cccgaccaga
    33541 tggtggccac ctaccgcgcc gccatggacg ccggcaagca cacggtcgac ccggtgctga
    33601 ccaacttcaa gagcaagtac gcggtggatt ggtcgccctt cctgaaccag aagtggaccg
    33661 acgccgccga caccgccatc c
//
LOCUS       DAOMVO010000002        27471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_256_length_27471_cov_12.380617, whole genome shotgun sequence.
ACCESSION   DAOMVO010000002 DAOMVO010000000
VERSION     DAOMVO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 27471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27471
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_256_length_27471_cov_12.380617"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKV30_00195"
     CDS             <1..843
                     /locus_tag="PKV30_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL28671.1"
                     /translation="VETIGNPAGNITDIASVAAIAHAAGVPLIVDNTVATPFLIRPIE
                     HGADIVIHSLTKYMGGHGTTLGGVIVDSGKFPWAQHKARFKRLNEPDVSYHGVVYTEA
                     LGPAAYIGRARVVPLRNTGAALSPFNAWQILQGIETLALRMERICDNTLKVAQHLQKH
                     PKVAWVSYAGLPDHEDHALAQQYMKGRASGLFTFGVKSSTGDDARAAGARFLDALQLF
                     TRLVNIGDTKSLATHPASTTHRQLSPDELAKTGVKEETVRLCVGIEHIDDLLADLEQA
                     LGKV"
     gene            911..1126
                     /locus_tag="PKV30_00200"
     CDS             911..1126
                     /locus_tag="PKV30_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRL28672.1"
                     /translation="MRTTVTLDEALLGRAGALSGLQDRSTLLAEALRALIERESARRL
                     ARLGGSEPQLEAAPRRREAPPAKPTPA"
     gene            1123..1497
                     /locus_tag="PKV30_00205"
     CDS             1123..1497
                     /locus_tag="PKV30_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL28673.1"
                     /translation="MILADTSIWIDHLRGGDAVMTEQLERGSIAMHPFVLGEIALGSL
                     KSRAELLQLLEALPGTLVASDAEVMHMVDERALFGRGLGWVDVHLLAATLLTPGLRLW
                     TRDRRLHAVAEEIGLASSPGVH"
     gene            complement(1587..2027)
                     /locus_tag="PKV30_00210"
     CDS             complement(1587..2027)
                     /locus_tag="PKV30_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL28674.1"
                     /translation="MSLPTTLLLDTKVIWMLMRDPHGHVTEGLNRARALAPDLRVVTS
                     VVVDGELRFGLERRGSAKLQGAYAKVMQIIEVLPLPPEAAQPYATLRAHLESRGQPIG
                     PNDALIAAHALALGATLVSGDDEFARVPGLKVENWLKPLDVPGV"
     gene            complement(2024..2314)
                     /locus_tag="PKV30_00215"
     CDS             complement(2024..2314)
                     /locus_tag="PKV30_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein VapB"
                     /protein_id="HRL28675.1"
                     /translation="MSALPQYVSPAPNTRRVRFFRNGANQAVRIPREFELPGEEAVIE
                     KLGDTLVIRPLAAPLIKGSAASLAAVLATMEPIDEEFPDVDAGLLPLDDIEL"
     gene            2412..2768
                     /locus_tag="PKV30_00220"
     CDS             2412..2768
                     /locus_tag="PKV30_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRL28676.1"
                     /translation="MGWFSKSSGDFFLSSVVPQGQGGAQVAQFLGLVNGEAVIGANIF
                     RDMFSSVRDVVGGRAGGYERALAGAREAALDEAKEAAKAMGANGIVGIDFDYEVLGEA
                     NGMMLVSVTGTAVKMA"
     gene            complement(3110..3688)
                     /locus_tag="PKV30_00225"
     CDS             complement(3110..3688)
                     /locus_tag="PKV30_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28677.1"
                     /translation="MSTRSPALAAGREAEDDRPAIAPFWHRLNSFFLFPFQMEPLIYA
                     ALLALASYLLFLPGIIKFFTVLGLVLGASRYAFKVAALASRGVLHSRDYTNALTDPDW
                     KVLPWKFFGVLVGHGMLIGFLGRQSEVMGIVASLISSLLLPATLMVLIQSFSLRAALS
                     PFELLATVTAIGAQYSAISARWRSADLPLWRA"
     gene            3687..3986
                     /locus_tag="PKV30_00230"
     CDS             3687..3986
                     /locus_tag="PKV30_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28678.1"
                     /translation="MSGVHPQGRWANGAIVAVLGCCFFEMRTLGLNQGVCAARGRRGR
                     NIERKIASALVKRAPAAMVFEFQCVAHSPRTSSKAVELDLQRFARVGGTFFGAQR"
     gene            4177..6948
                     /locus_tag="PKV30_00235"
     CDS             4177..6948
                     /locus_tag="PKV30_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HRL28679.1"
                     /translation="MLTEFALGQIMRRAAPARLAQYLNPLNEAMAEFGIDTYLRAAAF
                     LAQIGHESGELVWLQERWGPTEAQKRCERPSRLAQRLGNTQPGDGARFRGRGPIQITG
                     RANYLRYGTLLGIDLVNAPERAAQPDVGFRIAGMYWQRNGLNDLADLGEFDQITQRID
                     GGQNGQADRQRLYALALSVLSPSDFPARAGAMAAPAAVEKEPLSRGWELLKEAAPPVR
                     AATRGTKRGSSAASARGPAARRFDARRDTMDFRDQMFVPTLVEVPPHVPLGEYVECEV
                     PILDQGQEGACTGYGLATVVHYLLRRRRVMPDHDGVSPRMLYEMARRYDEWPGQDYEG
                     SSARGAMKGWHKHGVCGDTDWPSDAPDGGLNEARVGAARRRPLGAYFRVNHRDLVAMH
                     AAIAEVGILYATATVHSGWEKVDAEGTIPLETTPLGGHAFAIVAYDTQGFWIQNSWGP
                     DWGLRGFAHISYDDWLSNGTDVWVARLGAPVELRKLASTAALQSGRSSQAIGYAYEDL
                     RPHVISVGNGGWLSPGDTYGTSESDVRRLFEQDIPRVMTNWPSKRIVLYVHGGLVPAA
                     DALQRVAEYRPALLAQQCYLLAFVWHSDFGSTLRNILADAVSKRRSGGWLDEMKDFLL
                     DRLDDMLEPLARQLLGKLAWDEMKENAVGASQKGRAADVVVHGLVKLKQRYPELELHL
                     VGHSAGSIFLGPLLARLGDAGLTARTCTLWAPACTYQDFEDFYQPALTARRGGAKALE
                     QLTLFMLTDAAEQDDNCALIYNKSLLYLVAHALERQSRIPGDPRHPGVPLLGMAKFLD
                     PAHPDYKADFKKRLDACGVECISAPNDDGEGTLTASKASRHGDFDNDKRTVLATFSRI
                     LGSASQLGAESVRDPMTGGQTTRVQVSNPDMLVGDEMVTLKLGESRSAASARRQVVDR
                     LSRPVTTAN"
     gene            complement(6994..7218)
                     /locus_tag="PKV30_00240"
     CDS             complement(6994..7218)
                     /locus_tag="PKV30_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HRL28680.1"
                     /translation="MPLNTEPNFSEAGRVYFQAYSPGDDFYELLIETHRDLSDEQSAA
                     VNARLILLLANHIGDIAVLREAMQIAREGS"
     gene            complement(7231..8898)
                     /locus_tag="PKV30_00245"
     CDS             complement(7231..8898)
                     /locus_tag="PKV30_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL28681.1"
                     /translation="MQKTFTYPKFDYVRPPELAEGRDGHYPVIVVGAGPVGLTAAIDL
                     AHQGQPVLLLDDDDTVSIGSRGVCYAKRTLEVLDRIGCDNRFVDKGVSWDVGRTFFGE
                     GEVFNFNLRPQAQHLRPGMINLQQYYLEQYLIERTGDVPQLEKRFKHKVIAVTPGDDK
                     VTLRVETPDGAYTLTTDWLVVADGARSPIRTMLGLDIEGKVFMDRFLIADVVMAADFP
                     AERWFWFDPPFHRNQSVLLHKQADNVWRIDFQLGWQADPVEEKKPENVIPRIKAMLGD
                     EREFELEWVSVYTFQCRRMNQFNHGRVLFVGDAAHQVSPFGARGANSGIQDTDNLAWK
                     LKLVMDSKAPPTLLDTYGEERGYAADENLMNSTRSTDFITPKSTTSKVFRNAVLQLAA
                     KYPFARALVNSGRLSVPSWLATSSLNTPDGDAFEGQMIPGAPLADAPVEVAGQPGWLL
                     HQLGNRFQLLHYVDDASTLDEKTALALAQLAQAAIAIEAIIVAPRGQAPAGLKTLIDT
                     DGCIAQRYDLRPGTTYLVRPDQHVAARWRALDASKIKAALARATCNA"
     gene            complement(9114..10076)
                     /locus_tag="PKV30_00250"
     CDS             complement(9114..10076)
                     /locus_tag="PKV30_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL28682.1"
                     /translation="MTEKKFASQADLEEKKISWEKLSDNAYAYTAEGDPNTGVIIGDD
                     GCMIIDATATPVAAQGVISKIREITDKPIKYVVLTHYHAVRVLGASGYKSEGLEQIIA
                     SQDTYDLIVERGQQDMDSEIGRFPRLFQAVESVPGLTWPTMTFTGEMTLWLGKLEVKI
                     KQIGRGHTKGDTIVYLPQQNICFSGDLVEADAACYTGDAYLADWPATLDALAAMKFDK
                     LVPGRGAALLTPDAVKKGLAYTRDFVSTLYTSAQEAVAQGMDLNATMKHTRKAMDPKF
                     AQVFIYEHCLPFDVTRAHDEASGVRDPRIWTAKRDQEMWHELQK"
     gene            10436..10585
                     /locus_tag="PKV30_00255"
     CDS             10436..10585
                     /locus_tag="PKV30_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28683.1"
                     /translation="MRIVEIDTATHSGVSYVVRPARYTTAGTVMVDAPPREYEVTFDG
                     MGFVR"
     gene            complement(10603..11457)
                     /locus_tag="PKV30_00260"
     CDS             complement(10603..11457)
                     /locus_tag="PKV30_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRL28684.1"
                     /translation="MLDESDETDGGPPPGDEGNGAAPRRQRGIQSIEVGLRVLQALTE
                     QRRPLPLKDIGRLADMAPAKAHPYLVSFMQGGLVKQNPLTGHYELGPGALQLGLAALQ
                     QLDPLTEASQEAAQLAAASDLSIALAVWGQLGPTVVRLDEPRYPLHVNLRVGTVMSLF
                     NTITGRVFAAYLPEKMVRSMLEDEHRRVVGGTSASFDAPEVQALLGEIRATGMGRGVS
                     MPQPGVNTLCAPVFDAAGHIALVMTMIGPQGVFDAEIDSAAGELLRQHARRTSLRLGH
                     SVLTTAIG"
     gene            11572..12123
                     /locus_tag="PKV30_00265"
     CDS             11572..12123
                     /locus_tag="PKV30_00265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL28685.1"
                     /translation="MSALRIAVVVGSIRKDSYNRRFAEALARLAPAGVEFNFLRIDDL
                     PHYNQDDDNDNAPEVKRFKAEVEAADGVLFVTPEYNRSFSGVLKNAIDQASRPYGQSK
                     WKGKPAAIVSVSPSPLSASMAAQHLRNVVAVLGMPTLNLPEMYIQWKDGLVDANGDIG
                     PASREFAQTFVNSFVDWVRRFKQ"
     gene            12168..12716
                     /locus_tag="PKV30_00270"
     CDS             12168..12716
                     /locus_tag="PKV30_00270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HRL28686.1"
                     /translation="MSQLTVAVVVGSIRKDSYNRQFAEALVKQLPADFKAQFIRIDDL
                     PLYNQDDDDNPAPQVTRLREEIKAADAVLFATAEYNRSIPGVLKNAIDHASRPWGKSV
                     WGGKPVGVVGVSVGAAGTCMAQQHLRNVISYLNMPQFGQPEMFIQWKEGLVKDGAIGP
                     DSKDFVQKYVDTFAAWVKKQVG"
     gene            12933..13454
                     /locus_tag="PKV30_00275"
     CDS             12933..13454
                     /locus_tag="PKV30_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRL28687.1"
                     /translation="MLDTFFARPRRVFGAIAVICAAMLAFGILYLQNVVGLEPCPMCI
                     VQRYALIIVGVFCAIAALSKSPASWRLWGLMAVAMSSFGAFTAARQSWLQWNPPPMAT
                     CGRDLFGMIESFPLQRAIPMIFKGSGDCSAVDWTFLGGTIANWSFVWFVIFGVVILAA
                     LLKLPRRGGVMPA"
     gene            complement(13479..13724)
                     /locus_tag="PKV30_00280"
     CDS             complement(13479..13724)
                     /locus_tag="PKV30_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRL28688.1"
                     /translation="MEIIWFLIVGLVAGWLAGVLVKGGGYGLVGDMVVGILGALLGGY
                     LFGSLGGAVGGGLLGSIVVATLGAVILIVILRLLKRA"
     gene            complement(13846..15192)
                     /gene="argA"
                     /locus_tag="PKV30_00285"
     CDS             complement(13846..15192)
                     /gene="argA"
                     /locus_tag="PKV30_00285"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HRL28689.1"
                     /translation="MSTVFNFTFVPWFRAVAPYIHMHRGKTFVVGVVGEAIAAGKLAS
                     IATDLALIQSMGVKIVLVHGFRPQVNEQLRAKGHEPRYAHGLRITDEVALDSAQEAAG
                     QLRFEIEAAFSQGLPNTPMAGSKVRVISGNFLTARPMGIIDGVDFKHTGLVRKVDVAG
                     ITRSLDAGALVLISPFGFSPTGEAFNLAMEEVATSVAMALQADKLIFLTEKPGIRVHP
                     LEPESDDNPIDTEIPLAEARALIQRLPLPEEPMDVGFYLKHCVTACEHGVERSHILPY
                     AVEGALLLEVYVHDGIGTMVVDEKLESLREATPDDVAGILQLIEPFEKDGTLVKRSRT
                     EIERDIGNYTIVEHDGVIFACAALYPYPEARTGEMAAVTVSPQSQGQGDGEKILKRIE
                     QRARAMGLESIFVLTTRTMHWFIKRGFAQVDPEWLPEARKRKYNWDRKSQVLVKKL"
     gene            15484..15945
                     /locus_tag="PKV30_00290"
     CDS             15484..15945
                     /locus_tag="PKV30_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRL28690.1"
                     /translation="MKIELTVNGRPVAADVPENTLLVQLLRDTLQLTGTHVGCDTAQC
                     GACTVIVGDRAIKSCNVLAAQLQGAEVTTIEGVAHADGSMHPVQAAFKDCHGLQCGFC
                     STGMVMSSIDLLKHHPKASEAEIRELLEGNICRCTGYQNIVKAVQQAQAAL"
     gene            15958..>18321
                     /locus_tag="PKV30_00295"
     CDS             15958..>18321
                     /locus_tag="PKV30_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRL28691.1"
                     /translation="MGANDFAKLPHIGESMKRKEDERFLTGNGQYTDDITQAKQKYAV
                     FVRSPHAHAVIKSVDIVEASEMPGVVKIFTGKDMEGKMGGLPCGWLINNPDGSPMKEP
                     PHPILAHSKVRYVGDHVAMVVADTLQQAKDAAEKVEVDYEVLGPVIDMRTAKNGPALH
                     DEAPDNHCYKWTLGDKAGVDEAFAKAAHVTKLDITNNRLIPNAMEPRAAIASYNRATE
                     EYELYTTSQNPHVARLLMTAFVLSLPEHKVRVIAPDVGGGFGSKIFLYAEEVACTWAA
                     KQLNASIKWTAERSESFVSDAHGRDHITHAEMAMDKDGKFLAMRVHTDANLGAYLSTF
                     ASSVPTILYGTLLAGQYTAPQIYVEVDGWFTSTAPVDAYRGAGRPEATYVVERLVTRC
                     AWEMGLSQDEIRKRNFITSFPYQTPVALQYDIGDYNACMNDAEKLADVAGFEVRRTAS
                     EAKGLKRGIGYSSYIEACGLAPSNIAGALGARAGLFEAGEVRVHPTGSVTVFTGSHSH
                     GQGHETTFAQLVAARLGIDPSQVDIVHGDTGRVPFGMGTYGSRSLSVGGTAIMKALDK
                     IEAKAKKIAAHLMEASDADIEFSGGEFKVKGTDKKIPFGQVALTAYVPHNYPLDKLEP
                     GLNETAFYDPTNFTFPAGTYICEVEVDPATGVVRVDRFTAVDDFGVIINPMIVEGQVH
                     GGLVQGIGQALMEHGVYDPESGQLLTGSYMDYTMPRAGDFPEFKLGHVCTPCTHNPIG
                     SKGCGEAGAIGSPPAVINAVLDALKPLGVKDLDMPATPHRVWQAIQNA"
     assembly_gap    18322..18421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18422..18988
                     /locus_tag="PKV30_00300"
     CDS             <18422..18988
                     /locus_tag="PKV30_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRL28692.1"
                     /translation="VANNADVKAMIPALASLAEGIGDKQVRAMGTIGGSLANNDPAAD
                     YPAAVLALGATVITTEREIAADDYFQGMFATVLEEGELITAVRFPKPQRSAYMKFRQP
                     ASLFALIGVFVAQTPDGARVAVTGGGNGVFRQTAMEQALTKSFTPEAIADIRTDASEI
                     NGDLHGSADYRAHLVGVMAQRAVAKAVA"
     gene            complement(19091..19567)
                     /locus_tag="PKV30_00305"
     CDS             complement(19091..19567)
                     /locus_tag="PKV30_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28693.1"
                     /translation="MVDAVMYLLRTLIGRSHARTLARQVRAASPEAGRGVLAQALPEE
                     LRPLLDTKQLEAMRQRLLALPPQHGMALGRTDFYPAIAIFWMVVLATFPVVLPIVWMD
                     DATMALHTSRGITLVMLFLCGPAFARYAGHPHPMRIGLVMAALTTLLSLAVMALGG"
     gene            complement(19583..19777)
                     /locus_tag="PKV30_00310"
     CDS             complement(19583..19777)
                     /locus_tag="PKV30_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28694.1"
                     /translation="MSAEALPMTPQNQPAVVREQLLNPVDRISEVLFGLIMAVTIIGS
                     LSIASAGPLEALTLRAAALG"
     gene            19846..20748
                     /locus_tag="PKV30_00315"
     CDS             19846..20748
                     /locus_tag="PKV30_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL28695.1"
                     /translation="MNALASIDGLQQELQDAGYFADRRLATAAFLALKLQRPLLLEGE
                     PGVGKTELAKALAQTLGRELLRLQCYDGLEQREAMYEWNYAAQLLHLRAVEGKAPAEE
                     IEREVYQPHYLIRRPLLQALQAPAPGAVLLIDEVDRADEPFEAFLLEYLGEYQVSIPE
                     LGTVRAVCTPVTILTSNRTRELNDAVKRRCLYHWLDYPERERELAIVRARAPDAPQAL
                     AEQVARFVQRLRSAPYAGHFQRAPGIAESVEWAKALVALDTLHLDPEVVQGTAGVLFK
                     QREDVAALTEPLVAELLEAQAVDP"
     gene            20866..22059
                     /locus_tag="PKV30_00320"
     CDS             20866..22059
                     /locus_tag="PKV30_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRL28696.1"
                     /translation="MQLGDARTGKLAGNIAAFSRTLRRAGVPVDASRIVLAQQALQCV
                     ELDRKPDVAAALEAVLVSREGDRAVFRELFDAFFRDPELANKLLAQMLPRAEGQAERP
                     RQRPRVREALQPQRAAQMLKPAEPDKEIDLDAAMTASDAERLRQADFNQLTASEYRLV
                     ERLVRDIRLPVPAVPSRRTRAGWRGHRVHWGHTLQGAMHTGGEVMALHRLQRRVQPLP
                     LLVLVDVSGSMERYARLLLAFLHAATRQAHGMRIRRDVFAFGTHLTDLTPAFRLQDAD
                     AMLTEASRAIDDFAGGTRLGDSLAALRRRHARRLVGRRTLVLIVSDGLDTGEPEELAR
                     ELACLKRHTRRLLWLNPLLRFDGYAPTARGAAELHRAADGMLAVHNLSALQTLGHAIA
                     ALIQQ"
     gene            22079..22621
                     /locus_tag="PKV30_00325"
     CDS             22079..22621
                     /locus_tag="PKV30_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRL28697.1"
                     /translation="MDMQGSRQLAVTQQQAWEALNDPEVLKVCIPGCDSIEATGEGAY
                     ALTNAIKVGPVSAKFKGNLQLTDIQAPDSYTINFEGNGGAAGFGKGSAKVNLVPNDAG
                     CELGYTVNASVGGKIAQVGQRLIDGVAKSMAESFFKRFDEEMNRRHGPPPDEATAEEP
                     GKLKRMWGKLTGSSKAEDAG"
     gene            22640..23686
                     /locus_tag="PKV30_00330"
     CDS             22640..23686
                     /locus_tag="PKV30_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRL28698.1"
                     /translation="MENLDTAVLRALRDWRAAGQQALLVTVVRTWGSSPRPVGSIMAL
                     KKNGSVAGSVSGGCIEDDLIARYARRGAADALPHSGKPQFAKYGITADEAHRFGLPCG
                     GTLELLFEFDPDAASLTELVRALDAGRLMRRTVHLADGRVTLAPADAPTELNVDDAQL
                     TNTFGPEYRMLIIGAGQMSEYLATMAQFCGFAVTVCDPREEYRGGWSVPGVHLATDMP
                     DDVVTAFKPDKRSCVIALTHDPKLDDLALLEALGSEAFYIGALGSRRNNTARRQRLIE
                     HFDQTEASLAKLRGPVGIFIGSKTPPEIAASVMAEVLAVKNGVTLPRDLEVAHVKDQQ
                     AAQPGRTASSCAMG"
     gene            23700..24365
                     /locus_tag="PKV30_00335"
     CDS             23700..24365
                     /locus_tag="PKV30_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRL28699.1"
                     /translation="MALDAIQDIAGIVLAAGAGRRMGYRPKALLQRDGQPLVARQIGL
                     LAQCSVRRIMVVLGHHAARIAPVLATTATALPGVDLIWVVNPTPDEGTGASLRCALAA
                     LPADLTGVMVLLADQPLLQVDDVACVLAAWRARPADIDLIVPTHEGQPGHPLIFGPQV
                     RQGVANGQGAAGVRDWRRAHPRQVLALPVDHARCTQDIDTEDDLARLTAEQGVRLSWP
                     DVT"
     gene            complement(24437..>24652)
                     /locus_tag="PKV30_00340"
     CDS             complement(24437..>24652)
                     /locus_tag="PKV30_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28700.1"
                     /translation="LLAAELARAATLLQEHGTAFRQLVRQLLADGAEPAATFGHVTGL
                     AVRVDDAQGDANGPEPWHARWEAFAGN"
     assembly_gap    24653..24752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24753..24993)
                     /locus_tag="PKV30_00345"
     CDS             complement(<24753..24993)
                     /locus_tag="PKV30_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28701.1"
                     /translation="MDLHDTRFLQREPLNQRLAERKAQLAAIAAELKTELLGIDEVID
                     RVIEAVRAWYVLPEIIKRPVIFCQWGLTGTGKTQLT"
     gene            complement(24995..25237)
                     /locus_tag="PKV30_00350"
     CDS             complement(24995..25237)
                     /locus_tag="PKV30_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28702.1"
                     /translation="MSAKTFSRAAAGGAQANLKQLRRMRAPLSLQLPPPRNPIALAMA
                     TRSVSSGAGKHVRSQGAQRRADRVALQKAVRNAWND"
     gene            25701..26672
                     /locus_tag="PKV30_00355"
     CDS             25701..26672
                     /locus_tag="PKV30_00355"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRL28703.1"
                     /translation="MRTRITDLLGIQYPIVQGGMHFVGLAELASAVSNAGGLGILTAL
                     TQPSPAALAEEIARCRAMTDKPFGVNLTFLPARTPPDYPGYVRTIVDGGIKVVETAGN
                     NPQKWMPMLKEAGIKVIHKCTSVRHALKAEAIGCDAVSVDGFECGGHPGEDDVPNFIL
                     LPRAAQELKIPFIASGGMADGRSLVAAMAMGAEGMNMGTRFIATKEAPVHEKVKQAIV
                     AASELDTRLVMRPLRNTERVLSNAGVERLLAKERALGDKITFEDIIAEVGGVYPRVMK
                     DGDLDAGAWSCGMVAGLIHDIPTVKELIDRIVAEAQQIVRERLAGMV"
     gene            complement(26771..27193)
                     /locus_tag="PKV30_00360"
     CDS             complement(26771..27193)
                     /locus_tag="PKV30_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRL28704.1"
                     /translation="MIRTVCTLAVVAALTACASAPDTAKLSAPTANSGLSGDTPVGLV
                     DLAAHKMAGTPADHDLRARALTLAPGGGVGNHPHAGRPGIVRVVKGTVVEGRGDAKRV
                     LKPGDFWLETFDTSHWFVNPSTTEAAELWVVDIVPKKK"
BASE COUNT         4760 a         8899 c         9106 g         4506 t
ORIGIN      
        1 gtcgaaacca tcggcaaccc ggccggcaac atcaccgaca tcgccagcgt ggccgccatc
       61 gcgcacgcgg cgggcgtgcc gctgatcgtc gacaacaccg tcgccacgcc gttcctgatt
      121 cgcccgatcg agcacggtgc cgacatcgtc atccactcgc tgaccaaata catgggcggc
      181 cacggcacca cgctgggcgg cgtcatcgtc gattcgggca agtttccgtg ggcgcagcac
      241 aaggcgcgct tcaagcgcct gaacgagcct gacgtgagct accacggcgt cgtctacacc
      301 gaagcgctgg gcccggcggc ctacatcggc cgcgcgcgcg tcgtgccgct gcgcaacacg
      361 ggcgcggcgc tgtcgccctt caacgcctgg caaatcctgc aaggcatcga gaccctggcg
      421 ctgcgcatgg agcgcatctg cgacaacacc ttgaaggtgg cgcagcacct gcaaaagcac
      481 cccaaggtcg cctgggtcag ctacgccggc ctgcccgacc acgaagatca tgcgctggcg
      541 cagcaataca tgaaagggcg cgcctcgggc ctgttcacct ttggcgtcaa atccagcacc
      601 ggcgacgacg cgcgggcggc cggcgcgcgt tttctggatg cgctgcaatt gttcacccgc
      661 ctggtcaaca tcggcgacac caagtcgctg gccacccacc cggccagcac cacgcaccgc
      721 cagctctcgc ccgacgagtt ggccaagacc ggcgtgaaag aagaaaccgt gcgcctgtgc
      781 gtgggcatcg agcacatcga cgatctgctg gcggatctgg agcaggcgtt ggggaaggtt
      841 tgacgtcgga gcctgcgttc gcatcaataa acgcataaaa tgatccgata attgatgaag
      901 gaagagcatc atgcgaacca cagtcaccct cgacgaagcc ttgctgggcc gcgctggcgc
      961 cttgagcggg ctgcaagacc gctcgacctt gttggccgag gcgctgcgcg ccttgatcga
     1021 acgcgaaagc gcacgccgct tggcgcggct gggcggcagc gagccgcagc ttgaagccgc
     1081 gccgcgtcgg cgcgaggcgc cgcctgccaa gccgacgccg gcatgatcct ggccgacacc
     1141 tccatctgga tcgatcattt gcgtggaggc gacgcagtga tgaccgagca gcttgagcgc
     1201 gggtcgattg ccatgcaccc gtttgtgctg ggggaaatcg cactgggctc gttgaaatca
     1261 cgtgccgaac tgctgcaact gcttgaagcg ctgcccggca cactcgtcgc gtccgatgcc
     1321 gaggtgatgc acatggttga tgagcgcgcc ttgtttgggc gtggcctggg ttgggtggat
     1381 gtgcacctgc tggccgccac gctgctgacg ccgggcctgc gcctttggac gcgggaccgg
     1441 cgcttgcatg ccgtcgccga ggaaatcggg ctcgcttcgt cgccaggcgt gcattgaact
     1501 cagccagact cggccaaatt gattcaataa agagagctgc ttgcgcttgc ctggccagcg
     1561 ctcgaggcct gaaagccttg taaaaatcaa acgcctggca cgtccagcgg cttgagccag
     1621 ttctccacct tcagccccgg cacgcgggcg aattcgtcgt cgccgctgac cagcgtggcg
     1681 cccagcgcca gcgcgtgggc ggcgatcagc gcgtcgttgg ggccgatggg ctggcctcgg
     1741 gattcgagat gggcgcgcag ggtggcgtag ggctgcgcgg cctcgggcgg caggggcaaa
     1801 acctcgatga tttgcatcac tttggcgtag gcgccttgca gcttggctga gcctcggcgt
     1861 tccaggccaa agcgcaactc gccatcgacc accacgctgg tgacgacgcg cagatcgggc
     1921 gccagcgctc tggcgcggtt cagaccttca gtcacgtggc cgtgcgggtc gcgcatcagc
     1981 atccagatga ctttggtgtc cagcagcaaa gtggtgggca agctcatagc tcgatgtcat
     2041 cgagcggcag caggcctgcg tccacgtcgg ggaattcttc gtcaatcggc tccatggtcg
     2101 ccagcacggc cgccagcgag gctgcgctgc ccttgatcag cggcgcagcc aacgggcgga
     2161 tgacgagggt gtcgccaagc ttctcgatga ccgcttcctc ccccggcaac tcaaactcgc
     2221 gcggaatgcg cacggcctgg ttggcgccgt tgcggaaaaa gcgcacgcgg cgggtgttgg
     2281 gggcggggga aacgtattgg ggcagggcgc tcatggcggg cctccgtatc gaaatcgcag
     2341 gtttgaagta tagcctatgc catcgcatag gctatactaa atcaccgttc atcaacgagg
     2401 taggtgcatt catgggctgg ttctccaaat cgtcaggtga tttcttcttg tcgagcgtgg
     2461 tgccccaagg ccagggcggc gcgcaggtgg cgcagtttct gggccttgtg aatggcgagg
     2521 cggtgattgg cgccaacatc tttcgcgaca tgttttccag cgtgcgcgac gtggtgggcg
     2581 gccgtgccgg cggctacgag cgggcgctgg ctggcgcgcg cgaggcggcg ctggatgagg
     2641 cgaaagaagc ggccaaggcg atgggcgcca acggcatcgt cggcatcgat ttcgactacg
     2701 aggtgctggg cgaggccaac ggcatgatgc tggtgtcggt cacgggcacg gcggtgaaga
     2761 tggcgtgatg cgcaggattc aagccatttc ggcctccagc gcttgccaga caagcgcaag
     2821 cagctatcaa aacaagagaa tctgatgcaa cgaccttgac ggcgctttaa gaccaaagca
     2881 cgcgttgcgc gacaatttgc ggcgccatgt tcatcgccgt tccgctcgaa accaatgcct
     2941 cctggcgctc gccgccgtgg atgacgctgc tgctgattgc gctccactgc ctggttttct
     3001 tcggctggca gctgcccgaa gagcgcgcgg tcaacaaggc cgccgcggct tacgccaaga
     3061 cgccgctggc cgccatcgag ctgccgcagt tcgtgcagca tctgcaaaat caagcgcgcc
     3121 aaagcggcaa atcggccgac cgccagcgcg ccgaaatggc cgagtattgc gcgccgatgg
     3181 cggtcacggt ggccagcagc tcgaacgggc tcagcgcagc gcgcaggctg aaggactgga
     3241 tcaacaccat cagcgtggcc ggcagcagca gcgacgagat caggctggcg acaatgccca
     3301 ttacctcgct ctgccggccc agaaaaccga tgagcatgcc gtgccccacc agcacgccaa
     3361 agaacttcca gggcagcacc ttccagtccg ggtcggtcag ggcgttggtg tagtcgcggc
     3421 tgtgcagcac gccgcgcgag gccaacgccg ccaccttgaa ggcatagcgg ctggcgccaa
     3481 gcaccaggcc cagcacggtg aagaacttga tgatgccggg cagaaacagc aggtagctgg
     3541 cgagcgccaa cagcgccgcg taaatcagcg gctccatctg aaatgggaat agaaagaagc
     3601 tgttcagccg gtgccagaag ggcgcgatgg cggggcgatc gtcttcggct tcacggccgg
     3661 cggcgagggc gggggagcgg gtagacatga gtggtgtgca tccacaaggc cgttgggcca
     3721 acggcgccat tgtcgcagtg ctgggctgct gttttttcga aatgcgtact ttgggcctca
     3781 atcaaggggt gtgtgcagcg cgcgggcggc gcggcagaaa catcgaacga aaaatcgcct
     3841 cagcgcttgt caagcgtgcc ccagcagcta tggtttttga gtttcagtgc gtggcccatt
     3901 cgccgcgcac atcatcaaaa gccgttgagc tggatctgca gcgattcgcg cgcgtgggcg
     3961 gcactttctt cggcgctcaa cgataaggac ggccgttcac gctggcgctg ggtgcgttca
     4021 gatcgacgcg cgggcaagac cagccagcgg ttgcatctgg tatcggccct cctacctcgc
     4081 cgaggggtcg ttcaggcgcg cggttcggcg ccagaatcgg ccgcgtgtcc acgcgcagcc
     4141 atggcgtgca gtaggcgatc acgcaggagg tgtcacatgc tgaccgagtt tgctctgggg
     4201 cagatcatgc gccgcgccgc gccggcccga ttggcgcagt acctgaatcc gctgaacgag
     4261 gcgatggccg agttcggcat cgacacctat ttgcgggcag cggcctttct ggcgcagatc
     4321 ggccacgaat ctggcgaact ggtgtggctg caggaacgct gggggcccac cgaggcgcaa
     4381 aagcgctgcg aacgcccgag cagactggct cagcggctgg gcaacaccca gcccggcgat
     4441 ggcgcccgct tcaggggccg tggcccgatc cagatcaccg gcagggccaa ctaccttcgc
     4501 tacggcacac tgctcgggat cgacctggtc aacgcccccg agcgcgccgc ccagcccgat
     4561 gtgggctttc gcatcgccgg gatgtactgg cagcgcaatg gtctgaacga cttggccgac
     4621 ctgggtgagt tcgaccagat aacccagcgc atcgatggcg gccagaacgg acaggccgac
     4681 cgtcagcggc tgtacgcgct ggcgctcagt gtgctgagtc cgtcggattt tccagcgcgc
     4741 gctggcgcga tggcggcgcc ggccgcagtc gaaaaggagc cgctcagccg cggatgggag
     4801 ctgctgaaag aggccgcgcc gcccgttcgc gccgccacca ggggcacaaa gcgcggcagc
     4861 agcgctgcct cggcgcgggg ccctgcggca cgtcgcttcg atgcgcgccg cgacacgatg
     4921 gattttcgcg atcagatgtt cgtgcccacg ctggtcgaag tgccgccgca tgtgccactg
     4981 ggcgaatacg tggagtgcga ggttccgatc ctcgatcaag gccaggaagg tgcttgcacc
     5041 ggttatgggc tggccacggt ggtgcattac ctgctgcgcc gccgccgcgt catgcccgac
     5101 cacgacggcg tcagcccgcg catgctgtat gaaatggcgc gccgctacga cgaatggccg
     5161 ggacaggact acgaaggttc aagcgcgcgc ggcgccatga agggctggca caagcacggc
     5221 gtgtgcggcg acaccgactg gccgtccgac gcgcccgacg gcggtttgaa cgaagcgcgc
     5281 gtcggcgccg ccagaaggcg gccgctgggc gcgtacttcc gcgtcaatca ccgtgatctg
     5341 gtggccatgc acgcggcgat cgccgaagtg ggcatcctgt acgccacggc caccgtgcac
     5401 agcggctggg agaaggtcga cgccgaaggg acgattccgc tggagaccac gccgctgggc
     5461 ggccatgcct tcgccatcgt cgcctacgac acgcaaggtt tctggatcca gaattcctgg
     5521 ggccccgact ggggcctgcg cggctttgcc cacatcagct acgacgactg gctgtccaac
     5581 ggcaccgacg tgtgggtggc gcgcctgggc gctccggtag aactgcgcaa gctggcgtcg
     5641 actgcggcgc tgcaatcggg gcgctccagc caggccatcg gctacgccta cgaagacctg
     5701 cgcccgcacg tgatcagcgt cggcaacggc ggctggctca gccccggcga cacctacggc
     5761 accagcgaga gcgacgtgcg ccgcctgttc gagcaggaca ttccgcgcgt gatgaccaat
     5821 tggccatcca agcgcatcgt gctgtacgtg catggcggcc tggtgcccgc cgccgacgcc
     5881 ttgcagcggg tggccgaata ccggcctgcg ctgctggcgc agcagtgcta tctgctggcc
     5941 ttcgtctggc acagcgactt cggctcgacg ctgcgcaaca tcctggccga tgcggtgagc
     6001 aagcgccgca gcggcggctg gctcgacgag atgaaagact tcctgctgga ccggcttgac
     6061 gacatgctgg agccgctggc ccgccaactg ctgggcaagc tggcatggga cgaaatgaag
     6121 gaaaacgccg tcggcgcaag ccagaagggg cgcgcggcgg acgtggtggt gcacggcctc
     6181 gtcaagctca agcagcgtta ccccgagctc gaactgcatc tggtcggcca cagcgctggc
     6241 tcgatttttc tcggcccgct gctggcgcgg ctgggtgatg cgggtttgac ggcacgcacc
     6301 tgcacgctgt gggcgcccgc ctgcacgtac caggatttcg aggacttcta ccagcccgcg
     6361 ctcaccgcgc gccgaggcgg cgcgaaagcg ctcgagcagc tgacgctgtt catgctgacc
     6421 gatgcggctg agcaggacga caactgcgcg ctgatctaca acaaatcgct gctgtacctg
     6481 gtggcccatg cgctggagcg gcaatcgcgc atcccgggcg atccccgcca tccaggcgtg
     6541 ccgctgctgg gcatggcgaa atttctcgac ccggcccacc cggactacaa ggccgacttc
     6601 aagaaacggc tggacgcctg cggtgtcgag tgcatcagcg cgcccaacga cgacggcgaa
     6661 ggcacgctga ccgcctccaa ggcctcgcgc cacggcgatt tcgacaacga caagcgcacc
     6721 gtgctggcga ccttcagccg catcctgggc tccgccagcc aactgggggc tgaatcggtg
     6781 cgtgatccga tgaccggcgg ccagaccacg cgggtgcagg tgagcaaccc cgacatgctg
     6841 gtcggcgacg agatggtgac gctgaaactg ggcgaatcgc gcagcgcggc gtcggcgcgc
     6901 cgtcaggtgg tggaccggct ttcgcgcccg gtcacgaccg ccaactgacg agtgtctggc
     6961 gacggcgctg tcgcttgccg atgatcggcg gattcaagaa ccctcgcgtg cgatctgcat
     7021 cgcctcgcgc agcacggcga tgtcgccaat gtggttggcc agcagcagga tcaggcgcgc
     7081 attgacggcg gcgctttgct cgtcgctcag gtcgcggtgg gtttcgatca gcagttcgta
     7141 gaagtcgtcg cccggcgaat aggcctggaa gtagacgcgg ccggcttcgc tgaagttggg
     7201 ttcggtgttg aggggcatgg cgtgttttcc tcaggcgttg caggtggcgc gggccagggc
     7261 tgctttgatt ttggaagcat ccagcgcgcg ccagcgggcg gccacgtgct ggtcggggcg
     7321 cacaaggtag gtcgtgccgg ggcgcaggtc gtagcgctgg gcgatgcagc cgtcggtgtc
     7381 gatcagcgtc ttcaagccag cgggcgcttg gccgcgtggg gcgacgatga ttgcttcgat
     7441 agcaatagct gcttgcgcaa gctgggcaag cgctagagcc gttttttcat ccaaagtgct
     7501 ggcgtcatcg acgtagtgca gcaactgaaa gcgattgccc agctggtgca gcagccagcc
     7561 aggctggccc gccacttcaa ccggcgcatc ggccagcggc gcgccgggaa tcatctggcc
     7621 ctcaaacgca tcgccgtccg gcgtgttcag gctcgacgtc gccagccagc tcggcaccga
     7681 caggcgccct gaattgacca gcgcgcgagc aaacgggtac ttggccgcca gttgcagcac
     7741 ggcgttgcga aacaccttgc tggtggtgct cttgggggtg atgaagtcgg tcgaacgggt
     7801 cgagttcatc aggttttcgt cggccgcgta gccgcgctct tcgccgtagg tgtcgagcag
     7861 cgtgggcggc gccttgctgt ccatcaccag cttcagtttc caggccaggt tgtcggtgtc
     7921 ctggatgccg ctgttggcgc cgcgcgcgcc aaagggcgag acctggtgcg ccgcgtcgcc
     7981 cacgaacagc acgcgcccgt ggttgaactg gttcatgcgc cggcactgga aggtgtagac
     8041 gctgacccat tccagctcaa actcgcgctc atcgcccagc atggccttga tgcgggggat
     8101 gacgttttcg ggctttttct cttccaccgg gtcggcctgc cagccgagtt ggaagtcgat
     8161 gcgccagacg ttgtcggcct gcttgtgcag cagcaccgac tggttgcggt gaaacggcgg
     8221 gtcaaaccag aaccagcgct cggcggggaa gtccgccgcc atcaccacgt cggcgatgag
     8281 aaagcggtcc atgaagacct tgccttcgat gtccagcccc agcatggtgc ggatggggct
     8341 gcgcgcgcca tcggccacca ccagccagtc ggtcgtcagg gtgtaggcgc cgtcgggtgt
     8401 ttcgacgcgc agcgtgactt tgtcatcgcc cggcgtgacg gcaatcacct tgtgcttgaa
     8461 gcgtttctca agctgcggca cgtcgcccgt gcgctcgatc aggtactgct ccaggtagta
     8521 ctgctgcagg ttgatcatgc cggggcgcag gtgctgagcc tgcgggcgca ggttgaagtt
     8581 gaacacctcg ccctcgccaa agaaggtgcg acccacgtcc cagctcacgc ccttgtcgac
     8641 aaagcggttg tcgcagccga tgcggtcgag cacctccagc gtgcgcttgg cgtagcacac
     8701 gccgcgcgag ccgatggaga cggtgtcgtc gtcgtccagc agcagcacgg gttggccctg
     8761 gtgcgccagg tcgatggcgg cagtcaagcc cactggaccg gcgccaacga cgatcacggg
     8821 gtagtgccca tcgcgcccct cggccagctc gggcgggcgc acgtagtcga acttggggta
     8881 ggtgaaggtt ttttgcaccg cttgtctcct gagggtaggc ggtttatgaa tcaaatcagg
     8941 ctctggcgct tgaaggacaa gcgcgagcag ctatcaaaac aaaagcaacg gtctgaattc
     9001 aagaggccgc ggagcaggcc attccagcgg ccgcccgagc cggggttccc ccggcgacca
     9061 gcggcgtctc tcgtgcgaag cgaaggggga agcggcgtca gccgcccacg gggttacttc
     9121 tgcaattcat gccacatttc ttgatcgcgc tttgccgtcc agatgcgcgg atcgcgcacg
     9181 ccgctggcct cgtcgtgcgc gcgggtcacg tcaaacggca ggcaatgttc gtagatgaac
     9241 acttgcgcga acttcgggtc catcgccttg cgggtgtgct tcatggtggc gttcaggtcc
     9301 atgccttgcg ccaccgcttc ttgcgcgctg gtgtagaggg tggacacgaa atcgcgcgtg
     9361 taggccagcc cttttttcac cgcatcaggc gtcagcaacg ccgcgccgcg gccgggcacc
     9421 agcttgtcga acttcatggc ggccagcgca tccagcgtgg ccggccagtc ggccaggtag
     9481 gcgtcgcccg tgtagcaggc ggcgtcggcc tcgaccaggt cgcccgagaa acagatgttc
     9541 tgctgcggca ggtagacgat ggtgtcgccc ttggtgtggc cgcggccgat ctgcttgatc
     9601 ttgacctcca gcttgcccag ccacagcgtc atctcgccgg tgaaggtcat cgtcggccag
     9661 gtcaggccgg gcacgctttc gacggcctgg aacaggcgcg ggaagcggcc gatttcggag
     9721 tccatgtcct gctggccgcg ctcgacgatg aggtcatacg tgtcctggct ggcgatgatc
     9781 tgttcaagtc cttccgattt gtagcccgac gcgcccagca cgcgcaccgc gtggtagtgc
     9841 gtcagcacga cgtacttgat gggcttgtcg gtgatctcgc ggatcttgct gatcacgccc
     9901 tgcgcggcca ccggcgtggc ggtggcgtcg atgatcatgc agccgtcgtc gccaatgatg
     9961 acgccggtgt tcgggtcgcc ctcggcggtg taggcgtagg cgttgtctga gagtttttcc
    10021 cagctgatct tcttttcttc caggtcggct tgggaggcga atttcttttc ggtcatgcgc
    10081 ggtctctgtg ttcgtggggg ttgggggatt gtcgaaaaaa attcgatatc agtcaattga
    10141 ttcgacaata ttgaatttta tggatgtggg ccgatgatgc tgccgcacgg cggtgcaaca
    10201 gccgcttacg tgcccacggg tactgtccgg agattggcgc ggcgcttcag tcgccgcgat
    10261 ggtctgcccg cgcgcgcatc acccggccgg gggccggctc cgtcaaaaca tctctgaggg
    10321 actcacacat gcaatctcac acttggcgct ggttggcacc tgcggcggcg tgcttgttgg
    10381 cgctgccagc gcgtgcgctg gattgtgcgc agctggccac acccgtctgg cgcccgtgcg
    10441 catcgtcgag atcgacacgg ccacgcacag cggggtgagc tatgtcgtgc ggcccgcgcg
    10501 ctacaccacg gccggcaccg tgatggtcga tgcgccgccg cgcgaatacg aggtcacttt
    10561 cgacggcatg ggttttgtgc gctgacgcgc gcccgcgcgc agtcagccga tggcggttgt
    10621 caggacgctg tggccaagtc gcagcgacgt gcggcgcgcg tgctggcgca gcaattcccc
    10681 ggcagcgctg tcgatttccg catcgaacac cccctgcggc ccgatcatcg tcatcaccag
    10741 cgcgatgtgc ccggcggcat caaacaccgg cgcacacagc gtgttgacgc cgggctgcgg
    10801 catcgagaca ccgcgcccca tgccggtggc gcggatttcg cccagcagcg cctgcacctc
    10861 gggcgcatcg aacgaggccg aggtgccgcc gaccacgcgg cggtgctcgt cttccagcat
    10921 gctgcgcacc attttttcgg gcaggtaggc ggcaaacacg cggccggtga tggtgttgaa
    10981 gagcgacatg acggtgccca cgcgcaggtt cacgtgcagc gggtagcgcg gctcatccag
    11041 ccgcacaacg gtggggccga gctggcccca caccgccaag gcaatgctga ggtcggacgc
    11101 ggcggccagt tgcgccgctt cctggctggc ctcggtcagc ggatcgagct gctgcagcgc
    11161 ggccaggccc agttgcaggg cgccggggcc gagctcgtaa tggcccgtga gcgggttctg
    11221 cttgaccagc ccgccctgca tgaagctcac cagatacggg tgcgccttgg ccggcgccat
    11281 gtcggccagg cggccgatgt ctttcagcgg cagcgggcgg cgctgctcgg tcaatgcctg
    11341 cagcacgcgc aggccgacct cgatcgactg gatgccgcgc tggcgccggg gcgcggcgcc
    11401 gtttccctca tcgccgggcg gcgggccgcc gtctgtttcg tccgactcat caagcatggc
    11461 ggtcattatg ggccgcaggc gcttacagca aacgcgttcg tcccgcgccc tgcaagccca
    11521 ttgaaagcgc gccatcatcg gtgggtttga cactcacccc aggagccccc tttgagcgca
    11581 cttcgcattg ccgtcgtcgt cggcagcatc cgcaaggatt cgtacaaccg ccgctttgcc
    11641 gaggcgctgg cgcggctggc gccggccggc gtcgagttca actttctgcg catcgacgac
    11701 ctgccgcact acaaccagga cgacgacaac gacaacgcgc ccgaggtcaa gcgcttcaag
    11761 gccgaggtgg aggccgccga cggcgtgctg ttcgtcacgc ccgaatacaa ccgttcgttc
    11821 tccggcgtgt tgaagaacgc catcgaccag gcttcgcgcc cctacggcca gtcgaaatgg
    11881 aagggcaagc cggccgccat cgtgtcggtg tcgccgagcc ctttgtcggc cagcatggcg
    11941 gcgcagcacc tgcgcaacgt ggtggcggtg ctgggcatgc cgacgctcaa cctgcccgag
    12001 atgtacatcc agtggaagga cggcctggtc gacgccaatg gcgacattgg cccggccagc
    12061 cgcgaattcg cgcagacctt cgtcaattcc tttgtcgact gggtgcgccg cttcaagcag
    12121 taaagtcggc tcccgtcatc cccttcaagc caggaaagaa caccaccatg agccaactca
    12181 ccgttgccgt cgtcgtcggc agcatccgca aggattcgta caaccgccag tttgctgagg
    12241 cgctggtcaa gcagcttccg gccgacttca aggcgcagtt catccgcatc gacgacctgc
    12301 cgctgtacaa ccaggacgac gacgacaacc cggcgccgca ggtcacgcgc ctgcgcgagg
    12361 agatcaaggc ggccgatgcg gtgctgtttg ccaccgccga atacaaccgc tcgatccccg
    12421 gcgtgctgaa gaacgccatc gaccacgcct cgcgcccgtg gggcaagagc gtgtggggcg
    12481 gcaagccggt gggcgtggtc ggtgtgtcgg tgggcgccgc cggcacctgc atggcgcagc
    12541 agcacctgcg caacgtgatc tcttacctga acatgccgca gttcggccag cccgagatgt
    12601 tcatccagtg gaaagaaggc ttggtgaagg acggcgccat cgggcccgac agcaaggact
    12661 tcgtgcagaa gtacgtcgac accttcgcgg cctgggtgaa aaagcaggtc ggctgagggg
    12721 ttcgcaccag acgcgcccgc agcgcccgcc acttggcggg cgttttttcg tgcggcgccg
    12781 cattttagca ttaaaatggc cgttggcgct tgaccagcaa gcgcgagcag ctatcaaagt
    12841 tgtagttttt ggcgtcggtg cgtggaactt caatgcactg ttcacgcacc caagctggcc
    12901 cgtagattcc gtcgccccgt tggagaaccg tcttgctcga cacctttttc gcccgcccac
    12961 gccgcgtgtt cggcgccatt gccgtgattt gcgccgccat gctggcgttc ggcattctct
    13021 atcttcaaaa cgtcgtcggg ctggagcctt gcccgatgtg catcgtgcag cgctacgcgc
    13081 tcatcatcgt cggcgtgttc tgcgccatcg cggcgctgtc gaagtcgccc gcgtcctggc
    13141 gcttgtgggg cctgatggcg gtggcgatgt cgtcctttgg cgcctttacg gccgcgcgcc
    13201 agagctggct gcagtggaac ccgccgccca tggccacctg cgggcgcgac ttgttcggca
    13261 tgatcgaatc gtttccgctg cagcgcgcca ttccgatgat cttcaagggc tcgggcgact
    13321 gcagtgcggt ggactggacc tttctcggcg gcaccatcgc caactggtcg ttcgtgtggt
    13381 tcgtgatttt cggcgtcgtc atcctggcgg cgctgctcaa gctgccgcgc cggggcggcg
    13441 tgatgccggc ctgagcgcgc agcccgcgcg cctttgggct aagcgcgctt taagaggcgc
    13501 aggatcacga tcaggatcac tgcgcccagc gtggccacca cgatgctgcc cagcagcccg
    13561 ccgcccacgg cgccgcccag cgagccgaac aggtagccgc ccaacagcgc acccagaatg
    13621 ccgacgacca tgtcgcccac cagcccgtag ccgccgccct tgaccagcac gcccgccagc
    13681 cagccggcaa ccaggccgac gatcaggaac cagatgattt ccatggtgca ctcctaaaac
    13741 tgtggtgaag gacatcgtcg tccagatgcc cccaatttag gtggtgcggc gcccccgcgc
    13801 agtaggaaaa ccgctcacgg gcctgacggc acgcgggccg aggcgctaca gttttttaac
    13861 cagcacctgg ctcttgcggt cccagttgta cttgcgcttg cgcgcctcgg gcagccattc
    13921 cgggtcgacc tgcgcaaagc cgcgcttgat gaaccagtgc atggtgcgcg tggtcagcac
    13981 gaagatcgat tccaacccca tggcgcgcgc gcgctgctcg atgcgcttca ggatcttctc
    14041 gccatcgccc tggccctggc tttgcggcga cacggtgacg gccgccatct cgcccgtgcg
    14101 cgcttcgggg taggggtaga gcgcggcgca ggcaaaaatc acgccgtcgt gctcgacgat
    14161 ggtgtagttg cctatgtcgc gctcgatctc ggtgcggctg cgcttgacca gcgtgccatc
    14221 tttctcgaac ggctcgatca gctgcaggat gccggccacg tcgtccggcg tggcttcgcg
    14281 caggctttcc agcttttcat cgaccaccat ggtgccgatg ccgtcgtgca cgtacacctc
    14341 cagcagcagc gcgccttcca ccgcgtaggg caggatgtgg ctgcgctcca cgccatgctc
    14401 gcatgccgtc acgcaatgct tcaggtaaaa gccgacgtcc atcggttctt cgggcagcgg
    14461 caggcgctgg atcagcgcac gggcttcggc cagcgggatc tcggtgtcga tcgggttgtc
    14521 gtccgattcc ggctccagcg gatgcacgcg gataccgggt ttttcggtca gaaagatcag
    14581 cttgtcggcc tgcagcgcca tggcgaccga ggtggcgact tcttccatcg ccaggttgaa
    14641 ggcctcgccc gtgggcgaaa agccgaaggg cgaaatcagc accagcgccc ccgcatccag
    14701 gctgcgcgtg atgcccgcca cgtccacctt gcgcaccagg ccggtgtgct tgaaatcgac
    14761 accgtcgatg atgcccatcg gccgcgcggt gaggaagttg cccgaaatca cccgcacctt
    14821 ggagcccgcc atgggcgtgt tgggcagccc ctggctgaag gcggcctcga tctcgaagcg
    14881 caactggcca gcggcttcct gcgccgaatc gagcgccact tcgtcggtga tgcgcaggcc
    14941 gtgcgcgtag cgcggctcgt gccccttggc gcgcaactgc tcgttgacct gcggccgaaa
    15001 gccgtgcacc agcacgatct tcacgcccat gctctgaatc agcgccaggt cggtggcaat
    15061 ggacgccaac ttgccggccg caatcgcctc gcccaccacc cccaccacga aggtcttgcc
    15121 gcggtgcatg tggatgtagg gcgcaacggc gcgaaaccag ggcacgaaag tgaagttgaa
    15181 cacggtggac atgcggcaga tgaaaaggcg tgggcggcgg cgggcaggcg ccaagtgtag
    15241 cggccagccg ccgcgcccgc gatgatttca ctattgtaaa tatagctgct cgcgctggat
    15301 ggacgtgcgc cagcggccta aaacatgctt gaaacagcag catgggtgcg gtgcggtctg
    15361 ggcacgcttc agacgccgct gatggggccc ggccaagggt ttgtcttagg ccggcgatcc
    15421 actggttttc aaacaatcag gccatagtcg aaccaccccg aaaaccataa ggagacgccc
    15481 gcgatgaaga tcgaactgac agtgaacggc cggccggtgg cggccgatgt gcccgaaaac
    15541 accttgctgg tgcaactgct gcgcgacacc ctgcaactga ccggcaccca tgtgggttgc
    15601 gacaccgccc agtgcggcgc ctgcaccgtg atcgtcggcg accgtgccat caagtcgtgc
    15661 aatgtgctgg cggcgcagct gcagggcgcc gaggtgacca ccattgaggg cgtggcccat
    15721 gccgacggca gcatgcaccc ggtgcaggcc gcgttcaagg actgccacgg cctgcaatgc
    15781 ggcttctgct ccaccggcat ggtgatgagc tccatcgacc tgctcaagca ccaccccaag
    15841 gccagcgagg ccgagatccg cgagctgctg gagggcaaca tctgccgctg caccggctac
    15901 cagaacatcg tcaaggcggt tcaacaagcg caggcggcgc tctaaaggag acacgacatg
    15961 ggtgccaacg actttgccaa gctgccgcac atcggcgaat cgatgaagcg caaggaagac
    16021 gagcgctttc tgaccggcaa cggccagtac accgacgaca tcacgcaggc gaaacagaaa
    16081 tatgcggtgt ttgtccgcag cccgcacgcg catgccgtta tcaaaagcgt agatattgtc
    16141 gaggcgtccg agatgcccgg cgtggtgaaa atcttcaccg gcaaggacat ggaaggcaag
    16201 atgggcgggc tgccctgcgg ctggctcatc aacaaccccg acggcagccc catgaaggag
    16261 ccgccgcacc cgatcctggc gcacagcaag gtgcgctacg tgggcgacca tgtggcgatg
    16321 gtggtggccg acacgctgca acaggccaag gacgcagcgg aaaaagtgga ggtcgattac
    16381 gaggtgctcg gccccgtcat cgacatgcgc acagccaaaa acggccccgc gctgcacgac
    16441 gaagcgcccg acaaccattg ctacaagtgg actctgggcg acaaggcggg cgtggacgaa
    16501 gcctttgcca aggccgcgca cgtcaccaag ctggacatca ccaacaaccg cttgatcccc
    16561 aacgccatgg agccgcgcgc ggccattgcc agctacaacc gcgcgacgga ggagtacgag
    16621 ctgtacacca ccagccagaa cccgcacgtg gcgcgcctgc tgatgacggc cttcgtgctc
    16681 agcctgcccg agcacaaggt gcgcgtgatc gcacccgacg tgggtggcgg tttcggctcc
    16741 aagatcttcc tctacgccga ggaggtggcc tgcacctggg cggccaagca actcaacgcc
    16801 agcatcaagt ggacggccga gcgcagcgag tccttcgtgt ctgacgcgca cgggcgcgac
    16861 cacatcacgc acgccgaaat ggcgatggac aaggacggca agttcctggc catgcgcgtg
    16921 cacaccgacg cgaatctggg cgcttacctg tccacctttg ccagcagcgt gccgaccatc
    16981 ctgtacggca ccttgctggc ggggcagtac acggcgccgc agatctacgt cgaggtcgat
    17041 ggctggttca ccagcacggc gcctgtggat gcgtaccgcg gggcagggcg gcccgaggcg
    17101 acctacgtgg tcgagcgcct ggtcacgcgc tgcgcgtggg agatgggcct gagtcaagac
    17161 gaaatccgca agcgcaactt catcaccagt ttcccgtacc agacgccggt ggcgctgcaa
    17221 tacgacatcg gcgactacaa cgcgtgcatg aacgacgccg aaaagctggc cgacgtggcc
    17281 ggcttcgagg tgcgccgcac cgccagcgag gccaagggcc tgaagcgcgg catcggctac
    17341 agcagctaca tcgaagcctg cggcctggcg ccgagcaaca tcgcgggtgc tctcggtgcg
    17401 cgtgccggct tgtttgaggc cggcgaggtg cgcgtacatc ccacgggcag cgtcacggtg
    17461 ttcaccggct cgcacagcca cgggcagggg cacgaaacga cgtttgcgca actggtggcg
    17521 gcgcggctgg gcatcgatcc aagtcaagtg gacatcgtgc acggcgacac cggccgcgtg
    17581 cccttcggca tgggcaccta tggcagccgc agcttgagcg tgggcggcac ggccatcatg
    17641 aaagcgctgg acaagatcga ggccaaggcc aagaaaatcg ccgcgcacct gatggaagcc
    17701 agcgatgccg acatcgagtt ctcaggcggc gagttcaagg tcaagggcac cgacaagaaa
    17761 atccccttcg gtcaggtggc gctgacggcg tacgtgccgc acaactaccc actcgacaag
    17821 ctggagccgg gcctgaacga aacggccttt tacgacccga ccaatttcac cttccccgca
    17881 ggcacctaca tctgcgaggt ggaagtcgac cccgccaccg gcgtggtgcg ggtggaccgc
    17941 ttcacggcgg tcgacgactt cggcgtcatc atcaacccca tgatcgtgga aggccaggtg
    18001 cacggcggcc tggtgcaggg catcggccag gcgctgatgg agcacggggt gtacgacccc
    18061 gagagcggcc agctgctcac cggcagctac atggactaca ccatgccgcg cgcgggcgac
    18121 ttccctgaat tcaagctcgg ccatgtctgc acgccctgca cgcacaaccc catcggcagc
    18181 aagggctgcg gcgaagcggg tgccatcggc tcgccgccgg ccgtcatcaa cgccgtgctg
    18241 gacgcgctca aaccgctggg cgtgaaagac ctcgacatgc ccgccacgcc gcatcgtgtg
    18301 tggcaggcga ttcagaacgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 ngtggcgaac aatgccgacg tgaaggccat gatccccgcg ctcgcatcgc tggccgaggg
    18481 catcggcgac aagcaggtgc gcgccatggg caccatcggc ggctcgctgg ccaacaacga
    18541 tccggccgcc gattaccccg ccgccgtgct ggccctgggc gccacggtca tcaccacaga
    18601 gcgcgaaatc gcggcggacg actactttca aggcatgttt gcaacggtac tggaagaggg
    18661 cgagctcatc accgccgtcc gctttcccaa gccgcagcgc tccgcgtata tgaagttccg
    18721 ccagcccgcc tcactgttcg cgctgatcgg cgtattcgtc gcacagaccc ccgacggtgc
    18781 gcgcgtggcc gtcacaggcg gcggcaacgg cgtgttccgg cagacggcca tggaacaagc
    18841 gctgaccaag agcttcacgc ccgaggccat cgccgacatc aggaccgacg cgagcgagat
    18901 caacggcgac ctgcacggta gcgccgatta ccgcgcgcac ctggtcggcg tgatggcgca
    18961 acgcgcggtg gccaaagcgg tggcttgagt gcgcgtgcgg tgcgcagccc ttcacgtggc
    19021 gcttgtgggc ccgcagattc gttggatctc acggccgtcg cagacggctg cgctgcgttg
    19081 ccaagcaggc tcaaccgccc agcgccatca cggccaggct gagcaacgtc gtcagcgcgg
    19141 ccatgaccaa gccaatgcgc atcgggtggg gatggccggc gtagcgcgcg aaggcggggc
    19201 cacacaggaa caacatcacc aacgtgatgc cgcgcgacgt gtgcagcgcc atggtggcgt
    19261 cgtccatcca cacgatgggc agcaccacgg ggaaagttgc cagcaccacc atccagaaga
    19321 tggcgatggc gggataaaaa tccgtcctgc ccagggccat tccatgctgc ggcggcaggg
    19381 ccagcaagcg ctggcgcatg gcttccagct gtttggtgtc aagcagcggg cgcagttcct
    19441 cgggcagcgc ctgggccagc acgccgcggc cggcttctgg cgacgcggcg cgcacctggc
    19501 gcgccagggt gcgcgcgtgc gagcggccga tcagggtgcg caacaagtac atcaccgcgt
    19561 ccaccatgcc ccacgccacg tttcagccca atgcggccgc gcgcaaggtc aatgcttcca
    19621 acgggcccgc gctggcgatc gacagcgagc cgatgatcgt caccgccatg atgaggccga
    19681 acagcacctc cgagatccga tccaccgggt tgagcagttg ttcgcgcacg accgcgggct
    19741 ggttttgggg ggtcatcgga agggcttctg cggacacggg ggctccatga accaccatcg
    19801 cgacggtggt gtgcgccacg gggcggcgct ggagggcatg cgcccatgaa cgcattggct
    19861 tccatcgatg gtttgcagca agagctgcaa gacgccggct atttcgccga ccgacggctg
    19921 gccacggcgg cgttcttggc actcaagttg cagcgcccgt tgctgctgga aggcgagccg
    19981 ggcgtcggca agaccgagct ggccaaggca ctggcgcaga cgctggggcg cgaactgctg
    20041 cgcctgcagt gctacgacgg actggagcaa cgagaggcga tgtacgagtg gaactacgcc
    20101 gcccaactgc tgcacctgcg tgccgtggaa ggcaaggcac cagcggagga gatagagcgc
    20161 gaggtctatc agccgcacta cctgatccgc cgaccgctgt tgcaggcgct gcaagcgccg
    20221 gcgcccgggg cggtgctgct gatcgacgag gtggatcgcg ctgacgagcc gttcgaggct
    20281 ttcctgctcg aatatctggg cgaataccag gtcagcattc ctgaactggg caccgtgcgc
    20341 gccgtctgca cgccggtgac gattctcacc agcaaccgca cgcgcgagtt gaacgatgcc
    20401 gtcaagcgcc gctgcctgta ccactggctc gattaccccg agcgcgagcg cgaactcgcc
    20461 atcgtgcgcg cccgcgcccc cgatgcgccg caggcgctgg ccgagcaggt cgcccgcttc
    20521 gtgcagcgcc tgcgcagcgc gccgtatgcg ggccactttc agcgcgcgcc cggcatcgcc
    20581 gaaagcgtgg aatgggccaa ggccctggtc gcgctcgaca cgctgcacct cgaccccgag
    20641 gtggtgcaag gcacggccgg cgtgctgttc aagcagcgcg aggatgtggc ggcgctgacg
    20701 gagccgttgg tggcggaact gcttgaggcg caggcggtcg atccatgaac ttcatacgcg
    20761 aaggacgcga agatttcgcg aaggacgcga gagactcatt tcttttgggt ttctttcgcg
    20821 tccttcgcgt agctttcgcg tccttcgcgt cctcttcggg ctgtcatgca actcggtgac
    20881 gcccgcaccg gcaagctcgc cggcaacatc gccgccttca gccgcacgct gcgccgcgcc
    20941 ggtgtgccgg tggatgcctc gcgcatcgtg ctggcccagc aggcgctgca atgcgtggag
    21001 ctggaccgca agcccgacgt cgccgccgcg ctcgaagccg tgctggtcag ccgcgagggc
    21061 gaccgcgcgg tgttccgtga gctgttcgac gccttctttc gcgatcctga actcgccaac
    21121 aagctgctcg cgcagatgct gccgcgcgcc gaaggccagg ccgagcggcc gcgccagcga
    21181 cctcgtgtgc gcgaagccct gcagccgcag cgcgcggcgc agatgctcaa gcccgccgag
    21241 cccgacaagg aaatcgacct cgacgccgcc atgacggcca gcgacgccga acgcctgcgc
    21301 caggccgatt tcaaccagct cacggccagc gaataccgcc tggtcgaacg cctcgtgcgc
    21361 gacattcggc tgccggtgcc cgccgtgccc tcgcgccgca cgcgcgccgg ctggcgcggc
    21421 caccgcgttc actggggcca cacactgcaa ggcgccatgc acaccggcgg cgaggtgatg
    21481 gcgctgcacc gcctgcaacg ccgcgtgcag cccttgccgc tgctggtgct ggtcgatgtg
    21541 tcgggctcga tggaacgcta cgcacgcctg ctgctggcct ttctgcacgc cgccacgcgg
    21601 caggcgcacg gcatgcgcat ccgccgcgac gtgttcgctt tcggcacgca cctgaccgac
    21661 ctgacgcccg ccttccgcct gcaggacgcc gacgccatgc tgaccgaggc cagccgcgcc
    21721 atcgacgact tcgccggcgg cacgcgcctt ggcgattcgc tggccgcgct gcgccgccgc
    21781 cacgcgcgcc ggctggtcgg ccgccgcacg ctggtgctga tcgtcagcga cgggctggac
    21841 acgggtgagc cggaggaact ggcgcgcgaa ctcgcctgtc tcaagcgcca cacgcggcgc
    21901 ctgctgtggc tcaatccgct gctgcgtttt gacggctacg cgcccaccgc gcgcggtgcg
    21961 gccgaactgc accgcgccgc cgacggcatg ctggcggtgc acaacctgag cgccttgcag
    22021 acgctcgggc acgccattgc cgcgctcatt caacaatagc aagacaagga gattccacat
    22081 ggacatgcaa ggcagcaggc aactcgccgt gacgcagcaa caggcgtggg aagcgctgaa
    22141 cgaccccgag gtgctgaagg tctgcatccc cggctgcgac agcatcgaag ccacgggcga
    22201 gggcgcctac gcgctgacca acgccatcaa ggtcggcccc gtgtcggcca agttcaaggg
    22261 caacctgcaa ctgaccgaca tccaggcgcc cgacagctac accatcaact tcgagggcaa
    22321 tggcggcgcc gctggcttcg gcaagggcag cgccaaggtc aatctggtgc ccaacgacgc
    22381 gggttgcgag ctgggctaca ccgtcaacgc ctcggtcggc ggcaagatcg cccaggtcgg
    22441 ccagcgcctg atcgatggcg tggccaagtc gatggccgag agcttcttca agcgcttcga
    22501 cgaagaaatg aaccgccgcc atggcccgcc gcccgatgag gccactgccg aagagcccgg
    22561 caagctcaag aggatgtggg gcaaactgac aggcagcagc aaggctgaag acgccggctg
    22621 aacgtgaaag gagcccctca tggaaaacct cgacaccgcc gtgctgcgcg cgctgcgcga
    22681 ctggcgcgcg gccggtcagc aggcgctgtt ggtcaccgtg gtgcgcacct ggggttcgtc
    22741 gccgcgcccg gtgggctcga tcatggcgct gaaaaaaaat ggctcggtgg ccggctcggt
    22801 gtcgggcggc tgcatcgaag acgacctgat tgcccgctac gcgcgccgcg gcgcggccga
    22861 tgcgctgccc cacagtggaa aaccgcagtt cgccaaatac ggcatcaccg ccgacgaggc
    22921 gcaccgcttc ggcctgccct gcggcggcac gctggagctg ctgtttgagt tcgaccccga
    22981 cgccgccagt ctgacggaac tggtgcgcgc tctcgacgcc ggccgcctga tgcgccgcac
    23041 ggtgcatctg gcggatggcc gcgtcacact cgcacccgcc gacgcgccga ccgaactgaa
    23101 cgtggacgac gcgcagctga ccaatacctt cggccccgaa taccgcatgc tcatcatcgg
    23161 tgctgggcag atgagcgaat acctcgccac catggcgcag ttctgcggct tcgccgtgac
    23221 ggtgtgcgac ccgcgcgagg aataccgcgg cggctggtcg gtgcccggcg tgcacctggc
    23281 caccgacatg cccgacgacg tggtcacggc attcaagccc gacaagcgca gttgcgtcat
    23341 cgccctcacg cacgacccca agctggacga tctggcgttg ctggaggcgc tgggcagcga
    23401 agccttctac atcggcgccc tgggcagccg ccgcaacaac acggcacggc gccagcggct
    23461 gatcgagcac ttcgatcaga ctgaagccag cctcgccaaa ctgcgcggcc cggtcggcat
    23521 cttcatcggc agcaagaccc cgcccgagat cgccgccagc gtgatggccg aggtgctggc
    23581 ggtgaagaac ggcgtcaccc tgccgcgtga cctggaggtg gcgcacgtga aagaccagca
    23641 ggcagcgcag ccaggccgca cggcttcgtc gtgcgcgatg ggttgatctg cgtcggcgca
    23701 tggccctgga tgctattcaa gacatagcgg gtatcgtcct ggcggcgggc gctggccgcc
    23761 ggatgggcta caggcccaag gcactgctgc agcgcgatgg ccagccgctg gtggcgcgcc
    23821 agatcgggct gctggcccag tgcagtgtgc gccgtatcat ggtcgtgctg ggccaccatg
    23881 ccgcacgcat cgcgcccgtg ttggcgacca cggcgacggc gcttcccggc gttgacctga
    23941 tctgggtggt caaccccaca cccgacgaag gcacgggcgc gtcgttgcgt tgcgcgctgg
    24001 cggccttgcc ggccgacttg acgggtgtga tggtgctgct ggccgatcag cccttgctgc
    24061 aggttgatga cgtggcctgc gtgctggctg cgtggcgcgc gagaccagcg gacatcgact
    24121 tgatcgtgcc cacgcatgaa ggccaacccg gccacccgct tatttttggc ccacaggtgc
    24181 ggcaaggggt ggcgaacggg cagggcgctg cgggcgtgcg cgactggcgg cgcgcgcacc
    24241 cgcgccaggt gttggcgctg ccggtcgatc acgcgcgctg cacgcaggac atcgacaccg
    24301 aggatgatct ggcgcggctg acggctgagc agggggtgcg cctgagttgg cctgacgtga
    24361 cttgattttc aaacagaaca gggcgccagc gcacagcagt caagcgcgag cagctattca
    24421 agcaatagca aacagctcaa tttcccgcaa acgcctccca ccgcgcatgc catggctcgg
    24481 gcccgttcgc gtcgccttgt gcatcgtcca cgcgcacggc aagtccggtc acgtgcccga
    24541 acgttgccgc cggctccgcg ccatcggcca gcagctgccg caccagttgg cgaaacgccg
    24601 tgccgtgctc ttgcagcaac gtggcggcgc gcgccagctc ggcggccagc agnnnnnnnn
    24661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgtgagc tgcgtcttgc cagtgccggt
    24781 caggccccac tggcagaaga tgacggggcg cttgatgatc tcgggcagca cgtaccaggc
    24841 gcgcaccgct tcaatgacgc ggtcgatcac ctcgtcgata ccgagcagct cggtcttcag
    24901 ctcggcggcg atggccgcca gttgggcctt gcgctcggcc aggcgttggt tcaagggctc
    24961 ccgttgcagg aagcgggtgt cgtgtaagtc catgtcaatc gttccatgca ttgcgaacgg
    25021 ctttttgcaa agccacgcgg tcggcgcggc gctgcgcgcc ctggctgcgg acgtgcttac
    25081 cggcgccact gctgacgctg cgtgtggcca tggccagcgc gatggggttg cgcggcggcg
    25141 gcaattgcag cgacaacggc gcgcgcatac gccgcagttg tttgaggttg gcctgcgcgc
    25201 cgccagcggc agcgcgcgag aaggttttgg cactcatgtg cactccgaca agcaaaaaag
    25261 ccccggcaac aagcggtgtg ccgtgggctt ttaggggcct gcggctgaaa tggcgaatgc
    25321 attccagccg gggcggatgg agtgcgtcag atgggtgcga gaagaatcaa ggtcatcttt
    25381 caaaacctcc tatgacttgc gcaataagga cagagcacag cgcttcggtg gcgcgatgcg
    25441 ctttgtctgt gaggattgcg caattcctac gttggattgg atccggtgcc ggcaaggttg
    25501 ctggttgccg gcggcgtgga acgcaggaga aggcgcggat tgtgcggcaa cccgccggtt
    25561 cttgccaagc ggcttgtcgc gtgcatgcga cgcgcatggg caacgcgagc gagtcaagcg
    25621 cgcgactgcg caccgctgaa cgactggcac gatgtgccca caatcgaccg ttgctcatca
    25681 ctcaaccgga ggacttcgcc atgcgaaccc gcatcaccga cctgctcggc attcaatacc
    25741 ccatcgtaca aggcggcatg cacttcgtcg gcttggccga actggcgtca gccgtgtcca
    25801 acgccggcgg tctgggcata ctgaccgcgc tcacgcaacc gtcgcccgcg gcgttggctg
    25861 aggagatcgc acgctgccgc gcgatgaccg acaagccctt cggcgtcaac ctgacgtttc
    25921 tgcccgcgcg cacgccgcca gactaccccg gctacgtgcg caccatcgtg gatggcggca
    25981 tcaaggtggt cgagacggcg ggcaacaacc cgcagaagtg gatgccgatg ctgaaggagg
    26041 cgggcatcaa ggtcatccac aaatgcacct cggtgcgcca cgcgctgaag gccgaggcga
    26101 ttggctgcga tgccgtgagc gtggacggct tcgagtgcgg cggccacccc ggcgaggacg
    26161 acgtgcccaa cttcatcctg ctgccgcgcg cggcgcaaga gttgaagatc cccttcatcg
    26221 cctctggcgg catggccgat gggcgcagtc tggtggcagc gatggccatg ggcgccgagg
    26281 gcatgaacat gggcacgcgc ttcattgcga cgaaagaggc accggtgcac gagaaggtca
    26341 agcaagccat tgttgccgcg agcgaactcg acacgcgtct cgtgatgcgc ccgctgcgca
    26401 acaccgagcg cgtgctgagc aacgccggcg tcgagcgcct gctggccaag gagcgcgcgc
    26461 tgggcgacaa gatcacgttc gaggacatca ttgccgaggt cggcggcgtg tacccgcgtg
    26521 tgatgaagga tggcgacctg gacgccggcg cctggtcatg cggcatggtg gcggggctga
    26581 tccacgacat ccccaccgtg aaggagttga tcgaccgcat cgtggccgag gcgcagcaga
    26641 tcgtgcgcga gcggctggcg gggatggtgt gacgcggttg cgtcaccatt ttgattgaat
    26701 cggctgctcg cgcttgccag acaagcgcga gcagctatca attaagtagt tccaagcgcg
    26761 ggctatcgac tcactttttc ttcggcacga tgtccaccac ccacaactca gccgcttcgg
    26821 tggtgctggg gttcacgaac cagtggctgg tgtcaaaagt ctccagccag aaatcaccgg
    26881 gcttcagcac gcgcttggcg tcgccgcggc cttcgaccac cgtgccttta accacgcgca
    26941 cgatgcctgg gcggccggcg tgtgggtggt tgcccacgcc gccgccgggc gcgagggtca
    27001 gcgcgcgggc gcgcaggtcg tggtcggcgg gcgtgccggc catcttgtgc gcggccagat
    27061 cgaccaggcc cacgggtgtg tcgccgctca ggccgctgtt ggccgttggg gcgctgagct
    27121 tggcggtatc gggcgccgag gcgcaggcgg tgagggcggc caccacggcc agtgtgcaga
    27181 cggtgcgaat cattgagttt ctactccggt tgaatttgca ggggttgggg cgcacatgct
    27241 acgagcgccg cgcgggtttt cgccgtggcc gcgcactggg ataatcccgc accccatgac
    27301 tgaacagcgc cccatttctc aacccgaatc gccccgcagc gcgcgcccgg cgcgccagag
    27361 ccgtcctgaa gttgtagcag ccaaccagcc caaggtgctc gactttccgg ccaacctgcc
    27421 ggtcagcgcg cgcaaggacg agatcatggc ggcgctgcac gcgcatcagg t
//
LOCUS       DAOMVO010000003        26573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_272_length_26573_cov_14.284184, whole genome shotgun sequence.
ACCESSION   DAOMVO010000003 DAOMVO010000000
VERSION     DAOMVO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 26573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26573
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_26573_cov_14.284184"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKV30_00365"
     CDS             complement(<1..627)
                     /locus_tag="PKV30_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL28705.1"
                     /translation="MTPRFSLSWLVLTAVAGAALLSYALPAQSFIAYAAQGVEVAQIV
                     PLTGPLANVGKEVNAITKATLEDFNKGSKTTQIVLKPYDDGNVADRSTRLATKAVASA
                     QALISCFGSVGCLPQQKVSATAGVPLIGPIAGAAPLRGKAATTTYAVRASASSEVACL
                     LNFASTTGLSNVSVLMQDDGFGKSYAAELDRAAANFPGLKITRSAFNPA"
     gene            complement(624..1598)
                     /gene="puuE"
                     /locus_tag="PKV30_00370"
     CDS             complement(624..1598)
                     /gene="puuE"
                     /locus_tag="PKV30_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HRL28706.1"
                     /translation="MKPSIYDSTAAYPRDLVGYGADVPHARWPGGARIAVQFVLNYEE
                     GGENSVLHGDAASEQFLSEMFNPAAYPDRHLSMEGIYEYGSRAGVWRILREFDARGWP
                     LTVFGVATALARYPEMVKVFQQRGDEIACHGLKWIHYQNVDEATERAHMAEALDIIEK
                     LSGERPLGWYTGRDSPRTRRLVADDGRLLYDSDYYGDDLPFWMKVQRTDGAVVPHLVV
                     PYTLDCNDMRFALPQGYSHADPFFEYMKDTFDALYAEGDPHGLDRPKMMSIGMHCRLL
                     GRPGRITALQRFMDHVSRHKNVWVCRRIDIARHWEETYPFQTSPEVST"
     gene            complement(1628..2299)
                     /locus_tag="PKV30_00375"
     CDS             complement(1628..2299)
                     /locus_tag="PKV30_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL28707.1"
                     /translation="MEPSATDTIVAALTRAIVEHRLQPGAKLTEQTLAGHFDVSRTLV
                     RQALFQLSQRRLVTLEPARGAFVASPSAEEARQVFAVRRMLEVELTRAFVAAATPAHI
                     DALRDHVAREQAAVQRQDVEGRTELLGDFHVLMAELLGNHVLADVLQDLLARCAIATL
                     MYQSSHAAHDSSAEHAALVECFAAGNVTRAVKLMREHLDHVEAGLKLDQPVPSHDVKS
                     ALAPV"
     gene            2377..2730
                     /gene="uraH"
                     /locus_tag="PKV30_00380"
     CDS             2377..2730
                     /gene="uraH"
                     /locus_tag="PKV30_00380"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRL28708.1"
                     /translation="MGLSTHVLDTMHGTPAAGMAVALYETHGEVATLVKRFTLNADGR
                     NPDGPLYDNASLKAGTYRLAFDVAGYFRARGVQLPEPPFLNVVNLDFGIAHVDQHYHV
                     PLLCSPWSYSTYRGS"
     gene            complement(2774..3355)
                     /locus_tag="PKV30_00385"
     CDS             complement(2774..3355)
                     /locus_tag="PKV30_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28709.1"
                     /translation="MRTPHRTIPRPWQIRSLMLAMTIACAALAWAPTPAEAQSKAAPT
                     KTVRPSTRKPAPPIKPRKRVRPAAGQSSARPVPVPEASDAATARAYRKDAARHLYSLN
                     LGSIYKGRMPPLLYAVGVLQVHIDGQGRVGTISWMRAPSHAPEVMRAIERMVRAADPF
                     PPPALVGGLVYTDTWLWHKSGQWQLDTLTEGQD"
     gene            3423..4262
                     /gene="xdhC"
                     /locus_tag="PKV30_00390"
     CDS             3423..4262
                     /gene="xdhC"
                     /locus_tag="PKV30_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HRL28710.1"
                     /translation="MALSQRVPQVQDLLLHLRQAPALLVSVTRTAGSVPREAGAWLAV
                     CGDAVIGTIGGGQLEHQAIAHARELLAGLVTAAHETRRYPLGPSLGQCCGGVVELQYE
                     HVSAIDMEALHARLSSATGHLALFGGGHVGRAIVRLLADLPFSVRWIDSRDEVFPPDV
                     PANVQCEHSDPVQVAVADLPANSAILIMSFSHAEDLDIVAAALTRQRARGDVRYIGLI
                     GSKTKWATFRHRLSVRGFSDAELQQVICPIGLPAIRGKRPAVIAVAVVAQLLALGLAE
                     PAA"
     gene            complement(4326..4484)
                     /locus_tag="PKV30_00395"
     CDS             complement(4326..4484)
                     /locus_tag="PKV30_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28711.1"
                     /translation="MGNKIVTEASRKATAPLPGATVAKQGVGQRKSQERGSHTRSKKN
                     PGKRPHQG"
     gene            4776..5993
                     /locus_tag="PKV30_00400"
     CDS             4776..5993
                     /locus_tag="PKV30_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRL28712.1"
                     /translation="MEAYLLDWANLLLRWLHVIVAIAWIGSSFYFVFLDNNLLAPTAD
                     DLKKKGVNGAMWAVHGGGFYNPQKYMVAPQGGIIDSKLHWFYWESYSTWLSGFALFCV
                     LYLWNASAFLVDKQLLDWSPVAAGVAAVGFMVAFWFIYDTICRVFGFRPNGERIVAAL
                     LIVVVALATWLACHLFSGRAAFLIIGAMLATSMSANVFFWIIPGQRKVVAAMTSGQPY
                     DADALAIHGKRGKQRSVHNTYFTLPVLIAMLSNHYSFLYSHPQRWLVLVLMMLAGALI
                     RQFFVQRHGWHHGRASNPWPFAAAGVVILLGVIAWLRPAPAPAAAAAPAVVDYAQLNP
                     ILEQRCYSCHGEAVQMKNLRLDSADSVKSHAQQIYQQVVVTKIMPLSNATQMTDAERQ
                     LVARWFEGQGSRP"
     gene            complement(6040..6630)
                     /locus_tag="PKV30_00405"
     CDS             complement(6040..6630)
                     /locus_tag="PKV30_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28713.1"
                     /translation="MARPTKPDALELAQGHTRLEQAVLRSRQLLRKRAMVAGVAGAVP
                     LPGLDWAVDAAMLTQLLPKINEEFGLAPHQIAKLDAREKERMQKAIAMIGSVIAGRLV
                     TKALVVRFARLVGVRLTAAQAARYVPLAGQVVSGVLGYTALRYLGEQHIRDCVRVALA
                     APLALPAPEAASAATDRRRQRQGQTISSSLGPIILH"
     gene            complement(6676..6924)
                     /locus_tag="PKV30_00410"
     CDS             complement(6676..6924)
                     /locus_tag="PKV30_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRL28714.1"
                     /translation="MGLIVTIIVGFIVGLIARAIMPGNQSMGLIMTTILGIVGALLAS
                     YGGAVLGLYPAGSPVGWIASIIGAIVVLFLYGLVTKRR"
     gene            complement(7070..7834)
                     /locus_tag="PKV30_00415"
     CDS             complement(7070..7834)
                     /locus_tag="PKV30_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRL28715.1"
                     /translation="MKGRLFLVPTPLDHGCAEQTPLDDVLPLGTIKKAAAIACWIGEN
                     AKSTRAFLKRVDALVPLAQPIQTQQITELPRDVHKKGDHQTSATWDARPLLAAALAGQ
                     DVGLVSEAGMPAVADPGSSVVRAAHQLGIEVLPLTGPVSLLLALAASGLNGQNFAFIG
                     YLPQDASARTTQIQQISRAVQQSGQTRIVIETPYRNQVLLEALLSGLDGQLRLCVASG
                     LTLPDADGARSASVAEWRQARWPVTPKAPAVFLIGR"
     gene            complement(7831..8439)
                     /locus_tag="PKV30_00420"
     CDS             complement(7831..8439)
                     /locus_tag="PKV30_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRL28716.1"
                     /translation="MTTDSTGSASARALVLGSTSRYRRELLTRLGLPFTTEAPGVDET
                     PQKGESPRDLAVRLALAKARDVARRHPQAIVIGSDQVADLAGQPLGKPGNHERAVAQL
                     RAMRGQTVIFQTAVSVVCQATGFKQSDLAAVEVRFRALEDDEIERYLRAEQPYDCAGS
                     AKSEGLGIALLDAILSDDPTALIGLPLIRTARLLRAAGLRLP"
     gene            8466..9017
                     /locus_tag="PKV30_00425"
     CDS             8466..9017
                     /locus_tag="PKV30_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRL28717.1"
                     /translation="MSKTFKAQRLDVAAFADAKAVLSGTESLQKYERLAQELQAPAPD
                     LTLDWQATGGARKAANGSVYPSVHLQVQATLPLTCQRCMGEVTEAVEVDRHFLFVPDE
                     ATAAALDDESDDDVLAIAPDFNLQALIEDEVLMALPLVPRHEVCPDPVRLGAQTDDFD
                     SAGEAKRQPFAALAALKSEKKSG"
     gene            9182..9364
                     /gene="rpmF"
                     /locus_tag="PKV30_00430"
     CDS             9182..9364
                     /gene="rpmF"
                     /locus_tag="PKV30_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRL28718.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNGLATPGIAVEPTTGETHLRHHISP
                     NGFYRGRQVIKSKTEA"
     gene            9536..10588
                     /gene="plsX"
                     /locus_tag="PKV30_00435"
     CDS             9536..10588
                     /gene="plsX"
                     /locus_tag="PKV30_00435"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRL28719.1"
                     /translation="MIVLAVDCMGGDHGPKVTLPACRQFLDARPDAQLMLVGQAEALA
                     GFAHERARVVPATEVVTMDDAVEIALRKKKDSSMRVAIQQVKDGAAQAAVSAGNTGAL
                     MAIARYLLKTLDGIERPAIATQIPNAQGGATTVLDLGANVDCTADHLLQFAVMGSALV
                     SVLKDDPQPTVGLLNIGEEAIKGSDEIKRAGELLRAAAQAGDLNFVGNVEGNDIFKGT
                     SDLVVCDGFVGNVLLKTSEGLATMIGQFLREEFSRNILTKLAAIVSYPVLKALKKRMD
                     HRRYNGAALLGLRGLVFKSHGSADVMAFEQALARAYDAATHQLLDRLQSRIAHAAPLL
                     ASPAAQRLDAAATPAG"
     gene            10637..11617
                     /locus_tag="PKV30_00440"
     CDS             10637..11617
                     /locus_tag="PKV30_00440"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRL28720.1"
                     /translation="MTRRYSRITGTGSYLPPRRVTNDDLVTELGARGIETSDQWIVER
                     TGIRARHFAEPDVPASDLAVPAAQAALQAAGLQAADIDLIIVATSTPDMVFPSTAAIV
                     QHKLGIAGCPVFDVQAVCSGFVYALTVADALITNGTATKALVIGAEVFSRILDFNDRT
                     TCVLFGDGAGAVVVEASDTPGILATDLHADGKHVGILCVPGTVAGGQVLGDPLLKMDG
                     QAVFKLAVGVLDKAARAVLAKAGKSEADIDWLIPHQANIRIMQSTAKKLHLPQEKVVV
                     TVHDHGNTSAASIPLALDHGVRAGQVKPGQTVLLEGVGAGFTWGAVLLIL"
     gene            11679..12629
                     /gene="fabD"
                     /locus_tag="PKV30_00445"
     CDS             11679..12629
                     /gene="fabD"
                     /locus_tag="PKV30_00445"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRL28721.1"
                     /translation="MKPIAFVFPGQGSQAVGMLDAWGDHPAVRETLAEASDALGEDIG
                     ALIANGPKEDLALTTNTQPVMLVAGVACWRAWRAEGGALPAAVAGHSLGEYSALVAAE
                     ALPLAEAVPLVRLRAQAMQEAVPVGTGAMAAILGLEASKVIAGCAEVTGAMGQNGTEV
                     VEAVNFNDPVQTVIAGTKAAVEKACEVLKGMGAKRALPLPVSAPFHSSLMKPAAERLK
                     GALEAVDFADPKIPVVNNIDVAIESDHDRIRDALYRQAFGPVRWVECIHKIKAMGLHT
                     VVECGPGKVLAGMVKRIDADLTGLAVYDPATLAEAKAQLG"
     gene            12694..13443
                     /gene="fabG"
                     /locus_tag="PKV30_00450"
     CDS             12694..13443
                     /gene="fabG"
                     /locus_tag="PKV30_00450"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRL28722.1"
                     /translation="MSEVHGANRVALVTGASRGIGAAIAQELAARGYRVIGTATTDAG
                     AEKISAALAAHPGCRGAALDVNDAAAAEALIEAIVKEHGALHVLVNNAGITRDQLAMR
                     MKDDDWDAVLDTNLKAVFRMSRAVMRPMMKQRQGRIINITSVVGASGNPGQANYAAAK
                     AGVAGMTRALARELGSRGITVNCVAPGFIATDMTAALGDEQQKALTGQIPLGRLGAPA
                     DIAAAVAYLASPEAGYVTGQELHVNGGMFMA"
     gene            13536..13772
                     /gene="acpP"
                     /locus_tag="PKV30_00455"
     CDS             13536..13772
                     /gene="acpP"
                     /locus_tag="PKV30_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRL28723.1"
                     /translation="MSDIEARVKKIIAEQLGVEESQVTNEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEDAEKITTVQAAIDYANSKKA"
     gene            13889..15133
                     /gene="fabF"
                     /locus_tag="PKV30_00460"
     CDS             13889..15133
                     /gene="fabF"
                     /locus_tag="PKV30_00460"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRL28724.1"
                     /translation="MNRRRVVVTGLGCVSPVGNTVAESWASLLAGRSGIDLITQFDAS
                     ALACRFAGEVKGFNVGDYIAEKEARHMDRFIHLGLAAAAQAVADSGLPTGDALSSDQA
                     ERFGCNIGSGIGGLPMIEQTHAEYASRGPRRISPFFVPASIINMISGHASIRFGFKGP
                     NIAIVTACTSGLHAIGLSARLIQHGDADVMVAGGAESTVSPLGVGGFAAARALSTRND
                     DPKTASRPWDKDRDGFVLGEGAGVVVLEEYEHAKARGAKIYAELIGFGMSGDAFHMTA
                     PDVDGPRRSMANALRDAGVNANAVQYLNAHGTSTPLGDLNETNAIKAALGHAARQVVV
                     SSTKSMTGHLLGGAGGIESVFTVMALHEQKVPPTINIFNQDPECDLDYCANTARDMKM
                     DVALKNNFGFGGTNGSLVFKRV"
     gene            15174..15668
                     /locus_tag="PKV30_00465"
     CDS             15174..15668
                     /locus_tag="PKV30_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28725.1"
                     /translation="MDRAPSVVYPVGRTRALAWVLAVLAGSGAVLLFSVLFWPMTPVQ
                     RAPEAIVLIVSWLGAVAAMWHFWRRQRPHQLRWDGECWFVVAPTDAVEQGGEGARVEV
                     RWDAQRWMLLWFHPGAAGERGRWLWAEAASEPARWHLLRCALYLPLTSASVGAPPSSE
                     AGRA"
     gene            15705..16337
                     /gene="rpoE"
                     /locus_tag="PKV30_00470"
     CDS             15705..16337
                     /gene="rpoE"
                     /locus_tag="PKV30_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HRL28726.1"
                     /translation="MTDRPAAPPAPTDSDALLVERAAAGDHRAYELLVIKYQRRIERL
                     IGRMVRDVDLVPDIAQETFIRAWRALHQFRGDAQFYTWLYRIAVNTAKKSLMELKRDP
                     VMTESALKSVDDDDETSRRENELTSESTPESELAAREIAAAVNAAMEALPEDLRQAVT
                     LREIEGLSYEEIAEAMNCPIGTVRSRIFRAREAISARVKPLLSHQTGKRW"
     gene            16373..17014
                     /locus_tag="PKV30_00475"
     CDS             16373..17014
                     /locus_tag="PKV30_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HRL28727.1"
                     /translation="MKTQLDIPCDRETVSALMDGQLSGAQAAMAVQSMASTEARECWH
                     VYHLVGDVLRSTDLAACGHDAAFVARLAARLQHETPAFEALPALARPAMLDVARPAAN
                     DGVFRWKLVAGLASFAAVAAIGWGALGSIGPQPGAGAQLARSAAPAVGTTQVMALAAP
                     VPAASQAADMAPVMLRDARLDELLAAHRQTVGASALGNAAGFLRNATFEGPGR"
     gene            17011..18108
                     /locus_tag="PKV30_00480"
     CDS             17011..18108
                     /locus_tag="PKV30_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HRL28728.1"
                     /translation="MIAGLSSGDRAAGARRVPARGWRLRLRQAAVICCAGSLVTLAGA
                     QTGGSFGGGASERPIGPPQRSMAEWLVRLQQASRVPSYVGTFVVSSASGAMSSARIWH
                     VCEGDVQIERVEALTGAPRSTFRRNDSVMTFLPDARVVKLDQSESGGIFPNLLNINQA
                     FPTSDFYASRELGQGRVAGFDADIVMLSPRDEWRYGYRIWSEKRTGLVVKTQTVDAGG
                     RVLEQAAFSELQLGVPVQADKLRQMMGSTEGYRVVKAERTRTTPEAEGWQLKNPVPGF
                     RSENCYRRSAAKESPLVQWIFSDGLVTVSLFMEPFDRQRHQGEGVASMGATHTLTRRL
                     VDRAGDWWVTAVGEVPLPTLQAFVQGLQRRP"
     gene            18143..19660
                     /locus_tag="PKV30_00485"
     CDS             18143..19660
                     /locus_tag="PKV30_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRL28729.1"
                     /translation="MRVESSMSTMRKQWGLIALATPLLLAGSLAVPTTAAAQGPAAVA
                     PSVRGLPDFTDLVDQVGPAVVNIRTVERVRAGAAGRGGQMDQEMQEFFRRFFGIPMPN
                     TPRPQGPRADPQEQPEREGPRGVGSGFIISADGFVMTNAHVVDGADEVLVTLPDKREF
                     KAKLIGADKRTDVAVVKIDSTGLPFVRIGDINRLRVGEWVMAIGSPFGLENTVTAGIV
                     SAKQRDTGDYLPFIQTDVAVNPGNSGGPLINMRGEVVGVNSQIYSRSGGFMGISFAIP
                     IDEAMRVSEQLRSSGRVTRGRIGVQIAEVTREVAESIGLGKPQGALVRSVETGSPADK
                     AGIEAGDIITRFEGRAIEKASDLPRLVGNAKPGSRGAITVFRRGQSRDLNVTVAELEP
                     EQTANARRPAARSSEPAKPAGAGQALGLTVTDLTDAQKKELKAKRGVRVEAAADAGAR
                     AGLREGDVILSVANVDIQNVREFEAAVAKADRSKPINMLVQRGELVQFVLVRPPR"
     gene            20138..21946
                     /gene="lepA"
                     /locus_tag="PKV30_00490"
     CDS             20138..21946
                     /gene="lepA"
                     /locus_tag="PKV30_00490"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRL28730.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLISRCGGLTDREMSEQVLDSM
                     DIEKERGITIKAQTAALQYTARNGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVLPVLNKMDLPQADPDNAKAEIEDVIGIDASDVIA
                     ISAKTGMGIDDVLEQIVAKVPAPRGQPNAPLRAMIIDSWFDSYVGVVMLVRVVDGRLQ
                     KGERFKMMATGAAYEANNLGVFTPATLPRDELKAGEVGYIIAGIKELKAAKVGDTITL
                     EKKLPNNLGPAEKALPGFKEIQPQVFAGLYPTEANQYDALRDSLEKLQLNDASLHFEP
                     EVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPSVVYQVEKADGEVLMVEN
                     PSKMPDQGKINEIREPIVTVHLYMPQEYVGPVMTLANQKRGVQLNMAYHGRQVMLTYE
                     LPLGEIVLDFFDKLKSVSRGYASMDYEFKEYRPSDVVKVDILLNGEKVDALSIIVHRS
                     QSQYRGRAVAAKMREIISRQQFDVAIQAAIGANVIARENIKALRKNVLAKCYGGDITR
                     KRKLLEKQKAGKKRMKQIGSVEVPQEAFLAILQVED"
     gene            21946..22914
                     /gene="lepB"
                     /locus_tag="PKV30_00495"
     CDS             21946..22914
                     /gene="lepB"
                     /locus_tag="PKV30_00495"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRL28731.1"
                     /translation="MPILTAIILAAFVGYGASWYFGAIEGNFALLLFMATVVTGVYWL
                     AERFIFLPRRQAAAAALQQQADQRRSELAAQGITQVDEGDMARARERVLAQPWWLDWT
                     AGLFPVILIVFALRSFVFEPFKIPSGSMIPTLLVGDLILVNKFTYGLKLPVVNSRLTE
                     GNAVQRGDVVVFRYPPQPSVDYIKRVVGLPGDEVAYLNKRLTINGQPVATTAVSDFFD
                     ESTMEYFKQFDEQLGVKNHRIIVDERRPGFVAGAFDFQYKDNCRYNVEGVTCKVPEGQ
                     YFVMGDNRDNSLDSRFWGFVPDRNIVGKAFFVWMNFGDLKRIGSFN"
     gene            22934..23302
                     /locus_tag="PKV30_00500"
     CDS             22934..23302
                     /locus_tag="PKV30_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HRL28732.1"
                     /translation="MTYQRFHSQRGISFFGLLIVGVVLALLAIVGARVLPTATEFMAI
                     QKAAKKAAAEGGTVPQVRAAFDRTAAVDYISSISGKDLEVTKDGDKVVVQFAYDKEIP
                     LAGPAYLLLKYRGSSAGGYN"
     gene            23328..24017
                     /gene="rnc"
                     /locus_tag="PKV30_00505"
     CDS             23328..24017
                     /gene="rnc"
                     /locus_tag="PKV30_00505"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRL28733.1"
                     /translation="MSAPVTLQALQQRLQHQFANQSLLEQALTHRSFSADHNERLEFL
                     GDSVLNLAVAHMLYQALGSGAEGDLSRLRAQLVRQDSLHRLALDLGFAPLLRLGAGEV
                     SSGGQRRPSILADALEAIIGAVYLDGGPLAADALVRRLFERVEISPATSAAAKDAKTA
                     LQEWLQGRKMRLPQYEVARVLGAAHRQTFEVVCQVAALGLEASGQGVSRRAAEQAAAA
                     AMLQQVQNTQT"
     gene            24014..25003
                     /gene="era"
                     /locus_tag="PKV30_00510"
     CDS             24014..25003
                     /gene="era"
                     /locus_tag="PKV30_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRL28734.1"
                     /translation="MNTPEKIAADADPTSTGGPLGTNSPATGQRCGVIAIVGKPNVGK
                     STLLNALVGQKISITSRKAQTTRHRITGIRTLPDGDGGGTQFIFVDTPGFQTRHSTAL
                     NKSLNRTVLGAIDGVDLVLFVVEAGSFTLGDAKVLALLQPGTPTLLVANKLDTVHRRA
                     EIAPWLRDMQERHPFAEFVPMSAKNPKDVQRLLQICERYLPEQPWWYGQDELTDKSEK
                     FLASEVVREKLFRLTGDELPYTSTVVIEKFDEEKSKQHGRMVRIAATIVVERDGHKAM
                     VIGEKGERLKRIGTEARQELEKLMDCKVFLELWVKVRSGWADDEARVRSFGYE"
     gene            25020..25451
                     /locus_tag="PKV30_00515"
     CDS             25020..25451
                     /locus_tag="PKV30_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HRL28735.1"
                     /translation="MRLWTLHPRHLDPQGLVALWREALLAQAVLFGRTRGYTRHPQLQ
                     RFAEHAAPLDAVGAYLTAVAEEASARGYRFDASKIDRPSAQAAPITATTGQIEHEWRH
                     LGAKLAARNTDWHARWGAAGQPATHPLFTVVPGPVQDWERP"
     gene            25448..26209
                     /gene="recO"
                     /locus_tag="PKV30_00520"
     CDS             25448..26209
                     /gene="recO"
                     /locus_tag="PKV30_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRL28736.1"
                     /translation="MKRVTHEPGYVLHHYDWSESSVIVEAFTRAHGRVALVAKGAKRP
                     SSNFRPVLLPLQQLALSWGGDAEIRTLKSAEWGGGQVMPTGDALLSGYYANELLMRLL
                     AREDAHPRLFDAYAALVHVLASHEAELLVAAALRAFELLLLRETGHLPALDAQTLTFA
                     PLSDDERYRLVAEGGLRAAHADDSAWLHGAHWRALTAALADEAPLSATLNTVAELPGD
                     ARAQLRQQLRELLHYHCGVGTLRTRQLLMDIQALS"
     gene            26206..>26573
                     /locus_tag="PKV30_00525"
     CDS             26206..>26573
                     /locus_tag="PKV30_00525"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRL28737.1"
                     /translation="MNTTTQLSVNVNKVALVRNTRHLGIPSVTRAAELCLQAGAHGIT
                     VHPRPDERHIRAADVRELAALMKQWPGREYNIEGNPTQNLMAFIREVRPQQATFVPDS
                     EDQFTSDHGWDLADAAVRERL"
BASE COUNT         4499 a         8574 c         8927 g         4573 t
ORIGIN      
        1 tgcgggattg aacgcactgc gcgtgatctt gagccctggg aagtttgcgg cggccctgtc
       61 cagttcggcg gcgtaggatt tgccgaagcc gtcgtcctgc atcagcacgc tcacgttcga
      121 caggcccgtg gtgctggcga agttgagcag gcacgccacc tcgctcgacg ccgaggcgcg
      181 caccgcgtaa gtggtggtgg ccgccttgcc gcgcaagggc gcggcgccag cgatcgggcc
      241 gatcaggggc acacccgccg tggcactgac tttttgctgc ggcaggcagc cgaccgagcc
      301 gaagcacgaa atcagcgcct gggccgaggc cacggctttc gtggccagtc gcgtcgacct
      361 gtcggcgacg ttgccgtcgt cgtagggctt gagcacgatc tgggtcgtct tgctgccctt
      421 gttgaaatcc tccagcgtgg ccttggtgat ggcgttgacc tctttgccca cattggccaa
      481 gggcccggtc agcggcacga tctgggcgac ttcaacgcct tgcgccgcat aggcgatgaa
      541 ggattgggcg ggcagggcgt aagagaggag cgcggcaccg gcaacggcgg tcaggaccag
      601 ccaagacaag gaaaagcggg gggtcacgtg gaaacttctg gagaggtttg aaaagggtag
      661 gtctcctccc aatgccgggc gatgtcgatg cggcgacaca cccagacgtt cttatgccga
      721 gaaacatggt ccatgaagcg ctgcagcgcc gtgatgcggc cggggcgacc gagcaggcgg
      781 cagtgcatgc cgatgctcat catcttcggc cgatcaagcc catgcgggtc gccctcggcg
      841 tacagcgcgt caaacgtgtc cttcatgtac tcaaagaacg ggtcggcgtg gctgtagccc
      901 tgcggcagcg caaagcgcat gtcgttgcag tcgagcgtgt agggcaccac caggtgcggc
      961 accacggcgc cgtcggtgcg ctgcaccttc atccagaacg gcagatcgtc gccgtagtag
     1021 tcgctgtcgt acagcaggcg gccgtcgtct gcgaccaggc ggcgcgtgcg cgggctgtcg
     1081 cggccggtgt accagcccag cggccgctcg ccactcagtt tttcaatgat gtccagcgcc
     1141 tcggccatgt gggcgcgttc ggtggcttcg tcgacgttct ggtagtggat ccacttcagg
     1201 ccgtggcagg cgatctcgtc gccgcgctgc tgaaagacct tgaccatttc ggggtagcgc
     1261 gccaatgccg tggcgacgcc aaacacggtg agcggccagc cgcgcgcgtc gaactcgcgc
     1321 aggatgcgcc acacgccggc tcgcgagccg tattcgtaga tgccttccat gctcaggtgc
     1381 cggtcggggt aggccgccgg gttgaacatc tcggacagga actgctcgct ggccgcatcg
     1441 ccgtgcagca cgctgttttc gccgccttcc tcgtagttga ggacgaactg caccgcgatg
     1501 cgcgcgccgc ccggccagcg cgcgtgcggc acgtcggcgc cgtagccgac gaggtcgcgc
     1561 gggtaggcgg cggtgctgtc gtagatcgaa ggcttcatgc tactgaataa atagctactt
     1621 gcgcttgtca gacgggcgcc agggccgatt tgacgtcatg actgggcacc ggctggtcga
     1681 gcttcaggcc ggcctcgaca tggtccaggt gctcgcgcat cagcttgacg gcgcgcgtca
     1741 cgttgccggc agcaaagcac tcgaccagcg cggcgtgctc ggcgctggaa tcgtgcgcgg
     1801 cgtggctgct ctggtacatc agcgtggcga tggcgcaacg cgcgagcagg tcttgcagca
     1861 cgtcggccag cacgtggttg cccagcagtt cggccatcag cacgtggaag tcgcccagca
     1921 gctcggtgcg gccttcgacg tcctggcgtt gcacggctgc ctgttcgcgc gcgacatggt
     1981 cgcgcagggc atcgatgtgc gccggcgtgg cagcggcgac gaaggcgcgc gtgagttcga
     2041 cctccaacat gcggcgcacg gcaaacacct ggcgcgcctc ttcggccgat gggctggcca
     2101 cgaaggcgcc gcgcgccggc tccagcgtga ccaggcggcg ttgcgacagc tgaaacaagg
     2161 cctgtcgcac cagcgtgcgc gacacatcga agtggcccgc cagcgtttgc tcggtcagct
     2221 tggcaccggg ctgcaggcga tgttcgacga tggcgcgggt gagcgctgcg acgatggtgt
     2281 cagtggccga gggttccatt gggcgccgta tcataggtcg aaacgtttaa gaatgtatac
     2341 aattttgtcc acaatttgca tttgtcgagg ccgccgatgg gattgagcac acacgttctg
     2401 gacaccatgc acggcacacc ggcggccggc atggcggtcg ccttgtacga gacgcatggc
     2461 gaggtcgcca cgctggtcaa gcgcttcacc ctgaacgcag acggccgcaa tcccgatggg
     2521 ccgctgtatg acaacgcgag cctgaaggcc ggcacgtatc ggctggcctt cgacgtcgcg
     2581 gggtactttc gcgcgcgcgg cgtgcagttg cccgagccgc catttctgaa cgtcgtgaac
     2641 ctcgacttcg gcatcgccca cgtcgaccag cattaccacg tgccgctgct gtgcagcccc
     2701 tggagttatt ccacctaccg cgggtcttga gcgcgctgac gcgctcaatc tgtttatccg
     2761 cgcgccaccg cgctcaatcc tgcccctcgg tcagcgtgtc cagctgccac tggccgcttt
     2821 tgtgccacag ccaagtgtcg gtgtagacca gcccgcccac cagcgccggc ggcggaaacg
     2881 gatccgccgc ccgcaccatg cgctcaatgg cacgcatcac ttccggcgcg tggcttggcg
     2941 cgcgcatcca gctgatggtg ccgacgcggc cctgaccgtc gatgtgaacc tgcagcacgc
     3001 caacggcata caacagcggc ggcatgcggc ccttgtagat gctgcccagg ttcaggctgt
     3061 agaggtggcg cgccgcgtcc ttgcggtagg cgcgggcggt ggcggcgtcg gacgcctcgg
     3121 gcaccggcac cgggcgcgcc gacgattgac cagcggccgg gcgcacgcgc ttgcgcggct
     3181 tgatcggcgg cgccggcttg cgcgtgctgg ggcgtacggt cttggtgggc gcggctttgc
     3241 tctgcgcctc ggcgggcgtc ggcgcccagg ccagtgcggc gcaggcgatg gtcatggcca
     3301 gcatcaggct gcgaatctgc cagggcctgg ggatcgtgcg atgcggagtg cgcaatgagt
     3361 ctctttcggt gggtgcgctc gttcgcgcgg gcatactgtt gacccggccg caggccttgt
     3421 caatggcttt gtcccagcgc gtgccccaag ttcaggattt attgctgcat cttcggcagg
     3481 ccccggcgct gctggtcagc gtcacgcgca ccgccggctc ggtgccgcgc gaggcgggcg
     3541 cttggctggc cgtgtgcggc gatgcggtca tcggcacgat tggcggcgga caactggagc
     3601 atcaggccat tgcgcatgcg cgcgagctgc tggccggctt ggtaaccgca gcgcacgaaa
     3661 cacggcgcta cccgctgggc cccagcctgg gccaatgctg cggcggcgtg gtggaactgc
     3721 agtacgagca cgtatctgct attgatatgg aagcgctcca cgcacgtctg tcaagcgcca
     3781 caggccattt ggccttgttt ggcggtggtc acgtcgggcg cgccatcgtg cgcctgctgg
     3841 ccgacttgcc gttctcggtg cgctggatcg acagccgcga cgaggtgttt ccacccgacg
     3901 tgccagccaa tgtgcagtgc gagcattccg accccgtgca ggtggccgtg gccgatctgc
     3961 cggcgaatag cgccatcctc atcatgagct tcagccacgc ggaggatctg gacatcgtgg
     4021 ccgccgcgct gacccgccag cgtgcgcgcg gcgatgtgcg ctatatcggc ctcatcggca
     4081 gcaagaccaa gtgggccacg tttcggcatc ggctttcagt gcgcggcttc agcgatgccg
     4141 agttgcaaca ggtgatttgt cccatcggct tgcccgccat ccgcggcaag cggccggcgg
     4201 tgatcgcggt ggcggtggtg gcgcaactgc tggcgctggg cctggctgag ccggcggcct
     4261 gaaacgacaa cggcgcccga aggcgccgtt ggtggataca ggtcagggcg ctgggccccg
     4321 tctgctcaac cctggtgcgg acgcttgccg gggtttttct tgctgcgcgt gtgcgagccg
     4381 cgctcctggc tcttgcgctg gcccacgcct tgcttcgcga cggtggcgcc aggcagcggg
     4441 gcggtggcct tgcgggaggc ttcggtgacg atcttgttgc ccatggtgcg atccttttgg
     4501 ttcgaaagag tttgttgcga ggaaaccgcg tattttcgcc gatttgcgat cgagtgggcg
     4561 gcgtggcgtt tccgcgcacc acgtcgggcg catcccactg gcgtttatgt gccgttttgt
     4621 atacactttt tcctgatcgc agcggtgccg aagcgctggc gatggcaatc aacactgccg
     4681 cgcccgccag atgcgtgatc gcccttgaac ggcttgcagc gcccgcggtg gcgagccttt
     4741 gtagagtaat tccattgctc tgaaggatca cgttcatgga agcctatctg ctcgactggg
     4801 ccaacctgct gctgcgctgg ctgcacgtca tcgtcgccat tgcctggatc ggctcgtcgt
     4861 tctacttcgt ctttctcgac aacaacctgc tggcgcccac cgccgacgac ctgaagaaaa
     4921 agggcgtcaa cggcgccatg tgggcggtgc acggcggcgg tttctacaac ccgcaaaagt
     4981 acatggtcgc gccgcagggc ggcatcatcg attccaagct gcactggttt tattgggaaa
     5041 gctacagcac ttggctgtcg ggctttgcac tgttttgcgt gttgtatctg tggaacgcca
     5101 gcgccttcct ggtcgacaaa cagttgcttg actggtcgcc agtggcggcc ggagtggcgg
     5161 cggtcggctt catggtggcg ttctggttca tctacgacac catctgccgc gtgttcggct
     5221 ttcgcccgaa cggcgagcgc atcgtggcgg cgctgctgat cgtggtggtg gcgctggcga
     5281 cctggctggc gtgccatttg ttctcgggcc gcgcagcctt cctgatcatt ggcgccatgc
     5341 tggcgacctc gatgagcgcc aacgtgttct tctggatcat tcccggccag cgcaaggtgg
     5401 tggccgccat gacctcgggc cagccctacg acgccgatgc gctggccatc cacggcaagc
     5461 gtggtaagca gcgcagcgtg cacaacacgt atttcacatt gcctgtgctg atcgccatgc
     5521 tcagcaacca ctacagcttt ctctacagcc acccgcagcg ctggctggtg ctggtgctga
     5581 tgatgctggc cggcgcgctg atccgccagt tcttcgtgca gcgccacggc tggcaccatg
     5641 gccgcgccag caatccgtgg ccgtttgcgg cagcgggcgt ggtgattttg ctgggcgtca
     5701 tcgcctggct gcggccggcg cctgcaccgg ccgccgcggc agcgccggcc gtggtcgact
     5761 acgcacaact caaccccatt ctggagcagc gctgctactc atgccatggc gaggcggtgc
     5821 agatgaagaa cctgcgcctg gattccgccg attctgtgaa atcgcacgcg cagcagatct
     5881 atcagcaggt ggtggtcacc aagatcatgc ccttgtccaa cgccacgcag atgacggatg
     5941 ccgagcgcca gttggtcgcg cgctggtttg aagggcaggg cagccgaccc tgaggcaaaa
     6001 aagccggcgc tgccggcttg tgatgccatg agacggtggt cagtgcaaga tgatgggacc
     6061 cagcgaactg ctgatcgtct gaccctgccg ctggcgacgt cgatccgtcg ctgcgctcgc
     6121 agcctccggc gcgggcaagg ccaggggcgc tgccagcgcc acgcggacac aatcgcgaat
     6181 gtgctgctca cccaaatagc gcagcgccgt ataccccagc acgcccgaca ccacctgacc
     6241 ggccagcggc acatagcgcg cggcctgcgc cgccgtcagc cgcaccccga ccaggcgtgc
     6301 aaaacggacc accagcgcct tagtcacgag gcgaccggca atcaccgaac cgatcatggc
     6361 gatcgccttt tgcatgcgct ctttctcacg cgcatccagc ttggcaattt ggtgcggcgc
     6421 caggccgaat tcctcgttga tcttgggcag cagctgcgtc agcatggccg cgtccaccgc
     6481 ccaatccagc cccggcagcg gtaccgcacc ggccacgcca gcgaccatcg cgcgcttgcg
     6541 cagcaattgc cggctgcgca acaccgcctg ctccagccgc gtgtggcctt gggccaattc
     6601 cagtgcatcg ggttttgtag ggcgcgccat gtcgaaactc ctcgcgtggt cgggtgatgc
     6661 gctgatcgcc gccccttaac gccgcttggt gaccagaccg tacaggaaca gcacgacgat
     6721 ggcgccgatg atcgacgcga tccagccgac gggcgagccc gccggataca gccccagcac
     6781 cgccccgccg tagctcgcca acagcgcgcc gacgatgccc agaatcgtgg tcatgatcag
     6841 ccccatgctc tggttgcccg gcatgatggc acgcgcgatc agccccacaa tgaagccgac
     6901 gatgatggtg acgatgaggc ccatgttcag ttccttcccg gttggttgca aacaccaggc
     6961 actttaacgc cgcttgcgcg tgattcggtg agctgcggcg caggttgcgg tgttggccta
     7021 cagcagagag agcctgcgga atgccccaag cccgcgcgtc aagcgccgac taacggccga
     7081 tgaggaacac cgcaggcgcc ttgggcgtga cgggccagcg cgcttgccgc cattcggcca
     7141 cgctggcgct gcgcgcgccg tcggcgtctg gcagcgtgag accgctggcc acgcaaaggc
     7201 gcaactggcc atcaagcccg gacagaagcg cttccaacag cacctggttg cgatagggcg
     7261 tctcgatcac gatgcgggtc tggccgcttt gttgcacggc gcggctgatt tgctggattt
     7321 gcgttgtgcg ggcgctggca tcttgcggca gatagccgat aaatgcgaag ttctggccgt
     7381 tcaggccgct ggcggccaag gccagcagaa gcgatacagg tccggtcaga ggcaacactt
     7441 cgatgcccaa ctgatgcgca gcgcgcacca ccgagctgcc cgggtcggcc acggccggca
     7501 tgccggcttc gctgaccagg cccacgtctt ggcccgccag cgccgcggcg agcagcggcc
     7561 gcgcgtccca ggttgcgctg gtctggtggt cgcccttctt gtgcacgtcg cgcggcagct
     7621 cggtgatttg ctgcgtctga atcggttggg ccagcggtac cagcgcatcc acgcgtttca
     7681 gaaatgcgcg ggtggatttg gcgttctcgc cgatccagca agcgatggca gctgcttttt
     7741 tgatagtgcc gagcggcagc acgtcgtcca gcggcgtttg ctctgcgcag ccgtggtcga
     7801 gcggcgtggg caccaaaaac aggcggcctt tcatggcagg cgcagaccgg cggcgcgcag
     7861 caggcgggcg gtgcggatca gcggcaagcc gatcagcgcg gtggggtcgt cgctgaggat
     7921 ggcgtcgagc agcgcgatgc ccagcccctc gcttttggcg ctgccggcgc agtcgtacgg
     7981 ctgctcggcg cgcaggtagc gctcgatctc gtcgtcttcc agcgcgcgaa aacgcacttc
     8041 gacggcggcg agatcgcttt gtttaaagcc tgtcgcctgg cacaccaccg acacggccgt
     8101 ttgaaaaatc accgtttggc cgcgcatggc gcgcaactgc gccaccgcgc gttcgtggtt
     8161 gcccggtttg cccagcggtt ggccggctaa gtcggccacc tgatcggagc cgatcacgat
     8221 cgcctgcgga tggcgacggg cgacgtcgcg agccttggcc aaggccagcc gcacagccag
     8281 atcacgcggc gattcgccct tttgcggcgt ttcgtcgacg cccggcgctt cggtggtgaa
     8341 cggcagcccg aggcgcgtga gcaactcacg ccggtagcgc gaggtcgagc ccagcaccag
     8401 cgcgcgcgcg ctggctgagc cggtggagtc ggtcgtcatg gcgccgattc tcttacactg
     8461 cccgcatgag caagaccttc aaggcgcagc gcctggacgt ggccgcgttc gccgacgcga
     8521 aggcggtatt gagcggcacc gaatcgcttc aaaaatatga gcgacttgcg caggagctgc
     8581 aagcgccagc gcccgatttg accttggatt ggcaagccac gggcggcgcg cgcaaggcgg
     8641 ccaacggcag cgtgtatcca tcggttcatc tgcaggtcca agccacgctg ccgctgacct
     8701 gccagcgctg catgggcgag gtgacggagg cggtcgaggt ggatcggcat tttctgttcg
     8761 tgcccgacga agccacggcc gcggcgctgg acgacgagtc ggacgacgac gtgctggcca
     8821 tcgcgcccga tttcaacctg caggcgctga tcgaggatga ggtgctgatg gcgctgccgc
     8881 tggtaccgcg gcatgaggtt tgcccggatc cagtccgcct gggcgcgcaa accgacgact
     8941 ttgacagtgc gggcgaggcc aagcgccaac cgttcgcggc attggcggcg ctgaaaagcg
     9001 aaaaaaagag cggctgaagc accactggcg gcgcctcaag ctggtgcggg tgccgctttc
     9061 gggctataat catgggcttc gcgcgccaag ctttcaacga aaatcctgag cgcccaagtt
     9121 caccatttga atcgcgccgg cccgtggctg gcagccattc gcccaactcc aaggagccat
     9181 catggctgtt cagcaaaaca agaaatcgcc gtccaagcgc ggcatgcacc gttcgcacaa
     9241 tggtctggca acgcccggca tcgccgtgga gccgaccacc ggcgaaaccc acctgcgcca
     9301 ccacatcagc ccgaacggct tttaccgtgg tcgtcaggtc atcaagtcca agaccgaagc
     9361 ctgattgatg cggcgggcgg cgtgggccaa cggcccgcat gggccgcctg ccacgccgat
     9421 ttatgatggc cgggcccggc gctacgcctc gtagcctcgg gcctttggca ttttcagtgg
     9481 ccttgttttt cgccgcgcca gtgcttgtgc gcggtttttg cctggggctt cttgcatgat
     9541 tgttctggcg gttgactgca tggggggtga ccacggcccc aaggtcacct tgcccgcctg
     9601 ccggcagttt ctggatgctc ggccggacgc gcaactgatg ctcgtcggcc aggccgaggc
     9661 gctggcgggc tttgcgcacg agcgcgcccg cgtggtgccc gcgaccgagg tcgtgaccat
     9721 ggacgacgcc gttgaaatcg cgctgcgcaa gaaaaaggat tcgtccatgc gcgtggcgat
     9781 ccagcaggtg aaagacggcg cggctcaggc ggcggtgtcg gccggaaaca ccggcgcgct
     9841 gatggcgatt gcgcgctacc tgctgaagac gctggacggc atcgagcgcc cggccattgc
     9901 cacgcaaatc cccaacgccc agggcggcgc caccacggtg ctcgacctgg gcgccaacgt
     9961 cgattgcacg gccgaccatc tgctgcagtt cgccgtcatg ggctcggcat tggtgtcggt
    10021 gctcaaagat gatccgcagc cgacggtcgg cctgctcaac atcggcgaag aagcgatcaa
    10081 gggcagcgac gaaatcaagc gtgcgggcga gttgctgcgc gcagccgcgc aggccggcga
    10141 cctgaacttc gtcggcaacg ttgagggcaa cgacattttc aaaggcacgt cggacctggt
    10201 ggtttgcgac ggctttgtcg gcaacgtgct gctcaagacc agcgagggct tggcgacgat
    10261 gatcggccaa ttcttgcgcg aagagttctc acgcaacatc ctgaccaaat tggccgccat
    10321 cgtctcgtat ccggtcttaa aagcgcttaa aaaacgcatg gaccatcgcc gctacaacgg
    10381 cgcggcgctg ctgggcctgc gcggcctggt gttcaaaagc cacggttcgg ccgatgtgat
    10441 ggccttcgag caggcgctgg cgcgcgcgta tgatgccgcc acccaccagc tgctggatcg
    10501 gctgcaaagc cgcatcgccc acgccgcgcc cttgctggca tcgccggcgg cgcagcgcct
    10561 ggatgcggcg gccacgccgg caggctgaag gcgcctaagc ggcacggccg caggcgcgat
    10621 caaaccaaga gacttcatga ccagacgcta ttcccgcatc accggcaccg gcagctacct
    10681 gccgccgcgc cgcgtgacca acgacgatct ggtgactgag ctgggggcgc gcggcatcga
    10741 gacgtccgac caatggatcg tcgagcgcac cggcatccgc gcgcgccact ttgccgagcc
    10801 cgacgtgccg gccagcgacc tggcggtgcc ggcggcgcag gcggcgctgc aggcggcggg
    10861 cctgcaggcg gccgacatcg acctcatcat cgtcgccacg tccacgccgg acatggtgtt
    10921 tccgtcgacc gccgccatcg tgcagcacaa gctcggcatc gccggttgcc cggtgttcga
    10981 cgtgcaggcg gtgtgcagcg gctttgtcta tgcgttgacg gtggccgacg cgctgatcac
    11041 caacggcacg gcgaccaagg cgctggtgat cggcgccgag gtgttcagcc gcattcttga
    11101 tttcaacgac cgcaccacct gcgtgctgtt tggcgatggc gccggcgccg tagtggtgga
    11161 ggcgtcggac acgccgggca ttctggcgac cgacctgcac gccgatggca agcacgtcgg
    11221 cattctgtgc gtgccgggca cggtggccgg cggccaggtg ctgggcgatc cgctgctcaa
    11281 gatggatggt caggccgtgt tcaagctcgc cgtgggcgtg ctcgacaagg ccgcgcgtgc
    11341 cgtgctggcc aaggccggca agagcgaggc cgacatcgac tggctgattc cgcaccaggc
    11401 caacatccgc atcatgcaga gcacggcgaa aaagctgcat ttgccgcagg agaaagtggt
    11461 ggtgacggtg cacgaccacg gcaacacctc ggcggcgtcg attccgctgg cgctcgacca
    11521 cggcgtgcgc gcgggccagg tcaagccggg gcagacggtg ctgctcgaag gcgtgggcgc
    11581 tggcttcacc tggggtgccg tgcttcttat tttatagctg cttgcgcttg actggcaagc
    11641 gctggagccg tttttcattc atagttgcgg gctgaatcat gaaacccatc gcctttgttt
    11701 tcccgggtca gggctcgcaa gccgtgggca tgctggacgc ctggggcgat cacccggcgg
    11761 tgcgcgagac gctggccgaa gcctccgacg cgctgggcga ggacatcggt gcgctgatcg
    11821 ccaatggccc caaggaagac ctggcgctga ccaccaacac gcagcccgtg atgctggtgg
    11881 ccggcgtcgc ctgctggcgc gcctggcggg ccgagggcgg cgctctgcct gccgcggtgg
    11941 ccggtcattc gctgggtgaa tattccgcgc tggtcgccgc cgaggcgctg ccgctggccg
    12001 aagccgtgcc gctggtccgc ctgcgcgcgc aggccatgca ggaagccgtg ccggtgggta
    12061 ccggcgccat ggccgccatc ctgggtctcg aagcttccaa agtgatagct ggctgcgcag
    12121 aagtgacggg cgccatgggc caaaacggca ctgaagtggt cgaagccgtc aacttcaacg
    12181 atccggtgca aacggtgatt gccggcacca aggcggcggt cgaaaaagcc tgcgaagtgc
    12241 tcaagggcat gggcgccaag cgcgcgctgc cgctgccggt gtcggcgccg ttccactcca
    12301 gcctgatgaa gcccgccgcc gagcgcctca agggcgcgct ggaggcggtc gacttcgccg
    12361 acccgaagat cccggtcgtt aacaacatcg atgtggccat cgaaagcgac cacgaccgca
    12421 tccgcgacgc gctgtaccgc caggcgtttg ggccggtgcg ctgggtcgaa tgcatccaca
    12481 agatcaaggc catgggcctg cacaccgtgg tcgaatgcgg gccgggcaag gtgctggccg
    12541 gcatggtcaa gcgcatcgac gccgatttga ccggcctggc cgtttacgac ccggccacgc
    12601 tggccgaggc gaaggcgcag ctcggctgat ttgttattgt tttgatagct gcttgcgctt
    12661 gtccagcaag cgctggcggc acgaaaggca tgaatgtctg aggtgcatgg agcaaaccgc
    12721 gtcgccctgg tcacgggcgc cagccgcggc attggcgccg ccattgcgca agagctggcc
    12781 gcgcgcggct accgcgtcat cggcaccgcc accaccgatg ccggcgccga gaagatcagc
    12841 gccgcgctgg ccgcgcaccc gggctgccgg ggcgccgcgc tcgacgtcaa cgatgcggcg
    12901 gcggccgaag ccttgatcga agccatcgtc aaggaacatg gcgcgctgca cgtgctggtc
    12961 aacaacgccg gcatcacgcg cgaccagctg gccatgcgca tgaaagacga cgactgggac
    13021 gcggtgctgg acaccaacct gaaagccgtc ttccgcatga gccgcgccgt catgcgcccg
    13081 atgatgaagc agcgtcaggg ccgcatcatc aacatcacca gcgtggtcgg cgcctcgggc
    13141 aaccccggcc aggccaacta cgccgccgcc aaggccggcg tggcgggcat gacgcgcgcg
    13201 ctggcgcgcg agctgggcag ccgcggcatc accgtgaact gcgtggcgcc gggcttcatc
    13261 gccaccgaca tgacggccgc gctgggcgac gagcagcaaa aggcgttgac gggccagatt
    13321 ccgctcggcc gcctcggcgc gccggccgac attgctgccg cggtggcgta cttggccagc
    13381 cccgaagccg gctacgtgac gggtcaggag ctgcacgtca acggcggcat gttcatggcc
    13441 tgacggccta cactatttca cccctgccgg ccaccgcccg ggatggcggt aagcgcaggt
    13501 aaaatcaatt tttttatcac cctcagaggg aacccatgag cgatatcgaa gcacgtgtca
    13561 agaaaatcat cgccgagcaa ctcggcgtgg aagagtcgca ggtcacgaac gaaaaggcct
    13621 tcgtggccga cttgggtgcc gactcgctcg acacggtcga actggtgatg gcgctggaag
    13681 acgaattcgg catcgagatt cccgacgagg acgccgaaaa gatcaccacg gtgcaagccg
    13741 ccatcgacta cgccaacagc aagaaggcgt aaggcgcggc gccctcaggc gcccagcgca
    13801 ggcagcggcc agtagggggc tgcgtgccgc cacggccggc cggtagggcc ggctttgttc
    13861 atagaccgtc caccaagatc cgatgagcat gaatcgacgc cgcgttgtgg ttaccggcct
    13921 gggttgcgtc agccccgtgg gcaacacggt ggccgaaagc tgggccagcc tgctggcggg
    13981 gcgctcgggc atcgacctga tcacgcaatt tgacgccagc gcgctggcct gccgctttgc
    14041 gggcgaggtc aagggcttca acgtcggcga ctacatcgcc gaaaaagaag cgcggcacat
    14101 ggatcgcttc atccatctcg ggctggccgc cgccgcgcag gccgtggccg attccgggtt
    14161 gccgacgggt gacgcgctga gctccgatca ggccgagcgc ttcggctgca acatcggctc
    14221 gggcattggc ggcctgccga tgatcgagca gacgcacgcc gaatacgcga gtcgcggccc
    14281 gcgccgcatt tcgccgttct tcgtgccggc ctcgatcatc aacatgattt ccgggcacgc
    14341 ctcgatccgc tttggattca aggggcccaa cattgccatc gtgacggcct gcacctcggg
    14401 cctgcacgcc attggcctgt ctgcacggct gatccagcac ggcgacgccg atgtgatggt
    14461 ggccggcggt gcggaatcca ccgtgtcgcc actgggcgtt ggcggctttg ctgcggcgcg
    14521 cgccttgtcg acccgcaacg acgaccccaa gaccgcgtcg cgcccctggg acaaggaccg
    14581 cgacggcttc gtgctgggcg agggtgcggg cgtcgtggtg ctcgaggaat acgagcacgc
    14641 caaggcgcgc ggcgcgaaga tttacgccga gctgatcggc tttggcatga gcggcgacgc
    14701 cttccacatg accgcgcccg acgtggacgg cccgcgccgc tccatggcca acgcgctgcg
    14761 tgatgcgggc gtcaacgcga acgcggtgca gtacctgaac gcgcacggca cctcgacgcc
    14821 gctgggcgac ctcaacgaaa ccaacgccat caaggcggcg ctgggccatg cggcgcgcca
    14881 ggtcgtggtc agctcgacca agtcgatgac cggccacctg ctgggcggcg ccggcggcat
    14941 cgagagcgtg ttcaccgtga tggcgctgca cgagcagaaa gtgccgccca ccatcaacat
    15001 cttcaaccag gatccggagt gcgacctgga ctattgcgcc aacacggcgc gtgacatgaa
    15061 gatggacgtg gcgctgaaga acaacttcgg ctttggcggc accaacggct cgttggtgtt
    15121 caagcgcgtc tgaggttctg ccgcgtcttg ctcgataggc gcgcgggggt gtgatggatc
    15181 gggcgccttc ggtggtctat cccgtcggtc gcacgcgcgc cctggcttgg gtgctggcgg
    15241 tgctggccgg cagcggtgcc gtgctcttgt tttcagtgct tttttggccg atgacgcccg
    15301 tccagcgtgc gccagaagct attgttttga tagtttcatg gttgggcgcc gtggcagcga
    15361 tgtggcattt ctggcgccgc cagcgcccgc accagttgcg ctgggacggc gaatgctggt
    15421 tcgtcgtcgc gccgaccgat gccgtggagc aaggcggcga gggcgcgcgg gtcgaggtgc
    15481 gctgggatgc gcagcgctgg atgctgctgt ggtttcatcc cggcgctgcc ggcgagcgcg
    15541 ggcggtggct gtgggccgaa gcggcaagcg agcccgcgcg ctggcacctg ctgcgctgtg
    15601 cgctatattt accgctgact tcggcatctg tcggcgctcc gccgtcatcc gaggcaggac
    15661 gcgcctgatg tcgggcgcgt ctttgttttg aggcttgttt tttcttgacc gaccgtcccg
    15721 cagcccctcc cgccccgacc gacagcgacg cgctgctggt cgagcgcgcg gctgcgggcg
    15781 accaccgcgc ctacgagttg ctggtcatca agtaccagcg ccgcatcgag cgcctgatcg
    15841 gccgcatggt gcgcgacgtc gacctggtgc ccgacatcgc gcaggagacc ttcatccgcg
    15901 cctggcgcgc gctgcaccag ttccgcggcg acgcccagtt ctacacctgg ctctaccgca
    15961 tcgccgtcaa cacggccaag aagtcgctga tggagctcaa gcgtgatccg gtgatgaccg
    16021 aatcggcgct gaaatccgtc gatgacgacg atgaaacttc ccggcgcgag aacgaactaa
    16081 cctccgaatc cacgccggaa tcggaactgg cggcccgcga aatcgccgcc gccgtgaacg
    16141 ccgcgatgga ggctttgccc gaggatttgc gccaggccgt cacgctgcgc gagatcgagg
    16201 gcctgagtta tgaagagatt gcggaagcca tgaattgccc gatcggcacg gtgcgctcgc
    16261 ggattttccg ggcgcgcgag gcgatttcag ccagggtcaa gcccttgctg agccaccaga
    16321 caggcaagcg ttggtaaagg tggatgggaa gaaaacggta acggtggtca tcatgaagac
    16381 tcaacttgac attccatgtg atcgtgaaac ggtgtcggcg ctgatggacg ggcagctgtc
    16441 cggcgcgcaa gccgctatgg cggtgcaatc gatggcctcg accgaggcgc gcgaatgctg
    16501 gcatgtctat cacctggtgg gcgatgtgct gcgctccacc gatctggctg cctgcggcca
    16561 cgatgcggcg ttcgtggcgc gcctcgccgc tcggctgcag cacgaaacac cggcgtttga
    16621 ggcgctgcct gctttggcaa ggcccgcaat gctggatgtc gcccgtcccg ccgccaacga
    16681 tggcgtgttc cgctggaagc tggtggctgg cttggcatcg tttgcggctg tggcggccat
    16741 cggctggggt gcgttgggca gcatcggccc gcaacccggc gccggcgcgc aattggcgcg
    16801 cagcgcggcg cccgccgtgg gcacgacgca agtcatggcg ctcgcagcgc cagtgccggc
    16861 ggcctcgcaa gcggccgaca tggccccggt gatgctgcgc gatgcgcgtc tcgacgagtt
    16921 gctggccgcg caccgccaga cggtgggcgc atcggcgctc ggcaacgccg cgggcttctt
    16981 gcgcaacgcc accttcgagg gaccaggacg ttgatcgcgg gcctgtcgtc gggtgatcgt
    17041 gcagccggcg cgcgccgcgt gccggcgcgc gggtggcgtc tgcgcctgcg tcaagcggcg
    17101 gtgatctgtt gcgccggcag ccttgtcacg ctggcgggcg cgcaaaccgg cggctcgttt
    17161 ggcggcggtg cgtccgaacg ccccatcggc ccgccgcaac gcagcatggc ggaatggctg
    17221 gtaaggctgc agcaggcatc gcgtgtgccc tcgtatgtgg gcaccttcgt ggtgtcgtcg
    17281 gcgtcgggcg ccatgtcgag cgcgcgcatc tggcacgtgt gcgagggcga tgtgcagatc
    17341 gagcgcgtcg aagcgctgac cggcgcgccg cgctccacct ttcgtcgcaa cgattcggtg
    17401 atgacgtttt tgccggatgc gcgcgtggtc aagcttgacc aaagcgagtc gggcggcatt
    17461 ttccccaatc tgctgaacat caaccaggct ttcccgacat ccgacttcta cgcctcgcgc
    17521 gaactggggc agggcagggt ggccggtttc gatgccgaca tcgtgatgct gagcccgcgc
    17581 gacgagtggc gctatggcta tcgaatctgg agcgagaagc gcaccgggct ggtggtcaag
    17641 acgcaaaccg ttgatgcggg tggccgcgtg ctggagcagg cggcgttctc tgaattgcaa
    17701 ctcggcgtac cggtgcaggc cgacaagctg cgccagatga tgggcagcac cgaaggctat
    17761 cgggtcgtca aggccgagcg cacgcgcacc acgccggaag ccgaagggtg gcagctgaaa
    17821 aaccccgtgc cgggtttcag gtctgagaac tgctatcgcc gctcggccgc caaagagtcg
    17881 cccttggtgc agtggatttt ttccgatggg ctggtcacag tgtcgctgtt catggagccg
    17941 tttgaccgcc agcgccatca gggcgaaggc gtggcatcca tgggcgccac gcacaccctg
    18001 acgcgtcgcc tggtcgatcg cgcgggcgac tggtgggtca ctgcggtggg tgaggtgcct
    18061 ctgcccactt tgcaggcctt tgtgcagggc ctgcagcggc ggccgtgagc gcggcagtgc
    18121 gatgccgtgc ggcgatgaat caatgagagt ggagagttct atgtcgacga tgagaaagca
    18181 atggggcctg atcgccctgg cgacgccgct gttgttggct ggatcgttgg ccgtgccgac
    18241 gaccgccgcg gcccaggggc cggccgccgt ggcgccttcg gtgcgtggcc tgcccgattt
    18301 caccgatctg gtcgatcagg tcggcccggc ggtggtcaat atccgcacgg tggagcgcgt
    18361 gcgggcaggc gccgccggcc gcggcgggca gatggatcag gaaatgcagg agttcttccg
    18421 gcgtttcttt ggcatcccca tgcccaatac accacgcccg caaggcccac gcgccgatcc
    18481 tcaggagcaa cccgaacgtg aagggccgcg cggtgtgggc tcgggcttca tcatttcggc
    18541 cgatggcttc gtgatgacca atgcgcacgt ggtcgatggc gcggacgaag tgctggtcac
    18601 gctgcccgac aagcgcgagt tcaaggccaa gctcatcggc gccgacaagc ggaccgacgt
    18661 ggcggtggtc aagatcgatt ccacgggctt gcccttcgtg cgcattggcg acatcaatcg
    18721 tctgcgcgtc ggcgaatggg tgatggccat tggctcgccc ttcgggctgg aaaacacggt
    18781 caccgccggc atcgtcagcg ccaagcagcg cgatacgggc gactacctgc ccttcatcca
    18841 gaccgacgtg gccgtcaacc cgggcaactc gggcggcccg ctgatcaaca tgcgcggcga
    18901 ggtggtcggc gtcaacagcc agatctattc gcgctcgggc ggtttcatgg gcatctcgtt
    18961 cgccattccc atcgacgaag ccatgcgcgt gagcgagcaa ctgcgcagca gcgggcgcgt
    19021 cacgcgcggc cgcatcggcg tgcagattgc cgaggtgacg cgcgaggtgg ccgaatccat
    19081 cggtctcggc aagccgcaag gcgcgctggt gcgcagtgtc gagactggat ctccggcgga
    19141 caaggcgggc atcgaggcgg gcgacatcat cacccgcttc gagggtcggg ccatcgagaa
    19201 ggcgtctgac ctgccgcgct tggtgggcaa tgccaagccg ggcagccgcg gcgccatcac
    19261 ggtgtttcgc cgcggccagt cgcgcgacct gaacgtgacg gtggccgagc ttgaaccgga
    19321 gcagacagcc aacgcccggc gcccggccgc gcgcagcagc gagccggcca agccggccgg
    19381 cgccggccag gcgctcggcc tcacggtcac cgatctcacg gacgcgcaga agaaagagct
    19441 gaaagccaag cgtggcgtgc gggtggaggc ggccgccgac gccggtgcgc gcgccggctt
    19501 gcgcgagggc gacgtgattc tttcagtcgc caatgtcgac atccagaacg tgcgcgagtt
    19561 cgaagctgca gtggctaagg ccgaccgcag caagccgatc aatatgctgg tgcagcgggg
    19621 cgaattggtg cagttcgtac tggtccggcc gccccgctga tcgggtcgat ggcaggaaaa
    19681 gccccaaccc gcagtcaggt ttggggcttt tttgcgacgg cttgacgggt cgctctggat
    19741 tcaccgatgg cggtgtgcgc tcctctttgc gaggagctaa taccgatttg gatagaattg
    19801 gcgcctcgaa aatctgttta tctgaatatc gagggccttt gtcaccacca tgtggcacgt
    19861 ttcgcggcaa tccaccggaa gtccacaagc gcatggcaag gtgttcagaa tctatcccat
    19921 cgaaattgtt aataagctgt tgataaatat tcgttgcagc gtcacaacga cgccggcacc
    19981 gcgaacttgg gtcatccgcc gacgcgcttt ttgcctacaa tgaaggccca actatcgcag
    20041 ttgccataga ttgttggttg agggcgcgtc atatcactgg cgcgcccttt tttcatgtgc
    20101 gcttactgct aagtcaaata cttgcctgca ttcgttgatg gaccacatca gaaatttttc
    20161 aatcattgcg cacatcgatc atggcaagtc cacgctggcc gatcgactca tctcgcgttg
    20221 cggcggcctc actgatcgcg agatgagcga gcaggtgctc gactcgatgg acatcgagaa
    20281 ggagcggggg ataaccatca aggcgcagac cgccgcgctg cagtacaccg cacgcaatgg
    20341 acaggtctac aacctcaatc tgatcgacac gccgggccac gtcgacttct cttatgaagt
    20401 gtcgcgctcg ctgtcggcat gcgaaggtgc gctgctggtg gtcgatgcgt cgcaaggggt
    20461 cgaagcgcag actgtggcca actgctacac cgcgctcgat ttgggcgtcg aggtgctgcc
    20521 agtgctcaac aagatggatt tgccgcaggc cgatcctgac aacgccaagg ccgagatcga
    20581 ggacgtgatc ggtatcgacg catcggatgt catcgcgatc tcagccaaga cgggcatggg
    20641 catcgacgac gtgctggagc agatcgtcgc caaggtgccg gcgccgcgtg gccagcccaa
    20701 cgcgccactg cgcgccatga tcatcgattc ttggttcgac tcctatgtgg gcgtcgtcat
    20761 gttggtgcgc gtggtcgacg gccggctgca aaagggtgag cgcttcaaga tgatggccac
    20821 cggcgccgcc tacgaagcca acaacctggg cgtgttcacg ccggccacgc tgccgcgcga
    20881 cgaattgaag gccggcgagg tgggctacat cattgccggc atcaaggaat tgaaggcggc
    20941 caaggtgggc gacaccatca cgctggagaa aaagctgccc aacaacctgg ggccggctga
    21001 gaaggcgctg ccgggcttca aggaaatcca gccgcaggtg tttgccggcc tgtacccgac
    21061 cgaagcgaac cagtacgacg cgctgcgcga ctcgctggaa aaactgcagc tcaacgatgc
    21121 ttcattgcac ttcgagcccg aagtcagcca ggcgctgggc tttggctttc gctgcggctt
    21181 tctcggcctg ctgcacatgg agatcgtgca ggagcggctg gagcgcgagt tcgaccagga
    21241 tctgatcacc accgcgccga gcgtggtcta tcaggtggag aaggccgacg gcgaagtgct
    21301 gatggtggaa aacccatcga agatgcccga ccagggcaag atcaacgaga tccgcgagcc
    21361 catcgtcacc gtgcacctgt acatgccgca ggaatacgtc ggcccggtga tgacgctggc
    21421 caaccagaag cgcggcgtgc agctcaacat ggcctaccac gggcgccagg tcatgctgac
    21481 ctacgagctg ccgctcggcg aaatcgtgct cgacttcttc gacaagctca aaagcgtgtc
    21541 gcgcggctac gccagcatgg actacgagtt caaggaatac cgcccgtccg acgtcgtcaa
    21601 ggtcgacatc ctgctcaacg gcgagaaggt cgatgcgctg tcgatcatcg tgcaccgcag
    21661 ccagagccag taccgcggcc gggccgtggc ggccaagatg cgcgagatca tcagtcgcca
    21721 gcagttcgat gtcgccatcc aggcagcgat cggtgccaac gtgatcgcgc gcgagaacat
    21781 caaggcgctg cgcaagaacg tgctggcaaa atgctatggc ggcgacatca cccgcaaacg
    21841 caagctattg gaaaagcaga aggcaggcaa gaagcgcatg aagcagattg gttcggttga
    21901 ggtgccccag gaagcgttcc tggccattct gcaggtggag gattgatgcc gatcctgacc
    21961 gccatcatcc tcgccgcctt tgtcggctac ggcgcctcat ggtacttcgg cgccatcgag
    22021 ggcaacttcg cgctgctgct gttcatggcc accgtggtca ctggtgtcta ctggctggcc
    22081 gagcgcttca tctttctgcc gcgccgtcag gccgctgccg ctgcgctgca gcagcaggcc
    22141 gaccagcgtc gctccgagtt ggccgcgcag ggcatcacgc aggtcgacga gggcgacatg
    22201 gcgcgcgcgc gggagcgcgt gctggcgcag ccctggtggc tcgactggac ggccgggctg
    22261 ttccccgtga tcctgatcgt gttcgcgctg cgctcgtttg tgttcgagcc gttcaagatc
    22321 ccctccggct ccatgatccc cacgctgctg gtgggcgacc tgatcctggt gaacaaattc
    22381 acctacgggc tgaagctgcc ggtcgtcaac agccgcctga ccgagggcaa tgccgtgcag
    22441 cgcggcgacg tcgtggtttt tcgctatccg ccgcagccga gcgtggatta catcaagcgt
    22501 gtggttggat tgccgggtga cgaggtggcg tatctcaata agcggctgac catcaacggt
    22561 cagccggtgg ccaccaccgc agtctccgac ttcttcgacg agtcgaccat ggagtacttc
    22621 aagcagttcg acgagcaact gggcgtgaaa aatcaccgga tcatcgttga cgagcgccgt
    22681 ccgggctttg tcgccggtgc gttcgacttc cagtacaagg acaattgccg ctacaacgtc
    22741 gagggcgtca cctgcaaggt gcccgagggc cagtatttcg tgatgggtga caaccgcgac
    22801 aattcgctgg actcgcgctt ctggggtttc gtgcccgacc gcaatatcgt cggtaaggcc
    22861 ttcttcgttt ggatgaactt cggcgacctc aagcgcatcg gctccttcaa ttgacctgac
    22921 gataaatcaa aaaatgacgt accaacgctt tcattcccaa cgcgggatct cgttcttcgg
    22981 cctgctgatc gtgggtgtcg tgctggcctt gctggccatc gttggcgcgc gcgtgctgcc
    23041 gaccgcgacc gaattcatgg cgatccagaa agcggccaag aaagcggcgg cggaaggcgg
    23101 caccgtgccg caggtgcgcg ccgcgtttga tcgcacggcc gcggtcgact acatcagctc
    23161 gatttccggc aaggacctgg aggtgacgaa ggacggcgac aaggtcgtgg tgcagttcgc
    23221 ctacgacaag gaaatcccct tggccggccc ggcctacctg ctgctcaagt accgcggctc
    23281 atcggccggt ggctacaact gaacgcgcca ggccacccgg gcccgcggtg agcgcgcccg
    23341 tcaccctgca ggcgctgcaa cagcgcctgc agcaccagtt tgcgaaccag tccctgcttg
    23401 agcaggcact gacgcaccga agcttctcgg ccgaccacaa cgagcgcctg gagtttctag
    23461 gcgattcggt gctcaacctc gcggtggccc acatgcttta ccaggcgctc ggcagcggcg
    23521 ccgagggcga tctgtcgcgc ctgcgcgccc aactggtacg gcaagacagc ctgcaccgcc
    23581 tggcgctgga cttgggtttt gctccgttgc tgcggctggg cgcgggcgag gtgagttcgg
    23641 gcggccagcg gcggccctcc atcctggctg acgcgctcga agcgatcatc ggcgcggtgt
    23701 acctcgacgg tggcccgctg gcggccgacg cgctggtgcg ccgcctgttc gagcgcgtcg
    23761 agatttcccc cgccacctcg gccgccgcca aggacgccaa gaccgccttg caggagtggc
    23821 tgcaggggcg caagatgcgg ctgccgcagt atgaagtggc gcgcgtgctg ggcgccgcgc
    23881 accgccagac tttcgaggtg gtgtgccagg tggccgcgct cggcctggaa gccagcgggc
    23941 aaggcgtgtc gcgccgcgct gccgagcaag ccgccgccgc cgccatgctg cagcaagtgc
    24001 agaataccca gacatgaata ctcctgaaaa aatagctgct gacgctgatc cgacaagcac
    24061 tggcggcccg ttgggcacaa attctccggc gacggggcag cgctgcggcg tgatcgccat
    24121 cgtcggcaaa cccaacgtcg gcaagtccac cctgctcaac gcgctggtcg ggcaaaagat
    24181 cagcatcacc agccgcaagg cgcaaaccac gcgccaccgc atcaccggta tccgcacgct
    24241 gccggatggc gacggcggtg gcacgcagtt catctttgtc gacacgcccg gcttccagac
    24301 tcggcacagc acggcgctca acaagtcgct caaccgcacg gtactgggcg ccatcgacgg
    24361 cgtcgatctg gtgctgttcg tggtcgaggc cggcagcttc accttgggcg acgccaaggt
    24421 gctggcgctg ctgcagccgg gcacgccgac gctgctggtg gccaacaagc tcgacaccgt
    24481 gcaccgccgc gccgaaatcg cgccctggct gcgcgacatg caagagcgcc acccgtttgc
    24541 cgaattcgtg cccatgtcgg ccaagaaccc gaaggacgtg cagcggctgc tgcagatttg
    24601 cgagcgctac ttgcccgagc agccttggtg gtacggccag gacgagctga ccgacaagag
    24661 cgagaaattc ctcgccagcg aggtggtgcg cgaaaaattg ttccgcctga ccggcgacga
    24721 attgccctac acctccaccg tcgtcatcga aaagttcgac gaagagaaaa gcaagcagca
    24781 cggccgcatg gtgcgcattg ccgccaccat cgtggtcgag cgcgacggcc acaaggccat
    24841 ggtgatcggc gagaagggcg agcgcctcaa gcgcatcggc accgaggcgc ggcaggagct
    24901 ggaaaagctc atggattgca aggtgttcct cgaactctgg gtcaaggtgc gctcaggctg
    24961 ggccgacgat gaagcgcgcg tgcgcagctt cggctacgaa taagccaggc gctgcgccca
    25021 tgcgcctctg gacgctgcac ccgcgccacc ttgatccgca gggcctggtc gcgctgtggc
    25081 gcgaagcatt gctggcgcag gctgtgctgt tcggccgcac gcgcggctac acgcgccacc
    25141 cgcaactgca gcgctttgcc gaacacgccg ctccgctgga cgccgtgggc gcctacctga
    25201 cggcggtggc agaagaagca agcgcgcgcg gctatcgctt cgatgcgagc aagatcgatc
    25261 gcccctcggc gcaagccgcg cccatcaccg ccaccaccgg gcagatcgag cacgaatggc
    25321 gccacctggg cgccaagctg gccgcgcgca acaccgactg gcacgcgcgc tggggcgctg
    25381 ccggccagcc agcgacgcac ccgctgttca ccgtggtgcc tggcccggtg caggactggg
    25441 agcgcccgtg aagcgcgtca cgcacgagcc cggctacgtg ctgcaccact acgactggag
    25501 cgaatccagc gtcatcgtcg aagccttcac gcgcgcgcac ggccgcgtgg cgctggtcgc
    25561 caagggcgcc aagcggccgt cgtccaactt ccgtccggtg ctattgcctt tgcagcagct
    25621 tgcgctgagc tggggcggcg atgcggagat tcgcacgttg aaaagcgccg agtggggcgg
    25681 cggccaggtg atgcccacgg gcgatgcgct gctctccggc tactacgcca acgaacttct
    25741 catgcgcctg ctggcgcgcg aagacgccca cccgcgcctg ttcgatgcat acgcggcgct
    25801 ggtgcacgtg ctggcctcgc acgaggccga actgctcgtc gccgccgcgc tgcgcgcttt
    25861 cgagttgttg ctgctgcgcg agaccggcca cctgcccgcg ctcgacgcgc agacactcac
    25921 cttcgccccc ttgagcgacg acgagcgcta ccgactggtg gccgaaggcg gcctgcgcgc
    25981 cgcccacgcc gacgacagcg cctggctgca tggcgcccat tggcgcgcat tgacggccgc
    26041 gctggccgac gaagcgccgc tgtcggccac gctgaacacc gtggccgaac tgcccggcga
    26101 tgcgcgcgcc cagttgcgcc agcagttgcg tgagctgctc cactaccatt gcggcgtcgg
    26161 cacgctgcgc acgcggcagt tgctgatgga catacaggcc ctgtcgtgaa caccaccacc
    26221 cagctctccg tcaacgtcaa caaggtcgcg ctggtgcgca acacgcgtca cctgggcatt
    26281 cccagcgtca cgcgcgcggc cgagctgtgc ctgcaggccg gcgcccacgg catcaccgtg
    26341 cacccgcgcc ccgacgagcg ccacatccgc gccgccgacg tgcgcgagct ggccgcgctg
    26401 atgaagcaat ggcccggccg cgaatacaac atcgaaggca acccgacgca gaacctgatg
    26461 gccttcatcc gcgaagtgcg tccgcagcaa gccaccttcg tgcccgacag cgaagaccag
    26521 ttcaccagcg accacggctg ggacttggcc gacgcagcgg tgcgcgagcg cct
//
LOCUS       DAOMVO010000004        24289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_326_length_24289_cov_13.242841, whole genome shotgun sequence.
ACCESSION   DAOMVO010000004 DAOMVO010000000
VERSION     DAOMVO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 24289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24289
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_326_length_24289_cov_13.242841"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKV30_00530"
     CDS             <1..253
                     /locus_tag="PKV30_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRL28738.1"
                     /translation="LIRMVRQDCGSCHGIQLTGGLGPALTRDRMADFPYDSLVAVIYH
                     GRPGTPMPGWKSMISEADAHWIARQLQTGFPLEADKTAP"
     gene            250..1464
                     /locus_tag="PKV30_00535"
     CDS             250..1464
                     /locus_tag="PKV30_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRL28739.1"
                     /translation="MTRSLTLTPVSRRTVLALSSATVLTACASRPSLLIEPGRGSGDL
                     GLVIERESGSIGVLDTTARTRLGTVSGLGDLSHASVVFSRDGALAYVFGRDGALTKVN
                     LITGRIEGRVMQAGNSIGGAISTDGTLVVAQNYQPGGVKVFDARTLRPLADIPAVVDG
                     KASRVVGLADIPRNRFIFSLFDAGQIWIADLADPTKPVLTQFANIGKQPYDALVTPDG
                     RHYIAGLFGEDGLARIDLWAAQPKVERILPHYGKGEQPLPVFKMPHLRGWSIAGKHAY
                     LPAIGRHEVLVVDTDTWKEVGRIPVAGQPVFVMARPDGRQVWVNFAVPDYDTVQVIDT
                     QTRQIVRSLKPGKAVLHMEFTPRGDAAWISTRDDHKVVVVDTQTFQTLATLPAKSPSG
                     IFFTSRAARTGF"
     gene            1775..2611
                     /locus_tag="PKV30_00540"
     CDS             1775..2611
                     /locus_tag="PKV30_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL28740.1"
                     /translation="MQDAGAISRIGGVWAPGVGGAALLCAMAVPPERLQDVAARVSAH
                     PGVNHNYEREHALNLWFVITGTGAEPLDQALAAIEAETALPVIRLKMVRPYRIDLGFD
                     LHRPASAGRAIQHRPPRVDAADHGLAALVEAGLPLVPEAFADWARALGCGHAEVQARI
                     QGWLGAGTLRRFGLVVRHHEVGFSANAMTVFDIDDDAIDAFGERLATQPGVTLCYRRE
                     RAEGWRYNLYCMVHGRSRDETLALLHAAIAGAGLQHEPRQILFSRRRFKQTGARYFRC
                     TP"
     gene            2885..3196
                     /locus_tag="PKV30_00545"
                     /pseudo
     CDS             2885..3196
                     /locus_tag="PKV30_00545"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            3350..3787
                     /locus_tag="PKV30_00550"
     CDS             3350..3787
                     /locus_tag="PKV30_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL28741.1"
                     /translation="MPLVPRPYEAVAAVLGATSAAVRARLGELLAAGVVRRIAAVPNH
                     YRLGYVANGMSVWDVDDSDVDRLGALIGSQPAVSHCYRRPRKAGVWRYNLFAMLHGHT
                     REEVLAQAEQVAALLGPARRAHDVLFSSAILKKTGLRLRQPIA"
     gene            3969..5243
                     /gene="nirJ"
                     /locus_tag="PKV30_00555"
     CDS             3969..5243
                     /gene="nirJ"
                     /locus_tag="PKV30_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HRL28742.1"
                     /translation="MFRISQFMHELARAEATGSYPPPARRRSEGDGQNSNGAPAGAPG
                     PVVIWNLIRRCNLTCKHCYAFSADHDYPGELSLDEVFGVMDDLKAFGVPALILSGGEP
                     LLRPDIFEIAERAKAMKFYVGLSTNGTLIDEPLARRIHEHGFDYVGISLDGIGATHDK
                     FRRLDGAFDKSLAAVRHLQKLGTKVGLRFTMTELNAHDLPNLLQLMREENVDKFYFSH
                     LNYAGRGNIHRGKDARHIATRAALDLLYDTAWADARDGGQHEYVTGNNDADGVYLLQW
                     VEKNLPRWTENVRARLVAWGGNSSGVNIANIDNVGDVHPDTMWWHYGLGNVKARPFSE
                     IWQDTSDPLMAGLKAKPRPVGGRCGGCAHFAICGGNTRVRAQQVTGNAWAEDPGCYLT
                     DEEIGFHGSGERVETTAYSSRRKVIDLVHLDS"
     gene            5286..6893
                     /locus_tag="PKV30_00560"
     CDS             5286..6893
                     /locus_tag="PKV30_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRL28743.1"
                     /translation="MFFEVFRPWRLAACLLAWGAGAHALTPAATPPSAAPASVQALYQ
                     QHCAACHGAQRLGGMGPALLPESLARLRKPDAIKVIQDGRAATQMLPFKDTLKAEEIS
                     ALTDWIYTPVKPAPTWSDADIVASRIETAGWKNWPAKPQWQADPMNLFVVVEGGDHHV
                     TLLDGDSFEPIHRFSSRFALHGGPKFTPDGRYVFFGSRDGWITKYDLWNLKTVAEVRA
                     GINMRNIAVSGDGQWVMAANYLPHTVVLFDAELKPVKTYTATTLDGKQSSRVSAVYDA
                     EPRKSFVVALKDIPELWEISYDPQAAPIYDGLVHDYKMGEGIAKPGFQGVRRTPLDEP
                     LDDFFFDQSYAHALGAARPHADGSPSGQVVNLDARRKIATLPIAGMPHLGSGITFDWN
                     GTRVLASPNLQDGTISVIDMKTWKLVKNIATPGPGFFMRSHEKTPYAWTDSMMAPKAK
                     HIMTIIDKRTLEPVAEVKGSPGKTLAHIEFTKDGKYALASLWEDDGALIVYDAATFKE
                     IKRLPMRKPVGKYNLHNKISKSEGTSH"
     gene            complement(6989..7687)
                     /locus_tag="PKV30_00565"
     CDS             complement(6989..7687)
                     /locus_tag="PKV30_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRL28744.1"
                     /translation="MDLEKFNLPRYLATLPLFTSMREDELTRLATGCELRRMERGKLI
                     FRTGDPCNEFHVPVLGQVKLFAISPAGTEKVIELCGPGHTFAEAVMFLGVPYVVSAQT
                     LSDTLLLTVDKAVVLREIQFNPDFSLRMLAGLSRRLHGLIKDVEGYALHSGVQRVIGY
                     LLGDRMTESDAASEAITVSLPVSKAAIASRLSLTPEYFSRVLNELETAGLIQVDKRDI
                     HITDTARLANYQPR"
     gene            complement(7747..9729)
                     /locus_tag="PKV30_00570"
     CDS             complement(7747..9729)
                     /locus_tag="PKV30_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRL28745.1"
                     /translation="MEEWVGARWHRFIWQAADVSFPKAAVRLEDVQRAVQMLFRAGGG
                     DAIVRVVPATAQKIGGPRNWLQKVAGQGERAALSTLDEQTLALPPLIAVLPDAALNRD
                     LYLWLAAQAACHHATPAGWLADNVAATQRALVRFPGLRARYARLAQAHLAQRGAPAKL
                     RGSARDQEQRVQEALRASFSPSPAGGGLGWGPPALPGEGAAPIPAFPQRGKEPDSTQP
                     TAIAPVWLWLQALHTTENASESIADDADPASAEKQKRDENPVKQDTKRRRAQRTEDTS
                     NKDGLVMFFRAESILSWSEFVRVNRTGDDEDNQDYTQVADDMEMLHLAHRKPGETLAA
                     RIKFDLDLPSPSADDLPVGEGEPLPEWDEKSARLVPDRCRAQRFRHRAPAANEDAPTP
                     DAGAAMAQRLGSTSRQVRRRFEALRAAPRWQHGLTQGDELDLDAWVRHQVDRRDARQP
                     HGESPAIFSRRVRSERSLSTLMLADLSLSTDAYATHAARVIDLVRDSLYVFGNALDGV
                     GDPFEMLGFSSVRRAVRIHELKRFGEPWNDAARTRVAAIKPGFYTRMGAAVRYGTRSL
                     GERPERQRLLLILSDGKPNDLDHYEGRHGLEDTRHAVLEARQAGLVPFCVTIDQEANQ
                     YLPRLFGAQGFALVHRPDQLARALTQVYIRLTAAVR"
     gene            complement(9734..10543)
                     /locus_tag="PKV30_00575"
     CDS             complement(9734..10543)
                     /locus_tag="PKV30_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HRL28746.1"
                     /translation="MYRTESAATPTIPFYAEQGDEVRLFEHAYRQQLPALLKGPTGCG
                     KTRFVEHMAARLGRPLITVSCHDDLTAADLVGRYLIGDGNTVWNDGPLARAVRTGAIC
                     YLDEVVEARKDTTVVLHPLADDRRQLPIERTGELLQASPEFMLVVSYNPGYQNLLKNL
                     KPSTRQRFVALVFDYPAPEIERRIIVAESGADEKLATRLVKLASALRRLTDQDLEETV
                     STRLLVVAARLVVSGLPELIAARAAIVDALTDDADTAEALNEVVLAAFAED"
     gene            complement(10679..12139)
                     /locus_tag="PKV30_00580"
     CDS             complement(10679..12139)
                     /locus_tag="PKV30_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRL28747.1"
                     /translation="MTHNPILKYQSQAVAKPYFIAAIGLFVGQIIFGVLMGIQYIYGD
                     FLFPAIPFNVARMVHTNLLIVWLLFGFMGAAYYMVPEEAETELYSPKLALVMFWIFLV
                     AGALTIVGYLLVPYAKLAEMTGNNLLATMGREFLEQPLPTKVGIVIVALAMLFNLSMT
                     YLKGRKTTITTVLMLGLWGLALLFLFSFVNPHNLVRDKMYWWWVVHLWVEGVWELIMA
                     ALLAYVLIKTTGVDREVVDKWIYVIIAFALFTGILGTAHHYYFIGLPGYWHLVGNIFS
                     SLEPIPFFLMTMFAFRMVQRRRRDHANQAAVLWAVGCAVMGFLGAGVWGFLHTLSWVN
                     YYTHGSQLTASHGHLAFYGAYVLVVIALISYAMPTLRGRIANSHKAQSLEMWSFWVMS
                     IGMGVMVLAMTGAGILQVWLQRMPTSGAMPFMAAQDQMMFFYWMRLGGGGVFLIGLVM
                     YLWSFFVGGAPAEESLGTPRRGGLMPSPAQRRLKHD"
     gene            complement(12163..12615)
                     /locus_tag="PKV30_00585"
     CDS             complement(12163..12615)
                     /locus_tag="PKV30_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRL28748.1"
                     /translation="MSTNSSGGFTKEMARNIFYGGSLFFILVFVGLVFHTERTIPERS
                     NEAAMTEAVVRGKMVWEQNNCIGCHTLLGEGAYFAPELVNVYQRRGGDASGGAFIKAW
                     MKAQPTGAPGRRQMPQFNLTDGQLDDLVEFLKWSNGINAEKWPPNIEG"
     gene            complement(12845..14392)
                     /gene="paaZ"
                     /locus_tag="PKV30_00590"
                     /pseudo
     CDS             complement(12845..14392)
                     /gene="paaZ"
                     /locus_tag="PKV30_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
     gene            complement(14541..15284)
                     /locus_tag="PKV30_00595"
     CDS             complement(14541..15284)
                     /locus_tag="PKV30_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL28749.1"
                     /translation="MSAPLLTLPKFSVYYGPVQAIHGIELHVGEGEIVTVIGPNGAGK
                     TTLLNAIMGLLPSQGGLTLGGDTIARPTVEAMVRRGVALVPEKRELFGEMSVEDNLLL
                     GGFALWRRGQRDQPARMKEIFAIFPRLEERRAQMASTLSGGERQMLAIGRALMSRPKL
                     LMLDEPSLGLAPLIVREVLNTVSALRKTGVSVLLVEQNARAALNVADRGYVLEMGEVA
                     VQGPADQLIHDQRVIDTYLGVGGARAAAA"
     gene            complement(15281..17095)
                     /locus_tag="PKV30_00600"
     CDS             complement(15281..17095)
                     /locus_tag="PKV30_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HRL28750.1"
                     /translation="MKPQVLVIGFVALLAAVWGFLPPFTVTLLCYIGLYSLVAVGLVM
                     LTGVGGMTSFGQAAFVGLGAYATAWICTAPEAAEWLSFLPKGLYPWVGLVLGLVVTAL
                     IAWGLGAVTLRLSGHYLPLGTIAWGLSLYYIFGNLQALGGFTGISSLPPLEIFGISLA
                     SPRMLGIVIWAVLLLAIWALHNLLDSREGRAIRALKTGRLMAESMGVDTANQRIKLFV
                     LAALLAAVSGWLYAHLQRFVNPTPFNLNIGIEYLFMAVVGGSGHLWGAVLGATLITLL
                     KEQLQDWLPRLLGTSGNFETVVFGLLMLLVLQRASTGLWPLLAGFAQRVFELQPPARA
                     LAAAGGASLDQRQLPPAGEQILKADQVTKRFAGLVANNAVDMDVRAGEVHALIGPNGA
                     GKSTFFNMISGVDDPTEGQVRLMGQVMQGKPSRAFAALGLGRTFQHVRLLGERSVLDN
                     VALGAHRRGHKGWIASMLRADRAEEAAVLAEAMRQIERCGLADVAHTPAASLPLGQQR
                     IVEIARALAGQPAVLLLDEPAAGLRHLEKQSLAKLLMQMRAEGLAILVVEHDMEFVMN
                     LADRITVLEFGTVIAHGTPAEVQANPRVIAAYLGGDLA"
     gene            complement(17092..18120)
                     /locus_tag="PKV30_00605"
     CDS             complement(17092..18120)
                     /locus_tag="PKV30_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL28751.1"
                     /translation="MDLQIALMLVQDGIVNGAIYALMALALVLVFSVTRVIFIPQGEF
                     VAYAALAMAALQSGKLPPLLWMLLGMAALCLVVEAVRQARGLEVDWKSTLFWAVALPA
                     LAALMLLVVKPQAMWLQVVAVLLLVTPMGPLVYRLAFRPLAHATVLILLIVSVALHLA
                     MVGLGLFFFGAEGSRTEAFSEARWDVGGLTITGQSVVILGVTALLVVAMYFFFERTIV
                     GKALRATAINRVGARLMGIPTALSGDLSFALAALIGAVSGLLIAPVTTIYYDTGFLIG
                     LKGFVAAIVGGLSSYPLALAGALLVGQLESFSSFWASAFKEVIVFTLIIPVLWWRSLH
                     THHVEDEE"
     gene            complement(18251..19405)
                     /locus_tag="PKV30_00610"
     CDS             complement(18251..19405)
                     /locus_tag="PKV30_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL28752.1"
                     /translation="MQLKKLALLCAAVAGFSTAAWADVNVGVTVSATGPAASLGIPER
                     NTIALMPREIAGQKINYIVLDDASDTTAAVTNTRKLISENKVDIVLGSTTTPNSLAMI
                     DAVTESKTPMISMAASASIVEPVDAKKRWVFKTPQNDIMMALAIAEHMAASGVKTAAY
                     IGFSDAYGEGWAREFSKALELKKIQLVANERFSRTDTSVTGQVLKIMGAKPDAVLVGG
                     SGTPAALPQKTLKERGYAGKYYQTHGVANADFLRVGGKDVEGTLLPAGPVLVAAQLPA
                     SNPVRKSALEYIDAYEKAHGKGSVSTFGGHAWDAGLLLKAAVPVALNKAQPGTAEFRA
                     ALRDALEGTKNVAGAHGIFNMSPTDHLGLDQRARVMVKIENGTWKYQETK"
     gene            complement(19559..20515)
                     /locus_tag="PKV30_00615"
     CDS             complement(19559..20515)
                     /locus_tag="PKV30_00615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRL28753.1"
                     /translation="MENEVTLDAKTLHWEREAIEKLLMADLRERRAARRWRLVKSLIW
                     MLLFGALIWLLVSERLPGAAPTTPHTAMVAIRGEIADTGEGSAEAILPALRSAMEDDG
                     AKALVLLINSPGGSPVQAGLVHDEILRLRKLHDKPVYTVVEESCASAAYYIAAASDQI
                     FVDKASLVGSIGVLINGFGFVGTLDKLGVERRLMTAGENKGFLDPFSPQSDKHREHAQ
                     KMLDQIHQQFIAVVRKGRGDRLKETPETFSGLFWTGEQAVQMGLVDHLGSVDSVARDV
                     VKAEKLIDYTNKENIAERFAKRFGAAAGQGAVAALRAVPGLR"
     gene            complement(20596..20973)
                     /locus_tag="PKV30_00620"
     CDS             complement(20596..20973)
                     /locus_tag="PKV30_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRL28754.1"
                     /translation="MIDERSDLIPLCNSADLQEGGLAVPFDVVYGGQTLRAFAIRFEG
                     LAHAYLNRCTHVAMELDYQPDRFFDDTGRWLLCATHGAAYAPDTGDCAGGPCRGGLVK
                     VDLSEFDGVVHWRTNHLLQPLAF"
     gene            complement(20999..21661)
                     /locus_tag="PKV30_00625"
     CDS             complement(20999..21661)
                     /locus_tag="PKV30_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRL28755.1"
                     /translation="MNRPRQFDLICFDWDGTLFDSTAIIVRSIQQAVLDVGGAKPTDE
                     AASYVIGMALMPALAHAAPDVPKEKYPLLGERYRHHYLATQHDISLFDGVLPMLADLK
                     GRHHWLTVATGKSRRGLDDVLASRQLHGIFDGSRTADETAGKPDPRMLHELMREFGAD
                     PERTLMIGDTTHDLQMARNAGVASVGVSYGAHEPHDFGALGPRYIAHSVAGLNRWLAE
                     HA"
     gene            complement(21658..22587)
                     /locus_tag="PKV30_00630"
     CDS             complement(21658..22587)
                     /locus_tag="PKV30_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRL28756.1"
                     /translation="MGWLEVDEESAGQRLDNFLIRHLKGVPKTHVYRIIRSGEVRVNK
                     GRAAADTRVAAGDRVRVPPVRVAERSSDAATAPAREFPTLLEDDHLIALDKPAGVAVH
                     GGSGVSFGVIEQLRRARPDARFLELVHRLDRETSGILLIAKKRSALKDLQDQFRERET
                     GKTYLALVLGDWPDKLKVIDVPLRKYLLPDGERRVRVTAKDDPEGMRSISLVKVRERL
                     PGFTLLEVTIKTGRTHQIRVHLASQGHPIAGDDKYGDFERNKALQKQGLKRMFLHAWR
                     LQFTHPADGARVELHAPLPPDLLSFSKHSPQAA"
     gene            23254..>24289
                     /locus_tag="PKV30_00635"
     CDS             23254..>24289
                     /locus_tag="PKV30_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRL28757.1"
                     /translation="MKRMLINATQAEERRLAIVDGQKLLDYEIEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEERHGFLPFKEISRQYFAEGVSPSQARINEVIKEGQELLVQ
                     VEKEERGNKGAALTTFVSLAGRYVVLMPNNPRGGGVSRRIEGEDRAELKEAMDQLEYP
                     KGASIIARTAGIGRTAPELQWDLNYLLKLWDAIDGAAKGGKGAFLIYQESNLVIRAIR
                     DYFNSDIGDILIDTDDVYEQAHQFMSHVMPEQADRVKRYRDNAPLFSRFQIEHQIESA
                     YSRTVTLPSGGAIVIDHAEALVAIDVNSARAIKGGDIEETATRTNLEAADEVARQMRL
                     RDLGGLIVIDF"
BASE COUNT         4207 a         8312 c         7916 g         3854 t
ORIGIN      
        1 gctgatccgc atggtgcggc aggactgcgg ctcctgccac ggcatccagc tgaccggcgg
       61 cctcggcccg gcgctgacgc gcgatcgcat ggccgacttc ccctacgaca gtctggtcgc
      121 cgtcatctac cacggccggc ccggcacgcc catgcccggc tggaaaagca tgatttccga
      181 agccgacgcg cactggatcg cgcgccagct gcaaaccggc ttcccgctcg aagcggataa
      241 aaccgcacca tgacccgttc cctgaccctg acccccgttt cgcgccgcac ggtgttggcc
      301 ctgtcctcgg ccacggtgct gaccgcctgt gccagccggc cgtccctgct gatcgagccc
      361 ggccgcggca gcggcgacct ggggctggtg atcgagcgcg aaagcggcag catcggcgtg
      421 ctcgacacca cggcgcgcac gcgtctgggc accgtcagcg gcctgggcga cctgtcgcac
      481 gcctcggtgg tgttctcgcg cgacggcgcg ctggcctacg tgttcgggcg cgacggcgcg
      541 ctgaccaagg tcaacctcat caccggacgc atcgaaggcc gcgtgatgca ggccggcaac
      601 tcgatcggcg gcgccatatc gaccgatggc acgctggtgg tggcgcagaa ctaccagccc
      661 ggcggcgtca aggtgttcga cgcccgcacg ctgcggccgc tggccgacat cccggccgtg
      721 gtcgacggca aggcttcgcg cgtcgtcggt ctggccgaca tcccgcgcaa ccgcttcatc
      781 ttctcgctgt tcgacgccgg ccagatctgg atcgccgacc tggccgaccc gaccaagccg
      841 gtgctgaccc aattcgccaa catcggcaag cagccctacg acgcactcgt cacgcccgac
      901 ggccgccact acatcgccgg cctgttcggc gaggacggcc tggccaggat cgacctgtgg
      961 gcggcacagc cgaaggtgga gcgcatcctg ccccactacg gcaaaggcga gcagccgctg
     1021 cccgtgttca agatgccgca cctgcgcggc tggtccattg ccggcaagca cgcctacctg
     1081 cccgccatcg gccggcacga ggtgctggtg gtcgacaccg acacctggaa agaggtcggc
     1141 cgcatcccgg tcgccggcca gccggtgttc gtgatggcac ggcccgatgg gcggcaggtg
     1201 tgggtcaact ttgccgtgcc cgactacgac acggtgcagg tgatcgacac gcaaacgcgc
     1261 cagatcgtgc gcagcctgaa acccggcaag gccgtgctgc acatggaatt cacgccgcgc
     1321 ggcgacgcgg cgtggatcag cacgcgcgac gaccacaagg tggtggtggt cgacacgcag
     1381 acgttccaga cgctggccac gctgccggcc aagtcgccga gcggcatctt cttcacgtcg
     1441 cgcgcggcgc gcacggggtt ttagggccgt gccccgcccc gccgcggact tcctccctct
     1501 cctgcacgcg ggagagggcc ggggtgaggg cggcattcct ccctcccccg ctgggggagg
     1561 gttggggtgg gggccagccg cgtcaccaca gccagcgccg caagcccctc acccccaccc
     1621 tctccctctc cccaaagggg cgagggcgca agacctcccc gcccggctgc tcaacaactg
     1681 gcaacgtggc ttccccttgt caaatcgccc gtttgcgcaa gtggggcaag cggaaggcgc
     1741 tactgaaatc gaagtaatcg aaactttccg gcaattgcag gacgccggcg ccatcagccg
     1801 catcggcggc gtgtgggcgc cgggcgtggg cggcgctgcc ctgctctgcg ccatggccgt
     1861 gccgcccgag cggttgcaag acgtggctgc gcgggtctcg gcccaccccg gcgtcaacca
     1921 caactacgag cgcgagcacg ccctcaacct gtggttcgtc atcaccggca ccggcgccga
     1981 gccgctcgat caggcgctgg ccgccatcga ggccgaaacc gccctgcccg tcatccgcct
     2041 gaaaatggtg cggccctacc gcatcgacct ggggtttgac ctgcaccgcc ccgccagcgc
     2101 cggccgcgcc attcagcacc gcccgccgcg cgtcgatgcg gccgaccacg gcctggccgc
     2161 gctggtcgaa gccggcctgc cgctggtgcc cgaggccttt gccgactggg cgcgcgcgct
     2221 gggctgcggc catgccgagg tgcaggcccg catccagggc tggctgggcg ccggcacgct
     2281 gcgccgcttt ggcttggtgg tgcggcacca cgaagtcggc ttttcggcca atgccatgac
     2341 ggtgttcgac atcgacgacg acgccatcga tgcctttggc gagcgcctgg ccacacagcc
     2401 cggcgtcacc ctgtgctacc ggcgcgagcg cgccgagggc tggcgctaca acctgtattg
     2461 catggtgcac ggccgcagcc gcgacgaaac gctggccctg ctgcacgccg ccatcgccgg
     2521 tgccggactg cagcacgagc cgcgccagat tctgttcagc cgccggcgct tcaagcagac
     2581 cggcgcgcgg tacttcagat gcaccccctg agcatgactt cgcttcgctc aatgacctcg
     2641 gcttcgcctt cgatgacggc acggcgcgcc atgactgaag acatgcgccc gaagggcgcg
     2701 tcatcgaagc ctgcaggccg aagtcatttc gtcaaaaaga caccttatgc gccctgacgc
     2761 ccccaccgac ctgctgcgcc tgcccgccgg catcgacctc gacgccgcgc gcctgatcga
     2821 ccgcctgcag cgcgacttgc ctttgaacga tcgcccgttt gccgacgtcg cggctgagct
     2881 gcagatgagc gaaccgcagg tcatcgacca gctcggacgc ctgctgggca gcggcgtgct
     2941 gacgcgcttt ggcccgctgt accacatcga gcgcgcgggc ggccagttca tcctggcggc
     3001 gctgcaggtg ccggatgagc gctacgcgga agtcacgcaa caagtcaacg cgctgcccga
     3061 ggtggcgcac aactaccggc gcgaccacgc gctcaacatg tggttcgtgg tggccgctga
     3121 aacgcccgaa gccgcctggg ccgcctgcga ccaaatcgag gccaccacgg ggctgccggt
     3181 gcacgcgttt ccgaaagagc gcgaatactt cgtcggcctg tacctgcccc tgttgtccgc
     3241 cgcgcccggc gtggctgccg tgccgcctcg cgcaacacca gcgcctgcgc cgcaacccac
     3301 aacgctgacc gacttcgacc gccaactgat cgccgccacg caggccggca tgccgctggt
     3361 gccgcgcccg tacgaggccg tggccgccgt gctgggcgcc accagcgcgg ccgtgcgcgc
     3421 ccggctgggc gagctgctgg ccgccggggt ggtgcgccgc attgccgccg tgcccaacca
     3481 ctaccgcctg ggctacgttg ccaacggcat gagcgtgtgg gacgtggacg attcagacgt
     3541 tgaccgcctg ggcgcgctca tcggcagcca gccggccgtc agtcactgct accgccgccc
     3601 gcgcaaggcc ggcgtgtggc gctacaacct gttcgccatg ctgcacggcc acacgcgcga
     3661 agaggtgctg gcgcaggccg agcaggtcgc cgcgctgctc ggccccgcgc gccgcgcgca
     3721 cgacgtgctg ttctccagcg ccatcctcaa gaagaccggg ctgcggctgc gccagccgat
     3781 tgcctgagcg cttttttcaa gcgtttcagg gctccagcgc ttgccagaca agcgcgagca
     3841 gctcactttt ttatagttct cgcgcagcct gcgtcggccc gcccggcgcc ggaattgacg
     3901 cacatcatgg ccggcgacgt gcagcggcct aagatcagcc catcccagcc agccccgaag
     3961 gccagcccat gtttcgcatt tcccagttca tgcacgagtt ggcccgcgcc gaggccaccg
     4021 gcagctatcc gccgcctgcg cgccgacgca gcgagggcga cggccagaat tcaaatggcg
     4081 caccagcagg cgcgccgggc ccggtcgtca tctggaacct gatccgccgc tgcaacctca
     4141 cttgcaagca ctgctacgcc ttctcagccg accacgacta ccccggcgag ctgtcgctgg
     4201 acgaagtgtt cggcgtcatg gacgacctga aagcctttgg cgtgcccgcg ctcatcctct
     4261 cgggcggcga gccgctgctg cgccccgaca tcttcgagat cgccgagcgc gccaaggcga
     4321 tgaagttcta cgtcggcctg tcaaccaacg gcacgctcat cgacgagccg ctggcaaggc
     4381 gcattcacga gcacggcttc gactacgtcg gcatcagcct cgacggcatt ggcgccacgc
     4441 acgacaagtt ccgccggctc gacggcgcct tcgacaaatc actggccgcc gtgcgccatc
     4501 tgcaaaagct cggcaccaag gtcggcctgc gcttcaccat gacggaactg aacgcgcacg
     4561 acctgcccaa tctgctgcaa ctgatgcgcg aggagaacgt cgacaagttc tacttcagcc
     4621 acctcaacta cgccggccgc ggcaacatcc accgcggcaa ggacgcgcgc cacatcgcca
     4681 cgcgcgccgc gctcgacctg ctctacgaca cggcgtgggc cgatgcacgg gacggcgggc
     4741 agcatgaata cgtcacaggc aacaacgatg ccgacggcgt gtacctgctg cagtgggtcg
     4801 agaaaaacct gccgcgctgg acggaaaacg tgcgcgcccg cctcgtcgcc tggggcggca
     4861 actcgtcggg cgtcaacatc gccaacatcg acaacgtggg cgacgtgcac cccgacacca
     4921 tgtggtggca ctacggcctg ggcaacgtca aggcgcggcc gttctccgag atctggcagg
     4981 acaccagcga cccactcatg gccggcctga aagccaagcc gcgccccgtg ggcgggcgct
     5041 gcggcggctg cgcgcacttc gccatctgcg gcggcaacac gcgcgtgcgt gcgcagcagg
     5101 tgaccggcaa cgcgtgggcg gaagacccgg ggtgctatct gacggacgaa gagatcggct
     5161 ttcacggcag cggcgaacgc gtcgagacca ccgcgtactc atcgcgtcgc aaggtgatcg
     5221 atctcgtgca cctcgattct tgatcaaatc gggcgccagc gcccgatcag caagcgcaag
     5281 cagctatgtt ttttgaagta ttccgcccct ggcgccttgc cgcctgcctg ctcgcgtggg
     5341 gcgccggcgc tcacgcgctg acccctgcgg ccacgccgcc aagcgccgca cccgcctcgg
     5401 tgcaggcgct ctaccagcaa cactgcgctg cctgccacgg tgcgcagcgt ctgggcggca
     5461 tgggccccgc gttgctgcct gaaagcctgg cgcgcctgcg caagcccgac gcgatcaagg
     5521 tgatccagga cgggcgcgcg gcgacgcaga tgctgccgtt caaggacacg ctcaaagccg
     5581 aagaaatctc ggcgttgacg gactggattt acacgcccgt caaacccgcg ccgacctgga
     5641 gcgacgccga catcgtcgcc agccgcatcg agaccgccgg ctggaagaac tggcccgcaa
     5701 agccgcaatg gcaagccgac ccgatgaacc tgttcgtggt ggtcgaaggc ggcgaccacc
     5761 acgtcaccct gctcgatggc gacagcttcg agcccatcca ccgcttttcc agccgctttg
     5821 cgctgcacgg cggccccaag ttcacgccgg acgggcgcta cgtgttcttc ggctcgcgcg
     5881 acggctggat caccaagtac gacctgtgga acctgaaaac cgtggccgag gtgcgcgccg
     5941 gcatcaacat gcgcaacatc gccgtcagcg gggatggcca atgggtgatg gcggccaact
     6001 atctaccgca caccgtggtg ctgttcgacg ccgaactgaa gccggtgaag acctataccg
     6061 ccaccacgct cgacggcaag caaagctcgc gcgtctccgc cgtttacgac gccgagccgc
     6121 gcaagagctt cgtcgtggcg ctgaaagaca tcccggagtt gtgggagatc agctacgacc
     6181 cccaggccgc gcccatctac gacggcctgg tgcacgacta caagatgggc gaaggcatcg
     6241 ccaagcccgg ctttcagggc gtgcgccgca cgccgctgga cgagccgctg gacgacttct
     6301 tcttcgatca aagctacgcc catgccctgg gcgccgctcg cccgcacgcc gacggctcgc
     6361 ccagtggcca ggtcgtcaac ctggacgcgc gccgcaagat cgccacgctg ccgattgccg
     6421 gcatgccgca cctgggctcc ggcatcacgt ttgactggaa cggcacgcgc gtgctggcca
     6481 gccccaacct gcaggacggc accatcagcg tgatcgacat gaagacctgg aagctggtga
     6541 agaacatcgc cacacccggc cccggcttct tcatgcgcag ccacgagaag acgccctacg
     6601 cttggaccga ttcgatgatg gcgccgaagg ccaagcacat catgaccatc atcgacaagc
     6661 gcacgctgga gccggtggca gaagtcaaag gctcgcccgg caagacactg gcgcacattg
     6721 agttcaccaa ggacggcaaa tacgccctgg ccagcctgtg ggaagacgat ggcgcgctga
     6781 tcgtctacga cgcggccaca ttcaaggaaa tcaagcggct accgatgcgc aagccggtgg
     6841 gcaagtacaa cctgcacaac aagatttcga agtctgaagg cacgtcgcat tgagcgtgat
     6901 gcgggacgcc aaaatgctgc gtttttagta gctactcgcg cttgatgggc gggcgccaaa
     6961 ggccgattca acccgagatt gccgagactt accgtggctg gtagttcgcc agccgcgccg
     7021 tgtcggtgat gtggatgtcc cgcttgtcga cctgaatcag tcccgccgtt tccaactcgt
     7081 tcagcacgcg cgagaaatat tccggcgtca gcgacaggcg cgaggcaatc gccgccttgc
     7141 tgacgggcag cgacacggtg atcgcctccg acgccgcatc gctctcggtc atgcggtcgc
     7201 ccagcaggta gccgatgacg cgctgcacgc cgctgtgcag ggcgtagccc tccacgtcct
     7261 tgatgagacc atgcaggcgg cgcgacaggc cggccagcat gcgcagcgag aaatcggggt
     7321 tgaactggat ctcgcgcagc accacggcct tgtcgaccgt gagcagcagc gtgtcgctca
     7381 gcgtctgcgc cgacaccacg tagggcacgc cgagaaacat caccgcctcg gcaaaggtgt
     7441 ggccggggcc gcacagctcg atcaccttct cggtgccggc gggcgagatg gcgaacaact
     7501 tgacctgacc gagcaccggc acgtgaaact cgttgcacgg atcgccggtg cgaaagatca
     7561 gcttgccgcg ctccatgcgg cgcagttcac agccggtggc caggcgcgtc agctcgtcct
     7621 cgcgcatgct ggtgaacagc ggcaacgtgg ccagataacg ggggagattg aacttttcga
     7681 gatccacggc tggcgttggt gggtgcgtgc ccggcggcgc gcaccgaaac agggcaacgc
     7741 ccgagtctat ctgaccgctg cggtgagccg gatgtagacc tgcgtgagcg cgcgcgccag
     7801 ctgatccggc cggtgcacca gcgcaaagcc ctgggcgcca aacaggcgcg gcaggtactg
     7861 gttggcttcc tggtcgatgg tgacgcagaa cggcaccagt ccggcctgcc gtgcctccag
     7921 cacggcgtgg cgcgtgtcct ccagcccgtg gcggccttcg tagtgatcga ggtcgttggg
     7981 cttgccgtcg gacaggatga gcagcagccg ctggcgctcg ggccgctcgc ccaggctgcg
     8041 cgtgccgtag cgcacggccg cgcccatgcg ggtgtaaaag cccggcttga tggcggccac
     8101 gcgcgtgcgg gcggcgtcgt tccacggctc gccaaaacgc ttgagttcgt ggatgcgcac
     8161 ggcgcgccgc accgacgaaa agccgagcat ctcgaacggg tcgcccacgc catccagcgc
     8221 gttgccgaag acgtacaggc tgtcgcgcac caggtcgatg acgcgcgcgg catgggtggc
     8281 gtaggcatcg gtcgagagcg acaagtcggc cagcatcagc gtcgacagac tgcgttcgct
     8341 gcgcacgcgg cgcgagaaga tggcgggtga ttcgccgtgc ggctggcgcg cgtcgcgccg
     8401 atcgacctgg tggcgcaccc aggcgtccag gtccagctcg tctccctgcg tcagcccgtg
     8461 ctgccagcgc ggcgcggcgc gcagcgcctc gaagcggcgg cgcacctggc gcgaggtgct
     8521 gccaaggcgc tgggccatgg cggcgccggc atcgggcgtc ggcgcgtctt cgttggcggc
     8581 gggcgcgcgg tggcgaaagc gctgcgcgcg gcagcgatcg ggcaccaggc gcgcgctttt
     8641 ctcgtcccat tcgggcagcg gctcgccctc gcccaccggc aggtcgtcgg cgctggggct
     8701 gggcaaatcg aggtcgaact tgatgcgcgc ggccagcgtt tcgcccggct tgcggtgcgc
     8761 caagtgcagc atctccatgt cgtcggcgac ctgggtgtag tcctggttgt cttcgtcgtc
     8821 gccggtgcgg ttgacgcgca cgaactcgct ccagctcagg atcgactcgg cgcggaagaa
     8881 catcaccaga ccatccttgt tgctggtgtc ttcggtgcgc tgggcgcggc ggcgcttggt
     8941 gtcttgcttg accggatttt cgtcccgttt ttgcttctcg gcgcttgctg gatcagcgtc
     9001 atcagctatg gattctgaag cgttttctgt cgtgtgaagg gcctgcagcc agagccagac
     9061 gggggcgatg gcggtgggtt gggtgctgtc tggctccttc ccccgctggg ggaaggcggg
     9121 gatgggggcc gcgccttccc caggcaacgc gggtggcccc caccccaatc ctcccccagc
     9181 gggggaggga gaaaaactgg cgcgcaaggc ctcctgcacg cgctgctcct ggtcacgcgc
     9241 ggagccgcgc agcttggcag gcgcgccgcg ctgcgccagg tgtgcctgtg ccagccgcgc
     9301 ataccgcgcg cgcaggcccg gaaagcgcac caacgcgcgc tgcgtggcgg ccacgttgtc
     9361 ggccagccag ccggcgggcg tggcgtggtg gcaggcggct tgcgcggcca gccacaggta
     9421 cagatcgcgg ttgagcgcgg catcggggag cacggcgatc aacggcggca acgccagcgt
     9481 ctgctcgtcc agcgtgctga gcgccgcgcg ctcgccctgc ccggccacct tctgcagcca
     9541 gttgcgcggg ccgccgatct tctgcgccgt ggcgggcacc acgcgcacga tggcgtcgcc
     9601 gccgccggca cgaaacagca tttgcaccgc gcgctgcacg tcttccagcc gcacggcggc
     9661 tttcggaaag ctcacatccg cagcctgcca gatgaagcgg tgccagcggg caccgaccca
     9721 ctcttccacc gcttcagtcc tcggcgaacg ccgccaacac gacctcgttc agcgcctcgg
     9781 cggtgtcggc gtcgtcggtc agcgcatcga caatggcggc gcgggcggcg atgagttcgg
     9841 gcaggccgct caccaccagc cgcgccgcga ccaccagcaa acgggtgctg acggtttctt
     9901 ccagatcctg atcggtcagg cgccgcaggg ccgaggccag cttgacgagc cgcgtcgcca
     9961 gtttttcatc ggcacccgat tcggccacga tgattcgacg ctcgatctcc ggcgccgggt
    10021 aatcgaacac cagcgccaca aagcgctggc gcgtgctggg cttgaggttc ttcagcaggt
    10081 tctgatagcc ggggttgtac gaaaccacca gcatgaattc cggcgaggcc tgcagcaact
    10141 cgcccgtgcg ctcgatcggc aactggcgcc ggtcgtcggc cagcgggtgc agcaccaccg
    10201 tggtgtcctt gcgcgcttcg accacttcgt caaggtagca gatcgcgccg gtgcgcacgg
    10261 cgcgcgccag cgggccgtcg ttccacaccg tgttgccgtc gccgatcagg tatcggccca
    10321 ccaggtcggc cgccgtcaag tcgtcgtggc agctcacggt gataagcggg cggcccagcc
    10381 gcgcggccat gtgctcgaca aaacgcgtct tgccgcagcc ggtgggaccc ttgagcagcg
    10441 ccggcagttg ctggcggtag gcatgctcaa acaggcggac ttcgtcgccc tgctcggcgt
    10501 agaagggaat ggtcggcgtg gccgcggatt cagttcggta catgttcatg gccttgcgca
    10561 atgacgaaat gacttcgccg ctggggcggc ttcaatgacg acatgacatt tcggtcatgc
    10621 ggcgaagccg cgtcatcaaa gccgccccag cggcgaggtc atctcgtcat gcagacgatc
    10681 agtcgtgctt gagcctccgc tgcgccgggc tgggcatcag gccgccacgg cgcggcgtgc
    10741 cgagcgattc ctcggccggc gcgccgccga caaagaagct ccacagatac atcaccaggc
    10801 caatcaggaa cacgccaccg ccgcccaggc gcatccagta gaagaacatc atctggtctt
    10861 gcgcggccat gaagggcatg gcgccgctgg tgggcatgcg ttgcagccac acctgcagga
    10921 tgccggcgcc ggtcatggcc agcaccatca cgcccatgcc gatgctcatg acccagaatg
    10981 accacatctc cagcgactgc gccttgtggc tgttggcgat gcgcccgcgc aaggtgggca
    11041 tggcgtagct gatcagcgca atcaccacca gcacgtacgc cccgtagaag gccaggtggc
    11101 cgtgcgatgc cgtcagctgc gagccgtgcg tgtagtagtt gacccacgac agcgtgtgca
    11161 ggaagcccca gacgccggcg cccaggaagc ccatgaccgc gcagcccacg gcccacagca
    11221 cggcggcctg gttggcgtgg tcgcgccggc ggcgctggac catgcggaag gcgaacatgg
    11281 tcatcaggaa gaacggaatc ggctccagcg acgagaagat gttgcccacc aggtgccagt
    11341 agcccggcag accgatgaag tagtagtggt gcgccgtgcc cagaatgccg gtgaacagcg
    11401 cgaaggcgat gatcacgtag atccacttgt cgaccacctc gcggtcgacg ccggtggtct
    11461 tgatcagcac gtaggccagc agcgcggcca tgatcagctc ccacacgcct tccacccaca
    11521 ggtgcaccac ccaccaccag tacatcttgt cgcgcaccag gttgtgcggg ttgacgaagc
    11581 tgaacaggaa cagcagcgcc aggccccaca ggccgagcat cagcaccgtg gtgatggtcg
    11641 tcttgcggcc cttcaggtag gtcatgctga ggttgaacag catggccagc gccacgatga
    11701 cgatgcccac cttggtcggc agcggctgct caagaaactc gcgccccatg gtggccagca
    11761 ggttgttgcc ggtcatctcg gccagcttgg catagggcac cagcaggtaa ccgacgatgg
    11821 tcagcgcgcc ggccaccagg aagatccaga acatgacgag cgccagcttg gggctgtaca
    11881 gctcggtctc ggcctcttcc ggcaccatgt agtaggccgc ccccatgaag ccgaaaagca
    11941 gccacacgat gagcaggttg gtgtgcacca tgcgcgccac gttgaacggg atcgccggga
    12001 acaaaaagtc gccgtagatg tactgaatgc ccatcagcac gccgaagatg atctgtccga
    12061 cgaacaggcc gatggccgca atgaagtacg gcttggccac cgcttgcgac tggtatttga
    12121 gaatggggtt gtgggtcatg gtggtttctc ctgcgggccc gatcagcctt cgatgttggg
    12181 cggccacttt tcggcgttga tgccgttgga ccacttcaga aactcgacca ggtcatcgag
    12241 ctgcccatcc gtcaggttga actgcggcat ctggcggcgg ccgggcgcgc cggtgggctg
    12301 cgccttcatc caggccttga tgaacgcccc gcccgaagcg tcgccgccac ggcgctggta
    12361 cacgttgacc agctcgggcg caaagtaggc gccctcgccc agcagcgtgt ggcaaccgat
    12421 gcagttgttc tgctcccaaa ccatcttgcc gcgcacgacc gcttcggtca tcgcggcttc
    12481 gttggagcgc tcgggaatcg ttctttcggt gtgaaacacc agtccgacaa aaaccagaat
    12541 gaaaaacaat gacccgccat agaagatgtt gcgcgccatc tccttggtga agccgccgga
    12601 tgagttcgtg ctcatgaccg attcctcgct cgctgtgggt agtgataacc cgtatagggt
    12661 agaaaattga ctcaggtcag tccttgaagc aagtcaaggt tgacctaacg atatagtcgg
    12721 gaattcggcc tgatgccaaa acgccgacgc ggccctttca tgcccaactt gaatataaaa
    12781 aatgcctctg acgcttgtct gacaagcgcg agcagctata aaaattgaag tatcagctgg
    12841 ccgtttcgcc cgtcacggcc gccagcatgg ccggcgagcc ctgcagcgcc gcgcgctgca
    12901 tcaggtgctt gacggcgcgc aggccgccca gctcttcgcc gccgccggct cggcccgggc
    12961 cgccgtgctt caggtacggc agcgccgagc cgtggccggt ggattcgggc gccgcggcgc
    13021 ggtcgagaat ttgcaggcgg ccgtgcgtgg cggccatcga cgtgatggcc ttgaccgccg
    13081 tggccacatc cttgatcacc agcgtgccgg ccaggctgcc cttgccgcgc gcggcgagtt
    13141 gcagcgcatc gtccaggtcc tgatacgcca tcagcgtgct gaccgggccg aaggcctcga
    13201 catcgtgcgc agcgtggttt tgcgcgtcct tggccagcag cagcgtgggc tggaagaagg
    13261 cgccttcggc cacgccctcg cccaccggcg caaagccggc gtcgccgccg aacaccactt
    13321 cagcgccttg gcggagcgtg gccacgcgct cggccacgtc gctttgctgc gatttggagg
    13381 ccagcgcgcc catgcgcacg ttctccaggc gcggatcgcc caccgtggtc ttggccaggc
    13441 gctctttcag cttggcggcg acggcgtcga tgcggtcggc cgggaccagc gcgcggcgga
    13501 tggcggtgca tttttggccg gctttttgcg tcatctcgcg cgcgatttcc ttgacgaaca
    13561 gatcgaactc ctcgtcatcg ggcgtcacgt cgggcgccag cacggcgcag ttgagcgaat
    13621 cggcctcggc gttgaacggg atgctgtgct tgatgaggtt cggcgtggcg cgcagcttgg
    13681 ccgccgtgtc ggccgagccg gtaaaggtga ccacgtcttg cccctccacg cggtccagca
    13741 gatcgcccgt gccgccgatg accagttgca gcgcgccttc gggcaggatg cccgattcgc
    13801 tgatcaggcg caccagcttt tcggtcaaga agctggtggc cgtggccggc ttgccgatac
    13861 agggcatgcc ggccagaaag gtgggcgcga atttctccag caaaccccag acgggaaagt
    13921 tgaacgcgtt gatgtgcacc gccacgccac cgcgcggcac cagcacgtgg gtgccggcga
    13981 aggcgccggt cttgccgagc ggcatctgcg ggccctcgtg ccacaggttg ccggccctgg
    14041 gcagttcctt ggccatgctg gcgtaggcaa agagggtgcc tgtgccgcct tcgatgtcga
    14101 cccacccatc ggtgcgcgtg gcgccggtgt gaaaggacag cgcgtacatc tcctccttgc
    14161 gctcgcccag aaacttggcc agggccttga gcatgccggc gcgctgctgg aagtcggtct
    14221 tcagcatggc cggcaggccg acgcggcgcg cgtagtccag cgcctcggca aagtcgatgg
    14281 cgtcggcatg cgtgtgcgcc acggtcgatc catcgatggc gcttttcagc gcctgcgcgg
    14341 gctgcgtgcc gatccagcgg ccggcgatgt ggctttggag ttgaggggtc atgggctgct
    14401 ccttgagggt tgtgccgacg cggctcgcgc caggccacgg acttgaggtg ctgaaatgca
    14461 ggctcagcat cttttaggcc acgagcgctt gtgtatcaag cgcaagcagc tatcaaaatt
    14521 aaagcggcac gcgccgcagc ctatgcggcc gcagcgcgcg cgccaccgac gcccagatag
    14581 gtgtcgatca cgcgctgatc gtgtatcaac tgatcggccg gcccctgcac cgcaacttcg
    14641 cccatctcca gcacatagcc gcgatcagcc acgttgagcg cggcgcgcgc attctgctca
    14701 accagcagca ccgacacgcc ggtcttgcgc agcgccgaca cggtgttgag cacttcgcgc
    14761 acgatcagcg gcgccaggcc gagcgagggc tcgtcgagca tcagcagctt gggccggctc
    14821 atcagcgcgc ggccgatggc cagcatctgc cgctcaccgc ccgacagcgt ggacgccatc
    14881 tgcgcgcggc gctcttccag gcgcgggaag atggcgaaga tttccttcat gcgcgcaggc
    14941 tggtcgcgct ggccgcgccg ccacagggca aagccgccca gcagcaggtt gtcttcgacc
    15001 gacatttcgc cgaacagctc gcgcttttcc ggcaccagcg ccacgccgcg ccgcaccatg
    15061 gcctcgaccg tgggccgcgc gatggtgtcg ccgcccagcg tcaagccgcc ttgcgagggc
    15121 aacaggccca tgatggcgtt cagcagcgtg gtcttgccgg cgccgttggg gccgatgacg
    15181 gtgacgattt cgccctcgcc cacgtgcagc tcgataccgt ggatggcctg caccgggccg
    15241 tagtagaccg agaacttcgg cagggtcagc agcggcgcgc tcatgccagg tctccgccaa
    15301 ggtaagccgc gatcacgcgc gggttggcct gcacctcggc cggcgtgccg tgggcgatga
    15361 cggtgccgaa ttccagcacg gtgatgcggt cggccaggtt catcacgaat tccatgtcgt
    15421 gctccaccac cagaatcgcc aacccttcgg cgcgcatttg catcagcaac ttggccagac
    15481 tttgcttttc aaggtggcgc aggccggcgg cgggttcatc gagcagcagc accgccggct
    15541 gcccggccag cgcgcgggca atttcaacaa tgcgctgctg ccccagcggc agcgacgcgg
    15601 cgggcgtgtg cgccacatcg gccaggccgc agcgctcgat ctggcgcatg gcttcggcca
    15661 gcacggccgc ttcttcggcg cggtcggcgc gcagcatgct ggcgatccag cccttgtggc
    15721 cgcgccggtg cgcgcccagc gccacgttgt cgagcacgct gcgttcgccc agcaggcgca
    15781 cgtgctggaa ggtgcggccc agccccagcg cggcaaacgc gcgcgagggc ttgccctgca
    15841 tgacctggcc catcagccgc acctgacctt cggtcgggtc gtcgacgccc gaaatcatgt
    15901 tgaagaaggt gctcttgccg gcgccgttgg ggccgatcag cgcgtgcacc tcgccggcgc
    15961 gcacgtccat gtcgaccgcg ttgttggcca ccaggccggc aaagcgcttg gtgacctggt
    16021 cggccttcag gatttgctcg cccgcgggcg gcagctggcg ctgatccagc gatgccccac
    16081 ccgccgccgc caaggcccgc gcaggcggct gcagctcgaa cacgcgctgc gcaaaacccg
    16141 ccagcagcgg ccacagcccg gtcgaggcgc gctgcagcac cagcagcatc aagaggccaa
    16201 acaccacggt ctcgaagttg ccgctggtgc ccagcaggcg cggcagccag tcctgcaact
    16261 gctctttcag cagcgtgatc agcgtggcac ccagcaccgc gccccacagg tggcccgagc
    16321 cgccgaccac ggccatgaac aggtattcaa tgccgatgtt gaggttgaac ggcgtcgggt
    16381 tgacgaagcg ctgcaggtgc gcatacagcc agcccgagac tgccgccagc agcgccgcca
    16441 gcacgaacaa cttgatgcgc tggttggcgg tgtcgacacc catcgattcg gccatcaggc
    16501 gcccggtctt gagcgcgcgg atggcgcggc cctcacgcga atccagcagg ttgtgcagcg
    16561 cccagatggc cagcagcagc accgcccaga tgacgatgcc aagcatgcgc ggcgaggcca
    16621 gcgagatgcc gaaaatctcc agcggcggca ggctgctgat gccggtgaag ccgcccagcg
    16681 cctgcaaatt gccgaagatg tagtacagag acaggcccca ggcgatggtg cccagcggca
    16741 ggtagtgccc cgacagccgc aacgtgaccg cgcccaaccc ccaggcgatc agcgccgtga
    16801 caaccagccc cagcaccagc ccgacccacg ggtacagccc cttgggcagg aaggacagcc
    16861 actcggccgc ctcgggcgcg gtgcagatcc aggccgtggc gtaagcgccc aggccgacaa
    16921 acgccgcctg cccgaacgag gtcatgccgc ccacgccggt cagcatgacc aggcccacgg
    16981 cgaccagcga atacaggccg atgtagcaca gcagcgtgac ggtgaacggc ggcagaaagc
    17041 cccacaccgc cgccagcagc gcaacaaagc cgatgaccag gacctgcggc ttcattcctc
    17101 gtcctccacg tggtgcgtgt ggagcgagcg ccaccacagc accgggatga tcagcgtgaa
    17161 cacaatcact tccttgaacg cgctggccca gaacgatgag aacgattcca gctgcccgac
    17221 cagcagcgcg ccggccagcg ccagcgggta gctcgacaaa ccgcccacga tggcggcgac
    17281 gaagcccttc aggccaatca ggaagccggt gtcgtagtaa atcgtcgtca ccggcgcgat
    17341 cagcaggccg gagacggcgc cgatcaaagc ggccagcgca aaactcagat cacccgacag
    17401 cgccgtggga atgcccatca accgcgcacc cacccggttg atggccgtgg cgcgcagcgc
    17461 cttgccgacg atggtgcgct cgaagaagaa gtacatcgcc accaccagca gcgccgtgac
    17521 gcccaggatg acgaccgatt ggccggtgat ggtgaggccg ccgacgtccc agcgcgcttc
    17581 agaaaacgcc tcggtgcgcg agccttcggc gccgaagaaa aagaggccca acccgaccat
    17641 ggccaggtgc agcgccacag acacgatcag caggatcagc accgtggcgt gcgccagcgg
    17701 gcgaaacgcc agccggtata ccaacggccc catgggcgtg accagcagca gcaccgccac
    17761 cacctgcagc cacatggcct gcggcttgac caccagcagc atcagcgccg ccagcgcagg
    17821 cagcgccacg gcccagaaca gcgtgctttt ccagtccact tccagcccgc gcgcctggcg
    17881 caccgcctcg accaccaggc acagcgcggc catgccgagc agcatccaca acagcggcgg
    17941 cagcttgccg ctctgcagcg ccgccatggc cagcgccgcg taggcgacga attcaccttg
    18001 cgggatgaag atgacgcggg tgaccgaaaa caccagcacc agggccagcg ccatgagcgc
    18061 gtagatggca ccgttgacga tgccgtcctg caccagcatc agggcgattt gtaagtccat
    18121 gcaagccgca gcgcgcgcaa ggcgctatga aattaaaagc aaccgcgccg agaagaaggc
    18181 cggcgctgcc ccgatcaagc tgaaccagcg gcgcgccaga taaaaccagc gcgccgcagc
    18241 ggagtcgagc ttacttagtt tcctggtact tccaggtgcc gttttcgatc ttcaccatca
    18301 cgcgcgcgcg ctggtccagg cccaggtggt cggtcggcga catgttgaag atgccgtgtg
    18361 cgccggccac gttcttggtg ccttccagcg catcgcgcag cgccgcgcgg aactccgccg
    18421 tgccgggctg ggccttattc aacgccaccg gcacggccgc cttgagcagc aggcccgcat
    18481 cccaggcgtg accaccaaag gtcgacacac tgcccttgcc atgcgctttt tcgtaggcat
    18541 cgatgtactc cagcgccgat ttgcgcaccg ggttgctggc cggcagttgc gccgccacca
    18601 gcaccgggcc ggcgggcagc agcgtgcctt cgacgtcctt gccgcccacg cgcaggaagt
    18661 cggcgttggc cacgccgtgc gtctggtagt acttgccggc gtagccgcgc tctttcagcg
    18721 ttttctgcgg cagcgcggcg ggcgtgcccg agccgccgac cagcaccgca tcaggcttgg
    18781 cgcccatgat cttcagcacc tggccggtga ccgaggtgtc ggtgcgcgaa aagcgctcgt
    18841 tggcgaccag ctggatcttc ttcagttcca gcgccttcga gaactcgcgc gcccaaccct
    18901 cgccatacgc atcggaaaag ccgatgtacg ccgccgtctt gacgcccgac gccgccatgt
    18961 gctcggcaat cgccagcgcc atcatgatgt cgttctgcgg cgtcttgaag acccagcgct
    19021 tcttggcgtc gaccggctcc acgatcgagg cgctggccgc catcgagatc atgggcgtct
    19081 tcgactcggt taccgcgtcg atcatggcca gcgaattggg cgtggtggtg gagcccagca
    19141 cgatgtcgac cttgttctcg gaaatcagct tgcgcgtgtt ggtcacggcg gccgtggtgt
    19201 ccgaggcgtc gtcgagcacg atgtagttga tcttctggcc ggcgatctcg cgcggcatca
    19261 gggcgatggt gttgcgctcg ggaatgccca gcgacgccgc cggcccggtg gccgacaccg
    19321 tcacgcccac gttcacgtcg gcccaggcgg ccgtcgaaaa tccagccaca gcggcgcaca
    19381 gcaacgccag tttcttcaat tgcatgggta gtctccccgg ggaatggatg gtgggaatgc
    19441 gcaggcgact gcgcgcctga gattcataaa ctgaccgctc ggtcggttaa tgattctatc
    19501 ggcgcgcaag ccgccgccgc ccgcgatccg ctcgggagtt tccctagctg caaaagcctc
    19561 agcgcaggcc cggcaccgcc cgcaaagccg ccacggcccc ctgccccgcc gccgcgccaa
    19621 agcgcttggc aaagcgttcg gcgatgtttt ccttgttggt gtagtcgatc agcttttcgg
    19681 ccttgacgac gtcgcgcgca accgagtcga cactgcccag gtggtcgacc aggcccattt
    19741 gcacggcttg ctcgccggtc cagaacaggc cgctgaacgt ctccggcgtt tccttcagcc
    19801 gatcaccacg ccccttgcgc accacggcga tgaattgctg gtgaatctgg tccagcatct
    19861 tctgcgcgtg ttcgcggtgc ttgtcggact gcgggctgaa agggtcgaga aagcccttgt
    19921 tctcgcccgc cgtcatcaaa cggcgctcga cgcccagctt gtccagcgtg ccgacaaagc
    19981 caaagccgtt gatgagcaca ccgatgctgc cgacgagact cgccttgtcg acgaagatct
    20041 ggtcgctggc ggcggcgatg tagtaggcgg ccgaggcgca gctttcttcc accacggtgt
    20101 aaaccggctt gtcgtgcagc ttgcgcagcc gcaggatttc gtcgtgcacc aggccggcct
    20161 gcaccgggct gccgccgggc gagttgatca gcagcaccag cgccttggcg ccgtcgtctt
    20221 ccatggcgct gcgcagcgcc ggcaggatgg cttcggcgct gccttcgccg gtgtcggcga
    20281 tctcgccgcg aatggcgacc atggcggtgt gcggcgtggt gggcgcggcg ccgggcagcc
    20341 gctccgacac cagcagccag atcagcgcgc cgaacagcag catccagatg aggctcttga
    20401 ccaagcgcca gcggcgcgcg gcgcgccgct cgcgcaggtc ggccatcagc agtttttcga
    20461 tcgcctcgcg ttcccagtgc agggtcttgg cgtccagggt gacttcattt tccatagctg
    20521 tcagcgcaat ccagataagc ggtggcggtc attatcgtcc tcgttcgtga ccgatcaagc
    20581 agcgggcgag ggttttcaga aagccagcgg ttgcagcagg tgattggtgc gccagtgcac
    20641 cacgccatcg aactcgctga gatcgacctt gaccagcccg ccgcggcacg ggccgccagc
    20701 gcagtcgccg gtgtcgggcg cgtaagcggc gccatgcgtg gcgcacagca gccagcggcc
    20761 ggtgtcgtcg aaaaagcggt cgggctggta gtccagctcc atcgccacgt gcgtgcagcg
    20821 gttgagatag gcgtgcgcca agccctcgaa acggatggcg aaggcgcgca gcgtctggcc
    20881 gccatagacg acgtcgaacg gcaccgccag gccgccttcc tgcaagtcgg cgctgttgca
    20941 aagcggaatc aggtcggagc gctcatcaat catgcgcagg cagctatcga aaagtgagtc
    21001 aggcgtgttc ggccagccag cgattcaggc cggccaccga atgggcaatg tagcgcgggc
    21061 cgagagcgcc gaagtcgtgc ggctcgtgcg cgccgtagct gacgccgacg ctggcgacgc
    21121 cggcgttgcg cgccatctgc aaatcgtgcg tggtgtcgcc gatcatcagc gtgcgctcag
    21181 ggtcggcgcc gaattcgcgc atcaactcgt gcagcatgcg cggatcgggt ttgcccgccg
    21241 tttcgtcggc cgtgcgcgag ccgtcgaaga tgccgtgcag ctggcgcgag gccagcacgt
    21301 cgtccagccc gcgccggctc ttgccggtgg ccaccgtcag ccaatggtgg cggcctttca
    21361 ggtcggcgag catgggcagc acgccgtcga acaggctgat gtcgtgttgg gtcgcgaggt
    21421 aatggtggcg gtagcgctcg ccgagcagcg ggtatttttc tttcggcacg tcgggcgcgg
    21481 cgtgcgccag cgcgggcatc agcgccatgc cgatgacgta ggacgcggct tcgtccgtgg
    21541 gtttggcgcc acccacgtcc agcaccgcct gctggatgct gcgcacgatg atggcggtgg
    21601 aatcgaacag ggtgccgtcc cagtcgaagc agatcaggtc gaattggcgc ggacggttca
    21661 tgcagcttgc ggggaatgct ttgaaaaaga gagcaaatcg ggcggcagcg gggcatgcag
    21721 ctcaacgcgc gcgccgtccg ccgggtgcgt gaactgtagc cgccacgcgt gcaagaacat
    21781 gcgcttcagg ccctgctttt gcaacgcctt gttgcgctcg aagtcgccgt atttgtcgtc
    21841 gcccgcaatg ggatgacctt ggctggccag gtgcacgcgg atctggtgcg tgcggccggt
    21901 ctttatggtc acttcaagca atgtgaagcc gggcaggcgc tcgcgcacct tgaccaggct
    21961 gatggagcgc atgccttcgg ggtcgtcctt ggccgtgacg cgcacgcggc gctcgccatc
    22021 aggcagcagg tatttgcgca acggcacgtc gatgactttc agcttgtcgg gccagtcgcc
    22081 cagcaccagc gccagatagg tcttgccggt ctcgcgttcg cggaactggt cttgcagatc
    22141 tttaagcgcg ctgcgctttt tggcgatgag caggatgccg ctggtttcgc ggtcgagccg
    22201 gtgcaccagt tccaaaaagc gcgcatctgg ccgcgcgcgg cgcaattgct cgatgacgcc
    22261 aaagctaacg cccgagccgc catgcaccgc cacgccggcg ggtttgtcga gggcgatcag
    22321 gtgatcgtct tccagcagcg tggggaattc gcgcgccggc gcggtggcag cgtcggacga
    22381 gcgctccgcc acgcgcaccg gcggcacgcg cacgcggtcg ccagcggcca cgcgcgtgtc
    22441 agcagccgcg cgccccttgt tcacgcgcac ctcgcccgag cggatgatgc ggtagacgtg
    22501 ggtcttgggc acgcctttca ggtggcggat caggaagttg tcgagccgct ggccggcgct
    22561 ctcttcatcc acctccagcc aacccacctc gggtttgccc tgaggcccgg cgctatcggc
    22621 tttggcacct ataatgcgat tcactggcaa ccggccgtaa gtggttgaat ttgaaatgga
    22681 agtcaccgcc tgaatgaaca ggtagtctat tccacccgtc cgcaagcggc tgccggtaaa
    22741 aaggtcgatg ccacgtctgc acaagaaagc gccccgcccc cacgaatcgg gcaggcccgc
    22801 caagaaaagc gcaggccctg aggccgacgc aatgaaacac tgaatacgga attccccatg
    22861 accggcaggc cgcgccccca gcgggccagg cctgcgggtc ggatcgaagc gagacacacg
    22921 tggtgctgat ggcaatgatg atggttctga aacgctggct gcggcttgcg tttttcaagc
    22981 caatgacgcc gccagcgccc gccaggcaag cgcaagcagc tacgttttat gtagtgtttg
    23041 cggtgcctgc acccctcgcc cccatcctcg ccagcccgcc tgaccccggc cgctgaacca
    23101 cccgttcagc ggcatgaagg cgcgcgcacc gcagcgcctt acgcctcagt cagtcgtcca
    23161 aggcagttcc cgccgtttcg ttgcgccgtc cacggcatct tggactccgt tcgcggagtc
    23221 gttgactgct tttgatttga aggacaacgc atcatgaaac ggatgctgat caacgccacg
    23281 caggccgaag agcgccgcct ggcgatcgtc gatggacaga aactgctcga ttacgagatc
    23341 gagattgaag ggcgcgaaca gcgcaagggc aacatctaca aggccgtggt gacgcgcgtc
    23401 gagccgtcgc tggaagcctg ttttgtggac tacggtgaag agcgccacgg ctttttgccg
    23461 ttcaaggaaa tctcgcgcca gtactttgcc gaaggcgtgt cgcccagcca ggcgcgcatc
    23521 aacgaggtca tcaaggaagg ccaggaactg ctggtccagg tcgagaagga agagcgcggc
    23581 aacaagggcg cggcgctgac gaccttcgtc agcctggccg gccgctacgt ggtgcttatg
    23641 cccaacaacc cgcgcggcgg tggcgtcagc cgccgcatcg agggcgaaga ccgcgccgag
    23701 ctgaaggagg cgatggacca gctcgaatac ccgaaaggcg ccagcatcat cgcccgcacc
    23761 gccggcatcg gccgcaccgc gcccgaactg cagtgggacc tcaactacct gctgaagctg
    23821 tgggacgcca ttgacggcgc cgccaagggc ggcaagggcg ccttcctgat ctaccaggag
    23881 agcaacctgg tgatccgcgc catccgcgac tacttcaaca gcgacatcgg cgacatcctg
    23941 atcgacaccg acgacgtgta cgagcaggcg caccagttca tgagccacgt gatgcccgag
    24001 caggcggacc gcgtgaagcg ctaccgcgac aacgcgcctc tgttctcgcg ttttcagatc
    24061 gagcaccaga ttgaaagcgc ctacagccgc acggtgacgc tgccctcggg cggcgccatc
    24121 gtgatcgacc acgccgaagc gctggtggcg atcgacgtca actcggcgcg cgccatcaag
    24181 ggcggcgaca tcgaagaaac cgccacccgc accaacctcg aagccgccga cgaggtggcg
    24241 cgccagatgc gcctgcgcga cctgggcggc ctgatcgtga tcgacttca
//
LOCUS       DAOMVO010000005        24145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_327_length_24145_cov_16.364633, whole genome shotgun sequence.
ACCESSION   DAOMVO010000005 DAOMVO010000000
VERSION     DAOMVO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 24145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24145
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_24145_cov_16.364633"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PKV30_00640"
     CDS             <1..1334
                     /locus_tag="PKV30_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRL28758.1"
                     /translation="QVLNAKQHAREADIVAQAGRPKMITIATNMAGRGTDIVLGGNVE
                     KAVEALEADESLDDTTRRARIDALRAQWQKDHEAVKAMGGLRIIATERHESRRIDNQL
                     RGRSGRQGDPGSSRFYLSLDDALMRIFAGDRVRAIMDKLKMPEGEAIEAGIVTRSIES
                     AQRKVEARNFDVRKQLLEYDDVSNDQRKVIYQQRNDILDAEELAAQIAGLREGCFMDL
                     VRQYVPAESVEEQWDIPALEKVLAEEWQIELPLQKQIESASAITDDDIVETVVKAAND
                     AYAAKLALVGPENFRQFERAVLLQAIDTHWREHLSALDYLRQGIHLRGYAQKQPKQEY
                     KREAFELFGQLLDVVKNEVTRTLMNVRIQSGDQVEQAAEEMEQRGERITNVTYTAPTE
                     TGEAETLPELQVRTGALLGGAAAAAVPRVGRNDPCPCGSGKKYKQCHGALS"
     gene            1479..2708
                     /gene="argJ"
                     /locus_tag="PKV30_00645"
     CDS             1479..2708
                     /gene="argJ"
                     /locus_tag="PKV30_00645"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRL28759.1"
                     /translation="MPVNLSAPVASHLQAVPGVRIGVAEAGVRKAGRKDLTVMLLDAG
                     TSVGGVFTSNRFCAAPVQICRERLQGRPDIRALMINTGNANAGTGEDGLARARQTSAA
                     LAALLKIEPAQVLPFSTGVIMEPLPVERIEAGLPAAIADAAPDHWARAAEGIMTTDTV
                     PKAFSAQAKVGGAEITITGISKGAGMIRPNMATMLGFLATDARVDQSVMHQLALELAE
                     GSFNRVTIDGDTSTNDSFIVIATNQAANAPVTSLDSADGQALKQALLGVARLLAQAIV
                     RDGEGATKFITVQVDGGKTAAECRQVAYAIAHSPLVKTAFFASDPNLGRILAAVGYAG
                     IEDLDQTRIDLHLDDVHVAVNGGRNPAYREEDGQRVMKQSEITVRVNLGRGTASDTVW
                     TCDLSHDYVSINADYRS"
     gene            2765..3640
                     /locus_tag="PKV30_00650"
     CDS             2765..3640
                     /locus_tag="PKV30_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL28760.1"
                     /translation="MTENLARLIDKAERLMARIESVLPQPLSAPDWSQSVAWRYRKRA
                     SGHGALEPVRHVGAMALSDLKEIEPQKEKIERNTRQFVEGRPANNVLLTGARGTGKSS
                     LIKACLNAYAPQGLRLIEVDKADLTDLPDIVDVVAGLPEKFIVYCDDLSFEEGEAGYK
                     ALKSILDGSVAASTPNVLVYATSNRRHLLPEYMKENLSYTHTDDGEVHPGEVVEEKIS
                     LSERFGLWVSFYPFSQDEYLAIVAQWLGALGATPAQIESSRAESLVWALERGSRSGRV
                     AYQFARDFVGRNDAP"
     gene            3762..4226
                     /locus_tag="PKV30_00655"
     CDS             3762..4226
                     /locus_tag="PKV30_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL28761.1"
                     /translation="MTDVLKADAHIQRQAVGERPVVDVAVGVLLQADGRFLLTSRPAG
                     KVYAGYWEFPGGKLEAGESVAEALARELHEELGLVIAPSDVQAWREQLVDYPHALVRL
                     HFCKVHAWRGDLQMREGQSFTWSGLPVQVAPVLPGALPVLEWLQAERLLLDK"
     gene            complement(4282..4503)
                     /locus_tag="PKV30_00660"
     CDS             complement(4282..4503)
                     /locus_tag="PKV30_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRL28762.1"
                     /translation="MSNPARAPNSAKPRTVTCPACGGPSLFTPDNHFRPFCSERCKLH
                     DLGAWANESFRVQVEEDDGAEPSEATRLQ"
     gene            complement(4500..5255)
                     /gene="zapD"
                     /locus_tag="PKV30_00665"
     CDS             complement(4500..5255)
                     /gene="zapD"
                     /locus_tag="PKV30_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HRL28763.1"
                     /translation="MILYEYPFNERIRTYLRLEHLFGRLGQLLARDAPLDHHFALITI
                     FEVMDVTSRADLKSEVLKDLEKQKAHIGAYRGNPAISEASLDAILTQLDDAFQKLNQQ
                     LGKPGHELTDNEWLMSIRSRAVIPGGTCGFDLPAYHAWQQGQPAARRADLQAWAASLA
                     PMAQAITLLLQILRDSGKPQKVMATAGQFQQTLPQGRTFQLLRLRIDESMQLIPEISG
                     NRLMASVRLMRQDADGKLHIAREDAPFEMALCA"
     gene            complement(5281..5895)
                     /gene="coaE"
                     /locus_tag="PKV30_00670"
     CDS             complement(5281..5895)
                     /gene="coaE"
                     /locus_tag="PKV30_00670"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868017.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRL28764.1"
                     /translation="MGAATLLGLTGGIGSGKSTVAQMLAAHGAAVIDADAISRATTAA
                     GGAALPSIARQFGAHLIAPDGALDRTAMRALVFDDADARQRLEAIIHPLVAQETDRQA
                     RLALDAGRRLLVFDVPLLVESGARWRAKVDRVLVIDCDSATQIDRVMARNALPRDQVE
                     QIVAAQASRAQRLAAADLVIFNGAGVTQDALRAQAARLAQTFGL"
     gene            complement(5907..6749)
                     /locus_tag="PKV30_00675"
     CDS             complement(5907..6749)
                     /locus_tag="PKV30_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRL28765.1"
                     /translation="MPEGLWLEVALGGVFGLLIGSFLNVVIYRLPKMMERQWAAECAE
                     MTGQAPPTQEKFNLLVPRSRCQKCGHQIRWYENVPVLSYLFLRGKCSACATPISPRYP
                     LVELVTGLLFAWCVWHWGWTLTAAAWCVFAAVLVALTLIDWDTTLLPDDLTLPLLWLG
                     LLAAASGITGVRLGDAVWGAAAGYLSLWLVYWGFKLATGKEGMGFGDFKLFAALGAWF
                     GWQALVPIILMASVIGALVGIGMKLMSSLREGGVVPFGPFLSMAGLTAMIFGPQSILR
                     VVGL"
     gene            complement(6749..7972)
                     /locus_tag="PKV30_00680"
     CDS             complement(6749..7972)
                     /locus_tag="PKV30_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRL28766.1"
                     /translation="MATQVAGAKGVKEFVFEWEGKDRNGKIVRGETRAGGENQVQATL
                     RRQGIFATKIKKRRMRAGKKIKPKDIAIFTRQLATMMKAGVPLLQSFDIVARGNPNPS
                     VTKLLNDIRADIETGTSLSAAFRKYPLYFNSLYCNLVEAGEAAGILESLLDRLATYME
                     KTEAIKSKIKSALMYPIAVIVVAFVVVSVIMIFVIPAFKEVFSNFGADLPAPTLFVIA
                     MSEFFVQWWWLIFGGLGFGIYFFMQAWKRNEKVQMFMDRLLLRVPIFGDLIEKSVVAR
                     WTRTLSTMFAAGVPLVESLDSVGGAAGNSVYKIATDKIQQEVSAGTSLTAAMTNANIF
                     PSMVIQMTAIGEESGALDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVILGTIIGGI
                     VVSMYLPIFKLGQVV"
     gene            complement(8012..9751)
                     /gene="pilB"
                     /locus_tag="PKV30_00685"
     CDS             complement(8012..9751)
                     /gene="pilB"
                     /locus_tag="PKV30_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HRL28767.1"
                     /translation="MAVADPISKAAAPAVLPGVGRALVMAGKVSQQTADDVFRKAKAA
                     NRNFITELTESGAISASDLAHTMSQAFSAPLLDVDALDVQRLPGDLLDPKLSATYRIL
                     ALSKRGGRLIVVTADPSDHEAAEKVKFATQMGVEWVIGEFDKLSRLIEETTKTATQAM
                     DSIVGGDFEFDEASLGTDGAAEETEAPQTDVEDAPVVKFLHKMLVDAFNMRASDLHFE
                     PYEHNYRVRYRVDGELREIVSPPVAIKEKLASRIKVLSRLDISEKRVPQDGRMKLKIG
                     PDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPIEKERLLKAIERPYGMI
                     LVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVA
                     LKAFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIA
                     PFNIASSVILITAQRLARRLCAHCKAPVDIPRETLMEAGFSELELDGSWTPYKPVGCS
                     ACNNGYKGRVGIYEVMPISEELQRIILADGSALEIAEQARREDVRSLRQSGLHKVKLG
                     VTSLEEVLACTNE"
     gene            complement(9893..9969)
                     /locus_tag="PKV30_00690"
     tRNA            complement(9893..9969)
                     /locus_tag="PKV30_00690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9933..9935),aa:Pro,seq:cgg)
     gene            complement(9995..10924)
                     /locus_tag="PKV30_00695"
     CDS             complement(9995..10924)
                     /locus_tag="PKV30_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRL28768.1"
                     /translation="MQEVDATILRRLESGVPLVGQVASYIISAGGKRLRPVLLLLMCG
                     ALGCRDAQRFNLAAVVEFIHTATLLHDDVVDESTLRRGRATANEVFGNPASVLVGDFL
                     YSRAFQMMVESQNMQIMQVLANATNIIAEGEVLQLMNMHDASLDENAYLHVIRSKTAK
                     LFEASCELAAILAGSSPVTRAACAEYGQSLGTAFQVIDDVLDYDGDAAEMGKNLGDDL
                     REGKATLPLILAMKLGNDADARIVRQSIESGSTDELSRVVDIVRRTGALDGTRAAAAA
                     QAERAIAAARALPANHYTDSLLELAAQLLQRRA"
     gene            11175..11504
                     /gene="rplU"
                     /locus_tag="PKV30_00700"
     CDS             11175..11504
                     /gene="rplU"
                     /locus_tag="PKV30_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRL28769.1"
                     /translation="MYAVIKTGGKQYRVAAGEKIKVEQIAADVGQEITFDQVLAVGNG
                     GDLQIGAPLVSGASVVAKVVAHGKHDKVRIFKMRRRKHYQKRQGHRQGYTELEIGTIS
                     GAGKSKE"
     gene            11511..11771
                     /gene="rpmA"
                     /locus_tag="PKV30_00705"
     CDS             11511..11771
                     /gene="rpmA"
                     /locus_tag="PKV30_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRL28770.1"
                     /translation="MAQKKGGGSTRNGRDSKPKMLGVKVFGGQTITAGAIIVRQRGTR
                     FHAGANVGVGKDHTLFALVDGQVSFATKGALNKQVVLVTPAA"
     gene            11850..12917
                     /gene="obgE"
                     /locus_tag="PKV30_00710"
     CDS             11850..12917
                     /gene="obgE"
                     /locus_tag="PKV30_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRL28771.1"
                     /translation="MKFVDEAFIDIAAGDGGNGCVSFRHEKYKEFGGPNGGDGGRGGH
                     VFAVADPNLNTLVDFRYSRRHEAKRGQHGMGSDMFGAAGDDITLKMPVGTLINDAETG
                     ETLYELLTPGEKIMIAKGGDGGFGNMRFKSAINRAPRQKTPGWPGEKKSLKLELKVLA
                     DVGLLGMPNAGKSTFIAAVSNARPKIADYPFTTLHPNLGVVRVGPEQSFVVADIPGLI
                     EGASEGAGLGHQFLRHLQRTRLLLHMVDIAPFDEGVDPVAQAKAIVAELKKYDKALYD
                     KPRWLVLNKLDMVPVEERAARVKDFVKRFKWKGPVFEISALTREGCEPLVQAIYRHIR
                     TEQDAAAPEAAPDPRFAPEQE"
     gene            12972..14117
                     /gene="proB"
                     /locus_tag="PKV30_00715"
     CDS             12972..14117
                     /gene="proB"
                     /locus_tag="PKV30_00715"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRL28772.1"
                     /translation="MDNSTSEVLRQARRIVIKVGSSLVTNEGRGLDEAAIGEWSRQMA
                     LLAGEPGARREVVMVSSGAIAEGMKRLGWPKRPREVAELQAAAAVGQMGLAQMYETKL
                     REQGLRSAQVLLTHADLADRERYLNARSTLLTLLQYQVLPVINENDTVVTDEIKVGDN
                     DTLGALVANLIEADLLIILTDQKGLYTADPRSDPAAEFVHEARSGDLALERMAGGAGS
                     SIGKGGMLTKILAAKRAAASGASTVIAWGREPDALLRLSRGERIGTLLVAPTQKLQAR
                     KQWIADHLQLRGALVVDEGAVAKLRGAGTSLLPIGMTRVEGVFARGDVVAIRDAAGAE
                     VARGLANYSSAEARLLCRKPSSEINALLGYMAESEMVHRDNMVLSRQ"
     gene            complement(14161..14685)
                     /locus_tag="PKV30_00720"
     CDS             complement(14161..14685)
                     /locus_tag="PKV30_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HRL28773.1"
                     /translation="MRRLLAHAWRAVAIALAALTLPAMAQYADLDRADWREDTVPPPP
                     AYSTSKLIDIEMPRSSSVRMGIDPETISINHETGIVRYVVVARGPSAVNASYEGIRCA
                     TGEYRVYARQVQGNPWSPSGENDWKSMRGQTSIMVQHPFRLARDGICIGTGVRQTVAD
                     MVRELKSGNKTLYY"
     gene            complement(14814..15335)
                     /locus_tag="PKV30_00725"
                     /pseudo
     CDS             complement(14814..15335)
                     /locus_tag="PKV30_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804289.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            15446..17191
                     /locus_tag="PKV30_00730"
     CDS             15446..17191
                     /locus_tag="PKV30_00730"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRL28774.1"
                     /translation="MKASQFLISTLKEAPADAEVLSHQLMTRAGMIKKLGAGIYTYMP
                     MGLRVIRKVEAIVREEMNRAGAIECVMPVIQPGELWTESGRFQTMGPELLRVRDRHER
                     DFVVQPTSEEAVTDIARQDLRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
                     YSFDRDEASAKLSYQKMAAAYRAIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYSTGSDYAANMEKAEALAPAGARPAPGQPMTKTPTPGKSTCADVAELLALPLTR
                     TVKSLVLATEELNDAGEVAKVQIWLLLLRGDHDMNEVKVGKVSGLDQGFRFASVPEII
                     DHFGTKPGYLGPIGLKKPVKIVADREVAAMADWVCGANEEDFHITGVNWGRDLPEPDL
                     VADIRNVVAGDAAPDGRGQLAIERGIEIGHIFYLGTKYSRAMDATFLDDDGKPKPFEM
                     GCYGIGITRLPAAAIEQNHDERGIIWPDAIAPFTVVICPVGMDRSEGVKAAAEGLYAE
                     LMQAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDKGLKDGQLEYQHRRDTAATKV
                     PAGEIAAFVKGRLAA"
     gene            17254..17841
                     /locus_tag="PKV30_00735"
     CDS             17254..17841
                     /locus_tag="PKV30_00735"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRL28775.1"
                     /translation="MLVLPGSLLFHEQSSAGGQLEEPLADSVRSALSSAIANSAPPVP
                     TFATTEARLSYLRWLSSMSDRLYSRVKDFPTRIELLQTIWYESRRAGLDTSMVLGLIQ
                     VESAFRKFAVSSVGARGYMQIMPFWTRVIGDGDPGKLFHMQTNLRFGCVILRHYLDRE
                     RGDLFMALGRYNGSRGKPPYPNAVFAAQKKWVFAG"
     gene            complement(17881..19182)
                     /locus_tag="PKV30_00740"
     CDS             complement(17881..19182)
                     /locus_tag="PKV30_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRL28776.1"
                     /translation="MSFTQSLLIIVLLIAASAFFSVAEISLAASRRLRLRQLADDGDA
                     RALRVLRVQEQPGFYFTAVQIGLNAIAILGGIVGEGLLSPYLAAALKNWLPERTAATL
                     AFAGSVAAVTSLFVVFADLVPKRLGMAEPERVAMAVVGPMERFLLVFKPIVWLYTRCT
                     DVIMRLLGLPTQRDDRITPDDILALAEAGTQAGVLDRPEQQVIENVFELDTRTVTSAM
                     TPRDRIAWLRRDATDEEIRLLIAAEPFSTYPVCDGGLDQLLGYVDAKDLFQRVLKGTP
                     IRLTDEGLLHKLVVVPDSLSLSEVLLQFREQHEDFALIVNEYSTVVGVVTLNDVMSTV
                     MGELVTPEDEDLIVRRGDGSWLIDGHTPMQDVQRALGLDDLPGQGEYDTLAGFLMMML
                     RRVPRRTDSVTVNEHRFEVMDVDNYRIDQVLVTRAEPPSAE"
     gene            19420..20493
                     /locus_tag="PKV30_00745"
     CDS             19420..20493
                     /locus_tag="PKV30_00745"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HRL28777.1"
                     /translation="MFDVLIREAATRFGLGDKALPVAQMLLAYMTNRDTGGLAGFLEK
                     FKAANLGPIVQSWLGGGPSAQPISNSQVETVLGGTGGLLSLITSRLAVNRDNVTAAIG
                     YLLPAIVGRLTPGGSVPATVPPEVASLAAAGQSLLAAPSTAAAAAPAAGGGMMKWLPW
                     VIVAAAALIGLSYCGKKTPAPDATVPPAASAPAEPAAVPPPVTPASEPPKASFGGGAP
                     MAPASAPAEPASTVAAPADEPAGSAVVSLIDANTMPALKVYFDSGKTDVRADFAAKSA
                     DLVAYMKANPDAKAVISGFNDPTGNAAANAELSKNRAQAVQAALVAAGVAQDRTVLEK
                     PAETTGTAASNAASRRVEVVLRK"
     gene            complement(20588..20914)
                     /gene="grxD"
                     /locus_tag="PKV30_00750"
     CDS             complement(20588..20914)
                     /gene="grxD"
                     /locus_tag="PKV30_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRL28778.1"
                     /translation="MDQNTQQRIDDLVKQNDIVLFMKGNASFPMCGFSGRAIQILKAS
                     GVEPAALTTVNVLEDEAVRQGIKEYSQWPTIPQLYVKGEFVGGSDIMMEMYESGELQQ
                     LVTGNA"
     gene            complement(20944..21324)
                     /locus_tag="PKV30_00755"
     CDS             complement(20944..21324)
                     /locus_tag="PKV30_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide prediction"
                     /protein_id="HRL28779.1"
                     /translation="MAALGARWPFVHGVLEVQGGLLTRGCRRLPAPMRFDAITIGHVI
                     LAMDGTCLEAVRAHEHVHVRQYERWGALFVPAYLASSAWQRWRGGHAYLDNRFEREAY
                     ALAPCHAVALAGQPRADNGTGLVE"
     gene            complement(21487..>21594)
                     /locus_tag="PKV30_00760"
     CDS             complement(21487..>21594)
                     /locus_tag="PKV30_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HRL28780.1"
                     /translation="VAPKYRDPATGQTWTGRGKAPKWIDGKDRSKYLIA"
     assembly_gap    21595..21694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21695..22999)
                     /locus_tag="PKV30_00765"
     CDS             complement(<21695..22999)
                     /locus_tag="PKV30_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRL28781.1"
                     /translation="MDLLLIVFLTLLNGLFAMSELAVASSRKARLAAMEESGDAGAGA
                     ALRLMERPTQFLSTVQVGITSIGMLNGIVGEAAFSGPVAEWLAGFGLSEKVASIGATA
                     LVVTAITFTTIIFGELVPKRIGQMFPETVARWIAQPMTWLATGAKPFVALLSLCTQAV
                     LKLLRIDESHGRAVTEEEISASLEEGVDAGLIEEHEHQMVRNVFHLDDRSLSSLMVPR
                     GDIEWLEAGQSVGTALDYVASQSENGGHSWYPVCDGGLDEVIGIISVAKLIKLGAASS
                     ESIESHVTPAAFVPETLSGMELLEQLRAKSGRMVFIVDEYGVVQGLMTPRDLLEAITG
                     ELKPGAHIDAWATPQPDGAWLLEGLMPINEMKARLDIDDLPHEDKGRYNTLAGLLLAE
                     AGRLPSIGDVIECAGWAFEVIEMERRRIDKVLARPVAAAEETG"
     gene            23036..23878
                     /locus_tag="PKV30_00770"
     CDS             23036..23878
                     /locus_tag="PKV30_00770"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HRL28782.1"
                     /translation="MALYMIGDVQGCDAALGDLLEKVAFSPSRDRLILLGDLVNRGPA
                     SLAVLRRMVALGDAAACLLGNHDLHLLAVSQGVRKPHRSDTVADILAAPDRDALLDWL
                     RHRPLALHEHGVLMVHAGVLPQWTVVQTLVLATEVEKVLQAAEWADFMHQMYGNQPDQ
                     WRDGLQGAERLRVIVNALTRLRYCTAQGRMEFDTKAGGLDGAPPGYMPWFDVPGRQTT
                     GDVVAFGHWSTLGWIDRPNVISLDTGCVWGGCLSALRLGAGARERELIQVRCPQAQAP
                     GGAA"
     gene            23917..>24145
                     /locus_tag="PKV30_00775"
     CDS             23917..>24145
                     /locus_tag="PKV30_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HRL28783.1"
                     /translation="MKRLALGLLLLMAALYVSATALEPRYAALGYIASFAEAGMVGAI
                     ADWFAVVALFRHPLGLPIPHTAIIPANKDRIG"
BASE COUNT         4406 a         7791 c         7762 g         4086 t
ORIGIN      
        1 accaggtgct gaacgccaag caacacgcgc gcgaggccga catcgtggcg caggccgggc
       61 ggccgaagat gatcaccatc gccaccaaca tggcgggccg tggcaccgac atcgtgctgg
      121 gcggcaacgt cgagaaggcg gtcgaggcgc tggaggccga cgaatcgctg gacgacacca
      181 cgcgccgggc acgcatcgac gcgctgcgcg cgcaatggca gaaggaccat gaggcggtca
      241 aggcgatggg cggcctgcgc atcatcgcca ccgagcggca cgaaagccgc cgcatcgaca
      301 accaactgcg cggccgctcg ggccgccagg gcgatccggg ttcgtcgcgc ttctatttga
      361 gcctggacga cgcgctgatg cgcatcttcg ccggcgaccg cgtgcgcgcc atcatggaca
      421 agctgaagat gcccgagggc gaagccatcg aggccggcat cgtcacccgc agcatcgaaa
      481 gcgcgcagcg caaggtcgag gcgcgcaact tcgacgtgcg caagcagctg ctcgaatacg
      541 acgacgtgtc gaatgaccaa cgcaaggtga tctaccagca gcgcaacgac attctggatg
      601 ccgaagaact ggcagctcag attgccggcc tgcgcgaagg ctgcttcatg gatctggtgc
      661 gccagtacgt gcccgccgaa tcggtcgagg agcagtggga cattccggcg ctggagaaag
      721 tgctggccga ggagtggcag atcgagctgc cgctgcaaaa gcagatcgag agcgcatcgg
      781 ccatcaccga cgacgacatc gtcgagaccg tggtcaaggc cgccaacgac gcctacgccg
      841 ccaagcttgc gctggtcggc cccgagaatt tccgccagtt cgagcgcgcc gtgctgctgc
      901 aggctatcga cacgcactgg cgcgagcacc tctcggcgct cgactacctg cgccagggca
      961 tccacctgcg tggctacgcg caaaagcagc ccaagcagga atacaagcgc gaggcgttcg
     1021 agctgtttgg ccagttgctc gacgtggtga aaaacgaagt cacccgcacg ctgatgaacg
     1081 tgcgcatcca gtcgggcgat caggtcgagc aagccgccga agagatggag cagcgcggcg
     1141 agcgcatcac caacgtgacc tacaccgcgc ccaccgaaac cggcgaagcc gagacgctgc
     1201 ctgaattgca ggtgcgcacc ggcgcgctgc tgggcggcgc ggccgcggcg gcggtgccgc
     1261 gcgtgggccg caacgacccg tgcccttgcg gcagcggcaa gaagtacaag cagtgccacg
     1321 gcgcgttgag ctgatgcgcg gccaccgcac gcacgacacg ggcttcggcc cgtgtttgct
     1381 ttgcgctgtg aatactattg atatgatagc tgtccgcgct tgttcatcaa gcgccagagg
     1441 ctgatttcgt tcaaaaccac cttcagcaag gaagcaccat gcccgtgaac ctgagcgccc
     1501 ccgtggcttc ccacctgcag gccgtgcccg gcgtgcgcat cggcgtcgcc gaggccggcg
     1561 tgcgcaaggc gggccgcaag gacttgacgg tgatgctgct ggatgcgggc accagcgtcg
     1621 gcggcgtgtt caccagcaac cgcttttgcg ccgcgccggt gcagatctgc cgcgagcgcc
     1681 tgcagggccg ccccgacatc cgcgcgctga tgatcaacac cggcaacgcc aacgccggca
     1741 cgggcgaaga cggcctggcg cgcgcgcggc agaccagcgc cgcgctggcc gcgctgctca
     1801 agatcgagcc ggcgcaggtg ctgccgtttt cgaccggcgt catcatggag ccgctgccgg
     1861 tcgagcgcat cgaagccggc ctgcccgccg ccattgccga cgccgcgccc gaccactggg
     1921 cgcgcgccgc cgaaggcatc atgaccaccg acacggtgcc caaggctttc agcgcccagg
     1981 ccaaggtggg cggcgccgag atcaccatta ccggcatcag caaaggcgcc ggcatgatcc
     2041 gccccaacat ggcgaccatg ctgggttttc tggctaccga tgcgcgcgtc gatcaatcgg
     2101 tgatgcacca gttggcgctg gagttggccg aaggctcgtt caaccgcgtc accatcgacg
     2161 gtgacacgtc gaccaacgat tcgttcatcg tcatcgccac caaccaggca gcgaatgcgc
     2221 cggtcacgtc gctcgattcg gccgacggcc aggccttgaa gcaggcgctg ctcggcgttg
     2281 cccgcctgtt ggcgcaggcc atcgtgcgcg acggcgaggg cgccaccaag ttcatcacgg
     2341 tgcaggtcga tggcggcaag accgcagccg aatgccgcca ggtggcctat gccatcgcgc
     2401 attcgccgct cgtcaagacc gcgttcttcg ccagcgaccc gaacctgggg cgcattctgg
     2461 cggccgtggg ttatgcaggc attgaagacc tggatcagac caggatcgac ctgcacctgg
     2521 atgatgtgca cgtggccgtc aacggcggcc gcaacccggc gtaccgcgaa gaagacggcc
     2581 agcgcgtgat gaagcaaagc gagatcaccg tgcgcgtcaa cctgggccgc ggcacggcca
     2641 gcgacacggt gtggacgtgc gacttgagcc acgattacgt gagcatcaac gccgactacc
     2701 gttcctgaga ttgctatttt taggatagct gcttgcgctt gatacacaag cgccagaggc
     2761 cgatatgact gaaaaccttg cccgcctgat cgacaaggcc gagcgcctga tggcgcgcat
     2821 cgagtcggtg ttgccgcagc ccttgtcggc gcccgactgg tcgcagtcgg tggcctggcg
     2881 ctaccgcaag cgcgccagcg gccacggcgc gctggagccg gtgcgccacg tcggcgcgat
     2941 ggcgctcagc gacctgaaag agatcgaacc ccagaaggaa aagatcgagc gcaacacgcg
     3001 ccagttcgtc gaaggccggc cggccaacaa cgtgctgctg acgggcgcgc gtggcaccgg
     3061 caaatcctcg ctcatcaagg cctgcctgaa cgcctatgcg ccccaggggc tgcggctgat
     3121 cgaggtggac aaggccgatt tgactgatct gcccgacatc gtggacgtgg tggccggcct
     3181 gccggagaag ttcatcgtct actgcgacga cctcagcttt gaagaaggcg aggcgggtta
     3241 caaggcgctc aaaagcatcc tcgacggcag cgtggccgcc agcacgccca atgtgctggt
     3301 ctacgccacc agcaaccgcc gccacctgct gcccgagtac atgaaggaga acctcagcta
     3361 cacgcacacc gacgatggcg aggtgcaccc gggtgaggtg gtggaagaga agatttcgct
     3421 gtccgagcgc ttcggcctgt gggtcagctt ctacccgttc agccaggacg aatacttggc
     3481 catcgtggcg cagtggctgg gcgcgctggg cgcgacgccg gcgcagatcg aatcgtcgcg
     3541 tgccgaatcg ctggtgtggg cgctggagcg cggttcgcgc tcgggccgcg tggcgtatca
     3601 gtttgcgcgc gacttcgtgg ggagaaatga cgccccctga gtcgcgctgc gcgcgccttc
     3661 ccccactggg gacgacgcca gcggccgggc caagcccgct ccgcggcacc ccagcgtggc
     3721 ctgctccgcg gcctttggtt gcttgaactg ttgtgggtga catgacggac gtgctgaaag
     3781 ccgatgccca catccagcgc caggcggtgg gcgagcggcc ggtggttgac gtcgccgtgg
     3841 gcgtgctgct gcaggcggac gggcgctttc tgctgacgtc gcgcccggcg ggcaaggtgt
     3901 atgccggcta ctgggagttt ccgggcggca agctggaggc gggtgagagc gttgcagagg
     3961 cgctggcgcg cgagctgcac gaggaactgg gcctggtcat cgcgccatcg gatgttcagg
     4021 cgtggcgcga gcaattggtc gactacccgc acgcgctggt gcggcttcat ttttgcaagg
     4081 ttcacgcgtg gcgtggtgat ttgcagatgc gcgaagggca gtcgtttaca tggagcggct
     4141 tgccggtgca ggtggcgccg gtgctgccgg gcgcgctgcc ggtgttggag tggctgcaag
     4201 cagagcgtct gctattggat aaatagctgc ttgcgcttgt ctggcaagcg ctggaggccc
     4261 aaaatcttct ggatccgggg cttactgcag acgtgtggct tcgctcggtt cggcgccatc
     4321 gtcttcttcc acctgcaccc gaaaactctc gttggcccag gcgcccaggt cgtgcaactt
     4381 gcagcgctca ctgcagaacg gccggaagtg gttgtcgggc gtgaacaggc tggggccgcc
     4441 gcaggctggg caagtcacgg tgcgcggctt cgctgaattt ggggctcggg cgggattgct
     4501 catgcgcaca aggccatctc gaaaggcgca tcctcgcgcg cgatgtgcag cttgccgtcg
     4561 gcgtcttgtc gcatcaggcg caccgacgcc atcaagcggt tgccgctgat ttcaggaatc
     4621 agttgcatgg attcgtcgat gcgcaggcgc agcaactgga aggtgcggcc ttgcggcagc
     4681 gtctgctgaa actgaccggc cgtggccatg accttctgcg gtttgccgga gtcgcgcaag
     4741 atctgcagca gcagcgtgat ggcctgggcc atcggggcca gcgacgcggc ccaagcctgc
     4801 agatcggcgc gccgcgcggc cggttggccc tgctgccatg cgtgataggc cggaagatcg
     4861 aaaccgcagg tgccgcccgg aatgactgca cggctgcgga tgctcatcaa ccattcgttg
     4921 tcggtcaact cgtggcccgg tttgccaagc tgctggttga gcttttgaaa ggcgtcgtcg
     4981 agttgcgtca ggatggcgtc cagcgaagcc tccgaaatgg ccgggttgcc acggtaggcg
     5041 ccgatgtggg ccttctgctt ttccaggtcc ttcagcactt ccgacttcag gtcggcgcgg
     5101 ctggtcacat ccatcacttc gaagatggta atgagggcga aatggtggtc caggggtgca
     5161 tcgcgcgcca gcaactggcc cagtcggccg aaaaggtgtt cgagcctgag gtaggtgcga
     5221 atgcgctcgt tgaaggggta ctcgtacagg atcacgcggg gcgccttttt gacgcccgca
     5281 tcatagcccg aaggtctgcg ccagccgggc ggcctgggcg cgcaacgcgt cctgggtgac
     5341 gcccgcgccg ttgaaaatca ccaggtcggc cgctgccaga cgctgcgcgc gcgatgcctg
     5401 tgcggccacg atttgttcga cctgatcgcg cggcagggcg ttgcgcgcca tcacccggtc
     5461 aatctgggtg gcgctgtcgc agtcgatcac cagcacgcga tcgaccttgg cgcgccaacg
     5521 cgcgccggat tcgaccagca gcggaacatc gaacaccagc aggcggcgac ctgcgtccag
     5581 cgccagtcgg gcctggcggt cggtctcttg ggccaccagc gggtggatga tggcttcgag
     5641 ccgctggcgc gcgtcggcgt catcgaacac cagcgcgcgc atcgcggtgc gatccagcgc
     5701 accgtctggg gcgatcaaat gggcgccgaa ctgtcgcgcg atgctgggca gggctgctcc
     5761 gcccgctgcc gtggtggcgc gtgaaatcgc gtcggcgtcg atgacggcgg cgccatgcgc
     5821 tgccagcatc tgggccacgg tgctcttgcc gctgccgatg ccgcccgtga ggccgaggag
     5881 cgtggccgcg cccatgcccg cttggcttac agacccacca cgcgcaggat cgactgcggc
     5941 ccgaagatca tggcggtcaa tcccgccatc gacaggaagg gcccgaaggg cactacgccg
     6001 ccttcgcgca gcgaactcat cagcttcatg ccaatgccga cgagggcgcc gatgaccgat
     6061 gccatcagga tgatgggcac cagcgcctgc cagccaaacc acgcgcccag cgcggcgaac
     6121 agcttgaagt cgccaaagcc catgccttcc ttcccggtgg ccagcttgaa tccccagtag
     6181 accagccaca gcgagagata gcccgccgcc gcgccccaca ccgcgtcacc caggcgcacg
     6241 cccgtgatgc ccgacgccgc ggcgagcaag cccagccaaa gcagcggcaa ggtcaggtcg
     6301 tcgggcagca gcgtggtgtc ccaatcgatc aaggtcagcg ccaccagcac ggcggcaaac
     6361 acgcaccagg cggcggcggt caaggtccag ccccagtgcc agacgcacca ggcaaacagc
     6421 aggcccgtga ccaactcgac caacggatag cgtggactga tcggcgtcgc gcaggccgaa
     6481 cacttgcccc gcagaaacag gtagctcagc acggggacgt tctcgtacca gcgaatctgg
     6541 tgaccgcatt tctggcagcg cgatcgcggt accagcagat tgaatttttc ttgcgtgggc
     6601 ggtgcttgac cggtcatctc cgcgcactcg gcggcccatt gccgctccat catcttgggc
     6661 aggcgataga tgacgacgtt caggaagctg ccaatgagca gcccgaaaac accgcccagt
     6721 gcgacctcca gccaaagccc ctctggcatc agacgacctg gccaagcttg aagatcggga
     6781 ggtacatgga caccacgatg ccaccgatga tggtacccaa aatgacgatg atgatgggtt
     6841 ccatcaggct tgaaaggccg gcaaccatct catccacctc tgcctcatag aaatcagccg
     6901 ccttgcccag catgtgatcg agcgcgcccg actcctcgcc aatggccgtc atctggatga
     6961 ccatgctcgg aaagatgttc gcgttggtca ttgctgccgt cagactggta cccgcggaga
     7021 cttcctgctg aatcttgtcg gtggctattt tgtagaccga gttgccggcg gccccgccga
     7081 ccgaatcaag cgattcgacc aagggaacac ccgctgcgaa catcgtggac aacgtccgcg
     7141 tccagcgtgc aaccacagat ttttcgatca aatctccaaa gatcggaacc cttaacaaca
     7201 gccgatccat gaacatctga accttctcgt tccgcttcca cgcttgcatg aagaagtaga
     7261 tgccgaagcc gaggcctcca aaaataagcc accaccattg gacgaagaac tcgctcatgg
     7321 cgatgacgaa cagggtcggc gctggtagat cggcaccaaa gttagaaaac acttccttga
     7381 aagcagggat cacgaaaatc atgatcaccg aaacaaccac aaaggcgacc acgatcaccg
     7441 caatgggata catgagtgcg gacttgatct tcgacttgat ggcctccgtt ttctccatgt
     7501 aggtggccag tcgatccagc aggctttcca agatgcccgc cgcttcgccg gcttcaacca
     7561 ggttgcaata aagactgttg aaatacagcg ggtacttgcg gaaagctgca gagagcgaag
     7621 tgcctgtctc gatatccgcg cgaatgtcgt ttaacaactt cgtgacgctc gggttcggat
     7681 ttcctcgtgc gacgatgtcg aacgactgca gcaagggcac gccggccttc atcatcgtgg
     7741 ccaactggcg cgtgaagatc gcgatgtctt tcggtttgat cttcttgccc gcccgcatgc
     7801 ggcgcttctt tatcttggtg gcaaagatgc cctggcgtct cagtgtcgcc tgcacctgat
     7861 tttcaccgcc agcgcgggtt tcgccccgaa cgatcttgcc gttgcgatcc ttgccttccc
     7921 attcgaagac gaactctttg acgcccttgg cgccggcgac ttgggttgcc atgtgttgct
     7981 cgacctctgg ttttctacgg taggcgaacg cttactcgtt cgtgcaagcc agcacttcct
     8041 caagtgaagt cacgcccagc ttgaccttgt gcaggcccga ttgccgcagt gagcgcacat
     8101 cttcgcgccg cgcctgctct gcgatttcca gcgcactacc gtcggcgagg atgatgcgtt
     8161 gaagctcctc gctgatgggc atgacttcat agataccaac gcgcccctta tagccgttgt
     8221 tgcacgccga gcaaccgact ggcttgtagg gcgtccagct accgtcaagc tccagttcgc
     8281 tgaaaccagc ctccatcaat gtttcgcgcg ggatatcgac cggcgccttg cagtgggcgc
     8341 acaaccgccg ggcgagacgc tgagctgtaa tcaagatcac gcttgacgcg atattgaacg
     8401 gcgcaatccc catgttccgc atacgtgtca acgtcgtcgg tgcatcattc gtatgcagcg
     8461 tcgacaacac caagtgcccg gtctgcgcgg ccttgatgga gatgtcggca gtttcaaggt
     8521 cacggatctc gccgaccatg atgatgtccg gatcctggcg cagaaacgcc ttgagggcga
     8581 cggcgaaggt caggcccgcc ttctcgttga cgttgacctg attgacgcct ggcaagttga
     8641 tttcggatgg atcttcggcc gtagcgatgt taacgcccgg cttgttgagc aagttcaggc
     8701 aggtgtagag cgataccgtc ttgcccgaac ccgtggggcc ggtcaccagg atcatcccat
     8761 agggccgctc gatcgctttc agcagtcgct ctttctcgat cggttcgtaa cccagcgcgt
     8821 cgatacccaa cttggcactg ctcggatcca gaatacggat cacgattttt tcgccgaaca
     8881 acgtgggcag cgtgctgacg cggaaatcga tgacccgatc aggcccgatc ttcagcttca
     8941 ttcgtccgtc ctgtggcacc cgtttctccg agatatccag acgcgacagt accttgatgc
     9001 gcgacgccaa cttctccttg atggcgacag gcggtgacac gatttcccgc agttccccat
     9061 cgacgcgata acgcacgcgg tagttgtgct cgtacggttc gaagtgcaaa tccgatgcgc
     9121 gcatgttgaa agcatcgacc agcatcttgt gcagaaactt gacgactggc gcgtcttcga
     9181 catcggtctg aggtgcttcg gtctcctccg ctgcgccatc ggttcccaaa gaggcttcat
     9241 cgaactcgaa atcgccgccg acgatactgt ccatcgcctg cgtggctgtt ttcgtcgttt
     9301 cctcgatcag acgcgacaac ttgtcgaact cgccaatgac ccattcgacg cccatctgcg
     9361 tcgcaaattt gaccttctcg gccgcctcat gatcagacgg atcggcggtg acaacgatta
     9421 atcgtccgcc gcgcttgctc agcgcgagga tgcggtaggt ggcagacagc ttgggatcaa
     9481 gcaaatcgcc gggcaggcgc tggacatcga gcgcatcgac atctagcagc ggcgccgaga
     9541 aagcctgcga catcgtgtgc gccaagtccg aagcggaaat ggcccccgat tcggtcaact
     9601 cggttatgaa attacgatta gccgccttgg ctttgcggaa cacatcatcc gccgtctgtt
     9661 ggctgacctt gcccgccatg accagtgcgc gccccacacc gggcagcacg gcgggtgcag
     9721 cagctttgga aatcggatca gcaacagcca tagggagcat cgtcaagcag gcaataaagc
     9781 cagttgatca tcgctgatca gtgaaaatct gtaaacgatg tcgctgtgga atgtgtgcag
     9841 caatcgacac atcaagcgct gagggaggca aacggcccaa tccgcgcggg aatggtcggg
     9901 gtgagaggat tcgaacctcc ggcctctacg tcccgaacgt agcgctctac caggctaagc
     9961 tacaccccga tggtgtcggc gcacgttgag ttggtcacgc gcgccgttga agcaactgag
    10021 ccgccaattc tagcaaactg tcggtgtagt gattggccgg cagcgcgcgt gcggccgcga
    10081 tggcgcgttc ggcttgcgcg gcggcggcgg cgcgggtgcc atccaacgcg cccgttcggc
    10141 gcacgatgtc tacgacgcgc gacaactcgt cggtgcttcc gctttcaatg gattgacgaa
    10201 cgatgcgtgc atcagcgtcg ttgcccagtt tcatggccag gataagaggc aaagtggcct
    10261 tgccttcacg caggtcatcg cccaggttct tgcccatctc cgccgcatcg ccgtcgtagt
    10321 cgagcacatc gtcgatgacc tgaaacgcgg tgcccagcga ctgtccgtac tcggcgcagg
    10381 cggcgcgtgt cacgggcgag ctgccagcca gaatcgctgc cagctcgcaa ctggcctcaa
    10441 acaacttggc ggttttggag cgaatcacgt gcagatatgc gttttcgtcg agcgacgcgt
    10501 cgtgcatgtt catcagttgc agcacttcgc cttcggcaat gatgttggtc gcattggcca
    10561 gcacctgcat gatctgcatg ttctggctct cgaccatcat ctggaaggcg cgcgaataga
    10621 gaaagtcgcc aaccagcacg ctcgccgggt tgccaaacac ctcgtttgcc gtggcgcggc
    10681 cgcgccgcag cgtcgattcg tccaccacgt cgtcgtgcag cagggtcgcg gtgtggatga
    10741 attccaccac ggcggccaga ttgaagcgct gcgcatcacg gcagcccagc gcgccgcaca
    10801 tgagcagcag cagcacgggc cgcagccgct tgccacccgc cgagatgatg tagctggcca
    10861 cctgaccgac cagcggcacg cccgattcga gccggcgcaa gatggtggcg tccacttcct
    10921 gcatgtcggc cgcggccagc gccagcgcgc ggtggatggg ttgggggtcg gagggcactg
    10981 cagcaggtgt gtgaaacttc tggcgtggcg cgccctggcg ccccacgaac cggcgggatt
    11041 ataggtttgc acgctattga gccgatgcgt gtctgcctgc ccggcgcaca tgtctatgca
    11101 aatgactgaa tcgccactat aatgcgcggt tctgtcagaa attcgtctgg cagatccatc
    11161 ctaagaggat tcctatgtac gcggtcataa aaaccggtgg caagcagtat cgcgtggctg
    11221 ccggcgaaaa aattaaagta gaacagattg ctgcggacgt tggccaggag atcacgttcg
    11281 atcaagtgtt ggctgtcggc aacggcggcg acctgcagat cggcgcgccc ctggtgtccg
    11341 gcgcttccgt ggtggccaag gtggtcgccc atggcaagca cgacaaggtg cgcatcttca
    11401 agatgcgtcg tcgcaagcac tatcaaaagc gccagggcca tcgccagggc tataccgaac
    11461 tcgagatcgg caccatcagc ggcgccggca agtccaagga gtaaagcacc atggcacaga
    11521 aaaaaggcgg cggctcgacg cgaaacggcc gcgattccaa gcccaagatg ctgggcgtga
    11581 aggtgtttgg cggccaaacc atcactgcgg gcgccatcat tgtgcgtcag cgcggcacgc
    11641 gtttccacgc gggcgccaac gtgggcgtgg gcaaagacca caccctgttc gccttggtcg
    11701 acggccaggt gtcgtttgcc accaagggcg cgctgaacaa gcaagtcgtg ctggtgacgc
    11761 cggcagcctg acttcggtcg cagttgtcag ccggagcccc gcccgcgcgg ggctttgttg
    11821 tttctggcgc accgtaaact gtacgaacca tgaaattcgt tgatgaagcc tttatcgaca
    11881 tcgccgccgg cgatgggggc aacggctgtg tctcgtttcg ccacgagaag tacaaggaat
    11941 tcggcggccc caatggcggc gacggtggtc ggggcggcca cgtgtttgcg gtggccgacc
    12001 ccaacctcaa cacgctggtc gacttccgct actcgcgccg ccacgaggcc aagcgcggcc
    12061 agcacggcat gggctcggac atgttcggcg ccgcgggcga cgacatcacg ctgaagatgc
    12121 ccgtcggcac cctgatcaat gacgccgaaa ccggcgaaac gctgtacgaa ctgctgacgc
    12181 ccggcgagaa gatcatgatc gccaagggcg gcgacggcgg ctttggcaac atgcgtttca
    12241 agagcgccat caaccgcgcg ccgcggcaga aaacgccagg ttggccgggc gagaaaaaaa
    12301 gcctgaagct cgaactgaag gtgctggccg acgtcggctt gctgggcatg cccaacgcgg
    12361 gcaagtccac gttcattgcg gcggtgagca acgcgcgtcc gaagatcgcc gactacccct
    12421 ttaccacctt gcaccccaac ctgggcgtgg tgcgcgtggg gccggagcaa agcttcgtgg
    12481 tggccgacat tcccggcctg atcgagggcg cgagcgaagg cgcgggcttg ggccatcagt
    12541 ttttgcgcca tttgcaacgc acgcgcctgc tgctgcacat ggtcgacatt gcgccgtttg
    12601 acgagggcgt cgaccccgtc gcgcaagcca aggccatcgt ggcagagttg aagaaatacg
    12661 acaaggcgct gtacgacaag ccgcgctggc tggtgctcaa caagctggac atggtgccgg
    12721 tggaagagcg cgccgcgcgg gtgaaggact tcgtcaagcg cttcaagtgg aagggcccgg
    12781 tgttcgagat ttccgctctg acgcgcgaag gctgcgagcc cttggtgcag gcgatttatc
    12841 gccacatccg caccgagcag gatgctgccg cgcccgaggc cgcgcccgat ccgcgctttg
    12901 cgcctgaaca agaatagcta cataaaaaag agctgctcgc gcttgttgta caagcgctgg
    12961 agccttaaat catggacaac agcacatccg aagtgttgcg ccaggcgcgg cgcattgtga
    13021 tcaaggtcgg ctccagcctg gtgaccaacg aggggcgtgg gctggacgaa gcggccatcg
    13081 gcgaatggag ccgccagatg gcgctgctgg ccggcgagcc cggcgcgcgg cgcgaagtgg
    13141 tcatggtctc cagcggcgcc atcgccgagg gcatgaagcg cttgggctgg cccaagcgcc
    13201 cgcgcgaggt ggctgagctg caggccgccg ccgccgtcgg ccagatgggc ctggcgcaga
    13261 tgtacgagac caagctgcgc gagcagggcc tgcgcagcgc gcaggtgctg ctcacgcatg
    13321 ccgacttggc cgaccgcgag cgctacctca acgcgcgctc cacgctgctg acgctgctgc
    13381 aataccaggt gctaccggtc atcaacgaga acgacaccgt ggtcaccgac gaaatcaagg
    13441 tcggcgacaa cgacacgctg ggcgcgctgg tggcgaattt gatcgaggcc gacctgctca
    13501 tcatcctgac cgaccagaag ggcttgtaca cggccgaccc gcgcagcgac ccggcggccg
    13561 agttcgtgca cgaggcgcgc tctggcgacc tggcgctgga gcgcatggcc ggcggcgccg
    13621 gctccagcat cggcaagggc ggcatgctga ccaagattct ggccgccaag cgcgcggcgg
    13681 cgtcgggcgc ctccacggtg attgcctggg ggcgcgagcc ggatgcgctg ctgcgcctgt
    13741 cgcgtggtga gcgcataggc acgctgctgg tggcgccgac gcaaaagctg caggcgcgca
    13801 agcagtggat cgccgaccac ctgcaattgc gcggcgcgct ggtggtggac gaaggcgccg
    13861 tggccaagct gcgcggcgcc ggcaccagct tgctgccgat cggcatgacg cgggtcgaag
    13921 gcgtgtttgc ccgcggcgac gtggtggcga tccgcgatgc cgccggcgcc gaggtggcgc
    13981 gcgggctggc caactattca agcgccgagg cgcgcttgct gtgccgcaag ccgtcgagcg
    14041 aaatcaacgc gctgctgggc tacatggctg aatcggagat ggtgcaccgc gacaacatgg
    14101 tgctgtcgcg tcaatagcca aatcggccgt tggcgcttaa tgggcaagcg cgagcagcta
    14161 tcaatagtag agcgtcttgt tgcccgactt gagttcgcgc accatgtcgg ccacggtctg
    14221 gcgcacgccc gtgccgatgc agatgccgtc gcgcgcgagc cggaacgggt gctgcaccat
    14281 gatgctggtt tgcccgcgca tgcttttcca gtcgttctcg ccgctgggcg accacgggtt
    14341 gccttgcacc tggcgcgcat agacccggta ctcacccgtc gcgcagcgaa tgccttcata
    14401 actggcattg accgccgatg gcccgcgtgc caccaccaca tagcgcacga tgccggtctc
    14461 gtggttgatg cttatggttt cggggtcgat gcccatgcgc acggacgacg agcgcggcat
    14521 ctcgatgtcg atcagcttgc tggtgctgta ggccggcggc ggcggcaccg tgtcttcgcg
    14581 ccagtcggcg cggtcgaggt cggcgtactg cgccatggcc ggcagcgtca gcgctgccaa
    14641 ggcaatggcg accgcgcgcc atgcgtgcgc caacagccgc ctcatgattc gtccctcggg
    14701 ccgaacggcg ggcacggcac gttttcgcgc gttgcgcccg ggggcagcgc catcgagggc
    14761 gggccgacgc ggcggtcatc gcgatcctgc gtctggatgg gcgacaactc gtcgtgctcg
    14821 cgcggacgca tgccgccgcg caggtagcgg ttgcggtgca cttgccgggg cagaaagcgc
    14881 gccagctcgg tcagcgccat ctcgtacacg ccgcgcttga actcgaccac cacgtccagc
    14941 ggcacccagt agtcgttcca gcgccaggcg tcgaactcgg ggtggttggt ggcgcgcaga
    15001 ttgagttgcc agtcgtgcgc catcaattgc agcagatacc agatctgctt ctggcccttg
    15061 tagtgaccgc gcgcgtcgcg gcggatgaac cggtctggca cctcatagcg caaccagtcg
    15121 cgggttcggg ccacgatgcg cacatgctcc ggctgaaggc cgacttcctc gtgcagttcg
    15181 cggaacatgg cctgctcggg cgattcgccc cgatcaatgc cgccttgcgg aaactgccag
    15241 gaatgggtgc ggatgcgctt gccccaaaat acctgattct tctggttgag caagatgatg
    15301 ccgacgttcg ggcgaaagcc ttcccggtcg agcataatca aaccccaatt tcaatagttg
    15361 tttccattat gcacggcgcc ccatggccgg caagtgggca caaaccccat ccgcggtgtg
    15421 gaatccatca cgccgctgcc tgcagatgaa agcctctcag ttcctgattt ccacgctcaa
    15481 ggaggcgccg gccgacgccg aagtgctgag ccatcagctc atgacccgcg ccggcatgat
    15541 caagaagctc ggcgccggca tttacaccta catgcccatg ggcctgcgcg tgatccgcaa
    15601 ggtcgaggcc atcgtgcgcg aggagatgaa ccgcgccggc gccatcgaat gcgtgatgcc
    15661 ggtgatccag ccgggcgagc tgtggaccga gagcggccgc tttcaaacca tgggccccga
    15721 gctgctgcgc gtgcgggatc ggcacgagcg cgatttcgtc gtgcagccga ccagcgaaga
    15781 agccgtgacc gacatcgcgc ggcaggacct gcgcagctac aagcagctgc ccaagaacct
    15841 gtaccagatc cagaccaagt tccgcgacga gcgccgccca cgctttggtc tgatgcgcgg
    15901 gcgcgaattc atcatgaagg acgcctacag cttcgaccgc gacgaggcca gcgccaagct
    15961 gagctaccag aagatggccg ccgcctaccg cgccatcttc gaccgctttg gcctgaccta
    16021 ccgcgccgtg gcggccgaca gcggcgccat cggcggcgac ttgagcgagg agttccaggt
    16081 catcgcggcg acgggcgaag acgcgatcgt ctacagcact ggcagcgact acgccgccaa
    16141 catggaaaag gccgaggcgc tggcgcccgc tggcgcgcgt cccgccccgg ggcagccgat
    16201 gacgaaaaca cccacgccgg gcaagagcac ctgcgctgac gtggccgagt tgcttgcgct
    16261 gccgttgacg cggaccgtga agtcgctggt gctggccacc gaggaattga acgacgcggg
    16321 cgaagtggcc aaggtgcaga tctggctgct gctgctgcgc ggcgaccacg acatgaacga
    16381 agtcaaggtc ggcaaggtgt cggggctgga ccaaggcttt cgctttgcca gcgtgcccga
    16441 aatcatcgac cactttggca ccaagcccgg ctacctgggc cccatcggct tgaagaaacc
    16501 cgtgaagatc gttgccgacc gcgaggtggc ggcgatggcc gactgggtct gcggggcgaa
    16561 cgaggaagac tttcacatca ccggcgtcaa ctggggccgt gacctgcccg agcccgacct
    16621 ggtggccgac atccgcaacg tggtggccgg cgatgcggcg ccggacggcc ggggccagct
    16681 tgccatcgag cgcggcatcg agatcggcca catcttctac ctgggcacca agtacagccg
    16741 cgccatggac gcgacctttc tcgacgatga cggcaagccc aagcccttcg agatgggctg
    16801 ctacggcatc ggcatcacgc gcctgccggc ggccgccatc gagcagaacc acgacgagcg
    16861 cggcatcatc tggcccgacg cgattgcgcc gttcacggtg gtgatctgcc ccgtcggcat
    16921 ggatcgcagc gaaggagtca aagccgcagc cgaaggcctg tacgccgagc tgatgcaagc
    16981 cggcgtcgac gtcatcctgg acgaccgcgg cgaacgcccc ggtgccatgt ttgccgactg
    17041 ggagctgatc ggcgtgccgc accgcgtgac gattggcgac aagggcttga aggacggcca
    17101 gctcgaatac cagcaccgcc gcgacacggc cgcgaccaag gtgccggcgg gcgagatcgc
    17161 ggccttcgtg aaaggccgat tggcggcatg acggggcccg ccgcgcggcc tggccgcgac
    17221 gccttctctg agagaagaaa atgcctccag cgcttgttgg tattgcccgg ttcgctcctt
    17281 tttcatgagc aatcaagcgc cggcggtcaa ctggaagagc cgctggccga ttcggtgcgc
    17341 agcgccttga gttcggccat tgccaacagc gcgccgccgg tgccgacttt tgccaccacc
    17401 gaggcgcgcc tgtcctacct gcgctggctc tccagcatga gcgaccggct ctacagccgc
    17461 gtgaaggact tccccacgcg catcgagctg ctgcaaacca tctggtacga aagccgccgc
    17521 gcggggctgg acacgtcgat ggtgctgggc ttgatccagg tcgagagcgc gttccgcaag
    17581 ttcgccgtct ccagcgtcgg cgcgcgtggc tacatgcaga tcatgccgtt ctggacgcgc
    17641 gtgatcggcg atggcgaccc gggcaagctg tttcacatgc agaccaacct gcgctttggc
    17701 tgcgtgatcc tgcggcatta cctggaccgc gagcgcggcg atttgttcat ggcgctgggc
    17761 cgctacaacg gctcacgcgg caagccgccg tatcccaacg cagtgttcgc ggcgcaaaaa
    17821 aaatgggttt ttgccggcta gcgcttgtct ggcaagcgct ggcagctatc aaaatcgtag
    17881 ttattcagcg ctgggcggct cggcgcgcgt taccagcacc tggtcgatgc ggtagttgtc
    17941 gacgtccatc acctcgaagc ggtgctcgtt caccgtcacg ctgtcggtgc ggcgcggcac
    18001 gcggcgcagc atcatcatca gaaagccggc cagcgtgtcg tattcgccct ggccgggcag
    18061 gtcgtccagg cccagtgcgc gctgcacgtc ctgcatcggc gtgtggccgt cgatcagcca
    18121 gctgccgtcg ccccgccgca cgatcagatc ctcgtcctcg ggcgtgacca actcgcccat
    18181 caccgtgctc atcacgtcgt tgagcgtgac cacgccgacc acggtgctgt actcgttgac
    18241 gatgagtgcg aagtcctcgt gttgctcccg aaattgaagc agtacctcgg acaggctcag
    18301 cgaatcgggc accaccacca gcttgtgcag cagcccttcg tcggtcaggc ggatcggcgt
    18361 gcccttgagc acgcgctgga acaaatcctt ggcgtccacg tagcccagca actggtccag
    18421 cccgccatcg cacaccgggt aggtcgaaaa cggctcggcg gcgatcagca ggcgaatctc
    18481 ctcgtcggtg gcatcgcggc gcagccaggc aatgcggtcg cgcggcgtca tggcgctggt
    18541 gacggtgcgc gtgtccagct cgaacacgtt ctcgatcacc tgctgctcgg gccgatcgag
    18601 cacgccggcc tgcgtgccgg cttcggccag cgccaggatg tcgtccggcg tgatgcggtc
    18661 atcgcgctgc gtgggcagcc ccagcaggcg catgatgacg tcggtgcagc gcgtgtacaa
    18721 ccagacgatg ggtttgaaga cgagcagaaa gcgctccatc ggcccgacca ccgccatcgc
    18781 cacgcgctcg ggctcggcca tgcccaggcg cttgggcacc aggtcggcaa agaccacgaa
    18841 cagcgaggtc accgccgcca ccgaaccggc aaacgccagc gtggccgccg tgcgctctgg
    18901 caaccagttt ttcagcgcgg cggccaggta ggggctgagc aggccttcgc cgacgatgcc
    18961 gcccagaatg gcgatggcat tcaagccgat ctgcaccgcg gtgaaataaa agcccggctg
    19021 ctcctgaacc cgcagcacgc gcagcgcgcg cgcgtcgcca tcgtcggcca gctggcgcag
    19081 gcgcaggcgg cgcgaggcgg ccagcgagat ctcggccacg gaaaagaacg cgctggccgc
    19141 gatcagcagc acgatgatca gcaggctctg ggtgaagctc attttgggga agggcggcgg
    19201 cgctgatggg cgggcgagag tgcccggccg gtgcgcgagg gcagtgtaca cagccgcgtg
    19261 ggcacccgat tcaggtgcca tcacgtagga gatggccgac atgcgcgggt catgccgctg
    19321 cctataattt ttggttcgag gggcgccgcg cgtgatgcgc atttcttttc atggtggcgt
    19381 gccgtttctt gatgacaaga accagaaagg tgcacattca tgtttgacgt actgatccgc
    19441 gaggccgcca cccgcttcgg cctgggcgac aaagccctgc cggtggccca gatgctgctg
    19501 gcctacatga ccaaccggga cacgggcggc ctggcgggat ttctggagaa gttcaaggcc
    19561 gccaacctcg ggcccatcgt gcaatcgtgg ctcggcggcg gccccagcgc ccaacccatc
    19621 agcaacagcc aggtcgagac cgtgctgggc ggcacgggcg gcctgctgtc gctgatcacc
    19681 tcgcgcctgg cggtgaaccg cgacaacgtg accgccgcca ttggatatct cctgccggcc
    19741 atcgtcggtc gcttgacgcc cggcggcagc gtaccggcca ccgtgccgcc tgaggtggcg
    19801 agcctggcgg ccgccggcca aagcctgctg gcagcccctt cgacggcagc ggccgccgcg
    19861 ccggccgcgg gcggcggcat gatgaagtgg ctgccctggg tcatcgtggc cgcggcagcg
    19921 ctgatcgggc tgtcctattg cggcaagaag acgcccgcgc cggacgccac ggtgccgccc
    19981 gcagcttccg cgccggcaga acccgcggcc gtgccgccgc ccgtgacgcc agccagcgag
    20041 ccgcccaagg ccagttttgg cggtggtgcg ccgatggcac cggcgtccgc gcctgctgag
    20101 ccggcctcca cggtcgcagc gccggccgac gagcccgccg gttccgccgt ggtcagcctg
    20161 atcgacgcca acaccatgcc cgcgctgaag gtgtacttcg attctggcaa gaccgacgtg
    20221 cgcgccgact ttgccgcgaa gtcggccgat ctggtggctt acatgaaggc caacccggac
    20281 gccaaagccg tcatctccgg cttcaacgac ccgaccggca acgccgcggc caacgccgag
    20341 ctgtcgaaga accgcgctca agctgtgcaa gccgcgctgg tagcggccgg cgtggcgcaa
    20401 gaccgcaccg tgctggagaa gccggctgaa accaccggta ctgccgccag caacgctgct
    20461 tcgcgccggg tcgaggttgt gctgcgcaag taatcaaaaa ggggctgtgc cgcgcgctcg
    20521 aacgagcagc ggcaccacaa aaaaacggac gcctcagcgt ccgtttttca ttgaatgaag
    20581 gggccgatca ggcgttgccc gtcaccagct gctgcaactc gcccgactcg tacatctcca
    20641 tcatgatgtc cgagccgccc acgaattcac ccttcacgta caactgcgga atcgtcggcc
    20701 actggctgta ttccttgatg ccctggcgca ccgcctcgtc ctccagcacg ttcacggtgg
    20761 tgagggccgc cggctcgacg ccggaggcct tcaggatttg aatggcgcgg cccgaaaagc
    20821 cgcacatcgg aaaactggcg ttgcccttca tgaacagcac gatgtcgttc tgtttcacga
    20881 ggtcgtcaat gcgctgctgc gtgttctggt ccatggtcga ttccttccaa acaatgcggc
    20941 ggattattcc accaacccag tgccattgtc ggctcggggt tgaccggcaa gcgccacggc
    21001 atgacacggc gcgagcgcat aggcctcgcg ctcgaaccgg ttgtcgagat acgcgtgccc
    21061 gccgcgccac cgttgccagg cgctgctcgc cagatacgcc ggcacgaaca acgcgcccca
    21121 gcgctcgtac tggcgcacgt gcacgtgctc atgggcgcgc accgcctcca gacacgtacc
    21181 gtccatcgcc agaatcacgt ggccgatggt gatggcatca aagcgcatgg gcgccggcag
    21241 ccggcggcaa ccgcgggtca gcaaaccgcc ttgaacttcg agcacgccgt gcacgaaggg
    21301 ccagcgcgcg cccagcgcgg ccatggtcag ggcagccacc aagccggcaa cggtccaggg
    21361 cagcggccaa aggtatttac aaaccatgcg catcacggcc actttgaaag ccggttgcgc
    21421 cgccaggttc ccgaaaaaca aaaaggcgcc tgagcggcgc ctttgcgata gccgaaatac
    21481 cgaagatcag gcgatcaggt atttgctgcg atccttgccg tcaatccact taggggcttt
    21541 gccgcggcct gtccacgttt gaccggtcgc gggatcgcgg tatttggggg ccacnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccggt ttcctcggca gcggcgacgg
    21721 gacgggccaa aaccttgtcg atgcggcggc gttccatttc aatgacttca aatgcccagc
    21781 ccgcgcattc gatgacgtcg ccaatgctcg gcaatctgcc agcctccgcc aacagaaggc
    21841 cggccagcgt gttgtagcgc cccttgtcct catgcggcaa atcgtcaata tcgagtcggg
    21901 ctttcatttc gttgatcggc atcagccctt ccagcagcca ggccccatcc ggttgaggcg
    21961 tggcccaggc atcgatatgg gcgcccggct tcaattcgcc cgtaatggcc tccagcaggt
    22021 cgcgaggcgt catcaacccc tgcaccacgc catattcatc gacgatgaac accatgcgcc
    22081 ccgatttggc gcgcaattgc tccagcagct ccatgccgct cagggtttcc gggacaaacg
    22141 ccgctggcgt gacgtgcgat tcaatcgatt ccgacgaagc ggcacccaat ttaatcagct
    22201 tggcgaccga gataatgcca atcacctcgt ccaggccgcc atcacacacc ggataccacg
    22261 agtggccacc gttttcgctc tggctcgcca cataatccag cgccgtgccc acgctttggc
    22321 ccgcctccag ccattcgata tcgccacgcg gcaccatcag cgatgacagc gagcggtcgt
    22381 ccaggtgaaa cacgtttcgc accatctggt gctcgtgttc ctcgatcagg ccggcgtcca
    22441 cgccttcttc caggctggcg gagatttcct cctccgtcac cgcgcggccg tgcgattcgt
    22501 cgatgcgcag cagcttcagc accgcctgag tgcacagcga cagcagggcg acaaaaggtt
    22561 tggccccggt ggccagccag gtcatgggct gggcaatcca gcgcgccacg gtttccggaa
    22621 acatctggcc gatgcgcttg ggcaccagtt cgccaaagat gatggtggtg aaggtgatgg
    22681 ctgtcaccac cagcgcagtg gcgccaatgg atgccacctt ttcgctcagg ccaaaccctg
    22741 cgagccactc ggccaccggc ccgctgaacg ccgcctcgcc cacgatgccg ttcagcatgc
    22801 cgatcgaggt gatgcccacc tgcacggtgg agaggaactg cgtcggccgc tccatcagcc
    22861 tgagcgccgc gccggcgcca gcgtcgccgc tttcttccat cgccgccagg cgcgctttgc
    22921 ggctggacgc caccgccagt tccgacatcg cgaacaagcc gttgagcagg gtaagaaaga
    22981 cgatcagcag caggtccatg caagccgggc gggcgcccgg tggaaaatga gcagcatggc
    23041 actctacatg atcggcgacg tgcagggctg cgacgcggcg ctgggcgatt tgctggaaaa
    23101 agtcgcgttt tcgcccagcc gcgaccgcct gatattgctg ggcgatctgg tcaaccgcgg
    23161 ccccgcatcg ctggccgtgc tgcggcgcat ggtggcgctg ggcgacgcgg cggcctgcct
    23221 gctgggcaac cacgacctgc acctgctggc cgtgtcgcag ggcgtgcgca agccgcaccg
    23281 cagcgacacc gtggccgaca tcctggccgc gcccgaccgc gatgccttgc ttgactggct
    23341 gcgccaccgc ccgctggcgc tgcacgaaca cggcgtgctg atggtgcatg ccggcgtgct
    23401 gccgcagtgg accgtcgtgc agacgctggt tctggccacc gaggttgaaa aggtgttgca
    23461 ggccgccgaa tgggccgatt tcatgcacca gatgtacggc aaccagcccg accaatggcg
    23521 cgatgggttg cagggcgccg agcgcctgcg cgtgatcgtc aacgcactga cgcggctgcg
    23581 ctactgcacg gcccaaggcc gcatggaatt cgacaccaag gcgggcggcc tcgacggcgc
    23641 gccgcccggc tacatgccgt ggttcgacgt gccgggccgc caaacgacgg gcgatgtggt
    23701 cgccttcggc cactggtcga cgctcggctg gatcgaccgg cccaacgtga tctcgctcga
    23761 caccggctgc gtctggggcg gttgcttgag cgccctgcgc ctgggcgccg gtgcgcgcga
    23821 gcgcgaactg atccaggtgc gctgcccgca ggcgcaagcc cctgggggcg cggcttgagc
    23881 acggcgccgc gcatccgtgt acgcgacgcc gccggcatga agcgcctggc gctcggcctg
    23941 ctgctgctga tggcggcgct gtacgtctcg gcgacggcac tggagccgcg ctacgccgcc
    24001 ttgggctaca tcgcctcgtt tgccgaggcc ggcatggtcg gcgccatcgc ggactggttt
    24061 gccgtggtgg cgctgtttcg tcacccgctc gggctgccga ttccgcacac cgccatcatc
    24121 ccggccaaca aggaccgcat cggcg
//
LOCUS       DAOMVO010000006        23351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_350_length_23351_cov_13.300137, whole genome shotgun sequence.
ACCESSION   DAOMVO010000006 DAOMVO010000000
VERSION     DAOMVO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 23351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23351
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_350_length_23351_cov_13.300137"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..2601)
                     /locus_tag="PKV30_00780"
     CDS             complement(196..2601)
                     /locus_tag="PKV30_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRL28784.1"
                     /translation="MQAISSQIANELQVREAQVQAAIDLLDGGATVPFIARYRKEATG
                     GLDDVQMRELEARLSYLRELHERRAAVLKSIESQGKLTPELRAAIDAAPNKQELEDLY
                     LPYKPKRRTKGMIAREFGIEPLADQLWGDPSLDPQEAAKLFVKAEKGEGGEDFTTTLA
                     VLDGVRDILSERWAEDAALVQSLREWLWAEGLLQSKLAAGKNENDPEVAKFRDYHDYD
                     EPIGRVPSHRALAVFRGRQLEILDAKLVLPEAESARAATKLAATPSLAEGRIALHLGW
                     SHQARPADDLLRKCVAWTWRVKLSLSTERDLFARLRDDAEKVAIKVFADNLRDLLLAA
                     PAGPRVVMGLDPGIRTGVKVAVCDATGKLVDTATVYPHEPRKDWDGSLHTLAKLVDKH
                     GVQLIAIGNGTASRETDKLAGDLIKLMQKHAPAQPGQAQAAIEKIVISEAGASVYSAS
                     EYASQELPDVDVSLRGAASIARRLQDPLAELVKIDPKSIGVGQYQHDVNQSELARTLD
                     AVVEDCVNGVGVDLNTASAPLLSRVSGLSGTVAKAVVRWREQHGAFKSRQQLLDVSGL
                     GPKTFEQSAGFLRIRGGDNPLDMTGVHPETYPVVEQIIIKTGKPIDQIMGRAELLKPL
                     KPDTFATAQFGAITVKDILTELEKPGRDPRPDFKVARFNDGVEDIADLREGMVLEGTV
                     SNVAQFGAFVDLGVHQDGLVHVSQLANKFVNDAREVVKTGQIVKVKVLEVDVPRKRIS
                     LTMKLDAQAPRRDAPRENRFENAGRGRGGYSSAPGGRGAGQTATGGAMASAFEKLKGL
                     QAK"
     gene            complement(2701..3936)
                     /locus_tag="PKV30_00785"
     CDS             complement(2701..3936)
                     /locus_tag="PKV30_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL28785.1"
                     /translation="MHPLALTGIVAAVAIVLMIQSLYAHGRGGDRLALPYAATLVCWL
                     ALLASNFWIDSGGLTGDGRALTRVLYQVTVLSVTAFLLLAMHGFFGLKLALTLLQAVL
                     GTVLALTDAMHEAWLWVNALLSSGLLLLLAQAIWRRPSSQGWMGLLVGISGVGVMVTD
                     LREAADGPIMISTSHYFFVVALFVLRMALMPRASVEATVRTQPAAQERQRLAQELHDG
                     VGSHLASIISALDLGTAQQRETAASLQHCMAELKLLVDGMDADASLLSHLASLRYRMQ
                     PLLAVAGIDLEWQIADEPALESVQGDAALQFLRLAQEALANVVRHSGANRVVLTCCHV
                     KAQQALMLEVADNGVGMPTGLRTVHPDTFSEGESLGKGMRSMARRAARLGGLLVVDGV
                     HGQGTRIRLLVPMARPAKP"
     gene            complement(3978..4721)
                     /locus_tag="PKV30_00790"
     CDS             complement(3978..4721)
                     /locus_tag="PKV30_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL28786.1"
                     /translation="MTRAPSSSVWPHFLTGQTDQPVRVIVVDDDAHMRRVISQELLAD
                     ARIHLVGQASSLREGKRLVSLHEFDVMLVDLNLGDGTGFQLIEHMKSVRPVAEAVVVS
                     AMEDEEHAMHAFEIGATGYLIKNSWFGSFGQAVLQVVNGGASITPNLARRLLKRMEGG
                     ARNGAAPGFQNHSAPSARPTPPGEKPSDREREILRLVASGHTSCEIGKRLGISDQTVN
                     THIKNIYRKLHVRTRAQAVNLASLRGWLY"
     gene            5287..6297
                     /locus_tag="PKV30_00795"
     CDS             5287..6297
                     /locus_tag="PKV30_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28787.1"
                     /translation="MKLLALFAMSAIASASAFAVTLSPAPQIVINGNSNQSTSVAAGA
                     DVHNHAYEKASALQNVSSNKGAVEINGNSNQSTSVTGTGSLRNHANNAGDIAIQNVSS
                     NYGVTEINGNSNQSATISAAVSNSARGAGEDCWGVDCRDTARALQNISSNFGNVKING
                     NSNQATTLTGGSASNEAVGAGAVAVQSMASNTGNVSINGNSNQSVALTRATVSNHAYG
                     KEASAVQNLASNYDGGYAGGVTINGNSNQSVGGTAGTIFNEADGNYAKAFQNLSSNMA
                     NVTINGNSNQSTVVNGTVWNKAEGRYSVAVQNLSSNDACEPPKFKLPDCPTGYCWGSR
                     PL"
     gene            6374..6784
                     /locus_tag="PKV30_00800"
     CDS             6374..6784
                     /locus_tag="PKV30_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28788.1"
                     /translation="MELNASCVAGKLVALAIAGACTCALAAPGEDPVQITSRVSYLQM
                     PTAPRAGSTDIRHRSGMPEDVRAKMAHYTAKAYSSDTTGILTEKDVVSSVKTDGMGAT
                     TCVQTVQPAAAGSERARSGDQVVVIRGDLINICR"
     gene            6918..8630
                     /locus_tag="PKV30_00805"
     CDS             6918..8630
                     /locus_tag="PKV30_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRL28789.1"
                     /translation="MNSCNSTLHSEPSRLGDRRKWLVTTMVTAALLTGCATPFDARKD
                     TEYQQRASVADRPVVRPIRSVSSFSESLTCMDRMLREAQLPTTLITSKQFVDFSGKAP
                     VATKELVVTALSQMSRLSHAFRYVDFEVDIARQDTVQNLTTILLNNNQMQLQRPALYV
                     SGAIAFVDQSVINNQAEAGLSGPRVDLGYSRSRNATIIGLEVHLGDFRTRTIIPGLDS
                     ANEVVTGGGGQGMDLGGRIGTYGVRFNVGRDYQLGTGAAMRTLVDLAMIELIGKWARV
                     PYWQCLTLDQTHPHFQRQMQDWYTESGPSGQFNLVKASLKSRGYLATDSESMAIDSPV
                     VRGALAKFQADRGMPVSGVVDLPTYVAALSDFVALSADGSFTRIGWSSSGPQQVATAD
                     GSTAPALGLTTAAPWMIDLQIENPQPAGERPAFTEGEQIFLSAMVSRASHLYCYYTDS
                     SGGVMRVLPNALQPHSLVSANQAIRIPDWMAPNPGFILDAGKPGTEAMICVATSQDAE
                     PHLPAEMRVPALSPIRGVKGVEGVQQVFGTALGGTNTHVSQVMRWQVAPRRQAQAVPK
                     TQTR"
     gene            8803..9933
                     /locus_tag="PKV30_00810"
     CDS             8803..9933
                     /locus_tag="PKV30_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28790.1"
                     /translation="MVAQVDIQLPAPARIESPSGAIPRIEPAPLGTTRIQPRPVDEAL
                     RITAPPLVTGSLGSRDQRASVLDRELQRLLRQAEAPRNYGSTPASAQAAWQLGLIHLH
                     GAGVRRDTALAQQWFARAARFGREPWAHAGLAWCALDGCSGPPDPAGAARAIAQLRQV
                     HPGRADFLAWVLASRQAPLQVVRPGQMQDQVLELPARQLLERAAAAGDVHANIELGMN
                     AVANDRIAQAEDYFRRAGPNSAAALADLSELRARGVSPVSPPVAPPSASANEALAAAR
                     KYHRGEGVPSNFVEAIRFYRLAESRGSVEARRMLGLIYSRPAPDGSLNAGWMQQLAYV
                     DTATVVPTVGVTGSNHMLLREPTPLFDLLPSFWRQQMTQVDR"
     gene            complement(9944..10780)
                     /locus_tag="PKV30_00815"
     CDS             complement(9944..10780)
                     /locus_tag="PKV30_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL28791.1"
                     /translation="MILHITDTHFGAERAEVVRGLIELARAQRPSLVVLSGDVTQRAR
                     RAQFDAARAFVDELCAACGIDAQRDVLIIPGNHDIPLFNLPLRVLDPYGHYVRAFGKR
                     LEPLIRRPDVWLIGVNTTRPWRHKHGELSSAQIERVAARLRAAPAGALRVVVTHQPLM
                     VVEQEDRRDLVRGHAAAIRAWAGAGADLFLSGHTHQSFVAPVLGAKRRAWVVQTGTAV
                     SSRVREHVPNSVHLIEWAGDASRHGWTVERLDWAGERFATVARTVIPPPADDRSLVPE
                     MV"
     gene            complement(10777..11718)
                     /locus_tag="PKV30_00820"
     CDS             complement(10777..11718)
                     /locus_tag="PKV30_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRL28792.1"
                     /translation="MSAAAPPLFFIFNAAAGHDDTRAIVQHVAALLRAAGRPAELLVA
                     GQPGELGALIGRAINGARDARGVVVAVGGDGTLNAVAQAVWNAGLVMGVLPQGTFNFF
                     GRVNGMPTDIGAAVDALLNARIEPVPVGMVGDRLFLVNASVGMYPQLLEEREQAKRRY
                     GRSRLVALWSGLLTLLRGGGVMTLELQGGADTRVVRTRTLFVGNNELQLRAVGLREAP
                     EVEAGRLAAITLQPLSKLRTLWMIVRGVMGRLDGAQGVDSFAFDRLVVQPRRRAASAK
                     VAIDGEIFRLPTPLIFRIAPRPLQLLVPAHQDGEGAP"
     gene            complement(11876..12787)
                     /locus_tag="PKV30_00825"
     CDS             complement(11876..12787)
                     /locus_tag="PKV30_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL28793.1"
                     /translation="MINDFKGKTAVLTGAGSGFGLECARIAARRGMNVVLVDIQADAL
                     AAAEAEIKALGARTMARRVDVSKPPVMELLAQHVKEEFGAPHFVFNNAGVGAGGLIWE
                     NTVKDWEWVLGVNLWGVINGVRGFTPMMLDEARRNPAYQGHIVNTASMAGLLTPPNMG
                     IYNVSKHAVVALTETLYQDLKLVTDQISASVLCPYFVTTGIHQSERNRPADMPAAELT
                     PSQRIQQAMTDKATGSGKVSAADVANKVFDAIEHDQFYVFSHPKALGNVKSRMDAIVE
                     IRNPPDPFAERPEIGVGLRAALRGEAA"
     gene            complement(12832..14493)
                     /locus_tag="PKV30_00830"
     CDS             complement(12832..14493)
                     /locus_tag="PKV30_00830"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRL28794.1"
                     /translation="MTATTHEVFNQVTPLADVNLFTGNAALQDALKFNAPGLNTTPLA
                     QLGQRVGSAEMQRDARLANAHTPELRTHDRFGHRIDEVEFHPSYHALMAAATEAGLHG
                     TPYADAEQASGHAHVLRAAGFMLFTELEPAVLCPISMTYAVTPALRDNPAIYRDWAPQ
                     LTARAYDPALKIWRDKPGVTMGMGMTEKQGGSDVRANTTRAVHEGRDDWGERYRLTGH
                     KWFFSAPMCDAFLVLAYVEGMGDGGLSCFFLPRVLPDGTRNVLRIQRLKDKLGNKANA
                     SSEVEFDGTVAWLVGEPGRGVPQILAMGTMTRLDCALGTSGLMRQALSIALHHTAERS
                     AFGKRLIEQPLMKNVLADLAIESEAACALSMRLARAFDHQADERERLLARVLTPIAKF
                     WICKRGSLFAQEAMECLGGNGYVEEGGEGIAARIYREMPVNSIWEGAGNIMALDLLRA
                     LRKGDVAGALADELAPARGTHAALDRMIDTLPTLIEHHATEASARLLARDVALTVQGA
                     LLAQSAPPAVVGAFCDSRLASRHDVFGTLGAGADLDALVAREQLA"
     gene            complement(14503..16221)
                     /locus_tag="PKV30_00835"
     CDS             complement(14503..16221)
                     /locus_tag="PKV30_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRL28795.1"
                     /translation="MSAAITSERALIDAAEQQGLLPPEARAAWHDEAGSSWIVIVLTF
                     LGAQLVVLPFLGFLALLSFEFFLRPPGAFVASAMLIGGAVVLLRARPGMFAAQLAFSG
                     LMAGLALLVVAFEGALGNAQLLIIFGVLIGAALLVRVTWVQRVLGFLAALVLLAVALW
                     PLGAGAHLDEWQQWEWRFLAFPMTLNAVLLGLLWGLWVVRERAWSGRAASSALHALAD
                     GAGVALLVAVAYGSTRHFWTLGMLVSGPMGSADAPWAGSARIFRLGWPALLQMALMTT
                     SAFWLLRHWRLRESMPTRDLVLLALAYAVLLVGCLVIPHIGVVALVGTLALGSGRRLI
                     LGLALATLLAQLSGFYYALAWPLAYKAALLAGMGAVLGLTLWAMRERTAPGVSTVAPL
                     PSFGPRWLTPVLVAAGAVLALGMVHIDVQNKEQVIAQGQKIYVPLAPRDPRSLMQGDY
                     MALNFAFPQEVRDALNKGDTLGTSRHALVVAVLDERGVATVRRLAQSNEALSAGERLL
                     PLKRLKDDWVLVTDAFYFPEGQGTPFARARFGEFRVLPDGRALLVGLADEHLQALLAA
                     PNPPAR"
     gene            complement(16218..17195)
                     /locus_tag="PKV30_00840"
     CDS             complement(16218..17195)
                     /locus_tag="PKV30_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRL28796.1"
                     /translation="MHALIHHLSHRFALDADRRQRLWALSGLHRPPDRLGVLLYRGLA
                     VLAALLLGAGLIFWVAANWQDQTRTFKLHLLEAAVLVSSVAALAWPRGRTALLLLATL
                     AMGGLLAFVGQTYQTGADAWQLFATWAALALLWALAARRDAMWALWLLITGLGLALWS
                     GDALLNPLQNLLIGRQARSLLTPLGWAAIFLLPLALPSLKLLPAGAASAPISWRLAAL
                     LALSAWCTYALFGLFLPDQAWQYFANVLLVMGAAALAWWLRPRDFVVLGLAVLALNVL
                     IISGFARLMFEGVRVDGVGAVLLITLVAAGCVGASGTWLYRLQREEATA"
     gene            complement(17337..18353)
                     /locus_tag="PKV30_00845"
     CDS             complement(17337..18353)
                     /locus_tag="PKV30_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRL28797.1"
                     /translation="MPSNRQILLDNRPEGEATTGNFKLVTTDTPALKDGEVLVRNHYL
                     SLDPYMRGRMNDSKSYAASQPLGEVMIGGTVGEVAESQNPKFAVGDKVVGMGGWQEYS
                     VVDGNAPGMLRKVDTTHVPLSFYLGAVGMPGVTAWYGLVKIIAPKPGATVVVSAATGA
                     VGSAFAALAKARGLRVVGIAGGPEKCKYAVEELHFDECIDYRQHPDVKTMSKALKDAC
                     PNGIDGYFENVGGYILDAVLLRTNAFARIALCGMIAGYDGQPLPLQNPALILVNRLTI
                     EGFIVSEHMEVWPEALKELGTLVATGKLKPRETIAEGIESAPEAFLGLLKGKNFGKQL
                     VKLI"
     gene            complement(18437..18877)
                     /locus_tag="PKV30_00850"
     CDS             complement(18437..18877)
                     /locus_tag="PKV30_00850"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL28798.1"
                     /translation="MSLDFGAVIPFVHHLGFTLERFEGGESTLHFAPRPEHCNSFGVA
                     HGGAVMTFLDVIMATAARSANAGSGIVTIEMKTSFMRPAPASVHGEPLVGKGRLVHRT
                     RTMAFAEGAIYDATGQLCATASGTFKYVPAAERPGDGKGPLPTD"
     gene            complement(18885..19676)
                     /locus_tag="PKV30_00855"
     CDS             complement(18885..19676)
                     /locus_tag="PKV30_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL28799.1"
                     /translation="MTRTIQQLFDLKGKTALVTGGSRGLGLQMAHALGEAGARIMLSS
                     RKADELEKAVADLEAAGIDARWTAADCAVEADIHRLADETLERMGDIDILVNNAGAAW
                     GAPAEDHPLDAWDKVMNLNVRGYFLLSQRVAKKSMIARKQGRIINIASIAGLGGNPPE
                     MQTIAYNTSKGAVVNFTRALACEWGHYGITVNAICPGFFPSKMTRGTLENLGEEKLAA
                     HAPLKRLGDDEDLKGITLLYASDAGKHITGQWLAVDGGVSVVTGG"
     gene            complement(19696..19998)
                     /locus_tag="PKV30_00860"
     CDS             complement(19696..19998)
                     /locus_tag="PKV30_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRL28800.1"
                     /translation="MLKHIVMWKFKPEAEGADKAANLVRAKALLDACAGLTPGMRMLE
                     VAIAQPGLECTYDLVLYAEFDDAAALDAYQNHPQHLAVKPFIGAVRLERQCMDYQT"
     gene            complement(20000..21829)
                     /locus_tag="PKV30_00865"
     CDS             complement(20000..21829)
                     /locus_tag="PKV30_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRL28801.1"
                     /translation="MALRPLLEFLLYDWLKAETLTQRQRFAEHSRETFDAVMDTCERI
                     AREKYAPFNRLVDTQEPHTETETDGSLRVVLPQATHDARAAYAESGMLAAAQDHEHGG
                     MQLPYLVEAAANAFFGKASISIGSNLLTVGNANLLMVHGTELQKQVFAANEFSGRWAG
                     TMCLSEPQAGSSLSDITTRAVLEDEGDPLGPRYRLRGNKMWISSGEHELTENIVHLVL
                     AKIPGPDGKLIPGVKGISLFIVPKRLVDTEGQLTGVRNDVALAGLNHKLGWRGTTNTL
                     LNFGEGQFKVDGQSGAVGYLVGEPHQGLRCMFHMMNEARIAIGMAAAMLGLAGYEASL
                     DYAKTRTQGRPVGAAGKDASQPPVRLIEHADIKRMLLAQKAYCEGALALLLYCARLVD
                     EQHTGDPAQADEARLLLEVLTPIAKSWPSENCLEANSLAIQIHGGYGYTRDFPVEQYW
                     RDNRLNMIHEGTHGIQAMDLLGRKVLMENGRGLQLLAGRVNATIERAIQQPTLAPHAN
                     ALAKALARIGAVTKAAWATGNPSEALANAVPYMQAFGHVVIAWTWLDLALAVDASKDE
                     AHRAGMAGAVAYFYQYELPKIDAWLGVVESRDMTCANMPEAAF"
     gene            complement(21876..22094)
                     /locus_tag="PKV30_00870"
     CDS             complement(21876..22094)
                     /locus_tag="PKV30_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28802.1"
                     /translation="MKTPMADSPSPIKQVLSQQAVLAVLDRWDDARTMMLNFWHSNPH
                     LASQGGERVRQLLMPLNEVQRLHAGDLA"
     gene            complement(22075..22575)
                     /locus_tag="PKV30_00875"
     CDS             complement(22075..22575)
                     /locus_tag="PKV30_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28803.1"
                     /translation="MKTSELLAALLDAHVDAVIVGGLAMQMHGYMRMTYDIDLVLAMN
                     DENLTRFIEVAKRLGLAPIIPVPIDALKNAQQIDAWHREKGLLGFALRGPDLTASVVD
                     VLVRPEIAYDRLAAAAVEGELAGRRIKIASIEHLLEMKRAANRPKDQLDIAALEKIQR
                     GEDPNG"
     gene            complement(22604..>23351)
                     /locus_tag="PKV30_00880"
     CDS             complement(22604..>23351)
                     /locus_tag="PKV30_00880"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRL28804.1"
                     /translation="IYWSMLQTAEQVAKRYNISRDAMDEYGAGSQQKAVAAQAAGKFD
                     DEIAPITVMAGVADPVMGLRTKEVVCSKDEGTREGTTKEGISGIKPALPGGLITAGNA
                     SQFSDGAGACVVVSEEYASKNNLKPMGRFLGFAVAGCEPDEMGIGPVFAVPKVLNKLG
                     LKVSDIDLWELNEAFAVQVIYCRDKLGIPADRLNVNGGAIALGHPYGVSGQRLTGHAL
                     IEGKRRGAKRVAVTMCIGGGMGAAGIFEVL"
BASE COUNT         4148 a         7818 c         7373 g         4012 t
ORIGIN      
        1 gcggccgagc gccaggcgcc tgaccctgag ccgccacgca aggcgcggcc caaggccaag
       61 tcgccaacgc aagcgcgtcg cacgcgcgcc aagcccgcac ggctgcggct gagcggcgct
      121 gcttgaggcg cctgccccag caacgctact atttaagtca tagcgcctgc cgcaggccag
      181 acaagcgctg cagccttatt tggcttgtag ccccttgagt ttctcgaacg ccgaggccat
      241 ggcgccaccg gtcgccgtct ggcccgcacc gcgtccgccc ggcgcgctgg aatagccgcc
      301 gcgcccgcgc ccggcgtttt caaaacggtt ctcgcgcggc gcatcacggc gcggcgcttg
      361 cgcatcgagc ttcatggtca ggctgatgcg cttgcgcggc acgtcgactt caagcacctt
      421 cactttcacg atctgcccgg tcttgaccac ttcgcgcgcg tcgttgacaa acttgttggc
      481 caactggctc acgtgcacca gtccgtcctg gtgcacgccc aggtcgacga aggcaccgaa
      541 ctgcgcgacg ttgctcaccg tgccttccag caccatgcct tcgcgcaggt cggcaatgtc
      601 ttccacgccg tcgttgaagc gcgcgacctt gaagtcgggg cgcgggtcgc ggccgggttt
      661 ttcaagctcg gtgaggatgt ccttcaccgt gatggcgccg aattgcgcgg tggcgaacgt
      721 gtcgggcttc aggggtttga gcaactcggc gcggcccatg atctggtcga tgggtttgcc
      781 ggtcttgatg atgatctgct cgacgaccgg gtaggtctcg gggtgcacgc cggtcatgtc
      841 gagcgggttg tcgccgccgc gaatgcgcag gaagcccgcg ctttgctcga aggtcttggg
      901 gcccaggccg ctcacgtcga gcagttgctg gcgacttttg aaggcgccgt gctgctcgcg
      961 ccagcgcacc acggccttgg cgacggtgcc cgacaagccc gagacgcgac tcagcaaagg
     1021 cgcgctggcg gtgttcagat ccacgccgac gccgttgacg cagtcttcga ccaccgcatc
     1081 cagcgtgcgc gccaactcgc tctggttcac gtcgtgctgg tactggccga cgccgatgga
     1141 tttggggtcg atcttgacca gctcggccag cgggtcttgg aggcggcgcg caatgctggc
     1201 ggccccgcgc aggctcacgt ccacatcggg cagctcttgc gaggcgtatt cgctggcgct
     1261 gtagaccgag gcaccggctt cgctgatgac tatcttttca atagctgctt gcgcttgtcc
     1321 cggttgcgct ggcgcgtgtt tttgcattaa tttgatgaga tcgcccgcca gcttgtcggt
     1381 ctcgcggctg gcggtgccgt tgccgatggc gatgagttgc acgccgtgct tgtcgaccag
     1441 tttggccagc gtgtgcaggc tgccgtccca gtccttgcgc ggctcgtgcg ggtagacggt
     1501 ggcggtgtcg accagcttgc ccgtggcgtc gcagacggcc actttcacgc cggtgcggat
     1561 gccggggtcg agccccatga ccacgcgcgg gccggccggg gcagccagca gcaggtcgcg
     1621 caggttgtcg gcaaagacct tgatggctac tttttcggcg tcgtcgcgca ggcgggcgaa
     1681 caggtcgcgc tcggtggaca gactgagctt gacgcgccag gtccaggcca cgcacttgcg
     1741 cagcaggtcg tcggcggggc gggcctggtg cgaccagccc aggtgcaggg cgatgcggcc
     1801 ttcggccagg ctcggtgttg ctgcaagttt ggtagctgct cgcgcagact ccgcctcggg
     1861 cagcaccagt ttggcatcca gaatctccag ctgccgccca cggaacacgg ccagcgcgcg
     1921 gtggctgggg acgcggccga tgggttcgtc gtagtcgtgg tagtcgcgga atttggcgac
     1981 ttcggggtcg ttctcgttct tgccggcggc cagcttgctt tgcaaaagcc cttcggccca
     2041 cagccattcg cgcaaggatt gcaccagcgc ggcgtcttca gcccagcgct ctgagaggat
     2101 gtcgcgcacg ccgtcaagca cggccaaggt ggtcgtgaag tcttcgccgc cctcgccttt
     2161 ctcagctttc acgaagagct tggcggcttc ttgtgggtca agcgaaggat cgccccacag
     2221 ctgatcggcc agcggctcga tgccgaattc gcgcgcgatc atgcccttgg tgcggcgctt
     2281 gggcttgtag ggcaggtaca ggtcttccag ctcctgcttg ttaggcgcgg cgtcgatggc
     2341 ggcgcgcagc tcgggcgtca gcttgccttg gctttcaatg cttttcagca ccgccgcacg
     2401 gcgctcgtgc agctcgcgca ggtagctcaa cctcgcttcc agctcgcgca tctgcacgtc
     2461 gtcgaggccg cccgtggcct ccttgcggta gcgggcgatg aagggcacgg tggcgccgcc
     2521 gtccagcagg tcgatggccg cctgcacctg ggcttcgcgg acttggagct cgttggcgat
     2581 ctgcgaggaa atggcttgca tgcatcaggg gctgcgcggg gttgaaaagg cgcgcagccg
     2641 cttccaaaaa aagctggcga gtttgccata cgggcgcctc cgtgatgccg tcaagaatcg
     2701 ttaaggcttg gcgggccgcg ccatcggcac cagcagccgg atgcgtgtgc cctggccgtg
     2761 cacgccatcg acgaccagca gcccgcccag gcgcgccgcg cgccgcgcca tgctgcgcat
     2821 gcctttgccc aggctttcgc cttcgctgaa ggtgtcgggg tgaaccgtgc gcagcccggt
     2881 cggcatgccg acgccgttgt cggccacttc cagcatcaac gcctgctgcg ccttgacgtg
     2941 gcagcaggtc agcaccaccc ggttggcgcc cgagtgccgc accacattgg ccagcgcctc
     3001 ttgcgccagg cgcagaaact gcagcgccgc atcgccctgc acgctttcca acgcaggctc
     3061 gtcggcaatc tgccattcca ggtcgatgcc ggccaccgcc agcaagggct gcatgcggta
     3121 gcgcaagctg gccaggtggc tgagcaacga ggcatcggcg tccatgccgt cgaccagcag
     3181 tttcagctcg gccatgcaat gctgcaacga ggcggcggtc tcgcgctgct gcgccgtgcc
     3241 cagatcgagc gctgaaatga tgctggccag gtgcgagccg acgccgtcgt gcagttcttg
     3301 cgccaggcgt tggcgctctt gggccgcggg ctgggtgcgt accgtggctt caacgctggc
     3361 gcgcggcatc aacgccatgc gcagcacgaa caacgcaacg acaaagaagt agtgcgaggt
     3421 cgagatcatg atcggcccat ccgccgcctc gcgcaggtcg gtcaccatca caccaacgcc
     3481 cgaaatgcca accagcaagc ccatccagcc ctgcgaagac gggcggcgcc agatggcttg
     3541 cgccaacagc agcagcagac cgcttgacaa caaggcgttg acccataacc aggcttcgtg
     3601 cattgcatcg gtcaacgcca ataccgtgcc cagcacggcc tggagcagcg tcagcgccag
     3661 cttgaggcca aaaaaaccgt gcatggcaag gagcaggaat gccgtcacgc tgagcaccgt
     3721 gacctggtac agaacgcgcg tcagcgcgcg accgtcgccc gtcaagccgc ccgaatcgat
     3781 ccaaaaattg gatgccagca acgcgagcca gcacaccagt gtggcggcgt aaggcagcgc
     3841 caaacgatcg ccgcctcgcc catgcgcgta caaggactga atcatcaaaa cgatggcaac
     3901 ggcggcgacg atgcccgtca gcgccagcgg gtgcatggtg tggcctcttt cgcggtgtcg
     3961 cgttgcgcga ccgccactca gtaaagccag ccgcgcagcg aggccaggtt gacggcctgc
     4021 gcacgcgtgc gcacgtgcag cttgcggtag atgttcttga tgtgcgtgtt gaccgtctgg
     4081 tcgctgatcc ctaagcgctt gccgatctcg caactggtgt ggcctgaagc gaccaagcgc
     4141 aggatctcgc gctctcgatc cgacggcttt tcgcccggcg gcgttggccg cgcggaaggc
     4201 gccgaatggt tttgaaagcc cggcgcagcc ccgttgcgcg caccgccttc cattcgcttc
     4261 agcagacgcc gcgccagatt cggggtgatc gacgcgccgc cgttgaccac ttgcagcacc
     4321 gcctgtccga agctgccaaa ccaggaattt ttgatcaggt agccggtggc gccgatctca
     4381 aacgcgtgca tcgcgtgctc ttcatcctcc atcgctgata ccaccacggc ttcagccacg
     4441 ggacgcacgc ttttcatgtg ctcgatcagc tgaaagcccg tgccgtcgcc caggttcaga
     4501 tcaaccaaca tcacgtcgaa ctcgtgcagc gacaccagtc gcttgccctc gcgcagcgaa
     4561 ctcgcctgcc cgaccaggtg aatgcgcgcg tcggccagca gttcttgcga aatgacccgg
     4621 cgcatgtggg catcgtcgtc caccacgatc acgcgcacgg gttggtccgt ctgccccgtc
     4681 aggaaatgcg gccacaccga agacgacggt gcgcgtgtca tgggtgtggc tgacgccact
     4741 tcgtctggca ccgagggtgc gcaagtagtt gtcaacgtgt tcattttcaa tcgcctcccc
     4801 gtgccggcag cgcatcgaaa catctgtaac gaagccacca gcgaaacatg aaatggtacg
     4861 aaccacaaaa cccaaacttt acatctttgg acatgttttt cgtgaactat gtccgcgacc
     4921 ataggcccgc aagccacatg gcctgagaac attttgatga ttttttctag ttactacaaa
     4981 tgacacgttt tgttactacc acacttacac atgcttaatg ctacggttat cagagcgttc
     5041 ggagacgact ggagtgcgcg atgcggcgaa acacaggggt ctctcagcct gttggcaccc
     5101 accgaacgac gtatccactt gtagcgattg gtgacgccgc gttcccggaa gacactgaac
     5161 cccgacaaag cagcaagctg agcatcgaaa aaacagggag gccggccgca gcagcgtcac
     5221 caccccggtt gaccggaatt acccgaatca accggacatg tgtcagtacc tttggaggaa
     5281 ctcatcatga aattgcttgc attgttcgca atgagcgcta tcgcatcggc atccgcgttt
     5341 gcggtgaccc tgtccccggc gcctcagatc gtcatcaacg gaaactcgaa ccaatccacc
     5401 agcgtggcgg ctggtgccga cgttcacaac catgcctacg agaaggcctc ggcgttgcag
     5461 aacgtctcca gcaacaaggg cgccgtggaa atcaacggga actccaatca atccaccagc
     5521 gtgaccggta ccggcagcct gcgcaaccat gcaaacaacg ccggtgacat cgcgatccag
     5581 aacgtctcgt ccaactacgg cgtgaccgag atcaacggca actccaacca aagcgcgacc
     5641 atcagcgccg ccgtgtcgaa cagcgcacgt ggcgccggtg aagactgctg gggcgtcgat
     5701 tgccgcgaca ccgcccgtgc attgcagaac atctccagca acttcggcaa cgtgaagatc
     5761 aatggcaaca gcaaccaggc caccacgttg accggtggct ctgccagcaa cgaggccgtg
     5821 ggagcaggag cagtcgccgt gcagtcgatg gccagcaata ccggcaacgt gtcgatcaac
     5881 ggcaacagca atcaatccgt cgcactgacg cgagccacgg tcagcaacca cgcctatggc
     5941 aaggaagcca gcgcggtgca gaacctggcc agcaattacg acggtggcta tgccggcggc
     6001 gtgaccatca acggcaactc gaaccaatcc gtgggcggca cggccggcac gatcttcaac
     6061 gaggccgacg gaaactatgc caaggctttc cagaacctgt cgtccaacat ggccaacgtc
     6121 acgatcaacg gcaactcgaa ccaatccacg gtggtaaatg gcacggtgtg gaacaaggcc
     6181 gagggacgct acagcgtggc ggtgcagaac ctgtcgtcca acgacgcgtg cgagccgccg
     6241 aagttcaagc ttcccgactg cccgaccggc tactgctggg gttccaggcc tctgtaatgg
     6301 aggaccgctg gctctggccg gttgactgcc ggccagggcc ttcgaaggtc aggttaaccc
     6361 aaggagaaca gccatggaac tcaatgcttc gtgcgtcgct ggaaaactcg tcgccctggc
     6421 gatagccggc gcatgcacat gcgccctggc agcaccggga gaagatcccg tgcagatcac
     6481 ctcccgggtc agttacctgc agatgcctac ggcaccgcgc gccggatcaa ccgatatccg
     6541 tcaccgtagc gggatgcctg aagacgtgcg cgccaagatg gcccactaca ccgccaaggc
     6601 gtattcctcc gatacgacgg gcatcttgac cgagaaagac gttgtctcat cggtcaagac
     6661 cgacggcatg ggcgccacca catgcgttca gacggtacaa cccgcggcgg ccggttccga
     6721 aagagccaga agcggcgatc aggtcgtggt gatccgcggt gacctgatca acatctgcag
     6781 atgacctgag ttcgaacgcc ggcggcgatg accctgagtc agggtttgca gccggctttt
     6841 ttcgctgcgg ttggcgacca tgtcccagcg aagtcgatga cgcactaaca gaactagatt
     6901 gacgcaaggg gaaggccatg aactcgtgca actcgacgct ccatagcgag cccagccgac
     6961 tcggtgaccg gcgcaagtgg ctggtgacca ccatggtgac cgctgccttg ctgaccggct
     7021 gcgctacacc gttcgatgcg cgcaaggaca cggaatacca gcaacgcgct tcggtggccg
     7081 atcggccggt cgtacggccg attcgatcgg tgtccagctt ctccgaatcg ctgacttgca
     7141 tggaccgcat gctgcgcgag gcgcagttgc ccacgacact gatcaccagc aagcagttcg
     7201 tggatttctc gggaaaagca ccggtggcta cgaaagagct ggtggtcacc gcgttgtcgc
     7261 agatgtcccg tctgagccat gcgtttcgat acgtcgattt cgaggtggat attgcgcggc
     7321 aggacacggt gcagaacctg acgacgatcc tgctcaacaa caaccagatg cagttgcagc
     7381 gaccggcgct ttatgtgtcg ggcgcgatcg ctttcgtcga tcagagcgtc atcaacaacc
     7441 aggcggaagc cggcctatcc ggaccgcgtg tcgatcttgg ttacagccgt tcgcgcaacg
     7501 ccaccatcat cggcctggaa gtccacctcg gcgacttccg cacgcggacc atcattccgg
     7561 gacttgattc tgccaatgaa gtggtcaccg gtggcggtgg ccagggcatg gacctgggcg
     7621 gccggatcgg cacctacggc gtgagattca acgtcgggcg cgactaccag cttggcacgg
     7681 gcgcggccat gcgaacactg gtcgacctgg cgatgatcga actgatcggc aaatgggccc
     7741 gcgtgcctta ctggcagtgt ctgacgctcg accagacgca cccccacttc cagcggcaaa
     7801 tgcaggattg gtacaccgaa agcggcccca gcgggcagtt caatctcgtc aaggcctcgc
     7861 tcaagagccg gggttacctc gccactgaca gcgagtcgat ggctatcgac agcccggtcg
     7921 tgcgtggcgc gctggccaag ttccaggccg atcgcgggat gcccgtgagc ggtgtcgtcg
     7981 atttgccgac ctacgtcgcc gcgttgagtg atttcgtcgc gttgagcgct gatggctcgt
     8041 tcacgcgcat aggctggagc agcagtgggc ctcagcaggt ggcgactgcc gacggcagca
     8101 ccgcgccggc gcttggcttg acgacggcag cgccatggat gatcgacttg cagatcgaga
     8161 atccgcaacc ggctggtgaa cgcccggcct tcacggaggg cgaacagatt ttcctatcgg
     8221 caatggtctc tcgtgcctcg cacctgtact gctactacac cgacagctcg ggcggcgtga
     8281 tgcgcgtgct acccaacgcg ctgcaacccc attcacttgt ttcggccaac caggccattc
     8341 gcatccccga ttggatggcg cccaatcccg ggttcatcct cgacgcgggg aagccgggaa
     8401 cggaagccat gatctgcgtg gcgacatcgc aagatgcgga gccccacttg cctgccgaaa
     8461 tgcgggtgcc cgccttgtct ccgatccggg gcgtgaaggg tgtggagggc gtgcagcagg
     8521 tgttcggaac tgctctgggt ggcacgaaca cccacgtgag tcaggtgatg cggtggcagg
     8581 ttgcacctcg acgtcaagcc caggcggtgc caaagaccca gacgcgatga tgtggcgcgc
     8641 gggcagacct gcagtcttga tcgcaatgtg gtcgccaacc agctgcctga aaggagcatt
     8701 accgtgccac gcgggtacat cactcccatg acgatagttg gcattgtcgc cggcgtggtg
     8761 ctgtactggc cacttcagcc ctgggcgcgt ccgagagcac tcatggtcgc tcaggtggac
     8821 atccaactcc ctgcgccggc gcgcattgaa tcaccgtccg gcgcgatccc gcggatcgaa
     8881 ccggcacccc tcgggacaac gcgtatccag ccgcgacctg tcgatgaggc acttcggatc
     8941 acggcgccac cgttggtgac gggcagtctc ggctctcgcg accagcgcgc cagcgtgctg
     9001 gatcgcgaac tgcagcgtct gctgcgccag gccgaggccc cgcgcaacta tggcagcacg
     9061 cccgcgtcgg ctcaggcagc ctggcagctg ggcctgatcc atctgcacgg cgccggcgtg
     9121 cggcgcgaca cggcactggc ccagcagtgg ttcgcgcgcg ctgccaggtt cggccgcgag
     9181 ccctgggctc atgcggggct ggcctggtgc gccctggatg gctgcagcgg accgcccgac
     9241 ccagcaggcg cagcgcgcgc aatcgcgcaa ctgcgccagg ttcatcccgg acgcgcggac
     9301 tttctcgcct gggtgctggc gtcgcgacag gcgccgctgc aggtggtgcg gccgggtcag
     9361 atgcaggacc aggtgctgga actgccggcg cggcagttgc tggaacgcgc cgcggcagcg
     9421 ggtgacgtgc acgccaacat cgaactgggc atgaacgcgg tggccaacga tcgcatcgca
     9481 caggcggagg attatttccg ccgcgctggc cccaattcgg ctgcggccct ggccgacctg
     9541 agcgaactgc gcgctcgcgg cgtgagtccc gtcagccccc ccgttgcgcc gccgagtgcc
     9601 agtgccaacg aggcgctggc ggccgcgcgc aaatatcatc gcggcgaagg cgtgccgtcc
     9661 aacttcgtcg aagcgatccg cttctatcgg ctcgccgaat cgcgcggcag cgtcgaggcg
     9721 cgccgcatgc tgggtctgat ttattcgcgg ccggcgccag acggcagcct gaacgcgggc
     9781 tggatgcagc aactggccta tgtggatacc gcaaccgtgg tgcccacggt gggcgtcacg
     9841 ggttccaacc acatgctgct gcgagagccg acgcccctgt tcgacctgct gcccagcttc
     9901 tggcgccagc agatgactca ggtcgatcgc tgacgccacg cgttcaaacc atctcaggca
     9961 ccagcgagcg atcgtcggca ggcggcggaa tcaccgtgcg cgcgacggtc gcgaagcgct
    10021 cccctgccca gtccaagcgc tcgaccgtcc acccgtggcg actggcgtcg cccgcccatt
    10081 cgatcaggtg cacgctgttg ggcacatgtt cacggactcg gctcgacacc gcagtgccgg
    10141 tctgcaccac ccaagcgcgg cgcttcgccc ccagaaccgg ggccacgaag gactgatgcg
    10201 tgtggccgct cagaaacagg tctgcaccgg cacccgccca cgcgcggatg gctgccgcgt
    10261 gcccgcgcac cagatcgcgt cgatcctcct gctcgaccac catcaaaggt tgatgggtga
    10321 ccacgacgcg cagcgcgccc gccggggccg cgcgcaggcg tgcggcaacg cgctcgattt
    10381 gcgccgacga cagctcgccg tgcttgtggc gccatggtcg cgtggtgttg acgccgatca
    10441 gccacacgtc cggtcggcgg atcagcggtt ccagcctttt gccgaacgcg cgaacatagt
    10501 ggccataagg atcgagcacg cgcagtggca aattgaacaa cggaatgtcg tggttgcccg
    10561 ggatgatcag cacgtcgcgc tgggcatcga tgccgcaggc agcacacagt tcatccacga
    10621 aggcacgtgc cgcatcgaac tgcgcgcgcc ttgcgcgctg cgtgacatcg cccgacaaca
    10681 ctaccagcga tggccgctgc gcgcgcgcca gttcgatcag gccgcgcacc acctccgccc
    10741 gctcggcacc aaagtgcgtg tcggtgatgt gcaggatcat ggcgcgccct cgccgtcttg
    10801 atgcgcgggc accagcagtt gcaaaggccg cggcgcgatg cggaagatca gcggcgtcgg
    10861 cagcctgaag atttcgccat cgatcgccac cttggcgctg gccgcgcggc ggcgcggctg
    10921 cacgaccagt cggtcgaagg caaagctgtc cacgccttgg gctccgtcca gccggcccat
    10981 cacgccgcgc acgatcatcc acagcgtgcg cagcttggac agcggctgca gggtgatggc
    11041 agccaggcga ccggcttcca cctcaggggc ttcacgcaag cccaccgcgc gcagttgcag
    11101 ttcgttgtta ccgacgaaca gtgtgcgcgt gcgcacgacg cgggtgtcgg cgccgccctg
    11161 caactccagc gtcatcacgc cgccgccgcg cagcagggtc aacaggcccg accacagcgc
    11221 gaccaagcgg ctgcggccat agcggcgctt ggcttgttcg cgctcttcca gcagctgggg
    11281 gtacatgccg acactggcgt tgaccagaaa cagccggtca ccgaccatgc cgaccggcac
    11341 cggctcaatg cgtgcgttca acaaggcgtc aacagccgcg ccgatgtcgg tcggcatccc
    11401 gttcacacgg ccgaagaagt tgaaggttcc ttggggcagc acgcccatca ccagccccgc
    11461 gttccacacc gcctgcgcga cggcgttcag cgtgccgtca ccgcccacgg ccaccaccac
    11521 gccgcgcgca tcacgggcac cattgatggc gcggccaatc agagcgccca gttcacccgg
    11581 ctgaccggct accagcaatt cggccggccg acccgccgca cgcaacaacg cagccacgtg
    11641 ctggacgatt gcgcgcgtat cgtcgtgacc agccgccgcg ttgaagatga aaaacagagg
    11701 gggtgctgcc gcagacatgg ctgcactgta gtggcggtgg cgcccggcgg ctgtcagcct
    11761 agggcgcaac cgtttgtcaa tgcgtcaaaa aacaagtcaa acaaggctct ggcgcccgtc
    11821 catcaagctc aagtagctat aaaaaaaata gtaaactaac ggttgatcga agtcctcacg
    11881 ccgcttcacc gcgcaatgcc gcgcgcaaac cgacgccgat ttcgggccgc tcggcaaagg
    11941 gatcgggtgg gttgcggatc tcgacgatcg cgtccatgcg tgacttcacg ttgcccagcg
    12001 ccttggggtg gctgaagacg taaaactgat cgtgctcgat ggcgtcgaac accttatttg
    12061 ccacatcggc cgcgctgacc ttgccgctgc cggtggcctt gtcggtcatg gcctgctgaa
    12121 tgcgctggct gggcgtgagc tcggccgctg gcatgtcggc cgggcgattg cgctcgcttt
    12181 ggtgaatgcc ggtggtcacg aagtacgggc acagcacgct ggctgagatc tggtcggtca
    12241 ccagcttcaa atcctggtac agcgtttcgg tcaacgcgac caccgcgtgc ttgctgacgt
    12301 tgtagatgcc catgttgggc ggtgtcagca ggcccgccat gctggcggtg ttgacgatgt
    12361 ggccctggta ggccgggttg cgccgcgcct cgtcgagcat catgggcgtg aagccgcgca
    12421 cgccgttgat cacgccccac aggttcacgc ccagcaccca ttcccagtcc ttgacggtgt
    12481 tttcccagat caggccgccg gcgcccacgc ccgcgttgtt gaagacgaaa tgcggcgcgc
    12541 cgaactcttc cttgacgtgc tgcgccagca gttccatcac cggcggcttg ctgacatcga
    12601 cgcggcgcgc catggtgcgc gcgcccaggg ccttgatctc tgcctcggcc gccgccagcg
    12661 catcggcttg aatgtcgacc agcaccacgt tcatgccgcg ccgcgcggcg atgcgtgcac
    12721 attccagccc gaagccggaa ccggcgccgg tcagcacggc ggtcttgcct ttaaagtcgt
    12781 tgatcattca atcctccgtg gggccgcgct gcggccggtt tgcaaaaaat ttcaggccaa
    12841 ctgctcccgt gccaccagcg catccaggtc ggcgccagcg cccagggtgc cgaacacatc
    12901 gtgccggctg gccaggcgcg aatcacagaa cgcgcccacc accgcgggcg gcgccgattg
    12961 cgcgagcagc gcgccctgca ccgtcagcgc cacgtcgcgc gccagcaaac gtgcgctggc
    13021 ttcggttgcg tggtgctcga tcagcgtcgg cagcgtgtcg atcatgcggt cgagcgcggc
    13081 gtgcgtaccg cgtgccggcg ccagttcgtc ggccagcgcg ccggccacgt cgcccttgcg
    13141 cagcgcacgc agcaaatcga gtgccatgat gttgccggcg ccctcccaga tgctgttgac
    13201 cggcatctcg cggtagatgc gcgcggcaat gccctcgccg ccctcctcca catagccgtt
    13261 gccgcccagg cattccatgg cttcttgcgc aaacaggctg ccgcgcttgc agatccagaa
    13321 cttggcaatg ggcgtcagca cgcgcgccag caaacgctcg cgctcgtctg cttgatgatc
    13381 aaaagcacgc gccaggcgca tggacaaagc gcaagcagct tccgattcaa tagcaagatc
    13441 ggccagcacg tttttcatca agggctgctc gatcaggcgt ttgccgaacg cgctccgctc
    13501 ggccgtgtgg tgcagcgcga tcgacagcgc ctggcgcatc aagccgctgg tgcccaaggc
    13561 gcagtcgagc cgcgtcatgg tgcccatggc cagaatctgt ggcacgccgc gccccggctc
    13621 gccgaccagc caggccacgg tgccgtcgaa ctcgacctcg ctgctggcgt tggccttgtt
    13681 gcccagcttg tctttcaggc gctgaatgcg cagcacgttg cgcgtgccgt caggcaacac
    13741 gcgcggcaga aagaagcacg acaggccgcc gtcgcccatg ccctcgacat acgccagcac
    13801 cagaaacgca tcgcacatcg gcgccgagaa aaaccatttg tggcccgtga ggcggtagcg
    13861 ctcgccccaa tcgtcgcgcc cctcgtgcac ggcgcgcgtg gtgttggcgc gcacgtcgga
    13921 gccgccctgc ttttccgtca tgcccatgcc catggtcacg ccgggtttgt cgcgccagat
    13981 cttcagcgca gggtcgtagg cgcgggcggt gagttgcggc gcccagtcgc ggtagatggc
    14041 cgggttgtcg cgcaatgccg gcgtgacggc gtaagtcatc gagatcgggc acagcaccgc
    14101 cggctccagc tcggtgaaca gcatgaagcc ggcggcgcgc aggacgtgtg catggccgct
    14161 ggcttgctcg gcgtcggcgt agggcgtgcc gtgcaggccg gcctcggtcg cggccgccat
    14221 cagcgcgtgg tagctggggt gaaattccac ctcgtcgatg cggtggccga agcggtcgtg
    14281 cgtgcgcaac tcgggcgtgt gcgcgttggc caggcgcgcg tcgcgctgca tctccgcgct
    14341 gccgacgcgc tggcccagtt gcgcaagcgg cgtggtgttc aggcccggcg cgttgaactt
    14401 caacgcgtct tggagtgccg cgttgccggt gaacagattc acgtccgcca agggcgtgac
    14461 ctggttgaag acttcgtgtg tcgtcgcagt catgtttttt cctcaacgcg ccggtgggtt
    14521 cggcgcagca agcagtgctt gcaaatgctc atccgccagc ccgaccagca gggcgcgacc
    14581 gtcgggcagc acgcggaact cgccaaagcg ggcgcgtgcg aacggcgtgc cctgcccttc
    14641 agggaaataa aaggcatcgg tcaccaaaac ccaatcatct ttcagacgct tgagcggcag
    14701 caagcgctcg ccggcggaca aggcctcatt gctttgagcc aggcgccgca ccgtggccac
    14761 gccgcgctcg tccagcaccg ccaccacaag ggcgtggcgc gatgtgccga gcgtgtcgcc
    14821 cttgttcagc gcgtcacgca cttcctgcgg aaacgcgaag ttgagcgcca tgtaatcccc
    14881 ctgcatcaac gagcgcggat cgcgcggcgc cagcggcacg tagatcttct ggccctgggc
    14941 aatcacctgc tctttgtttt gcacatcgat atgcaccatg ccgagcgcca agactgcgcc
    15001 agcagctacc aaaacaggag tcagccagcg tggcccgaag cttgggagcg gggcgaccgt
    15061 gctcactccg ggcgcggttc gctcgcgcat cgcccacagc gtcagaccca gcacggcgcc
    15121 catgcccgcc agcaacgccg ccttgtacgc cagcggccag gccagcgcgt aataaaagcc
    15181 cgaaagctgt gccagcagcg tcgccagcgc caaacccaga atgagccggc gaccactgcc
    15241 cagcgccagg gtgcccacca aagccaccac gccgatgtgc ggaatcacga ggcagcccac
    15301 cagcagcaca gcgtaggcca gcgccagcag gaccaggtcg cgcgtaggca tgctttcgcg
    15361 caggcgccag tggcgcagca accagaatgc gctggtcgtc atcagcgcca tttgcagcaa
    15421 tgcaggccag cccaggcgga agatgcgtgc gctgccggcc catggcgcgt cggccgagcc
    15481 catggggcct gaaaccagca tgcccagcgt ccagaaatgg cgcgtgctgc cataggccac
    15541 ggcgaccagc agcgccacgc cagcgccatc ggccagcgca tgcagcgccg aagatgcggc
    15601 gcggcccgac caggcccgct cacgcacgac ccacaggccc cacagcagcc ccagcagcac
    15661 ggcgttgagc gtcatgggga aagccaaaaa acgccattcc cactgctgcc actcgtccag
    15721 atgtgcgccc gcgcccagcg gccacagcgc caccgccagc aacaccagcg ccgccagaaa
    15781 gcccagcaca cgttgcaccc acgtcacgcg aaccagcagc gcggcgccga tcagcacgcc
    15841 aaaaatgatc agaagttgcg cgttgcccag tgccccttcg aacgccacca ccagcagcgc
    15901 aagtccggcc atcagcccgc tgaatgcaag ctgtgctgca aacatgccgg gtcgcgcacg
    15961 cagcagcacg acggcaccgc cgatcaacat ggcggacgcc acgaaggcac ccggcggtcg
    16021 cagaaaaaac tcaaagctca gcagcgccaa aaagcccaaa aaaggcagca ccaccaactg
    16081 cgcgcccagg aaagtcaaca cgatgacgat ccaggacgag cctgcctcgt catgccacgc
    16141 ggcacgcgct tcgggcggca gcaagccctg ctgctcggcg gcgtcgatca gcgcgcgttc
    16201 ggatgtgatg gcggcgctca tgcggtggct tcctcgcgtt gcaggcggta cagccaggtg
    16261 ccgctcgcgc ccacgcaccc ggcggccacc agcgtgatca acaacactgc gccgacgccg
    16321 tcgacacgca cgccttcaaa catgagccgc gcaaaaccgc tgatgatgag cacgttcagc
    16381 gccagcaccg ccaggcccag caccacgaag tcgcgcggcc gcagccacca ggccagcgca
    16441 gcggcgccca tcaccagcaa cacattggca aagtactgcc acgcctgatc gggcagaaac
    16501 aagccaaaca gcgcataagt gcaccacgcg ctgagcgcca gcaaggcggc caggcgccaa
    16561 ctgatggggg ccgacgccgc acctgcgggc agcagcttca agcttggcag cgccaacgga
    16621 agcaagaaga tggcggccca gcccagcggc gtcagcaggc tgcgcgcttg gcggccgatc
    16681 agcaggtttt gcagcggatt gagcaacgca tcgcccgacc acagcgccag ccccagcccc
    16741 gtgatcaaca accagagcgc ccacatcgcg tcgcgccgtg ccgccagcgc ccacagcagt
    16801 gccagcgcgg cccaggtggc gaacagctgc caggcgtccg cgccggtctg gtaggtctgg
    16861 ccgacgaagg ccagtagccc gcccatcgcc agcgtggcga gcagcagcaa ggcggtgcga
    16921 ccgcgcggcc aggccagcgc cgccacgctg ctcaccagca ccgcggcttc cagcaaatgc
    16981 agtttgaagg tgcgcgtctg atcctgccag ttggccgcca cccagaagat caagcccgcg
    17041 cccagcagca aggccgccag gaccgcgagg ccgcggtaga gcagcacacc caaccgatcc
    17101 ggcggacgat gcaagcccga aagtgcccac aggcgctgcc tgcgatcagc atcgagcgcg
    17161 aagcggtggg acagatggtg aatcagcgcg tgcaagagtg gctccggttg aaacagaccc
    17221 gctcattgtg cgcagcgacg cacgcaaacc ttggccactt catcgagaag tcgcgcgtgg
    17281 cgataccgat gggcgcatgt tcaggatttc accacgctgc gtgcaccgcg ccaccgtcaa
    17341 atcagcttga ccaattgctt gccgaaattc ttgcccttga gcagaccgag gaaagcctcg
    17401 ggcgcgcttt cgatgccctc tgcaatcgtc tcgcgcggct tcagcttgcc ggtggcgacc
    17461 agggtgccga gttccttcag cgcctcgggc cacacctcca tgtgctcgct gacgatgaag
    17521 ccttcaatcg tcaggcggtt gaccaggatc agcgccgggt tctgcagcgg cagcggctga
    17581 ccatcgtagc cggcaatcat gccgcacagc gcgatgcgag cgaaggcgtt ggtgcgcagc
    17641 agcacggcgt ccaggatgta gccgccgacg ttctcgaagt agccgtcgat gccgttgggg
    17701 cacgcatcct tcagcgcctt ggacatggtc ttgacgtccg ggtgttggcg gtagtcgatg
    17761 cactcgtcaa agtgcagctc ttccaccgcg tacttgcact tctcggggcc gcccgcaatg
    17821 cccaccacgc gcaggccgcg cgccttggcc agcgcggcaa aggcgctgcc cacggcaccg
    17881 gtggcggcgc tgacgaccac agtggcgcct ggcttcggcg caatgatctt caccaggcca
    17941 taccacgccg tcacgcccgg catgccgacg gcacccaggt agaacgacag cggcacgtgc
    18001 gtggtgtcga ccttgcgcag catgccgggc gcgttgccgt cgaccacgct gtattcctgc
    18061 cagccgccca tgccgacgac cttgtcgccc accgcgaact tggggttctg gctctcggcc
    18121 acttcgccca ccgtgccgcc gatcatcact tcgcccagcg gctggctggc ggcgtagctc
    18181 ttgctgtcgt tcatgcggcc gcgcatgtac gggtccaggc tcaggtagtg gttgcgcacc
    18241 agcacttcgc cgtctttcag cgcgggggtg tcggtggtga ccagcttgaa gttgcctgtg
    18301 gtcgcttcgc cttcggggcg gttgtcgagc aggatctggc ggttgctggg catggtgatt
    18361 tcctttcaaa aactacaaat tagatagctg cttgcgcttg tctgacaagc gctgcagcct
    18421 gttttgatct aaaaactcaa tccgtcggca acgggccctt gccatcgccc ggccgttcgg
    18481 cggccggcac gtacttgaag gtgccgctgg ctgtggcgca caactggccg gtcgcatcgt
    18541 agatggcgcc ttccgcaaac gccatggtgc gcgtgcggtg caccagacgg cccttgccaa
    18601 ccagcggctc gccgtggacg gatgccggcg ccggccgcat gaagctggtt ttcatctcga
    18661 tggtgacgat gccgctgccg gcgttggcac tgcgcgctgc cgtagccatg atcacgtcca
    18721 gaaacgtcat caccgcgccg ccgtgcgcca cgccgaacga attgcaatgc tccgggcgcg
    18781 gcgcgaaatg cagcgtggat tcgccgccct cgaagcgctc cagcgtgaag ccgaggtgat
    18841 gaacgaacgg aatgacggcg ccgaaatcaa ggctcatcgg tgcatcaacc cccggtcacc
    18901 acgctcacac cgccatccac cgccagccac tggccggtaa tgtgcttgcc ggcgtcgctg
    18961 gcgtacagca gcgtgatgcc cttcaggtct tcgtcgtcgc ccaggcgctt gagcggcgcg
    19021 tgcgcggcca gcttttcctc acccaggttt tccaacgtgc cgcgcgtcat cttgctgggg
    19081 aagaagcccg ggcagatggc gttcaccgtg atgccgtagt ggccccattc gcaggccagc
    19141 gcgcgcgtga agttgacgac cgcgcccttg ctggtgttgt aggcgatggt ctgcatctcc
    19201 ggcgggttgc cgcccagccc ggcgatgctg gcgatgttga tgatgcggcc ttgcttgcgc
    19261 gcgatcatcg acttcttggc cacgcgctgg ctcagcaaaa aatagccgcg cacgttgagg
    19321 ttcatcacct tgtcccaggc gtcgagcggg tggtcttcgg ccggcgcgcc ccaggcggcg
    19381 cccgcgttgt tgaccaggat gtcgatgtcg cccatgcgct ccagcgtttc gtcggccaag
    19441 cggtggatat cggcctccac cgcgcagtcg gcggccgtcc agcgtgcgtc gatgcccgca
    19501 gcttccaggt cggccacggc tttctcaagc tcatcggcct tgcgcgacga cagcatgatg
    19561 cgcgcgccgg cctcgcccag cgcatgcgcc atctgcaggc ccagcccgcg cgagccgccg
    19621 gtgacgaggg cggtcttgcc cttgaggtcg aagagttgtt ggatggtgcg tgtcatgatg
    19681 agttgctctg cgggttcagg tttggtaatc catgcattgc cgctccagcc ggacggcgcc
    19741 gatgaagggc ttgaccgcca ggtgttgcgg gtggttctgg taggcgtcga gggcggccgc
    19801 gtcatcgaac tcggcgtaca acaccaggtc gtacgtgcat tccagcccag gttgggcgat
    19861 ggcgacttcc agcatgcgca tgcccggcgt caggcctgca caggcgtcca gcagcgcctt
    19921 ggcgcgcacc aggttggcgg ccttgtcggc gccttccgcc tcgggcttga acttccacat
    19981 cacgatgtgc ttgagcatgt cagaacgccg cttcgggcat gttcgcgcag gtcatgtcgc
    20041 ggctttccac cacacccagc caggcgtcga tcttcggcaa ttcgtattga taaaaatagg
    20101 ccacagcgcc cgccatgcct gcgcggtgcg cttcatcttt tgaagcatca acagcgagcg
    20161 ccagatccag ccacgtccac gcaatgacga cgtggccaaa ggcctgcatg tagggcacgg
    20221 cgttggccag cgcctcgctc gggttgccgg tggcccaggc ggccttggtc acggcaccga
    20281 tgcgcgcaag ggccttggcg agcgcgttgg cgtgtggcgc cagcgttggt tgctgaatag
    20341 cgcgctcgat ggtggcgttg acgcgcccgg ccagcagttg caggccgcga ccgttttcca
    20401 tcaacacctt gcggcccagc aaatccatgg cctggatgcc gtgcgtgcct tcgtggatca
    20461 tgttcaggcg gttgtcgcgc caatactgct ccacgggaaa gtcgcgtgtg tagccgtagc
    20521 cgccgtggat ctggatggcg agcgagttgg cctccaggca gttctcgctc ggccaactct
    20581 tggcgatggg cgtgagcact tcaagcagca ggcgcgcttc atcggcttgc gcggggtcgc
    20641 cggtgtgctg ctcgtccacc agcctggcgc aatacagcaa cagcgccagc gcgccttcgc
    20701 aataagcctt ttgcgccagc agcatgcgct tgatgtcggc gtgttcgatc aggcgcaccg
    20761 ggggctgcga tgcgtctttg cccgccgcgc cgaccggccg accttgcgtg cgcgtcttgg
    20821 cgtagtccag cgatgcttcg tagcccgcca agcccagcat ggcggccgcc atgccaatgg
    20881 cgatgcgcgc ctcgttcatc atgtggaaca tgcagcgcag gccctggtgc ggctcgccga
    20941 ccaagtagcc cacggcaccg gattgaccat ccaccttgaa ctgcccctcg ccaaagttca
    21001 gcagcgtgtt ggtggtgcca cgccagccga gcttgtggtt caggccagcc agcgccacgt
    21061 cgttgcgcac gccggtcaac tgcccctcgg tatcgaccag cctcttcggc acgatgaaga
    21121 gcgaaatccc cttcacgccc ggaatcagtt tgccgtccgg gcctggaatt ttggccagca
    21181 ccagatgcac gatgttctcg gtcagctcgt gctcgcccga ggagatccac atcttgttgc
    21241 cgcgcaggcg gtagcggggc ccgagcggat cgccttcatc ttccagcacg gcgcgcgtcg
    21301 tgatgtcgct caaggacgag cccgcctgcg gctccgacag gcacatggtg ccggcccagc
    21361 gaccgctgaa ttcattggcg gcaaacacct gcttttgcag ttcggtgcca tgcaccatga
    21421 gcaggttggc attgcccacc gtcagcaggt tggagccgat gctgatcgac gccttgccga
    21481 aaaaggcatt ggccgccgcc tcgaccagat acggcagctg catgccgccg tgttcgtgat
    21541 cctgcgcggc ggccagcatg ccggattcgg cgtaagcggc gcgcgcatcg tgcgtggcct
    21601 gcggcagcac cacgcgcagg ctgccgtcgg tttcggtttc ggtgtgtggc tcttgggtat
    21661 cgacgagacg gttgaacggc gcgtacttct cgcgcgcaat gcgctcgcag gtgtccatca
    21721 ctgcatcgaa agtttcgcgc gagtgctcgg cgaagcgctg gcgctgcgtc agcgtttccg
    21781 ctttcagcca gtcgtagagc agaaattcaa gcaacgggcg cagcgccatg cggaaattcc
    21841 ctattttgtg aatcgcaaat tggcgccgcc ggcggctacg ccagatcgcc tgcgtgcagg
    21901 cgctgcactt cgttcagcgg catcagcagt tgacgcacgc gctcgccccc ttggctcgcg
    21961 aggtgcggat tgctgtgcca gaaattcagc atcatcgtgc gcgcatcgtc ccagcgatca
    22021 agcaccgcca gcacggcctg ttgcgaaagc acctgtttga tcgggcttgg tgagtcagcc
    22081 attggggtct tcaccgcgct gaatcttctc caacgctgca atgtcgagct gatccttggg
    22141 ccggttggcg gcgcgcttca tctccagcag atgctcgatg ctcgcgatct tgatccgacg
    22201 ccccgccagt tcaccttcca cggcagcggc ggccaaccgg tcgtaggcaa tttcaggacg
    22261 caccagcacg tccacgacac tggcagtcaa atcaggtcca cgcaatgcaa agcccagcag
    22321 gcccttctcg cgatgccagg cgtcgatctg ttgggcattc ttgagtgcgt cgatgggcac
    22381 tggaatgatg ggcgccaacc ccaaacgctt ggcaacttca atgaaacgtg tgaggttctc
    22441 gtcgttcatc gccagcacca ggtcgatgtc gtaagtcatg cgcatgtagc catgcatttg
    22501 catggccaaa ccaccgacga tcacggcatc gacgtgggca tccagcaatg ccgcaagcag
    22561 ttccgacgtc ttcatcacga ttgaccgaga ctcctggtcc ccgtcacagc acctcgaaga
    22621 tgcccgccgc ccccatgccg cccccaatgc acatggtcac agcaacgcgc tttgcgcctc
    22681 ggcgcttgcc ttcgatgagg gcgtggcctg tcaggcgctg gccgctcacg ccgtacgggt
    22741 ggcccagcgc gatggcgccg ccgttcacat tcaggcgatc ggcgggaatg cccagcttgt
    22801 cgcggcagta gatcacctgc acggcaaagg cttcgttcag ctcccacagg tcgatgtcgc
    22861 tgaccttcag gccgagcttg ttcaacacct tgggcacggc gaacaccggg ccgatgccca
    22921 tttcgtccgg ctcacagccg gcgacggcga agccgaggaa acggcccatg ggcttcaggt
    22981 tgttcttgct ggcgtattcc tcgctcacca ccacgcaggc gccggcgccg tcgctgaact
    23041 ggctggcgtt gccggcggtg atcagcccgc cgggcagcgc gggtttgatg ccgctgatgc
    23101 cttccttggt cgtgccttcg cgtgtgccct cgtccttgga gcaaaccact tccttggtgc
    23161 gcaggcccat caccgggtcg gcgacgccgg ccatcacggt gatgggtgcg atctcgtcgt
    23221 cgaacttgcc cgccgcctgc gccgccacgg ccttctgctg gctgccggcg ccgtattcgt
    23281 ccatcgcgtc gcgactgatg ttgtagcgct tggccacctg ctcggcggtc tgcagcatgc
    23341 tccagtagat c
//
LOCUS       DAOMVO010000007        22458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_375_length_22458_cov_15.475204, whole genome shotgun sequence.
ACCESSION   DAOMVO010000007 DAOMVO010000000
VERSION     DAOMVO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 22458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22458
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_22458_cov_15.475204"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PKV30_00885"
     CDS             <1..1314
                     /locus_tag="PKV30_00885"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRL28805.1"
                     /translation="LGPRETKAAIAAANQAWPAWRELTAKQRHAILMKWFDLLMANQD
                     DLGRLMTAEQGKPLPEAKGEVAYGASFVQWFAEEAKRAGGETLPTSDPTKRLMVLRQP
                     IGVCAAITPWNFPLAMITRKVAPALAAGCPVIIKPAELTPLTALAAAELAVRAGVPAG
                     VINVITTTDSSAVGKVLCESEVVRHLSFTGSTEVGRILMAQCAPTIKKLALELGGNAP
                     FIVFDDADIDAAVEGAIASKYRNAGQTCVCANRLYAQDGIYDKFVKKLAAKVAKMKVG
                     NGFDDGVVIGPLIEDAAVNKVARHVADAVNKGGKVLTGGEKMKGQFFEPTVISGATAD
                     MLVATEETFGPLAPVFRFKTEQEAIDAANATIFGLASYFYARDIGRITRVGEALEYGI
                     VGVNTGIISVEQAPFGGVKQSGLGREGSRHGLDEYLEMKYLCVGI"
     gene            1500..3044
                     /locus_tag="PKV30_00890"
     CDS             1500..3044
                     /locus_tag="PKV30_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRL28806.1"
                     /translation="MWLDDAGRPRAATREAVRWLTDAEQQGLRPQDYDASRLASAVAD
                     AERAAPSPDAAAQLDESLTRQVLAYLSDLRHGRVDPARIQARYDSATAPAPNLPAVLR
                     EAVAQGQLQDAQRAALPPWPQYADLQKALVHYRSLADHPAWGKALPALPGGKLQAGQR
                     WAGLATLAERLQALGDLPEAPATAVTAYDATLQKAVQSFQSRHALPADGVIGKSTLDA
                     LAVPPATRAQQIALAMERMRLTPLPAAPRFVTVNVPEFTLRAFRTDAGSVREDLQMRV
                     IVGKALDTRTPLFDEDMLWIEFSPYWNIPPSIARKETVPTLRRNPGYLAAQGMEFVSA
                     SGVSTDVTPQNLDAVLAGQMRIRQRPGPRNALGDIKFMLPNNQNIYLHHTPSPGLFSR
                     TRRDFSHGCVRIEEPVALAQWVLHDQPEWTEARIRQSMGRPRPVTAKLLEPVPVLMLY
                     QTAVVDGGKLHFVPDVYGQDAVLEKALKQPRPAPAATVTPVTRVPAAPQPVSARAGAS
                     DSIAAQ"
     gene            3086..3652
                     /locus_tag="PKV30_00895"
     CDS             3086..3652
                     /locus_tag="PKV30_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRL28807.1"
                     /translation="MQNPHRRLFLGRSFSAAKVAAAGVLLPGAARAFSPASTAGARRL
                     SFNHLHTNERTALVYAVGGDFVPRALDHLNHFLRDHYTQEVGQMDPELYNLLHAVRRE
                     LGTELPFDVISGYRSPHTNETLRTTRGGGVAKRSLHMDGKAIDVRLPGVALTDLRDAA
                     LSLKAGGVGFYESDNFVHIDTGRVRSWG"
     gene            complement(3742..5058)
                     /locus_tag="PKV30_00900"
     CDS             complement(3742..5058)
                     /locus_tag="PKV30_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRL28808.1"
                     /translation="MTPPTPQVLIIGAGPAGLMAAEVLSGAGVAVDVFDAMPSVGRKF
                     LLAGKGGLNLTHSEPLPAFIGRYGAQTDTVANWLGDFGPDATREWAAGLGVETFVGTS
                     GRVFPAEMKAAPLLRAWLQRLRHPSAGGAPVRFHMRHRWVGWQAEKTMNSVAASASPS
                     SAGGLFCFESPVGEVNVQPRVAVLALGGASWPRLGSDGTWMPWLQQMGADVAPLQPAN
                     CGFDAAGRAGDGWTPFFSERFAGQPLKNVSITFTNHAGQRFERRGEFVLTATGVEGSL
                     IYAASAALRDEIARAGSATFTLNLLPGRTAADVHAAVAHPRGSRSLSSQLKSRLGLAP
                     VHTALLHELLTAEQLKDAAALAQAIQALPITLRAPRPVAEAISTAGGVRLSALTEDLE
                     LRTAPGVFCAGEMLDWEAPTGGYLLTACLASGVRAARGVLRRLEHG"
     gene            complement(5354..8686)
                     /locus_tag="PKV30_00905"
     CDS             complement(5354..8686)
                     /locus_tag="PKV30_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRL28809.1"
                     /translation="MTTPAYEHNGAPCSRAAFYAIACDPRRSVAVEACAGAGKTWMLV
                     SRILRALLGGAAPQEILAITFTKKAAGEMRERLQQWLADFAAPRAAETPEAWQQRLDA
                     ELLARGIEPQRLPSQREQLQNLYQSLLAQGRPVQIRTFHSWFAALLRSAPLSVLQQLG
                     LPSTYELLEDDADAVAEVWRRYLRRVAGDADLSADYAALVATLGRHRAHAALEGALAR
                     RVEFLLADAAGHVDDAVEPFDVLYPRLAGVAQPADWLLQRAAGRALLQDAARALAPLA
                     RTYAAKGAELLAALEAEDWDGVVAALFTKNGTPRAFSKHTPGDVRAAQNEVEAVLAAE
                     AQHAARQYHLRMTRLTRPLIESFAELKRERGWVDMSDVERAALTLLSDPFLSGWVQER
                     LDARTRHLLIDEFQDTNPLQWQALHAWLSSYAGAGGGTSAPSVFIVGDPKQSIYRFRR
                     ADPQVFIAAQDFIVNALDGDRLACDHTHRNAPEVLGAVNTVMLAAQAADEFSGYRAHS
                     TESTARGHIGALPLIPRPDRAATGEGDEPVWRDSLTTARVALEDSLRTLESRQAARWI
                     ASRIAAGVPPDRLMVLARKRLPLGELQAELRQLGIACEQPEQQVLGEQAAVQDVLALV
                     DALVSPGHDLALARALKSPLFDLTDDDLVRIALAVRASTPSPPWGEGRGEGQPAPDTP
                     LPPRGTPPAWLEVLPKTELPTLDTHALAADLTQYRHWLLTLPPHDALQAIYSHRDVLA
                     RFAAAAPAPERAQVLAQLRALLAAALRVQGGRFLTAYQWLRALRRQRLPAPRPSAPQA
                     VQLLTVHGAKGLEADEVLLLDAASAAPRGGGPTTLIDWPGEQPAPTRLVFLASEDAPP
                     PGVADLAAQEAAAQAREELNALYVAMTRARGRLVLSGVTPYSQPASSWWQRIEALAEP
                     LPVPQDAVAPAGDEDSFAMLELPPALVQQAQEAIESEVAKAEPTDTSRVGEAMHWLLE
                     HAADTPQGWLPERVAQARRRFGADADQAARAETLARRILTGDAAWAWAGGEVLEAFNE
                     IELTHQGQRLRIDRLVRRRAGAHGDEAWWVLDYKSAARPERDALLVEQLGRYREAVSL
                     IHPGQTVVAAFLSADGRVVSVG"
     gene            complement(8847..11372)
                     /locus_tag="PKV30_00910"
     CDS             complement(8847..11372)
                     /locus_tag="PKV30_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRL28810.1"
                     /translation="MTAIAENDGATTTSGVLNAWQGLLARIDAELRQLAAHPARSVVL
                     LPYAQLMPLAARLWAAQFPDAFAPRFETTRNWATRAGWFTPGPHDLSFDHGRDLLTAA
                     ALLEGAGLAAQRALLAGPLVEQATQLGQIAASLPPALRPDWAEQARGALPPVGDGPLA
                     LEAAVGRIAIAWAGGSDYATDVLFAPRVADALDALLIVPGLLPDPLTETLAEHYFEKA
                     VLLHPESDAALGHIALHTCADAEDEAERAAACVLRQIEVGRVPVALIAGDRLLTRRIN
                     ALLIERGAQLRDETGWKLSTTHAAAQLMAALHACAPRASTDEVLDWLKLAPAFDARQQ
                     RALERRLRRDAVRGWPQAALLTGDQPLTQHIESLRVPMRAARPLADWLPALRTLLQGC
                     GLWPLLAADVAGNAVIDALGLSDEALADWRDWPAAQRRMGLAEFTRWVGEALEAASFR
                     PPHPAAAQVVVLPLTQLLGRPFASLVLPGADEQRLPAAPEPPGPWSAAQRQALHLPTR
                     EDLRDAQAAAWALALRVPQVDVLWRNTDDSGEPLLPSPLVQSLQLQELAALGAEGREA
                     RFIQIRPTERPQPRGDALPTQPLSASGYEMLRACPYRFFALRQLGLAEEGELDVDIDK
                     RDWGNWLHDTLRGFHEALRVAPDADRLTLIDAAAEAATEALGPGLEPGEFMPFAVAWP
                     TLRDAYLQWLAKHEAGGAQFKLAEESIDTQRGPLRLKGQIDRIDEVTDGATLLIDYKT
                     EPLARTRDRIKAGNEDTQLPFYALLSGADAPRAAYLNLAEREVPSLHELPELPMLAAQ
                     LYEGMAYDLARIAAGEPLPALGEGSVCGWCDARGLCRKDFWKT"
     gene            11476..11808
                     /gene="trxA"
                     /locus_tag="PKV30_00915"
     CDS             11476..11808
                     /gene="trxA"
                     /locus_tag="PKV30_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HRL28811.1"
                     /translation="MPNELIKHVSDSSFETDVLQSDKPVLVDFWAEWCGPCKAIAPTL
                     DELATAYDGKLQIAKVNVDENRAVPAKFGIRGIPTLMVFKNGQLSATKVGALTKAQLS
                     QFIDQQLA"
     gene            12041..13306
                     /gene="rho"
                     /locus_tag="PKV30_00920"
     CDS             12041..13306
                     /gene="rho"
                     /locus_tag="PKV30_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRL28812.1"
                     /translation="MHLNELKALHVSELLKQAETLEIDNAGRMRKQELMFALIKKRAK
                     AGEQVFADGVLEILPDGFGFLRSPDSSYTASTDDIYISPSQVRRFNLHTGDMIEGEVR
                     IPKDGERYFALTKLDMVNGSPAEQNKHKVMFENLTPLFPREQMRLERDAFKGEENITG
                     RIIDVIAPIGKGQRALIVAPPKSGKTVMMQTIAHAISANYPEAHMMVLLVDERPEEVT
                     DMQRSVKGEVIASTFDEPAARHVHVAEMVIERAKRLVELKKDVVILLDSITRLARAYN
                     NVVPSSGKVLTGGVDASALHRPKRFLGAARNVEEGGSLTIIATALIDTGSRMDEVIFE
                     EFKGTGNSEIHLDRRLYEKRVFPSIQLNRSGTRREELLLAPEVLQKTRILRQFMYNMD
                     EIEAMEMVIKSMKATKTNVEFFDMMRRGG"
     gene            13524..13775
                     /locus_tag="PKV30_00925"
     CDS             13524..13775
                     /locus_tag="PKV30_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRL28813.1"
                     /translation="MREGIHPDYREVLFVDLSNGFKFVTRSCVNTKEKETFEGKEYPL
                     YKLDTSSESHPFYTGTQKSVDNMGGRVEKFRNRFGKVAR"
     gene            13907..15619
                     /locus_tag="PKV30_00930"
     CDS             13907..15619
                     /locus_tag="PKV30_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28814.1"
                     /translation="MNPKPSPAIVTQDAARRLPRWALLLLCVAYVIPGYVGREPWKNA
                     DIASFGYMLALLDAGSWREWLQPSMMGLVPDNGALLPYWLGAWAMQALMPLGLAPEIA
                     ARVPYLLMLAGTLVATWYAVYHLARDPSAQPVAFAFGGEAEPVDYARALADGSLLALV
                     ATLGLAQFSHETTPALAQLFFGTLVFYGLAALPTRPLAPFIALLVGLPGLTLSGAPAM
                     AMIYAGLGLLALALPGAAPAHGAARLRALLQLLALVGLCLALALALDLFRWRIAPWRT
                     SGAEWSSLARLFLWFTWPAWPFVLWTLWRWRRQLLSLRQQRHLGLPLAIASVPLLGTL
                     LTLAGDRALLLALPALAALAALALPTFSRSVSALIDWFTVLFFTGWTLVIWVVWVAME
                     TGVPAKPAANVARLAPGFDPVFQWPAFVAALLGTLAWFALARWRTGRHRTALWKSMVL
                     PAAGTASCWLLLMTLWLPALDYGRSYAPQMREVRALIGDASCVEVHGLTEAQVAAVRF
                     HGGWLTVPARGPVACPWLLVDVDAQSSLTASVSMTDWREVGQVRRPTDRNETLYVLRR
                     VGRP"
     gene            complement(15789..17156)
                     /gene="phoR"
                     /locus_tag="PKV30_00935"
     CDS             complement(15789..17156)
                     /gene="phoR"
                     /locus_tag="PKV30_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HRL28815.1"
                     /translation="MALRLFLVALAHLALGLLGGWWLGWPGALAGVVLAAALTWGIDA
                     WRARRFLIWISKTNASTVPALRGAWGEAAYRTARALRAEQQQAQLSSQRLNDFLAAIQ
                     ASPNGVLLLDPNGRIEWCNDIAADHFGIDPQRDLLQHVGNLVREPAFSAYYASKNHER
                     EVKLIGRHNTPAHPVKLSVQLHPYGNGHQLMLSRDITALAQADAMRRDFVANVSHEIR
                     TPLTVLAGFVETLQSLPLSEEERAKYLDMMATQSDRMQTLVNDLLTLSRLEGSPLPGA
                     GEAIDLRALMTQCEAEARGLSNLMHPADERPQTLRFGAAPMFDLTGSANELRSAVSNL
                     TNNAVRYTPGGGLIEVQWQRLPDGGARLVVTDSGPGIAPQHLPRLGERFYRVDRSRSR
                     ESGGTGLGLAITKHVAQRHGGELQIRSELGHGSIFALEFPPQRVRAASTAALVPGSVA
                     PNAEA"
     gene            complement(17173..17907)
                     /gene="phoB"
                     /locus_tag="PKV30_00940"
     CDS             complement(17173..17907)
                     /gene="phoB"
                     /locus_tag="PKV30_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRL28816.1"
                     /translation="MRTLPRVLIVEDEAPIAELIAVNLRHNGFQPTWAMDSETAQREL
                     DAVLPDVILLDWMLPGESGLSLAKKWRKDARTKGVPILMLTARGDEADRVAGLDAGAD
                     DYITKPFSTKEMLARIRAVLRRRSPEQAGGSVAIGALLLDASTHRVSYDGQMLKMGPT
                     EFKLLHYFMKNAERVHSRGQLLDKVWGDHVFIEERTVDVHVKRLREALGVAGVLIETV
                     RGAGYRLTAQPMAQLQHGAPAAATSS"
     gene            complement(17904..18605)
                     /gene="phoU"
                     /locus_tag="PKV30_00945"
     CDS             complement(17904..18605)
                     /gene="phoU"
                     /locus_tag="PKV30_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRL28817.1"
                     /translation="MYEKHLSSQFDSELNRVSSNVLELGGLVEMQIRQAIYALSEFSI
                     EVADQVEKDEQRVNALELEIDRELSNIIARRQPTAIDLRLLLAISKTTANLERVGDEA
                     AKIARMVKRIIDSGKARMLPTGELRHAADLAAQQLRRALDAFARLSADDAVSIIRDDD
                     QVDREFDGFVRTLITYMMEDPRTISAALDLLTLAKAIERIGDHAKNIAEFIIYIVKGA
                     DVRHGTMEDLESVAR"
     gene            complement(18621..19442)
                     /gene="pstB"
                     /locus_tag="PKV30_00950"
     CDS             complement(18621..19442)
                     /gene="pstB"
                     /locus_tag="PKV30_00950"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRL28818.1"
                     /translation="MEVATRVPQASIRGNGVSFDQERLALEIKNLDFFYGNGFQGLKN
                     VNLSIPEHKATAFIGPSGCGKSTLLRTLNRMYSLYPGQRAVGEILLYGKNVLDPSVDV
                     NLLRAQVGMVFQKPTPFPMTIYENIAFGVRLYESLNKADMDDRVEWALSKAALWNEVK
                     DKLGQNGLSLSGGQQQRLCIARSVAVKPSVLLLDEPTSALDPISTGKVEELVHELKRD
                     YTIAIVTHNMQQAARVSDYTAYMYLGELIEFGSTEELFFKPKRKETEDYITGRFG"
     gene            complement(19483..20349)
                     /gene="pstA"
                     /locus_tag="PKV30_00955"
     CDS             complement(19483..20349)
                     /gene="pstA"
                     /locus_tag="PKV30_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRL28819.1"
                     /translation="MATIKLESALYKKRKSANAIGLALSMAAMALGLVVLLWILFVLL
                     SKGLAAVDWNMFTRDTPAPGSEGGGLRNAIVGSLMMLGLTVLVSTPVGILAGIYLAEY
                     GDESKTAELTRFVTDIMLSAPSIVLGLFVYAIVVAPMGHFSGFAGSLALSLIAIPVVV
                     RTTENMLRLVPGSLREAAAALGAPRWKVSLMITLRAAKSGVITGLLLAIARVSGETAP
                     LLFTALNNQFFSTNMSQPMANLPVVIFQFAMSPYDNWIRLAWGGALLITLTVLIINIV
                     ARVFFREKSAGH"
     gene            complement(20351..21352)
                     /gene="pstC"
                     /locus_tag="PKV30_00960"
     CDS             complement(20351..21352)
                     /gene="pstC"
                     /locus_tag="PKV30_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRL28820.1"
                     /translation="MTSINNLPHSPDKATSLIAPSMVAIARRQRWQDVLFHRGTQAFS
                     LLVLAALLGIIVSLFLNAWPTFKAFGLKFLWTAEWDIINEQFGAAIAVVGTVLSASIA
                     LFIAVPLAFGIALFLTENCPTWLRRPLGTAIELLAAVPSIIYGMFGLFVFAPIFADFM
                     QMPLQKVLGGMPLVGWLFGGAVNGMGILAAGIVLAFMILPYIAAVMRDVFEIVPPILR
                     ESAYGLGCTTWEVVRRVVLPYTQKGVIGGVMLGLGRALGETMAVTFVIGNANRMPTSL
                     FSPGTSIASVLANEFGEAADLHLSTLFALGFLLFVITFVVLALAKIMILRAERAKGT"
     gene            complement(21458..22444)
                     /gene="pstS"
                     /locus_tag="PKV30_00965"
     CDS             complement(21458..22444)
                     /gene="pstS"
                     /locus_tag="PKV30_00965"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HRL28821.1"
                     /translation="MGAFVSTAALAADITGAGATFPYPIYAKWAEGYKAATGTGLNYQ
                     SIGSSGGIRQIRAKTVAFGATDAPVKGEELDKDGMVQFPAIIGGTVPVINLDGFKPGE
                     LRITGPVLADMFLGNIAKWNDPKVAALNPGKNLPDQVITVVHRADGSGTTFNWTDYLS
                     TVSKDFADKVGKGAAVKWPAPTSVGGKGNEGVAANVNRIKGSVGYVEYAYVKKNKMNF
                     MQLQNKAGKYVSPDDEAFASAAAGVDWFSVPGMGVSMVDAKGDKSWPVSTASFILMYK
                     QPTDKAQSAEVLKFFDWAFKNGKQMAAELDYVALPDSLTNDIRSKVWSQIAK"
BASE COUNT         3688 a         7576 c         7556 g         3638 t
ORIGIN      
        1 ctgggcccgc gcgagaccaa ggccgccatc gccgccgcca accaagcttg gcccgcgtgg
       61 cgcgaactca ccgccaagca gcgccacgcc atcctgatga agtggttcga ccttttgatg
      121 gcgaatcagg acgacctggg ccgcctgatg accgccgagc aaggcaagcc gctgcctgaa
      181 gccaagggcg aagtcgccta cggcgccagc tttgtacagt ggtttgccga agaggccaag
      241 cgcgccggcg gcgagacgct gcccacgtcc gacccaacca agcgcctgat ggtgctgcgc
      301 cagccgattg gcgtgtgcgc ggcgatcacg ccgtggaact ttccgctcgc catgatcacg
      361 cgcaaggtcg cgcccgcgct ggcggcgggc tgcccggtca tcatcaagcc ggccgagctg
      421 acgccgctca ccgcgctggc cgcggccgag ctggcggtgc gcgcgggcgt gccggcgggc
      481 gtcatcaacg tgatcaccac caccgattcc agcgccgtcg gcaaggtgct gtgcgaaagc
      541 gaggtggtgc gccacctgtc gttcaccggc agcaccgaag tgggccgcat tttgatggcg
      601 cagtgcgcgc ccaccatcaa gaagctggcg ctcgagctgg gcggcaacgc gcccttcatc
      661 gtgtttgacg atgccgacat tgatgcggcg gtggaaggcg ccatcgccag caaataccgc
      721 aacgccggcc agacctgcgt gtgcgccaac cggctgtacg cgcaagacgg catctacgac
      781 aagttcgtca agaagctggc cgccaaggtg gccaagatga aggtcggcaa cggctttgac
      841 gacggcgtgg taattggccc gctgatcgag gacgcggcgg tgaacaaggt cgccaggcac
      901 gtcgccgacg ccgtgaacaa aggcggcaag gtgctgacgg gtggcgagaa gatgaagggc
      961 cagttctttg agcccaccgt gatctcgggc gccacggccg acatgctggt cgccaccgaa
     1021 gaaaccttcg gcccgctggc gccggtgttc cgcttcaaaa ccgagcaaga agccatcgac
     1081 gccgccaacg ccaccatctt tggcctggcc agctacttct acgcgcgcga catcggccgc
     1141 atcacgcgcg tgggcgaggc gctggaatac ggcatcgtcg gcgtcaacac cggcatcatc
     1201 tcggtcgagc aggcgccgtt tggcggcgtc aagcaatcgg ggttggggcg cgaaggctcg
     1261 cgccatgggc tggatgagta tctggagatg aagtacctgt gcgtcgggat ttgaccccac
     1321 ctgaggcgct gcgcgccgtc cccccagggg gactccgcca gcggccgggc caagcccgct
     1381 ccgcggcagc cccgcatggc ctgctccgcg gccattggct tttacgatcc acgcgccggg
     1441 gcggatggct cggtgcctgc ctgcttgccc tggccgcgcc gctggcacag gccgaagcgc
     1501 tgtggctgga cgatgccggc cgcccgcgcg ccgcgacgcg cgaggcggtg cgctggctga
     1561 ccgacgccga gcagcaaggc ctgcgcccgc aggattacga cgccagccgc ctcgcgtccg
     1621 ccgtggccga tgccgaacgc gccgcgccct cgcccgatgc ggccgcgcag ctcgacgaat
     1681 cgctcacccg ccaggtgctg gcctacctga gcgatctgcg ccacggccgc gtcgatccgg
     1741 cccgcatcca ggcgcgctac gacagcgcca cagcacccgc gcccaatctg cccgccgtgc
     1801 tgcgcgaagc ggttgcgcaa ggccagctgc aagacgccca gcgcgccgcc ttgccgccct
     1861 ggccgcagta cgccgatctg caaaaggcct tggtgcatta ccgcagcctg gccgatcacc
     1921 ccgcttgggg caaagcgctg cctgccctgc ccggcggcaa gctgcaggcc ggccagcgtt
     1981 gggcaggact ggccacgctg gccgagcggc tgcaggcgct gggcgatctg cccgaggcgc
     2041 ctgccaccgc cgtcaccgcg tacgacgcaa cgctgcaaaa agcggtgcaa tcttttcagt
     2101 cccggcatgc gctgccggcc gatggcgtca tcggaaagag cacgctcgac gcgctggccg
     2161 tgccacccgc cacccgcgcg caacagatcg cgctggccat ggaacgcatg cgcctgacgc
     2221 cgctgccggc ggcgccgcga tttgtcaccg tcaacgtgcc cgagttcacg ctgcgcgcgt
     2281 tccgaaccga cgccggcagc gtgcgcgagg atctgcagat gcgcgtcatc gtcggcaagg
     2341 cgctcgacac gcgcacgccg ctgttcgacg aagacatgct gtggatcgag ttcagccctt
     2401 actggaacat cccgccctcc atcgcacgca aggagacggt gcccacgctg cgccgcaacc
     2461 ccggctacct ggccgcgcag ggcatggaat tcgtaagcgc cagcggcgtc agcaccgacg
     2521 tcacgccgca gaatctggac gccgtgctgg ccggccaaat gcgcatccgc cagcgccccg
     2581 ggccgcgcaa cgcgctgggc gacatcaagt tcatgctgcc caacaaccag aacatctacc
     2641 tgcaccacac gccctcgccc ggcctgttca gccgcacgcg ccgcgacttc agccacggtt
     2701 gcgtgcgcat cgaagagccg gtggcgctgg cgcaatgggt gctgcacgac cagcccgaat
     2761 ggaccgaggc gcgcatccgc caatccatgg gccgcccgcg ccccgtcacc gccaagctgc
     2821 tggagccggt gcccgtgctg atgctgtacc agaccgctgt cgtcgacggc ggcaagctgc
     2881 acttcgtgcc cgacgtgtac gggcaagacg cggtgctgga aaaagccctc aagcaacccc
     2941 gccccgcgcc cgccgccacc gtgacacccg tcacgcgcgt gcccgccgcg ccacagccag
     3001 tgtccgcgcg cgctggcgca tcggattcga tagcggcaca atgagtcgcc acgccgaccg
     3061 cccacccatt tgtaacatca ccaccatgca gaacccgcac cgccgcttgt tcctgggccg
     3121 cagcttcagc gccgccaagg tggccgccgc cggcgtgctg ctgcccggcg ccgcgcgcgc
     3181 tttctcgccc gccagcacgg ccggcgcgcg ccgcctgtcg ttcaaccacc tgcacaccaa
     3241 cgagcgcacg gcgctggtct atgccgtggg cggcgacttt gtgccgcgcg cgctcgacca
     3301 cctgaaccac tttctgcgcg accactacac gcaggaagtc ggccagatgg accccgagct
     3361 gtacaacctg ctgcacgccg tgcggcgcga gctgggcacc gagctgccct tcgacgtcat
     3421 ctccggctac cgcagcccgc acaccaacga aaccctgcgc accacgcgcg gcggcggcgt
     3481 ggccaagcgc agcctgcaca tggacggcaa ggccatcgac gtgcgcctgc ccggcgtggc
     3541 gttgaccgat ttgcgcgatg cggccctgtc gctcaaggcc ggcggcgtcg gcttttacga
     3601 gagcgacaac ttcgtgcaca tcgacaccgg gcgcgtgcgc agctggggtt gaagcggcgc
     3661 cttcacatcg cggcgaaact ctgcttttga tagctgctcg cgctttctgg acaagcgcca
     3721 gcgccctttt tctcttaaat atcaaccgtg ctcgagccgg cgcagcacgc cccgcgccgc
     3781 ccgcacgccg ctggccaggc aggccgtgag caggtagccg ccggtcggcg cctcccagtc
     3841 cagcatctcg ccggcgcaga acacgccggg cgcggtgcgc agttccaggt cttccgtcaa
     3901 ggccgacaga cgcacgccgc cggccgtgct gatcgcctcg gccaccgggc gcggcgcgcg
     3961 cagcgtgatc ggcagcgcct gaatcgcttg cgccagcgcg gccgcgtcct tcaattgctc
     4021 ggccgtcagc aactcgtgca gcagcgcggt gtgcaccggc gccaggccga gccggctttt
     4081 caactgtgac gacaggctgc ggctgccacg cggatgcgcc acggcggcgt gcacatccgc
     4141 tgctgtgcgg cccggcaaca ggttgagcgt gaaggtggcg ctgccggcgc gcgcgatctc
     4201 gtcgcgcagc gcggcgctgg cggcgtagat caggctgcct tcgacgcccg tggccgtcag
     4261 cacaaactcg ccgcgccgct cgaagcgctg gcccgcgtga tttgtgaaag tgatcgacac
     4321 gttcttcagc ggctggccgg caaagcgctc gctgaaaaat ggcgtccagc cgtcgcctgc
     4381 gcggcctgcc gcgtcgaagc cgcagttggc gggttgcagc ggcgccacgt cggcgcccat
     4441 ctgctgcagc cacggcatcc aggtgccgtc cgaacccagt cgcggccagc tcgcgccgcc
     4501 cagcgccagc acggcgacgc gtggctgcac attgacctcg cccaccggcg attcaaagca
     4561 aaaaaggccg ccagcgcttg atggacttgc gctggcagct actgaattca tagttttctc
     4621 agcttgccag cccacccacc gatgccgcat gtgaaagcgc actggcgcgc cgccggccga
     4681 cgggtggcgc agccgctgca gccacgcgcg cagcaacggc gcggccttca tctcggccgg
     4741 aaacacgcgg cccgacgtgc cgacaaaggt ttcgacgccc agccccgccg cccattcgcg
     4801 cgtggcgtcg ggcccgaagt cgcccagcca attcgccacg gtgtcggtct gcgcaccgta
     4861 gcgaccgatg aaggcgggca gcggctcgga atgcgtcagg ttgagcccgc ccttgcccgc
     4921 cagcaggaac ttgcggccga cggagggcat ggcgtcgaac acatccaccg cgacgcctgc
     4981 gccggagagc acctcggccg ccatcaggcc ggcggggccg gcgccgatga tgaggacctg
     5041 gggggtgggg ggtgtcatgt tctttttttc ggctgtgatg ggaggggcga tggtacgggt
     5101 cgcggtgggt gaagcgctgt tggccgggag ttcgccccgg cgggcgacct acttttcatg
     5161 ctcgcacaag aaaagtaggc aaaagaagtg cgccccaccc cgcccgcgag caccgcaggt
     5221 tgcccgcagc gaagcggagg gacgcagcca gtggggccgc cttttctttg gttactttct
     5281 tttggcggag caaaagaaag tgactgcgcc gccgggcgca catcccggcc aacagcgcat
     5341 ccaacccaaa agctcaacca accgatacaa cccttccatc cgcgctcaaa aacgccgcca
     5401 ccaccgtctg gcccgggtgg atcaacgaca ccgcttctcg atatcggccg agttgctcca
     5461 ccagcaacgc atcacgctcc ggccgcgcgg cgcttttgta gtcgagcacc caccacgcct
     5521 catccccatg cgcaccggcg cggcggcgca ccagccggtc gatgcgcagg cgctggcctt
     5581 ggtgggtgag ttcgatctcg ttgaaggcct ccagcacttc accgccagcc caggcccaag
     5641 cggcatcacc cgtgaggatg cggcgtgcca gggtttcggc acgggcggcc tgatcggcgt
     5701 cggcgccaaa gcggcggcgc gcttgcgcca cacgctcggg cagccagccc tgcggggtgt
     5761 cggcggcgtg ctccagcagc cagtgcatgg cttcgccaac gcgcgaggtg tcagtgggtt
     5821 cggctttggc aacttcactt tcgatagctt cttgcgcttg ctggacaagc gctggcggca
     5881 gttcgagcat tgcaaaactg tcttcatcgc cggcgggcgc cacggcatcc tgcggcacgg
     5941 gcagcggctc ggccagggct tcgatgcgct gccaccagct gctggccggc tggctgtacg
     6001 gcgtgacgcc cgagagcacc agccgcccgc gcgcacgggt catggcgacg tacagggcgt
     6061 tgagttcttc gcgcgcttgc gcggcggctt cttgcgcggc caggtcggct acaccgggcg
     6121 ggggggcgtc ctcgctggcc agaaagacca ggcgcgtggg cgcgggctgc tcgcccggcc
     6181 agtcgatcag cgtggtgggg ccgccgccgc gcggggcggc gctggcggcg tcgagcagca
     6241 gcacttcgtc ggcctccagc cctttggcgc cgtgcacggt gagcagctgc acggcctgcg
     6301 gcgccgatgg gcgcggcgcg ggcaggcgct ggcggcgcag tgcgcgcagc cactggtagg
     6361 cggtcaagaa gcgaccgccc tgcacgcgca gcgcggcggc cagcagcgcg cgcagttgcg
     6421 ccaacacctg cgcgcgttcg ggcgccggcg cggcagcggc aaagcgggcc agcacgtcgc
     6481 ggtggctgta gatggcttgc aatgcgtcgt gcggtggcag ggtgagcagc caatggcggt
     6541 attgagtcaa atcggccgcc agcgcatgtg tatccagcgt gggcagctct gtttttggga
     6601 gtacttcgag ccaggcgggg ggggtgccgc gaggcggcag gggcgtgtcg ggcgcgggct
     6661 gcccctcacc cctgccttct ccccaggggg gcgagggagt agaagcgcgc acggccaacg
     6721 cgatgcgcac caggtcgtcg tcggtcaggt caaacagcgg gcttttcagc gcccgcgcga
     6781 gcgccaagtc gtgccccggc gagacgagcg catcgaccag agccagcacg tcttgcaccg
     6841 ccgcttgctc gcccagcacc tgctgctcag gctgttcgca ggcgatgccc agctggcgca
     6901 gctcggcctg cagttcgccc agcggcaggc gcttgcgggc cagcaccatc aggcgatcag
     6961 gcggcacgcc agcggcgatg cggctggcga tccagcgggc ggcctggcgc gattcgaggg
     7021 tgcgcaggct gtcttcgagc gctacgcgcg cggtggtcag gctgtcgcgc caaacgggtt
     7081 cgtcgccctc gcctgtcgcc gcgcgatcgg ggcgcgggat gagcggcagc gcgccgatgt
     7141 ggccgcgcgc ggtggattcg gtgctgtggg cgcgatagcc gctgaactcg tcggcggcct
     7201 gcgcggccag catcacggtg ttgacggcgc ccagcacttc gggcgcgttg cggtgcgtgt
     7261 ggtcgcaggc caggcggtcg ccgtccagcg cgttcacgat gaagtcctgc gcggcgatga
     7321 agacctgcgg gtcggcacgc cgaaagcggt aaatgctttg cttggggtcg ccgacgatga
     7381 acacgctggg cgcgctggtg ccgccaccgg cgccggcata ggacgacagc cacgcgtgca
     7441 gcgcctgcca ttgcagcggg ttggtgtctt gaaactcgtc gatcagcagg tggcgggtgc
     7501 gcgcgtcgag ccgctcttgc acccagccgc tgaggaacgg gtcggacagc agggtgagcg
     7561 cggcgcgctc gacatcgctc atgtcgaccc agccgcgctc gcgcttgagt tcggcaaacg
     7621 attcgatcag cgggcgcgtg aggcgcgtca tgcgcaggtg gtattggcgc gcggcgtgct
     7681 gcgcttcggc ggccagcacg gcttcgacct cgttttgcgc ggcgcgcacg tcgcccgggg
     7741 tgtgcttgct gaaggcgcgt ggcgtgccgt ttttggtgaa cagcgcggcg accacgccgt
     7801 cccaatcttc cgcttcgaga gcggccagca actcggcgcc cttggcggcg taggtgcgcg
     7861 ccagcggggc cagcgcgcgg gcagcgtctt gcagcaaggc gcggccggcg gcgcgctgca
     7921 gcagccagtc ggcgggctgg gcgacgccgg ccaggcgcgg gtagagcacg tcgaaaggtt
     7981 caacagcgtc atcgacgtgg ccggcggcat cggccagcag aaattcaacg cggcgggcca
     8041 gcgcgccttc cagcgcggcg tgggcgcggt ggcggcccag cgtggcgacc agggcggcgt
     8101 agtcggcgct cagatccgca tcgcccgcca cgcggcgcag gtagcggcgc cagacctcgg
     8161 ccacggcgtc ggcatcgtct tcgagcagct cgtaggtgga gggcaaaccc agctgctgca
     8221 acaccgacag cggcgcgctg cgcagcaagg cggcaaacca gctgtgaaag gtgcggatct
     8281 gtaccggtcg accctgggcc agcagcgatt ggtataaatt ttgtagctgc tcgcgctgac
     8341 tgggcaagcg ctggggctcg attcctcttg caagcagctc ggcatccagg cgctgctgcc
     8401 aggcttcggg cgtctcggcg gcgcgcggcg cggcgaagtc ggccagccac tgctgcagcc
     8461 gctcgcgcat ctcgcccgcg gccttcttgg tgaaggtgat ggccagaatc tcttgcggcg
     8521 cggcgccacc cagcagcgcg cgcaggatgc gcgacaccag catccacgtc ttgcccgcgc
     8581 cggcgcaggc ctccaccgcc acgctgcggc gcgggtcgca ggcaatggcg tagaaggcgg
     8641 cgcggctgca aggggcgccg ttgtgttcgt aggcgggggt ggtcatggcg ctgctccggc
     8701 gcagacacct gcactcgggc tgcgcagcaa gcccttcacc aggccgcgga gcaggccgtg
     8761 ccagaacgcc gtggacgggg tctccccgtc caccagcgtt gtccccctcg gggggagggc
     8821 gcgcgtagcg cgactcaggg gggtggtcat gttttccaga aatccttgcg gcacagcccg
     8881 cgcgcgtcgc accagccgca cacgctgcct tcgcccagcg cgggcagcgg ctcgccggcg
     8941 gcaatgcggg ccaggtcgta tgccatgcct tcgtacagct gcgcggccag catgggcagc
     9001 tcgggcaact cgtgcaggct ggggacttcg cgttcggcca ggtttaggta ggcggcgcgc
     9061 ggggcgtcgg cgccggacag cagggcgtag aacggcaact gcgtgtcttc gttgccggcc
     9121 ttgatgcgat cgcgcgtgcg ggcgagcggc tcggtcttgt agtcgatgag cagcgtggcg
     9181 ccgtccgtca cctcgtcgat gcggtcgatc tggcctttca ggcgcagcgg gccgcgttgc
     9241 gtgtcgatgc tttcttcggc cagcttgaat tgggcgccgc cagcttcgtg cttggccagc
     9301 cattgcaggt aggcatcgcg cagcgtgggc caggcgacgg cgaagggcat gaattcgccc
     9361 ggctccaggc cggggccgag cgcctcggtg gcggcttcgg cggcggcgtc gatcagggtc
     9421 agccggtcgg catcgggcgc gacacgcagc gcttcgtgaa agccgcgcag cgtgtcgtgc
     9481 agccagttgc cccaatcgcg cttgtcgatg tcgacgtcca gctcgccctc ttcggccagg
     9541 cccaactggc gcagcgcgaa gaagcggtag gggcaggcgc gcagcatctc gtagccgctg
     9601 gcggacagcg gctgggtggg cagtgcgtcg ccgcgcggct gcgggcgttc ggtggggcga
     9661 atttgaatga aacgggcctc tcgtccttct gcacccagcg cagccagctc ctgaagttgt
     9721 agcgattgca ccaggggcga aggcagcagc ggctcgccgc tgtcgtcagt gttgcgccac
     9781 agcacgtcga cctgcggcac gcgcagcgcc agcgcccagg cggcggcttg cgcgtcgcgc
     9841 aaatcctcgc gcgtgggcag gtgcagggcc tggcgctggg cggcgctcca cgggccaggc
     9901 ggctcgggcg cggcgggcag gcgctgttcg tcggcgccgg gcagcaccag cgaggcaaag
     9961 gggcggccca gcaactgcgt cagcggcagc accacgactt gcgcggcggc cgggtgcggc
    10021 gggcggaagc tggcggcttc cagcgcctcg ccgacccaac gggtgaattc ggccaggccc
    10081 atgcggcgct gggcggcggg ccagtcgcgc cagtcggcca gggcttcgtc actcaagccg
    10141 agggcgtcaa tgacggcgtt gccggccacg tcggccgcca gcagcggcca caggccgcag
    10201 ccttgcagca gcgtgcgcag ggcgggcagc cagtcggcca gtgggcgcgc ggccctcatg
    10261 gggacgcgca gcgattcgat gtgctgcgtg agcggctgat cgcccgtgag cagcgcggcc
    10321 tgtggccagc cgcgcacggc gtcgcggcgc aggcggcgtt ccagcgcgcg ctgctgccgc
    10381 gcatcgaagg cgggggcgag cttgagccag tcgagcacct cgtctgtgga ggcgcgcggc
    10441 gcgcacgcgt gcagcgccgc catcagttgc gcggcggcgt gggtggtgga aagcttccag
    10501 ccggtctcgt cgcgcaactg ggcaccgcgc tcgatcagca gcgcgttgat gcggcgcgtg
    10561 agcaggcggt cgccggcgat cagcgccacc ggcacgcggc ccacctcgat ttggcgcagc
    10621 acgcaggcgg cggcgcgctc ggcttcgtct tcggcatcgg cgcaggtgtg cagggcgatg
    10681 tggcccagcg ctgcatcgga ctcggggtgc agtagcacgg ctttctcaaa gtagtgctcg
    10741 gccagcgtct cggtcagggg gtcgggcagc aggccgggca cgatcagcag cgcgtccagc
    10801 gcatcggcca cgcgaggtgc aaacaaaacg tcggtggcgt agtccgagcc acccgcccaa
    10861 gcgatggcga tgcggcccac ggcggcttcc agcgccagcg ggccatcgcc cacgggcggc
    10921 agcgcgccac gcgcctgctc ggcccagtcg gggcgcagcg cgggcggcag gctggcggcg
    10981 atctggccca gctgcgtggc ctgctcgacc agcgggccgg ccagcagcgc gcgctgcgcc
    11041 gccaggcccg cgccctcgag cagcgcggcg gcggtgagca ggtcgcggcc gtggtcaaag
    11101 ctcagatcgt gcgggccggg cgtgaaccag ccagcgcgcg tggcccagtt gcgggtggtc
    11161 tcaaaacgcg gcgcaaacgc atcgggaaac tgcgccgccc acagccgcgc ggccagcggc
    11221 atcagctgcg cgtagggcag cagcaccacg ctgcgcgcag ggtgcgcggc cagctggcgc
    11281 aactcggcat cgatgcgcgc gagcaagccc tgccaagcat tcaatacacc gctggtcgtg
    11341 gtggcgccat cgttttcagc tatcgccgtc atagcatcaa cggacgcctg tggcgactgt
    11401 ggggaaaagg tgtttctgtc acaatgaccg gcactttatc ttctcaacgc ccgcctttag
    11461 ccgggccttc acatcatgcc caacgaattg atcaagcatg tgagcgacag ctcttttgaa
    11521 accgacgtcc tgcaatccga caagcccgtg ctggtcgatt tctgggccga atggtgcggc
    11581 ccctgcaagg ccatcgcgcc aacgctggac gagctggcca ccgcctacga cggcaagctg
    11641 cagatcgcca aggtcaacgt tgacgagaac cgcgcggtgc ccgccaagtt cggcattcgc
    11701 ggcatcccga cgctgatggt gttcaagaac ggccagttgt cggccaccaa agtcggcgcg
    11761 ttgaccaagg cgcagctgtc gcagttcatc gaccagcaac tggcctgaac gcttccccaa
    11821 actggtccaa gcgctggttc tggccagttt tcttttgctg tcataatctc tcttcgaaag
    11881 ccggtgcaca ggcaagcacc ggccggcgcc ctgacccaca gcgcgcccgc accgaagccc
    11941 atctccaggg cttcgagacc tcccccaaaa tcttctctct atcgccctac cgattggccg
    12001 atcccgatgc cggatcgccg tccactgcaa gacgatcgcc atgcacctga acgaactcaa
    12061 agccctgcac gtctccgaac tgctcaagca ggccgaaacg ctggaaatcg acaacgccgg
    12121 ccgcatgcgc aagcaggagc tgatgtttgc gctgatcaag aagcgcgcca aggccggcga
    12181 gcaggtgttt gccgatggcg tgctggaaat attgcccgac ggcttcggct ttttgcgcag
    12241 ccccgacagc agctacacgg ccagcaccga cgacatctac atcagcccca gccaggtgcg
    12301 ccgcttcaac ctgcacaccg gcgacatgat cgagggcgag gtgcgcatcc ccaaggacgg
    12361 cgagcgctac tttgcgttga ccaagctcga catggtcaac ggcagcccgg ccgagcagaa
    12421 caagcacaag gtgatgttcg agaacctgac gccgctgttc ccgcgcgagc agatgcgcct
    12481 ggagcgcgat gccttcaaag gcgaggagaa catcaccggc cgcatcatcg acgtcatcgc
    12541 gcccatcggc aaaggccagc gcgcgctgat cgtggcgccg cccaagagcg gcaagacggt
    12601 gatgatgcag accatcgccc acgccatcag cgccaactac cccgaagcgc acatgatggt
    12661 gctgctggtc gatgaacgcc ctgaagaagt gaccgacatg cagcgctcgg tcaagggcga
    12721 ggtgatcgcc tccaccttcg acgagcccgc cgcacgccac gtgcacgtgg ccgagatggt
    12781 gatcgagcgc gccaagcgcc tggtcgagtt gaagaaggac gtggtgatcc tgctcgactc
    12841 catcacccgc ctggcgcgcg cctacaacaa cgtcgtgccc tcgtcgggca aggtgctgac
    12901 gggcggcgtg gacgccagcg ccctgcaccg ccccaagcgc tttctgggcg ccgcgcgcaa
    12961 tgtcgaggaa ggcggcagcc tgaccatcat cgccaccgcg ctgatcgaca ccggcagccg
    13021 catggacgag gtgatctttg aagaattcaa gggcaccggc aactcggaaa tccacctgga
    13081 tcgccgcctg tacgaaaagc gcgtgttccc gtcgatccag ctcaaccgct caggcacgcg
    13141 ccgtgaagaa ttgctgctgg cgcccgaggt gctgcaaaaa acccgcatcc tgcgccagtt
    13201 catgtacaac atggacgaga tcgaggcgat ggagatggtc atcaagagca tgaaggcgac
    13261 caagaccaac gtcgagttct tcgacatgat gcggagaggg ggctgacccc ctgagccgct
    13321 gacgcggctt cccccctttg cttcgcaggg gggacatcgc cgatggccgg tacaagcccg
    13381 tccatggcga tcgctggcat gcccggctcc gcggtcaccg gattgtcttt tgggcaatcc
    13441 atttggtata atgcgcggtt tcgtggaaag tacgccggat gggccggcgc ggctgccgca
    13501 gcgaccctca aggaaaacac atcatgcgtg aaggcattca ccccgactac cgcgaagtgc
    13561 tgttcgtgga cctgtccaac ggcttcaagt ttgtgacgcg ttcgtgcgtc aacacgaaag
    13621 aaaaagagac tttcgaaggc aaggaatacc cgctgtacaa gctggacacc tccagcgagt
    13681 cgcatccctt ctacaccggc acgcagaagt cggtcgacaa catgggtggc cgggtcgaga
    13741 agttccgcaa ccgcttcggc aaggtcgcac gctaacaggc tttccgcagg ctccgccacg
    13801 ccggcgccgc gcacaagact ggaacaaggc agcacggttt cgtgctgctt ttttttcgcg
    13861 catcgccttc ccgcagaatg gcttgtcgtc aacctggcct gtagccttga accccaaacc
    13921 cagccccgcc atcgtcacgc aggacgcggc ccggcgcctg ccgcgctggg ccttgctgtt
    13981 gttgtgcgtc gcgtacgtca tcccgggcta cgtgggccgc gagccgtgga agaacgccga
    14041 catcgccagc ttcggctaca tgctggcgct gctggacgcc ggcagttggc gcgaatggct
    14101 gcagccttca atgatgggcc tggtgcccga caacggcgcg ctgctgcctt actggctggg
    14161 cgcctgggcc atgcaggcgc tgatgccgct gggcctggcg cccgaaatcg ccgcccgcgt
    14221 gccatatctg ctgatgctgg cgggcacgct ggtggccacc tggtacgccg tgtaccacct
    14281 ggcgcgcgac cccagcgcgc agccggtggc gtttgcgttt ggtggcgagg ccgagccggt
    14341 ggattacgcg cgcgccctgg ccgacggcag cctgctggcg ctggtcgcca cgctggggct
    14401 ggcgcagttc tcgcacgaaa ccacgccggc gctggcacaa ctgtttttcg gcacgctggt
    14461 gttctacggg ctggcggcgc tgccgacccg gccgctggcc ccgttcatcg cgctcctcgt
    14521 tggcttgccg gggctgacgc tgtcgggcgc acccgccatg gcgatgattt acgccggctt
    14581 ggggctgctg gcgctggccc tgccaggtgc ggctcctgcg catggcgccg cgcgtttgcg
    14641 cgccttgctg cagttgctgg cgctggtggg cttgtgtctg gcactggcgc tggcgcttga
    14701 tctgttccgc tggcggatcg cgccctggcg caccagcggg gccgaatggt cgtcgctcgc
    14761 ccgcctgttt ctgtggttca cctggccggc ctggcccttc gtgttgtgga ccttgtggcg
    14821 ctggcgccgc cagttgctgt cgctgcgcca gcagcgccac ctgggcctgc cgctggccat
    14881 cgccagcgtg ccgctgctgg gcacgctgct cacgctggcc ggcgaccgcg cgctgttgct
    14941 ggcgctgccc gcgctggccg cattggcggc actggcactg cccacgttca gccgcagcgt
    15001 gtcggcgttg atcgactggt tcacggtgct gtttttcacc ggctggacgc tggtgatctg
    15061 ggtcgtctgg gtggccatgg agaccggcgt gccggccaag ccggcggcca acgtggcgcg
    15121 gctggcgccc ggctttgatc ctgtgttcca gtggccagct ttcgtggccg cgttgctggg
    15181 cacgctggcc tggttcgcgc tggcgcgctg gcgcaccggc cgccatcgca ccgcgttgtg
    15241 gaaaagcatg gtgttgccgg cggccggcac cgcctcgtgc tggttgctgc tgatgaccct
    15301 gtggctgccg gcgctggact atggccgcag ctacgcgccg cagatgcgtg aagtgcgcgc
    15361 gctgatcggt gatgccagct gcgtcgaagt gcatggcttg accgaggcgc aggtggccgc
    15421 cgtgcgcttt catggcggct ggttgaccgt gccggcgcgg ggcccggtgg cctgcccgtg
    15481 gctgctggtg gatgtggatg cgcagtcgag cctgacggcg tcggtgtcga tgaccgactg
    15541 gcgcgaggtc ggtcaggtgc gccggccgac cgaccgcaac gaaacccttt acgtgctgag
    15601 gcgcgtcgga cggccttgag cgtcagctgg cgtggtggct gacgccatcg gcgagcccaa
    15661 gtcacgcaac gcctccgttg ggcgacttgc ttaaaagcta tgatttttgt agctgttccc
    15721 gcttgtctgg caagcgccag gccgtgattt gatgcagaaa gggcagttgc agtgctgcgt
    15781 gtgcccgatc aagcctctgc gttgggcgcc acagaacccg gcacgagcgc agccgtcgaa
    15841 gccgccctca cccgctgcgg cggaaattca agcgcaaaga tcgagccgtg acccagctca
    15901 ctgcgaatct gcagctcgcc cccgtggcgc tgcgccacgt gcttggtgat ggccaggccg
    15961 agcccggtgc cgcccgattc gcgcgagcgg ctgcgatcaa cgcggtaaaa gcgctcgccc
    16021 agccgcggca ggtgttgcgg cgcaatgccc ggcccgctgt cggtgacgac caggcgcgcg
    16081 ccgccgtcgg gcaggcgctg ccactgcact tcgatcagac cgccgcccgg cgtgtagcgc
    16141 acggcgttgt tggtcaggtt ggagaccgca ctgcgcaact cgttcgccga gccggtgaga
    16201 tcaaacatgg gcgctgcgcc aaatcgcaag gtctgcggcc gctcgtcggc cgggtgcatc
    16261 agattcgaca ggccgcgcgc ctcggcttcg cactgcgtca tcagcgcgcg caggtcgatg
    16321 gcctcgcccg cgccaggcag cggactgccc tccaggcgcg agagcgtcag caagtcgttg
    16381 accagcgtct gcatgcggtc ggactgcgtg gccatcatgt ccaggtactt ggcgcgctct
    16441 tcttccgaca gcggcaggct ttgcaaggtc tcgacaaagc cggccagcac ggtgagcgga
    16501 gtgcggatct cgtgcgacac attggcgacg aaatcacgcc gcatggcatc ggcctgcgcc
    16561 agcgccgtga tgtcgcgcga cagcatcagc tggtgcccgt tgccgtaggg gtgcaactgc
    16621 accgacagct tgaccgggtg cgccggcgtg ttgtggcggc cgatcagctt gacctcgcgc
    16681 tcatggttct tgctggcgta gtaggcgctg aaggccggct cgcgcaccaa gttgcccacg
    16741 tgctgcaaca gatcgcgctg ggggtcgatg ccgaagtgat cggccgcgat gtcgttgcac
    16801 cattcgatgc ggccgttcgg gtccagcaac aacacgccgt tgggcgacgc ctggatggcc
    16861 gccagaaaat cgttcaggcg ctggctgctg agctgcgcct gctgctgctc ggcacgcagc
    16921 gcgcgcgccg tgcgataggc ggcctcgccc caggcaccgc gcagcgcggg cacggtggac
    16981 gcattcgtct tgctgatcca gatcagaaag cggcgcgcac gccaggcgtc gatgccccag
    17041 gtcagcgctg cggccagcac cacgccggca agcgcgcccg gccagccgag ccaccagccg
    17101 cccagcaggc ccagcgccag atgtgccagc gcaacaagaa aaagccgaag cgccaagcgg
    17161 cccctcaggt ggtcatgaag aagtcgccgc ggccggcgcg ccgtgctgca actgcgccat
    17221 cggctgcgcc gtcaggcgat accctgcgcc gcgcacggtc tcgatgagca caccggcaac
    17281 gcccagtgct tcgcgcaggc gtttgacgtg cacatccacc gtgcgctcct cgatgaagac
    17341 atgatcgccc cacaccttgt cgagcagctg cccgcggctg tgcacgcgct cggcgttctt
    17401 catgaagtag tgcagcagct tgaactcggt cgggcccatc ttcagcatct ggccatcgta
    17461 ggacacccgg tgtgtcgatg cgtccagcag cagcgcgccg atggcgaccg agccgcccgc
    17521 ctgctcgggc gagcgccggc gcagcacggc gcgtatgcgc gccagcattt ccttggtcga
    17581 gaaaggcttg gtgatgtagt cgtcggcgcc agcgtccagg ccggcgacgc ggtcggcctc
    17641 gtcgccgcgc gcggtcagca tcaggatcgg cacgcccttg gtgcgcgcgt ccttgcgcca
    17701 tttcttggcc agcgacaagc cgctttcgcc cggcaacatc cagtcgagca ggatcacatc
    17761 gggcagcacg gcgtcgagtt cgcgctgtgc cgtttcgctg tccatcgccc aggtgggctg
    17821 gaagccgttg tgccgcaggt tgaccgcgat cagttcggca atgggtgcct cgtcctcgac
    17881 gatgagcact cggggcaggg ttctcatcgg gccacggact ccaggtcttc catcgtgccg
    17941 tggcgcacgt cggcgccctt gacgatgtag atgatgaatt cggcaatgtt cttggcgtgg
    18001 tcgccaatgc gctcgatggc cttggccagc gtcagcagat ccagcgcggc ggagatggtg
    18061 cgcgggtctt ccatcatgta ggtgatgagc gtgcgcacga agccgtcgaa ctcgcggtcc
    18121 acctggtcat cgtcgcggat gatggacacc gcgtcatcgg ccgacaggcg cgcaaaagca
    18181 tccagcgcgc ggcgcaactg ctgcgcggcc aggtcggccg catggcgcaa ctcgcccgtg
    18241 ggcagcatgc gcgccttgcc ggaatcgatg atgcgcttga ccatgcgcgc tatttttgcc
    18301 gcctcatccc ccacgcgctc caggttggcc gtggtcttgg agatggccag cagcaggcgc
    18361 aggtcgatcg ccgtgggctg acgccgggca atgatgttgg acagctcgcg gtcgatctcg
    18421 agctcaagcg cgttcacgcg ctgctcgtct ttttcaacct ggtcggccac ttcgatgctg
    18481 aattccgaca acgcatagat cgcctgccgg atctgcatct cgaccaggcc acccaactcc
    18541 agaacgttgg atgagacgcg attgagttcg ctgtcgaatt gactggacag atgcttctcg
    18601 tacatgaaac ttgtctcctg ttaaccgaaa cggccggtga tgtagtcctc ggtctccttg
    18661 cgcttgggct tgaagaacaa ctcttcggtc gagccgaact cgatcagctc gcccaggtac
    18721 atgtacgccg tgtagtcgct cacacgcgcc gcctgctgca tgttgtgcgt gacgatggcg
    18781 atggtgtagt cgcgcttgag ttcatgcacc agttcctcga ccttgccggt agaaatggga
    18841 tccagcgccg aggtcggctc atcgagcagc aataccgacg gcttgaccgc cacgctgcgc
    18901 gcgatgcaca aacgctgctg ctggccgccc gacagtgaca ggccgttctg gcccagtttg
    18961 tccttgactt cgttccacag cgcggccttg gacagtgccc attccacgcg gtcgtccatg
    19021 tcggccttgt tcaggctctc gtacaggcgc acgccgaagg caatgttttc gtagatcgtc
    19081 atcggaaacg gcgtcggctt ctgaaacacc atgccgacct gggcgcgcag caggttgacg
    19141 tcgacgctgg ggtcgagcac gttcttgcca tacagcagga tctcgcccac cgcgcgctgg
    19201 cccgggtaca ggctgtacat gcggttgagc gtgcgcagca gcgtcgactt gccgcagccc
    19261 gacgggccga tgaacgcggt cgccttgtgc tccgggatcg acaggttgac gttcttgagg
    19321 ccctgaaatc cattgccgta gaagaagtcg aggttcttga tttccagtgc caggcgctcc
    19381 tggtcaaaac tgacgccatt gccgcgaatg ctggcttgcg ggacgcgggt cgcaacttcc
    19441 atgatgaatc cttgttgaat tttgggcgat acgggcgggc gctcagtggc cggccgattt
    19501 ttcgcggaag aacacccgcg ccacgatgtt gatgatcagc accgtcagcg tgatcagcag
    19561 cgcgccgccc caggctaggc gaatccagtt gtcgtacggg ctcatggcga actggaagat
    19621 caccaccggc aggttggcca tgggctggct catgttggtg ctgaagaact ggttgttcag
    19681 cgcggtgaac agcagcggcg cggtttcgcc gctgacgcgc gcaatcgcca gcagcaggcc
    19741 ggtgatgacg ccgctcttgg ctgcgcgcag cgtgatcatg agcgacacct tccagcgcgg
    19801 cgcgcccagg gcggcggccg cctcgcgcag gctgccgggc accaggcgca gcatgttttc
    19861 ggtcgtgcgc acgacgaccg gaatcgcgat cagcgacagc gccagcgacc ccgcgaagcc
    19921 ggagaagtgc cccatcggcg ccaccacgat ggcatagacg aacagaccca gcacgatgga
    19981 gggcgccgac agcatgatgt cggtgacaaa acgcgtcagc tcggcggtct tgctctcgtc
    20041 gccgtactcc gccagataaa tgcccgccag gatgccgaca ggtgtggaca ccagtaccgt
    20101 aaggcccagc atcatcaggc tgccgacgat ggcgttgcgc aggccaccgc cttcggagcc
    20161 cggagccggc gtgtcgcgcg tgaacatgtt ccagtcgacc gctgcaaggc ccttgctgag
    20221 cagcacgaac aagatccaca agagcacaac aaggcccaac gccatggccg ccatcgaaag
    20281 tgcaagccca atggcgttgg cggacttgcg cttcttgtag agcgcgcttt ccagtttaat
    20341 cgtcgccatc tcaggtcccc ttggcgcgct cggcgcgcaa tatcatgatc ttggccagcg
    20401 ccagtacgac gaacgtgata acgaacagta gaaagcccag tgcaaacagc gtcgacagat
    20461 gcaagtcggc tgcctcgcca aactcgttgg cgagcaccga ggcaatcgat gtgccaggcg
    20521 agaaaagcga cgtcggcata cggttggcat taccgatcac aaaggtgacg gccatggtct
    20581 cgccaagggc gcggcccagc cccagcatga caccgccgat gacgcctttt tgcgtgtagg
    20641 gcagcaccac cctgcgcaca acctcccaag ttgtgcagcc cagaccgtag gcggattcac
    20701 gcagaatcgg cggcacgatc tcgaacacat cgcgcatcac ggcagcgatg tagggcagga
    20761 tcatgaatgc cagcacgatg ccagcggcca gaatccccat gccgttgacg gcaccaccaa
    20821 acagccagcc cacgagcggc atgccaccca gcactttctg cagaggcatt tgcatgaaat
    20881 cggcaaatat cggcgcgaac acgaacaggc cgaacatgcc atagatgatc gaaggcactg
    20941 ccgccagcag ttcaatggcc gtacccaggg gacgccgcag ccacgtcggg cagttttcag
    21001 tcagaaataa cgcgatgcca aacgctagag gcaccgcgat gaacaatgcg atgctggcac
    21061 tcaacaccgt gcccacgacc gcaatggcag caccaaactg ctcgttgatg atgtcccatt
    21121 cggctgtcca aagaaacttc aggccgaatg ccttgaaggt cggccatgcg ttcaggaaca
    21181 gggatacgat aatcccaagc agcgccgcca gcacgagcag cgagaaagcc tgcgtgccgc
    21241 gatgaaaaag cacgtcctgc caacgctggc ggcgcgcgat ggccaccatg ctgggggcga
    21301 tcaggctggt tgccttgtct ggcgaatgcg gcagattgtt gatggacgtc attcccacgc
    21361 tcatacagtc ctcatcacgc tcattatggt ttgtgcggct tgaaatcggc ccggcaggca
    21421 ctggctgcgc ctgccgggcc gcgctctgaa ccaggcgtta cttggcgatc tgcgaccaga
    21481 ccttggagcg aatatcgttc gtcagactgt cgggcagcgc aacatagtcc agctcggccg
    21541 ccatctgctt gccgttcttg aacgcccagt cgaagaactt cagcacttcg gcggactgag
    21601 ccttgtcggt cggctgcttg tacatcagga tgaaggacgc cgtcgacacc ggccaggact
    21661 tgtcgccctt ggcatccacc atcgacacgc ccatgcccgg cacgctgaac cagtccacac
    21721 ccgctgctgc cgacgcgaac gcctcatcgt cggggctcac gtacttgcca gccttgttct
    21781 gcagctgcat gaagttcatc ttgttctttt taacgtaggc gtactccaca taaccgacag
    21841 aacccttgat gcggttgacg ttggcggcca cgccctcgtt gcccttgccg ccgaccgacg
    21901 tcggggcggg ccatttgacc gcggcaccct tgcccacttt gtcggcgaaa tccttgctca
    21961 ccgtgctcag gtagtcggtc cagttgaacg tggtgcccga tccgtcagca cgatgcacca
    22021 cggtgatgac ttggtctggc aggttcttgc cggggttgag ggcggcgact ttggggtcgt
    22081 tccatttcgc gatattgccc aggaacatgt cggcaagcac ggggccggtg atgcgcagct
    22141 cgcccggctt gaaaccatcg aggttgatga ccggaacggt tccgccaatg atggccggaa
    22201 actgcaccat gccgtcttta tccagttctt cgcctttgac cggggcatcg gtggcaccaa
    22261 atgccacagt cttggcacgg atctggcgaa tgccgccgga cgaaccaatg gactgatagt
    22321 tcaggcccgt cccagtggca gccttgtagc cctcggccca cttggcgtag atcgggtacg
    22381 gaaaggtcgc gccggcgccc gtgatatcgg ccgctaaggc cgcggtgctg acgaaggcgc
    22441 ccagcgccgt gacggcca
//
LOCUS       DAOMVO010000008        22267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_381_length_22267_cov_16.310418, whole genome shotgun sequence.
ACCESSION   DAOMVO010000008 DAOMVO010000000
VERSION     DAOMVO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 22267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22267
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_22267_cov_16.310418"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..1190)
                     /locus_tag="PKV30_00970"
     CDS             complement(318..1190)
                     /locus_tag="PKV30_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRL28822.1"
                     /translation="MRWEGERQSDNVEDRRASGGGGGGFGIGGRSIGIGTIVIALLAW
                     GVFGINPLTTIGMLGGGGSPQVQQTPAQKPPASDQGAAFVSTVLASTEDAWNQVFKEG
                     GAQYRAPKLVLFRGLTPTACGTGQSAMGPFYCPNDQKVYLDMNFFDTLSRQLGAPGEF
                     ARAYVVAHEVGHHVQTLLGTTEKVDSMRGRISQSQQNQLSVRMELQADCYAGIWANKS
                     QQAKNWLEQGDIESAMNAAARIGDDALQRQQTGAVRPDSFTHGSSAQRQRWFMQGLKT
                     GSVQACDTFNTQNL"
     gene            1232..1918
                     /gene="pcp"
                     /locus_tag="PKV30_00975"
     CDS             1232..1918
                     /gene="pcp"
                     /locus_tag="PKV30_00975"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HRL28823.1"
                     /translation="MVAARPRARPSDKRAKSPSVLLTGFDPFGGDVLNPSWLIAQALH
                     RRQLAGHRIIAAQLPTVFGDSLTRLNALLAEHRPALVICLGLAATRAAISLERIAINV
                     DDARIADNAGAQPIDSPVIAGAPAAYFSTLPIKAMQQAISWAGVPAEVSQTAGTFVCN
                     HVFYGLMHRLATARGLKRTRGGFIHVPMLPEQGAPSLPLDQMVEGLRVGIRTALTVQQ
                     DVRAVGGAID"
     gene            2026..2949
                     /locus_tag="PKV30_00980"
     CDS             2026..2949
                     /locus_tag="PKV30_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HRL28824.1"
                     /translation="MDILLNPEQTAARLPWAPLVDEIEALLTDASVSVPPRTVLPMAA
                     GAFLFAMPGCDARTAMTKLISFTPANMGTGRPTIQGDVTVFDVASGERPLILDGPTVT
                     ARRTAAVSALAARWLAPDLNGPMLIVGAGVQGQAHIEVFAAALGVTQFRIASRRRASA
                     EALAEHARGLGLQAEVVDDADAALADCPLAATCTPASEVVLRAAPRADAFIAAVGAFT
                     PQMVEIDAELCRRIAAQGRIVVDSRDAGHEAGDLLQAGLDVDAMPALADIVRAQQKAR
                     AEGGPVLFKSCGWAGWDLAAARLAVQARAGR"
     gene            complement(2957..3289)
                     /locus_tag="PKV30_00985"
     CDS             complement(2957..3289)
                     /locus_tag="PKV30_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28825.1"
                     /translation="MATQIHSFDFQDTLAQAEPPSTVAPRRLRPPVVPGPEWLSPRQV
                     VTVVGLVALVVFLLACIAGVAKAQVRKGSEFQFAAQWPVVTAEAKVYSSRGGTNVADL
                     GPVAALSN"
     gene            complement(3376..3948)
                     /gene="dcd"
                     /locus_tag="PKV30_00990"
     CDS             complement(3376..3948)
                     /gene="dcd"
                     /locus_tag="PKV30_00990"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRL28826.1"
                     /translation="MSIKSDRWIRRMAEQHGMIEPFEPGQVREANGEKIVSYGTSSYG
                     YDIRCAPEFKVFTNIHSTVVDPKNFDEKSFVDINAPVCIIPPNSFALARTMEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFF
                     ESDVDDVCETSYKDRGGKYQGQVGVTLPKA"
     gene            4093..4833
                     /locus_tag="PKV30_00995"
     CDS             4093..4833
                     /locus_tag="PKV30_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL28827.1"
                     /translation="MLLLIFAAGLWAGLQNALAGGGSFVTLPALIMSGMSPLAANITS
                     TVALFPGQVTSGLAGRSLVTGAGRLPFKALFGISVVGGALGGLLLLNTPSSIFSRLVP
                     WLVLFATAMFAWGSFRRKPANLAAAQIGPVPTGIAQFFIAIYGGYFGGGIGFLMMAAL
                     SMAGVTARHAAATKNVLASVMNAAAVALFVTSPLVHWPQAIALGAGAILGGLAGVWAL
                     RRVNERWLRIAIVCIGLALTVGLFVKPV"
     gene            complement(4820..6142)
                     /gene="guaD"
                     /locus_tag="PKV30_01000"
     CDS             complement(4820..6142)
                     /gene="guaD"
                     /locus_tag="PKV30_01000"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HRL28828.1"
                     /translation="MKSWRASLLRFDEHGHAVFERDGLLVTGPGADGCAVVLAAGDHA
                     ALSPRYADVPCTHLPGRIIAPGFVDTHIHYPQLDVIGSPAEGLLPWLEHYTFPHEAQF
                     ADPAHATEVATFFCDELMRHGVTTALTFCTSHPASVDALFSEAQRRDLRLIAGKVLQD
                     RHSPDGVRDETEQSLIDTEALIKKWHGADRLGYAITPRFAPSCTEQQLRGAGELAARY
                     PRVWIQSHVAENVHEIEWARALFPDSRSYLAIYADHGLMRRRAMYAHCIHFDDDDRAL
                     MRETQTAAAVCPTSNLFLGSGFFDFAGAERVPFLHALASDVGGGASFSPFITMRAAYF
                     AARATSTGLQPKPGVSLSPEHLWWLHTAGAARALDLEGVIGNLQPGCEADFVVLNPEA
                     TPLLARRTRAAGSLAELLFALIVLGDDRVVERTVIAAPQQGAPSLRPA"
     gene            complement(6323..7327)
                     /locus_tag="PKV30_01005"
     CDS             complement(6323..7327)
                     /locus_tag="PKV30_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL28829.1"
                     /translation="MNPIFKENTAPALALIAGAALFSVAFNASAQVNYPAKPIKMIVP
                     FQAGGATDVMARVVGQKLSAQLGQPVVIDNKAGAAGIIGTDAVAKAPPDGYTIMLGLS
                     NSLMTNQFLYTKLPYDAQKDLAMVYRIAMAPLVLVVNPSVPAKTGPELLKYVAANKGK
                     VSYGSYGIGAYPHLAGAHMSLTQNADMVHVAYKGEAPMVQDLIGGQIQMAFASALQVK
                     GHLDAGKLRAIGTSGEKRMATLPQVPTPAEQGLNDEAYRVTGWLAVAAPGATPQPIID
                     KLASEIQKAVQSPEVRERIVGMGFEVQDNGLPPAFNAAYRQELPIWYRLIKQSGAKLD
                     "
     gene            complement(7375..9306)
                     /locus_tag="PKV30_01010"
     CDS             complement(7375..9306)
                     /locus_tag="PKV30_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRL28830.1"
                     /translation="MSPSAPLSPPSEFAPPFSATTRVTSLADVRQIEATPLSEALTVR
                     STYEIFRNSAHTFGDKTALGFLRTADPAEEPVRWSYSQLLAGIHQTANALHALGVGPT
                     DAVAVMLPGCLEYHLALWGGEAAGIVQPLNPLLTDDKLASLLNASRAKVLIAWGDDAE
                     SGIWSKAEHLRATVPTMTHVLRVAPLDERQPPALPEGVLDFAATIAAQPDTHLASGRD
                     IGPDDIAAYFHTGGTTGAPKLARHSHRAQVFTAWACVQMQAMGPQDVVINGYPLFHVA
                     GTLPGSLAAFSCGVETIIPTTGLYRNRAVLQNFWRLVEKYRVTTLSAVPTVLAALAQV
                     PLAGADISSVRYCRTGAAILPPELAARFEREFGLHVHESLGMTEMAGISTITPLGVNG
                     LPGCVGFRLPHTQMRIVELDADGGASGRDVAPGEPGMVLFKSPNVFSGFLDAADTAKA
                     FTPDGWLITGDIGYLDRDERLNLSGRAKDLIIRSGHNIDPKTIEDAIGAHPAVQLAAA
                     VGAPDAYAGELPVVFATLRPGAQVSEAELLAFTAERVDEAPAKPKHVYLIEQMPVTNV
                     GKIYKPELRAQAAGHTARELIAALCQALPPDQRPRVASTEASGVAVHLPPGAPAALAE
                     RLQAALAPLPVKTRVIVGA"
     gene            9572..10120
                     /locus_tag="PKV30_01015"
     CDS             9572..10120
                     /locus_tag="PKV30_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL28831.1"
                     /translation="MRQKMVAGATQVLARRGLHATAFSEVLALTGASRGSIYHHFPGG
                     KAELIEAVLDRHASDIDDGLRALHGLPVDQVVSGALDVWRARLVRDDCESGCPVAAVT
                     IAADTSRAVEDCRGAFRQWQDTLASALRAAGCSPRRASDSATLLVAAVQGALILARAE
                     GQTAPFDTVAGLLRAQTGEWVA"
     gene            complement(10135..11205)
                     /locus_tag="PKV30_01020"
     CDS             complement(10135..11205)
                     /locus_tag="PKV30_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL28832.1"
                     /translation="MYKNLAATLVAACFLSPAFSQSPAPVKAAWVYVTPLTDAGWTQQ
                     HDQGRRAVEKALGSQVNTTYVENVPEGADAERVIRDLAAQGNQIIFTPSFGYMEPTLK
                     VARDFPGVKFESVTGLKQAPNVATANARYYEGRYLSGIAAGRMTRSNVAGYVAGFPIP
                     EVLQGINAFTLGLRSVNPKATVKVIWLNEWFNPPREREAAMTLMNQGADVLAFHTGST
                     AVMAAAEERGKLAVAYHADMRGAAPTAQIAAVTHQWGDYYTRRVKAAHDGSWRSGNLW
                     GGVREGMIRVEAFGPKVPQRVQEEVLARQKDMAAARLHPFAGPLADNTGRALLPRGQT
                     LSDAQILQMNWLVDGVQTRLPH"
     gene            complement(11378..12280)
                     /locus_tag="PKV30_01025"
     CDS             complement(11378..12280)
                     /locus_tag="PKV30_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28833.1"
                     /translation="MKPDPFDAAHDPRDPNPWLANYLDQSTPLDDAVKRAWLADSASG
                     SRQYLLPIIRPLARACIVLIQVLKIAMPKKWAASLMLHRILAWGMNRCLSPQANWLIL
                     RHFHLGSQVLAFIAANSGVQVTLSPLTPMVIDDLKDEVFLKHDLNLFNFIINLNRALR
                     AEGRTLKPVPMAEVDFSMLHQPALALQDMPHGKLNGIDLQSAIEIFTPVYQLFLTDHD
                     FWRASNSLQLDETIGIYAATLLNSPQHLVLLNNKHPLVPMSTLRAGFRLVLHGLSTEM
                     LHCLLMEMKDAQEAARVTARTVAF"
     gene            complement(12284..14182)
                     /locus_tag="PKV30_01030"
     CDS             complement(12284..14182)
                     /locus_tag="PKV30_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HRL28834.1"
                     /translation="MPVRFDNVHLNGLGLYLPGPPIGNDAMDDYVAPLNRASARIKQR
                     ILTENGIQTRHYAIDPDGRTRESHASLAANAIRACLQSSRATLGDVSLLAVGSSGGDA
                     LMPGFANMIQGELAAPPMQTLSSQGVCVAGVSALEFAAQAVALGSHAHALAVGADMPS
                     RMFKHTRFAPAGYQTDFDAHFLRWMLSDGAGACLLGRTPQPIGDSGRALKLKWLHQKS
                     FSGDYPVCMQLGLTHDRSRSFLDFASSSEAEAAGALALRQDIRLLPHVFDVGIHEYVK
                     LAHEGWIEPARTDHFLCHYSSAKFIPVIRDMLDKADLAIDDDKWWSNLATRGNTGAAS
                     IFVMLAEWFTSVQPKAGETVLCFVPESGRMTAAFMAFEVVDCKQNMPLPPTQQARAAI
                     NTEANDTIAPAAVAEPHAPADAPAALRPVLTALAGVWQDYRSGVWRTPIVQQLVHGRF
                     DVAAYRRWTQHWVPQVREGSRWMREAVAQLDARFLPLAALIGQHAGEEQDDFKILYDD
                     YRAAGGTLALDELARNPGGEALNAYLHRLAAEANPVGLLGAIYIIEGTGQRIVPTLLP
                     LLREQVKLPASAFRFLAYHGANDEHHLARWLQAVDIVLAIHPAAAAQIVQTARHTAQL
                     YSLQFELI"
     gene            complement(14166..15083)
                     /locus_tag="PKV30_01035"
     CDS             complement(14166..15083)
                     /locus_tag="PKV30_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRL28835.1"
                     /translation="MARWLKHPSDWRSVLCIALWPLCVAALYTGRLPGWALGLVLGLL
                     IYLSLALPVIEHNHMHLRLWRTHRLNRLTDTLLSALQGRPVFLFHPAHNANHHRYHHG
                     PRDAARTYRFAGGDTNHLWGYLLHPLQAVGVLWPLFGAWLARLTARAPAVRRYYLRQI
                     AWAVAFTALMIAIDPAAWLKFVLLPQLLGLHWLLGANYLQHAHADGRSRLQFARNFTG
                     GVNRWFFNIGYHTAHHLQPRLHWSALPAAHQRIASQIDPRLTERSLLRYALVTLVGGL
                     IWRGARSHSLMQAAPPSQRDSGEPIARSL"
     gene            complement(15086..15841)
                     /locus_tag="PKV30_01040"
     CDS             complement(15086..15841)
                     /locus_tag="PKV30_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRL28836.1"
                     /translation="MTHVSLLDLTWYQVVAFGLAYFSAIYLLLGALTLWLTRRVLPML
                     GHGSVLDRRAVPTGQLAREWRLSALSIVIFGTGLLVPWWLLKLGWARIDDQAGALQIA
                     LEVLALTLWNEVHFYANHRLLHTRWFKRFHLPHHRSVLTTPWATYSFHPLEAVLLGNV
                     ILLPMVLHDFSLPALLALPIISLVFNNIGHSNYDFLPGAAHDRWWLNGARRHHLHHAC
                     YSGNFGFMLPFMDRWFGTNLPPDAALARRAARP"
     gene            complement(16042..17091)
                     /locus_tag="PKV30_01045"
     CDS             complement(16042..17091)
                     /locus_tag="PKV30_01045"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRL28837.1"
                     /translation="MKLPAIAPDRLPALLAAMPKAELHMHIEGSLEPEMIFALAERNG
                     VALPYASIEELRHAYAFTDLQSFLDIYYAGASVLLKEEDFYDLAQAYLARAATENVVH
                     AEIFFDPQTHSARGVPMETVIRGLSRACADARAQGTSAELILCFLRHLSEREASETLE
                     AALPFRDHFIGVGLDSSEVGHPPEKFARVFARCRELGFHIVAHAGEEGPPAYIWQALD
                     VLKAERIDHGVQAVHDAALMQRLAGERVPLTVCPLSNQKLRVFPDLAQHNLKRLLDAG
                     LCATVNSDDPAYFGGYINANFQQVFAATGMTAEQAWQLAANSFEASFAPAADKARWLA
                     RLQTTFERFAAPAEI"
     gene            complement(17164..18240)
                     /locus_tag="PKV30_01050"
     CDS             complement(17164..18240)
                     /locus_tag="PKV30_01050"
                     /inference="COORDINATES: protein motif:HMM:NF019123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1521 domain-containing protein"
                     /protein_id="HRL28838.1"
                     /translation="MFATSQTGFVAIAAVGFAPIGAADRPRCVLPHTNRFQLGSVDKK
                     GCFCPKPDAQWTVKNPGNGTANIDLGKYELQLDEKNSQIKIVNKNNGEVTNIWGDPHI
                     DWNKDGKTDANFWGKTTFQLEDGTKITIDTEPFKNNPNEYVSSEVTVTRGDHAMKITG
                     LSQNELGDLKVEYADRGGQALDWATTDGFVVRENACGEGWINNDTGKLATQQDFNITK
                     PGTYKPYEFTQQFGQALGLFLMTGWMSGGLSLFGGGSCWNTGESRTSEPPRNPYLKPL
                     PATRGRVEQPDANTLAIHHRNGSVDTRSGGTLAWRANNPGLLPYNPITRGMGAIGAVD
                     GVAVFANESSGRAALAKLMLGLLR"
     gene            complement(18401..19399)
                     /locus_tag="PKV30_01055"
     CDS             complement(18401..19399)
                     /locus_tag="PKV30_01055"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRL28839.1"
                     /translation="MIERSLWHPVALSHAVGSQPQGASLLGEDLVLWRDAAGAAHAWA
                     DQCPHRGARLSLGRVAGGQLECPYHGWRFEGSGQCTHIPALPDFTPGARHRARSFDAR
                     EAYGMLWVRLAPPPADAPPIHDFPLFAAESEPRLRKTTSGPYRVEASAPRIIENFLDM
                     AHFGYVHEGWLGSRDRPEIPPYEVAPTPHGVLATGCLAWQPRSSIHAESGAQVEYTYE
                     VTAPYMAVLTKKPEPGSTAIEGLWESIAMFVCPVTEESSVAWTRMAMNDFDSPDARLI
                     DFQNTIFGQDQPVLESQRPKRLPLRPDAEVHSAADRMSAAYRRYLKESGIGFGVIA"
     gene            complement(19458..20630)
                     /locus_tag="PKV30_01060"
     CDS             complement(19458..20630)
                     /locus_tag="PKV30_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL28840.1"
                     /translation="MTHFASRRSLLRLTAAAAALGAAALTGCGKKDEAPAAPAASAET
                     AAPPAPAPAAEPLKAAWIYVGPVGSAGWSYAHDLGRKAAEAEFGDRLKTTFIESVPEG
                     ADTERVLRDLVAQGNKVIFGTSFGFMEPMLKVAKDFPDVKFEHATGYKTADNMNVYDS
                     RFYQDTYLAGIVAAHMTKTGTLGFVGSFPIPEVLRNINAFTLGAQSVKPGIKVRVVWV
                     NTWYDPPKETEAAQALMNGGADVLLQNTDSTAVLQAAEKAGKRAFGWDSDMSAFGGSA
                     HLGSAIADWSHYYKKTLKDVIDGTWKGGQVTRWGVPEGQNDLIKLAEDIPAEAKAKVD
                     EIKAKMKDRSFDVFTGPVVDGDGKERLAAGTKADDDWMGKIDFYVKGVEGKVPASK"
     gene            complement(20885..22054)
                     /locus_tag="PKV30_01065"
     CDS             complement(20885..22054)
                     /locus_tag="PKV30_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL28841.1"
                     /translation="MTDQHKRSILKLAALAAAASALVACGKKDEPAPTAAAPAPAPAA
                     ASAPAPAKADPLKIAFAYVGPVGDGGWTFAHDNARKALEKEYGDKIVTSFVESVPESA
                     DAERVIRDMAGQGNKLIFGTTFGYMEPMLKVAADNPEVKFEHATGYKTAPNMRTYDSR
                     TYEGAYMAGVIAGRMTKSNTLGVVASIPIPEVIRNINSFTLGAQSSNPNIKTKVVWVN
                     EWFNPPKETEAATALINGGADILFQNTDSSAVLQTAEKMGKRGFGWDSDMTAYGPKAH
                     LGSAIINWAPYYIKTVGEVLDGKWATGQTWWGVKEGAIDLVSLAADVPEEAKKALDDV
                     KAGLKAGSYNIWKGPISDNTGKEVLAKDAVADDKFLGGINFFVKGVEGKVPGDKK"
BASE COUNT         3431 a         7211 c         7748 g         3877 t
ORIGIN      
        1 aaatgtgtca cccctgtgca gcttacgcgg cttccccaag gggacgccgt ctgcgaccgg
       61 gccaagcccg ctccgcgccg gctcgaagtg gcctgctgcg cggccacagc gggcaagctg
      121 cagtcacttc gttttttata gctgctcgcg cttgccagtc aagcgctggc ggccaaaaac
      181 acccataaaa gaggctgcgc agcaagccgc cttcaaatgg ccgcggagca ggccacacgg
      241 gaacgctgcg gcacgggctt accccggccg ccagcgttgt cccccttggg gggaagacgc
      301 cgaaagcggc acaggggtta caggttctgt gtattaaagg tgtcgcaagc ctgcacgctg
      361 cccgtcttca gcccctgcat gaaccagcgc tggcgctgag cgctgctgcc gtgggtgaaa
      421 gagtcgggcc gcacggcacc ggtttgctgg cgctgcaggg catcatcgcc aatgcgcgcg
      481 gctgcgttca tggccgattc gatgtcgcct tgctccagcc agttcttggc ctgttgcgac
      541 ttgttggccc agatgccggc gtagcagtcg gcctgcagtt ccatgcgcac cgacagttgg
      601 ttctgttgcg actggctgat gcggccacgc atgctgtcga ccttctcggt tgtgcccagc
      661 agtgtttgca cgtggtggcc gacctcgtgc gccaccacgt aggcgcgcgc gaattcgccc
      721 ggcgcgccga gctggcggct cagcgtgtcg aagaagttca tgtccagata caccttctgg
      781 tcgttcgggc agtaaaacgg ccccatcgcg ctttgcccgg tgccgcacgc ggtgggcgtc
      841 aggccgcgaa acagcaccag cttgggcgcg cggtactgcg cgccgccttc cttgaagacc
      901 tggttccacg cgtcctcggt cgaggccagc acggtcgaca caaaggccgc gccctgatcg
      961 ctggcgggcg gcttttgcgc cggcgtctgc tgcacctgcg gcgagccgcc accgcccagc
     1021 atgccaatgg tggtgagcgg gttgatgccg aacacgcccc aggccagcag cgcgatcacg
     1081 atggtgccga tgccgatgct gcgcccgccg atgccaaagc cgccgccccc gccgccactg
     1141 gcgcggcggt cttccacgtt gtcggactgt ctttcgcctt cccatctcat gctcgactcc
     1201 tcgtgcgcaa ggtttgcgct aaattgcggc aatggtagct gcccgccccc gtgcccgccc
     1261 gtcagacaag cgcgctaaaa gtccgtcggt gctgctgacc ggcttcgatc cctttggcgg
     1321 cgacgtcctc aaccccagct ggctgatcgc ccaggcgctg caccggcggc agctggcggg
     1381 ccaccgcatc atcgccgcgc aactgccgac ggtgtttggc gattcgctca cgcgcctgaa
     1441 cgccttgctg gccgaacacc ggccggcgct cgtcatctgc ctgggcctgg ccgccacgcg
     1501 cgcggcgatc tcgctcgaac gcatcgccat caacgtcgat gatgcgcgca ttgccgacaa
     1561 cgccggcgcc cagcccatcg atagccccgt gatcgcgggg gcgccggccg cgtacttcag
     1621 cacgttgccg atcaaggcga tgcagcaggc gatcagttgg gcgggcgtgc cggccgaggt
     1681 gtcgcaaacc gccggcactt ttgtctgcaa ccacgtgttc tacgggctga tgcaccggct
     1741 ggcgacggcg cgcggcctca agcgcacgcg cggcggcttc atccacgtgc ccatgctgcc
     1801 cgagcagggc gcgcccagcc tgccgctgga tcagatggtc gaaggtctgc gcgtcggcat
     1861 acgcacggcg ctgacggtgc agcaggatgt gcgcgcggtt ggtggcgcca ttgattgata
     1921 aaacagggcg ccagcgcttg tctggcaagc gcaagcagct agcaaaattg aagtaatgcc
     1981 ataggccgcc catgctgcgg tagcggtgcc gcagaatctc agcccatgga cattctgctc
     2041 aatcccgaac aaaccgccgc acgcctgccc tgggcgcctc tggtcgatga aatcgaagcg
     2101 ctgctgaccg acgccagcgt gtcggtgccg ccgcgcaccg tgctgccgat ggccgccggc
     2161 gcctttttgt tcgccatgcc gggctgcgac gcgcgcacgg cgatgaccaa gctgatcagc
     2221 ttcacgcccg ccaacatggg caccggccgg ccgacgattc agggcgacgt cacggtgttc
     2281 gacgttgcca gcggcgagcg gccgctgatc ctcgacggcc ccaccgtcac cgcgcgccgc
     2341 accgctgctg tctcggcgct ggccgcgcgc tggctggcac ccgacctgaa tggcccgatg
     2401 ctgatcgtcg gtgccggcgt gcaaggccag gcgcacatcg aggtgtttgc cgccgcgctg
     2461 ggcgtgacgc agtttcgcat tgcctcgcgc cgccgcgcca gcgccgaagc cctggccgaa
     2521 cacgcgcgcg gcctgggcct gcaggccgag gtggtggacg atgccgacgc cgcgctggcc
     2581 gactgcccgc tggccgccac ctgcacgccg gccagcgagg tggtgctgcg cgccgcgccg
     2641 cgcgccgacg ccttcatcgc cgccgtcggc gccttcacgc cgcagatggt cgaaatcgat
     2701 gccgaactgt gccgccgcat cgccgcacag ggccgtatcg tggtcgattc gcgcgatgcc
     2761 gggcatgagg cgggcgatct gctgcaggcc gggctggacg tggatgcgat gccggcgctc
     2821 gccgacatcg tgcgcgcgca gcaaaaagcc cgcgctgaag gcgggccggt gctgttcaag
     2881 agctgcggtt gggccggctg ggacttggct gccgcgcggc tggccgttca agcgcgcgct
     2941 gggcgttgat gccgcctcag ttcgacagcg ccgccaccgg ccccagatcg gccacgttgg
     3001 tgccgccgcg gctgctgtag accttggcct cggccgtgac gaccggccac tgggcggcaa
     3061 actggaattc gctgcccttg cgcacctgcg ctttcgccac gccagcgatg caggccagca
     3121 agaaaacgac cagcgccacc aggcccacca ccgtgaccac ctgccgtggc gacagccatt
     3181 ctgggcccgg cacgactggc gggcgcaagc ggcgcggtgc aactgtcgaa ggcggctcgg
     3241 cctgcgccag cgtgtcctgg aaatcgaatg aatgaatctg cgtagccatg cgccgattct
     3301 gcgaaggcca cggcgcggtg ccgctcagcg ggcggcgggc ccgcaagtag gagaaaaccg
     3361 aagcccggcg cgggctcagg ccttgggcag cgtcacgccc acctggccct ggtacttgcc
     3421 gccgcggtcc ttgtacgagg tctcgcacac gtcgtcgacg tcgctctcga agaacagcac
     3481 ctgcgcgcag ccttcgccgg catagatctt ggccggcagc ggcgtggtgt tgctgaactc
     3541 gagcgtcacg tagccttccc actcgggctc gaacggcgtg acgttgacga tgatgccgca
     3601 gcgcgcgtaa gtgcttttgc ccaggcaaat ggtcagcacg ttgcgcggga tgcggaagta
     3661 ttccatcgtg cgcgccagcg caaagctgtt gggcgggatg atgcagaccg gcgcgttgat
     3721 gtcgacaaag cttttctcgt cgaagttctt cgggtccacc accgtcgagt ggatgttggt
     3781 gaagaccttg aattcgggcg cgcagcggat gtcgtagccg tagcttgagg tgccgtagct
     3841 gacgatcttt tcgccgttcg cctcgcgcac ctggccgggc tcgaagggtt cgatcatgcc
     3901 gtgctgctcg gccatgcggc ggatccagcg gtcgctcttg atgctcatcg aatactacct
     3961 tttttatagc ttgttgcgct tgtgcggcaa gcactggagc acgattttgc ctgatttcac
     4021 cgtgcgccgg ccgcccgagg cgcgttggtg tcatgatcgc ctgccctgcc gccacttgcc
     4081 acgccgatgc tgatgcttct tctgattttc gccgcaggcc tgtgggccgg actgcagaac
     4141 gcgctggcgg gcggcggctc cttcgtcacg ctgccggcgc tgatcatgtc gggcatgtcg
     4201 ccgctggcgg ccaacatcac ctccacggtg gcgctgtttc cggggcaggt cacgtcgggt
     4261 ctggcaggtc gcagcctggt gacgggggcg gggcggctgc cgttcaaggc gctgttcggc
     4321 atcagcgtgg tggggggcgc gctcggcggc ttgctgctgc tgaacacgcc gtcgtccatc
     4381 ttttcccggc tggtgccgtg gctggtgttg ttcgctaccg cgatgttcgc gtggggcagc
     4441 tttcgtcgca agccggccaa cctggcggcc gcacagatcg gcccggtgcc gaccgggatt
     4501 gcgcagttct tcatcgccat ctacggcggc tatttcggtg gcgggatcgg cttcctgatg
     4561 atggcggcgc tgtcgatggc gggcgtgacg gcacgccacg cggcggccac caagaacgtg
     4621 ctggcctcgg tcatgaacgc ggcagcggtg gcgctgttcg tcacctcgcc gctggtgcac
     4681 tggccgcagg cgatcgccct gggcgccggc gccatcctgg gcggcctggc cggcgtgtgg
     4741 gcgttgcgcc gcgtgaacga gcgctggctg cgcatcgcca tcgtgtgcat cggcctggcg
     4801 ctgacggtgg ggctgttcgt caagccggtc tgagcgatgg cgctccttgc tgcggcgccg
     4861 cgatgaccgt gcgctcgacc acccggtcgt cgcccagcac gatgagcgcg aacagcaact
     4921 cggccaggct gcccgccgcg cgcgtgcggc gcgccagcag cggtgtggct tcggggttca
     4981 gcaccacgaa gtcggcctcg cagccgggtt gcagattgcc gatcacgcct tccagatcca
     5041 gcgcgcgcgc cgcgcccgcc gtgtgcagcc accacaggtg ttcgggtgac aggctgacgc
     5101 cgggcttggg ctgcaggccg gtgctggtgg cgcgcgcggc aaagtaggcc gcccgcatgg
     5161 tgatgaaggg cgaaaagctg gcgccgccgc cgacatcgct ggccagtgcg tgcaagaagg
     5221 gcacgcgctc ggcgccggca aagtcgaaga agccgctgcc cagaaacaga ttgctggtcg
     5281 ggcacacggc ggcggcggtt tgcgtctcac gcatcagcgc gcggtcgtcg tcgtcaaagt
     5341 ggatgcaatg cgcgtacatg gcgcgccggc gcatcaggcc gtggtcggcg tagatggcga
     5401 gatagctgcg cgaatcgggg aacagcgcgc gcgcccactc gatctcgtgc acgttctcgg
     5461 ccacgtgcga ttggatccat accctggggt atcgcgccgc cagctcgccg gcgccgcgca
     5521 attgctgctc ggtgcagctc ggcgcaaagc gtggcgtgat ggcgtagccc aggcgatcgg
     5581 cgccatgcca ctttttaatg agcgcttcgg tgtcgatcag gctttgctcg gtctcgtcgc
     5641 gcacgccatc gggtgaatgc cgatcttgca gcaccttgcc ggcgatcagg cgcagatcac
     5701 gtctttgtgc ttcagaaaaa agagcatcga cagatgccgg gtgtgaggtg cagaaggtca
     5761 gcgccgtggt gacgccgtgg cgcatcagct catcgcagaa gaacgtggcg acctcggtgg
     5821 cgtgcgccgg gtcggcaaac tgcgcttcgt gcggaaacgt gtagtgctcc agccagggca
     5881 gcaggccctc ggccggcgag ccgatcacat ccagctgtgg atagtggatg tgggtgtcca
     5941 caaagcccgg cgcgatgatg cggccgggca ggtgggtgca gggcacgtcg gcgtagcggg
     6001 gcgacaaggc cgcgtggtcg cccgcggcca gcaccacggc gcagccatcg gcgccggggc
     6061 cggtcaccag caggccatcg cgctcgaaca cggcgtggcc gtgttcgtcg aaccgcagca
     6121 gggacgcgcg ccaggatttc atgcgcgcat tgtgccgcgc cgggcgggcg tgaacaggcg
     6181 gtgccttttc gcgtgcaccg ttcgggctga gctggtcgaa gccagcgctt cgacaggctc
     6241 agggcgaagg gtggggcgga cccgccggtg gcattcgcgg cgcggtggca ctcatgcaag
     6301 tgctgcgcac gcgcactcca catcaatcca gcttggcgcc cgattgcttg atcagccggt
     6361 accagatcgg cagttcttgc cggtacgccg cgttgaaggc aggcggcagc ccgttgtcct
     6421 gcacttcaaa acccatgccg acgatgcgtt cgcgcacttc gggcgactgc acggctttct
     6481 ggatttcgga cgccagcttg tcgatgatgg gctgcggcgt ggcgcccggc gcggccacgg
     6541 ccagccagcc ggtcacgcgg taagcctcgt cgttcaaacc ctgctcggcc ggcgtgggca
     6601 cttggggcaa cgtggccatg cgcttttcac cgctggtgcc gatggcgcgc agcttgcccg
     6661 cgtccagatg ccccttgacc tgcagcgcgc tggcaaaggc catctggatc tggccgccga
     6721 tcaagtcctg caccatgggc gcctcaccct tgtaggccac gtgcaccatg tcggcgttct
     6781 gcgtcaggct catgtgcgcg ccggccaagt gcgggtaggc gccgatgccg tacgagccgt
     6841 acgacacctt gcccttgttg gcggccacgt acttgagcag ttccggcccg gtcttggccg
     6901 gcacgctggg gttgaccacc agcaccagcg gcgccatggc gatgcggtag accatggcca
     6961 agtccttctg cgcgtcgtac ggcagcttgg tgtagaggaa ctggttggtc atcagcgagt
     7021 tggacaggcc cagcatgatg gtgtagccat cgggcggcgc cttggccacc gcgtcggtgc
     7081 cgatgatgcc ggccgcgccc gccttgttgt cgatcaccac cggttgaccc agctgcgccg
     7141 acagcttctg cccgaccacg cgcgccatca cgtcggtggc gccaccggcc tggaagggca
     7201 cgatcatctt gatcggcttg gccggatagt tgacctgcgc gctggcgttg aatgctactg
     7261 aaaaaagagc tgctcccgca atcagggcaa gcgccggagc ggtattttcc ttgaagatcg
     7321 ggttcatgat ggcttgatgg gggtgggtgg aacgaaacgg gagggcccgg ccgctcaggc
     7381 gccgacgatg acgcgcgtct tcacgggcag cggcgccagc gcggcctgca accgttcggc
     7441 cagcgccgct ggcgcacccg gcggtaggtg caccgccacg ccgctggcct cggtgcttgc
     7501 cacgcgcggc cgctgatcgg gcggcagcgc ctggcagagc gccgcgatca gctcgcgcgc
     7561 cgtgtggccc gcggcctgcg cgcgcagctc gggtttgtaa atcttgccga cgttggtgac
     7621 gggcatctgc tcgatcaggt acacgtgctt gggcttggcg ggagcctcgt ccacccgctc
     7681 ggcggtgaag gcgagcagtt cggcctcgct cacctgcgcg ccggggcgca gcgtggcaaa
     7741 gaccaccggc agctcgcccg cgtaggcatc cggcgcgccg acggcggcgg ccagttgcac
     7801 ggcggggtgc gcgccaatcg cgtcctcgat cgtcttgggg tcgatgttgt ggccgctgcg
     7861 gatgatcaag tccttggcgc ggccgctcag gttgaggcgc tcgtcgcgat ccaggtagcc
     7921 gatgtcgcct gtgatcagcc agccgtcggg tgtgaaggcc ttggcggtgt cggccgcatc
     7981 cagaaaaccc gagaacacgt tgggcgactt gaacaacacc atgcccggct cacccggcgc
     8041 cacgtcgcgc ccgctggcgc cgccatcggc atccagttca acgatgcgca tctgcgtgtg
     8101 cggcaggcgg aaaccgacgc agcccggcag cccgttcacg cccagcggcg tgatcgtgga
     8161 aatgccagcc atctccgtca tgcccaggct ttcatgcacg tgcaggccga actcgcgctc
     8221 aaagcgcgcc gccagctcgg gcggcaggat ggcggcgccg gtgcggcagt agcgcacgct
     8281 ggagatgtcg gcacccgcca gcggcacctg cgccagcgct gccagcaccg tgggcacggc
     8341 cgacagcgtg gtcacgcggt atttctccac cagacgccag aagttctgca gcaccgcgcg
     8401 gttgcggtac aggccggtgg tcgggatgat ggtttccacc ccgcacgaga acgccgccag
     8461 cgagcctggc agcgtgccgg cgacgtggaa cagcgggtag ccgttgatga ccacgtcctg
     8521 cgggcccatg gcctgcatct gcacgcaggc ccaggcggtg aacacctgtg cgcggtggct
     8581 gtggcgcgcg agcttgggcg cgccggtggt gccgccggtg tggaagtagg cggcgatgtc
     8641 gtcggggcct atatcgcgtc cgctcgccag gtgcgtgtcc ggctgcgcgg cgatggtggc
     8701 ggcgaagtcg agcacgcctt cgggcagcgc gggcggctgg cgctcgtcca gcggcgccac
     8761 gcgcagcacg tgcgtcatcg tcggcaccgt cgcgcgcaaa tgctccgcct tgctccagat
     8821 gcccgactcg gcgtcgtcgc cccaggcgat cagcaccttg gcgcgcgagg cgttcagcag
     8881 cgacgccagc ttgtcgtcgg tcagcagcgg attgagcggc tgcacgatgc ccgccgcctc
     8941 gccgccccag agcgccaggt ggtattcaag gcagccgggc agcatcaccg ccaccgcgtc
     9001 ggtaggcccc acgcccagcg cgtgcagcgc gttggcggtc tggtgaatgc cggccagcag
     9061 ttggctgtac gaccagcgga ccggctcttc ggccggatcg gccgtgcgca gaaacccaag
     9121 cgcggtcttg tcgccaaaag tgtgcgccga attgcgaaaa atctcgtagg tgctgcgcac
     9181 cgtcagcgcc tcgctcaagg gcgtggcttc gatctggcgc acgtcggcca ggctggtcac
     9241 gcgcgtggtg gctgaaaacg gtggcgcgaa ctcgctgggc ggcgaaagcg gggcgctggg
     9301 gctcatgggc tgtctcctgc cggccatcgg agcgccgccg gcggcaaacc gtcgaaacag
     9361 ggcgtcacgg cggcgactgg catcaagtcg ccagccttca ttgcttttat aagaatagca
     9421 acaaacacag gtgcaacaag gccctatgtc gaatgacata gtaagacgtc ggtctaactg
     9481 ctattctgcc ggcccttgtt ccagctccct ggtcaacgtt tccaagtacg gtggttttgt
     9541 gcaagacgat tcggtcccca cacgcggcag catgcgtcag aaaatggtgg ccggcgccac
     9601 ccaggtgctg gcgaggcgcg ggctgcatgc cacggctttt tcggaagtgc tggcgctgac
     9661 gggcgcctcg cgcggctcca tctaccacca ctttccgggc ggcaaggccg agttgatcga
     9721 ggcggtgctg gaccgccacg ccagcgacat cgacgacggc ctgcgtgcct tgcacggcct
     9781 gccggtcgat caggtggtga gcggtgcgct cgacgtctgg cgcgcgcgct tggtgcgcga
     9841 cgattgcgaa tcgggctgcc ccgtggcggc ggtgaccatt gccgccgaca catcacgcgc
     9901 cgtcgaggat tgccgtggcg ctttcaggca atggcaggac accctggctt cggcgctgcg
     9961 cgccgcaggc tgctcgccgc ggcgcgccag cgacagcgcc accttgctgg tggcggcggt
    10021 gcaaggcgcc ctcatcctgg cgcgcgccga aggtcaaacc gcgcccttcg acaccgtggc
    10081 cgggttgctg cgtgcgcaga cgggcgagtg ggtggcctga cgcgggccgg cgcttcagtg
    10141 cggcaggcgc gtctgcacgc cgtcgaccag ccagttcatc tgcaggattt gcgcgtcgct
    10201 cagagtctgt ccgcgaggca gcagcgcgcg gccggtgttg tcggccagcg ggccggcaaa
    10261 cgggtgcagg cgagcggcgg ccatgtcttt ctggcgcgcc agcacttcct cctgcacgcg
    10321 ctggggcacc ttggggccaa aggcttcgac gcggatcatg ccttcgcgca cgccgcccca
    10381 caggttgccg ctgcgccatg agccgtcgtg cgccgccttc acccggcgcg tgtagtaatc
    10441 gccccactgg tgcgtgacgg cggcaatctg cgccgtgggc gcggcgccgc gcatgtcggc
    10501 gtggtaggcc acggcgagct tgccgcgttc ctcggcggcg gccatcacgg cggtggagcc
    10561 ggtgtgaaac gccagcacgt cggcaccctg gttcatcagc gtcatggcgg cctcgcgctc
    10621 gcgcggcggg ttgaaccatt cgttcagcca gatcaccttg accgtggctt tggggttcac
    10681 gctgcgcagg cccagcgtga aggcgttgat gccctgcagc acttcgggga tcggaaagcc
    10741 agccacgtag ccggccacgt tggatcgggt catgcgcccc gccgcgatgc cggacaggta
    10801 gcgcccctcg taatagcgcg cgttggcggt ggccacgttg ggcgcctgct tgaggccggt
    10861 gacggattcg aacttgacgc ccggaaaatc gcgcgccacc ttcagcgtcg gctccatgta
    10921 gccgaagctg ggggtgaaga tgatctgatt tccctgcgcc gccaggtcgc ggatcacacg
    10981 ctcggcgtcg gcgccttcgg gcacgttctc gacgtaagtc gtgttgacct gactgcccag
    11041 cgctttctcg accgcgcgcc ggccctggtc gtgctgctgt gtccagccgg cgtcggtcag
    11101 cggcgtgacg tagacccacg cggccttgac gggcgcgggc gattgcgaaa aggcgggaga
    11161 taaaaaacag gcggccacga gcgtggcggc gaggtttttg tacatggtgt tcctctacat
    11221 ggccccgaag acggaaaaag acccaagacc gcggggcacg gtctcgggtt gcttggtgat
    11281 tttaaggcgc agccgtcaca gccaggagtg atcgatggtt gatatcaaaa taatagctgc
    11341 ccgcgcttgg tagataagcg ctgaagactg tttttgatca gaaggccact gtccgggctg
    11401 taacgcgcgc agcctcctgc gcgtccttca tctccatcag caggcagtgc agcatctcgg
    11461 tggacagccc gtgcaacacc agccgaaagc cggcgcgcag cgtgctcatg ggcaccagcg
    11521 ggtgcttgtt gttgagcagc accaggtgct gcgggctgtt gagaagggtg gccgcataga
    11581 tgccgatggt ttcatccagt tgcagcgaat tggaggcgcg ccagaagtcg tgatcggtca
    11641 gaaacagctg gtacaccggc gtgaagattt caatggccga ttgcaggtcg atgccgttga
    11701 gcttgccgtg cggcatgtcc tgcagcgcca gcgctggttg atgcagcatg ctgaagtcca
    11761 cctcggccat cggcaccggc ttcagtgtgc ggccttcggc gcgcaaggcg cggttgaggt
    11821 tgatgatgaa gttgaacaag ttgaggtcgt gcttgaggaa cacctcgtcc ttcaaatcgt
    11881 caatcaccat cggcgtgagc ggactcagtg tcacctgcac gcccgaattg gcggcgatga
    11941 aagccagcac ctgggagccg aggtgaaagt gccgcaagat cagccagttg gcctgcggcg
    12001 acaggcagcg gttcatgccc caggccagga tgcggtgcag catcagcgag gcggcccact
    12061 tcttgggcat ggcgatcttg agcacctgga tcagcacgat gcaggctcgt gccagcggac
    12121 ggatgatggg cagcaggtat tgccgcgaac ccgaggccga gtcggccagc caggcgcgct
    12181 tgacggcgtc gtccagcggc gtgctctggt cgaggtagtt ggccagccac ggattggggt
    12241 cgcgcgggtc gtgcgcggcg tcgaaagggt cgggcttcat gggtcagatc aattcgaatt
    12301 gcagcgaata gagctgcgcc gtgtggcgcg cggtttgcac gatctgcgcc gcggcggcgg
    12361 gatggatggc gagcacgatg tccaccgcct gcagccagcg cgccagatgg tgctcgtcat
    12421 tggcgccgtg gtaggccaga aatcgaaacg cactcgcggg cagcttgacc tgttcgcgca
    12481 gcagcggcaa cagcgtgggc acgatgcgct ggccggtgcc ctcgatgatg tagatggcgc
    12541 ccagcaggcc cacggggttg gcctcggcgg ccagccggtg caggtaggcg ttgagcgcct
    12601 cgccgccggg gttgcgcgcc agctcatcca aggccagcgt gccgcccgca gcgcggtagt
    12661 cgtcatagag gatcttgaag tcgtcttgct cttcgcccgc gtgctggccg atcagcgcgg
    12721 ccagcggcag aaagcgggca tcgagctggg ccacggcttc gcgcatccag cggctgccct
    12781 cgcgcacctg tggtacccag tgctgcgtcc agcggcgata ggccgccacg tcaaaccgac
    12841 cgtgcaccag ttgctgcacg atgggcgtgc gccagacgcc cgagcggtag tcctgccaca
    12901 cgccggccag cgcggtgagc acggggcgca gcgccgcggg ggcgtcggcc ggcgcatgcg
    12961 gttcggccac ggcggcaggt gcgatggtgt cgtttgcttc tgtattgata gctgctcgcg
    13021 cttgttgggt gggcggaagc ggcatgtttt gcttgcaatc gaccacctcg aatgccatga
    13081 acgccgccgt catgcgcccc gactcgggca cgaagcacaa caccgtctcg cccgccttgg
    13141 gctgcacgct ggtaaaccat tcggccagca tgacgaagat ggaggcggcg cccgtgttgc
    13201 cgcgcgtggc caggttgctc caccacttgt cgtcgtcgat ggccagatcc gccttgtcga
    13261 gcatgtcgcg gatgaccggg atgaacttgg ccgacgagta gtggcagaga aagtggtcgg
    13321 tgcgcgccgg ctcgatccag ccttcgtgcg ccagcttcac gtattcgtga atgccaacgt
    13381 cgaacacgtg cggcagcagg cgaatgtcct gccgcagcgc cagcgcgccg gcggcttctg
    13441 cctcgctgga cgaggcgaag tcgaggaaag agcggctgcg gtcgtgcgtc aggcccagct
    13501 gcatgcacac cgggtagtcg cccgaaaagc tcttctgatg cagccacttg agtttgagcg
    13561 cgcgcccgct gtccccaatc ggctgcggcg tgcgacccag cagacaggcg ccggcgccgt
    13621 ccgacagcat ccagcgcagg aagtgggcgt cgaaatcggt ctggtagccg gcgggcgcga
    13681 agcgcgtgtg cttgaacatg cgcgagggca tgtcggcgcc caccgccagc gcgtgcgcgt
    13741 ggctgcccag cgccaccgcc tgcgcggcga actcgagcgc cgacacgccg gccacgcaca
    13801 cgccttgcga cgacagcgtc tgcatcggcg gcgcagccag ttcgccctgg atcatgttgg
    13861 caaagccggg catcagcgcg tcgccgcccg acgagccgac cgccagcagg gacacatcgc
    13921 ccagcgtggc gcgcgacgat tgaaggcagg cgcggatcgc gttggcggcc aggctggcgt
    13981 ggctttcgcg cgtgcggccg tcgggatcga tggcgtagtg gcgcgtctgg atgccgtttt
    14041 cggtcaggat gcgctgcttg atgcgcgccg acgcgcggtt cagcggcgcc acgtagtcgt
    14101 ccatcgcgtc gttgccgatc ggcgggccgg gcaggtaaag gcccaggccg ttgaggtgaa
    14161 cgttgtcaaa gcgaacgggc aatgggctct cctgaatcgc gctgtgaagg gggtgcggcc
    14221 tgcatcagcg aatggctgcg cgcgccgcgc catatcagcc cgccgaccag cgtgaccagc
    14281 gcgtagcgca gcagcgagcg ctctgtcagg cgcgggtcga tctgcgacgc gatgcgttgg
    14341 tgcgccgcgg gcagcgcgct ccagtgcagg cgcggctgca ggtggtgggc ggtgtggtag
    14401 ccgatgttga agaaccagcg gttcacgcca cccgtgaagt tgcgcgcaaa ctgcagtcgc
    14461 gaccgaccat cggcgtgcgc atgctgcagg tagttggcgc ccagcagcca gtgcaggccc
    14521 aacagctgcg gcagcagcac gaacttgagc cacgccgccg ggtcgatggc gatcatcagc
    14581 gcggtgaagg cgacggccca ggcgatctgc cgcaggtaat agcgccgcac cgccggcgcg
    14641 cgcgccgtca gccgcgccag ccaggcacca aacagcggcc acagcacgcc gacggcctgc
    14701 agcgggtgca gcaagtagcc ccacaggtgg ttggtgtcgc cgccggcaaa gcggtaggtg
    14761 cgcgcggcgt cgcgcgggcc gtggtggtag cggtgatggt tggcgttgtg cgcggggtga
    14821 aacagaaaca ccgggcggcc ctgcagcgcg ctgagcagcg tgtctgtcag ccggttcagg
    14881 cggtgcgtgc gccacaggcg caggtgcatg tggttgtgct cgatcaccgg cagcgccagg
    14941 ctgaggtaga tcagcaggcc aagcaccagc cctaaagccc agccgggcag ccgccccgtg
    15001 tacagcgccg ccacgcacag cggccacagc gcgatgcaca gcacgctgcg ccagtcgctc
    15061 gggtgtttga gccaacgcgc catggtcacg ggcgcgccgc gcggcgggcc agagccgcgt
    15121 caggcggcag attggtgccg aaccagcggt ccatgaacgg cagcatgaag ccgaaattgc
    15181 cgctgtagca ggcgtggtgc aggtggtggc gacgcgcgcc gttcaaccac cagcggtcgt
    15241 gcgcggcgcc cggcagaaag tcgtagttgg agtgaccgat gttgttgaac accaggctga
    15301 tgatgggcaa ggccagcagc gccggcagac tgaagtcgtg caacaccatg ggcagcagga
    15361 tcacgttgcc cagcagcacc gcctccagcg ggtgaaagct gtaggtggcc cagggcgtgg
    15421 tgaggaccga gcggtggtgc ggcaggtgaa agcgcttgaa ccagcgcgtg tgcagcagcc
    15481 ggtggttggc gtagaagtgc acttcgttcc acagtgtgag cgccagcacc tccagcgcga
    15541 tctgcagggc gccggcttgg tcgtcgatgc gtgcccaacc cagcttgagc agccaccacg
    15601 gcaccagcag cccggtgccg aagatgacaa tcgacagcgc cgacagccgc cattcgcgcg
    15661 ccagttgccc cgtcggcacg gcgcgcctgt ccagcaccga gccgtggccc agcatcggca
    15721 gcacgcgccg cgtgagccac agcgtgagcg cgcccagcag cagatagatg gcgctgaagt
    15781 acgccaggcc gaacgccacc acctggtacc aggtcaggtc aagaaggctc acgtgggtca
    15841 tgggaaggtg ctggggtgcg agcgcgattc aggccacatt gtccctgcgc tgtgccgtcg
    15901 cgcggtgggc gatgggcgag ggcgcatcgc acgcgccgga gggtagcatc cgtcgatacc
    15961 gggtcttttg ctgaatctgt gaatgctatg aatagaagag ctgttcgcgc tggataggca
    16021 agcgccagcg cctgttttta tctagatttc cgctggtgcg gcaaaacgct cgaacgtggt
    16081 ttgcaaccgc gccagccagc gcgccttgtc cgccgctggg gcaaaactgg cctcaaagct
    16141 gttcgctgcc aactgccagg cttgctcggc cgtcatgccg gtggcggcaa acacctgctg
    16201 aaagttggcg ttgatgtagc cgccaaagta ggccggatcg tctgaattga cggtggcgca
    16261 caggccagcg tccagcaggc gtttcaggtt gtgctgcgcc aggtcgggga acacgcgcag
    16321 cttctggttt gacagcgggc acacggtgag cggcacgcgc tcgcccgcca gtcgctgcat
    16381 caaggccgcg tcgtgcacgg cctgcacgcc gtggtcgatg cgttcggctt tcaatacgtc
    16441 gagcgcctgc cagatgtagg cgggcgggcc ttcttcgccc gcgtgggcga cgatgtgaaa
    16501 gcccagttcg cggcagcgcg cgaacacgcg ggcgaacttc tccggcgggt ggccgacttc
    16561 gctggaatcg agtccgacgc cgatgaagtg gtctcgaaac ggcagcgcgg cttcgagcgt
    16621 ctcagaggcc tcgcgctcgc tcaggtggcg caaaaagcac aggatcaact cggcgctggt
    16681 gccttgcgcc cgcgcatcgg cgcaggcgcg cgacaggccg cggatcacgg tctccatcgg
    16741 cacgccgcgc gcgctgtggg tctgcgggtc gaagaaaatt tctgcgtgca ctacgttttc
    16801 tgtagctgct cgcgcaagat aggcctgcgc cagatcgtaa aagtcttctt ccttcaacaa
    16861 cacgctggca ccggcgtagt aaatgtccag aaagctctgc aggtcggtga aggcgtaggc
    16921 gtggcgcagt tcttcgatgc tggcgtaggg cagggcaaca ccgttgcgtt cggccagcgc
    16981 gaagatcatc tcgggctcca gcgaaccttc gatgtgcatg tgcagctcgg ccttcggcat
    17041 ggcggcgagc agcgcgggca ggcggtcggg ggcgatggcg ggcagcttca tgcggccatt
    17101 gtgcgcgcaa caaaaagcgg ccccgatggg ccgcgattca tctgcaaggg caggcgatgc
    17161 cggtcagcgc agcaggccca gcatcagctt ggccagcgcc gcgcggccgc tgctttcgtt
    17221 ggcgaacacc gccaccccgt cgacggcacc gatggcgccc atgccgcggg tgatggggtt
    17281 gtagggcaac aggcccgggt tgttggcgcg ccaggccagc gtgccgccgc tgcgcgtgtc
    17341 gacgctgcca ttgcggtggt ggatggcgag cgtgttcgca tccggctgct ctacacgccc
    17401 gcgcgtggcc ggcagcggct tgaggtaggg gtttcgcggc ggctcgctgg tgcgcgattc
    17461 gccggtgttc cagcaagagc cgccgccaaa caggctcaac ccaccgctca tccagcctgt
    17521 catcaggaac aagcccagcg cctggccgaa ctgctgcgtg aattcgtagg gcttgtacgt
    17581 gccgggcttg gtgatgttga agtcctgctg cgtggccagc ttgccggtgt cgttgttgat
    17641 ccagccttcg ccgcaggcgt tttcgcgcac cacgaagccg tcggtggtgg cccagtccag
    17701 tgcttggccg ccgcggtcgg cgtactcgac tttcaggtcg cccaactcgt tctggctcag
    17761 gcccgtgatc ttcatcgcgt ggtcgccgcg cgtgacggtg acttcgctgg agacgtattc
    17821 attggggttg ttcttgaagg gctcggtgtc gatggtgatc ttggtgccgt cttccagctg
    17881 gaaggtggtc ttgccccaga agttggcgtc ggtcttgccg tccttgttcc agtcgatgtg
    17941 cgggtcgccc cagatgttgg tgacttcgcc gttgttcttg ttgacgatct tgatctgcga
    18001 gtttttctcg tccaattgca gctcgtactt gcccaaatcg atgttggccg tgccgttgcc
    18061 ggggtttttc accgtccact gcgcgtcggg cttggggcag aagcaaccct tcttgtcgac
    18121 cgaacccagc tggaagcggt tggtgtgggg cagcacgcag cgcggacgat cggcggcgcc
    18181 gatgggcgca aagccgacgg cggcaatggc gacaaaaccg gtttgggatg tggcgaacat
    18241 ggaaatcctc cgagagatag gtggtcggtt cgccgctgaa cgctgtggtg tgcagcggct
    18301 tgacacggag tagacccgtg accacgcccg ccaacaagct gttaccaatc ccatcggtaa
    18361 cgcccccatc ggggacgttc ccaggtcggg cgcgcgccgt tcaggcgatc acgccaaagc
    18421 cgatgccgct ttctttgaga tagcgccggt acgcggccga catgcggtcg gcggcggaat
    18481 gcacctcggc atccgggcgc agcggcaggc gcttgggccg ctgcgattca agcaccggct
    18541 ggtcctggcc gaagatggtg ttctgaaagt cgatcaggcg cgcgtcgggc gaatcgaagt
    18601 cgttcatcgc catgcgcgtc caggccacgc tgctttcctc cgtcaccggg cagacgaaca
    18661 tggcgatcga ttcccacagg ccctcgatgg ccgtgctgcc gggctcgggc ttcttggtca
    18721 gcaccgccat gtagggcgcg gtgacctcgt aggtgtattc gacctgggcg ccgctttcgg
    18781 cgtggatcga cgagcgcggc tgccacgcca ggcagccggt ggccagcacg ccgtgcggcg
    18841 tgggcgcgac ttcatacggc gggatctcgg gccggtcgcg gctgccgagc cagccttcgt
    18901 gcacgtagcc aaaatgcgcc atgtcgagga agttctcgat gatgcgcggc gcgctggcct
    18961 cgacccggta ggggccgctg gtggtcttgc gcaggcgcgg ctcgctttcg gcggcaaaca
    19021 gcggaaagtc gtggatcggc ggcgcgtcgg ccggcggtgg cgccagccgc acccacagca
    19081 tgccgtaggc ctcgcgcgcg tcgaagctgc gggcgcggtg gcgcgcgccg ggggtgaagt
    19141 cgggcagcgc cggaatgtgc gtgcactggc cgctgccctc gaaccgccag ccgtggtacg
    19201 ggcattccag ctggccgccc gccacgcggc cgagcgacag gcgggcgccg cggtgcgggc
    19261 actggtcggc ccaggcgtgg gccgcgccag cggcgtcgcg ccacagcacc aggtcttcgc
    19321 ccagcaggct ggcgccttgg ggttggctgc cgacggcgtg gctgagcgcc accgggtgcc
    19381 acagactgcg ttcgatcatg cgtgagatct gagtgaaaaa ggccgccagc gcttgatgga
    19441 caagcgcgag cagctattta ttttgaagca ggcaccttgc cttccacgcc cttgacgtag
    19501 aagtcgatct tgcccatcca gtcgtcgtcg gccttggtgc cggcggccag acgctccttg
    19561 ccgtcgccat cgaccaccgg gccggtgaag acgtcgaagc tgcgatcctt catcttggcc
    19621 ttgatctcgt cgaccttggc cttggcttcg gcgggaatgt cctcggccag cttgatgagg
    19681 tcgttctgcc cctcgggcac gccccagcgc gtgacctgac cgcctttcca ggtgccgtcg
    19741 atcacatcct tgagggtctt cttgtagtag tggctccagt cggcaatggc cgagcccagg
    19801 tgcgcgctgc cgccaaaggc gctcatgtcg ctgtcccagc cgaaggcgcg cttgccggcc
    19861 ttctcggccg cctgcagcac ggcggtggaa tcggtgtttt gcagcagcac gtcggcgccg
    19921 ccgttcatca gcgcctgcgc ggcttcggtt tccttgggcg ggtcgtacca ggtgttgacc
    19981 cacaccacgc gcaccttgat gccgggcttg acgctttgcg cgcccagcgt gaaggcgttg
    20041 atgttgcgca gcacctcggg gatcgggaag gagccgacga aacccagcgt gccggtcttc
    20101 gtcatgtgcg cggcgacgat gccggccagg tacgtgtcct gataaaagcg cgagtcgtac
    20161 acgttcatgt tgtcggccgt cttgtagccg gtggcgtgct cgaacttgac gtcagggaag
    20221 tccttggcga ctttcagcat cggctccatg aagccgaagc tggtgccgaa aatcaccttg
    20281 ttgccctgcg ccaccaggtc gcgcagcacg cgctcggtgt cggcgccttc gggcacgctt
    20341 tcgatgaagg tggtcttcag gcggtcgcca aattcggcct cggccgcctt gcggcccagg
    20401 tcgtgcgcgt agctccaacc ggccgagccg accgggccga cgtatatcca ggcggccttc
    20461 agcggctcgg cggccggggc gggcgcgggc ggcgcggcgg tttcggcgct ggcggccggc
    20521 gccgcaggcg cctcgtcctt cttgccacag ccggtgaggg cggcggcgcc cagggccgcg
    20581 gccgcggcgg tgagtcggag cagggagcgc cgggaagcaa agtgcgtcat cgaataagtc
    20641 ctgatcaggg tcggaaatgg aaaccgtgcc cgtgcaacaa gcgtgccggc ccatgttacc
    20701 gatttggtgc ggcgattctg gcgcgccaaa aaaaaagggc gcccaagcac cctgttttca
    20761 gccaccgcct caatcgccgc caccgccctt ccagcggccg ccgtgtccgg acctgcgccg
    20821 ggcactggcg gcgtcccccc tcccgcagga gaggggggaa ggcgcgtcag cgcctcaggg
    20881 ggtgtcactt cttgtcacca ggcaccttgc cctcgacccc cttgacgaag aagttgatgc
    20941 cgcccaggaa tttgtcgtcg gccacggcgt ccttggccag cacttccttg ccggtgttgt
    21001 cgctgatcgg acccttccag atgttgtagc tgcccgcctt caggccggcc ttcacgtcgt
    21061 cgagcgcctt cttggcttct tccggcacgt cggcggcgag cgacaccagg tcgatcgcgc
    21121 cttccttcac gccccaccag gtctggccgg tggcccactt gccatccagc acctcgccca
    21181 cggtcttgat gtagtagggc gcccagttga tgatggccga gcccaggtgc gccttggggc
    21241 cgtacgcggt catgtcggaa tcccagccga agccgcgctt gcccatcttc tcggcggttt
    21301 gcagcacggc cgacgagtcg gtgttctgga acaggatgtc ggcgccgccg ttgatcagcg
    21361 ccgtggcggc ttcggtctct ttcggcgggt tgaaccactc gttgacccac accaccttgg
    21421 tcttgatgtt ggggttgctg ctttgcgcgc ccagcgtgaa gctgttgatg ttgcggatca
    21481 cttccgggat cggaatcgac gccaccacgc ccagcgtgtt cgacttggtc atcctgccgg
    21541 cgatcacgcc cgccatgtag gcgccttcat acgtgcggct gtcgtaggtg cgcatgttgg
    21601 gcgcggtctt gtagccggtg gcgtgctcga acttcacctc ggggttgtcg gcggcgactt
    21661 tcagcatcgg ctccatgtag ccgaaggtgg tgccgaagat cagcttgttg ccctggccgg
    21721 ccatgtcgcg gatcacgcgc tcggcgtcgg ccgattcggg cacgctctcg acaaagctgg
    21781 tgacgatctt gtcgccgtac tccttctcca gcgccttgcg cgcgttgtcg tgcgcaaaag
    21841 tccagccgcc gtcgcccacc gggccgacgt aggcaaaggc gatcttcagc ggatcggcct
    21901 tggccggcgc gggcgcagat gcagccgccg gtgccggcgc gggcgcagcg gcggtgggcg
    21961 ccggctcgtc tttcttgccg caggccacca gcgcgctggc ggcggcggcc agtgcggcga
    22021 gtttcaggat cgaacgcttg tgttggtcgg tcattcggtg gctccttgcc ctggtgtgtg
    22081 gatgaaatgc agattatggc ggcgcccgag cagcgcccga aaattcaagc caaatgggcc
    22141 gccagccctt gaccgacaag cgcgaggcgc tatccaaaat atagtattca ggcgcatcaa
    22201 gcgcccggat gaaacggctt gcccagcgag gccggcatgt tgacgcggat ccacgtcggg
    22261 ttgcgcg
//
LOCUS       DAOMVO010000009        22141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_392_length_22141_cov_17.847279, whole genome shotgun sequence.
ACCESSION   DAOMVO010000009 DAOMVO010000000
VERSION     DAOMVO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 22141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22141
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_392_length_22141_cov_17.847279"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="PKV30_01070"
     CDS             complement(<1..1644)
                     /locus_tag="PKV30_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRL28842.1"
                     /translation="MTALELTLLYLVAAVLGVAACRWLKLPAILGYLAAGVLMEPHTL
                     GLAFGQDSESIKYLAEFGVVFLMFVIGLEFNLPKLRAMRRHVFGLGLAQVVLTMLIVT
                     GFLLALAALMPARWPISWQTALALSGALAMSSTAIVVKMMAERLELDSEHGQRVMGVL
                     LLQDLAVVPLLVLIPALGAPPEQLLTALVWAGVKATALIALLLWGGQRVMRWWLTLVA
                     RRKSQELFVLNLLLMALGLAWLTEHAGLSLALGAFICGVLISETEYRVQVEAEIRPFH
                     DVLLGLFFITIGMLLDWRLVAVQWPLVLALLVVPVLFKLVLVAALAKGLGASSGVALR
                     TGLYLAQAGEFGFVLLTLSRREGLLAPELFNPILAAMVLSMLATPFIIMASNRIVMRL
                     VSSEWMMQSLQMTSIARQSINTRGHVLICGYGRSGQAMARVLTQEGVPYIALDLDPDR
                     VRQAQAAGQAVAFGDAAKPQALIAAGLNRASAVAITYLDTHSVLQVLTTIRAHAPQLP
                     IIVRTQTDRELERLRAAGATEVVPESHEGSLMLAGHALAL"
     gene            1778..2755
                     /locus_tag="PKV30_01075"
     CDS             1778..2755
                     /locus_tag="PKV30_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRL28843.1"
                     /translation="MSFDPAQALRLAHDTLDIEAAAVLGLKRRLGDDFARAVALMLDV
                     AGRVVVMGMGKSGHVGRKIAATLASTGTPAMFVHPAEASHGDLGMIKAVDVVLAISNS
                     GESDELAAIVPLVKRLGAPLVAMTGQPRSTLARHADVVLDSGVEKEACPLNLAPTAST
                     TAQLALGDALAVALLDARGFKAEDFARSHPGGALGRRLLTRVSDIMRSGDAVPQVPPE
                     ADFSRLMHEMTRKGLGASAIADADGRALGIFTDGDLRRLVETGRDLRALTAREVMHAA
                     PRTIADDALAAEAAEAMEKFRVNVLLVTDADDRLVGALNANDLMRAKVI"
     gene            2755..3309
                     /locus_tag="PKV30_01080"
     CDS             2755..3309
                     /locus_tag="PKV30_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRL28844.1"
                     /translation="MAPALHFPPELLLRAQGIRVVFFDVDGVLTDGGLYFSAEGETLK
                     RFHTLDGHGIKLLAQAGITPCVISGRDSAPLRVRLKALGVQRLRLGTEDKLPAAQAFL
                     DELGLDWSQAAAMGDDWPDLPVMQRAAFACAPPQAHAEARALATHITTARAGEGAARE
                     LCDLLLVAGGHYRRLLDAHAGAAE"
     gene            3306..3935
                     /gene="lptC"
                     /locus_tag="PKV30_01085"
     CDS             3306..3935
                     /gene="lptC"
                     /locus_tag="PKV30_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRL28845.1"
                     /translation="MNAARLLRYGRDQLSAWLPALLMMLFALGTWWLVRSAPKAVSQN
                     EGQVASAEPDYFMRQFSVRSFEPAGRLKSEIFGTEGHHLPANDTLEVQEPRILSFNEQ
                     GHPTVATARRALAKGDGSEVRLYQDARVVREPLAKTGKAEATPRLEFQGEFLHVFVDE
                     ERVSSDQPVRLMRGADVFTGDVFAYDDRSGVAELSGRVRGVLQPKKGAP"
     gene            4058..4837
                     /locus_tag="PKV30_01090"
     CDS             4058..4837
                     /locus_tag="PKV30_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL28846.1"
                     /translation="MTAPLVFITGASSGIGQALAARYAAAGWRLALVARRGAEMTAWA
                     QAQGWDAQRWRVYPADVAEVESIVAAGRACLAAQGVPDVVIAGAGISIGMDTAERSDL
                     DVMARTFAVNNTGMAATFHPFIAPMRQRGSGTLVGIASVAAIRGLPGHGAYCASKAGV
                     VSYCESLRGELRGSGVRVVTLGPGYIDTPLTRRNTYSMPFLMSAEAFAERAFTAIARG
                     VSYRVIPWQMGVVAKLLRVLPNALFDRLLSGRARKARQGEG"
     gene            complement(4961..5554)
                     /locus_tag="PKV30_01095"
     CDS             complement(4961..5554)
                     /locus_tag="PKV30_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRL28847.1"
                     /translation="MGNKLYVGNLPYGVRDADLEQAFSQFGSVTSAKVMMERDTGRSK
                     GFGFVEMGSDAEAQEAINGMNGQSLGGRNVVVNEARPMESRPPRTGGYGGGGGGGYGG
                     GGGGYGGGGRREGGGGYGGGGGGGGGYGGGGGGGYGGGRREGAGGGGNDGNFRSPYGA
                     GPRGGGGGRREGGGGYGGGRREGSYDGGGRDGGGDDY"
     gene            5934..6503
                     /locus_tag="PKV30_01100"
     CDS             5934..6503
                     /locus_tag="PKV30_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HRL28848.1"
                     /translation="MEAFLISTGIVALAEMGDKTQLLSLVLAARFRKPWPIVLGIFVA
                     TLANHALAGAVGSWVTRMLGPDVLRWVLGASFIAMAIWMLIPDKLDEDDQPSTTSKLG
                     VFGTTLVAFFLAEMGDKTQIATVALAAQYQQWAAVVAGTTLGMMLANAPVVWFGDRLV
                     KRVPIRVVHVISALIFAVLGVVALLGVGT"
     gene            6633..7775
                     /locus_tag="PKV30_01105"
     CDS             6633..7775
                     /locus_tag="PKV30_01105"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRL28849.1"
                     /translation="MSFIATPQSLHFDAPLPLQSGASIRGYDLTYETYGELNAARSNA
                     VLICHALNASHHVAGVYEAQARSEGWWDNMVGPGKPVDTHRFFVIGVNNLGSCFGSTG
                     PMHVNPETGQVYGADFPVVTVEDWVNAQARLLDALGIEQLAAVMGGSLGGMQALSWTL
                     QYPERVRHAVVVASAPNLTAENIAFNEVARRAIVTDPDFHGGHFYAHGVVPARGLRIA
                     RMIGHITYLSDDVMNEKFGRELREAVAGNDSGYRFSTQDVEFQIESYLRYQGDKFSEY
                     FDANTYLLITRALDYFDPARAFGGDLTQALARTRAKFLLVSFTTDWRFAPARSREIVK
                     ALLENRREVSYAEIDAPHGHDAFLLDDARYMGVVRSYFESIAEVQA"
     gene            7772..8356
                     /gene="metW"
                     /locus_tag="PKV30_01110"
     CDS             7772..8356
                     /gene="metW"
                     /locus_tag="PKV30_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRL28850.1"
                     /translation="MSDAQVQHTIAQLVPEGAHVLDLGCGDGALLQHLITTRRCTGYG
                     IEIDDDNVLACVRRGVNVLQLNLHEGLKAFRDQSFDAVLQIDTLQHLRNAEVMLRETA
                     RVGRVGIVAFPNFAHWPNRLSIARGRMPVTRRLPYQWYDTPNIRVGTFKDFEALARKN
                     GLQILDAFGLEDGRVVRWLPNARAGTAVFKLQRA"
     gene            8439..9794
                     /locus_tag="PKV30_01115"
     CDS             8439..9794
                     /locus_tag="PKV30_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRL28851.1"
                     /translation="MYPTIDQLAADLAAGSTTSLQLTEAALARAQDPAGEGARVFTLI
                     DAARARAAAQASDLLRAAGMVRSPLEGLPVSVKDLFDVAGQVTTAGSVVLKDAPPAER
                     NAPVIDRLIAAGAVIVGRTNMTEFAFSGLGLNPHYGTPKNPWDRAGGGRIPGGSSSGA
                     AVSVTDGMAVVGIGSDTGGSVRIPSALCGLTGFKPTQRRVPIDGTVPLSTSLDSIGPL
                     AASVRCCAIADAVLAGEPPVVPAARDVRDARLAVPETLVLDSMDSTVAAAFERACARL
                     QAAGARLSRIAVPEFAELGRLHARGTLAGAEAWAWHRTLLTTRGDEYDPRVGLRMRGG
                     EAMGAADYIDLLAARRRWIAQVEARIAGFDALLMPTVPIVAPRIDALTNSDEAYFAAN
                     GLMLRNPTLINFLDGCALSLPCQAEGEPPVGLTLAGAGGRDAEVLALGLAVEDVLRSA
                     R"
     gene            complement(9905..10597)
                     /locus_tag="PKV30_01120"
     CDS             complement(9905..10597)
                     /locus_tag="PKV30_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRL28852.1"
                     /translation="MSDTPTPQHLQATPLIDAGHPDVQAFAREHAQGAGERARAVALV
                     HAVRDGFRYDPYRIDLSPNGMRASTVLANGYGWCVPKATLLTAVARAAGIPARLGFAD
                     VRNHMSTERMRETMKTDLFIWHGYTELWLEGAWRKATPAFNIELCEKFGLLPLDFDGV
                     HDSIYHAFDKAGNRHMEYVNERGAFDDLPLDRLVANFHATYPGWVPGDVLQSELTQGD
                     FLADVARETGRA"
     gene            10678..12030
                     /gene="chrA"
                     /locus_tag="PKV30_01125"
     CDS             10678..12030
                     /gene="chrA"
                     /locus_tag="PKV30_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRL28853.1"
                     /translation="MPTAVPFGAALRFWLNLGCISFGGPAGQIAIMHRELVEQRRWIS
                     ERRFLHALNFCMLLPGPEAQQLATYIGWLLHGTRGGIVAGALFVLPSLVLLIVLSAAY
                     LAWGQLPWVAALFHGIKAAVAAIVLHAMWRLGGKTLTTPLAWALAGGSFVALAWWKWP
                     FPAVVLGAGAVGWLAARVAPGQLVPPVAAPSAAAESAAVMGDDTPAPPHAHFSRHGLA
                     RVLLAGALAWLLSMGGLTAWQGLDATLTQVGWFFSKAALITFGGAYAVLPYVNQAAVQ
                     QHQWLTAAQMMDGLALGETTPGPLIMIVAFVGQLAATAEAMRACGVNGCGAWALGWGA
                     LGALVATWFTFLPSFVFILAGGPLVESTRGKLGFTASLAAITAAVVGVIGSLALFFVA
                     NVALPSGAGAGWDSKIDWAALALVAAATLALVRLKWGVIRVIALCALAGGAWRWFGVG
                     "
     gene            complement(12040..12699)
                     /locus_tag="PKV30_01130"
     CDS             complement(12040..12699)
                     /locus_tag="PKV30_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRL28854.1"
                     /translation="MKFEVILFDCDGVLVDSEPITHGVLRDLLAESGWDMPLAECMRH
                     FIGRTVRSQAALIEARTSQPLTDEWMATFYARRNAELEARLQPIAGALEAVRAAHDMT
                     GGRIAVASGADRGKVLLQLAKVGLAEWFGAHVFSGHDLPRSKPFPDVYLAAAQSLGVA
                     PARCLVIEDTPTGIEAGVAAGATVWAYCPQAEQANALRAAGAHEVFASMAELPARWAM
                     R"
     gene            complement(12863..14323)
                     /locus_tag="PKV30_01135"
     CDS             complement(12863..14323)
                     /locus_tag="PKV30_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRL28855.1"
                     /translation="MKRFDAAFLVLSWVVMGFSFAFFVPMAWDWFGEGGDHVRVWGWC
                     FLFTLATGGWLWVRTRHAHRELTVRDGFLLVTLVWVVMPAYASLPLLFTVQGISFTDA
                     YFEAVSGLTATGATALAGLDLLAPSVNIWRCFLQLIGGLGIMLLAVAILPFLGLGGAQ
                     LYKAEMPGPMKEQRLTPRLAETARGLWGVYFVLSLGCFLAYRAGGMTWSDAFMHMCTT
                     VGLGGMSSHDQSFGYWNAPHLEWAAVLFMGLSGISFARYFMLWRQRSLSPVLGDTEVR
                     AYIGTLLLCTLLVCAMLLMEHVIDDFPSAFRQAAFQVVSVATTTGYATTNYLLWPSFV
                     PVLLLFLGCFVSCAGSTGGGIKMVRVLVLVKIAQRELVRIIHPRVVYPVSLGRTAVPP
                     DVIWAVMAYMLIYGGTMIGMTLLMLASGLDVVTAFTAIVACLNNIGPGLGKVGPAGNY
                     GVLNDFQIWLCSAAMLLGRLELLSVMVLFTPQFWRR"
     gene            complement(14439..15827)
                     /gene="trkA"
                     /locus_tag="PKV30_01140"
     CDS             complement(14439..15827)
                     /gene="trkA"
                     /locus_tag="PKV30_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRL28856.1"
                     /translation="MRIIILGAGRVGESVAETLVSEANDITVVDPDPERLRALEDRLD
                     LRGVAGNGVQPSILRAAGADDCDLFIACTPLDETNLVACKIAHDVFGIPTTIARLRSP
                     EFSQGDKLLDKDGFSVDYVICPEESVTRTIHQLIDYPEALQVVKFSQSQAHLVVVRVS
                     AGSYLADHSIAEFRAHKADVPMRIVAIYRGDEELPVQAETRVRAGDEVFVLTGKESVR
                     RALHAIGNPDRPVRRMMIAGGGKVGLRLARGLVGQCSVKIIEANRKRCDYLASELPSE
                     MLVLQGDGTDEELLVDENVADMDLFIAVTSDDEDNILAAMLAKRLGARRVIALINRRA
                     YADMLQGSTIDIAISPAHAVIGELLMHVRRGAVAAVHSLRGGTAEALEGVAHGDAKTS
                     HLVGRPIEQIKLPAGARIGAIVRGEGEGARTVVPTHDTVIETDDHVIIFLPHKRMVRE
                     VEKLFQVRATFL"
     gene            complement(15942..16364)
                     /locus_tag="PKV30_01145"
     CDS             complement(15942..16364)
                     /locus_tag="PKV30_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HRL28857.1"
                     /translation="MTPAAAACPCGRLDARRRPLDWAACCGRYIDHFSDTPAPDAESL
                     MRSRYSAFVCERAEYLLATWHPSKRPATLDFDTGTQWLGLEVRARRRLDDAHAEVEFV
                     ARSRLHGRATRLHEQSCFVRENGANGVARWFYVDGTLL"
     gene            complement(16373..16792)
                     /locus_tag="PKV30_01150"
     CDS             complement(16373..16792)
                     /locus_tag="PKV30_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL28858.1"
                     /translation="MPITKGFRALVDEAMAQVTTHSVDQVRQRLDDPALQLVDIRDAR
                     ELEREGTVPGVINAPRGMLEFWVDPDSPYYKPVFGDEDKQYVLFCGAGWRSALAAKAL
                     QDMGMTNVAHIDGGFTEWAKQGAPVESYEAHRARRAK"
     gene            complement(16804..17940)
                     /locus_tag="PKV30_01155"
     CDS             complement(16804..17940)
                     /locus_tag="PKV30_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL28859.1"
                     /translation="MLLTQDQEMIRDAVRSFAQEQLWPHAAQWDKEHAFPEAAHKGLA
                     ELGAYGICVPEEYGGAGLDYITLALVLEEVAAGDGGTSTPISVTNCPYNAILMKYGTE
                     AQKQKWLAPAARGEMLGAFALTEPHVGSDASSLRTTAVKDGDEYVLNGVKQFITSGKN
                     GHAAVVIAVTDKAAGKKGMSAFIVPTHRLGNHGWVVARIEDKLGQHSSDTAQINLENC
                     RVPAENLIGEEGGGYKIALSALEGGRIGIAAQSVGMARSALEVAIQYAKERESFGTAI
                     FNHQAVGFRLADCATRLEAARQLIWHAASLRDAGLPCLKEAAMAKLFATETAEAVCSA
                     AIQTLGGYGYVSDFPVERIYRDVRVCQIYEGTSDVQKIIITRAL"
     gene            complement(17945..18610)
                     /locus_tag="PKV30_01160"
     CDS             complement(17945..18610)
                     /locus_tag="PKV30_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL28860.1"
                     /translation="MSTVLIIGASRGIGWELARQYAEAGDRVIATARDDAALARLRSI
                     GAEPLRVDVADAASISGLAWQLDGEKIDTALYVAGVTSHGDATEPPTREAFDTVMHAN
                     VLGAMQAIPQVAPLLAPGGRFGFVSSVMGRIATVDNSRAWVYRTSKAALNMAVASAQS
                     AWPNAIMVCLHPGWVQTDMGGGAAPVTPEASASGLRRVMAALTPADRGAYIQYDGQRF
                     DTW"
     gene            complement(18618..19262)
                     /locus_tag="PKV30_01165"
     CDS             complement(18618..19262)
                     /locus_tag="PKV30_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRL28861.1"
                     /translation="MIDSADTLRRLYPQASPRSRAKQLDRLDDTMRRFVAHAPLCVIA
                     SAGAAGEGLDSSPRGGRPGFVQVADERTLLIPDHTGNNRLDTLENLLADPRIGLLFLI
                     PGVNEVLRVNGTAQLRDEPEFTQRFAHADAPKLPRLVIEVAVREAYLHCPKALLRSQV
                     WSPEARVAAGSFPSMNAMLLAQLGPQVVTETDDEALARYRAQLADEGLAHLVKG"
     gene            complement(19337..20518)
                     /locus_tag="PKV30_01170"
     CDS             complement(19337..20518)
                     /locus_tag="PKV30_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRL28862.1"
                     /translation="MSDPIVIVSAARTPMGSFQGDFTPLAAHDLGGAAIKAAVERAGI
                     QPELVTEVIFGNCLMAGQGQAPARQAAFKGGLPKSAGAVTLSKMCGSGMRAAMFAHDM
                     LVAGTHDVLVAGGMESMTNAPYLLQKGRGGYRMGHDKVYDHMMLDGLEDAYEAGRSMG
                     TFGEDCAAKYNFTRDAQDKFAMASVRRAQDAIKNGAFKDEIAPVTVKDRKGERVVDTD
                     EGPGKINIDKIPTLKPAFKKDGTVTAAASSSINDGAAAMVMMRESTAKKLGVKPLARI
                     VSHATHAQEPEWFSTAPIGATEKALEKAGWKVDDVDLWEINEAFAVVPMALMHDLKVP
                     HDKVNVNGGACALGHPIGASGARIMVTLIHALKARGKKKGLATLCIGGGEATALALEL
                     V"
     gene            complement(20561..20941)
                     /locus_tag="PKV30_01175"
     CDS             complement(20561..20941)
                     /locus_tag="PKV30_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HRL28863.1"
                     /translation="MSGVLVDTSVWVDHFRRRNDALIGLLGLDQVLGHPLVVGEVACG
                     TPPERHQTLADLALLQPAQQASVDEALQFIENEHLFGLGCGLVDIMLLASTLMTPGAR
                     LWTLDHRLAGLADRFAVLHRPQVH"
     gene            complement(20938..21141)
                     /locus_tag="PKV30_01180"
     CDS             complement(20938..21141)
                     /locus_tag="PKV30_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRL28864.1"
                     /translation="MRTTVTIDDALYEKALEVADPDMDKADLFREAIKTFVRVQAARR
                     LAALGAAAPRMSDVPRRKGAASA"
     gene            complement(21251..>22141)
                     /locus_tag="PKV30_01185"
     CDS             complement(21251..>22141)
                     /locus_tag="PKV30_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRL28865.1"
                     /translation="LPSFPYVFKVIKDWYPPPKETTREQIRGKYQLVKQHDRVGRMAD
                     TMEFSLLAFPRDRFDDDLIAEIEKFAPSQLEISDRDGDGQIEVIISHAYIERRMIPLN
                     LYLQEAFDAGDDPAAHAQMEHAVIEYGNAIKDLVAANIFPGDMLWKNFGVTRHGKVVF
                     YDYDEIEYITDTNFRRVPPPRNEEDEMSGEIWYSVAKHDVFPETFGPFLLGNPRVREA
                     FMRHHADLLEPEFWQQTKARIQQGEVIDFFPYGVHRRFEVNGGVTGLSELPDPAAFAV
                     ETPADQPDTSAPVTSAESSE"
BASE COUNT         3484 a         7445 c         7499 g         3713 t
ORIGIN      
        1 cagcgccagc gcgtggccgg ccagcatcag cgagccttcg tgcgattcgg gcaccacctc
       61 ggtggcgccg gcggcgcgca ggcgttccag ctcgcggtcg gtctgcgttc gcacgatgat
      121 gggcagctgc ggcgcgtggg cgcggatggt ggtcagcacc tgcagcacgc tgtgcgtgtc
      181 caggtaggtg atggcgacgg cgctggcgcg gttcaggccg gcggcaatca gcgcctgcgg
      241 cttggcggcg tcgccaaagg ccaccgcctg gccggcggcc tgcgcctggc gcacgcggtc
      301 ggggtcgagg tcgagcgcga tgtagggcac gccttcctgc gtcagcacgc gcgccatggc
      361 ctggccgctg cgcccgtagc cgcaaatcag cacgtggccg cgcgtgttga tcgactggcg
      421 agcgatgctg gtcatctgca gcgactgcat catccactcg ctgctcacca gccgcatcac
      481 gatgcggttg ctggccatga tgatgaaggg cgtggccagc atcgacagca ccatcgccgc
      541 caggatgggg ttgaacagct cgggcgccaa aaggccctcg cgccggctca gggtgagcaa
      601 cacaaaaccg aactcgcccg cctgcgcgag gtacaagccg gtgcgcagcg ccacgccgct
      661 gctggcgccc agccccttgg ccagcgcggc caccagcacc agcttgaaca gcaccggcac
      721 caccagcagc gccagcacca gcggccattg cacggccacc aggcgccagt cgagcagcat
      781 gccgatggtg atgaagaaca gacccagcag cacgtcgtga aaggggcgga tctcggcctc
      841 gacctgcacg cggtattcgg tttctgaaat cagcacgccg cagatgaagg cgcccagcgc
      901 caggctgagg cccgcgtgct cggtcagcca ggccaggccc aatgccatca gcagcaggtt
      961 gagcacgaac agctcctggc tcttgcgccg cgccaccagc gtcagccacc agcgcatcac
     1021 gcgctggccg ccccacagca gcagcgcgat cagcgccgtc gccttgacgc cggcccacac
     1081 cagtgccgtc agcagctgct cgggcggcgc gcccagcgcg gggatcagca ccaaaagcgg
     1141 caccaccgcc aggtcctgca gcagcagcac gcccatcacg cgctggccgt gctctgaatc
     1201 cagctccagc cgctcggcca tcatcttgac gacgatggcg gtgctgctca tggccagcgc
     1261 gccggacagc gccagcgcgg tctgccaact gatcggccag cgcgcgggca tcaaggccgc
     1321 cagcgccagc agaaagccgg tgacgatcag catcgtcagc accacctgcg ccagccccag
     1381 gccgaacacg tggcgccgca tggcgcgcag cttgggcagg ttgaattcaa gcccgatgac
     1441 gaacatcaag aagacgacgc caaattcggc caggtacttg atgctttccg aatcctgccc
     1501 gaaggccagg cccagcgtgt gcggctccat cagcacgccg gccgccagat aacccagaat
     1561 ggccggcagc ttgagccagc ggcacgccgc cacgcccagc acggcggcca ccaggtacag
     1621 cagcgtgagt tcgagggcgg tcatgcgggt ggtgggcgaa gggtgtcgat gattatgcta
     1681 ctcctgaaaa tatagctgcc tgcgcttgat ggacgtgcgc cagcggcatt tttgactcat
     1741 aaatcatgcg caggcgcctg ccgataaaat gccccgcatg agtttcgacc ccgcccaggc
     1801 gctgcgcctg gcccacgaca cgctggacat cgaagccgcc gccgtgctgg gcctcaagcg
     1861 ccgactgggc gacgactttg cgcgcgcggt ggcgctgatg ctcgacgtcg ccggccgcgt
     1921 ggtggtgatg ggcatgggca agagcggcca cgtcggccgc aagatcgccg ccacgctggc
     1981 gtccacgggc acgccggcga tgttcgtgca cccggccgaa gccagccacg gcgatctggg
     2041 catgatcaag gcggtcgacg tggtgctggc catttccaac agcggcgaaa gcgacgagct
     2101 ggccgccatc gtgccgctgg tcaagcgcct gggcgcgccg ctggtggcga tgacgggcca
     2161 gccgcgctcc acgctggcgc gccacgccga tgtggtgctc gacagcggcg tcgagaaaga
     2221 agcctgcccg ctcaacctgg cgcccaccgc cagcaccacc gcgcaactgg cgctgggcga
     2281 tgcgctggcc gtggcgctgc tggatgcgcg cggattcaag gccgaggact ttgcccgctc
     2341 gcaccccggt ggcgcattgg gccgccgctt gctgacgcgc gtgagcgaca tcatgcgcag
     2401 cggcgacgcc gtgccgcagg tgcctccgga ggccgacttc agccgcctga tgcacgagat
     2461 gacgcgcaag ggcctgggtg cctcggccat cgccgatgcc gacggtcgcg cgctgggcat
     2521 cttcaccgat ggcgacttgc gccggctggt ggagaccggg cgcgacctgc gcgcgctgac
     2581 ggcgcgcgag gtgatgcacg ccgcgccgcg taccattgcc gacgacgcgt tggccgccga
     2641 ggcggccgag gcgatggaaa aatttcgcgt caacgtgctg ctggtgaccg acgccgatga
     2701 tcgtctggtg ggcgcgctga acgccaacga cttgatgcgg gccaaggtga tctgatggcg
     2761 ccggcgttgc actttccgcc cgagttgctg ctgcgcgcgc agggcattcg cgtggtgttt
     2821 ttcgatgtcg atggcgtgct gaccgatggc ggcctgtact tcagcgccga gggcgagacg
     2881 ctcaagcgct ttcacacgct ggacgggcac ggcatcaagc tgctggcgca ggccggcatc
     2941 acgccctgcg tgatttcggg gcgcgattcg gcgccgctgc gcgtgcgctt gaaggcgctg
     3001 ggtgttcagc gcctgcgcct gggcaccgag gacaagctcc cggccgcaca agcctttctc
     3061 gacgaactgg gcctggactg gtcgcaagcc gccgccatgg gcgacgactg gcccgacctg
     3121 cccgtgatgc agcgcgccgc ctttgcctgc gcgccgccgc aggcgcacgc cgaggcgcgc
     3181 gcgctggcga cgcacatcac caccgcgcgc gcgggcgaag gcgcggcgcg cgaattgtgc
     3241 gacctgctgc tggtggccgg cggccattac cggcgcctgc tcgatgcgca tgcaggggcc
     3301 gccgaatgaa cgctgcgcgc ctgctgcgct atgggcgcga tcagctgtcg gcctggctgc
     3361 cggccttgct gatgatgctg ttcgcccttg gcacctggtg gctggtgcgc agcgcgccca
     3421 aggccgtgtc gcagaacgag gggcaggtgg cgtcggccga gccggactac ttcatgcgcc
     3481 agttctcggt gcgcagcttt gagccggccg ggcggttgaa gtccgaaatc tttggcaccg
     3541 aaggccatca cctgccggcc aacgacacgc tggaggtgca agagccgcgc atcctgtcgt
     3601 tcaacgaaca gggccatccc accgtggcca cggcgcggcg cgcgctggcc aagggcgacg
     3661 gctcggaagt gcgcttgtac caggacgccc gcgtggtgcg cgagccgctg gccaagacgg
     3721 gcaaggccga ggcgacgccg cgtctggagt ttcagggcga gtttttgcac gtcttcgtgg
     3781 atgaagaacg cgtcagctca gaccagccgg tgcggctgat gcgcggcgcc gatgtcttta
     3841 ccggcgacgt gtttgcctac gacgaccgca gcggcgtggc cgagctttcc ggccgggtgc
     3901 gtggcgtact acagccgaaa aaaggcgccc cctgagtcgc ctgcggcgcc tttcctccga
     3961 gggggcaacg ctggtggccg gggaggcccc tgccacggcg ttcacgaatg gcctgctccc
     4021 cggccttttc tctttttgaa tgtctgatcg tcaacccatg accgcaccct tggttttcat
     4081 caccggcgcc tccagcggca tcggccaggc gctggcggcg cgttacgccg ccgctggctg
     4141 gcgcctggcc ctggtggcgc ggcgtggcgc agagatgacg gcctgggccc aggcgcaggg
     4201 ctgggatgcc cagcgctggc gcgtctaccc ggccgacgtg gcggaggtcg aatccattgt
     4261 cgcggccggg cgtgcctgtc tggcggccca gggcgtgccg gacgtggtga tcgcgggcgc
     4321 cggcatcagc atcggcatgg acacggccga gaggagcgat cttgacgtga tggcgcgcac
     4381 cttcgccgtc aacaacaccg gcatggcggc cacctttcac cccttcatcg cgccgatgcg
     4441 ccagcgcgga agcggcaccc tggtcggcat cgccagcgtg gcggcgattc gcgggctgcc
     4501 gggccacggt gcgtactgcg ccagcaaggc cggcgtggtg agctattgcg aaagcctgcg
     4561 cggcgagctg cgcggcagcg gcgtgcgcgt ggtcacgctg gggccggggt atatcgatac
     4621 gccgctgacg cgccgcaaca cctattcgat gccgtttctg atgtcggccg aggcgtttgc
     4681 cgagcgcgcc ttcaccgcca ttgcgcgcgg cgtgagctac cgggtgattc cgtggcagat
     4741 gggcgtggtg gccaagctgc tgcgcgtgct gcccaatgcg ctgttcgacc gtttgctgag
     4801 cggccgcgcg cgcaaggcgc gccagggcga gggctgattc gcccgctgcc gtgggcacgc
     4861 accatgaaaa aaggcgccac accgggcgcc ctttgtttgc gtgggcaccg gatgagctca
     4921 tccgggccga ctgccaccgc catgcgcggt ggcttaaaaa tcaatagtcg tcgccgccgc
     4981 cgtcgcggcc gccgccgtcg tagctgcctt cacggcgacc accgccgtag ccgccaccgc
     5041 cttcacggcg accaccaccg ccgccacgcg ggccggcgcc gtaggggctg cggaagttgc
     5101 cgtcgttgcc accgccgccg gcgccttcac ggcgaccgcc gccgtagcca ccgccgccac
     5161 cgccgccgta accaccacca ccaccgccgc cgccgccata gccgccaccg ccttcacggc
     5221 gaccgccacc accgtagccg ccgccaccgc cgccgtaacc accgccgccg ccaccaccgt
     5281 aaccaccggt gcgcggggga cgcgattcca tcggacgtgc ctcgttgacg accacgttgc
     5341 ggccgccgag cgactggccg ttcatgccgt tgatggcttc ctgcgcctcg gcgtcactgc
     5401 ccatctcgac aaagccgaag cccttggagc ggccggtgtc gcgctccatc atcaccttgg
     5461 cgctggtcac cgagccgaat tgcgaaaacg cctgctccag atcggcgtcg cgcacgccgt
     5521 acggcaggtt accgacgtaa agtttgttgc ccattaaaag ggactcctca agaacactgt
     5581 aaaaaagcga tggagtccgc aaaacgcctt caacgaacct tggctcggcg aagaagggca
     5641 actgactgtg ttcaccgcaa aaaccacgca tgccttcaag gcatacccgc acaattatgc
     5701 gccagaacct tgaaagtcaa gcatgcggat cttaatcgac aaaattagtc ggaattcgca
     5761 tgggtgctgg gtttgcgggc ggtgatggcg cttgtcaagc ctagaattca ggcctgctct
     5821 gggcccgccg gcggcttggc gggcgccgaa ggacttctgt tttcatagct gctcgcgcat
     5881 atcccgcttg cgctgagggg tgttttatcc tgctttactg attcaaaccc acgatggaag
     5941 cgtttctgat atccacgggc atcgtggctt tggccgaaat gggcgacaag actcagttgc
     6001 tctcgctggt actggccgcg cgttttcgca agccgtggcc gattgtgttg ggaattttcg
     6061 tcgccacgct ggccaaccac gcgttggccg gtgcggtggg cagctgggtc acgcgcatgc
     6121 tggggcccga tgtgctgcgc tgggtgctgg gcgcttcgtt catcgccatg gcgatctgga
     6181 tgctgatccc cgacaagctc gatgaagacg accagcccag caccacgtcg aagctgggcg
     6241 tgttcggcac cacgctggtc gccttcttcc tggccgagat gggcgacaag acgcagatcg
     6301 ccaccgtcgc gctggcggcg caataccagc aatgggccgc cgtggtggcg ggcaccacgc
     6361 tgggcatgat gctggccaac gcgccggtgg tgtggtttgg cgaccggctg gtcaagcgcg
     6421 tgccgatccg cgtggtgcac gtgatctcgg cactgatctt tgccgtgctg ggcgtggtgg
     6481 cgctgctcgg cgtgggcacc tgagtggcgg ccgctatact gcaccttcag cgccgatttg
     6541 ctctgcttgc gggcgcttaa caaataccgc taaatccgag acgcgcgcgc aacccggttt
     6601 ctgatttggc tgaaaccggg ttttttgttg ccatgtcgtt catcgccacg ccgcaatccc
     6661 tgcatttcga tgcgccgctg ccgctgcaaa gcggcgcgtc gatccgcggc tacgacctca
     6721 cttacgaaac ctatggcgag ctgaacgccg cgcgcagcaa cgccgtgctg atctgccacg
     6781 cgctcaacgc ctcgcaccat gttgctggcg tgtacgaggc ccaggcgcgc agcgaaggct
     6841 ggtgggacaa catggttggg ccgggcaagc cggttgatac ccaccgcttt ttcgtcatcg
     6901 gcgtcaacaa cctgggctcg tgctttggct ccaccgggcc gatgcacgtc aaccccgaaa
     6961 cggggcaggt gtatggcgcc gactttcccg tcgtcaccgt cgaggactgg gtcaacgcgc
     7021 aggcgcgctt gctggacgcg ctgggcatcg aacaactcgc cgcagtcatg ggcggcagcc
     7081 tgggcggcat gcaggcgctc agctggacgc tgcaatatcc agagcgcgtg cggcatgctg
     7141 tggtggtggc cagcgcgccc aacctgacgg cagagaacat cgccttcaac gaagtcgcgc
     7201 gccgcgccat cgtgaccgac cccgactttc acggcggcca cttctacgcg cacggcgtgg
     7261 tgccggcgcg cggcctgcgc atcgcgcgca tgatcggcca catcacgtac ctgagcgacg
     7321 acgtgatgaa cgagaagttc ggccgcgagc tgagagaagc cgtggcgggc aacgacagcg
     7381 gctatcgctt ctcgacgcaa gacgtcgaat tccagatcga aagctacctg cgctaccagg
     7441 gcgacaagtt cagcgaatac ttcgacgcca acacctacct gctgatcacc cgcgcgctcg
     7501 actacttcga ccccgcgcgc gcctttggcg gcgacctgac gcaagcgctg gcccgtacgc
     7561 gcgccaagtt cctgctggtc agcttcacca ccgactggcg ctttgccccg gcgcgttcgc
     7621 gcgagatcgt caaggcgctg cttgaaaacc gccgcgaagt cagctacgcc gagatcgacg
     7681 cgccgcacgg tcatgatgcc tttttgctcg atgacgcccg ctacatgggc gtggtgcgct
     7741 cttatttcga gagtatcgcg gaggtgcagg catgagcgac gcacaggttc aacacaccat
     7801 cgcgcagctc gtgcccgagg gcgcgcacgt gctcgacctg ggttgcggcg acggcgcctt
     7861 gctgcagcac ctgatcacca cgcgccgctg caccggctac ggcatcgaga tcgacgacga
     7921 caacgtgctg gcctgcgtgc ggcgcggcgt caacgtgttg cagctcaacc tgcacgaagg
     7981 cctgaaggcg ttccgtgacc agagcttcga cgccgtgctg cagatcgaca cgctgcaaca
     8041 cctgcgcaat gccgaagtca tgctgcgcga aacggcgcgc gtgggccggg tcggcatcgt
     8101 ggccttcccc aacttcgcgc attggccgaa ccgcctgagc atcgcgcgcg gccgcatgcc
     8161 ggtgacgcgg cgcctgccct accagtggta cgacacgccc aacatccgcg tcggcacctt
     8221 caaggacttc gaggcgctgg cgcgcaagaa cggcctgcaa atcctcgacg ccttcgggct
     8281 ggaagacggc cgtgtggtgc gctggctgcc caacgcgcgc gccggcacgg cggtgttcaa
     8341 gttgcagcgg gcgtgagcgt gttttgaatg atttcggccg cttgcgcatg tctggcaagc
     8401 gcaagcagct atcaaatgca tagtccacag gattgaccat gtacccgacc atcgaccaac
     8461 ttgccgccga cctcgccgct ggcagcacca cgtcactcca gctgacggaa gccgcgctgg
     8521 cccgagcgca agacccggcg ggcgagggcg cgcgcgtgtt cacgctgatc gatgccgcgc
     8581 gcgcccgcgc cgccgcccag gccagcgacc tgctgcgcgc agccggcatg gtgcgctcgc
     8641 cgcttgaagg gctgccggtg tcggtcaagg acttgttcga cgtcgccggc caggtgacca
     8701 cggcaggctc ggtggtgctg aaagacgcgc cgcccgccga gcgcaacgcg cccgtgatcg
     8761 accgcctgat cgccgccggc gcggtgatcg tggggcgcac caacatgacc gagttcgcgt
     8821 tctcgggcct ggggctgaac ccgcattacg gcacgccgaa gaacccgtgg gaccgcgcag
     8881 gcggcggccg catccccggc ggctcgtcca gcggcgcggc ggtgtcggtg accgacggca
     8941 tggcggtggt cggcattggc tcggacacgg gcggctcggt gcgcattcca tcggcgctgt
     9001 gcggcctcac gggcttcaag ccgacgcagc ggcgcgtgcc gatcgatggc acggtgccgt
     9061 tgtcgacctc gctcgattcc atcggcccgc tggccgccag cgtgcgctgc tgcgccatcg
     9121 cggacgccgt tctggccggc gagccgcccg tggtgcccgc cgcgcgcgat gtgcgcgacg
     9181 cacggctggc cgtgcccgag acgctggtgc tggacagcat ggacagcacg gtggccgcag
     9241 ccttcgagcg cgcctgtgcg cggctgcagg cggcgggcgc gcgcctgtcg cgcattgccg
     9301 tgcccgagtt cgccgaactg ggccgcctgc acgcgcgcgg cacgctggcc ggcgccgagg
     9361 cctgggcctg gcatcgcacg ctgctgacca cgcgcggcga cgaatacgac ccgcgcgtcg
     9421 gcctgcgcat gcgcggcggc gaggcgatgg gcgcggccga ctacatcgac ctgctggccg
     9481 cgcggcgccg ctggatcgcg caggtggagg cccgcatcgc cggcttcgac gcactgctga
     9541 tgcccaccgt acccatcgtt gcgccgcgca tcgatgcgtt gacgaacagc gacgaagcct
     9601 actttgcggc caacggcctg atgctgcgca acccgacgct gatcaatttt ctggacggct
     9661 gcgcgctgtc gctgccctgc caagccgagg gcgagccgcc cgtggggctg acgctggcgg
     9721 gcgccggcgg gcgcgacgcg gaagtgctgg cgctggggct ggcggtcgaa gacgtgttgc
     9781 gcagcgcgcg gtgagcgcgg tcggatcgag aatcggaaaa aaaaggccgc cagcgcttgt
     9841 ctggcaagcg ccagcagcta ctttatttgt agtatagagc tggcaatgac ggcgttgcgc
     9901 gccttcaggc gcggcccgtt tcgcgcgcca cgtcggccag aaaatcgccc tgcgtcaatt
     9961 cgctttgcag cacgtcgccc ggcacccagc cggggtaggt ggcgtgaaag ttggccacca
    10021 ggcggtcgag cggcaaatcg tcgaacgcac cgcgctcgtt gacgtattcc atgtgccggt
    10081 tgcccgcctt gtcgaaggcg tggtagatcg aatcgtgtac gccgtcgaag tcgagcggca
    10141 gcagaccgaa cttctcgcac agctcgatgt tgaaggccgg cgtggccttg cgccaggcgc
    10201 cctccagcca cagctcggtg tagccgtgcc agatgaacag gtcggtcttc atggtctcgc
    10261 gcatgcgttc ggtgctcatg tggttgcgca cgtcggcaaa gcccagccgc gccggtatgc
    10321 cggccgcccg cgccaccgcc gtcagcagtg tggccttggg cacgcaccag ccgtagccgt
    10381 tggccagcac agtgctggcg cgcatgccgt tgggtgacag gtcgatgcgg tacgggtcgt
    10441 agcgaaaacc gtcgcgcacc gcgtgcacca gcgccacggc gcgtgcgcgc tcacccgcgc
    10501 cctgcgcgtg ctcgcgcgca aaggcttgca cgtcggggtg gccggcgtcg atcaggggcg
    10561 tggcttgcag gtgctgcggg gtgggagtgt cgctcatggg ggcatcttat cggcgcacgg
    10621 tttgtcatgg gcggtggcga caatcgcgcc atgcatgccc gccccgcgcc aaacaatttg
    10681 cccaccgccg tcccctttgg cgccgcgctg cgcttctggc tcaacctggg ttgcatcagc
    10741 tttggcggcc cggcggggca gatcgccatc atgcaccgcg agctggtgga gcagcggcgc
    10801 tggatctcgg agcggcgctt tctgcacgcg ctcaacttct gcatgctgct gccggggcct
    10861 gaggcgcagc aactggcgac ctacatcggc tggctgctgc acggcacgcg tggcggcatc
    10921 gtggcggggg cgctgttcgt gctgccatct ctggtgctgt tgatcgtgct gtcggccgcg
    10981 tatctggctt ggggccaatt gccgtgggtg gcggcgctgt tccatggcat caaggcggcg
    11041 gtggcggcca tcgtgctgca cgccatgtgg cggctgggcg gcaagacttt gacgacgccg
    11101 ctggcgtggg cgctggcggg cggcagcttt gtggcgctgg cgtggtggaa gtggccgttt
    11161 ccggcggtgg tgctgggcgc cggcgccgtc ggctggctgg ccgcgcgcgt cgcgcccggc
    11221 cagctggtac cgcctgtggc cgcgccctcg gcggccgcgg agagcgctgc cgtgatgggc
    11281 gacgacacgc ccgcaccgcc gcacgcgcac ttctcgcgcc acgggctggc gcgcgtgctg
    11341 ctggcgggcg cgctggcgtg gctgctgtcg atgggcggcc tcaccgcctg gcaggggctc
    11401 gacgcgacgt tgacgcaggt cggctggttc ttcagcaagg ccgcgctgat cacctttggc
    11461 ggcgcctacg cggtgctgcc ctacgtgaat caggcagcgg tgcagcagca ccagtggctg
    11521 acggcggcgc agatgatgga tggcctggcg ctgggcgaga ccacgcccgg cccgctgatc
    11581 atgatcgtcg cctttgtcgg ccagctggcg gcgaccgccg aggcgatgcg cgcttgcggc
    11641 gtcaacggat gcggtgcgtg ggcgctgggc tggggtgcgc tcggggcgct ggtggccacg
    11701 tggttcacct ttctgccctc gttcgtcttc atcctggccg gcgggccgct ggtggaatcg
    11761 acgcgcggca agctgggctt taccgcgtcg ctggcggcga tcacggcggc ggtggtcggc
    11821 gtgattggca gtcttgcttt gttttttgta gcgaatgtcg ctctaccctc gggcgctggg
    11881 gccggatggg actcaaaaat cgactgggcc gcactggcgc tggtggccgc ggcgaccctt
    11941 gcgctggtgc ggctgaagtg gggcgtgatc cgcgtcatcg cgctgtgtgc gctggcgggt
    12001 ggcgcgtggc gctggttcgg cgtgggctga aggcgctcct cagcgcatcg cccaacgcgc
    12061 gggcaattcg gccattgacg cgaacacctc gtgcgcgccc gccgcgcgca gcgcgttggc
    12121 ctgctcggct tgcgggcagt aggcccacac ggtggcgccg gcggcgacgc cggcctcgat
    12181 gccggtgggc gtgtcctcga tcaccaggca gcgcgcaggc gccacgccga gcgattgtgc
    12241 ggcggcgagg tacacatcgg gaaaaggctt gctgcgcggc agatcgtggc cgctgaagac
    12301 gtgcgctcca aaccattcgg ccaggccgac cttggccagt tgcagcagca ccttgccgcg
    12361 gtcggcgccc gatgcgacgg cgatgcgtcc gcctgtcatg tcgtgcgccg cgcgcacggc
    12421 ttccagcgcg ccggcgatgg gctgcaggcg ggcctccagt tcggcgttgc ggcgcgcgta
    12481 gaaggtggcc atccactcgt ccgtcagcgg ctggctggtg cgcgcttcga tcaacgccgc
    12541 ctggctgcgc accgtgcggc cgatgaagtg gcgcatgcat tcggccagcg gcatgtccca
    12601 gccactttcg gccagcagat cgcgcagcac gccatgggtg atcggctcgc tgtcgaccag
    12661 aacgccgtcg cagtcaaaca ggatgacttc aaatttcata gctgtttgcg cttgtctggc
    12721 aagcgctgga aggtgttctg atgaaaaaat gactttcgct tcgctcaatg gcgaaatgac
    12781 ccgcccgtct gtcatggcgc gtcagcgccg tcatcgcagg cgaagccaag gtcatttcgt
    12841 catgtgctgc tggccgcgcg catcaacgcc gccagaactg cggcgtgaac aagaccatca
    12901 ccgacagcag ttccagccgc cccagcagca tggccgccga gcacagccag atctggaagt
    12961 cgttcagcac cccgtagttg cccgccggcc cgactttgcc caggcccggg ccgatgttgt
    13021 tcaggcaggc cacgatggcg gtgaaggcgg tcaccacgtc caggcccgag gccagcatga
    13081 gcagcgtcat gccgatcatg gtgccgccgt agatcagcat gtaggccatc acggcccaga
    13141 tgacgtcggg cggcacggcg gtgcggccaa gcgataccgg gtacaccacg cgcgggtgga
    13201 tgatgcgcac cagctcgcgc tgcgcgatct tcaccagcac cagcacgcgc accattttga
    13261 tgccgccgcc cgtggagccg gcgcacgaca cgaagcagcc caaaaacaag agcagcaccg
    13321 gcacgaagga tggccacagc aggtagttgg tggtggcgta gccggtggtg gtggccaccg
    13381 acacgacctg gaacgctgcc tgccgaaaag cgctgggaaa gtcgtcgatc acgtgctcca
    13441 tcaacagcat ggcgcacacc agcagcgtgc acagcagcaa ggtgccgatg taggcccgca
    13501 cctcggtatc gcccagcacg ggcgacagcg agcgctggcg ccacagcatg aagtagcgcg
    13561 caaagctgat gcccgacagg cccatgaaca gcaccgccgc ccattccaga tgcggcgcgt
    13621 tccagtagcc aaagctctgg tcgtgcgacg acatgccgcc gagacccacc gtggtgcaca
    13681 tgtgcatgaa ggcgtccgac cacgtcatgc cgcccgcgcg ataggccaga aagcagccca
    13741 gcgacagcac gaagtacacg ccccacaagc cgcgcgcggt ttcggccagg cgcggcgtca
    13801 ggcgctgctc tttcatgggg ccgggcatct cggccttgta gagctgtgcg ccgccaagac
    13861 ccagaaacgg aaggatggcg accgccagca gcatgatgcc gaggccgccg atcagctgca
    13921 gaaagcagcg ccagatgttg accgaaggcg ccagcaaatc cagccctgcc agcgccgtgg
    13981 cacccgtggc cgtcagcccg ctgacggctt cgaaatacgc atcggtgaag ctgatgccct
    14041 gcaccgtgaa caacagcggc agcgaggcat aggccggcat caccacccac accagcgtga
    14101 ccagcaagaa gccgtcacgc accgtcagtt cgcggtgtgc gtgccgcgtg cgcacccaaa
    14161 gccagccgcc ggtggctagc gtgaacagaa agcaccagcc ccacacccgc acgtggtcgc
    14221 cgccttcgcc aaaccagtcc cacgccatgg gcacgaaaaa ggcaaacgag aagcccatga
    14281 cgacccatga cagcacgagg aaggcagcgt cgaagcgctt catggcgcca cccccaacgg
    14341 ccgcgaagca ggcgatgccg gaccgacgtg gccgggcttg caccggacac cagcgttgtc
    14401 cccccggggg gaaggcgccg caggcgactc agggcgggtc ataagaatgt tgcacggacc
    14461 tggaacaact tctccacctc gcgcaccatg cgcttgtgcg gcaggaagat gatcacgtgg
    14521 tcgtctgtct cgatcaccgt gtcgtgcgtc ggcaccacgg tgcgcgcgcc ttcgccttcg
    14581 ccgcgcacga tggcgccgat gcgcgcaccc gccggcagct tgatctgctc gatgggccgg
    14641 ccgaccaggt ggctggtctt ggcgtcgcca tgcgccacgc cctccagcgc ctcggccgtg
    14701 ccgccgcgca ggctgtgcac ggccgccacg gcaccgcgcc gcacgtgcat cagcagctcg
    14761 ccaatcaccg cgtgcgcggg cgaaatggcg atgtcgatgg tgctgccctg cagcatgtcg
    14821 gcataggcgc ggcggttgat gagcgcgatg acgcgccgcg cgccgagccg cttggccagc
    14881 atggccgcca ggatgttgtc ctcgtcgtcg ctggtgacgg cgatgaacaa gtccatgtcg
    14941 gccacgttct cgtcaaccag cagttcctcg tcggtgccgt cgccctgcag caccagcatc
    15001 tcggacggca gttcggacgc caggtaatcg cagcgcttgc ggttggcctc gatgatcttc
    15061 accgagcact gaccgaccag gccgcgcgcc agccgtaggc cgaccttgcc gccgccggcg
    15121 atcatcatgc ggcgcaccgg gcggtccggg ttgccgatgg cgtgcagcgc gcggcgcacg
    15181 ctttccttgc ccgtcagcac gaacacctcg tcgccggcac gcacgcgcgt ctcggcctgc
    15241 accggcagtt cttcatcgcc ccggtaaatg gcgacgatgc gcatcggcac gtcggccttg
    15301 tgggcgcgga actcggcaat gctgtggtcg gccaggtagc tgccggccga cacgcgcacc
    15361 accaccaggt gcgcctggct ttgcgagaac ttcaccacct gcagcgcttc ggggtagtcg
    15421 atcagctggt gaatggtgcg cgtgaccgat tcctcggggc agatcacgta gtcgaccgag
    15481 aagccgtcct tgtccagcag cttgtcgccc tggctgaact cgggcgagcg caggcgcgcg
    15541 atggtggtcg ggatgccgaa cacgtcgtgc gcgatcttgc aggccaccag gttggtttca
    15601 tcgagcggcg tgcaggcgat gaacaggtcg caatcgtctg cgcccgcggc gcgcaatatc
    15661 gatggctgca cgccgttgcc ggcgacgccg cgcaggtcga gtcggtcttc cagcgcgcgc
    15721 aggcgctcgg ggtcggggtc gaccacggtg atgtcgttgg cctcggacac cagcgtctcg
    15781 gccacgctct cgcccacgcg gccggcgccg aggatgatga tcctcatgcg gcgctcccgg
    15841 taccacgcag cggtcgttgt ggtttcttgc gcatcaccat gatcactctt gttttaatag
    15901 ctgctcgcgc ttgctgggaa agcgctggcg gccaaaatcg ttcaaaggag ggtgccatcg
    15961 acataaaacc agcgcgccac gccattggcg ccattctcgc gcacgaagca gctctgttca
    16021 tgcaggcgcg tggcgcggcc gtgcaggcgc gagcgggcga cgaattcgac ctcggcatgc
    16081 gcgtcgtcca gccgccggcg cgcgcgcacc tccaggccca gccactgagt tccagtgtcg
    16141 aagtcgagcg tggcgggtcg cttggaaggg tgccaggtcg cgagcaggta ttcggcccgc
    16201 tcacacacga aggcgctgta gcgcgagcgc atcaggcttt cggcatcggg cgctggcgtg
    16261 tcgctgaagt ggtcgatgta gcggccgcag caggcggccc agtccagcgg ccggcgcctg
    16321 gcatccagcc ggccacaggg gcaggcagcg gcggcgggtg tcatggcgcg gcttactttg
    16381 cgcgccgcgc gcggtgcgcc tcgtagctct cgaccggcgc gccttgcttc gcccattcgg
    16441 tgaagccgcc atcgatgtgc gccacgttgg tcatgcccat gtcctgcagt gccttggcgg
    16501 ccagcgcgct gcgccagcca gcgccgcaaa acagcacgta ctgcttgtcc tcgtcgccga
    16561 acaccggctt gtaatagggc gaatcgggat cgacccagaa ttccagcatg ccgcgcggcg
    16621 cgttgatgac gccgggcacc gtgccctcgc gctccagctc gcgcgcatcg cggatgtcga
    16681 ccaactgcag cgccgggtcg tccagccgct ggcgcacttg atcgacgctg tgcgtcgtga
    16741 cctgcgccat cgcctcgtcg accagcgcgc gaaaaccttt ggtgatgggc atggtggggc
    16801 tccttacagc gcgcgggtga tgatgatctt ctgcacgtcg ctcgtgcctt cgtagatctg
    16861 gcacacgcgc acgtcgcggt agatgcgctc gacgggaaag tcgctcacat aaccatagcc
    16921 gcccagcgtc tggatggcgg cgctgcacac ggcctcggcc gtttcggtgg cgaacagctt
    16981 ggccatggcg gcttctttca ggcacggcag gccggcatcg cgcaggctgg cggcgtgcca
    17041 gatcagctgg cgtgctgctt ccaacctcgt agcgcagtcg gcaaggcgga agcccaccgc
    17101 ctggtggttg aagatggcgg tgccaaagct ctcgcgctcc ttggcgtact gaatggccac
    17161 ttcaagcgcg ctgcgcgcca tgcccacgct ttgcgccgcg atgccgatgc ggccgccttc
    17221 cagcgcactc agcgcgatct tgtagccccc gccctcttca ccgatcaggt tctcggccgg
    17281 cacacggcag ttttccaggt tgatctgcgc cgtgtcgctg ctgtgctggc ccagcttgtc
    17341 ctcgatgcgc gccaccaccc agccgtggtt gcccaggcgg tgcgtgggca cgatgaaggc
    17401 gctcatgccc ttcttgccgg ccgccttgtc ggtgacggca atcaccaccg ccgcgtgccc
    17461 gttcttgccg ctggtgatga actgcttgac gccgttcagc acgtactcat cgccgtcttt
    17521 caccgccgtg gtgcgcaggc tcgacgcatc gctgcccacg tgcggctcgg tgagcgcgaa
    17581 ggcgcccagc atctcgccgc gcgcggcggg cgccagccac ttctgctttt gcgcctcggt
    17641 gccgtacttc atcaagatgg cgttgtaggg gcagttggtc acgctgatgg gcgtactggt
    17701 gccgccgtcg ccggcggcaa cttcctccag caccagcgcc agcgtgatgt agtcgaggcc
    17761 cgcgccgccg tattcctcgg gcacgcagat gccgtaggcg ccgagttcgg ccaggccctt
    17821 gtgcgcggct tcggggaagg cgtgttcctt gtcccactgc gcggcgtgcg gccacagttg
    17881 ctcttgcgca aagctgcgca cggcgtcgcg aatcatttcc tggtcttggg tcagcagcat
    17941 gtggtcacca tgtgtcgaag cgttgtccgt cgtattgaat gtaggcgccg cggtcggccg
    18001 gcgtcagcgc ggccattacg cggcgcagcc cgctggcgct ggcctcgggc gtcaccggcg
    18061 cggcgccgcc gcccatgtcg gtctgcaccc agcccgggtg caggcacacc atgatcgcat
    18121 tcggccaggc gctttgcgcc gacgctaccg ccatgttcag cgccgccttg ctggtgcggt
    18181 aaacccaggc gcggctgttg tccaccgtgg cgatgcggcc cattacgctg gagacaaagc
    18241 caaagcgacc acccggggcc agcagcggcg cgacctgcgg aatcgcctgc atcgcgccca
    18301 gcacgttggc gtgcatcacg gtgtcgaagg cttcgcgcgt gggcggctcg gtggcatcgc
    18361 cgtggctggt gacgccggcg acgtagagcg cggtgtcgat cttctcgcca tcgagttgcc
    18421 aggccaggcc gctgatgctg gcggcgtcgg ccacgtccac gcgcagcggc tcggcgccga
    18481 tgctgcgcag gcgcgccagg gccgcatcat cgcgcgcggt ggcgatcacg cggtcgcccg
    18541 cttcggcgta ttgccgcgcc agctcccagc caatgccgcg cgaggcgccg atgatcagaa
    18601 cggtggacat gaatgcgtca tcccttcacc agatgcgcca ggccttcgtc ggccagttgc
    18661 gcgcggtagc gcgccagcgc ttcgtcgtcg gtctcggtca ccacctgcgg gccgagttgc
    18721 gccagcagca tggcgttcat cgacgggaag ctgcccgccg ccacgcgcgc ctcgggcgac
    18781 cacacctgcg agcgcagcag cgccttgggg cagtgcagat acgcctcgcg caccgccact
    18841 tcgatcacca ggcgcggcag cttgggcgcg tcggcgtgcg caaagcgctg cgtgaattcg
    18901 ggctcgtcgc gcagttgcgc cgtgccgttg acgcgcagca cctcgttcac gccggggatc
    18961 aaaaacagca ggccgatgcg cggatcggcc agcaggttct ccagcgtgtc gagccggttg
    19021 ttgccagtgt ggtcggggat cagcaacgtg cgctcgtccg ccacctgcac aaagccgggc
    19081 cggccgccgc gcggtgatga atcgagcccc tcacccgccg cgcccgcgct ggcgatgacg
    19141 cacagcggcg cgtgcgcgac gaagcggcgc atggtgtcgt ccagccgatc cagctgcttg
    19201 gcgcgcgaac gcggcgacgc ttgcgggtac aggcggcgca gggtgtcagc ggagtcgatc
    19261 atctcggcat gctttgagga atatgaatca aacaggccgc cagcgcttgt ccatcaagcg
    19321 ctgacagcta tgaaattcat accaactcca gcgccagcgc ggtcgcctcg ccaccgccga
    19381 tgcacagcgt ggccaggccc ttttttttgc cgcgcgcctt caaagcatga atcagcgtga
    19441 ccatgatgcg cgcgccgctg gcgccaatcg ggtggcctaa ggcgcaggcg ccgccgttga
    19501 cgttgacctt gtcgtgcggc accttcagat cgtgcatcag cgccatgggc accacagcaa
    19561 aggcttcgtt gatttcccac aggtcgacgt catcgacctt ccagccggcc ttttccagcg
    19621 ccttctcggt cgcaccgatg ggcgccgtgc tgaaccattc cggctcctgc gcatgcgtgg
    19681 cgtggctgac gatgcgcgcc agcggcttga cgcccagctt cttggccgtg ctctcgcgca
    19741 tcatcaccat ggcggcggcg ccgtcgttga tgctggagct ggcggcggcc gtcaccgtgc
    19801 cgtctttctt gaaggcgggc ttgagcgtgg ggatcttgtc gatgttgatc ttgcccgggc
    19861 cttcgtcggt gtcgaccacg cgctcgccct tgcggtcttt caccgtcacg ggggcgattt
    19921 cgtccttgaa tgcaccgttt ttgatagcgt cctgcgcacg ccgcacgctg gccatggcga
    19981 acttgtcttg tgcgtcgcgg gtgaagttgt acttggccgc gcagtcctcg ccaaaggtgc
    20041 ccatgctgcg gccggcttcg taggcgtctt ccaggccgtc gagcatcatg tggtcgtaca
    20101 ccttgtcgtg gcccatgcgg tagccgccgc ggcctttttg cagcaggtag ggcgcgttgg
    20161 tcatgctctc catgccgccg gccaccagca catcgtgcgt gcccgcgacc agcatgtcgt
    20221 gcgcaaacat ggccgcgcgc atgcccgagc cgcacatctt gctgagcgtg accgcgccgg
    20281 cgctcttggg cagcccgccc ttgaatgcgg cctggcgcgc aggcgcctgg ccttggcccg
    20341 ccatcaggca gttgccgaag atgacttcgg tgaccagttc gggctggatg ccagcgcgct
    20401 cgacggcggc cttgatggcc gcgccgccca ggtcgtgcgc ggcgagcggc gtgaagtcgc
    20461 cctgaaaact gcccatgggg gtgcgggcgg cggaaacgat gacgatgggg tcggacatga
    20521 aagaagctcc ttgaaataaa cgtgaaaatc ttcgattcag tcagtgcact tgcggtcgat
    20581 gcaggacagc gaagcgatcc gccaggccgg ccagacgatg atcgagtgtc cataaccgcg
    20641 cgccgggcgt catcagggtc gaggccagca acatgatgtc caccagcccg caacccagcc
    20701 cgaacaagtg ttcgttctcg atgaattgaa gggcttcgtc cacgctggcc tgctgggccg
    20761 gttgcaacaa cgccaggtcg gccagggttt ggtgccgctc gggcggtgtg ccgcaggcga
    20821 cttctccgac aacgagtgga tgtccgagca cttggtcaag ccccagcaaa ccgatgagcg
    20881 cgtcgttgcg acggcgaaag tgatcgaccc acacggaggt gtccaccagc acgccactca
    20941 tgcgctggcg gcgcctttgc ggcgcggcac atcggacatt cgcggcgctg cagcacccag
    21001 cgcggcgaga cgtctggccg cttgcacccg cacgaaggtt ttgatggcct cgcggaacag
    21061 gtcggcctta tccatatcgg ggtcggcgac ttccagcgct ttttcgtaca gcgcgtcgtc
    21121 gatggtgacg gtggttctca taagagcgag cctcaatatt gatgtcaaat agcatcttaa
    21181 ttgatgcaat tcaatggggc aagttgcgtg caatgccggc cacggcgaag cctttggcgc
    21241 ggcgacttgg tcactccgac gactccgccg acgtgaccgg cgccgacgta tcgggttgat
    21301 cggccggcgt ctccacagcg aaagcggccg ggtcaggcaa ctccgacagg cccgtgacgc
    21361 cgccattcac ctcaaaccgc cggtgcaccc catacggaaa gaagtcaatc acctccccct
    21421 gctgaattcg cgccttggtc tgctgccaga actcgggctc cagcaggtcg gcgtggtggc
    21481 gcatgaaggc ctcgcgcacg cgcgggttgc cgagcaggaa ggggccgaag gtttcgggga
    21541 agacgtcgtg cttggcgacg ctgtaccaaa tctcgccgct catttcgtct tcttcgttgc
    21601 gcggcggcgg cacgcggcgg aagttggtgt cggtgatgta ttcgatctcg tcatagtcgt
    21661 aaaacaccac cttgccatgc cgcgtgacgc caaagttctt ccacagcatg tcgccgggga
    21721 agatgttggc ggccaccagg tccttgatgg cgttgccgta ttcgatgacg gcgtgttcca
    21781 tctgagcatg tgccgcaggg tcgtcacccg cgtcgaacgc ttcctgcagg tacaggttga
    21841 gcggaatcat gcgccgctcg atgtaggcgt ggctgatgat gacctcgatc tggccgtcgc
    21901 cgtcgcggtc tgaaatttcg agctggctgg gcgcgaactt ctcgatctcg gcgatcaggt
    21961 cgtcatcgaa tcggtcacgc ggaaaagcca gcaaagagaa ctccatggtg tcggccatgc
    22021 ggcccacgcg gtcgtgctgc ttgacgagct ggtacttgcc gcgaatctgc tcgcgcgtgg
    22081 tctccttggg cggcggatac cagtccttga tgaccttgaa cacgtaggga aagctgggca
    22141 a
//
LOCUS       DAOMVO010000010        21534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_415_length_21534_cov_14.334978, whole genome shotgun sequence.
ACCESSION   DAOMVO010000010 DAOMVO010000000
VERSION     DAOMVO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 21534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21534
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_415_length_21534_cov_14.334978"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="coxB"
                     /locus_tag="PKV30_01190"
     CDS             complement(<1..910)
                     /gene="coxB"
                     /locus_tag="PKV30_01190"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRL28866.1"
                     /translation="MKSIASKLAGVPAQAAIWGAAWWAGAASAQVNDLPGGPAVRQLN
                     LPPPVTKIAEAQSGLHWMMLIICTIIFIAVFSVMFYSIWKHRKSQGAKPAAFHESVAV
                     EVAWTVVPFLIVIGMALPATKVVVAQKDTSNADLTIKATGYQWKWGYDYLNGEGAGIG
                     FLSTLDSSQRALSSAGKPAGDDYLLKVDNPLVVPVGKKVRIITTANDVIHAWMVPAFG
                     VKQDAIPGFVRDTWFRAEQTGDFYGQCAELCGKEHAYMPIHVKVLPQQEYSAWVADQQ
                     KRMAALADDPNKVWTLPELVARGEKVY"
     gene            complement(956..1486)
                     /locus_tag="PKV30_01195"
     CDS             complement(956..1486)
                     /locus_tag="PKV30_01195"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRL28867.1"
                     /translation="MLFRLGAETVALQQTSAYRWARVSDQALAWHLRRNCSVTPRQLA
                     LLYAGLSAVSLTIGVGFWMMGATLVLGFAGLELVAVGAAFLIYARHAADGETVSLQGE
                     RLIVERECAGRRERLEFCCAHVRIAPPASGQGLIEVMAHGRRVDLGRHVRPEWRPGLA
                     GEMRSALQAMGRQAVC"
     gene            complement(1542..2465)
                     /locus_tag="PKV30_01200"
     CDS             complement(1542..2465)
                     /locus_tag="PKV30_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HRL28868.1"
                     /translation="MPPATSQSPPTIDPVAARRWALQPRHVSAWLHEEVARRVAERLD
                     VIRLPVRAWADWEPVRGGLAGHQLVAARYPDAACFQVLAHAPLAPEATKKRANDWWRK
                     WSRQQPEVIDVAQPPAASVQLVWANMLAHQSADPVALMGDWHRALAVDGFVMFSCLGP
                     DTLQELRALYARLGWPPPAHEFTDMHDWGDQLVAAGFAEPVMDMEHITLTFDSPARLL
                     QELRELGRNLHLQRFGALRGRGWHARLQAALAETQAAPASGGALSLTFEVIYGHAVRA
                     PPRHAVSSETAIQLGDMRAALRRRGQNPHGV"
     gene            2516..3241
                     /locus_tag="PKV30_01205"
     CDS             2516..3241
                     /locus_tag="PKV30_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRL28869.1"
                     /translation="MFTRLLHRIAAPLRADAAAWPGQCLICHAWPTTTLCGDCVARFA
                     PAQARCTTCALPVPPGVTVCGQCLRKPPPMAQCLAAVNYRWPWSMCVARWKFEGDVGL
                     TRPLATLLQSTPGVRAALDAADLVLPMPMSEQRLAERGHNPALLLARRLAPLTTRAGL
                     LLRTRDTPPQRSLPRAARLKNVRGAFQVDPLAAPSLAGRRVVLVDDVMTTGASVREAA
                     SVLSAAGAAHIAVLVLARTDEPA"
     gene            3265..3732
                     /gene="trmL"
                     /locus_tag="PKV30_01210"
     CDS             3265..3732
                     /gene="trmL"
                     /locus_tag="PKV30_01210"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRL28870.1"
                     /translation="MFRIVLVEPEIPPNTGNVIRLAANTGCALHLIEPLGFSMDDKQL
                     RRAGLDYHEYASVRRHADWHAFMQAEQPDPARLFALSTRGTRHVHDISFVAGDWLVFG
                     AETRGLPSHVRTAIPTAQQLRLPMRAGQRSLNLSNAVAVTVFEAWRQNGFVGA"
     gene            3897..4223
                     /locus_tag="PKV30_01215"
     CDS             3897..4223
                     /locus_tag="PKV30_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28871.1"
                     /translation="MIQSLKPLAQAALNVGRFVTGPRTATTADMARIRRAMGEQVLDC
                     ELRVGRRVRTQLDAASTVMQLWLLRSEIYQAVSDQFGQQEAMRRVKLLTPEFEGLLPE
                     RQLRSV"
     gene            complement(4255..4707)
                     /locus_tag="PKV30_01220"
     CDS             complement(4255..4707)
                     /locus_tag="PKV30_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRL28872.1"
                     /translation="MKTFNTLAELAALAGQEITVTDWYEVTQQHINQFADATGDHQWI
                     HVDAERAKAGPFGTTIAHGYLTLSLLAGFFDRALSVKDVRMGINYGLNKVRFMGPVPS
                     GSRLRVRIALLGSEPIERGLQMAWKCTIEREGQEKPVCVAEPLSRLYL"
     gene            complement(4771..5391)
                     /locus_tag="PKV30_01225"
     CDS             complement(4771..5391)
                     /locus_tag="PKV30_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRL28873.1"
                     /translation="MPVVVIANPKGGVGKSTLATNVAGYWASQGHAVMLGDVDRQQSS
                     RLWLGLRPPAAPPITSWEVQRDAVVRPPKGTTHVVLDTPAGLHGGKLKEVLRIADKVL
                     VPVQPSIFDIYATREFIDQLLEGKGADRRDIGLIGMRVDERTIAADQLRQFTDGLGLP
                     VLGTLRPTQNYVYLAARGLTLFDVGPGRVARDLEQWQGITAWLDKA"
     gene            complement(5536..6285)
                     /locus_tag="PKV30_01230"
     CDS             complement(5536..6285)
                     /locus_tag="PKV30_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL28874.1"
                     /translation="MNLITDWQFYVVAVPAVLLLGVSKSGFGAGFGSLAVPLMALSVT
                     VPQAAAILMPVLLVMDLLGIAAFRKDFDRALLKFMLPWGLLGIVIGTLSFKLLEARWV
                     AGIVGVFTLLFLAQRLLFAPKAGDAPPPRWAGALLTATSGFTSFVAHAGGPPINAYVI
                     PLGLSPVRFTGTMAYFFFVINLAKWVPYAWLGLLDARNLGTSLVLLPLAPLGVWIGVR
                     IARRINQQLFYRFIYVGMLLTGLKLIWDAVV"
     gene            6422..7072
                     /locus_tag="PKV30_01235"
     CDS             6422..7072
                     /locus_tag="PKV30_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRL28875.1"
                     /translation="MELLQPLSPLWATRLHIALELAATLAFALSGVIEAARKRLDVVG
                     VAVVAFAAAFGGGTLRDLLLDYRPFFWVQQIEFLWIVLALCAGAMLFMRQRHLRPTER
                     AILWPDAIGLGLFTAIGVDRALALGQPALVAVMMGVVTSVFGGVLRDVLVNEVPRALS
                     DHRPYALWAFAGGWLYVVLLGAALPPWLALSITVVATAGLRVLSVYRDWQLPSWRA"
     gene            complement(7262..8254)
                     /locus_tag="PKV30_01240"
     CDS             complement(7262..8254)
                     /locus_tag="PKV30_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL28876.1"
                     /translation="MTRNTLPRRRALTAASALALAAALPGVALAQAYPNKPITMIVPF
                     PAGGTTDILARIMGKALEGELGQPVVIDNKGGAGGNIGAQAAARAAPDGYTLMMGTVG
                     THGINEALYSKLPFSPLGDFQPISRVAMVPNLLVTHPSRPYKTVKELIEFAKANPGKV
                     NFASSGSGTSIHLSGELFKAMTKVDMTHVPYKGSAPAVADLLGGQTDIMFDNMPSVMQ
                     HVKGGKLRAIAVTTDKRYPGLPDVPTIAESGVPGYNATSWFGLFTNRKVPPDVLAKLS
                     AATMKVLAKPEVVQQMAEQGAIAHPEKPAEFEAFVKAETAKWGPVVKQSGARVD"
     gene            complement(8358..9062)
                     /locus_tag="PKV30_01245"
     CDS             complement(8358..9062)
                     /locus_tag="PKV30_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL28877.1"
                     /translation="MTQRERVLITGGASGIGAATAERCRAEGWDPVVIDSVGDGIRAD
                     LSSADDTARALQEALAGGPITRLVNNVGIVVPNDAEHQTLQEFDLAVSLNLRCAFQCM
                     QALLPGMRAAGFGRIVNMSSRAALGKELRTAYSATKAGLIGMTRVWALELGAHGITAN
                     AIGPGPIRTALFDKANPPDAPRTQAIIDAVPVKRVGTPYDVAHAVAFLLDAKSGFITG
                     QVLYVCGGMTVGVAGV"
     gene            complement(9062..10264)
                     /gene="pcaF"
                     /locus_tag="PKV30_01250"
     CDS             complement(9062..10264)
                     /gene="pcaF"
                     /locus_tag="PKV30_01250"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRL28878.1"
                     /translation="MKQAFVVDAIRTPFGRYGGALSSVRTDDLGAVPLKALMARNPKV
                     DWAAVTDVIYGCANQAGEDNRNVAHMASLLAGLPVDVPGATINRLCGSGLDAIGTAAR
                     AIKAEEATLMIAGGVESMSRAPFVMPKAESAFSRTNAVYDTTIGWRFINKLMKAQYGV
                     DSMPETAENVATDYKIEREAQDKMAMASQMKAVAAQKAGHLAREITPVSIAQKKGDAV
                     LVDTDEHPRETSLEKLASLKGVVRPDGTVTAGNASGVNDGACALLLADEATAGRYGLT
                     PKARVVGMAVAGVAPRVMGIGPAPATEKVLALTGLKLDQIDVIELNEAFAAQGLAVLR
                     MLGLKDDDARVNAWGGAIALGHPLGASGARLATTAVNRLHATGGKYALCTMCIGVGQG
                     IALVLEKA"
     gene            complement(10322..10969)
                     /locus_tag="PKV30_01255"
     CDS             complement(10322..10969)
                     /locus_tag="PKV30_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRL28879.1"
                     /translation="MSSYQKRSKDELARRVAQDIPDGAYVNLGIGQPTAVANHIPPGR
                     EVILHSENGILGMGPAPAEGTEDYDLINAGKQPVTLLPGGALFHHADSFAMMRGGHLD
                     VCVLGAFQVSTKGDLANWSTGEPGAIPAVGGAMDLAVGARQTWVMMDLLTKKGESKIV
                     AQCTYPLTAIGCVKRIYTDLATFECTPQGLRWVDSVDGLALDELQRLVGQPILVE"
     gene            complement(10998..11714)
                     /locus_tag="PKV30_01260"
     CDS             complement(10998..11714)
                     /locus_tag="PKV30_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRL28880.1"
                     /translation="MIDKIAPTVAAALADVPDGATVLIGGFGTAGVPAELIDGLIAQG
                     ARDLTLVCNNAGNGDHGIAALIKAGRVRKIICSFPRQADSHVFDAAYRSGKIELELVP
                     QGNLAERMRAAGAGIGAFFCPTGFGTELAKNADGAPKETRRINGRDYVLEYPIYGDVA
                     LVKAERGDRWGNLTYRMAARNFGPVVAMAAKFTVASVHDVVKLGELDPEAIVTPGIFV
                     SRVVQVPRVATQAGGFKQAA"
     gene            complement(11778..12695)
                     /locus_tag="PKV30_01265"
     CDS             complement(11778..12695)
                     /locus_tag="PKV30_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28881.1"
                     /translation="MVLGAVRAIPVLVRYRSPRDTREKKMKMYRLWLSAAGVALISAA
                     GSAHALVFSAVTQSPPPGYQLTAAGQAAGTPPVTVTYTPTPTQGTLWNPNPFAADTFF
                     NFNVDPGGNLVPTNTADNTFGATFFGQPGGTATYTFSQPVVGDAATGLTQLTTLNTMQ
                     HASFQFTLQGGAVWTAANLLPPPGSCTPPAQMHGLTTVSGLGTPVLNFTRPVGSSVEG
                     CDDYALVASGPFTTFQIAAVAPVNPRFGFIVNETLRARFTTLVQAPAAASGGLASVPA
                     VHGAGLVALAGLMALAGRRRLRQRIRRGA"
     gene            12707..13147
                     /locus_tag="PKV30_01270"
     CDS             12707..13147
                     /locus_tag="PKV30_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HRL28882.1"
                     /translation="MFAPTQQDVRRFFCSAWAKQQSGQPMETIETLAAGWIAEHPEWH
                     ADFSDAEAAVARDYGDGAVGGNPFLHLSMHLSISEQCSIDQPRGIRQAVELLAARRGD
                     LHAAHHEVMECLGQMLADAHASGRPPDGDGYIACVQRRATRDAL"
     gene            complement(13285..13950)
                     /locus_tag="PKV30_01275"
     CDS             complement(13285..13950)
                     /locus_tag="PKV30_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL28883.1"
                     /translation="MKTTLSPMFAAIVVALGAAMPAQAQVTGDAKAGEGKIAMCIGCH
                     GIIGYKSSFPEVYHVPKISGQNAKYIAAALEAYKKGDRKHPSMRGIATTMSEQDIADV
                     AAYYAQHGVKEGAPELPAAREPSAQVAQLLQKGACVSCHGDNFSKPIDPTYPKIGGQY
                     ADYLYVALKSYRDNTHHTWGRGNPVMAGVAKQFSNAELKAMAKYIASLQGDMTTVPES
                     RFR"
     gene            14068..14910
                     /locus_tag="PKV30_01280"
     CDS             14068..14910
                     /locus_tag="PKV30_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL28884.1"
                     /translation="MKFQGSENYVATQDLMLAVNAAITLKRPLLVKGEPGTGKTMLAE
                     EVAEALKLPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDDKVKDIHNYIVRGVLWQ
                     AFTADEPVALLIDEIDKADIEFPNDLLRELDRMEFHCYETRETVRAKHRPVVFITSNN
                     EKELPDAFLRRCFFHYIKFPEPETMKQIVDVHFPNLKKELLAAAMKVFYDVRGLPGLK
                     KKPSTSELLDWLKLLVAEDIPLEALQSADSKVSVPPLVGALLKNEQDVTLFEKLVFMN
                     QRNR"
     gene            14918..15163
                     /locus_tag="PKV30_01285"
     CDS             14918..15163
                     /locus_tag="PKV30_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28885.1"
                     /translation="MNPLLLEGLADALGFVGGALVGFLLARLAGFDLFAEGYGASSMA
                     AIVAVGVGAGLGRAVTRRWRLRREAARRAASAPSSKS"
     gene            15165..15758
                     /locus_tag="PKV30_01290"
     CDS             15165..15758
                     /locus_tag="PKV30_01290"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRL28886.1"
                     /translation="MSFVESVVLRDHGVRLEPLALSHEDGLRAAAADGELWKLRVTSV
                     PAPQETRAYIDTALKMREEGHRFAFAVLDDATGTVLGSTSYHDIVPALRRVEIGYTWY
                     RQSVQRSHVNTTCKLLMMGHAFDTLQCPVVGWRTDNYNFASQKAIERLGARKDGVIRH
                     HALRRDGTVRDTVMYSMTDGEWPEAKAQLLYLRARRG"
     gene            complement(15771..16472)
                     /locus_tag="PKV30_01295"
     CDS             complement(15771..16472)
                     /locus_tag="PKV30_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28887.1"
                     /translation="MTPIRSAHPSPIGPDAEERSLVYRKTEAGRQALAALRGHMALRD
                     RQIVLLCTGERNVVAMTEVFGPDTPAHLRRLVAAGLIEAAEPTAWPHVPPEFAPTLPP
                     LGGVAATSANQSGFGITRVPDEDSLQAARAQAGVMLIEIGGEAAEQLVKDSADTQAED
                     DTLALLARAIGLAVRAWGDTEALPWAEQIARLLPRASIARLLDCLIDEDSAALVAPLY
                     ERLLSDSEFGAERGQ"
     gene            16667..17854
                     /locus_tag="PKV30_01300"
     CDS             16667..17854
                     /locus_tag="PKV30_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRL28888.1"
                     /translation="MLIDFFYTLRAAKLPVSVKEFLTLLEALQADVVGPRQPDACSID
                     DFYYLARTALVKDEKHFDKFDRAFAAYFKGVEHIADFRKEIPAEWLRKILEKELTPEQ
                     KAAIEKMGWDELMETLKKRLDEQKERHEGGSKWIGTGGTSPFGHGGYNPQGIRIGGKG
                     GNKSAVKVWDQRAYRDYDDSQELGTRNIKVALRRLRKFARTGSEFELDLPDTIRSTAA
                     NAGWLDIKMVPERHNSVKVLLLMDVGGTMDEHIARVEELFSATKAEMKHLEFYYFHNC
                     VYDFMWKNNRRRFAEKFATWDIIRKYNKDYKLIFVGDATMSPYEILQPGGSVEYNNEE
                     PGAEWLQRLTHAFPKFAWINPEPQGVWQYRQSIAIIQQLMSHRMFPLTLKGLEDAMRM
                     LSK"
     gene            complement(17913..18461)
                     /locus_tag="PKV30_01305"
     CDS             complement(17913..18461)
                     /locus_tag="PKV30_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRL28889.1"
                     /translation="MAQAGVNLPGFNTPAVGFEQPFEMLEACHERVQRSLALLGRIAR
                     HIDAQGHDAQSRSAAGDVLRYFDIAAPHHHEDEERHVFPALASHADARVREAVAQLQA
                     DHVQMHALWQRLRAVLLTWRDAEAPPPISAAERALVNDFVTAYERHIPLEESVVYPAA
                     RPLFDERALQTIGAEMAARRRG"
     gene            18623..20479
                     /locus_tag="PKV30_01310"
     CDS             18623..20479
                     /locus_tag="PKV30_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRL28890.1"
                     /translation="MLVLALPGCAVFRDEGAPADPPPASTTDEARAGQVGAVGDSTEA
                     SAAFDIRVEAADDDLRQLIERHNELQRYRPISDLDEAEFARLMALTERDVRNLLGTEG
                     YFNPTVRVRREGAPGGKPTVVIAIEAGEPTTVERVNIEFEGDIASSADADAVAQRTGI
                     VDDWSLDEGRRFTQERWSSAKTGALRSLVERRYPRGRIDYSVADVDAVAARAKLGLRL
                     DSGPLFRLGPATVKGASRYPPELAERLSWLKPGDIYDQKKLVDAQQRLAGSGYYDSAY
                     ISIDPEGDPAATPVTYTVTEAKRHKVQVGIGFSTDGGPRLTLEHRDNTVFGTTWRADT
                     KLHFDQKEPLLQNELTSLPNANGWRWGTLARYMRQDDGTLDTTSQTLRVGRLKTTERY
                     DRNFYLQYDRAKVTGSGTQVVPDALLGDGAAISANYAWTGRYFDSLPLPSRGYGLGAE
                     IGVGMTTVGERKPFVRLQGRWLGIVPVGDGGSRLALRSEAGAIFASDRARLPSTYMFR
                     TGGDATVRGYGYRRIGVPLGGDFVGPGRYMAVGSIEWQRPIMQERFPGLLEHVLFVDV
                     GGVANRVGDLRPNWGVGTGVRLITPVGPMQLDVAYGLQSKEFRLHMNVGFRF"
     gene            20660..>21534
                     /locus_tag="PKV30_01315"
     CDS             20660..>21534
                     /locus_tag="PKV30_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28891.1"
                     /translation="MSDTPESIAARADPSSAGGDFDAQNAAPATRERRRWWRVLLWSL
                     AGLLALLLTTLGGVWIWAGSEGSLATALRWAGAERPLVTDEVTGTVRGGGKVRRLVWD
                     EGGLRVEVHDAEILWTPAALLSRTLQIDQLSASRILIDDQRPKAEAAAGPPTSLALPL
                     NIRVQALRAGELRWAGPPPYTLQDVAGRFAYDGRQHQLELDSARIEGGSYQLRATVTA
                     HEPIGVDVALAGALTAPVPGAEAPVPLTLQARLQGPLTEMQAQADLVAAAPAAGASAP
                     AIPPLPPPANAAPQPP"
BASE COUNT         3510 a         7381 c         7120 g         3523 t
ORIGIN      
        1 cgtagacctt ctcgccgcgc gccaccagct caggcagcgt ccagaccttg ttgggatcat
       61 cggccagggc agccatgcgc ttttgctgat cggccaccca cgcgctgtat tcctgctgcg
      121 gcagcacctt cacgtggatc ggcatgtagg cgtgttcctt gccgcacagc tcggcgcact
      181 gaccgtaaaa gtcgcccgtc tgctcggcgc gaaaccaggt gtcgcgcacg aaaccgggaa
      241 tggcatcctg cttgacgccg aaggccggca ccatccaggc gtggatgacg tcattggcgg
      301 tggtgatgat gcgcaccttc ttgccgaccg gcaccaccag cgggttgtcc accttgagca
      361 ggtaatcgtc gcccgccggc ttgccggcac tggacaaggc gcgctgcgac gaatccaggg
      421 tggacaggaa gccgatgccg gcgccctcgc cattcaggta gtcgtagccc catttccatt
      481 gatagccggt ggccttgatc gtgagatcgg cattgctggt gtccttttgc gccaccacca
      541 ccttggtggc gggcaaagcc atgccgatga cgatgaggaa aggcaccacc gtccaagcca
      601 cctcgacggc gacggattca tggaaggcgg ccggcttcgc accctgcgat ttgcggtgct
      661 tccagatgga atagaacatc accgagaaca cggcgatgaa gatgatggtg cagatgatca
      721 gcatcatcca gtgcagcccg ctttgggctt cggcgatctt ggtcaccggc ggaggcaggt
      781 tgagctggcg cacggccggc ccgcctggca gatcgttgac ctgggccgac gcagcgccag
      841 cccaccatgc cgcgccccat atggccgctt gcgctggcac gccggccagc ttgcttgcaa
      901 tgctcttcat cgtgcccacg ttattcactg cctcaaccaa aaattcactt ccagatcagc
      961 acaccgcttg ccgacccatg gcctgcaacg cactgcgcat ctcccccgcc aaccccggcc
     1021 gccactcggg acgtacgtgg cgccccaagt cgacccgccg cccgtgcgcc atcacctcga
     1081 tcaggccctg gccgctggct ggcggcgcta tgcgcacgtg cgcacagcaa aattccaatc
     1141 tctcgcgcct gccggcgcat tcgcgctcaa cgatcaagcg ctcgccctgc aggcttactg
     1201 tctcgccatc ggccgcgtgc cgggcgtaga tcagaaacgc cgccccgacg gccaccagtt
     1261 caagccccgc gaaccccagc accagcgtcg cacccatcat ccaaaacccg acgccgatgg
     1321 tcaacgacac cgcgctaaga cctgcataca gcaaggccaa ctggcgcggc gtgacactgc
     1381 agttgcgccg cagatgccaa gccagcgcct gatcgctgac ccgagcccac cgatatgccg
     1441 acgtttgctg caaggcgaca gtctccgcac ccaagcgaaa caacatgttc cgcgaaggtg
     1501 gcggcgagtt taaccgactc cccacgaggg tttgacaaat ctcaaacccc gtgcggattc
     1561 tggccacgcc gccgcaacgc ggctctcatg tcgcccagct gaatggcggt ttcgctgctc
     1621 accgcgtggc gcggtggcgc cctgaccgcg tggccgtaga tgacctcgaa ggtgagcgac
     1681 agcgcgccgc cgctggccgg cgccgcctgc gtttcggcca gcgccgcttg caggcgcgcg
     1741 tgccagccgc gcccgcgcag cgcgccaaaa cgctgcaggt gcaggttgcg gcccagctcg
     1801 cgcaactcct gcagcagccg cgcgggcgaa tcgaaggtga gcgtgatgtg ctccatgtcc
     1861 atcaccggct cggcaaagcc ggcggccacc agctgatcgc cccagtcgtg catgtcggtg
     1921 aattcgtgcg ctggcggcgg ccacccgagg cgcgcgtaca gggcgcgcag ctcctgcagc
     1981 gtgtcgggcc cgagacagga gaacatgacg aagccatcca ccgccagcgc gcggtgccaa
     2041 tcgcccatca aggccacggg atcggcgctc tggtgcgcca gcatgttggc ccacaccagc
     2101 tgcaccgacg cggcgggcgg ctgcgccacg tcgatcacct caggctgctg gcgcgaccat
     2161 ttgcgccacc agtcgttcgc tctttttttt gtagcttctg gtgcaagtgg ggcgtgcgcc
     2221 agcacctgaa aacacgctgc atcggggtag cgcgccgcca ccagttgatg accggccagc
     2281 ccaccgcgca ccggctccca gtcggcccag gcgcgcaccg gcaagcgaat cacgtccagc
     2341 cgctcggcca cgcgccgcgc cacctcttcg tgcagccagg ccgagacatg gcgcggctgc
     2401 agcgcccagc gccgggcggc caccgggtcg atggtgggcg gtgattgcga agtcgcgggc
     2461 ggcatggccg ggcagtatat tgagcgcggc cgtgtcgctt ggcccgcctg caaccatgtt
     2521 cacccgcctg ctgcaccgca tcgccgctcc cctgcgcgcg gatgccgcgg cgtggcctgg
     2581 ccagtgcctg atctgccacg cctggccgac cacgaccctg tgcggcgatt gcgtggcccg
     2641 tttcgcgccg gcgcaggcgc gttgcacgac ctgcgcgctg ccggtgccac ccggtgtcac
     2701 cgtctgcggc cagtgcctgc gcaagccgcc gcccatggcg cagtgcctgg ccgccgtcaa
     2761 ctaccgctgg ccgtggtcga tgtgcgtggc gcgctggaag ttcgagggcg atgtcggcct
     2821 gacccggccg ctggccacct tgctgcagtc cacgcccggc gtgcgtgcgg cgctggatgc
     2881 cgccgatctg gtgctgccga tgccgatgtc cgagcagcgc ctggccgaac gtggccacaa
     2941 ccccgcgctg ctgctggcgc gccgcctggc accgctcacc acgcgcgccg gcctgctgct
     3001 gcgcacgcgc gacacgccgc ctcagcgcag cctgccgcgc gccgcgcggc tgaaaaacgt
     3061 gcgcggcgcc ttccaggtcg atccgctggc cgcgccctcg ctcgccgggc ggcgcgtggt
     3121 actggtggac gatgtaatga ccaccggcgc cagcgtgcgc gaagcggctt ccgtgctttc
     3181 agccgccggt gccgcgcaca tcgccgtgct ggtgctggcg cgcaccgacg aaccggcgtg
     3241 acgccccgac gcacaatcgc gcccatgttc cgcatcgtcc tggtcgagcc cgagatcccg
     3301 cccaacaccg gcaacgtgat tcgcctggcg gccaacacgg gctgcgcgct gcacctcatc
     3361 gagccgctcg gcttctcgat ggacgacaag caattgcgcc gcgcgggact cgactaccac
     3421 gagtacgcca gcgtgcgccg ccacgccgac tggcacgcgt tcatgcaggc cgagcagccc
     3481 gatccggcgc gcttgtttgc gctgtcgacg cgcggcacgc ggcacgtgca tgacatctca
     3541 ttcgtggcgg gcgactggct ggtgttcggc gccgaaacgc gcggactccc aagtcatgtt
     3601 cgcaccgcga tccccacggc acagcaattg cgcctgccca tgcgcgccgg acagcgcagc
     3661 ctgaatctgt ccaacgccgt cgcggtgacg gtgttcgaag cctggcgaca aaacggcttt
     3721 gtcggcgcgt agctgcagac aaatcaaaca accgtgcttt tttggccctt cccgacttcg
     3781 gcagggttgg aagttcggcc gccgctcact agactctggt ttttcaccgc tcggtccaca
     3841 tcgccgagtc ccggtttgct gacgccgctc acaaggcggc gcaagacaaa acgcccatga
     3901 tccaatccct caaaccgctg gcacaagccg ccctcaacgt cggccgcttc gtcacgggcc
     3961 cgcgcacggc cacgacggcc gacatggcgc gcatccggcg tgccatgggc gagcaggtgc
     4021 tggattgcga attgcgcgtc gggcgccgcg tgcgcacgca gctggacgcc gccagcaccg
     4081 tgatgcagct gtggctgctg cgcagcgaga tctatcaggc ggtttcagac cagttcggcc
     4141 agcaagaggc gatgcgccgc gtcaagctgc tgacgcctga gttcgagggc ctgctgcccg
     4201 agcgccagct gcgctcggtt tgaccgtggg ccgctcaagc cggccacagc gccgtcacag
     4261 gtagaggcgc gacagcggct ccgccacgca caccggcttt tcctggccct cgcgctcgat
     4321 ggtgcacttc cacgccatct gcaggccgcg ctcgatcggc tcgctgccca gcagggcgat
     4381 gcgcacgcgc agccggctgc ccgagggcac cggccccatg aagcgcacct tgttcagccc
     4441 gtagttgatg cccatgcgca cgtccttgac gctgagggcg cggtcgaaga agcccgccag
     4501 cagcgacaag gtcaggtagc cgtgcgcgat ggtggtgccg aagggccccg ccttggcgcg
     4561 ctcggcatcg acgtggatcc actggtggtc gccggtggcg tcggcaaact ggttgatgtg
     4621 ctgctgcgtc acctcgtacc agtcggtgac ggtgatttcc tgccccgcca gggcggccag
     4681 ttcggccagc gtgttgaagg ttttcatggc gcccaatcta cccgcgcgcc gcgcgcacgg
     4741 ctgtccgccg cgtgactggc cggcgcgcgc tcaagccttg tcgagccagg cggtgatgcc
     4801 ctgccactgc tccagatcgc gcgccacgcg gcccggcccg acatcgaaca gcgtcagccc
     4861 gcgcgccgcc aggtagacgt agttctgcgt cggccgcagc gtgcccagca ccggcagccc
     4921 caggccgtcg gtgaactggc gcaactggtc ggcggcaatg gtgcgctcgt cgacccgcat
     4981 gccgatgagg ccgatgtcgc gccgatcggc gcccttgcct tcgagcaact ggtcgatgaa
     5041 ttcgcgcgtg gcgtagatgt cgaagatgct gggctgcacc ggcaccagca ccttgtcggc
     5101 aatgcgcagc acctctttca gcttgccgcc gtgcaggcca gcgggcgtgt ccaacaccac
     5161 gtgcgtcgtg cccttgggcg ggcgcacgac ggcatcgcgc tgcacctccc agctggttat
     5221 cggcggcgcg gccggtggcc gcaggcccag ccacaggcgt gaggactgct gccgatcgac
     5281 gtcgcccagc atcaccgcgt ggccctggct ggcccagtag ccggccacgt tggtcgccag
     5341 cgtggacttg cccacgccgc ccttggggtt tgcaatcacc accaccggca tgacatgttc
     5401 cttctcttgc ggctcgttcg agcggctatg ttagccgctg cgcgagacgg atcgcgtgct
     5461 gcctgaattc aaagcaaaac agccctccag cgcttgtccc acaagcgcga gaagctatct
     5521 ttttggaagt ttcgatcaca ccaccgcatc ccagatcagc ttgagccccg tcagcagcat
     5581 gccgacgtag atgaagcggt agaacaactg ctggttgatg cgccgcgcaa tgcgcacccc
     5641 gatccacacg cccagcggcg ccagcggcag cagcaccagc gaggtgccca ggttgcgcgc
     5701 gtccagcaac ccgagccacg catacggcac ccacttggcc agattgatga cgaagaagaa
     5761 gtacgccatg gtgccggtga agcgcaccgg cgacagcccg agcgggatca catacgcgtt
     5821 gatcggcggg ccgcccgcgt gcgcgacaaa gctggtgaag cccgatgtgg ccgtgagcag
     5881 cgcgccggcc cagcgcggcg gcggcgcatc gcccgccttg ggcgcgaaca gcaggcgctg
     5941 cgccagaaac agcagcgtga acacgccgac gatgccggcc acccagcgcg cctcgagcaa
     6001 cttgaacgac agcgtgccaa tgacgatgcc cagcagcccc cacggcagca tgaacttcag
     6061 cagcgcgcgg tcgaaatcct tgcgaaacgc cgcgatgccc agcaggtcca tcaccagcag
     6121 caccggcatc aggatcgccg ccgcctgcgg caccgtgacg ctgagcgcca tcagcggcac
     6181 cgccagcgag ccgaagccgg cgccaaagcc gctcttgctc acgcccagca gcagcacggc
     6241 cggcaccgcg accacgtaaa actgccagtc ggtgatcagg ttcatgagtc aaacaggccg
     6301 ctggcgcccg atggacaagc gcgagcagct atcaaaaaca aagtaacgtg atgcgcagcg
     6361 caccacggcg cggccaccga tggtagcggc gcccacccgc gcgcggccac aatacgcgcc
     6421 catggaactg ctgcaaccgc tctcgcccct gtgggccacg cgcctgcaca ttgcgctgga
     6481 gctggccgcc acgctggcgt ttgcgctgtc gggcgtgatc gaggcggcgc gcaagcggct
     6541 cgacgtggtg ggcgtggcgg tggtggcctt tgccgcggcg tttggcggcg gcacgctgcg
     6601 cgatctgctg ctcgattacc ggcccttctt ctgggtgcag cagatcgagt ttttatggat
     6661 cgtgctggcg ctgtgcgctg gcgccatgct gttcatgcgc cagcgccacc tgcggccgac
     6721 cgagcgcgcc atcctgtggc ccgacgccat tggcctgggc ctgttcacag ccatcggcgt
     6781 cgaccgcgcg ctggcgctgg gccagccggc gctggtcgcc gtgatgatgg gcgtggtgac
     6841 cagtgtgttt ggcggggtgc tgcgcgacgt gctggtcaac gaggtgccgc gcgccttgag
     6901 cgaccaccgg ccctacgccc tgtgggcgtt tgccggcggc tggctctacg tcgtgctgct
     6961 cggcgccgcc ctgccgccct ggctggcgct cagcatcacc gtggtggcga cggccgggct
     7021 gcgcgtgctg tcggtgtacc gcgactggca attgcccagc tggcgcgcct gaagtttcag
     7081 ctcagccgcc gacagcgcgg gcacgccact ggcgttgcat caaacgtgcg atcgctcaag
     7141 aaccgttcgc actgagcctg tcgaagtgct ggttccgcgc ggtgcttcga caggctcagc
     7201 ccgaacggat tttctgcaag ccaacccacg caaatgcgtc acctttgggc gagtttggcg
     7261 atcaatccac ccgcgcgccc gactgcttca ccaccggccc ccacttggcc gtctcggcct
     7321 tgacgaaggc ctcgaactcg gccggcttct cggggtgcgc aatggcgccc tgctcggcca
     7381 tctgctgcac cacctcgggc ttggccagca ctttcatggt ggccgcgctc agtttcgcca
     7441 gcacgtcggg cggcaccttg cggttggtga acaggccgaa ccacgaggtc gcgttgtagc
     7501 ccggcacgcc cgactcggcg atggtcggca catccggcag gcccgggtag cgcttgtcgg
     7561 tggtgacggc aatggcgcgc agcttgccgc ctttgacgtg ctgcatcacc gaaggcatgt
     7621 tgtcaaacat gatgtcggtt tgcccgccca gcaaatcggc caccgccggc gcgctgccct
     7681 tgtagggcac gtgcgtcatg tctaccttgg tcatcgcctt gaacagctcg cccgagaggt
     7741 ggatcgaggt gccgctgccc gacgacgcga agttgacttt gcccgggttg gccttggcga
     7801 actcgatcag ctccttcacc gtcttgtacg gtcgcgacgg gtgcgtcacc agcaggttgg
     7861 gcaccatggc cacgcgcgag atgggctgga agtcgcccag cgggctgaag ggcagcttgc
     7921 tgtacagcgc ctcgttgata ccgtgagtgc cgaccgtgcc catcatcagc gtgtagccgt
     7981 cgggcgcggc gcgcgcggcc gcctgcgcgc cgatgttgcc gccggcaccg cccttgttgt
     8041 cgatgacgac gggttggccc agttcgccct ccagcgcctt gcccatgatg cgcgccagga
     8101 tgtcggtggt gccgccggcc gggaagggca cgatcatggt gatcggcttg ttcggataag
     8161 cctgcgccag cgccacgccg ggcagtgcgg cggcaagcgc cagcgcggag gcagcggtca
     8221 gtgcgcggcg gcggggcaag gtgttgcggg tcatgtggtc tcctttgtgg tcaaacggtc
     8281 gacaaaaatc tgtacttaaa acggcgccag cgctttccct acttgcgcaa gaagctatca
     8341 aaataaaagc gaatcggcta cacacccgcc acgcccacgg tcatgccacc gcagacgtac
     8401 agcacctggc cggtgatgaa gccgcttttg gcatccagca gaaaggccac cgcgtgcgcc
     8461 acgtcatacg gcgtgccgac ccgcttgacc ggcacggcat caatgatggc ctgcgtgcgc
     8521 ggcgcgtcgg gcgggttggc cttgtcgaac agggcggtcc ggatcggccc cggcccgatg
     8581 gcgttggcgg tgatgccgtg cgcgcccagc tccagcgccc acacgcgcgt catgccgatc
     8641 aggccggcct tcgtcgcgct gtaggcggtg cgcagctcct tgcccagcgc ggcgcgcgac
     8701 gacatgttga cgatgcgccc aaagcctgct gccctcatgc ccggcagcag cgcctgcatg
     8761 cattgaaagg cgcagcgcag gttgagcgac acggcgaggt cgaactcctg cagcgtctgg
     8821 tgttcggcat cgttcggcac cacgatgccg acgttgttga ccagccgcgt gatcgggcca
     8881 ccggccagcg cctcctgcag cgcccgcgcc gtgtcgtcgg cgctggacaa atcggcgcga
     8941 atgccgtcgc ccacactgtc gatgaccaca gggtcccagc cctcggcgcg gcagcgctcg
     9001 gccgtggccg cgccaatgcc gctggcgccg ccggtgatca gcacgcgctc gcgttgcgtc
     9061 atcaggcttt ttcgaggacc agcgcgatgc cctgccccac gccgatgcac atggtgcaca
     9121 gcgcgtactt gccgccggtg gcgtgcaggc ggttcaccgc cgtggtcgcc agccgcgcgc
     9181 cgctggcacc cagcgggtgg cccagcgcaa tggcgccgcc ccaggcgttg acgcgcgcgt
     9241 cgtcgtcctt caaacccagc atgcgcagca cggccaggcc ctgcgcagca aaggcttcgt
     9301 tgagttcgat gacgtcgatc tggtcgagct tcaggcccgt gagcgccagc actttttcgg
     9361 tggcgggtgc ggggccgatg cccatcacgc gcggcgccac gccggccacc gccatgccga
     9421 ccacgcgcgc tttcggcgtc agcccatatc tgccggccgt ggcttcgtcg gccagcagca
     9481 gcgcgcacgc gccgtcgttg acgccgctgg cgttgccggc cgtcaccgtg ccatcgggcc
     9541 gcaccacgcc cttgagcgaa gccagctttt caaggctggt ttcgcgcggg tgttcgtcgg
     9601 tgtcaaccag cacggcgtcg ccttttttct gcgcaatgct cacgggcgtg atctcgcgcg
     9661 ccaggtggcc ggccttttgc gcggccacgg ccttcatctg gctggccatg gccatcttgt
     9721 cttgcgcctc gcgctcgatc ttgtagtccg tggcgacgtt ctcggcggtc tcgggcatcg
     9781 aatcgacgcc gtactgcgcc ttcatcagct tgttgatgaa gcgccagcca atcgtggtgt
     9841 cgtacaccgc gttggtgcgg ctgaaggcgc tttcggcttt gggcatgacg aagggcgcgc
     9901 ggctcatgct ttcgaccccg cccgctatca tcaatgtagc ttcttcggct ttgatggcgc
     9961 gcgctgccgt gccaatggca tccagccccg aaccgcacag gcggttgatg gtggcgcccg
    10021 gcacgtcgac aggcaggccc gccagcaggc tggccatgtg ggcgacgttg cgattgtcct
    10081 cgccggcctg gttggcgcag ccgtagatca cgtcggtcac cgcggcccag tcgactttcg
    10141 ggttgcgcgc catcagggcc ttgagcggca cggcgcccag gtcgtcggtg cgcacgctgc
    10201 tcaaggcgcc gccgtagcgg ccaaagggcg tgcggatggc atcgacgaca aaggcttgct
    10261 tcattctgag tctcctatta aaaagatagc tgatagcgct tgccagacaa gcgctggagg
    10321 cctattcgac caatatcggt tggcccacca ggcgctgcag ttcgtccaac gccaggccat
    10381 ccacgctgtc aacccagcgc aggccctgcg gcgtgcattc aaaggtcgcc aggtcagtgt
    10441 agatgcgctt gacgcagccg atggccgtga gcggatacgt gcattgggcg acgatcttgc
    10501 tctcgccttt tttggtcaac agatccatca tcacccaggt ctggcgcgcc cccacggcca
    10561 aatccatcgc gccgccgaca gcggggatgg cgcccggctc gccggtgctc cagttggcca
    10621 gatcgccctt ggtggacacc tgaaaagcac ccagcacgca cacgtcgagg tggccgccgc
    10681 gcatcatggc aaagctatcg gcgtggtgaa acaaggcacc gcccggcagc agcgtgacgg
    10741 gctgcttgcc ggcgttgatg aggtcgtagt cttctgtgcc ttcagcgggt gcggggccca
    10801 tgccgagaat gccgttttca gagtgcagga tgacctcgcg cccgggcgga atgtgattgg
    10861 ccaccgcggt cggctgtcca atgcctaaat tgacatacgc gccgtcggga atgtcctgcg
    10921 ccacgcgccg cgccagttcg tctttactgc gtttttgata gctgctcacg cttgctcctc
    10981 ttgcgccagc gatcaaatca cgcggcttgc ttgaagccgc cggcctgcgt cgccacgcgc
    11041 ggcacctgga cgacccggct gacaaagatg ccgggcgtga cgatggcctc ggggtccagc
    11101 tcgcccagct tcaccacgtc gtgcacgctg gccaccgtga acttggccgc catggccacc
    11161 accgggccaa agttgcgcgc cgccatgcgg tacgtcaggt tgccccagcg gtcgccgcgc
    11221 tcggctttca ccagcgccac gtcgccgtaa atcgggtatt cgagcacata gtcccggcca
    11281 ttgatgcggc gcgtttcctt aggcgcacca tcggcgttct tggccagctc ggtgccgaag
    11341 ccggtggggc agaaaaacgc cccgataccg gcgcccgccg cccgcatgcg ctcggccagg
    11401 ttgccttgcg gcaccagctc cagctcgatc ttgccgctgc ggtaagccgc atcgaacaca
    11461 tggctgtcgg cctggcgcgg aaagctgcag atgatcttgc gcacgcggcc ggccttgatc
    11521 agcgcggcga tgccgtgatc gccattgccg gcgttgttgc acaccagggt gaggtcgcgc
    11581 gcgccctgcg cgatcaggcc gtcgatcagt tcggcgggca cgccggcggt gccaaagccg
    11641 ccgatcagca ccgtggcgcc gtcaggcacg tccgccaggg cggcggcgac agtgggggca
    11701 atcttgtcga tcatgccgcc attctcgtcc acgcgccggc acgggcccgg ccgatgcgga
    11761 aaaagcccgt gccgagctca ggcgccgcgg cggatgcgct ggcgcagacg gcgccgaccg
    11821 gccagcgcca tcaggccggc cagcgcgaca aggcccgcgc catgcaccgc cggcaccgac
    11881 gccaggccgc ccgacgccgc cgcgggcgcc tgcaccagcg tggtgaaacg tgcgcgaagc
    11941 gtctcgttga caatgaagcc gaagcggggg ttgaccggcg caacggcggc gatctggaac
    12001 gtggtgaagg ggccagaggc caccagggcg taatcgtcgc agccctcgac gctcgaaccc
    12061 acgggccgcg tgaagttcag caccggcgtg cccaggcccg agacggtggt cagtccatgc
    12121 atctgtgccg ggggtgtgca cgaacctgga ggcggcaaca ggttggccgc agtccagacg
    12181 gcgccaccct ggagcgtgaa ctggaacgag gcgtgctgca tggtgttcag cgtcgtcaac
    12241 tgggtcaggc cggtcgcggc gtcgccgacc actggctggc tgaacgtgta tgtcgccgtg
    12301 ccacccggct ggccgaagaa cgtggctccg aaggtgttgt cggccgtgtt cgtgggcacc
    12361 agattgccgc cggggtcgac attgaaattg aagaacgtgt cggccgcgaa cggattggga
    12421 ttccacagcg tgccctgggt aggcgtcggc gtgtaggtca ccgtcaccgg gggtgtgccg
    12481 gccgcctgcc ccgcggccgt gagctggtag ccgggcggcg gactttgcgt cacggccgag
    12541 aacaccagcg cgtgcgcgct gccggccgca gaaatcagcg ccacgcctgc cgcgctgagc
    12601 cacaatcggt acatcttcat cttcttctcc ctggtgtctc gtgggctgcg atatcgtacc
    12661 agcaccggta tggcgcgcac cgcgccgaga accaccccgc accgccatgt tcgcccccac
    12721 ccaacaagac gtccgccgct ttttctgtag cgcctgggcc aagcagcaat cgggccagcc
    12781 gatggaaacc atcgaaacgc tggccgccgg ctggatcgcc gagcaccccg agtggcacgc
    12841 cgatttcagc gatgccgagg ctgccgtagc gcgcgattac ggcgatggcg cggtgggcgg
    12901 caacccgttc ttgcatcttt caatgcacct gtcgatcagc gagcagtgca gcatcgacca
    12961 gccgcgcggc atccgtcagg cggtggaact tctggccgcg cggcgtggtg atctgcacgc
    13021 ggcgcaccac gaggtgatgg agtgcctggg ccagatgctg gccgacgcgc acgccagcgg
    13081 ccggccgccc gacggcgacg gttacatcgc ctgcgtgcag cggcgcgcca cgcgcgatgc
    13141 gctttgatct gcttcttttt ttatagctgc tcgcgcgctc cagacaagcg ccagggccaa
    13201 aaattccttt aaaaaaacgc tgccagaaag aacaaagccc gcatgcagcg ggctttttaa
    13261 aaccgggccg atcgcgcggc aggatcaacg aaagcgcgac tcgggcaccg ttgtcatgtc
    13321 gccttgcagg ctggcgatgt atttggccat ggccttcagt tcggcatttg aaaactgctt
    13381 ggccacgccg gccatgaccg gattgccgcg gccccacgtg tggtgcgtgt tgtcgcggta
    13441 ggacttgagc gccacgtaca ggtagtcggc gtattggccg ccgatcttgg ggtatgtcgg
    13501 gtcgatcggc ttcgagaagt tgtcgccgtg gcaggacacg caggcgccct tttgcagcaa
    13561 ctgcgccacc tgggcgctgg gttcgcgcgc ggcgggcaac tcgggcgcgc cctctttgac
    13621 gccgtgttgg gcgtagtagg cggccacgtc ggcaatgtcc tgctcgctca tcgtggtggc
    13681 gatgccgcgc atgctggggt gcttgcggtc gcccttcttg taggcctcca gcgcggcggc
    13741 gatgtacttg gcgttctggc ccgagatctt gggcacgtgg tagacctcgg gaaagctcga
    13801 cttgtagccg atgatgccgt ggcagccgat gcacatggcg atcttgcctt cgccggcctt
    13861 ggcatcgccc gtgacctggg cctgggccgg catggcggcg cccagggcga caacgatggc
    13921 agcgaacatc ggggacagag tcgttttcat tttgcgcgca caattcaaat tggtttgatc
    13981 caggtcagcg gcgattgtaa tcaagcccgc ttgccccgca gtccgctgcc cgccctgttg
    14041 tccacattgc cacccgttcg cttcgacatg aaattccaag gctccgaaaa ctacgtcgcc
    14101 acgcaagacc tgatgctggc cgtcaacgcc gccatcacgc tcaagcgccc gctgctcgtc
    14161 aagggcgagc cgggcaccgg caagaccatg ctggccgagg aagtggccga ggcgctgaag
    14221 ctgccgctgc tgcagtggca catcaagagc accaccaagg cgcagcaagg cctgtacgaa
    14281 tacgacgccg tgagccgcct gcgcgacagc cagctgggcg acgacaaggt caaggacatc
    14341 cacaactaca tcgtcagggg cgtgctgtgg caggccttca cggccgacga accggtggcg
    14401 ctgttgatcg acgagatcga caaggccgac atcgaattcc ccaacgacct gctgcgcgag
    14461 ctggatcgca tggagttcca ctgctatgaa acgcgcgaaa ctgtgcgcgc caagcaccgg
    14521 ccggtggtct tcatcacgtc gaacaacgag aaggagctgc ccgacgcctt cctgcgccgc
    14581 tgcttctttc actacatcaa gttccccgag cccgagacga tgaagcagat cgtcgacgtg
    14641 cacttcccga atttgaagaa ggaactgctc gccgcggcga tgaaggtgtt ctacgacgtg
    14701 cgcggtctgc cgggtttgaa gaaaaagccg tcgaccagcg aactgctcga ctggctgaaa
    14761 ctgctggtgg ccgaagacat tccgctcgaa gcactgcaaa gcgccgacag caaggtcagc
    14821 gtgccgccgc tggtcggcgc actgctcaag aacgagcagg acgtgacgct gttcgagaag
    14881 ctggtgttca tgaaccagcg caaccgctga ggtcgccatg aacccgctgt tgcttgaagg
    14941 cctggcggat gcgctgggct ttgtcggtgg cgcgctggtc ggcttcctgc tggcgcggct
    15001 ggccgggttc gacttgtttg ccgaaggcta tggcgcgtcg agcatggccg ccatcgtggc
    15061 ggtgggagta ggcgccggct tgggccgcgc cgtcacccgc cgctggcggc tgcgtcgcga
    15121 agccgcgcga cgggccgctt ctgcaccctc ttccaagtcc tgacatgagc tttgtcgaat
    15181 ccgttgtgct gcgcgaccat ggcgtgcgcc tggaaccgct ggcgctgtct cacgaagacg
    15241 gcttgcgcgc cgccgccgcc gacggcgaac tgtggaagct gcgcgtgacc agcgtgccgg
    15301 cgcctcagga gacacgcgcc tacatcgaca ccgccttgaa aatgcgcgaa gaaggccacc
    15361 gcttcgcctt tgccgtgctt gacgacgcca ccggcacggt gctgggcagc accagctacc
    15421 acgacatcgt gccggcgctg cgccgagtgg agattggcta cacctggtac cgccagagcg
    15481 tgcagcgcag ccacgtcaac accacgtgca agctgctcat gatgggccac gccttcgata
    15541 cgctgcagtg ccccgtggtc ggctggcgca ccgacaacta caactttgcg tcgcaaaagg
    15601 ccatcgaacg gctgggcgcg cgcaaggacg gcgtgatccg ccaccacgcg ctgcgccgcg
    15661 acggcacggt gcgcgacaca gtcatgtaca gcatgacgga cggcgagtgg cctgaagcga
    15721 aggcgcaatt gctgtatttg cgcgcgcgcc gcggctgaac gccagcggcc ttattggccg
    15781 cgctcggcgc cgaattccga atccgacagc agccgctcgt acaagggcgc gacgagcgcg
    15841 gcgctgtctt catcgatcag gcagtcgagc aggcgcgcga tggaagcgcg cggcaacagg
    15901 cgggcgatct gctcggccca gggcagcgcc tcggtgtcgc cccaggcgcg caccgccagc
    15961 ccgatggcgc gcgccagcag ggccagcgtg tcgtcctcgg cctgcgtgtc ggcgctgtcc
    16021 ttgaccagtt gctcggccgc ttcaccgcca atctcgatca gcatcacgcc ggcctgcgcc
    16081 cgcgcggcct gcagcgagtc ttcgtccggc acgcgtgtga tgccaaatcc cgactggttg
    16141 gccgacgtcg cggccacgcc acccagcggc ggcagagtcg gcgcaaactc gggcggcacg
    16201 tgcggccacg cggtgggctc tgccgcttcg atcaggccgg cagccaccag gcggcgcaga
    16261 tgggccggcg tgtcggggcc gaacacctcg gtcatggcca ccacgttgcg ctcaccagtg
    16321 cacagcagca cgatctggcg gtcgcgcaag gccatgtggc cacgcagggc agccagcgct
    16381 tgccggccgg cttcggtctt gcggtacacc aggctgcgct cttcagcgtc tggcccgatg
    16441 ggtgaggggt gggcgctacg gatgggtgtc atggtgggct caatgcgtac ttcggtagac
    16501 gggaaaacgg gtagccggct ttacgatccc ttagtcaatt ttggtcagta tgcacaaagt
    16561 atgcattttc cgcgccaccc gtttgaaggg gcggttcggc ggcgtgcgtc cgctgggtga
    16621 ccgccgtttt tcgtcacccg gcccataatg gttgcgaggc ccgaccatgc tgatcgactt
    16681 tttctacacc ttgcgcgccg ccaaattgcc ggtgtcggtc aaggaattcc tgacactgct
    16741 cgaagcgctg caggccgacg tggtcggtcc gcgccaaccc gacgcctgct cgatcgacga
    16801 cttttactac ctggcgcgca ccgcgctggt caaggacgag aagcacttcg acaagttcga
    16861 ccgcgcgttt gccgcctact tcaagggcgt cgagcacatc gctgacttcc gcaaggagat
    16921 cccggctgag tggctgcgca agattcttga aaaagagctg acgcccgagc aaaaagccgc
    16981 catcgagaag atgggctggg acgagctgat ggaaacgctc aagaagcggc tcgacgagca
    17041 gaaagagcgc cacgaaggcg gcagcaagtg gatcggcacc ggcggcacct cgccctttgg
    17101 ccatggcggc tacaacccgc agggcatccg cattggcggc aaagggggca acaagagcgc
    17161 cgtgaaggtg tgggaccagc gcgcctaccg cgactacgac gacagccagg aactgggcac
    17221 gcgcaacatc aaggtcgcgc tgcgccggct gcgcaagttc gcgcgcacgg gcagcgagtt
    17281 cgagttggac ttgccagaca ccattcgctc aacggcggcc aatgccggat ggctcgacat
    17341 caagatggtg cccgagcgcc acaacagcgt gaaggtgctg ctgctgatgg acgtgggcgg
    17401 caccatggac gagcacatcg cccgcgtgga agagctgttt tcagccacca aggcggagat
    17461 gaaacatctg gagttctatt acttccacaa ctgcgtgtac gacttcatgt ggaagaacaa
    17521 ccggcgccgc tttgccgaga agttcgccac ctgggacatc atccgcaagt acaacaagga
    17581 ctacaagctc atcttcgtcg gcgacgcgac gatgagcccg tacgaaatcc tgcaaccggg
    17641 cggcagcgtc gagtacaaca acgaggaacc gggcgccgaa tggctgcagc gcctgacgca
    17701 cgccttcccc aagtttgcct ggatcaaccc cgagccgcag ggcgtgtggc agtaccgcca
    17761 gagcattgcc atcatccagc agttgatgag tcatcgcatg tttccgctca cgctcaaggg
    17821 gctggaggat gcgatgcgga tgctttcaaa ataatagctg ctcgcgcttg ataaacaagc
    17881 gctggagccg cttttcattc aaattcggcg attcagccgc gccgccgcgc cgccatctcg
    17941 gcgccgatgg tctgcaacgc acgctcgtcg aacagcgggc gcgcggccgg gtagaccacc
    18001 gattcttcca gcggaatatg ccgctcgtac gcggtcacga agtcgttcac cagcgcacgc
    18061 tctgccgcgc tgatcggcgg cggcgcttcg gcatcgcgcc aggtcagcag cacggcgcgc
    18121 aggcgctgcc acagcgcgtg catctgcacg tgatcggcct gcagctgagc caccgcctcg
    18181 cgcacgcggg cgtccgcgtg cgaggccaga gccgggaaca catgccgctc ctcgtcctca
    18241 tggtggtgcg gggcggcgat gtcgaaatag cgcagcacgt cgccggccgc tgaacgcgac
    18301 tgggcatcgt gcccttgggc atcgatgtgc cgggcgatgc gcccgagcag cgccaggctg
    18361 cgctgcacgc gttcgtgaca ggcctcgagc atttcaaacg gctgctcgaa gccgacggcg
    18421 ggcgtgttga agcccggaag attgacgcca gcttgtgcca tggccgcatt gtcggcccgc
    18481 ccgcaaagtc ccttggctgg cacgggcgtt ggccgtcggt atcggctgga aaaaggggct
    18541 ttgtccgaca aggtcgccga ctaaacttga agcggtgagc cttgtgaccg tttccccccg
    18601 atcgccatcc gctgccgtgt tgatgttggt gttggcgctg ccgggctgcg ccgtcttccg
    18661 cgacgagggg gcgccagccg acccgccgcc cgccagcacc accgacgaag cgcgcgctgg
    18721 ccaggtcggt gccgtgggcg actccaccga ggccagcgcc gcgttcgaca tccgcgtcga
    18781 ggcggcggac gacgacttgc gccaactaat cgagcgccac aacgagctgc agcgctaccg
    18841 ccccatcagc gatctggacg aggccgagtt cgcgcgcctg atggcgctga ccgagcgcga
    18901 cgtgcgcaac ctgctgggca ccgagggcta cttcaacccg accgtgcgcg tgcgccgcga
    18961 aggcgcgccc ggcggcaaac cgaccgtggt gatcgccatc gaggccggcg agccgaccac
    19021 cgtcgagcgc gtcaacatcg agttcgaagg cgacatcgcc agcagcgccg atgccgacgc
    19081 cgtagcgcag cgcaccggca tcgtggacga ctggagcctg gatgaaggcc gccgcttcac
    19141 gcaagagcgc tggtcaagcg ccaagaccgg cgcgctgcgc agcctggtcg agcggcgcta
    19201 tccgcgcggg cgcatcgact acagcgtggc cgatgtcgat gccgtggcgg cgcgcgccaa
    19261 gctggggctg cggctggatt cggggccgct gtttcgcctt gggccggcca ccgtcaaagg
    19321 cgccagccgc tacccgcccg agctggccga gcgcctgtcg tggctcaagc cgggcgacat
    19381 ctacgaccag aagaagctgg tcgatgccca gcagcggctg gccggcagcg gctattacga
    19441 ctcggcctac atcagcatcg accccgaagg cgacccggcg gccacgccgg tgacctacac
    19501 cgtcaccgag gccaagcgcc acaaggtgca ggtgggcatc ggcttcagca ccgacggcgg
    19561 cccgcgcctg acgctggagc accgcgacaa cacggtcttc ggcaccacct ggcgtgccga
    19621 caccaagctg cacttcgacc agaaggagcc gctgctgcag aacgagctga cctcgctgcc
    19681 gaatgccaac ggctggcgct ggggcacgct ggcgcgctat atgcggcagg acgacggcac
    19741 gctcgacacc acttcgcaaa ccctgcgcgt tggccgcctg aagacgaccg agcgctacga
    19801 ccgcaacttc tacctgcagt acgaccgcgc caaagtcacc ggctcgggca cgcaggtggt
    19861 gcccgacgca ctgctgggcg acggcgccgc catcagcgcc aactacgcgt ggacggggcg
    19921 ctacttcgac agcctgccgc tgccctcgcg gggttacggc ctgggtgccg aaatcggcgt
    19981 cggcatgacc acggtgggcg agcgcaagcc cttcgtgcgc ctgcaggggc gctggctggg
    20041 catcgtgccg gtgggcgatg gcggcagccg cctggcgctg cgcagcgagg ccggcgccat
    20101 cttcgcgtcg gaccgggcgc gcctgccctc cacctacatg ttccgcaccg gcggcgatgc
    20161 cacggtgcgc ggttatggct accggcgcat cggcgtgccg ctgggcggcg actttgtcgg
    20221 cccgggccgc tacatggcgg tgggcagcat cgaatggcag cggcccatca tgcaggagcg
    20281 ctttccgggc ctgctggagc acgtgctgtt cgtcgacgtg ggcggcgtgg ccaaccgcgt
    20341 gggcgacttg cggcccaact ggggcgtggg caccggcgtg cggctgatca cgccggtggg
    20401 gccgatgcag ctggacgtgg cgtatgggct gcagtcgaag gagtttcggc tgcacatgaa
    20461 cgtgggcttt aggttttgac gtcccccctg agtcgccttt ggcgcctttc ccccaggggg
    20521 acaacgccag tggccgggcc aagcccgttc cacggcgttc cctcgtggcc tgctccgcgg
    20581 cgttttgaat tggtctccct cccccgctgg gggagggttg gggtgggggc caccccggcg
    20641 cccacgcact gagattcaga tgtcggatac tcctgaatcc atagctgctc gcgctgatcc
    20701 atcaagcgcg ggaggcgatt ttgatgctca aaacgccgcc ccggcgacgc gtgagcgccg
    20761 ccgctggtgg cgcgtgctgc tgtggtcgct ggcggggctg ctggcgctgc tgctgaccac
    20821 gctcggcggc gtgtggatct gggcgggcag cgaaggctcc ctcgccaccg cgctgcgatg
    20881 ggccggcgcc gagcgaccgc tggtgaccga cgaagtcacc ggcacggtgc gcggcggcgg
    20941 caaggtgcgg cggctggtgt gggacgaagg cgggctgcgc gtggaagtgc acgacgccga
    21001 gatcctgtgg acgcctgccg cgctgctgtc gcgcacgctg cagatcgatc agttgtcggc
    21061 cagccgcatc ctgatcgacg atcagcgccc caaggccgaa gccgccgccg ggccgcccac
    21121 gtcgctggcg ctgcctttaa acatccgcgt ccaggcgctg cgcgccggcg agctgcgctg
    21181 ggccgggccg ccgccctaca cgctgcagga tgtggccggc cgctttgcct acgacggccg
    21241 ccagcaccag ctggagctcg acagcgcgcg catcgaaggc ggcagctacc agttgcgcgc
    21301 caccgtcacg gcgcacgagc ccatcggcgt cgatgtggcg ctggccggcg cgctcaccgc
    21361 acccgtgccc ggcgctgagg cgccggtgcc gctgacgctg caggcgcgcc tgcaagggcc
    21421 gttgaccgaa atgcaggcgc aggccgatct ggtggcggcc gcgccggctg cgggcgcgtc
    21481 ggcgccggcc atcccgccgc tgccaccgcc ggccaacgcc gcgccgcagc cgcc
//
LOCUS       DAOMVO010000011        20933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_443_length_20933_cov_15.695948, whole genome shotgun sequence.
ACCESSION   DAOMVO010000011 DAOMVO010000000
VERSION     DAOMVO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 20933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20933
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_443_length_20933_cov_15.695948"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKV30_01320"
     CDS             <1..985
                     /locus_tag="PKV30_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRL28892.1"
                     /translation="GTLVVLVYTLYGGMWSVAMTDFVQMIVITLGLLAIAWFAAGMAG
                     GADKVIDYAAREGKFRFWPTGGAKEWLFFIGAAITMMLGSIPQQDVFQRVMSSKDVQT
                     AQRGPVIGGVLYILFAFVPMFVVVAALLVMPEAASLLADDPQKVLPRLVMDHMPMALQ
                     IAFFGALLSAIMSTASATLLAPSTTFVENILRNLKPGMSDAETLKAMRISVLVFTACV
                     LVYAITMQGSSIYELVAGAYQVPLVGAFVPLAFGVYWKRATTQGALLAVVLGIGVWLL
                     FVASPMLNQGFPQQLAGVLAAVAGMVFGSLAPQWLPDHKDHVHHYEGSAAA"
     gene            1105..1473
                     /locus_tag="PKV30_01325"
     CDS             1105..1473
                     /locus_tag="PKV30_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRL28893.1"
                     /translation="MPIYAYKCAACGFAKDILQKMSDAPLTECPSCGQPAFSKQLTAP
                     GFQLKGSGWYVTDFRDGQKKSGGGQEPAAEGAKPESGKTEGAAPASAPASAPAPASSP
                     APAAPPPAGGGGSSSSSSGS"
     gene            1489..2250
                     /locus_tag="PKV30_01330"
     CDS             1489..2250
                     /locus_tag="PKV30_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRL28894.1"
                     /translation="MMSRLRQYFITGLLVWLPMGVTVWVLLWLVGILDGIFLGVLAAA
                     EAVTPGLTPLADQLRRIPGLGVILVAIVIFGTGVFVANMFGQWAVRQWDKLMTRIPVV
                     RSIYSSVKQVSDTLFSGSGQAFSRALLVQYPRQGAWTIAFLTGRPGGEVARHLDGDYL
                     SVYVPTTPNPTSGFFLMVPRADVIELAMSVDEALKYVISMGVVVPPLREPRPGEPVTA
                     QVPGVTGPKAAAPAAEALPPVNHPMVDPAIARPEL"
     gene            2265..4058
                     /gene="aspS"
                     /locus_tag="PKV30_01335"
     CDS             2265..4058
                     /gene="aspS"
                     /locus_tag="PKV30_01335"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRL28895.1"
                     /translation="MSMRSHYCGLVTEALIGQTVSLCGWVNRRRDHGGVIFVDLRDRE
                     GYVQVVCDPDRPEMFKVAEDLRNEFCVQVKGVVRARPEGTTNDNLKSGKIEVLCHELT
                     VLNPSVTPPFQLDDENLSETTRLTHRVLDLRRPYMQNNLMLRYKVAMEVRKFLDEHGF
                     IDIETPMLTKSTPEGARDYLVPSRVHEGHFFALPQSPQLFKQLLMVAGYDRYYQITKC
                     FRDEDLRADRQPEFTQIDIETSFLGEQEIRDLFQRMITHVFQKTMNVELGEFPVMPYA
                     EAMHKYGSDKPDLRVKLEFTELTDVMKDVDFKVFSGPATTPGGRVVALRVPGGSEISR
                     SEIDGYTEFVKIYGAKGLAWIKVNDAAAGREGLQSPIVKNLHDAAIAAILERTGAQNG
                     DILFFGADKAKVVNDAIGALRLKIGHGDLAKKSGLFEDSWAPLWVVDFPMFEFDEDDH
                     RWNAVHHPFTAPKDGHEDYMDSDPGKCVAKAYDMVLNGWELGGGSVRIHRADVQSKVF
                     DALKISPEDAQLKFGFLLDALQYGAPPHGGLAFGLDRLVTLMTKAESIRDVIAFPKTQ
                     RAQDLLTQAPSPVDEKQLRELHIRLRNATPA"
     gene            4144..4473
                     /locus_tag="PKV30_01340"
     CDS             4144..4473
                     /locus_tag="PKV30_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HRL28896.1"
                     /translation="MSYTSDLVDSATETKDQLVSNLRRVVSDAEDLLAATAGQTDSKI
                     TELRARAKENLSMAREKLADADAVVRARARQAAAVTDEYVHDNPWSSIGAAAALGILI
                     GVLLGRR"
     gene            4494..4877
                     /locus_tag="PKV30_01345"
     CDS             4494..4877
                     /locus_tag="PKV30_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRL28897.1"
                     /translation="MSLPSSDASTAARVRGLLADVIELAQVRFELFTVEAREELARLA
                     GMAVMGALAVVFVSFGLIFLAIFLTVLLWDTHRVLALGIFTSLFLGGGLVLALLAWNK
                     AKQGFRMFGATRSELQRDQDRLRTS"
     gene            4874..5200
                     /locus_tag="PKV30_01350"
     CDS             4874..5200
                     /locus_tag="PKV30_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HRL28898.1"
                     /translation="MKDDPSLNLATRREQLRLRSAQLREQIAVRSQVFQPAFRATDRV
                     RGGVRSVRGVRDNRALLMLGGAALAGALLVRPRAALQLGIRAWSGWQVYRRMRPLVNS
                     VLRQLG"
     gene            5256..5726
                     /gene="nudB"
                     /locus_tag="PKV30_01355"
     CDS             5256..5726
                     /gene="nudB"
                     /locus_tag="PKV30_01355"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HRL28899.1"
                     /translation="MLKPFKIPESVLVVVHTAALDVLLIRRADAGADEFWQSVTGSKD
                     ALDEPLAHTAAREVGEETGIDALGPDCALTDWRIENLYDIYPRWQARYAPGVTRNREH
                     VFGLCVPRDVPVLLNPREHSDFVWLPYREAADRCFSPSNAEACLLLPRFSKVTA"
     gene            5723..6487
                     /locus_tag="PKV30_01360"
     CDS             5723..6487
                     /locus_tag="PKV30_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRL28900.1"
                     /translation="MTADSSFGEPHDILRVATYNIHKGVQGVGPTRRLEIHNLALAIE
                     TLDADIVCLQEVRQGNRKEASYFTGWPEQPQAEFLAPEGYEAVYRSNAVTKHGEHGNA
                     LLSRWPVIECRHEDMSDHRFEQRGLLHAKLLVAGVTVHAIVVHFGLIPSSRVRQTAHL
                     KAYIAREIPLDEPVVVAGDFNDWGTRVRRLLHVDSLHAFEGPRTLTYPSRYPVAQLDH
                     IYARGLRPLSLAAPRGRVWSRMSDHLPLIAEFNLPQ"
     gene            6746..7471
                     /gene="folE"
                     /locus_tag="PKV30_01365"
     CDS             6746..7471
                     /gene="folE"
                     /locus_tag="PKV30_01365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRL28901.1"
                     /translation="MKASPDLPAAADASDEGTPVSVKIRERLQAARRRFNANDNIADF
                     IEPGELEKLLDEVTAKMEGVLDSMVIDTGRDHNTANTARRVAKMYVNEVFKGRYVKPP
                     EITEFPNAERLNELMIVGPITVRSACSHHFCPVIGKLWIGVMPNERTNVIGLSKYARL
                     AEWIMGRPQIQEEAVVQLADLIQQKTKPDGLALVMEATHFCMAWRGVKDMDSKMINSV
                     MRGVFLKDMNLRREFLSLIPQKT"
     gene            7712..8134
                     /locus_tag="PKV30_01370"
     CDS             7712..8134
                     /locus_tag="PKV30_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HRL28902.1"
                     /translation="MLVRLLYASRAVDTTPAAIEAICASARQHNAEQGVTGVLVYGGG
                     IFMQAIEGGRQTISDLYGVIQRDGRHQDVVLLHYEEIAERRFGGWTMGLVNTSRINPS
                     TLLKYSERAELNPYAVSGRVSMALIDELMATASIVGHT"
     gene            complement(8160..9848)
                     /locus_tag="PKV30_01375"
     CDS             complement(8160..9848)
                     /locus_tag="PKV30_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRL28903.1"
                     /translation="MHRALARRLPFLNWPRPSGALLRGEALAGLTVGLMVIPQGVAYA
                     ALAGMPVVTGIYASMLPALVAALFSASPRLSVGPTALSSLLVAASLTGLAAPGSAEWV
                     NLAVWLALLTGALQIGLGAVQAGWLLNLVTAPVLMAFTQGAAVLIIFSQLPALLGLPK
                     DGDWAARLTGAHWASAAFGIGALALLVVARRLKPSFPGVLVVVLVAAAVSAFTPYAAQ
                     GGAVVGPLPQGLPGLFVPHWPGWATLGQLVMPTLIITLVSFLETASSAKVDSAARGAR
                     WDQNQDLIGQGLAKLASGLSGAFPTSSSFSRSALNLYAGAKTGWASIFSAVVVGLALL
                     VFTPVLRHVPQAVLAAIVVATVYGLLKPREFGRLWQVSRLEAVIAAVTFVVTLLAAPA
                     LYWGVLAGVLMSLSHFLYQHLHPRILEVGEHPDGSLRSRTLWRLPPIAPRVLALRMDA
                     ALDFATAAAFERAVQDALTLNPDTRHLALFMQPVNRIDATGVETFGKLAAHLMGQHIA
                     FHLVGLKLPVEQPLARAGLLNARAGLRLHRTDAEALSALRSTGDESADLAAAMI"
     gene            9977..10591
                     /locus_tag="PKV30_01380"
     CDS             9977..10591
                     /locus_tag="PKV30_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRL28904.1"
                     /translation="MNRLALVALFALLPAVVGAQAIRCKDPATGKILYTDQPCKGGEL
                     VVPQRSEADIARDAANAAAAREREYDRALQQREERALQREQLRAQQAALPPPAPPAET
                     DACRAARAEAAFRAGSFSASAEEIRTARYNAALACGQQPPADIVVVQPPEPLYPVRRP
                     PRFDDPRRPHRPGDGFGMPRPAPRPQYEPGTEPVPVLVRPPSAR"
     gene            complement(10603..11940)
                     /locus_tag="PKV30_01385"
     CDS             complement(10603..11940)
                     /locus_tag="PKV30_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRL28905.1"
                     /translation="MTQKTSRPNIIFIVADDLGFADLGCYGGRDAAFGPVSPVLDQLA
                     AAGLKFTQGYSNSPVCSPTRFGLMTARYQYRLRGAAEEPINSKSKGSTTLGLPPEHPT
                     LPSLLKGAGYRTALMGKWHLGYPPVFGPLMSGYEEFFGPISGGVDYFTHCSNNGTHDL
                     YLGDAEHHEEGYLTDLITERSVDWLQRMAPGARGGTPFFLSVHYTAPHWPWETRGDAA
                     LAEELKDNLFHLHGGNIHTYRRMIHHMDEGIGQLVETLRAEGLLDNTLIVFTSDNGGE
                     RFSDNWPLVGGKMDLTEGGIRVPWIAHWPARIAAGGVSPQACMTMDWSATMLDAAGVA
                     AHPDYPLDGVSLLPVLGDVAQRFARPLFWRMNHRGQRAHRDGDWKYLRVDGHDYLFNL
                     SQDERERANLAARESERLAALRDAWEAWNASMPAIPPDATVSLGYGAKDMPQR"
     gene            complement(11997..12974)
                     /locus_tag="PKV30_01390"
     CDS             complement(11997..12974)
                     /locus_tag="PKV30_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL28906.1"
                     /translation="MTATPFFTSARRTLSAATFSLVASAASAQGFPTKPITFVNSFPA
                     GGPSDMIARSVAEVLQQRFKQPVVVENKPGAAGNIGTAQVARSAPDGHTVLIGIDTIF
                     TVNPHIYKGLPFKPGDFKPLMVMASSGLLVGAHPSTGFKTLADLVNAGKAQGVTFSSA
                     GSGSPGHLAAEVFHEATGMQITHVPYKGNTPAVTAVLSGEVNGGVLATPGLLPHVKAG
                     KVTALAVTSRQRSTLAPEVPTVAEAGHKGLEQEVLYIAMVPAATPDAVVQTLQKAFAD
                     ALAEPQVKQRLANLDLFYEGLSGAAAAKRLGDLSTRYGRVIQATGMKVE"
     gene            complement(13049..14011)
                     /locus_tag="PKV30_01395"
     CDS             complement(13049..14011)
                     /locus_tag="PKV30_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HRL28907.1"
                     /translation="MHRRHLIQAASAASLGWLTSRAHAQADTTLRLVVPYAPGGSSDR
                     AARLVADKLGPKLGQTVVVENRTGAGGRLAAQQLKNAPASQNVLMLANPAIMVVAPLV
                     FKDNGYDADKDFQPVSQVSDYEFGLAVAQAVPVKQLSHLLAWLRANPAQANFGVPATG
                     SLPHFFALMTAEKADVKGQVVGYRGSGPLITDLIGGQVPVAFDTLDTLLPQHEGGKLR
                     ILASSGAQRSSFAKDIPTYRESGLDLVATGWNAFFAPAAMPREQVQRLSAAIRDVMND
                     PDTRRRFADAKMTPVASTAAQTAAMLKAYRAQWAPVVRKSGYQP"
     gene            14105..14596
                     /locus_tag="PKV30_01400"
     CDS             14105..14596
                     /locus_tag="PKV30_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRL28908.1"
                     /translation="MATNRLTEPKTLDDMLLYVLWQLQAAARGPVVRVCEAEFGITRR
                     EWRMLAQLGQGEGLASSALAERAGLDRAQTSRAVGGLVGKRLLARTPRPGDRREVLLH
                     LTDEGRAVYNALLPRIAAINRDLLSVLAPAEVEVLDGLLRRLNAQALRVGAVGDDVVP
                     KFE"
     gene            complement(14740..15117)
                     /locus_tag="PKV30_01405"
     CDS             complement(14740..15117)
                     /locus_tag="PKV30_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28909.1"
                     /translation="MNSYSTVEKIADKAEVATDKMADKAERSVESARLYANEALDKAD
                     AKVRSLREDVKPAIDAISSRVHDMAARGKAAAAETSAQTREKLNEYTDKTSAYVAEQP
                     LKSMAIAAAAGAALALLMGRRRG"
     gene            complement(15246..16724)
                     /locus_tag="PKV30_01410"
     CDS             complement(15246..16724)
                     /locus_tag="PKV30_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HRL28910.1"
                     /translation="MLDPSAPTQDDAQRARKPLSLRITLVIAALCAIAIMWLNEDTYH
                     RTTQTLVQIQHGHATRSTINTVVRRLVEAESGQRGFILTGDRAYLAPYDAALKDIDVL
                     LKRLGANVPRAMNESEAMQAFRHLLSQKIGEMALTVRLRQEERAEVVNFVMTSNVGLE
                     QMRAFQLQGDALLAQADAVVAANRAELYRLLNVSRFGLAAGVLAAFFAFFLYVNQTRT
                     LRETDARQKQLLEAERDALESQVRERTARLTELATYLQQAVEDERAHLARELHDELGA
                     MLTAAKLNVARIKSKLPKDADELAERLKHLTETLNQGIALKRRIIEDLRPSSLSNLGL
                     VASLEILTREFADRSGVEIETFLEPVELDEVSELTIYRMVQEALTNIGKYANATKVIV
                     RVKNYTYHAEVTVQDNGVGFDATRLPQASHGLVGMRHRVEASGGRLDINSRAGGGGSR
                     ITGTIPRAVSKPQSALAESVGAMSSDSYKAPSPQTTAHDAAI"
     gene            16799..17431
                     /locus_tag="PKV30_01415"
     CDS             16799..17431
                     /locus_tag="PKV30_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL28911.1"
                     /translation="MIRIGIVDDHAIVRSGLKQFFSEHVDLRVVGEAASGREAIDLVR
                     TTEMDVLVMDLSMPGQSGIDALAMIRAKAPDVGILILSGYPEEHYAMNLIRQGASGYL
                     NKECDPAEIVEAIRTIALGKRYITPTVAELLAQQLNRKNDALPHEQLSEREFQVFLKL
                     AKGQTAGDIADSLSLSVKTVSTYRTRLMEKMSLSSNSDLTYYALKNKLID"
     gene            complement(17524..17913)
                     /locus_tag="PKV30_01420"
     CDS             complement(17524..17913)
                     /locus_tag="PKV30_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL28912.1"
                     /translation="MPGPSPVRAYFVEDNPTIRENLIATLEELGGVAPIGYAETEQQG
                     SEWLTNHSANWDLAIVDLFLKQGSGLGVLKACRERNPEQKVVVLTNYATPDIRVRCHQ
                     LGVDAVFDKSNEIEALIDFCLEMKGNA"
     gene            complement(18055..18813)
                     /locus_tag="PKV30_01425"
     CDS             complement(18055..18813)
                     /locus_tag="PKV30_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRL28913.1"
                     /translation="MDDRQLLRYSRHLLLDDIGIEGQQRLLDSHALIVGAGGLGSPVA
                     LYLASAGVGQITMVDDDTVDLTNLQRQIMHTEARVGQPKVASAEAAIGALNPGVRVRT
                     VAQRADEAQLTELAAAADIVLDCTDNFATRQALNRACVATGRPLVSGAAIGFDGQISV
                     YDPRRAASPCYACVFPPTQAVEEARCATMGVFAPLVGIIGTLQASEALKLLCGIGEPL
                     VGRLLMLDARRTEWTELRVPRQADCPVCSATRAR"
     gene            complement(18842..20275)
                     /locus_tag="PKV30_01430"
     CDS             complement(18842..20275)
                     /locus_tag="PKV30_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRL28914.1"
                     /translation="MSQKTKIAGWIAVGALAGALTTVSLQTVARGTMAPLPLEELQQL
                     AAVFGMIKSDYVEPVDEKKLITDAISGMVSSLDPHSQYFDKKTFKEFREGTTGKFVGV
                     GIEITQEDGLIRVVSPIEGSPADRAGLKPGDLITRIDDTAVKGLSLNDAVKRMRGEPG
                     TKVLLTILRKDENRSFPVTITREEIRTQSVKSKMIEPGYAWLRLSQFQERTVDDFVAK
                     LEDIYKRDPNLKGLVLDLRNDPGGLLDAAVAVSAAFLPEGATVVSTNGQIEESKSIYK
                     ARPEDYMRRRGADPLKRLPAALKNVPMVVLVNEGSASASEIVAGALQDHKRAIVMGSQ
                     TFGKGSVQTVRPLGPDTALKITTARYYTPNGNSIQAKGIVPDIMVDDTAEGSPYAVLR
                     MREADLEKHLTGAQEDKKDPALEKEREAAMKRLEEESRKPAAQRRPPEYGSAQDFPLL
                     QAIAKLKGQPVKVSKTLVERKEEPKTE"
     gene            complement(20401..>20933)
                     /locus_tag="PKV30_01435"
     CDS             complement(20401..>20933)
                     /locus_tag="PKV30_01435"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRL28915.1"
                     /translation="MDRLWLPTVKHWRLNERHYGALQGLNKAETAKKYGDEQVLIWRR
                     SYDTPPPALDAGDARSERADPRYARLNPEDVPLTECLKDTVARVLPFWNESMAPAISA
                     GKRLVVSAHGNSIRALVKYLDHISDDDIVGLNIPNGIPLVYELDAQLKPIRHYYLGDA
                     EAAARAAAAVASQGKG"
BASE COUNT         3353 a         6946 c         7070 g         3564 t
ORIGIN      
        1 cggcacgctg gtggtgctgg tctacacgct gtacggcggc atgtggtcgg tggcgatgac
       61 cgatttcgtg cagatgattg tcatcacgct cggcctgctg gcgattgcgt ggttcgccgc
      121 cggcatggcg ggcggtgccg acaaggtgat cgactacgca gcgcgcgaag gcaagttcag
      181 gttctggccc acggggggcg cgaaagagtg gctgttcttc atcggcgcgg ccatcaccat
      241 gatgctgggc tccatcccac agcaggacgt gttccagcgc gtcatgtcgt caaaagacgt
      301 gcaaaccgcg cagcgcggcc cggtgattgg cggcgtgctg tacatcctgt tcgccttcgt
      361 gcccatgttc gtggtggttg ccgccttgtt ggtgatgccc gaggcggcca gtttgctggc
      421 cgacgacccg caaaaggtgc tgccgcgcct ggtgatggac cacatgccga tggcgctgca
      481 gatcgccttc ttcggcgcgc tgctgtcggc catcatgtcg accgcctcgg ccacgctgct
      541 ggcgccgtcg accacctttg tcgagaacat cctgcgcaac ctgaagccgg gcatgtcgga
      601 cgccgagacg ctcaaggcga tgcgcatcag cgtgctggtg ttcaccgcct gcgtgctggt
      661 gtacgccatc accatgcagg gctcgtcgat ctacgagctg gtggccggcg cctatcaggt
      721 gccgctggtg ggcgccttcg tgccgttggc ctttggcgtg tactggaagc gtgccaccac
      781 gcagggcgca ttgctggcgg tggtgctggg catcggcgta tggctgctgt tcgtggccag
      841 cccgatgctg aaccagggct tcccgcagca actggcgggc gtgctggcgg cggtggcggg
      901 catggtgttc ggttcgctgg cgccgcaatg gctgccagac cacaaggacc acgtgcacca
      961 ctacgagggc agcgcggcgg cttgaggcgg cgtccgccga cgggcgggta ggcgatcggc
     1021 cgtgcgcggc ggaaaatcgg ggcgcgcttt tataatcaaa ggtttttcgc gttcgccctc
     1081 cctagttttt cgtcagcgcc agccatgccc atctacgcct acaaatgcgc cgcctgcggc
     1141 tttgccaagg atatcttgca aaagatgtcc gacgcgccgc tgaccgagtg cccgtcgtgt
     1201 ggccagccgg cattctcgaa gcagctgacg gcgcctggct tccagctcaa gggttcgggc
     1261 tggtacgtga ccgatttccg cgacgggcaa aagaaaagcg gcggcggcca ggagccggcg
     1321 gccgagggcg ccaagcccga aagcggcaag actgaaggcg ccgcccctgc gtcggcgccc
     1381 gcttcggcgc ccgcgcccgc cagcagcccg gcaccggccg cgccgccgcc tgcaggcggc
     1441 ggcggatcgt cttcttcttc ctcgggcagt tgacgcccac cccgcgccat gatgtcccgc
     1501 ctgcggcagt atttcatcac cggcctgctg gtgtggctgc ccatgggcgt cacggtgtgg
     1561 gtgctgctgt ggctggtcgg cattctggac ggcatcttcc tgggcgtgct ggcggcggcc
     1621 gaagcggtga cgccgggcct gacgccgctg gccgatcagt tgcggcgcat tccgggcctg
     1681 ggcgtgatcc tggtggccat cgtcatcttc ggcaccggtg tgttcgtggc caacatgttc
     1741 ggccagtggg cagtgcgcca gtgggacaag ctgatgacgc gcatcccggt cgtgcgcagc
     1801 atttattcca gcgtcaagca ggtgtcggac acgctgtttt caggctccgg ccaggcgttt
     1861 tcgcgcgcgc tgctggtgca gtacccgcgc cagggcgcct ggaccattgc ttttctcacc
     1921 ggccggccgg gcggcgaggt ggcgcggcac ctggacggcg actacctgag cgtctacgtg
     1981 ccgaccacgc ccaacccgac gtccggcttc ttcctgatgg tgccgcgcgc cgacgtgatc
     2041 gagctggcga tgagcgtgga cgaggcgctc aagtacgtca tctcgatggg cgtggtggtg
     2101 ccgccgctgc gcgagccgcg cccgggcgag cccgtcacgg cgcaggtgcc gggcgtgacc
     2161 ggccccaagg cggcggcgcc cgccgctgaa gcacttcctc ctgtcaacca cccgatggtc
     2221 gacccggcca tcgcccgccc cgaactttga aagcagtcct tcccatgtcc atgcgctccc
     2281 actactgcgg tctggtgacc gaagccctga tcggccagac ggtgtccctg tgcggctggg
     2341 tcaaccgccg gcgcgaccac ggcggcgtga tcttcgtcga cctgcgcgac cgcgaaggct
     2401 acgtgcaggt ggtgtgcgac cccgaccggc cggagatgtt caaggtggcc gaagacctgc
     2461 gcaacgagtt ctgcgtgcaa gtcaagggcg tcgtccgcgc acgccccgaa ggcaccacca
     2521 acgacaacct gaaaagcggc aagatcgagg tgctgtgcca cgagctgacg gtgctcaacc
     2581 cttcggtcac gccgccgttc cagctcgacg acgagaacct gtcggaaacc acgcgcctca
     2641 cgcaccgcgt gctcgacctg cgccgcccct acatgcagaa caacctgatg ctgcgctaca
     2701 aggtggcgat ggaagtgcgc aagtttctgg acgagcacgg cttcatcgac atcgagacgc
     2761 ccatgctcac caagagcacg cccgaaggcg cgcgcgacta ccttgtcccg agccgcgtgc
     2821 acgagggcca tttcttcgcg ctgccgcagt cgccgcagct gttcaagcag ttgctgatgg
     2881 tggccggcta cgaccgctac taccagatca ccaagtgctt ccgcgacgaa gacctgcgcg
     2941 ccgaccgcca gcccgagttc acgcagatcg acatcgagac ctcgttcctc ggcgagcagg
     3001 aaatccgcga tctgttccag cgcatgatca ctcacgtctt ccagaagacg atgaacgtgg
     3061 agctgggcga attcccggtc atgccctatg ccgaggcgat gcacaaatac ggctccgaca
     3121 agcccgacct gcgcgtgaag ctcgagttca ccgaactcac agacgtgatg aaggacgtcg
     3181 acttcaaggt cttcagcggc ccggcgacga cgcccggcgg tcgcgtggtc gcgctgcgcg
     3241 tgccgggcgg cagcgagatc agccgcagcg agatcgatgg ctacaccgag ttcgtcaaga
     3301 tctacggcgc caagggtctg gcgtggatca aggtcaacga cgcggcggcc ggccgcgaag
     3361 ggctgcagtc gcccatcgtt aagaacctgc acgacgcggc catcgcggcc attctggagc
     3421 gcaccggtgc gcagaacggc gacatcctgt tctttggcgc cgacaaggcc aaggtcgtca
     3481 acgacgccat cggcgcgctg cgcctgaaga tcggccatgg cgatctggcc aagaagagcg
     3541 gcttgttcga agacagctgg gcgccgctgt gggtggtcga cttcccgatg ttcgagttcg
     3601 acgaggacga ccaccgctgg aacgccgtgc accacccgtt caccgcgccc aaggacgggc
     3661 acgaggacta catggactcg gaccccggca agtgcgtcgc caaggcctac gacatggtgc
     3721 tgaacggttg ggagctgggc ggcggctcgg tgcgcatcca ccgcgccgac gtgcagagca
     3781 aggtgttcga cgcactcaag atttcgcccg aagatgcgca gctcaagttc ggcttcttgc
     3841 tcgacgcgct gcagtacggc gcgccgccgc acggcggcct ggcgtttggc ctcgaccgcc
     3901 tcgtcacgct gatgaccaag gccgagtcga tccgcgacgt catcgccttc cccaagacgc
     3961 agcgtgcgca agacttgctg acgcaggcgc ccagcccggt cgacgagaag caactgcgcg
     4021 aattgcacat ccgcttgcgc aacgcgacgc cggcctgaac gcggccgcgc ctcggcttgg
     4081 atgccgtcct acgccgggcg cgcgggaggt ctgctagcat gggtttctaa tcaggagaat
     4141 cctatgtcat acacctctga tctcgtcgat tcggccaccg aaaccaagga ccagctggtc
     4201 tccaacctgc gccgcgtggt ctccgacgcg gaagacctgc tggcggccac cgccggccag
     4261 accgattcca agatcaccga attgcgtgcc cgcgcaaagg aaaacctgtc gatggcgcgc
     4321 gagaagctgg ccgatgccga cgccgtggtg cgcgcccgcg cccgccaggc cgcagcggtg
     4381 accgacgagt acgtgcacga caacccatgg tcgtcgattg gcgccgccgc ggcgctgggc
     4441 attttgatcg gcgtgctgct cggccgtcgc tgattgtttc gtttcctctc gccatgagcc
     4501 tgccttcatc cgacgccagc accgccgcgc gcgtgcgcgg cctgctggcc gatgtgatcg
     4561 agctggctca ggtgcgcttc gagctgttca cggtcgaagc gcgtgaagaa ctggcgcgtc
     4621 tggcaggcat ggcggtcatg ggcgcgctgg cggtggtgtt cgtcagcttc gggctgatct
     4681 ttctggccat cttcctgacg gtgctgctgt gggacacgca ccgcgtgctg gcgctcggca
     4741 tcttcacctc gctgtttctg ggcggcggcc tggtgctggc gctgctggcg tggaacaagg
     4801 ccaagcaggg ttttcgcatg tttggcgcca cgcgctccga gttgcagcgc gatcaggaca
     4861 ggcttcgcac gtcatgaagg acgatcccag cctcaacctg gccacgcggc gcgagcaatt
     4921 gcgcctgcgc tcggcgcagt tgcgcgagca gattgccgtg cgcagccagg tgttccagcc
     4981 cgcgtttcgg gccaccgacc gcgtacgcgg cggcgtgcgc agtgtgcgcg gcgtgcggga
     5041 caaccgggcg ctgctcatgc tgggcggcgc ggctttggcg ggcgccttgc tggtgcgccc
     5101 gcgcgcggcg ctgcaactgg gcatccgcgc ctggtcgggc tggcaggtgt accgccgcat
     5161 gcggccgctg gtcaacagcg tgttgcgcca gctgggttga ttgtcggcgg cggcgcgtcc
     5221 gcgaagtgtg gccacgcacg cgaagtcaaa cagccatgct caagcccttc aagatccccg
     5281 aatcggtgct ggtcgtggtg cacacggcgg cgcttgatgt gctgctgatc cggcgtgccg
     5341 acgctggcgc ggatgaattc tggcaatcgg ttaccggcag caaggacgcg ctggacgaac
     5401 ccctggcgca caccgcggcg cgcgaagtgg gcgaagaaac cggcatcgac gcgcttggcc
     5461 ccgattgcgc gctgaccgac tggcgcatcg agaacctgta cgacatctac ccgcgctggc
     5521 aggcgcgcta cgcgcccggc gtcacgcgca accgcgaaca cgtgtttggc ctctgcgtgc
     5581 cacgcgatgt gccggtgctg ctgaacccgc gcgagcattc cgacttcgtc tggctgccgt
     5641 accgcgaggc ggccgaccgc tgcttttcgc cctccaacgc cgaggcgtgc ctgctgctgc
     5701 cgcgtttttc caaggtgacc gcatgacggc agattcgagt tttggcgagc cgcacgacat
     5761 cctgcgggtg gccacctaca acatccacaa gggcgtgcaa ggtgtcgggc ctacgcgccg
     5821 gctggagatt cacaacctgg cgctggccat cgagacgttg gatgccgaca tcgtctgcct
     5881 gcaggaagtg cggcaaggca atcgcaagga ggcgagctat ttcaccggct ggcccgagca
     5941 gccgcaggcc gagtttctgg cgcctgaagg gtatgaggcg gtctaccgct ccaacgccgt
     6001 gaccaagcac ggcgagcacg gcaacgcgct gctgtcgcgc tggccggtca tcgagtgccg
     6061 gcacgaagac atgtcggacc accgcttcga gcagcgcggc ctgctgcacg ccaagctgct
     6121 ggtggcgggc gtgacggtgc acgccatcgt ggtgcatttc gggctgattc cgtccagccg
     6181 cgtgcgccag accgcccacc tcaaggccta catcgcgcgc gagattccgc tcgacgagcc
     6241 ggtggtggtg gcgggcgact tcaacgactg gggcacgcgc gtgcgccggc tgttgcacgt
     6301 ggattcgctg cacgccttcg aggggccgcg cacgctgacc tacccgtcgc gctatcccgt
     6361 cgcgcagctc gaccacatct acgcccgcgg cctgcgcccc ttgagcctgg cggcgccgcg
     6421 cgggcgcgtg tggagccgca tgtccgacca cttgccgctg atcgccgaat tcaatctgcc
     6481 gcagtagaag gctacaaaaa gtagagctgc ttgcgcttgc ctggcaagcg ctgcacggca
     6541 tttcgatgac gaaaaaactt tcgctgcgcg ccatgacgaa atgaccggac gctccgacat
     6601 gccgcgcagc gccgtcatcg atggcgtcag ccgagatcat gcgccgtcag gcgcgtcatc
     6661 aaaattcata gctgctcgcg cttgtccaac aagcgccagc ggctgattca acgaaaaacc
     6721 ctttgctgct accatcggtg cgctcatgaa agcttctccc gatctccccg cggcggccga
     6781 cgcctccgac gaaggcacgc ctgtctcggt caagattcgc gagcgcctgc aggccgcgcg
     6841 ccgccgcttc aacgccaacg acaacatcgc cgatttcatc gaacccggcg agctggaaaa
     6901 actgctcgac gaagtcaccg ccaagatgga aggcgtgctc gacagcatgg tgatcgacac
     6961 cgggcgcgac cacaacaccg ccaacaccgc gcgccgcgtg gccaagatgt acgtcaacga
     7021 ggtgttcaag ggccgctacg tcaagccacc cgagatcacc gagtttccca acgccgagcg
     7081 cctgaacgag ttgatgatcg tcggccccat caccgtgcgc tcggcctgca gccaccactt
     7141 ctgtccggtc attggcaagc tgtggatcgg cgtgatgccc aacgaacgca ccaacgtcat
     7201 cggcctttca aagtacgcgc gcctggccga gtggatcatg ggccgcccgc agattcagga
     7261 agaagccgtg gtgcagctgg ccgacttgat ccagcaaaag accaagcccg acggcctggc
     7321 gctggtgatg gaggccacgc acttctgcat ggcctggcgc ggcgtgaagg acatggacag
     7381 caagatgatc aactcggtca tgcgcggggt gttcctgaag gacatgaacc tgcggcgcga
     7441 attcctttcc ctcattcccc agaagactta acctaggaac ggcaaatgac cgcttccata
     7501 cctccatcaa tgcttcttgt gattaacgtt cttgacttcg ctgacgttcc cgttttgggt
     7561 gagcgcgcta cgtgcccgat cgcaccgcgc tggccgcgcc atgcagcgtt gctcgtcctt
     7621 gcaggcagtg gcctgccgcg tcctcgcgcc ttgcctgaca cgcccatcat ggcacgctcg
     7681 ggcccgttca tctgccgttt ctaggttaag catgctcgtc cgtctgcttt acgccagccg
     7741 cgccgtcgac accacgccgg ccgccatcga agccatctgc gccagcgcgc gccagcacaa
     7801 cgccgagcag ggcgtcacgg gcgtgctggt ctatggtggc ggcatcttca tgcaagccat
     7861 cgagggcggc cgccagacga tcagcgacct gtacggcgtg atccagaggg acggtcgcca
     7921 ccaggatgtg gtgctgctgc attacgaaga gatcgccgag cgccgcttcg gcggctggac
     7981 catggggctg gtcaacacca gccgcatcaa ccccagcacc ttgctcaagt acagcgaacg
     8041 cgccgagctg aacccttatg ctgtctctgg ccgcgtgtcg atggcgctga tcgacgagct
     8101 gatggccacg gcctcgatcg tcgggcacac ctgagccgcg cggcgcgcgc agcggcccat
     8161 caaatcatcg cggctgccaa gtcggccgat tcgtcgccgg tcgagcgcag cgcgctcagc
     8221 gcctcggcgt cggtgcggtg caagcgcagc ccggcgcgcg cgttcagcag gccggcgcgc
     8281 gccagcggct gctccaccgg caatttcagg cccaccagat ggaaggcgat gtgctgcccc
     8341 atcaggtgcg cggccagctt gccaaaggtt tccacgcccg tcgcgtcaat gcggttaaca
     8401 ggttgcatga acagcgccag gtgccgcgtg tcggggttga gggtcagcgc atcctgcacc
     8461 gcgcgctcga aggcggcggc ggtggcaaag tcgagcgcgg cgtccatgcg cagcgccagc
     8521 acgcgcggcg cgatgggcgg caggcgccac agcgtgcggc tgcgcaggct gccgtccggg
     8581 tgctcgccca cttccaggat gcgtgggtgc aggtgctggt acagaaagtg gctgagcgac
     8641 atcagcacgc ccgccagcac gccccagtac agcgccggtg ccgccagcag cgtgacgaca
     8701 aaggtgaccg cggcgatcac cgcctccagc cgcgacacct gccacaaccg gccaaactcg
     8761 cgcggcttga gcaacccata gacggtggcc accacgatcg ccgccagcac cgcctgcggc
     8821 acgtgccgca aaaccggtgt gaagaccaac aacgccagcc cgaccacgac ggccgagaag
     8881 atgctggccc agcccgtctt ggcgcccgcg tacaggttga gcgccgagcg cgagaaggac
     8941 gagctggtcg gaaaggcgcc cgacaggcca gaggccagct tggccagccc ttggccgatc
     9001 aggtcctggt tctggtccca gcgcgcacca cgcgccgcgc tgtccacctt ggcgctggaa
     9061 gcggtttcga gaaagctcac cagcgtgatg atgagcgtcg gcatcaccag ctgccccagc
     9121 gtcgcccagc ccggccagtg cggcacgaac aagcccggca ggccctgcgg cagcggcccg
     9181 accacggcgc cgccttgcgc agcgtagggc gtgaaggcgc tcacggccgc cgccaccagc
     9241 accacgacca gcacgcccgg aaagctgggc ttgagccgcc gcgccaccac cagcagcgcc
     9301 agcgcgccga tgccgaatgc agcggacgcc caatgcgcgc ccgtcaaacg cgctgcccaa
     9361 tcgccgtctt tcggcagccc gagcagcgcc ggcaactgcg aaaaaatgat gagcaccgcc
     9421 gcgccttggg tgaaggccat cagcaccggc gccgtcacca ggttgagcag ccagccggcc
     9481 tgtaccgcgc ccagcccgat ctgcagcgcg ccggtcagca gcgccagcca caccgccagg
     9541 ttgacccatt ccgcgctgcc aggcgccgcc agcccggtga gcgaagccgc caccagcagc
     9601 gacgacaagg ccgtcggccc caccgacagg cgcggcgaag ccgagaacag cgccgccacc
     9661 agcgccggca gcatcgaggc gtagatgccc gtcaccaccg gcatgccggc cagcgcggcg
     9721 taggccacgc cttgcggaat caccatcagc cccaccgtga ggcccgccag cgcctcgccg
     9781 cgcagcagcg cgccgctggg gcgaggccag ttcagaaagg gcaggcggcg ggcaagggcg
     9841 cggtgcatcg ggggcgatgg tacggcaggc ggggcatcga accaggcagc gtcagcccac
     9901 caggccattg aggcgttaga cgacgacaat gatgaaaacg ggcgccgttg agcgcgcccc
     9961 cgaaccggag ccccccatga accgccttgc cctcgttgcg ctgttcgcgc tgctgcctgc
    10021 tgtggtcggc gcgcaggcca tccgctgcaa agacccggcc accggcaaga ttctgtacac
    10081 cgaccagccc tgcaagggtg gcgagttggt ggtgccccag cgcagcgagg ccgacatcgc
    10141 gcgcgacgcc gccaacgccg ctgcagcgcg tgagcgcgag tacgaccgcg ccctgcagca
    10201 gcgcgaggaa cgcgccctgc agcgcgaaca actgcgcgcg cagcaggctg ccctgccgcc
    10261 gcccgcgccg ccggccgaaa ccgacgcctg ccgcgccgca cgcgccgaag cggcgttccg
    10321 cgccggcagt ttttcggcca gcgcggaaga aatccgcacc gcgcgctaca acgccgccct
    10381 ggcctgcggc cagcagccgc cggccgacat cgtggtggtg cagccgccgg agccgctgta
    10441 cccggtgcgc cggccgccgc gctttgacga cccgcgccgc ccccatcgcc cgggcgatgg
    10501 cttcggcatg ccgcggccag cgccgcgccc gcagtacgag ccgggcaccg aacccgttcc
    10561 ggtgctggtg cggccgccgt cggcacgctg atcccacgcg cctcagcgct gcggcatgtc
    10621 cttggcgccg tagcccaggc tgacggtggc gtcgggcgga atggcgggca tgctggcgtt
    10681 ccacgcctcc cacgcgtcgc gcagggccgc cagccgttcg gattcgcggg cggccaggtt
    10741 ggcgcgctcg cgctcgtctt gggacaagtt gaacaggtaa tcgtggccat caacgcgcag
    10801 gtacttccag tcgccgtcgc ggtgcgcgcg ctggccgcgg tggttcatgc gccagaacag
    10861 cgggcgagca aaacgctgcg ccacatcgcc cagcaccggc agcagcgaga cgccgtccag
    10921 cgggtagtcg gggtgcgcgg ccacgccagc ggcgtcgagc atcgtcgccg accagtccat
    10981 cgtcatgcag gcttgcgggc tgacgccgcc cgccgcgatg cgcgccggcc agtgcgcgat
    11041 ccacggcacg cggatgccgc cttcggtcaa atccatcttg ccgccgacca gcggccagtt
    11101 gtctgaaaag cgctcgccgc cgttgtcgct ggtgaagacg atcagggtgt tgtccagcag
    11161 cccttcagcg cgcaaggtct cgaccagctg gccgatgccc tcgtccatgt ggtgaatcat
    11221 gcggcggtag gtgtggatgt tgccgccgtg caggtggaac aggttgtcct tgagctcctc
    11281 ggccagcgcg gcgtcgcccc gcgtctccca cggccagtgc ggcgcggtgt agtgcacgct
    11341 caggaaaaac ggcgtgccgc cccgcgcgcc aggcgccatg cgctgcagcc agtcgaccga
    11401 gcgctcggtg atcaggtcgg tcaggtaacc ctcttcgtgg tgctcggcat cgcccaggta
    11461 cagatcgtgc gtgccgttgt tgctgcagtg ggtgaagtag tcgacaccgc ccgagatggg
    11521 gccgaagaat tcctcatagc ccgacatcaa cggcccgaac accggcgggt agcccaggtg
    11581 ccacttgccc atcagcgcgg tgcggtagcc ggcgcccttc agcagcgagg gcagcgtggg
    11641 gtgctcgggc ggcaggccga gcgtggtgct gcccttgctt ttgctgttga tcggctcctc
    11701 ggccgcgccg cgcaggcggt actggtagcg cgcggtcatc aggccaaagc gggtgggcga
    11761 acacaccggc gagttgctgt agccctgcgt gaacttcagg cccgcagcgg ccaactgatc
    11821 gagcaccggc gaaacggggc cgaaggcggc atcgcgcccg ccgtagcagc ccaggtcggc
    11881 aaaacccagg tcgtcggcga cgatgaagat gatgttgggg cggcttgttt tttgagtcat
    11941 ttaggcctct ggcgcttgtc tggcaagcgc gagcagctat caaaaatata gaaacgtcac
    12001 tccaccttca tgcccgtggc ctgtatcacg cgcccgtagc gcgtggacag atcccccagc
    12061 cgcttggctg cggcggcgcc gctcaggccc tcgtagaaca aatccagatt ggccagccgt
    12121 tgcttcacct gcggctcggc cagcgcgtcg gcaaaggctt tttgcagcgt ctgcaccacc
    12181 gcgtccggcg tggcggcggg caccatggcg atgtagagca cttcctgctc cagacccttg
    12241 tggccggctt cggccaccgt gggcacttcg ggcgccaggg tgctgcgctg gcggctggtg
    12301 acggccagcg ccgtcacttt gccggctttc acgtgcggca gcaggcccgg cgtggccagc
    12361 acgccgccat tgacttcgcc gctcagcacg gcggtgacgg ccggcgtgtt gcccttgtac
    12421 ggtacgtgcg tgatctgcat gccggtggct tcatgaaaca cctcggccgc cagatggccc
    12481 gggctgccag aaccggccga gctgaaggtc acgccctggg ccttgccggc attcaccaga
    12541 tcggccagcg tcttgaagcc ggtcgacggg tgcgcgccga ccagcaggcc cgacgaggcc
    12601 atcaccatca gcggcttgaa gtcgccgggc ttgaacggca accctttgta gatgtgcggg
    12661 ttgacggtga agatggtgtc gatgccgatc agcaccgtgt ggccgtccgg cgcgctgcgc
    12721 gccacctgcg cggtgccgat gttgccggcc gcgccgggct tgttctcgac cacgactggc
    12781 tgcttgaagc gctgctgcag cacctcggcg accgagcgcg cgatcatgtc cgacgggccg
    12841 ccggccggaa agctgttgac gaaggtgatc ggcttggtgg gaaagccttg cgcgctggcc
    12901 gcgctcgcca ccaggctgaa cgtggcggcg gacagcgtgc gccgggcgct ggtgaagaag
    12961 ggcgttgcgg tcatgatttt tgaataaaaa gtggcgccag cgcttgtctg gaaagcgcga
    13021 gcagctcatt tttcaatagc gaataaactc agggctgata gcccgatttg cgcaccaccg
    13081 gcgcccactg cgcgcggtaa gccttcagca tggcggcggt ctgcgccgcc gtgctggcca
    13141 ccggggtcat cttggcgtcg gcaaagcggc gccgcgtgtc ggggtcgttc atcacgtcgc
    13201 ggatggcggc cgacaggcgc tgcacctgtt cgcgcggcat ggcggcgggc gcgaagaacg
    13261 cgttccagcc ggtggcgacc aggtcgagcc cggattccct gtacgtcgga atgtccttgg
    13321 caaagctcga ccgctgcgcg cccgagctgg ccaggatgcg cagcttgccg ccttcgtgct
    13381 gcggcagcag ggtgtccagc gtgtcgaagg cgaccggcac ctggccgccg atcaggtcgg
    13441 tgatgagcgg gccggagccg cggtagccca cgacctgccc tttcacgtcg gccttctcgg
    13501 cggtcatcag cgcaaagaag tgcggcaggc tgcccgtggc gggcacgccg aagttggctt
    13561 gcgccgggtt ggcgcgcagc cacgccagca ggtgcgacaa ctgcttgacc ggcaccgcct
    13621 gcgccaccgc caggccaaac tcgtaatcgc tgacctgcga cacgggctgg aagtccttgt
    13681 cggcgtcgta gccgttgtcc ttgaacacca gcggcgccac caccatgatg gccgggttgg
    13741 ccagcatcag cacgttctgg ctggccggcg cgtttttcag ctgctgcgcc gccagccggc
    13801 cgccggcgcc ggtgcggttt tcgaccacca ccgtctggcc gagcttgggg cccagcttgt
    13861 cggccaccag ccgcgccgcg cggtcgctgg agccgccggg ggcgtagggc acgacgaggc
    13921 gcagcgtcgt atcggcttgc gcgtgggcac ggctggtcag ccagcccagg ctggcggcgc
    13981 tggcggcctg aatcaaatgt cggcggtgca tggggaatct cctcgtctgc gtggatcggg
    14041 actctggcac tgtaggtggc gcggcggcaa ttgacaaaat caactgttct caggacttac
    14101 cccgatggcg accaaccgtt tgaccgagcc caagacgctg gacgacatgc tgctgtacgt
    14161 gctgtggcaa ctgcaggcgg cggcgcgcgg gccggtggtg cgcgtgtgcg aggccgagtt
    14221 cggtatcacg cggcgcgaat ggcgcatgct ggcgcaactg gggcaggggg aagggctggc
    14281 gtcgtcggca ctggccgagc gcgcggggct ggaccgggcg cagacctcgc gcgccgtggg
    14341 cggactggtg ggcaagcggc tgctggcccg cacgccgcgc ccgggggatc ggcgcgaggt
    14401 gctgctgcac ctgacggacg aagggcgggc ggtttacaac gcgctgctgc cgcgcatcgc
    14461 ggccatcaac cgcgatttgc tctcggtgct ggcgccggcg gaagtcgagg tgctggacgg
    14521 cttgctgcga cgcctgaatg cgcaagccct gcgcgtcggc gcggtgggtg acgatgtggt
    14581 gccgaaattt gaatgaaatc ggcctctggc gcacgtccaa tcagcgcaaa cagctatgaa
    14641 aaacatagtt caaatccgtc ggaaagcgcc aagaaaaaac cctgccagcg agaccggcag
    14701 ggttgttctc agcccagctg cagctggctt ggcaggcgat cagccgcggc ggcggcccat
    14761 cagcagcgcc agcgcggcac ccgcggcggc ggcaatcgcc atcgacttca gcggctgctc
    14821 agcgacgtag gcgctggtct tgtcggtgta ttcgttcagc ttctcgcgcg tctgggcaga
    14881 cgtttcggcg gctgcggcct tgccgcgcgc ggccatgtcg tgcacgcgcg aggagatggc
    14941 gtcgatggcg ggcttgacgt cttcgcgcag gctgcgcacc ttggcgtcgg ccttgtcgag
    15001 cgcctcgttg gcgtacaggc gggccgattc gaccgagcgc tcggccttgt cggccatctt
    15061 gtcggtggcc acttcggcct tgtcggcgat tttctcgacg gtggaatacg agttcatgtc
    15121 agaaggtcct ttctgggcgc gtgcgccggt gggtgatgca agggtggagg cagcgcttgc
    15181 tcaggcacgc agtccatgag ccgaaagcgt ctgcagagca taggcgatgc ggggtggcca
    15241 gcgcatcaga tggccgcgtc gtgcgctgtc gtctgcgggg acggcgcctt gtaggaatcg
    15301 gacgacatgg cgcccaccga ctcggccagc gcgctttgcg gcttggacac cgcgcgcgga
    15361 atggtgccgg tgatgcgcga gccgccgccg cccgcgcgcg aattgatgtc cagccgcccg
    15421 ccactggcct cgacccggtg gcgcatgccg accaggccgt gcgaggcctg cggcagccgc
    15481 gtggcgtcga agccgacgcc gttgtcctgc acggtgactt cggcgtggta ggtgtagttc
    15541 ttcacccgca cgatcacctt ggtggcattg gcgtacttgc cgatgttggt cagcgcctct
    15601 tgcaccatgc ggtaaatggt cagttcgctg acttcgtcga gttcgaccgg ttcgagaaat
    15661 gtctctattt cgacgcccga gcggtccgca aactcgcgcg tcaggatctc gagcgacgcc
    15721 accagcccca ggttggacag cgaggagggg cgcaggtctt cgatgatgcg gcgcttgagc
    15781 gcaatgccct ggttgagcgt ctcggtcagg tgtttcaggc gctcggccag ttcgtcagcg
    15841 tctttgggca gcttggactt gatacgtgcc acgttcagct tggccgctgt gagcatcgcg
    15901 cccaattcgt cgtgcaactc gcgcgccaga tgggcgcgtt cgtcttccac cgcctgctgc
    15961 agataagtgg ccagctcggt caggcgggca gtgcgctcgc gcacctgcga ttcaagcgcg
    16021 tcgcgctcgg cttcaagcaa ctgtttttgg cgcgcatcgg tctcgcgcag cgtgcgcgtc
    16081 tggttgacgt acagaaagaa agcaaagaac gctgccagaa cgccggcagc cagcccgaag
    16141 cgcgacacgt tcagcagccg atacagctcg gcgcggttgg cggcaaccac cgcgtctgct
    16201 tgcgccagca gcgcatcgcc ctgcaactga aacgcgcgca tctgctccag ccccacgttc
    16261 gaggtcatga cgaaattcac cacctcggcc cgctcttcct ggcgcagtcg caccgtcagt
    16321 gccatctcgc cgatcttttg cgacagcagg tggcggaagg cctgcatcgc ttcgctctcg
    16381 ttcatggcgc gcggcacgtt ggcgcccagg cgcttgagca gcacgtcgat gtccttcaag
    16441 gccgcgtcgt agggcgccag gtaggcgcga tcgccggtga ggatgaaacc gcgctggccc
    16501 gactcggcct cgaccagccg gcgcaccacg gtgttgatgg tcgagcgcgt ggcgtggccg
    16561 tgctggatct gcaccagcgt ctgcgtggtg cggtggtacg tgtcctcgtt cagccacatg
    16621 atggcgatgg cgcacagggc ggcgatgacc agcgtgatgc gcagcgacag cggcttgcgg
    16681 gcgcgctggg cgtcgtcttg agtcggggcg cttggatcga gcatgtctcg ggcaactaga
    16741 atcacggtct tgcggctgca tggcgcggcc tggataacct caaagcggaa ggcgcaccgt
    16801 gatcagaatc ggtattgtgg atgaccacgc cattgtgcgt tcgggactga agcagttttt
    16861 ttcggagcat gtggatctgc gggtggtcgg cgaggcggcc agcggccgcg aagcgatcga
    16921 cctggtgcgc accaccgaaa tggacgtgct ggtgatggac ttgtcgatgc ccggccaaag
    16981 cggcatcgac gcgctggcca tgatccgcgc caaggcgccg gacgtcggca tcctgatcct
    17041 gtcgggctac cccgaagaac actacgcgat gaacctgatc cgccagggcg cgagcggcta
    17101 cctgaacaag gagtgcgacc cggccgagat cgtggaagcc attcgcacca tcgcgctggg
    17161 caagcgctac atcacgccga cggtggcgga attgctggcg cagcagctca accgcaagaa
    17221 cgacgcgctg ccgcacgagc agctgtcgga gcgtgaattc caggtgttcc tcaagctggc
    17281 caaggggcag actgcgggcg acatcgccga ttcgctgtcg ttgtcggtca agaccgtcag
    17341 cacctaccgc acgcgcctga tggagaagat gagcctcagc tcgaacagcg acctgaccta
    17401 ttacgcgctg aagaacaagc tgatcgactg aagcgccgat ccggcacggc cggagccaag
    17461 cataaaagcc gccttgggcg gcttttttat ggagggcgtc gcgggcgccg caagcgcaca
    17521 ggttcaggcg ttgcccttca tctcaagaca gaaatcgatc aaggcctcga tctcgttgga
    17581 tttgtcaaac acggcatcga cgcccagttg atggcagcgc acgcgaatgt cgggcgtggc
    17641 gtagttggtc agcaccacca ctttctgctc ggggttgcgt tcgcggcagg ctttcagcac
    17701 gccaaggccg ctgccttgct tgaggaacag gtcgacgatg gccaggtccc agttggcgct
    17761 gtggttggtc agccattcgc tgccctgttg ctccgtctcg gcatagccga tgggtgcgac
    17821 gccacccagt tcttcaagcg tggcaatcag gttctcgcgg atggtcgggt tgtcttcaac
    17881 gaaataggct cgcactgggc tggggccggg catggtggca atgtgaaacg aaatggcctt
    17941 gaggggtcgc ttgtcttggg ctcctgacca ccgtcaagga aacatggcgt gagtttaggg
    18001 gtgcgaacgc gcacgcactg acagcgtgca caccaccgcc gagtaggata aaacctacct
    18061 ggcccgtgtg gcgctgcaaa ccgggcagtc tgcttgccga ggcacccgca gctcggtcca
    18121 ctcggtgcgc cgggcgtcga gcatcagcag ccggcccacc agcggctcgc cgatgccgca
    18181 cagcagcttg agcgcttcgc tggcctgcag cgtgccgatg atgccgacca gcggcgcgaa
    18241 cacgcccatg gtcgcgcagc gcgcttcttc caccgcttgc gtcggcggga acacgcaggc
    18301 atagcagggc gaggcggcgc ggcgcgggtc gtagaccgag atctgcccat cgaagccgat
    18361 cgccgcgccc gagaccagcg ggcggccggt cgccacgcac gcgcggttga gcgcctggcg
    18421 cgtggcgaag ttgtcggtgc agtcgagcac gatgtcggcg gcggccgcca gttcggtcag
    18481 ctgcgcttcg tcggcgcgct gcgccaccgt gcgcacgcgc acgccggggt tgagcgcgcc
    18541 aatggcggct tcggccgacg ccactttggg ctggccgacg cgcgcctcgg tgtgcatgat
    18601 ctggcgttgc aggttggtga ggtcgaccgt gtcgtcgtcg accatcgtga tttgcccgac
    18661 gccggcgctg gccaggtaca gcgccaccgg cgaacccagt ccgccggcgc cgacgatcaa
    18721 cgcgtgcgaa tcgagcagcc gctgctgccc ctcaatcccg atgtcgtcga gcagcaggtg
    18781 gcgcgaatag cggagcagtt gccggtcgtc catctgcggc acctgcgctc agcgcgcagg
    18841 cttattccgt tttcggctct tccttgcgct cgaccaaggt cttgctcacc ttgaccggct
    18901 ggcccttgag cttggcgatg gcctgcagca gcgggaagtc ctgcgcgctg ccgtactcgg
    18961 gcgggcggcg ttgggcggcg ggcttgcgcg actcttcttc cagccgcttc atggccgctt
    19021 cacgctcttt ctccagcgcc ggatctttct tgtcttcctg cgcgccggtc aggtgcttct
    19081 cgagatcggc ctcgcgcatg cgcagcacgg cgtaggggct gccctcagcg gtgtcgtcca
    19141 ccatgatgtc gggcacgatg cccttggcct ggatcgagtt gccgttgggc gtgtagtagc
    19201 gcgcggtggt gatcttgagc gccgtgtcgg ggccgagcgg gcgcaccgtt tgcaccgagc
    19261 ccttgccgaa ggtctggctg cccatgacga tggcgcgctt gtggtcctgc agcgcgccgg
    19321 ccacgatttc gctggccgag gccgagcctt cattgaccag caccaccatc ggcacgtttt
    19381 tcagcgccgc cggcagccgc ttgagcgggt cggcgccgcg gcggcgcatg tagtcttcag
    19441 ggcgcgcctt gtagatcgac ttgctttcct cgatctggcc gttggttgac accacggttg
    19501 cgccttcagg caggaaagcc gccgacaccg ccacggctgc atccagcagg ccgcccgggt
    19561 cgttgcgcag atcgagcacc aggcctttca ggttggggtc gcgcttgtag atgtcttcca
    19621 gcttggcgac gaagtcgtcg accgtgcgct cctggaactg cgacaggcgc agccacgcgt
    19681 agcctggctc gatcattttc gacttgaccg attgcgtgcg aatctcttcg cgcgtgatgg
    19741 tgacggggaa gctgcggttc tcgtccttgc gcaggatggt gagcagcacc ttggtgcccg
    19801 gctcgccgcg catgcgcttg acggcatcgt tcagcgacag gcccttgacg gccgtgtcgt
    19861 cgatgcgggt gatcaagtcg cccggcttca ggcctgcgcg gtcggccggc gagccctcga
    19921 tgggcgacac cacgcggatc aggccgtctt cctgcgtgat ctcgatgccg acgccgacga
    19981 acttgccggt cgtgccttcg cggaattcct tgaaggtctt cttgtcgaaa tactgcgagt
    20041 gcgggtcaag gctcgacacc atgcccgaaa tggcgtcggt gatgagcttt ttctcgtcca
    20101 ccggctccac gtagtccgac ttgatcatgc cgaacacggc ggccagttgc tgcagctctt
    20161 cgagcggcag cggcgccatg gtgccgcgcg ccacggtttg cagcgatacg gtggtcagcg
    20221 cgccggccag cgcacccacg gcgatccatc cggcgatctt ggttttctga ctcatgtctg
    20281 gcgatttcct atggggttcg ggcgcaatat atacgttgcg actgctttcg gctcgaatgc
    20341 ggcagcgaag ttccgtgacg gacgatggcg tttacgtggg attcacattt cgtgccggcc
    20401 tcagcccttg ccctggctgg cgacggccgc cgcggcccgc gctgccgcct cggcgtcgcc
    20461 caggtagtag tggcggatcg gcttgagctg cgcgtccaac tcgtacacca gcgggatgcc
    20521 gttggggatg ttcaggccca cgatgtcgtc gtcggagatg tggtcgaggt acttgaccag
    20581 cgcgcggatc gagttgccgt gcgccgacac caccagccgc ttgccggccg agatggcggg
    20641 cgccatcgat tcgttccaga acggcagcac gcgcgccacg gtgtccttca ggcattcggt
    20701 gagcggcacg tcttcggggt tcagcctggc gtagcgcggg tcggcgcgct cgctgcgtgc
    20761 gtcgcccgca tccagcgcgg gcggcggcgt gtcgtagctg cggcgccaga tcagcacctg
    20821 ttcgtcgccg tatttcttcg cggtttcggc cttgttcaag ccctgcagcg cgccgtaatg
    20881 gcgctcgttc aggcgccagt gcttgacggt gggcagccac aggcggtcca tct
//
LOCUS       DAOMVO010000012        19482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_505_length_19482_cov_13.932980, whole genome shotgun sequence.
ACCESSION   DAOMVO010000012 DAOMVO010000000
VERSION     DAOMVO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 19482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19482
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_505_length_19482_cov_13.932980"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="fahA"
                     /locus_tag="PKV30_01440"
     CDS             <1..911
                     /gene="fahA"
                     /locus_tag="PKV30_01440"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRL28916.1"
                     /translation="YTDFYTSVHHATNIGKQFRPDNPLLPNYKWVPIGYHGRSSSIGI
                     SGQSFKRPKGQVKPPDADAPVLKPSARMDIELEMGVFVGQANELGEPVPMTEAEDHIF
                     GLVLFNDWSARDIQPWEYQPLGPFLSKNFASTISPWIVTMEALAPFRAPFSHPAEDPQ
                     PLPYLSSADNSAQGGLDIHLEALIETPKMRDAKAAPARFTQTNYRHAYWTAAQMVAHH
                     TVNGCNLQPGDLLGTGTLSGPTLDQACALIELTTGGKNPIQLPNGESRTWLEDGDTVI
                     LRGWCEKPGAARIGFGECVGTVLPAT"
     gene            979..1236
                     /locus_tag="PKV30_01445"
     CDS             979..1236
                     /locus_tag="PKV30_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRL28917.1"
                     /translation="MHAWPVQDAKARFSEFLDACLSDGPQLVTRRGAEAAVLVPMHEW
                     RRLQRAARPSLKELLLTDEARADLVLPARGKARRRPVAVPD"
     gene            1236..1643
                     /locus_tag="PKV30_01450"
     CDS             1236..1643
                     /locus_tag="PKV30_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL28918.1"
                     /translation="MYLLDTNVVSELRRPRPHGAVLAWLQAVDDAQLFISAVTLGEIQ
                     AGIELTREQDTDKASEIEAWLDQVAAAYNVLPLDAAAFREWARLMHRRSDTLYEDAMI
                     AATARVHGLTVVSRNVADFKALGMEALNPFTAR"
     gene            1665..2879
                     /gene="dinB"
                     /locus_tag="PKV30_01455"
     CDS             1665..2879
                     /gene="dinB"
                     /locus_tag="PKV30_01455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRL28919.1"
                     /translation="MRRIAHLDMDAFYASVEWLRYPQLKGLPMVIGGGRRKEDDLIGR
                     LNAERPERHWTSADLADIPVDFFPLLRDYVGRGVITTATYAARPFGIGSAMGLMKAAK
                     LCPQAILLPVDFDQYRKYSRAFKQVIRDIAPVMEDRGVDEVYIDFTDVPGGQREGGRV
                     LARLIQKSIADTTGLTCSIGVAPNKLIAKMASEFNKPNGISIVFEHDLERLIWPLPCR
                     KVNGVGPKADAKLQGHGIHTIGELAARERAWLIQHFGKAYGAWLHDMAWGRDERPVVT
                     ESEPVSMSRETTFERDLHAVRDKAALSAIFTTLCQQVAGDLQRKGYVGKTIGIKLRFD
                     DFKIATRDQTVAEHTADAQRIRQVAGQCLKRVPLDRRLRLLGVRVGSLARQSDLARGG
                     DSEPNLALPLFQ"
     gene            3073..3918
                     /locus_tag="PKV30_01460"
     CDS             3073..3918
                     /locus_tag="PKV30_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRL28920.1"
                     /translation="MNTAALTCFQLTVTDHIAHLVLSRPEAMNTMGPTFWRELDQVLT
                     RLNQSGEARVLVVSSTGKHFSAGMALDVFGGAISMDDATPEGRAAIADLLGGLQATFT
                     RLETLRIPVIAAIQGGCVGGAVDLVTAMCVRYATHDAFFVIQEINIGMVADVGTLQRL
                     PKLVPLAVVKELAYTGRRLPAAKARDYGLVNDVFDTPEACLAAAMQCAKEIVAKPPVA
                     IWGTKQVIHYARDHSVDDALRQMGWVQGAIWSNAHVREAVTAMKTQREAQFPSLAELK
                     GFGAE"
     gene            complement(4107..4373)
                     /locus_tag="PKV30_01465"
     CDS             complement(4107..4373)
                     /locus_tag="PKV30_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HRL28921.1"
                     /translation="MNALDLLGLVATCFTTSSFVPQVWRTWKTRDVSGISLPTYVIIT
                     IGLALWLVYGWLRGDMPLMVANAVMVVLTGAITVMKLRFEQRAA"
     gene            4458..5459
                     /locus_tag="PKV30_01470"
     CDS             4458..5459
                     /locus_tag="PKV30_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRL28922.1"
                     /translation="MTTPPLPRAFVLGNFVQACCWHVARLPQAGETLEAHHLQIEPGG
                     KGLNVAVCLQRLGVAVDTLIGCGDDAAGDALLALLAREGLATTHVHRLAGPSGWGAGL
                     IGADGSNCIAVHPGANRLLTAAHAEAARGAIEAARLVYGQFETALVAVEAAFAIAQAR
                     GVPTLLNPSPWQAPPEALRAATHTVIVNEVEAVDLLALPQPLDGTPQGAARAVAAALA
                     GFHAHWPAARRLVVTLGEHGALGAEFDDGRWSMWHAPARVIDAVDTLGAGDGFSSGYA
                     LAVLQGDALPMALAWGNLCGAHVAARTGVINALPDAQGLAAMIEQAPPAAARQLTFE"
     gene            complement(5478..6227)
                     /locus_tag="PKV30_01475"
     CDS             complement(5478..6227)
                     /locus_tag="PKV30_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL28923.1"
                     /translation="MSGSFTSLQLDTRAPEPLWRQLYSQLDDLLTSGAVQEGASLPPE
                     RDLAEALGVSRSTVKRSYDQLRHDKRVGGRGRAGSVVRTPPERVQPTLGQLKGFTEEM
                     QELGKFATSTLLVCEVVTDRRMASIFQRPSHARFLHIERIREADGVPMTRELAWYDLT
                     LAPAMAEWDGGGSAYERLRKVCGLALGGADQSIEAVLSSPGESAAFDFESPQPCLLFK
                     RMTRVITGALVEYVEGTFRGDAYVYRIELQP"
     gene            complement(6477..7334)
                     /gene="queF"
                     /locus_tag="PKV30_01480"
     CDS             complement(6477..7334)
                     /gene="queF"
                     /locus_tag="PKV30_01480"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902230.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HRL28924.1"
                     /translation="MTHPATPLDSPLGKATHYADQYDPALLFPIPRAPQRERIGIAGQ
                     PRFFGADLWTAFELSWLNLRGKPQLAIAHIMVPCESTHIVESKSFKLYLGSFNGTRIA
                     DAAEVRRRIQVDVSTAVWQGGPVQASVGVRLIAPEDFDREAVHELDGLYLDRLDIECE
                     PVPGPTPDLLTAAFDEQPVEETLTSRLLKSNCPVTGQPDWGSVQIRYAGPQIDQAALL
                     RYIISFRNHDEFHEHCVERMFSDLWARCRPSKLSVYARYTRRGGLDINPWRTSHPGQP
                     PSPVRTARQ"
     gene            complement(7331..8239)
                     /locus_tag="PKV30_01485"
     CDS             complement(7331..8239)
                     /locus_tag="PKV30_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL28925.1"
                     /translation="MNIEANGISIEIEDSGGSGPAVLLIMGLGGQLIHWPADFVQGLI
                     DAGYRVLRFDNRDSGLSAHFTSRGAVNIPWIATQAWLGRRPRVPYTLGDMGRDALGVL
                     DALGIERAHVVGLSMGAMIAQRVALLAPDRVLSLTSIMGSSGARGLLKPKRAVLRVAA
                     GKPRTGSDAALAQYYLRFLKAIASPAFPPSDEQLHAMFRLTNQRSAPQTEATYRQLAA
                     ILADGERAGLLARITVPTLVVHGADDPWVPPACGRDTAARIAGARLALIPDMGHDLVP
                     VGHPEIVRRVLGVLLPFLPSAATPSA"
     gene            complement(8300..9148)
                     /locus_tag="PKV30_01490"
     CDS             complement(8300..9148)
                     /locus_tag="PKV30_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRL28926.1"
                     /translation="MTQRITPELRQWIVDQAQAGHSAESVLQSMKTSGWDEDVAIDAM
                     ETTLRGHLNEQAVVQGLPPSSKVPEPDIADSPLYLDAGDRRVAVLSVMAAPRVIVFGG
                     LLSDEECDALIGAAKPRMSRSLTVATKTGGEEVNDDRTSQGMFFQRGENALVKTIEAR
                     IARLVNWPLENGEGLQILQYVPGTEYKPHYDYFDPAEPGTPTILKRGGQRVGTLVMYL
                     NEPEKGGGTTFPNVFFEVAPKRGNAVFFSYERPHPSTKTLHGGAPVVAGEKWIATKWL
                     REGEFR"
     gene            complement(9162..9935)
                     /gene="yaaA"
                     /locus_tag="PKV30_01495"
     CDS             complement(9162..9935)
                     /gene="yaaA"
                     /locus_tag="PKV30_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HRL28927.1"
                     /translation="MLFLLSPAKALDYDTPVPPTVQPTPPLFARQAAELIGVLRTRSP
                     QEIAELMHLSDALATLNVARYEAWRPRFTAKNSKPAVLAFNGDVYGGLDAQSLTAAQL
                     DWAQAHVCILSGLYGVLRPLDRMQPYRLEMGTRLQTDHGANLYQFWGDQIAQYLNQRL
                     KGDKAPTVVNLASQEYFKSVDRQALKARVIDCVFEDYKDGQYKIISFLAKRARGLMAR
                     YAVQHKVATPKKLEGFDLEGYAFAPAASEPDRLVFRRKA"
     gene            9934..10629
                     /locus_tag="PKV30_01500"
     CDS             9934..10629
                     /locus_tag="PKV30_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRL28928.1"
                     /translation="MLPILYQDPHLVAIDKPAGLLVHRSGLDAHETRFAVQLLRDQIG
                     RPVWPAHRLDKGTSGVLLFALDADTARALGQAFENGGAIAKTYRAMVRGCPADAGLID
                     HPLTRLDDDARGPLVAQAARTRFRTLSRLSLPLAYGPFAETRCALLELRPETGRRHQL
                     RRHCKHMAHPIIGDATHGKGPLNRALAEWLGLQRLWLHALSLELAHPWTGQHLRIEAP
                     PGSEWRRWPGLGG"
     gene            complement(10634..11479)
                     /locus_tag="PKV30_01505"
     CDS             complement(10634..11479)
                     /locus_tag="PKV30_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28929.1"
                     /translation="MIYIKTDKGRAALLQRDALNLRERQVMVLCNGARTLEELVELFG
                     DTVKEDVDRLDRRGLLAGQLKHSRPAELKAALAERPQMADAVSQAEAAHPPDSFVASR
                     LDTHHPDPPEPEALPRMSLEELTALASVPPLDPGAVAPVREALADAVSSPPADPAALG
                     QVAALVNSIDAEQVEMRSPLAAQVYMTQVLMALDTEPAMHLVETHGDVRHDVDILLYL
                     AQGLGLTHGAAGEDVALRVAMRVGRLLPETTLPMLLDCTLDYVPPGFSVLLYEFVLAG
                     RDTAA"
     gene            complement(11597..14176)
                     /locus_tag="PKV30_01510"
     CDS             complement(11597..14176)
                     /locus_tag="PKV30_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRL28930.1"
                     /translation="MATDIEKAPEALQQLVAETDTGGRKPTGATAKVIFALALAWALF
                     QYWYASPLPFVLGFGILNDTEARAIHLAFSFALAFLAWPAFRSSPRRHVPALDWVLAL
                     VGAFAGAYLMLFYRELATRPGQPTTMDIVVATTGLVLLLEATRRAVGWPMAALALLFL
                     AYCMGGPYLPDVLAHKGASLNRLLSHMWLTTEGVYGIALGVSASTIFVYVLFGALLDR
                     AGGGNYMMQVSFAALGHMRGGPAKVAVVSSALNGMISGSSVSNVVSGGIFTIPLMKKA
                     GYGGVKAGAIETMSSVNGQIMPPVMGAAAFLMVEYVGIPYSEVVKHAFLPASLSYLGL
                     LYIVHLEALKMGMQPIAARSPRTWHNRLLRNLIGITGSIVVVGVLYYLLQFFKSTLGA
                     AAPWAVGGTLLGMYLYALREAARCPDLPEDIDVNNPKPLETWPTVRAGLHFIIPIGVL
                     IWCLMVEEMSPALSAFWAVTALIVLMLTQRPLIDFFRGVKTPGAWLQGWDSVVQGFND
                     GARNMIGIGIATATAGIIVGAITLTGLGLRMTEFVEFVSHGNVMLMLLFIAFVCLVLG
                     MGVPTTANYVLVATLMAPVAVELGAQAGLVIPLIAVHLFVFYYGIMGDITPPVGLATF
                     AASAISGEDSIATGIQGALYALRTVILPFIWIFNPQLLLIDVHGWGELILVVTACSVA
                     MLVFAALTMNWFRIKLKWWEFALLTLSVALLFRPGFFMDRIAPEYTAAPADQVYEVAK
                     NLPGNGTLVMQIKGTTLEGDDVEKTVSVQLGDKGPDGRKRLADAGLTLVPLGTDMQVG
                     AVKFGSQAKRSGFEPGWDVSHVQVPTDRPTKYWFFLPGFLLAALVWWLQGLRMRQVGL
                     RPA"
     gene            complement(14310..15296)
                     /locus_tag="PKV30_01515"
     CDS             complement(14310..15296)
                     /locus_tag="PKV30_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRL28931.1"
                     /translation="MHMTVKLVLKSLALAAAASTALLAAPAQAQQKFVTIGTGGVTGV
                     YYAAGGAICRLVNKDRAKHGIRCSVESTGGSVFNVNTIKAGELDLGFTQSDVQYNAVK
                     GLNQFKDGGAVGDLRAVFAVHPEPFTVVARKEANIAKFEDFKGKRFNVGNPGSGTRAS
                     MEELLGAMGWKLNDFSLASELKADEHGPALCDGKIDGFFYGVGHPSANIQDPTTSCGA
                     KLVSLTGPAVDKLVADKPYYAKVTIPAGLYPNNPQATNTYGVLATIVASAKTPADTIY
                     AVTKAVFDNFDEFKKLHPALAHLKPEDMVKNGLSAPLHDGAARYYKEKGWIK"
     gene            complement(15317..16651)
                     /locus_tag="PKV30_01520"
     CDS             complement(15317..16651)
                     /locus_tag="PKV30_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL28932.1"
                     /translation="MSSTHVFHRNLLTTPPVAVRGEGIWIEDAAGKRYIDASGGAAVS
                     CLGHGHPDVLAAMHAQIDQLAYAHTSFFTTEAAEALAEQLVRTAPAGMSHAYLVSGGS
                     EAVEAALKMARQYYLEVGQPERSHFIARRQSYHGNTLGALAVGGNAWRRQQFAPLLID
                     VTHVAPCYPYRDQRTDETPEAYGQRLAQELDETIQRVGPGKVLAFIAETVGGATAGVL
                     VPVPGYFQAVREVCDKHAVLLILDEVMCGMGRTGTLHACEQDGVVPDLMTIAKGLGGG
                     YQPIGAVLAQKKIVDAMSQGSGFFQHGHTYLGHAVACAAALAVQQVIERDGLLQKVQR
                     GAPVLQALLAEKFKNHAHVGDMRGRGFFWGVELVQDRATQAPFDPARRLHAKVKAAAF
                     ARGLLVYPMGGTVDGRYGDHILLAPPFITTEADLTQIVDRLADAVDAAIAQG"
     gene            complement(16748..17251)
                     /locus_tag="PKV30_01525"
     CDS             complement(16748..17251)
                     /locus_tag="PKV30_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28933.1"
                     /translation="MTQATLTFRLLITSVLSLAAAQASAACYTVYDAAGRVVHQADAP
                     PASAAEAGTVPEGGRVMLNGAHCTSGGVAVAEQIMPAPEAARAPLLTDVHTAASARTP
                     YAVITRDIAVVAPANAEQVWQRAPLMATAGRVAPPATRRSSTETVITEWADGRVEVAR
                     GPLTARR"
     gene            complement(17443..18216)
                     /locus_tag="PKV30_01530"
     CDS             complement(17443..18216)
                     /locus_tag="PKV30_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HRL28934.1"
                     /translation="MTIKGKTALVTGSTSGIGLGIAIELAKQGANILMNGFGDVEAPK
                     KEIAAHGVKVDYHGADMSKPAEIEAMMKFAAEKFDGVDILVNNAGIQHTSSIRDFPTE
                     RWDAIIAINLTSAFHTTRLAIPHMVDKNWGRIINIASVHGLVASKDKSAYVAAKHGIV
                     GLTKVTALELATTGVTANAICPGWVLTPLVQKQIDARAEKDKISVEQATRELLSEKEP
                     SQQFTTPEQLGAMAVFFCSDAAANVRGQAWANDGGWTAQ"
     gene            18330..19430
                     /locus_tag="PKV30_01535"
     CDS             18330..19430
                     /locus_tag="PKV30_01535"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HRL28935.1"
                     /translation="MSVVKSITSPMKFLLQRDLLANLGALIEPYGPKAHLICDPFIAQ
                     LVKDKTAASFQQHQIAAEVTEFGGECSDDEIKRHQDAIAQGGGQYVVGVGGGKTLDTA
                     KAVAHYAQLPVVIIPTLASTDAPCTALSVIYNTDGSFNRYLFLPKNPDAVFADLSVLA
                     AEPARFFAAGVGDGLATYFEARTCYASQGDNLVLMKPSLAGLGIARLCFETIRDYAAE
                     ALQAVKAKAVTTALENTVEATIYMSGVGAESGGLAAAHAIHNGMTAVHDLHGAQHGEK
                     VAFGLVAQLVLEGAPRAELEQVIHIIKSVELPLTLADLGLKKFDEAEWRRAAELACAE
                     GETIHNEPFKVTPAMVYDAIVTADRLLQSYKD"
BASE COUNT         3250 a         6551 c         6500 g         3181 t
ORIGIN      
        1 actacaccga cttctacacc tcggtgcacc acgccaccaa catcggcaag cagtttcggc
       61 ccgacaaccc gctactgccc aactacaagt gggtgccgat cggctaccac ggccgctcgt
      121 cgagcatcgg catctcgggc caatccttca agcgccccaa gggccaggtc aagccgcccg
      181 acgccgacgc gccggtgctc aagcccagcg cgcgcatgga catcgagctc gaaatgggcg
      241 tcttcgtggg gcaagccaac gaactgggcg agccggtacc gatgaccgag gcggaagacc
      301 acatcttcgg cctggtgctg ttcaacgact ggtcggcgcg cgacatccag ccgtgggaat
      361 accagccgct cggccccttc ttgtccaaga atttcgcctc gacgatttcg ccgtggatcg
      421 tgacgatgga agcgctggcg cccttccgcg cgcccttcag ccatccggcc gaggacccgc
      481 agccgctgcc ctatctgtcg agcgctgaca acagcgcgca gggcggactc gacatccatc
      541 tcgaagccct gattgaaaca cccaagatgc gcgacgccaa agcagcgccg gcgcgcttca
      601 cgcagaccaa ctaccgccac gcctactgga cggccgcgca aatggtggcg caccacaccg
      661 tgaacggctg caacctgcaa cccggcgacc tgctgggcac cggcaccctg agcggcccca
      721 cgctcgacca agcctgcgcg ctgatcgaac tgaccaccgg cggcaagaac ccgatccagt
      781 tgccgaacgg tgaaagccgc acctggctgg aagatggcga caccgtcatc ctgcgtggtt
      841 ggtgcgaaaa acccggcgcc gcgcgcatcg ggtttgggga gtgtgtgggg acggtgttgc
      901 cggcgacttg agcgctcatt gacggccgat cttgacttga ttaaactagt catctaactg
      961 gtttgatcgg agatcgacat gcacgcttgg cctgtgcaag atgcgaaagc gcgcttcagc
     1021 gagttcctgg atgcctgcct gtcggacggg ccgcagctgg tcacgcggcg tggtgccgaa
     1081 gccgcagtgc tggtgcccat gcacgaatgg cggcgcctgc agcgcgccgc gcggccgtcg
     1141 ttgaaggagt tgctgttgac cgacgaggcc cgcgccgacc tggtgctgcc cgcgcgcggc
     1201 aaggcacggc ggcgcccggt ggcggtaccg gactgatgta tctgctcgac accaacgtcg
     1261 tgtccgagtt gcgccggccg cgcccccatg gcgccgtgct ggcctggctg caagccgtag
     1321 acgacgcgca actgttcatc tcagccgtca ccctgggcga gatccaggcc ggcatcgaat
     1381 tgacacgcga acaggacacc gacaaggcga gcgagattga agcctggctc gatcaagtcg
     1441 ctgctgccta caacgtgctg ccgctggacg cggcagcgtt tcgcgagtgg gcacgactca
     1501 tgcaccgccg ctcggacacg ctgtacgaag acgccatgat cgccgccacg gcgcgcgtgc
     1561 atggcctgac agtggtgagc cgcaatgtgg ccgacttcaa ggcgctgggc atggaggccc
     1621 tcaacccctt cacggcgcgc tgactgcacc gcgaagttcc gtccatgcgc cgcatcgccc
     1681 acctcgacat ggacgcgttc tacgcgtcgg tcgagtggct gcgctacccg cagttgaagg
     1741 ggctgccgat ggtgatcggc ggtgggcggc gcaaggagga tgacctgatc gggcgcctga
     1801 acgccgagcg gcccgagcgc cactggacca gcgccgacct ggccgatatc ccggtcgact
     1861 tcttccccct gctgcgcgac tacgtcggcc gcggcgtcat caccaccgcc acctacgcgg
     1921 cgcggccgtt cggcatcggc tcggccatgg gcttgatgaa ggccgcgaaa ctctgcccgc
     1981 aggccatctt gctgccggtc gatttcgacc agtaccgcaa atattcgcgc gccttcaagc
     2041 aggtcatccg cgacatcgcg ccggtgatgg aagaccgtgg cgtggacgag gtgtacatcg
     2101 acttcaccga cgtgcccggc ggccagcgcg agggcgggcg tgtgctggcg cggctgatcc
     2161 agaaaagcat tgccgacacc acggggctga cctgctcgat cggcgtggcg cccaacaagc
     2221 tgatcgccaa gatggcgagc gagttcaaca agccgaacgg catcagcatc gtgttcgagc
     2281 acgaccttga gcgcctgatc tggccgctgc cgtgccgcaa ggtcaacggc gtcggcccca
     2341 aggcggatgc caagttgcaa ggccacggca tccacaccat cggcgagctg gcggcgcgcg
     2401 agcgggcctg gctgattcag cacttcggca aagcctacgg cgcctggctg cacgacatgg
     2461 cctggggccg cgacgagcgc ccggtcgtga ccgagagcga acccgtcagc atgagccgcg
     2521 aaaccacctt cgagcgcgac ctgcacgcgg tgcgcgacaa ggcggctttg agcgccatct
     2581 tcaccacgct gtgccagcag gtggcgggcg atctgcaacg caagggctac gtcggcaaga
     2641 ccatcggcat caagctgcgc tttgacgatt tcaagatcgc cacgcgcgac cagacggtgg
     2701 ccgaacacac ggccgacgca cagcgcatcc gccaggtggc gggccagtgc ctgaagcgcg
     2761 tgccgctgga tcgccggctg cggctgctgg gtgtgcgcgt gggatcgctg gcgaggcagt
     2821 ctgacttggc ccgtggtggt gattcagagc caaatctggc gctgccgctt ttccagtaag
     2881 cgcgagcagc tatctttttt gatactgaac acgcaatcag tgcgctgcac gcctgtggga
     2941 gcggccttgg ccgcgatcgg cctcccacga tgaccaatcg tccatcgcgg gcaagcccgc
     3001 tcccacccgc gcgagtcaca cccgtcgcgc gcgagacgat gtttcaaggg cgcgctgcac
     3061 tacagtgacg ccatgaacac cgccgccctc acctgctttc aactcaccgt caccgaccac
     3121 atcgcgcacc tggtgctgag ccggcccgaa gcgatgaaca ccatgggccc gaccttctgg
     3181 cgcgagctgg atcaggtgct gacccgcctc aatcagagcg gcgaggcgcg cgtgctggtg
     3241 gtctccagca ccggcaagca tttctcggcc ggcatggcgc tcgacgtgtt tggcggcgcg
     3301 atcagcatgg acgacgccac gcccgaaggc cgcgccgcca tcgccgacct gctgggcggg
     3361 ctgcaggcca ccttcaccag gctggaaacg ctgcgcatcc ccgtcatcgc ggccatccag
     3421 ggcggctgcg tgggcggcgc ggtggatctg gtcacggcca tgtgcgtgcg ctacgccacg
     3481 cacgacgcgt tcttcgtcat tcaggaaatc aacatcggca tggtggccga cgtcggcacg
     3541 ctgcagcgct tgcccaagct ggtgccgctg gcggtggtca aggaactggc ctacaccggc
     3601 cgccgcctgc cggccgccaa ggcgcgcgat tacgggctgg ttaacgatgt gttcgacacg
     3661 cctgaggcct gcctggcggc ggcgatgcaa tgcgccaagg agatcgtcgc caagccgccg
     3721 gtcgccatct ggggcaccaa gcaggtgatc cactacgcgc gcgaccattc ggtggacgat
     3781 gcgctgcgcc aaatgggttg ggtacagggc gcgatctgga gcaacgcgca cgtgcgcgag
     3841 gcggtgacgg cgatgaagac gcagcgtgaa gcgcagtttc cgtcgctggc cgaattgaag
     3901 ggctttggtg ccgaataggc cgctggcgct tgtcaggcaa gcgccagcag ctatcttttt
     3961 atgaccgcgc ctgcggcgca tgacctcggc ctgcggccat ccatgacagc gctgcgcgcc
     4021 atgacccaca cttggtcatt tcgtcataga agcgcagcga agtcatttcg tcattgaatc
     4081 tacggcatgg cggcgagaaa caagcgtcag gccgcccgct gctcaaagcg cagtttcatt
     4141 accgtgatgg ccccggtcag caccaccatc accgcgttcg ccaccatcag cggcatgtcg
     4201 ccgcgcagcc agccgtagac cagccacagc gccagcccga tggtgatgat cacgtaggtg
     4261 ggcagggaga tgccggacac gtcgcgcgtc ttccaggtgc gccagacctg gggtacgaag
     4321 gaggacgtgg tgaaacaggt ggccaccagg cccagcaaat ccagcgcgtt catttgcttt
     4381 ccttaatctc gtggggtttt tgattttcgc ggcaagctgt catgctcagc gcaagacccc
     4441 gattggaccc gattgtcgtg accacgccgc ccctcccccg cgcctttgtg ctgggcaatt
     4501 tcgtgcaagc ctgctgctgg cacgtggcgc gcctgccgca ggccggcgaa acgctggagg
     4561 cgcaccacct gcagatcgaa ccgggcggca aggggttgaa cgtggccgtg tgcctgcagc
     4621 gcctgggcgt ggcggtggac acgctgatcg gctgcggcga cgacgcggcc ggcgacgcct
     4681 tgctggcgct gctggcgcgc gaaggcctgg cgacgacgca cgtgcaccgg ctggccggcc
     4741 cgtcgggctg gggcgccggg ctgatcggcg ccgacggcag caactgcatt gcggtgcacc
     4801 cgggcgccaa ccggctgctc accgctgccc atgccgaggc ggcgcgcggc gccatcgagg
     4861 cggcgcgcct ggtttatggc caattcgaga ccgcgctggt tgcggtcgaa gccgccttcg
     4921 ccatcgccca ggcgcgtggc gtgcccacgc tgctcaatcc atcgccctgg caagcgccgc
     4981 ccgaggcgct gcgcgccgcc acgcacaccg tcatcgtcaa cgaggtcgag gcggttgatc
     5041 tgctggcgct gccgcagccg ctggatggca cgccgcaggg tgccgcgcgc gccgtggcgg
     5101 cggcgctggc gggctttcac gcgcactggc cggcggcgcg tcggctggtg gtcacgctgg
     5161 gcgaacacgg cgcgctgggc gctgaattcg atgacgggcg ctggtcgatg tggcacgcgc
     5221 cagcgcgcgt catcgacgcg gtagacacgc tgggcgcggg cgatggtttc tcaagcggct
     5281 atgcgctggc cgtgctccag ggcgacgcgc tgccgatggc gctggcctgg ggcaatttgt
     5341 gcggggccca tgtggcggcg cgcaccggcg tcatcaatgc actgccggat gcgcaaggcc
     5401 tggccgcgat gatcgaacaa gcgccgccgg ccgcggcccg tcagctcact tttgaatagc
     5461 tgcccgcgct ggccccgtca gggctgcagc tcgatgcgat acacataggc atcgccgcgg
     5521 aaggtgcctt cgacgtattc gaccagcgcg ccggtgatca cgcgcgtcat gcgcttgaac
     5581 agcaggcagg gctgtggcga ctcgaagtcg aacgcggccg actcgccggg cgagctgagc
     5641 accgcctcga tcgactgatc ggcgccgccc agcgccaggc cgcacacctt gcgcagccgc
     5701 tcgtaggccg agccgccgcc gtcccattcg gccatggcag gcgccagcgt caggtcgtac
     5761 caggccagct cgcgcgtcat gggcacgccg tcggcctcgc ggatgcgctc gatgtgcaga
     5821 aagcgcgcgt gcgacgggcg ctggaagatc gacgccatgc ggcgatccgt caccacctcg
     5881 cacaccagca gcgtgctggt ggcgaacttg cccagctcct gcatttcctc ggtgaagccc
     5941 ttgagctggc ccagcgtggg ctgcacgcgc tcgggcggcg tgcgcaccac gctgccggca
     6001 cggccgcggc cgcccacgcg cttgtcgtgg cgcaactggt catagctgcg cttgacggtg
     6061 gagcggctca cgcccagcgc ctcggccagg tcgcgctcgg gcggcaggct ggcgccttcc
     6121 tgcacggcgc cggacgtcag caggtcatca agttgggaat agagctggcg ccacagcggt
     6181 tcgggcgcac gggtgtcgag ctggagcgac gtgaaactgc cggacatgga tatcaagacc
     6241 tcgcgatact tccaacgaac aggttgatga tgtcgtctgc acctgggacc agtgaacccg
     6301 aagcgaacga ccgtgccgga ttatcacggc aaagccatgc cactcggaac gcccgtgggt
     6361 gtttgggcgc cacatccacg ccatgacgcg cattctgtcg cgccgatgcg gctttttgaa
     6421 tgaatgcagc cgctggcgca ggtgcggaaa gcgcggccag ctacgaattc aggagtctac
     6481 tggcgcgcag tgcgcaccgg tgacggcggc tggccggggt ggctggtgcg ccaggggttg
     6541 atgtccagcc cgccgcggcg cgtgtagcgc gcgtagaccg acagcttgct aggcctgcag
     6601 cgcgcccaca ggtcgctgaa catgcgctcg acgcaatgct cgtgaaactc gtcgtggttg
     6661 cgaaaactga tgatgtagcg cagcagcgcc gcctggtcga tctgcgggcc ggcgtagcgg
     6721 atctgcacgc tgccccagtc gggctggccg gtgacggggc aattgctttt cagcaagcgg
     6781 ctggtcagtg tttcttccac cggctgctcg tcgaaggcgg ccgtcagcag atcgggcgtc
     6841 gggccgggca cgggctcgca ttcgatgtcg aggcgatcga ggtagaggcc gtccagctcg
     6901 tgcaccgcct cgcggtcgaa gtcttccggc gcgatgaggc gcacgccgac cgaggcctgc
     6961 accggcccgc cctgccagac ggcggtgctc acatcgacct ggatgcggcg ccgcacctcg
     7021 gcggcgtcag cgatgcgcgt gccgttgaag ctgcccaggt aaagcttgaa ggatttgctt
     7081 tcgacgatgt gcgtgctctc gcacggcacc atgatgtggg cgatggccag ttgcggcttg
     7141 ccgcgcaggt tgagccacga cagctcgaag gccgtccaca ggtcggcgcc aaaaaagcgc
     7201 ggctggccgg cgatgccgat gcgttcacgc tgtggcgcgc gcggaatggg aaacagcagc
     7261 gccgggtcgt actggtcggc gtagtgcgtg gccttgccca agggcgaatc gagcggcgtg
     7321 gcggggtggg tcatgcggag ggcgtggcgg cggagggcag aaacggcagc agcacgccca
     7381 gcacgcggcg cacgatttca gggtggccga ccggcaccag gtcgtgcccc atgtcgggaa
     7441 tcagcgccag gcgcgcgccg gcaatgcgcg cggcggtgtc gcgcccgcag gccggcggca
     7501 cccacgggtc gtcggcgccg tgcaccacca gcgtcggcac cgtgatgcgg gccagcaggc
     7561 cagcgcgctc gccatcggcc aggatggcgg ccagctggcg gtaggtggcc tcggtctgcg
     7621 gggcgctgcg ctggttggtg aggcgaaaca tggcgtgcaa ctgctcgtcg ctgggcggaa
     7681 aggcggggct ggcaatggcc ttgagaaagc gcaggtagta ctgcgccagc gccgcgtcgc
     7741 tgccggtgcg cggcttgccg gcggccacgc gcagcaccgc ccgcttgggc ttgagcaggc
     7801 cgcgcgcgcc gctgctgccc atgatcgagg tcaggctcag cacgcggtcg ggcgccagca
     7861 gcgccacgcg ctgggcgatc atggcgccca tgctgaggcc gaccacgtgg gcgcgctcga
     7921 tacccagcgc gtccagcacg ccgagcgcgt cgcgccccat gtcgcccaac gtgtagggca
     7981 cgcgcgggcg ccggccgagc catgcttgcg tggcgatcca cgggatgttg accgcgccgc
     8041 gcgacgtgaa gtgcgccgac aggccgctgt cgcggttgtc gaagcgcagc acgcggtagc
     8101 cggcatcgat cagcccttgc acgaagtccg ccggccagtg gatcaactgc ccgcccaggc
     8161 ccatgatgag cagcaccgcc gggccgctgc cgcccgaatc ttcgatttcg atggagatgc
     8221 cgtttgcttc tatattcata gctgcttgcg cttgtccatc aagcgcttgg ggcctatttc
     8281 attaaaaaaa cgtgcccgct caacgaaact cgccttcgcg cagccacttg gtggcgatcc
     8341 acttctcgcc cgccaccacg ggcgcgccgc cgtgcaaggt cttggtgctg gggtgcggtc
     8401 gctcgtagct gaagaacacc gcgttgccgc gcttgggcgc gacttcgaaa aacacgttgg
     8461 gaaaggtggt gccgccgcct ttttcaggct cgttcaggta catcaccagc gtgcccacgc
     8521 gctggccgcc gcgcttgagg atggtgggcg tgccgggctc ggcggggtcg aagtagtcgt
     8581 agtgcggctt gtattcggtg cccggcacgt actgcaggat ttgcaggcct tcgccgtttt
     8641 caagcggcca gttgaccaac cgggcgatgc gcgcctcgat ggtcttcacc agcgcgttct
     8701 cgccacgctg aaagaacatg ccctggctgg ttcggtcgtc gttgacctct tcgccgccgg
     8761 tcttggtggc gacggtgagc gagcggctca tgcgcggctt ggccgctccg atcagcgcat
     8821 cgcactcttc gtcggacagc aggccgccga acacgatgac gcgcggcgcg gccatcacgc
     8881 tcagcaccgc cacgcggcgg tcgccggcat ccaggtacag cggcgaatcg gcgatgtcgg
     8941 gctcgggcac cttcgacgac ggcggcaggc cctgcaccac cgcctgctcg ttcaggtggc
     9001 cgcgcaaggt ggtttccatg gcgtcgatgg cgacgtcctc gtcccagccc gaggtcttca
     9061 tcgattgcag caccgattcg gcgctgtgcc cggcctgggc ctggtcgacg atccattggc
     9121 gcaattcagg ggtgattcgc tgtgtcatgg caacggggtg ttcaagcctt gcggcggaaa
     9181 acaaggcggt cgggctcgga cgccgcgggc gcgaaggcgt agccctccag gtcgaagcct
     9241 tcgagctttt tgggcgtggc gaccttgtgc tgcacggcgt agcgcgccat caacccgcgc
     9301 gcccgcttgg cgagaaagct gatgatcttg tactggccgt ccttgtagtc ctcgaacacg
     9361 cagtcgataa cgcgcgcttt cagcgcctgg cggtcgaccg acttgaagta ttcctgcgac
     9421 gccaggttga ccacggtggg cgccttgtcg cccttcagcc gctggttcag gtactgcgcg
     9481 atctgatcgc cccagaactg gtagaggttg gcgccgtgat cggtctggag gcgcgtgccc
     9541 atctccagcc ggtagggctg catgcggtcg agcgggcgca gcacgccgta caggccgctc
     9601 aggatgcaga cgtgggcttg cgcccagtcg agttgcgcag cggtcaggct ttgggcatcc
     9661 aggccgccat agacatcgcc gttgaaggcc agcacggccg gcttcgaatt cttggcggtg
     9721 aagcgcggcc gccacgcctc gtagcgcgcc acattcagcg tggccagcgc gtcgctcagg
     9781 tgcatcagtt cggcaatctc ttgtggcgac cgggtgcgca gcacgccgat caactcggcc
     9841 gcctggcgcg cgaagagcgg cggcgtgggc tgcaccgtgg gcggcaccgg cgtgtcgtaa
     9901 tccagcgcct tggccgggga caatagaaac agcatgctgc cgattttgta ccaagacccg
     9961 cacctggtcg ccatcgacaa gcctgccggc ctgctggtgc accgcagcgg gctggacgcg
    10021 catgaaacgc gctttgccgt gcagctgctg cgcgatcaga tcggccgccc ggtgtggccc
    10081 gcgcaccggc tcgacaaggg caccagcggc gtgctgctgt ttgcgctgga cgcggacacg
    10141 gcgcgcgcgc tgggccaggc tttcgaaaac gggggcgcca tcgccaagac ctaccgcgcc
    10201 atggtgcgag gctgtccggc ggatgcgggc ctgatcgatc acccgctgac gcgcctggac
    10261 gacgacgcgc gcgggccgct ggtcgcgcaa gcggcgcgca cgcgctttcg caccctgtcg
    10321 cgcctgagcc tgccgctggc ctacggcccg ttcgccgaaa cgcgctgcgc gctgctggaa
    10381 ttgcggcccg agaccgggcg gcgccaccag ttgcgccgcc actgcaagca catggcgcac
    10441 cccatcattg gcgatgccac gcacggcaag gggccgctca atcgagcgct ggccgagtgg
    10501 ttggggctgc agcggctgtg gctgcacgcg ctgtcgctgg aactggcgca cccctggacg
    10561 ggccagcatc tgcgcattga agcgccgccc ggcagcgaat ggcggcgctg gcccgggctt
    10621 ggaggctagc gcgctacgcg gccgtatcgc ggccggccag cacgaattcg tacagcagca
    10681 cgctgaagcc gggcggcacg taatccagcg tgcagtcgag cagcatgggc agtgtggtct
    10741 cgggcagcag gcggccgacg cgcatcgcca cgcgcaaggc tacgtcttcg cccgccgcgc
    10801 catgggtcaa gcccagcccc tgcgccaggt agagcaggat gtcgacgtcg tgccgcacat
    10861 cgccgtgcgt ttcaaccagg tgcatggccg gctcggtgtc cagcgccatc agcacctgcg
    10921 tcatgtagac ctgcgcggcc agcggcgagc gcatttcgac ctgctcggcg tcaatgctgt
    10981 tgaccagcgc ggccacctgg ccaagcgcag cgggatcggc gggcggcgac gagaccgcat
    11041 cggccagcgc ttcgcgcacc ggcgccacgg cgcccgggtc gagcggcggc accgaggcca
    11101 gcgcggtcag ctcttcaagc gacatgcgcg gcagcgcttc gggctcgggt gggtccgggt
    11161 gatgggtgtc aagacggctg gcgacgaagc tgtcgggcgg gtgggccgct tcggcctgcg
    11221 acacggcatc ggccatctgc gggcgctcgg ccagcgcggc cttgagttca gcgggccggc
    11281 tgtgcttgag ctggccggcc agcaagccgc ggcggtccag gcgatccacg tcttccttga
    11341 cggtgtctcc aaacagttcc accagttctt ccagcgtgcg cgccccgttg caaagcacca
    11401 tcacctgccg ctcgcgcagg ttcaaggcgt cgcgctgcag tagcgcggca cggcccttgt
    11461 cggtcttgat gtaaatcatg aatctgaaag attgaggtaa gcgcgaatta ttgcagcctg
    11521 ttgtatgcag tgtgcggcaa cgcagtcttc cgcacgactg ccgtcaactc atgaacaagg
    11581 gtgtttgcgc cacgcatcac gccggacgca agcccacttg ccgcatgcgc agcccctgca
    11641 accaccacac cagcgccgcc agcagaaagc cgggcaggaa gaaccagtac ttggtcggcc
    11701 ggtccgtcgg cacctgcacg tggctcacgt cccagcccgg ctcaaagccc gagcgcttgg
    11761 cctgggagcc gaacttgacg gcaccgacct gcatatcggt gcccagcggc accagcgtca
    11821 agcccgcatc ggccaggcgc ttgcgaccgt ccgggccctt gtcgcccagt tgcaccgaca
    11881 cggttttctc gacatcgtca ccctccagcg tggtgccctt gatctgcatc accagcgtgc
    11941 cgttgcccgg caggtttttc gccacctcgt acacctgatc ggccggcgcg gcggtgtatt
    12001 cgggcgcgat gcggtccatg aagaagccgg ggcggaacag caacgccacc gacagcgtca
    12061 gcagcgcgaa ctcccaccac ttcagcttga tgcgaaacca gttcatcgtc agcgccgcga
    12121 acaccagcat ggcgaccgag caagctgtga cgaccaggat caattcgccc cagccatgca
    12181 cgtcgatcaa cagcagttgc gggttgaaga tccagatgaa gggcaggatc accgtgcgca
    12241 gcgcatagag cgcaccctgg atgccggtgg caatcgaatc ctcgcccgag atggccgagg
    12301 cggcaaaggt cgccagcccc accggcggcg tgatgtcgcc catgatcccg taataaaaca
    12361 cgaacaaatg cacggcgatg agcggaatca ccagccccgc ctgcgcgccc agttcgaccg
    12421 ctaccggcgc catcagcgtc gccaccagca cgtagttggc cgtggtcggc acgcccatgc
    12481 ccagcaccag gcagacgaag gcgatgaaca gcagcatcag catcacgttg ccgtggctga
    12541 cgaattcgac gaactccgtc atgcgcaggc ccaggcccgt gagcgtgatg gcgccgacga
    12601 tgatgcccgc cgtcgctgtg gcaatgccga tgccgatcat gttgcgggcg ccgtcgttga
    12661 agccctgcac caccgaatcc cagccttgca gccaggcgcc gggcgtcttc acgccgcgaa
    12721 agaaatcgat caagggccgc tgcgtcagca tcagcacgat cagcgccgtg acggcccaga
    12781 aggctgagag cgcgggcgac atctcttcca ccatcagaca ccagatcagc acgccgatgg
    12841 gaatgatgaa gtgcagcccc gcgcgcaccg tgggccaggt ctccagcggc ttggggttgt
    12901 tgacgtcgat gtcttcgggc agatcggggc agcgcgccgc ctcgcgcagc gcatataggt
    12961 acatccccag cagcgtgcca cccacggccc agggcgccgc cgcgcccagc gtgctcttga
    13021 agaactgcaa taaatagtag agcaccccga ccaccacgat cgagccggtg atgccgatca
    13081 ggttgcgcag caagcggtta tgccaggtgc gcggggaacg cgcggcgatg ggttgcatgc
    13141 ccatcttcag cgcctccagg tgcacgatgt agagcaggcc caggtacgac agcgacgccg
    13201 gcaagaacgc gtgcttgacg acttcggaat acggaatgcc gacgtactcc accatcagga
    13261 aggccgcggc gcccatcacc ggcggcatga tctggccgtt gaccgaactc atggtctcga
    13321 tggcgccggc cttaacgccc ccatagcccg ccttcttcat cagcggaatg gtgaagatgc
    13381 cgcccgacac cacattggag accgacgagc ccgaaatcat gccgttgagc gccgacgaca
    13441 ccaccgccac cttggccggc ccgccgcgca tatggccaag cgccgcaaag ctcacctgca
    13501 tcatgtagtt gccgccgccc gcgcgatcga gcagcgcgcc gaacagcaca tagacaaaga
    13561 tggtgctggc cgatacaccc agcgcgatgc cgtacacgcc ctccgtcgtc agccacatgt
    13621 gcgagagcag gcgattgagc gatgcgcctt tgtgcgccag cacgtcgggc agataaggcc
    13681 cgcccatgca ataggccagg aacagcagcg ccagcgccgc catcggccag ccgacggcgc
    13741 ggcgcgtggc ttcgagcagc agcaccaggc cagtggtggc gaccacgatg tccatcgtcg
    13801 tcggctgccc cgggcgcgtg gccagctccc gatagaagag catcaaatac gcaccggcaa
    13861 aggcgccgac gagggccagc acccaatcca gcgcaggcac gtggcgccgc ggcgacgagc
    13921 ggaacgccgg ccaggccaga aacgccagcg cgaacgagaa cgccaggtga atcgcccgcg
    13981 cctcggtgtc gttgaggatg ccgaagccca gcacgaaggg cagcggcgat gcgtaccagt
    14041 actggaacag cgcccaggcc agcgccagag cgaagatcac cttggccgtg gcgcccgtcg
    14101 gtttgcggcc gccggtgtcg gtctcggcca ccagttgctg cagcgcttcg ggggcttttt
    14161 cgatgtcggt ggccatttgg atactatatt ttttatagct gcttgcgctt gtacagcgtg
    14221 cgctggaggc ctgaaacact catgaactcc atcccatgga aaaaggcgaa gtcgccttcg
    14281 cctttttctg cggattcagc aagcgttgct tatttgatcc agcctttttc cttgtagtag
    14341 cgcgccgccc catcgtgcag cggcgcggag aggccgttct tgaccatgtc ctcgggcttg
    14401 aggtgcgcca gcgccgggtg cagcttcttg aattcgtcga agttgtcgaa cacggccttg
    14461 gtcacggcgt aaatggtgtc ggccggcgtc ttggccgacg ccacgatggt cgccagcacg
    14521 ccgtaggtgt tggtggcctg cgggttgttc gggtacaggc cggcggggat ggtgaccttg
    14581 gcgtagtagg gcttgtccgc caccagcttg tcgacggccg ggccggtgag cgacaccagc
    14641 ttggcgccgc agctcgtggt cgggtcctgg atgttggcgc tggggtggcc cacgccgtag
    14701 aagaagccgt cgatcttgcc gtcgcacagg gccgggccgt gctcgtcggc tttcaactcg
    14761 gaggcgagcg aaaagtcgtt cagcttccag cccatggcgc ccagcagttc ttccatcgac
    14821 gcgcgcgtgc ctgagccggg gttgccgacg ttgaagcgct tgcccttgaa gtcctcgaac
    14881 ttggcgatgt tggcctcttt gcgcgccacc acggtgaagg gctcggggtg cacggcgaac
    14941 acggcgcgca ggtcgccgac ggcgccgccg tccttgaact ggttcaggcc cttgacggcg
    15001 ttgtactgca cgtcggactg ggtaaagccc agatccagct cgcccgcctt gatggtgttg
    15061 acgttgaaca ccgagccgcc ggtcgattcg accgagcagc ggatgccgtg cttggcgcgg
    15121 tccttgttga ccaggcggca gatggcgccg cccgccgcgt agtacacgcc ggtgacgccg
    15181 cccgtgccga tggtgacgaa cttctgctgc gcctgcgccg gggcggccag cagcgccgtg
    15241 ctggcggcgg cggccagcgc cagggatttc agaaccagtt tgactgtcat gtgcattgat
    15301 ctcctgagaa aaaagttcaa ccctgcgcga tcgccgcgtc caccgcgtcg gcgagccgat
    15361 cgacgatctg cgtcaaatcg gcctcggtcg tgatgaacgg cggcgccagc aggatgtggt
    15421 caccataacg gccgtccacc gtgccgccca tgggataaac cagcaaaccg cgcgcgaaag
    15481 ccgccgcttt caccttggca tgcaagcggc gtgccggatc gaatggcgct tgggtggcgc
    15541 ggtcttgcac cagttcgacg ccccagaaga agccgcgccc gcgcatgtcg cccacgtgtg
    15601 cgtgattctt gaacttttcg gccagcagcg cttgcaggac gggcgcaccg cgctgcactt
    15661 tttgcagcaa gccgtcgcgc tcaatgacct gctgcacagc cagcgcggcg gcgcaggcca
    15721 cggcgtgacc caagtaggtg tggccgtgct gaaagaagcc gctgccctgc gacatggcat
    15781 cgacgatttt tttctgcgcc agcaccgcgc caatcggctg atagccgcca cccaggccct
    15841 tggcgatggt catcaggtcg ggcaccacgc cgtcctgctc gcaggcgtgc agcgtaccgg
    15901 tgcggcccat gccgcacatc acctcgtcca ggatcagcag cactgcatgc ttgtcgcaga
    15961 cttcgcgcac ggcctggaag tagcccggca ccggcaccag cacgccggcg gtggcgccac
    16021 ccaccgtttc ggcgatgaag gccagcacct tgccggggcc gacgcgctgg atcgtttcgt
    16081 ccagctcctg cgccagccgc tgcccgtagg cttcaggcgt ttcatcggtg cgctgatcgc
    16141 ggtaggggta gcatggcgcc acgtgggtga cgtcgatcag cagcggcgca aactgctggc
    16201 gccgccaggc gttgccgccc acggccagcg cgcccagcgt gttgccgtgg tagctctggc
    16261 gccgggcgat gaagtggctg cgctcgggct ggccgacttc gaggtagtac tgccgcgcca
    16321 tcttcagcgc cgcctccacc gcctcggagc cgccgctgac gagataggcg tggctcatgc
    16381 cggcgggcgc ggtgcgcacc agttgctcgg ccaatgcttc agccgcttcg gtggtgaaga
    16441 aactggtgtg cgcataggcc aactggtcga tctgcgcgtg catggcagcc agcacgtcgg
    16501 ggtggccgtg gcccaggcac gacaccgccg caccgcccga ggcatcgatg tagcgcttgc
    16561 ctgccgcgtc ttcgatccag atgccctcgc cgcgcacggc aacgggcggc gtggtcaaca
    16621 gattgcggtg aaaaacatgc gtgctgctca tcttggcgtt ccctcggcgt cggtcagcat
    16681 ggccgacgca gcaaggctag cacgcagcgg ccgccgacag cagacgcgaa aacaccaagg
    16741 ccgcgcttca gcggcgggcg gtcagcgggc cccgcgcgac ctcgacgcgg ccgtcggccc
    16801 attcggtgat cacggtttcg gtcgacgaac ggcgcgtggc cggcggcgcc accctgccgg
    16861 cggtcgccat caagggcgcg cgttgccaga cctgctcggc gttggccggc gccaccacgg
    16921 cgatgtcgcg cgtgatcacc gcgtacggtg tgcgtgcgct cgccgcggta tgcacgtcgg
    16981 tcaacagcgg tgcgcgggca gcctcaggcg ccggcatgat ctgttcggcc accgccacgc
    17041 cgcccgacgt gcaatgcgcg ccgttcagca tgacacggcc accctcgggc acggtgccgg
    17101 cttcggctgc gctggccggc ggcgcatcgg cctgatgcac cacgcgcccc gcggcgtcgt
    17161 acaccgtgta gcaagccgcg ctggcctgcg ctgcggccag cgacagcacg gaagtgatga
    17221 gcaatcgaaa ggtcagcgtc gcttgggtca tggtgggctc ccgcggcgcg ccttcgcgcc
    17281 gtaccgccat gttggcttcg cacgccaggc ccgcacgagt cggcgctgca ccggcgtgtc
    17341 ggccgccgcc gacagcgcac gcaaaaaagc gccgacaact acaatttcag tagctgccgg
    17401 cgcttgtctg gcgtgcgcca gaggccgatt cggcttgaaa gcttactgcg ccgtccagcc
    17461 gccgtcgttg gcccaggcct gtccgcgcac gttggcggcc gcatcgctgc agaagaacac
    17521 cgccatggcg cccagctgct cgggcgtggt gaattgctgc gacggctcct tctcgctcag
    17581 cagttcgcgc gtggcttgct cgacactgat cttgtccttc tcggcgcgcg cgtcgatctg
    17641 cttttgcacc agcggcgtca gcacccagcc cgggcaaatg gcgttggcgg tgacgccggt
    17701 ggtcgccaat tccagcgccg tcaccttggt caggccgacg atgccgtgct tggccgccac
    17761 gtaggccgac ttgtccttcg acgccaccag gccgtgcacc gaggcgatgt tgatgatgcg
    17821 gccccagttc ttgtccacca tgtgcggaat cgccagccgc gtggtgtgaa aggccgaggt
    17881 gaggttgatg gcgatgatgg cgtcccagcg ctcagtggga aagtcgcgga tgctcgacgt
    17941 gtgctgaatg ccggcgttgt tgaccaggat gtccacgccg tcgaatttct cggcggcgaa
    18001 cttcatcatc gcttcgatct cggccggctt gctcatgtcg gcgccgtggt agtcgacctt
    18061 gacgccgtgc gcggcgatct ccttcttcgg cgcctcgacg tcgccaaagc cgttcatcag
    18121 gatgttggcg ccctgcttgg ccaactcgat cgctatcccc aagccaatgc cgctggtgga
    18181 gcctgtcacg agtgccgtct ttcctttgat cgtcatcaat ttctcctgta atgggtgcaa
    18241 cctttccatc ataagttggc tcggcttctg caagcgtccc gaagacgcga cttcgcccca
    18301 tttcaacgcc ccaaaggagt gcattcacca tgtccgtcgt caaatcgatc acctcgccga
    18361 tgaagtttct gctgcagcgc gacctgctcg ccaacctggg cgcgctgatc gagccctatg
    18421 gtcccaaggc ccatctgatc tgcgacccgt tcatcgctca gctggtcaag gacaaaaccg
    18481 ccgccagctt ccagcagcac cagatcgcgg ccgaagtgac cgaattcggc ggcgaatgct
    18541 ccgacgacga aatcaagcgg caccaggacg ccatcgccca aggcggcggc cagtacgtgg
    18601 tcggcgtggg cggcggcaag acgctggaca ccgccaaggc cgtggcgcat tacgcccagc
    18661 tgccggtggt catcatcccc acgctggcct cgaccgacgc gccctgtacc gcgctctcgg
    18721 tcatctacaa caccgatggc tcgttcaacc gctacctgtt cctgccgaag aaccccgatg
    18781 ccgtcttcgc cgacctgagc gtactggcgg ccgagccggc gcgctttttc gccgccggcg
    18841 tgggcgacgg gctggccacc tacttcgagg cgcgcacctg ctacgcctcg caaggcgaca
    18901 acctggtgct gatgaagcct tcgctcgccg gcctgggcat tgcgcgcctg tgcttcgaga
    18961 ccatccgcga ctacgccgcc gaggccctgc aggccgtcaa ggccaaggcc gtgaccaccg
    19021 cgctggagaa cacggtcgaa gccaccatct acatgagcgg ggtgggcgcc gaatcgggcg
    19081 gtctggccgc cgcccatgcc atccacaacg gcatgaccgc cgtgcacgac ctacatggcg
    19141 cgcagcacgg cgagaaggtc gccttcggcc tggtggcgca actggtgctg gaaggcgcgc
    19201 cgcgcgccga actggagcag gtcatccaca tcatcaagtc ggtggagttg ccgctgacgc
    19261 tggccgacct gggtctgaag aagttcgatg aagccgaatg gcgccgcgcc gccgagctgg
    19321 cctgcgccga gggcgagacc atccacaacg agcccttcaa ggtcacgccc gccatggtct
    19381 acgacgccat tgtcaccgcg gatcggctgc tgcagagcta caaagattga atcgggatag
    19441 gggagccgct atcgtagcgg ctgcccctcc cacaccaccc gg
//
LOCUS       DAOMVO010000013        18774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_543_length_18774_cov_14.341738, whole genome shotgun sequence.
ACCESSION   DAOMVO010000013 DAOMVO010000000
VERSION     DAOMVO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18774
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_543_length_18774_cov_14.341738"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PKV30_01540"
     CDS             complement(<1..1045)
                     /locus_tag="PKV30_01540"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HRL28936.1"
                     /translation="MATLERRAYAEIFGPTVGDRVRLADTDLIIEVEQDHTLRAGGHG
                     EEVKFGGGKTIRDGMAQSQRARAQGAVDTVITNALILDHWGIVKADIGLKDGRIRSIG
                     KAGNPDVQPGVDIVIGPGTEVIAGEGHIVTAGGIDTHIHFICPQQIEEALASGITTLI
                     GGGTGPATGTLATTCTPGPWHIERMLQAADAFPINLGFLGKGNASLPAALHEQIGAGA
                     IGLKLHEDWGSTPAAIDNCLRVAEETDTQVAIHTDTLNESGFVEDTIAAFKGRTIHTF
                     HTEGAGGGHAPDILKVVGEANVLPSSTNPTRPYTVNTLDEHVDMLMVCHHLDASIAED
                     LAFAESRIRKETIA"
     gene            complement(1133..1438)
                     /locus_tag="PKV30_01545"
     CDS             complement(1133..1438)
                     /locus_tag="PKV30_01545"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HRL28937.1"
                     /translation="MTPGELLIDAGAHALNPGRRTTTLVVRNTGDRPIQVGSHYHFAE
                     TNAALDFDRSAARGMRLNIGSGLAVRFEPGQQRTVELVDYAGERVVHGFRGLVRGAL"
     gene            complement(1449..2033)
                     /locus_tag="PKV30_01550"
     CDS             complement(1449..2033)
                     /locus_tag="PKV30_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRL28938.1"
                     /translation="MKNRAFILLGLLWLALPALAHTGALGDHVHTGFMAGLLHPFTGI
                     DHLAAMLAVGVWSALAVRPVWVAPLAFVALLGAGALGGLAGVAVPGMEPMIAASLLVT
                     GLLIATRAQWPVWAAALVAGLFAFFHGAAHGMELSGVGVAQALAGMLIGSAALHLTGI
                     GLGRWVFDQRRWLSAMAGGAVALLGSALLLRMAW"
     gene            complement(2085..2387)
                     /locus_tag="PKV30_01555"
     CDS             complement(2085..2387)
                     /locus_tag="PKV30_01555"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HRL28939.1"
                     /translation="MELTPREKDKLLIFTAALLAERRRARGLKLNYPEAIALISAAVM
                     EGARDGRSVAELMSAGREVLARTDVMDGVPEMIADIQVEATFADGTKLVTVHQPIV"
     gene            2672..3952
                     /gene="urtA"
                     /locus_tag="PKV30_01560"
     CDS             2672..3952
                     /gene="urtA"
                     /locus_tag="PKV30_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HRL28940.1"
                     /translation="MQRRFTLKALAVATALAGVSALPVHAQSGGTIKIGVLHSLSGTM
                     AISETVLKDTVLMAIDEINAQGGVLGKKLEPVVVDPASNWPLFAEKAKQLLTQDKVAA
                     VFGCWTSVSRKSVLPVFEESNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVDYLM
                     SKEGGAAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDSDIDEKYTPFGHSDYQTIVA
                     DIKKFSAGGKTAVVSTINGDSNVPFYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKP
                     LVGHLAAWNYFMSLKNPANSAFTQKWAAYAKAKNIAGHKDKPLTNDPMEATYIGIHMW
                     KQAVEKAKSTDTDKVIAAMAGQTFAAPDGFTVKMDEKNHHLHKPVYVGEIRADGQFNV
                     VWKTPGPIKAKPWSPYIEGNDKKKDEPEGRSKAM"
     gene            4078..5652
                     /gene="urtB"
                     /locus_tag="PKV30_01565"
     CDS             4078..5652
                     /gene="urtB"
                     /locus_tag="PKV30_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HRL28941.1"
                     /translation="MVRLLLACALALGLAGGAHALTPEQARAIAAGDSTDARIAALNQ
                     AVQTADARTAAFVDALANDAVKLAGGQVLVVDGERAHDPVTGAAAALPADAEDVINNN
                     LMRAALEAAQAALQLGSAEEPVRLAAARALLSEPDASRLPLIEAALASEKSPAVRAQL
                     ELVRAATWLASDDKSQRLAAAEALGAQRSPDVKLLLNQRLADEPDAEVKAALQRAIAH
                     IDASLAWGERLGVLFTGLSLGSILLLAALGLAITYGLMGVINMAHGELIMIGAYATYV
                     VQGVFQRHLPESAFGWYLVAAVPVAFLASALVGAVLERGVIRFLYGRPLETLLATFGI
                     SLILQQLVRTIFGAQNVGLENPPWMSGGWQLLPNLTLPWNRIVIVLFAAAVVAGVAWA
                     VARTRLGLFVRGVTQNRPIAACMGVNTARVDTYAFALGSGIAGLAGCALSQVGNVGPD
                     LGQSYIVDSFMVVVLGGVGQLAGTVYAALGLGVLNKLLEGWTGAVLAKIAVLVFIIVF
                     IQKRPQGIFALKGREA"
     gene            5658..6863
                     /gene="urtC"
                     /locus_tag="PKV30_01570"
     CDS             5658..6863
                     /gene="urtC"
                     /locus_tag="PKV30_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HRL28942.1"
                     /translation="MSYQSVNLPRRAPLLSRAGWAAFFTALLAVCALAPALNLWVPEG
                     SPFHLSGYMVTLLGKIMCYAICALAIDLIWGYTGILSLGHGLFFALGGYMMGMYLMRQ
                     IGREGSYQSELPDFMVFLDWKQLPWHWALSDSFVATLILIVAVPGLVAFVFGYFAFRS
                     RIQGVYFSIITQALTFAAMLLFFRNETGFGGNNGFTDFKRILGLPLATPQMRMALFVL
                     TGVALLGFFLLARWLVNAKFGRVLQAIRDAERRTMFCGYDPLPYKLTIWVISAMMCGV
                     AGALYVPQVGIINPSEMSTGASIEMAVWAAVGGRATLIGPIVGAFLVNGAKSWLTVAA
                     PEYWLFFLGGLFIVVTLFMPQGVVGLVGRVLKRNRPPAHVQQAPAATKSEASESTNEA
                     NAALQGARA"
     gene            6860..7744
                     /gene="urtD"
                     /locus_tag="PKV30_01575"
     CDS             6860..7744
                     /gene="urtD"
                     /locus_tag="PKV30_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HRL28943.1"
                     /translation="MTPELMEEGARRLQAHALNAAPRQTESGGRTADYGRPARPGEVD
                     VTHGRILYLDDVSVSFDGFKAINGLNLDIAPGELRCVIGPNGAGKTTMMDIITGKTRP
                     GSGTVFFGSTIDLLRHNEPEIAQLGIGRKFQKPTVFEQLTVFENLELALKTDKRVRPS
                     MLFRLDSAAEDRLAEVLRTIHLAGEVTRRAGDLSHGQKQWLEIGMLLMQDPKLLLLDE
                     PVAGMTDEETARTAELFLSLKGRHSLMVVEHDMHFIRAIAGDTGTVTVLAEGRVLAQG
                     TLDQVQADERVIEVYLGR"
     gene            7816..8508
                     /gene="urtE"
                     /locus_tag="PKV30_01580"
     CDS             7816..8508
                     /gene="urtE"
                     /locus_tag="PKV30_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HRL28944.1"
                     /translation="MLTVKNIQQYYGGSHILRDVGFEARAGEVTVLLGRNGVGKTTLL
                     KSLMGLVPIKSGHIEFDGQRIERRTPYERARAGIGFVPQGREIFSRLTVQDNLAMGLA
                     YRPAGTPIPRELFELFPILEQMRGRRGGDLSGGQQQQLAIARALAPGPRLLVLDEPTE
                     GIQPSIIKDIGRVIRLLAARGDMAIVLCEQYYDFAEELADQYLVMERGEIIARGPGSE
                     MAARGIRRLVAI"
     gene            complement(8524..9354)
                     /locus_tag="PKV30_01585"
     CDS             complement(8524..9354)
                     /locus_tag="PKV30_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HRL28945.1"
                     /translation="MTWHATLDLRYQLAQGRTVVRHRHAGPLRVLKSMHPEDARICHS
                     VLVHPPGGIASGDVLDISVEVESGAHALVTTPGATRFYRSAGAPGVQNVQLRLGEQAR
                     LEWLPLETLIYPGAHAENRLTLELAGGASLIGWDVVALGLPLAGQPFDRGHLLQHIEW
                     PGLWLEHARIDGQDDRLLNSPLGLAGQRCLATLFYASAAPLTRAQREAALERTRAVID
                     AHALAAHAGVTALNDRLLVVRALAPVTEPASDLWRQIRAAWRATFWQLPASAPRIWAM
                     "
     gene            complement(9380..11212)
                     /locus_tag="PKV30_01590"
     CDS             complement(9380..11212)
                     /locus_tag="PKV30_01590"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HRL28946.1"
                     /translation="MSLDLDIGFDSQAGRKRVNEDFAGMQLPGPGEGAHGAIAAIADG
                     VSTGGFGAEAAQTSVTTVVRDYYGTPAAWDSTVALDRVIAAQNAWLAGTNRRRAPASG
                     LTTLTVLVLRGHGWSLAHVGDTRAYLVRGGRVTLLTHDHAVAQHDFRHQLTRAVGAEE
                     RVVVDYTQGDLLVGDLFVLLTDGVHGALSERHLAALLHGSTAATKGVAGAQAFSQALT
                     AAALEAGSPDNATALVVRVRGLQEDTLNDQRRRAATLPVPPRLAVGEAIDGWLVDAVL
                     ADNGINAVYKIRSEEWDQTGAGGQKDPDSAATAPRALVMKALLPARAHDPEERAQLAH
                     EAWLARRLATSAAAPHLVALHEPHEMPGHEPSAFYLLYDWHAGDTLAQVLERGQPPAV
                     SHVLQWAMQAARSLAGLHRQGVVHRDVKPANLHLGADGVLRLLDLGVALSGHEPEAMR
                     RLHAGTPSYINPEQWGYRFGASSGQHAHEAQPADAGSDLFALGVVLYQLLARGKLPYG
                     DVLPYQLGRYWRDPVPPSRHNPEVPIWLDHIVQKAVARDARERFETAEEFMLALERGA
                     SRPLTAPPATPLLQRDPTQWWKLLLGVSLLFNLLLMYWLLALPR"
     gene            11449..13932
                     /gene="nirB"
                     /locus_tag="PKV30_01595"
     CDS             11449..13932
                     /gene="nirB"
                     /locus_tag="PKV30_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HRL28947.1"
                     /translation="MKKPRLVMIGNGMAGVRTLEELIKLAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTLEEIILNDWAWYTDNHVTLHAGCKVTHVDRARRLVHAENAAGDKM
                     VAEYDRLLMATGSHPFILPVAGNDLPGVLAYRDIADTQAMIDAAAKFRHAVVIGGGLL
                     GLEAANGLMKRGMSVTVVHVSPTLLERQLDATAGRLLQKSLEERGLKFLIGAQTQALV
                     AGPDGRVAKVVFKDGTEAPADLVVMAVGIRPNTELAEGMRLHVNRGIVVSDTMQTVTD
                     GRIYAVGECAAHRGIAYGLVAPLFEQGKVVANHLAEFGIGRYQGSLTSTKLKVTGIDL
                     FSAGNFQGGQETEEIVLSDPFAGVYKKLVIQDDKLVGACLYGDTVDGSWYFKLLREGR
                     SVADIRERLMFGESNIGDTGHEGNTKAAAMADTDEVCGCNGVTKGAICKAIKDKGLFT
                     LEEVRKHTKASASCGSCTGLVEQLIMFTAGGDYSASPRLKPLCGCTEHGHQAVRDAIR
                     EHHLLSVPAVMRFMDWTTPDGCPSCRPALNYYLLSTWPKEAVDDPQSRFINERSHANI
                     QKDGSYSVIPRMWGGETNASELRRIADVVDKYAIPTVKVTGGQRIDLLGVKKEDLIGV
                     WQDLGMPCGHAYAKALRTVKTCVGSEWCRMGTQDSTQMGKDLERAMWRMYAPHKVKFA
                     VSGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEVAQFLVKLKTAEEVLEHTGA
                     FMQLYREEGWYLERTVHYIHRVGLDHVRQRVVDDADGRRALWERLQFALDGEPDPWFE
                     FDQARVDTRQFQPVAEVEAAKTAVATPVV"
     gene            14011..14397
                     /gene="nirD"
                     /locus_tag="PKV30_01600"
     CDS             14011..14397
                     /gene="nirD"
                     /locus_tag="PKV30_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HRL28948.1"
                     /translation="MNEWTAICRVQDIPPLGARRVARQRGLDVAIFRTSGDEVFALLD
                     RCPHKGGVLSQGIVFGRQVACPLHNWTIGLDNGAAREPDEGCTPSFAVRVDAGMVYLD
                     ADELATRALEPTRPVAGPCHRHVAPT"
     gene            14431..>15017
                     /locus_tag="PKV30_01605"
     CDS             14431..>15017
                     /locus_tag="PKV30_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRL28949.1"
                     /translation="MPETKSTCPYCGVGCGVIIESDGAAITGVRGDPDHPANFGRLCS
                     KGSTLHLTASPMINATARLLHPMRRPTRGAAPERITWDDALDEVATRLHTVMAQHGPE
                     SIGVYGSGQLLTEDYYVLNKFVKGLLGTNNLDTNSRLCMSSAVAGYKATLGADAPPAC
                     YDDIAHAQCLFITGSNAAWAHPVLFRRIEDARRVNP"
     assembly_gap    15018..15117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15118..17118
                     /locus_tag="PKV30_01610"
     CDS             <15118..17118
                     /locus_tag="PKV30_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRL28950.1"
                     /translation="RVCGVSPEDLLTAARWFAGIGDDGAREPTLSLYCQGLNQSTSGT
                     AKNAALINLHLACGQIGKPGAGPFSLTGQPNAMGGREVGGMANLLSAHRDLANPVHRA
                     EVAALWGVDSVPEKPGLTAVEMFQAAADGQIKALWILCTNPAQSLPDQNTVRRALQRA
                     EFVVVQEAFASSETCAYADLLLPAAPWGEKEGTVTNSERRISRVRAAVAPAGEARADW
                     RIVQAVAQRLETRLRPGRATLFPYQSAEHVWNEHRESTRGRDLDITGLTYRLLDQRGP
                     QQWPFTEADAVGTPRLYVDGVYPTADGRARFHSAPFVPVAEARSAHYPFSLITGRLRD
                     QWHGMTRTGLLGRLFGHASEPVAHMHPHDMARRGFAAGQLVRLRSRHGEIVLPVAENA
                     AVGVSQISVPMHWGSAFLGGQAADGTPLLGINALTTPRFCPTSKQPELKHVAVRVEAV
                     SLPWQLLGLAWLPEARIMATRERLAGMLARFEFANCVPFGRERGGLLFRAAASEPPPD
                     ALLDQIESLLGLNTPDTLRYADRHNAQRRAARLARGQVAEAPERVTLEAILLAGDTQA
                     EAWIRGVLQDQQAAEAYGRLLLAPGARAPVQISQRSPQICTCLNITEAAIVDGLANCD
                     GDDHTRLAQLQGALKCGTQCGSCLPELRRLVSRTHEAMNGPG"
     gene            17304..18566
                     /locus_tag="PKV30_01615"
     CDS             17304..18566
                     /locus_tag="PKV30_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL28951.1"
                     /translation="MTNFRNFLKSGHGPTLFASFLYFDFTFAIWVLNGAMAPFISEQF
                     NLSPAQKGFMVSVPIIAGALMRFPLGVLSQYIGRKSAALVEMGLIVLALLYGFFAVNT
                     YDEVIAMGVLLGIAGGSFGIALSLGSGWFPARYKGLAMGIAGAGNSGTVLAVLFAPPL
                     AMKFGWTAVYGIAACTMIVPMVVLWLMAKEPPDRETGHTLREHTACLFEKDGWAFSLI
                     YVITFGGFIGLASFLPTYFYDQFKVTKVEAGQLTMLATLMGSVIRIVGGYLGDRFGGV
                     NTLTGVLVAVAVTLVGCGMAGTSLVATTLLFMLCFAALGAGNGALFQLVPLRWPLTTA
                     VAGSMIGEIGALGGGFLPNAMGQSKQLAGTYLWGFVAFAALTVGVLVMMRFMQLRWTR
                     TWAEKGGRARAASADLPAVADTSAIARV"
     gene            complement(18694..18770)
                     /locus_tag="PKV30_01620"
     tRNA            complement(18694..18770)
                     /locus_tag="PKV30_01620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18734..18736),aa:Val,seq:gac)
BASE COUNT         3045 a         6357 c         6323 g         2949 t
ORIGIN      
        1 cggcaatcgt ctccttgcgg atgcggcttt cggcaaaggc caggtcttcg gcaatcgatg
       61 cgtcgaggtg gtggcacacc atcagcatgt cgacgtgctc gtcgagcgtg ttgacggtgt
      121 agggacgcgt ggggttggtg gagctgggca gcacgttggc ctcgcccacc accttcagga
      181 tgtcgggcgc gtggccgccg cccgcgcctt cggtgtggaa ggtgtggatg gtgcggccct
      241 tgaaggcggc gatggtgtct tccacaaagc cgctttcgtt cagcgtgtcg gtgtggatgg
      301 cgacttgcgt gtcggtttct tcggccaccc tcaggcagtt gtcgatggcg gcgggcgtgc
      361 tgccccagtc ctcgtgcagc ttcaggccga tggcgccggc gccgatctgc tcgtgcagcg
      421 cggcgggcag gctggcgttg cccttgccca gaaagcccag gttgatcggg aaggcgtcgg
      481 ccgcctgcag catgcgctcg atgtgccacg gccccggcgt gcaggtggtg gccagggtgc
      541 cggtggccgg accggtgccg ccgccgatca gcgtggtgat gccgctggcc agggcttctt
      601 cgatctgctg ggggcagatg aagtggatgt gcgtgtcgat gccgccagcg gtgacgatgt
      661 ggccctcgcc cgcgatcact tcggtgccgg ggccgatgac gatgtcgacc cccggctgca
      721 cgtcggggtt gccggccttg ccgatggaac ggatgcggcc gtccttcaag ccgatgtcgg
      781 ccttgacgat gccccagtgg tcgaggatga gcgcgttggt gatcaccgtg tccaccgcgc
      841 cttgcgcgcg cgcgcgctgg ctttgcgcca tgccgtcgcg gatggtcttg ccgccgccga
      901 acttgacctc ttcgccatgg ccaccggcgc gcagcgtgtg gtcttgctcc acttcgatga
      961 tcaggtcggt gtcggccagg cgcacgcggt cgcccacggt ggggccgaag atttcggcgt
     1021 aggcgcggcg ctcaagcgtg gccatggcaa gccttcacag tcttcgatac ttcgtttttc
     1081 atagctgcca gcgcttgctg cacaagcgct ggggcctgat ttgattcaat attcacagcg
     1141 caccccgcac cagcccgcga aagccgtgca ccacgcgctc gcccgcgtag tccaccagtt
     1201 ccaccgtgcg ctgctggccg ggctcgaagc gcaccgccag gccgctgccg atgttcagcc
     1261 gcatgccgcg cgccgcgctg cggtcgaagt cgagcgccgc gttggtctcg gcaaagtggt
     1321 agtgcgagcc gacctggatc ggccggtcgc ccgtgttgcg caccaccagc gtggtggtgc
     1381 ggcggccggg gttgagcgcg tgcgcgcccg cgtcgatcag cagttcaccg ggcgtcatcg
     1441 cctgcccctc accacgccat gcgcagcagc agcgccgagc ccagcagcgc caccgcgccg
     1501 ccggccatcg cgctcagcca gcgtctctgg tcgaacaccc agcgccccag cccgatgccc
     1561 gtcaggtgca gcgcggccga gccgatcagc atgccggcca gcgcctgcgc cacgcccacg
     1621 ccgctcagtt ccatgccgtg cgccgcgcca tgaaaaaagg caaacagccc cgccaccagc
     1681 gccgccgccc acaccggcca ctgcgcgcgc gtggcgatga gcagaccggt caccagcagc
     1741 gacgcggcaa tcatcggctc catccccggc accgccacac ccgccaagcc gcccagcgcg
     1801 cccgcgccca gcagcgcgac gaaggccagc ggcgccaccc acaccggccg cacggccagc
     1861 gcgctccaca caccgactgc cagcatggcg gccaggtgat cgatgccggt gaacgggtgc
     1921 agcagcccgg ccatgaaacc ggtgtgcacg tggtcgccca gcgcaccggt gtgggccagc
     1981 gcaggcaggg cgagccacag aagacccaga agaatgaatg ctctattttt catagctgtt
     2041 tgcgcagtct ggaaaagcgc cagggccttg gaagttgttg aaagtcaaac gatgggctgg
     2101 tgcacggtga ccagcttggt gccatcggca aaggtcgctt ccacctggat gtcggcaatc
     2161 atctcgggca cgccgtccat cacatcggtg cgcgccagca cctcgcggcc ggcgctcatc
     2221 agctcggcca cgctgcggcc atcgcgcgcg ccttccatca cggcggcgct gatgagggcg
     2281 atggcctcgg ggtagttcag cttcagcccg cgcgcgcggc ggcgttcggc cagcagcgcg
     2341 gcggtgaaga tgagcagctt gtctttttcg cggggcgtga gttccatggt gtcgcgtcaa
     2401 ggtgtcggga ccagtgctcg caattctcgc gccaacttgg cgcagtcctg ccgaccgccg
     2461 tttgccccgt tttggtgctg gcgccgccac ggggcgcgat cgaggtgcac gcgctggcgg
     2521 catggggtgc gtcgcacgtc ctgacgtggt gcgtgacgcc acgctcgcgc ggccgatggt
     2581 gcacgaaaca tgctggcacg ccgtttgcgt tgatgcagcc gaggccaagg tcggccccgc
     2641 aaaccatcga ttcaactgga gtcccaccaa catgcaacgt cgtttcacac tcaaggcact
     2701 ggccgtggcc acggccctcg cgggcgtcag cgccctgccc gttcacgcgc aatccggcgg
     2761 caccatcaag atcggcgtgc tgcacagcct gtcgggcacc atggcgattt cagagacggt
     2821 gctgaaagac accgtgctga tggccatcga cgagatcaac gcccagggcg gcgtgctggg
     2881 caagaagctg gagccggtgg tggtcgaccc ggccagcaac tggccgctgt ttgccgaaaa
     2941 ggccaaacag ttgctgacgc aggacaaggt ggccgccgtg ttcggctgct ggaccagcgt
     3001 gtcgcgcaag tcggtgctgc ccgtgtttga agaaagcaac ggcctcttgt tctacccggt
     3061 tcaatacgag ggcgaagagc tgagcaagaa cgtgttctac accggcgccg cgcccaacca
     3121 gcaggccatt ccggcggtcg actacctgat gagcaaggaa ggcggcgccg ccaagcgctg
     3181 ggtgctgctg ggcaccgact acgtgtaccc gcgcaccacc aacaagatcc tgcgcgccta
     3241 cctgaagagc aagggcgtga aggacagcga catcgacgag aagtacacgc ccttcggcca
     3301 cagcgactac cagaccatcg tggccgacat caagaagttc tcggccggcg gcaagaccgc
     3361 ggtggtgtcc accatcaacg gcgactccaa cgtgccgttc tacaaggaac tgggcaacgc
     3421 cggcctgaag gccaaggacg tgcccgtggt cgccttcagc gtgggcgagg aagaactgcg
     3481 cggtgtcgac accaagcccc tggtcggcca cctggcggcg tggaactact tcatgagcct
     3541 gaagaacccc gccaacagcg cgttcaccca gaagtgggcc gcctacgcca aggccaagaa
     3601 catcgccggc cacaaggaca agccgctcac caacgacccg atggaagcca cctacatcgg
     3661 catccacatg tggaagcagg cggtggaaaa agccaagagc accgacaccg acaaggtcat
     3721 cgccgccatg gccggccaga cctttgccgc gcccgatggc ttcaccgtca agatggacga
     3781 gaaaaaccac cacctgcaca agccggtgta cgtgggcgaa atccgcgccg acggccagtt
     3841 caacgtggtg tggaagacgc ccggccccat caaggccaag ccgtggagcc cgtacatcga
     3901 aggcaacgac aagaagaagg acgagcccga aggccgcagc aaggcgatgt aaggcgcggc
     3961 ggcggggcac cggtggcccc gcctgcgcag tcttgtcgcc atcgctctct ttgtcgccgc
     4021 gcgcgccacc ttcagcggtg cgggcgtgac gcgcccgcac cctcttgctt gcccaccatg
     4081 gtccgacttc ttcttgcctg cgcgctggcc ctggggcttg ccggcggcgc ccacgcactc
     4141 acgcccgagc aggcgcgcgc catcgccgcc ggtgattcga ccgacgcgcg catcgccgcc
     4201 ctcaaccagg ccgtgcagac cgccgatgcg cgcaccgccg ccttcgtcga cgcgctggcc
     4261 aacgacgccg tcaagctggc gggcggacag gtgctggtgg tcgatggcga gcgcgcgcac
     4321 gacccggtca ccggcgccgc cgccgcgctg cccgccgatg cagaggacgt gatcaacaac
     4381 aacctgatgc gcgccgcgct ggaggccgcg caggcggcgc tgcagctggg cagcgccgaa
     4441 gaacccgtgc gcctggccgc cgcccgggcg ctgctgagcg agcccgatgc atcgcgcctg
     4501 ccgctgatcg aagccgcttt ggccagcgag aaatcccccg ccgtgcgcgc gcagcttgaa
     4561 ctggtgcgcg ccgccacctg gctcgccagc gacgacaaaa gccagcgcct ggccgccgcc
     4621 gaggcgctgg gcgcgcagcg aagccccgac gtcaagctgc tgctcaacca gcgcctggcc
     4681 gacgagcccg atgccgaggt gaaggccgcg ctgcagcgcg ccatcgccca catcgatgcc
     4741 agcctggcct ggggcgagcg gctgggcgtg ctgttcaccg ggctcagcct gggctcgatc
     4801 ctgctgctgg cggcgctggg cctggccatc acctacgggc tgatgggcgt catcaacatg
     4861 gcacacggcg agctgatcat gatcggcgcc tacgccacct acgtggtgca aggcgtgttc
     4921 cagcgccatc tgcctgaaag cgctttcggc tggtacctgg tggcggcggt gccggtggcc
     4981 tttctggcct cggcgctggt cggcgcggtg ctggagcgcg gcgtgatccg ctttctctac
     5041 ggccgcccgc tggagacgct gctggccacc ttcggcatca gcctgatcct gcagcagctg
     5101 gtgcgcacca tcttcggcgc gcagaacgtg gggctggaga acccgccctg gatgagcggc
     5161 ggctggcagc tgctgcccaa cctgacgctg ccctggaacc gcatcgtcat cgtcttgttt
     5221 gccgccgccg tggtggccgg cgtggcctgg gcggtggcgc gcacgcgcct gggcctgttc
     5281 gtgcgcggcg tgacgcagaa ccgcccgatt gccgcctgca tgggcgtgaa caccgcgcgc
     5341 gtcgacacct atgccttcgc actcggctcc ggcatcgccg ggctggccgg ctgcgccctg
     5401 agccaggtcg gcaacgtcgg gcccgacctg gggcagagct acatcgtgga cagcttcatg
     5461 gtggtggtgc tgggcggcgt cggccaactg gccggcacgg tctacgccgc actcggcctg
     5521 ggcgtgctga acaagctgct ggaaggctgg accggcgccg tgctggccaa gatcgcggtg
     5581 ctggtcttca tcatcgtctt catccagaag cggccgcagg ggatctttgc cttgaaaggc
     5641 cgcgaggcgt gaccgtcatg agctatcaat cagtcaacct gccgcgccgc gcgccgctgc
     5701 tctcgcgcgc cggctgggcc gccttcttca ccgcgctgct cgccgtgtgc gcgctggcgc
     5761 cggcgctcaa cctgtgggtg cccgaagggt cgccgtttca cctgtcgggc tacatggtca
     5821 cgctgctggg caagatcatg tgctacgcca tctgcgcgct ggccatcgac ctgatctggg
     5881 gctacaccgg catcctgtcg ctcggccacg gtttgttctt tgcgctgggc ggctacatga
     5941 tgggcatgta cctcatgcgc cagatcgggc gcgagggcag ctaccagagc gagctgcccg
     6001 acttcatggt gtttctggac tggaagcaac tgccgtggca ctgggcgcta tccgacagct
     6061 ttgtggccac cctgatcctg atcgtcgccg tgccggggct ggtggccttc gtgttcggct
     6121 acttcgcgtt tcgctcgcgc atccagggcg tgtacttctc gatcatcacg caggcgctca
     6181 cgtttgccgc catgctgctg ttcttccgca acgagacagg ctttggcggc aacaacggct
     6241 tcaccgattt caagcgcatc ctgggcctgc cgctggccac gccgcagatg cgcatggcgc
     6301 tgttcgtgct gaccggtgtg gcgctgctgg ggttttttct gctggcgcgc tggctcgtca
     6361 acgccaagtt cggccgtgtg ctgcaggcca ttcgcgacgc cgagcgccgc accatgttct
     6421 gcggctacga cccgctgccc tacaagctca ccatctgggt catcagcgcc atgatgtgcg
     6481 gcgtggccgg cgcgctgtac gtgccgcagg tcggcatcat caacccgagc gagatgagca
     6541 ccggcgcctc gatcgaaatg gcggtgtggg ccgccgtggg cgggcgcgcc acgctgatcg
     6601 ggccgatcgt cggcgccttt ctggtcaacg gcgccaagag ctggctcacg gtggcggcgc
     6661 cggagtattg gctgtttttt ctgggcggcc tgttcatcgt cgtcacgctg ttcatgccgc
     6721 agggcgtggt cggcctggtc gggcgggttc tcaagcgaaa tcggcctcca gcgcacgttc
     6781 agcaagcgcc agcagctacc aaatccgaag cgtccgaatc gacaaacgag gcgaatgcgg
     6841 ccttgcaggg agcgcgcgca tgacgcccga gttgatggaa gaaggcgcgc ggcggctgca
     6901 agcccatgcg ctgaacgccg caccgcggca gaccgaatcg ggcggccgca ccgccgacta
     6961 cggccgcccg gcgcggccgg gcgaggtcga cgtgacgcac gggcgcatcc tgtacctgga
     7021 cgacgtgagc gtgagcttcg acggcttcaa ggccatcaac ggcctgaacc tggacatcgc
     7081 gccgggcgag ctgcgctgcg tcatcggccc caacggcgcc ggcaagacga cgatgatgga
     7141 catcatcacc ggcaagacgc ggccaggcag cggcaccgtg ttcttcggct ccaccatcga
     7201 cctgctgcgc cacaacgagc ccgagatcgc gcaactgggc atcggccgca agttccagaa
     7261 gcccaccgtg ttcgagcagc tgacggtatt cgaaaacctg gagctggcgc tgaagaccga
     7321 caagcgcgtg cggccgtcga tgctgttccg gctcgactcg gccgctgaag accggctggc
     7381 cgaggtgctg cgcaccatcc acctggctgg cgaagtcacg cgccgcgcgg gcgacctgag
     7441 ccacggccag aagcagtggc tggagatcgg catgctgctg atgcaagacc cgaagctgct
     7501 gctgctggac gaacccgtgg ccggcatgac cgacgaggaa accgcgcgca cggccgagct
     7561 gttcttgtcg ctcaaagggc gccacagcct gatggtggtg gagcacgaca tgcacttcat
     7621 ccgcgccatc gccggcgaca ccggcaccgt caccgtgctg gccgaaggcc gcgtgctggc
     7681 gcagggcacg ctcgaccagg tgcaggccga cgagcgcgtg atcgaggtct acctgggccg
     7741 ttgaataatc tgtttttgat agctgctcgc gcttgatgga caagcgctgg cgccctgttt
     7801 gactcataaa ccaccatgct caccgtcaag aacattcagc agtactacgg cggctcgcac
     7861 atcctgcgcg atgtcggttt cgaggcgcgc gccggcgagg tcaccgtgct gctgggccgc
     7921 aacggcgtgg gcaagacgac gctgctcaaa agcctgatgg gcctggtgcc gatcaagagc
     7981 ggccacatcg agttcgacgg tcagcgcatc gagcgccgca cgccctacga acgcgcgcgc
     8041 gccggcatcg gcttcgtgcc gcaggggcgc gaaatcttct cgcgcctgac ggtgcaggac
     8101 aacttggcca tgggcctggc ctaccggccg gcgggcacgc cgatcccgcg cgagctgttc
     8161 gagctgtttc ccatcctcga gcagatgcgc gggcggcgcg gcggcgatct gtcgggcggg
     8221 cagcagcagc agctggcgat tgcgcgggcg ctggcgcccg gccccaggct gctggtgctg
     8281 gacgagccga ccgagggcat ccagcccagc atcatcaagg acatcggccg cgtcatccgc
     8341 ctgctggccg cgcgcggcga catggccatc gtgctgtgcg agcagtacta cgactttgcc
     8401 gaagagctgg ccgaccagta tctggtgatg gagcgcggcg agatcatcgc gcgcgggccc
     8461 ggcagcgaga tggcggcgcg cggcattcgc cgcctggtgg cgatctgaac tgcagcggcc
     8521 ggctcacatg gcccagatgc gcggcgcgct cgccggcagc tgccagaacg tcgcgcgcca
     8581 ggcggcgcgg atctggcgcc acaggtcgct cgccggctcg gtaaccggcg ccagcgcgcg
     8641 caccaccagc agccggtcgt tcaaggcggt gacgcccgcg tgcgccgcca gcgcatgcgc
     8701 gtcgatgacg gcgcgcgtgc gctccagtgc cgcttcgcgc tgcgcgcgtg tcagcggcgc
     8761 ggcgctggcg tagaacagcg tcgccaggca gcgctggccg gccaggccca gcgggctgtt
     8821 cagcaagcgg tcgtcctggc catcgatgcg cgcgtgctcc agccagaggc cgggccattc
     8881 gatgtgttgc agcaggtggc cgcggtcgaa cggctgcccg gcaagcggca ggccgagcgc
     8941 caccacgtcc cagccgatca gcgacgcacc gcccgccagt tccagcgtca aacggttctc
     9001 ggcgtgggcg ccggggtaga tcagcgtctc cagcggcagc cattcgagcc gtgcctgctc
     9061 gcccaacctg agctgcacgt tctgcacgcc gggcgcgccg gcgctgcggt aaaagcgcgt
     9121 ggcgccgggc gtggtcacca gcgcgtgcgc gccggattcg acttccaccg aaatgtccag
     9181 cacgtcgccc gacgcaatgc cgcctggcgg atgcaccagc acgctgtggc agatgcgtgc
     9241 gtcttccggg tgcatgctct tgagcacccg caacggcccc gcgtgccggt gccgcaccac
     9301 ggtgcggccc tgcgccagct ggtaacgaag atcgagtgtg gcgtgccagg tcatgaaaga
     9361 gggaggtgag cagcccaccc tacctcggca acgccagcag ccagtacatg agcagcaggt
     9421 tgaagagcag gctgacgccg agcagcagtt tccaccattg cgtcgggtcg cgttgcagca
     9481 gcggcgtggc cggcggcgcg gtaagcgggc gcgaggcacc gcgctccagc gccagcatga
     9541 actcttcggc ggtctcgaag cgctcgcgcg catcgcgcgc cacggctttc tgcacgatgt
     9601 ggtcgagcca gatcggcacc tccgggttgt ggcggctggg cggcaccgga tcgcgccagt
     9661 agcgccccag ctggtacggc agcacgtcgc cgtacggcag cttgccgcgc gccagcagct
     9721 ggtacagcac cacgccaagc gcgaacagat cgctgccggc atcggccggc tgggcttcgt
     9781 gcgcgtgctg gcccgacgac gcgccaaagc ggtaacccca ttgctcggga ttgatgtagc
     9841 tgggcgtgcc ggcgtgcagc cggcgcatcg cctcgggctc gtgccccgac agggccacgc
     9901 ccaggtcgag caggcgcagc acgccatcgg cgcccaggtg caggttggcc ggcttgacgt
     9961 cgcggtgcac cacgccctgc cggtgcagac cggccagcga acgcgccgcc tgcatggccc
    10021 actgcaacac gtgcgacacg gccggcggct ggccgcgctc cagcacctgc gccagcgtat
    10081 cgccggcatg ccagtcgtac agcaggtaga aggcgcttgg ctcgtggcct ggcatctcgt
    10141 gcggctcgtg cagcgcgacc aggtgcggcg ccgcggcgct ggtggccagg cggcgcgcca
    10201 gccaggcttc gtgcgccagc tgggcgcgct cctcggggtc gtgcgcgcgg gccggcaaca
    10261 aggccttcat caccagcgca cgcggtgcgg tggcggccga atcggggtct ttctggccgc
    10321 cagcgcccgt ctgatcccat tcttcgctac ggattttata gaccgcgttg atgccgttgt
    10381 cggccagcac cgcatcgacc agccagccgt cgatggcctc gcccaccgcc agccggggcg
    10441 gcaccggcag cgtggcagcg cggcggcgct ggtcgttgag cgtgtcttcc tgcagcccgc
    10501 gcacccgcac caccagggcg gtggcgttgt cggggctgcc ggcttccagc gcggcggccg
    10561 tgagcgcctg gctgaacgcc tgggcccccg ccacgccctt ggtggcggcg gtgctgccgt
    10621 gcagcagcgc ggccaggtgc cgctcgctca aggcgccgtg cacgccgtcg gtgagcagca
    10681 cgaacaggtc gcccaccagc aggtcgccct gggtgtagtc gaccaccacg cgctcttcgg
    10741 cgcccacggc gcgcgtcagc tggtggcgaa agtcgtgctg cgccacggcg tggtcgtgcg
    10801 tgagcagcgt gacgcgcccg ccgcgcacca gataggcgcg cgtgtcgccc acgtgcgcca
    10861 gcgaccagcc gtgcccgcgc agcaccagca ccgtgagcgt ggtcaggccg ctggccggcg
    10921 cgcggcggcg gttggtgccg gccagccagg cgttctgcgc cgcaatgacg cgatccagcg
    10981 caacggtcga atcccacgcc gctggcgtgc cgtagtagtc gcgcaccacg gtggtgacgc
    11041 tggtctgcgc cgcctcggcg ccgaagccgc cggtgctgac gccgtcggca atcgccgcga
    11101 tggccccgtg cgcgccctcg cccggaccgg gcagctgcat gcccgcgaag tcttcgttca
    11161 cgcgcttgcg gccggcttgc gaatcaaagc cgatatcgag gtcgaggctc atgtcgacga
    11221 tgaggaatgc aggttttgtg ccgcgctggt gcatgctttt tggcacaccg ctgcaccgaa
    11281 gcggtgcacg ttcaccaaag gctgaaaaat atcattttga tagctgcccg cgcttgatgg
    11341 acaagcgctg cgcggccttt tgacgtgaaa accaaggcca tcgagagtct ggcacgccgt
    11401 ttgcagcaag acctgcgctg gcgcagggcc cgcagttaga ggtacagcat gaagaaaccc
    11461 agattggtga tgatcggcaa tggcatggcg ggcgtgcgca cgctggaaga gctcatcaag
    11521 ctcgcacccg atctgtacga catcaccgtc ttcggcgccg agccgcaccc caactacaac
    11581 cgcatcctgc tgtcgcccgt gctggcgggc gagcagacgc tggaggagat catcctcaac
    11641 gactgggcct ggtacaccga caaccacgtg acgctgcacg cgggctgcaa ggtcacccac
    11701 gttgaccgcg cgcgccgcct ggtgcacgcc gagaacgccg ccggcgacaa gatggtggcc
    11761 gagtacgacc gcctgctgat ggccaccggc agccaccctt tcatcctgcc ggtggcggga
    11821 aacgacctgc ccggcgtgct ggcctaccgc gacatcgccg acacccaggc catgatcgat
    11881 gccgccgcga aattcaggca cgcggtggtc atcggcggtg gtctgctggg gctggaggcg
    11941 gccaacggcc tgatgaaacg cggcatgagc gtcaccgtgg tgcacgtctc gcccactttg
    12001 ctggagcgcc agctcgacgc caccgccggc cggctgctgc agaaatcgtt ggaagagcgg
    12061 gggctgaagt ttctcatcgg cgcgcagacg caggcgctgg tggccgggcc ggacggccgc
    12121 gtcgccaagg tggtgttcaa ggatggcacc gaagcgcccg ccgacctggt ggtgatggcc
    12181 gtcggcattc gccccaacac cgaactggcc gagggcatgc gcctgcatgt gaaccgcggc
    12241 atcgtggtca gcgacaccat gcagacggtg accgacggcc gcatctacgc cgttggcgaa
    12301 tgcgccgcgc accgcggcat cgcgtacggg ctggtggcgc cgctgttcga gcagggcaag
    12361 gtggtggcca atcacctggc cgagttcggc atcgggcgct accagggctc gctcacgtcc
    12421 accaagctca aggtgacggg gatcgacctg ttctcggccg gcaatttcca gggcgggcag
    12481 gagacggagg aaatcgtgct ctccgacccg tttgccggcg tctacaagaa gctggtcatc
    12541 caggacgaca agctggtcgg cgcctgcctg tacggcgaca cggtcgatgg ttcgtggtac
    12601 ttcaaactgc tgcgcgaagg ccgcagcgtg gccgacatcc gcgagcggct gatgttcggc
    12661 gagtcgaaca tcggcgacac cggccacgaa ggcaatacca aggccgccgc catggccgac
    12721 accgacgaag tctgcggctg caacggcgtg accaagggcg ccatctgcaa ggccatcaag
    12781 gacaagggcc tgttcacgct ggaggaagtg cgcaagcaca ccaaggccag cgcctcgtgc
    12841 ggctcgtgca cgggcctggt ggagcagctc atcatgttca ccgccggcgg cgactattcg
    12901 gccagcccgc ggctcaagcc cctgtgcggc tgcaccgagc acggccacca ggccgtgcgc
    12961 gatgcgattc gcgagcacca cctgctcagc gtgccggcgg tgatgcgctt catggactgg
    13021 acgacgcccg atggctgccc cagctgccgc ccggcgctca attactacct gctgtccacc
    13081 tggccgaagg aggcggtgga cgatccgcaa agccgcttca tcaacgagcg cagtcacgcc
    13141 aacatccaga aggacggcag ctacagcgtc atcccgcgca tgtggggcgg cgagaccaat
    13201 gccagcgagc tgcgccgcat tgccgacgtg gtggacaagt acgccattcc cactgtcaag
    13261 gtcacgggcg gtcagcgcat cgacctgctg ggcgtgaaga aggaagacct gatcggcgtc
    13321 tggcaagacc tgggcatgcc ctgcggccac gcctacgcca aggcgctgcg caccgtgaag
    13381 acctgcgtgg gcagcgagtg gtgccgcatg ggcacgcagg acagcacgca aatgggcaag
    13441 gatctggagc gcgccatgtg gcgcatgtac gcgccgcaca aggtcaagtt tgccgtcagc
    13501 ggctgcccgc gcaactgcgc ggagagtggc atcaaggacg tgggcgtgat aggcgtcgat
    13561 tcgggctggg agatctacgt cggcggcaac ggcggcatca agaccgaggt ggcgcagttt
    13621 ctcgtcaagc tgaagaccgc cgaggaagtg ctggaacaca ccggcgcctt catgcagctg
    13681 taccgcgagg agggctggta cctggagcgc accgtgcact acatccaccg cgtcgggctc
    13741 gaccacgtca ggcaacgcgt ggtggacgat gccgatggcc gccgtgcatt gtgggagcgg
    13801 ctgcagttcg cgctcgacgg cgagcccgac ccctggttcg agttcgacca ggcgcgggtc
    13861 gacacgcggc agtttcagcc agtggcagaa gtcgaggccg cgaagacggc cgtggccacg
    13921 ccagtggtct agctccagcc ccggaaatat cgatcaaatg tgccgctggc gcttgaatac
    13981 attgcgcgag cagctatcaa ggatgtagca atgaacgagt ggactgcgat ctgccgggtg
    14041 caggatattc cgcccttggg cgcgcgccgc gtggccaggc agcgcggcct cgacgtggcg
    14101 atctttcgca ccagcgggga cgaggtgttt gccctgctcg accgctgccc gcacaagggc
    14161 ggtgtgctga gccagggcat cgtgttcggc cgccaggtgg cctgcccgct gcacaactgg
    14221 accatcgggc tggacaacgg cgcggcgcgc gagccggacg aaggctgcac gccgagcttc
    14281 gccgtgcggg tggacgccgg catggtctac ctcgacgccg acgagctggc cacgcgcgcc
    14341 ctcgagccca cgcggccggt ggccgggccg tgccaccggc acgtggcgcc cacgtgacgg
    14401 ctgatcgcgt ccgtagctga acacgccgtg atgcccgaga ccaaatccac ctgcccttat
    14461 tgcggcgtgg gctgcggcgt gatcatcgaa tccgacggcg ccgcgatcac cggcgtgcgg
    14521 ggcgaccccg accaccccgc caacttcggc cgcctgtgca gcaagggcag cacgctgcac
    14581 ctgaccgcat cgcccatgat caacgccacc gcccggctgt tgcaccccat gcgccgcccg
    14641 acacgcggcg cggcgcccga gcgcatcacc tgggacgacg cgctggacga agtcgccacg
    14701 cgcctgcaca ccgtcatggc gcagcacggc cccgaatcga ttggcgtgta cggcagcggc
    14761 cagctgctga ccgaggacta ctacgtgctc aacaagttcg tgaaagggct gctgggcacc
    14821 aacaacctcg acaccaattc acgcctgtgc atgagcagcg ccgtggcggg ctacaaggcc
    14881 acgctgggcg ccgacgcgcc gccggcgtgc tacgacgaca tcgcgcacgc gcagtgcctg
    14941 ttcatcaccg gcagcaacgc cgcctgggca cacccggtgc tgtttcgccg catcgaagac
    15001 gcccgacgag tcaacccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
    15121 gtgtgcggcg tgtcgccaga agacttgctg accgccgcgc gctggttcgc gggcatcggc
    15181 gacgacggcg ccagagagcc cacgctcagc ctctactgcc agggcttgaa ccaatcgacc
    15241 agcggcaccg ccaagaacgc cgcgctcatc aacctgcacc tggcctgcgg ccagatcggc
    15301 aagccgggcg ccgggccgtt cagcctgacg gggcaaccca acgccatggg cgggcgtgag
    15361 gtgggcggca tggcgaacct gctcagcgcg caccgcgatt tggccaaccc ggtgcaccgt
    15421 gccgaggtgg ccgccttgtg gggcgtggat tcggtgcctg aaaagcccgg gctcaccgcg
    15481 gtggaaatgt tccaggccgc ggccgatggc cagatcaagg cgctgtggat tctttgcacc
    15541 aaccccgcgc aaagcctgcc cgaccagaat acggtgcgcc gcgccttgca gcgcgccgag
    15601 ttcgtggtgg tgcaggaagc ctttgccagc agcgaaacct gcgcgtacgc cgacctgctg
    15661 ttgcccgccg cgccctgggg cgagaaagaa ggcacggtga ccaacagcga acgccgcatc
    15721 agccgtgtgc gcgccgccgt ggcaccagcg ggcgaggcgc gcgccgactg gcgcatcgtg
    15781 caagccgtcg cgcagcggct ggagacgcgc ctgcggcccg gcagggcgac cttgtttccg
    15841 taccaatcag ccgagcacgt gtggaacgag caccgcgaga gcacgcgggg acgcgatctt
    15901 gacatcaccg ggctgaccta ccggctgctg gatcagcgcg ggccgcagca atggcccttc
    15961 accgaagcgg acgccgtagg aacaccccgc ctgtacgtag acggcgtgta tcccaccgcc
    16021 gatggccgcg cgcgctttca cagcgcccct ttcgtgccgg tggccgaggc gcgcagcgcg
    16081 cactacccct tcagcctgat caccggccgg ctgcgcgacc agtggcacgg catgacgcgc
    16141 accggcctgc tgggccgcct gttcggccat gcgtccgagc cggtggcgca catgcaccca
    16201 cacgacatgg cacggcgcgg cttcgccgcc ggccaactcg tgcgcctgcg cagccggcat
    16261 ggcgaaatcg tgctgcccgt ggcggaaaac gccgccgtgg gtgtcagcca gatcagcgtg
    16321 cccatgcact ggggcagcgc gtttttgggc ggtcaagcgg cggacggcac gccgctgctg
    16381 ggcatcaacg ccttgaccac gccccggttc tgccccacgt cgaagcagcc ggaactcaag
    16441 cacgtcgcgg tgcgggtcga ggccgtctcg ttgccctggc agttgctcgg actggcgtgg
    16501 ctgccggaag cgcgaatcat ggccacgcgc gagcgactgg ccggcatgct ggcgcgtttt
    16561 gaatttgcca attgcgtgcc gtttggccgt gagcgcggtg gcttgctgtt tcgcgcggca
    16621 gccagcgaac caccccccga cgccttgctt gatcagatcg aatcgctgct tggactgaac
    16681 acccccgaca cgctgcgcta tgccgaccga cacaacgccc agcggcgcgc ggcacgtctg
    16741 gcgcgcggcc aggtcgctga agcaccggag cgcgtcacgc tggaggcgat cttgctggcc
    16801 ggtgacacgc aggccgaggc ctggatccgg ggcgtgctgc aagaccagca agccgccgaa
    16861 gcctatggcc gtctgctgct ggcaccaggc gcgcgcgcgc ctgtgcaaat ttcgcagcgt
    16921 tcgccgcaga tttgcacctg cctcaacatc accgaagcag cgatcgtcga tggcctggcg
    16981 aactgcgatg gtgatgacca cacgcgactc gcgcaactgc agggcgcgct gaagtgcgga
    17041 acgcaatgcg gctcttgctt gccggaattg cggcgcctgg tgtcgcgcac ccatgaagca
    17101 atgaacggac cgggttgatc ggcggcatcg cactcgacag gtgccctcgc cccaacaacg
    17161 tgagccttgc cctttgcaag cgccttggaa caacgcgctg catgccgctc ttggtgcatc
    17221 ggatgacttc ttgacgggct tgagcgctgg catccgtctt gcagtggttc ctacgtcgca
    17281 acattcaaag accaagccgc gccatgacaa atttccgaaa cttcctcaag tccgggcacg
    17341 ggccgacgct gttcgcgtcg ttcctctact tcgatttcac ctttgccatc tgggtgctga
    17401 acggcgccat ggcgcccttc atcagcgagc agttcaacct gagcccggcg caaaagggct
    17461 tcatggtcag cgtgccgatc atcgctggtg cgctgatgcg ctttccgctc ggcgtgctgt
    17521 cgcaatacat cgggcgcaag agcgcagcct tggtcgagat gggcctgatc gtgctggcgc
    17581 tgctctatgg ctttttcgcg gtcaacacct atgacgaagt gatcgccatg ggggtgctgc
    17641 tgggcatcgc cggtggcagc ttcggcatcg cactgtcgct gggctccggc tggttcccgg
    17701 ccaggtacaa ggggctggcc atgggcatcg cgggcgccgg caactcgggc acggtgctgg
    17761 cggtgctgtt cgcgccgccg ctggccatga agttcggttg gaccgccgtg tacggcatcg
    17821 ccgcctgcac catgatcgtg ccgatggtcg tgctttggct catggccaag gaaccgcctg
    17881 accgcgaaac cggccacacc ctgcgcgagc acacggcctg cctgttcgag aaagacggct
    17941 gggccttcag cctgatctac gtcatcacct tcggcggctt cattggcctg gcgagttttc
    18001 tgccgaccta tttctacgac cagttcaaag tcaccaaggt cgaagccggg caactgacca
    18061 tgctggccac gctgatgggc tcggtgattc gaatcgtcgg cggctacctc ggcgaccggt
    18121 tcggcggcgt gaacaccctc accggcgtgc tggtggcggt ggccgtgacg ctcgtcggct
    18181 gcggcatggc cggcacgtcg ctggtcgcca caaccttgct cttcatgctg tgcttcgccg
    18241 ccctgggcgc aggcaatggc gcgctgtttc agctggtccc gctgcgctgg ccgctgacca
    18301 ccgcagtcgc cggctccatg attggcgaga tcggtgcgct gggcggtggc tttctaccca
    18361 acgccatggg ccaatccaaa caactcgccg gcacctacct ctggggcttc gtcgccttcg
    18421 ccgccctgac ggtgggtgta ttggtcatga tgcgattcat gcaattgcgc tggacccgga
    18481 cttgggccga aaaaggcggc cgggcgcgag cagcatccgc cgatttgccg gccgttgcag
    18541 atacaagcgc catcgctcgc gtctgatctg ccgacaatcg gcaaggcgcg aaagtcctgg
    18601 ccggcacgca aggccggtca gcgggcgtcc caactcggag cagaaaatcc aagaaaaaag
    18661 cacctagcgt tgccgctaag tgcttgattt cattggtagg cgcgatagga ctcgaaccta
    18721 cgacccccac catgtcaagg tggtgctcta accagctgag ctacgcgcct gaaa
//
LOCUS       DAOMVO010000014        18767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_544_length_18767_cov_11.865701, whole genome shotgun sequence.
ACCESSION   DAOMVO010000014 DAOMVO010000000
VERSION     DAOMVO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18767
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_544_length_18767_cov_11.865701"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="nusA"
                     /locus_tag="PKV30_01625"
     CDS             complement(<1..761)
                     /gene="nusA"
                     /locus_tag="PKV30_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRL28952.1"
                     /translation="MNRELLMLVEAISREKNVERDVVFGAVEAALAQAAKKLYEGEVD
                     LRVGIDRDTGEYETFRRWHVVPDEAGLQLPDQEILLFEAKEQIPDIELDEYIEEQVES
                     VPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGDKIFTGTVKRMDKGDILVESGRV
                     EGRLRRSEMIPKENLRNGDRVRAMIMEVDLTLRGAPILLSRSAPEFMIELFRQEVPEI
                     EQGLLEIKSAARDPGSRAKIAVLSHDKRVDPIGTCV"
     gene            complement(822..1397)
                     /gene="rimP"
                     /locus_tag="PKV30_01630"
     CDS             complement(822..1397)
                     /gene="rimP"
                     /locus_tag="PKV30_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRL28953.1"
                     /translation="MALQQTIDDTIRGLGFELVELERSAGGLLRVTIDFPWSPDEAVR
                     LITVEDCERATRQLQYVLEVEGVDYHRLEVSSPGIDRPLRHAQDFERFEGELVDVTLK
                     APIGEAAAGQVHASRKKFRGTLHKVDDSHWQIVWREEPAAKPGQRVSAKRAPAPEQAL
                     TFLLDELQSARLAPVVDFKGRKSPPPNAKEV"
     gene            1618..1902
                     /locus_tag="PKV30_01635"
     CDS             1618..1902
                     /locus_tag="PKV30_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRL28954.1"
                     /translation="MNNSPASSDVSALLPEVAQLMVTALNLETAPEEIQPDAPLYGGD
                     LGLDSIDILEVALAVSKKYGFQLRADDEDNAAIYASLRSLTQHVAAHRTT"
     gene            1952..3307
                     /locus_tag="PKV30_01640"
     CDS             1952..3307
                     /locus_tag="PKV30_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL28955.1"
                     /translation="MQAQYPLSSHAPDDILAYRHGRGVAAGRFFQDALSLASTLVPGG
                     HVINVCHDRYLFAVGLAAGLLAGKVTLMPPSFAPQVIAQMQTRYPDLRCLHDDRERPE
                     GLAGISVQDHLGGNTSPSNASPLIDAGQIAAIVFTSGSTGEPTAHQKTWGRLCINGAA
                     ESARLGAAGVTIVATVPAQHMYGFESSVLMALHGGASFWHGRPFYPADIVEALASVPR
                     PRMLVSTPFHLSTLFEAGLKLPACDLVLSATAPLSAELAERAEAVMGAPLLEIYGCTE
                     SGQVASRRPVNGPAWQLLPGVQMATDAEGAWVFDGHVEGRVLLSDRIEEQDTEHFLLL
                     GRNADMVNVAGKRTSLAHLNAQLAGIPGVVEGCFIQPDDEAVARDGTVQRLAALVVAP
                     TLGPSEVLAALRQRIDPVFLPRPLLRVDAMPRNATGKLIRSDIMALYRSAKRDSPPEI
                     A"
     gene            3304..3639
                     /locus_tag="PKV30_01645"
     CDS             3304..3639
                     /locus_tag="PKV30_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28956.1"
                     /translation="MTTQEYLQWPLLATWRVPDEHPVLPGHFPAYPVLPGALLLDWVI
                     GLVSSYPGDAAIQVRAARFPAAARAHMLLRAHGLREGGRVQFAVMTDDGSPLCTGTVD
                     GVNKGSLSS"
     gene            3636..4550
                     /locus_tag="PKV30_01650"
     CDS             3636..4550
                     /locus_tag="PKV30_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRL28957.1"
                     /translation="MTPPTQVASAEWATRPERSSMAVLRLMVWLSLFLGRRLSRLVLY
                     PIAAYFVVFAPKAKRASKAYLSRALGRTARLSDGCRHVLTFASVVHDRVYWLRGRHDL
                     FEIEVIGESHLAALRQQGRGMVFAGGHFGSFEALRAVGERHGIDTRMLMFPDNARKVT
                     AALTSINPALANSVIGLGRPDSVLRVRDHLQNGGSVGILADRMLGEDKSRDIPFLGEP
                     APFPVGPWRLAAMLAAPVVFMAGCYLGGNRYQLVFEPIIDFAQVPAEKRASAMQEAQD
                     RFVELLESRCRQAPWNWFNFYDFWHAQT"
     gene            4594..5130
                     /locus_tag="PKV30_01655"
     CDS             4594..5130
                     /locus_tag="PKV30_01655"
                     /inference="COORDINATES: protein motif:HMM:NF039988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRL28958.1"
                     /translation="MLLITSASAWAEWNVASLMQQLARHPAGQARFTETKKLSVLDAP
                     VVSSGRLIYSPPDRLEKITVQPKAESLTVESDQVVVVRDGRRREMRLQQVPEALAIVE
                     GVRGTLLGNEAMLRQYYDLRLVGTEKDWRLALIPREERLARWVRQITVNGQRHVVSSI
                     ETLQADGDSSIMRIEAEK"
     gene            5156..7570
                     /locus_tag="PKV30_01660"
     CDS             5156..7570
                     /locus_tag="PKV30_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRL28959.1"
                     /translation="MLTSSSRTDPPRSHTTRFVLGWWLVLMALCVAVVATSRYSTDMS
                     AFLPREPDARQRLLVDQIKDGALSRMVLLGIDGGTPAARAEASAKLAATLRKSTEFSG
                     VVNGDAASREVDQALLLRYRYVLSPKVNAERFSSQGLHDAISHTLVELSGSAGLFLKS
                     LLPRDPTGELWATLESVAGGNAPSTSDGAWASADGQRALLVTMTRAKGTDTDAQERAL
                     TVIRKSFDALNTDGTLQLRMSGTPVFAVHARSTIRSEIERLSLIGGLSVFALLFGVYR
                     SWRNVAIGLLPVVSGILVAIAAVALSFDTVHAITIGFGTTLIGEAIDYSIYYLVQAHD
                     PQAWRTTYWPTIRLGVATSLCGFAALLFSSFPGLVQLGAYSVAGLIAAAMVTRFVLPA
                     LTTAPVPFARIDRLGRAVAWLAAWMQRLRWLAVGLTLAALVLVLVQRDHLWASGLSGL
                     NPAPLDMQKLDAELRRDAGTPDLQHMVVVTAETEEAALRAAEQAEQSLQPLLASGRLS
                     GIDSPAHFLPSQIVQHERLAALPAADELRSRLTSALKDLPLKIDRLTPFVEDVARAKN
                     EGPLTASALAGSSFAFAVQTLLLPRDGQWSVLMPLRLPAAGASTNTEDLKSAVTTLLN
                     SSPPRVEAFYVNLDEQATSVFGQYLRQALMLALAGSLAVLLLVAVALRDFRRTIRVLA
                     PLVGAVALVMAAHLALGVRMTLLHLVGLLLIVAVGSNYALFFNQRVSQTSGAALPRTT
                     ALASLALANVSTIIGFGVLALSNVPVLNAIGATVGPGALLALLLAMSWAAGDARAASP
                     PGAEAA"
     gene            7669..8253
                     /locus_tag="PKV30_01665"
     CDS             7669..8253
                     /locus_tag="PKV30_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL28960.1"
                     /translation="MFLILFFRAYLAIQRNPVFEVMQIPATAIDRWVGFQPWTLGLYL
                     SLWVYTALPVALQPDLRQLVRYCAHISAMCLLALAVFFLWPTSVSDAVGPRGGGGLFA
                     AMYAVDDAGNACPSLHVAASVFSFLWLRAMLADVGAPRWLALINACWCVGICYSTLAT
                     KQHLLIDVLAGAALGGVGGWWSLRASGRSKQRVQ"
     gene            8301..9656
                     /locus_tag="PKV30_01670"
     CDS             8301..9656
                     /locus_tag="PKV30_01670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL28961.1"
                     /translation="MQSSLPTDQAGDSPVECDVLVIGGGPAGSTVAALLAQKGRHVVL
                     LDKDRHPRFHIGESLLPANLPLFERLGVADEMRAIGIKKRAAEFMSPWHEHKQQFLFA
                     DAWDKTMPHAYQVRRSEFDQVLLRRATQLGAHVREGCKVTAVDLEATPERARAQARCD
                     DGRTLAYAPRFVVDASGRDTFLASRFQIKHKNPQHNSVAVYGHFENVRRNEGEDEGNI
                     IIFWFEHGWFWFIPLQDGVTSIGMVTWPYFMKTRGERSLQQFLLDGIRQCPPLLERMA
                     GARLTDVIEATGNYSYSCERNHGRNYVLLGDAYAFIDPVFSSGVMMAMQGSFDAADAI
                     DSWLDHGAEPALQAAALRKLDARLRHGPRTFSWLIYRMTNPVMRDLFMGPRNPFRVKE
                     ALLSLLAGDIYGRTPIWTSLRIFKLIYYTSSLVQLPTALRAWRRRQTNLRPVDEAAGA
                     R"
     gene            9695..10462
                     /locus_tag="PKV30_01675"
     CDS             9695..10462
                     /locus_tag="PKV30_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL28962.1"
                     /translation="MLKLLWRAYEHVAMVVGLLLLATVCLTWTPFALLLNPLLPEARA
                     KRVGRGAIRGAFRLYLRLLSLLCACRFDLRELQALGRRGEPLVVVANHPSLLDAVILV
                     ACLPNAVCVMKARLMHNVLLGAGARMARYIVNDAPLPMIRGAIAELRQGACLIIFPEG
                     TRTVTPPVDACASTAGVIAARAGVPVQALLIEMSTPYLGKHWPLWRPPVLPLSCRVRV
                     GQRWAVLERPQQFGPEIEAYFREAIDPAANQLPDARE"
     gene            10459..11223
                     /locus_tag="PKV30_01680"
     CDS             10459..11223
                     /locus_tag="PKV30_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL28963.1"
                     /translation="MSASPASTTHLVLIPSYNPGAKVRDTVAAARAVWAPVWVVVDGS
                     TDGSADWLREMAARDSGLRVLVQPENRGKGAAVLAGIEQAAREGFTHALTMDSDGQHP
                     AELIPEFMCASQAAPAAMVLGVPVFGAEAPRIRVLGRKVSNGWANLETLGAGIGDSLY
                     GFRVYPIAPLVRVMRGQRFMRRFDFDPEAAVRLAWAGVHPVNLPAPVRYFTPEEGGVS
                     HFKYLRDNTLLTWMHTRLFIGFLLRLPVLLARRIFR"
     gene            complement(11278..12582)
                     /locus_tag="PKV30_01685"
     CDS             complement(11278..12582)
                     /locus_tag="PKV30_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRL28964.1"
                     /translation="MSVEAAAQEIGVHKAEAVLFFTLLQLTVVVLAGRIGSSLAVRFG
                     QSTAVGEIIVGILLGPSLFGLLAPGTFNYVFHSAPGEPMQVLSQIGLIFLMFQIGLEF
                     DFGHLTERRNRRAVMWVAAAGLLLPFALGLALGWVVAPFLGGRNGQALASALFVATSF
                     SITALPILGRILIEFDMTQRPLGVIAISAAAINDVVGWLLLALVTALSVSQFQAGGFA
                     LNVGAVGLFAVLSWWIARPLLKRLVRWAQNQPARDGAPKALSNNLLGLLIAAIFIGAM
                     TTYKLGIFAIFGGFMLGVLLHDERELVDTWRDRMGQFVTVFFLPIFFTYTGLRTNIGG
                     LDTPALWGWCAVVIGLATVGKFCGSYLAARFVGLPKAESKVLGILMNTRALMELIVIN
                     VGYDLGVISQNLFTMLVLMAIISTVITSPLLRRWLPRCAPGS"
     gene            complement(12579..13319)
                     /gene="fabG"
                     /locus_tag="PKV30_01690"
     CDS             complement(12579..13319)
                     /gene="fabG"
                     /locus_tag="PKV30_01690"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRL28965.1"
                     /translation="MNHSSPTRRALVTGASGAIGGAIARALAAQGLHVLAHANRNRAA
                     ADEVVAAIHATGGAAESVVFDVTDGPACSKALAEMLEAGPIQVLVHNAGVHDDGVFAG
                     LRPEQWQRVIDVNLNGFFNVAQPLMLPMLRTRWGRIVAITSVAGQAGNRGQVNYAAAK
                     SALHGAVKSLALECASRGVTVNAVAPGIVASEMSAGTFDAAAIQRMVPMGRAGQPQEV
                     ADLVAFLSSDKAAYISGQTIGINGAMYQ"
     gene            complement(13316..13795)
                     /locus_tag="PKV30_01695"
     CDS             complement(13316..13795)
                     /locus_tag="PKV30_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxylacyl-ACP dehydratase"
                     /protein_id="HRL28966.1"
                     /translation="MTSPWWAQASGQGHDWIAARVPHQGSMCLLGRVLGASPEGIRCA
                     ADSHHAPDHPMRQHGRLGGACGVEYAAQAMALHGALLAEQRGVPRPRRGLLVSVRGVT
                     LHVPRLDDVAGEITVAARCEAENGDLCQYAFSLHAGDQLLVEGRATVMLDVPVPVSP"
     gene            complement(13804..14625)
                     /locus_tag="PKV30_01700"
     CDS             complement(13804..14625)
                     /locus_tag="PKV30_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HRL28967.1"
                     /translation="MSAREPITAWVDGIGLLAPGIGDWATGRALLRGDAPYTAAPTAL
                     PAPEVLPAAERRRASRIVKAALAVGLEACRAAGADPATLANVFASSGGDGHNGHELCE
                     LLAGDDRQISPTRFHNSVHNAASGYWSIATGATPAAQVLGAYDASVAAGLLEAVTQVH
                     ASGAPVLLVAADSEYPEPLHAKRPIQDTSAFALLLAPAQGPRSLGRLLLPRQGALRDT
                     AGDLTHLPSTLAALVDAMPPMRALPLLAALAGGAARHVALPYWPPQWLWLDVAPA"
     gene            complement(14622..15809)
                     /locus_tag="PKV30_01705"
     CDS             complement(14622..15809)
                     /locus_tag="PKV30_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRL28968.1"
                     /translation="MSLALTAYTASSAVGHGRAALLDALRAGRSGLKACNFETAALAT
                     WMGEVPGVDEVALPPGLAAYDCRNNRLALLGLRADGFEDAARAAIARHGADRVALILG
                     TSTSGMLSAELAYRERDAGGALPAWFDYARTHNTGSLVAFVRELLGVRGPAFAISTAC
                     SSSAKAFAAADRLIAAGLADAAVVGGVDSLCLTTLYGFASLELTSPEPCRPGDAARQG
                     LSIGEAAAFALLEPGDAAVVLSGWGEASDAHHMSAPHPEGRGSRAAMQGALARAGLAP
                     GDIDYINLHGTATPANDVAEGLAVSGVFGTALGGGVPCSSTKGITGHTLGAAGALEAV
                     ACALALEHGLLPPSVGTRTPDPAIPVDLIMLPRTASLRHVLSNSFGFGGSNASLVLSR
                     RGA"
     gene            complement(15813..16634)
                     /locus_tag="PKV30_01710"
     CDS             complement(15813..16634)
                     /locus_tag="PKV30_01710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL28969.1"
                     /translation="MTLQAEPSAEQRLAPTRAEAPPLVAAGAARPDPRALQLALVDAA
                     SARYRAGGRFAWHFARGKLGMDPLFIDLLRLGLLPAHGRFLDLGCGQAVLAAWLLAAE
                     EHHATGQWPAGWGPPPRVQSLHALELMPRDVARGQRAFDGEPRVRVEQGDICQAVFEP
                     VDVITIFDVLHYFPPEAQDDVLRRMRAALGAGSLLLARVGDAAAGWRFELSRRVDQAV
                     TLVRGHGLSPMYCRTAADWAEALRALGFSVQVVRTQGGPPFANTMLVARVPPEGC"
     gene            complement(16631..17488)
                     /locus_tag="PKV30_01715"
     CDS             complement(16631..17488)
                     /locus_tag="PKV30_01715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRL28970.1"
                     /translation="MSVPERTPARWRPSAFLMLSAAVHLAAGIGLLLAPAQWAWWLGA
                     LIVNHGLITAAGLLPRCNWLGPNLTRLPPAAAARGEVALTFDDGPDPAITPQVLDMLD
                     AAGVRASFFCIGERVAAHAALAREIVRRGHHIENHTQHHPHALSLYGPRRLAREVLDA
                     QRTLAQASGRAPRFVRAVAGLRNPFLEPILARHGLQLASWTRRGYDTRNADPAQVLAR
                     LTRGLAAGDILLLHDGHGATTAAGQPVVLAVLPPLLAALRARGLKPVTLDAACAPAPV
                     PAPRQGVPA"
     gene            complement(17608..>18767)
                     /locus_tag="PKV30_01720"
     CDS             complement(17608..>18767)
                     /locus_tag="PKV30_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL28971.1"
                     /translation="HQLSGGQRQRAMIAMALACEPKLLLADEPTTALDVSLRGQILDL
                     LSELQRANGMAVLLITHDLNLVRRFADRVAVMQHGQLVEQGATADIFSAPQHAYTQKL
                     MASKPERDVVETPAAPGDTPALAAHELRVVYPTPLPGIRGWFKKGEFVALQGADLRLL
                     PGRTLGVIGESGSGKSTLAQAALGLLPRAQVGGSVLVAGRPWQLKAAADKPLRRVAQV
                     VFQDPFSSLSPRLTVEEIVGEGLLVHAPELPAPERATRVRAALAEVGLTETQFPNLLA
                     RYPHEFSGGQRQRIALARALIVGPELLVLDEPTSALDVTIQKQVLALLQKLQKERALS
                     YLLITHDVDVVRALAHDVIVLKDGAVVEAGSAAQVLGHAQHPYTRKLLAAA"
BASE COUNT         2986 a         6356 c         6302 g         3123 t
ORIGIN      
        1 acacaggtgc cgatggggtc aacgcgcttg tcgtggctca gcacggcgat cttggcgcgg
       61 ctgccggggt cgcgcgcggc gctcttgatc tcgagcaggc cttgctcgat ctcgggcact
      121 tcctggcgga acagctcgat catgaactcg ggcgccgagc gcgacagcag gatgggcgcg
      181 ccgcgcagcg tgagatcgac ctccatgatc atggcccgca cgcggtcgcc gttgcgcagg
      241 ttctccttcg gaatcatctc gctgcggcgc aggcgccctt cgacccggcc ggattcgacc
      301 aggatgtcgc ccttgtccat gcgcttgacg gtgccggtga aaatcttgtc gccacggctc
      361 atgaagtcgt tcagcagcat ctcgcgctcg gcgtcgcgga ttttctgcag gatgacctgc
      421 ttggccgcca tggcgccgat gcggccgatg ggcaccgatt caacctgctc ctcgatgtat
      481 tcgtcgagtt cgatgtcagg gatctgctcc ttggcttcga acagcaggat ttcctgatcg
      541 ggcaattgca ggcccgcttc gtcgggcacc acatgccagc ggcggaaggt ctcgtattcg
      601 cccgtatcgc ggtcgatgcc cacgcgcagg tcaacctcgc cctcatacag cttcttggcg
      661 gcctgcgcca gcgccgcctc gacggcaccg aacaccacgt cccgctcgac gttcttctcg
      721 cgcgagatcg cttcgaccag catcaacagt tcgcgattca tgatttcacc agctccaatg
      781 ctctcaaaat gaaagcgcgt cgcgcgcgct ccacctattg ttcaaacctc ttttgcatta
      841 ggtgggggcg acttgcgccc tttgaagtcc accacgggcg cgagccgcgc cgattgcaat
      901 tcatccagca aaaacgtcaa cgcctgctcg ggcgcgggcg cgcgcttggc gctcactcgc
      961 tgaccgggct tggccgcggg ctcctcgcgc cacacgatct gccaatggct gtcgtccacc
     1021 ttgtgcaacg tgccgcgaaa cttcttgcga ctggcgtgca cctggcccgc ggcggcctcg
     1081 ccgatgggcg ccttcaaggt gacgtcaacc agttcaccct cgaagcgctc aaaatcctgc
     1141 gcgtgccgca aaggccggtc aatgcccggc gaagacactt caagccggtg gtaatccacg
     1201 ccctccacct caaggacata ctgcaactgg cgcgtggcac gctcgcagtc ctcaacggtg
     1261 atcagccgca cggcttcatc gggtgaccac ggaaaatcaa tggtgactcg cagcagacca
     1321 ccggcggagc gctcaagctc caccaactcg aagccgaggc cccggatcgt gtcgtctatc
     1381 gtctgctgca gtgccacgtt gtccgaaaaa aagatcggcc cgccgacggt tgtcgaaagg
     1441 ccataaaaaa tgggcgggtc ttaaagccgc ccatgtggtc gtcgtcaatc gagcgaattt
     1501 tagcacggaa cgttggcatt ctcatggatc tatgcgccgg atggcttgat gccgcgcggt
     1561 ggtcctcgtc acgtaggatt gggcgataat ttttctttat ccagcgtgct gtaacttatg
     1621 aacaactctc cggcctcttc cgacgtgtcg gccctgctcc ccgaagtcgc tcaattgatg
     1681 gtgaccgcac tcaacctgga aaccgcaccc gaggagattc agccggatgc acctctctac
     1741 ggtggcgatc ttgggctgga ttcaatcgac attctggagg tggccttggc ggtctccaag
     1801 aaatacggct ttcaattgcg ggccgacgat gaggacaacg ccgccatcta cgcctctctg
     1861 cgcagcctga cgcagcacgt cgcggcgcac cgcaccacct gacgccgcaa ccggtcgcac
     1921 ccgccaagcg gtgccgactt ctaccttccc catgcaagcg cagtacccgc tcagcagcca
     1981 cgcaccggac gacatcctcg cctaccgtca tggccgaggc gtggccgccg gtcgtttctt
     2041 ccaggacgca ctgtccctgg cgtcaacgtt ggtccccggc ggccacgtca tcaacgtttg
     2101 ccacgaccgc tatttgttcg cggtcgggct ggcggccgga ttgctggcag gcaaggtcac
     2161 gctcatgccg ccgagcttcg cgccgcaggt cattgcgcaa atgcagacca gatatcccga
     2221 cctgcggtgc ctgcacgatg accgcgagcg gcccgaaggc ttggccggca tctccgttca
     2281 ggatcatctg ggcggcaata cgtcgcccag caacgcttca ccgttgatcg acgccgggca
     2341 gattgcggcc atcgtcttca cgtcgggctc cacgggcgag ccgacggcac accaaaagac
     2401 ctgggggcgc ctttgcatca acggcgcggc cgagtcggcg cgcctgggcg ccgcaggcgt
     2461 gaccattgtc gccaccgtgc cggcgcagca catgtacggt tttgagtcga gcgtgctgat
     2521 ggccctgcat ggcggagcca gcttctggca tgggcgcccg ttttatccgg ccgacatcgt
     2581 cgaagccttg gcgtcggtgc cgaggccgcg catgctggtc agcacgccgt ttcatctgtc
     2641 gaccttgttc gaagctggtt tgaagttgcc tgcatgcgat ctggttctga gcgccacggc
     2701 accgctgtcg gctgagttgg ccgagcgcgc cgaagctgtc atgggcgcgc cgttgcttga
     2761 gatctatggc tgcacggaaa gtggccaggt ggccagccgg cgcccggtca atgggccggc
     2821 ttggcagctg ctgccaggcg tgcaaatggc cacggatgca gaaggcgcgt gggtttttga
     2881 cgggcatgtg gaagggcgcg tgctgctgtc cgaccgcatc gaagagcagg acaccgaaca
     2941 tttcttgctg ctgggccgca acgccgacat ggtgaacgtg gccggcaaac gcacatcgct
     3001 ggcccatttg aacgcacagc tggcaggcat tcctggagtc gtcgagggct gcttcataca
     3061 accagacgac gaggcggtgg cccgtgatgg caccgtccag cgcctggccg cgctggtggt
     3121 cgcgccaacg ctcgggccgt cggaggtgct ggcggcgttg cgtcagcgca ttgatccggt
     3181 ttttctgcca cggcctttgc tgcgtgtgga cgccatgccg cgaaacgcca caggaaagtt
     3241 gatccgctcg gacatcatgg cgctgtaccg gtcggccaag cgcgactcgc caccggaaat
     3301 tgcatgacga cccaggagta ccttcaatgg ccgctgctgg ccacgtggcg ggtgcccgat
     3361 gaacatccgg tgctgcctgg gcattttcca gcctatccgg tgctgcccgg cgcgttgctg
     3421 ctggattggg tgattggtct ggtgtcgagt taccccggcg acgctgcgat ccaggtgcgc
     3481 gcggcgcgct ttcccgcagc ggcgcgagcc cacatgctgc tgcgcgccca cgggcttcgt
     3541 gaaggcggtc gcgtgcagtt tgccgtgatg accgacgatg gcagcccgtt gtgcacaggc
     3601 actgtggacg gggtgaataa agggtcgttg tcgtcatgac ccctcccaca caagtggcca
     3661 gcgccgaatg ggcgactcgg ccagaacgca gttcgatggc cgtgctgcgc ctcatggtgt
     3721 ggctgtctct ttttttgggg cgccgcctca gccgccttgt tctctacccg attgccgcct
     3781 atttcgttgt cttcgcgccg aaggccaagc gggccagcaa agcctatctg agccgcgcgc
     3841 tgggacgtac ggcgcgcttg tcagacggct gtcggcacgt gctgacgttt gcctcggtgg
     3901 tgcacgaccg tgtttattgg ctgcgcggcc gccatgactt gttcgagatt gaagtgatcg
     3961 gcgaatccca tctcgccgcc ttgcgccagc agggccgcgg catggtcttt gcaggcggac
     4021 atttcggcag cttcgaggcg ctgcgggcgg ttggcgagcg ccacggcatc gacacccgaa
     4081 tgctcatgtt tcccgacaac gcccgcaagg tgacggcggc gctgacatcc atcaatcccg
     4141 cgctcgccaa cagtgtgatt gggttgggcc ggcccgactc cgtgctgcgc gttcgcgacc
     4201 acctgcagaa tggcggcagc gtcggtattt tggccgaccg catgctgggc gaagacaaga
     4261 gtcgcgatat cccgtttctc ggcgaaccgg cgccgtttcc cgtgggacct tggcgcttgg
     4321 cggcgatgct ggctgctccc gtcgtgttca tggcggggtg ctatctgggg ggcaatcgct
     4381 accagcttgt gtttgagcca atcatcgatt ttgcgcaggt gccagccgaa aaacgagcat
     4441 cggcgatgca agaggcgcag gatcgattcg tcgaactgtt ggagtcgcgc tgtcgtcaag
     4501 caccatggaa ttggttcaat ttctatgatt tctggcatgc ccagacctga acaaatgggt
     4561 caccccactt ggcgcaccta cttggtcgcg cccttgctgc tgatcaccag cgccagcgca
     4621 tgggcagaat ggaacgtcgc ctcgctgatg caacaactgg cgcgccaccc ggcaggtcag
     4681 gcgcgtttca ctgagaccaa gaagctctct gtgctggatg caccggtcgt cagcagcggt
     4741 cgactgatat acagcccgcc agatcggttg gaaaagatca ccgtgcaacc caaagccgaa
     4801 tcgctgaccg tcgaaagcga tcaagttgtg gtggtgcgcg atggccgccg gcgggaaatg
     4861 cgtctgcagc aagttcccga ggcgctggcc atcgtcgagg gcgttcgggg cacgctgctg
     4921 ggcaacgaag ccatgctgcg acagtattac gacctgcgcc tggtcggcac cgaaaaggac
     4981 tggcgtctgg cgctgattcc tcgcgaagaa cgcctggcac gctgggtgcg ccagatcacg
     5041 gtcaacggtc agcgccacgt cgtctccagc atcgaaacgc tgcaagccga tggcgacagc
     5101 tcaatcatgc ggatcgaggc cgagaaatga aaacggcgcg agcgctcctt ccgttttgct
     5161 gacctccagt tctcgcaccg acccgccgcg cagtcacacg acacgattcg ttctcggctg
     5221 gtggctggtc ctgatggcgc tttgcgtggc cgtggtcgcc acaagccgct acagcaccga
     5281 catgtcggcc tttttgccgc gcgagcccga tgcgcgacaa cgcctgctgg tcgatcagat
     5341 caaggatggc gccctttccc gcatggtctt gctcggcatc gacggtggca cacctgccgc
     5401 gcgcgccgag gcgtctgcca agctggcggc gaccttgcgg aaaagcacag aattctccgg
     5461 cgtggtcaac ggcgacgccg cgagtcgcga agtggatcag gcgcttctcc tgcgctatcg
     5521 ctacgtgctc agtcccaaag tcaatgccga gcgcttcagc agccaggggc tgcacgatgc
     5581 catcagccac acactggtcg agttgtctgg atcggccggc ctgttcctca agtcgctcct
     5641 cccgcgcgat cccaccggcg agttgtgggc cacgcttgaa agcgttgccg ggggcaatgc
     5701 accgtcgacc agcgatggtg cgtgggcgtc ggcggacgga cagcgcgcgc tgctggtgac
     5761 catgacacgc gccaaaggca ccgacaccga cgcgcaggaa cgtgcgctga ccgtcattcg
     5821 caagagcttc gacgccttga acaccgacgg cacgctgcag cttcgcatgt cgggcacgcc
     5881 ggtgttcgcc gtgcacgcac gcagcaccat ccggtcggaa atcgagcgcc tatcgttgat
     5941 tggcggcctg agcgttttcg ccctgttgtt cggtgtgtac cgatcttggc gcaacgtcgc
     6001 catcggcttg ctgcccgtcg tgtccggcat tctggtggcc atcgccgccg tggcgctcag
     6061 tttcgacacc gtgcatgcca tcacgattgg atttggcacc accctcattg gcgaagccat
     6121 tgactacagc atctactacc tggtgcaggc gcacgacccc caagcgtggc gtaccaccta
     6181 ctggccaacc atccgcctgg gcgtggccac ctcgctatgc ggtttcgcgg cgctgctgtt
     6241 ttccagcttt cccgggctcg tgcagttggg ggcctattcg gtggcggggc tgatcgcggc
     6301 ggccatggtg acgcgcttcg tgctgccggc gttgaccacg gcgccggttc cgtttgcgcg
     6361 catcgatcgc ctggggcgcg cagtcgcctg gctggccgcg tggatgcaac gcctgcgctg
     6421 gctggcggtt gggctcaccc tcgcggcgct cgtgttggtg ctcgtccagc gcgatcacct
     6481 ctgggcatcg ggactctccg ggctcaaccc agcgccgctg gacatgcaga agctggacgc
     6541 cgagttgcgg cgcgacgccg gcacgcccga tctgcagcac atggtcgtgg tgactgcaga
     6601 gaccgaagag gcggcactgc gggccgctga gcaggccgag caaagcttgc agccgctgct
     6661 ggccagcggc cgcctgtcgg gcatcgacag cccggcgcat ttcctgccca gccagatcgt
     6721 ccagcacgag cgcctggctg ccctgccggc ggctgatgaa ttgcgtagcc gcctgaccag
     6781 cgcgctgaag gacctgccgc tgaagatcga ccggctgacg cccttcgttg aggacgttgc
     6841 aagggcaaag aacgaaggcc cattgaccgc cagcgccctc gcgggctcca gcttcgcctt
     6901 tgccgtgcag acgctgctgt tgccgcgtga tggtcaatgg tcggtgctga tgccactgcg
     6961 tttgccggca gcgggcgcct cgaccaatac agaagatctg aagagcgccg tcaccacgct
     7021 gctgaattca tcccctcctc gggtcgaggc gttttacgtc aatctggacg agcaggcgac
     7081 cagcgttttc ggccaatacc tgcgacaggc cctgatgctg gcgctggcgg gcagtctggc
     7141 cgtgctgctg ctggtggccg tagcgctgcg cgacttcaga cgcacgatcc gggtgttggc
     7201 accgttggtc ggcgcggtgg cgctggtcat ggcggcgcat ttggcgcttg gcgtccgcat
     7261 gacgctgctg cacctggttg gcttgttgct gatcgtggcg gtgggctcca actacgccct
     7321 cttcttcaac cagcgtgttt cacagacgag tggcgcagcg ctgccgcgca cgaccgccct
     7381 cgcctcactg gccctggcca acgtatccac catcatcggc ttcggtgtgc tggcgctgtc
     7441 gaacgtgccg gtgttgaatg ccattggcgc cacggtgggc cccggcgcgc tgctggccct
     7501 gctgctggcc atgagctggg cggcgggcga tgcgcgggcg gcgagcccac ccggagcgga
     7561 ggcggcgtga aaggcgcggc acctggttct gcttcgaccg cgtcgtggct caccgaactg
     7621 cggcgccgca caacaacgct gccctggctg aaagctgcgg gcaacgccgt gttcctgatc
     7681 ctgttttttc gcgcctatct ggcgattcag cgcaacccgg tgttcgaggt gatgcagatc
     7741 ccggccacgg ccatcgatcg ctgggtcggc tttcagccct ggacgctcgg gctctacctc
     7801 agcctgtggg tttatacggc gctgccggtg gccttgcagc ctgacctgcg ccaactggtg
     7861 cgctactgcg ctcacatctc cgcaatgtgt ttgctcgcgc tggcggtttt tttcctgtgg
     7921 ccgacgtccg tgtccgatgc cgtcggcccg cggggcggcg gtggcctttt tgccgctatg
     7981 tacgcggtgg acgacgccgg caacgcctgc ccctcgctgc acgtggcggc gtcggtcttt
     8041 tcctttctgt ggttgcgcgc catgctggcc gatgtgggcg cgccgcgctg gttggcgttg
     8101 atcaacgcgt gttggtgcgt gggcatctgc tactccacgc tggcgaccaa gcaacacctg
     8161 ctgatcgacg tgctggcggg cgcggcgctg ggtggggtgg gcggttggtg gagcctgcgg
     8221 gcctcagggc gatcaaaaca gcgcgtgcaa taatcaaaaa gcgcagatgc gcgtgtctct
     8281 ttcggcgtag ttcgcagtcc atgcagtcct cccttcctac cgatcaggcg ggcgattcgc
     8341 ccgttgaatg cgacgttctg gtcattggcg gcggcccggc cggcagcacg gtggcggcgc
     8401 tgctggcgca aaaaggccgc cacgtagtgc tgctggacaa ggatcgccac ccgcgttttc
     8461 acattggtga atcgctgctg cccgccaacc tgcccttgtt cgagcgcctg ggcgtggccg
     8521 atgaaatgcg cgccatcggc atcaagaagc gcgcggccga attcatgtcg ccgtggcacg
     8581 aacacaagca gcagttcctg tttgccgacg cgtgggacaa gaccatgccg cacgcctatc
     8641 aggtgcgccg gtccgagttc gaccaggtgc tgctgcgccg cgccacgcaa ctgggcgccc
     8701 atgtgcgcga aggctgcaag gtgaccgcgg tcgatctgga agccacgccc gagcgcgcca
     8761 gggcccaggc gcgttgcgac gacggccgca cactcgctta cgcaccgcgc ttcgtggtcg
     8821 atgcgtcggg ccgcgacacc tttctggcca gccgtttcca gatcaagcac aagaacccgc
     8881 agcacaacag cgtggcggtg tacggccatt tcgaaaacgt gcgccgcaac gaaggcgagg
     8941 acgaaggcaa catcatcatc ttctggttcg agcacggctg gttctggttc attccgttgc
     9001 aagacggcgt gaccagcatc ggcatggtga cctggcctta tttcatgaag acgcgcggcg
     9061 agcgcagcct gcagcagttt ctgctggatg ggattcggca atgcccgccc ctgctagaac
     9121 gcatggctgg ggcgcgcctc accgacgtga tcgaggccac cggcaactat tcctacagct
     9181 gcgagcgcaa ccacggccgc aactacgtgc tgctgggcga cgcctacgcc ttcatcgacc
     9241 cggtgttttc gtccggcgtg atgatggcca tgcagggctc gttcgacgcc gccgacgcca
     9301 tcgacagctg gcttgaccac ggcgccgagc ccgcgctgca agccgcggcg ctgcgtaagc
     9361 tggatgcgcg gctgcgccac gggccgcgca ccttctcatg gttgatctac cgcatgacca
     9421 acccggtcat gcgcgatctg ttcatgggcc cgcgcaaccc gtttcgcgtc aaggaggcgc
     9481 tgctgtcgct gctggcgggc gacatctacg gccgcacgcc gatctggact tcgctgcgca
     9541 tcttcaagct gatctactac acatcgtcgc tggtccagtt gccgacggcc ctgcgcgcct
     9601 ggcgccgtcg ccagaccaat ttgcgcccgg tcgacgaagc ggccggcgcc aggtagcacg
     9661 ccgcgacctc cttgccctcg ccaaaccacg aattgtgctg aagctgctct ggcgtgccta
     9721 cgaacacgtc gccatggtgg tgggcttgct gctgcttgcc acggtctgcc tgacctggac
     9781 gccgttcgcg ctgctgctca atccgctgct gcccgaggcg cgcgccaagc gcgtcgggcg
     9841 cggcgccatc cggggcgcgt ttcggctgta cctgagattg ctttcgctgc tgtgcgcctg
     9901 ccgcttcgat ctgcgcgaat tgcaggcgct tggccggcgt ggcgagcccc tggtggtggt
     9961 cgccaaccac ccttccctgc tggacgcggt gatcctggtg gcctgcctgc ccaacgccgt
    10021 gtgcgtgatg aaggcgcgcc tgatgcacaa cgtgctgctg ggtgccggcg cgcgcatggc
    10081 gcgctacatc gtcaacgatg cgccgctgcc catgattcgc ggtgccatcg ccgaactgcg
    10141 ccagggcgcc tgcctgatca tctttcccga aggcacgcgc accgtgacgc caccggttga
    10201 tgcctgcgcc agcaccgcgg gcgtcatcgc cgcgcgcgca ggcgtgccgg tgcaggcgct
    10261 gctgatcgag atgagcacgc cctacctcgg caagcactgg ccgctgtggc gcccgccggt
    10321 gttgccgctg agctgccgcg tgcgggtagg ccagcgctgg gcggtgctgg agcggccgca
    10381 gcagttcggc ccagaaatcg aagcctactt tcgcgaagcc atcgacccgg ccgccaacca
    10441 gttgcctgac gcccgcgaat gagcgcatcg cccgcctcca ccacgcacct ggtactcatc
    10501 cccagctaca acccgggcgc caaggtgcgc gatacggtgg cggcggcgcg cgcggtgtgg
    10561 gcgccggtct gggtggtggt ggatggcagc accgacggca gcgccgactg gctgcgcgag
    10621 atggcggcgc gcgactccgg cctgcgcgtg ctggtgcagc ccgaaaaccg cggcaagggc
    10681 gcggcggtgc tggccggcat cgagcaggcc gcgcgcgagg gcttcaccca cgcgctgacc
    10741 atggattcgg acggccaaca tccggccgag ctgatccccg agttcatgtg cgcctcgcag
    10801 gccgcgccgg ccgccatggt gctgggcgtg ccggtgttcg gcgccgaggc gccgcgcatt
    10861 cgcgtgctgg ggcgcaaggt gtccaacggc tgggccaacc tggagacgct gggcgccggc
    10921 attggcgatt cgctgtacgg ctttcgcgtt tacccgatcg cgccgctggt gcgggtgatg
    10981 cgcgggcaac gcttcatgcg ccgcttcgat ttcgaccccg aagccgccgt gcgcctggcc
    11041 tgggccggcg tgcatccggt caacctgccg gcgccggtgc gttacttcac gcctgaagaa
    11101 ggcggcgtgt cgcacttcaa atacctgcgc gacaacacgc tgctgacgtg gatgcacacg
    11161 cgcttgttca tcggtttttt gctgcgccta ccggtgctgc tggcgcggcg aatttttaga
    11221 tgaaaagtgc cgccagcgct tgtctatcaa gcgcaagcag ctactatttt tatagtatca
    11281 actgcccggc gcgcagcgcg gcaaccaacg gcgcagcagc ggcgacgtga tcaccgtgct
    11341 gatgatcgcc atcagcacca gcatggtgaa caggttctgc gagatcacgc ccaggtcgta
    11401 gcccacgttg atgacgatca gctccatcag cgcgcgcgtg ttcatcaaga tccccagcac
    11461 cttcgattcg gccttcggta accccacgaa gcgcgccgcc aggtagctgc cgcaaaactt
    11521 gcccaccgtg gccaacccga tcaccaccgc gcaccagccc cacagcgcag gcgtgtccag
    11581 cccgccgatg ttggtgcgca agccggtgta ggtgaagaag atgggcagga agaacacagt
    11641 cacgaactgg cccatgcggt cgcgccaggt gtcgaccagc tcgcgctcgt cgtgcagcag
    11701 aacgcccagc atgaagccgc cgaagatggc gaagatgccg agcttgtagg tcgtcatggc
    11761 accgatgaag atcgccgcga tcagcaggcc cagcaggttg ttcgacaagg ctttgggtgc
    11821 gccgtcgcgc gcgggctggt tttgcgccca tcgcaccagg cgcttgagca gcggccgcgc
    11881 gatccaccac gacagcacgg caaacagccc gaccgcgccg acgttgagcg cgaagccgcc
    11941 cgcctgaaac tgcgacaccg acagcgccgt gaccagcgcc agcagcagcc agccgaccac
    12001 gtcgttgatg gcggcagcgc tgatcgcgat gacgcccagc ggtcgttgcg tcatgtcgaa
    12061 ctcgatcagg atgcggccca ggatcggcag cgccgtgatc gagaacgagg tggccacgaa
    12121 cagcgccgag gccagcgcct gcccgttccg gccgccgaga aaaggcgcga cgacccagcc
    12181 cagcgccaag ccgagcgcaa acggcagcag caagcccgcc gccgccaccc acatcaccgc
    12241 gcggcggttg cgccgctcgg tcaggtggcc aaaatcaaac tccagcccga tctggaacat
    12301 cagaaaaatc agaccgatct gcgacagcac ctgcatcggc tcaccgggcg ccgaatgaaa
    12361 cacgtagttg aaggtgccgg gcgcgagcag gccgaacagc gacggcccga gcaggatgcc
    12421 gacgatgatc tcgcccaccg ccgtcgactg gccaaagcgc acagccaggc tgctgccgat
    12481 gcggccggcc agcaccacca ccgtgagttg cagcagggtg aagaacaaga ccgcttcggc
    12541 cttgtgaacg ccgatttcct gcgccgccgc ttccacactc attggtacat ggccccgttg
    12601 atgccgatgg tctggcccga gatgtaggcg gccttgtcag aggacagaaa agccaccaga
    12661 tcggcgacct cttgcggctg gccggcgcgg cccatcggca ccatgcgctg gatggcggcg
    12721 gcatcaaacg tgcccgcgct catctcgctg gcgacgatgc caggcgccac ggcgttgacc
    12781 gtgacgccgc ggctcgcgca ttccagcgcc agcgatttca ccgcgccgtg cagcgccgac
    12841 ttggcggcgg cgtagttcac ctgcccccgg ttgcccgcct gccccgccac cgaggtgatg
    12901 gccacgatgc ggccccagcg cgtgcgcagc atgggcagca tcagcggctg ggcgacgttg
    12961 aagaagccgt tcaggttgac gtcgatcacg cgctgccact gctcggggcg caggccggcg
    13021 aacacgccgt cgtcgtgcac gccggcgttg tgcaccagca cctgaatcgg accggcttcg
    13081 agcatttcgg ccagtgcctt ggagcaggca ggaccgtcgg tgacgtcgaa caccacgctc
    13141 tcggccgcgc cgcccgtggc gtggatagcg gccaccacct cgtcggccgc cgcgcggttt
    13201 cgattggcat gcgccagcac atgcagcccc tgcgccgcca gcgcgcgcgc aatggcgccg
    13261 ccgatggcgc cgctggcgcc ggtgaccagg gcgcgtcggg tgggtgaaga atggttcacg
    13321 gcgagaccgg caccggcacg tcaagcatca cggtggcgcg gccttccacc agcagttgat
    13381 cgcccgcatg cagcgaaaac gcgtactggc aaaggtcgcc gttctcggcc tcgcagcgtg
    13441 ccgcgacggt gatttcgccc gccacgtcat cgagccgcgg cacgtgcaga gtcacgccgc
    13501 gcacgctcac cagcaggccg cggcgcgggc gtggcacgcc gcgctgctcg gccagcaagg
    13561 cgccatgcaa ggccatggcc tgcgctgcgt attcgacgcc gcaggccccg cccaggcggc
    13621 cgtgctggcg catcgggtgg tcgggagcgt ggtggctgtc ggcggcgcag cggatgccct
    13681 cgggcgaggc gcccagcacg cggcccagca ggcacatgga gccctggtgc ggcacgcggg
    13741 cggcgatcca gtcgtggccc tggccgctgg cctgtgccca ccacgggctc gtcacggcat
    13801 cgctcatgcg ggcgccacgt ccagccacag ccattggggc ggccaatacg gcagcgccac
    13861 gtggcgcgca gcgccgccgg caagcgcggc cagcaacggc agggcgcgca tcggcggcat
    13921 ggcatcgacc agtgcggcca gtgtcgacgg cagatgagtc aggtcgcccg ccgtatcccg
    13981 cagcgcgccc tggcgcggca gcagcagccg gccgagcgag cgcggcccct gggccggcgc
    14041 cagcagcaag gcgaaggcgc tggtgtcctg aatcgggcgc ttggcgtgca gcggctcggg
    14101 gtattcgctg tcggccgcca ccagcagcac cggcgcaccg ctggcgtgca cctgcgtaac
    14161 ggcctccagc aggccggcgg ccacgctggc gtcgtaagcg cccagcacct gtgccgccgg
    14221 cgtggcgcca gtggcaatcg accagtagcc cgacgccgcg ttgtgcaccg agttgtgaaa
    14281 gcgcgtgggc gaaatctgcc ggtcatcacc ggccagcagt tcgcacagct cgtggccgtt
    14341 gtggccgtcg ccgccggagc tcgcaaacac gttggccagc gtggcggggt cggcgccggc
    14401 ggcgcggcag gcctccagac ccaccgccaa ggccgccttg acgatgcggc tggcgcgccg
    14461 ccgctcggcg gcgggcagca cctctggcgc gggcaaggcg gtgggcgcgg ccgtgtacgg
    14521 cgcgtcgcca cgcagcagcg cgcggcccgt ggcccagtcg ccgatgccgg gcgccagcag
    14581 gccgatgcca tcgacccacg cggtgatcgg ctcgcgcgcg ctcatgcgcc cctccgcgac
    14641 agcaccaggc tggcgttgct gccgccaaag ccaaatgaat ttgatagcac gtggcgcagg
    14701 ctggccgtgc gcgggagcat gatcagatca acaggaatcg ccggatcggg cgtgcgcgtg
    14761 ccgacgctgg gcggcagcaa gccgtgttcg agcgccagcg cacaggccac cgcttccagc
    14821 gcgcccgcgg cgcccagcgt gtggccggtg atgcccttgg tcgagctgca gggcacgcca
    14881 ccgcccaagg cggtgccgaa cacgccggac acggccagcc cttcagccac atcattggcc
    14941 ggcgtggcgg tgccgtgcag gttgatgtag tcgatatcgc ccggcgccag cccagcccgc
    15001 gccagcgcac cctgcatggc ggcgcgcgag ccacggcctt cggggtgtgg cgccgacatg
    15061 tggtgggcgt cgctcgcctc gccccagccg ctcagcacga cggcggcatc gcccggttcc
    15121 agcagcgcaa aggccgccgc ctcgccaatc gacaagccct ggcgcgccgc atcgcccggg
    15181 cgacacggtt cgggcgaggt cagctccagc gaagcaaagc cgtacagcgt ggtcaggcac
    15241 agcgaatcga cgccgccgac gacggccgca tcggccaggc cggcggcgat cagccggtcg
    15301 gcggcggcaa acgccttggc cgacgacgag caggcggtgg agatggcaaa cgccggcccg
    15361 cgcacgccga gcagctcgcg cacgaaagcc accagcgagc cggtgttgtg agtgcgcgcg
    15421 taatcgaacc aggccggcag cgcaccgccc gcgtcgcgtt cgcggtaggc caactcggcc
    15481 gacaacatgc ccgacgtgct ggtgcccaga atcagcgcca cgcggtcggc gccgtggcga
    15541 gcgatggcgg cgcgggccgc gtcctcgaag ccatcggcgc gcaggcccag cagcgccagg
    15601 cggttgttgc ggcaatcgta ggcggccagc ccgggcggca gcgccacctc gtccacgccc
    15661 ggcacttcgc ccatccaggt ggccagcgcg gcggtttcaa agttgcaggc tttcagcccg
    15721 ctgcggccgg cgcgcaaggc gtccagcaag gcggcgcggc cgtggccgac ggcgctgctg
    15781 gcggtgtagg cggtcagtgc cagtgacatg aatcagcaac cctcgggagg cacgcgagcg
    15841 accaacatgg tgttggcaaa cggcgggccg ccctgggtgc gcaccacctg cacgctgaag
    15901 cccagcgcgc gcagcgcctc ggcccagtcg gcggccgtgc ggcaatacat gggcgacagg
    15961 ccgtggccgc gcaccagcgt caccgcctgg tcgacgcgcc ggctcagctc aaagcgccag
    16021 ccggccgcgg catcgcccac gcgcgccaac agcaggctgc cggcgcccaa ggccgcgcgc
    16081 atgcggcgca gcacgtcgtc ttgcgcctcg ggcgggaagt agtgcagcac gtcgaagatg
    16141 gtgatcacgt cgaccggctc gaacacggcc tggcagatgt cgccctgctc cacccgcacg
    16201 cgcggctcgc cgtcgaaggc gcgctggccg cgcgcgacgt cgcgcggcat cagctcgagt
    16261 gcgtgcaggc tttgcacacg cggcggcggc ccccatccgg ccggccattg accggtggcg
    16321 tggtgttctt cagccgccag cagccacgcc gccagcaccg cttggccgca accgaggtcg
    16381 agaaagcggc cgtgcgccgg cagcaagccg agccgcagca aatcgatgaa cagcgggtcc
    16441 atgcccagct tgccgcgcgc gaaatgccag gcaaagcgac cgcccgcacg gtagcgggcg
    16501 ctggcggcgt cgaccagcgc cagttgcagc gcacgcggat cgggccgcgc cgcgccggcg
    16561 gcaaccaagg gcggtgcttc ggcgcgcgtg ggggccaggc gctgttcggc gctgggttcg
    16621 gcttgcagcg tcatgccggc accccttgcc tgggcgcggg cacgggggcc ggtgcgcaag
    16681 ccgcatccag ggtcacgggc ttgaggccgc gcgcgcgaag tgccgccagc aggggcggca
    16741 gcacggccag caccaccggt tgccccgctg ccgtggtggc gccgtggccg tcgtgcagca
    16801 acaggatgtc gccagcagcc aggccgcgcg tcaggcgcgc cagcacctgc gcggggtcgg
    16861 cgttgcgtgt gtcgtagccg cgccgggtcc agctggccag ttgcaggccg tggcgcgcca
    16921 ggatgggttc gaggaacggg ttgcgcagcc cggccacggc ccgcacgaag cgcggcgcgc
    16981 ggccgctggc ttgcgccagc gtgcgttggg catccagcac ctcgcgcgca aggcggcgcg
    17041 ggccgtagag cgacagcgcg tgcgggtggt gctgcgtgtg gttctcgatg tggtggccgc
    17101 gccgcacgat ctcgcgcgcc agcgccgcgt gggccgccac gcgctcgcca atgcaaaaga
    17161 agctggcgcg cacgccggcg gcgtccagca tatcgagcac ctgcggggtg atggccgggt
    17221 cgggaccgtc gtcaaaggtc agcgccactt cgccgcgcgc ggcggcggcg ggcggcaggc
    17281 gcgtcaggtt cgggcccagc cagttgcagc gcggcagcag gccggcagcg gtgatcaggc
    17341 catggttgac gatcagcgcg ccaagccacc aggcccattg cgccggcgcc aacaacaagc
    17401 caatgccggc cgccaggtgg acggccgccg agagcatcaa aaaggcgctg ggccgccagc
    17461 gggcgggggt gcgttcgggc acagacatcg gcggagatta tggcccatgc cgtcatctgg
    17521 cccgtgccgc cgctgttcgg catgaaatcg gccgcctgcg cttgtccagc aagcgcaagc
    17581 agctataaaa atgatagaac tttcaggcta agccgccgcc agcagcttgc gggtgtacgg
    17641 gtgctgggcg tgaccgagca cctgggcggc gctgcccgcc tcgaccaccg cgccgtcttt
    17701 cagcacgatg acgtcgtgcg ccagggcgcg caccacgtcg acgtcgtgcg tgatcagcag
    17761 gtagctcagg gcgcgttctt tctgcagctt ttgcagcagc gccagcacct gcttctggat
    17821 ggtgacgtcg agcgcgctgg tgggttcgtc cagcaccagc agctcagggc ccacgatgag
    17881 cgcgcgcgcc agggcaatgc gctggcgctg gccgccgctg aattcatgcg ggtagcgcgc
    17941 cagcagattt ggaaactgcg tctcggtcaa accgacttcg gccaaagccg cgcgcacccg
    18001 cgtggcgcgc tcgggcgccg gcagctcggg tgcgtgcacc agcaggcctt cgccgacgat
    18061 ttcctcgaca gtcaggcgcg gcgagagcga cgaaaacgga tcctgaaaca ccacctgcgc
    18121 cacgcgccgc agcggcttgt cggcggcggc tttcagctgc cagggccggc cggcgacgag
    18181 cacgctgccg cccacctgcg cacgcggcag caagcccagc gcggcctgcg ccagcgtgga
    18241 cttgccagag ccggattcgc cgatcacgcc cagcgtgcgg ccgggcagca ggcgcaaatc
    18301 ggcgccttgc agcgccacga attcaccctt cttgaaccag ccgcggatgc cgggcagcgg
    18361 cgtcgggtag accacgcgca gttcgtgcgc ggccagcgcg ggggtatcgc ccggcgcggc
    18421 gggtgtttca accacgtcgc gctcgggctt gctggccatc agcttttgcg tgtaggcgtg
    18481 ctgcggcgcg ctgaagatgt cggcggtggc gccctgctcc accagctggc cgtgctgcat
    18541 caccgccacg cggtcggcaa agcggcgcac caggttcaaa tcgtgagtga tcagcagcac
    18601 cgccatgccg ttggcgcgct gcaactcgct cagcagatcg aggatttggc cgcgcaggct
    18661 gacgtcaagc gcggtggtcg gctcgtcggc cagcagcagc ttgggctcgc acgccagcgc
    18721 catggcgatc atggcgcgct ggcgctgacc gccgctgagt tggtgtg
//
LOCUS       DAOMVO010000015        18526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_562_length_18526_cov_12.831032, whole genome shotgun sequence.
ACCESSION   DAOMVO010000015 DAOMVO010000000
VERSION     DAOMVO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_18526_cov_12.831032"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PKV30_01725"
     CDS             complement(<1..128)
                     /locus_tag="PKV30_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL28972.1"
                     /translation="MRLNSIKLSGFKSFAEPTNFMLPGQLVGVVGPNGCGKSNIMDA"
     gene            complement(318..1229)
                     /locus_tag="PKV30_01730"
     CDS             complement(318..1229)
                     /locus_tag="PKV30_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRL28973.1"
                     /translation="MNRESLSAVAIGAPLHAGSGAERASTEPDAAPERRPSHWRVEAL
                     DFAAIDPARIAAREDLFYLLVSASFIESGSDLYTRNLSDHYADYPEVAAWLLEHWEHE
                     ELQHGRAFECYVKAAWPEFPWQAAFDGFIAEYGPLCTMEELEPNRALELAARCVVETG
                     TTTYYQTLRDLSDEPVLTDMLGRIRADEVSHYKHFLAYFKQLHAAQPASRLRVARVVY
                     RRLLELRESDADVALRHVWAHKGRMFADGAKSFDEISQRVSQLVSSRLPADQAVRMLL
                     KPLMLPRKLESWIERPLASLARRVVAS"
     gene            complement(1302..1541)
                     /locus_tag="PKV30_01735"
     CDS             complement(1302..1541)
                     /locus_tag="PKV30_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28974.1"
                     /translation="MSQACSTCGNVYDKAFTVTASGQTYTFDSIECAAQKLAPSCEHC
                     KVRILGHGLEAGDGRFFCCDHCAEKAGVKGLRDRV"
     gene            1652..2857
                     /gene="dapC"
                     /locus_tag="PKV30_01740"
     CDS             1652..2857
                     /gene="dapC"
                     /locus_tag="PKV30_01740"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HRL28975.1"
                     /translation="MNPLLSRLQPYPFERLRKLFEGVTPNPAYRPISLGIGEPKHATP
                     QFIQDALAGDLATGLSGYPPTAGSPALREACAGWLQRRYGVTVDAATQMLPVLGSREA
                     LFALAQVVVDPTKPNATVVCPNPFYQIYEGAALLAGAEAFYVASDPARNFAPRWDQVP
                     AEVWARTQLVYVCSPGNPTGAVMPLDEWKLLFELSDRHGFVIASDECYSEIYFRDEPP
                     LGGLEAAAKLGRTDFKNLVMLTSLSKRSNVPGMRSGFVAGDAAIMKALLLYRTYHGSA
                     MSPVVQTASIAAWGDEAHVEDNRRLYREKFAAVTPVLARVMDVKLPDAAFYLWAGVPA
                     AWNSSDTDFARELLAQYNVTVLPGSYLAREANGANPGQGRVRMALVADTAECVEAAER
                     IVQFIQSKS"
     gene            2866..3699
                     /gene="dapD"
                     /locus_tag="PKV30_01745"
     CDS             2866..3699
                     /gene="dapD"
                     /locus_tag="PKV30_01745"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRL28976.1"
                     /translation="MTQQLQQIIDAAWEDRANLSPTKAPKEVADAVEHVISELNAGRL
                     RVATRESVGKWTVHQWIKKAVLLSFRLKDNDFIRAGDLGFFDKVQTKFAHLDESAMRE
                     SGVRVVPPAVARRGSFIGKGAVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPLQAGPTIIEDNCFIGARSEVVEGVVVEENSVLGMGVYIGQSTPIFN
                     RETGEMSYGRVPSGSVVISGGLPKKCADGTPYNTYAAIIVKTVDAQTRSKTSINDLLR
                     P"
     gene            3782..5071
                     /locus_tag="PKV30_01750"
     CDS             3782..5071
                     /locus_tag="PKV30_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRL28977.1"
                     /translation="MATSVPAPTNMMERILHLMAEKKASDVYLSAHAPATIKINGQCM
                     PINSQVMPPEAVMNLLMDVVPPNRIEELKETGELNMAIPMYGVGNFRLSAMRQRGTYA
                     AVIRFISPEIPDLDSLHLPSILKHLIMEKRGLILMVGATGAGKSTTLASMLDHRNKNA
                     SGHILTIEDPIEFIFTNRKSLVNQREVGSDTAVLQTGLKNALRQAPDVILIGEIRDRD
                     TMSAAIAYAQSGHLCLATLHANNSYQALNRVLSFYPAEVRPTLLGDLSAALKSVVSQR
                     LIRTVDGGRTPAVEVMLNTKLIQDLIEKSDFSGVKEAMDNAMAEGSQTFEQDLARLIL
                     EGKIDRKEGLANADSPTNLMWRLQNDSGRLNPNKPQEEERPSDEPSFTEITLDVKPSG
                     YRTTGFGKTGFGNSGFRDSGFQDSKMGGYSDSRIGGA"
     gene            5152..6318
                     /gene="dapE"
                     /locus_tag="PKV30_01755"
     CDS             5152..6318
                     /gene="dapE"
                     /locus_tag="PKV30_01755"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRL28978.1"
                     /translation="MPATRLLAEQLIARPSVTPLDAGCIELIAARLAPLGFVNEIIDS
                     GPDTFRVRNLWSKRPAAHAGQARAAIKTIVFAGHTDVVPTGPLEQWSSDPFAPTERDG
                     KLYGRGASDMKTSIAAFVVAVEELIVAQPNAPLHIAFLLTSDEEGPAVDGTVVVCNVL
                     KSRGERLDYCIVGEPTSVERTGDMIKNGRRGTLSGKLTVKGIQGHIAYPHLARNPIHD
                     LAPAFAELVAIEWDRGNAFFPPTSWQVSNIHGGTGASNIIPGSVVVDFNFRFSTESTP
                     ESLQQRVEAVLQKHGLEYELKWTLGGLPFLTTPGDLVTAVQAAIHAETGLETELSTTG
                     GTSDGRFIAQICPQVIELGPPNASIHKIDEHIALADIEPLKNIYRRVLENLAAA"
     gene            6315..7184
                     /gene="prmB"
                     /locus_tag="PKV30_01760"
     CDS             6315..7184
                     /gene="prmB"
                     /locus_tag="PKV30_01760"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRL28979.1"
                     /translation="MTLAELVADTERQLIEAGVSFGHGTTNAHDEAAWLVLHALGLPL
                     DTDIDGSEGKQPAAPDGQARTATLLKARIDTRQPLAYLLKEAWLQGVPFYVDERAIVP
                     RSLIAEVLADGSIDPWLSDQTHRVLDLCTGNGSLAVLAALAWPEVQVDAADLSVEALA
                     VARINVEKHGLKDRIKPIESDGLHNCPGPYDLILCNPPYVNVASMAALPAEFRAEPAL
                     ALAGGADGMDFIRALLRDAPTRMNERAVLVLEIGHERTHFETAFPRLNPVWLDTSAGG
                     DQVLLLTCEALAP"
     gene            7181..9316
                     /locus_tag="PKV30_01765"
     CDS             7181..9316
                     /locus_tag="PKV30_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL28980.1"
                     /translation="MIQLQNLTLRRGAKVLLDGASITIHPGEKVGLVGRNGAGKSTLF
                     ALLNGSLHEDGGDFSMPASWQIAQVAQHMPETTESATDFVLSGDERLSRLREQLSEAE
                     QSGDGMLIAHAHSDLHDAGAHDATARAQALILGLGFKVEELHRPVNSFSGGWRMRLQL
                     ARALMAPSDLLLLDEPTNHLDLDALVWLEGWLARYPGTLIVISHDRDFLDAVTRVTLH
                     IENGRLARYGGNYSAFEELRAQQLSLQQAAFSKQQDKIAHLQKFIDRFKAKATKAKQA
                     QSRVKALERMEKIAPVLADAEFQFEFKEPASLPNPMLAVRDGVFGYRSDGTTKSEAAG
                     ADLTSAGGSSDAKPALSDYATTATLNAQLAARGELPPEPQVGDRVILRGVSRTVLAGQ
                     RIGILGANGQGKSTFVKTIARTLPLLGGTVTEGKGLAIGYFAQQELDVLHADSTPLEH
                     MVRLARDTGRAHTEEAREQQLRNFLGSFSFPGEMVTQRVGQFSGGEKARLVLAMIVWQ
                     RPNLLLLDEPTNHLDLATREALAVALNEFEGTLMLVSHDRALLRSVCDEFWLVARGGV
                     APFDGDLEDYQRYLLDEAKRLRAAAADQVAADSIAVAAIPESAAAQKPLQEPLNPVNP
                     AEQRRLDAQRRQQLAEKTRPLKRALEKTEQRMQALGAEQATIEQRLATALPPADIAEA
                     GRRLKAVGDELATLEDEWLRLSGEIEAIE"
     gene            complement(9336..10328)
                     /locus_tag="PKV30_01770"
     CDS             complement(9336..10328)
                     /locus_tag="PKV30_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL28981.1"
                     /translation="MPASHRFSTRRAALATFAALVLAANAPALAQAPAGGAVKFILPN
                     AAGSGVDTIVRAAQPALAKALGQPVVIDNQPGASGVIGLQALSRAAPDGHTLSFVSNN
                     VVILPHVLKSVPFKMPEDFTPIAVVGITPLVLVVNPTKVPATNSKEFVALLKAKPDGY
                     NFASGGTGTILHLAAEMFVDEAGVKARHIPYKGVGQMVTDLIGGQVDFGVLGLVTAQQ
                     HVKSGALRIIGTSSGERLAAAPDVPTFAEQGLPGYVVDGWFAVLGPKGLPAAQVKRVH
                     DAVAAAFDAPDVKEAMAKQGNVIQIGTPEQALATFKAEGARFAALVKKAGVTPQ"
     gene            complement(10371..11582)
                     /locus_tag="PKV30_01775"
     CDS             complement(10371..11582)
                     /locus_tag="PKV30_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRL28982.1"
                     /translation="MSNQTNTNKRPRPLEGLRVVEFTHMVMGPTCGLVLADMGAEVIK
                     VEPIDGDRTRHLLGAGAGFFPMFNRGKKSIAIDLHTPEGAAVARKLALSADVVAENFK
                     PGTMTKYGLDYAALSAQNERLIYVSCKGFLPGPYEHRTALDEVVQMMGGLAYMTGRPG
                     DPLRAGTSVNDIMGGMFGAIGALGAIIQRGITGKGQEVQSALFENNVFLVGQHMLQYA
                     ITGEPAAPMPDRISAWAVYDVFTVKDGEQIFLAAVSDAQWRTFCDALGLADLRDLPDH
                     ATNNQRVRARPTLMPVLRERLAKRSAAELAALFEKVGLPFAPIRKPEELYDDPHLLAT
                     GGLSDITLTDGARAGQPAQTTLLPVTLGGERLGLGTQPPTMGRDTAALLVQLGYDAAE
                     IEALRAGHAVA"
     gene            complement(11602..12537)
                     /locus_tag="PKV30_01780"
     CDS             complement(11602..12537)
                     /locus_tag="PKV30_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRL28983.1"
                     /translation="MSTPSTLPRRAVIREVGLRDGLQAIARTLPTEQKKQWLRGAHAA
                     GQREIEVGSFVPAKYLPQLADTAELVAFAKTLPGLFVSVLVPNMRGAESAIESGADLM
                     LVPLSASHAHSLANLRKTPDEVVQDVARIRAARDAAGSKTLLEGGMSTAFGCTIQGEV
                     KHSEVLRLLQALLDAGCDRVSLADTVGYADPAAVQSLFEAARKVAGDKLWAGHFHDTR
                     GLGLANVYAALQTGVARFDACLAGIGGCPHAPGASGNVSTEDLAYMLRSMGVETGLDI
                     DALLALRAQVAGWLQGEALHGTLWRAGLPKTLLAA"
     gene            12794..13699
                     /locus_tag="PKV30_01785"
     CDS             12794..13699
                     /locus_tag="PKV30_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL28984.1"
                     /translation="MRDLDLTSLRLFVAVCEARSIARAAEQQAIVGSAISKRLAALED
                     TVGTPLLVRRRHGVEPTPAGDTLLEHARALLARAAQIERDMAGYAAGVRGHVRVMATQ
                     SAIAESLADDVAAFLKMPAHRDIRIDMEEAVSTQVLRGVREGSASLGICWDAADLRGL
                     HHWPWRGDHLAVVMPPSHPLARNRRLDFADTLPFEHVSMPPESAVQVMLQRAAALAGA
                     PIKYRVVAATFDAALRVVRSQLAIAILPAELSAPYVEAFGLAVTPLSDPWARRRFAIC
                     CRDEQALTPAARLLIEHLVASAQAA"
     gene            complement(13789..14772)
                     /locus_tag="PKV30_01790"
     CDS             complement(13789..14772)
                     /locus_tag="PKV30_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HRL28985.1"
                     /translation="MAFFAIAIALLLEQVRPLAADHPAATGLRRWLRLVGRNVDAGGV
                     QHGWLAWLLAVGVPTLGVIAVHWFLAWLGGWPLVLVWSVVVLYLTLGFRQFSHHFTGI
                     RDALEAGDEERARVLLARWQQVDASSVPRSEIVRHVIEYSVLAAHRHVFGVLAWFCFM
                     AVLGLGPAGAVFYRNAEYAARYWRRKSEAPDQPSSAALRSVADAAWYVIDWLPARMTA
                     LGFAIVGSFEDAIDAWRQHAARFANGNDGVILAATAGAIGVRLGGASLRPLAVDIEGQ
                     PVPNPGALAGDGLPGEAARTPHFTQVVGLVWRTVALWLLLLVLLTLAHVLG"
     gene            complement(14957..15700)
                     /locus_tag="PKV30_01795"
     CDS             complement(14957..15700)
                     /locus_tag="PKV30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRL28986.1"
                     /translation="MTSFTPLSKLPNFNPRQVPVTGVDAHLPAVPPERLTATALRQRF
                     VTPPAWQPELREEPRFADRAPAAAAVLVPLVDRPGGLSVLLTERTANLSTHSGQVAFP
                     GGKVDPEDASVVDAALREAQEEVDLPRSFVDVLGQLPVYVTGTQFIVTPVVAVVRPGF
                     VLHPNPGEVAHAFEVPLAFLMNPAHHRRHRYEWDGHVREWFSMPYVERVRVEPDAGES
                     PPRPENEVEHFIWGATAGMLRNFYRFLSA"
     gene            complement(15832..16227)
                     /gene="rplS"
                     /locus_tag="PKV30_01800"
     CDS             complement(15832..16227)
                     /gene="rplS"
                     /locus_tag="PKV30_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRL28987.1"
                     /translation="MNLIEALEKEEIERLGKNVPEFAPGDTVIVSVNVVEGSRKRVQA
                     YEGVVIAKRNRGLNSGFTVRKISSGEGVERTFQTYSPLIAKIEVKRRGDVRRAKLYYL
                     RGLSGKKARIKEKLPSRVAKQLGETATAE"
     gene            16472..18091
                     /locus_tag="PKV30_01805"
     CDS             16472..18091
                     /locus_tag="PKV30_01805"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRL28988.1"
                     /translation="MSLVRPSLARLFWVAALSATVAACGGSDDDSAPAPSPAPTPAPS
                     PAPGGNATLNGRVTSSTDGSAVAGARVTAGNASATTDANGNYSMSNAPAGERTVVRVN
                     ATGFGEGFAVESTAANTTRTANVRLQPVGGVTQGAGSGTVTATVSGSTAQVVIPANSV
                     VDAAGTPITGNYSVALTPINPAQDVTAMPGYYRVALAGGGVGQMESWGALNVTLTDAT
                     GRRLNLGSGKTATLRIPVATRSAELPASIPLFYFDESGGLWVQQGSATLHGTGAGAYY
                     EGSVSHFSYWNADRLLETVYVNGCVNNLNAQGQAAGAAANVQVTSDGIDYSGSSAIRT
                     DAAGKFRLPIKRGGKAIVAGFLDGKITNSVTAGPANADITLPDCLTLEAQASGVSIKL
                     TWGARPYDVDSHLRTPDGSHIYYASKGSLTQAPFANLDVDDTDSNGPEVVTIRRLMIG
                     TYTYGLRNYSDSFDPGMTGSPVRVELNYAGRSQVFGPPAGEVADSTDWLTLFNLTVDA
                     RCNISVTPVNRWSTSDLVSVPAPVAASYCTP"
     gene            complement(18227..>18526)
                     /locus_tag="PKV30_01810"
     CDS             complement(18227..>18526)
                     /locus_tag="PKV30_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL28989.1"
                     /translation="DEGSHQADSFNPAVDGLLDCPHYTRPEEWAGQRVPAELLSGHHA
                     QIERWRREQRLALTARLRPELVEAARRSNALTPQDERFLSDFGLQRTSGKREQLL"
BASE COUNT         3213 a         6313 c         6110 g         2890 t
ORIGIN      
        1 gcgtccatga tgttggactt gccgcagccg ttgggcccca ccacgccgac caattggccg
       61 ggcagcatga agttggtggg ttcggcgaag gacttgaagc cggagagctt gatggaattg
      121 agacgcactg gcgcagcggg ccgtcaggcc aaaaatgaag gcgaggcaaa ggcgggatga
      181 taccgtgcgt caccgcccga accgccctca gccagaggga aacgcgctgt ttcagccgcc
      241 catgcacgct tcaataaaca tagctgctgg cgcacaccag acaagcgcca gcggccgatt
      301 tcaccccaaa gtacacatca cgacgccacc acgcgccgcg ccagacttgc caagggcctc
      361 tcgatccagc tttcgagctt gcgcggcagc atcagcggtt tcagcagcat gcgcaccgcc
      421 tggtcggccg gcaggcggct gctgaccagt tgggagacgc gctggctgat ctcgtcgaag
      481 ctcttggcgc catcggcaaa catgcggccc ttgtgcgccc agacgtgtcg cagggccaca
      541 tcggcatcgc tctcgcgcag ctccagcagg cgccggtaca ccacacgcgc cacgcgcagg
      601 cgcgaggccg gttgggcggc gtgcaactgc ttgaaatacg ccagaaagtg cttgtagtgg
      661 ctgacctcgt cggcgcggat gcggcccagc atgtcggtca gcaccggctc gtcgctcaaa
      721 tcgcgcaggg tctggtaata ggtggtggtg ccggtttcga ccacgcagcg cgcggccagc
      781 tccagcgcgc ggttgggctc cagctcttcc atggtgcaca gcgggccgta ttcggcgatg
      841 aagccatcga atgcggcttg ccacggaaac tcgggccagg cggctttcac gtagcactcg
      901 aaggcgcggc cgtgctgcag ctcttcgtgc tcccagtgct cgagcagcca cgccgcgact
      961 tcggggtaat cggcgtagtg gtccgacagg ttgcgcgtgt acagatcgga gcctgattcg
     1021 atgaacgagg cgctgaccag caggtagaaa aggtcttcgc gcgcggcgat gcgggcgggg
     1081 tcgatggcgg cgaaatccag cgcctcgacg cgccagtgcg aggggcggcg ctctggcgcg
     1141 gcgtcgggtt cagtgcttgc gcgctccgcg ccagacccgg cgtgcagggg cgcgccgatg
     1201 gccacagcgg ataaagactc acggttcatc atcccggcca ttttaaggcc gtggccggcg
     1261 cctcacttgc gcggtgcgct atcaaaaagt gatggtcgaa ttcagacgcg atcgcgcaaa
     1321 cccttcacgc ccgccttctc tgcgcagtgg tcacagcaga aaaagcgccc atcgcctgcc
     1381 tccagaccgt ggccgaggat gcgcaccttg cagtgctcgc agctgggcgc cagtttttgt
     1441 gccgcgcatt cgatgctgtc gaaggtgtag gtctggccgg acgcggtcac ggtgaaggcc
     1501 ttgtcgtaca cgttgccgca ggtggagcag gcttggctca tgggtcgtct ccttggtgtc
     1561 ggtctcaaaa cttcacctta aaaccccgcg acacgccggc aagtaggacg atggcgcctg
     1621 ccaacgtgcg cggcgccgat aatcgcccgc catgaacccc ctgctttcgc gcctgcagcc
     1681 ctaccccttc gagcggctgc gcaagttgtt cgagggcgtg acgcccaacc ccgcctaccg
     1741 cccgatcagc ctggggattg gcgagcccaa gcacgccacg ccgcaattca ttcaggacgc
     1801 gctggcgggc gatctggcaa cgggtttgtc tggctacccg cccaccgccg gctcgcctgc
     1861 gctgcgcgag gcatgcgcgg gctggctgca gcgccgctac ggcgtgacgg tggatgccgc
     1921 cacgcagatg ctgccggtgc tcggctcgcg cgaggcgctg tttgcgctgg cgcaagtggt
     1981 ggttgatccg acgaagccga acgccacggt ggtctgcccc aaccccttct atcaaatcta
     2041 cgaaggcgcg gcgctgctgg cgggcgctga agccttctac gtggcgagcg acccggcgcg
     2101 caacttcgcg ccgcgctggg atcaggtgcc ggccgaggtg tgggcgcgca cgcaactcgt
     2161 ctacgtctgc tcgcccggca acccgaccgg cgcggtgatg ccgctggatg agtggaagct
     2221 gctgttcgag ctgagcgacc gccacggctt cgtcatcgcc tcggacgagt gctacagcga
     2281 gatctatttc cgtgacgagc cgccgctggg cgggctggaa gcggcggcca agctcggccg
     2341 aaccgatttc aagaacctgg tgatgctgac cagcctgtcc aagcgcagca acgtgccggg
     2401 catgcgttcg ggcttcgtgg cgggcgatgc ggcgatcatg aaggcgctgc tgctctaccg
     2461 cacctaccac ggcagcgcca tgagcccggt ggtgcagacg gccagcatcg ccgcctgggg
     2521 cgacgaggcg catgtggagg acaaccgccg cctgtaccgc gaaaagttcg ccgccgtcac
     2581 accggtgctc gcccgcgtga tggacgtgaa gctgcccgat gccgcctttt atctgtgggc
     2641 gggcgtccca gcggcgtgga atagttccga caccgacttc gcccgcgaac tgctcgctca
     2701 atacaatgta acggttttgc ccggcagtta cctggcgcga gaagccaatg gcgccaaccc
     2761 aggccaaggc cgcgtgcgca tggcgctggt ggccgacact gccgaatgcg tcgaagcggc
     2821 cgagcgcatc gtccaattca tccaatccaa atcctgaaaa gcaccatgac ccaacaactg
     2881 cagcagatca tcgacgccgc gtgggaagac cgcgccaacc tgtcgcccac gaaggcaccc
     2941 aaggaagtgg ccgacgcggt ggagcacgtc atcagcgaac tgaacgccgg ccgcctgcgc
     3001 gtggccacgc gcgaatcggt cggcaaatgg acggtgcacc agtggatcaa gaaagcggtg
     3061 ctgctctcct tccgcctgaa ggacaacgac ttcatccgcg ccggcgacct gggctttttc
     3121 gacaaggtgc agaccaagtt tgcccacctc gatgagtccg ccatgcgcga aagcggcgtg
     3181 cgcgtggtgc cgcccgccgt ggcgcggcgc ggcagcttca ttggcaaggg cgcggtgctg
     3241 atgcccagct acgtcaacat tggcgcttat gtggacgaag gcaccatggt cgacacctgg
     3301 gccaccgtgg gcagctgcgc gcagatcggc aagaacgtgc acctgtcagg cggcgtcggc
     3361 attggcggcg tgctggagcc gctgcaggcg ggccccacca tcattgaaga caactgcttc
     3421 atcggcgcgc gcagcgaggt ggtcgaaggc gtggtggtcg aagaaaactc ggtgctcggc
     3481 atgggcgtgt acatcggcca gagcacgccg atcttcaacc gcgaaaccgg cgagatgagc
     3541 tacggccgcg tgccctcagg cagcgtggtc atcagcggcg gcctgcccaa gaagtgcgcc
     3601 gacggcacgc cctacaacac ctacgccgcc atcatcgtca agacggtgga cgcgcaaacg
     3661 cgctccaaga ccagcatcaa cgacctgctg cggccctgac gccagatcag atcagaaaag
     3721 cccgccagcg cttgaccagc ctgcgcaagc agatacgatt ttaatagcac cacgcagcac
     3781 catggccact tcagtccccg cgcccaccaa catgatggag cgcatcctgc atctgatggc
     3841 cgagaaaaag gcgtcggacg tgtacctgtc ggcgcacgcg ccggccacca tcaagatcaa
     3901 cggccagtgc atgccgatca acagccaggt gatgccgccc gaagcggtga tgaacctgtt
     3961 gatggacgtg gtgccgccca accgcatcga agaactgaag gaaaccggcg agctcaacat
     4021 ggccatcccc atgtacggcg tcggcaactt ccgcctctcg gccatgcgcc agcgcggtac
     4081 ttacgcggcg gtgatccgtt tcatcagccc tgaaatcccc gacctggatt cgctgcacct
     4141 gccctccatc ctcaagcacc tgatcatgga aaagcgcggg ctgatcctga tggtgggcgc
     4201 cacgggtgcg ggcaaatcga ccacgctggc ctcgatgctg gaccaccgca acaagaacgc
     4261 cagcgggcac atcctgacca tcgaagaccc gatcgagttc atcttcacca accgcaagtc
     4321 gctggtgaat cagcgcgagg tgggcagcga caccgccgtg ctgcaaactg gcctgaagaa
     4381 cgcgctgcgc caggcgcccg acgtgatttt gatcggcgaa atccgcgacc gcgacaccat
     4441 gagcgccgcc attgcctacg cgcaatcggg ccacctgtgc ctggccacgc tgcacgccaa
     4501 caacagctac caggcgctca accgcgtgct gtcgttctac ccggccgagg tgcggcccac
     4561 gctgctgggc gacctgtcgg cggcgctcaa gtcggtggtg tcgcagcgcc tgatccgcac
     4621 ggtggatggc gggcgcacgc cggcggtcga ggtcatgctc aacaccaagc tgatccagga
     4681 tttgatcgag aaaagcgatt tctccggcgt caaggaagcc atggacaacg ccatggccga
     4741 aggctcgcag actttcgagc aagacctggc gcgcctgatc ctcgaaggca agatcgaccg
     4801 caaggaaggc ctggccaacg ccgattcgcc caccaacctg atgtggcgcc tgcaaaacga
     4861 ctcaggacgc ctcaacccca acaagccgca ggaggaagaa cggccaagcg acgagccctc
     4921 gttcaccgag atcacgctgg acgtcaagcc cagcggctac cgcaccactg gctttggcaa
     4981 gaccggcttt ggcaacagcg gcttccgcga ttcgggcttt caggattcga agatgggcgg
     5041 ctattccgat tcgcgcatcg gcggcgcatg atccgcacgg gttcggcgat gggcgtgaat
     5101 gcccaagcac cttggccctt cacgctcaac gtcccatgct gacctctcga catgcccgcc
     5161 actcgcctgc ttgctgaaca actcatcgcc cgcccctcgg tcacgccgct ggacgccggc
     5221 tgcatcgagc tgatcgccgc gcgcctggcg ccgctgggct ttgtcaacga gatcatcgac
     5281 agcgggccgg acacattccg cgttcgcaat ttgtggtcta aaaggcctgc agcgcacgcg
     5341 ggacaagcgc gagcagctat caaaaccata gttttcgcgg gccacacgga tgtggtgccc
     5401 accggcccgc tcgagcagtg gagcagcgat ccgttcgcgc ccaccgagcg cgacggcaag
     5461 ctctacggcc gcggcgcgag cgacatgaaa acgtcgatcg ccgccttcgt tgtcgcggtg
     5521 gaggaactca tcgtcgcgca gccgaacgca ccactccaca tcgccttcct gctcaccagc
     5581 gacgaggaag gcccggccgt cgatggcacc gttgtcgtct gcaacgtgct caagtcgcgc
     5641 ggcgaacggc tggactactg catcgtcggc gaaccgacca gcgtggaacg caccggcgac
     5701 atgatcaaga acggccgccg cggcactctc tcgggcaagc tcacggtgaa ggggattcag
     5761 gggcacatcg cctacccgca cctggcgcgc aacccgattc acgatctggc gcccgcgttt
     5821 gccgaattgg tcgccatcga gtgggatcgc ggcaacgcgt tctttccgcc caccagctgg
     5881 caggtcagca acatccacgg cggcaccggc gccagcaaca tcattccggg cagcgtggtg
     5941 gtcgacttca acttccgctt ctcgaccgaa tccacgcccg aatcgctgca gcagcgcgtc
     6001 gaagcggtgc tgcaaaagca tggactggag tacgaactga aatggacgct cggcggcctg
     6061 ccctttctca ccacgccggg cgatctggtc accgccgtgc aggccgccat ccacgccgaa
     6121 acggggctgg agaccgagtt gtccaccacc ggcggcacca gcgacggccg cttcatcgcg
     6181 caaatctgcc cgcaggtgat cgagctcggt ccccccaacg ccagcatcca caagatcgac
     6241 gagcacatcg cgctcgccga catcgagccg ctgaagaata tctaccgccg ggtgcttgaa
     6301 aacctggccg ctgcatgacg ctcgccgagc tggtggccga caccgaacgc caactgatcg
     6361 aagccggcgt cagctttggc cacggcacca ccaacgccca cgacgaagcc gcctggctgg
     6421 tgctgcacgc gctcggcctg ccgctcgata ccgacatcga tggatcagaa ggaaaacagc
     6481 ctgcagcgcc cgacggacaa gcgcgaacag ctacgctttt gaaagcacgc atcgacacgc
     6541 gccagccgct cgcctacctg ctgaaagagg cctggctgca aggcgtgccc ttctacgtcg
     6601 atgagcgcgc catcgtgccg cgcagcctga ttgccgaagt gctggcagac ggcagcatcg
     6661 acccgtggct gtcggatcag acgcaccgcg tgctcgacct gtgcaccggc aacggcagcc
     6721 tggccgtgct cgcggcgctg gcgtggcccg aggtgcaagt cgacgccgcc gacctgagcg
     6781 tcgaagcgct ggccgtcgcg cgcatcaacg tcgaaaaaca tggcttgaag gatcgcatca
     6841 agccgatcga aagcgatggc ctgcacaact gccccggacc gtacgacctg atcctttgca
     6901 acccgcccta cgtcaacgtc gccagcatgg ccgccctgcc cgccgaattc cgcgccgaac
     6961 ccgcgctggc gctggccggc ggcgccgacg gcatggactt catccgcgcg ctgctgcgcg
     7021 atgcgcccac gcgcatgaac gagcgtgccg tgctggtgct ggaaatcggc catgaacgca
     7081 cgcacttcga gaccgccttt ccccgactga accccgtgtg gctcgacacc agcgctggcg
     7141 gcgatcaggt gttgctgctc acatgcgagg cgctggcacc atgattcagc tgcaaaacct
     7201 gacgctgcgc cgcggcgcca aggtgctgct cgacggcgcc agcatcacca tccaccccgg
     7261 cgagaaagtc gggctggtgg gccgcaacgg cgccggcaag tcgacgctgt tcgcgttgct
     7321 gaacggctcg ctgcatgagg acggcggcga cttctcgatg cccgccagct ggcagatcgc
     7381 gcaggtggcg cagcacatgc ccgagaccac cgaaagcgcc accgatttcg tgctttcagg
     7441 cgacgagcgc ttgtcccgct tgcgcgagca gctctcggaa gccgagcaat cgggcgacgg
     7501 catgctgatc gcgcacgcgc acagcgacct gcatgacgcc ggcgcgcacg atgccacggc
     7561 gcgcgcgcag gcgctgatcc tgggcctcgg cttcaaggtc gaggagttgc atcgccccgt
     7621 caacagcttc tcgggcggct ggcgcatgcg cctgcaactg gcgcgcgcgc tgatggcgcc
     7681 gtccgatctg ctgctgctgg acgagccgac caaccacctc gacctcgacg cgctggtgtg
     7741 gctggaaggc tggctggcgc gctaccccgg cacgctgatc gtcatcagcc acgaccgcga
     7801 ttttctcgat gcggtgacgc gcgtcacgct gcacatcgag aacggccgtc tggcgcgcta
     7861 cggcggcaac tacagcgcct tcgaggaatt gcgcgcgcag caactcagcc tgcagcaggc
     7921 ggcgttttcc aagcagcaag acaagatcgc gcacctgcag aaattcatcg accgcttcaa
     7981 ggccaaggcg accaaggcca agcaggccca aagccgcgtg aaggcgctgg agcgcatgga
     8041 gaagatcgcg ccggtgctgg ccgacgccga gttccagttc gagttcaagg aacccgccag
     8101 cctgcccaac ccgatgctgg ccgtgcgcga tggcgtgttt gggtaccgca gcgacggcac
     8161 gacaaaatca gaagctgctg gcgctgatct aacaagcgct ggcggctcat cggatgccaa
     8221 gcccgcgctc agtgactacg ccaccacggc cacgctgaac gcgcagctgg ccgcgcgcgg
     8281 cgaattgccg cccgagccgc aggtcggcga ccgcgtcatc ctgcgcggcg tcagccgcac
     8341 cgtgttggcg ggccagcgca tcggcatcct gggcgccaac ggtcagggca aatcgacctt
     8401 cgtcaagacc atcgcacgca cgctgccgct gctgggcggc acggtgaccg aaggcaaagg
     8461 cctggcgatc ggctacttcg cgcagcagga gctggacgtg ctgcacgccg acagcacgcc
     8521 gctggaacac atggtgcggc tggcgcgcga caccggccgc gcccacaccg aggaggcgcg
     8581 cgagcagcaa ctgcgcaact tcctcggcag tttcagcttt ccgggcgaga tggtgacgca
     8641 gcgagtcggc cagttctcag gcggcgaaaa ggcgcggctg gtgctggcga tgatcgtctg
     8701 gcagcgcccc aacctgctgc tgctcgacga gccgaccaac cacctcgacc tggccacgcg
     8761 cgaggcgctg gcggtggcct tgaacgagtt tgaaggcacg ctgatgctgg tcagccacga
     8821 ccgcgcgctg ctgcgcagcg tgtgcgacga gttctggttg gtcgcgcgcg gcggcgtggc
     8881 gccgttcgat ggtgatctgg aggactacca gcgctatctg ttggacgaag ccaagcgcct
     8941 gcgcgcggcg gctgccgatc aggtagctgc ggattcgata gctgtcgccg caatcccaga
     9001 aagcgccgca gcccaaaagc ctttgcaaga accgctcaac ccggtcaacc ccgccgagca
     9061 gcgccggctt gatgcccagc gccgccagca actggccgag aagacgcgac cgctcaagcg
     9121 cgcgctggaa aaaaccgagc aacgcatgca ggcgctgggc gccgagcagg caacgatcga
     9181 gcagcgcctg gccaccgcgc tgccccccgc cgacatcgcc gaagccggcc gccgcctgaa
     9241 ggcagtgggc gacgaactgg ccacgctgga agacgagtgg ctgcgcctgt cgggcgagat
     9301 cgaggcgatc gagtagcccg ccgctggcgc gctcgctact gcggcgtcac gcccgctttc
     9361 ttgaccagcg ccgcaaagcg cgcgccctcg gccttgaagg tggccagcgc ctgctcaggc
     9421 gtgccgatct ggatcacgtt gccctgcttg gccatggcct ctttcacgtc gggcgcatcg
     9481 aaggcggcgg ccacggcgtc gtgcacgcgc ttgacctgcg ctgccggcag acccttaggg
     9541 ccgagcacgg caaaccagcc gtccaccacg tagccaggca agccctgctc ggcaaacgta
     9601 ggcacgtcgg gcgcagcggc caggcgctcg ccgctggagg tgccgatgat gcgcagcgca
     9661 cccgacttga cgtgctgttg cgccgtcacc aagcccagca cgccgaaatc gacctggccg
     9721 ccgatcaggt cggtcaccat ctggccgacg cccttgtagg ggatgtggcg cgccttgacg
     9781 cctgcctcgt cgacaaacat ttcagccgcc aggtgcagga tggtgccggt gccgccgctc
     9841 gcaaagttgt agccgtcggg cttggccttg agcagcgcga cgaactcctt gctgttggtg
     9901 gccggcacct tggtcgggtt gaccacaagc accagcggcg tgatgccgac caccgcaatc
     9961 ggcgtgaagt cttccggcat cttgaacggc acgcttttca gcacgtgcgg caggatcacg
    10021 acgttgttgc tgacgaagga cagcgtgtgc ccatcgggcg ccgcgcggct gagcgcttgc
    10081 aggccgatca cgccgctggc gccgggctgg ttgtcgatga cgaccggctg gccgagcgcc
    10141 ttggccagcg ccggttgcgc ggcgcgcacg atggtgtcca cgcccgagcc ggccgcattc
    10201 ggcaggatga atttgacggc gccgcccgca ggggcttgcg ccagcgcagg ggcgttggcg
    10261 gccaggacca gtgcagcgaa tgtggcgagt gcagcgcggc gcgtgctgaa gcgatgtgac
    10321 gcgggcatgg gtggtgtctc cgttttagga tttttcagat gattcatggc tcaagcgacc
    10381 gcgtggccag cgcggagcgc ttcgatttca gcagcgtcat agcccagctg caccagcagc
    10441 gccgccgtgt cgcgccccat ggtgggcggt tgcgtgccca ggcccaggcg ctcgccgccc
    10501 agggtgacgg gcagcagcgt ggtctgggcc ggctggccgg cgcgcgcgcc gtcggtgagg
    10561 gtgatgtcgg acagaccacc ggtcgccagc agatgcgggt cgtcgtacag ctcttcgggc
    10621 ttgcggatgg gcgcaaaggg caagccgact ttttcaaaca gcgccgccag ctcggccgcg
    10681 ctgcgcttgg ccaggcgttc gcgcagcacg ggcatcaaag tcgggcgggc gcgcacgcgc
    10741 tggttgttgg tggcgtggtc gggcaggtcg cgcagatcgg ccaagcccag cgcgtcgcag
    10801 aaggtgcgcc attgcgcatc gctgacggcg gccagaaaaa tctgctcgcc atccttcacc
    10861 gtgaacacgt cgtacacggc ccaggcgctg atgcggtcgg gcatgggcgc ggccggctcg
    10921 ccggtgatgg cgtactgcag catgtgctgg ccgaccagga acacgttgtt ctcgaacagc
    10981 gcgctttgca cttcctgccc cttgccggta atgccgcgct ggatgatggc gcccaaagcg
    11041 ccgatggcgc cgaacatgcc gcccatgatg tcgttcacgc tggtgccggc gcgcagcggg
    11101 tcgcccgggc ggccggtcat gtaggccagg ccgcccatca tctgcaccac ctcgtcaagc
    11161 gccgtgcggt gctcgtaggg gccgggcaga aagcccttgc agctgacgta gatgaggcgc
    11221 tcgttctgcg ccgacaacgc ggcgtagtcg aggccgtact tggtcatggt gccgggcttg
    11281 aagttctcgg ccaccacgtc ggccgacagc gccagcttgc gcgccacggc ggcgccctcg
    11341 ggcgtgtgca ggtcgatggc gatgcttttc ttgccgcggt tgaacatggg gaaaaagccc
    11401 gcgcccgcgc ccagcaggtg gcgcgtgcgg tcgccatcga tgggctcgac cttgatgacc
    11461 tcagccccca tgtcggccag caccaggccg caggttggac ccatgaccat gtgggtgaat
    11521 tcgaccacgc gcaggccttc cagaggccgc gggcgcttgt tggtattggt ttgattgctc
    11581 atgtcttgat agtgctccag atcaggcggc cagcagcgtc ttgggtaagc cggcgcgcca
    11641 cagcgtgcca tgcagcgcct cgccctgcaa ccagccggcg acttgcgcgc gcagcgcgag
    11701 cagtgcgtcg atgtccaggc cggtctcgac gcccatgctg cgcagcatgt aggccaggtc
    11761 ttcggtgctg acgttgccgc tggcgcccgg tgcgtgcggg cagccgccga tgccggccag
    11821 gcaggcatcg aaacgcgcca cgccggtctg cagggccgcg tagacgttgg ccaggcccaa
    11881 gccgcgcgtg tcgtgaaaat gcccggccca cagcttgtcg cccgccacct tgcgcgccgc
    11941 ttcgaacagg ctttgcacgg cggccgggtc ggcgtagccc acggtgtcgg ccaggctgac
    12001 gcggtcgcag ccggcatcga gcagcgcctg cagcaggcgc agcacttcgc tgtgcttgac
    12061 ctcgccctgg atcgtgcagc caaaggcggt gctcatgccg ccttccaaca gcgttttcga
    12121 gccggcggcg tcgcgcgcgg cgcggatgcg ggcgacgtcc tgcaccacct cgtccggcgt
    12181 cttgcgcagg ttggccaggc tgtgcgcgtg gctggccgac agcggcacca gcatcaggtc
    12241 ggcaccgctt tcgatggcac tttcagcgcc gcgcatgttg ggcaccagca ccgagacaaa
    12301 caggcccggc agcgtcttgg caaacgcgac cagctcggcc gtgtcggcca gttgcggcag
    12361 gtatttggcg ggcacgaagg aaccgacttc gatctcgcgc tggccggccg cgtgcgcgcc
    12421 acgcagccat tgttttttct gctcggtcgg cagcgtgcgg gcgatggcct gcagtccgtc
    12481 gcgcaggccg acttcacgaa tcaccgcgcg gcgcggcaga gtggatggcg tgctcatgcc
    12541 ttgtctcctt tgtgttttgt tcgtggcgag tttagatatt ccacggaaga tcgccaacgg
    12601 tactttggaa gctctatgat tcctcaatgg aaaacctgac ggctgtctct ccctctttgg
    12661 cggctgcgca gcaagccgct tcaaaaggcc gcgaagcagg ccacgccgga acgccgtggc
    12721 cggggtttcc ccggccgcca gcgttgtccc cccgctgggg gggaaggcgc gcagcgccat
    12781 aggggggagc gcagtgcgcg accttgacct gaccagcctg cgcctctttg tcgccgtgtg
    12841 cgaggcgcgc agcattgcgc gcgccgccga gcagcaagcc atcgtcggct cggccatcag
    12901 caagcggctg gcggcgctgg aagacacggt gggcacgccg cttttggtgc ggcgccgcca
    12961 tggcgtcgag cccacgccgg cgggcgacac gctgctggag cacgcgcgcg ccctgctcgc
    13021 ccgcgcggcg cagatcgagc gcgacatggc cggctacgcg gccggcgtgc gcggccacgt
    13081 gcgcgtgatg gccacgcaat cggccatcgc cgaatcgctg gccgatgacg tggcggcctt
    13141 cctgaaaatg ccggcgcacc gcgacatccg catcgacatg gaagaagccg tcagcaccca
    13201 ggtgctgcgc ggcgtgcgcg agggcagcgc atcgctcggc atttgctggg acgcggccga
    13261 cctgcgcggg ctgcaccact ggccgtggcg tggcgaccat ctggcggtgg tgatgccgcc
    13321 cagccatccg ctggcgcgca atcggcggct ggactttgcc gacaccctgc ctttcgagca
    13381 cgtcagcatg ccgcccgaaa gcgccgtgca ggtgatgctg cagcgcgccg ccgcgttggc
    13441 cggggcgccg atcaagtacc gcgtggtggc ggccacgttt gacgccgcgc tgcgcgtggt
    13501 gcgctcgcag ctggcgattg ccatcctgcc ggccgagttg tcggcgccgt atgtcgaggc
    13561 gtttggcctg gccgtgacgc cgctcagcga cccgtgggcg cggcgccgct tcgccatctg
    13621 ctgccgcgac gagcaggcgt tgacgccggc ggcgcggctg ctgatcgaac acctggtggc
    13681 ctcggcgcag gccgcgtaac gccgtcgccg caccagacgg ttactacaaa caagatagct
    13741 gctttcgctt gatgggcaag cgccagaggc cgttttggct tgaaattatc agcccagcac
    13801 gtgggccagc gtcagcaaca cgagcagcaa aagccacagg gcgaccgtgc gccacaccag
    13861 ccccaccacc tgcgtgaagt gcggcgtgcg cgccgcctcg cccggcaagc cgtcgccggc
    13921 cagcgcgccg gggttgggca cgggctggcc ttcgatatcg accgccagcg gccgcagcga
    13981 ggcgccaccc agccgcacgc cgatggcacc ggcggtagcg gccaggatca cgccgtcgtt
    14041 gccgttggca aagcgcgccg cgtgctggcg ccaggcgtcg atggcgtcct cgaagctgcc
    14101 gacaatggca aagccgagcg ccgtcatgcg cgccggcagc cagtcgatga cgtaccaggc
    14161 cgcatcggcc acgctgcgca gcgcggcgct gctgggctgg tcgggcgcct cggatttgcg
    14221 gcgccagtag cgcgccgcgt attcggcgtt gcggtaaaat accgccccgg ccggccccag
    14281 ccccagcacg gccatgaaac agaaccaggc cagcacgcca aacacgtggc ggtgcgcggc
    14341 cagcaccgaa tattcaatga cgtggcgcac gatctcgctg cgcggcacgc tgctggcatc
    14401 cacctgctgc cagcgcgcca gcagcacgcg ggcacgctct tcatcacccg cttccagcgc
    14461 gtcgcggatg ccggtgaagt ggtggctgaa ctgccggaag ccgagcgtca ggtacagcac
    14521 caccacgctc cagaccagca ccagcggcca gccgcccagc caggccagga accagtgcac
    14581 ggcgatcacg cccagcgtgg gcacgcccac cgccagcagc cacgccagcc agccgtgctg
    14641 cacgccaccc gcatcgacat tgcggccgac caggcgcagc cagcggcgca ggccggtggc
    14701 ggccgggtgg tcagccgcca gcgggcgcac ctgctctagc aggagtgcta tcgcaattgc
    14761 aaaaaaggcc ataactggat gatagttgtc cgcggagccg cccgccatcc aaagcggcaa
    14821 acttagaccc atcgaatggc cgcggagcag gccaccccag cgaacgccgt ggccggacct
    14881 gcgccggcca ctggcgttgt cccctcccga cggagagggg ggaagccgcg tcagcggctc
    14941 aggggggtgc tctctttcaa gccgacaaga atctgtagaa gttacgcaac atgccggcgg
    15001 tggcgcccca gatgaagtgc tcgacttcgt tctcgggccg gggtggcgat tcgccggcgt
    15061 cgggctcgac gcgcacgcgt tcgacgtagg gcatcgagaa ccactcgcgc acgtggccgt
    15121 cccattcata gcggtggcgg cggtggtgcg cgggattcat caggaaggcc agcggcactt
    15181 cgaacgcgtg cgccacttcg cccgggttgg ggtgcagcac aaagcccggg cgcaccaccg
    15241 ccaccacggg cgtgacgatg aattgcgtgc cggtcacgta caccgggagc tggcccagta
    15301 cgtcgacaaa gctgcgcggc agatcgacct cttcctgagc ctcgcgcagc gctgcgtcga
    15361 ccacgctggc gtcttcaggg tccaccttgc cgcccggaaa ggcgacctgc cccgagtgcg
    15421 tcgagaggtt ggccgtgcgc tcggtcagca gcaccgacag accgcccggc cgatcaacca
    15481 gcggtaccag cacggcggcg gcagccggtg cgcggtcggc aaagcgcggc tcctcgcgca
    15541 actcgggctg ccacgcgggc ggggtgacaa agcgctggcg cagggcggtg gcggtcaggc
    15601 gttcgggcgg cacggcgggc aggtgcgcgt cgacccccgt caccggcacc tggcgcgggt
    15661 tgaagttggg cagcttggac aggggggtga aagaagtcat ggcagacaaa aggctaagcg
    15721 ccattgtgcg caaacggtgc gcaaacctcg cctggcaaga aaaaaccgcc tgagccaaag
    15781 gcagcaggcg gtcggtctgg tgcacaaccc gcaaacgggt tggcgccgga tttactcggc
    15841 ggtggcggtc tcgcccagtt gcttggccac gcggctcggc agcttttcct tgatgcgggc
    15901 cttcttgccc gacaggccgc gcaggtagta cagcttggca cggcgcacgt caccgcggcg
    15961 cttgacttcg atcttggcga tcagcgggct gtaggtctgg aaggtgcgtt ccacgccttc
    16021 gccgctggag atcttgcgca cggtgaagcc gctgttcagg ccgcgattgc gcttggcaat
    16081 caccacgcct tcgtaggctt gcacgcgctt gcggctgcct tcgacgacgt tgacggacac
    16141 gatcacggtg tcgccaggag cgaactcagg cacgttcttg cccagacgct cgatttcctc
    16201 tttttcgagg gcttcgatca ggttcatggt ttctctcgtc tcgatcgtgc gcacgctacg
    16261 ctgggggcgg ctgggcttga aaagcctttt aagtgccgcg gcgggcagag gatcgaaaag
    16321 ccctccattc tacttcgatt tgggcgtcgt gccatctctt gctgttcgac agcagccgcg
    16381 acaccacccg acttcggtag ggtatgcaaa caaaaggata cctcctagag ttcgtgactt
    16441 atttcacaca cgaatgagga gttgatccgc aatgagtctt gtccgtccca gccttgcgcg
    16501 ccttttctgg gtggccgcgc tgtcggccac cgtcgccgcc tgcggtggca gcgacgatga
    16561 cagcgccccc gcgccgtcgc ccgcccccac gcccgcccca tccccagcgc ccggtggcaa
    16621 cgccaccctc aacggccgcg tcaccagcag caccgacggc agcgccgtgg ccggcgccag
    16681 ggtcaccgcg ggcaatgcca gcgccacgac ggacgccaac ggcaactatt cgatgagcaa
    16741 cgcccccgcg ggcgagcgca ccgtggtgcg cgtcaacgcc acgggctttg gcgaaggctt
    16801 cgcggtcgaa tccaccgccg ccaacaccac gcgcaccgcc aacgtccggc tgcagccggt
    16861 gggcggcgtg acgcagggcg cgggctcggg cacggtgacc gccaccgtga gcggctcgac
    16921 ggcgcaggtc gtcatccccg ccaacagcgt ggtcgatgcg gccggcacgc ccatcaccgg
    16981 caactactcg gtcgcgctga cgcccatcaa cccggcgcag gatgtgaccg ccatgcccgg
    17041 ctactaccgc gtggcgctgg ccggcggcgg tgtcggccag atggaaagct ggggcgcgct
    17101 caacgtcacg cttaccgatg ccaccggccg gcgcctgaac ctgggcagcg gcaagaccgc
    17161 cacgctgcgc atccccgtgg ccacgcgcag cgccgaactg ccggccagca ttccgttgtt
    17221 ctatttcgac gagagcggcg gcctgtgggt tcagcaaggt agcgccacgc tgcacggcac
    17281 gggtgccggc gcctattacg agggcagcgt gagccatttc agctactgga acgccgaccg
    17341 cctgctggag acggtttacg tcaacggctg cgtgaacaac ctcaacgcgc aaggccaggc
    17401 cgccggggcc gccgccaacg tgcaggtgac ctccgacggc atcgactatt cgggctcatc
    17461 ggccatccgc accgatgccg ccggcaagtt ccgcctgccg atcaagcgcg gcggcaaggc
    17521 catcgtggcg ggtttcctgg acggcaagat caccaactcg gtcaccgccg gtcccgccaa
    17581 cgccgacatc accctgccgg actgcctgac gctcgaggcg caagccagcg gtgtcagcat
    17641 caagctgacc tggggcgcgc ggccgtacga cgtcgattcg cacctgcgca cgcccgatgg
    17701 ctcgcacatc tactacgcgt ccaaggggtc gctgacgcaa gcgccgtttg ccaacctgga
    17761 cgtggacgac accgacagca acggccccga ggtggtgacc atccgccgcc tgatgattgg
    17821 cacctacacc tacggcctgc gcaactacag cgacagcttc gaccccggca tgacgggctc
    17881 gccggttcgc gtggaactga actacgccgg ccgctcgcag gtgttcggcc cgccggccgg
    17941 cgaagtggcg gacagcaccg actggctgac gctgttcaac ctgacggtcg atgcgcgttg
    18001 caacatcagc gtgacgccgg tcaaccgctg gtccacttcg gatctggtct cggtcccggc
    18061 gcccgtggcg gccagctact gcacgccgta aacagtgtgt gacggcgcag cgcccaaggg
    18121 cgctgcttct ttacccggca atctggtcac gtggcctatt gccgggtttt ttcatgggcg
    18181 tttgcgtgcg cgacggcgcg acaggttccg cgtcgattcg ctacatttac aggagctgct
    18241 cgcgcttgcc agatgtgcgc tggaggccaa aatcgcttaa aaaccgctcg tcctgcggcg
    18301 tcaaggcatt cgagcggcgc gccgcctcga ccagttccgg ccgcaggcgc gccgtcagcg
    18361 ccaggcgctg ctcgcgccgc cagcgctcga tctgcgcgtg gtggccgctc aacagttcgg
    18421 ccggcacgcg ctggccggcc cactcctcgg gccgcgtgta gtgcgggcaa tccagcaggc
    18481 catcgaccgc cgggttgaag ctgtcggcct ggtggctgcc ttcatc
//
LOCUS       DAOMVO010000016        17583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_622_length_17583_cov_13.920641, whole genome shotgun sequence.
ACCESSION   DAOMVO010000016 DAOMVO010000000
VERSION     DAOMVO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17583
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_17583_cov_13.920641"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..90)
                     /locus_tag="PKV30_01815"
     tRNA            complement(1..90)
                     /locus_tag="PKV30_01815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:cga)
     gene            complement(135..1445)
                     /gene="serS"
                     /locus_tag="PKV30_01820"
     CDS             complement(135..1445)
                     /gene="serS"
                     /locus_tag="PKV30_01820"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRL28990.1"
                     /translation="MLDPILLRKDLGAVLARLATRKSPQPFLDEASFSALESERKTIQ
                     TRTEELQAQRNSASKQIGQRKAKGESADDLMAQVASIKDELDASAARLDVIQHEMATL
                     LLAVPNLPHESVPVGADEHANVEVRRWAPHGLAPRAFDFPVRDHVELGEPLGLDFETG
                     VKLAGTRFNFMRGPIARLHRALAHFMLDLQTTQHGYTECYTPYIVNADTLRGTGQLPK
                     FEADLFAAKKGGQEGEGESLYLIPTSEVSLTNLVRGEIVDESELPIMLTAHTPCFRSE
                     AGSAGRDTRGLIRQHQFDKVEMVQITHPEKSYEALEAMVGHAEAVLQKLELPYRVVLL
                     CTGDMGFGASKTYDLEVWVPAQDTYREISSVSNCEAFQARRMQARFKNAAGKNELVHT
                     LNGSGLAVGRALVAVLENHQQADGSIAIPAALRPYLGGLEVLRP"
     gene            complement(1571..2539)
                     /gene="ispH"
                     /locus_tag="PKV30_01825"
                     /gene_synonym="lytB"
     CDS             complement(1571..2539)
                     /gene="ispH"
                     /locus_tag="PKV30_01825"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRL28991.1"
                     /translation="MNKAEILLAEPRGFCAGVDRAIEIVERAIAKFGAPIYVRHEIVH
                     NTYVVNDLKAKGAIFIEALEDVPVGATLVFSAHGVPKSVEREAESRGFRVFDATCPLV
                     SKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARVQPAQTDK
                     LAVVTQTTLSVDDAAEISAAVKARFPQVREPKQQDICYATQNRQDAVKLLSSEVDVVI
                     VVGSPTSSNSNRLREVARKLGVDAYMVDGADELRAEWIEGKSRIGLTAGASAPDILVR
                     QVIERLRALGATAVRKLDGIEETVKFPLPKGLKLDQQGESQLGHMS"
     gene            complement(2539..2982)
                     /locus_tag="PKV30_01830"
     CDS             complement(2539..2982)
                     /locus_tag="PKV30_01830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRL28992.1"
                     /translation="MTTVELGSFLTLHYRLSGPQGDVINTFGGKPATLSLGAGELSPA
                     VEDRLIGLAEGAHDTFELPAGAAFGERNPDMVQWLARKELDSLGDPNERYAIGDVIQL
                     PTPDGQGQFAAVVQAVRDDGALQLDFNHPLAGQPVTFEVELIGVL"
     gene            3039..3716
                     /gene="radC"
                     /locus_tag="PKV30_01835"
     CDS             3039..3716
                     /gene="radC"
                     /locus_tag="PKV30_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRL28993.1"
                     /translation="MALKDLPPDARPREKLLARGAGALSDAELLALLLRTGTAGRGVL
                     QMAQELLAQFEGVAGLLHASADDLKRFKGLGGSAKRAELVAVLELARRALAEQLKERT
                     ALNSAQAVKHYVQLHLGARPHEVFAALFLDSQHRLIALEELFRGTLSQTSVYPREVVI
                     RALHHHASGVILAHNHPSGGVQPSRADESLTQTLKAALALVDVRVVDHVIVAPGAALS
                     MAEKGLV"
     gene            3713..4360
                     /locus_tag="PKV30_01840"
     CDS             3713..4360
                     /locus_tag="PKV30_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRL28994.1"
                     /translation="MKVKSLQDLKALQKQLAETQARAAKEAAVRAAAEQKARAEQNLF
                     ARAIGATQPLRAHGRAVHAPAPVPPEPLQRQRDEQAVLREAISDEFDASTLLQVDEHL
                     SFRRPGIGPDVLPKLRRGHWAVQRQIDLHGLRTEEAREALGQFVRDAQRAGVRCVRVV
                     HGKGLGSPGKTPVLKAKVHGWLIQKKEVLAFVQAKPLEGGAGALLVLLASAQSMG"
     gene            complement(4374..5948)
                     /locus_tag="PKV30_01845"
     CDS             complement(4374..5948)
                     /locus_tag="PKV30_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL28995.1"
                     /translation="MPASRPQSTDLPAERAVPARATAWAVLAWVVAWVMLRLSLSEAL
                     KWDYGEQMLWSQQLAWGYGPQPPLYTWLQWGANELLGPSVLALALLKGVLMILTLLFW
                     LLAARIVMPAAWAPFAALGTLLLPVSVWIGVRDVTHTLLVSCSVAAFWWALLRQLQRP
                     TAGGFVALGVAVAAAVLSKYNAVLVIAAALLAAASLPAPRRALRAPGSFWTPLVAAAL
                     LAPHAAWVWSNWGRATAETLEKMQRGSASDSWLVGVLTGLGSLLPLLVATLLPWALAL
                     TWAFGAGVWRRGDRATTADSSLPPPWLRALLARYVLLVLAGLLAMVLLGTTRFDGRWL
                     HPLLACVPVLGFGWLVRAPPGARARRRWWGLLTALAVLMLVLPAVDAWRDARRGTPER
                     FNWPLALMARELRDAGYDGRSLIVADHLTLGGSLRIPFPHAPVVACDAAKKRSVACVA
                     HHVQAARRAGQAWLLVAAEDEAPPAWWSAAAPLDETGPPLQQRALPYRGAGAGAAPMR
                     FVFRWQPPPRDGQAKD"
     gene            complement(6011..7243)
                     /locus_tag="PKV30_01850"
     CDS             complement(6011..7243)
                     /locus_tag="PKV30_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL28996.1"
                     /translation="MSTPAAPPPPSAWWSLTVTTAIQAMVSMALLTLPAMAPAVARAL
                     AVPSTLVGAYVALCYFAAMLSSLVGGTLVRRLGAIRVSQLGLGLCAVGLALCALPWLP
                     AVALGALCIGAGYGPITPASSHLLALTTPAHRMSLVFSIKQTGVPAGGMLAGALVPGL
                     SLWLGWQGTLLAVAGVCALCAALAQPLRAALDADRQPAQPLALQALFKPIGMVLGQPS
                     LRALAACSFLFSAVQVSTTAYMVTYLNESLGMTLLAAGAALSVSQLAGVVGRIAWGAV
                     ADRGLGPRNTLMLLATLMMAGSVLTALLQPQWPTLLIWAIVALLGASAIGWNGVYLAT
                     VARQAPPGQAGVATGGTLLFTFMGVVCGSPAFGALAGASGSYRIAFAALALPAALALG
                     LLVWRAAQARREPAAPKP"
     gene            complement(7412..7831)
                     /locus_tag="PKV30_01855"
     CDS             complement(7412..7831)
                     /locus_tag="PKV30_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HRL28997.1"
                     /translation="MTTHPAAEPTPAAPATPFDWIGGEPAVHALVERFYDLMDLEPGY
                     AAVRATHGSTLADARQKLFWFLCGWLGGPDHYIERFGHPRLRMRHLPFSIGIRERDEW
                     LACMDQAMRETGVDEALRQRLSQSFFQTADWMRNKGG"
     gene            complement(8328..9314)
                     /locus_tag="PKV30_01860"
                     /pseudo
     CDS             complement(8328..9314)
                     /locus_tag="PKV30_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            complement(9518..11566)
                     /locus_tag="PKV30_01865"
     CDS             complement(9518..11566)
                     /locus_tag="PKV30_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HRL28998.1"
                     /translation="MRTVSPPPSRRFMLCSLTAAALVLTACGGSDDNNTPADLAGSSA
                     TLAVLSTTDLHTNILGYDYFKLAADPSYGFDRAATLIRSARSEFANSLLVDNGDTIQG
                     TALADYQAQVSPLPCTQQLAMYKAMAVLKYDAGTLGNHEFNYGLPFLNQVLGGGLDVE
                     GVDKNQKCAGAGFPMALANVYSVKSGQPLVAPYQIIERQLSATDKDGKPVTLPVKIGV
                     IGFTTPGILNWDKRFVEGKLRIDGAVESARKYVPELRAKGADVVVALLHGGLDASAYQ
                     PTMENPGLHLAQVPGIDAMVMGHQHGVFPDRGANPGYKQPGVDNAAGTVNGVPAVMPS
                     SWGKALGVIALKLKWDGKAWSIDKAATQVEARSTVTGKDAAGKTTYVAADSAVAAAVQ
                     AEHQAAIEYVKTPIGQTDFRMSTLLADVGDPGAIQLVNQAQQDYVSRYIQASLPQYAS
                     LPVLSVSAPFKSGFQGPADYTDVAAGPLAINSAADLYLYPNTVYAVKVNGAQIKDWLE
                     AAAQRFNQIDPAKTAPQQLISSFPGYNFDMFTTPEVQYEIDVTQAKGNRIKGLTYQGQ
                     PMSATQDFVIATNNYRATSGRGFLPVLDGTATIYASPDANRDVVISYIKAHPQVTRAA
                     NGSAKSWRFARTPTAGKVLFSGGPNALGVAQAAGLTNIALEQADDGAGRSVYSIDLGR
                     "
     gene            complement(11740..14265)
                     /locus_tag="PKV30_01870"
     CDS             complement(11740..14265)
                     /locus_tag="PKV30_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRL28999.1"
                     /translation="MNTSFWALGWRTLWRDLRSGELRLLMVAVTLAVAALTAVGFFSD
                     RLQAGLQRDARQLLGGDAVVVSDNPPAAPWVEQADRLKLRHVLTLTFPTMGRADDAQG
                     GATRLVALKAVGDGYPLRGQLRLARDAADREGQPTSGIPERGTVWVDAALLEALALKS
                     GDALLLGDARFRIAELIALEPDRGTGFMTFAPRVMLNAADLSATGLVQPASRITYRLA
                     VAGDEPAVRQYLTWAEAEVKKPEVHGVRLESLGSGRPEMTRTLDRAEKFLSLVALLAA
                     LLSAVAVALAARGFAARHLDDCAMLRVLGLPQRAIAGAYTVEFVLVGLAASAAGVLIG
                     YAVHHVFVALLAGLVDAALPPPSVWPVLFGIGIGLTLLVAFGLPPVLQLAQVPPLRVL
                     RRDVGALKPASLAVLGLGVAGFAALLLAVSSDLTLGLIAVGGFAGAAAVFALLSWVAV
                     KGLRRAVNEQTAPRWLVLATRQMSARPAYAVVQISSLAVGLLALVLLVLLRTDLISSW
                     RAATPADAPNRFVINVMPDQGEAFRATLKDAGVTKMDWYPMFRARLVAINGKPVNPTD
                     FTDDRAQRLVDREFNLSHAAERPPHNEISAGRWQPNEAGAVSVEQGLAETLGLKLGDR
                     MTFDIAGTPAEGKITSLRKVDWASMHANFFVMFPLAEMPDMPITYIAAYRAPGTSGFD
                     NALVRQFPNITNVDMSATLAQVQRVLDQVIRAVEFLFGFTLVAGLIVLFAAVSATREE
                     RAREFAIMRAVGASSRLLGDVQRAELVGVGLLAGFLASAVAVGVGWAMARYAFEFNWT
                     ASPWVPLAGSAVGAVLALVAGWWGLRDVLRRPVVETLRRAAAE"
     gene            complement(14363..15223)
                     /locus_tag="PKV30_01875"
     CDS             complement(14363..15223)
                     /locus_tag="PKV30_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRL29000.1"
                     /translation="MNARIPADVPRLTEAKAPLTDEIEVTDEHGQRQRASIPAERALT
                     VYVDKREIVTLMTLGAHPEWLVLGYLRNQRLVASADEIEAITVDWEVGAAAVTTRRGI
                     ERIAERTAQRVVTTGCGQGSVFGDLMADIDAIDLGAGHITQAQLYGVVNAIRLQESTY
                     KSAGSVHGCALFQGERMLIFVEDVGRHNAIDTIAGWMWLQDVATQRGDDKLFYTTGRL
                     TSEMVIKSAQMGVPIVVSRSGITQMGWQVARRLGLCAIGRAMNKRFLCYSAPERLQWQ
                     PELAGGSILN"
     gene            complement(15424..15774)
                     /locus_tag="PKV30_01880"
                     /pseudo
     CDS             complement(15424..15774)
                     /locus_tag="PKV30_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15786..17009)
                     /locus_tag="PKV30_01885"
     CDS             complement(15786..17009)
                     /locus_tag="PKV30_01885"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRL29001.1"
                     /translation="MTLFNAAVVSLAAIGASWAQSAPASAADAASAPAAATAPAPAPA
                     SAPAAAMPGIRSANIFEIAPGAASDPNYANQTNGERALVQPGNNAPMWRAVGAGATGY
                     SSLPKSEAPEAGNLIQPKVQYPGSRFTSAGEAWRQVRNHWLLPYGAALLGIVVLALAI
                     FFFTIGPLGHREPEGTPRPIERFTPFERAAHWTNAAAFILLALSGLTMAFGKYFLLPV
                     LGLHLSGWLTYLLKNVHNFVGPFFAVSLLVIILTFVKDNIASLADINWLAKLGGMFSK
                     EQVPSHRFNAGEKGIFWWAVTFPGIVVVASGFVLNQLVPNMQFLRGDMQIAHMIHLTA
                     GLIMMALILGHIYMGTVGMHGAYKAMRTGYVSEEWAREHHQLWYDDIVAGKIPAQRTT
                     EPAPAQPPIAPAAPI"
     gene            complement(17031..17288)
                     /locus_tag="PKV30_01890"
     CDS             complement(17031..17288)
                     /locus_tag="PKV30_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29002.1"
                     /translation="MKARTLLCAAACAAVLALAGCGEKSQDLGTSAKLDAPPYDGTGV
                     ASFTAPGWKAGDANSWTQELRTRGQYGQNEYTRIVNTTANK"
     gene            complement(17285..>17583)
                     /locus_tag="PKV30_01895"
     CDS             complement(17285..>17583)
                     /locus_tag="PKV30_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HRL29003.1"
                     /translation="CAGGPEKNGSQAEFEKYGRNRLAEGKLPACAEMCSTKALLAGDG
                     DMVADIFRTRVVARGKGAEVWGWGTAYGNSGTATPPAGGAGTSAPNAQPSGGKS"
BASE COUNT         2798 a         6084 c         5985 g         2716 t
ORIGIN      
        1 tggcggagaa ggagggattc gaaccctcga tacggagaac cgtataccgg atttcgagtc
       61 cggcgcattc gaccactctg ccacctctcc tgtgttcgtg tcgaactgcg aattctatca
      121 tcgcagtaca gcgcttacgg acgcaacacc tccaggccgc ccaggtacgg tcgcaacgcg
      181 gcaggaatcg cgatgctgcc gtcggcttgt tgatggtttt ccagcacggc gaccaacgcc
      241 cgccccacgg ccaggccaga gccgttcaat gtatgcacca gctcgttctt gcccgccgcg
      301 ttcttgaagc gcgcctgcat gcgccgggcc tggaaagcct cacagttgct gacggagctg
      361 atctcccggt aagtgtcctg cgccggcacc cagacttcga ggtcgtaggt tttgctggcg
      421 ccaaaaccca tgtcgccggt gcacagcagc acgacgcggt agggcagctc cagtttctgc
      481 agcacggctt cggcgtggcc gaccatcgcc tccagcgctt cgtagctctt ctcagggtgg
      541 gtaatctgca ccatctcgac tttgtcgaac tggtgctggc gaatcagccc gcgcgtgtcg
      601 cggccggcac tgcctgcttc cgagcggaaa cacggcgtgt gcgcggtcag catgatgggc
      661 agctcgctct cgtccacgat ctcgccgcgc accagattcg tgagcgacac ctcggaggtg
      721 gggatcaggt agagcgattc gccctcgcct tcctgccccc cttttttggc cgcgaacaaa
      781 tcggcttcga acttgggcaa ctggccggtc ccgcgcagcg tgtcggcgtt gacgatgtag
      841 ggggtgtagc actcggtgta gccgtgctgc gtggtttgca ggtcgagcat gaagtgcgcg
      901 agagcccgat gcagccgcgc gatcgggccg cgcatgaagt tgaagcgcgt gccggccagc
      961 ttgacgccgg tttcgaaatc cagccccagc ggctcgccca gttcgacgtg gtcgcgcacc
     1021 ggaaagtcaa acgcgcgcgg agccaggccg tgcggcgccc agcggcgcac ctcgacgttg
     1081 gcgtgttcgt cggcgcccac cggcacgctt tcgtgcggca ggttgggcac ggccagcagc
     1141 agcgtggcca tctcgtgctg gatgacgtcg agccgcgcgg cggacgcatc cagttcgtcc
     1201 ttgatcgacg ccacttgcgc catcaaatcg tcggcggatt cacccttggc cttgcgctgg
     1261 ccgatctgct tgctggcgct gttgcgctgc gcctgcaatt cttcggtgcg ggtctggatg
     1321 gtcttgcgct cgctctccaa cgcggagaaa gacgcttcgt cgagaaaggg ctgcgggctt
     1381 ttgcgggtgg ccaggcgcgc cagcacggcg cccaaatctt tgcggagcag gatgggatca
     1441 agcatgcgcc gattgtaggc ggcgcgcacg gcgccaatcg atggcgcgcc gtgccaaagg
     1501 atttaaatga aaaccggcgc cagcgcttgt caatcaagcg caagcagcta tcaaaaacaa
     1561 agtaatccgg tcagctcatg tggcccagtt gcgactcgcc ctgctggtcg agtttgaggc
     1621 ccttcggcag cgggaacttg accgtctcct cgatgccatc gagcttgcgc accgcggtgg
     1681 cgcccagggc gcgcaggcgc tcgatgacct gccgcaccaa gatgtcgggc gccgacgcgc
     1741 cggccgtgag gccaatgcgc gacttgccct cgatccattc ggcgcgcaac tcatcagcgc
     1801 cgtcgaccat gtaggcatcg acacccagct tgcgcgccac ttcgcgcagt cggttgctgt
     1861 tgctgctggt cgggctgccg acaacgatga ccacatcgac ctcgctcgac agcagcttga
     1921 ccgcgtcttg ccggttctgc gtggcgtagc aaatgtcctg ctgtttcggc tcgcgcactt
     1981 gcggaaagcg cgccttgacg gcggcgctga tctcggccgc gtcgtccacg ctcagcgtgg
     2041 tctgcgtcac cacggccagc ttgtcggttt gcgcgggctg cacgcgcgcc acgtcggcca
     2101 cgtcttcgac caggtgaatg ccgctgtcaa gctggcccat ggtgccttcg acctcggggt
     2161 ggcccttgtg gccgatcatg atgaattcgt aaccttcctt gtgcagcttg gccacttcaa
     2221 cgtgcacctt gctgaccagc gggcaggtgg cgtcgaacac gcgaaagccc cggctttctg
     2281 cttcacgctc gacggacttg ggcacgccgt gcgcgctgaa caccagtgtg gcgcccaccg
     2341 gcacgtcttc gagtgcttct ataaaaatag cgcccttggc tttcaggtcg ttcaccacgt
     2401 aggtgttgtg cacgatctcg tgccgcacgt agatcggcgc gccaaacttg gcgatggcgc
     2461 gctcgacgat ctcgatggcg cggtcaacgc cggcgcaaaa gccgcgcggc tcggccagca
     2521 agatttcagc cttgttcatc acagcacccc gatcaattcg acctcaaacg tcaccggctg
     2581 gccagccagc gggtggttga agtcgagttg caaggcgccg tcatcacgca ccgcctgcac
     2641 cacggcggca aactggccct ggccgtcggg cgtgggcagc tggatcacat cgccgatggc
     2701 gtagcgctcg ttggggtcgc ccaggctgtc gagctccttg cgcgccagcc actgaaccat
     2761 gtccgggttg cgctcgccaa aggccgcgcc ggcgggcaac tcgaaggtgt cgtgcgcgcc
     2821 ttcagccagg ccgatcaagc gatcttcgac ggcgggcgac agctcgcccg cgcccagcga
     2881 cagcgtggcg ggcttgccgc caaaggtgtt gatgacatcg ccctgcggcc cgctcaggcg
     2941 gtagtgcagg gtcaaaaaag aacccagttc gacggtggtc atgcatgccc cagataaact
     3001 tccctcgatt gtagaaagcc cctgcgccgc cgcacccgat ggccctgaaa gaccttccgc
     3061 ccgacgcccg cccgcgcgag aaactgctcg cgcgcggcgc cggcgccttg agcgatgccg
     3121 agttgctggc gctgctgctg cgcaccggca cggccgggcg cggcgtgctg caaatggcgc
     3181 aagagctgct ggcacagttc gagggcgtgg ctggcttgct gcacgccagc gccgatgacc
     3241 tgaagcgctt caagggtttg ggcggcagcg ccaagcgggc cgagctggtg gccgtgcttg
     3301 aactcgcgcg ccgcgcgctg gccgagcagc tgaaggagcg cacggccttg aattccgcgc
     3361 aggcggtcaa gcactacgtg cagctgcacc tgggcgcgcg cccgcacgag gtgttcgcgg
     3421 cgctgtttct cgacagccag catcgcctga tcgcgctgga agagctgttc cgcggcacgc
     3481 tgtcgcagac cagcgtctac ccgcgcgagg tggtgatacg cgccctgcac caccatgcca
     3541 gcggcgtgat cctggcgcac aaccacccga gcggcggcgt gcagcccagc cgcgccgacg
     3601 aatcgctgac gcagaccttg aaagccgctt tggcgctggt cgatgtgcgt gtggtggatc
     3661 acgtcatcgt ggcgccgggc gcggcgctgt cgatggctga gaaggggttg gtgtgaaggt
     3721 caaatcgctg caggacttga aggcgctgca aaagcagttg gctgagaccc aggcccgcgc
     3781 cgccaaggaa gccgcagtgc gcgcggccgc cgagcaaaag gcgcgcgccg aacagaacct
     3841 gtttgcgcgc gccatcggcg ccacacagcc gctgcgcgcg cacggccggg cggtgcacgc
     3901 gccggccccg gtgccgcctg agccgctgca gcgccagcgc gacgagcagg ccgtgctgcg
     3961 cgaagccatc tcggacgaat tcgacgccag cacgctgctg caggtggatg aacacctgag
     4021 ctttcgccgc cccggcatcg gccccgacgt attgccgaag ctgcggcgcg gccattgggc
     4081 ggtgcagcgg cagatcgacc tgcacggcct gcgcaccgag gaagcgcgcg aggcgcttgg
     4141 tcagttcgtc cgcgacgcgc agcgcgccgg cgtgcgctgc gtgcgcgtgg tgcacggcaa
     4201 gggcctgggc tcgcccggca agacgccggt gctcaaggcc aaggtgcacg gctggctgat
     4261 ccagaagaag gaagtgctgg ccttcgtgca ggccaagccg ctcgaaggcg gcgccggcgc
     4321 gctgctggtg ctgctcgcat cggcgcagtc gatgggctga gcgcctgttg gtatcagtct
     4381 ttcgcctgcc cgtcacgtgg cggcggctgc cagcgaaaca cgaagcgcat cggcgctgcg
     4441 ccggcacccg cgccgcggta cggcagcgca cgttgttgca gcggcggccc cgtttcatcg
     4501 agcggcgcgg ccgccgacca ccacgccggt ggcgcctcgt cttcggcagc caccagcagc
     4561 caagcctggc cggcgcgccg ggccgcctgc acatggtgcg ccacgcaagc caccgagcgc
     4621 ttcttggccg cgtcgcaagc caccaccggc gcgtgcggaa acggaatgcg caagctgccg
     4681 cccaaggtca ggtggtcggc cacgatcagg ctgcggccgt catagccggc gtcgcgcaac
     4741 tcgcgcgcca tcaaggccaa cggccagttg aagcgttcgg gcgtaccgcg ccgcgcgtcg
     4801 cgccaggcgt caacggccgg cagcaccagc atcagcacag ccagagcggt gagcaagccc
     4861 caccagcggc ggcgcgcgcg ggcgcctggc ggcgcgcgca ccagccaccc gaagcccagc
     4921 accggcacgc acgccagcag cggatgcagc caacggccgt cgaagcgcgt ggtgcccagc
     4981 agcaccatcg ccagcaggcc ggccagcacc agcagcacgt agcgcgccag caaggcgcgc
     5041 agccacggcg gcggcagcga cgaatcggcg gtcgtagcgc gatcgccgcg tcgccagacg
     5101 ccggcgccga aggcccaggt caaggccagc gcccacggca gcagggtcgc caccagcagc
     5161 ggcagcaggc tgccgagccc ggtcaaaacg ccgaccagcc agctgtccga cgccgagccg
     5221 cgctgcattt tttccagcgt ctcggcggtc gcccgccccc agttcgacca cacccacgcc
     5281 gcgtgcggcg ccagcagagc ggcggccacc aacggcgtcc agaacgaccc cggcgcccgc
     5341 aaagcgcgtc gtggcgccgg cagtgaagcg gccgccagca gcgcggcagc aatcaccagc
     5401 acggcgttgt atttggaaag cacggccgcc gccaccgcaa cccccagggc gacgaagccg
     5461 cccgccgtcg gccgctgcaa ctgacgcagc agcgcccacc agaaggccgc cacgctgcag
     5521 ctcaccagca gcgtgtgtgt cacatcgcgc acgccgatcc acacgctgac cggcagcagc
     5581 agcgtgccga gcgccgcgaa cggcgcccat gcggccggca tcacgatgcg cgcagccagc
     5641 agccagaaca gcagcgtgag gatcatcagc acgcccttca acagggccag tgccagcacc
     5701 gacggcccca gcagctcgtt ggcgccccac tgcagccagg tgtagagcgg tggctgcggc
     5761 ccataacccc aggccagctg ctgcgaccac agcatctgct cgccgtagtc ccatttcagc
     5821 gcctctgaaa gcgacagccg aagcatcacc cacgccacga cccacgccag cacggcccac
     5881 gccgtcgctc gcgccggcac agcgcgttct gctggcagat cggtcgattg aggtcgagat
     5941 gcgggcatgg cgatcaggct tcatcctagc gctggcagcg cgcgacaaag ccgcgccgct
     6001 cggccctgca tcaaggcttc ggcgccgcgg gctcgcgccg cgcctgtgcc gcgcgccaca
     6061 ccagcaagcc cagcgccagc gcggccggca gcgccagcgc ggcgaaggcg atgcgatagc
     6121 tgccgctggc cccggccagc gcgccaaacg ccggcgagcc gcacaccacg cccatgaagg
     6181 tgaacagcag cgtgccgccc gtcgccacgc cggcctgccc cggcggtgcc tggcgcgcca
     6241 cggtagccag ataaacgccg ttccagccga tggccgaggc gcccagcagc gccacgatgg
     6301 cccagatcag cagcgtcggc cactgcggct gcagcaaggc cgtcagcacg ctgcccgcca
     6361 tcatcagcgt ggccagcaac atcagcgtgt tgcgcggccc gaggccgcgg tcggccaccg
     6421 cgccccacgc aatgcgcccc acgacacccg ccaattgcga caccgacagc gccgcgccgg
     6481 cggccagcag cgtcatgccg agcgattcgt tgaggtaggt gaccatgtac gccgtggtcg
     6541 acacctgcac cgccgaaaac agaaacgaac acgccgccag cgcgcgcagc gacggctgcc
     6601 cgagcaccat gccgatcggt ttgaacagcg cctgcagcgc cagcggctgg gccggctggc
     6661 ggtcggcgtc gagcgcggcg cgcagcggtt gcgccagcgc cgcgcacagc gcgcacacgc
     6721 ccgccacggc cagcagcgtg ccctgccagc ccagccacag cgacagcccc ggcaccagcg
     6781 ccccggccag catgccgccg gccggcacgc cggtctgctt gatcgagaac accagcgaca
     6841 tgcggtgcgc cggcgtggtc agcgccaaca ggtgcgagct ggccggcgtg atcggcccat
     6901 agccggcgcc gatgcacagc gcgcccaacg cgaccgcggg cagccacggc agcgcgcaca
     6961 gcgccagccc gacagcgcac agccccagcc cgagctggct gacgcggatg gcgcccagcc
     7021 gccgcaccag cgtgccaccg accaggctcg acagcatggc agcgaagtag cacagggcca
     7081 cgtaagcgcc caccagcgtg gaaggcaccg ccagcgcgcg cgccaccgcc ggcgccatgg
     7141 ccggcagcgt gagcagcgcc atcgacacca tggcctggat ggcggtggtg acggtgaggc
     7201 tccaccaggc gctgggtggg ggtggtgccg ccggggtact catgtcgacg gcgcgtggtg
     7261 gcatggccac gcgggccgtg cacggtgacc aatgaccgcg ctgtggcgag cgcatgaaaa
     7321 atgaccaaag gcatcacctg acgcggcgcc ttggtcatgg gtcatggcgc gccagcgccc
     7381 atcattgagc gcagcaaagt catgcttggt gtcagccacc cttgttccgc atccagtcgg
     7441 cagtctggaa gaagctctgg ctcaggcgct gccgaagtgc ctcgtccacg cccgtctcgc
     7501 gcatcgcctg atccatgcag gccagccact cgtcgcgctc gcggatgccg atggaaaacg
     7561 gcaggtgccg catgcgcagg cgcgggtggc cgaagcgctc gatgtagtgg tccggcccgc
     7621 ccagccagcc gcacagaaac cagaacagct tttgccgcgc atcggccagc gtgctgccat
     7681 gcgtggcgcg cacggcggcg tagccgggtt ccaggtccat caggtcgtaa aagcgctcga
     7741 ccagcgcatg caccgccggc tcgccgccga tccagtcgaa gggcgtggcg ggcgctgccg
     7801 gtgtcggttc agcggctgga tgggtggtca aaataggctc tggcgcttgt ctgacaagcg
     7861 cgagcagcta ttttttatgt agcgacgcgc gctggaagga tacgcgcgga ttctaggtcg
     7921 tcgcgcgccg tgcgccgtgc gtgccgctgg cgccatcgtg gtgcgatgcg ctcatcgcca
     7981 atttcaagct gaaacaggcc gcagcgctgg tcagtcaagc gccagcagct gctttttcag
     8041 tagcgcccgg cgcctgccga ccgccctcac gccgccgccg cgccgctggc gcgcacggcc
     8101 tgccgcggcg ccttgtcgtc gcccggcgcc acctcggcgc tggccgcgcg cacggcgcgg
     8161 gccgcggcct ggttggccac gtcggcggca atgtgctgct cttcgggcga ccagcgaaag
     8221 cgcttgcgct cgatctcatc ggccaggtgg tcgtcgccat cggcgtcgtt gccggcgtcg
     8281 gcggccactt cgtactgcag cttgagcgga tcgccgtagt cgcgcttcag gttgaccgaa
     8341 cttttctcgc ccaccgcgtc gtagtggcgc ttgagccagc gctgagccga ggcgcggccc
     8401 agttcaaaca gcttttccag cgtggcgcgc tgcggcgata tcttgctggc cgccggcatg
     8461 tcggccagcg cgctgccgcc gtcgatgcgg tgcatgtgga tgggtttcag gccctcgccc
     8521 gtcatgccgt gcgccaccag ctcgttcagc agcgcgatgc tgcgcatctg cgcgatcagg
     8581 ctggcgttga aggtcaaatc gttcgcgcgg tcggtgattt cggccgccgt ggtcgcacgg
     8641 ccggcgcgct tgagcgggtt gatctgcacc agcagcacgt cgctggtttc gcccatctcc
     8701 agcagcgggc cgatggcggg gttggccgag tagccgccgt cccaataggt cttgccgtcg
     8761 atctcgggcg cctgaaacag ctgcggcagg caggccgagg ccatcacggc cgccgccgtg
     8821 agccgcttgc cactgaagat ctcggcgcgg ccggtttcga cgtcggtggc ggcgatgaac
     8881 acgcgcggcg acttcagctt ttgcagccgc tcgaaatcga tttcgcgctc gatcaactgg
     8941 cgcagcgggt tgatgtcgag cgggttggtc tggtagggcg aaaaacgcgt cagcgcaccc
     9001 ggcagcgcgc ccatcatcag cttggtcagg cgcgccgtgc tttcgctggc ggcgcccagt
     9061 tccatgacct ggttccacac gcgggccagg ctggcgcgcg cgccttcgta cgggtcctgc
     9121 ttgtccgcca cggcctgcgc ccagccgtgc gccagcgcca cggcattgac ggcgccggcg
     9181 ctggtgccgc tgatgccggc gaagtcgagg cgcccgtcct gcagcagcgc gtccagcacg
     9241 ccccaggtga aggcgccgtg cgcgccgccg ccctgcaggg ccaggtggat ggttttggtg
     9301 gtggtatcac tcatgccttc agtgaaccac aagcagacga aaagttttag gtagaaaccc
     9361 gcaatatcgg tgttgaccta cagagagcaa gctctaattt cgttgtcaac gccgcgcttg
     9421 taagaccagt ttttcaaggc aaaaaacggc gccagcgctt gatggataag cgctggcagc
     9481 tatcacaaca atagtatcga tgcaccgtgg cggtgcgtca gcgccccagg tcgatcgaat
     9541 acaccgagcg gcccgcaccg tcgtcggctt gctccagcgc gatgttcgtc aggcccgccg
     9601 cctgcgccac gcccagcgcg ttcgggccgc cgctgaacag caccttgccg gccgtcggcg
     9661 tcctggcaaa gcgccagctt ttggcgctgc cgttggcggc gcgggtgact tgcgggtgcg
     9721 ccttgatgta gctgatcacc acgtcgcggt tggcgtccgg gctggcgtaa atggtggccg
     9781 tgccatccag caccggcaaa aagccgcgcc cgctggtggc gcggtagttg ttggtggcga
     9841 tgacgaagtc ctgcgtggca ctcatcggct ggccctggta ggtcaggccc ttgatgcggt
     9901 tgcccttggc ctgcgtgacg tcgatctcgt actgcacctc gggcgtggtg aacatgtcga
     9961 agttgtagcc ggggaaactg ctgatgagct gctgcggcgc ggtcttggcc gggtcgatct
    10021 ggttgaagcg ctgggccgcg gcctccagcc agtccttgat ctgcgcgccg ttcactttca
    10081 ccgcgtacac ggtgttgggg tacaggtaca agtcggccgc gctgttgatg gccagcgggc
    10141 cggccgctac gtcggtgtag tcggccgggc cctggaagcc gctcttgaac ggcgcgctga
    10201 cgctcagcac cggcaggctg gcgtactgcg gcaggctggc ctggatgtag cggctcacgt
    10261 agtcctgctg cgcctggttg acgagctgga tcgcgcccgg atcgcccacg tcggccaaca
    10321 gtgtgctcat gcggaaatcg gtctggccga tgggcgtctt gacgtactcg atggccgcct
    10381 ggtgctcggc ctgcacggcc gcggccacgg cgctgtcggc ggcgacgtag gtggtcttgc
    10441 cggccgcgtc cttgccggtg acggtgctgc gcgcctcgac ctgggtcgcg gccttgtcga
    10501 tggaccaggc cttgccgtcc cacttcagtt tcagcgcaat cacgcccagc gccttgcccc
    10561 atgagctggg catcaccgcc ggcacgccat tcacggtgcc ggcggcgttg tccacgcccg
    10621 gctgcttgta gccggggttg gcgccgcggt cggggaacac gccgtgctgg tggcccatca
    10681 ccatggcgtc gatgccgggc acctgggcga ggtgcaggcc ggggttttcc atcgtcggct
    10741 gataggccga agcgtccagc ccgccgtgca gcagggcgac caccacgtcg gcgcccttgg
    10801 cgcgcaactc gggcacgtac ttgcgcgccg attccaccgc gccgtcgatg cgcagcttgc
    10861 cttcgacaaa gcgcttgtcc cagttcagga tgccgggcgt ggtgaagccg atcacgccga
    10921 tcttgacggg cagcgtaacg ggtttgccgt ccttgtcggt ggccgacaac tggcgctcga
    10981 tgatctgata cggcgccacc agcggctggc cgcttttcac gctgtaaacg ttggccagcg
    11041 ccatcggaaa gccggcgccg gcgcacttct ggttcttgtc gacgccctcg acatccagcc
    11101 cgccgcccag cacctggttc aaaaacggga gaccgtagtt gaactcgtgg ttgcccagcg
    11161 tgccggcgtc gtacttcagc accgccatcg ccttgtacat ggccagttgc tgcgtgcacg
    11221 gcagcgggct gacctgcgcc tgatagtcgg ccagcgccgt gccctggatg gtgtcgccgt
    11281 tgtccaccag caggctgttg gcgaactcgc tgcgcgcgct gcgaatcagc gtggcggcgc
    11341 ggtcaaagcc gtagctcggg tcggcggcga gcttgaaata gtcgtagccg aggatgttgg
    11401 tgtgcaggtc ggtggtcgac agcacggcca gcgtggcgct gctgccggcc aggtcggcgg
    11461 gcgtgttgtt gtcgtcgctg ccgccgcagg cggtgaggac gagcgcggcg gcggtcaggc
    11521 tgcacagcat gaagcggcgg gaaggcggtg gtgaaacggt gcgcatggct cggaacggct
    11581 tgaagaaaag ccgcatgctg cgctgcccgt gtgacagccg gttgaccgca cggtgtcacg
    11641 gcgaagaccg tcattcatga tcaaaacagg ctgcagcgct tgtccatcaa gcgctggcag
    11701 ctattcaaat catagcgatt tgaagcatcg cgtgacaccc tactcggccg ccgcgcggcg
    11761 cagcgtctcg accaccggcc gccgcagcac atcgcgcagg ccccaccagc cggccaccag
    11821 cgccagcacg gcgccgaccg cgctgccggc gagcggcacc cagggcgagg cggtccagtt
    11881 gaactcgaac gcataacgcg ccatggccca tccgacgccc acggcgactg cgctggccag
    11941 aaagcccgcc agcaaaccga cgcccaccag ctcggcacgc tgcacgtcgc ccagcaggcg
    12001 gctggaggca ccgacggcgc gcatgatggc gaactcgcgc gcgcgctctt cgcgcgtggc
    12061 gctcaccgcc gcaaacagca cgatcaggcc cgccaccagc gtgaagccga acaggaactc
    12121 caccgcgcga atcacctgat ccagcacgcg ctgcacctgc gctagggtgg cgctcatgtc
    12181 cacattagtg atgttgggaa actggcgtac cagcgcgttg tcaaagcccg aagtgcccgg
    12241 cgcacggtag gcggcgatgt aggtgatcgg catgtccggc atctcggcca gcggaaacat
    12301 gacgaagaag ttggcgtgca tcgacgccca atcgaccttg cgcagcgagg tgatctttcc
    12361 ttcagccggc gtgccggcga tgtcgaaggt catgcggtcg cccaatttaa gccccagcgt
    12421 ctcggccagc ccctgctcga cgctgacggc gcccgcttcg ttcggctgcc agcggccggc
    12481 gctgatctcg ttgtggggcg ggcgctcggc cgcgtgcgag aggttgaatt cccgatccac
    12541 cagccgctgc gcgcggtcgt cggtgaagtc ggtggggttg accggcttgc cgttgatggc
    12601 caccagccgg gcgcgaaaca tggggtacca gtccatcttc gtcacgcctg catccttcaa
    12661 ggtggcgcga aatgcctcgc cctggtccgg catgacgttg atgacaaagc ggttcggcgc
    12721 atcggccggc gtggcggcgc gccagctgct gatgaggtcg gtgcgcagca gcaccagcag
    12781 caccagcgcc agcaggccga cggccaggct gctgatctgc accacggcgt aggccggccg
    12841 cgccgacatc tgccgcgtgg ccagcaccag ccagcgcggc gcggtctgtt cgttgacggc
    12901 gcggcgcaag cccttgaccg ccacccacga cagcagcgcg aacacggccg ccgcgccggc
    12961 aaagccgccg acggcgatca ggcccagcgt caagtccgag ctgacggcca gcagcagcgc
    13021 cgcaaagccc gccacgccca gacccagcac cgccagcgac gcgggcttga gcgcccccac
    13081 gtcgcgccgc agcacgcgca acggcggcac ctgcgccagt tgcagcaccg gcggcaggcc
    13141 aaaggcgacc agcagcgtca gcccgatgcc gatgccaaac agcaccggcc acacgctggg
    13201 cggcggcagc gcggcatcga ccagcccggc cagcagcgcc acgaacacgt ggtgcaccgc
    13261 gtagccgatc agcacgccgg ccgcgctggc ggccagcccg accagcacga actcgaccgt
    13321 gtacgcaccg gcgatggcgc gctgcggcag gcccagcacg cgcagcatgg cgcaatcgtc
    13381 cagatgccgc gccgcaaagc cgcgcgcggc cagcgccacc gccacggccg acagcagcgc
    13441 cgccagcagc gccaccaggc tgaggaactt ctcggcgcgg tcgagcgtac gtgtcatctc
    13501 gggtcggccg ctgcccagcg attccagccg cacgccgtgc acctccggct ttttcacctc
    13561 ggcctcggcc caggtcaggt attggcgcac cgccggctcg tcgcccgcca cggccaggcg
    13621 gtaggtgatg cgactggccg gctgcaccag gccggtggcg ctcaggtcgg cggcattcag
    13681 catcacgcgc ggggcgaagg tcatgaagcc ggtgccgcgg tcgggttcca acgcgatcag
    13741 ctcggcaatg cgaaaacggg cgtcgcccag cagcagcgcg tcgccgcttt tcagcgccag
    13801 cgcttcgagc agcgcggcat cgacccaaac cgtgccgcgc tcggggatgc cggacgtggg
    13861 ctggccttcg cggtctgcgg catcacgcgc cagccgcagc tgaccgcgca gcggatagcc
    13921 gtcgcccacc gccttcaacg ccaccagccg cgtggcgccg ccctgtgcgt cgtccgcgcg
    13981 gcccatggtc gggaacgtga gcgtcagcac gtgcctgagc ttgagccgat cggcctgctc
    14041 gacccacggc gcggcgggcg ggttgtcgct gaccaccacc gcatcgccac ccagcagctg
    14101 gcgcgcgtcg cgctgcaggc cggcctgcag gcggtcggaa aaaaagccca ccgccgtgag
    14161 cgccgccacc gccagcgtga cggccaccat caacagccgc agctcgcccg agcgcaggtc
    14221 gcgccacaag gtgcgccagc ccagcgccca gaaggatgtg ttcatgatgc gcaccttaac
    14281 gcaaggctca gccaagcgtc gcagacgggg atttttgaat gaaaactgcc tccagcgcct
    14341 gatagaacag cgcgagcagc tatcaattta atattgatcc gccggccagt tcaggctgcc
    14401 actgcaagcg ctcaggcgcg ctgtagcaga ggaagcgttt gttcatcgcc cgcccgatgg
    14461 cgcacaggcc gagccgccgc gccacctgcc agcccatctg cgtgatgccg ctgcgcgaga
    14521 cgacgatggg cacgcccatc tgcgccgact tgatgaccat ctcgctggtc agccggccgg
    14581 tggtgtagaa caacttgtcg tcgccgcgct gggtggccac gtcctgcagc cacatccagc
    14641 cggcaatggt gtcgatggcg ttgtggcggc ccacgtcctc gacaaagatc agcatgcgct
    14701 cgccctgaaa cagcgcgcag ccgtgcaccg agccggccga tttgtacgtg ctttcctgca
    14761 agcggatggc gttgaccacg ccgtagagct gggcctgcgt gatgtgaccg gcgcccaggt
    14821 cgatggcgtc gatgtcggcc atcaagtcgc cgaacacgct gccctggccg cagccggtgg
    14881 tgaccacgcg ctgcgccgtg cgctcggcaa tgcgctcgat gccgcgccga gtggtgacgg
    14941 cggcagcgcc gacttcccaa tcgacggtga tggcttcgat ctcatcggcc gacgccacca
    15001 gccgctggtt gcgcaggtag cccagcacca gccactcggg gtgcgcgccc agcgtcatca
    15061 gagtgacgat ctcgcgcttg tcgacgtaga cggtgagcgc gcgctcggcc ggaatcgacg
    15121 cgcgctggcg ctggccgtgc tcgtcggtca cctcgatctc gtcggtgagc ggcgctttcg
    15181 cctcggtcag gcgcggcacg tcagcgggga tgcgggcgtt catggcgaca agcataaagg
    15241 ccacgcgggt gccgcgtggc cttgggtgga tcgacgcagc gcttgcctgg ccgccaccgt
    15301 tcagggcttg gccggcggca ccgtgctggc ggcgggcgcc acgcccgagg ccgcagtggc
    15361 cactggcggc gccgaggcgg ccgtggccgg gctgaacccg ctcttgactt cggcctgcgt
    15421 ctgcggcgtg ttcaccggcg gcgtggcggc ggtggcggcg ggcgaatggg cgccggcctg
    15481 ctccagcggc ttgacctcgg tcggcgtgta gacaaacggg ccggggtcgg tgcacggcgg
    15541 cgtggtcacg gccggggcca gcgtcttgcc ggcggcggcg gcgctttcgc ggtacttggc
    15601 ggccacgcgg tcttgcacct ggcacaggcg gtaaccatcg accttgactt gccaggccgt
    15661 cttggcggcg gcctcgtcgg ccttggcctt ggcttcgggg ctgagcggcg gcagcttggc
    15721 cagcacgccg gcgcagaaac ccagggcgat gaggggtagc aacacgcgtt tcatgcggaa
    15781 acctttcaaa tcggagcggc cggcgcgatg ggcggctggg cgggcgcggg ctcggtcgtg
    15841 cgctgcgccg ggatcttgcc ggccacgatg tcgtcgtacc agagctggtg gtgctcgcgc
    15901 gcccattctt cgctgacgta gccggtgcgc atcgccttgt aagcgccatg catgcccacc
    15961 gtgcccatgt agatgtggcc caggatgagc gccatcatga tcagaccggc ggtgaggtgg
    16021 atcatgtgcg cgatctgcat gtcgccgcgc aagaactgca tgttcggcac cagctggttg
    16081 agcacgaagc ccgaagccac gacgacgatg cccgggaagg tcaccgccca ccagaagatg
    16141 cctttttcgc ccgcgttgaa gcggtgcgag ggcacctgct ctttcgagaa catgccgccc
    16201 agcttggcca gccagttgat gtcggccagg ctcgcgatgt tgtccttgac gaaggtgagg
    16261 atgatcacca gcagcgagac ggcaaagaac ggcccgacga agttgtgcac attcttcagc
    16321 aggtaggtca gccagcccga caggtgcagg cccagcaccg gcagcagaaa gtacttgcca
    16381 aacgccatgg tcaggcccga cagcgccagc agaatgaagg cggcggcgtt ggtccagtgc
    16441 gccgcgcgct cgaacggcgt gaaacgctca atggggcgcg gcgtgccttc gggttcgcgg
    16501 tggccgagcg gcccgatggt gaagaaaaag atcgccagcg ccagcaccac gatgccgagc
    16561 agcgccgcgc cgtagggcag cagccagtgg ttgcgcacct ggcgccaggc ctcgcccgcg
    16621 ctggtaaagc gcgagcccgg gtactgcacc ttgggctgga tcaggttgcc ggcctctggt
    16681 gcctcggatt tgggcaagct gctgtacccg gtggcaccag cgccgacggc gcgccacatg
    16741 ggcgcgttgt tgccgggttg cacgagggcg cgctcgccat tggtctggtt ggcgtagttg
    16801 gggtcggacg cggcgccggg tgcgatctcg aaaatgttgg cgctgcggat gccgggcatg
    16861 gcggccgccg gcgctgaggc cggcgccggg gccggggcgg tcgccgctgc gggcgccgaa
    16921 gcggcatctg ccgcgctggc cggcgcgctc tgcgcccaac tggcgccaat cgccgccagc
    16981 gacaccacgg cggcgttgaa cagggtcatg cagcgcatgt cgacaccccc ttacttattc
    17041 gccgtggtgt tcacgatgcg cgtgtactcg ttctggccgt actggccgcg cgtgcgcaat
    17101 tcctgcgtcc agctgttggc gtcgccggct ttccagcccg gcgcggtgaa cgacgccacg
    17161 ccggtgccgt cgtagggcgg cgcatccagc ttggcgctgg tgccgaggtc ttgcgatttc
    17221 tcgccacagc cagcgagcgc cagcacggca gcgcaggccg cagcgcaaag gagagtgcgc
    17281 gctttcatga tttgcctccg ctcggttgcg cgttcggcgc gctcgtgccg gcgccgcccg
    17341 cgggtggtgt ggccgtgccg ctgttgccgt aggccgtgcc ccagccccac acttcggcgc
    17401 ccttgccgcg ggccaccacg cgggtgcgga agatgtcggc caccatgtcg ccgtcgcccg
    17461 ccagcagcgc cttggtggag cacatctcgg cgcaagcggg cagcttgcct tcggccagcc
    17521 ggttgcgacc gtatttctcg aactcggcct ggctgccgtt cttctcgggg ccgccggcgc
    17581 aga
//
LOCUS       DAOMVO010000017        17307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_643_length_17307_cov_15.937167, whole genome shotgun sequence.
ACCESSION   DAOMVO010000017 DAOMVO010000000
VERSION     DAOMVO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_17307_cov_15.937167"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKV30_01900"
     CDS             <1..366
                     /locus_tag="PKV30_01900"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL29004.1"
                     /translation="AGQLTKMVNQICIAGLVQGLSEAIAFGQRAGLDMNQALGVIGKG
                     AAQSWQLDSRGPTMVEGKFDFGFAVDWMRKDLGLVLDEAKRNGARLPVTALVDQFYAD
                     VQDMGGGRWDTSSLIKRLK"
     gene            complement(416..1192)
                     /locus_tag="PKV30_01905"
     CDS             complement(416..1192)
                     /locus_tag="PKV30_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRL29005.1"
                     /translation="MDFMMTKNRMAARVALALAAGAALAGCVPVALVGGAVGAGTLLM
                     ASERRSNETQEADRTIENAANEAIVQALPGRGHVNLTSFYRKVLVTGEVPTAQDRQLV
                     ESRIRALPGVQGVVNELAVMPESGPLQRSNDSLITSKVRTRLINQNGVPSGSIRVMTE
                     RGTTYLMGRLTAQEATLATEVTRQTDGVQRVVRVIDLIADPTMAGGASPAPAAATAVQ
                     TTGGSAASSPTPGVETHPVTQPTIVNAPQPIQVQPLPPMK"
     gene            complement(1192..1788)
                     /locus_tag="PKV30_01910"
     CDS             complement(1192..1788)
                     /locus_tag="PKV30_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRL29006.1"
                     /translation="MLEQHIQQQFIDSADLKYQWAQSLTRPIVDATQALLACVTGGGK
                     ILICGNGASGALAQYAAALLVGGLERPRPELPALALGTDAATLTAVARRGEYADAFAA
                     QVRALGSAGDVLLVLSSNGNATNLVRAAQAAHEREMSVIALTGRGGGELGHLLRDTDV
                     HVCVPHERTPRIHEAHQLVLHCLCEGLDLQLLGDEESP"
     gene            complement(1953..2339)
                     /locus_tag="PKV30_01915"
     CDS             complement(1953..2339)
                     /locus_tag="PKV30_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRL29007.1"
                     /translation="MALFNLKQTDVTTRLRGNAAEDAALAHLLAAGLTLVERNYRTPG
                     RGGGEIDLVMREAGGTLVFVEVRARAGTTHGGAAASVGGVKQRRIVLAARHYLMRHRS
                     PPPCRFDVVAIDGDHIEWLKAAFDAA"
     gene            2373..3296
                     /gene="rsmI"
                     /locus_tag="PKV30_01920"
     CDS             2373..3296
                     /gene="rsmI"
                     /locus_tag="PKV30_01920"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRL29008.1"
                     /translation="MPNTVAALGFPSYHLALDAARAAAGAQHYPAGALYVVATPIGNL
                     ADVTLRALHVLAIADAIACEDTRRTQQLLRSYGIDGRPLIALHEHNEAEAAQGVIARL
                     QAGERIAYVSDAGTPAVSDPGARLVAACATAGLRVLPLPGASSVTTAISAAGLSGDGR
                     FVFAGFLPNKAGERQTAVDALAQEPRAVVLLEAPHRIAVLGDALARLGERPVTVGREL
                     TKQFEQIATLPCAELPAWLAADAQRQRGEFALVLHPAPGADDSAESERVLRLLLAELP
                     TKSAVKLAADITGAPRNALYERALALKSADQ"
     gene            complement(3367..4614)
                     /locus_tag="PKV30_01925"
     CDS             complement(3367..4614)
                     /locus_tag="PKV30_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRL29009.1"
                     /translation="MILNFVWLGFFLSAFVAAAVHLALGDLTIFPKLLSAMFDAAKTG
                     FDISIGLVGIMALWLGLMKIGERAGMVDAFARGVNPVFRHLFPGVPAGHPAQGAMTMN
                     ISANLLGLDNAATPLGLKAMQELQTLNDRPDTATNAQIMFLVLNTAGLTLIPTSVIAI
                     RQTMAVKQGLVGFNAADIFLPTLVATACSLLSALIAVAAVQRIPLWRAGLLLPLAGFG
                     AVVGALVWWLSRFPPDQAAQWMGVIGAGFILAVVAAFLVAGALRGVAVYDAFVDGAKD
                     GFGVAVQIIPYLVAMLVAIAVFRAAGLMDVLMGGIAAAVSALGLPTDFLPAVPVGLMK
                     ILSGSGARGLMVDVMQTHGVDSFPAKLAAIIQGSTETTFYVLAVYFGSVGVKHTRHAL
                     PCALLADAVGLVAAVAVAYAFFR"
     gene            complement(4798..5601)
                     /locus_tag="PKV30_01930"
     CDS             complement(4798..5601)
                     /locus_tag="PKV30_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HRL29010.1"
                     /translation="MTDSTTPAADNDTDLDDATSATLDTLEDVLDAVRERHPNTPQWE
                     FCEGVLTALLCTRRAIGEDEWLPFIFGRDGDDVFGSAAERTQFSMGWLAREAQLRAAL
                     NAEVDSLEDERALSPAVMDWRGLLASLPEADRAEAAADQDGPPPALAQVWASGFLAAV
                     DHWADDWAPPRDKEIAEMMSDALACVAELTDDDTGTPVLNLYDENGPPSVSQARFDAF
                     GEALWAVYDLHDIAKSLGPRVAPAVSDKVGRNDPCPCGSGKKYKKCCGQ"
     gene            complement(5672..6577)
                     /locus_tag="PKV30_01935"
     CDS             complement(5672..6577)
                     /locus_tag="PKV30_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL29011.1"
                     /translation="MSPPARALSTAAFATLLLIAALMGANHVAARLAFNHGVDVPTAV
                     SFRSGATALVVGALLLIQRVPLALTARQRKALPLIGVLIAIQSLCLYSAVARLPVALA
                     LLVFNSFPLMTALWARVLYRHRPERALLIAMPVILLGLVLALDVLGAASGLGAAGQWR
                     EIGAGVAFALGASTTFGLALVLTQHEAGGLDGRLRTFATMAMVALLAGAAVVWRGGLA
                     LPVAPAGWWGLALLTLLYGTGITILFTVLPRLGVVGNSAVMNVEPVFALVLAWLILDQ
                     HIAPVQVVGALVVVGAVIWLGTRRK"
     gene            complement(6574..7563)
                     /locus_tag="PKV30_01940"
     CDS             complement(6574..7563)
                     /locus_tag="PKV30_01940"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRL29012.1"
                     /translation="MNQKIAIYGAGAIGGWMGAKLAAVGCNVSVVARAAALDALRAHG
                     LRLREGDAETTCPVQASDDPAALGVQDLVVVSVKAPGMADVARRIGPLIGPRTIVLTA
                     MNGVPWWFLQGFGGPVAGRTLESVDPGGAIAQAIPAAQVIGCVVHASCSVDAPGVIRH
                     RAGNGLIVGEPAGGETPRVKALTDLLMQAGFNVTLSPQIQRDVWYKLWGNMTVNPVCA
                     MTGATSDRALDDELVRGFISAVMLEAKAIGARIGIPIAEQPEDRHQVTRKLGAMRPSM
                     LQDVEAGKPVEIDALVSAVRELGQLTQVPMPFTDALLGLSRLHARVRGLYPDA"
     gene            complement(7602..8387)
                     /locus_tag="PKV30_01945"
     CDS             complement(7602..8387)
                     /locus_tag="PKV30_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRL29013.1"
                     /translation="MNAPLSLRADAPPGMHMDEWAARQQLAACYRIFALLGWTEMIYN
                     HITLRVPDSATGGDKQFLINPFGLHYNEVTASNLVKVNLAGEVLGGTPHRINPAGFVL
                     HSTIHGGVGGAHCVMHTHTTAGVAVASLASGLSQSNFYSAQLHGMVATHDFEGITIHA
                     EEGPRVLASIGSKKAVILRNHGLLSWGPTLPLAFAVLWTLQRACEIQLATQSMGAPLP
                     VPEDIAAKCTRDALQFDPHHGGGQDVFDALVRQIDRLDPSYKN"
     gene            complement(8396..9391)
                     /locus_tag="PKV30_01950"
     CDS             complement(8396..9391)
                     /locus_tag="PKV30_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HRL29014.1"
                     /translation="MNPSFCRRRMVQATGAAALLASLGQRVAAQAGVETLKFVTGFAA
                     GGTSDTICRRVATRMSPGYAKQAVVENRTGAGGQIAIQYVKGQPADGATLLQTPTSML
                     TIYPHIYKKLPYDPLVDLTPVTLACVFDFGFAVGPAVPASVTSVPQFLEWAKANPQGA
                     NYGSPAAGSTPHFIGALLGKNAGVELKHAAYRGTQPAMLDLLGGNISAVSGPIGDITQ
                     HLSTGKVRILGVSGTKRSRFAPNVPTFEEQGVKGASHSEWFAFLLPPKANADVVARMN
                     AALKTALAAPDVVEGLAGFGLEAMSSSPAELAELLKRDTAKWGPIVKQIGFTAEG"
     gene            complement(9428..11158)
                     /gene="ilvD"
                     /locus_tag="PKV30_01955"
     CDS             complement(9428..11158)
                     /gene="ilvD"
                     /locus_tag="PKV30_01955"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRL29015.1"
                     /translation="MTDDTKKYALRSPTVHQGTIRASARAFLHALGQDDDDIARPHIG
                     VFHTGGEMSPCNLNLQDQAQHAKMGIYAGGGTPHECPVVSVSDGLTMAHSGMRFSLIS
                     RELIADSVEASVRGHQWDGIFGIGACDKNLPGLMMAMVRCNVPSVFVHGGSAMPGRMP
                     GAHGADLNIVDTYETLGRLLAGEATREQLDIVTRACLPTAGACAGQFTANTMGMVSEA
                     LGLAPIGSSMVPAVYSERAPLLRRAGKALMQAVLGGGPLPRDIVTREAIENACALVCA
                     TGGSTNAALHLPAIAHEAGIRFHLDDVAAVFARTPLIADLKPGGQYLARDVHSIGGAG
                     VILRALLDQGLLHGDALTFTGRTLAQELADCGLPDGEVVRRAGEPLSADGGLVVLKGN
                     LCPDGALLKTAGLKTLAHRGPARVFENEEQAQAAVQAMRYQPGEVLVIRNEGPKGSPG
                     MREMLGITALLYGQGMGDKVALLTDGRFSGATRGLCIGYAGPEAADGGPIAALRDGDV
                     IDIDARPSARRIAVELTADQIAQRLAERSAADPRRLGGLLEKYALTVRPAHQGAVTHS
                     GAVDWPRDEP"
     gene            complement(11163..11966)
                     /locus_tag="PKV30_01960"
     CDS             complement(11163..11966)
                     /locus_tag="PKV30_01960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL29016.1"
                     /translation="MPPFGVGCGVTLSARVCCITGAATGIGAACARAFAAQGWNVALS
                     AFGDEQTPLAVALAQECTAHGVQASTHALDVRDDASCAAFAQAALARHGRIDALVHCA
                     ATTRFIPHDDFDTLDAAEFHRTYDVNTVGAWRMVRALRPALHASGQGSVVLVSSIGGL
                     LGRGSSVAYAASKGALNTLTLALARAMAPAVRVNAVAPGFVAGGLPSRVLTPEQHADV
                     ERVQTEAAVLRRVSQPAEVAALALFITDKAPAMTGQVVAMDNGLHLNAG"
     gene            complement(12042..12872)
                     /locus_tag="PKV30_01965"
     CDS             complement(12042..12872)
                     /locus_tag="PKV30_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HRL29017.1"
                     /translation="METPRNPFKQALQEGRTQLGLWVALANAYSTELIAGIGYDWLLI
                     DGEHAPNDVRSVLAQLQAVASAQQAFGDLRSHPVVRVPLGTGEAGTALIKQFLDTGAQ
                     TILVPMVDTAEQARQVAAACRYPPEGVRGMGSALARASRWQAHPRYVQEANAQICCLV
                     QAETVTAMQNLDAIATTPGVDGVFIGPADLSASMGHPGNPMHPDVQAMIAQAIARILK
                     AGKAPGILSTSEAQARQWLAAGARFVAVGADTIVLVNGATQLLKTFRADTAAQGSSSY
                     "
     gene            complement(12885..13700)
                     /gene="hpaH"
                     /locus_tag="PKV30_01970"
     CDS             complement(12885..13700)
                     /gene="hpaH"
                     /locus_tag="PKV30_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HRL29018.1"
                     /translation="MLTPEQIDQLAAELQQSEATRTAVEHFSRRHPGMTIEDGYRISR
                     AWVARQLAQGRQVIGHKIGLTSRAMQLSSQIDEPDYGTLLDSMLFTCTPGEVLEIPAS
                     RFIAPRVEVELAFVLKRDLRGPHVDVEQVLHATDHVTPAIEIIDARIEQFDRHTKAMR
                     KVFDTISDNAANAGIVVGAGRADARAISRPWVGAILRCNGVVEETGLAAGVQGDPAIG
                     IAWLANKLAPWGESLQAGQIVLAGSFTRPVAARVGDVFEADYGPLGQMTFKFV"
     gene            complement(13704..14162)
                     /locus_tag="PKV30_01975"
     CDS             complement(13704..14162)
                     /locus_tag="PKV30_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HRL29019.1"
                     /translation="MPHLVILYTGNLDAIVDMPALCRELADTMLAVRDDDGAQVFPTG
                     GTRVLAYPAPHFAVADGGAAGRAAGGGKTGVSSADPGEYGFMYLHLRMGRGRSHATQN
                     RAGSALLACARGRLDAEMATRHIGLTLQIDEGAEVFDAKHSTLHPLFKKA"
     gene            complement(14167..15090)
                     /gene="hpaD"
                     /locus_tag="PKV30_01980"
     CDS             complement(14167..15090)
                     /gene="hpaD"
                     /locus_tag="PKV30_01980"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HRL29020.1"
                     /translation="MGQLALAAKITHVPSLYLSELPGDRHGTRQDAIDGHREIGRRCR
                     ELGVDTLVVFDTHWLVNANYHLNCAPHWQGVYTSNELPHFISNLPFAFPGNPELGRIL
                     AKVCNEQGVETLAHDATTLAPEYGTLVPMRYMNADQHFKVVSVSAMCTSHYLNDSARL
                     GWAMREAVEKHYDGTVAFLASGSLSHRFAQNGLAPEFAFKCWSPFLERLDADVVQMWQ
                     HGEWADFCAMLPEYAAKGHGEGFMHDTAMLLGALGWSAYDGQAEVVTPYFAASGTGQI
                     NAVFPVTKQDGQAVPVPEASRASSYKAFPRL"
     gene            complement(15113..16120)
                     /locus_tag="PKV30_01985"
     CDS             complement(15113..16120)
                     /locus_tag="PKV30_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29021.1"
                     /translation="MPTTSPITRWSRRHTLLAGAALLSGSARATDAAWPGGPVVIHTP
                     YGAGGTSHNLSGVVAAQMSISLGNEVSVQARPDQTLTSMAQAVATAPPDGRTALLVSN
                     VFVRAAVASPSVGYEPFNDFRPVGLIARSELALVTHPRTGVRSLSQMLGAARQHLRTL
                     RAASFGKDSTSDGALAQLRQLADVDMQVNHFQGQAPAVEALLAGEQDLVFINLPELQP
                     LLRQHRVVPLAIAARSRSTRLPQVPTLTELGYPIGFAPWYGLVVPAQTPDAVVEAFNR
                     ELNAAIGASVVNHVLLAAGVRPEPGTPARLREAMQDEVTLFRVQSRALDARNSAPARK
                     P"
     gene            complement(16152..17111)
                     /locus_tag="PKV30_01990"
     CDS             complement(16152..17111)
                     /locus_tag="PKV30_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29022.1"
                     /translation="MHRRHLVAASAALAFPLLGRAQAWPAKPIRVVVPFTPGGTTDFV
                     TRLVMVEVAKSLGQTIVVENKPGAGTVIGVDSVAKSAPDGHAFVTVANSFTVNQTLVK
                     KLPYDTLKDLRPVALMGLSEHVLATHPASGLKTVADIVRQAKAGQKLSYASFGNGTSA
                     HLAGEMLKSQLGIDIVHVPYKGQAPALADLLGGQVSMMFGNWPEFRGHVQSGKLVAIG
                     MATAQRSVYAPGIPTLAEQGVKIESNSWNGLLAPAATPDAIVQRMNAAVNEAMKLPAV
                     ADAFQQGGIAARSGTPAQFANFIASEIRRYAEVIRAAGIQAEN"
     gene            complement(17114..>17307)
                     /locus_tag="PKV30_01995"
     CDS             complement(17114..>17307)
                     /locus_tag="PKV30_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL29023.1"
                     /translation="AAAIEAGMCFVNSQNVRDLRQPFGGTKASGTGREGGTWSYEVFL
                     EPKNVAVSMGSHHIPHWGV"
BASE COUNT         2670 a         6062 c         5924 g         2651 t
ORIGIN      
        1 gccggccaac tgaccaagat ggtcaaccag atctgcattg ccgggctggt gcagggcctg
       61 tctgaggcca tcgcctttgg ccagcgcgcc gggcttgaca tgaatcaggc gctgggcgtc
      121 atcggcaagg gcgcggccca gagctggcaa ctcgacagcc gcggcccgac catggtcgag
      181 ggcaagtttg acttcggctt tgccgtcgac tggatgcgca aggacctggg cctggtgctt
      241 gatgaagcca agcgcaacgg cgcgcgcctg ccggtgacgg cgctggtcga ccagttctat
      301 gccgacgtgc aggacatggg cggcggccgc tgggacacgt ccagcctcat caaaaggttg
      361 aagtagcccc ttgagtcggc ctgcggcggt tgccgctgtg cccgagcgct gcgccctatt
      421 tcatcggcgg taaaggctgc acctgaatgg gctgcggcgc gttgacgatg gtcggctgcg
      481 tcacggggtg cgtttcgaca ccaggcgtcg gcgatgaggc ggcgctgccg ccggtggtct
      541 gcaccgccgt cgccgcagcc ggagccgggc tggcaccacc cgccatcgtc ggatcggcga
      601 tcagatcgat cacgcgcacc acgcgctgca cgccgtcggt ctggcgcgtc acctcggtgg
      661 ccagggtcgc ttcctgcgcg gtcaggcggc ccatcaggta agtggtgccg cgctcggtca
      721 tgacgcggat gctgcccgaa ggcacgccgt tctggttgat caggcgcgtg cgcaccttgc
      781 tcgtgatcag gctgtcgttg gagcgctgca gcggaccgga ttcaggcatc accgccagtt
      841 cgttgaccac gccttgcacg cccggcaggg cgcggatgcg cgattccacc aactggcggt
      901 cctgcgcggt cggcacttcg ccggtcacca gcaccttgcg gtaaaagctg gtcaggttga
      961 cgtggccgcg ccccggcagc gcctgcacga tggcctcgtt ggccgcgttt tcgatggtgc
     1021 ggtcggcctc ctgcgtctcg ttgctgcggc gctcggatgc catcagcaat gtgccggcgc
     1081 ccacggcgcc cccgaccagc gccaccggca cacagccagc cagcgcagca ccggccgcca
     1141 gtgccaacgc gacacgcgcg gccatccgat tcttggtcat catgaaatcc atcagggact
     1201 ctcctcgtca cccagaagtt gcaggtcgag gccttcgcac aggcagtgca gcaccagctg
     1261 gtgcgcctcg tgaatgcgcg gcgtacgctc gtgcggcacg cacacgtgga catcggtatc
     1321 gcgcagcaaa tggcccagtt cgccgccgcc gcggccggtg agggcgatca cgctcatctc
     1381 gcgctcgtgc gcggcctgcg cggcgcgtac caggttggtg gcattgccgt tggacgacag
     1441 caccagcagc acatcgcccg cgctgccgag cgcgcgcacc tgggccgcaa aagcgtcggc
     1501 gtattcgccg cgccgcgcca ccgccgtgag cgtggcggca tcggtgccca gcgccagcgc
     1561 gggcagctcg gggcgcgggc gctccagccc gcccaccagc agcgcggcgg cgtactgggc
     1621 cagcgcgccc gaagcgccgt tgccgcaaat caggatcttg ccgccgcccg tgacgcaggc
     1681 cagcagcgcc tgcgtggcat cgacaatcgg gcgcgtcagg ctttgcgccc actggtactt
     1741 caggtcggcg ctgtcgatga actgctgttg aatgtgttgc tcgagcatcg ccgccgatca
     1801 tagcgccgat tgaggcggtc agtgcaaatt taggagcagg acaagctcca cctgtgcgcc
     1861 tgcggcgcct tccccctgag ggggacaaca ccagtggccg gtgcaagccc ggccacggtg
     1921 ttctggcatc gcctgctccg cggcgggtct gatcaagctg cgtcgaaggc ggccttcagc
     1981 cattcgatgt ggtcgccgtc gatggcgacg acgtcgaagc ggcacggcgg cgggcttcgg
     2041 tggcgcatca ggtagtggcg cgcggccagc acgatgcggc gctgcttgac gccgcccacg
     2101 ctggccgccg cgccgccgtg cgtggtgccg gcgcgggcgc gcacttcgac gaacaccagc
     2161 gtaccgccgg cctcgcgcat caccagatcg atctcgccgc cgccgcgccc gggcgtccga
     2221 taattgcgct cgaccagcgt cagccccgcc gccagcaaat gggccagcgc cgcgtcttcg
     2281 gcggcgttgc cgcgcaggcg cgtggtgacg tcggtttgct tcagattaaa tagcgccacg
     2341 cgcttgtcct tcctgcgtca gcagcccaaa caatgcccaa caccgtcgcc gcgctgggtt
     2401 tccccagcta ccacctcgcg ctggatgccg cgcgcgcggc ggcgggtgcg cagcattatc
     2461 cggccggtgc gctgtacgtc gtcgccacgc ccatcggcaa cctggccgac gtcacgctgc
     2521 gcgcgctgca cgtgttggcc atcgccgacg ccatcgcctg cgaagacacg cgccgcacgc
     2581 agcaactgct gcgcagctac ggcatcgacg gccggccgct gatcgccctg cacgaacaca
     2641 acgaggccga agcggcgcaa ggcgtgatcg cgcgcctgca ggccggcgag cgcatcgcct
     2701 acgtcagcga cgccggcacg cccgccgtca gcgaccccgg cgcgcgtctg gtggcggcgt
     2761 gcgcaacggc cggcctgcgc gtgctgccgc tgcccggggc cagcagcgtg accacggcga
     2821 tcagcgcggc cggattgtcg ggcgacggtc gcttcgtctt cgccggcttt ctgcccaaca
     2881 aggccggcga gcgccaaacc gcggtcgatg cgctggcgca agagccgcgc gccgtggtgc
     2941 tgctggaggc gccgcaccgc atcgccgtgc tgggcgatgc gctggcgcgg ctgggcgagc
     3001 gcccggtgac cgtcggccgc gagctgacca agcagttcga gcagatcgcc accctgccct
     3061 gcgccgaact gcccgcctgg ctggccgccg atgcgcagcg ccagcgcggc gaattcgccc
     3121 tcgtgctgca ccccgcgccc ggcgccgacg acagcgccga gagcgaacgc gtgctgcgcc
     3181 tgctgctggc cgagttgccc accaaaagcg ctgtgaagct ggccgcagac atcaccggcg
     3241 cgccgcgcaa cgcgctgtat gagcgcgcgc tggccttgaa gtcggccgat cagtaattca
     3301 atgaaaaacg gctctggcgc ttgtccatca agcgcaaaca gctatctttt tgatggcaac
     3361 aaagattcaa cgaaagaacg catacgccac cgccaccgcc gccaccagcc ccaccgcatc
     3421 ggccaacagc gcacacggca gcgcgtggcg cgtgtgcttg acaccgacgc tgccgaagta
     3481 caccgccagc acgtagaagg tggtttcggt cgagccctgg atgatggcgg ccagcttggc
     3541 ggggaacgaa tcgacgccgt gggtctgcat cacgtccacc atcaggccgc gcgcgccgga
     3601 gccgctcaga atcttcatca gacccaccgg cacggcgggc agaaagtcgg tcggcaaacc
     3661 aagcgccgac acggcggcgg cgatcccgcc catcagcacg tccatcaaac ctgcggcgcg
     3721 gaacaccgca atggccacca gcatcgccac caggtacgga atgatctgca ccgcgacgcc
     3781 aaagccgtcc ttggcgccat cgacaaaggc gtcgtacaca gccacgccgc gcagcgcgcc
     3841 ggccaccaga aacgccgcca ccacggccag gatgaagccg gcgccgatca cgcccatcca
     3901 ctgcgcggcc tgatcgggcg ggaagcgcga cagccaccac accagcgcgc cgaccacggc
     3961 accaaaaccc gccagcggca gcaacaggcc ggcgcgccag agcggaatgc gctgcaccgc
     4021 cgccaccgct atcaaggccg acagcagcga gcaagccgtg gccaccaacg tgggcagaaa
     4081 gatgtcggcc gcgttgaagc cgaccagccc ttgcttgacc gccatggtct ggcggatggc
     4141 gatgaccgag gtggggatca gcgtgaggcc cgccgtgttc agcaccagaa acatgatctg
     4201 cgcgttggtg gcggtgtcgg gccggtcgtt gagcgtctgc aactcctgca tcgccttcag
     4261 ccccagcggc gtggcggcgt tgtccagccc gagcaggttg gcgctgatgt tcatggtcat
     4321 cgcgccctgc gccgggtgac ccgccggcac gccggggaac aggtgacgaa acaccgggtt
     4381 cacgccgcgc gcaaaggcat cgaccatgcc ggcgcgctcg ccaattttca tcaagcccag
     4441 ccacagcgcc atgatgccga ccagcccgat ggagatgtcg aagccggtct tggcggcgtc
     4501 gaacatggcg ctgagcaact tggggaagat cgtcagatcg cccagcgcca gatgcacggc
     4561 ggcggcgaca aaggcgctca ggaagaagcc gagccagacg aagttcagaa tcaaatcggg
     4621 ctccagcgct tgtctgtcaa gcgcaagcag ctatggataa cggagtatcc tgagcttttc
     4681 actgaaggcc gcggagcagg ccatgcgcgg gcacccgcgg ccgggcttgc accggccgct
     4741 gggtgcgccc cttgaggggg tggcggaaca tcgcgcagcg agtggagcct ggggtggtca
     4801 ctgtccacaa catttcttgt acttctttcc gcttccgcac gggcacgggt cgttgcggcc
     4861 caccttgtcg ctcaccgccg gggccacgcg cgggcccagg cttttggcga tgtcgtgcag
     4921 gtcgtacacc gcccacagcg cctcgccaaa ggcgtcgaag cgcgcctggc tgacgctggg
     4981 cgggccgttt tcgtcgtaca ggttgagcac tggcgtgccg gtgtcgtcgt cggtcagttc
     5041 cgcgacgcag gccagcgcat cgctcatcat ctcggcaatt tccttgtcgc gcggcggcgc
     5101 ccagtcgtcg gcccagtggt cgacggcggc cagaaagccg ctggcccaca cctgcgccag
     5161 cgcgggcggg gggccgtcct gatccgcagc ggcctcggcg cggtcggctt cgggcaggct
     5221 ggccagcagg ccgcgccagt ccatcacggc ggggctgagg gcgcgctcgt cttccagcga
     5281 atcgacctcg gcgttcaagg cggcgcgcag ctgcgcttcg cgcgccagcc agcccatgct
     5341 gaactgcgtg cgctcggcgg cgctgccgaa tacgtcgtca ccgtcgcggc cgaagatgaa
     5401 tggcagccat tcgtcttcgc caatcgcgcg gcgcgtgcac agcagcgcgg tcagcacgcc
     5461 ttcgcagaac tcccactgcg gcgtattggg gtggcgctcg cgcacggcgt cgagcacgtc
     5521 ttccagcgtg tcgagcgtgg cgctggtggc gtcgtcaagg tcggtgtcgt tgtcggcggc
     5581 gggggtggtc gaatcggtca tggcggcaat cgtggcgtga tggcagcgat ggtagccgtc
     5641 tggccgcgtg gatcgccgac cacgcagcgg atcactttcg ccttgtgccc agccagatca
     5701 ccgcgcctac gacaacgagc gcgccgacca cctgcaccgg cgcgatgtgc tggtcgagga
     5761 tcagccacgc cagcaccagc gcaaacaccg gctcgacgtt catcaccgcc gaattgccga
     5821 ccacgcccag gcgcggcagc acggtgaaca agatggtgat gccggtgccg tacagcagcg
     5881 tgagcagcgc cagcccccac cagccggccg gcgccactgg caacgccaag ccgccgcgcc
     5941 acaccaccgc cgcgccggcc agcaaggcca ccatggccat ggtggcaaag gtgcgcaggc
     6001 gcccatccag accgccggct tcgtgctgcg tcagcaccag cgccaagccg aaggtggtgg
     6061 aggcccccag cgcaaatgcc acgccggcgc cgatctcgcg ccactgcccc gccgcgccca
     6121 aacccgaagc ggcgcccagc acgtcgagcg ccagcaccag cccgagcagg atcaccggca
     6181 tggcgatcag cagtgcgcgc tccggccggt gccgatacaa cacacgcgcc cacagcgccg
     6241 tcatgagcgg aaagctgttg aacaccagca gcgccagcgc caccggcagc cgcgccacgg
     6301 ccgaatacag gcacaggctc tggatggcga tcagcacgcc gatcagcggc agcgccttgc
     6361 gttgccgcgc cgtcaacgcc agcggcacgc gctggatcag cagcagcgca cccaccacca
     6421 gcgccgtcgc cccgctgcga aagctgacgg ccgtcggcac atcaacgcca tggttgaagg
     6481 ccaggcgcgc cgccacgtgg ttggcgccca tcagcgcggc gatgagcagc agcgtggcga
     6541 aggcggcggt gctgagggcg cgcgcaggtg ggctcatgcg tcggggtaca ggccgcgcac
     6601 gcgcgcatgc aggcgcgaca ggcccagcag cgcatcggtg aacggcatcg gcacctgcgt
     6661 gagttgcccc agctcgcgca ccgccgacac cagcgcgtcg atctccaccg gcttgcccgc
     6721 ctccacgtcc tgcagcatcg acggccgcat ggcgcccagc ttgcgcgtca cctgatggcg
     6781 gtcttcgggt tgctcggcga tggggatgcc gatgcgcgcg ccgatggcct tggcctccag
     6841 catcacggcg ctgatgaagc cgcgcaccag ctcgtcgtcc agcgcgcggt cggaggtggc
     6901 cccggtcatc gcgcacaccg ggttcaccgt catgttgccc cacagcttgt accagacgtc
     6961 gcgctggatc tgcggcgaca gcgtgacgtt gaagccggcc tgcatcagca aatcggtcag
     7021 cgccttcacg cgcggtgttt cgccgcccgc tggctcgccc acgatcaggc cgttgcccgc
     7081 gcggtgccga atcacgcccg gcgcatcgac cgagcaactg gcatgcacca cgcagccgat
     7141 gacttgcgcc gccggaatgg cctgcgcgat ggcgccgccg gggtcgaccg attccagcgt
     7201 gcgccctgcc accgggccgc caaagccctg cagaaaccac catggcacgc cgttcatggc
     7261 tgtgagcacg atggtccggg ggccgatcag cgggccgatg cggcgcgcca catccgccat
     7321 acccggcgcc ttgaccgaga ccacgacgag gtcctgcacg cccagcgcgg cgggatcgtc
     7381 gctggcctgc accgggcagg tcgtttcggc atcgccctcg cgcaggcgca ggccgtgcgc
     7441 gcgcagcgca tcgagcgccg cggcgcgcgc caccacgctg acgttgcagc cgacggccgc
     7501 cagcttggcg cccatccagc cgccgatggc gccggcgccg tagattgcta ttttttggtt
     7561 caatcaagcc tccagcgctt gtccagactg cgcaagcagc ttcaattttt atagctcgga
     7621 tcgagccggt cgatctgccg caccagggcg tcgaacacgt cctggccgcc gccgtggtgc
     7681 gggtcgaact gcaacgcatc gcgcgtgcac ttggcggcaa tgtcttccgg caccggcagc
     7741 ggcgcgccca tgctttgcgt ggccagctga atctcgcacg cgcgctgcag tgtccacagc
     7801 accgcaaacg ccagcggcag cgtggggccc cacgacagca gaccgtggtt gcgcaaaatc
     7861 accgccttct tgctgccgat ggacgccagc acgcgcgggc cttcttcggc gtggatggtg
     7921 atgccctcga aatcgtgcgt ggccaccatg ccgtgcaact gcgcgctgta gaagttgctc
     7981 tgactcaggc ccgaggccag cgacgccacc gccacgcccg ccgtggtgtg cgtgtgcatc
     8041 acgcagtgcg caccgcccac gccgccgtgg atcgtgctgt gcagcacgaa gccggcgggg
     8101 ttgatgcggt gcggcgtgcc gccgagcacc tcgccggcca ggttcacctt caccaggttg
     8161 ctggccgtca cttcgttgta gtgcaggccg aacgggttga tgaggaactg cttgtcgcca
     8221 cccgttgcgc tgtccggcac gcgcagcgtg atgtggttgt agatcatctc cgtccagccg
     8281 agcagggcaa agatgcggta gcaggcggcg agctgctggc gcgccgccca ctcgtccatg
     8341 tgcatgccgg gcggtgcatc tgcccgcagg gacaatggcg cgttcatggg ctgcctcagc
     8401 cttcggcggt gaagccgatt tgcttgacga tggggcccca cttggcggtg tcgcgcttga
     8461 gcagttcggc cagctcggcg ggtgacgacg acatcgcctc cagcccgaag ccggccaggc
     8521 cttcaacaac gtcgggcgcg gccagcgcgg tcttcagcgc ggcgttcatg cgcgccacca
     8581 cgtcggcatt ggccttgggc ggcagcagga aggcgaacca ttcgctgtgc gacgcgcctt
     8641 tcacgccctg ctcctcaaac gtgggcacgt tgggcgcaaa gcggctgcgc ttggtgcccg
     8701 acactcccag gatgcgcacc ttgccggtgg acaggtgctg cgtaatgtcg ccaatcgggc
     8761 ccgacacggc cgagatgttg ccgcccagca gatccagcat ggccggctgc gtgccgcggt
     8821 aggcggcgtg cttcagctcg acgccggcgt tcttgcccag cagcgcgccg atgaagtgcg
     8881 gcgtcgaacc ggccgccggc gaaccgtagt tggcgccctg cgggttggcc ttggcccatt
     8941 cgaggaactg cggcacgctg gtcacgctgg ccggcacggc ggggccgacg gcaaagccga
     9001 agtcgaacac gcaggccagc gtcaccggcg tgaggtcgac cagcgggtcg tacggcagct
     9061 tcttgtagat gtgcgggtag atggtgagca tcgacgtcgg cgtctgcagc agcgtggcgc
     9121 cgtcggccgg ctggcccttg acgtactgga tggcgatctg gccgccggcg ccggtgcggt
     9181 tttccaccac cgcctgcttg gcgtagccgg ggctcatgcg cgtggcgacg cggcggcaga
     9241 tggtgtcgga cgtgccgccg gccgcaaagc cggtgacgaa cttgagcgtt tcaacgccgg
     9301 cctgcgcggc cacgcgctgg cccaggctgg cgagcagcgc ggcggcgcca gtggcctgca
     9361 ccatgcggcg gcggcaaaag gatgggttca tgcatgtctc ctgtgcgttg tggatgaaca
     9421 aagaaattca aggctcgtcg cgcggccagt cgaccgcgcc cgagtgggtg accgcgccct
     9481 ggtgcgccgg gcgcacggtg agggcgtatt tttccagcaa cccgcccagg cggcgcgggt
     9541 cggcggccga gcgttcggcc agtcgctgcg cgatctggtc ggccgtcagc tccacggcga
     9601 tgcgccgcgc cgacggccgg gcatcgatgt cgatcacatc gccatcgcgc agcgcggcga
     9661 tggggccgcc gtcggccgcc tccggccccg cgtagccgat gcacagaccg cgcgtggcgc
     9721 cggaaaagcg cccgtcggtc aagagcgcca ccttgtcgcc catgccctgg ccgtacagca
     9781 gggccgtgat gcccagcatc tcgcgcatgc cggggctgcc cttagggcct tcgttgcgga
     9841 tcaccagcac ctcgcccggc tggtaccgca tggcctgcac cgccgcctgc gcctgctcct
     9901 cgttctcgaa cacgcgcgcc gggccgcggt gcgccagcgt cttcaagccc gcggtcttga
     9961 gcagcgcgcc atcggggcac aggttgccct tgagcaccac cagcccgccg tcggcactga
    10021 gcggctcgcc agcgcggcgc accacttcgc cgtcgggcag gccgcagtcg gccagctcct
    10081 gcgccagcgt gcggccggtg aaggtgaggg catcgccgtg cagcaagccc tgatcgagca
    10141 gcgcgcgcaa gatgacgccg gcgccgccaa tcgagtgcac atcgcgcgcc agatactggc
    10201 cgccgggctt caggtcagcg atgagcggcg tgcgcgcgaa cacggccgcc acgtcgtcga
    10261 ggtgaaaccg gatgcccgcc tcgtgcgcga tggccggcag atgcagcgcg gcgttggtgg
    10321 agccgccggt ggcgcagacc agcgcgcagg cgttctcgat cgcttcgcgc gtgacgatgt
    10381 cgcgcggcag cgggccgccg cccagcaccg cctgcatcag cgccttgccg gcacggcgca
    10441 gcagcggcgc gcgttcgctg tacacggcag gcaccatgct ggagccgatc ggcgccaggc
    10501 ccagcgcctc gctcaccatg cccatggtgt tggcggtgaa ctggccggca caagcgccgg
    10561 ccgtgggcag gcaggcgcgc gtgacgatgt cgagctgctc gcgcgtggcc tcgccggcca
    10621 gcagcctgcc caacgtctca tacgtgtcga cgatgttcag atcggccccg tgcgcgccag
    10681 gcatgcgccc gggcatggcc gagccgccgt gcacgaacac gctgggcacg ttgcagcgca
    10741 ccatggccat catcaggccg ggcaggttct tgtcgcaggc gccgataccg aagatgccat
    10801 cccactggtg gccgcgcacc gaggcttcca cactgtcggc aatcagctcg cgcgagatga
    10861 gcgaaaagcg catgcccgaa tgcgccatgg tcaacccgtc gctcaccgac accaccgggc
    10921 attcgtgcgg cgtgccgccg cccgcgtaga tgcccatctt ggcgtgctgc gcctggtcct
    10981 gcaggttgag gttgcagggg ctcatctcgc ccccggtatg aaacacgccg atgtgcgggc
    11041 gcgcgatgtc gtcgtcgtcc tgccccagcg cgtgcaggaa ggcgcgcgcg ctggcgcgga
    11101 tggtgccctg gtgcaccgtg ggcgagcgca gggcgtattt cttggtgtcg tcggtcatga
    11161 gctcagccgg cgttgaggtg caggccattg tccatggcca ccacctggcc cgtcattgcg
    11221 ggggctttgt cggtgatgaa cagcgccagc gctgccactt ccgccggctg cgatacgcgg
    11281 cgcagcacgg cggcttcggt ctgtacgcgc tccacgtccg catgctgctc gggtgtcagc
    11341 acgcggctcg gcagaccgcc agcgacaaaa ccgggcgcca cggcgttcac gcgcaccgcc
    11401 ggcgccatgg cgcgcgccag cgccagcgtg agggtgttga gcgccccttt ggacgccgca
    11461 tacgccaccg acgagccgcg ccccagcagc ccgccaatcg acgataccag caccacgctg
    11521 ccctggccgc tggcatgcag cgccggccgc agcgcgcgca ccatgcgcca ggcgccgacc
    11581 gtgttcacgt cgtaggtgcg gtgaaattca gcggcgtcca gcgtgtcgaa atcgtcgtgc
    11641 gggatgaagc gcgtggtggc cgcgcagtgc accagcgcat caatgcggcc atggcgcgcc
    11701 agcgcggcct gcgcaaaggc ggcacaactg gcgtcgtccc gcacgtccag cgcgtgcgtg
    11761 ctggcctgca cgccgtgcgc ggtgcattct tgcgcaagcg caacggccag cggtgtctgc
    11821 tcgtcgccaa aggcgctcaa tgccacgttc cagccctgcg ccgcaaacgc gcgggcgcac
    11881 gccgcgccaa tgccagtggc ggcgccggtg atgcagcaca cgcgcgcgct gagcgtcacg
    11941 ccgcacccca cgccgaatgg cggcacatga ctttgaacac ttcgtttttc atagctgctt
    12001 gcgcagtcag gacaagcgcc agcgcccctt tttctcaagg atcagtagct cgacgagcct
    12061 tgcgccgccg tgtcggcgcg gaaggtcttg agcaactgcg tggcgccgtt gaccagcacg
    12121 atggtgtcgg cccccaccgc cacgaagcgc gcgccggccg ccagccattg acgcgcctgc
    12181 gcctcgctgg tcgacagaat gcccggcgcc ttgccggctt tcaggatgcg ggcgatggcc
    12241 tgcgcgatca tggcctgcac gtcggggtgc atcgggttgc ccgggtggcc catgctcgcg
    12301 ctcaggtcgg ccgggccgat gaacacgccg tcgacgccgg gcgtggtggc gatcgcgtcc
    12361 aggttttgca tagcggtcac cgtctcggcc tgtaccaggc agcagatctg cgcgttggcc
    12421 tcttgcacgt agcgcgggtg cgcctgccag cgcgacgccc gcgccagcgc gctgcccatg
    12481 ccgcgcacgc cctcgggcgg gtagcggcag gcggcggcca cttggcgcgc ctgctcggcc
    12541 gtgtccacca tcggcaccag aatcgtctgc gcgccggtgt cgagaaactg cttgatgagc
    12601 gccgtgccgg cctcgccggt gcccagcggc acgcgcacga ccgggtgcga acgcaagtcg
    12661 ccaaaggcct gctgcgcgct ggccacggcc tgcagctgcg ccagcacgct gcgcacgtcg
    12721 ttgggcgcat gctcgccgtc gatcagcagc cagtcgtagc cgatgccggc gatcagctcg
    12781 gtgctgtagg cgttggccag cgcgacccac aggccgagct gcgtgcggcc ctcctgcagg
    12841 gcctgcttga aggggttgcg cggtgtctcc atggcttgtc tttctcaaac gaacttgaac
    12901 gtcatctggc cgagcgggcc atagtcggct tcgaatacat cgcccaccct ggccgcgacc
    12961 ggccgcgtga aggagccggc cagcacgatc tgccccgcct gaagcgattc gccccacggc
    13021 gccagcttgt tcgccagcca ggcgatgccg atggccgggt cgccctgcac gccggcggcc
    13081 aggcccgttt cttcgaccac gccgttgcag cgcaggatgg cgccgaccca cggccggctg
    13141 atggcgcgcg cgtcggcgcg gccggcaccg accacgatgc cggcgttggc ggcgttgtcg
    13201 ctgatggtgt cgaacacctt gcgcatcgcc ttggtgtggc ggtcgaactg ctcgatgcgg
    13261 gcgtcgatga tctcgatggc gggcgtgacg tggtcggtag catgaagcac ctgctcgacg
    13321 tcaacatggg ggccgcgcag gtcgcgcttg agcacgaagg ccagttcgac ctcgacgcgc
    13381 ggcgcgatga agcggctggc gggaatctcc agtacctcgc ccggcgtgca ggtgaacagc
    13441 attgagtcca gcagcgtgcc gtagtcgggc tcgtcgatct ggctgctgag ttgcatggcg
    13501 cgcgaggtca ggccgatctt gtggccgatg acctgccgcc cctgtgccaa ctggcgcgcc
    13561 acccaggcgc ggctgatgcg gtagccgtcc tcgatggtca tgccggggtg gcgcctggag
    13621 aaatgctcga ccgccgtgcg cgtggcttcg ctctgctgca actcggcagc cagctggtcg
    13681 atctgttcgg gggtcagcat ggctcaggct ttcttgaaga gcgggtgcag cgtgctgtgc
    13741 ttggcgtcga acacttcggc gccttcgtcg atctgcagcg tgaggccgat gtggcgtgtt
    13801 gccatttcag catccaatcg gcctctggcg cacgccaaca aagcgctgcc agctctgttt
    13861 tgtgtagcat ggctgcgccc gcggcccatg cgcaggtgga gatacatgaa gccgtactcg
    13921 cccgggtcgg cgctcgacac gccggtcttg ccgccgcccg ccgcgcggcc cgccgcaccg
    13981 ccgtctgcga cagcgaaatg cggcgccggg taggccagca cgcgcgtgcc gccggtcgga
    14041 aacacctgtg cgccatcgtc atcgcgcacc gccagcatgg tgtcggccag ttcgcggcac
    14101 agcgcaggca tgtcgacgat ggcgtcgagg ttgccggtgt agaggatcac caggtgcggc
    14161 atggcgtcaa agcctgggga aggccttgta gctgctggcg cgcgaggctt cgggcaccgg
    14221 cacggcttga ccgtcctgct tggtcaccgg aaacaccgca ttgatctgcc ccgtgccgga
    14281 ggcggcgaag tacggcgtga ccacttccgc ctggccgtca tacgccgacc agccgagcgc
    14341 acccagcagc atggccgtgt cgtgcatgaa gccttcgccg tggcccttgg cggcgtactc
    14401 gggcagcatg gcgcagaagt cggcccactc gccgtgctgc cacatttgca ccacgtcggc
    14461 gtccagccgc tcgaggaaag ggctccagca cttgaaggca aattctggcg ccaagccgtt
    14521 ctgcgcaaat cggtgcgaca gcgagccgct ggccagaaaa gcgacggtgc cgtcgtaatg
    14581 cttttcaacc gcctcgcgca tggcccatcc cagtcgggcg ctatcgttaa ggtagtgact
    14641 ggtgcacatc gccgagaccg acaccacctt gaagtgctgg tcggcgttca tgtagcgcat
    14701 gggcaccagc gtgccgtatt cgggcgccag cgtggtcgcg tcatgcgcca gtgtttccac
    14761 gccctgctcg ttgcacacct ttgccaaaat gcgccccagc tcggggttgc ccgggaaggc
    14821 gaacggcagg ttgctgatga agtgcggcag ctcgttgctg gtgtacacgc cttgccagtg
    14881 cggcgcgcag ttgaggtggt agttggcgtt gaccagccag tgcgtgtcga acaccaccag
    14941 cgtgtcgacg cccagttcgc ggcagcggcg gccgatctcg cggtggccgt cgatggcgtc
    15001 ctggcgcgtg ccgtggcggt cgcccggcag ctcgctcagg tagagcgaag gcacgtgcgt
    15061 gatcttggcg gcaagggcga gttgtcccac gggcaattcc ttgatgagcg gttcacggct
    15121 tgcgcgccgg cgcgctgttg cgcgcgtcca gcgcgcgcga ctgcacgcga aacagcgtca
    15181 cctcgtcctg catggcttcg cgcaggcgcg cgggcgtgcc gggctcgggc cgcacgccgg
    15241 cggccagcag cacgtggttg acaaccgacg cgccgatggc ggcgttcagc tcgcggttga
    15301 aggcttcgac caccgcatcg ggcgtctgtg ccggcaccac cagcccgtac cacggcgcga
    15361 acccgatggg gtagcccagc tcggtcagcg tgggcacttg cggcaggcgt gtgctgcgcg
    15421 agcgggccgc aattgccagc ggcaccacgc ggtgctgtcg cagcagcggt tgcagctcgg
    15481 gcaggttgat gaacaccagg tcctgctcgc cggccagcag cgcctcgacc gccggcgcct
    15541 ggccctgaaa gtggttcacc tgcatgtcga catccgccag ctgccgcagt tgcgccagcg
    15601 cgccgtccga ggtgctgtcc ttgccaaaac tcgcggcgcg cagcgtgcgg aggtgctggc
    15661 gcgcggcgcc cagcatctgc gacagcgagc gcacaccggt gcgcgggtgc gtgaccagcg
    15721 ccagctcgga gcgcgcgatc aggccgaccg gcctgaagtc gttgaacggc tcataaccca
    15781 cggacggcga ggccaccgcc gcgcgcacga acacgttgga aaccagcagc gccgtgcgcc
    15841 catccggcgg cgcggtggcc acggcctgcg ccatgctggt cagcgtctgg tccgggcgcg
    15901 cctgcaccga cacctcgttg cccaggctga tcgacatctg cgccgccacc acgccgctca
    15961 ggttgtgcga ggtgccgccg gcgccgtacg gcgtgtgaat gaccaccggc ccgcccggcc
    16021 aggccgcatc ggtggcccgt gcgctgcccg aaagcagcgc cgcgccggcc agcagggtgt
    16081 ggcggcgcga ccagcgcgtg atgggggaag tcgtgggcat aggggccaat tcaggtgagg
    16141 ggtaacagcc ctcaattctc ggcctggatg cccgccgccc gaatcacctc ggcatagcgc
    16201 cggatttcgc tggcgatgaa gttggcaaac tgcgccggcg tgcccgatct ggcggcaatg
    16261 ccgccttgtt ggaaggcatc ggccacggca ggcagcttca tggcctcgtt caccgccgcg
    16321 ttcatgcgct gcacgatggc gtcgggcgtg gcggcgggcg ccagcaggcc gttccatgaa
    16381 ttcgattcga tcttcacgcc ctgctcggcc agcgtgggga tgccgggcgc gtacacggag
    16441 cgctgcgccg tcgccatgcc gatggcgacc agcttgcccg actgcacatg gccgcgaaac
    16501 tccggccagt tgccgaacat catcgacacc tgcccgccca gcagatcggc cagcgccggt
    16561 gcctggccct tgtagggcac gtgcacgatg tcgatgccca gttgcgactt cagcatctcg
    16621 cccgccaggt gcgccgaggt gccgttgccg aacgacgcgt agctgagctt ttgccccgcc
    16681 ttggcctggc gcacgatgtc ggccaccgtc ttcaggccgc tcgccgggtg cgtggccagc
    16741 acgtgctcgg acaggcccat cagcgccacc ggccgcagat ccttcagcgt gtcgtaaggc
    16801 agctttttca ccagcgtctg attgaccgtg aagctgttgg ccacggtgac gaaggcgtgg
    16861 ccgtccggcg ccgacttggc cacgctgtcg acgccgatca ccgtgccggc tcccggcttg
    16921 ttctcgacca cgatggtctg gcccagcgac ttggccacct ccaccatcac cagccgcgtg
    16981 acgaaatcgg tggtgccgcc gggcgtgaaa ggcacgacga cgcggatcgg cttggcaggc
    17041 caggcctggg cgcgtcccag cagcgggaag gcgagcgcgg cagaagcggc gacgaggtgg
    17101 cgacggtgca tgctcaaact ccccaatgtg gaatgtgatg ggaacccatc gagaccgcca
    17161 cgttcttggg ctccagaaac acctcgtagc tccaggtgcc gccttcgcgc ccggtgcccg
    17221 atgccttggt gccgccaaag ggctgacgca aatcgcgcac gttctggctg ttgacgaagc
    17281 acatgccggc ctcgatggcg gcggcca
//
LOCUS       DAOMVO010000018        16756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_682_length_16756_cov_15.878989, whole genome shotgun sequence.
ACCESSION   DAOMVO010000018 DAOMVO010000000
VERSION     DAOMVO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16756
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_682_length_16756_cov_15.878989"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..888
                     /locus_tag="PKV30_02000"
     CDS             256..888
                     /locus_tag="PKV30_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRL29024.1"
                     /translation="MKHIDFYLDFISPYAYLAFEALPRALQGLSYEVTYRPILFAGLL
                     KHHGQLGPAEIGPKRDWTYRQVLWQAREHGIAFDMPAAHPFNPLALLRLAWASARQGT
                     PNRYVCETIFRHVWQGGAPADDVARLEALTAQLAPARDPASDAVKHDLRAATDAALAA
                     DVFGVPTFAVDGRVFWGLDALPLLRAQLEGDTRVDEVWACAASVAQGVRR"
     gene            980..2797
                     /locus_tag="PKV30_02005"
     CDS             980..2797
                     /locus_tag="PKV30_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HRL29025.1"
                     /translation="MPNAFNFSASPFDSLTQHEQRLVRDHVDVVYFRADEVVLDVGAV
                     PAHLFVVIKGYVNQLDADELTTTYGPDDCFDGRGLVAGKASSRFVAAEEVVAYALARH
                     TVQELIAANATFGALLFSDLGAKLSAAGQRQSGNELQALNMARVDQAFVRPAHVVDAG
                     TDVVSVVRLFQQERTTNVLVQDGRQQPPRLGIFTTNALQRAILSGRPLDQIPVGELSN
                     YSLITVRPGDQVGDALAIMLRHHIHRVVVADGERIHGVLEALDVFSFLSNHSMLITLR
                     INDAADIDELAEAAAQLTGMIGRQFRSGTRVGLIARMVQDLNARLFDRAWQLIAPAEL
                     VANSCLFVMGSEGRGEQLLKTDQDNGLVLRDGYAPPADLPSICERFSDALARFGYPPC
                     PGGIMVSNPAWRMTAADFAQTARRWLAMPEADSLMNLAIFMDAHAVSGDARLLDAVKA
                     SLMQMATDSDALLARFASAIDAFGGSVGWWNRLLGDAGERLNLKKEGIFPLVHGVRSL
                     ALAGRLDATGTVERIEALVQRGDLSADMGRELTESLHFFMSLKLKAGLAELDRQQPVS
                     GAVDAKALSSFERDLLKDTLGVVKRFKAQLHHRFKMNAL"
     gene            2794..3459
                     /locus_tag="PKV30_02010"
     CDS             2794..3459
                     /locus_tag="PKV30_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL29026.1"
                     /translation="MTGWVDRLRRGWQRRQFRNSEWRFLFDAPPPGEWVALDCETTGL
                     NSRTDEIIAIGAVRIVGQRILTSERLELLVRPEGDVPAESIRVHRLRAQDVKAGLNTE
                     DAMRQLLHFIGPRPLVGYYLEFDVAMINRALKRTLGLTLPQPKIEVSALYYDHVFQQL
                     PPHRQQENTEIDLRFATIMDALGLPTRDAHDALNDAVMAALAFVKLRALRGQAATEML
                     HKK"
     gene            complement(3549..5276)
                     /locus_tag="PKV30_02015"
     CDS             complement(3549..5276)
                     /locus_tag="PKV30_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRL29027.1"
                     /translation="MSRTRLPLVAALLALAAGAVHAAGGDVGEAAKQATNWIAIIMFT
                     VFVVATLGITKWAAGRTKSAADFYTAGGGITGFQNGLAIAGDYMSAASFLGISAAVMA
                     SGYDGLIYSIGFLVGWPILTFLLAERLRNLGKFTFADVAGYRFQQTPIRAFAASGTLV
                     VVAFYLIAQMVGAGQLIKLLFGLDYWIAVVLVGALMMVYVLFGGMTATTWVQIIKAVL
                     LLSGVTFMGFMVLAKYGFSPEALFAEGVRVRSQIAANGGADPTAAAKAGLAIMGPGGF
                     IKDPVSAISFGMALMFGTLGLPHILMRFFTVPNAKEARKSVFWATTWIGYFYILIFII
                     GFGAITLVLTNPEFADTTKGIIHGGAGTANMAAVLVAKSVGGDVFYGFISAVAFATIL
                     AVVAGLTLSGASAVSHDLYSTVFKKGKADSAKELRVSRITTLALGVIAVVLGILFEKQ
                     NIAFMVSLAFAIAASANFPVLLLSVLWKDCTTKGAVIGGFLGLISSVALTIVSPSVWE
                     ATFGNPKGSALFPYTSPALFSMTIGFVGIWLFSILDKSKNAAAERAAFPAQQVRSETG
                     LGAAKASSH"
     gene            complement(5273..5581)
                     /locus_tag="PKV30_02020"
     CDS             complement(5273..5581)
                     /locus_tag="PKV30_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRL29028.1"
                     /translation="MTDPMVERIQRNPKYLQLKSTRGRYGWTLAILMLIVYYGYIALI
                     AFDKEFLAKPMGAGVTTIGIPIGMAVIVFSIVITGLYVRRANSEFDALTREIIEEATQ
                     "
     gene            5961..7634
                     /locus_tag="PKV30_02025"
     CDS             5961..7634
                     /locus_tag="PKV30_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL29029.1"
                     /translation="MATATSAAANRPMTAEEKKVIFASSLGTVFEWYDFYLYGSLAAI
                     IAKQFFAGLDPTSAFIFALFAFAAGFIVRPFGALFFGRLGDMIGRKYTFLVTILIMGL
                     STFVVGLLPNYASIGVAAPVILIVLRMLQGLALGGEYGGAATYVAEHAPHGKRGAYTS
                     WIQTTATLGLFLSLIVILVTRNVLGEDAFQAWGWRIPFLISIALLAISVWIRLSMNES
                     PAFQKMKSEGKTSKAPLTESFGEWKNLKIVLLALFGLVAGQAVVWYTGQFYALFFLQS
                     VLKVDASTANILIAIALLIGTPFFVVFGSLSDKIGRKPIIMAGLLLAVLTYFPLFKAL
                     TNAANPDLAAAQAANQVVVTANPAECSFGGNPVAREIDFRTSCDIAKRFLAQQSVSYE
                     NVAGTGPATVKIGDKLITAPAGNVVNSKFDESSAKAIAAFKKEVSDTLKAAGYPAKAD
                     PAKMNKPLIVAILTLLVIYVTMVYGPIAAALVEMFPTRIRYTSMSLPYHIGNGWFGGM
                     LPTISFAMVAANGNMYYGLWYPIVIAAITFIIGVLFVKETKDVDIYATD"
     gene            7650..7796
                     /locus_tag="PKV30_02030"
     CDS             7650..7796
                     /locus_tag="PKV30_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29030.1"
                     /translation="MWKIVIGFIIFAAIAVYVLSKGGDIDLTGEKHGALQPPASQPAL
                     TTKA"
     gene            7918..13944
                     /locus_tag="PKV30_02035"
     CDS             7918..13944
                     /locus_tag="PKV30_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRL29031.1"
                     /translation="MSSPLIRIRGARQHNLKNLDLDIRTGELTVVTGPSGSGKSSLVF
                     DTLFAEGQRRYVETFSAYARQFLDRMDKPAVDKVEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLLARAGQLFDADTALPVRHDNADSIYAEMQRRALESDDPRLVVTFAVE
                     LPASATTEEVQQWLSASGFTRVQAERESEGKKTLDVVADRFRIGSAERARVIEAIEVA
                     LKRGAGRMMVYALPNQELPALDGQAPEGDLALKSVTAPVLWKFSTGLHCPESNRRYSD
                     PIPSMFSFNSAVGACDTCRGFGRVIGVDWGLVIPDERLTLRAGAIKPIQTPAWKEAQD
                     DLMRHAEAAGIPRDTAWNKLTPEQKAWVIDGTPNYKEGNWGKQWYGIARFFKYLESKA
                     YKMHIRVLLSKYRSYTPCPDCGGARLKTEPLLWRVGTKADADAVLEPSKRFMPVGVAW
                     SREQLEALPGLCLHDLMLLPIDRLRIFFDRLGDQIATKTEAAGAYPSSAGGVFDADSA
                     DAKALALILEEITTRIKYLCDVGIGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTL
                     FVLDEPSIGLHPRDMNRITEAMQRLKSAGNTLVVVEHDPAVMLAADRVLDFGPGPGAA
                     GGRIVFDGTPDELRAADTLTGAYLGGRRHIGLGYARPVADHTPRLILEGATEHNLKQV
                     TVDFPLQRLVVITGVSGSGKSTLVQDVLSPALLRHFGKATESPGAHERLLGADHLSDV
                     VFVDQSPIGKTARSNPASYVGAWDAIRALFAQAPLAKQRGYTAAKFSFNSGDGRCATC
                     GGSGFEHVEMQFLSDIYLRCPDCDGKRYRPEILEVTLQRPVGKLGSDSNFQDAADRLA
                     QAVDGKLDADPHLPVHRKRLLNVADVLDLTVAEAAHLFAQDREVIRALQPIVDVGLDY
                     VKLGQPVPTLSGGEAQRLKLAGFLADAAQSATASRQPAARKGTLFLFDEPTTGLHFDD
                     IAKLMRAFRKLLDAGHSLIVIEHNLDVMRAADWLIDLGPEGGEAGGQVVAEGTPDEVM
                     QHTTSHTGAALRAYAQAIGAAVGVSAEEPRSLYKTELLALDTPAQEAQNSIEIVHARE
                     HNLKNLSVDIPRGKFNVITGVSGSGKSTLAFDILFNEGQRRYLESLNAYARSIVQPAG
                     RPEVDAVYGIPPTVAIEQRLSRGGRKSTVGTTTEVWHFLRLLYVKLGVQHCVHDGAAV
                     QPQTPERIAAQIMTRYRGQTIGLLAPLVVGRKGVYTELADWARPRGFTHLRVDGEFLP
                     TTNFPRIDRFKEHTIELPVMSLPVTPGNEAQLRESLTRALEHGKGVVHVLHDLAGLRN
                     AMETGQSTARIGQVEVFSTKRACPVCATSYAELDPRLFSYNSKHGWCPDCVGTGVKLT
                     REQRKALDDSVLADDQKGREQTFDEPEVEDVGDVVCPTCQGTRLNPVARAVLLEAGAM
                     DGIAITDLARLSVHDLRTWFEDLKLEGRDADIARDLIPEIKSRLDFLAQVGLGYLTLD
                     RGAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLHARDNQILLDALHTLSSKGN
                     TLVVVEHDEDTIRRAEHLIDIGPGAGVRGGRLVAEGTAADLEAAEDSATGRYLREAMR
                     HPMQARRAIESEAARADGMGAGGRNSASASEATPRWLHLLGAQLHNLKQVDVAVPLQR
                     LVAVTGVSGSGKSTLARDVLLANVAAWVGQRATKAGRDAMDAGKAPPLIGCTGLRGFE
                     SVDRVLEVDQTPIGKTPRSCPATYIGFWDTVRKLFADTLEAKARGYGPGRFSFNTGEG
                     RCPACEGQGVRTIGMSFLPDVKVPCEVCHGARFNPETLAVTWRGKSIGDVLQMEVDEA
                     VDFFASMPAIAHPLQLLKDVGLGYLTLGQPSPTLSGGEAQRIKLVTELSKVRDDVGRR
                     GQKAPHTLYVLDEPTVGLHMQDVEKLIHVLHRLVNGGHSVLVIEHDLDLIAEADWIID
                     LGPEGGDGGGQLVAAGTPEDVVRAGTHTGVALARVLQRAGGVTVAVARIK"
     gene            14066..15022
                     /locus_tag="PKV30_02040"
     CDS             14066..15022
                     /locus_tag="PKV30_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29032.1"
                     /translation="MHRRTLIALCTAVTLAGPALAQTFPTKPIKLVIAFPAGGPTDIT
                     MRALADGASKALGQPVVVENKPGAGGTLPAQALQTAPADGYTLAQIPLGVFRLPYTTK
                     INWDPVKDINYVLNVTGYAFGVVVPTDSPIKSWTHFVAWAKANPGKLTYGSTGTMTSP
                     HLTMELVAQKAGLQLTHVPYKGSADLMQAVLGGHIMAASDSTGFAPQVEAGKLRVLNT
                     WGDKRLDKFPDAPTLKELGYDIVQNSPFGIGAPRDTPPAVVKRLHDAFKTAMEQPAYL
                     DALKRYDMVPIYMNSAQYKKFAQDTFTREKALIEKLGLAKGS"
     gene            complement(15030..15857)
                     /locus_tag="PKV30_02045"
     CDS             complement(15030..15857)
                     /locus_tag="PKV30_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRL29033.1"
                     /translation="MRALVERPAGAWSKVVGLLTDIDDTLTTEGAITPDALQALADLR
                     DAGLPVIAVTGRPVGWSVPFALAWPLRAIVAENGAAALIPGDIGQIGRQRLPDQREQL
                     SKIYQQDAAMRARNFEQLQRAAARVLREVPGSALAQDSPGRETDIAIDHSEFSRLDEA
                     QIAQAVAVMQAEGLHATVSSIHINGWIGTHDKWQGACWIARELLDRDLPAEAERWVYV
                     GDSTNDQVMFEHLPQSVGVANIRRFESKLKYLPRYITRGERGAGFAEVARALLADRT"
     gene            complement(15841..>16756)
                     /locus_tag="PKV30_02050"
     CDS             complement(15841..>16756)
                     /locus_tag="PKV30_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL29034.1"
                     /translation="PQVQRIYVVDDKCPQDSGAFVQQHCTDPRVRVIFHEENQGVGGA
                     VGSGYRAAIEEGMDIVVKVDGDGQMDPALIPHFTRPIERGRADYTKGNRFYRPESLKG
                     MPPIRLFGNAALSFINKLSTGYWSIMDPTNGYTAIHTAVLREMPLHKLEKRYFFETDM
                     LYHLNTMRAVVHDVPMDAVYADEESSLKVSKVLPEFIVKHVNRFFKRYVYLYLLRDFN
                     IGSLYSLLGVVLCGLGVVFGGWAWLRSLTTGQPATSGTVMLAALPLIIGIQFLIAFLH
                     HDVERVPSQPLSEDLTDDPEETPAARAG"
BASE COUNT         2924 a         5532 c         5500 g         2800 t
ORIGIN      
        1 aacgcgccgt ctgggcgctg atcgcccagc gcagcggtgg ggcgcgggtg ctggaggagt
       61 gaacagccga acagccttac gcgaaggacg cgaagggttc gcgaaggacg cgaaaaacag
      121 ccgtcaaaat gtttgttgaa gcgcggacgc caatgcgata gaccagggtt aagtcatgag
      181 tttctttttg acttccttgt gcgtccttcg cgaacccttc gcacccttcg cgttcggctg
      241 ttttcgagcg ccaccatgaa acacatcgac ttctacctcg acttcatttc gccctacgcc
      301 tacctggcgt ttgaagcgtt gccgcgcgcg ctgcagggct tgagctacga agttacctac
      361 cgcccgattc tgtttgccgg cctgctcaag caccacggcc aactgggccc ggccgagatt
      421 ggccccaagc gtgattggac ttaccgccag gtgctgtggc aggcgcgcga gcacggcatt
      481 gcgtttgaca tgccggcggc tcacccgttc aacccgctgg cgctgctgcg cctggcttgg
      541 gcctcggcgc gacaaggcac gccgaaccgc tatgtgtgcg agaccatctt ccgccacgtg
      601 tggcaaggcg gtgcgccggc cgatgacgtg gcgcgccttg aggcgctgac ggcgcaactg
      661 gcgccagcgc gcgacccggc gagcgacgcc gtgaagcacg atttgcgcgc cgccaccgat
      721 gcggccttgg cggccgacgt gttcggcgtg cccaccttcg ccgtcgatgg ccgcgtgttc
      781 tggggcttag acgcgctgcc cttgctgcgc gcgcagctcg aaggcgacac ccgcgtggac
      841 gaagtctggg cctgcgccgc aagcgtggcg caaggcgtgc ggcgctgaat acaggggcgc
      901 ggcgggtgcc ggcgtacttt taatttgata gctgcttgcg cttgatggac aagcgctggc
      961 ggcctattct gttcaagaca tgccgaacgc cttcaacttc agcgcctcgc cgttcgacag
     1021 cctgacgcag cacgagcagc gcctggtgcg cgaccatgtg gacgtggtct atttccgcgc
     1081 ggacgaagtg gttctggacg tgggcgccgt gccggcccat ctgttcgtcg tcatcaaggg
     1141 ttacgtcaac cagctcgacg ccgacgagct gaccaccacc tacgggcccg acgactgctt
     1201 cgacggtcgc ggcctggtcg cgggcaaggc cagcagccgc ttcgtcgccg ccgaagaggt
     1261 ggtggcctac gcgctggcgc gccacacggt gcaggagctg attgccgcca acgccacctt
     1321 tggcgcgctg ctgttttccg acctgggcgc caagctgagc gccgccggcc agcgccaaag
     1381 cggcaacgag ctgcaggcgc tcaacatggc gcgcgtcgat caggcctttg tgcggccggc
     1441 gcatgtggtc gatgccggca ccgacgtggt gtccgtggtg cgcctgttcc agcaagagcg
     1501 caccaccaac gtgctggtgc aggacggccg ccagcagccg ccgcgcctgg gcatcttcac
     1561 caccaacgcg ctgcagcgcg ccattttgtc gggacggccg ctggatcaga ttccggtcgg
     1621 cgagctgagc aactattcgc tcatcaccgt tcggcccggc gaccaggtgg gcgatgcgct
     1681 ggccatcatg ctgcgccacc acatccaccg cgtggtggtg gccgatggcg aacgtatcca
     1741 cggcgtgctg gaggcgctgg acgtgttcag ctttttgtcg aaccattcga tgctgatcac
     1801 gctgcgcatc aacgacgccg ccgacatcga cgagctggcc gaggcagcgg cccaactcac
     1861 cggcatgatc ggccgccagt tccgcagcgg cacgcgcgtg ggcctgatcg cgcgcatggt
     1921 gcaagacctg aacgcgcgcc tgttcgaccg cgcctggcag ctcatcgcgc cagcggaatt
     1981 ggtggccaac agctgcctgt tcgtcatggg cagtgaaggg cgcggcgaac aattgctcaa
     2041 gaccgatcag gacaacggcc tggtgctgcg cgacggctat gcgccgccgg ccgatttgcc
     2101 aagtatttgt gagcgctttt cagacgccct ggcgcgcttt ggctacccgc cgtgcccggg
     2161 cggcatcatg gtcagcaacc ccgcctggcg catgaccgcg gccgatttcg cgcagaccgc
     2221 gcgccgctgg ctggccatgc ccgaggccga cagcctgatg aacctggcga tcttcatgga
     2281 cgcgcacgcg gtgagcggcg acgcgcgcct gctggatgcg gtcaaggcca gcctgatgca
     2341 gatggcgacc gacagcgacg ccctgctggc gcgctttgcc agcgccatcg acgcctttgg
     2401 cggcagcgtc ggctggtgga accgcctgct gggcgatgcg ggcgagcggc tgaacctgaa
     2461 gaaggaaggc atttttccgc tggtgcatgg cgtgcgcagt ctggcgctgg cggggcggct
     2521 tgacgccacc ggcaccgtcg agcgcatcga ggcgctggtg cagcggggcg atttgagcgc
     2581 cgacatgggg cgcgagctga ccgagagcct gcacttcttc atgagcctga agctcaaggc
     2641 gggcctggcc gaactcgacc gccagcagcc ggtgagcggc gccgtcgacg ccaaggcgct
     2701 gagcagcttc gagcgcgatt tgctgaagga cacgctcggc gtcgtcaagc gcttcaaggc
     2761 gcaactgcac catcgcttca agatgaacgc gctatgaccg ggtgggtgga tcggctgcgg
     2821 cgcggctggc agcggcgcca gttcaggaat tcggaatggc gctttctgtt cgatgcgccg
     2881 ccgccgggcg aatgggtggc gctcgactgc gaaaccaccg gcctgaacag ccgcaccgac
     2941 gagatcattg ccattggcgc cgtgcgcatc gtgggccagc gcatcctgac cagcgagcgg
     3001 ctggagctgc tggtgcggcc cgagggcgat gtgcctgccg agagcatccg cgtgcaccgc
     3061 ctgcgcgcgc aggacgtgaa ggccggcttg aacaccgagg acgccatgcg ccagttgctg
     3121 catttcatcg ggccgcggcc gctggtgggc tattacctcg agttcgacgt cgccatgatc
     3181 aaccgcgcgt tgaagcgcac gctggggctg acgctgccgc agcccaagat cgaggtgtcg
     3241 gccctgtact acgaccatgt gttccagcaa ctgccgccgc accggcagca ggagaacacc
     3301 gagatcgacc tgcgctttgc caccatcatg gacgcgctcg gcctgcccac gcgcgacgcg
     3361 cacgacgcgc tgaacgatgc ggtgatggcg gcgctggctt tcgtcaagct gcgggcgctg
     3421 cgcggccagg cggcgaccga aatgctacat aaaaaatagc tccttgcgct tggtattcaa
     3481 gcgctggagg ccgatttggc ataaaaaacc gggcgcacgg cccggttttc tttgcgctga
     3541 cgccgtgctc agtgcgacga cgccttcgct gcgcccagcc cggtctcgga ccgcacctgc
     3601 tgcgccggga aggcggcccg ctcggcggcg gcgttcttgc tcttgtcgag gatcgagaac
     3661 agccagatgc cgacgaagcc gatggtcatg gagaacagcg ccggcgaggt ataggggaac
     3721 agcgccgagc ccttggggtt gccgaaggtg gcttcccaca ccgagggcga cacgatggtc
     3781 agcgccaccg acgagatcag gccgaggaag ccgccgatca ccgcgccctt ggtggtgcaa
     3841 tccttccaca gcaccgacag cagcagcacc gggaagttgg ccgacgccgc gatggcaaac
     3901 gccagcgaca ccatgaaggc gatgttctgc ttctcgaaca ggatgcccag caccaccgcg
     3961 atcacgccca gcgccagcgt ggtgatgcgc gacacgcgca gttccttggc gctgtcggcc
     4021 ttgcccttct tgaacacggt cgaatacagg tcgtgcgaca ccgccgaggc gcccgacagc
     4081 gtcaggcccg cgaccaccgc caggatggtg gcgaaggcca cggccgagat gaagccgtag
     4141 aacacgtcgc cgcccacgct cttggccacc agcaccgccg ccatgttggc cgtgccggcg
     4201 ccgccgtgga tgatgccctt ggtggtgtcg gcaaactcgg ggttcgtcag caccagggtg
     4261 atggcgccaa agccgatgat gaagatcagg atgtagaagt agccgatcca ggtggtggcc
     4321 cagaacaccg acttgcgcgc ttccttggcg ttgggcacgg tgaagaagcg catcaggatg
     4381 tgcggcaggc cgagcgtgcc gaacatcagc gccatgccga agctgatggc cgacaccggg
     4441 tccttgatga agccgcccgg gcccatgatg gccaggcccg ccttggcggc ggcggtggga
     4501 tcggcgccgc cgttggccgc gatctggctg cgcacgcgca cgccttcggc aaacagcgcc
     4561 tcggggctga agccgtactt ggccagcacc atgaagccca tgaaggtgac gcccgacagc
     4621 agcagcaccg ccttgatgat ctgcacccag gtggtggccg tcatgccgcc gaacagcacg
     4681 tagaccatca tcagcgcgcc caccagcacc acggcaatcc agtagtccag gccgaacagc
     4741 agcttgatga gctggccggc gccgaccatc tgcgcgatca ggtagaacgc caccaccacc
     4801 agcgtgccgc tggcggcaaa ggcgcggatc ggcgtctgct ggaagcggta gccggccacg
     4861 tcggcaaagg tgaacttgcc caggttgcgc aggcgctcgg ccagcaggaa ggtgaggatc
     4921 ggccagccga ccagaaagcc gatggaatag atcaggccgt cgtaaccgct ggccatgacg
     4981 gccgccgaga tgccgaggaa ggacgccgcc gacatgtagt cgcccgcgat ggccagcccg
     5041 ttctgaaagc cggtgatgcc gccgccggcg gtgtagaagt cggccgcgga cttggtgcgg
     5101 ccggccgccc acttggtgat gcccagcgtg gccaccacga agacggtgaa catgatgatg
     5161 gcgatccagt tggtcgcctg cttggcggct tcgccgacgt cgccgccggc tgcgtgcacg
     5221 gcaccagcgg ccagcgccag cagcgccgcg acaaggggta ggcgggtgcg gctcattggg
     5281 tggcctcttc gatgatctcg cgcgtcagcg catcgaactc gctgttggcg cggcgcacgt
     5341 acagcccggt gatgacgatc gagaacacga tgaccgccat gccgatggga atgccgatgg
     5401 tggtcacgcc tgcgcccatg ggcttggcga gaaactcctt gtcgaacgcg atcagcgcga
     5461 tgtagccgta atagacgatc agcatcagga tcgccagcgt ccagccgtag cggccgcgcg
     5521 tgcttttcag ctgcaggtac ttggggttgc gctgtatgcg ctcgaccatg gggtcggtca
     5581 tgcttgtctc ctccagggtt ggggtttcgt tgtgcgccaa gggcgatttc ggcgcgattc
     5641 tgggcagagg cactgaccac gtgcttacgt tggcagtagg gtcatgtgca aataggaaac
     5701 aagttttttg tcggggtttt cacggagccc gcggcggcgc accgacggcc ggcgagcggt
     5761 ttaacccgcg tcaaatttca catcaaaaaa aatagcatca gggttgcccc tgaggctgtc
     5821 agtttgcgat cagccaggcg ctgccgcacg cgcccggctt gtcagaaagt cgtcagtcag
     5881 atcgaaacaa atcaacgctt ccctacacat ttgcttgctt ttcgcgggcg attttttcaa
     5941 caccttgagg agaccagtgc atggcaacag cgacatccgc ggcggccaac cgccccatga
     6001 cggcggagga gaagaaagtc atcttcgcct catcactcgg caccgtgttc gagtggtatg
     6061 acttctatct ctacggttcg ctggcagcca tcattgccaa gcagttcttc gccggccttg
     6121 acccaacgtc ggccttcatc ttcgcgctgt tcgcgtttgc cgccggcttc atcgtgcggc
     6181 cgttcggcgc gctgttcttc ggccgcctgg gcgacatgat cggacgcaag tacaccttcc
     6241 tggtcaccat cctgatcatg ggcctgtcga ccttcgtcgt cggcctgctg cccaactacg
     6301 cctcgatcgg cgttgcggcc ccggtgatcc tgatcgtgct gcgcatgctg cagggtctgg
     6361 cgctgggcgg tgaatacggc ggcgccgcca cctacgtggc cgagcacgcg ccgcacggca
     6421 agcgcggtgc gtacacgtcg tggatccaga ccacggcaac gctgggcctg ttcctgtcgc
     6481 tgatcgtcat cctggtcacg cgcaacgtgc tcggcgagga cgcgttccag gcctggggct
     6541 ggcgcattcc gttcctgatc tcgatcgcgc tgctggcgat ctcggtgtgg attcgcctgt
     6601 cgatgaacga atcgccggcg ttccagaaga tgaagtccga gggcaagacc tccaaggcgc
     6661 cgctgaccga gtccttcggc gagtggaaga acctgaagat cgtgctcctg gcgctgttcg
     6721 gcctggtcgc gggtcaggcc gtggtgtggt acacgggtca gttctacgcg ctgttcttcc
     6781 tgcagagcgt gctcaaggtc gatgcatcga ccgccaacat cctgatcgcc atcgcgctgt
     6841 tgatcggtac gccgttcttc gtcgtgttcg gctcgctgtc cgacaagatc ggccgcaagc
     6901 cgatcatcat ggctggcctg ctgctggcgg tgctgacgta tttcccgctg ttcaaggcgc
     6961 tcaccaacgc cgccaacccg gacctggccg ccgcgcaggc cgccaaccag gtcgtggtca
     7021 cggcgaaccc ggccgagtgc tcgttcggcg gcaacccggt ggcgcgtgaa atcgacttcc
     7081 gcacctcgtg cgacatcgcc aagcgcttcc tcgcgcagca atcggtgagc tacgagaacg
     7141 ttgctggcac cgggcccgca acggtgaaga ttggcgacaa gctcatcacc gcgcctgccg
     7201 gcaacgtggt caacagcaag ttcgatgaat ccagcgccaa ggccatcgcc gccttcaaga
     7261 aggaggtgag cgacacgctc aaggccgctg gctacccggc caaggccgat ccggccaaga
     7321 tgaacaagcc gctgatcgtc gccatcctga cgctgctggt gatctacgtg accatggtgt
     7381 atgggccgat tgccgccgcg ctggtcgaga tgttccccac acgcatccgc tacacctcca
     7441 tgtcgctgcc ttatcacatc ggcaacggct ggttcggcgg catgctgccc accatctcgt
     7501 tcgccatggt ggccgccaac ggcaacatgt actacggctt gtggtatccc atcgtgatcg
     7561 ccgccatcac cttcatcatt ggcgtgctgt tcgtgaagga aaccaaggac gtggacatct
     7621 acgccacgga ttgatgggag ccaaacgcca tgtggaaaat cgtcattggt ttcatcatct
     7681 ttgcggccat cgcggtctac gtgctgtcca agggcggtga catcgacctg accggagaaa
     7741 agcatggcgc gctgcagccg ccagcgtccc agccggcgtt gacgaccaag gcctgacggc
     7801 tcctcgtcaa cgcccacccc aaagcccgcc ttgtgcgggc ttttttcatg gcccgccgcg
     7861 ctggccacgc caggcgcata gaattttcgc cccatctccc gtcctgggcc ccttggcatg
     7921 tcgtctcccc tgatccgcat tcgcggtgcc cgccagcaca acctcaagaa cctcgacctc
     7981 gacattcgca ccggcgagtt gaccgtggtc accgggccca gcggctcggg caagtcgagc
     8041 ctggtgttcg acacgctgtt tgccgaaggc cagcgccgct acgtcgaaac cttcagcgcc
     8101 tacgcgcggc agtttctcga ccgcatggac aagcccgccg tggacaaggt cgaaggcgta
     8161 ccaccggcca tcgccattga ccagaccaat ccggtgcgct cgtcgcgctc caccgtcggc
     8221 acgatgacgg agctgaacga ccacctgaag ctgctgcttg cgcgcgcggg gcagttgttc
     8281 gatgcagaca ccgcgcttcc ggtgcggcac gacaacgccg acagcattta tgcggagatg
     8341 cagcggcgtg cgctggagtc ggacgatccg cgcctggtcg tgaccttcgc cgttgaactg
     8401 cccgccagcg cgacgacaga agaagttcag caatggctgt ccgccagcgg cttcacgcgc
     8461 gtgcaggccg agcgcgagag cgagggtaaa aagactctgg acgtggtggc cgaccgcttt
     8521 cgcatcggca gcgccgagcg tgcccgcgtg atcgaggcga tcgaggtcgc gctcaagcgc
     8581 ggcgctggcc gcatgatggt ctatgcgctc ccaaatcaag agctgcccgc gctggatgga
     8641 caagcgccag agggtgattt ggctttgaag tcggtgaccg cgccggtgct gtggaagttc
     8701 tccaccggcc tgcattgccc cgaaagcaac cggcgctaca gcgacccgat tccgtcgatg
     8761 ttctcgttca actcggcggt cggcgcgtgc gacacctgcc gcggctttgg gcgcgtgatc
     8821 ggcgtcgatt gggggctggt gattcccgac gagcgcctga cgctgcgcgc cggcgccatc
     8881 aagccgatcc agacgccggc gtggaaagaa gcccaggacg acttgatgcg ccacgccgaa
     8941 gccgccggca tcccgcgcga cacggcgtgg aacaagctca cgcctgaaca aaaagcctgg
     9001 gtgatcgacg gcacgcccaa ctacaaggaa ggcaactggg gcaagcagtg gtatggcatc
     9061 gcgcgcttct tcaagtacct ggagagcaag gcctacaaga tgcacatccg cgtgctgctg
     9121 tccaagtacc gcagctacac gccgtgcccc gactgcggcg gcgcgcgact caagacggag
     9181 ccgctgctgt ggcgcgtggg cacgaaggcc gatgccgatg cggtgctgga gccgtcgaag
     9241 cgcttcatgc ccgtcggggt ggcgtggtcg cgtgagcagc ttgaagcact gcctgggctg
     9301 tgcctgcacg acttgatgct gctgccgatc gatcggctgc gcatcttctt tgatcgactg
     9361 ggcgatcaga ttgctaccaa aacagaagct gctggcgctt atccatcaag cgctggcggc
     9421 gtttttgatg ccgattcggc ggacgccaag gcgctggcat tgatcctgga agaaatcacc
     9481 acccgcatca agtacctgtg cgatgtcggc atcggctacc tcacgctcga tcgtcaaagc
     9541 cgcacgctct ccggcggcga ggtgcagcgc atcaacctga ccaccgcgct cggcacgtcg
     9601 ctcgtcaaca cgctgttcgt gctcgacgag cccagcatcg gcctgcaccc gcgcgacatg
     9661 aaccgcatca ccgaagccat gcagcgcctg aaatccgccg gcaacacgct ggtggtggtc
     9721 gagcacgacc ccgccgtcat gctggcggcc gaccgcgtgc tcgacttcgg ccccggcccc
     9781 ggcgcggccg gcgggcgcat cgtgttcgat ggcacgccgg acgaactgcg cgccgccgac
     9841 acgctgaccg gcgcctacct gggcgggcgg cgccacatcg gtctgggcta cgcgcggccg
     9901 gttgcagacc acacgccgcg cctgatcctg gaaggcgcga ccgagcacaa cctgaagcag
     9961 gtcacggtgg atttcccgct gcagcggttg gtggtcatca cgggcgtcag cggctcgggc
    10021 aaatcgacgc tggtgcaaga cgtgctgtcg cccgcgctgc tgcgccactt cggcaaggcg
    10081 accgaatcgc ccggcgcaca cgagcgcttg ctgggcgccg atcatttgag cgacgtggtg
    10141 tttgtcgacc agtcgcccat cggcaagacg gcgcgctcca acccggccag ctacgttggc
    10201 gcgtgggacg cgatccgcgc gctgttcgcg caggcgccgc tggccaaaca gcgcggctac
    10261 acggcggcca agttcagctt caactcgggc gacggccgct gcgcgacctg cggcggctcg
    10321 ggcttcgagc acgtcgagat gcagttcctg tccgacatct acctgcgctg ccccgactgc
    10381 gacggcaagc gctatcggcc ggaaattctc gaagtcaccc tccagcgccc cgtaggtaaa
    10441 ttggggtcgg attccaattt ccaggacgct gctgatcgac tggctcaggc cgtcgatggg
    10501 aaactggacg ctgaccccca tttgccggtc caccgcaagc gactcttgaa cgtcgccgac
    10561 gtcctggacc tcaccgtcgc cgaagccgcc cacttgttcg cccaagaccg cgaggtgatc
    10621 cgcgcgctgc aacccatcgt cgacgtgggg ctcgattacg tcaagctggg ccagcccgta
    10681 ccgacgctca gcggcggcga agcgcagcgc ttgaagctgg ccggctttct ggccgacgcc
    10741 gcccaaagcg cgacggccag ccgacagccg gccgcgcgca agggcacgct gttcctgttc
    10801 gacgaaccga ccaccggcct gcacttcgac gacatcgcca agctgatgcg cgccttccgc
    10861 aagctgctgg acgcggggca ctcgctgatc gtcatcgagc acaacctgga cgtgatgcgc
    10921 gcggcggatt ggctgatcga cctcggcccg gaaggcggcg aagccggcgg ccaggtggtg
    10981 gccgaaggca cgccggacga ggtgatgcaa cacaccacat cgcacaccgg cgctgcgctg
    11041 cgggcctacg cgcaggccat cggcgccgcg gtgggtgtgt ctgccgaaga acccaggtcg
    11101 ctatacaaaa cagagctgct agcgctggat acacctgcac aggaggctca aaacagcatt
    11161 gaaatcgtgc acgcgcgcga gcacaacctc aagaacctga gcgtcgacat cccacgcggc
    11221 aagttcaacg tcatcaccgg cgtcagcggc tcgggcaaat ccacgctggc cttcgacatc
    11281 ctgttcaacg aaggccagcg gcgttatctc gaatcgctca acgcctacgc gcgcagcatc
    11341 gtgcagccgg cgggtcggcc cgaggtcgat gcggtgtacg gcatcccgcc gacggtggcc
    11401 atcgagcagc gcctgtcgcg cggcgggcgc aaatccaccg tgggcacgac gaccgaggtg
    11461 tggcactttc tacgcctgct ctacgtcaag ctgggcgtgc agcactgcgt gcacgacggc
    11521 gccgccgtgc agccgcagac gcccgagcgc attgccgcgc agatcatgac gcgctatcgc
    11581 ggccagacga ttggtctgct ggcgccgctg gtcgtcggcc gcaagggcgt gtacaccgag
    11641 ctggccgact gggcgcgccc gcgcggcttc acgcatctgc gggtcgatgg cgagttcttg
    11701 ccgaccacga acttcccgcg catcgaccgc ttcaaggaac acaccatcga gctgcccgtg
    11761 atgagcctgc ccgtcacgcc cggcaacgag gcgcagttgc gcgaatcgct cacgcgcgcg
    11821 ctggagcatg gcaagggcgt ggtgcatgtg ctgcacgatc tggccggact gcgcaacgcg
    11881 atggagacgg ggcaatcgac ggcacgcatc gggcaggtgg aggtgttctc gaccaagcgc
    11941 gcgtgccccg tgtgtgcgac gtcgtacgct gaactcgatc cgcgcctgtt ctcgtacaac
    12001 agcaagcacg gctggtgccc cgactgcgtc ggcacgggtg tcaagctcac gcgcgagcag
    12061 cgcaaggcgc tcgatgattc ggtgctggcc gacgaccaga aggggcgcga gcagacgttt
    12121 gatgagcccg aggtggagga cgtgggcgac gtggtgtgcc cgacgtgcca gggaacgcgt
    12181 ttgaaccccg tcgcacgcgc tgtgctgctg gaagccggcg cgatggacgg catcgccatt
    12241 accgatttgg cgcgcttatc ggtgcacgat ctgcggacgt ggttcgagga cctgaagctc
    12301 gaaggccgcg acgccgacat cgcccgcgac ctgatccccg agatcaagag ccgcctcgac
    12361 ttcctcgcac aagtcggcct gggctacctc acgctggatc gcggcgcgcc gacgctgagc
    12421 ggcggcgagg cgcagcgcat tcgcctggcg gctcaactgg gcagcaacct gcaaggcgtg
    12481 tgctacgtgc tcgatgagcc gaccatcggc ctgcacgcgc gcgacaacca gatattgctc
    12541 gacgcgctgc acacgctctc cagcaagggc aacacgctgg tggtggtgga gcatgacgaa
    12601 gacaccatcc gccgcgccga gcacctgatc gacatcggcc ccggcgcggg cgtgcgcggt
    12661 ggccggctgg tggccgaagg cacggcggct gatctggaag cggcggaaga ttcggccacc
    12721 ggccgctatc tgcgtgaagc gatgcggcac ccgatgcagg cgcgccgtgc tattgaatca
    12781 gaagctgctc gcgcagacgg gatgggcgct gggggcagaa atagtgcttc tgcgtccgaa
    12841 gccacaccac gatggctgca tctgctgggc gcgcaactgc acaacctcaa gcaggtcgat
    12901 gtggcggtgc cgctgcagcg cctggtcgcc gtcacgggcg tcagcggctc gggcaagtca
    12961 acgctggcgc gcgatgtgct gctggccaat gtggccgcgt gggtcggcca gcgcgccacc
    13021 aaggccgggc gcgacgccat ggacgcgggc aaggcgccgc cgctgattgg ctgcaccggt
    13081 ctgcgcgggt ttgaatcggt cgaccgcgta ctcgaagtcg accagacccc catcggcaag
    13141 acgccgcgct cctgccccgc cacctacatc ggcttttggg acaccgtgcg caagctgttt
    13201 gccgacacgc tggaagccaa ggcgcgcggc tatggcccag ggcgtttcag cttcaacacc
    13261 ggcgaaggcc gctgcccggc gtgcgaaggc cagggcgtgc gcaccatcgg catgagcttt
    13321 ctgcccgacg tgaaggtgcc gtgcgaggtc tgccacggcg cgcgcttcaa ccccgagacg
    13381 ctggctgtga catggcgcgg caaaagcatc ggagatgtgc tgcagatgga agtcgatgaa
    13441 gcggtggatt tctttgcgtc gatgcccgcc atcgcgcacc cactgcaact cctgaaggac
    13501 gtgggcctcg gctacctcac gctcggccag cccagtccaa cgctgagcgg tggtgaggcg
    13561 cagcgcatca agcttgtcac cgaacttagc aaggtgcggg acgacgtggg ccgccgcggc
    13621 cagaaggcgc cgcacacgct ttacgtgctg gacgaaccca cagtgggcct gcacatgcag
    13681 gacgtcgaaa agctgatcca cgtactgcac cgcctggtga acggcggcca cagcgtgctg
    13741 gtgatcgagc acgacctcga cctgatcgcc gaggccgact ggatcatcga cctgggcccc
    13801 gaaggcggcg atggcggagg ccagttggtg gccgccggaa cgcccgaaga tgtggtgcgc
    13861 gccggaacgc acaccggcgt ggcgctggcc agggtgttgc agcgcgccgg tggcgtgaca
    13921 gtcgcggtgg cgcgaatcaa gtagctgctg gccacgcctt ggcgcgcctt cgtcgccccg
    13981 gtgacccaac tgcgggcttt ccttgatgcg cgggccggtc aaatcgggtt gaatcgtcga
    14041 agaactttct tcaggagccc gccgcatgca tcgccgtacc ctcatcgccc tgtgcaccgc
    14101 cgtcacgctt gcggggcctg cactggcgca gacctttccc accaagccga tcaagctggt
    14161 catcgccttc cccgcaggcg ggccgaccga catcaccatg cgcgcgctgg ccgatggcgc
    14221 cagcaaggcg ctgggccagc ctgtggtggt cgagaacaag ccgggcgccg gcggcacgct
    14281 gccggcgcag gcgctgcaga ccgcgccggc cgacggctac acgctggcac agattccgct
    14341 cggcgtgttt cgcctgccct acaccaccaa gatcaactgg gatccggtga aggacatcaa
    14401 ctacgtgctc aacgtgacgg gctacgcgtt cggcgtcgtg gtgccgaccg attcaccgat
    14461 caagagctgg acccatttcg tcgcctgggc caaggccaac cccggcaagc tcacctacgg
    14521 ctccaccggc accatgacca gcccgcacct gacgatggag ctggtggcgc aaaaggccgg
    14581 cctgcagctg acgcatgtgc cctacaaggg cagcgccgat ttgatgcagg cggtgctggg
    14641 cggccacatc atggcggcca gcgattcgac cggcttcgcg ccgcaggtcg aggccggcaa
    14701 gctgcgtgtg ctcaacacct ggggcgacaa gcggctcgac aagtttcccg acgcgccgac
    14761 gctgaaggaa ctgggctacg acatcgtgca gaactcgcct ttcggcatcg gcgcgccgcg
    14821 cgacacgccg cccgccgtgg tcaagcgcct gcacgacgcc ttcaagacgg cgatggagca
    14881 gccggcctac cttgacgcac tcaagcgcta cgacatggtg ccgatctaca tgaactcggc
    14941 gcagtacaag aagtttgcgc aagacacctt cacgcgcgag aaggcactga tcgagaagct
    15001 ggggctggcc aaaggctctt gagcgcgtat caggtgcggt cggccagcag cgcgcgcgcc
    15061 acctcggcaa aaccggcgcc gcgctcgccg cgcgtgatgt agcgcggcag gtacttcagc
    15121 ttggattcaa agcggcggat gttggccacg cccacgcttt gcggcaggtg ctcgaacatc
    15181 acctggtcgt tggtcgaatc gccgacgtag acccagcgct cggcttcggc cggcagatcg
    15241 cgatccagca actcgcgcgc gatccagcac gcgccctgcc acttgtcgtg cgtgccgatc
    15301 cagccgttga tgtggatgct gctgacggtg gcgtgcaggc cttcggcctg catcacggcg
    15361 acggcctggg cgatctgcgc ttcgtcaaga cgactgaact cgctgtggtc aatggcgatg
    15421 tcggtttcgc gccccggcga atcctgcgcc agcgcgctgc ccggcacttc gcgcagcacg
    15481 cgggccgcgg cgcgctgcag ctgctcaaag ttgcgcgccc gcatggcggc gtcctgttgg
    15541 tatatttttg atagctgctc gcgctggtca ggcaagcgct ggcggccgat ttgaccaatg
    15601 tcgcctggaa tcaacgccgc cgcgccgttc tcggccacga tggcgcgcag gggccaggcc
    15661 agcgcaaacg gtacgctcca gccgacgggc cgaccggtga cggcgatcac cggcaagccg
    15721 gcgtcgcgca aatcggccag tgcctgcaac gcatcgggcg tgatcgcgcc ctcggtggtc
    15781 agcgtgtcgt cgatgtcggt caacaagccg acgaccttcg accacgcgcc tgccgggcgt
    15841 tcaaccagcg cgcgcagcgg gcgtctcctc ggggtcgtcg gtcaggtcct cgctgagcgg
    15901 ctgcgacggc acgcgctcga catcgtggtg caagaacgcg atcagaaact gaatgccgat
    15961 gatcagcggc aacgccgcca gcatgacggt gccgctggtc gccggttgcc ccgtcgtcag
    16021 gctgcgcagc caggcccagc cgccgaacac cacacccagc ccgcacagca ccacgccgag
    16081 caggctgtac aggctgccga tgttgaaatc gcgcagcagg tacaggtaca cgtagcgctt
    16141 gaagaagcgg ttgacgtgct tgacgatgaa ctccggcagc accttcgaga ccttcaggct
    16201 cgattcctcg tccgcataca ccgcatccat cggcacgtcg tgcaccaccg cgcgcatggt
    16261 gttgaggtgg tagagcatgt cggtttcgaa gaagtagcgc ttctcgagct tgtgcaacgg
    16321 catctcgcgc aacacggcgg tgtggatggc cgtgtagccg ttggtcggat ccatgatcga
    16381 ccagtaaccg gtgctgagct tgttgatgaa cgacagcgcc gcgttgccga acagccggat
    16441 cggcggcatg cccttgagcg attcgggccg atagaaccgg ttgcccttgg tgtaatcggc
    16501 gcgtccacgt tcaatcggac gtgtgaagtg cgggatcagc gccggatcca tctgcccatc
    16561 gccatcgacc ttgacgacga tgtccatgcc ttcttcaatc gccgcccgat agccgctgcc
    16621 gacggcgccg ccgacgccct ggttttcttc atggaatatc acccgcacgc gcgggtcggt
    16681 gcaatgctgc tgcacgaacg cgccgctgtc ctgcgggcac ttgtcgtcca ccacgtagat
    16741 gcgctgcacc tgcggc
//
LOCUS       DAOMVO010000019        16440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_707_length_16440_cov_16.991822, whole genome shotgun sequence.
ACCESSION   DAOMVO010000019 DAOMVO010000000
VERSION     DAOMVO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16440
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_16440_cov_16.991822"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PKV30_02055"
     CDS             <1..934
                     /locus_tag="PKV30_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRL29035.1"
                     /translation="GHEGAGIVVEVGEGVTSVQPGDHVIPLYTAECGQCLFCKSGKTN
                     LCTAVRATQGKGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKIDPQANPE
                     QVCLLGCGVTTGLGAVKNTAKVQPGDSVAVFGLGGIGLAVIQGAKLAKAGRIIAIDTN
                     PGKFDLARTFGATDCVNPKDFDKPIQQVIVDMTGWGVDHSFECIGNVNVMRAALECAH
                     RGWGQSVVIGVAGAGQEISTRPFQLVTGRKWLGTAFGGVKGRTELPGMVEDAMSGKIQ
                     LAPFVTHTMPLTGINEAFDLMHEGKSIRSVVHYA"
     gene            1097..1927
                     /gene="fghA"
                     /locus_tag="PKV30_02060"
     CDS             1097..1927
                     /gene="fghA"
                     /locus_tag="PKV30_02060"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HRL29036.1"
                     /translation="MNRIEHHASFGGRQEVWQHASRSTGTDMKLGVYLPPAALAGEKC
                     PVLYWLSGLTCTEQNFITKAGAQQFAAQHGLIVVAPDTSPRGEGVPNYDAYDLGQGAG
                     FYVNATEEPWAPHFRMEDYVVDELPALIEQHFPASAARGIFGHSMGGHGALTLALRHP
                     GKYRSVSAFSPIVAPSQVPWGQKALKAYLGDDLAAWQAHDAVALIASVTERLPLLVDQ
                     GDADEFLAEHLKPELLQAACDKAGHPLTLRMQPGYDHSYYFIASFMGDHVAHHAKALS
                     "
     gene            complement(1934..2323)
                     /locus_tag="PKV30_02065"
     CDS             complement(1934..2323)
                     /locus_tag="PKV30_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29037.1"
                     /translation="MKAIAAPLALLILSAGPGALAQEVPAAFVGKWQADWINRAGRPT
                     QAELDLPAARKGSWQTYGQSRTNLCVGKQGPVYVESATPEQVVLAIKMSETIAGCDDS
                     QLTLTAAPGGGAPSGKWRSGGEVTLKR"
     gene            complement(2502..>3215)
                     /locus_tag="PKV30_02070"
     CDS             complement(2502..>3215)
                     /locus_tag="PKV30_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29038.1"
                     /translation="ATGGAAYSGKWSAMPASAKGKVPVVLFLHGSSGLGLKAIGEWQQ
                     WLAAQGIASVAPDSFALPDHVTYKSPIDKASYERIHALRASEIAPALAAVQTAPWADT
                     SKLVLAGTSEGAVPVARYAGREFAARMLFAWSCEPNYFVTEPRNAFEADKPVLNVISS
                     VDPFFSPTNTWLGNPAARGSCADALKDHKQAVIALVPGAPHTLLTLPQVHAVTGGFLG
                     SLLKRWPLPVRSGGSLQNQ"
     assembly_gap    3216..3315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3316..3565)
                     /locus_tag="PKV30_02075"
     CDS             complement(<3316..3565)
                     /locus_tag="PKV30_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29039.1"
                     /translation="MIRFVRPAVFTLAPLALIAALLGCAATPPVPKAAPTGEIKGTAW
                     AHTPQSLAASVQNANVVLPAQATGGAAYSGKWSAMPASA"
     gene            complement(3713..4840)
                     /locus_tag="PKV30_02080"
     CDS             complement(3713..4840)
                     /locus_tag="PKV30_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HRL29040.1"
                     /translation="MTAKLFTPTRVGAIEVANRLVMAPLTRNRAPHAVPTPLMAQYYA
                     QRADPVTGAGLIVSEATAISPQGQGYADVPGLYGTEQLDGWKQVTKAVHAAGGKMVCQ
                     LWHVGRVSHHLLQPDNGDPVAPSAIRANTKTYLIDGEGQGGFALTSQPRALHRHEIPQ
                     IVHDYAAAARNAVKSAGFDGVEIHGANGYLLDQFLKTGANQRTDDYGGSIENRSRLML
                     EVTRAVVDAVGHDRVGIRLSPVTPANDIVDADPQPLFDHLLRQLATLGLAYVHVIEGA
                     TGGPREVEGRPFDYARAKKAYRNAGGRGAWMVNNGYDPLMAEDAVASGRADLVAFGKA
                     FISMPDLTERIRRGGPFQGVDKATLYGGGAEGFTDYPALAS"
     gene            4919..6133
                     /locus_tag="PKV30_02085"
     CDS             4919..6133
                     /locus_tag="PKV30_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRL29041.1"
                     /translation="MFEPSSVPLRRLVWIALALSLGAAVSLGITRFAYGLLLPVMRED
                     LDWGYTLAGAMNTANAGGYLLGALATPWLMRRFGAARVLLAGALLASGFMAASGFFTA
                     AGPLLLQRLLAGVASAFVFVAGGLLAARLGAQAPQHAGLLLGLYYGGTGWGISASALA
                     VPGLLRAAADVPHGWSWAWWGLALLCLLATLVLWWPASILQKSELLAHTGQAPAAHLI
                     HEPLPWRRMMPALAGYTLFGVGYIGYMTFVIALLREQGVAPAAITLFYALLGLAVVAS
                     ARIWAGLLNRAKGGGALALLNALLGVATILPALTRAWPLVLTSGLLFGAVFLSVVAST
                     TAFVRHNLPQAQWAAGISAFTVLFALGQIVGPTVVGWVADGPGGLTRGLVYSAAALWL
                     GAALAWRQKPLA"
     gene            6137..6634
                     /locus_tag="PKV30_02090"
     CDS             6137..6634
                     /locus_tag="PKV30_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRL29042.1"
                     /translation="MSHRCGLHGTRVVAAHRAQDAKFASAREYGRTGVLQGSTAQKLM
                     QTYAGKKASTLALGALTPVPSYAEGVRLLKAGQIDTLVADELLIPKDAAIALRPEQLT
                     VEPYALVMRKGDAAFVDAVDKALHKILGGAQGKQLAQDAGLKVDHLTRDAWQSPNKQP
                     APPVL"
     gene            complement(6659..7264)
                     /gene="gstA"
                     /locus_tag="PKV30_02095"
     CDS             complement(6659..7264)
                     /gene="gstA"
                     /locus_tag="PKV30_02095"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HRL29043.1"
                     /translation="MKLYYSAGACSLSPHIALEESGLPYEAISAPTKTKLLPDGSDYR
                     KVNPLGYVPYLINDQGEGLAEGPAIVQYIADQAPGKKLAPANGTWARYQLQSWLNFIG
                     TELHKGFAPLFNPAAPDEVKQAAKDKIRERLTWVNGELAGKTYLMGDDFTVADGYLFT
                     VTNWAKPLQIDLSPFANLVAYRERVGARPAVQRAMKAEGLI"
     gene            complement(7479..8258)
                     /locus_tag="PKV30_02100"
     CDS             complement(7479..8258)
                     /locus_tag="PKV30_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRL29044.1"
                     /translation="MHIPSMKELVTPEEWQLRCDLAACYRLVELYGMSDLVFTHISAK
                     LPASVTGGEEQFLINPYGLLFDEITASSLVKVDMACNKLHESPFPVNPAGFTIHSAVH
                     AARPDVACVLHTHTRAGVAVSAQQAGVLPISQQSTFVLASLAYHDYEGVAFRPDEGPR
                     LQKDLGTANFLVLRNHGLLTCGPSVADAFLAMYTFENACRIQIDALAGQRGMDGLTPV
                     PQAIVDGTAQAMRVQTGGLGGAFVWPTMLRQLEKQGAGYRT"
     gene            complement(8286..>8546)
                     /locus_tag="PKV30_02105"
     CDS             complement(8286..>8546)
                     /locus_tag="PKV30_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRL29045.1"
                     /translation="CRPHCGACCIAPSISTPIPGMPQGKPAGVRCVQLDDALRCRIFG
                     RPERPAVCRQLRPSLEMCGPRDDRGAHAALYLARLEALTAPG"
     assembly_gap    8547..8646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8784..8963
                     /locus_tag="PKV30_02110"
     CDS             8784..8963
                     /locus_tag="PKV30_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRL29046.1"
                     /translation="MLKWAIIFAVIALVAGFLGFGGVAAGAAGIAKILFVLFLVIAVV
                     MLVLALMGGKAIANR"
     gene            complement(9062..10375)
                     /locus_tag="PKV30_02115"
     CDS             complement(9062..10375)
                     /locus_tag="PKV30_02115"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRL29047.1"
                     /translation="MATRSPAPAAAAPSHALPSYLDPNHLGPWGIYLQQVDRVTPYLG
                     PLARWVETLKRPKRALIVDVPIELDNGTIAHFEGYRVQHNVSRGPGKGGVRFHQDVTL
                     SEVMALAAWMSVKNAAVNVPYGGAKGGIRVDPRKLSMGELERMTRRYTSEIGIIIGPS
                     KDIPAPDVNTNEKVMAWMMDTYSMNEGATATGVVTGKPINLGGSLGRREATGRGVFTV
                     GTEAAKHIGLKIEGARVAVQGFGNVGGIAGKLFAEAGALVVAVQDHTGGVANDKGLNV
                     TKLLEHVAKTGGVAGFKGGEPLKEGEFWSCKCDILIPAALEGQITKANAPKIKAKMVI
                     EGANGPTTPEADDILQDRGVLVVPDVIANAGGVTVSYFEWVQDFSSFFWSEDEINARL
                     IKIMKDAFDAVWQTAQQYKVSLRTATFIVACTRILTARDLRGLYP"
     gene            10466..10741
                     /locus_tag="PKV30_02120"
     CDS             10466..10741
                     /locus_tag="PKV30_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRL29048.1"
                     /translation="MYKILEFVQMQIVSVTEFRARAAEMVDLVQKGETVRILRHGKPV
                     AELNPVKQTPDEATVPAWKKPFEPLHIQRADGKTGAQLIIEERESGW"
     gene            10741..11172
                     /locus_tag="PKV30_02125"
     CDS             10741..11172
                     /locus_tag="PKV30_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL29049.1"
                     /translation="MRVLFDTSALYKRYDAEPGHDRVMAISDDADDIVVAPHCKTEIA
                     SALNRQRHDGELTLADYTRILAVVQRDFGGFTCQAFGPTVEMLAIAAMESARLRAMDA
                     LHIATAQHAQVDLFVTADRRQASVARAVGLTTELIGEKDAP"
     gene            11169..13160
                     /gene="mnmG"
                     /locus_tag="PKV30_02130"
     CDS             11169..13160
                     /gene="mnmG"
                     /locus_tag="PKV30_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRL29050.1"
                     /translation="MIYPETFDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIETLGA
                     MSCNPSIGGIGKGHLVKEVDALGGAMALATDEGGIQFRILNSSKGPAVRATRAQADRV
                     LYKAAIRRRIENQPNLWLFQQAVDDLMVEGERVVGAVTQVGIAFRGRTVVLTAGTFLD
                     GRIHVGLDNYQAGRAGDPPAVSLSARLKELKLPQGRLKTGTPPRIDGRSIDFAACEEQ
                     PGDGMPGGQNPHMPVFSFMGRADMHPRQLPCWITHTNARTHEIIRSGFDRSPMFTGKI
                     EGVGPRYCPSVEDKINRFADKESHQIFLEPEGLDTHEVYPNGISTSLPFDVQLALVRS
                     MKGLENAHILRPGYAIEYDYFDPRALKSSFETRDVKGLFFAGQINGTTGYEEAAAQGL
                     FAGINAALQCRQLDGRADDFGGSWLPRRDEAYLGVLVDDLITKGVTEPYRMFTSRAEY
                     RLQLREDNADMRLTEMGRALGLVDDARWDAFSRKRDAVSRETERLKSTWVNPRNLPAA
                     ESERVLGKAIEHEHNLFDLLRRPDVDYEALMSLDGGKHASPDVSRETLGELSAAVIEQ
                     VEIAAKYSGYIDRQRDEVERAAYYEGLRLPPELDYLQIPALSIEVRQKLQKHRPETLG
                     QAGRISGVTPAAISLLLIHLKKGGFKGFTGVAPAVAKAA"
     gene            13157..13810
                     /gene="rsmG"
                     /locus_tag="PKV30_02135"
     CDS             13157..13810
                     /gene="rsmG"
                     /locus_tag="PKV30_02135"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRL29051.1"
                     /translation="MSHPQQQRLAEGATALGLNLAAAQHAQLLAYLDLLAKWNKVYNL
                     TAVRDPAEMLTHHLLDSLAVIAPLVRHTQGGAARVLDVGSGGGLPGVVIAICCPEMSV
                     ACVDAVAKKAAFVQQAAGVLGLSNLRGVHARVESITEPYDVITSRAFATLADFTQWSA
                     GSLGEDGVWMAMKGKHPTDEIAALPPSVAVFHVEQLAVPGLDAERCLIWMRPAKPLP"
     gene            13807..14244
                     /locus_tag="PKV30_02140"
     CDS             13807..14244
                     /locus_tag="PKV30_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29052.1"
                     /translation="MSAERFTRSPALRTALWGGLSAGPMLALLWPVFVRPESGVWPAS
                     PLALTLAMLTLGTLTGLAVAFTMMLPVLSLLDLRPGPRLRRTAVAAGGGVCLVLVTLF
                     GLFSGGERLGSQWPLGLLLTIVGALCGWLGAMAVPAARPACPE"
     gene            14260..14874
                     /locus_tag="PKV30_02145"
     CDS             14260..14874
                     /locus_tag="PKV30_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRL29053.1"
                     /translation="MPAITDYPAFVIAILLFLAIPGPGNLAIISSTAQGRVRGGLAST
                     FGIIAGDQILMWCAVAGVAALLKAYPAAFAAVQWLGAAYLAYLGVRLLLAKPGQGAVL
                     TLKPGHFFKQSFFITLLNPKAIVFYMAFFPLFIDPTRQPGLATFGAMALTIAVLTFLY
                     GLVATLLAHHLAERVRASPRMSSALSKLAGMCLLGFGLKLAISK"
     gene            14906..15688
                     /locus_tag="PKV30_02150"
     CDS             14906..15688
                     /locus_tag="PKV30_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL29054.1"
                     /translation="MAHIFCIANQKGGVGKTTTTVNLAAGLAKIGQRVLMVDLDPQGN
                     ATMGSGVDKRAMALSVYDVLLESASIAEAASVPDKLKDAGCAYAVLGANRELAGAEVE
                     LVGEERRERRLKDALAAVDGDYDFVLIDCPPSLSMLTLNGLCAAQGVIVPMQCEYFAL
                     EGLADLTNTIKQVHANLNRDLRLIGLLRVMFDPRITLQQQVSEQLKTHYGPKVFDAVI
                     PRNVRLAEAPSYGLPGVVFDPSAKGSKAFVEFAEELVARGLR"
     gene            15802..>16440
                     /locus_tag="PKV30_02155"
     CDS             15802..>16440
                     /locus_tag="PKV30_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRL29055.1"
                     /translation="MSNLSAARAPASEPWIDWGKAIAAQCIVWHHLVHYGPLAPRLRL
                     WLPRLVDWLDQRALLAVQVFLVMGGYLAARSLWPQPGRPRITLADWPRRMAQRWRRLM
                     PVYLAALALALLGAALARHGLQDADTPAAPTLAQLAANALLLQDVLGHDALSAGLWYV
                     AIDLQLFALLAALAAVAALWQPAATPPPWRGPLRTRTRTIAAPARRAPALWGV"
BASE COUNT         2519 a         5296 c         5666 g         2759 t
ORIGIN      
        1 gggccacgaa ggcgccggca tcgtggtgga agtgggcgaa ggcgtgacca gcgtccagcc
       61 gggcgaccac gtcattccgc tgtacaccgc cgaatgtggc cagtgcctgt tctgcaagag
      121 cggcaagacc aacctgtgca cggccgtgcg cgcgacgcag ggcaagggcg tcatgcccga
      181 tggcaccacg cgcttttcgt acaacggcca gccgatctac cactacatgg gctgcagcac
      241 gttcagcgaa tacaccgtgg tggcggaagt gtcgctggcc aagatcgacc cccaagccaa
      301 ccctgagcag gtctgtttgc tcggctgcgg cgtgaccacc gggctgggcg cggtgaagaa
      361 caccgccaag gtgcagccgg gcgattcggt ggcggtgttt ggcctgggcg gcatcggcct
      421 ggcggtgatt cagggcgcaa agctggccaa ggccggccgc atcatcgcca tcgacacgaa
      481 tccgggcaag ttcgatctgg cgcgcacgtt tggcgccacc gattgcgtca accccaagga
      541 tttcgacaag ccaatccagc aggtgatcgt cgacatgacc ggctggggcg tggaccacag
      601 cttcgagtgc atcggcaacg tcaacgtgat gcgcgccgcg ctggaatgcg cgcaccgcgg
      661 ctggggccag agcgtggtca tcggtgtggc cggcgcgggg caggaaatct cgacgcgccc
      721 gttccagctg gtcaccggcc gcaagtggct gggcacggcc ttcggcggcg tcaaggggcg
      781 cacggagctg cccggcatgg tcgaagacgc catgagcggc aagatccagc tcgcgccctt
      841 cgtcacccac accatgccgc tcacgggcat caacgaggcc ttcgatctga tgcacgaggg
      901 caagtcgatc cgctcggtgg tgcactacgc ctgaagaatt gagcgaatgc ggcgctggcc
      961 ggcgcggaac cagcgccgca agctatcaaa tcaatagttt cgtggcctgg gttagcgcag
     1021 cgtcacccag cgaatttcgc cattccgcgc tgggttttgt gcctcaaccc agccgacttg
     1081 aatgcaggag cgcttcatga atcgcatcga acaccacgcc agcttcggcg gccggcagga
     1141 agtctggcag cacgccagcc gcagcaccgg caccgacatg aagctcggcg tctatctgcc
     1201 gcccgccgcg ctggcgggcg agaaatgccc ggtgctgtac tggctgtcgg gcctgacctg
     1261 caccgagcag aacttcatca ccaaggcggg cgcccagcaa ttcgcggcgc agcacggcct
     1321 gatcgtggtc gcgcccgaca ccagcccgcg cggtgaaggc gtgcccaact acgatgccta
     1381 cgatttgggc caaggcgcgg gcttttacgt gaacgcgact gaagagccct gggcgccgca
     1441 cttccgcatg gaagactacg tggtggatga actgccggcg ctgatcgagc agcactttcc
     1501 ggccagcgcg gcgcgcggca tcttcggcca cagcatgggt ggccacggcg cgctgacgct
     1561 ggcgctgcgc catcccggca agtaccggag cgtgagcgcg ttctcgccca tcgtcgcgcc
     1621 ctcgcaggtg ccctgggggc agaaggcgct caaggcgtat ctgggcgacg acctggccgc
     1681 atggcaggcg cacgacgccg tggcgctgat cgcatcggtc accgagcgcc tgccgctgct
     1741 ggtggaccag ggcgatgccg acgagtttct ggccgagcat ctgaagcccg aactgctgca
     1801 ggccgcctgc gacaaggccg gtcacccgct gacgctgcgg atgcagccgg gctacgacca
     1861 cagctactac ttcatcgcca gcttcatggg cgatcacgtg gcgcaccacg cgaaggcctt
     1921 gagctgaggc ggtttagcgc ttcagcgtga cctcgccacc gctgcgccac ttgccgctgg
     1981 gcgcgccgcc gccgggcgcg gcggtcagcg tcaattgcga gtcatcgcag ccggcaattg
     2041 tctcggacat cttgatcgcc agtaccactt gctctggcgt cgcgctctcg acatagaccg
     2101 gcccctgctt gccaacgcac aggttggtgc gcgactggcc gtaggtctgc caactgccct
     2161 tgcgcgcggc tggcagatca agctcggcct gggtcggcct gccggcccgg ttgatccagt
     2221 cggcctgcca tttgccgacg aaggcagcgg gcacttcctg cgccagcgcg ccggggccgg
     2281 ctgacaaaat cagcagcgcg aggggggcgg caatcgcttt catgggtttg ctccgggcgt
     2341 ccgcggtgtg cggacaactg cattgcagca gcgctggcgc gccgcgccca ttccccgaaa
     2401 gtaggaggcc gcctcaacct tgattcgagt cgaagtcgcg ctgcgataga taaaaatcca
     2461 accaaaacgg ccgccagcgc ttttctggat tgcgcagaca gctattgatt ttgaagcgat
     2521 ccaccagagc ggacaggcag cggccagcgc ttcagcaggc tgcccagaaa cccgcccgtc
     2581 accgcgtgca cttgtggcag cgtgagcagc gtgtgcggcg cgccggggac cagtgcgatc
     2641 acggcctgct tgtggtcctt cagcgcatcc gcacatgagc cgcgtgccgc agggttaccg
     2701 agccaggtgt tggtgggtga gaaaaatgga tcgacgctgc tgatgacgtt cagcaccggc
     2761 ttgtcggcct caaaggcgtt gcgcggctcg gtgacgaaat agttcggctc gcagctccag
     2821 gcaaacagca tccgcgcggc gaattctctg cccgcgtagc gcgccaccgg caccgcgcct
     2881 tcgctggtgc cggccagcac cagcttcgac gtgtcggccc acggtgcggt ctggaccgcc
     2941 gccagcgcgg gcgcgatctc gctggcgcgc aaggcgtgga tgcgctcgta gctggccttg
     3001 tcgatgggcg acttgtaggt cacgtggtcg ggcagcgcaa acgaatcggg cgccacgctg
     3061 gcgatgcctt gcgcggccag ccactgctgc cattcgccaa tcgccttcag ccccaggccg
     3121 gacgagccgt gcaggaacag caccaccggc accttgccct tggcggacgc gggcatcgcg
     3181 ctccatttgc ccgagtaggc cgcgccgccc gtggcnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnntggcg gacgcgggca tcgcgctcca tttgcccgag taggccgcgc
     3361 cgcccgtggc ctgcgccggc agcaccacgt tggcgttctg cacgctggcg gccagcgact
     3421 gcggcgtgtg cgcccaggcc gtgcctttga tttcgccggt cggggccgct ttcggcacag
     3481 gcggcgtggc ggcgcagccc agcagggcgg cgatcagcgc caagggggcc agggtgaaca
     3541 cggcggggcg gacgaagcgg atcatgaaca agccttcatt gaataagcca gcaccgatga
     3601 aaccgcagcg cgcgaggtgc caccagtcgg agatttcctg agcccgtcga tcggagtcaa
     3661 aagtgccgac agcgcttgtt ggacaagcgc aagcagctat caaaacaata gttcaactgg
     3721 ccagcgccgg atagtcggta aagccctcag cgccgccgcc gtacagcgtc gccttgtcca
     3781 cgccctgaaa cggcccgccg cgccggatgc gttcggtgag gtcgggcatg ctgatgaagg
     3841 ccttgccaaa ggcgaccaga tcggcgcggc cggatgcgac cgcgtcttcg gccatcagcg
     3901 ggtcgtagcc gttgttgacc atccaggcgc cgcgcccgcc agcgttgcgg tacgccttct
     3961 tggcgcgggc gtagtcgaag gggcggcctt ccacctcgcg cgggccgccg gtggcgcctt
     4021 cgatgacgtg cacgtaggcc aggcccagcg tggccagctg gcgcagcagg tgatcgaaca
     4081 gcggctgcgg atcggcgtcg acgatgtcgt tggccggcgt cacgggcgag aggcgaatgc
     4141 ccacgcggtc gtggcccacg gcgtcgacca cggcgcgcgt gacttccagc atcaggcgcg
     4201 aacggttctc gatcgagccg ccgtagtcgt cggtgcgctg gttggcgccg gttttcagga
     4261 actgatcgag caggtagccg ttggcgccgt ggatttccac gccgtcaaag ccggccgact
     4321 tgacggcgtt gcgcgcggcg gcggcgtagt cgtgcacgat ttgcgggatc tcgtggcggt
     4381 gcagcgcgcg cggctggctg gtcagcgcaa agccgccctg cccttcgccg tcgatcaggt
     4441 aagtcttggt gttggcgcgg atggccgagg gcgccaccgg gtcgccgttg tcgggctgca
     4501 gcaggtggtg cgacacgcgg cccacgtgcc acagctggca caccatcttg ccgcctgccg
     4561 cgtgcacggc cttggtgacc tgcttccagc catcgagctg ctcggtgccg tacaggccgg
     4621 gcacgtcggc atagccttgg ccttgcgggc tgatggcggt ggcttcgctc acgatcaggc
     4681 cggcgccggt gacgggatcg gcgcgctgcg cgtagtactg ggccatcagc ggcgtcggga
     4741 ccgcatgcgg cgcgcggttg cgcgtgagcg gcgccatgac gaggcggttg gcgacctcga
     4801 tggcgccgac gcgggtgggg gtgaagagct tggcggtcat ggcgatggcc tgtgcaggcg
     4861 tggttttggg gcattgtgct tgatcgcgcg ccgcatcggc ggcggtcaca atggccgcat
     4921 gttcgagccc tcttcagtgc cgctgcgccg tcttgtctgg atcgccctgg cgctgtcgct
     4981 cggcgccgcc gtgtcgctgg gcatcacccg tttcgcctac ggcctgctgc tgccggtgat
     5041 gcgcgaggac ctcgactggg gctacacgct cgccggggcg atgaacaccg ccaacgccgg
     5101 cggctacctg ctgggcgcgc tggccacgcc gtggctgatg cgccgctttg gcgccgcgcg
     5161 ggtgctgctg gcgggggcgc tgctggccag cgggttcatg gcggcctcgg gttttttcac
     5221 cgccgccggg ccactgttgc tgcagcggct gctggcgggg gtggccagcg ccttcgtgtt
     5281 cgttgcgggc gggttgctgg cggcgcggct gggcgcgcag gcgccgcagc acgcggggct
     5341 gctgctgggg ctgtattacg gcggcacggg ctggggcatc agcgcgtcgg cgctggcagt
     5401 gccggggctg ctgcgggcgg cggccgatgt gccgcacggc tggtcgtggg cctggtgggg
     5461 gctggcgctg ctgtgcctgc tggccacgct ggtgctgtgg tggccggcat cgatactaca
     5521 aaaatcagag ctgcttgcgc acactgggca agcgccagcg gcccatttga ttcatgaacc
     5581 gctgccctgg cgccgcatga tgccggcgct ggcgggctac acgctgtttg gcgtcggcta
     5641 catcggctac atgacgttcg tgatcgcgct gctgcgcgag cagggcgtgg cgccggcggc
     5701 gatcacgctg ttttacgcgc tgctgggcct ggcggtggtg gcgtcggcgc gcatctgggc
     5761 cgggctgctg aaccgcgcca agggcggcgg cgcgctggcg ctgttgaacg cgctgctggg
     5821 cgtggcgacg atcttgccgg cgctgacgcg ggcgtggccg ctggtactga cctcggggct
     5881 gctgtttggc gcggtgtttc tgtcggtggt ggcatcgacc accgcctttg tgcgccacaa
     5941 cctgccgcag gcgcagtggg cggcgggcat cagcgccttc acggtgctgt ttgcgctggg
     6001 gcagatcgtc gggcccacgg tggtcggctg ggtggccgat ggtccgggcg ggttgacgcg
     6061 cgggctggtc tattcggccg ccgccctgtg gctgggcgcg gcgctggcct ggcggcagaa
     6121 gccgctggcg tgagctttgt cgcaccggtg cggcctgcac ggcacgcgcg tggtggccgc
     6181 gcaccgcgcg caagacgcca agttcgcctc ggcgcgcgaa tacggccgca ccggcgtgct
     6241 gcagggctcg acggcgcaaa agctgatgca gacctacgcc ggcaagaagg ccagcacgct
     6301 ggcgctgggc gcgttgacgc cggtgcccag ctatgccgaa ggcgtgcgcc tgctcaaggc
     6361 cggccagatc gacaccctgg tggccgatga attgctgatt ccgaaagacg ccgccatcgc
     6421 gctgcggccc gagcagctga cggtggagcc ctatgcgctg gtcatgcgca agggcgatgc
     6481 ggcgtttgtc gacgcggtgg acaaggcgct gcacaagatt ctgggcggcg cacagggcaa
     6541 gcaactggcg caggacgccg gcctcaaggt cgaccacctg acgcgcgacg cctggcaaag
     6601 ccccaacaag cagccggcgc cgccggtgct ttgaggggtc aggggcgcgg cgcgcgcctc
     6661 agatcagccc ttccgccttc atcgcccgct gcacggcggg gcgggcgccg acacgttcgc
     6721 ggtacgcgac caggttggcg aagggtgaca ggtcgatttg cagcggcttg gcccagttgg
     6781 taacggtgaa caggtagccg tcggcgacgg tgaagtcgtc gcccatcaaa taggtcttgc
     6841 cggccagttc gccgttgacc caggtcaggc gctcgcgaat cttgtccttg gcggcttgct
     6901 tgacttcgtc tggcgcggcg gggttgaaca gcggggcaaa gcccttgtgc aactcggtgc
     6961 cgatgaagtt gagccagctt tgcagctggt agcgcgccca ggtgccgttg gccggcgcca
     7021 gcttcttgcc cggcgcctgg tcggcgatgt actgcacgat ggcggggcct tcggccaggc
     7081 cttcgccctg gtcgttgatg aggtagggca cgtagcccag cgggttgacc ttgcggtagt
     7141 cggagccgtc gggcagcagc ttggtcttgg tgggggccga gatggcttcg tagggcaggc
     7201 cggattcttc cagcgcgatg tggggcgaca gcgagcaggc gccggcagag tagtagagct
     7261 tcatggtgtt gtccttcttc gttgatgaaa cagttgataa gtcaaatcgg ccttcagagc
     7321 agatggagta agcgcgagca gctcacaaaa acagagcaaa tggcgtgcgg gcacctggaa
     7381 ggccgatcgc ccgcgcgctg cacgcgacgg agcgaaggat cagcgtagca aaagccgcga
     7441 cggcgtgcag gcggcggggc gccaatcctg cgtgccgatc aggtgcgata gccggcgccc
     7501 tgcttctcga gctggcgcag catggtcggc cacacgaagg cgccgcccag gccgccggtc
     7561 tgcacgcgca tggcttgcgc ggtgccgtcg acgatggcct gcggcacggg cgtcaggccg
     7621 tccatgccgc gctggccggc cagcgcgtcg atctggatgc ggcaggcgtt ctcgaacgtg
     7681 tacatggcca gaaaggcatc ggccacactg gggccgcagg tcagcaggcc gtggttgcgc
     7741 agcaccagaa agttggccgt gcccaggtct ttttgcaggc gcgggccttc gtcggggcgg
     7801 aaggccacgc cttcgtagtc gtggtacgcc agcgaggcca gcacgaaggt gctttgctgg
     7861 ctgatgggca gtacgcccgc ctgctgcgcg ctgacggcga cgccggcgcg cgtgtgggtg
     7921 tgcagcacgc aggccacgtc gggccgcgcc gcgtgcaccg cgctgtggat ggtgaagccg
     7981 gccgggttga cggggaaggg cgactcgtgc agcttgttgc acgccatgtc gaccttgacc
     8041 aggctggagg cggtgatttc gtcaaacagc aggccgtagg ggttgatgag gaactgctct
     8101 tcgccgcccg tgacgctggc cggcagcttg gcgctgatgt gggtgaacac gaggtcgctc
     8161 atgccataca actcgacgag gcggtagcag gccgccaggt cgcagcgcag ttgccactct
     8221 tcgggcgtga ccagttcttt catcgacggg atgtgcatgg cgggtctcct ttgtatgggc
     8281 gcagcttagc cgggcgcggt gagagcctcg agtcgcgcaa ggtacagcgc ggcgtgcgcg
     8341 ccgcggtcat cgcgcggacc acacatctca agcgacggcc gcaactggcg gcacacggcc
     8401 gggcgttcgg ggcggccgaa gatgcggcag cgcagggcgt cgtcgagctg cacgcagcgc
     8461 acgccggccg gcttgccttg gggcatgccg ggaatcggcg tgctgatgga aggcgcgatg
     8521 cagcaggcgc cgcaatgggg gcggcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnggtt tcggcttttc ctacgccgcg cgccgcgctc ggacgacagc cggcgcgccg
     8701 cggcggcgag aagatgagca cactgcttcc tcgcaaccgg ggcaggcgac acacagcaag
     8761 gttgttttgc aaaggagagc gtcatgttga agtgggccat tatttttgcc gtgatcgcgc
     8821 tggtcgcggg cttcctgggc tttggtggcg tggcggccgg tgctgccggt atcgccaaga
     8881 tcctgttcgt gctgttcctg gtcatcgccg tggtgatgct ggtgctggcg ttgatgggtg
     8941 gcaaggcgat agccaaccgc taagcgagcc ggtgcgcggt gttgatgccg cgttgacttg
     9001 gccgagaaaa aaagccgcgc ggccttgagg ctgcgcggct ttttcttttg cgttcgctcg
     9061 ttcagggata cagaccgcgc agatcgcgcg ccgtcaggat gcgcgtgcag gccacgatga
     9121 aggtcgccgt gcgcaggctg accttgtact gttgcgcggt ttgccagacg gcgtcgaagg
     9181 cgtctttcat gatcttgatc aggcgggcgt tgatctcgtc ctcgctccag aagaagctgg
     9241 aaaaatcctg cacccactcg aagtagctca ccgtcacgcc gcccgcgttg gcaatcacgt
     9301 cgggcaccac cagcacgccg cggtcttgca ggatgtcgtc ggcctcgggc gtggtcgggc
     9361 cgttggcgcc ttcgatcacc atcttggcct tgatcttggg ggcgttggcc ttggtgatct
     9421 gcccttccag cgcggcggga atcaggatgt cgcacttgca cgaccagaat tcgccttcct
     9481 tcagcggctc gccgcccttg aagccggcca cgccgcccgt cttggcgacg tgttcaagca
     9541 gcttggtgac gttgagcccc ttgtcgttgg ccacgccgcc ggtgtggtcc tgcaccgcga
     9601 ccaccagcgc gcccgcttcg gcaaacagct tgccggcgat gccgcccacg ttgccgaaac
     9661 cctgcaccgc cacgcgcgcg ccttcgatct tgaggccaat gtgcttggcc gcctcggtgc
     9721 cgacggtgaa cacgccgcgc ccggtggctt cgcgccgacc gagtgagccg cccaggttga
     9781 tcggcttgcc cgtgaccacg ccggtggccg tggcgccttc gttcatcgag taggtgtcca
     9841 tcatccacgc catcaccttc tcgttggtgt tgacgtcggg cgcggggatg tccttgctcg
     9901 ggccgatgat gatgccgatc tcggacgtgt agcggcgcgt catgcgctcc agttcgccca
     9961 tcgacagctt gcgcggatcg acccggatgc cgcccttggc gccgccgtag ggcacgttga
    10021 cggccgcgtt cttgaccgac atccacgccg ccagcgccat cacttccgac agcgtgacgt
    10081 cctggtgaaa gcgcacgccg cccttgcccg ggccacggct gacgttgtgc tgcacgcggt
    10141 agccctcgaa gtgggcgatg gtgccgttgt ccagctcgat cggcacatcg acgatcagcg
    10201 cgcgcttggg gcgcttgagc gtctcgaccc agcgcgcgag cgggcccagg tagggcgtga
    10261 cgcggtcgac ctgctgcagg tagatgcccc aggggccgag gtggttgggg tccaggtagg
    10321 acggcagcgc gtggctgggc gctgcggcgg caggggcggg tgaacgggtg gccatttcag
    10381 tctcctcgat tgttgtgggt gccagggtga cagcatacgc gaggcgtgcg acaccaccca
    10441 gcaggctttc cgatgatcgg ctaaaatgta caaaatactg gagtttgtac aaatgcagat
    10501 cgtgtccgtg accgaattcc gcgcccgcgc tgccgaaatg gtcgaccttg tgcagaaggg
    10561 cgaaaccgtg cgcatcctgc ggcatggcaa gccggtggcg gagctgaatc ccgtcaagca
    10621 gacgccggac gaagcaacgg tgccggcttg gaaaaagccg ttcgagccgc tgcatattca
    10681 gcgggccgat ggaaaaacgg gtgcgcaact catcattgaa gaacgggaga gcggctggtg
    10741 atgcgcgtgc tgttcgatac ctcggcgctc tacaagcgct acgacgccga acctggccat
    10801 gaccgcgtga tggccatcag cgatgacgca gacgacatcg tggtggcgcc acactgcaag
    10861 accgaaattg catcggccct gaaccggcaa cgccacgatg gcgaactgac cctggccgac
    10921 tacacgcgca tcctggccgt ggtgcagcgc gattttggcg gattcacctg ccaagccttt
    10981 gggccgaccg tcgagatgct ggcgattgct gccatggaat cagcgcgctt gcgtgccatg
    11041 gacgcgctgc acattgccac ggcccagcac gcgcaagtcg atttgttcgt gacggccgac
    11101 cgacgccagg cctccgtggc gcgcgcagtc ggcttgacga cggaactgat tggggaaaag
    11161 gacgcgccat gatctatccc gaaaccttcg acgtcatcgt cgtcggcggc ggccacgccg
    11221 gcaccgaggc ggcgctggcg gccgcgcgca tgggcgcgca gacgctgctg ctcacccaca
    11281 acatcgagac gctgggcgcc atgagctgca accccagcat tggcggcatc ggcaagggcc
    11341 atctggtgaa agaggtggat gcgctgggcg gcgccatggc gctggccacc gacgagggcg
    11401 gcatccagtt ccgcatcctc aactcaagca aaggcccggc cgtgcgcgcc acgcgggcgc
    11461 aggccgatcg cgtgctgtac aaggccgcga tccgtcgacg catcgagaac cagccgaacc
    11521 tgtggctgtt ccagcaggcg gtggacgacc tgatggtcga gggcgagcgc gtggtgggcg
    11581 ccgtcacgca ggtcggcatc gctttccgcg gtcgcaccgt ggtgctgacg gcgggcacgt
    11641 ttctggacgg gcgcatccac gtggggctgg acaactacca ggcggggcgc gcgggcgatc
    11701 cgcccgcggt gtctttgtcg gcacgcctga aagagttgaa gttgccgcaa ggccgcctga
    11761 aaaccggcac gccgccgcgc atcgacgggc gcagcatcga ctttgccgcc tgcgaagagc
    11821 agcctggcga cggcatgccc ggcggccaga acccgcacat gcccgtgttc agcttcatgg
    11881 gccgcgccga catgcacccg cgccagttgc cgtgctggat cacgcacacc aacgcgcgca
    11941 cgcacgagat cattcggtcg gggttcgatc gcagccccat gttcaccggc aagatcgagg
    12001 gcgtcggccc gcgctattgc cccagcgtgg aagacaagat caatcgcttt gcggataaag
    12061 aaagccacca gatcttcttg gagccggaag ggctggacac gcacgaggtg taccccaacg
    12121 gcatcagcac cagcctgccg ttcgacgtgc aactggcact ggtgcgctcg atgaaggggc
    12181 tggagaacgc ccacatcctg cggcccggct atgccattga atacgactac ttcgacccgc
    12241 gcgcgctcaa aagcagtttc gaaacgcgcg acgtgaaggg cctgttcttt gccggccaga
    12301 tcaacggcac tacaggttac gaggaggcgg cggcgcaagg cctgttcgcc ggcatcaacg
    12361 ccgcgctgca atgccgccag ctcgacggcc gcgccgacga ctttggtggc tcatggctgc
    12421 cgcgccgcga cgaggcttac ttgggcgttc tggtggacga cctcatcacc aagggcgtga
    12481 ccgagcccta ccgcatgttc accagccgcg ccgaataccg actgcaactg cgcgaagaca
    12541 acgcggacat gcgcctgacc gaaatgggcc gggcgctggg cctggtggat gacgcgcgct
    12601 gggatgcgtt cagccgcaaa cgcgatgctg tttcacgtga aacagagcgg ctgaaatcca
    12661 cctgggtgaa tccgcgcaac ttgccagcgg cggaatccga gcgcgtgctg ggcaaggcca
    12721 tcgagcatga gcacaacctg ttcgatttgc tgcgtcggcc ggacgtggac tacgaagcac
    12781 tcatgagcct ggatggcggc aagcacgcca gccccgacgt ttcacgtgaa acactgggtg
    12841 aactgtccgc tgccgtcatc gagcaggtcg agatcgccgc caagtattcc ggctacatcg
    12901 accgccaacg tgacgaggtg gagcgcgccg cgtactacga ggggctgcgc ctgccgcctg
    12961 aactcgacta tctgcagatc cccgcgctgt ccatcgaagt gcgtcagaag ctgcaaaagc
    13021 accggcccga gacgctgggg caggccggcc gcatcagcgg cgtgacgccc gctgccatct
    13081 ccttgctgct gatccatttg aagaagggtg gcttcaaggg cttcaccggc gtcgcacccg
    13141 ccgtcgccaa ggcggcatga gccacccgca acaacagcgc ctggccgaag gcgccactgc
    13201 gctcggactg aatctcgctg ccgcgcagca cgcgcaactg ctggcgtatc tcgatttgct
    13261 cgccaagtgg aacaaggtct acaacctgac cgccgtgcgc gacccggccg agatgctgac
    13321 gcaccacctg ctcgacagcc tggccgtcat cgcgccgctg gttcgtcaca cgcagggcgg
    13381 ggcggcgcgc gtgctggatg tgggctcggg cggtggtttg cccggcgtcg tcatcgccat
    13441 ctgctgcccg gaaatgtccg ttgcctgtgt cgatgccgtt gccaagaagg cggcgtttgt
    13501 ccaacaggcg gccggcgtgc tcggcctgtc gaacctgcgc ggcgtgcatg cccgggttga
    13561 atcgatcacc gagccctacg acgtcatcac ctcccgcgcc ttcgccacgc tggccgactt
    13621 cacccaatgg tcggccggct cgttgggcga ggacggtgtg tggatggcga tgaagggcaa
    13681 gcatcccacc gacgaaatcg ccgcactgcc gccatccgtc gccgtgtttc acgtggaaca
    13741 actcgccgtg ccgggtctgg acgccgagcg ctgcctgatc tggatgcgcc cggccaagcc
    13801 tttgccatga gtgccgagcg cttcacccgc tcgccggctt tgcgcaccgc gctctggggt
    13861 gggttgagcg ccgggccgat gctggcgctg ctgtggccgg tgttcgtgcg gccggagtcc
    13921 ggcgtctggc ccgcttcgcc actggccctg accctggcca tgctgacgct cggcacgctc
    13981 acgggcctgg ccgtcgcctt caccatgatg ctgccggtgc tgagcctgct cgatctgcgc
    14041 cccggcccgc gcctgcgccg gacggctgtg gcggccgggg gcggcgtgtg tctcgtcctg
    14101 gtcacgcttt tcggtctttt ctcgggtggc gaacgccttg gatcgcagtg gcccttgggc
    14161 ttgcttctta caatcgtcgg ggcgctgtgt ggctggctgg gtgcgatggc cgtcccagcc
    14221 gctcgcccgg cctgcccgga ataaacagct cgcccgccca tgcccgccat caccgactac
    14281 cccgcctttg tcattgccat cctgctgttc ctggccattc ccggcccggg caacctggcc
    14341 atcatttcca gcacggcgca agggcgggtg cgcggcgggc tggcgtcgac cttcggcatc
    14401 atcgcgggcg atcagattct gatgtggtgc gcggtggccg gcgtggcggc cttgctcaaa
    14461 gcttatccag cggcgttcgc ggcggtgcag tggctgggcg cggcgtacct ggcgtatctg
    14521 ggcgtgcgcc tgctgctggc caagccggga cagggcgcgg tgctgacgct caagccgggc
    14581 cactttttca agcaatcgtt cttcatcacg ctgctcaacc ccaaggccat cgtgttctac
    14641 atggccttct tcccgctgtt catcgatccc acccggcagc ccggcttggc cacgttcggc
    14701 gccatggcgc tcaccatcgc ggtgctgacc tttctgtacg gcctggtcgc cacgctgctg
    14761 gcgcaccatc tggccgagcg cgtgcgcgcc agcccccgca tgtccagcgc gctcagcaag
    14821 ctggcgggca tgtgtctgct gggctttggg ctcaagctgg cgatcagcaa gtaaaccgat
    14881 tcacgtcagc acccactctc acgccatggc gcatattttc tgcattgcca accagaaggg
    14941 cggcgtcggc aagacgacga ccacagtcaa cctggccgcc ggcctggcca agatcggcca
    15001 gcgcgtgctg atggtcgacc tcgacccgca gggcaacgcc accatgggct cgggcgtcga
    15061 caagcgggcg atggcgctgt cggtctacga cgtgctgctg gaatcggcca gcatcgccga
    15121 agccgccagc gtgcccgaca agctgaaaga cgccggctgc gcctacgccg tgctgggcgc
    15181 caaccgcgag ctggccggtg ccgaggtcga actggtcggt gaagagcgcc gcgagcgccg
    15241 cctgaaggac gcgctggccg ccgtcgatgg cgactacgac ttcgtgctga tcgactgccc
    15301 gccatctttg agcatgctca ccctcaacgg cctgtgcgcc gcgcagggcg tgatcgtgcc
    15361 catgcagtgc gaatacttcg cgctcgaagg cctggccgat ctcaccaaca ccatcaagca
    15421 ggtgcacgcc aacctgaacc gcgacctgcg cctgatcggc ctgctgcgcg tgatgttcga
    15481 cccgcgcatc acgctgcagc agcaggtcag cgagcagctc aagacgcatt acggccccaa
    15541 ggtgttcgac gccgtgatcc cgcgcaacgt gcgcctggcc gaggcgccca gctacggcct
    15601 gcccggcgtg gtgttcgacc cgtcggccaa gggcagcaag gcgtttgtcg agttcgcaga
    15661 ggaactggtg gcacgcggct tgcgctgagc ggggcggctg tgcggcgatg aggcgccgca
    15721 cattccgaaa cgtgccgcct gtcatttcag gttaaaaacg gccctggcgc ttgtccagaa
    15781 agcgcgagca gctatcaaat catgagtaat ctttcagccg cccgggcgcc cgccagcgaa
    15841 ccctggatcg actggggcaa ggccatcgcc gcccagtgca tcgtgtggca ccacctggtg
    15901 cactacggcc cgctggcgcc gcgcctgcgg ctgtggctgc cacggctggt cgattggctc
    15961 gaccagcgtg cgctgctggc ggtgcaggtg tttctggtga tgggcggcta cctggccgca
    16021 cgcagcctgt ggccgcaacc ggggcgcccg cgcatcacgc tggccgactg gccgcgccgc
    16081 atggcgcagc gctggcgccg gctgatgccg gtctacctgg cggcgctggc actggcgctg
    16141 ctgggcgccg cgctggcgcg ccatggcctg caggacgccg acacccccgc cgcgcccacg
    16201 ctggcacaac tggcggccaa cgcgctgctg ctgcaagacg tgctgggtca cgacgccctg
    16261 agcgccggtc tgtggtacgt cgccatcgac ctgcaactgt ttgcgctgct ggctgcgctg
    16321 gccgccgtgg cggcgctgtg gcagccggcc gccacgccgc cgccctggcg cgggcccttg
    16381 cgcacgcgga cgcgcacgat cgcggcgccc gcacgtcgtg cgccggcgct gtggggcgtc
//
LOCUS       DAOMVO010000020        16179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_724_length_16179_cov_15.149963, whole genome shotgun sequence.
ACCESSION   DAOMVO010000020 DAOMVO010000000
VERSION     DAOMVO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_724_length_16179_cov_15.149963"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(586..1497)
                     /locus_tag="PKV30_02160"
     CDS             complement(586..1497)
                     /locus_tag="PKV30_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29056.1"
                     /translation="MKGEALFKCGDCSQIGPAKKKLHGAWYLLLPVWFVGAIFAVALP
                     YVGIPALMILYFLMRKNHCTSCGSMNVERYFTEEERQKMNEAKEEMARKKQEDAVMRE
                     IKQSQKASENQKIINLKMDLNLLPDGAKKLRVKVVGGAGWEPMHDTSIDVKIASDALV
                     FYDFKRIKKISLPYGEIRSIDIGGPGKVTSGGGFAGGGFGEGALVGMGIATLANILTT
                     HSSTKTYVRLLLEDAELILLSSDTEPDKMRILLSPLFVKSTKMLEHKPEIDSNSMTRQ
                     LVELKGLHESGALSDEQYKKAVDKIVS"
     gene            complement(1520..1738)
                     /locus_tag="PKV30_02165"
     CDS             complement(1520..1738)
                     /locus_tag="PKV30_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29057.1"
                     /translation="MVIAGVMLANGVGFGIATAISTYVFGIAWVTVGALVLPGALLVV
                     MAAKTARPVDDVSLSVALAYKLLERLGK"
     gene            complement(1806..2309)
                     /locus_tag="PKV30_02170"
     CDS             complement(1806..2309)
                     /locus_tag="PKV30_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29058.1"
                     /translation="MTTQVPTSRFVDMALSFAGGDGGNLDSEVWFCGFEWGNSHGIDI
                     EKTIDTEFQPIPEPTSWAHKDFEGSWNTNYNRKICLFLKYFYAIDWEGMSYDEFVANH
                     QILYPDGLGFKLNLLPVRFRNRESLAWAKAIEAASGFETFDDYRSWCVTHRGAFFRAL
                     LSKHKDR"
     gene            complement(2452..3162)
                     /locus_tag="PKV30_02175"
     CDS             complement(2452..3162)
                     /locus_tag="PKV30_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HRL29059.1"
                     /translation="MSSRAARLLQLLEHLRTYRHPVAGAQLAQAQGVSLRTLYRDIAT
                     LREQGAQIEGDPGVGYVLRPGFTLPPLMFSADELEALVLGARWVASHAADPELADAAR
                     HAVQRVSGVLPSALRLAIDTNGLFVPARSDSPTPAPWLPVLRRAIRDEHALQLDYRDE
                     GSAVTQRRIWPFAMAFFDQARLLAAWCELRQDFRHFRADRVLNLSDSGERYPARRHAL
                     IQRWREQMRHFSRTDVHC"
     gene            complement(3175..4194)
                     /gene="thiL"
                     /locus_tag="PKV30_02180"
     CDS             complement(3175..4194)
                     /gene="thiL"
                     /locus_tag="PKV30_02180"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRL29060.1"
                     /translation="MGEFELIERFFKRNQPPVLANQAQAAINSIAFGIGDDCALLQPA
                     PGMQLAISTDMLVEGRHFFADVDPESLGHKALAVNLSDLAAMGAEPLACTLALALPPE
                     RARDEAWLGAFARGLFALADEHGCALIGGDTTAGPLNLCITVFGQLPAGQALRRDGAR
                     AGDDVWVSGTLGDARLALGALRGEWPLPPDVLDAARQRLERPTPRLALGLALRGIASA
                     AADVSDGLAGDLGHILQASGVGAVLDFEDASFLIAARADLTSADGLFDSAFLLKTALT
                     GGDDYELVFTAPPTARSAVQAASERAATPVTRIGRIEAAPGLYLADADGRTAPLAARA
                     FDHFA"
     gene            complement(4213..5061)
                     /locus_tag="PKV30_02185"
     CDS             complement(4213..5061)
                     /locus_tag="PKV30_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRL29061.1"
                     /translation="MTLRIGISARLLHNPPPGLGLPTKRLQFLESGMATWIMSHGVVA
                     LMIPFIDQSAPLQRKRPPLNDLVANLDGLVLQGGIDISPETYGQKPWVEKAEYDPIRD
                     DYELGLLRGFIEAEKPVLGICRGCQLLNVYFGGTLIQDIPTQWPGAILHNDTHRYDRL
                     IHELHFMPGSRLSDIYGYEPRRVTSIHHQCVDQLGKGLVLEARSPIDLVPEGIRHTGY
                     PFVMGVQWHPEFHMNGFDPGDGVLDSGPLMMAFLKAAARRAGRVRRLADGVARVRERA
                     SALGGR"
     gene            complement(5313..6437)
                     /locus_tag="PKV30_02190"
     CDS             complement(5313..6437)
                     /locus_tag="PKV30_02190"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HRL29062.1"
                     /translation="MTLQAFGQSEALTLGVELELQLVNTNDYDLAHYADDMLRLMARR
                     KLPGSVVPEMTAGMIEISTDVCATWRDALAQLTQIRDALVQCADKLNIAVVGGGTHPF
                     QQWHQQRIYDKPRFHELSALYGYLTKQFTIFGQHVHVGCPDADTALYTLHCMSRYIPH
                     FIALAASSPYVQGQDTAFDSARLNSVFAFPLSGRAPCVLTWDEFGQYFDKMTRTGVVK
                     SMKDFYWDIRPKPEYGTIEVRVFDTPLTVERAAALAGYVQSLAAWFQQERPFAPVEDD
                     YLVYGYNRFQACRFGLDAVYVDPASGEHLPLRDHILATMDRIQPFAEPCQASGALAML
                     RQEVQDNRNDARWLRERQSEERLLGEVIRQAAQRFRGRAA"
     gene            complement(6434..7696)
                     /locus_tag="PKV30_02195"
     CDS             complement(6434..7696)
                     /locus_tag="PKV30_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRL29063.1"
                     /translation="MHDAIAFWPAALQSAPGPLTLEWALLLALAALAGHLVQRFTGLP
                     KILGYAVFGTAVGLAGFAGGAWPLHGVGLYLLELGIAVVLFEAGARLSLRWFRHNPMV
                     LVQSLIEALLTFFAAYLLLRALELDVPVARALAVVSVAASPAVLMRVVSDLRASGPVT
                     DRAIALATLNTLYALTAGTAMLRSIDRGDTTMLASLASSAAVLGVSLLVGALLAALLV
                     GAFKLLHPTSQDTAIVILALIGACTAVTTPLGGSAPLATLLAGIVLKQLQPRPWVWPR
                     QLGTAAAMLNVLMFVLVSLMAAQADWHGALTWAALALVAARMAAKLLSLLLTGPASGL
                     SLRQSLWVGVALVPMSSVALLLTSQFVVASHSVGARVAAIALPVILITELLGAVLVGL
                     ALVRAREAVKPWRHSDPAAADSQPGPLA"
     gene            complement(7799..9859)
                     /locus_tag="PKV30_02200"
     CDS             complement(7799..9859)
                     /locus_tag="PKV30_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HRL29064.1"
                     /translation="MGTSQLELIELMRALDAEAPLAERNLWLIRLLAWVRGDSDDVDM
                     AVARMRMLIDAAEVRPDWLHQWQDWWREFLTTMDATALLADLGFAPRGSFPSELVHRL
                     RRKLLPATPETTDLSELFGLMFPTELDAKWLRALDEPTLQRLRTTLFRAEGHSTQAGG
                     GYALRTLLDAMAYATGQISAIGQSADVRLRMSREAREQRPFLDLPIVFETLRQAVLTH
                     GRQSAPALVAAAELRSQLDACRAAAATVYAHLGEHGISVHIEFQLRQLRQRILRVKAL
                     LLCLDTDAPAQATAQLLSHLVRVNRDSLSMRALLASSTELLAAKVTERNAETGEHYIT
                     RTPRDYLRMLGAASGGGVVLAFTTWIKLGLGMLGLSAFWGGLYAGLNYAVSFVIIMLA
                     HWTVATKQPAMTAPAMAARLKNVTTPEAVENFVDEMVYLLRSQYAAILGNVGLVIPAA
                     IGIGVVVQWLGWDGVLSASHAREILAHHHLLGPTALFAAATGGLLFLSSIVAGWVENW
                     FVFHRLDSVMAYDPRMTRWLGAARAARWGAYWRDHISGYTANISLGLMLGLVPPIIGF
                     FGVPFEVRHVTLVAGQIAASAHKLGAQVLHQPAFGWAVAGIVITGLMNLTFSFTLAFR
                     LALTAQNVPAVDRRRVYIALAWRALRRPWSFVLPLRVRRPASADAADAPPAAEKVTKM
                     DASA"
     gene            complement(10011..10406)
                     /gene="apaG"
                     /locus_tag="PKV30_02205"
     CDS             complement(10011..10406)
                     /gene="apaG"
                     /locus_tag="PKV30_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRL29065.1"
                     /translation="MPKYQLSVEVEPQYLPDQSEPASELYGFAYTITVHNTGEVPAQL
                     ISRHWIITDARGHVEEVKGLGVVGQQPLLGPGEAFQYTSGCRLRTPSGTMHGTYFCVA
                     ADGERFEADIPMFVLDAGGQAGVSARVLH"
     gene            10460..11143
                     /gene="rpe"
                     /locus_tag="PKV30_02210"
     CDS             10460..11143
                     /gene="rpe"
                     /locus_tag="PKV30_02210"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRL29066.1"
                     /translation="MSRTYRIAPSILSADFARLGDEVKNVIAAGADWIHFDVMDNHYV
                     PNLTFGPMICQAIKPHAVRADGSPVPMDVHMMVQPVDALAAAFAQAGADFISFHPDAT
                     PHPHRSVQAIRAAGAKPGLVFNPAAPLDVLDWLIDDIDLILIMSVNPGFGGQSFIDSA
                     LRKVEAARKRIEASGRDIRLEVDGGIKPANIRQVADAGADTFVAGSAIFGQKDYAAVI
                     DEMRRELAA"
     gene            complement(11261..11695)
                     /locus_tag="PKV30_02215"
     CDS             complement(11261..11695)
                     /locus_tag="PKV30_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRL29067.1"
                     /translation="MTLNKTARALLAAAALSLAAAGAWAQDKVVYHIDDAATQATKGL
                     RNIRNHLDVEPKTQIMVVTHADGVDFLMDGAKDAKNPNIDYAALVSALKARGVKFEVC
                     EITLRNRNLKKEQFNMDAEFTPSGVARIAKLQTRDGYAYIKP"
     gene            11717..12451
                     /gene="gph"
                     /locus_tag="PKV30_02220"
     CDS             11717..12451
                     /gene="gph"
                     /locus_tag="PKV30_02220"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655723.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HRL29068.1"
                     /translation="MSALKVAQPIEACIIDLDGTLVDTLGDFEAALNQMLAALGRAPV
                     TRAQIALMVGKGSEHLIKSALALDQRAPAAIETQADAALQAAAWQHYQDAYLRINGLH
                     SRIYPGAAEGLQALADLGLPLACLTNKPLGFARPLLTSKGLVGFFSHVFGGDSFVRKK
                     PDPLPLIETCRALGTAPARTLMIGDSSNDAAAARAAGCPVVLMTYGYNHGEPVQGVDA
                     DGLADALTEVAAALAAHRHNGLQKER"
     gene            12448..13416
                     /locus_tag="PKV30_02225"
     CDS             12448..13416
                     /locus_tag="PKV30_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRL29069.1"
                     /translation="MTAVNLDHELLRRAVAQPEVLHWQTWAALAPGPSLLVLGAVHGN
                     EVCGAHGIVRAIDDLAHGRIVLRRGRLTLVPVANPLAFKQNTREGERNLNRHFLPQPA
                     PQDYEDRITNQLTPLLAQHDVLLDLHSFHTPGAPFAMVGPRNNPGPREPFARAAEEMA
                     LARALGAPQVVEGWLDVYDRAALARGEEPGDDGIGTNEYMRSQGGYAVTIECGQHEDP
                     RAISVAERAIHGALALLGLADVPAPPRFGGPAARLKDVVLRESPGDQLVRDWHSFDAV
                     RAGDVIGVREDGTEVRAPYDGCVLFPHPEADVGQEWFYLAVFDAAA"
     gene            complement(13489..14052)
                     /locus_tag="PKV30_02230"
     CDS             complement(13489..14052)
                     /locus_tag="PKV30_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRL29070.1"
                     /translation="MLGGAALMSSAGARAVELAGVQFPQQVNVAGKPLQLNGAGVRYK
                     AVFKVYAAGLYVPAKTASATEAVATDTPKRLSITMLRDIESAELGKMFERGMEENAER
                     GSMVRLLPGIMRMSQVFSDHKKLKAGESFQVDWVPGTGAVLTIKGHAVGEPFREPEFF
                     QTLMRIWLGAKPADWKLKDSLLGKEDA"
     gene            complement(14206..15039)
                     /locus_tag="PKV30_02235"
     CDS             complement(14206..15039)
                     /locus_tag="PKV30_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRL29071.1"
                     /translation="MLIQIGYDIELAVNAPTTLLCALHVHPSRAHDLQAPEQFEVEPA
                     LPISTYQDGFDNTITRVDVPPGVASVRFTNHAVVADSGLPDPVVPDARQHPLHELPLE
                     VFRFLLPSRYCEVDSELMQLAWNQFGNTPEGWARVQAIVDYTHHHIRFDYMQARANRT
                     ALQGWQEGVGVCRDYAHLAVTLCRCMNIPARYVTGYLGDIGVPPAAAPMDFAAWMEVY
                     LGGQWHTFDARNNARRIGRIVMARGRDATDVPIAMVFGWQQLNRFDVVTDEIKDTAVV
                     A"
     gene            15450..>16179
                     /locus_tag="PKV30_02240"
     CDS             15450..>16179
                     /locus_tag="PKV30_02240"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655421.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRL29072.1"
                     /translation="MITELEFKSLAGQGYNRIPLMAEAFADLETPLSLYLKLASGAGG
                     SGAGRYSFLLESVIGGERFGRYSFIGLPARTILRASGFGANARTEVVTDAKVVETAEG
                     NPLDFIADYQKRFKVALRPGLPRFCGGLAGYFGYDTVRHIEKKLEKTCPPDPLGLPDI
                     LLMHSEEVAVIDNLSGKLYLIVYADPAQPEAYSNAKKRLKTLREQLRYSVSVPQVKAT
                     QGFPAEREFAKADYLKAVERAKELI"
BASE COUNT         2913 a         5244 c         5256 g         2766 t
ORIGIN      
        1 gtgtcggaat tctttgcgcc agacttcggc tttaattctt tcccatcgca cgaccaattt
       61 gtagggatct ggataatatt tgtcatatag gttttccttt gcgctgtagg atgggctggt
      121 cacatactcg acataattca gaaagtcgtt tgacgattct gttccaagcg ccgacaggcg
      181 atcacggtgc aatcatcggg gatgcctcgg cggagcacct ccgtacgcag tgcacgaacg
      241 ttgaccgcca agcttgattc gctgttccag agggccagtc ttttctcttc gtcaagacag
      301 tcgtgcaatc catcggtggc aagcaataga acgtcgccgc cctccactcg gctctgttgc
      361 acgtgcaatt tggggtagtc ttcaggctct gcaaaccggc cgatttcatc ctggctgaca
      421 gcaaatgccg taactcagca tcggggtgat gggcggcagc ggtttggatg gattcagagg
      481 ttttgaaaat ctccataggc agaactcctt tcaggttgaa atgggatggg caaaagaaaa
      541 gcgccctggg attgctccga gggcgcttga tataggactc taatttcagg acactatctt
      601 atctactgct ttcttgtact gctcgtcgct caaagcacca gattcatgga gcccttttaa
      661 ttcaacaagc tgtcgcgtca ttgaatttga atcaatctct ggcttatgct caagcatttt
      721 ggtagatttc acaaataatg gtgagagcag tattctcatc ttgtcaggct ctgtatcaga
      781 agacaaaagt atcaattccg cgtcttccag taataacctg acgtacgttt tggtgctgct
      841 gtgcgtggtc aatatatttg ccaaagtggc aatgcccatg ccaaccaagg caccttcccc
      901 aaagccaccc ccagcaaagc caccgccaga cgttacttta cccgggccac ctatgtctat
      961 cgacctgatc tccccatatg gcaatgatat tttcttgatc cttttgaaat catagaaaac
     1021 caatgcatca cttgcaattt ttacgtcaat agacgtgtca tgcatgggct cccaccctgc
     1081 acctccaaca acttttacac gtaacttctt tgccccatca ggcaataaat ttaaatccat
     1141 ctttaagttt ataatttttt gattttcgct agccttttgg gactgcttaa tctccctcat
     1201 gacggcatcc tcctgttttt tcctcgccat ctcctcttta gcttcattca ttttttgccg
     1261 ctcctcctcc gtgaaatatc tttcaacatt catcgatccg caagaggtgc agtggttttt
     1321 cctcatcaag aaatacagaa tcatcaaagc aggtatgcct acataaggga gcgccacagc
     1381 aaagattgct ccaacaaacc atacaggtag aagcagatac catgcgccat gcaacttttt
     1441 ctttgcaggt ccgatctgcg agcaatcccc gcatttaaac aatgcttcgc ctttcataaa
     1501 cctcctatgt aaaaaatcat catttaccca atcgctccag cagcttgtaa gccagagcga
     1561 cggacagaga aacgtcatcc acagggcggg ctgtcttggc tgccatcacg accaatagcg
     1621 cgccgggaag caccaaggcc cctaccgtga cccaggcgat accaaacaca taagtggaaa
     1681 tggctgtggc aatgccgaat ccgactccat tggccagcat tactccggca atgaccatca
     1741 aagcacccac accacccagg ccagtggtgg acagcagttc atagatttgc cggaagttgc
     1801 gtgaatcacc tgtctttatg tttgctcaac aatgccctga aaaatgcgcc acggtgggtg
     1861 acgcaccagc tgcggtagtc gtcaaatgtt tcaaaccccg aggcagcctc tattgccttg
     1921 gcccaggcca aggactcgcg gttgcgaaag cggaccggca ggagattgag tttgaagcca
     1981 aggccatctg gataaagaat ttgatggttg gcaacgaatt catcgtagct catgccctcc
     2041 caatcaatgg cgtaaaaata tttgaggaac agacagattt ttctgttgta gttggtgttc
     2101 cagctgcctt cgaagtcctt gtgtgcccag gaggtcggtt ccgggatcgg ttgaaactcc
     2161 gtatcgatgg ttttttcaat atcgatgccg tgcgagttac cccactcaaa gccgcagaac
     2221 cagacctccg aatcaaggtt tcctccgtca cctccggcaa aggacaacgc catatcgacg
     2281 aatcgcgacg tgggtacctg agtagtcatg gcaaagctcc aataaaaaaa ctgcaaaccg
     2341 ttgcatgcgt gcagggacag aaactaccgc ccgatgcgct gtgcgaataa atggcaggat
     2401 cacttgacgg ccaagtgttc aaaggttaaa cgttgcctgt gtcagattgt gtcagcagtg
     2461 gacgtcggtt cgtgaaaaat gccgcatctg ctcgcgccag cgctgaatca gcgcgtgccg
     2521 gcgtgccggg tagcgctcgc cgctgtcgga caggttcagc acccgatcgg cgcggaagtg
     2581 ccgaaaatcc tgccgcagct cgcaccatgc tgcgagcagc cgcgcctggt cgaagaaggc
     2641 catggcaaac ggccagatgc ggcgctgcgt gacggcgctg ccctcgtcgc ggtagtccag
     2701 ctgcagtgca tgttcgtcac ggatggcgcg gcgcagcacg ggcagccagg gcgcgggcgt
     2761 tggtgaatcg ctgcgcgcgg gcacaaacaa gccgttggtg tcaatcgcca ggcgcaacgc
     2821 cgaaggcagc acgccactca cgcgctgcac ggcgtggcgc gcggcatcgg ccagttcagg
     2881 gtcggccgcg tggctggcga cccagcgcgc gcccagcacc agcgcctcca gctcgtcggc
     2941 cgagaacatc agcggcggca gcgtgaaacc ggggcgcagc acgtagccga cgccggggtc
     3001 gccctcgatc tgcgcgcctt gctcgcgcag ggtggcgatg tcgcggtaca gcgtgcgcag
     3061 gctcacgccc tgcgcttgcg ccagttgggc gccggcgacg gggtggcgat acgtgcgcag
     3121 gtgttcgagc agttgcagca ggcgggcggc gcggctggac atggcgcgcc gcgatcaggc
     3181 gaaatggtcg aaggcgcgcg cggccagcgg cgccgtgcgg ccgtcggcgt cggccagata
     3241 caggccgggt gcggcttcga tgcggccgat gcgggtcacc ggcgtggcgg cgcgctcgct
     3301 ggcggcttga acggcgctgc gggcagtggg cggggcggtg aagaccagtt cgtagtcgtc
     3361 gccgccggtc agcgctgtct tgagcagaaa cgctgaatca aataggccgt cagcgcttgt
     3421 tagatcggcg cgagcagcta tcaaaaaaga agcatcttcg aaatcgagca cagcgccgac
     3481 gccgctcgcc tgaaggatgt ggcccaggtc gcccgccagg ccgtcgctca cgtcggcggc
     3541 ggcgctggcg atgccgcgca gggccaggcc aagggcgagg cgcggcgtgg ggcgttccag
     3601 ccgctggcgc gcggcgtcca gcacgtcggg cggcagcggc cattcaccgc gcagcgcgcc
     3661 cagcgccagc cgcgcgtcgc ccagcgtgcc gctgacccac acatcgtcgc ccgcgcgcgc
     3721 gccatcgcgc cgcagcgcct gaccagccgg cagctggccg aagacggtga tgcagaggtt
     3781 gagcgggccg gccgtggtgt cgccgccgat cagagcgcag ccgtgctcgt cggccagcgc
     3841 gaacaggcct cgcgcgaagg cgccgagcca ggcttcgtcg cgcgcgcgct cgggtggtag
     3901 ggcgagggcc agcgtgcagg cgagcggttc ggcgcccatg gcggccaaat cgctcaggtt
     3961 gacggcgagc gccttgtggc ccagcgattc gggatcgacg tcggcgaaaa aatggcggcc
     4021 ttcgaccagc atgtcggtgc tgatggccag ctgcatgccg ggcgcgggtt gcagcagcgc
     4081 gcagtcgtcg ccgatgccga aagctattga attgatagct gcttgcgctt gattggcaag
     4141 cactggcggc tgatttcgct tgaaaaagcg ctcgatcagc tcgaactcgc ccatggcgca
     4201 acccgcgacc agtcagcggc cgccgagcgc gctggcgcgt tcgcgcacgc gcgccacgcc
     4261 atcggccagg cgccgcacgc ggccggcgcg gcgcgcggcg gctttcagaa aggccatcat
     4321 cagcgggccg ctgtcgagca cgccgtcgcc ggggtcgaag ccgttcatgt gaaattcggg
     4381 gtgccactgc acgcccatca cgaagggata gccggtgtgg cggatgcctt cgggcaccag
     4441 gtcgatgggg ctgcgcgctt ccagcaccag gcccttgccc aattgatcga cgcactggtg
     4501 gtggatgctg gtgacgcggc gcggttcgta gccgtagatg tcggacaggc gcgagccggg
     4561 catgaagtgc agttcgtgga tcagccggtc gtagcggtgc gtgtcgttgt gcaggatggc
     4621 gcccggccat tgcgtgggga tgtcctggat cagcgtgccg ccgaagtaca cgttgagcaa
     4681 ctggcagccg cggcagatgc cgagcacggg cttctcagcc tcgatgaagc cgcgcagcag
     4741 gcccagttcg tagtcgtcgc ggatggggtc gtattcggcc ttttcgaccc agggtttctg
     4801 accgtaggtt tcggggctga tgtcgatgcc gccttgcagc accaggccgt cgagattggc
     4861 caccaggtcg ttgagcggcg ggcgcttgcg ctgcagcggc gcgctttggt cgatgaaggg
     4921 aatcatcaac gccaccacgc cgtgcgacat gatccaggtg gccatgccgc tttccagaaa
     4981 ctgcagccgc ttggtaggca gccccaggcc gggcggcggg ttgtgcaaca ggcgggcaga
     5041 gatgccgatg cgcagggtca tggtgggtgc ttgtgaatca aacagggcgc tggcgctgat
     5101 ggggcaagcg cgagcagcta tgatttttgc atgacgcgcc tggcggcgca tgacctcggc
     5161 ctgcggcctt cgatgacggc gcggcgcgcc atgacctcaa gccctgggtc attccgtcat
     5221 tgcagcgaag cgaagtcatt tcgtcatcag atcgaccgtt ggcgcctacc actcaagcgc
     5281 gagcagctat caaatataga gtatcgagcg tttcacgcag cgcgccccct gaatcgctgc
     5341 gcggcctggc ggatcacttc gcccagcagg cgctcctcgg actgccgctc gcgcagccag
     5401 cgcgcgtcgt tgcggttgtc ctgcacttcc tggcgcagca tggcgagtgc gccgctcgcc
     5461 tggcagggtt cggcaaaggg ctggatgcgg tccatggtgg ccaggatgtg atcgcgcaac
     5521 ggcaggtgct cgccgctggc cgggtcgacg tacacggcgt cgagcccaaa gcggcaggcc
     5581 tgaaagcggt tgtagccgta gaccaggtag tcgtcttcga cgggcgcaaa cggccgctct
     5641 tgctggaacc acgccgccag cgactgcacg tagccggcca gcgcggctgc gcgctccacg
     5701 gtcagcgggg tgtcgaacac gcgcacttcg atggtgccgt attcgggctt ggggcggatg
     5761 tcccagtaaa agtccttcat cgacttgacc acgccggtgc gtgtcatctt gtcgaaatac
     5821 tggccgaact cgtcccaggt cagcacgcag ggcgcgcggc ccgacagcgg aaaggcaaac
     5881 accgaattga gccgcgccga atcaaacgcc gtgtcctgcc cttgcacgta ggggctgctc
     5941 gccgccagcg caatgaagtg cgggatgtag cggctcatgc agtgcagcgt gtagagcgcg
     6001 gtgtcggcgt cagggcaccc cacgtgcacg tgctggccga agatggtgaa ctgcttggtc
     6061 aggtagccgt agagcgccga cagctcgtga aagcgcggct tgtcgtagat gcgctgctgg
     6121 tgccactgct gaaacgggtg ggtgccgccg cccaccacgg cgatgttgag cttgtcggcg
     6181 cactgcacca gcgcgtcgcg gatctgggtc agctgcgcca gcgcgtcgcg ccaggtggcg
     6241 cagacgtcgg tggagatttc gatcatgccg gccgtcattt ccggcaccac cgagccgggc
     6301 agcttgcgcc gcgccatcag gcgcagcatg tcgtcggcgt agtgcgccag gtcgtagtcg
     6361 ttggtgttga ccagttgcag ttccagctcg acgccgagcg tcagcgcctc ggattgacca
     6421 aaagcttgca gcgtcatgcc agcggtcccg gttgactgtc ggcggcggcc ggatcgctgt
     6481 ggcgccacgg cttgacggct tcgcgcgcgc gcaccagcgc caggccgacc aacaccgcgc
     6541 ccagcagttc ggtgatcaaa atcaccggca gcgcaatcgc cgccacgcgc gcgcccacgc
     6601 tgtgcgaggc caccacgaat tgcgaggtca gcagcagcgc caccgacgac atcggcacca
     6661 gcgccacccc cacccacagc gactgccgca gcgacagccc gctggccggc ccggtcagca
     6721 gcaggctgag cagcttggcc gccatgcgcg cggcaaccag cgccagcgcg gcccaggtca
     6781 aggcgccgtg ccagtcggcc tgcgccgcca tcaaggacac cagcacgaac atcagcacgt
     6841 tgagcatggc cgccgccgtg cccagctggc gcggccagac ccagggccgc ggctgcagtt
     6901 gcttgagcac gatgcccgcc agcagcgtgg ccagcggggc cgagccgccc agcggcgtgg
     6961 tgacggccgt gcaggcgccg atcagcgcca ggatgacgat ggccgtgtcc tggctggtcg
     7021 ggtgcagcag cttgaaggcg ccaaccagca gcgccgccag cagcgcgccg accagcagcg
     7081 acacgcccag caccgccgcc gacgaggcca gcgacgccag catggtggtg tcgccgcggt
     7141 cgatgctgcg cagcatggcg gtacccgccg tcagcgcgta cagcgtgttg agcgtggcca
     7201 gcgcgatggc gcggtcggtc accgggccgc tggcgcgcag atcggacacc acgcgcatca
     7261 gcaccgcggg cgatgccgcc accgacacga cggccagcgc gcgcgccacc ggcacgtcca
     7321 gctccagtgc gcgcagcagc aggtaggcgg caaaaaaggt cagcagcgcc tcaatcaggc
     7381 tttgcaccag caccatcggg ttgtggcgga accagcgcag cgaaagccgc gcgccggctt
     7441 cgaacagcac cacggcgatg cccagctcca gcaggtacag gccgacgccg tgcagcggcc
     7501 aggcgccgcc ggcaaaaccg gcaaggccga ccgcggtgcc gaacaccgcg tagccgagga
     7561 tcttgggcag cccggtaaag cgctgcacca ggtgcccggc cagcgccgcc agcgccagca
     7621 gcagcgccca ttcaagcgtc agcgggcccg gcgcggattg cagggcggcg ggccagaaag
     7681 cgatggcgtc atgcagcaaa acgaaagggg tccttggttg tgttcaggtt catgctaacc
     7741 gcttcaggcg gggtgaccag agggcttgtc aaaaaagata gctgcccgcg ctttccagtc
     7801 aagcgctggc gtccattttt gtcacttttt cagcggcggg tggcgcgtcc gccgcgtccg
     7861 ccgacgccgg gcgccgcacc cgcagcggca gcacgaagga ccacggccgg cgcagcgcgc
     7921 gccaggccag cgcgatgtac acgcgccgcc gatccaccgc cggcacgttt tgcgccgtca
     7981 gcgccagcct gaaggccagc gtgaaactga acgtcaggtt catcaagccg gtgatgacga
     8041 tacccgccac cgcccagcca aacgccggct ggtgcagcac ctgcgccccc agtttgtgcg
     8101 ccgacgccgc gatctgcccg gccaccagcg tgacgtgacg cacctcgaac gggacgccga
     8161 agaagccgat gatcggcggc accagcccca gcatcagccc gagcgagatg ttggcggtgt
     8221 agcccgagat gtggtcgcgc cagtaggcac cccagcgcgc cgcgcgcgcg gcgcccagcc
     8281 agcgtgtcat gcgcgggtcg taggccatca ccgaatcaag gcggtgaaag acgaaccagt
     8341 tctccaccca gccggcgacg atgctggaca ggaacagcaa gcccccggtg gccgccgcaa
     8401 acagcgcggt cggccccagc aggtggtggt gcgccaatat ctcgcgtgcg tgggaggcgc
     8461 tcagcacgcc gtcccagccc agccactgca ccaccacgcc gatgccgatg gccgccggaa
     8521 tcaccagccc cacgttgccc aaaatggcgg cgtactgcga gcgcagcagg tacaccatct
     8581 cgtcgacaaa attttccacc gcttcgggcg tcgtcacgtt cttgaggcgc gccgccatgg
     8641 ccggcgcggt catcgccggc tgcttggtcg ccaccgtcca gtgcgccagc atgatgatga
     8701 cgaacgacac cgcgtagttc agccccgcgt acaacccgcc ccagaacgcc gacagtccga
     8761 gcatgcccag tcccagcttg atccaggtgg tgaaggccag caccacgccg ccgcccgatg
     8821 ccgcgcccag catgcgcagg tagtcgcgcg gcgtgcgggt gatgtagtgc tcgccggttt
     8881 cggcgttgcg ctcggtcacc ttggcggcca gcagttcggt gctgctggcc agcagcgcgc
     8941 gcatgctcag gctgtcgcgg ttcacgcgca ccaggtgcga caacagctgc gccgtagcct
     9001 gcgccggcgc atcggtgtcc aggcacagca gcagcgcctt gacgcgcagg atgcgctggc
     9061 gcaactggcg cagctggaat tcgatgtgca ccgaaatgcc gtgttcgccc aggtgcgcat
     9121 acaccgtggc cgcggcggcg cggcaggcgt cgagctggct gcgcagctcg gccgcggcca
     9181 ccagcgccgg cgcgctctgg cggccgtgcg tcagcaccgc ctggcgcagt gtctcgaaga
     9241 cgatcggcag atcgagaaac ggccgctgct cgcgcgcctc gcgcgacatg cgcagccgca
     9301 cgtcggccga ctggccgatg gcgctgatct ggccggtggc gtaggccatg gcgtccagca
     9361 gcgtgcgcag cgcatagccg ccgccggcct gggtactgtg gccctcagcg cgaaacagcg
     9421 tggtgcgcag gcgctgcagc gtcggctcgt ccagcgcgcg cagccacttg gcgtccagtt
     9481 cggtcggaaa catcagcccg aacagctcgc tcaggtcggt ggtttcgggc gtggccggca
     9541 gcagtttgcg gcgcagccgg tgcaccagct cgctcggaaa cgagccgcgc ggcgcaaagc
     9601 ccaggtcggc cagcagcgcc gtggcgtcca tggtggtcag aaattcacgc caccagtcct
     9661 gccactgatg cagccagtcg ggccgcacct cggccgcgtc gatcagcatg cgcatgcgcg
     9721 ccacggccat gtccacatcg tccgaatcgc cgcgcaccca ggccagcagg cgaatcagcc
     9781 acaggttgcg ctcggccagc ggcgcctcgg catcaagcgc ccgcatcagc tcgatcaact
     9841 caagttgcga cgtccccatc tcaagcgctc aaaaaccctt gcgccacaag gctgcgaagc
     9901 aggccaaacc gagggccgcg gagcaggcca cgcgggaacg ccgtggaacg ggcttggccc
     9961 ggccactggc gttgtcccct gggggaaggc gccgcaggcg acacaggggg ttagtgaagt
    10021 acgcgcgcgc tgaccccagc ctggccgccg gcgtcgagca cgaacatggg gatatcggcc
    10081 tcaaagcgct cgccatcggc cgccacgcag aaataggtgc cgtgcatggt gccgctgggc
    10141 gtgcgcaggc ggcagccgct ggtgtactga aacgcctcgc ccggccccaa cagcggctgc
    10201 tggccaacca cgcccaggcc cttgacttct tcgacgtggc cgcgcgcgtc ggtgatgatc
    10261 cagtggcgcg aaatcagctg cgccggcacc tcgcccgtgt tgtgcacggt gatggtgtac
    10321 gcaaagccat acagctcgct ggccggttcc gactgatcgg gcaggtattg cggttcgacc
    10381 tcgacactga gctggtattt gggcatgcgc ggatgttaac ggcggcgcct ggcgcggtgc
    10441 gtaggcgaaa atcccccgca tgagccgcac ttaccgcatc gccccgtcca tcctgtcggc
    10501 cgactttgcc cgtctgggcg atgaggtgaa gaacgtcatc gccgccggcg ccgactggat
    10561 tcacttcgac gtcatggaca accactacgt gcccaacctg accttcgggc cgatgatttg
    10621 ccaggcgatc aagccgcacg ccgtgcgcgc cgacggcagc ccggtgccga tggacgtgca
    10681 catgatggtg cagccggtgg atgcgctggc cgcggccttt gcgcaggccg gcgccgattt
    10741 catcagcttt caccccgacg ccacgccgca cccgcaccgc agcgtgcagg cgattcgcgc
    10801 ggccggcgcc aaaccgggcc tggtgttcaa ccccgccgcg ccgctggatg tgctggactg
    10861 gctgatcgac gacatcgacc tgatcctgat catgagcgtc aaccccggtt ttggcggtca
    10921 gtcctttatc gatagcgcct tgcgcaaggt ggaggcggct cgcaagcgga ttgaggcatc
    10981 gggccgcgac atccggctgg aggtcgatgg cggcatcaag cccgccaaca tccgccaggt
    11041 ggccgatgcc ggggccgaca cctttgttgc cggcagcgcg atctttggcc agaaggacta
    11101 tgcggcggtg atcgacgaga tgcggcgcga attggccgcg tagggtgggt gcttgtaccc
    11161 accgtggacc gatgcgctgc ccgcgtgggt acaagcaccc accctacgaa aatcatagct
    11221 gcccgcgctt gtcacacaag cgcaggagcc gttttttgcc tcaaggcttg atgtacgcgt
    11281 agccgtcgcg cgtctgcagc ttggcgatgc gcgccacgcc cgagggcgtg aactcggcgt
    11341 ccatgttgaa ctgctctttc ttcaggttgc ggttgcgcag cgtgatctcg cacacctcga
    11401 acttcacgcc gcgcgccttc agagcactga ccagcgcggc gtagtcgatg ttggggttct
    11461 tcgcgtcctt ggcgccatcc atcaaaaagt ccacgccgtc ggcgtgcgtc accaccatga
    11521 tctgcgtctt gggttccacg tcgaggtgat tgcggatgtt gcgcagcccc ttggtggcct
    11581 gcgtggcggc gtcgtcgatg tggtacacca ccttatcctg cgcccaggcg ccggcggcgg
    11641 ccaggctcaa tgcggcggcg gccagcagcg cgcgggcggt cttgttcaac gtcatgtctt
    11701 gtctcctgga tcgttcatgt cggcgctgaa agtagcacag cccatcgagg cttgcatcat
    11761 cgacctcgac ggcacgctgg tcgatacgct gggcgatttc gaggccgcgc tgaaccagat
    11821 gttggccgcg ctcggccgcg cgcccgtcac gcgcgcgcag atcgcgctga tggtcggcaa
    11881 gggctcggaa catttgatca aatcggcgct ggcgcttgac cagcgtgcgc cagcagctat
    11941 cgaaacgcaa gcagacgcgg ccttgcaagc cgccgcctgg cagcactacc aggacgccta
    12001 cctgcgcatc aacggcctgc acagccgcat ctaccccggc gcggccgaag gcttgcaggc
    12061 gctggccgat ctgggcctgc cgctggcctg cctgaccaac aagccgctgg gttttgcgcg
    12121 cccgctgctg acgtccaagg ggctggtcgg atttttcagc cacgtctttg gcggcgacag
    12181 ctttgtgcgc aagaagcccg atccgctgcc gctcatcgaa acctgccgcg cgcttggcac
    12241 cgcgcctgcg cgcaccttga tgatcggcga ctccagcaac gacgccgccg ccgcgcgcgc
    12301 tgccggctgc ccggtggtgc tgatgactta cggctacaac cacggcgagc cggtgcaggg
    12361 cgtcgatgcc gacggcttgg ccgatgcgct gaccgaggtc gctgccgcgc tggccgcgca
    12421 tcggcacaat gggctgcaga aagagagatg actgcggtga atctggatca tgaactgctg
    12481 cgccgcgccg tcgcgcaacc cgaagtgctg cactggcaga cctgggccgc gctggcgcct
    12541 gggccgtcgc tgctggtgct gggcgcggtg cacggcaacg aggtgtgcgg cgcccacggc
    12601 atcgtgcggg ccatcgacga cctggcgcac gggcgcatcg tgctgcggcg cgggcggctg
    12661 acgctggtgc cggtggcgaa cccgctcgct ttcaagcaga acacgcgcga gggcgagcgc
    12721 aacctgaacc gccactttct gccgcagccc gcaccgcaag actatgaaga ccgcatcacc
    12781 aaccagctga cgccgctgct ggcgcagcac gacgtgctgt tggatttgca ctcgttccac
    12841 acgccgggcg cgccgtttgc catggtcggc ccgcgcaaca accccggtcc gcgcgagccg
    12901 tttgcccgcg ccgccgagga aatggcgctg gcgcgcgcgc tgggtgcgcc gcaggtggtt
    12961 gaaggctggc tcgacgtgta cgaccgcgcc gcgctggcgc gtggcgagga gccgggcgac
    13021 gacggcattg gcaccaacga atacatgcgc agccagggcg gctacgcggt caccatcgaa
    13081 tgcgggcagc acgaagaccc gcgggcgatc agcgtggccg agcgcgcgat ccacggcgcg
    13141 ctcgcgctgc tgggcctggc cgacgtgccc gcgccgccgc gctttggcgg ccccgccgcg
    13201 cggctgaaag acgtggtgct gcgcgaatca cccggcgacc aactggtgcg cgactggcac
    13261 agctttgacg cggtgcgggc gggcgacgtg attggggtgc gcgaagacgg aacggaagtg
    13321 cgcgcgccct acgacggctg cgtgctgttt ccgcaccccg aggcggatgt ggggcaggag
    13381 tggttctatc tggcggtgtt tgacgccgct gcgtgatttt tcaagcgatt ttgggcgctg
    13441 gcgcttgtgt atcaagcgcc agaagctatt gaattaatag taaacgcctc aggcgtcttc
    13501 cttgcccagc agcgagtcct tcagcttcca gtcggccggc ttggcgccga gccagatgcg
    13561 catcagggtt tgaaagaact ccggctcgcg aaacggctcg cccacggcgt gtcccttgat
    13621 ggtgagtacc gcgccggtgc ccggcaccca atcgacctga aagctctcgc ccgccttcag
    13681 cttcttgtgg tcgctgaaga cctggctcat gcgcatgatg ccgggcagca ggcgcaccat
    13741 gctgccgcgc tcggcgtttt cttccatgcc gcgctcgaac atcttgccca gctcggccga
    13801 ttcgatgtcg cgcagcatgg tgatggacaa gcgcttgggc gtgtcggtgg ccaccgcttc
    13861 ggtggccgat gcggtcttgg ccggcacgta cagccccgcc gcatacacct tgaacaccgc
    13921 cttgtagcgc acgccagcgc cgttcagctg cagcggcttg cccgccacgt tgacctgctg
    13981 cggaaactgc acgccggcca gctccacagc ccgcgcccct gcggacgaca tcagggcggc
    14041 gccacccagc atggctgcgg ccaccacgtg gcgcaaatgg gctcgcgccc gaccagcagt
    14101 tttccgagga ttcattccac gcaccaaaaa agaacgagcg ttcgataatg tgcgtgaact
    14161 gtcggttttt gtatcgggga aaacgagcgg cgcgtcggcg atccgtcagg cgacgacggc
    14221 cgtgtccttg atttcgtcgg tcaccacgtc gaagcgattg agctgctgcc agccaaacac
    14281 catggcgatc ggcacgtcgg tggcgtcgcg cccgcgcgcc atcacgatgc ggccgatgcg
    14341 gcgcgcgttg ttgcgcgcat cgaaggtgtg ccactggccg cccaggtaca cctccatcca
    14401 ggcggcaaag tccattggcg cggcggcggg cggcacgccg atgtcgccca ggtagccggt
    14461 gacgtagcgc gccggaatgt tcatgcagcg gcacaacgtg acggccaggt gcgcgtaatc
    14521 acggcacacg cccacgcctt cttgccagcc ctgcagcgcg gtgcggttgg cgcgcgcctg
    14581 catgtagtcg aagcggatgt ggtggtgcgt gtagtcgacg atggcctgca cgcgcgccca
    14641 gccctcgggc gtgttgccaa actggttcca ggccagttgc atcagctcgc tgtcgacctc
    14701 gcaatagcgg ctgggcagca gaaagcggaa cacttccagc ggcagctcgt gcagcgggtg
    14761 ctggcgcgcg tcgggcacca ccgggtcggg caggccgctg tcggccacca cggcgtggtt
    14821 ggtgaagcgc acgctggcca cgcccggtgg cacgtcaacg cgcgtgatgg tgttgtcgaa
    14881 gccgtcctgg taggtggaga tgggcagcgc cggctccacc tcgaattgct caggcgcctg
    14941 caaatcgtgc gcgcgcgaag ggtgcacgtg cagcgcgcac agcagcgtgg tgggggcgtt
    15001 gaccgccagt tcgatgtcgt agccaatctg aatcaacatg agggaaagcc tttcgccccg
    15061 aagatcacgg cggctggtgg gtgaatgaag gtgcgcgttg ccaagggcgg cgccactttc
    15121 attgggccac ccggcgcgcc gtgtgtatgc aagacaaatg ccacccggcg cgtcggtggc
    15181 gcgagcgctt gatcgccacg gccttttgct acactaagcc gcatgtgttc ttcccgcaat
    15241 gtcatttcac cgctgggcgc cgccgcccag ggggcgtggc cctggcgcaa gccagcacgc
    15301 tgacttgtga agcgcgcctg caacgggcgc acacggggca cgccagccgg cgcgccgcac
    15361 ctggaagacc gcagctcccc aaagcgccgc tcaaagccga aggaatttga agcgcccgag
    15421 ctgccccagc atgaaagctg aagatcgcca tgatcacgga actcgaattc aaaagcctgg
    15481 ccggccaagg ctacaaccgc atccccctga tggccgaggc gttcgccgat ttggagacgc
    15541 cactctcgct ttacctcaaa ctggcctctg gcgcaggcgg gtcgggcgcg gggcgctata
    15601 gttttttatt ggaatcggtg attggcggcg agcggtttgg ccgctacagc ttcatcggcc
    15661 tgccggcgcg caccatcctg cgcgcgagcg gatttggcgc caatgcgcgc accgaagtgg
    15721 tgaccgacgc aaaggtggtc gaaaccgccg aaggcaaccc gctcgacttc atcgccgact
    15781 accaaaagcg cttcaaggtc gcgctgcgcc ccggcctgcc gcgcttttgc ggcgggctgg
    15841 cgggctactt cggctacgac acggtccgcc acatcgagaa aaagctcgaa aaaacctgcc
    15901 cgcccgaccc gttggggctg cccgacatcc tgctgatgca cagcgaggaa gtcgcggtga
    15961 tcgacaacct gtcgggcaag ctgtacctga tcgtctatgc cgacccggcg cagcccgagg
    16021 cgtacagcaa cgccaagaag cgcctgaaaa cactgcgcga gcagttgcgt tattcggtca
    16081 gcgtgccgca ggtgaaagcc acgcagggct ttccggctga gcgtgaattt gccaaggccg
    16141 actacctgaa agccgtggag cgcgccaagg agctgatcg
//
LOCUS       DAOMVO010000021        15872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_752_length_15872_cov_14.124802, whole genome shotgun sequence.
ACCESSION   DAOMVO010000021 DAOMVO010000000
VERSION     DAOMVO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 15872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15872
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_752_length_15872_cov_14.124802"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..860)
                     /gene="prfB"
                     /locus_tag="PKV30_02245"
                     /pseudo
     CDS             complement(1..860)
                     /gene="prfB"
                     /locus_tag="PKV30_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123395.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            1041..2738
                     /locus_tag="PKV30_02250"
     CDS             1041..2738
                     /locus_tag="PKV30_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRL29073.1"
                     /translation="MGLGLQALLAFAPIALAGVLLVGMRMAARTAMPIVYVVTALIAL
                     LAWGVSFNRVAAASIEGLIITAQVLWIIFGALLLLNTLKYSGGIGTIRTGFAGLSGDR
                     RIQVILIAWLFGCFIEGASGFGTPAAVVGPLMVAVGFPALAAVTFGMIIQSTPVSFGA
                     VGTPLLVGVQGGLQRDAMTQALQAQGHTWADFFHVVVSQVAIIHAICGTLIPLFLVMF
                     MTRFFGKNRSWSEGLAAAPFALFAALAFTIPYVLAAVVLGPEFPSIIGGMLGLAVAAL
                     AARAGFLVPRKSWDFPPAAEWPKEWVGSLHMELGANERRMPLLMAWLPYVLLALFLVM
                     TRTIEPLKQAVTSVQWSVRGILGETGINGGFQPLYLPGGILLLVCLITVLLHRMKWSD
                     FGKAAGESARTLVGAGFVLVFTVPMVRIMIQSGVNAADLASMPIAMARWVADSVGGAY
                     PLFAPSVGALGAFLAGSNTVSNLMLSQFQYSVAQALGVSGATLVAAQSVGAAAGNMIA
                     IHNVVAASATVGLLGREGVTLRRTIIPTVYYVALAGVLTFVALRVLGVTDPMMGVKF"
     gene            complement(2898..3830)
                     /locus_tag="PKV30_02255"
     CDS             complement(2898..3830)
                     /locus_tag="PKV30_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29074.1"
                     /translation="MYIAQRPSRSNSVAIRQQQVHVRLWGERASEQPPLVLLHGWMDV
                     AASYQFMVDALSADFFAGRFILAPDWRGFGRSTVPACDHYTFADYLGDLEGLLDHFAG
                     DRPVDLVGHSMGGNIAMMYAGARPSRVRRLVNLEGFGMPATLPAQAPTRYAKWLDEIK
                     SLHRGEMALRTYDDESGVAARLMKTNPRLSPDKAQWLAREWSEPRTDADGRTRWHILG
                     DAAHRVINPQLFRVDEMLALYAHIAAPTLMVEARDDSLAGWWQGRFTRDEFHERLKAV
                     PTLRREVIQDAGHMLHHDQPETLAALVESFLGTD"
     assembly_gap    4395..4494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4497..5741
                     /locus_tag="PKV30_02260"
                     /pseudo
     CDS             4497..5741
                     /locus_tag="PKV30_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857433.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            5802..7541
                     /locus_tag="PKV30_02265"
     CDS             5802..7541
                     /locus_tag="PKV30_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRL29075.1"
                     /translation="MSALAAAPAATPPAPARARIRGAGWGVWLAFGLIAAFLLAFLIV
                     PIGLVIYTAFVDESNRLTLGHFANFFGQTVFVESFLNSLWVSLASVALATLIAVPLAY
                     LTVRFEFRGALLIQTLGVLPLIMPPFVGAVAMQLIFGRNGSVNLLLNDWFAFTVPIMD
                     GLIGVTFVESIHYFPFILLNLVASMRNIDGAMEESALNLGARGWRLFRRVIFPLAMPG
                     YLAGATLVFVKVFDDLGTPLVMGVTNMLAPQAYLRITSVGIDDPQGYVISVIMIAFSI
                     LALGLAARVMKGRDYSTLQKGGNSLQRRRLSGWESVLAYGWIILVLLITLAPHFGILL
                     MSFAKVWSFSVLPDAYTLEHYATVFTDARGMMANTLLYCLLAAGLDVVIGTAIAYLIL
                     RTNLPGRRWLDYMASAALAIPGLVLAIGYLRLFKGVNLPFTDTPVVSTWVLIMLAYAV
                     RRLPYALRSCMAALQQVHVSLEEAAQSLGATRMSTIRRVMVPLMMGGMLAGFVTSFIT
                     AAVELSATILLASAQSQAPMSYGIYLYMQSIAGRGPGAALAVLAVVVVAVGTYLSHRF
                     VERSRATVATARV"
     gene            7555..8649
                     /locus_tag="PKV30_02270"
     CDS             7555..8649
                     /locus_tag="PKV30_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29076.1"
                     /translation="MQALNKVSLEARHISLSYGATPVLQGVNLRVEPGEFFALLGPSG
                     SGKSTLLRLIAGFNQHQSGELLVDGKDITGVPPHARNVGMVFQNYALWPHMTVWDNVA
                     FGLVERRESRDAIRRKVGDVLALVGLSDYAERRPAQLSGGQQQRVALARTVVIEPKLL
                     LLDEPLSNLDKQLRVQMREELKHLQRKLGLTTLFVTHDQEEAMTTADRMAVLDKGVLQ
                     QVGSASGLYDYPANRFVASFVGTANVLEGQVTSVGGDTIGFHANGLGDIRLPRPPEAP
                     AVGAAALAFRPHQVSIRVRDEQTDASRLWVDGVVESAEFLGEFSRYRVRAGEVALITD
                     QPHYAGVPLFPTGAQVRLGIEPTQLRFLQD"
     gene            8682..9569
                     /locus_tag="PKV30_02275"
     CDS             8682..9569
                     /locus_tag="PKV30_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL29077.1"
                     /translation="MEIYQLRAFATAARLGNLTRTAEALHLTQPAITAQIKALEEQLG
                     VALFERRPGGITLTRAGELLLQDAEQILTTAGHLQGRARELQGEVTGQLVLGTLGDPE
                     SLRLGTLRVVLSEKLPLLEIKTRQGAAEDVREQVAAGLLRGGFYIGMQLPGEVGGLVL
                     QTIHYRIVAPWRDRERVLHAGWRELAAMPWVGASPRHHVQVLLHGLFARQGLAPHQTI
                     ESDEVALPFSLARAGVGLALVREELALQGSERQEVVIWPHARVSAQLAFIYSHAAEHD
                     PALVATLSALRGVWGLAGR"
     gene            9724..10782
                     /locus_tag="PKV30_02280"
     CDS             9724..10782
                     /locus_tag="PKV30_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRL29078.1"
                     /translation="MQTVSLGTSDLRVTPICLGTMTFGEQVNEADSHAILDRAIERGV
                     NFIDTAEMYPVPPSAPTYSRTETFIGNWLSRRPGMRQKIVLATKVAGPSRGMPWVREG
                     QGMTPADIEASCNASLKRLQTDVIDLYQIHWPERHVPAFGNLYYDPAQEKSETPIHDQ
                     LEALAKLVKAGKVRAIGLSNETPYGVHEFVRLAEEHGLPRIASTQNPYCLISRSYDNG
                     LDETCHRLNVALLAYSPLGFGLLTGKYDVTGIHSSQAPRGRMSLFDRMKNQRWGRESS
                     IAAAKRYNDLAREHGLTPTQLALAFCYGSWRVASTIIGVTTLAQLDEDIDAWNVQLSP
                     ELLAACDAIRLEMRDPAN"
     gene            10899..11168
                     /locus_tag="PKV30_02285"
     CDS             10899..11168
                     /locus_tag="PKV30_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29079.1"
                     /translation="MPARHNDLPLNEKSLNAVVDAMTVVTLCMTQILTHEQRERFGKD
                     LVTMADVAGRKGKLELTSILLDVRAAVKKHDDELEAARAENDAAA"
     gene            11176..11667
                     /locus_tag="PKV30_02290"
     CDS             11176..11667
                     /locus_tag="PKV30_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HRL29080.1"
                     /translation="MAKAAHVSETPATALLRQHGVAFTEHPYEYVEHGGATHSANVLG
                     FDPFAVVKTLVMQDQDAKPLIVLMHGNRKVSTKNLARQIGAKSVEPCAPEVANRHSGF
                     LVGGTSPFGTRRAMPVYIEQTILKLPRMAINGGRRGYLIGIDPQVCVQLLGAKPVQCA
                     IDL"
     gene            11764..13881
                     /locus_tag="PKV30_02295"
     CDS             11764..13881
                     /locus_tag="PKV30_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HRL29081.1"
                     /translation="MQSGKSSITQLFGAPVQYVIPVFQRGYVWTLTKQVGPLWADIED
                     RAKKLREHDAQAAQVGQQPLRPVQKHFLGSVVLTPVPGAFGRVPSFEVIDGQQRTTTL
                     LLLLLAFWHAARQLPESPVADMLKSVLHNQGTFAQDSDGFKVWPTQAGRAEMRALLQA
                     ADIASIQGAYPARDGRKRLERPLMVQSFLYLHHACLAWLRGVELSDALAAQDERSLSD
                     ALIYAVAHDNDVAAIESELPLLPQRAQSLFMALQSLVQLMTLTLEAEDDPQVIFETLN
                     ARGEPLLASDLVRNFVFLEAARRGLDVPALYQRHWKGFDEQADAKGAATANRYWREQE
                     RQGRLTHPRIDLFFYHYTVLRRGEETKVAHVFQSFKDWWRSAPERDIGAELARLVQSS
                     EHFRDLVSPDGSDDVAEFGRLMRALDVFSFAPAYLALRERLPNGSPELKQALGDLASY
                     LVRRAVCGLTTKAYNRLALRLMTEIQAAEVPAAALRRYLRGLDGDTQVWPDDRRFGDQ
                     WLARPVYLELKPARVSALLRLLERASRTTYQAGIGVPLASTLSVEHVLPQNWEGSGYY
                     PLPTHAPIPMEADAPRELTTDEVQQARARRERLLHTFGNLTLLTQPMNSALSNGPYAD
                     QYDQNGTRTAEGKRSGLRDGLLSLNSWFQRPEITEWNDARIEERGAQLLEQAVACWPH
                     PPAEPAKISGQQVAAGVQENPTP"
     gene            13878..14501
                     /gene="plsY"
                     /locus_tag="PKV30_02300"
     CDS             13878..14501
                     /gene="plsY"
                     /locus_tag="PKV30_02300"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRL29082.1"
                     /translation="MIFSLLATLIAYLVGSLSFAVIVSRAMGLSDPRTYGSGNPGATN
                     VLRSGSKPAAIVTLLLDAAKGWLPVMLVKWFGPAWDLSEGTQALAGLAAFLGHLYPVF
                     FAFKGGKGVATAVGVLLAFQPLLALATLLTFAIIVFFTRYVSLASMVAAVFAPAYYLI
                     GDGVAWTASGAKTLALIAMGLLLIWRHRENIQRLLAGTESKLGQKKT"
     gene            14498..14851
                     /locus_tag="PKV30_02305"
     CDS             14498..14851
                     /locus_tag="PKV30_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL29083.1"
                     /translation="MSTIARHGVAARYSEASVFNGVVYLAGMVPEGAATDIRSQTQDV
                     LAQIDAHLAAQGSDKTRLLRVQIYLSDIADIGAMNEVWDAWVAPGHAPPRATVQAALA
                     DAAWKIEVVATAAQR"
     gene            complement(15042..15653)
                     /locus_tag="PKV30_02310"
     CDS             complement(15042..15653)
                     /locus_tag="PKV30_02310"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRL29084.1"
                     /translation="MLATFTLGASAQTVALQGMLGSKALLIVGGGAPRAVAPGETHQG
                     VKVISTSGDQAVVLVDGRRVTLRVGEAPASVGGAAPSGGDRIALTADGRGHFIAQGSI
                     NNRPVQFMVDTGASVVAIGQGEADRLGLNYKSGRQVMMNTANGAAPGWLFKINTLRVG
                     DVVAYEVDTVVTPAAMPAILLGNSFLNRFNMRRDGDQMMLIKR"
     gene            complement(15748..>15872)
                     /locus_tag="PKV30_02315"
     CDS             complement(15748..>15872)
                     /locus_tag="PKV30_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HRL29085.1"
                     /translation="AIQGDSVRVTGAKRDDLQAVMALLRKDMADLPLSFDNFRD"
BASE COUNT         2609 a         5117 c         5230 g         2816 t
ORIGIN      
        1 cgcggttgct gtgctggctg cggctgtcct ggcactgcac cacgatgccg gtggggatgt
       61 gcgtcaggcg caccgccgaa tcggtcttgt tgatgtgctg gccgccggcg ccgctggcgc
      121 ggtaggtgtc ggtgcgcacg tcggccgggt tgatgtcgat ctcgatcgag tcgtcgacct
      181 cggggtagac gaagatcgac gcgaacgagg tgtggcgccc gcccgatgaa tcgaagggcg
      241 acttgcgcac cagccggtgc acgccggttt cagtgcgcag caagccgtag gcgtactcgc
      301 cttcgacctt gatggtggcg cccttgatgc ccgcggtttc gccgggcgtc tcgtcctcga
      361 tgtcggtctt gaagcccttg cgctcggcgt atttcaggta ctggcgcagc agcatgctgg
      421 cccagtcctg cgcctcggtg ccgccggcgc cggcctggat gtcgatgaag gcgttgttcg
      481 ggtcggccgg ctggttgaac atgcggcgga actcgaggtc ttccaccgtc ttttcgaggg
      541 ccgatacgtc gtcggcgatc gacagcaggg cgctttcgtc ctcttcttcg cggctcatct
      601 cgaacagctc ggcgttgtcg gacagccctt gcgtcagctc atcgagcgtg acgaccacgc
      661 cgtcgagcgc cttcttttcc ttgcccagtt cctgcgcttt cttggggtcg ttccagaccg
      721 ccgggtcttc cagcgcggcg ttgacggttc tcaggcgttc ggacttttca gcgtagtcaa
      781 agataccccc gtagcgcgtc ggtgcgcgcg cgaaggtcat cgatgcggtt gccaatctgg
      841 ttgatgcgtt ctgcgtccat ggcgagggtc tttcttgaag gcgtggcagc accgagtgct
      901 gacgaaaagc gcaaattttc gcatggcccc acgctgcgca cggggtttgg ccattgtgcg
      961 catttgcggc atgattgtgc agcgccgctc atcgcaagag cacgctacac ggacaagagc
     1021 catcacccga ggagacaagc atgggactag gactgcaggc gctgctagcg ttcgcgccga
     1081 tcgcactggc aggcgtattg ctggtgggca tgcggatggc ggccaggacg gcgatgccga
     1141 tcgtctatgt cgttacggcg ctgattgcgc tgctggcctg gggggtgagc ttcaaccgcg
     1201 tggctgcggc atcgatcgaa gggctcatca tcacggcgca ggtgctgtgg atcatcttcg
     1261 gcgcgctgct gctgctcaac acgctgaaat attccggcgg catcggcacc attcgcaccg
     1321 gctttgcggg gctgtcgggc gaccggcgca tccaggtcat cctgatcgcc tggctgttcg
     1381 gctgcttcat cgagggcgct tcgggcttcg gcacgccggc agcggtggtg gggccgctga
     1441 tggtggcggt gggctttccg gcgctggcgg cggtgacctt cggcatgatc atccagtcga
     1501 cgccggtgtc gtttggcgcc gtgggcacgc ctttgctggt gggcgtgcag ggcgggctgc
     1561 agcgcgacgc gatgacgcag gcgctgcagg cgcagggcca cacatgggca gacttcttcc
     1621 acgtagtggt gtcgcaggtg gccatcatcc acgccatctg cggcacgctg atcccgctgt
     1681 ttctggtcat gttcatgacg cgcttcttcg gcaagaaccg ctcctggagc gagggcctgg
     1741 cggctgcgcc gtttgcgctg ttcgcggcgc ttgcgttcac catcccctat gtgctggcgg
     1801 ccgtggttct ggggcctgag tttccgtcca tcatcggcgg catgctgggc cttgccgtgg
     1861 cggcgctggc ggcgcgcgcc ggctttctgg tgccgcgcaa gtcatgggac tttccgcccg
     1921 ctgccgaatg gcccaaggag tgggtcggta gcctgcacat ggaactgggc gccaacgaaa
     1981 ggcgcatgcc gctgttgatg gcctggctgc cctatgtgct gctggcgcta ttcctggtga
     2041 tgacgcgcac aatcgagccg ctcaagcagg cggtgaccag cgtgcagtgg tcggtgcgcg
     2101 gcattctggg cgagacgggc atcaacggcg gctttcagcc gctataccta ccgggcggca
     2161 tcctgctgtt ggtatgcctg atcaccgtgt tactgcaccg catgaagtgg agtgacttcg
     2221 gcaaggcagc cggtgaatcc gcgcgcacgc tggtgggcgc gggcttcgtg ctggtgttca
     2281 ccgtgccgat ggtgcgcatc atgatccagt cgggcgtgaa tgcagccgac ctggccagca
     2341 tgccgattgc gatggcgcgc tgggtggccg actcggtggg cggtgcctat ccgctgtttg
     2401 cgccctcggt gggggcgctc ggggcgttcc ttgcgggcag caacacggtg tcgaacctga
     2461 tgctgagcca gttccagtac agcgtggcgc aggcgctggg cgtgagcggg gccacgctgg
     2521 tggcagcgca atcggtgggc gcggcggcgg gcaacatgat cgccatccac aacgtggtgg
     2581 ccgcgtcggc cacggtgggg ctgctggggc gcgaaggcgt caccctgcgg cgaacgatca
     2641 tcccgaccgt gtactacgtc gccctggccg gcgtgctgac cttcgtggcg ctgcgcgtgc
     2701 tgggcgtgac ggacccgatg atgggcgtga agttctaggc gcgcggccgg caccgatggc
     2761 gccgaataca aagcgtttcg gccctccggc gcttgatgga caagcgcgag cagctatgaa
     2821 aaacatagta tctgatcgag gtcgatgacc ggtcatcggg gcacggacca gcgcggtcac
     2881 gacattggca gcgcctgtca gtcggtgccg aggaaggatt ccaccagcgc cgccagcgtc
     2941 tcgggctggt cgtggtgcag catgtggccg gcatcctgga tcacctcgcg ccgcagcgtg
     3001 ggcacggcct tcaggcgctc gtggaactca tcgcgggtga agcggccctg ccaccagcct
     3061 gccagactgt cgtctctggc ctcgaccatc agcgtgggcg cggcgatgtg cgcataaagc
     3121 gccagcattt cgtcgacgcg aaagagttgc ggattgatca cccggtgcgc cgcgtcgccc
     3181 aggatgtgcc agcgcgtgcg gccgtcggca tcggtgcgcg gctccgacca ttcgcgcgcc
     3241 agccactgcg ccttgtcggg cgacaggcgc gggttggtct tcatcaggcg cgccgccaca
     3301 ccgctctcgt cgtcgtaggt gcgcagcgcc atctcgccgc ggtgcagtga cttgatttcg
     3361 tccagccact tggcatagcg cgtgggcgcc tgcgcgggca gggtggcggg catgccgaag
     3421 ccttcgaggt tgaccagacg ccgcacccgt gaaggccgag cgcccgcata catcatggcg
     3481 atgttgccgc ccatgctgtg cccgaccaga tcgaccgggc ggtcgccggc aaagtgatcc
     3541 agcaagccct ccagatcgcc cagatagtcg gcaaaggtgt agtggtcgca cgccggcacg
     3601 gtggagcggc cgaagccgcg ccaatcaggc gccaggatga aacggccggc aaaaaaatcg
     3661 gccgacagcg cgtcgaccat gaactgatag ctggccgcca cgtccatcca gccgtgcagc
     3721 agcaccagcg gcggctgctc gctggcccgc tcgccccata ggcgcacgtg gacttgctgc
     3781 tggcgaatcg caacggagtt gctgcgcgag gggcgttggg cgatgtacat cgggcaaatc
     3841 ttagaggagc gcagccagcg tatcggcacc gcctggcgcc gatgtgactg cgcgctcgat
     3901 ttgcgtgaag gcagccggcc cgagctgcag tggccgtgca aggcggcggc tcggctatgc
     3961 cagtgccttc gcttttgcgt tcaagctact aaaaacatag ctaaaagcgc ttgatggaca
     4021 agcgccagag ccaaattcga tccttcaaaa gtctggggtc gggtgcggtg cctcgcacct
     4081 gatcgcacac gccgtctggg ctgcggcaca tggcggcgag gacaacctgc gcgggttgtg
     4141 cccgtgcttt ccggcctgac gggcggcatc acaaaccctg attggccacc tgcggtgcat
     4201 cgggctggag tgcgatagcc gtcacggctt cgtcacgtga ctgtcgcatt caaaggagac
     4261 ctcatcatgc acctttgccg ttccgcggct gccctggccg tactggccgc atcgacatgg
     4321 gtgcatgcgg gcaccgtcac cgtgctcacg tctttcccca aggagctgac gaccgcctat
     4381 cagaaagctt tcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgtgc
     4501 tcacgtcttt ccccaaggag ctgacgaccg cctatcagaa agctttcgag gccaagaacc
     4561 ccggcatcaa ggtcgagatc ctgaacaaga acaccgtcgc cggcatcgcg tatgtgcgcg
     4621 agctgccggc cggccagcgg cccgacatca tgtgggcctc ggccccggat gccttcgagg
     4681 tagtggcgcg caacaagctg ctgcaagccg cgcccgacac caggaacgcc gccgcgcccg
     4741 caaagatcgg caactatccg ctgaacgacc ccgatggcct gtactacggc caggcgctgg
     4801 ccggccacgg catcatgtgg aacacgcgct acctggcggc caacaagctg ccggcgctca
     4861 agtagtggaa ggatctgacc cggccggagt agttcggcca tgtcgccatc tcgtcgccct
     4921 cgcgctcggg caccacgcag ctcacggtgg agacgatcct gcagggcgaa ggttgggaaa
     4981 agggctgcaa ccagttgctg gaaatgatgg gcaacgccgc cgccgtcacc gaccgcagct
     5041 tcgcggtgcc ggacggcgtc cacaacggcc agtacggcgt cggcatcgtg atcgacttct
     5101 tcggtctggc cggcaagtac tcgggctttc cggtggactt cgtgtagccg agcatgacct
     5161 cggtggtgcc ggccaacatc gcgctgatcg ccggtggcaa gaaccccgac gaggccaaga
     5221 agttcatggc cttcagcatg tcgaccgaag gccagcaact gctgttcgac cccaagatca
     5281 gtcgcctgcc gatcctgccc tacagccagt tgaaggcgcc ggccggctac cccgatccgc
     5341 aggaggtcgc caagcgcgcc aaggtgcagt tcgacgccaa cctgtccgag gcgcgctacc
     5401 aggtcgtcac cagcctgttc gaccagatgg tgaccttccg cctgaaagag ttgcaaggcg
     5461 cgacaaaggc cattcaggac gccgaaaagg cgctcaaggc caagcccaac gcgcgcggca
     5521 gcgatctggt caagcaggcg cgggcgctgg cctatacgcc gctcgtgggc gccgagaacg
     5581 tgaagaacga gcagtttctg gaactgttcc gcaagagccg gcgcgacgtg gaagtctcca
     5641 agcagctctc agggcaagag cagttgtggg ccaacaaggc gcgtgagaac tacgcgcgcg
     5701 cgcagcaact cgccactgag gcgcgtggcc tggccaagtg agcggcaccg ctgggccatg
     5761 tgcccggtgg ttcgaccccc tacggtcttt ttagctgacc catgtccgca ctcgcggcgg
     5821 caccggccgc cacgcctcct gcgcccgcgc gtgcccgcat ccgcggcgcc ggctggggcg
     5881 tctggctggc cttcgggctg atcgccgcct ttctgctggc cttcctgatc gtgccgatcg
     5941 ggctggtcat ctacaccgcg tttgtcgacg agagcaaccg gctcacgctg ggccactttg
     6001 ccaacttctt cggccagacg gtgtttgtcg agtcgtttct caacagtctg tgggtgtcgc
     6061 tggccagcgt ggcgttggcg acgctgatcg ccgtgccgct ggcctacctg acggtgcgtt
     6121 tcgagttccg cggcgcgctg ttgatccaga cgctgggcgt gctgccgctg atcatgccgc
     6181 cctttgtcgg tgcggtggcc atgcagctca tctttggccg caacggctcg gtcaacctgc
     6241 tgctgaacga ctggttcgcc ttcacggtgc cgatcatgga tgggttgatt ggcgtgacct
     6301 ttgtcgagag catccactac ttcccgttca tcctgctcaa cctggtcgcc agcatgcgca
     6361 acatcgacgg cgccatggaa gagtcggcgc tgaacctggg cgcgcgcggc tggcggctgt
     6421 ttcggcgcgt gatatttccg ctcgccatgc cgggctacct ggcaggcgcc acgctggtgt
     6481 tcgtgaaggt gttcgacgac ctcggcacgc cgctggtgat gggcgtgacc aacatgctgg
     6541 cgccgcaggc ctacctgcgc atcacctcgg tcggcatcga cgatccgcag ggctacgtca
     6601 tcagcgtgat catgatcgcg ttctcgatcc tggcgctggg gctggccgcg cgggtgatga
     6661 aggggcgcga ctattccacg ctgcaaaagg gcggcaactc gctgcagcgg cggcgcctgt
     6721 cgggctggga atcggtgctg gcctacggct ggatcatcct cgtgctgctc atcacgctgg
     6781 cgccgcactt cggcattttg ctgatgtcgt ttgccaaggt gtggagcttc tcggtgctgc
     6841 ccgacgccta cacgctggag cactacgcca ccgtgttcac cgacgcgcgc ggcatgatgg
     6901 ccaacacgct gctgtactgc ctgctggcgg ccgggctgga cgtggtgatc ggcaccgcca
     6961 tcgcctacct gatcctgcgc accaacctgc cgggccgccg ctggctcgat tacatggcct
     7021 cggccgcgct ggcgattccc ggcctggtgc tggccatcgg ctacctgcgc ctcttcaaag
     7081 gcgtcaacct gccgttcacg gataccccgg tggtgagcac ctgggtgctg atcatgctgg
     7141 cctacgcggt gcggcgcctg ccctacgcgc tgcgctcgtg catggcggcc ttgcagcagg
     7201 tgcatgtgtc gctggaagag gctgcgcaaa gcctgggcgc cacgcgcatg agcaccatcc
     7261 gccgcgtgat ggtgccgctg atgatgggcg gcatgctggc cggctttgtc accagcttca
     7321 tcacggcggc ggtcgagctg tcggccacca tcctgctggc gtcggcgcaa agccaggcgc
     7381 cgatgagcta cggcatctac ctctacatgc agagcatcgc cggccgcggc ccgggcgcag
     7441 cgctggccgt gctggcggtg gtggtggtgg ccgtgggcac gtacctgtcg caccgcttcg
     7501 tcgagcgttc gcgcgctacc gtggccacag cgcgcgtgta gagggaaaca agtcatgcaa
     7561 gcactcaaca aagtctcgct cgaagcgcgc cacatcagcc tgagctacgg cgccaccccg
     7621 gtgttgcagg gcgttaacct gcgcgtcgaa ccgggtgaat tttttgcgct gctgggcccc
     7681 tcgggctcag gcaaatccac gctgctgcgg ctgattgccg gcttcaacca gcaccagagc
     7741 ggtgagttgc tggtcgacgg caaggacatc accggcgtgc cgccgcacgc gcgcaacgtc
     7801 ggcatggtgt ttcagaacta cgcgctgtgg ccgcacatga cggtgtggga caacgtggcc
     7861 ttcggcctgg tcgagcggcg cgagtcgcgc gacgccatcc gccgcaaggt gggcgacgtg
     7921 ctggcactgg tgggcctgtc ggactacgcc gagcgccggc cggcgcaatt gtcgggcggg
     7981 cagcagcagc gcgtggcact ggcgcgcacc gtggtgatcg agcccaagct gctgctgctg
     8041 gacgaaccgc tgtccaacct ggacaagcag ttgcgtgtgc agatgcgcga agagctcaag
     8101 cacctgcagc gcaagctggg gctgaccacg ctgttcgtca cccacgacca ggaagaagcc
     8161 atgaccaccg ccgaccgcat ggcggtgctg gacaagggcg tgctgcagca ggtcggctcg
     8221 gcctcgggcc tgtacgacta cccggccaac cgcttcgtcg ccagtttcgt cggcaccgcc
     8281 aacgtgctcg aagggcaggt cacgtctgtt ggcggcgaca cgatcggctt tcacgccaac
     8341 ggcctgggcg acatccgcct gccgcgcccg cccgaggcgc cggccgtcgg tgcggcggca
     8401 ctggcgtttc gcccgcacca agtcagcatc cgcgtgcgcg acgaacagac cgacgcctcg
     8461 cgcctgtggg tcgacggcgt ggtcgaaagc gccgagtttc tgggcgagtt ttcgcgctac
     8521 cgggtgcggg caggcgaggt ggcgctcatc accgaccagc cgcactacgc cggcgtgccg
     8581 ttgtttccca ccggcgcgca ggtgcgcctg ggcatcgagc caacgcagtt gcgctttctg
     8641 caagactgaa ctgaagcggg cgcatcggcg atcatcgcgg catggagatt taccagctgc
     8701 gcgcctttgc caccgccgcc cggctgggca acctgacgcg caccgccgag gcgctgcacc
     8761 tgacgcagcc ggccatcacg gcgcagatca aggcgctgga agagcaactt ggcgtggcgc
     8821 tgttcgagcg ccggcccggc ggcatcacgc tgacacgcgc gggcgagttg ctgctgcagg
     8881 atgcggagca aattttgacc accgccggcc acctgcaggg ccgcgcgcgt gagctgcaag
     8941 gcgaagtcac tggtcaactc gtgctgggca cgctgggcga ccctgaatcg ctgcgcttgg
     9001 gcacgctgcg tgtcgtgctg tcagaaaagc tgccgctgct ggaaatcaag acgcgccagg
     9061 gcgctgccga agacgtgcgc gaacaggtcg cggccggcct gctgcgcggc ggtttctaca
     9121 tcggcatgca gttgccgggc gaagtgggcg gtttggtgct gcagaccatc cattaccgca
     9181 tcgtcgcgcc ctggcgcgac cgcgagcgcg tgctgcacgc cggctggcgt gaactggcgg
     9241 ccatgccctg ggtcggcgcc tcgccgcgcc accacgtgca ggtgctgctg cacggcctgt
     9301 tcgcgcgcca gggcctggcg ccgcaccaga ccatcgaatc ggacgaagtg gcgctgccct
     9361 tcagcctggc gcgcgccggc gtcggcttgg cgctggtgcg tgaggagctg gcgctgcagg
     9421 gcagcgagcg gcaagaggtg gtgatctggc cgcacgcgcg cgtgtcggcg caactggctt
     9481 tcatctattc gcacgcggcc gagcacgacc cggcgctggt ggccacgctg tcggcgctgc
     9541 gcggggtgtg ggggttggcc gggcgatgat gctaatacta ttaattcagt agctgctcgc
     9601 gcttttcaga caagcgcgag agcgtgtttt tctataaatc tgatgggcat tgtgtcgacg
     9661 agcggcccga tgcggcgccg ttacgtaaac tgcagcttgt tcattgccac gcccccgctt
     9721 tttatgcaaa ccgtttccct cggcaccagc gacctgcgcg tcacccccat ctgcctcggc
     9781 accatgactt ttggcgagca ggtgaacgaa gccgattcgc acgccatcct cgaccgcgcc
     9841 atcgagcgcg gcgtcaactt catcgacacg gccgagatgt accccgtgcc gcccagcgcg
     9901 cccacctaca gccgcaccga aaccttcatc ggcaactggc ttagccgccg ccccggcatg
     9961 cgccagaaga tcgtgctggc caccaaggtc gccggtccct cgcgcggcat gccctgggtg
    10021 cgcgaagggc agggcatgac gccggccgac atcgaagcca gctgcaacgc cagcctgaag
    10081 cgcctgcaga ccgacgtcat cgacctgtac cagatccact ggcccgagcg ccacgtgccg
    10141 gcgtttggca acctgtacta cgacccggcg caggaaaagt ccgaaacgcc gattcacgac
    10201 cagcttgaag cgctcgccaa gctggtgaaa gcgggcaagg tgcgcgccat tggcctgtcg
    10261 aacgaaacgc cttacggcgt gcacgaattc gtgcgcctgg ccgaagagca cgggctgccg
    10321 cgcatcgcca gcacgcaaaa cccgtattgc ctgatcagcc gcagctacga caacggcctg
    10381 gacgaaacct gccaccgcct gaacgtggcg ctgctggcct attcgccgct cggcttcggc
    10441 ctgctgacgg gcaagtacga cgtcaccggc atccactctt cgcaagcgcc acgcggccgc
    10501 atgagcctgt tcgaccgcat gaaaaaccag cgttggggcc gcgaatcgtc catcgccgct
    10561 gccaagcgct acaacgacct ggcgcgcgaa cacggcctga cgccgacgca gctggcgctg
    10621 gcgttttgct atggctcatg gcgcgtggcc agcaccatca tcggcgtgac cacgctggcg
    10681 cagctcgatg aagacattga tgcctggaac gtgcaactct cgcccgaact gctggccgcg
    10741 tgcgatgcca tccggctgga gatgcgcgac cccgccaact gacccgcgcg gtgtggacaa
    10801 ggccaatgtg cgcccgatgc gcatgaaatg cgtgagaaat caccttctcg cgccgagcgg
    10861 ctggctttaa gattttcccc attcactgcg agaccaccat gcccgcccgc cacaacgacc
    10921 tgcccctgaa cgaaaaatca ctcaacgccg tcgtcgacgc gatgacggtg gtcacgctct
    10981 gcatgacgca aatcctcacg cacgagcagc gcgagcgctt cggcaaggac ctggtcacca
    11041 tggccgacgt cgccggccgc aagggcaagc tggagctgac ctcgatcctg ctcgacgtgc
    11101 gcgcggcggt gaagaagcac gacgacgagc tcgaagccgc gcgcgccgag aacgacgccg
    11161 ccgcctgaac caccgatggc caaggccgcg cacgtcagcg aaacgcccgc caccgcgctg
    11221 ctgcgccagc acggcgtggc cttcaccgag cacccttacg agtacgtcga acacggcggc
    11281 gccacgcaca gcgccaatgt gctgggcttc gatccgtttg ccgtcgtcaa gacgctggtc
    11341 atgcaggacc aggacgccaa acccctcatc gttttgatgc acggcaaccg caaagtcagc
    11401 accaagaacc tggcgcgcca gatcggcgcc aagtcggtcg agccgtgcgc gcccgaagtg
    11461 gccaaccggc acagcggctt tctggtcggc ggcacctcgc ctttcggcac gcggcgcgcc
    11521 atgccggtgt acatcgagca gaccatcctc aagctgccgc gcatggccat caacggcggc
    11581 cggcgcggct atctcatcgg catcgatccg caggtgtgcg tgcaactgct gggcgccaag
    11641 ccggtgcagt gcgccatcga tctgtgagtt gctcgtggcc cgccgccatg acgcgacgag
    11701 cttgcagatt ggccgcacca taatttgctg cttcgctgca ccccaatctt ttagaaaaca
    11761 acgatgcaat cgggtaagtc ctccatcacc caactgttcg gcgcacccgt gcagtacgtc
    11821 atccctgtgt ttcaacgggg ctatgtttgg acgttgacca aacaggtcgg gccactgtgg
    11881 gccgatatcg aggatcgcgc gaagaagctg cgtgagcacg atgcccaagc cgcgcaagtc
    11941 ggtcagcaac cactcaggcc ggtacaaaaa cacttcctcg gttcggtggt gctgacgcct
    12001 gtgccgggcg cgtttgggcg cgtgccgtcg tttgaagtca tcgacggcca gcagcggacc
    12061 accacgctgc ttctgctgct gttggcgttt tggcatgctg cgcgccagtt gcccgaatca
    12121 ccggtcgccg acatgctcaa gagcgtgctg cacaaccagg gcacgtttgc gcaggacagc
    12181 gacggtttca aggtctggcc cacgcaagcg gggcgcgccg agatgcgcgc gttgctgcag
    12241 gctgctgata tcgcgtcgat acaaggggcg tatcctgcgc gcgatggccg caagcgactg
    12301 gagcggccgt tgatggtgca gagcttttta tatttgcacc atgcatgcct ggcttggctg
    12361 aggggcgtgg agctaagcga tgccttagca gcgcaggacg agcgcagcct gtcggatgcc
    12421 ttgatctacg ccgttgcgca tgacaatgac gttgccgcga tcgagtccga actgccgttg
    12481 ttgccgcaac gtgcccaaag tctgttcatg gccttgcagt cgctggtcca gctcatgacg
    12541 ttgacgctgg aagccgagga cgatccgcag gtcatcttcg agacgctcaa cgcgcgcggc
    12601 gagcccttgc tggcgtctga tttggtgcgc aacttcgtct ttctggaggc tgcgcggcgc
    12661 ggcctggatg tgccggcttt gtatcaaagg cactggaaag gtttcgacga gcaggccgat
    12721 gccaaaggcg ccgcgacggc caaccgctac tggcgcgagc aggagcgcca ggggcgcctg
    12781 acgcatccgc gcatagacct cttcttctat cactacaccg ttttgcggcg cggcgaggaa
    12841 accaaggttg cgcacgtatt ccagagcttc aaagactggt ggcgcagcgc acctgagcgc
    12901 gacatcggcg cggaactggc gcgattggtg caaagcagtg aacatttcag ggacttagtt
    12961 tcacctgacg gcagcgacga tgtagccgaa ttcgggcgct tgatgcgtgc gctggacgta
    13021 ttctcgttcg cgcccgccta cctggcgctg cgtgaacgcc tgcccaacgg cagcccggag
    13081 ttgaagcaag ccctgggcga tctggcttca tacctggtgc ggcgtgccgt gtgcggactc
    13141 acgaccaagg cctacaaccg acttgcgctg cgcctgatga ccgaaatcca ggcggccgaa
    13201 gtgcctgccg cagcactgcg tcgatatctg cggggattgg atggagacac gcaggtctgg
    13261 cccgacgacc ggcgttttgg tgaccaatgg cttgcccggc ccgtgtatct tgaactcaaa
    13321 ccggcacggg tgtcggccct cctgcggctt ctggaacggg cttcgcgcac cacttaccag
    13381 gcgggtatcg gtgtacccct agcttccacc ctgagtgtcg agcacgtatt gccgcagaac
    13441 tgggaaggct cgggttatta cccgctgccg acgcacgcgc cgatcccgat ggaagctgat
    13501 gcgccgcgcg agctgacgac cgatgaagtg caacaggcgc gcgcccggcg tgaacgcctc
    13561 ctgcacacgt ttggcaatct gacgctgctg acgcagccga tgaacagcgc gctcagcaac
    13621 ggcccctatg cagatcaata cgatcagaac ggaacgcgta ccgcggaagg caagcgcagc
    13681 ggtctgcgtg atgggttgtt gagtttgaat agttggtttc agcgccctga aatcaccgag
    13741 tggaatgacg cacggattga ggaacgcggc gcacaattgc tggagcaggc tgtggcttgc
    13801 tggcctcatc cgccagcgga acccgcaaag atttctggtc agcaagttgc cgcaggcgtt
    13861 caagagaatc ccacaccatg atcttttcct tgctggcaac gctgatcgcc tatcttgtgg
    13921 gctcgctgag cttcgccgtc atcgtcagcc gcgccatggg cctgtcggac ccgcgcactt
    13981 acggcagcgg caacccgggc gcgaccaacg tgcttcgctc cggctcaaag cccgcggcca
    14041 tcgtcacgct gctgctcgac gcggccaagg gctggctgcc ggtgatgctg gtcaagtggt
    14101 tcgggccggc gtgggactta agcgaaggca cgcaggcgct ggcggggctg gcggcctttt
    14161 taggccatct gtacccggtg tttttcgcct tcaagggcgg taagggtgtg gcgacggcgg
    14221 tgggcgtgct gctggccttc cagccgctgc tggcgctggc cacgctgctc acctttgcca
    14281 tcatcgtgtt ttttacccgc tacgtgtcgc tggcgtcgat ggtggcggcg gtgttcgcgc
    14341 cggcttatta cctgatcggc gacggcgtgg cctggacggc ttcgggcgcc aagacgctgg
    14401 cactgatcgc catggggctg ctgctgatct ggcgccaccg cgagaacatc cagcgcttgc
    14461 tggcgggcac cgaatcgaaa ttggggcaga agaaaacatg agcacgatcg caagacacgg
    14521 cgtggccgcg cgctattccg aagcatcggt tttcaacggc gtcgtctacc tggccggcat
    14581 ggtgccggaa ggcgcggcga ccgacatccg cagccagacg caggatgtgt tggcgcagat
    14641 cgacgcccac ctggccgctc agggcagcga caagacgcgc ctgttgcgcg tgcagatcta
    14701 cctgtctgac atcgccgaca tcggcgccat gaacgaagtg tgggacgcct gggtcgcgcc
    14761 cggccacgcc ccgccgcgcg ccacggtgca agctgcgctt gcagacgcgg cgtggaaaat
    14821 cgaagtcgtg gcgaccgccg cgcagcggtg atgattgctg caaaataaat agctgcccgc
    14881 gcttacccat caagcgccag cgccccaaaa gacaaaaaag ggctgcgaag caagcccacc
    14941 caatggccgc ggagcaggcc atgctggaac gccgcggccg gggtcccccc ggccgccagc
    15001 gttgtccccc ttgggggaag gcgcgccagc gccacagggg gttacctttt tatcagcatc
    15061 atctgatcgc catcgcgccg catgttgaag cgattcagaa agctgttgcc gagcaaaatc
    15121 gccggcatcg ccgcgggcgt gacgacggta tcgacctcgt acgccaccac gtcgcccacg
    15181 cgcagggtgt tgatcttgaa cagccagccg ggcgccgcgc cgttggcggt gttcatcatc
    15241 acttgccggc cgcttttgta gttcaggccc agccggtcgg cctcgccctg gccgatggcg
    15301 acgaccgagg cgccggtgtc caccatgaac tgcaccggcc ggttgttgat gctgccctgg
    15361 gcgatgaaat ggccgcgccc gtcggccgtc agcgcaatgc ggtcgccgcc gctgggcgcc
    15421 gcgccgccca cgctggccgg cgcttcgccc acgcgcaacg tgacgcggcg gccatcgacc
    15481 agcaccacgg cctgatcacc gctggtggaa atcaccttga cgccctggtg cgtctcgccc
    15541 ggcgccaccg cgcgcggcgc accgccgccg acgatcagca gcgccttgct gccgagcatg
    15601 ccttgcaggg cgacggtttg ggctgacgcg ccaagcgtga atgtcgcgag cattgcggcg
    15661 agcagcggtg cgcgaaaggg cacggccctc accccaaccc tgtcccagaa ggcgaggggg
    15721 ttttctttgg ggcgatgcgc cgcgggctca gtcacgaaag ttgtcgaacg acagcggcaa
    15781 atccgccatg tccttgcgca ggagtgccat caccgcttgc aggtcgtcgc gcttggcgcc
    15841 ggtcacgcgc acgctgtcgc cctggatggc gg
//
LOCUS       DAOMVO010000022        14823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_856_length_14823_cov_15.524106, whole genome shotgun sequence.
ACCESSION   DAOMVO010000022 DAOMVO010000000
VERSION     DAOMVO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_14823_cov_15.524106"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1965
                     /locus_tag="PKV30_02320"
     CDS             430..1965
                     /locus_tag="PKV30_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRL29086.1"
                     /translation="MNNWNTMWDSLQGSMGVHIPQVLGALAIFIIGWFAAALVKAGAR
                     KGLAALKVNQRFSGTTGKPVDIEGVIALVLFWVVMLLTLAAVFNALDLSMVSGSFAAL
                     TTQLFEYAPRILGALLLALLAWLVATLVRGVTQKVLDRTTLDEKLSEHANMSPLSDSL
                     SNALFWLVILLFVPAILGTLQMDGLLSPLREMTGKALDMLPNIVAALVLGGVGWVVAT
                     VLRNLTTNLLRSAGVDQIGNKAGLASTVQLSSLAGLLVFIVVFVPALIAALDALRIEA
                     ISRPATEMLALLMDAVPRIIAAGLILLITWLVASFAARLLASLLANVGFDTLPAKMGL
                     QHAFTRTPPSALVGRIALVFAMLFATVEAGNQLGFHRFSDMIMTFIEFAGDVLLGSAI
                     LVIGFMLANVAYEAITRASGNGEGSALAKVARFAILGIVLAMGLRAMGIADDIVNLAF
                     GLTLGAIAVAVALAFGLGGRDAAGRLASRWVDRLGKPKAAEPPSMITRPLDSTTAPPN
                     DLP"
     gene            2072..2212
                     /locus_tag="PKV30_02325"
     CDS             2072..2212
                     /locus_tag="PKV30_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29087.1"
                     /translation="MSAPAHDDQAHPEDPIEAVTKHIPVVIPVAGAILIFLLAFIAVK
                     MA"
     gene            2491..4368
                     /locus_tag="PKV30_02330"
     CDS             2491..4368
                     /locus_tag="PKV30_02330"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRL29088.1"
                     /translation="MNAPLTPGMVKDLNVPDYVTNAKLIAWVGDMVALCKPERVYWCD
                     GSDAEYDRLCQQLVDAGTFKKLNPAKRPGSFLAWSDPSDVARVEDRTFICSEKQEDAG
                     PTNNWTPPAEMRAKLQPLFDGCMRGRTMYVIPFSMGPLGSPIAHIGVELSDSAYVAVN
                     MKLMTRMGKGAIDVLGDNGDFVPCVHTVGAPLAAGEKDQTSWPCNPDVKYIVHYPETR
                     EIWSYGSGYGGNALLGKKCFALRIASSMGRAEGWLAEHMLILGVTSPEGQKYHVAAAF
                     PSACGKTNFSMLVPPAAFEGWKVTTIGDDIAWIKPRADGKLVAINPEAGYFGVAPGTN
                     TLTNPNCMASLNENVIFTNVALTDDGDVWWEGMEKDQGALPAHLIDWQGHDWTPEDAN
                     AGKKAAHPNARFTVGAGNNPALDSAWDDPAGVPIDAFIFGGRRSTTVPLVTEARNWQE
                     GVYMAATMGSETTAAAFGAQGVVRRDPFAMLPFCGYNMADYFGHWLQMGEQLQAAGAQ
                     LPAIYCVNWFRKGEDGKFVWPGYGENMRVLKWIIDRAERRVAGEQTMFGITPAYGEIN
                     WKGLAFTPEQFATVTRIDKAAWEQEFALHDELFAQLAQGLPPALPQTRQALQERLAAV
                     A"
     gene            complement(4531..4707)
                     /locus_tag="PKV30_02335"
     CDS             complement(4531..4707)
                     /locus_tag="PKV30_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29089.1"
                     /translation="MLTLLNQLFSPAKPAAAVPSRQGVASNLMDRAEACAGQDPQRAA
                     ELRQAAVAYLGVVR"
     gene            4876..5718
                     /locus_tag="PKV30_02340"
     CDS             4876..5718
                     /locus_tag="PKV30_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL29090.1"
                     /translation="MNIQLLSDLHLEENTAFQPQPAPDADLLVLAGDIGSYQRGSALP
                     ALSDTDFGLARFAGWPVPVIYVPGNHEYDSLDFDATHARLRATCERLGLIWLERETRV
                     LQGVRFIGTTLWTDFDALAPRGPTATLTEQLQAREKAYRAANYYLRFAAATRHGEPFM
                     AEQTRAQALVCQDWLRAALAEPCDGPTVVVTHFAPSLQSADPRYGLTPGTAGFCNALD
                     DLLPLADVWLHGHLHCAIDYRDASGRCRVVANPLGYADKGEQAAFKPRWLIEVPRGAP
                     RPAI"
     gene            5790..6212
                     /locus_tag="PKV30_02345"
     CDS             5790..6212
                     /locus_tag="PKV30_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRL29091.1"
                     /translation="MKILLAVDGSPYTKKMLAYLTTHLEMFSGDVEFTALTVQGTLPP
                     RARAAVGKEVVDTYYAEEAKKVLEPVIKFLTRHNLKVKSLSKIGNAGDTIAKTAESGN
                     YDLVIMGSRGHGALGGLVMGSVATRVLAHCQTPVLLVR"
     assembly_gap    6488..6587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6653..7444)
                     /locus_tag="PKV30_02350"
     CDS             complement(6653..7444)
                     /locus_tag="PKV30_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL29092.1"
                     /translation="MLVPTYTMLGHGPVVLMLHGAGGGFRSFAPQVETLASLGFRAVA
                     WNMPGYGPSPPIEPYSFKGLAESAVALIESLAPVTDGAPVALLGHGMGGMVAQEVTLR
                     RPDLIGQLVLVATAAAVQPGDGYNRYVEQGLAWLAEGRPMSEIADTLLPRLVGPGALP
                     AGVQLAAHCQAQVHAATWRRALQAMASFDRRPALGLIHVPTMLIAGEHDSVAPPAQME
                     AMAAAITGAQCVPVPHSGHLPHLERPEEFDTLLLDFLRHAKAWLH"
     gene            7674..8183
                     /locus_tag="PKV30_02355"
     CDS             7674..8183
                     /locus_tag="PKV30_02355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRL29093.1"
                     /translation="MHLPTQAAAPAFSPLEFRAALGMFATGVTIVSARAPTGELVGLT
                     ANSFNSVSLDPPLVLWSLSQRSLSLDAFRTGSHYAINVLAADQHELALRFATPLSDRW
                     DGVAWTPGVAGAPLITGAMAHFECFNKSRYEEGDHVIFVGQVEQVAHRPEATPLLFHG
                     GRFYTEHPL"
     gene            8229..9110
                     /locus_tag="PKV30_02360"
     CDS             8229..9110
                     /locus_tag="PKV30_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL29094.1"
                     /translation="MDTPEDPGTVTFEQHRRLLLAMGYRMLGSRAEAEELVQDTWLRW
                     RTADTRALRSPRAWLITAATRLAIDRLRHARRERMHYEGPWLPEPWAQETTADPADAA
                     AELLDDVSMALMLLLERLTPGERAALLLHDVLDCSHAEVAAVLGASPATCRQWLHRAR
                     QRVRQPAARQAPSAVEHGELLTHFVTALQAQDHTALLALLADDVRWTADGGGAVRAAR
                     RPALGPRAVSRLASAVWRLWLGRQARDPAWLNGAPALVLRVPDGDVSGALLITARAAR
                     IARIDLILNPRKLERLP"
     gene            9107..9550
                     /locus_tag="PKV30_02365"
     CDS             9107..9550
                     /locus_tag="PKV30_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL29095.1"
                     /translation="MNPRLDFPNAAPAAFRAMLTLQQQADRSALAPRLLHLVKLRASQ
                     INGCAYCIALHVTQAEQASEAPQRLHLLPAWRESPGYDARERAALAWAEALTLVHRSH
                     APDTDYEAVRAQFNEAETVELTLAIAAINAWNRLCIAFRSQPALA"
     gene            9547..9954
                     /locus_tag="PKV30_02370"
     CDS             9547..9954
                     /locus_tag="PKV30_02370"
                     /inference="COORDINATES: protein motif:HMM:NF039463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6463 family protein"
                     /protein_id="HRL29096.1"
                     /translation="MSTSSTARLRSARWGGTGLIALGLAHLAAGVPDLLAASPGWAGG
                     GLWTLTHWAPVAQQSAESLASGLTFWITLGSCAVPLVLLGALLRALARQGVAPPWWLG
                     VALAVWGGVATLVMPPSGFVLVPVLAALLGWRR"
     gene            10006..10929
                     /locus_tag="PKV30_02375"
     CDS             10006..10929
                     /locus_tag="PKV30_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL29097.1"
                     /translation="MPSPQPRHEHLDGQAIVILLVCCAFWGVQQVLVKATLPELAPVF
                     QAALRFVGATVLLLLWCAWRRVPLFARDGTLWPGLLAGMLFAAEFAAMYLGLQHTTAS
                     RLTVFLYTSPFWVALLLTLLVPAERLRRVQWVGLICAFVAVAFALREGFVRGGAGVTW
                     RGDLLGLAAGALWGLTTVTIRASRLVQVSPEKMLFYQVAVSAVTLPLLSAALGEPWHW
                     QWSAFAGASIAVQTVIGAFVSYLVWMWLLAHYPATKISAFVFLTPIFALIAGALWLGE
                     TVTPTLLLSLALVAVGIVLVNRRAPAPLQGK"
     gene            complement(10977..11717)
                     /locus_tag="PKV30_02380"
     CDS             complement(10977..11717)
                     /locus_tag="PKV30_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL29098.1"
                     /translation="MPSTLPADVRAAFTPTGALRASINLGNPILAGRNAAGEPAGVSI
                     DLARAFAERLGVGCELVVFDKAQASVEAVRASQADVGFFAIDPLRGEGIRFTAPYVLI
                     EGAYLVRDDAPLKDNAEVDAAGRRVMVGQGSAYDLFLSRELKHATIERAASSPAVVPT
                     FVEKNADVAAGVKQQLEADAARIGGLRLLPGRFMVIQQAMGLPAGRGDAAQGALAAFV
                     EDMKASGFVADALQRHGIQGAAVAPIAG"
     gene            11824..12825
                     /locus_tag="PKV30_02385"
     CDS             11824..12825
                     /locus_tag="PKV30_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRL29099.1"
                     /translation="MQFKDYYQILGVTKSATQDDIKKAYRKLARKYHPDVSKEKHAAE
                     RMAEVNEANTVLGDAEKRAAYDTVGAQAWAAGARSGDDVRPPPGWNSGYEYTEGGGAG
                     ADAFRQHFGGDSGDYSEFFEQMFGRAGAGGGARGRGRARADAPMRGEDQHARIELELI
                     DAYKGAERSLNLRGARVDDSGHVVPEERTLQVKIPKGVKEGQLVRLAGQGSPGFNGGP
                     AGDLLLEVQFRPDPRYRIDGRDVTQKLRVTPWEAALGGGVAVMTPDGATVEVTVPAGS
                     GGGRKLRLKGRGIPSSTTPGDLYLELEIAVPGAVTPEQKTAWEALKQTYPGFDPRIK"
     gene            13015..13338
                     /locus_tag="PKV30_02390"
     CDS             13015..13338
                     /locus_tag="PKV30_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRL29100.1"
                     /translation="MNPVPLTIPAHLVELLDDAALSLQELARGCAMSPDWVHTHVEAG
                     VLQPAQGGGATEWRFASATLTRARRIAQLEHTFDADPQLAALTTDLMEEVAALRRQLA
                     AFQLI"
     gene            complement(13442..14290)
                     /locus_tag="PKV30_02395"
     CDS             complement(13442..14290)
                     /locus_tag="PKV30_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRL29101.1"
                     /translation="MNRSSAVTRAALALSMGLLAAGAASAQTMVNARVLSSTPVWEQV
                     PVSDCRGAYPGAVRPSGPGTAVGAVVGGLIGSQLGRGSGHIAGAILGTVGGALLGNAA
                     EAQAGYPGACGTRYENRMTGYDVSYEVAGRPYRTRMAQDPGAWVQVPDPGYGSYGGYG
                     GAPDVQSYPVNPAPMAGYPLPAYPAAGEGSGVVTAPPVYGGYPGQYPNAYPGYPAPGY
                     AQTYPAPVYAQPVYPQPVYQAAPVYVRPAPVYAAPVGVNLSIGGVIGGHRHSRGGWGV
                     GIGTGF"
     gene            14549..>14823
                     /locus_tag="PKV30_02400"
     CDS             14549..>14823
                     /locus_tag="PKV30_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL29102.1"
                     /translation="MNAHEVDLAPPGAGADDAVRVVVVDDQPDAADMLAAALELEGCQ
                     ARTAQSGEEALPLIDQFQPHAVLLDVSMPGGMDGLELTRLLRERYGDA"
BASE COUNT         2272 a         5132 c         5029 g         2290 t
ORIGIN      
        1 ccagcgccat caggccgttg atgtcgcgcc gcacgttgaa cttgctgccc tgctgggcgg
       61 ccagcgtcag cacgaagtgc gagcacatca agtccagcac cggggccgtg gccaagccgg
      121 gcgagggcag caggcccgcc tgttcacgca ccgagatcgt ccgtttcgcc atcagccgcc
      181 tcccttgggg ttgagcgctt gcacaaatgt caccataacg caatcgcggc gggcgcggaa
      241 gacaggggcg ggggcgttct gacccacggt gtggcacccc aaacacaact attgcgcaga
      301 ttgcgccgcc aaatggcgtc caaaggaccg cgcctgcggc gccgtcctac aagccgggcg
      361 attgacggtg tcacacccgc aggccatcat taacgaaagt tgtcagtttc ttaagcgagg
      421 agtgagcaaa tgaacaactg gaacacgatg tgggattcgc tgcaaggctc gatgggcgtg
      481 cacatcccgc aggtgctggg cgcgctggcg atcttcatca tcggctggtt tgccgccgcg
      541 ctggtgaagg cgggtgcgcg caagggcctg gccgcgctca aggtgaacca gcgcttttcg
      601 ggcaccaccg gcaagccggt cgacatcgag ggcgtgatcg cgctggtgct gttctgggtg
      661 gtcatgctgc tcaccttggc ggcggtgttc aacgcgcttg atctgtccat ggtgtccggc
      721 tcgttcgcgg cgctcaccac gcagttgttt gaatacgccc cgcgcatcct gggcgcgctg
      781 ctgctggcac tgctggcctg gctggtcgcc acgctggtgc gcggcgtgac gcagaaggtg
      841 cttgaccgca ccacgctgga cgaaaagctg tcggagcacg ccaacatgtc gcccctcagc
      901 gacagcctgt cgaacgcgct gttctggctg gtgattttgc tgttcgtgcc ggccatcctg
      961 ggcacgctgc agatggacgg cctgctgtcg ccgctgcgcg agatgacggg caaggcgctg
     1021 gacatgctgc ccaacatcgt cgccgcgctg gtgctgggcg gcgtgggctg ggtggtggcc
     1081 acggtgctgc gcaacctgac gaccaacctg ctgcgctcgg ccggcgtcga ccagatcggc
     1141 aacaaggcgg gcctggccag cacggtgcag ctgtcgagcc tggccgggct gctggtgttc
     1201 atcgtggtgt tcgtgccggc gctgattgcc gcgctggacg cgctgcgcat cgaggccatt
     1261 tcgcgccccg ccaccgaaat gctggcgctg ttgatggacg cggtgccgcg catcattgcc
     1321 gccgggctga tcctgctgat cacctggctg gtggcctcgt ttgccgcgcg cctgctggcc
     1381 agcctgctgg ccaacgtcgg ctttgacacg ctgcccgcca agatgggcct gcagcacgcc
     1441 ttcacgcgca cgccgccgtc ggcgctggtg ggccgcatcg cgctggtgtt tgccatgctg
     1501 tttgccaccg tcgaggccgg caaccagctg ggctttcacc gcttcagcga catgatcatg
     1561 accttcatcg agtttgccgg cgacgtgctg ctgggctcgg ccatcctggt catcggcttc
     1621 atgctggcca acgtggcgta cgaggccatc acccgcgcca gcggcaacgg cgaaggcagc
     1681 gcgctggcca aggtggcgcg ctttgccatc ctgggcatcg tgctggcgat ggggctgcgc
     1741 gccatgggca ttgccgacga catcgtcaac ctggcctttg gcctgacgct gggcgccatc
     1801 gcggtggccg tggcgctggc ctttggcctg ggcgggcgcg acgccgccgg ccgcctggcc
     1861 tcgcgctggg tcgatcggct gggcaagccc aaggccgccg agccgccctc gatgatcacc
     1921 aggccgctgg acagcacgac cgcgccgccg aacgacctgc cctgaccctc ggcgacacga
     1981 cacgcccacg gccccggaat ccaccccggg gccgtttttt catgcgtacg cctatagaat
     2041 cgttgatccc ccagttgaga ggctcattcc catgtcagcg cccgcccacg acgatcaggc
     2101 ccatcccgaa gaccccatcg aggccgtcac caagcacatc ccggtggtga ttccggtggc
     2161 cggcgccatc ctgatctttc tgcttgcctt catcgccgtg aagatggcct gacggccggc
     2221 ttttctgcat caaaattggc tctggcgcac gccagacaag cgccagcagc tcctgaaagc
     2281 gtagcgaaac cactcaagcg cagggtggat cgcccgcgtc agccagacgg cgggccgatt
     2341 tcataaaatc gtcacttcgg ccatcggctg atcgcaccgc ccactgggcg gtttttttcc
     2401 agcgacgggc cgcccagccc cggttcgaat ccgctttccc aaggttgcgt gctttcggaa
     2461 agagcgattt tcaacttagg agctttgccg atgaacgccc ccctgacccc cgggatggtc
     2521 aaggacttga acgtccccga ctacgtcacg aacgccaaac tgatcgcctg ggtgggcgac
     2581 atggtggcgc tgtgcaagcc agagcgcgtc tactggtgcg acggcagcga tgccgaatac
     2641 gaccgcctgt gccagcaact ggtcgatgcc ggcaccttca agaagctgaa cccggccaag
     2701 cgcccgggct cgttcctggc ctggtccgac ccgtccgacg ttgcgcgcgt cgaagaccgc
     2761 accttcatct gctcggaaaa acaggaagac gccggcccca ccaacaactg gacgccgccg
     2821 gccgaaatgc gcgccaagct ccagccgctg ttcgacggct gcatgcgcgg ccgcaccatg
     2881 tacgtcatcc cgttcagcat gggcccgctg ggcagcccca tcgcccacat cggcgtcgag
     2941 ctgtccgact cggcctatgt cgccgtcaac atgaagctga tgacgcgcat gggcaagggt
     3001 gccatcgacg tgctgggtga caacggcgac ttcgtgccgt gcgtgcacac cgtgggcgcg
     3061 ccgctggccg cgggcgaaaa agaccagacg agctggccgt gcaaccccga tgtgaaatac
     3121 atcgtgcact accccgaaac gcgcgagatc tggtcgtacg gctcgggcta cggcggcaac
     3181 gcgctgctgg gcaagaagtg ctttgcgctg cgcatcgcct ccagcatggg ccgtgccgaa
     3241 ggctggctgg ccgagcacat gctgatcctg ggcgtgacct cgccggaggg ccagaaatac
     3301 cacgtcgccg ccgccttccc ctcagcctgc ggcaagacca acttctcgat gctggtgccg
     3361 cccgccgcct tcgagggctg gaaggtcacc accatcggcg acgacatcgc ctggatcaag
     3421 ccgcgggccg acggcaagct ggtcgccatc aaccccgagg cgggttactt tggcgtggcg
     3481 cccggcacca acacgctcac aaaccccaac tgcatggcca gtctgaacga gaacgtgatc
     3541 ttcaccaacg tcgcgctgac cgacgacggc gacgtgtggt gggaaggcat ggaaaaagac
     3601 cagggcgcgc tgcccgcgca cctgatcgac tggcaaggcc acgactggac gcccgaagac
     3661 gccaacgccg gcaagaaggc ggcgcacccc aacgcgcgct tcaccgtcgg cgccggcaac
     3721 aacccggcgc tggattcggc ctgggacgat ccggccggcg tgcccatcga cgccttcatc
     3781 tttggcggcc gccgcagcac caccgtgccg ctggtgaccg aggcgcgcaa ctggcaggaa
     3841 ggcgtctaca tggccgccac catgggaagc gaaaccaccg ccgccgcctt tggcgcccaa
     3901 ggcgtggtgc ggcgcgaccc gttcgccatg ctgccgttct gcggctacaa catggccgac
     3961 tactttggcc actggctgca gatgggcgag caactgcagg ccgccggcgc ccagctgccc
     4021 gccatttact gcgtcaactg gttccgcaag ggcgaagacg gcaagttcgt ctggcccggc
     4081 tacggcgaga acatgcgcgt gctgaagtgg atcatcgacc gcgccgagcg ccgcgtggca
     4141 ggcgagcaga ccatgttcgg catcacgccg gcctatggcg agatcaactg gaagggcctg
     4201 gccttcacgc ccgagcagtt cgccaccgtc acccgcatcg acaaggcggc gtgggagcag
     4261 gaattcgccc tgcacgacga gctgttcgcg caactggcgc aaggcttgcc gccggcgctg
     4321 ccgcaaacgc gccaggcgct gcaggagcgg ctggcggcgg tggcctgatc cagaccgcgc
     4381 catgcaaaaa ggcccgtcag cgacgggcct ttttttctgc cggcacgcgc acgaaggtgc
     4441 tgacggcgac atttaatgca aaaagaggcg ccagcgcttg tctgacaagc gcgagaagct
     4501 atggattttg aagtatcgcg gggcgggcgc tcagcgcacc acgcccaggt aggccacggc
     4561 ggcctggcgc agctcggcgg cgcgctgggg gtcctggccg gcgcaggctt cagcgcggtc
     4621 catcaggttg gaggccacgc cctggcggct cggcacggcg gcagcgggtt tggcggggga
     4681 gaaaagctgg ttcagcagcg tcaacatggt gttttgcgcc cggtggtttg aagggcgccc
     4741 tcttgaagtg gcttttggcc gatgcgccga ttgtcgccga tcctagggtt tttacctagg
     4801 cgctggagtc caatccggcc gcgccggggg ctcggcgtga caaactggca ggcttgcgtt
     4861 gcttttcgac cattgatgaa catccagctt ctgtccgacc tgcacctgga agagaacacc
     4921 gccttccagc cccagcccgc gccggatgcc gacctgctgg tgctggcggg cgacatcggc
     4981 tcgtaccagc gcggttcggc cctgccggcg ctgagcgaca ccgatttcgg cctggcgcgc
     5041 tttgccggct ggccggtgcc ggtgatctac gtgccgggca accacgaata cgacagcctg
     5101 gactttgacg ccacgcacgc gcgcctgcgc gccacctgcg agcggctggg cctgatctgg
     5161 ctggagcgcg aaacgcgggt gctgcagggc gtgcgcttca tcggcaccac gctgtggacc
     5221 gactttgacg ccctggcgcc gcgcggcccc acggcgacgc tgaccgagca gctgcaggcg
     5281 cgcgaaaaag cctaccgcgc ggccaactac tacctgcgct ttgccgccgc cacgcgccat
     5341 ggcgagccct tcatggccga gcagacgcgc gcgcaggcgc tggtctgcca ggactggctg
     5401 cgcgccgcgc tggccgaacc gtgtgacggc cccacagtgg tggtgacgca ctttgcgccc
     5461 agcctgcaaa gcgccgaccc gcgctacggc ctgacgcccg gcaccgccgg gttctgcaac
     5521 gcactggatg atctgctgcc gctggccgac gtgtggctgc acgggcacct gcactgcgcc
     5581 atcgactacc gagacgccag cggccgctgc cgcgtggtgg ccaatccgct gggctatgcc
     5641 gacaagggcg agcaggcggc gttcaagccg cgctggctga tcgaggtgcc gcgcggcgcg
     5701 ccccgcccgg ccatttgatg ccgcgcagcc cgcgcggcgc gctcagggct tagacttgca
     5761 cgatccgcaa ctgcaactga ggacgaccga tgaaaatcct gctcgctgtc gacggcagcc
     5821 cctacaccaa aaagatgctg gcctacctga ccacgcacct ggagatgttc tcgggcgacg
     5881 tcgagttcac cgccctcacg gtgcagggca cgctgccgcc ccgggcgcgc gccgccgtcg
     5941 gcaaggaggt ggtggacacc tactacgccg aagaggcgaa aaaggtgctg gagccggtga
     6001 tcaagttcct cacgcgccac aacctgaagg tgaaaagcct gtcgaagatc ggcaacgcgg
     6061 gcgacaccat cgccaagacg gccgaaagcg gcaattacga cctggtcatc atgggctcgc
     6121 gcggccatgg cgcgctgggc gggctggtga tgggctcggt cgccacgcgg gtgctggcgc
     6181 attgccagac gccggtgctg cttgtgagat gacccccctg agccgctgac gcggcttccc
     6241 cccaggggga caacgctggt ggccgggggg accccggcca cggcgttcct gggtggcctg
     6301 ctccgcggcc ctcggaaagg gcttgctgcg cagccctgaa agcgttttct ggcgctggcg
     6361 cttgatgggc cagcgcaagc agctatggtt ttgagtgcac tcttgagtgc cagcggaacc
     6421 ttcaggcgat cttttgcgcc atctctgcgc cctttgcgtt caaaggtttt ttaagggcac
     6481 atccacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt gcgccatctc
     6601 tgcgcccttt gcgttcaaag gttttttaag ggcacatcca cgccccgcaa gctcaatgca
     6661 gccacgcctt ggcgtggcgc aaaaagtcga gcagcaaggt atcgaattcc tccggccgct
     6721 ccaggtgcgg caggtggccg ctgtggggca cgggcacgca ttgcgcgccg gtgatggcgg
     6781 cggccatggc ttccatctgc gcgggcggcg ccacgctgtc gtgctcgccg gcaatcagca
     6841 tcgtcggcac gtggatcagg ccgagcgcgg ggcggcggtc gaacgaggcc atggcctgca
     6901 gcgcgcgccg ccaggtggcc gcgtgcacct gcgcctggca gtgcgccgcc agttgcacgc
     6961 cggccggcag cgcgccgggg ccgaccaggc gcggcagcag cgtgtcggcg atctcgctca
     7021 tcggccggcc ctcggccagc caggccaggc cctgctcgac gtagcggttg tagccatcgc
     7081 ccggctgcac ggcggcggcg gtggccacca gcaccagctg gccgatcaga tcgggccggc
     7141 gcagcgtcac ctcctgcgcc accatgccgc ccatgccgtg gccgagcagc gccaccggcg
     7201 cgccatcggt caccggcgcg agcgattcga tcaaggccac cgcgctttcg gccaggccct
     7261 tgaagctgta cggctcgatc ggcgggctgg ggccgtagcc gggcatgttc cacgccacgg
     7321 cgcgaaaacc caggctggcc agcgtctcga cctgcggcgc gaacgagcga aacccgccgc
     7381 cggcgccgtg cagcatcagc accacggggc cgtggccgag catggtgtag gtggggacga
     7441 gcatctgctg atactaccca tgtcgggcga atgactgcgc ctgcggcgca taacttcggc
     7501 ctgcggcctt caatgacgcg ccctgtgggc gtcatgactc aagtcatgcg cccacagggc
     7561 gcgtcatcga gcgcagcgag gtcatggcgc gccagcgccg tcatgccacg tgaccccgga
     7621 gacaacgcgg gcgcaggctg ctcgctatca tggcccgctg tctcgcgccg cccatgcacc
     7681 tgcccaccca agccgccgcc cctgccttct cgccgctcga attccgcgcc gcgctgggca
     7741 tgttcgccac cggggtgacc atcgtcagcg cgcgcgcgcc gacgggcgag ctggtgggct
     7801 tgacggccaa ttcgttcaac tcggtctcgc tcgatccgcc gctggtgctg tggagcctga
     7861 gccagcgctc gctgtcgctg gacgcctttc gcaccggctc gcactacgcc atcaacgtgc
     7921 tggcggccga ccagcacgaa ctggcgctgc gctttgccac cccgctgtcc gaccgctggg
     7981 acggcgttgc ctggacgccc ggcgtggccg gcgcgccgct gatcaccggc gccatggcgc
     8041 atttcgagtg cttcaacaaa agccgttatg aagaaggcga ccacgtcatc ttcgtcggcc
     8101 aggtcgaaca ggtggcgcac cggcccgaag cgacgccgct gctgtttcat ggcgggcgct
     8161 tttacaccga gcacccgctg tagcgccggc ggcgtcacaa tccacggtcg ccatgcgtct
     8221 tgatcggcat ggacacccct gaagacccgg gcaccgtcac cttcgagcag caccgccgtc
     8281 tgctgctggc gatgggctac cgcatgctcg gcagccgcgc cgaagccgag gagctagtgc
     8341 aagacacctg gctgcgctgg cggacggcgg acacccgggc cctgcgcagc ccacgggcct
     8401 ggctcatcac cgccgccacc cgcctggcca tcgaccgcct tcgccatgcg cggcgcgagc
     8461 gcatgcacta cgaagggccc tggctgcccg aaccctgggc gcaggaaacc acggccgacc
     8521 cggccgacgc ggccgccgag ttgctggacg acgtctcgat ggcgctgatg ctgctgctgg
     8581 agcgcctgac gcccggcgag cgcgcggcgc tgctgctgca cgacgtgctc gactgcagcc
     8641 acgccgaagt cgccgccgtg ctgggcgcca gccccgccac ctgccgccag tggctgcacc
     8701 gcgcgcgcca gcgggtgcgt cagccggccg cgcgccaggc cccgagcgca gtcgagcacg
     8761 gcgagctgct gacgcacttt gtgacggcac tgcaagcgca ggaccacacc gccctgctgg
     8821 cgctgctggc cgacgacgtg cgctggaccg ccgatggcgg cggcgcagtg cgcgcggccc
     8881 ggcgcccggc gctggggccg cgtgccgtat cgcgcctggc cagtgccgtc tggcgcctct
     8941 ggctcggccg gcaggcgcgc gacccagcct ggctgaacgg cgcgccggcg ctggtgctgc
     9001 gtgtgcccga tggcgacgtc agtggcgccc tgctgatcac cgcccgcgcc gcgcgcattg
     9061 cgcgcatcga cctgatcctg aacccccgca aactggagcg cttgccatga accctcgcct
     9121 cgactttccc aacgccgccc cggccgcatt tcgcgccatg ctgacgttgc agcagcaggc
     9181 cgaccgcagc gcgctggcgc cgcgcctgct gcacctggtc aagctgcgcg cctcgcagat
     9241 caacggctgc gcctattgca tcgcgttgca cgtgacgcag gccgagcagg cgagcgaggc
     9301 gccgcagcgc ctgcacttgc tgcctgcctg gcgcgaatcg cccggctacg acgcgcgcga
     9361 acgcgcggcg ctggcctggg ccgaggcgct gaccctggtg caccgcagcc acgcgcccga
     9421 taccgactac gaagccgtgc gcgcgcaatt caacgaggcc gaaacggtgg agctgacgct
     9481 ggccattgcc gccatcaacg cctggaaccg cctgtgcatc gcgtttcgca gccagccggc
     9541 acttgcatga gcacgtcttc gaccgcgcgc ctgcgcagcg cccgctgggg cggcaccggc
     9601 ttgatcgccc tgggcctggc ccacctggct gcgggcgtgc ccgacctgct ggccgcgtcg
     9661 cccggctggg ccggcggcgg gctgtggacg ctgacgcatt gggcgcccgt ggcccagcaa
     9721 tcggctgaaa gcctggccag cgggcttacg ttctggatca cgctgggcag ctgcgcggtg
     9781 ccgctggtgc tgctgggcgc gctgctgcgg gcgctggcgc ggcaaggcgt ggcgccgccc
     9841 tggtggctgg gcgtggccct ggcggtgtgg ggcggggtcg cgacgctcgt catgcccccg
     9901 agtggctttg tgctggtgcc ggtgctggca gcgctgctgg gctggcggcg ctgagcgcgc
     9961 gcggtgcgcc ggggcgaacg cccagatggc gcagaatcgc cccccatgcc ctcaccacag
    10021 ccgcgccacg aacacctcga cggccaggcc atcgtcatcc tgttggtgtg ctgcgccttc
    10081 tggggcgtgc agcaggttct ggtgaaggcc acgctgcccg agctggcgcc ggtgtttcag
    10141 gccgcgctgc gctttgtcgg cgccaccgtg ctgttgctgc tgtggtgcgc gtggcgccgc
    10201 gtgcccttgt ttgcgcgcga cggcacgctg tggcccgggc tgctggcagg catgctgttt
    10261 gcggccgagt ttgccgccat gtacctcggc ctgcagcaca ccacggcctc gcgcctgacg
    10321 gtgttcttgt acacctcgcc gttctgggtc gcgctgctgc tgacgctgct ggtgccggcc
    10381 gagcggctgc ggcgcgtgca gtgggtcggc ctgatttgcg cctttgtcgc cgtggcgttt
    10441 gcgctgcgcg aaggctttgt tcggggcggc gccggcgtga cctggcgcgg cgatttgctg
    10501 ggcctggcgg cgggcgcgct gtgggggctg accacggtga ccatccgtgc cagccggctg
    10561 gtgcaggtgt cgccggagaa gatgctgttc taccaggtgg ccgtcagcgc cgtcacgctg
    10621 ccgctgctgt cggcggcgct gggcgagccg tggcactggc agtggagcgc gtttgccggc
    10681 gcctccattg cggtgcagac ggtgattggc gccttcgtca gctatctggt gtggatgtgg
    10741 ctgctggcgc actacccggc caccaaaatt tccgccttcg tgtttctgac gcccatcttc
    10801 gcgctgatcg ccggcgcgct gtggctgggc gaaacggtga cgcccacgct gctgctgtcg
    10861 ctggcgctgg tggcggtcgg catcgtgctg gtcaaccggc gcgcgccggc gccgttgcaa
    10921 ggtaaatagg gcgctggcgc ttgtccatca agcgcggaca gctatcaaat tgagagttat
    10981 ccggcgatcg gcgccaccgc cgcaccctga atgccgtggc gctggagcgc atcggcaacg
    11041 aagccgctgg ccttcatgtc ctcgacaaac gcggccagcg ccccttgcgc cgcgtcgccg
    11101 cggccggccg gcaagcccat ggcctgctgg atcaccatga agcgccccgg cagcaggcgc
    11161 aggccgccga tgcgggcggc gtccgcttcc agctgctgct tcacgccggc ggcgacgtcg
    11221 gcgttttttt caacaaaggt gggcaccacg gcgggcgagc tcgcggcgcg ctcgatggtg
    11281 gcgtgtttca gctcgcgcga caagaacagg tcgtaggcgc tgccctggcc caccatcacg
    11341 cggcggccag cggcatcgac ctcggcgttg tccttgagcg gtgcgtcgtc gcgcaccagg
    11401 tacgcgccct cgatcagcac atagggcgcg gtgaagcgga tgccttcgcc gcgcagcggg
    11461 tcgatggcga agaagccaac gtcggcttgg cttgcgcgca ccgcctcgac cgaagcctgc
    11521 gccttgtcga acacgaccag ctcgcacccc acacccagcc gctcggcgaa ggcgcgcgcc
    11581 aggtcgatgg agacgcccgc cggctcgccc gccgcgttgc gcccggccag gatcgggttg
    11641 cccaggttga tggaagcgcg cagcgcgcca gtgggggtga aggcggcgcg aacgtcggcg
    11701 ggcagggtgc tgggcatggc ggtgtttggg tgaaaggaca ggcgctgcat tttgccgccg
    11761 acgggcgcaa taatgtcgcc aaggccgcgc cttcttcgcc ggccaccacc tgaccccaag
    11821 cgcatgcaat tcaaggacta ctaccagatc ctcggcgtca ccaagagcgc gacgcaggac
    11881 gacatcaaga aggcctatcg caagctggcg cgcaagtacc acccggacgt cagcaaagag
    11941 aagcatgccg ccgagcgcat ggccgaggtg aacgaggcca acaccgtgct gggcgacgcc
    12001 gaaaagcgcg ccgcctacga caccgtgggc gcgcaggcct gggccgccgg cgcccgatcc
    12061 ggcgacgacg tgcgcccgcc gccgggctgg aacagcggct acgaatacac cgagggcggt
    12121 ggcgcgggcg ccgacgcctt ccgccagcac tttggcggcg attcgggcga ctacagcgaa
    12181 ttcttcgagc agatgttcgg ccgcgcgggc gccggtggtg gcgcgcgcgg gcgcggccgt
    12241 gcgcgggccg atgccccgat gcgcggcgaa gaccagcacg cgcgcatcga gctggagctg
    12301 atcgacgcct acaagggcgc agagcgcagc ctgaacctgc gcggcgcgcg ggtggacgac
    12361 agcggccacg tggtgccgga agagcgcacg ctgcaggtca agatccccaa gggcgtgaag
    12421 gaaggccagc tggttcgtct ggccggccag ggcagcccgg gcttcaatgg cgggccggcg
    12481 ggcgatctgc tgctggaagt gcagtttcgc cccgacccac gctatcgcat cgacggccgc
    12541 gacgtgacgc aaaagctgcg cgtgacgccg tgggaagcgg cgctgggcgg cggcgttgca
    12601 gtgatgaccc ccgatggcgc gacggtcgaa gtcaccgtgc cggccggttc gggcggcggg
    12661 cgcaagctgc ggttgaaagg gcgcggcatc ccctcatcca ccacgccggg cgacttgtac
    12721 ctggagctgg aaatcgccgt gcccggcgcc gtcacgcccg agcagaaaac cgcgtgggag
    12781 gcgctgaaac agacgtatcc cggcttcgat ccacgtatca aatgaaacac cccctgtgcc
    12841 gccttcggcg tcttccccct ctcctgcggg aggcggacgg cgccagtcac cggggaaacc
    12901 ccggcgacgg cgcccacgtg tggtctgctc cgcgaccatc gggctctttg ggtttctggc
    12961 cttgcgggcg gcgcgccagc gtcatggaca actgattttt ggagagcacc gaagatgaac
    13021 cccgtgcccc tcaccatccc cgcccacctg gtcgaattgc tggacgatgc cgccttgagc
    13081 cttcaagagt tggcgcgcgg ttgcgccatg agccccgact gggtgcacac ccacgtcgaa
    13141 gccggcgtgc tgcaacccgc gcaaggcggc ggcgccaccg aatggcgctt tgccagcgcc
    13201 acgctgacgc gcgcacgccg catcgcgcag ctcgaacaca cctttgacgc tgacccgcaa
    13261 ctggccgcgc tgacgaccga cctgatggaa gaagtggcgg cgctgcggcg gcaactcgcg
    13321 gcgtttcagc tgatttgaac aaaaaaggcc gccagcgctt gccagacaag cgcaaacagc
    13381 tatcaaatca ggaattgaaa acggcgcccg caggcgccgc cgaaagaccg cccgcaggcc
    13441 gtcagaaccc agtgccgatg cccacgcccc agccgccgcg gctgtggcga tggccgccga
    13501 tcacgccacc aatcgacagg ttgacaccca ccggcgccgc atagaccggt gccggccgca
    13561 cgtacaccgg cgccgcctga tacacgggtt gcgggtacac cggctgcgca tagacgggtg
    13621 cggggtacgt ctgggcatag ccgggcgccg gatagcccgg gtaggcgttc gggtactggc
    13681 ccggatagcc gccgtacacc ggcggcgccg tcaccacgcc cgagccctcg cccgctgccg
    13741 ggtaggccgg cagcggatag cccgccatcg gcgccgggtt gaccgggtag ctctgcacat
    13801 cgggcgcgcc gccgtagcca ccgtagctgc catagccggg gtcgggcacc tgtacccagg
    13861 cgcccgggtc ttgcgccatg cgcgtgcggt aggggcggcc ggcgacttcg tagctgacgt
    13921 cgtagccggt catgcggttt tcatagcgcg tgccgcaggc gccggggtag ccggcctgcg
    13981 cctcagccgc gttgcccagc agcgcgccgc ccaccgtgcc cagaatggcg ccggcgatgt
    14041 ggcccgaacc gcggcccagc tggctgccga tcagcccgcc caccacggcg ccgaccgccg
    14101 tgcccgggcc agagggccgc acggcgcccg ggtaggcacc gcggcagtcg gacaccggca
    14161 cctgttccca caccggggtg gacgacagca cgcgcgcgtt gaccatggtt tgcgcgctgg
    14221 cggcgccagc ggccagcagg cccatcgaca gcgccagcgc ggcgcgggtg acagcagacg
    14281 atcggttcat gatttcacac actccaaaga cgcacccatg cgtcggttgc agccccttta
    14341 acgccggcgc caaaacccgg gccgaccatc tttacatgcg ctttgcgcag cgcgggcgac
    14401 agcgtgaaac atttctatgc tccgcgacat agcgcgcttg ggcgggccgg ctgaaaatga
    14461 ctgtgagctg accccccggc atcagccttg gtttacgcgc cttgccgcgc agccccgtcc
    14521 ggcccacgtc cccaggaggg ggtttcccat gaatgcccat gaggtcgatc tcgcgccacc
    14581 cggcgcgggg gcggacgacg ccgtgcgcgt ggtggtggtc gacgaccagc ccgacgccgc
    14641 cgacatgctg gccgccgcgc tggagctgga aggctgccag gcccgcactg cgcaaagcgg
    14701 cgaagaggcg ctgccgctga tcgatcagtt tcagccccac gccgtgctgc tggacgtgag
    14761 catgcccggc ggcatggacg gcctcgagct gacgcgcctg ctgcgcgagc gctacggcga
    14821 cgc
//
LOCUS       DAOMVO010000023        14788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_861_length_14788_cov_14.339849, whole genome shotgun sequence.
ACCESSION   DAOMVO010000023 DAOMVO010000000
VERSION     DAOMVO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_14788_cov_14.339849"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /gene="cysS"
                     /locus_tag="PKV30_02405"
     CDS             complement(<1..996)
                     /gene="cysS"
                     /locus_tag="PKV30_02405"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRL29103.1"
                     /translation="MTLRIYNTLSRGLVDFSPIDPGHVRMYVCGITVYDLCHIGHARS
                     AVAFDVVQRWLRASGYRVTFVRNITDIEDKIIRRALENGETIRQLTDRMIAEMYRDFD
                     ALGVQRPDHDPRATDYVPQMLDIVGKLKDKGLAYQATNGDVNYAVRKFAGYGKLSGKS
                     LDELQAGERVAVDGAKQDPLDFVLWKSTKPTEPEGAKWDSAYGLGRPGWHIECSAMGG
                     ALLGETFDIHGGGADLQFPHHENEIAQSEGAHGVPLAKVWMHNGFVNIDNEKMSKSLG
                     NFFTIREVMEKFDAETIRFFVVRAHYRSPLNYSDVHLADARAALKRLYTALDAVPA"
     gene            1191..1736
                     /locus_tag="PKV30_02410"
     CDS             1191..1736
                     /locus_tag="PKV30_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL29104.1"
                     /translation="MTFFRTFFTPQARAAGLAAALLLAGSAAWADEYAEVQRLQRAGQ
                     TSEALARADKYIAANPRDPQMRFVKAGVLTAAGQAPQAEELLVQLTRDYPELPEPWNN
                     LAVLHASRGQLDKADEALQAALRINPAYATALENLGDVRVRQASEAYQRARQLDGANA
                     RLAPKIEALRGALAGAAKPGG"
     gene            1823..2503
                     /locus_tag="PKV30_02415"
     CDS             1823..2503
                     /locus_tag="PKV30_02415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL29105.1"
                     /translation="MTLSRRTLARLATTLAAAGALGTALPAFAADPRVKFVTSEGDFV
                     VEVYPDKAPKTVENFLQYVKDKHYDGTIFHRVIKNFMVQGGGYDKDYKQKPNRPSIKH
                     EGREALAKGLKNDVGTVAMARTGDPHSATSQFFINVVDNSRLDPVILPAGDPVTFEFQ
                     GQLQKDIPRKNVENHPALYGYTVFGKVVSGMDTVEKIRNTPTGAGGPFPTDVPQKPVV
                     IQSATLVK"
     gene            2520..3032
                     /locus_tag="PKV30_02420"
     CDS             2520..3032
                     /locus_tag="PKV30_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL29106.1"
                     /translation="MPNPQVELHIKDHGVITLELDEAKAPKSVANFLAYVKAGHYDNT
                     VFHRVIKNFMIQGGGFEPGMKQKPTQAPVTNEADNGLKNNQYTVAMARTSDPHSATAQ
                     FFINTVDNDFLNFKAPTAQGWGYAVIGKVVKGENVVDAIKAVPTTRKGFHDDVPRDDV
                     VIEKAVVVGA"
     gene            3044..3835
                     /locus_tag="PKV30_02425"
     CDS             3044..3835
                     /locus_tag="PKV30_02425"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRL29107.1"
                     /translation="MTTVPRFAALPARAHWRTVDFISDLHLKPEEPLTFDAWARYLRH
                     TPADAVFMLGDLFEVWVGDDSALPGSFEAQCGEVLRQASAARDVFLMRGNRDFLVGGD
                     FLAQSGVGDLADPTLLTFDEHTYLLTHGDLLCTDDVAYQQFRQQVRAPEWQSAFLARP
                     LPERQAVARQMRAQSEAQHAAQGVYAQVDADLARRWLAEADATVLIHGHTHRPGDHAL
                     GADARGRPLTQVVLSDWHVTATERRLEVLRLTAQGGLQRVSPGPT"
     gene            3841..4659
                     /locus_tag="PKV30_02430"
     CDS             3841..4659
                     /locus_tag="PKV30_02430"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRL29108.1"
                     /translation="MLTNLTRWVRRAAGGPTPIPDELWRQTMRHHPFLRTLSTGEYAR
                     LRRLSEHFLDRKEFIGAHGLEVTDAMAVAVAAQACLPLLHLGPPDSPEDALQWYDDFV
                     GIVMHPDAAVARRQRTDEAGVMHHYDEVLSGEAMHGGPVMLSWPAVQNAGTDQDAEGS
                     VVIHEFVHKMDMQGGSADGAPPLPRGFMGATSSREAAAHWYRTWAPAYERFREQVTLA
                     QRFGAPAPWLDAYGATAPAEFFAVACEAHFVQPERFALEFPELSAALRAFFKQK"
     gene            complement(4713..6098)
                     /locus_tag="PKV30_02435"
     CDS             complement(4713..6098)
                     /locus_tag="PKV30_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRL29109.1"
                     /translation="MSTAPRRTWSLAQRLMAMVLGFVLVTWVLTAAVAWFVTQHELNE
                     LLDAHLAQTAALLTTGEVDDDDGDAVVRAPTLLHKYQSRVAFQIWRQGKLRARSADAP
                     EQPLAALDVRGLSNQQIGGRIWRVFSTAREEGGQVRDVIQVGEHASARRHVLLASLRG
                     TLIPLLAALPLLAAGIWWAVRRAVRPLERLSASVAARRPQAMDRLPEDDTLPEVRPLV
                     QALNGLFERVHDQLASERRFTADAAHELRTPIAAIRMQAQVAQGATGDDERRAALAAT
                     IAGCDRATRLVEQLLQLARLEADMADTRAPDSPRGQADLAAAAAQQVHELAAQAGRRG
                     QQMALDRPPHAVAVPMSGPLLAVLLRNLIDNALRYGPDGGRVRVQVLGATADAGAHLV
                     AEDAGPGLSDADLQRLGERFFRVLGSGQSGSGLGWSIVRRLARLHGLDVALDRSPTLG
                     GLRVTVRWPRG"
     gene            complement(6274..6930)
                     /locus_tag="PKV30_02440"
     CDS             complement(6274..6930)
                     /locus_tag="PKV30_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRL29110.1"
                     /translation="MRVLLAEDDELLGSGLKAGLGQHGFAVDWVRDGVAAERELLARQ
                     HEVAVLDLGLPRQDGLDALAAVRAKGVRTPVLVLTARDAIDARIQGLDGGADDYLVKP
                     VDLGELAARLRALVRRAHGAPTTRLELGDVALDPAARQVWRGGEPVELSVREFDLLHV
                     FMLNADRVLTRDQLEQHLYKWGSEVSSNAVEVHIHNLRRKLGAELIETVRGVGYVARR
                     "
     gene            complement(7085..7738)
                     /locus_tag="PKV30_02445"
     CDS             complement(7085..7738)
                     /locus_tag="PKV30_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRL29111.1"
                     /translation="MPTPMKIDFVSDVACPWCAVGLASLQQALKNSADAVQATLHFQP
                     FELNPDMPPGGEDVNEHLTRKYGGTPEQFEQNRAAIRARGAAVGFQFNPAGRGRIYNT
                     FNAHRLLHWAGQQGDDQQLALKRALLEAYHGRSEAVEQDDVLLAAVKKAGLDEARARE
                     ILASDEFAAEVRAAERHYQQAGISSVPAVIINDRHLISGGQPPEVFEQALRRLAQAA"
     gene            complement(7786..8682)
                     /locus_tag="PKV30_02450"
     CDS             complement(7786..8682)
                     /locus_tag="PKV30_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29112.1"
                     /translation="MSEVEVPSLLPLAVRARLARARRLTTPCGDGHIVWHAWEPEGAR
                     RAGVAPLVLFHGGSGSWTHWVKNILPLVDAGRWVLAADLPGFGESTPPPGGTDADAMV
                     APLAAGLRALLAGEPVDLVGFSFGGMTAGLLLAEHPALARRLVVVGAPAMGVVPGRQF
                     ELKGWRHLKTPGEQSDIHRHNLGVLMLHDHDLIDGLALEVHIANVLRDRLPRRRLAHT
                     DILARSLPAVTCPVYAIYGEHDAIYRQHIHQLADAYAAAAPDFRGMQLIEGAGHWVQF
                     EAPHAFDAALLRVLEEKKAASA"
     gene            complement(8709..9149)
                     /locus_tag="PKV30_02455"
     CDS             complement(8709..9149)
                     /locus_tag="PKV30_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRL29113.1"
                     /translation="MSSAVALSPTGMRLDKWLWAARFYKTRALAVEQIDKGRVQVNGA
                     DAKPAREVKPGDTVRLRQARDERVVLIKALSPQRGPAPVAQQLYEETPESIAARAQAT
                     EARRLAPEPAHALAQGRPTKRDRRAIDGQRRGDWGDRWSATLDE"
     gene            complement(9146..10540)
                     /locus_tag="PKV30_02460"
     CDS             complement(9146..10540)
                     /locus_tag="PKV30_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HRL29114.1"
                     /translation="MRARSLRRQLLAGILIPMVLIVAFNTWSLYRQALAALNTAYDRT
                     LLASAKSLGESLDVRGEGDAARLQANVPSAALEAFEADLQSRMAYRISTRQGVLLSGY
                     DDLPMWHGQIPMQPPYAALVDFYDGQFRGEPVRMAVLLQPVASTTGRDVAVVQVAETL
                     EIRHALARQILWDTLARQAALIALIAATVIVVVQRATRPVRALSQELQARPDDDLRPI
                     AALDAPRELQPLIAATNEHMRRQSDLLAQQQRFVRDASHQLRTPLAVLKAQVQSARRG
                     DVAPPEALAEIEHTVDRATQLANQMLALAKVAQLQQQADVVPAQFDEVVRAVALDVAP
                     LIAGKGLDFDLQTEPVTLRAHDWMLRELTRNLLHNAIRHTPAGGLLAITLVRAGGEAR
                     LTVADGGPGVAPELAERLFQPFSAGAGGTGSGLGLAICHEIVQTLGGRIQLVNRMHNG
                     RVDGLDAIVSLPTE"
     gene            complement(10537..11217)
                     /locus_tag="PKV30_02465"
     CDS             complement(10537..11217)
                     /locus_tag="PKV30_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL29115.1"
                     /translation="MKLLVVEDDAALQATLVRTLLRRGFGVTAVGDGAAALERWSAAP
                     PDAVVLDLTLPTLDGLQVLAEARARGLRTPVLILTARGTVGDRVLGLNTGADDYLAKP
                     FDLDELEARLRALARRVAPGQPEAPAPAVQIGSIRYDRDGGAIYQGGVPMELTPRELA
                     LMHALLARPGHAVAKERLFEQVFPGEAEVRYEAIEVVAYRLRRKLAGTGLELVTLRGL
                     GYLLRAAT"
     gene            11338..12309
                     /locus_tag="PKV30_02470"
     CDS             11338..12309
                     /locus_tag="PKV30_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29116.1"
                     /translation="MRRDTFLKTLAALAATGALPAAWAQAGANLKILIPANPGGGWDT
                     TGRALGKAMLDAKQASTVTYDNKGGAAGALGLAPFLAGAKGDANQMMVMGAVMLGGLI
                     TSKSPLNLMQATPLARLTTEYNVFVLPANSPHKTMADVVAQLKKDPGSVKWGGGSRGA
                     TEHIAAAMIAREAGVDPAKINYVAFRGGGEATAAILGGNVTVGGSGLSEFSEYIATGK
                     MKPIAVTSAQRLPALKDTPTLKELGLNVEIGNWRGVFGGPGITPAQRKGLIDALVATT
                     KSASWQEALKKNDWTPALLTGDEFTKFVDNEFASLRAIMAKSGMISG"
     gene            12419..12979
                     /locus_tag="PKV30_02475"
     CDS             12419..12979
                     /locus_tag="PKV30_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRL29117.1"
                     /translation="MTSPQPNLPLGLRPDHPEPAHPSRKTLIWETLVSVCLIAVGAAM
                     AYGAVGIPSEAGYGGVGANFLPWVVSIALMVCGAILLWHARTGGYREMEEVEGEESPD
                     WINFVWVSAGLLLNAGLIEHLGFIISCTLAYVLAVQGLRRAYGKPHDTWLVDVITGFL
                     ISAPVYWMFTKFLAINLPGLTSTGWI"
     gene            12984..14483
                     /locus_tag="PKV30_02480"
     CDS             12984..14483
                     /locus_tag="PKV30_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRL29118.1"
                     /translation="MDTFNLLLQGFGTALSLTNLLWALFGCALGTAVGVLPGIGPATT
                     VAMLLPITAKVDITASMIFFAGIYYGAMYGGSTTSILLNTPGETASMVTAMEGNKMAK
                     SGRAGQALATAAIGSFVAGSIATVVVTLFAPVVAEYAVKLGPPEYFLLMVLAFTTVSA
                     VLGKSTVRGLAALFIGLAVGLIGMDQISGQPRYTGGKAEFLDGIEIVLVAVGLFAVAE
                     VLHAVLFEGKIVETQNRLTRVHMDKRDWRRSIPAWLRGTAIGAPFGCIPAGGTEIPTF
                     LSYATEKKLAKGEDRAEFGTKGAIEGVAGPEAANNATVTTALIPLLTLGIPTSNTTAI
                     LLGAFQNYGIQPGPQLFTTSAALVWALVASLYIGNIMLLVLNLPMVGLWVKLLRIPKP
                     QLYAGILIFATVGAYGMRQSSFDLFLLYVIGAIGVVMRRFDFPTAPVVVGMILGPLAE
                     AQLRNAMSIGEGKWSVFVERPVSIFLIVVIVTVLLLPRILRWRAARRAA"
BASE COUNT         2383 a         5244 c         4909 g         2252 t
ORIGIN      
        1 ggccggcacc gcatccagcg ccgtgtacag ccgcttgagc gccgcgcgcg catccgccag
       61 atgcacgtcg ctgtaattga gcgggctgcg gtagtgcgcg cgcacgacga aaaagcggat
      121 cgtctcggcg tcgaacttct ccatcacctc gcggatggtg aagaagttgc ccagcgactt
      181 ggacatcttc tcgttgtcga tgttgacgaa gccgttgtgc atccacacct tggccagcgg
      241 cacgccgtgg gcgccttcgc tttgggcgat ttcgttttcg tggtgcggaa actgcaaatc
      301 ggcaccgccg ccgtggatgt cgaacgtttc gcccagcagc gcgccgccca tggccgagca
      361 ctcgatgtgc cagccggggc ggccgaggcc ataggcgctg tcccacttgg cgccctcggg
      421 ttcggtcggc ttggtggatt tccacagcac gaagtcgagc gggtcttgct tggcaccatc
      481 gaccgccacg cgctcaccgg cttgcagctc gtcgagcgac ttgcccgaca gcttgccgta
      541 gccggcgaac ttgcgcacgg cgtagttcac gtcgccgttg gtcgcctggt aggccaggcc
      601 cttgtccttc agtttgccga cgatgtccag catctgcggc acgtagtcgg tggcgcgcgg
      661 gtcgtggtca ggccgttgca cgcccaaggc gtcaaagtcg cggtacatct cggcaatcat
      721 gcggtcggtg agttgccgga tggtttcgcc gttttccagc gcgcggcgga tgatcttgtc
      781 ctcgatgtcg gtgatgttgc gcacgaaggt gacccggtag ccgctggcgc gcagccaccg
      841 ctgcacgacg tcaaacgcca cggccgaacg cgcatggccg atgtggcaca ggtcgtacac
      901 cgtgatgccg cacacgtaca tgcgcacgtg acccgggtca atgggagaaa agtccaccag
      961 cccacgcgag agcgtgttgt agatgcgcaa ggtcatggaa aaaaagcgag gaaaggccgg
     1021 cgagctgcgg aaatgattcg gatcgacagg ctggcgacga aagccgaggc cggcacgctg
     1081 aaaaccaagt ggcaaggcac ccggccgtgc acggctgcgt ggctacaatg ggccgcagta
     1141 taagaccctg tttggcgccc gccaatgcgc gtctttccca atcgcacggc atgacttttt
     1201 tccgcacttt tttcacacct caggcccgcg cggccggtct ggccgcagcg ctgctgctgg
     1261 ccggcagcgc ggcctgggcc gacgaatacg ccgaagtgca gcgcctgcaa cgcgccggcc
     1321 agaccagcga agcgctggcg cgcgccgaca agtacatcgc cgccaacccg cgcgacccgc
     1381 agatgcgctt cgtcaaggcc ggcgtgctca ccgccgccgg ccaggcgccg caagccgaag
     1441 aattgctggt gcagctgacg cgcgactacc ccgagctgcc cgagccctgg aacaacctgg
     1501 ccgtgctgca cgccagccgc ggccagctcg acaaggccga cgaagcactg caagccgccc
     1561 tgcgcatcaa ccccgcctac gccaccgcgc tcgaaaatct gggcgacgtg cgcgtgcgcc
     1621 aggcgtccga ggcctaccag cgcgcgcgcc agcttgacgg cgccaacgcc cggctcgcgc
     1681 ccaagatcga ggccttgcgc ggcgccctgg ccggcgcggc caagccgggc ggttgaacgc
     1741 ccagccgcct tgggcgggaa cttgccgccc gcccaaaggc accaaacccc tttcacatcg
     1801 cctgcttcag gagttccgcg tcatgaccct ttcaagaaga acactcgccc gcctggccac
     1861 cacgctggcc gccgccggcg cgctgggcac cgccctgccc gcctttgccg ccgacccgcg
     1921 cgtgaagttc gtcaccagcg agggcgactt cgtggtcgag gtctatcccg acaaggcgcc
     1981 caagacggtc gaaaacttcc tgcagtacgt caaggacaag cattacgacg gcaccatctt
     2041 ccaccgcgtc atcaagaact tcatggtgca aggcggcggc tacgacaagg attacaagca
     2101 aaagcccaac cgcccctcga tcaagcacga aggccgcgaa gcgctggcca agggcctgaa
     2161 gaacgacgtg ggcacggtgg ccatggcgcg caccggcgat ccgcattcgg ccacctcgca
     2221 gttcttcatc aacgtggtcg acaacagccg gctcgacccg gtaatcctgc cggcgggtga
     2281 cccggtgacg ttcgagttcc agggccagct gcaaaaagac attccgcgca agaacgtcga
     2341 gaaccacccg gcgctgtacg gctacacggt gttcggcaag gtcgtgagcg gcatggacac
     2401 ggtggaaaaa atccgcaaca cccccaccgg cgccggcggc cccttcccga ccgacgtgcc
     2461 gcaaaagccg gtggtcatcc agtccgccac attggtcaag taattttcag aaagacaaca
     2521 tgcccaaccc ccaagtcgaa ctgcacatca aggaccacgg cgtcatcacg ctggagctgg
     2581 acgaagccaa ggcgcccaag tcggtcgcca actttctggc ctacgtcaag gccggtcact
     2641 acgacaacac ggtgtttcac cgcgtcatca agaacttcat gatccagggc ggcggtttcg
     2701 agcccggcat gaagcaaaaa ccgacccagg cgcccgtcac caacgaggcc gacaacggcc
     2761 tgaagaacaa ccagtacacc gtggcgatgg cgcgcaccag cgatccgcat tcggccacgg
     2821 cgcagttctt catcaacacg gtggacaacg atttcctcaa cttcaaggcc cccactgcgc
     2881 agggctgggg ctatgccgtg atcggcaagg tggtgaaggg cgaaaacgtg gtcgacgcca
     2941 tcaaggccgt gcccaccacg cgcaagggct ttcacgacga tgtgccacgc gatgacgtgg
     3001 tgatcgagaa ggccgtcgtc gtcggcgcat aaccggcccc gccatgacga ccgtgccccg
     3061 gttcgccgcg ctgcccgcgc gtgcgcactg gcgcacggtc gatttcattt cagacctgca
     3121 cctcaagccc gaggagccac tcactttcga cgcctgggcg cgctacctgc gccacacgcc
     3181 ggccgacgcg gtgttcatgc tgggcgatct gttcgaggtg tgggtgggcg acgattcggc
     3241 cctgcccggc agctttgagg cgcagtgcgg cgaggtgctg cgccaggcca gcgcggcgcg
     3301 cgatgtgttc ttgatgcgcg gcaaccgcga ctttctggtc ggcggtgatt ttctggcgca
     3361 atccggcgtg ggcgatctgg ccgaccccac gctgctcacg ttcgacgaac acacctactt
     3421 gctcacgcac ggcgacctgc tgtgcaccga cgacgtggcc taccagcagt ttcgccagca
     3481 ggtgcgcgcg cccgagtggc aaagcgcctt cctggcgcgc cccttgccag agcgccaggc
     3541 cgtggcgcgc cagatgcgcg cgcagagcga ggcgcagcac gccgcgcaag gcgtttatgc
     3601 gcaggtcgat gccgatctgg cccgccgctg gctggccgag gccgatgcca ccgtgctgat
     3661 ccacggccac acgcaccggc cgggcgatca cgccctgggc gccgatgcgc gcggccgacc
     3721 gctgacgcag gtggtgctga gcgactggca cgtcaccgcc accgagcgcc gccttgaagt
     3781 gctgcgcctg acggcccaag gcggcttgca acgcgtgtcc cccggcccca cctgacggcc
     3841 atgctcacaa acctgacgcg ctgggtgcgc cgcgccgccg gtggcccgac gcccatcccc
     3901 gacgaactgt ggcgccagac gatgcggcac cacccgtttc tgcgcaccct gagcacgggc
     3961 gaatacgccc ggctgcggcg cctgtcggag cacttcctcg accgcaagga attcatcggc
     4021 gcacatgggc ttgaagtgac cgacgccatg gccgtcgccg tggctgcgca ggcctgcctg
     4081 ccactgctgc acctgggccc gcccgattcg cccgaagacg cgctgcagtg gtacgacgat
     4141 ttcgtcggca tcgtgatgca ccccgacgcc gccgtggcgc gccgccagcg caccgacgaa
     4201 gccggcgtga tgcaccacta cgacgaggtg ctgtcgggcg aagccatgca cggcggcccg
     4261 gtcatgctga gttggccggc ggtgcaaaac gccggcaccg atcaggatgc cgaaggcagc
     4321 gtggtgatcc atgaattcgt gcacaagatg gacatgcaag gcggcagcgc ggatggcgcg
     4381 cccccgctgc cgcgcggctt catgggcgcg accagcagcc gcgaggcggc ggcgcactgg
     4441 taccgcacct gggcgccggc gtacgagcgc tttcgcgagc aggtcacgct ggcccagcgc
     4501 tttggcgcgc ccgcgccctg gctcgacgcc tatggcgcca ccgcgccggc cgagttcttc
     4561 gccgtcgcct gcgaggcgca cttcgtgcag cccgagcgct ttgcgctgga gtttcctgaa
     4621 ctgagcgccg ccctgcgcgc tttcttcaag caaaaatagg cgccagcgca cgtccagcaa
     4681 gcgcgggcag ctactttttt tgtagtctcc tgttacccac gcggccaacg caccgtcacg
     4741 cgcaggccgc ccagcgtggg gctgcggtcc agcgccacgt ccagcccatg cagccgcgcc
     4801 agccgccgca cgatggacca gcccaggccg ctgccgctct ggccgctacc cagcacgcgg
     4861 aagaagcgct cgcccagccg ctgcaaatcg gcgtccgaca agcccggccc cgcgtcttcg
     4921 gccaccagat gcgccccggc gtcggcagtg gcgcccagca cctgcacccg cacgcggccg
     4981 ccgtccgggc cgtagcgcag cgcgttgtcg atcaggttgc gcagcagcac ggccagcagc
     5041 gggccgctca tcggcaccgc gacggcgtgc ggcggtcgat ccagcgccat ctgctggccg
     5101 cgccgcccgg cctgggccgc caactcgtgc acctgctgcg ccgccgcggc cgccagatcg
     5161 gcctggccgc gcgggctgtc gggcgcgcgg gtatcggcca tgtcggcctc cagccgcgcc
     5221 agttgcagca gttgctcgac caggcgcgtg gcgcgatcgc agccggcaat ggtggcggcc
     5281 agcgcggcgc gccgttcgtc gtcgcccgtg gcgccctggg ccacctgggc ctgcatgcgg
     5341 atggcggcga tcggcgtgcg caactcgtgc gcggcgtcgg cggtgaagcg gcgctcgctg
     5401 gccagttggt cgtgcacgcg ctcgaacagt ccgttcagcg cctgcaccag cggccgcacc
     5461 tcgggcagcg tgtcgtcttc gggcagccgg tccatcgcct gcgggcggcg cgcggcgacg
     5521 ctggcgctca ggcgctcgag cgggcgcacc gcgcgccgca ccgcccacca gatgccggcg
     5581 gccagcaagg gcagcgccgc cagcagcggg atcagcgtgc cgcgcaggct ggccagcagc
     5641 acatggcggc gcgcggaggc gtgctcgccg acctggatga cgtcccgcac ctggccgccc
     5701 tcctcgcgcg ccgtgctgaa cacgcgccag atgcggccgc cgatctgctg gttcgacagg
     5761 ccgcgcacgt ccagcgccgc cagcggctgc tcgggtgcgt cggccgagcg cgcgcgcagc
     5821 ttgccctggc gccagatctg gaacgccacg cgcgactggt atttgtgcag cagcgtcggc
     5881 gcccgcacca cggcgtcgcc atcgtcgtcg tcgacctcgc cggtcgtcag cagcgccgcc
     5941 gtctgcgcca gatgcgcgtc gagcagctcg ttcaactcat gctgcgtcac gaaccacgcc
     6001 accgccgccg tcagcaccca cgtgaccaac acgaagccca ggaccatcgc catcagccgc
     6061 tgggccagcg accaggtgcg gcgcggcgca gtgctcatat caccacccag ccccgctgaa
     6121 acccgcccgc atccgacaac acaagaaaga gcccaacggc cgcggagcag gccatgcgcg
     6181 ggcactcgcg gaagggcttg gcccgcccgc tgggtgcgtc ccccttcccg aagagagaag
     6241 gggaaaggcg ccgcaggcga ctcaggggga tgctcatctt ctagccacgt agcccacgcc
     6301 acgcaccgtc tcgatcaact cggcacccag cttgcgccgc aggttgtgga tgtgcacttc
     6361 caccgcgttg ctgctgactt cgctgcccca cttgtacagg tgctgctcca gctggtcgcg
     6421 cgtcagcacg cggtcggcat tcagcatgaa gacgtgcagc agatcgaatt cgcgcaccga
     6481 cagctccacc ggctcgccgc cgcgccacac ctggcgcgcg gcggggtcga gcgccacgtc
     6541 gcccagctcg agccgcgtgg tgggcgcacc gtgcgcacgg cgcaccagcg cccgcaggcg
     6601 tgcggccagc tcgcccaggt caacgggttt gaccaggtaa tcgtcggccc cgccgtccag
     6661 cccctggatg cgcgcgtcga tggcgtcgcg cgccgtcagc accagcaccg gcgtgcgcac
     6721 gcccttggcg cgcacggcgg ccagggcatc cagaccgtct tgacgaggca ggccaagatc
     6781 gagcaccgcc acctcgtgct gtcgcgccag cagctcgcgc tcggcggcga cgccatcgcg
     6841 cacccagtcg accgcaaagc cgtgctggcc caagccggcc ttcaaaccgg agccgagcag
     6901 ctcgtcgtct tcagccagca gcacacgcat gaagggcttc ggcgatcgtc atgcggcgat
     6961 tgtgcggcgt gctggccggg catgacaccg cgctgaatcc ccgctactga ttgctacgct
     7021 aaccatagct gtttgcgctt gtccatcaag cgccggtggg caaaatggct tgaattttcc
     7081 gccatcacgc ggcttgcgcc agccgccgca gcgcctgctc gaacacctcg ggcggctggc
     7141 cgccgctgat caagtggcgg tcgttgatga tgaccgccgg caccgacgag atgccggcct
     7201 gttggtagtg gcgctcggcc gcgcgcacct cggcggcgaa ttcgtcgctg gccagaattt
     7261 ctcgcgcgcg tgcttcgtcc agacccgctt tcttgactgc ggccagcagc acgtcgtctt
     7321 gctccaccgc ctcgcttcga ccgtggtacg cctccagcag cgcgcgcttc aaggcgagtt
     7381 gctggtcatc gccctgctgc ccggcccagt gcagcaggcg gtgcgcgttg aaggtgttgt
     7441 agatgcgccc gcgcccggcg gggttgaact ggaagccgac cgccgcgccg cgcgcgcgga
     7501 tggcggcgcg gttttgctcg aactgctcag gcgtgccacc gtacttgcgc gtcaggtgct
     7561 cgttcacgtc ttcaccgccg ggcggcatgt cggggttcaa ctcaaagggc tgaaagtgca
     7621 gcgtggcctg caccgcatcg gcgctgttct tcaaggcctg ctgcagcgac gcgaggccca
     7681 cggcgcacca cgggcaggcg acgtcggaga caaagtcgat cttcatcggt gtgggcatgg
     7741 tgatttcctt atgcttccaa aataatagct tatcgcgctt gtccatcaag cgctggcggc
     7801 ctttttctct tcaagaacgc gcagcaacgc ggcgtcaaag gcatgcggcg cctcgaactg
     7861 cacccagtgg cccgcacctt caatcaactg catgccccga aaatccggcg ccgccgccgc
     7921 gtaggcatcg gccagctggt ggatgtgctg gcggtagatc gcgtcgtgct cgccgtagat
     7981 ggcgtacacc gggcaggtca cggcgggcaa gctgcgcgcc agtatgtcgg tgtgcgccag
     8041 ccgccggcgc ggcaggcggt cgcgcagcac gttggcgatg tgcacttcca gcgccaggcc
     8101 gtcgatcagg tcgtggtcgt gcagcatcag cacgcccagg ttgtggcggt ggatgtcgga
     8161 ctgctcaccg ggcgtcttca ggtggcgcca gcctttcagc tcgaactgcc gcccgggcac
     8221 cacgcccatg gccggcgcgc cgaccaccac caggcgccgc gccagcgccg ggtgttcggc
     8281 cagcagcagg ccggccgtca tgccgccaaa cgagaagccg accagatcga ccggctcgcc
     8341 cgccagcagc gcccgcaggc cggcggccag cggggcgacc atggcatcgg cgtcggtgcc
     8401 gcccggcggc ggggtcgact cgccaaagcc cggcaaatcc gccgccagca cccagcggcc
     8461 ggcatcgacc agcggcagga tgtttttcac ccagtgcgtc cagctgccgc tgccgccatg
     8521 aaacagcacc agcggcgcca cgccggcgcg gcgcgcgcct tcgggctccc aggcatgcca
     8581 gacgatgtgg ccgtcgccgc acggcgtggt caggcggcgc gcccgggcca gccgcgcgcg
     8641 caccgccagc ggcaacaggc tggggacttc gacttcgctc atgcggtcat tgtgctttgg
     8701 ggcggcgctc actcgtccag cgtcgccgac cagcggtcgc cccagtcgcc gcggcgctgg
     8761 ccgtcgatgg cgcggcggtc gcgcttggtg gggcggccct gcgccagcgc gtgcgcgggt
     8821 tcgggcgcca gccggcgcgc ctctgtggct tgtgcgcgcg cggcgatgct ctcgggcgtt
     8881 tcctcgtaca actgctgcgc caccggcgcc gggccgcgct gcggcgacaa ggccttgatc
     8941 agcaccacgc gctcgtcgcg cgcctggcgc aggcgcaccg tgtcgccggg cttgacctcg
     9001 cgcgccggct tggcgtcggc gccgttgacc tgcacgcggc ccttgtcgat ctgctcgacg
     9061 gccagcgcgc gcgtcttgta aaagcgcgcg gcccagagcc atttgtccag gcgcatgccg
     9121 gtgggggaaa gagcgactgc ggaactcatt cagtgggcaa gctgacgatg gcatccaggc
     9181 catccacgcg gccattgtgc atgcgattga cgagttggat gcgcccgccc agcgtctgca
     9241 cgatctcgtg gcaaatggcc aggccgagcc ccgaaccggt gccgccggca cccgcgctga
     9301 aaggctgaaa aaggcgctcg gccagctcgg gcgcgacgcc agggccgccg tcggccaccg
     9361 tcaggcgcgc ctcgccgccc gcgcgcacca gcgtgatggc cagcaggccg ccggccggcg
     9421 tgtggcgaat ggcgttgtgc agcaggttgc gcgtgagttc gcgcagcatc cagtcgtgcg
     9481 cgcgcagcgt gaccggctcg gtctgcaggt cgaagtccag ccccttgccc gcgatcagcg
     9541 gcgccacatc cagcgccacg gcgcgcacca cctcgtcgaa ctgcgctggc accacatccg
     9601 cctgctgctg cagctgcgcc accttggcca gcgccagcat ctggttggcc agttgggtgg
     9661 cgcggtcgac cgtgtgctcg atctcggcca gcgcctcggg cggcgccacg tcgccgcgcc
     9721 gcgccgactg cacctgcgcc ttcagcaccg ccagcggcgt gcgcaactgg tgcgatgcgt
     9781 cgcgcacaaa gcgctgctgc tgcgccagca gatcgctctg gcggcgcatg tgttcgttgg
     9841 tggcggcaat cagcggctgc agctcgcgcg gggcgtcgag cgcagcgatg gggcgcaggt
     9901 cgtcgtccgg ccgcgcctgc agctcctggc tcagcgcgcg caccggccgc gtggcgcgtt
     9961 gcaccaccac gatgacggtg gcggcaatca acgcgatcag tgccgcctgg cgcgccagcg
    10021 tgtcccacag gatctggcgc gccagcgcat ggcggatttc cagcgtctcg gccacctgca
    10081 ccacggcgac gtcgcgcccg gtggtgctgg ccaccggctg cagcagcacc gccatgcgca
    10141 ccggctcgcc gcgaaactgg ccgtcgtaaa aatccaccag cgcggcgtac ggcggctgca
    10201 tcggaatctg cccgtgccac atcggcaaat cgtcgtagcc tgacagcagc acgccctgcc
    10261 gggtcgagat gcggtaggcc atgcggcttt gcagatcggc ctcgaacgcc tccagcgcgg
    10321 ccgagggcac gttggcctgc aggcgcgccg cgtcgccctc gccgcgcacg tcgagcgatt
    10381 cgcccaggct cttggccgag gccagcagcg tgcggtcgta cgcggtgttg agcgcggcca
    10441 gcgcctggcg gtacaggctc caggtgttga aggcaacgat cagcaccatg gggatcagga
    10501 tgccggccag caactggcgc cgcagcgagc gcgccctcat gtcgctgccc gcagcagata
    10561 gcccagcccg cgcagcgtga ccagttccag ccccgtgccg gccagcttgc ggcgcaggcg
    10621 gtaggccacc acctcgatgg cttcgtaacg cacctcggcc tcgccgggaa acacctgctc
    10681 gaacagccgc tccttggcca ccgcgtggcc ggggcgcgcc agcagcgcgt gcatcagcgc
    10741 cagctcgcgc ggggtcagct ccatcggcac gccgccctgg taaatggcgc cgccgtcgcg
    10801 gtcgtagcgg atgctgccga tctgcacggc gggcgcgggc gcctcgggtt gccccggggc
    10861 cacgcgccgg gccagcgcgc gcaggcgcgc ttccagctcg tccagatcga agggcttggc
    10921 caggtagtcg tcggcgccgg tgttcaggcc cagcacgcgg tcgcccacgg tgccgcgcgc
    10981 cgtcaggatc agcaccggcg tgcgcaagcc gcgcgcgcgg gcttcggcca gcacctgcag
    11041 accgtccagc gtgggcagcg tcaggtcaag caccacggca tcgggcggcg cggccgacca
    11101 gcgctccagc gccgccgcac cgtcgcccac ggcggtgacg ccaaaaccgc gccgcagcag
    11161 cgtgcgcacc agcgtcgcct gcagcgcggc atcgtcttca accaccagca gtttcatgcg
    11221 cggcaacata ccgcagtggt gccggctaag ggtaagccct aggccgctgg acagccaact
    11281 gacagccgct cgcgccatca tgcgggcgtt tcattccaaa tgcggagaca agccaccatg
    11341 cgacgcgaca ccttcctgaa aaccctggcc gcgctggccg ccacgggcgc gctgcccgcc
    11401 gcctgggcgc aggccggcgc caacctgaag atcctgatcc ccgccaaccc cggcggcggc
    11461 tgggacacca ccggccgcgc cctgggcaaa gccatgctcg acgccaagca ggccagcacc
    11521 gtcacctacg acaacaaggg cggcgcggcc ggcgccttgg gcctggcgcc cttcctggcc
    11581 ggtgccaagg gcgatgccaa ccagatgatg gtgatgggcg cggtgatgct gggcgggctg
    11641 atcacctcca agtcgccgct caacctgatg caggccacgc cgctggcgcg cctgacgacc
    11701 gaatacaacg tgttcgtgct gccggccaat tcgccccaca agacgatggc cgacgtggtc
    11761 gcgcagctca aaaaagaccc cggcagcgtg aagtggggcg gcggctcgcg cggtgccacc
    11821 gagcacattg cggcggccat gattgcgcgc gaagcgggcg tcgacccggc caagatcaac
    11881 tacgttgcct tccgtggcgg cggcgaggcg acggcggcca tcctgggcgg caacgtcacg
    11941 gtgggcggca gcggcctgtc ggagttttcg gaatacatcg ccaccggcaa gatgaagccg
    12001 attgccgtca cctcggcgca gcgcctaccg gcgctgaagg acacgccgac gctgaaggag
    12061 ctggggctga acgtcgagat tggcaactgg cgcggcgtgt ttggcggccc cggcatcacg
    12121 ccggctcagc gcaagggctt gatcgacgcg ctggtggcca ccaccaagtc ggcctcgtgg
    12181 caagaggcgc tgaagaagaa cgactggacg ccggcgctgc tgacgggcga cgagttcacc
    12241 aagttcgtcg acaacgagtt cgccagcctg cgcgccatca tggccaagtc gggcatgatc
    12301 agcggttaac aacaaaacag gccgccagcg cttgtccagc aagcgcgagt agctataaat
    12361 aaaagagtat tccggtccgc gtcccgcgca gggcgtggcc ggctgcagga gacagcgcat
    12421 gaccagcccg caacccaatt tgccgcttgg cctgcggccc gaccaccccg agccggcgca
    12481 cccgtcgcgc aagacgctga tctgggagac gctggtcagc gtctgcctga tcgccgtcgg
    12541 cgccgccatg gcctatggcg ccgtcggcat tccgtccgaa gccggctacg gcggcgtcgg
    12601 cgccaacttc ttgccgtggg tggtgtcgat cgcgctcatg gtgtgcggcg ccatcctgct
    12661 gtggcacgcg cgcaccggcg gctaccgcga gatggaggaa gtcgagggcg aggaaagccc
    12721 ggactggatc aacttcgtct gggtgtcggc cggcctgctg ctgaacgccg ggctgatcga
    12781 gcacctgggc ttcatcatca gctgcacgct ggcctacgtg ctggcggtgc agggcctgcg
    12841 ccgcgcctac ggcaagccgc acgacacgtg gctggtcgac gtcatcacgg gctttttgat
    12901 ctcggcgccc gtgtactgga tgttcaccaa gttcctggcg atcaacctgc cgggcctcac
    12961 atcgaccggg tggatctgaa gccatggaca ccttcaacct cctgctgcag ggctttggca
    13021 ctgcgctgtc gctcaccaac ctgctgtggg cgctgttcgg ctgcgccttg ggcaccgccg
    13081 tcggcgtgct gcccggcatc ggccccgcca ccacggtggc gatgttgctg ccgatcaccg
    13141 ccaaggtcga catcaccgcg tcgatgatct tttttgccgg catctattac ggcgcgatgt
    13201 atggcggcag cacgacgtcg atcctgctca acacgccggg cgaaaccgcc agcatggtca
    13261 cggccatgga aggcaacaag atggccaaga gcgggcgcgc cggccaggcg ctggccaccg
    13321 cggcgattgg ctccttcgtg gccggcagca tcgccaccgt ggtggtcacg ctgtttgcgc
    13381 cggtggtggc cgaatacgcg gtcaagctcg gcccgcccga atacttcctg ctcatggtgc
    13441 tggccttcac caccgtgagc gcggtgctgg gcaagagcac ggtgcgcggg ctggcggcgc
    13501 tgttcatcgg cctggccgtg ggcctgatcg gcatggacca gatcagcggc cagccgcgct
    13561 acaccggcgg caaggccgag tttctggacg gcatcgagat cgtgctggtg gccgtgggcc
    13621 tgtttgccgt ggccgaggtg ctgcacgccg tgctgttcga gggcaagatc gtcgagacgc
    13681 aaaaccgcct gacgcgcgtg cacatggaca agcgcgactg gcgccgcagc atcccggcgt
    13741 ggctgcgcgg cacggcaatt ggcgccccgt ttggctgcat tcccgcgggg ggcaccgaga
    13801 tcccgacctt cctgagctac gccaccgaga aaaagctggc caagggcgaa gaccgggccg
    13861 aattcggcac caagggcgcc atcgaaggcg tggccggccc cgaggccgcc aacaacgcca
    13921 cggtgacgac ggcgctcatc ccgctcttga cgctgggcat cccgaccagc aacaccacgg
    13981 cgatcttgct gggcgcgttc cagaactacg gcatccagcc cggaccgcag ctgttcacca
    14041 cctcggccgc gctggtgtgg gcgctggtgg cgtcgctgta catcggcaac atcatgctgc
    14101 tggtgctgaa cctgccgatg gtcggcctgt gggtcaagct gctgcgcatc cccaagccgc
    14161 agctgtacgc cggcatcctg atcttcgcca ccgtgggcgc gtacggcatg cggcagagca
    14221 gcttcgacct gtttttgctc tacgtcatcg gcgccatcgg cgtggtgatg cggcgctttg
    14281 actttcccac cgcgccggtg gtggtcggca tgatcctggg gcccttggct gaagcgcaac
    14341 tgcgcaacgc gatgtcgatt ggtgaaggca agtggagcgt gtttgtggag cgcccggtgt
    14401 cgatcttcct gatcgtggtg atcgtcaccg tgctcctgct gccgcgcata ttgcgctggc
    14461 gtgcggcgcg gcgcgctgcg tgatcagcgc tctacaaaaa aaccgaacag ccggacgcag
    14521 aggacgcaaa agaacagcca aaaaggtttt ctttgaatct tttgcgtcct ctgcgaaatc
    14581 tctgtgttct ctgcgtccgg tttttaggct tcaaagtgcc tccagcgctt gcccatcaag
    14641 cgcgagcagc tatcatttaa gtagcgtttg tctcgcttca caccagccgc tcaatcgtct
    14701 tgtgccgcca caccaggcgg tagaaggcgc tttgcagcag cagcatggtg acaaacacca
    14761 ccggataggc cagccacacg ccgtccag
//
LOCUS       DAOMVO010000024        14776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_866_length_14776_cov_13.509001, whole genome shotgun sequence.
ACCESSION   DAOMVO010000024 DAOMVO010000000
VERSION     DAOMVO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14776
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_866_length_14776_cov_13.509001"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKV30_02485"
     CDS             <1..219
                     /locus_tag="PKV30_02485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HRL29119.1"
                     /translation="NYTDPLPQALVLTAIVIGFATSALFLVVLLASRGLSDTDHVDGE
                     EPDDGTGHVSIGSAAAAPPGESEEGRQA"
     gene            216..1922
                     /locus_tag="PKV30_02490"
     CDS             216..1922
                     /locus_tag="PKV30_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HRL29120.1"
                     /translation="MTFARLVEGLMPHLIVVPILLPMVTAAVMLLLGDTRRPLKAIIN
                     VLSCVLGVGVAIALIYWVQRLGGPQAVGVYLPGNWPVPYGIVLVVDRLSAMMLLLTSV
                     LGLGGVLYASARWHKAGVHFHPLFQLQLMGLYGAFLTADLFNLFVFFEIMLTASYGLL
                     LHGSGWARVRSGLHYIAMNLMASSMFLIGVAMLYGVTGTLNMADMAIMVPAIPENDRG
                     LLHAGAAILAIAFLVKAAAWPLNFWLPMAYSSAAAPVAALFAIMTKLGVYAILRVWTL
                     LFPQDAGVSALFGGPVLVGLGLATLTFGIIGVIASQHLNRMVAFCAIVSSGTLLAALG
                     FGQPAVTGGALYYTLGSTLAVSAFFLLTELIDRTREVEENPIYVSEVEPDESFAYPVD
                     LVPVRDVNLDDDEEALFGRAIPAAMAFLGLAFIVCALTIAGLPPMAGFVGKVVMLSAL
                     LNPGGFGEQAPVSLAAWALLALLVVGGLLTTIAMSRAGVRYFWAPQGRAVPRLRIIET
                     VPIAMLLLLIGALVWQADAVLRYANATARSLHDPREYIGSVVSTKPLPSPHTTGEGGL
                     RR"
     gene            1919..2410
                     /locus_tag="PKV30_02495"
     CDS             1919..2410
                     /locus_tag="PKV30_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRL29121.1"
                     /translation="MKRLLPAPLLTAALICMWLVLNRSLSLGQILLAVLFGVAAPALL
                     APLRPARPRIRHPLVVLRLIGVVGVDVIRSSLDVFWTLVKSRVQPPNSRFVVIPLDLR
                     DPTALAALAMITTVVPGTVWCELARDGSSLLLHVWDAPDTEAFIEHYKRAYERPLMEI
                     FES"
     gene            2407..2685
                     /locus_tag="PKV30_02500"
     CDS             2407..2685
                     /locus_tag="PKV30_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HRL29122.1"
                     /translation="MSPLLSGAITLTLWLYGLAILLMLWRMLRGPSAQDRVLAIDAIY
                     VIGMLVALVVGIRYTSSMYFEAALLIALFGFVSTAAMAKFLLRGEVIE"
     assembly_gap    2838..2937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3170..3568
                     /locus_tag="PKV30_02505"
     CDS             3170..3568
                     /locus_tag="PKV30_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HRL29123.1"
                     /translation="MTPEPLPLWIEAIVAALLVCSAVFVLAAAWGLVRLKDFFQRMHP
                     PALVFVWGSWCVSLASIIYFSALAHGPELHVWLIIILLSITVPITTALLARAALFRGR
                     QAGQQLPPALQPRNASPASAPPPTRSSEEK"
     gene            complement(3641..4921)
                     /locus_tag="PKV30_02510"
     CDS             complement(3641..4921)
                     /locus_tag="PKV30_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL29124.1"
                     /translation="MASSVRRALLRSLLAGLATASLGARAQGADAGPVKLALIESLSG
                     PFANTGEAVYRNLAWAIERVNARGGVATARGRRLLQLDRFDNKGRVEESLSMLRAAVD
                     GGAQFVLQGNSSSVAAALIDAINKHNQRAPEQRVVFLNYSAVDPILTNELCSFWHFRF
                     DAHADMRMAALMQLIQHDAQLKGMYLIGQDYSFGQAVLREARRQLAEQRPDVAIVGEE
                     LHPIGRVKDFMPYAAKIKASGAQAVLTGNWGNDLTLLVKAARDAGFGGRFYTFYGNAL
                     GAPAAIGEAGVGKVVAVADWLPNVPTPESAAFYRSFRERFPRPQEDYVHMRMQLMIEA
                     LAQAIERAGGSDPLQVARQLERADVTLAGQRGRMRAADHQFQQQLAVGVMERQGTPGV
                     PFDVEGSGYGFRVVRQIEPDSAAMPHSCKMARPG"
     gene            complement(4991..5551)
                     /locus_tag="PKV30_02515"
     CDS             complement(4991..5551)
                     /locus_tag="PKV30_02515"
                     /inference="COORDINATES: protein motif:HMM:NF021515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HRL29125.1"
                     /translation="MSRRLLPLAASLLLAGCAGLASSPVPLGDYQLEAFNADTFVRHY
                     VAPPVRTCEAARRALLSQGYIVTDATADKVIARKYFQPRAEHHVQLEFRVVCARESGD
                     ADATVAFASGLKEQYVVRKVKESASLGVGGIGSLSLPVEGSLDSMVKVSSETVDDATL
                     YQRFFELLGQYLDRSVAPEAVPAAAS"
     gene            5782..6159
                     /locus_tag="PKV30_02520"
     CDS             5782..6159
                     /locus_tag="PKV30_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRL29126.1"
                     /translation="MIFTATTPASRRSVYGPALRSLDRFLDDALTVARQGATQFKDEG
                     AHWSLSIDVPGVAREQLSIAIEGQTVKIETVEGAPRQYKKVYEFEQEIDAAASQAKLE
                     LGVLTLTLAKLQPASKATTLPIN"
     gene            complement(6316..7362)
                     /gene="tsaD"
                     /locus_tag="PKV30_02525"
     CDS             complement(6316..7362)
                     /gene="tsaD"
                     /locus_tag="PKV30_02525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRL29127.1"
                     /translation="MLVLGIESSCDETGVALVRTREGAVPELLGQALHSQIAMHADYG
                     GVVPELASRDHIRRIIPLANQALAQAGQALAAIDVVAYTRGPGLAGALLVGAGVACAL
                     GAALRKPVLGVHHLEGHLLSPFLSADPPEFPFVALLVSGGHTQLMRVDGVGQYELLGE
                     TIDDAAGEAFDKSAKLLGLGYPGGPALARLADFGDATSFKLPRPLLHSGDLDFSFAGL
                     KTAVLTQARKFEGSPCEQQRADLAASTQAAIVEVLVKKSLKALAQTGLNRLVVAGGVG
                     ANAALREQLNAACAARGARVHYPELALCTDNGAMIALAAAMRLQAGVEAPNRDYAFDV
                     KPRWPLDQLAALEN"
     gene            complement(7408..7848)
                     /locus_tag="PKV30_02530"
     CDS             complement(7408..7848)
                     /locus_tag="PKV30_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRL29128.1"
                     /translation="MPTVADILSKRDARSLASLSPTATVRDALQLMADRNIGSVLIMQ
                     GEALLGIFTERDYARKLALKGLASADTPLADVMTAKLYVVSPRQTVQECMGIMTQAKI
                     RHLPVVSDGAVQGLVSIGDLVNATMAEQEFLISQLGSYISGDLT"
     gene            8104..8799
                     /locus_tag="PKV30_02535"
     CDS             8104..8799
                     /locus_tag="PKV30_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRL29129.1"
                     /translation="MNPTPLAVISSMATKALLTELVAEFQRLHPGRAVALESVGGVDA
                     ARRVAAGEALDAVVLGSDAIDQLIASGHVRAGSRVDIVRSGVAIAVRAGAPRPDTGSE
                     AALKAAVLAARSVGYSTGPSGVALARLFERWGIADQVRAKLVTPPPGTPVGALIASGE
                     VELGFQQLSELMHLPGIDVIGPLPPEMQIETVFAGGVAATSTQTDAARELLAFLASAP
                     LAAAKQKHGMAPA"
     gene            complement(8856..9221)
                     /locus_tag="PKV30_02540"
     CDS             complement(8856..9221)
                     /locus_tag="PKV30_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRL29130.1"
                     /translation="MAKKLYVGNLAYSVRDEDLQQMFAEHGNVSSASVAMERDSGRSR
                     GFGFVEMANDAEALAAIEALNGQSQGGRALVVNEARPREERSGGGGGYGGGGGGGYGG
                     GGRSGGGGGGGYRGGGNRY"
     gene            complement(9278..9541)
                     /gene="infA"
                     /locus_tag="PKV30_02545"
     CDS             complement(9278..9541)
                     /gene="infA"
                     /locus_tag="PKV30_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRL29131.1"
                     /translation="MSKEDLIEMRGRVDEVLPDARYRVTLDNGHQLVAYSAGRMRKHR
                     IRILAGDFVSLELSPYDLGKGRITFRHLAGQAPRGPRPGVRRH"
     gene            complement(9549..9773)
                     /locus_tag="PKV30_02550"
     CDS             complement(9549..9773)
                     /locus_tag="PKV30_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29132.1"
                     /translation="MSNPCFSLGRYVVSPLTRLLDDGAYSASVSIRSGRGQASSDRVL
                     RLIGRFTTADGALRHAAAQGRAWVHAACGA"
     gene            10165..10494
                     /locus_tag="PKV30_02555"
     CDS             10165..10494
                     /locus_tag="PKV30_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRL29133.1"
                     /translation="MSLAPTDPDVRALLAGTLAQTRSSAAVICLCAAWCGTCREYAPL
                     FEQVAAAHPHLAFRWVDIEDEADALGDLDIDTFPTLVVGAGAVQFAGPVLPQAGQIER
                     LLKSLGL"
     gene            10585..11532
                     /gene="ybiB"
                     /locus_tag="PKV30_02560"
     CDS             10585..11532
                     /gene="ybiB"
                     /locus_tag="PKV30_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HRL29134.1"
                     /translation="MGISPYLKEIGRGSAGARHLTREQAADLMGQLLDGQCTDLELGA
                     FCIAMRVKGETPAEMAGFLDAVDTRITKLPASTRPVVVLPSYNGARKLPVLTPLLALL
                     LARKGLPVLIHGAATEDRRVSTQEVLSALGLSAQAAIQSIATGQVHFVATDSLHPGLK
                     RLLDVRRTIGLRNSAHSLVKLMNPVAGTALVVGSYTHPEYAESMTATFRLMGTDAMLL
                     RGTEGEPVADPRRTPRMDVFLRGEIDTVQAMREGPLASLPSLPTQIDAASTAHYIRAV
                     LEGLIPLPEPIGIQADHIVRACKALEAVGDSSCLQALAA"
     gene            11538..12314
                     /gene="cobA"
                     /locus_tag="PKV30_02565"
     CDS             11538..12314
                     /gene="cobA"
                     /locus_tag="PKV30_02565"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRL29135.1"
                     /translation="MNTPLGQVTLVGAGPGDPELLTLKAARAIQAASVILVDDLVGEG
                     VLAHARPEARITYVGKRGGCKSTPQATIEQLMLDAARAGENVVRLKGGDPFIFGRGGE
                     EVESLRRAGIEPVVINGITSGLAAITSLGAPLTHREHAQGVVFVTGHPQKDGPGLDWV
                     RLGESARDLRLTLVIYMGISSAARIQSGLLAALPGDTPVAVIERATLKNQRQCLTSLR
                     ELLSGLQQSGLGSPAIMVVGDVVTGVREALRAPALADQSA"
     gene            complement(12302..12433)
                     /locus_tag="PKV30_02570"
     CDS             complement(12302..12433)
                     /locus_tag="PKV30_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29136.1"
                     /translation="MTAFLAAGVRADLPDARGRTVHDCARSDPVRALLAGAAPRQAD"
     gene            complement(12582..12998)
                     /locus_tag="PKV30_02575"
     CDS             complement(12582..12998)
                     /locus_tag="PKV30_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRL29137.1"
                     /translation="MGFENLLEAIVDDDTQRVAALLAADARLAQASVAQARLYEEGIF
                     HWLYAGDTALHLAAAGYRTSIVEQWLAAGADVNAQANHRRSTPLHYAADGFITHPACD
                     AVAQVATLRCLLAAGADLHAQDRNGATALHRAVRTR"
     gene            complement(13016..13363)
                     /locus_tag="PKV30_02580"
     CDS             complement(13016..13363)
                     /locus_tag="PKV30_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRL29138.1"
                     /translation="MAIFLKRAYDPPTASDGKRILVDRLWPRGLAKVKAKFDVWLKDV
                     APSTELREWFGHDPAKWTEFQTRYRAELKGNPAFDELKKLAHQGNITLIYAARDQVHN
                     EAVVLQQLLEHGH"
     gene            13539..14084
                     /locus_tag="PKV30_02585"
     CDS             13539..14084
                     /locus_tag="PKV30_02585"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HRL29139.1"
                     /translation="MQSNRTATNALIALSLGLMAPAAFADDYPPRKAGLWQMTTKSST
                     GGAPEVVHQCIDAASDKAMQQAGKAMAQQMCQDVKLRRDGDKVIIESTCAMSPKSKMV
                     SRGVMSGDYQSAYREESETRFDPPMAGIQNVRTTTEARWVGACKAGQRPGDVVTADGM
                     KMNLLDMVKAGPAMRGKAPAR"
     gene            complement(14141..>14776)
                     /locus_tag="PKV30_02590"
     CDS             complement(14141..>14776)
                     /locus_tag="PKV30_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HRL29140.1"
                     /translation="DMCFELAAAIMSRLEDAATVVDEVHGFKFFDERDLLGFVDGTEN
                     PSAQAAVQATLIGDEDPGFTGGSYVIIQKYLHNLKAWNALPVERQETIIGRKKPSDIE
                     QRPFDKASYAHKVLTNIEEGGQQLQILRDNMPFGQVGKGEFGTYFIGYARSPDRIEKM
                     LNNMFIGNPPGNYDRILDFSTAVTGGLFFVPGVVSGRCGSAGREGVNFRKL"
BASE COUNT         2373 a         5085 c         4884 g         2334 t
ORIGIN      
        1 aactacaccg atccgctgcc gcaggcgctg gtgctgaccg ccatcgtgat cggctttgcc
       61 acctcggcgc tgttcctggt ggtgctgctg gcctcgcgcg gcctgtcgga caccgaccac
      121 gtggatggcg aggagccgga cgatggcacc ggccacgtgt ccatcggcag tgccgccgcc
      181 gcgccgcccg gcgagagcga agaagggcgc caggcatgac atttgcccgc ctcgtcgaag
      241 ggttgatgcc gcacctgatc gtggtgccca tcctgctgcc catggtgacc gccgccgtca
      301 tgctgctgct gggcgacacg cgccggccgc tgaaggcgat catcaacgtg ctgtcttgcg
      361 tgttgggcgt gggcgtcgcc atcgccttga tctactgggt gcagcgcctg ggcgggccgc
      421 aagcggtcgg cgtgtacctg cccggcaact ggccagtgcc ctacggcatc gtgctggtcg
      481 tcgacaggct gtcggccatg atgctgctgc tgaccagcgt gctgggcctg ggtggcgtgc
      541 tgtacgccag cgcgcgctgg cacaaggcgg gggtgcactt tcacccgctg ttccaactgc
      601 agctgatggg cctgtacggc gcgtttctca cggccgacct gttcaacctg ttcgtgttct
      661 tcgagatcat gctgacggcc tcgtacgggc tgttgctgca tggctcgggc tgggcgcgcg
      721 tgcgctcggg cctgcactac atcgccatga acctgatggc gtccagcatg ttcctgatcg
      781 gcgtggccat gctgtacggc gtgaccggca cgctgaacat ggccgacatg gccatcatgg
      841 tgcccgccat ccccgaaaac gaccgcggcc tgctgcacgc cggcgccgcc atcctggcga
      901 tcgcgttttt ggtcaaggcc gccgcctggc cgctcaactt ctggctgccg atggcgtatt
      961 caagcgccgc cgcgccggtg gcggcgctgt tcgccatcat gaccaagctg ggcgtctacg
     1021 ccatcctgcg cgtgtggacg ctgctgtttc cgcaggacgc gggcgtgtcg gcgctgtttg
     1081 gcggcccggt gctggtgggg ctggggctgg cgacgctcac cttcggcatc atcggcgtca
     1141 tcgccagcca gcacctgaac cgcatggtgg cgttttgcgc catcgtgtcg tcgggcacat
     1201 tgctggcggc gctgggcttc ggtcagcctg cggtgaccgg gggcgcgctc tactacacgc
     1261 tgggctcgac cctggcggta agcgcctttt tcctgctgac cgaactcatc gaccgcacgc
     1321 gcgaggtcga ggaaaacccg atctacgtca gcgaagtcga gccggacgaa agctttgcct
     1381 acccggtcga tctggtgccg gtgcgcgacg tcaacctgga cgacgacgaa gaagccctgt
     1441 tcggccgggc catcccggcg gccatggcgt ttctgggcct ggccttcatc gtctgcgcgc
     1501 tgacaatcgc cggcctgccg cccatggcgg gattcgtcgg caaggtggtc atgctgtcgg
     1561 cgctgctcaa cccgggtggc tttggcgaac aggcgccggt gtcgctggcg gcctgggcgc
     1621 tgctggcgct gctggtggtg ggcgggttgc tgaccaccat cgccatgtcg cgcgccgggg
     1681 tgcgctactt ctgggcgccg cagggtcgcg cggtgccgcg cctgcgcatc atcgagaccg
     1741 tgcccatcgc catgctgctg ctgctcatcg gcgcgctggt gtggcaggcc gacgccgtgc
     1801 tgcgctacgc caacgccacc gcgcgcagcc tgcacgaccc gcgcgaatac atcggcagcg
     1861 tggtgtcgac caagcccctg ccctcgcccc acaccaccgg cgaaggaggc ctgcgccgat
     1921 gaagcgcctg ctgcccgcac cgctgctgac ggcggccctg atctgcatgt ggctggtgct
     1981 caaccgttcg ctcagcctgg ggcagatcct gctggcggtg ttgtttggcg tcgccgcgcc
     2041 ggcgttgctg gcgccgctgc gcccggcgcg cccgcgcatc cgccacccgc tggtggtgct
     2101 gcggctgatc ggcgtggtgg gggtggacgt gatccgctcc agcctggacg tgttctggac
     2161 cctcgtcaag agtcgcgtgc agccgccgaa cagccgcttc gtcgtcatcc cgctcgacct
     2221 gcgcgacccc accgccttgg ccgcgctggc catgatcacc accgtggtgc cgggcaccgt
     2281 gtggtgcgaa ctggcgcgcg acggcagcag cctgctgctg cacgtgtggg acgcgcccga
     2341 cacggaggcc ttcatcgaac actacaagcg cgcgtacgaa aggcccttga tggagatctt
     2401 tgaatcatga gcccgctgct gtccggcgcc atcacgctga ccttgtggtt gtacggcctg
     2461 gccatcttgc tcatgctgtg gcgcatgctg cgcggcccct cggcgcaaga ccgcgtgctg
     2521 gccatcgacg ccatctacgt catcggcatg ctggtcgcgc tggtggtcgg catccgctac
     2581 accagcagca tgtacttcga ggcggcgctg ctgatcgcgc tgttcggctt cgtcagcacg
     2641 gcggcgatgg cgaagtttct tttgcgcggc gaggtgatcg aatgagcgaa gcacgagggg
     2701 ccccgcgcag cggggatcgg gccgcgaatt cgatcgagcg gccgaccacc aaatttgtcg
     2761 cacatcgctt ccgtccggag gccgcgcgaa tgagcgaagc acgaggggcc ccgcgcagcg
     2821 gggatcgggc cgcgaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc
     2941 gcgaatgagc gaagcacgag gggccccgcg cagcggggat cgggccgcga attcgatcga
     3001 gcggccgacc accaaatttg tcgcacatcg cttccgtccg gaggccgcgc gaatgagcga
     3061 agcacgaggg gccccgcgca gcggggatcg ggccgcgaat gcgatcgagc ggccgaccac
     3121 ccgaccggtc gaacccatcc ggcctgaagg aggccgctga ggagcaccca tgacgccgga
     3181 acccctgccc ctctggatcg aagccatcgt cgccgcgctg ctggtgtgca gcgcggtgtt
     3241 cgtgctggcc gccgcctggg ggctggtgcg gctgaaggat ttctttcagc gcatgcaccc
     3301 gccggcgctg gtgttcgtgt ggggctcgtg gtgcgtcagc ctggcgtcga tcatctactt
     3361 ctcggccctg gcccacgggc cggaattgca cgtgtggctg atcatcatcc tgctgtcgat
     3421 cacggtaccg atcaccaccg cgctgctggc gcgcgcggcg ctgttccgcg gccgccaggc
     3481 cggccagcag ctgccacccg cgctgcagcc gcgaaacgcc tcgcccgcca gcgcaccacc
     3541 gcccacgcgg tcgtccgaag aaaaataagc ctccagcgct tgtccagcaa gcgcggtcag
     3601 ctatattttc agaagcaaga cggttgatgg ccgcacgcca tcaacccggc cgcgccatct
     3661 tgcagctgtg cggcatggcc gcgctgtcgg gctcgatctg gcgcaccacg cgaaagccat
     3721 aacccgagcc ttcgacatcg aacggcacgc cgggcgtgcc ctggcgctcc atcacgccca
     3781 cggccagctg ctgctgaaac tggtgatcgg ccgcgcgcat gcgcccgcgc tggccggcca
     3841 gcgtgacgtc ggcgcgctcc agctggcgcg ccacctgcag cgggtcgctg ccgcccgcgc
     3901 gctcgatggc ctgcgccagc gcctcgatca tcagctgcat gcgcatgtgg acgtagtcct
     3961 cctgcggccg cgggaagcgc tcgcggaagc tgcggtaaaa cgccgcgctt tcaggcgtcg
     4021 gcacgttggg cagccagtcg gccaccgcca ccaccttgcc cacgcccgcc tcgccgatgg
     4081 ccgccggcgc gcccagcgcg ttgccgtaga aggtgtagaa gcggccgcca aagccggcgt
     4141 cgcgcgcggc cttgaccagc agcgtcaaat cgttgcccca gttgcccgtc agcaccgcct
     4201 gcgcgcccga ggccttgatc ttggccgcgt agggcatgaa gtccttcacg cggccgatgg
     4261 ggtgcagttc ttcgcccacg atagccacgt cgggccgctg ctcggccaac tggcgccgcg
     4321 cctcgcgcaa caccgcctgg ccaaagctgt agtcctggcc gatcaggtac atgcccttga
     4381 gctgcgcgtc gtgctggatc aactgcatca gcgccgccat gcgcatgtcg gcgtgcgcgt
     4441 cgaagcgaaa gtgccaaaag ctgcacagct cgttggtcag gatcggatcg accgccgaat
     4501 agttgaggaa caccacgcgc tgctcgggcg cgcgctggtt gtgcttattg atggcgtcga
     4561 tcagcgccgc ggccaccgac gagctgttgc cctgcagcac aaactgcgcg ccgccatcga
     4621 ccgccgcgcg cagcatcgac agcgattctt cgacccgccc cttgttgtcg aaacggtcga
     4681 gctgcagcag ccggcgcccg cgcgccgtgg ccacgccgcc gcgcgcgttg acgcgctcga
     4741 tcgcccacgc caggttgcgg tacaccgcct cgcccgtgtt ggcgaacggg cccgagagcg
     4801 actcgatcag cgccagcttc acgggcccgg cgtcggcgcc ctgggcacgc gcacccagcg
     4861 acgccgtggc cagacctgcc agcagactgc gcagcaaggc gcgacgaacg gaagaagcca
     4921 tcaattactc ctgaaaatag agctgctcgc gcatgctggg cttgcgccgg agccgaaaaa
     4981 gattgaaaaa tcaggacgct gccgccggca ccgcctcggg cgcgacggag cggtccaggt
     5041 actggccgag cagttcaaaa aagcgctgat acagcgtggc gtcgtccacc gtctcgctcg
     5101 acaccttgac catcgaatcg aggctgccct cgaccggcag cgacagcgag ccgatgccgc
     5161 ccacgcccag cgacgccgac tccttgacct tgcgcaccac gtactgctcc ttcagtccgc
     5221 tggcaaaggc cacggtggcg tcggcatcgc cgctttcacg cgcgcagacg acgcgaaatt
     5281 caagctgcac gtggtgttcg gcgcgcggct gaaagtactt gcgcgcgatc accttgtcgg
     5341 ccgtggcgtc ggtcacgatg tagccctggc tcagcaacgc gcggcgcgcc gcctcgcagg
     5401 tgcgcaccgg tggcgccacg tagtgacgca cgaaggtgtc ggcgttgaag gcttccagct
     5461 ggtagtcgcc cagcggcacg ggtgagctgg ccaggccggc acagcccgcc agcagcagcg
     5521 acgccgccag tggcagcagg cgtcgcgaca aaacgaaaaa atccatccgg tgaaaaacac
     5581 ttccaaaaac agcgcacgat ggtaacgcgc ccgcgtgacg gcgcggtcat ggggcgcacg
     5641 gccttgattt caagcgccgg ccgcgctcac ttatttgacg ccggcagcgc ttgaaatggc
     5701 gccggccatg cgcaactgag gcatcaagcg acggtggcca acccggcccg ccgcccatca
     5761 tctacaccaa aggagcaccg catgatcttt accgccacca cccccgccag ccgccgctcg
     5821 gtctacggcc ccgccctccg ctcgctcgac cgctttctgg acgacgcgct gaccgtcgcc
     5881 cgccagggcg ccacgcagtt caaggacgag ggggcgcact ggtcgctcag catcgacgtg
     5941 ccgggcgttg cgcgcgagca gttgagcatt gccatcgaag gccagaccgt gaagatcgag
     6001 accgtggaag gcgcgccgcg ccagtacaag aaggtctacg agttcgagca ggaaatcgac
     6061 gccgccgcga gccaggccaa gctggaactg ggcgtgctga cgttgacgct ggccaagctg
     6121 caacccgcga gcaaggcgac cacgctgccg atcaactgat ccgggcttga ccatgacggg
     6181 cgctgacgcg cccttgacct cggcctgcgg gcctcgatgg cggcccctcc ggcgccatga
     6241 ttttcatgcg cccgcagtgc gagtcattga aggccgcagg ccgaagtcat gcgccgaggg
     6301 cgcagtcatg ccacttcaat tttccagagc ggccaactga tccagcggcc agcgcggctt
     6361 gacgtcaaag gcgtagtcgc ggttgggcgc ttcaacgcca gcctgcagtc gcatggcggc
     6421 ggccagcgcg atcatggcgc cgttgtcggt gcacagcgcc agttcggggt agtgcacccg
     6481 ggcgccgcgt gcggcgcagg cggcgttcag ctgctcgcgc agcgccgcgt tggcgcccac
     6541 gccgccggcc accaccaggc gattcagccc ggtctgcgcc agcgccttga gcgacttttt
     6601 caccagcacc tcgacgatgg ccgcctgcgt gctggccgcc aggtcggcgc gctgctgctc
     6661 gcagggcgag ccttcgaact tgcgcgcctg cgtcaacacc gccgtcttca ggccggcaaa
     6721 cgaaaagtcc aggtcgccgc tgtgcagcag cgggcgcggc agcttgaacg acgtggcatc
     6781 gccaaaatcg gccagccgcg ccagcgccgg gccgccgggg tagcccagcc cgagcagctt
     6841 ggcggacttg tcgaacgcct cgccggccgc gtcgtcaatc gtctcgccca gcagctcgta
     6901 ttgccccacg ccatcgacgc gcatcagctg cgtgtggccg ccgctgacca gcaaggcgac
     6961 aaacggaaat tcgggcgggt cggcgcttaa aaacggcgac agcaaatgcc cttcgaggtg
     7021 gtgcacgccc agcaccggtt tgcgcaacgc cgcgcccagc gcgcacgcca cgccggcgcc
     7081 caccagcagc gcgccggcca gaccggggcc gcgtgtgtac gctacgacat caatagctgc
     7141 cagcgcttgt cccgcctgcg ccagcgcctg atttgccaat ggaatgatgc ggcggatgtg
     7201 gtcgcgactg gccagctcgg gcaccacgcc gccgtagtcg gcgtgcatgg cgatctggct
     7261 gtgcagggcc tggcccagca gctcgggcac cgcgccctcg cgcgtgcgca ccagcgccac
     7321 gccggtttcg tcgcacgacg attcgatgcc aagaacaagc agggccatgc gcggaattat
     7381 ccgcgccgcc cggcgccctt gcagccgtca ggtcagatcg cccgagatgt agctgcccag
     7441 ctgcgagatc aaaaattcct gctcggccat ggtggcgttg accaggtcgc caatcgacac
     7501 cagcccctgc accgcgccgt cgctcaccac cggcaggtgg cggatcttgg cctgcgtcat
     7561 gatgcccatg cactcttgca ccgtctggcg cgggctgacg acgtagagct tggcggtcat
     7621 cacgtcggcc agcggcgtgt cggccgatgc gagccccttg agcgccagct tgcgggcgta
     7681 gtctcgctcg gtgaagatgc ccagcaacgc ctcgccctgc atgatgagaa ccgagccgat
     7741 gttgcggtcg gccatcagtt gcagcgcgtc gcgcaccgtg gcggtgggcg acagcgacgc
     7801 caggctgcgg gcatcacgct tggacaggat gtcggcgacg gtcggcatgg ttctcctcca
     7861 aaaaatagtg ggcgtggcac agaatggctg ggcgtgtcat tttgcacgct tcgtgccagc
     7921 cgggatgtga cgaggcgcaa gtgtgcggcc ccaacgcggc gcggcatggg tggcacgacc
     7981 cggggcggcg gcacaatggg gcttggccta gcttgggaaa ctatcttttc gatagctgct
     8041 cgcgcttgcc catcaagcgc tgcagcccta ttttccttaa aagcaactcc gagacccacc
     8101 cccatgaacc cgacgcccct tgctgtcatc tcctccatgg ccaccaaagc gctgttgacc
     8161 gagctggtgg ccgagttcca gcgcctgcac ccgggccgcg cggtggcgct tgaatcggtg
     8221 ggcggcgtcg atgccgcccg gcgcgtggcc gccggggagg cgctggacgc ggtggtgctg
     8281 ggctcggacg ccatcgacca gctgatcgcc agcggccatg tgcgggccgg cagccgggtg
     8341 gacatcgtgc gttcgggcgt cgccatcgcc gtgcgcgccg gcgcaccgcg gccggacacg
     8401 ggcagcgagg cggcgctgaa ggccgccgtg ctggcggcgc gcagcgtggg ttactcgacg
     8461 gggccgagcg gcgtggcgct ggcgcgcctg tttgagcgct ggggcatcgc cgatcaagtg
     8521 cgggccaagc tggtcacgcc gccgccgggc acgccggtcg gcgcgctgat cgcgtcgggc
     8581 gaggtcgaac tcggctttca gcaactgagc gagctgatgc atttgccggg catcgacgtg
     8641 atcggcccgc tgccgcccga gatgcagatc gagaccgtgt ttgccggcgg cgtggcggcc
     8701 acctcgacac agaccgacgc ggcgcgcgag ctgctcgcct ttctggcctc ggcgccgctg
     8761 gccgctgcca agcaaaaaca cggtatggcg ccggcctgac ggcgcaaaaa aaacggcgcc
     8821 acgcgggcgc cgcttttcat gaaggcgtca gcgcttcaat agcgattgcc gccgccgcga
     8881 tagccaccgc caccgccgcc gcctgagcga ccgccaccgc cgtaaccacc gccgccgccg
     8941 ccaccatagc cgccgccacc acccgagcgc tcttcgcgcg ggcgggcctc gttcaccacc
     9001 agcgcgcggc cgccttgcga ctggccgttg agcgcctcga tggcggccag cgcctcggca
     9061 tcgttggcca tttcgacaaa gccgaagccg cgtgagcggc ccgagtcgcg ctccatcgcc
     9121 accgaggcgg agctcacgtt gccgtgctcg gcaaacatct gttgcaggtc ttcgtcacga
     9181 accgaatagg caagattgcc gacgtaaagt ttcttggcca tcgctggctc ctttcaagtt
     9241 ggatcgaccg tcgatccatt caaaccggtt aaaaaattca atgccgccgc acgcccgggc
     9301 gcggcccgcg cggcgcctgg cccgccaggt ggcggaaggt gatgcgaccc ttgcccaggt
     9361 cgtagggcga gagctcgagc gacacgaagt cgcccgccag gatgcggatg cggtgcttgc
     9421 gcatgcggcc ggccgaatag gccaccaact ggtggccgtt gtcgagcgtc acgcggtagc
     9481 gcgcgtcggg cagcacttcg tccacgcggc cgcgcatttc gatcaggtct tctttggaca
     9541 tgggactttc aagcgccgca ggcggcgtgc acccaggcac ggccctgggc ggccgcgtgg
     9601 cgcaaggcgc catcggcggt ggtgaagcgc ccgatcaggc gcagcacgcg atcagaactg
     9661 gcctggccac ggccgctgcg gatggagacc gaagcgctgt aggcgccatc atcgagaagg
     9721 cgggtgagcg gagagacgac gtagcgtccg agagaaaaac aagggttgga catcaagaga
     9781 gcaaaacgcc gggccatcgc aggcatccgg ccagacaaaa cgggttaaga caacgatcaa
     9841 gcggccagcg cgcaaaaaag cggcaccgca tgaagatcga ctggggaaat cagccaagtc
     9901 gttgaatcgt tatcgacccg gccggaaagc tcgccgcaga gatgggcatg cgcaggatcg
     9961 ggggcagccg cgtgggggcg tgatcgatct gcgagacatg gggcaagcgc tgggcgctgg
    10021 cccgcgccgt ccaaggagag caacggacgg cgatacgcca gtgtaaccgc aacacgctgg
    10081 cggcgctgta ggccagcgcc gcaggctcca tggcgccctt gaatgggaca atcaccacct
    10141 ctgccgagcc ccctgccccg tgcgatgagc ctcgccccga ccgaccccga tgtgcgcgcc
    10201 ctgctggcgg gcacgctggc gcaaacccga tccagcgccg ccgtcatctg cctgtgcgcc
    10261 gcctggtgcg gcacctgccg cgaatatgcg ccgctgtttg agcaggtcgc ggcggcgcac
    10321 ccgcacctgg catttcgctg ggtcgacatc gaggacgagg ccgacgcgct gggcgatctg
    10381 gacatcgaca cctttcccac gctggtcgtc ggcgcgggcg cggtgcagtt tgccggcccg
    10441 gtgctgcccc aggccgggca aatcgagcgc ctgctgaaaa gtctgggcct ttgacaacgg
    10501 ccgtgagccg gcacggcaac tgctttattc tttcttgact cgaatcaatt gagcggccac
    10561 ccacgcccac ccaaaataaa cctcatggga atcagccctt atctcaaaga aatcggccgc
    10621 ggcagcgccg gcgcgcgcca cctcacgcgc gagcaggccg ccgacctgat gggccagttg
    10681 ctcgacggcc aatgcaccga tctggaactg ggcgcctttt gcatcgccat gcgcgtgaag
    10741 ggcgaaacgc cggcggaaat ggccggtttt ctggacgccg tcgacacccg catcaccaag
    10801 ctgccggcca gcacccgccc cgtggtggtg ctgcccagct acaacggcgc gcgcaagctg
    10861 ccggtgctga cgccgctctt ggcgctgctg ctggcacgca aggggctgcc ggtgctgatc
    10921 cacggcgcgg ccaccgaaga ccgccgcgtt tcaacgcaag aagtgctctc cgcgcttggc
    10981 ctgtctgcgc aagcagctat ccaatccata gcaaccgggc aggtgcattt tgtcgccacc
    11041 gacagcctgc acccgggcct gaagcgcctt ttggacgtgc gccgcaccat cggcctgcgc
    11101 aattcggcgc acagcctggt caagctgatg aacccggtgg ccggcacggc actggtggtg
    11161 ggcagctaca cgcaccccga atacgccgaa tccatgaccg ccaccttccg actgatgggc
    11221 accgacgcca tgctgctgcg cggcaccgag ggcgagcccg tggccgaccc gcgccgcacg
    11281 ccacgcatgg acgtctttct gcgcggcgaa atcgacaccg tgcaggccat gcgcgaaggc
    11341 ccgctggcca gcctgccctc gctgccgaca cagatcgacg ccgccagcac cgcgcactac
    11401 atccgcgccg tgctggaggg gctgatcccg ctgccggaac ccatcggcat ccaggccgac
    11461 cacatcgtgc gcgcttgcaa ggccctcgaa gcggtgggcg atagctcgtg cctgcaggcc
    11521 ttggcggcgt gaccgacatg aacaccccac tcggccaagt caccctcgtc ggcgccggcc
    11581 ccggcgatcc tgagttgctc acgctcaagg ccgcccgcgc catacaagcc gccagcgtca
    11641 tcctggtcga cgacctggtg ggcgaaggcg tgctggccca cgcgcggcct gaggcgcgca
    11701 tcacctacgt cggcaagcgc ggcggctgca aaagcacgcc gcaggcgacc attgagcagc
    11761 tgatgctcga cgccgcgcgc gcgggcgaga acgtggtgcg gctgaaaggc ggcgacccgt
    11821 tcatcttcgg ccgcggcggc gaggaggtcg agagcctgcg ccgcgccggc atcgagccgg
    11881 tggtcatcaa cggcatcacg tcgggcctgg ccgccatcac cagcctgggc gcgccgctca
    11941 cgcaccgcga gcacgcgcag ggcgtggtct tcgtcaccgg ccacccgcaa aaggacgggc
    12001 cgggcctcga ctgggtgcgc ctgggcgaat cggcgcgcga cctgcgcctc acgctggtga
    12061 tctacatggg catcagcagt gcggcgcgca tccagtcggg tttgttggcc gctctgccgg
    12121 gcgacacacc agtcgctgtc atcgagcgcg ccacgttgaa aaatcagcgc cagtgcttga
    12181 ccagcctgcg tgagctgctc tcgggtttgc agcaatccgg tttgggcagc ccggccatca
    12241 tggtggtcgg cgatgtggtg acgggcgtgc gcgaggcgct gcgtgcgccg gcgctggccg
    12301 atcagtcggc ctgacgcggc gcggcgcccg ccagcagagc gcgcaccggg tcgctgcgcg
    12361 cgcaatcgtg caccgtgcgg ccgcgcgcat ccggcagatc ggcgcgcacg cccgctgcca
    12421 gaaaggcagt cacggtcttt gcttgcgcct gccgcgccag cggcgcaccc gaaccaccgc
    12481 gccccgtgtc ctgaaccgcc agatgaaacg gcgtcgagcc ggcccgggtt gcgcacgcgc
    12541 ggctcagcgc ccaccgccag cagctcgatg accgccgccg ctcagcgcgt gcgcacggcc
    12601 cggtgcagcg cggtggcgcc gttgcggtcc tgcgcgtgaa ggtcggcacc ggcggccagc
    12661 aggcagcgca gcgtggccac ttgtgccacg gcatcgcacg ccgggtgcgt gatgaagccg
    12721 tccgccgcgt agtgcaacgg cgtgctgcgc cggtggttgg cctgcgcgtt cacgtcggcg
    12781 ccggcggcca accactgttc aacgatcgag gttcgatagc cggccgccgc cagatgcagc
    12841 gccgtgtcgc ccgcgtacag ccagtgaaag atgccctcct cgtacagccg tgcctgcgct
    12901 accgacgcct gcgccaggcg cgcgtcggcg gccagcagag cggcaacgcg ctgcgtatcg
    12961 tcatcgacga tggcttccag cagattctca aaccccacgc cgcgcgcctt cttgttcagt
    13021 ggccgtgctc cagcaactgc tgcaacacca ccgcctcgtt gtgcacctga tcgcgcgcgg
    13081 cgtagatcag cgtgatgttg ccctgatgcg ccagcttttt cagctcgtca aacgccggat
    13141 tgcccttgag ttcggcgcgg tagcgggttt gaaactcagt ccacttggcc gggtcatggc
    13201 caaaccactc gcgcagttcg gtggacggcg ccacgtcttt cagccacaca tcgaacttgg
    13261 ccttgacctt ggccaacccg cgcggccaga ggcggtcgac caggatgcgc ttgccgtccg
    13321 atgccgtggg cgggtcgtac gcgcgcttga gaaaaatcgc catggctcgc tcctgttgtc
    13381 gccgcccggc cagtttaggc cgccgcgtgg cgcgcggaca gcgcggcagc gctggccggc
    13441 gcggcgcgcc aagcgccgta ttgcaaccga atgttcaccg aatggtccag caggcgtgat
    13501 aaaagtcacg cttttcaagc ttgaggaacc ccgttttgat gcagtcaaac cgtaccgcca
    13561 ccaatgccct gatcgccctg tcgctcggcc tgatggcccc agccgccttt gccgacgact
    13621 acccgccgcg caaggcgggc ctgtggcaga tgaccaccaa gagcagcacc ggcggcgcgc
    13681 ccgaagtggt tcaccaatgc atcgacgcgg ccagcgacaa ggccatgcag caggccggca
    13741 aggcgatggc gcagcagatg tgccaggacg tcaagctgcg ccgcgacggc gacaaggtca
    13801 tcattgaatc cacttgcgcc atgtcgccca agtcgaagat ggtcagccgc ggcgtgatga
    13861 gcggcgacta ccagagcgct taccgcgaag aatccgaaac ccgcttcgat ccgccgatgg
    13921 ccgggatcca gaacgtgcgc accacgacag aggcgcgctg ggttggcgcc tgcaaggccg
    13981 gccagcgacc gggcgacgtg gtcacggccg acggcatgaa gatgaacctg ctcgacatgg
    14041 tgaaggccgg gccggccatg cgcggcaaag cgcccgcccg gtaaatcaaa gccaaatcag
    14101 gccctggcgc ctgtcagaca agcgcgagca gctatgtttt ttatagtttt ctgaaattca
    14161 cgccttctcg tccggcactt ccacatcgtc cagaaacgac gccgggcacg aagaacaagc
    14221 cgccggtcac cgccgtgctg aagtcgagga tgcggtcgta attgcccggc gggttgccga
    14281 tgaacatgtt gttcagcatc ttttcgatgc ggtccggcga gcgcgcgtag ccgatgaaat
    14341 aggtgccgaa ctcgcccttg ccgacttggc caaacggcat gttgtcgcgc aggatttgca
    14401 gttgctggcc gccttcttcg atgttggtta gcaccttgtg cgcgtagctg gctttatcga
    14461 acgggcgctg ctcgatgtcg gacggcttct tgcggccgat gatggtctct tgccgctcca
    14521 ccggcagcgc gttccaggcc ttcaggttgt gcaggtactt ctggatgatc acgtagctgc
    14581 cgcccgtgaa accggggtct tcgtcgccga tcagcgtggc ctgcacggcg gcctgggcgg
    14641 acgggttctc ggtgccgtcg acaaagccga gcagatcccg ctcgtcgaag aacttgaagc
    14701 cgtgcacctc gtccaccacg gtggccgcgt cttccaggcg cgacatgatc gcggccgcca
    14761 gttcaaagca catgtc
//
LOCUS       DAOMVO010000025        14425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_914_length_14425_cov_11.894085, whole genome shotgun sequence.
ACCESSION   DAOMVO010000025 DAOMVO010000000
VERSION     DAOMVO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14425
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_914_length_14425_cov_11.894085"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKV30_02595"
     CDS             complement(<1..381)
                     /locus_tag="PKV30_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRL29141.1"
                     /translation="MSRFPRSLVQASALACIAVAALVACGGSDDKYPTLTGDKPLVIG
                     HRGASGYLPDHTLEGYKKAIELGADFIEPDLVATKDGVLVARHEPNISATTNVKDHPE
                     FASRKRKAMVDGFEEEGWFVSDFTL"
     gene            556..1758
                     /locus_tag="PKV30_02600"
     CDS             556..1758
                     /locus_tag="PKV30_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRL29142.1"
                     /translation="MSPLLLVVLLALLLGIQPVTTDVYLPALPALQADLRAPMAEVQL
                     TFAALLLAFGTSQLVWGPLSDRFGRRPILLAGMALYVLASIACVLAPSMPALVWARVA
                     QGAAIGAAVMCARAIVRDLYPPLEGARMMSRGLSGLGVIAVVCAPLGGLLTDWLHWRA
                     AMSALVVFGAGTLVLLALRFRETIPARNPDALAPGMLWRRWRQIAANPTFITYSLLST
                     GSYVALFIFLASSSFVLIESLGLSRVAYGAVMAGNSALYIVGTIACRRLLVRWGIRRT
                     VFAAGFVTFAAGALMAALAWFGWGQPWYGVWAVVLPQYLFILAHGVHQPVGQSGAVAP
                     FPQMAGAASALNGFITMGAAFGMGLWLGRAMDGTPRPAALGFLLWCTVIALTAWTLVQ
                     KHGDTGQH"
     gene            1736..2713
                     /gene="miaA"
                     /locus_tag="PKV30_02605"
     CDS             1736..2713
                     /gene="miaA"
                     /locus_tag="PKV30_02605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRL29143.1"
                     /translation="MATPASTEGGGPPPLYLAGPTASGKSAVALALAERVPAEIISVD
                     SALVYRGMDIGTAKPTPAERAAAPHHLIDIRDPATPYSAAEFVRDAQRLIADIRARGR
                     LPVLVGGTMLYFKALFEGLDDLPPADAAVRRQIEERGAQEGWPALHAELARVDPITAA
                     RLAPGDSQRIQRALEVWQLTGQPMSALHTTKSGAARAQQTGAGSLFSLEPADRAWLHG
                     RIAQRFGDMLAGGLVDEVTALRARGDLTPDMPSMRCVGYRQVWDTLDGRAPADTLRER
                     GIAATRQLAKRQLTWLRGMPERQVLAVDDKTSAVEMAQRLLDRTRLDNF"
     gene            2879..4729
                     /locus_tag="PKV30_02610"
     CDS             2879..4729
                     /locus_tag="PKV30_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRL29144.1"
                     /translation="MSQQISDPAEDTAVVTTAARPRAPEAPGPHQAATLPPAPLPGDS
                     GLGRIAGLSALMSAVLAACGGGSGDAPSVDPTPMPSPTPAPPVVPTPAPGTPSPAPAP
                     ATPTHIAAAEAARFLQQAQFSASDADIAAVQAQGYAGWLDAQMARGTSTTGWDWLMGQ
                     GYNQFSIRYNNSYADHMIWNQLLTAPDPVRKRVALAWSEIMVVSTSTLDGTWPSFAMA
                     AYWDLLNQHAFGNYRQLLEAITLNPAMGAFLNTRGNKKANASGRVPDENYAREVMQLF
                     SIGLYELNTDGTLRGGAAKETYGPADVSNLARIFTGYNLNSPNKLHETDPQPYRVPMV
                     LTAADHSPEPATFLGQTVASGDGATRLRQALDIIFNHPNVGPFIARQLIQRLVTSDPS
                     AAYVGRVAAVFNNNGAGVRGDLRAVTKAVLLDADARQNPSLQSPSWGKLREPMIRFVQ
                     WGRTFGATSADNRWILWDMSDASTQLGQSPLRSPSVFNFFRPGYVPPGTELARRGQTA
                     PEFQLTNENSVAGYINFMHGVIAWGYGAAASRISVPDYTREMALVNDPAALVDRLNLL
                     LAGGQLSAATLTTIRNAITTINPGSDWGRKTRVWSAIVLVMACPEYLVQK"
     gene            4741..6141
                     /locus_tag="PKV30_02615"
     CDS             4741..6141
                     /locus_tag="PKV30_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRL29145.1"
                     /translation="MTPTQRIARRTFLRRIGHLGIAGTAAPWAINLAAVGEAAAFNAD
                     GYKALVCIFLYGGNDNGNTVVPYDTSGHAEYLTVRGALATARANLAATALTGAGLPAG
                     RQYALAPQLTGLKALFDKKQLAVQLNVGPLLRPTTLAQFQARSVPLPPKLFSHNDQQS
                     VWQSSHAEGSVKGWGGNLGDLALAGNGHSLFTCMSVTGNAVFLSGDSALAYQIGAGGA
                     VSINGVTSTDGMYGSAACRTALKDLITQTRPQVLENELNLVTSRSITSQAQLAGALDS
                     SATQFDALLPDKVGDRTMALNRQLKMVARLIAGRQQLGLKRQVFLVSLGGFDTHDNLN
                     TNHPALLAHVGNAMAGFQQALDSLGVGQQVTTFTASDFGRTMTSNGDGSDHGWGSHHF
                     VMGGAVKGGAFYGSAPSALLKGPEDVGQGRLLPTTSVDQYAATMARWFGVADSEMTQI
                     APNIGYFDSVNLGFMT"
     gene            6264..>7244
                     /locus_tag="PKV30_02620"
     CDS             6264..>7244
                     /locus_tag="PKV30_02620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRL29146.1"
                     /translation="MSSTLHPMLNVAIRAARAAGAIINRAALDIESVRISQKQVNDFV
                     TEIDHASEDAIIDTLLTAYPHHGMHAEESGTTRGNPKADHVWIIDPLDGTTNFIHGFP
                     VYCVSIALAVKGRIEQAVVYDPTRNDLFTATRGRGAYLNDRRIRVSKRTQLNQSLVAT
                     GFPFRKGDDFPTYLKMMGDVMQRTAGLRRPGAAALDLAYVAAGFTDGFFETGLKVWDV
                     AAGSLLVTEAGGLMGNFTGETGDLEQGECLAGNPRVYAQLVQVLRQYSRYDSAERTSD
                     GRKEQISLKKPATSTKNDDAAFDAWAKDAATEAAADSAAPADAASGADHSD"
     assembly_gap    7245..7254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7262..8017
                     /gene="trmB"
                     /locus_tag="PKV30_02625"
     CDS             7262..8017
                     /gene="trmB"
                     /locus_tag="PKV30_02625"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRL29147.1"
                     /translation="MSTPPDAAPAGVAHPRPIRSFVTRAGRTTAAQARALAELGPRHV
                     LPYDPSTTDLVAACADPASADGQNRSKTVLEIGFGMGHATAHIAALMPDTLFIGCEVH
                     EPGVGALLKSIGEQNLDNLRIVQHDAVEVLRHMIAPASLHGVHIFFPDPWHKKRHHKR
                     RLIQPPFVRLLAERIAPGGYLHCATDWQPYAEQMLAVLGAEPLLTNTAADYAPRPDYR
                     PETKFERRGLRLGHGVWDLVFRRNDRPATPNAT"
     gene            8073..9074
                     /locus_tag="PKV30_02630"
     CDS             8073..9074
                     /locus_tag="PKV30_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRL29148.1"
                     /translation="MSRPPRPSHLPLRDGVSASCVVVRPGDAPTALDFLAARLAVLSR
                     DEWAERLARGEVLDAQGQPLNASTPCHPGQRIYYYRRLAAEPEPLVSEHIAFQDEWLV
                     VADKPHFMPVTPSGRFVQQSLLVRLKRRLGLADLSPVHRIDRETAGLVVFAVQTHTRD
                     AYQALFRDRRVHKLYEAVAPHRSDLALPCTHRSRLAPDAERFFLSREVAGEPNSETHI
                     ERAQPLGTHALYHLRPVTGQRHQLRLHMMALGVPILGDGFYPEVLRGPGEPDDAENPL
                     QLLARALAFDDPVTGEQRRFSSSLTLRAAQAASPAPAAGADQFDDKVPRLVMGASGA"
     gene            complement(9023..9484)
                     /locus_tag="PKV30_02635"
     CDS             complement(9023..9484)
                     /locus_tag="PKV30_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29149.1"
                     /translation="MQSATLYVMRHVGLVGRAECWATRTADGRDGVLLVIQTPQVVPA
                     AARDEMQQYFRRKLMELGELRGEPFRLLIRDGEEQSLAHRARTDSSSARIATLIAAAN
                     QRPEADAPAEQLLADLRTTVRQRTPERRQARADSDYAPLAPMTNLGTLSSN"
     gene            9657..10583
                     /locus_tag="PKV30_02640"
     CDS             9657..10583
                     /locus_tag="PKV30_02640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL29150.1"
                     /translation="MSALPENLILHERGWLSSNNLLCLGDAPALIDTGHVKHADETVR
                     QVRQALGDQPLADIAHTHLHSDHCGGTGALQAAWPAARTWVPAPSLAHVQAWDEDALT
                     FGDTAQRCARFVAQHALVPGQTVRLGDFDWQIHAAPGHDALAVLLFEPTHGVLVAGDA
                     MWEHGVGVIFPHIDGTDGFDTFTDTLALIERLDPAVVVPGHGAIFERQGGAITAAIAR
                     ARARIDQFNANPAAHALHAAKVLIKYQMMDVERMPRADFERWLDSARALHALHQQHRP
                     DMVWRDWLAHILAPMFGKGVLRQDAYTVFDGA"
     gene            complement(10611..11598)
                     /locus_tag="PKV30_02645"
                     /pseudo
     CDS             complement(10611..11598)
                     /locus_tag="PKV30_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
     gene            complement(11696..12814)
                     /locus_tag="PKV30_02650"
     CDS             complement(11696..12814)
                     /locus_tag="PKV30_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRL29151.1"
                     /translation="MNTTRRALIGRAAGLGAAAALPGALLAQTSTPTKAARRFDPEPG
                     DWRTFEVTTRIDLPATTSATRVWVPLPSVQTPWQQTLGHTFGSNGQARLASDGAQGVR
                     MVAAEFAPGAAQPFVEVTSRVRTQSRDESQARRRAAEDSATLREALKPTRLLPTDGVV
                     RDTARQATRGARGDEAKARAIYDWVVANAYREPKVRGCGEGDIKTMLETGNLGGKCAD
                     LNALFVGLCRSVGLPARDVYGIRLAPSAFGYRELGGNPTSLKGAQHCRAEVYLQARGW
                     TAMDPADVAKVMRHETREWIKQAGNPIVKPVYEGLFGGWEGNWMAYNTAHDVKLPGAQ
                     EGELGFFMYPVAENADGRFDSYAPDDFKYQITARQVAA"
     gene            complement(12934..13407)
                     /locus_tag="PKV30_02655"
     CDS             complement(12934..13407)
                     /locus_tag="PKV30_02655"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL29152.1"
                     /translation="MLPSISFSQHDIKTWRRSSSMPPLKGTDLNGREWNLAELKGQSV
                     LLNFWATWCAPCKEEMPTLQTLHELGEADLVLLTVNVRESIPHVQRYARSTGLTLPIL
                     PDPKGVIAKAWGIVVYPSTLLIDTTGTPRQFVTGAVDWSGSEALGWLRALRQPAR"
     gene            complement(13497..>14425)
                     /locus_tag="PKV30_02660"
     CDS             complement(13497..>14425)
                     /locus_tag="PKV30_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRL29153.1"
                     /translation="THLMHKALREVLGAHVQQKGSLVDAEKTRFDFAHNAPVTPEQIT
                     EIERRVNEEILANAATDASVMDLDSAQKTGAMMLFGEKYGETVRVLTIGSSKELCGGT
                     HVRRTGDIGLFKIVSEGGVAAGVRRIEAITGANALEYTQGLEASLHSVAATLRTPPAE
                     VQARLGQVLDQVKALEKEIAQLKGKLASSQGDELVNQAVDVNGLKVLAATLQGADAKT
                     LRETMDKLKDKLKTAAIVLAAVEGDKVQLAAGVTKDAIGKVKAGELVNFVAQQVGGKG
                     GGKPDMAMAGGTDASGLPKALQSVQGWVAERV"
BASE COUNT         2179 a         5181 c         4785 g         2270 t
ORIGIN      
        1 cagcgtgaag tcgctgacga accagccttc ttcctcaaag ccatcgacca tggccttgcg
       61 cttgcggctg gcaaattcgg ggtggtcctt gacgttggtg gtggcgctga tgttgggttc
      121 gtggcgcgcc accagcacgc cgtctttcgt ggccaccagg tcgggctcga tgaagtcggc
      181 gcccagctcg atggccttct tgtagccctc cagcgtgtgg tcgggcaggt agccggaagc
      241 gccccggtgg ccgatgacga gcggcttgtc gccggtcagc gtggggtact tgtcgtcgct
      301 gccgccgcag gcgaccaggg cggccacggc gatgcaagcg agcgcgctgg cttgcaccag
      361 ggaacgggga aaacggctca tcgactctct cctctcaaaa aaatacgggg tgaagccgcc
      421 actgtatgac ttggtgatga ctgttcgacg acagcgcgcc ggggcagccg ctgggcgaag
      481 gcgttctgct accctcgccg cgtgccgccg cccgactctt cgccccgccc gtcttccgag
      541 gcgccaccgt ccgccatgtc gccgctgctg ttggtcgtgc tgctggcgct gctgctgggc
      601 attcagcccg tcaccaccga cgtctacctg cccgcgctgc cggcgctgca ggccgatttg
      661 cgcgcgccga tggccgaggt gcagctcacc tttgccgccc tgctgctggc gtttggcacc
      721 tcgcagctgg tgtgggggcc gctgtcggac cggttcgggc gccggccgat cttgctggcc
      781 ggcatggcgc tgtacgtgct ggcgtccatc gcctgcgtgc tggcgcccag catgccggcg
      841 ctggtgtggg cgcgcgtggc gcagggcgcg gccattggcg cggcggtgat gtgcgcgcgc
      901 gccatcgtgc gcgacctgta cccgccgctc gaaggcgcgc gcatgatgtc gcgcggcctc
      961 tcgggcctgg gcgtgatcgc cgtggtctgc gcgccgctgg gcggcctgct gaccgactgg
     1021 ctgcactggc gtgccgccat gtccgcgctg gtggtgtttg gcgccggcac gctggtgctg
     1081 ctggcgctgc gctttcgcga gacgattccg gcgcgcaacc ccgacgcgct ggcgcccggc
     1141 atgttgtggc gccgctggcg ccagattgcc gccaacccga ccttcatcac ctattcgctg
     1201 ctgtcgaccg gctcgtacgt ggctctgttc atctttctgg ccagctcgtc cttcgtgctg
     1261 atcgagtcgc tgggcttgag ccgcgtggcc tatggcgcgg tgatggcggg caattcggcg
     1321 ctctacatcg tgggcaccat cgcctgccgc cgcctgctgg tgcgctgggg catccggcgc
     1381 accgtgttcg cggccggctt tgtgaccttt gccgccgggg cgctgatggc ggcgctggcc
     1441 tggttcggct ggggccagcc gtggtacggc gtgtgggcgg tggtgctgcc gcagtacctg
     1501 ttcatcctgg cgcacggcgt gcaccagccg gtcggccaga gcggcgcggt ggcgccgttt
     1561 ccgcagatgg cgggcgcggc gtcggcgctc aatggcttca tcaccatggg cgccgcgttt
     1621 ggtatgggcc tgtggctcgg ccgcgccatg gacggcacgc cgcgcccggc ggcgctgggt
     1681 tttctgctgt ggtgcacggt gatcgccctc accgcctgga cgctggtgca aaagcatggc
     1741 gacaccggcc agcactgaag gtggcggccc gccgcccctg tacctggccg gccccaccgc
     1801 cagcggcaag agcgccgtcg cgctggcgct ggccgagcgg gtgccggccg agatcatcag
     1861 cgttgattcg gcgctggtgt accgcggcat ggacatcggc accgccaagc cgacgccggc
     1921 cgagcgtgcg gcggcgcccc accacctgat cgacatccgc gacccggcca cgccctacag
     1981 cgcggccgag ttcgtgcgcg atgcgcagcg cttgattgcc gacatccgcg cccgcggccg
     2041 cctgccggtg ctggtcggag gcaccatgct gtatttcaag gcgctgttcg aaggcttgga
     2101 cgatttgccg cccgccgacg ccgccgtgcg ccgccagatt gaggagcgcg gggcgcagga
     2161 aggctggccg gcgctgcacg ccgaactggc gcgcgtcgac cccatcaccg ccgcacgcct
     2221 ggcccccggc gacagccagc gcattcagcg cgcgctggag gtgtggcaac tgaccggcca
     2281 gcccatgtcc gccttgcaca ctacaaaatc aggagctgct cgcgcacaac agacaggcgc
     2341 tggaagcctt ttttccttgg aaccggctga tcgcgcctgg ctgcatggcc gcatcgccca
     2401 gcgctttggt gacatgctgg ccggcggcct ggtcgatgaa gtgaccgccc tgcgcgcgcg
     2461 tggcgacctg acgcccgaca tgccatcgat gcgctgcgtc ggctaccggc aggtgtggga
     2521 cacgctggac ggccgcgccc cggccgacac cctgcgcgaa cgcggcatcg ccgccacgcg
     2581 gcaacttgcc aagcggcaat tgacctggtt gcgagggatg ccggagcggc aggtgctggc
     2641 ggtagacgac aaaacgtcgg ccgtcgaaat ggcacagcga ttgcttgatc ggacgcgtct
     2701 cgacaatttt tgacgctttc gcgtttttct ttcgcccgtt gcgcgcatcg cgcgtggttt
     2761 acacgttttt acgccggcac atcctggcag gcgccaatcc ccgctttaat tccgcccgca
     2821 gtgtcgatgc gcaactgggc cgcgctgcag caaaaaaatt caaccccgga ggatggagat
     2881 gagtcaacag atcagcgacc cggctgaaga cacggccgtg gtcacgacag ctgcgcgccc
     2941 gcgcgcgccc gaggccccgg gccctcacca ggcggccacc ttgccgccgg cgccgttgcc
     3001 aggcgattca ggccttggcc gcatcgccgg actctcggcc ctgatgtcgg cggtgctcgc
     3061 cgcctgcggt ggaggcagcg gcgatgcgcc gagcgtcgat ccaacgccga tgccgagccc
     3121 aacgcccgcg cccccggtcg tgccgacccc cgcacccggc acgcccagcc cagcgccagc
     3181 gccggccaca cccacccaca tcgccgccgc cgaagccgcg cgcttcctgc agcaggcgca
     3241 gttctccgca tccgacgccg acatcgccgc cgttcaggcc caaggctacg ccggctggct
     3301 cgacgcgcaa atggcgcgcg gcaccagcac caccggctgg gactggctga tgggccaggg
     3361 ctacaaccag ttcagcatcc gctacaacaa cagctacgcc gatcacatga tctggaacca
     3421 gctgctgacc gcgcccgacc cggtgcgcaa gcgcgtggcg ctggcgtggt cagagatcat
     3481 ggtggtgtcc acctccacgc tcgacggcac ctggccctcg tttgccatgg cggcgtactg
     3541 ggacctgctc aaccagcatg ccttcggcaa ctaccggcaa ttgctggagg cgatcacgct
     3601 caacccggcc atgggcgcct tcctcaacac gcgcggcaac aagaaggcca acgccagcgg
     3661 ccgcgtgccg gacgagaact atgcgcgcga agtgatgcag ctgttctcca tcggcctgta
     3721 cgagctgaac accgacggca cgctgcgcgg cggcgcggcg aaggaaacct acggccccgc
     3781 cgatgtcagc aacctggccc gcatcttcac cggctacaac ctgaattcgc ccaacaagct
     3841 gcacgaaacc gacccgcagc cctatcgcgt gccgatggtg ctgaccgccg ccgaccattc
     3901 gcccgagccg gccacgtttc tgggccagac cgtggcctcg ggcgacggcg ccacgcggct
     3961 gcgccaggcg ctcgacatca tcttcaacca ccccaacgtc gggccgttca tcgcacggca
     4021 gctgatccag cgcctggtca ccagcgaccc gagcgcggcc tacgtgggcc gcgtggccgc
     4081 cgtcttcaac aacaatggcg ccggcgtgcg cggcgacctg cgcgccgtga ccaaggccgt
     4141 gctgctggac gccgacgcgc ggcaaaaccc gtcgctgcaa agcccctcct ggggcaagct
     4201 gcgcgagccc atgatccgct tcgtgcaatg gggccgcacc tttggcgcca cctcggccga
     4261 caaccgctgg atcctgtggg acatgtcgga cgcctccacg caactcggcc agagcccgct
     4321 gcgcagcccc tcggtgttca atttcttcag gcccggctac gtgccgccgg gcaccgagct
     4381 ggcccggcgc ggccagacgg cgcccgagtt ccagctgacg aacgaaaact cggtcgccgg
     4441 ctacatcaac ttcatgcacg gcgtcatcgc ctggggctat ggcgccgcgg ccagccgcat
     4501 cagcgtgccc gactacacgc gcgaaatggc gctggtgaac gaccccgccg cgctggtcga
     4561 tcggctcaac ctgctgctgg ccggcgggca actgtcggcc gccacgctga ccaccatccg
     4621 caacgccatc accaccatca acccgggcag cgactggggc cgcaagacgc gcgtctggtc
     4681 cgccattgtg ctggtgatgg cttgccccga ataccttgtg cagaagtgag agcagcgacc
     4741 atgacgccaa cccaacgcat cgcgcgccgc acctttttgc gccgcatcgg ccacctcggc
     4801 attgccggca ccgccgcgcc gtgggccatc aacctggccg ccgtcggcga agccgccgcc
     4861 ttcaacgccg atggctacaa ggccctcgtc tgcatctttc tgtacggcgg caacgacaac
     4921 ggcaacaccg tggtgccgta cgacaccagc ggccacgccg aatacctgac cgtgcgcggc
     4981 gcgctggcca cggcgcgcgc caatctggcc gccacggcgc tgaccggcgc cggactgccg
     5041 gcggggcgcc agtacgcgct ggcaccgcag ctgacgggcc tgaaagcctt gttcgacaag
     5101 aagcagctgg cggtgcagct caacgtcggc ccgttgctgc ggccgaccac gctggcgcag
     5161 ttccaggccc gctcggtgcc gctgccgccc aagctgtttt cgcacaacga ccagcaatcg
     5221 gtgtggcaaa gctcgcatgc cgaaggctcg gtcaagggct ggggcggcaa cctcggcgac
     5281 ctggcgctgg ccggcaatgg ccactcgctg ttcacctgca tgtcggtcac cggcaacgcg
     5341 gtgttcctgt ccggcgacag cgccttggcc taccagattg gcgccggcgg cgccgtcagc
     5401 atcaacggcg tgaccagcac cgacggcatg tacgggtcgg ccgcctgccg cacggcgctg
     5461 aaggatctga tcacccaaac ccgcccccag gtgctggaaa acgagctgaa cctcgtcacc
     5521 agccgatcca tcacctcgca agcgcaattg gccggcgcgc tggacagcag cgccacgcag
     5581 ttcgacgcgc tgctgcccga caaggtgggc gatcgcacga tggcgctcaa ccgccagctc
     5641 aagatggtcg cgcgcctgat cgccgggcgc cagcagctgg gcctcaagcg ccaggtgttc
     5701 ctggtgtcgc tgggcggctt cgacacgcac gacaacctca acaccaacca cccggcgctg
     5761 ctggcgcacg tgggcaacgc catggcgggt ttccagcagg cgctggactc gttgggcgtc
     5821 ggccagcagg tcaccacctt caccgcgtcc gacttcggcc gcaccatgac gtccaacggc
     5881 gacggctccg accatggctg gggcagccac cacttcgtga tgggcggcgc ggtgaaaggc
     5941 ggcgccttct acggcagcgc acccagcgcg ctgctgaaag gcccggaaga cgtcggccag
     6001 ggccggctgc tgcccaccac ctcggtcgac caatacgccg ccaccatggc gcgctggttt
     6061 ggcgtggccg acagcgaaat gacgcagatc gcgccgaaca tcggctactt cgacagcgtg
     6121 aacctgggtt tcatgacgta gcgctgcggg gcccacggcg gcccggctgg ctgaagacgg
     6181 caggcggccg ccaaagcatg ggaaaatcca cgctttgccg gccgccatcg cgcggcgcgg
     6241 cgccaccgct cttcgcacaa tccatgtcgt ccaccctgca ccccatgctc aacgtggcca
     6301 ttcgggccgc acgcgccgct ggcgccatca tcaaccgcgc cgcgctggac atcgaatcgg
     6361 tgcgcatctc gcaaaagcag gtcaacgatt tcgtcaccga aatcgaccac gcgagcgaag
     6421 acgcgatcat cgacaccctg ctcaccgcct acccgcacca cggcatgcac gccgaagagt
     6481 cgggcaccac gcgcggcaac cccaaggccg atcacgtctg gatcatcgac ccgctggacg
     6541 gcaccaccaa cttcatccac ggctttccgg tctattgcgt cagcatcgcg ctggccgtca
     6601 agggccgcat cgagcaggcc gtggtgtacg acccgacgcg caacgacctg ttcaccgcca
     6661 cgcgcgggcg cggcgcctac ctgaacgacc gccgcatccg cgtcagcaag cgcacgcagc
     6721 tcaatcaaag cctggtcgcc accggctttc cgtttcgcaa gggcgacgac tttccgacct
     6781 acctgaagat gatgggcgac gtgatgcagc gcaccgccgg cctgcgccgc ccgggcgccg
     6841 cggcgctcga cctggcctac gtcgccgccg gtttcaccga tggctttttc gaaaccggcc
     6901 tgaaggtgtg ggacgtggcc gccggctcgc tgttggtgac cgaggccggc ggcctgatgg
     6961 gcaatttcac cggcgagacg ggcgatctgg aacaaggcga atgtctggcc ggcaacccgc
     7021 gcgtctatgc gcaattggtg caggtgctgc gtcaatactc tcgatacgat agcgccgagc
     7081 gcacgtcgga cgggcgcaag gagcagattt cgctcaagaa accggcgact tccacgaaga
     7141 acgacgacgc ggccttcgac gcctgggcca aagacgctgc cacagaagct gccgctgaca
     7201 gcgccgcccc tgccgatgcg gccagtgggg ccgatcacag cgacnnnnnn nnnngggagc
     7261 cgtgagcacg ccgcccgacg ccgcgcccgc cggcgtggcg cacccgcgcc cgatccgcag
     7321 ctttgtcacg cgcgccggcc gcaccaccgc cgcgcaggcc cgcgcgctgg ctgaactggg
     7381 gccgcgccac gtgctgccct acgacccgtc tactactgat ttggtagctg cttgcgctga
     7441 tccggcaagc gctgacggcc aaaatcgctc aaaaaccgtg ctggagatcg gcttcggcat
     7501 gggccacgcc accgcgcaca tcgcggcgct gatgccggac acgctgttca tcggctgcga
     7561 ggtgcacgag cccggcgtgg gcgcgctgct gaaaagcatc ggcgagcaga acctggacaa
     7621 cctgcgcatc gtgcagcacg acgcggtcga ggtgctgcgc cacatgatcg cgccggccag
     7681 cctgcacggc gtgcacatct tctttcccga cccgtggcac aagaagcgcc accacaagcg
     7741 ccgcctgatc cagccgccct ttgtgcgcct gctggccgag cgcatcgccc ccggcggcta
     7801 cctgcactgc gccaccgact ggcagccgta tgccgagcag atgctggccg tgctgggcgc
     7861 cgagccgctg ctgaccaaca ccgccgccga ctacgcgccg cgccccgact accggcccga
     7921 gaccaagttc gagcgccgcg gcctgcgcct ggggcacggc gtgtgggatc tggtgtttcg
     7981 ccgcaacgat cggccagcga cgccgaatgc tacttagttg atagctgctc gcgcttgatt
     8041 ggcaagcgct ggcggtcatt ttcctccaaa tcatgagccg gccgccgcgc ccatcgcact
     8101 tgccgctgcg cgatggcgtc agcgccagtt gcgtggtggt gcgccccggt gatgcaccaa
     8161 cagcgctgga ttttctggcc gctcgcctag cggtgctgag ccgcgatgaa tgggccgagc
     8221 gtctggcgcg cggcgaagtt ctcgatgcgc agggccagcc gctgaacgcc agcacgccct
     8281 gccaccccgg ccagcgcatc tactactacc ggcgcctggc ggcggagccc gaaccgctgg
     8341 tcagcgagca catcgcgttt caggacgagt ggctggtggt agccgacaag ccgcacttca
     8401 tgccggtcac gccgagcggc cgctttgtgc agcaaagcct gctggtgcgg ctcaagcgcc
     8461 ggctgggcct ggccgatctg agccccgtgc accgcatcga ccgcgagacg gccgggctgg
     8521 ttgtttttgc cgtgcagacg cacacgcgcg atgcctatca ggcgctgttt cgggatcggc
     8581 gcgtgcacaa gctgtatgag gccgtggcgc cgcatcggtc tgatctggcg ctgccctgca
     8641 cccaccgcag ccgcctggcg cccgatgccg agcgcttttt tctctcgcgc gaagtggccg
     8701 gcgagccaaa cagcgaaacg cacatcgagc gcgcccagcc gctgggcacc cacgcgctgt
     8761 atcacctgcg ccccgtcacc ggccagcgcc accagttgcg gctgcacatg atggcgctgg
     8821 gcgtgccgat tctgggcgac ggcttctacc ctgaggtgct gcgcggcccg ggcgagccgg
     8881 acgatgcaga gaacccgctg caattgctgg cgcgcgcgct ggcgttcgac gatccggtga
     8941 cgggggagca gcgtcggttc agctcaagcc tgacgctgcg cgcggcgcag gcggcttcgc
     9001 ccgcgcccgc agcaggcgcc gatcaattcg acgacaaggt gccgaggttg gtcatgggcg
     9061 ccagcggcgc gtagtcgctg tcggcgcgcg cctgccggcg ctcgggggtg cgctggcgca
     9121 cggtcgtgcg caggtcggcc agcagctgtt cagccggcgc atccgcctcg gggcgctggt
     9181 tggcggcggc gatcagggtg gcaatgcgcg ccgacgagct gtcggtgcgc gcgcgatgcg
     9241 ccaggctctg ctcctcgccg tcgcggatca gcagccgaaa cggctcgccg cgcagctcgc
     9301 ccagctccat cagcttgcgc cggaagtact gttgcatctc gtcgcgcgcc gccgctggca
     9361 ccacctgcgg cgtctggatg accagcaaga ccccgtcgcg gccatcggcc gtgcgcgtag
     9421 cccagcactc ggcgcggccc accaggccga cgtggcgcat gacgtacagc gtggcgctct
     9481 gcaagcccgc agcgtggtca agggcgactg gaagcggtgg cgaattcgtt ggataaactg
     9541 catggcaaac aagggctggc gcccaagtgc acggcatagc gccaagaccc ttgttcaggg
     9601 ggattcgccc gccggcccga tagcttttgt cacgtttttt gcttttcaaa cacctgatgt
     9661 ccgcgctacc cgaaaacctc atcctgcacg agcgcggctg gctgtcgtcc aacaacctgc
     9721 tgtgcctggg cgacgcgccg gcgctgatcg acaccgggca cgtcaagcac gcggacgaaa
     9781 cggtgcggca ggtgcggcag gcgctgggcg accagccgct ggccgacatc gcccacacgc
     9841 acctgcacag cgaccattgc ggcggcaccg gcgcgctgca ggccgcgtgg ccggcggcgc
     9901 gcacctgggt gccggcgcca tcgctcgcgc atgtgcaggc gtgggacgaa gacgccctca
     9961 ccttcggcga cacggcgcag cgctgcgccc gctttgtcgc ccagcacgcc ctggtaccgg
    10021 ggcagacggt acggctgggc gacttcgact ggcagatcca cgcggcgccc gggcacgacg
    10081 cgctggccgt gctgctgttc gagcccacgc atggcgtgct ggtggccggc gacgcgatgt
    10141 gggagcacgg cgtgggcgtg atctttccgc acatcgatgg caccgacggc tttgacacct
    10201 tcaccgacac gctggcgctg atcgaacgac tagatccggc cgtggtggtg ccgggccacg
    10261 gcgccatctt cgagcgccag ggcggcgcca tcaccgccgc tattgcgcgt gcgcgcgccc
    10321 gcatcgacca gttcaacgcc aaccctgccg cgcacgcgct gcatgccgcc aaggtgttga
    10381 tcaagtacca gatgatggat gtggaacgca tgccgcgcgc ggatttcgag cgctggctgg
    10441 acagcgcccg cgcgctgcac gcgctgcacc agcagcaccg gcccgacatg gtttggcgcg
    10501 actggctggc gcacatcctg gcgccgatgt tcggcaaggg cgtgctgcgg caagatgcgt
    10561 acacggtgtt cgacggcgct tgagcacccg tcgctttgcg cgcgcagacg tcaggcgcgc
    10621 tttagcgccg accacagttc gccagccgtc atcaccggcg gcgcttcggg ccagcggcca
    10681 tccagcaatt gcaccatgcg cgcgttgcgc ggcgcggcca ggccatgcgc ccgcgccagc
    10741 cgcaccacct cgccgcacag cgcgtcgatc tcggtgcgac ggcccagcgc cacgtcgtcg
    10801 gccatgctcg atcgcgcttt ctcgtcgatg cgcagcatgc gcgctgccac gcgggtgaac
    10861 agtgcgtccg gcaggcgcag cagcgtgggc agccagcgcg gcggcaccgg cgccacgcgc
    10921 gccggctcga tgcccgccgc gcgcaacacg cccagcgcct catccatcaa cgccgcaaag
    10981 cagcgccgcc agtcgcgctg catcagctcg gcgcgcagcg gcaggcgcga cagcgcattc
    11041 accgggttgt tcaggttgag cagcagcttg ccccactgaa ccggcgccag atcggtgtgc
    11101 agcgccagcg gcaggcccgc gcgcgcgaac aggggcaacc agggggccaa cgccacatcg
    11161 tgctgcgcgg ccagcacccc ttgcgtgccg cggtgcagat gggcgggcgc cagctcggcc
    11221 acattgaagg gcaccatgcc cggcacgacg cgcaactgcg acgcaacggc ttgcgcgcgt
    11281 gccgcgttgt cgatgccgtt ttgcagcgac agcagcggcg tgcccgcggg cagcacgcgc
    11341 gccagttgcg gtcgcggcct cggtgccggc gctcttgacg gcgagcaaca ccaggcccgg
    11401 cgcagcgccg tcgggcggca gttgggacgc atccagcgca tccggcgcca cgtgcaacgt
    11461 ggggccttcg acgtcgctca gcgtcaagcc ttcggtgcgc agcacattca ccacgcgcgg
    11521 ccgaccgatg aagtgcacgc gcgcgcccgc cgccgccagc cggccaccga cccagcagcc
    11581 gaccgagccg gcacccatga cccacaccga ttcagcgtgc tgttcgccca cgttcacaga
    11641 tcaaatcccc gcctggcgct tgccagacag gcgacggcag ctatgctatc gatagtcatg
    11701 cagcgacctg ccgcgccgtg atctggtact tgaaatcgtc cggcgcgtag ctgtcgaagc
    11761 gcccatcggc gttctcggcc accgggtaca tgaagaagcc cagctcgccc tcctgcgcgc
    11821 ccggcaactt cacgtcgtgc gcggtgttgt aggccatcca gttgccttcc cagccgccga
    11881 acaggccctc gtacaccggc ttcacgatcg ggttgcccgc ctgcttgatc cactcccgcg
    11941 tttcatggcg catcaccttg gccacgtcgg ccgggtccat cgccgtccag ccgcgtgctt
    12001 gcaggtagac ctcggcgcgg cagtgctgcg cgcccttcag gctggtcggg ttgccgccca
    12061 gttcgcggta gccaaacgcc gagggcgcca ggcgaatgcc gtacacgtcg cgcgccggca
    12121 gccccacgct gcggcacagg ccgacgaaca gcgcgttcag gtcggcgcac ttgccgccca
    12181 ggttgccggt ctccagcatg gtcttgatgt cgccctcgcc gcagccgcgc accttgggtt
    12241 cgcggtaggc gttggccacc acccagtcgt agatggcgcg tgccttggct tcgtcgccgc
    12301 gcgcgccgcg cgtggcttgc cgcgccgtgt cgcgcacgac gccatcggtg ggcagcaggc
    12361 gcgtgggctt cagcgcctcg cgcaacgtcg cgctgtcttc cgccgcgcgg cggcgcgcct
    12421 gcgattcgtc gcggctttgc gtgcgcacgc gactggtcac ttcgacaaac ggctgcgccg
    12481 cgccgggagc aaattccgct gccaccatgc gtacgccctg cgcgccatcg ctggccaagc
    12541 gcgcctgccc gttgctgccg aaggtgtggc ccagcgtctg ctgccacggc gtttgcaccg
    12601 acggcagcgg cacccacacg cgcgtggcgc tggtggtggc gggcaaatcg atgcgggtgg
    12661 taacctcgaa cgtgcgccag tctcccggtt cggggtcgaa gcggcgcgct gctttggtgg
    12721 gggttgaggt ttgcgccagc aaggcgcccg gcaatgcggc ggcggcgccc aggccggccg
    12781 cgcgcccgat gagggcgcgt cgagtcgtgt tcatgaaaga gaactccgaa tggtgatgaa
    12841 cacccgtcgg cgcacacgag gtggcggcac gtggcgcgca accaatgccc cgaaggcggg
    12901 cggccggcgc gcacagtgcg cgaggcgagc ggattatcgc gccggctggc gcagcgcccg
    12961 cagccagccc agcgcttcgc tgccgctcca atccaccgcg ccggtgacga actggcgcgg
    13021 cgtacccgtg gtgtcgatca acaaggtgct cgggtagacc acaatgcccc aggccttcgc
    13081 gatcacgccc ttggggtcgg gcaggatggg cagcgtcagc ccggtggatc gggcgtagcg
    13141 ctgcacgtgg ggaatggatt cgcgcacgtt cacggtgagc agcaccaggt ccgcctcgcc
    13201 caactcgtgc agtgtctgca gcgtgggcat ctcttccttg catggcgcgc accaggtggc
    13261 ccagaagttg agcagcaccg actggccctt cagctcggcc agattccact cacggccgtt
    13321 cagatcggtg cccttcaggg gtggcatgga gctcgatcgc cgccaggtct tgatgtcgtg
    13381 ctgcgaaaac gagatcgagg ggagcatggt caggccggcc acagccagca gtgtggcgcg
    13441 ccgccgcgat agtgctggcg cgtcgtccag tggcccctgg ccgtcgcgcg atctaatcac
    13501 acccgctccg ccacccagcc ctgcacgctc tgcaaggcct tgggcaaccc ggacgcatcg
    13561 gtgccacccg ccattgccat gtccggcttg ccgccgccct tgccgcccac ttgctgggcg
    13621 acgaagttga ccagctcgcc ggccttgact ttgccaatcg catccttggt gacgccggcg
    13681 gccagttgca ccttgtcgcc ctccacggcg gcgagcacga tggcggcggt cttcagcttg
    13741 tctttcagct tgtccatggt ctcgcgcagt gtcttggcgt cggcgccttg cagcgtggcg
    13801 gcgagcactt tgaggccatt cacatcgacg gcttgattca ccagttcatc gccttgcgat
    13861 gaggccagct tgcccttgag ctgcgcgatt tctttttcga gggccttcac ctgatcgagc
    13921 acctggccca gccgcgcctg cacctcggcg ggcggcgtgc gcagcgtggc ggccacgctg
    13981 tgcaggctgg cttccaggcc ttgcgtgtat tcgagcgcgt tggcgccggt gatggcctcg
    14041 atgcggcgca cacccgccgc gacgccgcct tcgctgacga tcttgaacag gccgatgtcg
    14101 cccgtgcgcc gcacgtgtgt gccgccgcac agttccttgc tggagccgat ggtgagcact
    14161 cgtacggtct cgccgtactt ctcgccaaac agcatcatgg cgccggtttt ctgcgcgctg
    14221 tccaggtcca tgaccgacgc atcggtcgcg gcgttggcca gaatttcttc gttcacccgg
    14281 cgctcgatct cggtgatctg ctcgggcgtg acgggcgcgt tgtgcgcgaa gtcgaagcgc
    14341 gtcttctcgg catcgaccaa cgagcccttc tgctgcacgt gcgcgcccag cacctcgcgc
    14401 agcgccttgt gcatcaggtg cgtga
//
LOCUS       DAOMVO010000026        14335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_925_length_14335_cov_19.066457, whole genome shotgun sequence.
ACCESSION   DAOMVO010000026 DAOMVO010000000
VERSION     DAOMVO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14335
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_925_length_14335_cov_19.066457"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKV30_02665"
     CDS             <1..417
                     /locus_tag="PKV30_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HRL29154.1"
                     /translation="VPHKGDVAGSVIEGAFEVLSGFERVKESRDLMRAITLDEGEAEV
                     FARSALALKYDPTDNKPAPITESQILMPRRFDDRRPDLWSVFNRTQENLTKGGLHGRS
                     ANGRRQQTRPVQGIDSDVRLNRALWMLADGLRQLKA"
     gene            498..2564
                     /locus_tag="PKV30_02670"
     CDS             498..2564
                     /locus_tag="PKV30_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRL29155.1"
                     /translation="MNAITQTEARALNTAAAIPLEAADPTKNLILVPLSRLVSRPAGR
                     NVRKTPRMSIPELAASIQRVGLLQNLIVIAAADGEHYEVVAGGRRLAALKLLAKKRRI
                     SKEWEVPCLLVADGTARTASLTENVQREAMHPADQFEAFAALVAEGRPIEDIAADFSV
                     TPLVVQRRLKLANVSPRLLADYRAEAVTLDQLMALAITDDHAAQETAFYDAPTWQRNP
                     SQLRDRLTEREIDAYRHPLVRFVGLDTYGAAGGGVRRDLFAEGDAGVYLTDAALLERL
                     AQDKLAGIAAEVKDEGWAWVDATPAVTHADLHAFQRAPRERREPNKREAQRIEKLQTK
                     MHELAAAVDDALDTEDEEKADALQEEGEAVGEQLQALEDGLQDYGTTVKAAAGAIVTI
                     DRNGQAVIHRGLMREAEAKALRTLERLRQGFGSESEAGNDDEGEDDEQPKTAAMSDKL
                     AQRLSAHRTAALQIEVARHPQVALAALVHGMVQTVLQGSHYGHDLPLGVSLKAQDRLE
                     GIAPDIPDSPAAVALRELQQVAGEGLPEDSAELFAVLLAKSQDELVRLLAISVAVTVD
                     VVTPRATPQQPGAELAQAVGLDMAAWWKPTNEGYFRHVPKAAILEAVEQYAPAHVTRL
                     AKLKKADIASEAERLADGTGWMPAIFKAEGTDDTTAQDAPQAVTNDEPEAIDNDDEQQ
                     AHALAA"
     gene            3375..3689
                     /locus_tag="PKV30_02675"
     CDS             3375..3689
                     /locus_tag="PKV30_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HRL29156.1"
                     /translation="MANIGTFTAEKDGFTGQLRTLTLNFKVKLVPNDKGENESAPDFR
                     LQAAGHDFGAAWKKTSEAGRDYLSVAIDDPSFPATVYARLIEGENDTHDLIWSRSKPK
                     AA"
     gene            complement(3992..4285)
                     /locus_tag="PKV30_02680"
     CDS             complement(3992..4285)
                     /locus_tag="PKV30_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL29157.1"
                     /translation="MTAKHSLATAIRTVRKARGLSQEAFSDVSSRTYMSTLERDLKSP
                     TLHKLTELCEVMEVHPLTLLTLAYAGDDTHKADELLARVRQEFEAVLKERDAP"
     gene            4404..4955
                     /locus_tag="PKV30_02685"
     CDS             4404..4955
                     /locus_tag="PKV30_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HRL29158.1"
                     /translation="MRIPTEPGNRIRVFPASDNSASALLFFYGCDGLHIGASTRMHGC
                     VSAVLRFGTKAHSRIVGFVEKLPMTLPLVTADERARLLAHGAALAAGQRLDPLPVVRL
                     FTPDAHVTWLLVSLDPADGDTAYGLIDLGIGMPELGMVKLSDLAGIVGPRKLPVRRDR
                     YFQAVRPLSEYVRLAQENGAITD"
     gene            5299..6066
                     /locus_tag="PKV30_02690"
     CDS             5299..6066
                     /locus_tag="PKV30_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HRL29159.1"
                     /translation="MTDRSAEHWYPTAAYLYVLHLDGPALAWEYLRRNPDYRRDWLRR
                     RRRPDTAQAWGLRLLEDPALDARDAHPAWFPDHDAVVQVYPDDDPPPEAHAFEFWRVP
                     GRKQLIHDGKRLVLVSHWPGCCLRLALAPGLEDGMAYLYATRACATPCARYRTLASEL
                     DALAVATVATPAAAARSRPTTAAVLELHTLQTLDATLAGASLREVAEGLFGADAVAAD
                     WHKDSALRARVRRLVRRGDALMRGGYRRLAQLPPPLQ"
     gene            6179..6463
                     /locus_tag="PKV30_02695"
     CDS             6179..6463
                     /locus_tag="PKV30_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL29160.1"
                     /translation="MRPAPLRPAAAAVAAPAQPQRYLTNDEAAEYLRLSPRTLEKQRV
                     IGGGPKFRKFGRRVMYAVSDLDAWADQRSYEATSDPEYAERHAGDYRDGR"
     gene            6490..7335
                     /locus_tag="PKV30_02700"
     CDS             6490..7335
                     /locus_tag="PKV30_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HRL29161.1"
                     /translation="MSSPPGQALPQREQLDLFRALPGDMAPRDSQDLMAFPFFSLAKS
                     RRTAPIDFRAGGITIRVEGTAEHGIATIWDADVLIWAASQIVEARDAGLRPSRWIRAT
                     PYEILRFIGRGTSLNDYLRLKAALDRLQSTTVATSIRETTGRRLHRFSWINEWKELAD
                     ASGVPLGIELILPDWFYAGVLDAALVLTIDPAYFRLKGGIERWLYRLVRKHGGKQPGG
                     WQFDFRHLHRKSGSAARFSDFAYDLRVLVARQSLPGYVLGIERMPDDGQELLTFRPVL
                     HTARG"
     gene            7601..8239
                     /gene="parA"
                     /locus_tag="PKV30_02705"
     CDS             7601..8239
                     /gene="parA"
                     /locus_tag="PKV30_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HRL29162.1"
                     /translation="MILALLNQKGGVGKTTLATHIAGELALRGQHVVLLDADPQGSSL
                     DWTQRRSQQGLPRLFSAVGLARETLHQEAPELARRADHVIIDGPPRIAALARSALLAA
                     ERVLIPVQPSPYDLWASSEMVSLIREAQVFRPLLRAAFVINRRVSTTIIGREARQSLA
                     EQPLPALRSEVHQRIVFADSVAAGRLARETAPDSAAAREITALVDELLRWPT"
     gene            8236..8511
                     /locus_tag="PKV30_02710"
     CDS             8236..8511
                     /locus_tag="PKV30_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HRL29163.1"
                     /translation="MTAQQPPNSKRTGKRVGIGARPPANPHAEAWIRQGDADALGKGD
                     LYTARLTLDITPAMRARIKVSAFTQGVTVADLLRGLLEREFPPENTP"
     gene            8508..9053
                     /locus_tag="PKV30_02715"
     CDS             8508..9053
                     /locus_tag="PKV30_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005304564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HRL29164.1"
                     /translation="MNAFAWPAANAAPAAPLPALSALAGQAGNVPLTRVSLAYLEHRF
                     KLYLRFGEPARTLQLDRWRRCAVFLPGAMLCRIRWQANDYGTIRWQLMVMQACPPLDA
                     AQRIPGVQPGARLLLHAEGDANVRAVLERIDGIEALGIAAIDVSPAYWRTLGNRLAAR
                     LALPEYTTERHTAWLAGKVLP"
     gene            9050..9649
                     /locus_tag="PKV30_02720"
     CDS             9050..9649
                     /locus_tag="PKV30_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRL29165.1"
                     /translation="MTAPTTTPNAPDVAPRPRSRLRARIVLATLAAIGFAALAWAAFV
                     SPLPRLTYNPSDSVAVGWYRIDPFDPRTASLPRPLSVDSIVLVPLPATAAALAAQRGY
                     LPTRVPLLKRVGAVAPQHVCIVAGQVRIDGVPSAAVLPADRLGRALPSLQLCRRLEPG
                     ELLLLSVTNPASFDSRYFGPVSASAVIGVAHPVWPEIRP"
     gene            10086..12068
                     /locus_tag="PKV30_02725"
     CDS             10086..12068
                     /locus_tag="PKV30_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease and DUF3363
                     domain-containing protein"
                     /protein_id="HRL29166.1"
                     /translation="MTDSRDDDFRVRPSAPKNRGKGQGQSFVSKVLKQAGKASSGKSA
                     VRRPGAAGTGQRPGSRLGRGHTAARFAGAKLMPMSRRVTIKTLLVNHQRASPQSLAKH
                     LRYIERDGAGRDGEPGRGYGPQADEADLDAFKERCADDRHHFRFIVSPEDGAELDDLR
                     TYTRHLVSRMEADLGTRLDWVAVDHWNTDNPHTHLIVRGRDDTGKDLIIAGDYIADGF
                     RHRAAELATEWLGPRTELEIQQTLRREVEQERWTSLDRTLQRETGEDGRVHVERLNEP
                     RLQRQRLLLIGRLQRLQRLGLADEVQPGTWAIHADAEKTLRALGERGDIIRTMQRAMS
                     GQPRELAVFEPGDDGRSLIGRVAAKGLADELHDRGYLIIDGTDGKAHYVALNARDELA
                     NYPTGAVVEVKGSTEVRTADKNIASLASDGLYRTDHHLVIEQGRATPGRDPQEVVAAH
                     VRRLEALRRACIVERVAEGLWKVPDDLAERGRQYDAQRLGDVAVELKSHLPIERQARV
                     IGATWLDQQLIGGGRGLGNLGFGGDTKQAIQQRADFLEEQGLAQRRGQRVILARNLLG
                     TLRNRELVQAAKDIAAETGLEHRPVADGQRVAGIYRRSIMLASGRYAMLDDGMGFSLV
                     PWRPVIEPRLGQQLAAKVIGGSASWELGRKPSIGIS"
     gene            complement(12061..13554)
                     /locus_tag="PKV30_02730"
     CDS             complement(12061..13554)
                     /locus_tag="PKV30_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRL29167.1"
                     /translation="MPAFLVLVYSKRCVVLLAEVKRLEVCKFTRWSLAGVLATVVLTG
                     CSTYRPWINPTLSDAALMSLAENDQRIRPSEVNQPVVVAVTLSGGGARAAAFGLGVLR
                     ELKATRFALEGQETTLLDQVFLVSGVSGGSILAAHFAAFGDATLTRFEPDFLLVPFEG
                     RLIREALWPERLYRLTSPWFGRSQILAQRLDELFEGRTFGDVRRRPGAPELMITATDL
                     TTGAPFDFTTEQFRLICSDLDATPLSFAVAASSAVPLVLSPVTVRNNANTCPPPLRSL
                     PVRGQNYRSRILQSGIDSYLNAHDRPYIHLVDGGVSDNLGVRLMLDRLVASGSMSANF
                     SDVKPGSLRRLVLVTVNSERGLSERVDSSDRVPSTTQVLESLIFGAGARETQVTLAML
                     NDDLQHWRSEIVRTRGQADSPFAADAEIYVVSVSLNDVPDDKIRHSLLRVPTAFTIEA
                     TDVLDLQTAGGEALRQSKEFMALIESLRRITAAKQTAPGTQLPLISN"
     gene            complement(13671..14288)
                     /locus_tag="PKV30_02735"
     CDS             complement(13671..14288)
                     /locus_tag="PKV30_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HRL29168.1"
                     /translation="MKRPISLIHFVLLAGLTATLSGCASNGMTRSGFLGDYDVLGKTK
                     YENVLLFKAPGFEPSRYREILVEDAQVVTATGRIGGLEAEQVRELLDHVNSELRHLQT
                     KSEAPASSGRIRVRVAITTIETPNRAVNTLTTLLVGPVTTGGASLEFEAVDEQTDRRV
                     AAASCFEHGNALADFKGSYTLLGHAKSAITTCLERINSAWRSAGS"
BASE COUNT         2566 a         4763 c         4583 g         2423 t
ORIGIN      
        1 gtgccgcaca agggcgacgt ggccggttct gtcatcgaag gcgctttcga ggtgttgagc
       61 ggcttcgaac gcgtgaagga atcccgcgac ctgatgcgcg ctatcacgct ggacgaaggc
      121 gaagccgaag tgttcgcccg ttccgcgctg gccctcaagt acgaccccac cgacaacaag
      181 cccgcgccca tcaccgaatc gcaaatcctg atgccgcgcc ggttcgacga ccgccgcccg
      241 gacttgtgga gcgtgttcaa ccgcacgcaa gaaaacctga ccaaaggcgg attgcatggc
      301 cgcagcgcca acggacgccg ccagcagacc cgccccgtgc agggcattga ttccgatgtg
      361 cgcctcaatc gcgccctctg gatgttggcc gatggcctgc gccagttgaa ggcctgattc
      421 cccacgcggc aggggcaggc agcagccctt gccgctttct tcgctgctgc atccctgaac
      481 cgataggagt tatcaccatg aacgccatta cccaaaccga agcccgcgcc ctcaacaccg
      541 ccgccgctat cccgctggaa gccgccgacc cgaccaagaa cctgattttg gttccgctgt
      601 cgcggctggt gtcgcgtcct gctggccgca acgtgcgcaa gaccccgcgc atgtccattc
      661 ccgaactcgc cgcgagcatt cagcgtgtcg gcctgctgca aaacctcatc gtcattgccg
      721 ccgccgatgg cgagcattac gaagtcgtgg ccggtggccg caggctggcc gcgttgaagc
      781 tgctggcgaa gaagcgccgc atcagcaagg aatgggaagt gccttgcctg ttggtggccg
      841 atggcaccgc ccgcacggcc agcctcaccg agaacgtgca gcgcgaagcc atgcaccccg
      901 ccgaccagtt cgaggcattc gccgcgctgg tggccgaagg ccgacccatc gaggacatag
      961 ccgccgattt cagcgtcacg ccgctggtgg tgcagcgccg cttgaagctg gcgaacgtct
     1021 cgccccggct gctggccgac taccgcgccg aagccgtgac gctcgatcag ttgatggccc
     1081 ttgccatcac cgacgaccac gccgcgcagg aaaccgcgtt ctacgacgcg ccgacgtggc
     1141 agcgcaaccc ttcccaattg cgcgaccgcc tcaccgagcg cgagattgac gcataccggc
     1201 atccgctggt gcgtttcgtg gggctggata cctacggagc cgcaggcggt ggtgtgcgcc
     1261 gtgacctgtt cgcggaaggt gacgcgggcg tgtatctgac cgatgccgcg ctgctggaac
     1321 ggctggcgca agacaagctg gcaggtatcg ccgccgaggt gaaggacgaa ggctgggcgt
     1381 gggtggatgc cacccccgcc gtgacccatg ccgacctgca cgccttccag cgtgcgccga
     1441 gggagcgccg cgaaccgaac aagcgcgaag cgcagcgcat cgagaagctg caaaccaaga
     1501 tgcacgaact ggccgccgcc gtggatgacg cgcttgatac cgaggacgag gaaaaggccg
     1561 atgccttgca ggaagaaggc gaagccgtgg gcgagcagtt gcaggcgctg gaagatggct
     1621 tgcaggacta cggcacgacc gtgaaggccg cagccggtgc catcgtcacc atcgaccgca
     1681 acgggcaggc agtgattcat cgcgggttga tgcgcgaggc cgaggccaag gcgctgcgca
     1741 cactggaacg cctgcgccaa ggtttcggca gcgaaagcga agccgggaac gacgacgaag
     1801 gcgaggacga cgagcagccc aagaccgccg ccatgtccga caagctggcg cagcggttga
     1861 gcgcccaccg caccgccgcg ctgcaaatcg aagtcgcccg gcatccgcaa gtggcgctgg
     1921 ccgcgctggt gcatggcatg gtgcagaccg tcttgcaggg cagccactac ggccacgact
     1981 tgccgctggg cgtgagcctg aaagcgcaag accggctgga aggcatcgcc ccggatatac
     2041 cggattcgcc cgccgccgtg gcgctgcgcg agttgcagca ggtagcaggc gaaggcttgc
     2101 cggaggacag cgccgaactg ttcgccgtgc tgctagcgaa gtcgcaagat gaactggtgc
     2161 ggctgctggc gatatccgtg gccgtcacgg tggacgtggt gacaccccgc gccacgccgc
     2221 agcaaccggg cgcggaactg gcgcaggctg tcggcttgga catggccgca tggtggaagc
     2281 ccaccaatga gggttatttc cggcatgtgc cgaaggccgc gattctggaa gccgtggagc
     2341 agtacgcccc ggcgcacgtc acccggctgg cgaagttgaa gaaggccgac attgccagcg
     2401 aagccgagcg gctggccgat ggcaccggct ggatgcctgc catcttcaag gccgaaggca
     2461 ccgacgacac cacggcgcag gatgcgccgc aggcggtgac gaacgacgag ccggaagcca
     2521 tcgacaacga cgacgagcag caggcccacg cactcgccgc ctgaccttca ccaccaccga
     2581 gcgccccggt ttcggccggg gcgcttcgct gttccatcag catcggcccc aggccgttcc
     2641 gccctggtgc cgatggtcaa aaatccgggc gcggcggtgg ccgcgcccgg tttccaagcc
     2701 caaagccaga acgccccttc gccgccttcg ggcggacggc gaaggggcgg cgcacaagtg
     2761 accttgtgcg cgggaaaccc tcgaagccca taagtgcgtc ggcgcacagc gccgacgaca
     2821 ttccgaacgc gccccaccgc aatggacggg cgtgtggggc tacgccccgg cttgctgccg
     2881 caggttgcac gaaagcgtgc cgtcctcgac gctggcggtt cgtcgcctgt acgtcacgaa
     2941 ggacggttca ccgacacgcc gcccggcctt gcaatggagc ttccacgcca cgcctcacgc
     3001 aaagggccgc aagcagcagt agggacgctt tcaccttgtc gctgggcatt gtccttgccc
     3061 gtgtcagggc gcaggccggt gaagccgtcg cgcggggatg ccgagtcggc catgcctcca
     3121 ttcgggagcg ggcggccagt acgtccttgt gttgttgtga atcctggcgg tggctgggct
     3181 tcatggccgc aaccttccag cgaaaacaat tccccctgcg ctgcgcgcat tcctcgcggg
     3241 acaaattctt ttcgcttcca ggttctccac ggtgttgcga ccgctgcgcg gtgcggccag
     3301 cccatccccc gccggacgga tcacaacaag gacgcactgg cgcgaccttg ttcaacccga
     3361 aaggagaaat catcatggct aacatcggca ccttcaccgc agagaaagac ggcttcaccg
     3421 gccagctccg caccctgacc ctcaacttca aggtcaagct ggttcccaac gacaagggcg
     3481 agaacgaaag cgccccggac ttccgcctgc aagcggccgg ccacgacttc ggcgcggcgt
     3541 ggaagaaaac cagcgaggcc gggcgggatt acctgtcggt ggccatcgac gatccttcat
     3601 tcccggcgac ggtctatgcc cgcctgatcg aaggcgagaa tgacacgcac gacctcatct
     3661 ggtcgcgcag caagcccaag gcggcctgac ggccgcccac cgcgccccgc ccattgcggc
     3721 ggggcgcggt gctgctgatc gcagcatcgc acgcacaggg tttcggcagt tgccccatgt
     3781 cccgcatgtc caaggccagc attgcattgg tgatggtcga atggctcgaa gcccgtggca
     3841 gtaggaacag catggcgtgt cggcgcattg cgccgccggg ctttcgggct gcgccccatg
     3901 ccgccaatgg cgtcatgtcc attcggcttc aatccctcac gccttcgcgc ctggcggcgc
     3961 tgcgcgctcc gcttgcgggc attcagacgc gctagggcgc gtcgcgctct ttcagcacgg
     4021 cctcgaactc ctggcgcacc cgcgccagca gttcgtcggc cttgtgcgtg tcgtcgccgg
     4081 cgtaggccag cgtcaacagc gtcagcggat gcacttccat gacctcgcac agctcggtca
     4141 gcttgtgcag ggtcggactt ttcaagtcgc gctccagcgt actcatatag gtgcggctgg
     4201 acacgtcgga gaacgcttcc tggctcaagc ctcgcgcctt cctgactgtc cgtattgccg
     4261 ttgccaatga gtgtttcgct gtcactaact tggtttcccc ataaaaacca agatgacagc
     4321 ccattgcgcc ctatagggct acaatctata gtgttcattc atggctgaaa ggcccgcttt
     4381 cgtgctttta cggaaatccg cagatgcgga ttccgacaga accggggaat cgcatccgtg
     4441 tctttccggc ttccgacaat tccgcttcgg cgcttctgtt cttctacgga tgcgatggct
     4501 tgcacatcgg tgcttccaca cgaatgcacg gatgcgtttc ggcagttctg cgcttcggca
     4561 ccaaagcgca ttcgcgcatc gtcggtttcg tggagaagct gcccatgacc ctgccgcttg
     4621 tcaccgctga tgaacgcgcc cggctgctgg cccacggcgc ggcgctcgcc gctggtcagc
     4681 ggctcgaccc gctgcccgtg gtgcgcttgt tcaccccgga cgcgcatgtg acctggctgc
     4741 tggtgtcgct cgacccggcg gatggcgaca cggcctacgg cctgatcgac ctcggtatcg
     4801 gtatgcctga gctcgggatg gtcaagctgt ccgacctggc gggcatcgtc gggccgcgca
     4861 agctgccggt gcggcgggat cggtatttcc aggcggtgcg cccgttatcg gagtacgtcc
     4921 ggctggcgca ggagaacggc gcgatcacgg actgatgaaa accagcgcgg cctagccagc
     4981 cggggcgcat cgtgactatt tcaatcttga tcaagaccgt gcaggtctga atccgcattc
     5041 ttgcaccgaa gtggtgcgat cctgttgaaa acagtcctga tcttgggcgc gggctgcggc
     5101 acggtgttcg gtgctgcgcg tcattttcct gaaggcgtag ccgtcgcatt cagacgattt
     5161 ccgcaacacg cagagccaaa tggtcttgac accgacagtt acagcctaac caattttgat
     5221 gacgacctga tggcatttcg atagttcccg catcgcggaa atcgtggcga cgcatcccaa
     5281 acagagggag gtttcgccat gactgatcgc agcgccgaac actggtatcc gacagcggcc
     5341 tatctctacg ttctgcacct tgacggccct gcgctggcgt gggaatacct gcgccgaaat
     5401 cccgactacc gccgcgactg gctgcgccgc cgccgccggc cggacacggc gcaggcgtgg
     5461 ggcctgcgcc tgctggaaga cccagccctg gatgcgcgcg acgcgcatcc ggcctggttt
     5521 cccgatcacg atgccgtcgt gcaggtttac ccggacgatg acccgccacc cgaagcccat
     5581 gccttcgagt tctggcgcgt ccctggccgc aagcaactga tccacgacgg caagcgcctg
     5641 gtgctggtgt cgcattggcc cggctgctgc ctgcggctcg cgctcgcgcc gggcctggaa
     5701 gacggcatgg cctacctcta cgccacccgc gcctgcgcca cgccctgcgc gcgctatcgc
     5761 acgctcgcgt ccgagctgga cgcattggcc gtggccacgg ttgccacgcc tgcggcggcg
     5821 gcccgttcca ggcccacgac ggccgcggtg ctggaactgc acaccttgca gacgctcgac
     5881 gcgaccctgg cgggcgcgtc attgcgcgaa gtggccgaag ggctgttcgg cgccgatgcc
     5941 gtcgccgccg actggcacaa ggacagcgcc ttacgtgccc gcgtgcggcg gctggtacgt
     6001 cgtggcgatg cgctgatgcg tggcggctat cgccgcctgg cacagcttcc gccgccgttg
     6061 cagtaggggg ggacgtttcg cgtcccccgt agatcgtccc ttagcaagca gcctcgcttt
     6121 cttgaaagtg cctctaaccg gccgcgtggt gtggccgggc ttgatggagg tacatcccat
     6181 gcgacccgct cccttgcggc ctgccgccgc tgctgtcgct gcgcctgcgc agccccaacg
     6241 ctacctgacc aacgacgaag ccgccgagta cctgcgcctg tcgccacgca cgctggagaa
     6301 acagcgcgtg atcggcggcg gcccgaagtt ccgcaagttc ggccgccgcg tcatgtacgc
     6361 ggtgtccgac ctcgatgcct gggccgacca gcgcagctac gaggcgactt ccgacccgga
     6421 atatgccgaa cgccacgcgg gcgattaccg tgatggccgc tgatcgctgg cgcgtgggtg
     6481 gccgtcgcca tgtccagccc gccagggcaa gccttgccgc agcgcgaaca gctcgacctg
     6541 ttccgcgcgc tgccgggcga catggcgccg cgcgacagcc aggacttgat ggcctttccg
     6601 ttcttctcgc tggcgaagtc gcggcgcacc gcgccgatcg acttccgcgc cggcggcatc
     6661 accatccgcg tggaaggcac ggccgagcac ggcatcgcaa cgatatggga tgccgacgtg
     6721 ttgatatggg ccgcctcgca gatcgtggag gcgcgcgacg cgggcttgcg cccgtcgcgc
     6781 tggatacgcg ccactcccta cgagatcctg cgcttcatcg ggcgcggcac gtctctcaac
     6841 gactacctgc gcctgaaagc cgcgctcgac cggctgcaat cgaccacggt ggccacgtcc
     6901 atccgcgaaa ccacgggaag gcgattgcat cgcttctcgt ggatcaacga gtggaaggaa
     6961 ctggccgatg ccagcggcgt gccgctgggc atcgaactga tcctgccgga ctggttctat
     7021 gcgggcgtgc tcgatgccgc cttggtgctg accatcgacc cagcctattt ccgcttgaag
     7081 ggcgggatcg agcgctggct gtaccgcctg gtgcgcaagc acggcggcaa gcagccgggc
     7141 ggctggcaat tcgacttccg gcacctgcac cgcaagtcgg gcagcgctgc gcgcttctcc
     7201 gacttcgctt acgacctgcg cgtcctggtg gcgcggcagt cgctgcccgg ctacgtcctg
     7261 ggcatcgagc gaatgccgga cgacggacaa gaactgctga ccttccggcc cgtgctgcac
     7321 acggcacggg gataactcgt ggaaaaggtg tggacggcct cgtgctatca ggagtaccgg
     7381 gtatcgtgct atcgggagta cgcctatcgt gctatcagga gtacgaaaac gccggaaagc
     7441 caataacggc gcgggtttgc ggcccctcta acttccctaa caagaaatac ataactttta
     7501 gtagaagcgc gccgtttcgg tggacaaccg agaaacggca cggcgaacag cgttttgcac
     7561 ggcaggtaaa gcccggcttt ccagcaggga gggccgcgcc atgatcctcg ctctgctcaa
     7621 ccagaaaggc ggtgtcggca agaccacgct cgccacgcac atcgcgggcg aactggcgct
     7681 gcgcggccag catgtcgtcc tgctcgatgc cgacccgcag ggttcatcgc tggactggac
     7741 gcagcgcaga agccagcaag gcttgccacg gctgttcagc gccgtgggcc tcgcacgcga
     7801 aacgctgcat caggaagcgc cagaactcgc caggcgggcc gatcacgtca tcatcgacgg
     7861 gccgccgcgc atcgccgcct tggcgcgctc cgcgctgctg gcggccgagc gcgtgctgat
     7921 cccggtgcag cccagcccct acgacctctg ggccagctcg gaaatggttt cgctgatccg
     7981 cgaggcgcag gtgttccggc cgctgctccg cgcggccttc gtcatcaacc ggcgcgtcag
     8041 caccaccatc atcgggcgcg aggcgcggca atcgctcgcc gaacagccgc tgcccgcgct
     8101 gcgctcggag gtgcatcagc gcatcgtctt cgccgacagc gtggccgctg gccggctcgc
     8161 acgcgagaca gcgcccgaca gtgccgccgc ccgcgaaatc accgccctgg tggacgaact
     8221 gctgcggtgg ccgacatgac agcgcagcag ccacccaaca gcaaacgcac gggcaagcgc
     8281 gtcggcatcg gcgcacgtcc gcccgcgaat ccgcacgccg aggcgtggat tcgtcagggc
     8341 gatgccgacg cgctgggcaa aggcgacctc tacacggccc gcctcacgct cgacatcacg
     8401 cccgccatgc gggcgcgcat caaggtgtcg gccttcacgc aaggcgtgac cgtggccgac
     8461 ctgctgcgcg gcctgctgga gcgggagttt ccgccggaga acacaccatg aacgcattcg
     8521 cttggcctgc cgcgaacgcg gcaccggctg cgccgctgcc tgcgctttcg gcactcgccg
     8581 gacaggccgg caatgtgccg ctgacgcgcg tttcgctggc ctacctcgaa catcgcttca
     8641 agctctacct gcgcttcggc gaaccggcgc gcacgcttca gctcgaccgc tggcggcgct
     8701 gcgcggtgtt cctgccgggc gcgatgctct gccgcatccg ctggcaggcc aacgactacg
     8761 gcacgatccg ctggcagctc atggtgatgc aggcttgccc gccgctggac gcggcacagc
     8821 gcatccccgg cgtgcagccg ggcgcgcgct tgctgctgca cgccgaaggc gatgccaacg
     8881 tgcgcgccgt gctggagcgc atcgacggca tcgaggcgct gggcatcgct gccatcgacg
     8941 tgtcgccggc gtactggcgc acgctgggca accgtcttgc ggctcgcctt gcgctgcccg
     9001 aatacaccac cgagcggcat accgcctggc tggccgggaa ggtgctgcca tgaccgcccc
     9061 gaccaccaca cccaacgcgc ccgacgtggc gccgcgtcct cgctcacgcc tgcgcgctcg
     9121 catcgtgctg gcgacactgg ccgccatcgg cttcgctgcg ctggcctggg cggctttcgt
     9181 gtcgccgctg ccgcgtctga cctacaaccc gtccgacagc gtggcggtcg gctggtatcg
     9241 cattgacccg ttcgacccgc gcaccgcctc gctgccacgt ccgttgtccg tggacagcat
     9301 cgtgctggtg ccgcttccgg ccacggctgc cgcgctcgct gcgcagcgcg gctacctgcc
     9361 gacgcgcgtt ccgctgctca aacgtgtggg cgcggtcgcg ccgcaacacg tctgcatcgt
     9421 cgccggtcag gtacgcatcg acggcgtgcc ttcggccgcc gtactgcccg ccgaccggct
     9481 gggccgggcg ctgccgtcct tgcagctttg ccgccgcctt gaaccgggcg aactgctcct
     9541 gttgagcgtg acgaatccag catcgttcga cagccgctat ttcggcccgg tcagcgcatc
     9601 cgccgtgatc ggcgttgcgc atccggtgtg gccggagata cgcccatgat ggccgccgat
     9661 tcgctgcgct ctgacgtgcc atcgggcatg tcgttctgct ggctgcttca gtgtcgctgc
     9721 gcctgtcgtg ccggtgcatt cgcaccgcac gtcgcgcagc atccggcgca gccgtccaac
     9781 gttcaggcgt cttgcctcga acgcgcccgg cctgcggcca ttggcgcttt cgccggcgcg
     9841 ctgtctgttc acgcagcagc gccgccgggc cgccgttgcc tggagcgaca gcgaagggca
     9901 aaggcggaag gcaagacaaa aggacgcggc acctggccgc gtcgaaagcc agtcagcaca
     9961 tggggggaac gcggcacgga gcggcttcgc caccgtgccg cgtttggcgc gagggtcgcg
    10021 ccaatgctgg catgtccgcg tgcttcgcac gacaggacac gcccaagctt gcggggagcg
    10081 cagccatgac cgacagccgc gacgacgact ttcgcgtgcg ccccagcgcc ccgaagaacc
    10141 ggggcaaggg ccaggggcag agcttcgttt ccaaggtgct caagcaggcc ggcaaagcca
    10201 gcagcggcaa gtcggcagta cgccgtcctg gcgcggctgg caccggccag cggcccggct
    10261 ctcggctcgg acgtggccac acggcggcgc gcttcgccgg ggcgaagcta atgcccatgt
    10321 cgcggcgcgt gaccatcaag accttgctgg tcaatcacca gcgggccagc ccgcaatcgc
    10381 tcgccaagca cctgcgctac atcgagcgcg acggcgcggg tcgcgatggc gaaccgggcc
    10441 ggggctacgg gccacaggcc gacgaagccg atctggacgc cttcaaggaa cgctgcgccg
    10501 acgaccggca ccatttccgc ttcatcgtct cgcccgagga cggggccgag ctggacgacc
    10561 tgcgcaccta cacccggcat ctggtgagcc gcatggaagc cgacctgggc acgcggctgg
    10621 attgggtggc ggtggatcac tggaacaccg acaacccgca cacccacctg atcgtgcgcg
    10681 ggcgcgacga caccggcaaa gacctcatca tcgcgggcga ctacatcgcc gatggcttcc
    10741 gccatcgcgc cgccgagctg gcgaccgagt ggctggggcc gcgcaccgaa ctggagattc
    10801 agcagacctt gaggcgcgag gtggaacagg agcggtggac gagcctggat cgcaccttgc
    10861 agcgcgagac cggcgaggat ggccgggtgc atgtcgaacg gctcaacgag ccgaggttgc
    10921 aacgccagcg cctgctgctg atcggccgcc tgcaacgctt gcagcgcctg ggcctagccg
    10981 acgaggtgca gcccggcacc tgggccattc atgccgatgc cgagaagacc ttgcgtgccc
    11041 tgggtgagcg tggcgacatc atccgaacga tgcagcgggc catgagcggc cagccgcgtg
    11101 agctggcggt gttcgagccg ggcgacgatg gtcgcagcct catcggccgc gtggccgcga
    11161 aggggcttgc cgatgagttg cacgaccgag gctatctgat catcgacggc acggacggta
    11221 aggcccacta cgttgcactg aacgcccgcg acgaactggc gaactatccc acgggcgctg
    11281 tggtagaggt gaagggttcg actgaggtgc gcacggccga caagaacatc gcctcgctgg
    11341 cgagcgatgg cctgtaccgc accgatcatc acctggtaat cgagcagggc cgggctacgc
    11401 ccggacgcga cccgcaggaa gtcgtcgccg ctcatgtccg gcggctggaa gccctgcgcc
    11461 gggcttgcat cgtggagcgc gtggccgaag ggctatggaa ggtaccggac gacctggccg
    11521 agcgtggccg ccaatacgac gcgcaacggc tgggcgatgt agccgtagag ctgaaatcac
    11581 accttcctat tgaacggcag gcccgcgtca tcggcgcgac ttggcttgac cagcaactga
    11641 tcggcggtgg ccgtgggctg ggcaacttgg gctttggcgg tgataccaag caggccatac
    11701 agcagcgagc cgatttcctc gaagaacagg ggctggcaca gcgccgcggg cagcgcgtca
    11761 tcctcgcgcg caacctgctg ggcacgctac gcaatcggga actagtgcag gccgcgaaag
    11821 acattgcggc ggaaaccggc ctagagcatc gcccggtggc cgacgggcag cgcgtagccg
    11881 gtatctaccg gcgttccatt atgctcgcca gcgggcgcta cgcgatgctc gatgacggca
    11941 tggggttcag ccttgtgccg tggcggccgg taattgagcc gcgactggga caacagcttg
    12001 ccgcaaaggt tatcggcgga agtgcctctt gggaacttgg acgaaagccc agtatcggta
    12061 tcagttagat atcaagggaa gctgagtacc aggcgcagtc tgcttggctg ctgtgattct
    12121 tcttagagat tcaatcaatg ccataaactc cttggattgc ctcagtgctt cgcctcccgc
    12181 agtttgcagg tccaagacat ccgtagcctc gatcgtgaag gctgtgggca cgcgcagcag
    12241 cgaatgccgg atcttgtcgt ccggcacgtc gttcagactt acgcttacta cgtagatttc
    12301 tgcgtcggcc gcaaaggggc tgtccgcttg gccacgggtg cggacaatct cggaacgcca
    12361 gtgttgcagg tcgtcgttaa gcatggccag cgtcacctgg gtctcgcgag cgccggcacc
    12421 gaagatcagc gactcaagta cttgcgtcgt gctgggaact cggtcgctac tgtccacgcg
    12481 ctcgctcagt ccgcgttcgg agttcaccgt caccaacacg agtcggcgca ggcttccggg
    12541 cttgacgtcc gagaagttgg cgctcattga gcccgaagct actagccggt ctagcatcag
    12601 acgtacgccg aggttgtcgg agacaccacc gtccacgagg tggatatagg gccgatcgtg
    12661 cgcattgagg tagctgtcta tgccgctttg caggatgcgt gatctgtaat tttgccctct
    12721 gacaggcaaa gatcggagag gcggcggaca ggtattggcg ttgtttcgca ccgttacggg
    12781 cgacagcacc agcggtactg ccgacgaggc cgccaccgcg aaagacagcg gcgttgcatc
    12841 gaggtcggaa cagattagcc ggaattgctc ggtggtgaaa tcgaaagggg caccggtcgt
    12901 gaggtcggtt gccgtgatca tcagttcggg cgcgcccggt cgtcggcgca cgtcaccaaa
    12961 ggtgcggcct tcaaaaagtt cgtccaagcg ttgagccagg atctggctgc gcccaaacca
    13021 aggtgaagtt agtctataaa gacgctcagg ccacagagct tcacgtatga gtcgcccctc
    13081 aaaaggcaca agcagaaaat ccggctcgaa acgagtcagc gtggcatcgc cgaaggccgc
    13141 gaagtgcgcg gcaaggatgc tgccccccga cactccgctg accaggaaca cctgatccaa
    13201 cagagtggtc tcctggccct ctagcgcgaa gcgtgtggcc ttgagttcgc gcagcacgcc
    13261 caggccgaag gccgccgccc gagcgccccc gcctgacagt gtcaccgcta caaccaccgg
    13321 ttggttgacc tccgacggcc tgatgcgttg gtcgttctcg gcgagagaca ttaaggcggc
    13381 gtcggaaagt gtcgggttga tccaaggacg gtaagtggaa caccctgtca gaactaccgt
    13441 tgccagcaca ccggccaaac tccatcgtgt gaatttacac acctccaggc gctttacctc
    13501 tgcaagcagc accacgcagc gtttcgaata gacaagaacg agaaacgccg gcaattttgc
    13561 gacgaagcag agccactcca gaatgctgca gacgttacaa gaacggcacc ctacgaatcc
    13621 ccttgatgag gctcctcgcg atccgcgcga agttatggaa tagcagctat tcacgagcca
    13681 gcacttctcc aagcgctatt gatccgttca aggcaggtgg tgattgcaga ctttgcatgc
    13741 ccgagcagtg tgtaggagcc tttgaaatcc gcgagagcgt tgccgtgctc gaagcagcta
    13801 gcagcagcca cccgacgatc tgtttgttca tctactgcct caaactccaa actagctccc
    13861 ccagttgtca ctggtccaac aagcaacgtc gttagggtgt tgactgctct attgggcgtc
    13921 tcaatggtcg taattgccac acgcacacgt atgcggcctg acgatgcggg agcttcggac
    13981 ttagtttgga gatgtcgcag ttcactgttg acgtgatcga gaagctctcg cacttgctca
    14041 gcttcaagcc cacctatgcg tccagttgcc gtcactacct gagcatcctc gactaatatt
    14101 tcccggtaac gactgggttc aaagcctggc gccttgaaga gcagaacatt ttcgtacttc
    14161 gtttttccca atacatcgta atcaccaaga aaccctgatc gagtcattcc gtttgaagca
    14221 cagccagata aggttgccgt gaggcctgcc agcagcacaa aatggattaa tgaaataggg
    14281 cgtttcatat atattttcat ttatcccatt ttcagacaga cgatgccagc aacaa
//
LOCUS       DAOMVO010000027        14283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_930_length_14283_cov_12.851560, whole genome shotgun sequence.
ACCESSION   DAOMVO010000027 DAOMVO010000000
VERSION     DAOMVO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_14283_cov_12.851560"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..644)
                     /gene="folK"
                     /locus_tag="PKV30_02740"
     CDS             complement(162..644)
                     /gene="folK"
                     /locus_tag="PKV30_02740"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRL29169.1"
                     /translation="MSAAAARAPVIAYVALGANLGDAAAVVASAIDALNRLADTRLEA
                     RSHLYRSAPVDAQGPDFINAVAAVSTRLTAPDLLLALQQLEQRAGRERPYRNAPRTLD
                     LDLLLYGDARIDSPSLTVPHPRMMERAFVLIPLAEIAPDLVPAAALAAVAGQRIERLS
                     "
     gene            complement(641..2236)
                     /gene="pcnB"
                     /locus_tag="PKV30_02745"
     CDS             complement(641..2236)
                     /gene="pcnB"
                     /locus_tag="PKV30_02745"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HRL29170.1"
                     /translation="MINKFIGRLIDKATAGSKGRKRFGKRAEIGPQGHAINPALVDER
                     AANVVRTLKEAGYEAYIVGGAVRDLLLGLKPKDFDVATNATPEQVKALFRRAFIIGRR
                     FRIVHVVYGRGREHEVIEVSTFRAYMDNATSNAMVQGNERSAKGQLAGMTHAVDASGR
                     VLRDNVWGSQEEDAARRDFSVNAMYYDPETRVVVDYHNGLQDSQKKLLRMIGDAATRY
                     REDPVRIMRAVRFGAKLAALGFTLEPKTAKPLVEARALLAEVPQSRLFDEMIKLLQTG
                     HAVPSVDQLRKLGLGKGIYPLLDVVVERADQPFVKAALADTDRRVAEGKPVAPSFLLA
                     CVLWQDVRDGWNARLAKGEHPFPALQDAIDEVFDARIGDVSGRGKLGADMREIWMMQP
                     RFEKRSGNTPFSLAEQPRFRAGFDFLRLRADVGEIDEELADWWQAFSLADDGERESLV
                     RELRAEQQRSKKQQPPRTHRVPKQAPTQEGQAPAAIESEASGEGDEASPTPAKKRRRR
                     RRKPSGGGGAEGQGGGVEPSGDA"
     gene            complement(2233..2913)
                     /locus_tag="PKV30_02750"
     CDS             complement(2233..2913)
                     /locus_tag="PKV30_02750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL29171.1"
                     /translation="MKLALFDLDHTLIPIDSDHAWGEFTTRIGWTDAAEFKRRNDAFY
                     EQYKAGTLDIAEYIRFTTAAIRRHGPGASAEAQARFMREVIAPAIKPQARALVEAHRK
                     AGDALVIVTATNEFVTRPIAHSFGIDELIAVDLARGPGGWITGEIAGVPSFREGKVTR
                     VAQWLAARGRSFADVDITFYSDSRNDLPLLEKSQNPVATNPDDTLRAIATERGWRILE
                     LFPQEENA"
     gene            complement(2956..3642)
                     /gene="hda"
                     /locus_tag="PKV30_02755"
     CDS             complement(2956..3642)
                     /gene="hda"
                     /locus_tag="PKV30_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HRL29172.1"
                     /translation="MKQIALDIGLAPEPTLDGFVAGRNAAALQHLRLWSESAARSPVP
                     TYLWGPEGAGKTHLLRAARAQLQAQRVAVGWLDADTPPRTPFNEQWGAVLMDDVHLYD
                     TELQHTAFNWFVNAVAPANGRARPVLAAGLLPPADLPLREDLRTRLGWGHVFELHVLP
                     ESDMRAVLRRAADARGVFLSDEVMNFVLGRFSRDLGSLMQLLDHLDCYALQTQRAITI
                     PLIKSMLQNE"
     gene            3781..4818
                     /gene="purM"
                     /locus_tag="PKV30_02760"
     CDS             3781..4818
                     /gene="purM"
                     /locus_tag="PKV30_02760"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRL29173.1"
                     /translation="MTASPTPLSYKDAGVDIDAGDALVERIKPLAKKTMREGVLSGLG
                     GFGALFEVPKRYQEPVLVSGTDGVGTKLRLAFEWDLHDTVGVDLVAMSVNDVLVQGAE
                     PLFFLDYFACGKLDVDTATSVVGGIAKGCELAGCALIGGETAEMPGMYPPGEYDLAGF
                     CVGAVEKSKILTGLNVAEGDVVLGLASSGVHSNGFSLVRKCIERAGDDLPATLDGQPF
                     KQAVMAPTRIYVKNVLAALEKGTIKALAHITGGGLLENIPRVLPDGLAAHLTKGSWPQ
                     SELFAWLQKTAGIDDIEMNRTFNNGIGMVVVVDAASAAACAATLRAAGEQVYDIGRIA
                     PRGTGAAVVVA"
     gene            4903..5865
                     /locus_tag="PKV30_02765"
     CDS             4903..5865
                     /locus_tag="PKV30_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRL29174.1"
                     /translation="MAASLLLIMWQGLLPGLLCVCLGFLATRWFSPRLGALTRAHDHQ
                     APKLAAGLVALIPVVLIAVFLPRTRGVILDAPNTYRELLAFMARTVLELRDRLPPDIA
                     AQLPTGAEDVQRVIAAYLATKAGTLATTGRAWLSGLLFAYIGLLVGALAAARPAVIML
                     KPLAAQLHLRLTRFGETFRQIVVAQFWIALFNTFLTAIFLLVVLPLWDSRLPYTMALI
                     LLTFLAGLIPIVGNLICNGVLTLVGLSVSPLVALACLVFLIAIHKAEYFINAKVIGHR
                     THMGVWELLAVMFVMEAVFGPAGLVAAPLFYAYTKKELQASGWV"
     gene            complement(5964..6815)
                     /locus_tag="PKV30_02770"
     CDS             complement(5964..6815)
                     /locus_tag="PKV30_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRL29175.1"
                     /translation="MQFDFSAPTPLAYFATLVQRDDGLPLLEAAASLGQDDHPALDVQ
                     QVLHDVDQLAARLQRRVKPDADAMERLRALNQFFFGDLGFAGNVNDYYNPDNSFLHAV
                     LHTRRGIPISLAVLWLELAQGLGLQAEGVGFPGHFLVQVNLNEGRVVIDPFTGQSLGR
                     SELRERIEEIHPDLAAAPGGDLPLALYLRPAGPRQILARMLRNLQDIHRTQEDWRRFV
                     QVQDRLITLLPDAWPEYRDRGLALAELGEAGRAHADLALYLREARQANDLERVAERLD
                     ALRRSLP"
     gene            complement(6871..8424)
                     /gene="murJ"
                     /locus_tag="PKV30_02775"
     CDS             complement(6871..8424)
                     /gene="murJ"
                     /locus_tag="PKV30_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRL29176.1"
                     /translation="MSLFKSASTVSLLTLVSRITGLVRDLFMASAFGVSGLTDAFNVA
                     FRIPNLLRRLFAEGAFSQAFVPVLARSRAANGEDATKKLIDAVATALAWALVATCIVG
                     VAGAPVLVWLLASGLDAHSFDAAVLMTRWMFPYIACMSLVALSAGILNTWRHFAVPAA
                     TPVLLNLAMIVAAWLGAPWFGRLGLEPIYAMAAGVMAGGVLQLAVQVPALKRLGLLPR
                     IGLGWARVRDAWRDAGVRQVLGLMLPALLGVGVAQLSLLINTQIASHLAAGSVTWVFY
                     ADRLMEFPTALLGVALGVVLTPQLAAARASNDDARYSQMLDWGLRLVLLLALPCAAAL
                     LVFATPLIATLFHHGKFSAGDVHASALALSLYGVGLLGLVAVKVLAPGYYASLDIRTP
                     VKIAVAVLVITQLFNLIFVPLLQHAALTLSIALGAMINALWLLIGLIRRGSYRPQGGW
                     AVFGAQVLGATLLMSAFLLWAARRFDWLGTAAWPRAGLLALVLMAAAALYFGALMASG
                     VRLRQLLRR"
     gene            8564..9715
                     /locus_tag="PKV30_02780"
     CDS             8564..9715
                     /locus_tag="PKV30_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL29177.1"
                     /translation="MPAARLALVARLSARVSALVALPVLVLAACASAPFPYADPALPH
                     RGPDGFRNIGSDARPGGSAPWYEVLQRRWRGDFKPSGPPEGGYAAFAERWRQPLKPST
                     LEVAAASPTPQLAWLGHAGLWLQAGGQRVLIDPQLSSFAGPVSWLASKRLIDAPIAAA
                     DLPPVDLLLITHSHYDHLDIATLKPILARHPQPRVLVPLGLKKWMQDQGARQVEELDW
                     WEQRQVGPLTVRLLPAQHWSRRGLSDTNQTLWGGWDIAWPRPGGGAPWRLVHTGDTGY
                     NEALYRQIGERLGGPVDLVAVPIGAYEPREFMRAQHNNPDEAVRIARLLNARRALGIH
                     WGTFEISHEPLDQPPRDVAAALKAQALPPDHVWLMKQGEVRALPVEEAR"
     gene            9934..10239
                     /gene="rpsT"
                     /locus_tag="PKV30_02785"
     CDS             9934..10239
                     /gene="rpsT"
                     /locus_tag="PKV30_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRL29178.1"
                     /translation="MAAGKPKKKNPRLASGRKRARQNVKLNAANTSLRSKYRTAVKNV
                     EKAVLAGDKEKASSAFALMQSVVDTVADKGIFHKNKAARDKSRLSAKVKTLATQAAA"
     gene            complement(10540..10902)
                     /locus_tag="PKV30_02790"
     CDS             complement(10540..10902)
                     /locus_tag="PKV30_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HRL29179.1"
                     /translation="MAATPSSNEVFIRGITRDGRTFRPSDWAERLAGVMAQFRPGGAG
                     GLGGHIAYSPWCVPTTLDGAKCVVVNRALRDHEPMAWDFVMGFARDNELQVIEACLLP
                     DASPSESGPNPMTGAGAP"
     gene            11098..12294
                     /locus_tag="PKV30_02795"
     CDS             11098..12294
                     /locus_tag="PKV30_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL29180.1"
                     /translation="MTAHIEAASPHTMNTYARLPIAMDHGQGCYVWDVNGKRYLDGLG
                     GIAVNTLGHNHPKLVPALQDQIGKIIHSSNYYHVPNAEVLAKKLTELSGLTNVFFCCT
                     GLEANEAALKLARKFGHDKGIANPQIVVYEKAFHGRSIATLSATGNPKIQKGFEPLVE
                     GFIRVPLNDIEALKKATEGNPNVVAVFFETIQGEGGINPMRADYLKQVRALCDEKDWL
                     MMIDEVQCGMGRTGKWFAHQWAGIKPDVMPLAKGLGSGVPIGAVVAGPKAANIFQPGN
                     HGTTFGGNPLAMRAGVETIRIMEEEKLLDNAAKVGAHLKAALEKALAGNPGVKDIRGQ
                     GLMLGIELAKPCGVILQRAADAGLLLSVTADTVIRLVPPLILSEHEADEIVAILVPLI
                     EAFLKE"
     gene            12297..13229
                     /gene="argF"
                     /locus_tag="PKV30_02800"
     CDS             12297..13229
                     /gene="argF"
                     /locus_tag="PKV30_02800"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRL29181.1"
                     /translation="MAEPIKHYLQFADFTAAEYAYLFERAALIKGKFKAYEKHHPLSD
                     RTLAMIFEKASTRTRVSFEAGMYQLGGSVVNLTSGDSQLGRAEPIEDTARVISRMVDI
                     VMIRTFEQARIEQFAAHSRVPVINGLTNEYHPCQILADIFTFIEHRGPIQGKTVAWIG
                     DGNNMANTWAQAAALLGFTLRVSTPRGYEMDTQLAALDGTPLTKGWQNFNDPMKACEG
                     ADLVTTDVWTSMGYEAENEARRKAFADWCVDGDMMAAAKADALFMHCLPAHRGEEVTA
                     EVIDGPQSVVWDEAENRMHVQKALMEYLLLGRQS"
     gene            complement(13344..13961)
                     /locus_tag="PKV30_02805"
     CDS             complement(13344..13961)
                     /locus_tag="PKV30_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29182.1"
                     /translation="MSEEQDDPYVMLTPAGALHAYADRAPDETCAALQTLMPRGASLR
                     RSAWLAQMASHTILLKRALHEGWVHEVGRELQAPDVRLDNYLPHAIAGLSGSRMAALA
                     SEDGFCLARVGYSQDEADTLCVAAADFFDFVARQKQRGFKGTGRAVSFHEGIDMLMPT
                     TTFMLFWVDGTGYWLIVGGEPLLNNRALVELIWGIRVAGTKFAVQ"
     gene            complement(13958..>14283)
                     /locus_tag="PKV30_02810"
     CDS             complement(13958..>14283)
                     /locus_tag="PKV30_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL29183.1"
                     /translation="DFMWDILTENALGLVLMLKGNAEDPVSDLRTYVTEFRDFIDKTS
                     LVVGITHTDTGGWQTRQRVSSELLAMGLPPTAMDTDARSRAHMATLVKALIYGIDPFN
                     GSAKP"
BASE COUNT         2462 a         4841 c         4779 g         2201 t
ORIGIN      
        1 acgcaaacgt catgaagacg ttgctggcca tcagcagcag cacggtctgc cacgaaacgg
       61 gcagcgattg cagggtttgc atcatgggct agggttacta ctgttttgat agcttcttgc
      121 gcttgatggg caagcgctgg cgctgttttt cattcacaac ctcagctcaa ccgctcaatc
      181 cgctgccccg ccaccgccgc cagggcagca gccggcacga gatcgggcgc aatctcggcc
      241 agcggtatca gcacgaaggc gcgctccatc atgcgcgggt gcggcacggt cagcgacggg
      301 ctgtcgatgc gcgcgtcgcc gtagagcagc agatcaagat cgagcgtgcg cggcgcgttt
      361 cgatagggac gctcgcggcc tgcgcgctgc tccagttgtt gaagcgcgag cagcaaatcc
      421 ggcgcggtca ggcgggtgga aacggcagcc acagcgttga tgaaatcagg gccttgtgca
      481 tcgactggcg cgctgcggta cagatgcgag cgcgcctcca gccgtgtgtc cgccagtcga
      541 ttcagcgcat cgatggccga ggccacgacg gcggcggcgt cgcccaagtt ggcgccgagc
      601 gcgacatagg cgatcaccgg cgcacgcgcc gcagcggcac tcacgcgtcg ccggaaggtt
      661 ccacgccgcc gccctgcccc tcagcgccac cgccgcccga cggcttgcgc cggcgccgcc
      721 ggcgcttttt ggccggcgta ggcgaggcct cgtcgccctc gccagacgct tcagattcaa
      781 tagctgctgg cgcttgtccc tcctgcgtcg gcgcttgttt tggcacccgg tgcgtgcgcg
      841 gtggctgctg cttcttgctg cgttgctgct cggcacgcag ctcgcgcacc aggctttcgc
      901 gctcgccgtc gtcggccagg ctgaaggcct gccaccagtc ggccagttct tcatcgatct
      961 cgcccacatc ggcacgcagg cgcagaaagt cgaagccggc gcgaaagcgc ggctgctcgg
     1021 ccaggctgaa gggggtgttg ccgctgcgct tttcaaagcg cggctgcatc atccagatct
     1081 cgcgcatgtc ggcgcccagc ttgccgcggc ccgacacgtc gccgatgcgg gcgtcaaaca
     1141 cttcgtcgat ggcgtcctgc agggcgggga acgggtgctc gcccttggca agccgcgcgt
     1201 tccagccatc gcgcacgtcc tgccacagca cgcaggccaa gaggaatgac ggcgccaccg
     1261 gcttgccttc ggccacgcgg cgatcggtgt cggccagcgc cgccttcaca aagggctggt
     1321 cggcgcgctc aaccaccaca tcgagcagcg ggtaaatgcc cttgcccagg cccagcttgc
     1381 gcagttgatc gacgctgggc acggcgtggc cggtttgcag cagcttgatc atttcgtcga
     1441 acaagcggct ttgcggcacc tcggccagca aggcgcgcgc ttcgaccagc ggcttggctg
     1501 tcttgggctc gagcgtgaag ccgagcgccg ccagcttggc gccaaagcgc accgcacgca
     1561 tgatgcgcac cgggtcttcg cggtagcgcg tggcggcatc gccgatcatg cgcagcagct
     1621 ttttttgcga atcctgcagg ccgttgtggt agtcgaccac cacgcgcgtc tcggggtcgt
     1681 aatacatggc gttgacgctg aagtcgcgcc gcgcggcgtc ttcttcttgc gagccccaga
     1741 cgttgtcgcg cagcacgcgg cccgacgcat caacggcgtg cgtcatgccg gccagctggc
     1801 ctttggccga gcgctcgttg ccctggacca tcgcattgga ggtggcgttg tccatgtaag
     1861 cgcggaaggt ggagacctcg atcacctcgt gctcgcgccc acgcccgtac accacgtgca
     1921 cgatgcgaaa gcgccggccg atgatgaagg cgcggcggaa cagcgccttg acctgttcgg
     1981 gcgtggcgtt ggtggcgacg tcgaagtcct tcggtttcag gccgagcagc agatcgcgca
     2041 cggcgccgcc gacgatgtag gcctcgtagc ccgcctcttt cagcgtgcgc accacgttgg
     2101 cggcgcgttc atcgaccagc gcggggttga tcgcatggcc ttgcgggccg atctcggccc
     2161 gcttgccgaa gcgcttgcgg cccttgctgc ccgccgtggc cttgtcgatc agccggccga
     2221 tgaacttgtt gatcatgcgt tttcttcttg gggaaacaat tcaaggatgc gccagccgcg
     2281 ctcggtggcg atggcgcgca gcgtgtcgtc ggggttggtg gccacgggat tttgcgactt
     2341 ttcaagcagc ggcaggtcgt tgcgcgaatc tgaataaaag gtgatgtcca cgtcggcaaa
     2401 cgagcggccg cgcgcggcca gccattgggc cacacgcgtg accttgcctt cgcggaacga
     2461 gggcacgccg gcgatctcgc ccgtgatcca gccgccgggg ccgcgcgcca ggtcaacggc
     2521 gatcagctcg tcgatgccga agctgtgcgc aatgggccgc gtgacgaatt cgttggtcgc
     2581 agtcacgatg accagcgcgt cgcccgcctt gcggtgcgct tcgaccagcg cgcgcgcctg
     2641 cggcttgatg gccggcgcga tcacctcgcg catgaagcgc gcctgcgcct cggccgacgc
     2701 gcccgggcca tggcggcgga tggcggcggt ggtgaagcgg atgtattcgg caatgtcgag
     2761 cgtgccggcc ttgtactgct cgtagaaggc gtcgttgcgg cgcttgaact cggcggcatc
     2821 cgtccagccg atgcgggtgg tgaactcgcc ccaggcgtgg tccgagtcga tggggatcag
     2881 ggtgtggtcg aggtcgaaga gggccagttt cataatcaaa aagggttcca gcgcaagcgg
     2941 gacaagcgcg agcagctatt cattttgcag catcgacttg atgagcggaa tggtgatggc
     3001 gcgttgcgtc tgcagcgcgt agcagtccag atgatccagc agctgcatca ggctgcccag
     3061 gtcgcgcgag aagcggccga gcacgaagtt catcacctcg tccgacagaa aaacgccgcg
     3121 cgcgtcggcg gcgcggcgca gcacggcgcg catgtcggat tcgggcagca cgtgcagctc
     3181 gaacacgtgg ccccagccga ggcgtgtgcg caaatcttcg cgcaacggca gatcggccgg
     3241 cggcagcaag ccagccgcca gcaccgggcg cgcgcggccg ttggcgggcg ccacggcgtt
     3301 gacaaaccag ttgaaggcgg tgtgctgcag ctcggtgtcg tacagatgca cgtcgtccat
     3361 cagcacggcg ccccactgct cgttgaaagg cgtgcgcggc ggtgtgtcgg cgtcgagcca
     3421 gccgacggcg acgcgctgcg cctgcagttg cgcgcgtgcg gcgcgcagca ggtgggtctt
     3481 gccggcgcct tcggggcccc acaaataagt cggcacgggc gagcgagcgg cgctttcgct
     3541 ccacaaacgc aggtgctgca gcgcggcggc gttgcgcccg gcgacgaagc cgtccagcgt
     3601 cggctcgggc gccaggccaa tatcgagcgc gatctgtttc atggggcgcg cgacacgcgg
     3661 cgcgctggca aaaagaagag catgaggggc gggttcgacg cgggccgctg cggccagaga
     3721 ggcatcggcc agcgcctaga atcaaggctt gattttacgt ggcacccacg cctcatctgc
     3781 atgaccgctt cccccacccc cttgagctac aaagacgccg gtgtcgacat cgacgcgggc
     3841 gacgcgctgg tcgagcgcat caagccgctg gccaaaaaga cgatgcgcga gggcgtgctg
     3901 tcgggcctgg gcggttttgg tgcgctgttc gaggtgccca agcgctacca ggagccggtg
     3961 ctggtcagcg gcaccgacgg cgtgggcacc aagctgcggc tggcttttga gtgggacttg
     4021 cacgataccg tgggcgtcga cctggtcgcc atgagcgtca acgacgtgct ggtgcagggt
     4081 gccgagccgc tgttcttcct cgactacttc gcctgcggca agctggatgt ggacacggcc
     4141 accagcgtgg tcggcggtat cgccaagggc tgcgagctgg ccggctgcgc gctgatcggc
     4201 ggcgaaactg ccgagatgcc cggcatgtat ccgccgggcg aatacgacct ggccggcttt
     4261 tgcgtcggcg ccgtcgaaaa gtccaagatc ctgaccgggc taaacgtggc cgaaggcgac
     4321 gtggtgctgg ggctggcgtc aagtggcgtg cacagcaacg gcttcagcct ggtgcgcaaa
     4381 tgcatcgagc gcgcgggcga cgatttgccc gccacgctgg acggccagcc gttcaagcaa
     4441 gcggtgatgg cgcccacgcg catctacgtg aaaaacgtgc tggccgcgct tgaaaaaggc
     4501 acgatcaagg ccctcgccca catcaccggc ggcggcctgc tcgagaacat cccgcgcgtg
     4561 ctgcccgacg gcctggccgc gcacctcacc aaaggcagtt ggccgcaaag cgagctgttc
     4621 gcctggctgc aaaaaaccgc cggcatcgac gacatcgaga tgaaccgcac cttcaacaac
     4681 ggcatcggca tggtggtcgt ggtcgatgcc gccagcgcgg ccgcctgcgc cgccaccctg
     4741 cgcgcggccg gcgaacaggt gtacgacatc ggccgcatcg ccccgcgcgg cacgggcgcg
     4801 gcggtggtgg tggcctgacc gagccggacg gccgagcccc ccttggtcac cccgcaatac
     4861 gagcgccgcg aacgcatcgc caccgtggcc agccacgtgg cgatggcggc ctcgctcctg
     4921 ctcatcatgt ggcaggggct gctgcccggt ttgctgtgcg tgtgcctggg ctttctggcc
     4981 acgcgctggt tcagcccgcg gctgggggcg ctgacgcgtg cgcacgacca ccaggcgccc
     5041 aagctggccg ccgggctggt ggcgctgatt ccggtggtgc tgatcgccgt gtttctgccc
     5101 cgcacgcgcg gcgtcatcct ggacgcgccc aacacctacc gcgagctgct ggccttcatg
     5161 gcgcgcacgg tgctggagtt gcgcgaccgc ctgccgcccg acatcgcggc ccagttgccc
     5221 accggcgccg aagacgtgca gcgcgtcatc gccgcctacc tggccaccaa ggcgggcacg
     5281 ctggcgacca ccgggcgggc ctggctgtcg ggcctgctgt ttgcctacat cggcctgctg
     5341 gtcggcgcgc tggctgcggc gcgccccgcg gtcatcatgc tcaagccgct ggcggcacaa
     5401 ctgcacctgc ggctgacgcg ctttggcgag acctttcgcc agatcgtggt ggcgcagttc
     5461 tggatcgcgc tgttcaacac ctttctcacc gccatcttcc tgctggtggt gctgccgctg
     5521 tgggacagcc gcctgcccta caccatggcg ctgatcctgc tgacctttct ggccgggctg
     5581 atccccatcg tcggcaacct gatctgcaac ggcgtgctga cgctggtggg cctgtcggtc
     5641 tcgccgctgg tggcgctggc ctgcctggtg tttctgatcg ccatccacaa ggccgaatac
     5701 ttcatcaacg ccaaggtcat cggccaccgc acgcacatgg gcgtgtggga gctgctggcc
     5761 gtgatgttcg tgatggaagc cgtgttcggc ccggccgggc tggtggcggc gccgctgttt
     5821 tacgcctaca ccaagaaaga actgcaggcg tcgggctggg tctaggcgcg ggcctgaaca
     5881 cgccgcgaat actacggtat tgatagcttc ttgcgctttt ccatcaggcg ctggcggcct
     5941 aaaacacttg aaaactcagg cgttcatggc agcgaacgcc gcagcgcgtc cagccgttcg
     6001 gccacgcgct caaggtcgtt cgcctggcgc gcctcgcgca ggtacagcgc gaggtcggcg
     6061 tgcgcacgcc cggcttcgcc cagctcggcc aacgccagcc cgcgatcccg gtattcgggc
     6121 caggcgtcgg gcagcagcgt gatcaggcgg tcctgcacct ggacgaagcg gcgccagtcc
     6181 tcttgcgtgc ggtggatgtc ctgcaaattg cgcagcatgc gggccaggat ctggcgcggg
     6241 ccggccgggc gcaggtacag cgccagcggc aggtcgccgc ccggcgcggc agccaggtcg
     6301 gggtgaattt cctcgatgcg ctcgcgcagc tcgctgcggc cgagcgactg gccggtgaag
     6361 gggtcgatca ccacccgccc ctcgttcagg ttgacctgca ccagaaaatg ccccggaaaa
     6421 cccacgccct cggcctgcag ccccagcccc tgggccagct ccagccacag cacggccagc
     6481 gagatgggaa tgccgcggcg cgtgtgcagc accgcgtgca aaaagctatt gtcggggttg
     6541 tagtagtcgt tgacgttgcc ggcaaagccc aggtcgccaa agaagaactg gttgagcgcg
     6601 cgcaggcgct ccatggcgtc ggcatcgggc ttcacgcggc gctgcaggcg ggcggccagc
     6661 tggtcgacat catgaagcac ctgctgcacg tcgagcgcgg ggtggtcgtc ctgccccagg
     6721 ctggcggcgg cctccagcag cggcaggccg tcgtcgcgct gcaccagcgt ggcgaagtag
     6781 gcgagcggcg tgggggcgga aaaatcaaac tgcatgaccg ctttgtagcg gcagcgcgcg
     6841 ggccgcgtca atgccggctt gccagcgctt tcagcggcgc agcaactgcc gaaggcgcac
     6901 gcccgaggcc atcagcgcgc caaagtaaag cgccgccgcc gccatgagca cgagcgccag
     6961 cagtccggcg cgcggccagg cggcggtgcc caaccagtcg aaacggcgcg cggcccacag
     7021 cagaaacgcg ctcatcagca gcgtggcacc cagcacctgg gcgccaaaca ccgcccagcc
     7081 gccctgcggc cggtagctgc cgcggcgaat caatccaatc agcagccaca gcgcgttgat
     7141 catggcgccc agcgcaatcg acagcgtgag cgccgcgtgc tgcagcagcg gcacgaagat
     7201 caggttgaac aactgcgtga tgaccagcac cgccaccgcg atcttgaccg gcgtgcgaat
     7261 gtccaggctg gcgtagtagc ccggcgccag caccttgacg gccaccagcc cgagcaggcc
     7321 cacgccgtac agcgacagcg ccagcgctga agcgtgcacg tcgcccgccg agaacttgcc
     7381 atgatgaaac agtgtggcga tcagcggcgt ggcaaacacc agcagcgccg ccgcgcaggg
     7441 cagcgccagc agcagcacca ggcgcaggcc ccagtcgagc atctgcgaat agcgcgcgtc
     7501 gtcattgctg gcacgcgccg ccgccagttg cggcgtcagc acgacgccca gcgccacgcc
     7561 gagcagcgcg gtggggaact ccatcagccg gtcggcgtaa aacacccagg tcacgctgcc
     7621 ggccgccagg tgcgaggcga tctgcgtgtt gatgagcagc gacaactgcg ccacgcccac
     7681 gccgagcagc gccggcagca tcaggcccag cacctggcgc acgcccgcgt cgcgccaggc
     7741 atcgcgcacg cgcgcccagc cgagcccaat gcgcggcagc agccccaggc gcttgagcgc
     7801 cggcacctgc accgccagtt gcagcacgcc gccggccatc acgccggccg ccatggcgta
     7861 gatcggctcc agccccaagc gcccgaacca cggcgcgccc agccaggcgg cgacgatcat
     7921 cgccaggtta agcagcaccg gcgtggccgc cggcacggca aaatgccgcc acgtgttgag
     7981 aatgccggcc gacagcgcca ccagcgacat gcaggcgatg taggggaaca tccagcgcgt
     8041 catcagcacg gcggcgtcga agctgtgcgc gtccagcccg ctggccagca gccacaccag
     8101 caccggcgcg ccggccacgc cgacgatgca ggtcgccacc agcgcccagg ccagggcggt
     8161 ggccacggcg tcgatcaatt tcttggtggc gtcttcaccg ttcgccgccc gcgagcgcgc
     8221 cagcaccggc acaaacgcct ggctgaacgc gccttcggca aacaggcggc gcagcaggtt
     8281 gggaatgcga aaggcgacgt tgaaagcgtc cgtcaggccg ctcacgccaa aggccgaggc
     8341 catgaacagg tcgcgcacga gcccggtgat gcgagacacc agggtgagca gcgagacggt
     8401 ggaggcggac ttgaaaagcg acacggggcg atgcgtcgga cgcgcacggc acgtcgaacg
     8461 ggcgccagtg taggcgagcg gctggcggcg ccggtgccgc cgccggccgc gccgagaaaa
     8521 acccgcgcgt gcaagcccca tgggctgcaa ggcatgatcg cgcatgcccg ccgcccgcct
     8581 cgcccttgtt gcgcgccttt ctgcgcgcgt ttctgccctg gtcgccctgc cagtgctggt
     8641 gctggccgcc tgcgccagcg caccctttcc ctacgccgac ccggcgctgc cgcaccgcgg
     8701 gccagatggc tttcgcaaca tcggcagcga cgcgcgcccc ggcggcagcg cgccctggta
     8761 cgaggtgttg cagcggcgct ggcgcggcga cttcaagcca tctggcccgc ccgagggcgg
     8821 gtacgcggcc ttcgccgagc gctggcgcca gccgctgaag cccagcacgc tcgaagtcgc
     8881 ggccgccagc cccacgccgc aactcgcctg gctcggccac gccgggctgt ggctgcaggc
     8941 gggcgggcag cgcgtgctga tcgacccgca actctcaagc tttgccgggc cggtgtcgtg
     9001 gctggcgtca aagcgcctga tcgacgcgcc gatcgccgcg gccgatttgc cgccggtgga
     9061 cctgctgctc atcacgcaca gccactacga ccatctggac atcgccacct tgaagcccat
     9121 cctcgcgcgc catccgcagc cgcgcgtgct ggtgccgctg ggcctgaaaa agtggatgca
     9181 agaccagggc gcgcgtcagg tcgaagaact cgactggtgg gagcagcgcc aggtcggccc
     9241 gctcacagtg cgcctgctgc cggcccagca ctggagccgc cggggtttga gcgacaccaa
     9301 ccagacgctg tggggcggct gggacatcgc ctggccccgc cccggcggcg gcgcgccctg
     9361 gcgcctggtg cacaccggcg acacgggcta caacgaggcg ctgtaccgcc agatcggcga
     9421 gcgcctgggc ggcccggtgg acctggtggc cgtgcccatc ggcgcctacg agccgcgcga
     9481 attcatgcgc gcgcagcaca acaaccccga cgaggcggtg cgcatcgcgc gcctgctgaa
     9541 cgcccgccgg gcgctgggca tccactgggg caccttcgag atcagccacg agccgctcga
     9601 ccagccgccg cgcgacgtgg ctgcggccct gaaggcgcag gcgctgccgc ccgatcatgt
     9661 gtggctgatg aagcagggtg aggtgcgcgc cctgccggtg gaagaggcgc gatgacggcg
     9721 ctgcgcgcca tgacctcggc tacgccgttg atgacgcgcc ctgcggggcg catgacgaat
     9781 tcatggcgcc cgaggcgccg tcattgagag cgaagccgaa gtcatgcgct cgtcagagcg
     9841 cggtcattcg tcgtcgttgg catgacaccc gcttcaaggg tataatgttg ggttttgccg
     9901 acaacatcca caaaccctta aaggaacact tccatggccg ctggtaagcc caaaaagaag
     9961 aacccccgcc tggcatcggg ccgcaaacgc gcccgccaga acgtcaaact gaacgctgcg
    10021 aacacctcgc tgcgctccaa ataccgcacc gcggtgaaga atgtcgagaa ggcagtcctc
    10081 gccggtgaca aggaaaaagc cagcagcgcc tttgcgctga tgcagtcggt ggtcgacacg
    10141 gtcgccgaca agggcatctt ccacaagaac aaggccgctc gcgacaagag ccgcctgtcg
    10201 gccaaggtca agaccctggc cacccaagcc gccgcctgac ggatcaattc aggcaaacag
    10261 cccggatggg cttcaagccc atccgccgtt tgtatcgggt gcgctgtcgg caaaaaagca
    10321 gagaaaccgc cttcgggcgg tttttttgtg ggcgtttact actgaaaacg tagctgctcg
    10381 cgcttaccat tcaagcgctg gcggcctttt ttaagaccga tgcaaatgac tgcgcctgcg
    10441 gcgcaatgac ttcggcttcg ccttcgatga cggcgcctct ggcgccatga cttcaatcat
    10501 gcgcccgcag ggcgcgtcat tgacggtcgc cggccgaggt catggcgcgc cagcgccggt
    10561 catcggattc ggcccgctct ccgatggcga tgcgtccggc agcaaacacg cctcgatcac
    10621 ctgcagctcg ttgtccctgg caaagcccat cacgaagtcc cacgccatcg gctcgtggtc
    10681 gcgcaacgcg cggttgacca ccacgcactt ggcgccgtcc agcgtggttg gcacgcacca
    10741 gggtgaatac gcgatgtggc cgcccaagcc gcccgcgccg ccgggccgga actgcgccat
    10801 caccccggcc agccgctcgg cccagtcgct gggccgaaag gtgcgcccat cgcgcgtgat
    10861 gccgcggatg aagacttcgt tgctggaggg ggtggcggcc atgaatgaag aggacggccg
    10921 gcacagttgg tgcgtgctgc ggcgcagcag aaaattgtag cgcctgccct gcgccgccac
    10981 gcccgcacgg cgcatgacag tcatgcaaac acgtcaacaa agcgccttgg cgcacaccct
    11041 agaatgcacc ttcagcgggc ctcgttggcc cgattttcat ttccaaggag ccttgtcgtg
    11101 accgcccata tcgaagccgc ctcgccccac accatgaaca cctacgcccg gctgccgatc
    11161 gccatggatc acggccaggg ctgctacgtg tgggacgtca acggcaagcg ctacctggac
    11221 ggcttgggcg gcatcgccgt caatacgctg ggccacaacc accccaaatt ggtgcccgcg
    11281 ctgcaggatc agatcggcaa gatcatccac tccagcaact actaccacgt gcccaacgcc
    11341 gaggtgctgg cgaaaaagct gaccgagctg tcgggcctga ccaacgtgtt tttctgctgc
    11401 accgggctgg aggcgaatga ggcggccctg aagctggctc gcaagttcgg ccacgacaag
    11461 ggcatcgcca acccgcagat cgtggtctat gaaaaagcct tccacggccg cagcatcgcc
    11521 acgctgagcg ccaccggcaa ccccaagatc cagaaaggct tcgagccgct ggtcgaaggc
    11581 ttcatccgcg tgccgctgaa cgacatcgag gcgctgaaaa aagccaccga gggcaacccc
    11641 aacgtggtgg ccgtgttctt cgaaaccatc cagggcgaag gcggcatcaa cccgatgcgc
    11701 gccgactact tgaagcaggt gcgcgcgctg tgcgacgaga aggactggct gatgatgatc
    11761 gacgaggtgc agtgcggcat gggccgcacc ggcaagtggt ttgcgcacca gtgggcgggc
    11821 atcaagcccg acgtgatgcc gctggccaag ggcctgggct cgggcgtgcc gattggcgcc
    11881 gtcgtcgccg gccccaaggc ggccaacatc ttccagccgg gcaaccatgg caccaccttt
    11941 ggtggcaacc cgctggccat gcgcgcgggg gtggaaacca tccgcatcat ggaagaagaa
    12001 aaactgctgg acaacgccgc caaggtcggc gcgcacctga aggccgcgct ggaaaaagcg
    12061 ctggccggca acccgggcgt gaaggacatc cgcggccagg gcttgatgct gggcatcgaa
    12121 ctcgccaagc cctgcggcgt gatcctgcag cgcgcggccg acgctgggct gctgctgagc
    12181 gtgaccgccg acaccgtgat ccgcctggtg ccgccgctga tcctgagcga gcacgaagcc
    12241 gacgagatcg tcgccattct ggtgccgctg atcgaggcgt ttctgaaaga gtgacgatgg
    12301 ccgagccaat caagcactac ctgcagtttg ccgacttcac ggccgctgaa tatgcctacc
    12361 tgttcgagcg cgccgcgctg atcaagggca agttcaaggc gtatgagaag caccatccct
    12421 tgagcgaccg cacgctggcc atgatcttcg agaaggccag cacgcgcacg cgcgtgagct
    12481 tcgaggccgg catgtaccag ctgggcggca gcgtggtcaa cctgaccagt ggcgacagcc
    12541 agttgggccg cgccgagccg atcgaagaca cggcgcgcgt catcagccgc atggtagaca
    12601 tcgtgatgat ccgcaccttc gagcaggcgc gcatcgagca gtttgccgcg cattcgcgcg
    12661 tgccggtgat caacggcctg accaacgaat accacccctg ccagatcctg gccgacatct
    12721 tcacgttcat cgaacaccgc gggccgattc agggcaagac ggtggcctgg atcggtgacg
    12781 gcaacaacat ggccaacacc tgggcacagg ccgccgccct gctcggcttc acgctgcgcg
    12841 tgtcgacgcc gcgcggttac gagatggaca cgcaactggc cgcgctcgac ggcaccccgc
    12901 tgaccaaggg ctggcagaac ttcaacgacc ccatgaaggc ctgcgaaggc gccgatctgg
    12961 tgaccaccga cgtctggacc tcgatgggct acgaggccga gaacgaggcg cgcaggaagg
    13021 cgtttgcaga ttggtgcgta gacggcgaca tgatggctgc cgccaaggcc gacgcgctgt
    13081 tcatgcactg cctgcccgcg caccggggcg aggaagtgac ggccgaggtg atcgacggcc
    13141 cgcagtccgt ggtgtgggac gaggcagaaa atcggatgca tgtgcagaag gcactcatgg
    13201 agtaccttct gctgggccgg cagagttgac gcagggacgc tcggcacgcc tgcgttattg
    13261 atcaaaatcg gccccagcgc ttgaccagca agcgcgttaa gctatcatta ttgaagcaat
    13321 cgctgatcta cgctgatcag ctctcactgc accgcgaact tggtccccgc cacgcgaatg
    13381 ccccagatca gctcgaccag cgcgcggttg ttgagcagcg gctcgccgcc gacgatcagc
    13441 caatagcccg tgccatcgac ccagaacagc atgaaggtgg tggtgggcat cagcatgtcg
    13501 atgccctcat ggaacgacac cgcccggccc gtgcccttga agccgcgctg cttctggcgc
    13561 gccacgaagt cgaagaagtc cgccgcggcc acgcacagcg tgtcggcctc gtcctgagaa
    13621 tagcccacgc gcgccaggca gaagccgtct tcggacgcca gcgcggccat gcgcgagccc
    13681 gacaagccgg caatggcgtg cggcaggtag ttgtccaggc gcacgtcggg tgcctgcaat
    13741 tcgcgaccca cctcgtgcac ccagccctcg tgcagggcac gcttgagcag tatcgtgtgc
    13801 gacgccatct gcgccagcca cgcgctgcgg cgcagcgaag cgccccgcgg catcagcgtc
    13861 tgcagcgccg cgcaagtctc atccggcgcg cggtcggcgt acgcatgcag cgcacccgcc
    13921 ggcgtcagca tcacataggg atcgtcctgt tcttcgctca tggcttcgcg ctgccgttga
    13981 acgggtcgat gccgtagatc agtgccttga ccagcgtcgc catgtgcgcc cggctgcggg
    14041 cgtcggtgtc catggccgtg ggcggcaggc ccatggccag cagctcgctc gatacgcgct
    14101 ggcgcgtctg ccacccgccg gtatcggtgt gcgtgatgcc caccaccagc gaggtcttgt
    14161 cgatgaagtc gcgaaactcg gtgacgtagg tgcgcaggtc ggacaccgga tcctcggcat
    14221 tgcccttgag catcagcacc aggccgagcg cgttctcggt caggatgtcc cacatgaagt
    14281 cga
//
LOCUS       DAOMVO010000028        13921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_965_length_13921_cov_11.188374, whole genome shotgun sequence.
ACCESSION   DAOMVO010000028 DAOMVO010000000
VERSION     DAOMVO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_13921_cov_11.188374"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PKV30_02815"
     CDS             complement(<1..979)
                     /locus_tag="PKV30_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRL29184.1"
                     /translation="MLSGLRSKSWLVDLPLIALAWWAGFWLRFNLDVPQPYFDQAVLT
                     TPLAMACMAVGLAWARVPRQSWRYVSLADLRQLAKGVALGGLLTTALVMGLRVDGFPR
                     SVFLIGTVLTLLLLAGARAAWRTLTERARAQGTPGAAQQPLLIVGTLDEADQALRTLT
                     GVAGWQVVGIVSPHAQDAGRTLQGVRVLGGVQALPELARAQQAGTVLLASAPGAPLRR
                     EVMLRWSGAGLNLLTLPTADELLAPGSPALPRQVRLEDLLGRTPVQLDAQGLNGLFTG
                     QTVLVTGAGGSIGAELCRQVARFGVARLVCVDVSEYATYTLEQELQRSFP"
     gene            complement(1097..1708)
                     /locus_tag="PKV30_02820"
     CDS             complement(1097..1708)
                     /locus_tag="PKV30_02820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRL29185.1"
                     /translation="MTTPAHDLAKRAMDVALSLLALPLAVPLMALIALAVKLDSPGPV
                     WFRQERVGRAGRLFRIHKFRTMRVTDGSGPLVTASHDARITRVGRWLRRTKLDELPQL
                     IDVLNGDMSLVGPRPEVPRYVALYAEPVRRQILSLRPGITDRAAIEFRDEERLLAAAA
                     DPERAYVEQVMPIKQRYYLDYVAQRSLAGDLRILWDTARALVK"
     gene            complement(1705..2442)
                     /locus_tag="PKV30_02825"
     CDS             complement(1705..2442)
                     /locus_tag="PKV30_02825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL29186.1"
                     /translation="MGQVTSAPVQASANADAASDLACFGWSARAWAAPWRAFRRAHPD
                     LQVGDALELGAGEHSSLAPLLLPLAERVECSAYDAAVLPAIEARHRASLPPAEAARMH
                     WAQRDLHALAGRWDLIVMKSVLGGVHRTHNSRLADVHATLERLRTQHLRPGGWLLTLD
                     NGRTALEPLWSRFGARRNGWRFFAAEDFPPAAEHRSFGVFSSFSAATRLGALGQRIDD
                     ALYAADLALTPLARQHAVHLHVYRAPA"
     gene            complement(2450..3607)
                     /locus_tag="PKV30_02830"
     CDS             complement(2450..3607)
                     /locus_tag="PKV30_02830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRL29187.1"
                     /translation="MNPPAFLPFARPDITEAEIDAVARAMRSGWVTTGPETKAFEQEF
                     AAYLGGGVHAIAVNSATAGLHLALEAIGVGPGDEVIAPTLTFTATVEVARYLGADAKL
                     VDVDPVTLNIDPAQIEAAITPRTKAILPVHYGGLACDMAAIFAMAKKHDLQVIEDAAH
                     ALPTTVNGALIGQLASAATVFSFYANKTMTTGEGGMVVTKDEALAARMKVMRLHGISR
                     DAFDRFTSTTPAWYYEIVAPGFKYNMTDMAAALGRVQLQRLPGFVQRRQQLAARYLHE
                     LQALPLILPADAPAGDAHAWHLFVLRLADDARATRDEVIQALSDAGIATSVHYVPLHR
                     QPYWRERYQLTPEQFPVAEAAYQRMFSIPLFTAMSDDEQGRVIAALRAVVR"
     gene            complement(3661..4680)
                     /locus_tag="PKV30_02835"
     CDS             complement(3661..4680)
                     /locus_tag="PKV30_02835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL29188.1"
                     /translation="MSESQRVSLIAPCRNERDHVDAFCDSALAQQLPPGWQMELLVAD
                     GASDDGTRARLDARAAQDARLRVIDNPGRIVSTGLNAALAAARGAVIVRLDMHTVFAP
                     DYVAECLAALARSGADNVGGPWVARGQGAGGRAIAAAFQSRWVVGGARSRDAAYEGEA
                     DTVYLGCWPRATFERVGGFDEQLVRNQDDEHNLRLRRAGGRIWQSPRIRSWYVPRASL
                     AKLFAQQYQYGYWRPFVMLKHGQPGSVRQLVPALFVAALAGGALLAPWWPWPLVLLLI
                     SYALYLGGASLAAARQAGEGRLLARLPAVIAAYHLGYGAGTWRGLLDVLLRRRASERW
                     SRLTR"
     gene            complement(4677..5741)
                     /locus_tag="PKV30_02840"
     CDS             complement(4677..5741)
                     /locus_tag="PKV30_02840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL29189.1"
                     /translation="MTISPHLVLLGDATSVHVQRWAREMGARGWRVSLVTARPQPIDG
                     VEQVTLPPVARSSDWLWRVGAARRAIAALAPDIVHAHYVTSYGYLAARCGHQPLVMTA
                     WGSDLLLTPRQNPLIARLTAWTLGRASLVTGDSRDLLDAARRLCPGVRVELIHWGVER
                     ARFAPVPWADKPGFEAVSLRSWEPNYRIDVILRAFAQVLVQRPDARLHLLGGGGEEGR
                     LRRLVDGLALQNSVQFHGRLDDAGMAAVLARCKLSISVPESDATSVSVLESMACGLAV
                     IATDLPANRDWLDARALVPPGDVVALARGWSGLIGDGALAQHMGRDNAERIAQGGDRR
                     VQMDAMDALYRQLLASKARA"
     gene            complement(5806..5928)
                     /locus_tag="PKV30_02845"
     CDS             complement(5806..5928)
                     /locus_tag="PKV30_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29190.1"
                     /translation="MNHFFERKGRQDFAKGAGVLYEKPIQRLLDERKKLCFLKQ"
     gene            complement(5929..6477)
                     /locus_tag="PKV30_02850"
     CDS             complement(5929..6477)
                     /locus_tag="PKV30_02850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRL29191.1"
                     /translation="MTALRAWVNRWRRRAWRVLFFRVLFGERSGGQLLAGTRISPSTC
                     IEHEDQLTLADDVFIGHFNFIEASAGVSIGRGTQVTNFVSIVSHSTHRAVRVAAELSG
                     AELDAAVVRGPISIGERCFIGPHSTIEAGSTLGHGTLVAGHSRVRGSFPDFAVLSGRP
                     ARVVGDVREGDERWLRELRGSR"
     gene            complement(6474..7754)
                     /locus_tag="PKV30_02855"
     CDS             complement(6474..7754)
                     /locus_tag="PKV30_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRL29192.1"
                     /translation="MTTARAPSLAGATLTLLWGGALAQLLPLLLGPVIARLFTPAAMG
                     VFTQFTTIAAALAVAASLRYEQALPLALDDADAAALLALALRLVAGAVLVSVPLAWAL
                     HAAGWLPLPALLPLAMACSGLLQVLMLWANRAQRFGALAVSRVAQYGGAALGQVLLGW
                     LLWQGAAAGRDGAWALVLAPMAAQLLAMAWLLRPAPAGGWRALRAPAAPALRERMGAM
                     ARQHKDFALVNTPHAFLGTLQDALAVALLVAWSGEAAAGFWGLALRYLKAPATLVGSA
                     VSQALYPRLAAASADEAPRLVRQVMALLALPALGLMALLLVAGPWLFERLFGAPWREA
                     GELARALAPYIAAHFVAAPLAVVTMAWDAQRWAFRLALVGQALFVAALAIGLMQGGLI
                     GGAWAVSAVMVPYFGWYFWRLARWPQVPARQVGA"
     gene            7928..9466
                     /gene="lysS"
                     /locus_tag="PKV30_02860"
     CDS             7928..9466
                     /gene="lysS"
                     /locus_tag="PKV30_02860"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRL29193.1"
                     /translation="MNQPAAPQAPEAQDENKLIAERREKLKTLREGGAVAFPNDFKPK
                     DRAAQLHQAHAAIEKEAFDADIAAGKKIAASIAGRMMLKRVMGKASFATLQDATGRIQ
                     AYITKEDVGEATYDAFKHWDLGDIVAVEGHMFRTGKGELSIHATSIRLLTKSLRPLPD
                     KFHGMQDQELKYRQRYVDLIMDEAARARFAARSATLAAMRGFMTDNGFMEVETPMLHP
                     IPGGANAKPFVTHHNALDQQMFLRIAPELYLKRLIVGGFERVFEINRSFRNEGISVRH
                     NPEFTMMEFYAAYWNYQDLMSFTEDLLRDTAQRAVGSLQLSYQGRDVDLRQPFARMTI
                     REAIHAHTDIGAHVDDAAELISRLKKLGLSEEKDKLSARSLPSLQVLYFEEAVESELW
                     APTFIMEHPTEISPLARANDARPEVTERFELYITGREMANGFSELNDAEDQAARFSAQ
                     ANAKDAGDDEAMFYDADFIRALEYGMPPTGGCGVGIDRFMMLLTDSPSIRDVILFPAL
                     RHEA"
     gene            complement(9527..11230)
                     /locus_tag="PKV30_02865"
     CDS             complement(9527..11230)
                     /locus_tag="PKV30_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29194.1"
                     /translation="MAATDSAAFTRARDFLLAHRTDYDTAYRDFRWPQLTEFNWALDH
                     FDPLARGNDATALHIVEEDGRETRRSFASLSARSDQVANWLRAQGVERGDRVLLMLGN
                     ELALWELMLACIKLGAVMIPATMLLTPEDLRDRLERGGVKHVAVASEQAAKFADLPGR
                     YTRITVGAAVPGWLRFEDSASAPTAFTPDGPTQATDPLLLYFTSGTTSKPKLVLHTHQ
                     SYPVGHLSTMYWIGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGAAIFIYNYARFSAP
                     ALLGVLEKYKVNSLCAPPTVWRMLIQEDLAAWKDRLALREVIGAGEPLNPEIIDRVQD
                     AWGLTLRDGFGQTETTAQIGNPPGLPVKAGSMGRPLPGYEVVLLDVDDNPAAEGELAL
                     NLALRPLGLMVCYEDSPEKTAEVMRQGCYRTGDTATRDADGYITFVGRADDVFKASDY
                     RISPFELESCLIEHPAVTEVAIVPSPDPVRLAVPKAFVTLAPGQQPSPELAQAIFGFA
                     RERLAPYKRVRRLQFVTELPKTISGKIRRVQMRKQESERGEGARVQHEYFEDDFPSFR
                     N"
     gene            complement(11420..11812)
                     /locus_tag="PKV30_02870"
     CDS             complement(11420..11812)
                     /locus_tag="PKV30_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29195.1"
                     /translation="MSYQSLPPPSASAANGLDRLFARVMGWVVWGVLALMGLVFALSL
                     FVWLTAMVLVSLVASVFTGRPAAVTMLWRRYRAMTQGRWPQRQPASSTPRADAAQSTG
                     ASMPTGVQDVRWREVAPASEAADDARHP"
     gene            complement(11879..12934)
                     /gene="nagZ"
                     /locus_tag="PKV30_02875"
     CDS             complement(11879..12934)
                     /gene="nagZ"
                     /locus_tag="PKV30_02875"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRL29196.1"
                     /translation="MQHAPLIIDIAGTALTDDDRRRLAHPLVGGLILFARNWQSRAQL
                     SQLSADIKRLQPDLLICVDHEGGRVQRFRADGFTHLPPMRALGELWMQDAMRAQDAAS
                     ACGYVLAAELRACGVDFSFTPVLDLDWGPSAVIGDRAFHADARVVTMLAKSLTHGLLR
                     AGMAHCGKHFPGHGFTAADSHTDAPRDTRSLKAILQDDAAPYGWLGSTLTAVMPAHVI
                     YPKVDSRPAGFSARWLQAILRGRLGFGGAIVSDDLSMEGARRIDGRTLTPTEAVLAAL
                     DAGCDLALLCNQSLGNGSVIDDTIDGLAQAQLSGRWQPRPASEARRLALLPAAPAADW
                     DTLMRTPDYLRALALIP"
     gene            complement(13003..13410)
                     /gene="acpS"
                     /locus_tag="PKV30_02880"
     CDS             complement(13003..13410)
                     /gene="acpS"
                     /locus_tag="PKV30_02880"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRL29197.1"
                     /translation="MIHGIGTDICDIRRIRASFERHGERFAQKILSDAELAAWRRRSA
                     RWPDRGVRYLATRFSAKEAFSKAIGLGMRLPMHWRLCEIANRPSGEPFIVLHGGLKDW
                     FDAQHLMAHITVTDESEYAASFCVVEKTARDFK"
     gene            complement(13470..>13921)
                     /locus_tag="PKV30_02885"
     CDS             complement(13470..>13921)
                     /locus_tag="PKV30_02885"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRL29198.1"
                     /translation="FTSDHGWDLADAAVRERLRPLIEECRALAVRVSLFMDPSPELMV
                     WANEVGADRVELYTEPYAAAWADPAARQPQLIAYARAAQAALSVGLGVNAGHDLNRDN
                     LTEFLRAVPGVAEVSIGHALIADALELGYAATVSEYLRCIAEAYKDS"
BASE COUNT         2330 a         4866 c         4716 g         2009 t
ORIGIN      
        1 gcggaaagct gcgctgcagt tcctgctcca gcgtgtaggt ggcgtactcg gacacatcca
       61 cgcacaccag cctggcgacg ccaaagcgcg ccacctggcg gcacagctcg gcaccgatgg
      121 agccgccggc gccggtcacc agcacggtct ggccggtgaa caagccgttc aggccctgcg
      181 cgtcgagctg caccggcgtg cggcccagca aatcttccag ccgcacctgg cgcggcaggg
      241 ccgggctgcc gggcgccagc aactcgtcgg ccgtgggcag cgtcagcagg ttcaggccgg
      301 cgccggacca gcgcagcatc acctcccgcc gcagcggcgc gccgggcgcg ctggccagca
      361 acacggtgcc ggcctgctgc gcgcgcgcca gctcgggcag cgcctgcacg ccgcccagca
      421 cgcgcacgcc ttgcagggtg cggccggcgt cctgcgcatg gggcgagaca atgcccacca
      481 cctgccagcc ggccacgccg gtcagcgtgc gcagcgcctg gtcggcctcg tccagcgtgc
      541 cgacgatcaa cagcggctgc tgcgccgcac ccggcgtgcc ctgggcgcgc gcgcgttcgg
      601 tcagggtgcg ccaggcggcg cgcgcgccgg ccagcagcag cagcgtgagc accgtgccga
      661 tcagaaacac cgagcgcgga aagccatcga cccgcaagcc catgaccagc gcggtggtca
      721 gcaggccgcc cagcgccacg cccttggcca gttggcgcag atcggccagg ctgacgtagc
      781 gccacgactg gcgcggcacg cgcgcccacg ccaggccgac cgccatgcac gccatggcga
      841 gcggcgtggt cagcaccgcc tggtcgaaat aaggctgtgg cacgtcgaga ttgaagcgca
      901 gccagaagcc ggcccaccag gccagcgcga tgagcggcaa atcgaccagc cagctcttgc
      961 tgcgcaggcc gctcagcatg tgatctcctc ggagcgcatt ctgttcatta ctctcttttt
     1021 catagctgcc cgcgcttgat tggcaagcgc cagtggccga ttttggcaag aagcgcttgc
     1081 acgcgggcga cagcggtcat ttcaccagcg cccgcgccgt gtcccacaga atgcgcaggt
     1141 cgccagccag gctgcgctgc gccacatagt cgaggtaata gcgctgcttg atgggcatca
     1201 cctgctcgac gtaggcgcgc tccgggtcgg cggcggcggc cagcaaacgc tcttcgtcgc
     1261 ggaactcgat ggcggcgcgg tcggtgatgc ccgggcgcag gctgagaatc tggcggcgta
     1321 cgggttcggc gtacagcgcc acgtagcgcg gcacctcggg ccgcggaccg accaggctca
     1381 tgtcgccgtt gagcacgtcg atcagctgcg gcagctcgtc gagcttggtg cgccgcagcc
     1441 agcggccgac gcgcgtgatg cgtgcgtcat gcgacgccgt gaccagcggc cccgaaccat
     1501 ccgtcacgcg catggtgcgg aacttgtgga tgcgaaacag ccgcccagca cgccccacgc
     1561 gctcttgccg aaaccacacc gggccgggtg aatccagctt gaccgccagc gcgatgagcg
     1621 ccatcagcgg caccgccagc ggcagggcga gcaggctgag cgcaacgtcc atcgcgcgct
     1681 tggccaggtc gtgcgccggc gtggtcatgc cggcgcgcgg tacacgtgca ggtgcacggc
     1741 gtgctggcgc gcgagcggcg tcagcgcaag gtcggcggcg tacaaggcgt cgtcgatgcg
     1801 ctggcccaaa gcgcccagcc gcgtggcggc ggaaaagctg ctgaaaacgc cgaagctgcg
     1861 gtgctcggcc gccggcggaa aatcttcggc cgcgaaaaaa cgccagccgt tgcggcgcgc
     1921 gccaaagcgc gaccacagcg gctccagcgc ggtgcggccg ttgtcgagcg tcagcaacca
     1981 gccgccgggg cgcaggtgct gagtgcgcag gcgttccagc gtggcatgca cgtcggccag
     2041 gcggctgttg tgcgtgcggt gcacgccgcc cagcaccgac ttcatgacga tcaaatccca
     2101 gcgcccggcc agcgcatgca ggtcgcgctg cgcccagtgc atgcgcgccg cttcggccgg
     2161 tggcaggctg gcgcggtgcc gggcctcaat cgcgggcagc accgctgcgt cgtaggccga
     2221 acactcgacc cgctcggcca agggcagcag cagcggtgcc agcgaagaat gttcgccggc
     2281 gcccagttcc agcgcatcgc ctacttgcag atcggggtgc gcccggcgga aagcgcgcca
     2341 cggcgcggcc caggcgcgcg cgctccagcc gaagcacgcc agatccgacg cggcgtcggc
     2401 gttcgctgat gcctggactg gcgcgctcgt cacctgaccc accatgcgct caacgcacca
     2461 ccgcccgcaa ggcggcaatc acgcggccct gctcgtcgtc gctcatcgcg gtgaacagcg
     2521 ggatgctgaa catgcgctgg taggccgctt cggccaccgg gaactgctcg ggcgtcaatt
     2581 ggtagcgctc acgccagtac ggctggcggt gcagtggcac gtagtgcacg ctggtggcaa
     2641 tgccggcgtc gctcaaggcc tgaatcactt cgtcgcgcgt tgcccgcgcg tcgtccgcca
     2701 gccgcagcac gaacaggtgc cacgcgtgtg catcgcccgc cggggcatcg gccggcaata
     2761 tcagcggcaa ggcctgcaac tcgtgcaggt agcgcgcagc cagttgctga cggcgctgca
     2821 cgaagcccgg caggcgctgc aactgcacgc ggcccagtgc cgcggccatg tcggtcatgt
     2881 tgtacttgaa gccgggcgcg acgatttcgt agtaccacgc gggtgtggtg cttgtgaagc
     2941 ggtcgaacgc gtcgcggctg atgccgtgca ggcgcatcac cttcatgcgg gcggccagtg
     3001 cttcgtcctt ggtcaccacc atgccgcctt cgccggtggt catggtcttg ttggcgtaaa
     3061 agctgaatac cgtggcggcg cttgccaatt gaccaatcag cgctccatta actgtagtgg
     3121 gcagcgcgtg cgccgcgtcc tcgattacct gaaggtcgtg ctttttcgcc atggcaaaga
     3181 tcgctgccat gtcgcacgcc agtccgccgt agtgcaccgg caggatggct ttcgtccgcg
     3241 gcgtgatggc agcttcgatc tgcgccggat cgatgttgag cgtgacagga tcgacatcca
     3301 ccaatttcgc atcggcaccg aggtagcgcg ccacctccac cgtggcggtg aaggtcagcg
     3361 tcggcgcgat cacttcgtcg ccggggccga cgccgatggc ttccagcgcc agatgcaggc
     3421 cggcggtggc gctgttgacg gcgatggcgt gcacgccgcc gcccaggtag gcggcaaatt
     3481 cctgctcgaa cgctttcgtc tccggccccg tcgtcaccca gcccgagcgc atggcgcgcg
     3541 ccacggcatc gatttcggcc tcggtgatgt cggggcgggc aaaaggcaga aaagcgggtg
     3601 ggttcatgat caaaaatgcc gccagcgctt gtccatcaag cgcaagcagc tatgattttg
     3661 ttagcgcgtc aggcggctcc agcgctcgct ggcgcggcgg cgcagcagca cgtcgagcag
     3721 gccgcgccag gtgcccgcgc cgtagccgag atgataggcg gcgatcaccg ccggcaagcg
     3781 cgcgagcaaa cgcccctcgc ccgcctgccg cgccgccgcg agcgatgcgc cgccgaggta
     3841 cagcgcgtag ctgatcagca gcaagaccag cggccacggc caccacggcg ccagcagcgc
     3901 gccgcccgcc agcgcggcca cgaacagcgc cggcaccagt tggcgcaccg atcccggctg
     3961 cccatgtttg agcatcacga acggccgcca gtagccgtac tggtattgct gcgcaaacag
     4021 cttggcgagc gacgcgcgcg gcacgtacca cgagcggatg cgcgggctct gccagatgcg
     4081 gccgccggcg cggcgcaggc gcaggttgtg ctcatcgtcc tggttgcgca ccagttgctc
     4141 gtcaaagccg cccacgcgct cgaacgtggc gcgcggccag cagccgagat acacggtgtc
     4201 ggcctcgccc tcatacgccg catcgcgcga gcgcgcgccg cccaccaccc agcggctttg
     4261 aaaggcggcg gcaatcgccc ggccccccgc gccctgcccg cgtgcgaccc acgggccgcc
     4321 cacgttgtcg gcgcccgagc gcgccagcgc ggccaggcat tccgccacgt agtcgggtgc
     4381 gaacacggtg tgcatgtcca gccgcacgat gactgcgccg cgcgcggcgg ccagcgcggc
     4441 gttgaggccg gtcgagacga tgcggccggg gttgtcaatg acgcgcaggc gcgcatcctg
     4501 cgcagcgcgg gcgtcgagcc gcgcgcgcgt gccgtcgtcg cttgcaccgt cggccaccag
     4561 cagttccatc tgccagccgg gcggcagttg ctgggcgagt gcgctgtcgc agaaggcatc
     4621 gacgtggtcg cgctcgttgc ggcagggggc gatgaggctg acgcgttgcg actcgctcat
     4681 gcgcgcgcct tcgacgccag cagttggcgg taaagcgcat ccatggcgtc catttgcacg
     4741 cgacggtcgc caccctgcgc aatacgttcg gcgttgtctc ggcccatgtg ctgggcaagc
     4801 gccccgtcgc cgatcagccc agaccatcca cgcgccagcg ccacgacgtc accgggtggc
     4861 accagcgccc gcgcatccag ccaatcgcgg ttggccggca gatcggtggc aatcacggcc
     4921 agcccgcagg ccatgctttc aagcaccgac accgaggtgg catcactctc cggcacgctg
     4981 atcgacagct tgcaccgcgc cagtacggcg gccatgccgg cgtcatccag ccggccgtga
     5041 aattgcactg aattttgtag cgccagaccg tctaccagcc tgcgcaagcg gccttcttca
     5101 ccgccaccgc ccagcaggtg caggcgcgcg tccggccgct gcaccaagac ctgggcgaag
     5161 gcgcgcagga tcacgtcgat gcggtagttc ggctcccaac tgcgcaggct gacggcctcg
     5221 aaacccggct tgtcggccca gggcaccggc gcgaagcgcg cgcgctcgac gccccaatga
     5281 atcaactcga cgcgcacgcc cgggcacagg cgccgggcgg cgtccagcag gtcgcgcgaa
     5341 tcgcccgtca ccaggcttgc gcgacccagc gtccaggccg tgagccgcgc gatcaacggg
     5401 ttctggcgcg gcgtgagcag caagtcgcta ccccaggccg tcatcaccag cggctggtgg
     5461 ccgcagcgcg ccgccagata gccgtacgag gtgacgtaat gcgcgtgcac gatgtccggc
     5521 gccagcgcgg cgatggcgcg ccgcgcggcg cccacgcgcc acagccagtc gctggagcgg
     5581 gccacgggcg gcagcgtgac ctgctcgacg ccgtctatcg gctgcggccg cgccgtgacc
     5641 aacgacacgc gccagccgcg cgcccccatc tcgcgcgccc agcgctgcac gtgcacgctg
     5701 gtggcgtcgc cgagcaggac gaggtggggc gaaatggtca tggcagcggc gaaacccttt
     5761 gaacgcaaag ggcgcagagg tttcgcaaag aacgcagaaa aaatctcatt gcttcaaaaa
     5821 acagagcttc ttgcgctcat ccagcaagcg ctggatgggt ttttcatata aaactcctgc
     5881 gccttttgcg aaatcctggc gccctttgcg ttcaaaaaaa tggttcacct atcgactgcc
     5941 tcgcaactcg cgcagccacc gctcgtcccc ctcgcgcaca tcgcccacca cgcgcgccgg
     6001 gcggccggac agcacggcaa agtccgggaa gctgccacgc actcggctgt gcccggccac
     6061 cagcgtgccg tggcccagcg tgctaccggc ttcgatggtg ctgtgcgggc cgatgaagca
     6121 gcgctcgcca atgctgatcg gcccgcggac cacggcggca tccagctcgg cgcccgacag
     6181 ctccgccgcc acccgcaccg cgcggtgcgt gctgtggctg acgatgctga cgaagttggt
     6241 cacctgcgtg cctcgcccga tgctcacgcc cgcgctggct tcgatgaagt tgaagtggcc
     6301 gatgaacacg tcgtcggcca aggtcagttg atcttcgtgc tcgatgcagg ttgaaggtga
     6361 gatgcgcgtg ccggccaaca actggccgcc gctgcgctcg ccaaacagca cgcggaagaa
     6421 cagcacccgc cacgcgcggc ggcgccagcg gttgacccac gcgcgcagcg cggtcatgcc
     6481 ccaacctgcc gcgccggcac ctgcggccag cgggccagcc gccagaagta ccagccgaag
     6541 tacggcacca tcaccgccga cacggcccac gcgcccccga tcaagccgcc ctgcatcagc
     6601 ccgatggcca gcgccgccac gaacagcgcc tgccccacca gcgccagccg aaacgcccag
     6661 cgctgcgcgt cccacgccat ggtgaccacc gccagcggcg cggcgacgaa atgcgcggcg
     6721 atgtagggcg ccagtgcgcg cgccagctcg ccagcttcgc gccacggcgc gccaaaaagc
     6781 cgttcgaaca gccacgggcc cgcgaccagc agcagcgcca tcaagcccag cgcgggcaga
     6841 gccagcagcg ccatcacctg ccgcaccagg cgcggcgcct cgtctgccga agccgccgcc
     6901 aggcgcgggt acagcgcttg tgacaccgcg ctgcccacca gcgtggccgg cgccttcaga
     6961 tagcgcagcg ccaaacccca gaaacccgct gccgcctcgc ccgaccaggc gacgagcagc
     7021 gccaccgcca gcgcatcctg cagcgtgccc aaaaaagcgt gtggcgtgtt caccagcgcg
     7081 aaatccttgt gctgccgcgc catcgcgccc atgcgctcac gcagcgccgg tgcggccggt
     7141 gcccgcaggg cgcgccagcc gccagcaggc gcgggccgca gcagccacgc catcgccagc
     7201 agctgcgccg ccatcggcgc cagcaccagc gcccacgcgc cgtcgcgccc ggctgccgcg
     7261 ccctgccaca gcagccagcc gagcagcacc tgcccgagcg ccgcgccgcc gtattgcgcc
     7321 acgcggctga cggccagcgc gccaaaacgc tgtgcgcgat tcgcccacag catcagcacc
     7381 tgcagcaaac cgctgcaggc catggccagc ggcagcagcg cgggcagcgg cagccagccg
     7441 gccgcgtgca gcgcccacgc cagcggcacg ctgacgagca cggcacccgc caccagccgc
     7501 agcgccagcg ccagcagcgc agcggcgtcg gcatcgtcga gcgccagcgg cagcgcctgc
     7561 tcataacgca ggctggccgc cacggccaac gcggcggcga tggtggtgaa ttgggtgaaa
     7621 acgcccatcg ccgccggcgt gaacaaacgc gcgatgaccg ggccgagcag cagcggcagc
     7681 agctgtgcca gcgcgccgcc ccacagcagc gtgagcgtgg ccccggccag cgacggcgcg
     7741 cgcgcggtgg tcaacgaccg gccctcgcgc ggaacgctgc gggcgttaaa gctggatgca
     7801 gtggaatatg gaacaagggt cgcccggtgg cgcgccttca ctaaaatggc gcgttttgcc
     7861 ctcacaacgc agggccgccg cgattatccc cgccagcggc tgccgccacc ttcacccgcc
     7921 ccccaccatg aaccagcccg ccgccccgca agcccccgaa gcccaagacg aaaacaagct
     7981 catcgccgag cggcgcgaaa aactgaaaac cctgcgtgaa ggtggcgcgg tcgccttccc
     8041 caacgacttc aagcccaaag accgcgcagc gcagctccat caagcgcacg cagctatcga
     8101 aaaagaagcg ttcgatgccg acatcgctgc cggcaagaag atcgccgcca gcatcgccgg
     8161 ccgcatgatg ctaaagcgcg tgatgggcaa ggccagcttt gccacgctgc aagacgccac
     8221 cggccgcatc caggcctaca tcaccaagga agacgtgggc gaggccacct acgacgcctt
     8281 caaacattgg gacttgggcg acatcgtggc ggtcgagggc cacatgtttc gcaccggcaa
     8341 gggcgagttg tcgatccacg cgacgtcgat ccggctgctg accaaaagcc tgcgcccgct
     8401 gcccgacaag ttccacggca tgcaggatca ggaactgaag taccgccagc gctatgtcga
     8461 tctgatcatg gacgaggccg cgcgcgcccg ctttgccgcc cgcagcgcca cgctggccgc
     8521 catgcgcggc ttcatgaccg acaacggctt catggaagtg gaaacaccca tgctgcaccc
     8581 cattccgggc ggcgccaacg ccaagccgtt tgtcacgcac cacaacgcgc tggatcagca
     8641 gatgttcctg cgcatcgcgc ccgagctgta tttgaagcgc ctgatcgtcg gcggctttga
     8701 gcgcgtgttc gagatcaacc gcagctttcg caacgaaggc atcagcgtgc ggcacaaccc
     8761 cgagttcacg atgatggagt tctacgcggc gtactggaac taccaggatt tgatgagctt
     8821 caccgaagac ctgctgcgcg acacggcaca acgggccgtg gggtcgcttc agttgtcgta
     8881 tcagggccgt gatgtggact tgcgccagcc gttcgcgcgc atgaccatcc gcgaggcgat
     8941 ccatgcgcac accgacatcg gcgcgcacgt cgatgacgca gctgaactca tcagccgctt
     9001 gaaaaagctg ggcctgagcg aagaaaaaga caagctttca gcgcgcagcc tgcccagcct
     9061 gcaggtgctg tacttcgagg aagccgtgga gtccgagctt tgggcgccca ccttcatcat
     9121 ggaacacccg accgagatca gcccgctggc gcgcgccaac gatgcgcggc ccgaggtgac
     9181 cgagcgcttt gagctgtaca tcaccggccg cgagatggcc aacggcttct cagagctgaa
     9241 cgacgccgaa gaccaggccg cccgcttctc cgcccaggcc aacgccaagg acgcgggcga
     9301 cgacgaggcc atgttctacg acgccgactt catccgcgcg ctcgaatacg gcatgccgcc
     9361 caccggtggc tgcggcgtgg ggattgatcg cttcatgatg ctgctgacgg attcgccgag
     9421 catccgggac gtgattctct tccccgcgct ccggcacgag gcttgagcct gatcgctcca
     9481 tttttcatag ctactggcgc acagtggacg tgcgccagcg cccaaatcag ttgcgaaagc
     9541 ttggaaaatc gtcctcgaaa tattcgtgct gcacccgcgc gccctcgccg cgctcgctct
     9601 cctgcttgcg catctgcacg cggcgaatct tgccgctgat ggtcttgggc aactcggtca
     9661 caaactgcag gcggcgcacg cgcttgtagg gcgccaggcg ctcgcgcgcg aagccgaaga
     9721 tggcctgcgc cagctcgggt gacggctgct ggccgggcgc cagggtgacg aaggccttgg
     9781 gcacggccag gcgcaccggg tcggggctgg gcacgatggc cacttcggtc accgccgggt
     9841 gttcgatcag gcagctttcc agctcgaacg ggctgatgcg gtagtcgctg gccttgaaca
     9901 cgtcatcggc gcggccgaca aaggtgatgt agccgtcggc gtcgcgcgtg gcggtgtcgc
     9961 cggtgcggta gcagccctgg cgcatgactt cggcggtctt ctcggggctg tcttcgtagc
    10021 agaccatcag gccgagcggg cgcagcgcca ggttcagcgc caactcgccc tcggccgccg
    10081 ggttgtcgtc gacgtcgagc agcacgacct catagcccgg cagcgggcgg cccatggagc
    10141 cggccttgac cggcaagccg ggcgggttgc cgatctgcgc cgtggtctcg gtctggccga
    10201 agccgtcgcg cagcgtgagg ccccaggcgt cttgcacgcg gtcgatgatc tcggggttga
    10261 gcggctcgcc ggcgccgatg acttcgcgca gcgccaggcg atccttccaa gcggccagat
    10321 cttcctggat cagcatgcgc cacacggtgg gcggcgcgca caggctgttg accttgtatt
    10381 tttccagcac gcccagcagc gccggcgccg aaaagcgcgc gtagttgtag atgaagatgg
    10441 cggcgcccgc attccagggc gcgaaaaagc agctccacgc atgcttggcc cagcccggcg
    10501 atgagatgtt gaggtgcacg tcacccggct gcagccctat ccagtacatg gtgctgagat
    10561 gccccaccgg gtagctctgg tgcgtgtgca gcaccagctt cggtttcgac gtggtgccgc
    10621 tggtgaagta gagcagcagc gggtcggtcg cctgcgtggg gccgtcgggc gtgaaggcgg
    10681 tgggcgcgct ggcgctgtct tcaaagcgca gccagccggg cacggcggcg ccgacggtga
    10741 tgcgcgtgta gcgcccgggc aggtcggcga acttggcggc ctgctcgctg gccacggcca
    10801 cgtgcttgac gccgccgcgc tccagccggt cgcgcaggtc ttcgggcgtc agcagcatgg
    10861 tggcggggat catcacggcg ccgagcttga tgcaggccag catcagctcc cacagcgcca
    10921 actcgttgcc gagcatcagc agcacgcggt cgccgcgctc gacgccttgg gcacgcagcc
    10981 agttggcgac ctggtcggag cgtgcactta agctcgcaaa gctgcgccgc gtctcgcgcc
    11041 cgtcttcctc gacgatgtgc agtgcggtgg cgtcgttgcc gcgcgccagg gggtcgaagt
    11101 gatcgagcgc ccagttgaac tcggtcagtt gcggccagcg gaagtcgcgg taggcggtgt
    11161 cgtagtcggt gcggtgcgca agcagaaagt cgcgcgcccg cgtgaacgcg gcgctgtcgg
    11221 tggcggccat ggtcttgtct ccggtgttgt cgtgcaacgg tacggcggcg caccctgcgc
    11281 gtgggccgac cccggaatca tcttagccga gcggatgagg cgatgtagag cgtgggctca
    11341 ccgcgccgcc tgatttcgca tcaaacaggc cgccagcgct ttcccatcaa gcactggcag
    11401 ctctcaaaac gagagcgaat caggggtgac gcgcgtcgtc ggccgcttca ctggcgggcg
    11461 ccacttcgcg ccagcgcacg tcctgcacgc cggtgggcat ggaggcgccg gtgctttgcg
    11521 cggcgtcggc gcgcggggtg ctgctggcgg gttggcgctg cggccagcgg ccttgcgtca
    11581 tggcgcggta gcggcgccac agcatggtca cggcggcggg gcggccggtg aacacgctgg
    11641 caaccaggct gaccagcacc atggccgtca gccagacgaa caggctgagc gcaaacacca
    11701 gccccatcag cgcgagcacg ccccacacca cccagcccat gacgcgggcg aacaggcgat
    11761 ccagcccgtt ggcggcgctg gcggaaggcg gtggcaggct ttggtaagac atgggattca
    11821 tgcccagtca gattgcgcgg ggtggcgaaa gttcatctgc cgccattttg gcgccttttc
    11881 agggaatgag cgccagcgcg cgaaggtaat ccggcgtgcg catcagggtg tcccaatcgg
    11941 cggccggcgc ggcgggcagc agcgccaggc ggcgcgcctc gctggccggt cggggctgcc
    12001 agcggccgct gagctgcgcc tgggccagac cgtcgatggt gtcgtcgatc acgctgccgt
    12061 tgccgagcga ctggttgcac agcagcgcca ggtcacaccc ggcgtcgagc gcggccagca
    12121 cggcttcggt cggcgtcagc gtgcggccgt cgatgcggcg cgcgccttcc atgctcaaat
    12181 cgtcgctgac gatggcgccg ccaaagccga gccgcccgcg caggatggcc tgcagccagc
    12241 gcgcagaaaa gccggcgggg cgactgtcga ccttgggata gatcacatgc gccggcatga
    12301 cggcggtgag cgtgctgccc agccagccgt agggcgcggc gtcgtcctgc aagatcgcct
    12361 tgagcgaacg cgtatcgcgc ggcgcgtcgg tgtgcgaatc ggcggccgtg aagccgtgac
    12421 caggaaagtg tttgccgcaa tgcgccatcc cggcgcgcag caggccgtgc gtcaggcttt
    12481 tggcgagcat cgtcaccacg cgcgcatcgg cgtgaaaggc acggtcgcca atgacggcgc
    12541 tcgggcccca gtcgagatcg agcacggggg tgaagctgaa atcgaccccg caggcgcgca
    12601 gctcggccgc cagcacgtaa ccgcaggcgc tggccgcgtc ctgcgcgcgc atggcgtcct
    12661 gcatccacaa ctcgccgagc gcgcgcatgg gcggcaggtg cgtgaagcca tcggcgcgaa
    12721 agcgctgcac gcggccgcct tcgtgatcga cgcagatcag cagatcgggc tgcaggcgct
    12781 tgatgtcggc gctgagctgc gacagttgcg cccggctttg ccagttgcgc gcaaacagga
    12841 tcaggccgcc gaccaacgga tgcgccaggc gccggcggtc gtcgtcggtc aacgcggtgc
    12901 cggcgatgtc gatgatgagg ggggcgtgct gcatgttgaa ggtcagatca ctgcgttttt
    12961 aatagctgct tgcgcttgtc ctacaagcgc tggggccggt tttcatttga aatcgcgcgc
    13021 cgtcttctcg accacgcaaa agctcgcggc gtattcgctc tcgtccgtca cggtgatgtg
    13081 cgccatcagg tgctgggcgt cgaaccagtc cttcagcccg ccgtgcagca cgatgaaggg
    13141 ctcgccgctc ggccggttgg cgatttcgca cagccgccag tgcatgggca ggcgcatccc
    13201 caggccaatc gccttgctga acgcctcctt ggcggaaaag cgcgtggcca gataacgcac
    13261 gccgcgatcc ggccagcgcg cgctgcggcg gcgccaggcg gcgagttcgg cgtcggacag
    13321 gattttttgc gcaaagcgct cgccatggcg ctcgaagctg gcgcggatgc gacggatgtc
    13381 gcagatgtcg gtgccgatgc cgtggatcat tgggttggga aaaaaatcgg ccgctggcgc
    13441 ccgtccatca agcgcgggca gctatcaaat caagagtctt tatacgcttc ggcgatgcag
    13501 cgcagatact cgctcacggt ggccgcgtag cccagctcca gcgcatcagc gatcagcgca
    13561 tggccgatgg agacttcggc cacgccgggt acggcgcgca ggaattcggt gaggttgtcg
    13621 cgattcaggt cgtggccggc gttgacgcct agtccgacgc tgagcgctgc ctgcgcggcg
    13681 cgggcgtagg cgatcagttg cggctggcgc gcagctggat cggcccacgc ggcggcgtac
    13741 ggctcggtgt aaagctccac gcggtcggcg cccacctcgt tggcccagac catcagctcg
    13801 ggcgacggat ccatgaacag gctgacgcgc accgccaggg cgcggcattc ttcgatcagc
    13861 gggcgaaggc gctcgcgcac cgctgcgtcg gccaagtccc agccgtggtc gctggtgaac
    13921 t
//
LOCUS       DAOMVO010000029        13777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_978_length_13777_cov_16.041175, whole genome shotgun sequence.
ACCESSION   DAOMVO010000029 DAOMVO010000000
VERSION     DAOMVO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13777
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_13777_cov_16.041175"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKV30_02890"
     CDS             complement(<1..562)
                     /locus_tag="PKV30_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRL29199.1"
                     /translation="MKHAIRHIHFVGVGGSGMSGIAEVLHNLGYVISGSDLADSATLR
                     RLAGMGIRTFVGHAAANVEGADAVVTSTAVKPDNPEVLVARDKRIPVVPRAMMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASLLAEAGLDPTFVIGGRLNSAGANARLGSGDYIVVE
                     ADESDTSFLNLLPVMAVVTNIDADHME"
     gene            complement(559..1635)
                     /gene="murG"
                     /locus_tag="PKV30_02895"
     CDS             complement(559..1635)
                     /gene="murG"
                     /locus_tag="PKV30_02895"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRL29200.1"
                     /translation="MTQKCALVMAGGTGGHIFPGLAVADGLRARGWRVHWLGVPGSME
                     AQLVPPRGYAFEPVEFGGVRGKGVTTLFMLPLRLLKAFAQSIGVIRRVKPDVLVAFGG
                     YITFPGGLMGVALNKPLVLHEQNSIAGMANKVLAHIADRVFTAFPGVLPKARKVQWVG
                     NPLRESFKQVPDPAMRFACRSGPLKILVVGGSLGAQALNDVVPKALALIPAGQRPQVV
                     HQSGAKQIDALRANYDAAGVQAELVPFIDDMAKSYADADLVICRAGATTVTEIAAIGA
                     AALFVPFPHAVDDHQTTNAKFLVDQGAGWLVQQRDLTPERLAEMLQKTERSALVQRAL
                     AAKKMQKIHAVEDVVTACEELTAR"
     gene            complement(1632..2906)
                     /gene="ftsW"
                     /locus_tag="PKV30_02900"
     CDS             complement(1632..2906)
                     /gene="ftsW"
                     /locus_tag="PKV30_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRL29201.1"
                     /translation="MNAAADALNPPSRKKPRAARAKKRLADELPVRIGGMEYARTAST
                     PARVLGFDQALIWACVALLAWGLVMVYSASIALPDNPRFGRYSPNYFVFRHAMWIGIA
                     FVAALIAFQVRMDWWEKAAPWLFVVSLALLIAVLIPHVGSVVNGARRWIALGPMSFQP
                     SEVAKITVLLYAADYMVRKMEVKERFFRAVLPMGVAVGVVGALLLAQPDMGAFMVIAV
                     IAMGILFLGGVNARMFFIIAAFLVVAFALMIWASPWRRERIFAYLDPFSEDHALGKGY
                     QLSHALIAIGRGEVFGVGLGGSIEKLHWLPEAHTDFLLAVIGEEFGLVGLLCVIVLFF
                     WLTRRIMHIGRQAIALDRVFSGLVAQGIGLWLGFQAFINIGVNLGALPTKGLTLPFMS
                     YGGSAILANMVAIAIVLRVDVENRRLMHGGRT"
     gene            complement(2903..4429)
                     /gene="murD"
                     /locus_tag="PKV30_02905"
     CDS             complement(2903..4429)
                     /gene="murD"
                     /locus_tag="PKV30_02905"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRL29202.1"
                     /translation="MTMLQDRHVLVLGLGLSGLAMARWCAGQGAQVTVVDTREAPPQL
                     DALRQQVPAARFVHGAMDASLLDDASLHAVYRSPGLAPISIAGVVQAARERGLLVGGE
                     LDLFTQTLSNLKHTIGYEPALLAITGTNGKTTVTALTGQLVERAGRSVVLAGNIGPTL
                     LDTLQAHLVAETLPEVWVLELSSFQLDGVAGFEPTAATVLNITQDHLDWHGDMAAYAA
                     AKARIFGEHGVMVLNRDDAAVMAMRPAPPAKVPKGTPKPEPRTVLTFGADMPTEPGDF
                     GIELVGGMAWLVRALEADETQRRRKDAEVELHIQRLMPADALRIRGRHNAVNALAALA
                     LATSAGCALGPMLYGLREYRGEPHRVESIGLLDGVEYFDDSKGTNVGATVAALEGLGT
                     ERKLVVILGGDGKGQDFSPLAAPVARFVRAVVLIGRDAPLLRAALRDTGVPLLDATDM
                     PDAVAQAQARAHSGDAVLLSPACASLDMFKNYGERAEVFRAAVRDLALAAGTDLEVLG
                     "
     gene            complement(4434..5612)
                     /gene="mraY"
                     /locus_tag="PKV30_02910"
     CDS             complement(4434..5612)
                     /gene="mraY"
                     /locus_tag="PKV30_02910"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRL29203.1"
                     /translation="MLLSLATWLQALSPEFGFFRVFQYLTFRAVMAAMTALVIGIVAG
                     PWVIRKLTELKIGQPVRGYAMETHLSKSGTPTMGGVLILLSIAISTLLWFDWSNRFVW
                     IVMIVTFGFGAIGWADDWRKVVNKDPEGMRSREKYFWQSVIGLLAALYLAFAISGASN
                     VEVWALFVGWVSSGFTIDLPHAAGLLVPFFKEVSYPLGVAGFVILSYLVIVGSSNAVN
                     LTDGLDGLAIMPVIMVGSALGIFAYVTGSSVFSKYLLFPHIPGTGELLIFCAAMAGAG
                     LAFLWFNAHPAQVFMGDVGALALGGALGTIAVIVRQEIVLAIMGGIFVVEALSVMLQV
                     SYFKYTKRRFGQGRRLLKMAPLHHHFEKSGWAETQVVVRFWIITMLLCLVGLSSLKLR
                     "
     gene            complement(5602..7020)
                     /gene="murF"
                     /locus_tag="PKV30_02915"
     CDS             complement(5602..7020)
                     /gene="murF"
                     /locus_tag="PKV30_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRL29204.1"
                     /translation="MNAMNLTLGELAGWLAGARLIGDAGVRCDRVHTDTRTLRPGDLF
                     VALKGERFDAAAFLPDAAKQGAVAALCDASAEESLRASGLPGVLVPDARRALGELAHA
                     WRRRFDGPLIAVTGSNGKTTVTQMIASILCAWQGDAALATQGNFNNDIGVPLTLLRLR
                     PEHKVAVVELGMNHPGEIAQLAAIAQPTVALVNNAQREHQEFMASVQAVAEENGSVFT
                     ALGDGGTAVFPAGDLYQNTWKRLSGKRRQLLFGEQSDGAEVSLINAAWRAEHWQATAR
                     TPAGDVAFELHAAGRHNLRNALAAIACTTAAGAPLAAIAQGLSAFRPVKGRSRTLLLR
                     HRGRAITLVDDTYNANPDSVRAVIDVLAELPGPRLLVLGDMGEVGDQGPAFHAEVGAY
                     ARERGIERLLAHGPQAIHAAMAFGGGRHFDDMAALTDAVRTHLPQCTSIAVKGSRFMR
                     MERVVDALSAPVGGEAEGARAA"
     gene            complement(7017..8522)
                     /locus_tag="PKV30_02920"
     CDS             complement(7017..8522)
                     /locus_tag="PKV30_02920"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRL29205.1"
                     /translation="MTTTALHTPKEAARWLREHVRGQLQADSRRVGPGDGFIAWPGAA
                     TDGRRYVGAALAQGASACLVEQAGVEAFGFDQPEVATYAHLKAATGHIAAAYYEAPSE
                     ALNIIAITGTNGKTSTAWWLAWALSKAELPALAPCALVGTLGMGSPSDLQATGMTTPD
                     PVLLQQRFRAFVNAGVKSCAIEASSIGIVERRLDGTAIRVAVFTNFTQDHLDYHGSMQ
                     AYWQAKAELFDCPGLQAAVINIDDPRGAELVQRAEARGLDVWTVSCQGPARLRATDIG
                     YGAHGLDWVLREGERAESLRTALVGEYNVANLMCVLGALRALGLDLAQAVAACTDLPP
                     VPGRMERIDLAGAPLAVIDYAHTPDAVDKALQALRPLSQKRAGRLWCVLGCGGNRDAA
                     KRPLMAAAAERGADRVVLTSDNPRDEDPLAIIAAMRAGLHHEGAVAVQPDRAEAIALA
                     LAQAAPEDVVLIAGKGHEDYQEVRGQRRAFLDQAEARRALAQRGTAAGQAA"
     gene            complement(8524..10260)
                     /locus_tag="PKV30_02925"
     CDS             complement(8524..10260)
                     /locus_tag="PKV30_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRL29206.1"
                     /translation="MRDIRYTSSPLLASKTPVWRSKFIVAMLALAFIVLFARAAYVQV
                     IGNAFFQRQGEVRFARTLEMPASRGRIFDRNGLLLASSVVAPSIWAIPDDVERNPAKL
                     QQLAKLLGMPMAELNKKLADEDKTFVWLKRQVDEPVAKQIAELKIAGIHQRKEYKRKY
                     PEGEAAAHVVGFANVENRGQEGAELSFEKQLSGKAGARRVIKDRLGRVVEAVGDEVPP
                     VDGQDVQLSIDSKVQFFAYQKLRDAVTAHKAKSGSVVVLDAQTGEVLALANYPSYSPD
                     NRRNLTGAQLRNIAITDTFEPGSTMKPITVAMALEAGKVTPQTQIATAPGTYKLDKFT
                     IRDTHNYGTLTVEGVVQKSSNVGSLKIAQRLSAQEMWDTYTALGYGHKPDLAFPGAAT
                     GRVRHWKGWRPVEQATMSYGYGLSASLFQMAHSYTAFSHDGRVIRATLVKAPDDTAVE
                     GVRVFSEKNARAVRKMLEMAAGPGGTGQRAQTVGYSVGGKSGTARKQQGKGYASNKYR
                     AWFTGMAPIDKPRVIVAVMVDEPSNGVYYGGAVAAPVFSEVVQQTLRIMGVQPDMAVK
                     PAIVAEQVEESF"
     gene            complement(10257..10562)
                     /gene="ftsL"
                     /locus_tag="PKV30_02930"
     CDS             complement(10257..10562)
                     /gene="ftsL"
                     /locus_tag="PKV30_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRL29207.1"
                     /translation="MTRLSIVLLAAVMFSALYLVRVQYDSRRLFTELDRATAEARRLD
                     VEHSRLEVEKRAEATSLRVEKLAKDKLAMRTVTPAITEYVTSSVALPVASASQGVRP"
     gene            complement(10559..11473)
                     /gene="rsmH"
                     /locus_tag="PKV30_02935"
     CDS             complement(10559..11473)
                     /gene="rsmH"
                     /locus_tag="PKV30_02935"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRL29208.1"
                     /translation="MNTWSHTTVLLNEAVDALVTDPQGQYVDATFGRGGHARLILQRL
                     GAGGQLVAFDKDPDALAEAARIEDARFSIRHQGFAALGELPPGGAAGVLMDLGISSPQ
                     IDNPARGFSFRADGPLDMRMDPTRGQSAADWLAVADQRQIAEVIRDYGEERFAGPIAK
                     AIVSRRQERGPVATTLELAELVAGAVKTREPGQNPATRTFQAVRIFVNAELEELEQAL
                     KGCLHVLQPGGRLVVISFHSLEDRIVKQFIARHSKAVVDRRAPFAEPPPTLLRALGRV
                     RASEAEVAANPRSRSAVMRVAERTEAPA"
     gene            complement(11488..11916)
                     /gene="mraZ"
                     /locus_tag="PKV30_02940"
     CDS             complement(11488..11916)
                     /gene="mraZ"
                     /locus_tag="PKV30_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRL29209.1"
                     /translation="MFQGASSLSLDAKGRLSVPTRHRDVLSATAAGQLTITKHPHGCL
                     MVFPRPEWEKFRERIAQLPMSAQWLKRIFLGSAMDVDMDGTGRVLVSPELRAAAGLTR
                     DAMLLGMGSHFELWDKTTYDAKEAEAIQAGMTQAFEELVF"
     gene            complement(12302..13630)
                     /gene="hslU"
                     /locus_tag="PKV30_02945"
     CDS             complement(12302..13630)
                     /gene="hslU"
                     /locus_tag="PKV30_02945"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRL29210.1"
                     /translation="MSSMTPQEIVSELDRHIVGQQDAKRAVAIALRNRWRRQQVDEKL
                     RPEITPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGKDVDSIIRD
                     LAEMAVKQQREQAMKAHRTRAEDLAEDRVLDVLIPPARNADGSAAPSDGAARQNFRKK
                     LREGQLDDKEIELELAQPAPTMEIMGPAGMEDMAEQLKGMLGSLSAGRRKLRRVKIGE
                     AMKLLIDEEAGKLVNEDDIRTEALKNLEQNGIVFIDEIDKVASRGEGGNTADVSRQGV
                     QRDLLPLVEGTTVSTKYGMVRTEHILFIASGAFHLSKPSDLIPELQGRLPIRVELASL
                     TVQDFEAILTQTDASLVRQYQALLATEGVTLAFTAEGISRLAEIACAVNESTENIGAR
                     RLSTVMERLLDEVSFDATRLEGQTVTIDAPFVDQRLAALSRDEDLSRYIL"
BASE COUNT         2260 a         4691 c         4585 g         2241 t
ORIGIN      
        1 tctccatgtg gtcggcgtcg atgttggtga cgacggccat cacaggcagc aggttcaaaa
       61 aggaagtatc ggactcgtcc gcctcgacca cgatgtagtc gcccgagccg agccgcgcgt
      121 tggcgcctgc gctgttcaat cgcccgccga tgacgaaggt cgggtccagc cccgcttcgg
      181 ccagcaggct ggcgaccagg ctggtcgtgg tggtcttgcc gtgcgtgccg gcgatggcga
      241 tgccctgctt gaggcgcatc agctcggcca gcatcatggc gcgcggcacc acgggaatgc
      301 gcttgtcgcg cgcgaccagc acctcggggt tgtcgggctt gacggcggtg gaggtgacca
      361 cggcgtccgc gccctcgacg ttggccgccg catgaccaac aaaggtgcgg atgcccatgc
      421 cagccaaccg gcgcagcgtg gcgctgtcgg ccaggtcgga gccggagatg acgtagccca
      481 ggttgtgcag cacctcggcg atgccgctca tgccggagcc accgacgccg acgaaatgga
      541 tgtggcggat ggcgtgtttc atcgcgcggt caactcctcg cacgccgtca cgacgtcttc
      601 gacggcatga atcttttgca tctttttagc cgccagcgcc cgttggacaa gcgcggaacg
      661 ctctgttttt tgaagcattt ccgccaagcg ctcgggcgtc aggtcgcgct gctgaacgag
      721 ccagccggcg ccctgatcga ccaggaactt cgcgttggtg gtctggtgat cgtccaccgc
      781 gtgcggaaag ggcacgaaca gcgcggcggc gccgatggcg gcgatctcgg tcaccgtggt
      841 ggcgccggcg cggcagatca ccaggtcggc gtcggcatag gacttggcca tgtcgtcgat
      901 gaacggcacc agttcggctt gcacgccggc cgcgtcgtag tttgcgcgca gcgcgtcgat
      961 ctgcttggcg ccgctctggt gcaccacctg cgggcgctgt ccggcgggga tcagcgccag
     1021 cgccttcggc accacgtcgt tcagcgcctg cgcacccagg ctgccgccga cgacaagaat
     1081 cttgagcggg ccgctgcgac aggcaaagcg catggccgga tcgggcacct gcttgaacga
     1141 ctcgcgcagc gggttgccca cccattgcac cttgcgcgcc ttgggcagca cgccggggaa
     1201 cgccgtgaac acgcggtcgg cgatgtgcgc cagcaccttg ttggccatgc cggcaatcga
     1261 gttctgctcg tgcagcacca gcggcttgtt cagcgccacg cccatcagcc cgccaggaaa
     1321 ggtgatgtag ccgccaaagg cgaccagcac gtcgggcttg acgcggcgga tcacgccgat
     1381 gctctgcgcg aaggctttca gcaaacgcaa cggcagcatg aacaacgtgg tcacgccctt
     1441 gccgcgcacg ccgccgaatt cgaccggctc gaaggcgtag ccgcgcggcg gcaccagttg
     1501 cgcctccatg ctgccgggca cgcccagcca gtgcacgcgc cagccgcgcg cgcgcaagcc
     1561 atcggccacg gcaagcccag gaaagatgtg gccgcccgtg ccgccggcca tgaccagcgc
     1621 gcacttctgc gtcacgtgcg ccccccgtgc atcaaacggc ggttctccac atctacgcgc
     1681 agcacgatgg caatggccac catgttggcc aggatggccg agccgccata gctcatgaag
     1741 ggcagcgtca gccccttggt gggcagcgcc cccaggttga cgccgatgtt gatgaaggcc
     1801 tgaaagccca gccacagccc gatgccctgc gccaccagcc ccgagaacac gcggtccagc
     1861 gcgatcgcct gacggccgat gtgcatgatg cggcgcgtca gccagaagaa cagcacgatc
     1921 acgcacagca ggccgaccaa gccaaactct tcgccgatca cggccagcag gaagtcggtg
     1981 tgtgcctcgg gcagccagtg cagtttctcg atgctgccgc cgaggccaac gccaaacacc
     2041 tcgccgcggc cgatggctat cagcgcgtgc gacaactggt agcccttgcc cagcgcatgg
     2101 tcctcgctga acggatcgag gtaggcaaag atgcgctcgc gccgccaggg cgaggcccag
     2161 atcatcaacg caaacgcgac caccagaaac gccgcgatga tgaagaacat gcgcgcgttg
     2221 acgccaccca gaaacagaat gcccatggcg atgacggcga tcaccatgaa ggcgcccatg
     2281 tcgggctgcg ccagcaacag cgcgcccacc acgccaaccg ccacgcccat cggcagcacg
     2341 gcgcggaaga agcgctcctt cacctccatc ttgcgcacca tgtagtcggc ggcgtagagc
     2401 agcacggtga tcttggccac ttcggagggc tgaaagctca tggggccgag cgcgatccag
     2461 cgccgcgcgc cgttgacgac gctgcccacg tgcggaatca gcaccgcgat cagcagcgcc
     2521 agcgagacga cgaacagcca cggcgccgcc ttctcccacc agtccatgcg cacctggaag
     2581 gcgatcaacg cggcgacaaa cgcgatgccg atccacatcg cgtgcctgaa gacgaagtag
     2641 ttgggcgagt agcgtccaaa acgcgggttg tcgggcagcg cgatgctggc cgaatacacc
     2701 atcaccagac cccaggccag cagcgcgacg caggcccaaa tgagcgcctg gtcgaagccc
     2761 agcacgcgcg ccggcgtgct ggccgtgcgc gcgtactcca tgccgccgat gcgcaccggc
     2821 agttcgtcgg ccaggcgctt cttggcgcgc gcggcgcgcg gcttctttct gctgggtggg
     2881 ttcagcgcgt cggcggcggc gttcatccga gcacctccag gtcggttccg gcggccagcg
     2941 ccagatcgcg cacggcggcg cggaacactt cggcgcgctc gccgtagttc ttgaacatgt
     3001 cgaggctggc gcaggcgggc gacagcagca cggcgtcgcc gctgtgggcg cgcgcctgcg
     3061 cctgcgcgac ggcgtcgggc atgtcggtag cgtccagcag cggcacgccg gtgtctcgca
     3121 gcgcggcgcg cagcagcggc gcatcgcggc cgatcagcac cacggcgcgc acgaagcgcg
     3181 ccaccggtgc ggccagcggc gaaaaatcct gccccttgcc atcgccaccc aggatcacga
     3241 ccaatttgcg ttcggtgccc aggccctcca gcgcggccac ggtggcgccc acgttggtgc
     3301 ccttgctgtc gtcgaagtac tcgacgccat ccagcaggcc aatggattcc acgcggtgcg
     3361 gctcgccgcg gtattcgcgc aggccgtaca gcatggggcc gagcgcgcag ccggcactgg
     3421 tggccagcgc cagcgcggcc agcgcattga cggcgttgtg gcggccgcga atgcgcagcg
     3481 cgtcggccgg catcaggcgc tggatgtgca actcgacctc ggcgtccttg cggcggcgct
     3541 gcgtctcgtc ggcctccagc gcgcgcacca gccaggccat gccacccacc agttcaatgc
     3601 cgaagtcgcc gggttcggtg ggcatgtcgg cgccgaaggt cagcacggtg cgcggctcgg
     3661 gcttcggcgt gcccttgggc actttggcgg gcggtgcggg gcgcatggcc atgacggccg
     3721 cgtcgtcgcg gttcagcacc atcacgccgt gctcgccaaa gatgcgcgcc ttggccgctg
     3781 cataggccgc catgtcgccg tgccagtcca ggtggtcctg cgtgatgttg agcacggtgg
     3841 cggcggtggg ctcgaagccc gccacaccat cgagctggaa gcttgacagc tccagcaccc
     3901 acacttcggg cagcgtctcg gccaccagat gcgcctgcaa cgtgtccagc agcgtcggac
     3961 cgatgttgcc agccaacacc accgagcgac ccgcgcgctc gaccagctgg ccggtcagcg
     4021 ccgtcaccgt ggtcttgccg ttggtgcccg taatggccag cagcgcaggt tcgtagccga
     4081 ttgtgtgctt caaattcgag agcgtctggg tgaacagatc cagctcgccg cccaccagca
     4141 acccacgctc gcgtgccgcc tgcaccacgc ccgcgatgct gatcggtgcc aggccggggc
     4201 tgcggtacac ggcgtgcagg ctggcatcgt caagcagcga ggcgtccatg gcgccatgca
     4261 cgaagcgcgc cgccggcacc tgctggcgca gcgcatcgag ttgtggcggc gcctcgcgcg
     4321 tgtccaccac cgtgacttgc gcgccctgcc ctgcgcacca gcgcgccatg gccaggcccg
     4381 acaagcccag gccgaggacc aacacgtgac ggtcttgcag catcgtcatc gcatcaccgc
     4441 agtttcaacg aagacaaacc caccaggcac agcagcatgg tgatgatcca gaagcgcacc
     4501 acgacctgcg tttcggccca gccgcttttc tcgaagtggt ggtgcagcgg cgccatcttg
     4561 agcaggcgcc ggccctggcc gaaacggcgc ttggtgtact tgaagtagct cacttgcagc
     4621 atcaccgaca gcgcctcgac cacgaagatg ccgcccatga tggccagcac gatttcctgg
     4681 cgcacgatga ccgctatcgt tcccagagcg ccgccgagcg ccagcgcgcc gacgtcgccc
     4741 atgaacacct gcgcggggtg ggcgttgaac cacaggaagg ccaaacccgc gcccgccatg
     4801 gcggcgcaga agatcagcag ctcgccagtg cccggaatgt gcggaaacag caggtacttg
     4861 gaaaaaaccg agctgcccgt aacgtaggca aaaatgccca gcgccgagcc gaccatgatg
     4921 accggcatga tggccagccc gtccagcccg tcggtcaaat tgacggcatt gctggagccg
     4981 acgatgacca gatagctcag gatcacaaag ccagccacac ccagcgggta gctcacttcc
     5041 ttgaagaacg gcaccagcag accagcagcg tgcggcaggt caatggtgaa gccggacgac
     5101 acccagccga cgaacagcgc ccacacctcg acgttggacg cgcccgagat ggcaaacgcc
     5161 aggtacagcg ccgccagcag gccgatgacc gattgccaga aatacttctc gcgcgagcgc
     5221 atgccctcgg ggtccttgtt gaccaccttg cgccagtcgt cggcccagcc gatggcgcca
     5281 aagccgaagg tgacgatcat cacgatccag acgaagcggt tggaccagtc gaaccacagc
     5341 aaggtcgaga tggcgatcga caacaggatc aacacgccgc ccatggtggg cgtgccgctc
     5401 ttgctgaggt gcgtttccat cgcgtagccg cgcaccggct ggccgatctt cagctcggtc
     5461 agcttgcgga tcacccaggg gccggccacg atgccgatga ccagcgccgt catggcggcc
     5521 atcacggcgc ggaacgtgag gtattgaaag acgcggaaga acccgaactc gggcgacagc
     5581 gcctgcagcc aggtggccag actaagcagc acgggcaccc tccgcttcgc cgcccacggg
     5641 cgcggacagg gcatctacca cgcgctccat gcgcatgaag cgcgagcctt tcacggcaat
     5701 cgacgtgcac tgcggcaggt gcgtgcgcac ggcgtcggtc aaggccgcca tgtcgtcgaa
     5761 atgccggccg ccgccgaagg ccatggcggc gtggatcgcc tgcgggccgt gcgccagcag
     5821 gcgctcgatg ccgcgctcgc gcgcgtaggc gccgacttcg gcatggaagg ccgggccctg
     5881 gtcgccgacc tcgcccatgt cgcccagcac cagcaggcgc ggaccgggca gttcggccaa
     5941 cacgtcgatg acggcgcgca ccgaatcagg gttggcgttg taggtgtcgt ccaccagcgt
     6001 gatcgcgcgg ccgcgatgcc gcagcagcag cgtgcgcgag cggcccttga cggggcgaaa
     6061 ggcgctcaag ccctgcgcga tggcggccag cggcgcgcca gcggccgtgg tgcaggcgat
     6121 ggcggccagt gcgttgcgca ggttgtggcg gccggccgcg tgcagctcga aagccacgtc
     6181 gcccgccggt gtgcgggcag tggcttgcca gtgctcggcg cgccaggcgg cgttgatcaa
     6241 agacacttcg gcaccgtcag actgctctcc aaacaatagc tgcctgcgct tgccagacaa
     6301 gcgcttccac gtgttttgat acaaatctcc ggcgggaaac accgcagtgc cgccgtcgcc
     6361 cagcgcggta aacaccgagc cgttttcctc agccacggcc tgcacgctcg ccatgaactc
     6421 ctggtgctcg cgctgcgcgt tgttgaccag cgccaccgtg ggctgggcga tggcggccag
     6481 ctgcgcgatc tcgcccgggt ggttcatgcc cagctccacg accgccacct tgtgctcggg
     6541 ccgcaggcgc agcagcgtca gcggcacgcc gatgtcgttg ttgaagttgc cctgcgtggc
     6601 cagcgcggca tcgccctgcc aggcgcacag gatggacgcg atcatctgcg tcaccgtggt
     6661 cttgccgttg ctgccggtga cggcgatcag cgggccatcg aaacggcggc gccaggcgtg
     6721 cgccagctcg cccaaggcgc ggcgcgcatc cggcaccagc acgcccggca ggccggatgc
     6781 ccgcagcgac tcttcggcag acgcatcgca cagcgcggcc accgcgcctt gcttggccgc
     6841 gtcgggcaga aaggcggcgg cgtcaaagcg ctcgcccttc agcgcgacga acaaatcgcc
     6901 cggccgcagc gtgcgcgtgt cggtgtgcac gcggtcgcag cgcacgcccg cgtcgccgat
     6961 caggcgcgcg ccagccagcc agcccgcgag ttcgcccagc gtcaggttca tggcgttcat
     7021 gccgcctgcc ccgccgctgt gccgcgctgc gccagcgcgc ggcgtgcctc ggcttgatcc
     7081 aagaaggcgc gccgctggcc gcgcacttcc tggtagtcct cgtggccctt gccggcgatc
     7141 agcaccacgt cctcgggtgc cgcctgcgca agcgccagcg cgatggcctc ggcgcgatcg
     7201 ggttgcaccg ccacggcgcc ttcatgatgc agcccggcgc gcatggcggc gatgatggcc
     7261 agcggatctt cgtcgcgcgg gttgtcgctg gtcagcacca cgcggtcggc gccgcgctcg
     7321 gcggcagcgg ccatcagcgg gcgcttggcg gcgtcgcggt tgccgccgca gcccagcacg
     7381 caccacaggc ggccagcgcg cttttggctc agcgggcgca aggcctgcag tgccttgtcc
     7441 accgcgtcgg gcgtgtgggc atagtcgatc acggccagcg gggcgccggc caggtcgatg
     7501 cgttccatgc ggcccggcac gggcggcaga tcggtgcagg cggcgacggc ttgcgccaga
     7561 tccaggccca gcgcgcgcag tgcgcccagc acgcacatca ggttggcgac gttgtactcg
     7621 cccaccaggg cggtgcgcag gctttcggcg cgctcgcctt cgcgcagcac ccaatccagc
     7681 ccgtgcgcgc cgtagccgat gtccgtggcc cgcaggcgtg ccgggccctg gcacgagacg
     7741 gtccagacgt cgagcccgcg cgcttcggcg cgctgcacca gctcggcccc gcgcgggtcg
     7801 tcgatgttga tgactgccgc ttgcagcccc gggcagtcga acagctcggc cttggcttgc
     7861 caataggctt gcatgctgcc gtggtagtcg aggtggtctt gcgtgaagtt ggtgaacacg
     7921 gcgacgcgga tcgccgtgcc gtccaggcgc cgctcgacga tgccgatgga cgatgcctcg
     7981 atggcgcagc ttttgacacc ggcatttacg aacgcgcgaa agcgctgctg cagcagcacc
     8041 gggtcgggcg tggtcatgcc ggtggcttgc agatcggacg gcgatcccat gcccaaagtg
     8101 cccaccagcg cacacggggc aagcgcgggc agctctgctt tcgatagtgc ccaggccagc
     8161 caccaggcgg tcgatgtctt gccgttggtg ccggtgatgg cgatgatgtt gagcgcctct
     8221 gacggcgctt cgtagtacgc ggcggcgatg tggccggtgg cagccttcag atgtgcgtag
     8281 gtggcgactt cgggttggtc gaagccgaac gcctcgaccc ccgcctgctc gaccaggcac
     8341 gcgctcgcgc cttgcgccag cgcggcgccg acgtagcggc gaccatcggt ggcggcgccc
     8401 ggccaggcga tgaagccatc gcccgggccg acacgccggc tgtcggcctg cagctggccg
     8461 cgcacgtgct cgcgcagcca gcgcgcggct tccttgggtg tgtgaagcgc ggtggtcgtc
     8521 atctcagaaa gactcttcca cctgctcagc cacgatggcc ggcttgacgg ccatgtcagg
     8581 ctgcacgccc atgatgcgca acgtctgctg caccacttcg ctgaacaccg gcgccgccac
     8641 ggcaccgccg tagtacacgc cgttgctggg ctcgtcgacc atcacggcga cgatgacgcg
     8701 cggcttgtcg atcggcgcca tgccggtgaa ccaggcgcgg tatttgttgc tggcgtagcc
     8761 cttgccttgc tgcttgcggg cggtgccgga tttgccgccg accgagtagc ccacggtttg
     8821 cgcacgctgg ccggtgccgc cagggccggc ggccatctcc agcatcttgc gcacggcacg
     8881 ggcgttcttc tcggagaaca cgcgcacgcc ctccaccgcc gtgtcgtctg gcgccttgac
     8941 cagggtggcg cggatcacgc ggccgtcgtg cgagaacgcg gtgtacgaat gcgccatctg
     9001 gaacagagag gccgacaggc cgtagccgta ggacatggtg gcctgctcga ccgggcgcca
     9061 gcccttccag tgccgcacac ggcccgtcgc ggcgccggga aaggccaggt ccggcttgtg
     9121 gccgtaaccc agcgccgtgt aggtgtccca catctcctgt gccgacaggc gctgcgcgat
     9181 cttcagcgag ccgacgttgc ttgacttctg caccacgccc tcgacggtga gcgtgccgta
     9241 gttgtgcgtg tcgcggatgg tgaacttgtc gagcttgtag gtgccgggcg cggtggcgat
     9301 ctgcgtctgc ggcgtcacct tgccggcctc cagcgccatg gcgacggtga tcggcttcat
     9361 ggtcgagccg ggctcgaagg tgtcggtgat ggcgatgttg cgcagttgcg cgccggtcag
     9421 gttcctgcgg ttgtcgggcg agtagctggg gtagttggcc agcgccagca cttcgcccgt
     9481 ctgcgcgtcg agcaccacca cgctgcccga cttggccttg tgcgcggtga cggcgtcgcg
     9541 cagcttctgg taggcaaaga actgcacctt ggaatcgatg gacagctgca cgtcctgacc
     9601 atccacaggc ggcacctcgt caccgaccgc ctcgaccacg cggcccaggc ggtccttgat
     9661 gacgcggcgc gcgccggcct tgccggacag ctgcttctcg aacgacagct cggcgccttc
     9721 ctggccgcgg ttctcgacgt tggcaaagcc gaccacgtgc gcggcggcct cgccttcggg
     9781 gtacttgcgc ttgtattcct tgcgctggtg gatgccggcg atcttcaact cggcaatctg
     9841 cttggccacc ggctcgtcga cctggcgctt gagccagacg aaggtcttgt cttcgtcggc
     9901 gagcttcttg ttcagctcgg ccatcggcat gccgagcagc ttggccagtt gctgcagctt
     9961 ggcgggattg cgctcgacgt catcaggaat cgcccagatg ctgggcgcaa ccacgctgga
    10021 cgccagcagc aagccattgc ggtcgaagat gcggccgcgg ctggccggca tttcgagcgt
    10081 gcgggcaaag cgcacttcgc cctggcgctg gaagaaggca ttgccgatca cctgcacgta
    10141 ggccgcgcgc gcaaacagca cgatgaaggc cagcgccagc atggcgacga tgaacttgct
    10201 gcgccacacc ggcgtcttgg acgccagcaa cgggctggag gtgtagcgga tgtccctcat
    10261 ggccgcaccc cctggctggc cgaggccacg ggcagcgcca ccgacgaagt cacgtactcg
    10321 gtgatggccg gcgtcacggt acgcatcgcg agcttgtcct tggccagctt ctcgacgcgc
    10381 agcgaagtgg cttcggcgcg cttttccact tcaagccggc tgtgctcgac gtcgagccgg
    10441 cgcgcctcgg cggtggcgcg gtcgagttcg gtgaacaggc gacgcgagtc gtactgcacg
    10501 cgcaccaggt acagcgcgga gaacatgacg gcggccagca gaacgatgga cagcctcgtc
    10561 atgccggcgc ctccgtccgc tcggccaccc gcatgacggc gctgcgcgaa cgcgggttgg
    10621 ccgccacttc ggcttcgctg gcgcgcacgc ggccgagcgc acgcagcaag gtcggcggcg
    10681 gctcggcaaa cggcgcgcgg cggtccacca ccgccttcga atggcgggcg atgaactgct
    10741 tgacgatgcg gtcttccagc gaatggaagc tgatcaccac gagccgacct ccgggttgca
    10801 gcacatgcag gcagcctttc agcgcctgtt cgagctcttc aagctcggcg ttgacgaaaa
    10861 tccgaacagc ctgaaatgtg cgcgttgcag ggttctggcc cggctcgcgg gttttgaccg
    10921 cgccagccac gagttcggcc agttcaaggg tggttgcaac agggccccgt tcctgtcggc
    10981 gcgacacaat cgcctttgca atggggccag caaaccgttc ttcgccgtag tcacgaatca
    11041 cctccgcgat ctgccgctga tcggccaccg ccagccagtc ggccgcgctc tgaccgcgcg
    11101 tggggtccat gcgcatgtcg agcgggccgt cggcacgaaa agaaaaaccc cgcgcggggt
    11161 tgtcgatctg tggcgagctg atgcccaaat ccatcaacac cccagcggcc ccgccaggcg
    11221 gcaattcgcc cagcgccgca aagccctggt gccggatgga aaagcgcgca tcctcgatgc
    11281 gcgctgcctc ggccagcgcg tccgggtcct tgtcgaacgc caccagttgg ccgcccgcgc
    11341 ccagccgttg caggatcagc cgcgcatgac cgccgcgccc gaaggtcgcg tcgacgtact
    11401 ggccttgcgg atccgtgacg agcgcatcga ccgcctcgtt caacaagacc gtggtgtgtg
    11461 accatgtgtt cacccgccat cgccccctca gaacacgagt tcctcgaacg cctgcgtcat
    11521 gccggcctgg atggcttcgg cttccttggc gtcgtaggtg gtcttgtccc acagttcgaa
    11581 atggctgccc atgcccagca gcatggcgtc gcgcgtcagg ccggcggcgg cgcgcagctc
    11641 gggcgacacc agcacgcggc cggtaccgtc catgtcgacg tccatggcgc tgcccagaaa
    11701 gatgcgcttg agccactgcg ccgacatggg cagctgcgcg atgcgttcgc gaaatttttc
    11761 ccactcggga cgcggaaaca ccatcagaca gccgtgcggg tgcttggtga tggtgagctg
    11821 gccggcggcg gtcgcgctca ggacgtcacg atgccgggtc ggcacggaaa gcctcccctt
    11881 tgcatccaga ctcagcgatg acgccccttg aaacacgtga tttccctgct tgaagtgccg
    11941 cagcgcacgg gagacggcac tttcccccac ttaattgcac ttttttgcac tgtagcagga
    12001 aaccccagca ggacaagggc ggacaacaaa ttatttcaat gaaatcaacg ctttagctgg
    12061 gttttccatc agatgtctgg ggcaaaattt ctttgaaatt aggcacttag cgtcgacagt
    12121 caaagtacgg tcattgaagc ggggtgggta tcgagcggcc acacggcggc acgggcagcc
    12181 gcttgcgctg cggcgcgcgg gcatgaccga tgggtgcggc attcacacga atgccatcat
    12241 tttagctatt gattgaatag ctgctcgcgc ttgtccggca agcactgaag cctgatttgg
    12301 cttaaagaat gtagcgcgac aggtcttcgt cgcggctcag cgccgccagg cgctgatcga
    12361 cgaaaggcgc gtcaatggtc acggtttggc cttcgagccg ggtggcgtcg aagctgacct
    12421 cgtccagcag ccgctccatc acggtcgaga ggcgccgcgc gccgatgttc tcggtcgact
    12481 cgttcaccgc gcaggcgatc tcggccagcc gcgagatgcc ttcggccgtg aaggccagcg
    12541 tcacgccctc ggtggccagc agcgcctggt actggcgcac cagcgatgca tcggtctgcg
    12601 tcaggatggc ctcgaagtcc tgcaccgtga gcgacgccaa ctcgacgcga atcggcagcc
    12661 gcccctgcaa ctcgggaatc aggtcgcttg gcttggacag gtggaaggcg cccgaggcga
    12721 tgaacaggat gtgttcggtg cgcaccatgc cgtatttggt gctcacggtg gtgccttcga
    12781 ccagcggcag caaatcgcgc tgcacgccct ggcgcgacac gtcggccgtg ttgccaccct
    12841 cgccgcgcga agccaccttg tcgatctcgt cgatgaagac gatgccgttc tgctccaggt
    12901 tcttcagcgc ctcggtgcgg atgtcgtcct cgttcaccag cttgccggcc tcttcgtcga
    12961 tcagcagctt catcgcttcg ccgatcttca cgcgccgcag cttgcggcgg ccggcgctca
    13021 aggaacccag catgcccttg agttgctcgg ccatgtcttc catgcccgcc ggacccatga
    13081 tttccatggt gggcgcgggt tgcgcgagtt cgagttcgat ttccttgtca tccagttgcc
    13141 cctcgcgcag cttcttgcgg aagttctggc gcgcggcgcc gtcactgggc gctgcgctgc
    13201 cgtcggcgtt gcgggcgggc gggatcagca cgtcgagcac gcggtcttcc gccaggtctt
    13261 cggcgcgcgt gcggtgcgcc ttcatggcct gctcgcgctg ctgcttgacg gccatctcgg
    13321 ccaggtcgcg gatgatggaa tcgacgtctt tgccgacgta gcccacctcg gtgaacttgg
    13381 tcgcctcgac cttgatgaaa ggcgcgttgg cgaggcgcgc cagacgccgc gcaatctcgg
    13441 tcttgccgac gccggtgggg ccgatcatca ggatgttctt gggcgtgatc tcgggccgca
    13501 gcttttcatc gacctgctgg cgccgccagc ggttgcgcag cgcaatggcg acggcgcgct
    13561 tggcgtcctg ctggccgacg atgtggcggt cgagttcgga gacgatctcc tgcggggtca
    13621 tgctggacat ggcggactat ttataaaaat cgggctgcag agcttgccaa tcaagcgcgg
    13681 ccagctatca atttgatggc agttggatca gagcgattca acggtgtggt gcatgttggt
    13741 gtagatgcac agctcgccgg cgatctccag cgccttc
//
LOCUS       DAOMVO010000030        13629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1000_length_13629_cov_15.385001, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000030 DAOMVO010000000
VERSION     DAOMVO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_13629_cov_15.385001"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PKV30_02950"
     CDS             <1..1058
                     /locus_tag="PKV30_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRL29211.1"
                     /translation="EYPSRNPQQNMADLKAQIAANEKGVQELRKMVDQFGLDVVQAYM
                     KHVQDNAEESVRRAITKLAARMQGGQFTLPLDNGAQIQVAVTIDGAARSAKVDFTGTS
                     PQQINNFNAPTAVCMAAVLYVFRMLVDDDIPLNAGCLKPIEVVIPEGSMLRPNPPASV
                     VAGNVETSTCITNAIIGALGLMGASQCTVNNFTFGNDKYQYYETISGGSGAGGVFDDA
                     GQLVGGFPGTSVVQTHMTNSRMTDPEVLEWRFPVRLESYEIRSDSGGKGQWNGGHGGV
                     RRLRFLEPMTASILSNGRLRGAFGMAGGGAGAVGINRVVRASGTVQDLQHIGSAEMQP
                     GDIFEIHTPGGGGFGAV"
     gene            complement(1208..1672)
                     /locus_tag="PKV30_02955"
     CDS             complement(1208..1672)
                     /locus_tag="PKV30_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRL29212.1"
                     /translation="MFKKLLVLLSLFYAALSFAAVDVNTGSAADLDSVKGIGPGMSKR
                     IINERAKGQFKDWGDFMARVKGVGEKNAANFSAGGMTVNGAGYAGAPAKPAKDAKAKK
                     EKTVAAAPAAGATPAAAGTTAAIPAIATKPAAPAMPAAPAAPAKPAAPAVKQ"
     gene            complement(1773..2933)
                     /gene="lapB"
                     /locus_tag="PKV30_02960"
     CDS             complement(1773..2933)
                     /gene="lapB"
                     /locus_tag="PKV30_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HRL29213.1"
                     /translation="MEFDLSWILLGVPLVFALGWLASRLDLRQLRIENQRAPKAYFKG
                     LNYLLNEQQDQAIDAFIEAVQKDPDTSELHFALGNLFRRRGEYERAVRVHEHLLSRAD
                     LSQSDRDRAQHALAQDFLKAGLLDRAEDALRKLEGTRYQEQAWLTLLSLHERARDWPQ
                     AATVAGRLQASGQADFSARQAHYLCEQAAEALNKKHDAQAAQQLLLQAIEQTPSAARP
                     RIQLAGLLAGRDDWSGAFEQLISLEHHAPQAVPLVASELVRIGHASGQLAQARQLLER
                     SYAATPSVDVADALVQAEVTAGTPLHDARAGYVRHMAQEPSLIAAARWLSQEAFAQEG
                     AHPVVQRSVEHAARPLARYRCAACGFEAKGYFWQCPGCQAWESFPPRRIEEL"
     gene            complement(2923..3222)
                     /locus_tag="PKV30_02965"
     CDS             complement(2923..3222)
                     /locus_tag="PKV30_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRL29214.1"
                     /translation="MTKLTRLLTWILKAAVFFALFAFALNNQQDATVHLLFGHQWRAP
                     MMLIVLAAFALGLVVGVLGMLPGWRSRRNVRGHAEPARSDLAPLDDQLTSPPHGV"
     gene            complement(3267..3575)
                     /locus_tag="PKV30_02970"
     CDS             complement(3267..3575)
                     /locus_tag="PKV30_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRL29215.1"
                     /translation="MTRSDLVELLAERFGQLTQRDADAAVKTILDAMSDALVRGHRIE
                     IRGFGSFSINRRPPRLGRNPRTGESVHIPERRVPHFKPGKALREAVGAQPVDAGDAGP
                     "
     gene            complement(3707..5386)
                     /gene="rpsA"
                     /locus_tag="PKV30_02975"
     CDS             complement(3707..5386)
                     /gene="rpsA"
                     /locus_tag="PKV30_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRL29216.1"
                     /translation="MSESFAQLFEESLQRTEMRPGEVITAEVVRVEHSFVVVNAGLKS
                     EAYIPIEEFKNDQGEVEVEPGAFVPVAIGSIENGYGDTILSRDTAKRLASWLALEKAL
                     ESGDFVTGTTSGKVKGGLTVLVNGIRAFLPGSLVDTRPTKDLTPYENKTLEFKVIKLD
                     RKRNNVVLSRRAVVEASMGEERAKLMETLKEGAVVNGVVKNITEYGAFVDLGGIDGLL
                     HITDMAWRRVRHPSEVVQAGQEITAKILKFDTEKNRVSLGLKQMGDDPWLGVSRRYPQ
                     GTRMFGKVTNIADYGAFVELEPGIEGLVHVSEMDWTNKNVAPSKIVSLGDEVEVMVLE
                     IDEDKRRISLGMKQCRANPWQEFAQNTKRGDRVKGPIKSITDFGVFVGLAAGIDGLVH
                     LSDLSWNETGEAAVRNYKKGQEVEAIVLAVDVDRERISLGIKQLDQDPFTTFVTVNDK
                     GQIVSGTVKTVDARGAEIDLGQDVIGYLRASEISRDRVEDASQVLKVGDEVTAVVVNV
                     DRKTRNIQLSVKAKDAADQQEAMASLNQNTGTAGTTSLGALLRAKLDNNDK"
     gene            complement(5594..7591)
                     /locus_tag="PKV30_02980"
     CDS             complement(5594..7591)
                     /locus_tag="PKV30_02980"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HRL29217.1"
                     /translation="MFETEFLDVPALRVVSGTVRLPGSKSISNRVLLLAALCEGQTTV
                     RDLLDSDDTRVMLQALRQLGCGVAVDGTTAVIDGLGGRARQPQAELFLGNAGTAMRPL
                     TAALAVLGGDYALSGIPRMHERPIGDLVDALRALGCNVQYLGQDGFPPLHIGQPNLRL
                     DQPIPVRGDVSSQFLTALLMALPLAAQAQDIVIDVVGELISKPYIEITLQLLARFGVK
                     VQRNGWARFVIPAGSRYRSPGEIHVEADASSASYFIAAGALSASASGPNGLKIEGVGE
                     ASIQGDIRFVEAARLMGAQIDSGPNWLRAERGAWPLKAIDLDCNHIPDAAMTLGVMAL
                     YADGPSTLRNIASWRVKETDRIAAMATELRKLGAEVEEGADFIRITPPSVWQAASLHT
                     YDDHRMAMCGSLAAFNAAGLPVRIEDPKCVAKTFPDYFETLFELAETAAPVPVICIDG
                     PTASGKGTLASEVARRLGYHYLDSGSLYRLTGLAARRAGLSLENPDEARLTTLAQQLP
                     VRFVADRILLAGEDVSDAIRTEAAGMDASRVSALPGVRQALLDLQRGVARLPGLVADG
                     RDMGTVIFPHAPLKIYLTAGAPERAERRHKQLISKGIPAKLDSLRADLEARDARDQTR
                     AAAPLKPAQDALLLDNSHLTIEQSVDQVLNWWQERQPFRPS"
     gene            complement(7610..8482)
                     /locus_tag="PKV30_02985"
     CDS             complement(7610..8482)
                     /locus_tag="PKV30_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRL29218.1"
                     /translation="MFEQLGLIGCGLMGGSFALALKRAGLVRRVVGYSKSPSTTERAR
                     QLGVIDIEAPSALLAVSGADLVLLAVPVAATEATLKAIRHLVNPDMLIMDVGSSKRDV
                     VDAARRVLKHDVGVFVPCHPITGREVSGVENADAGLYQGRQVILTPIERTLTTKLDKA
                     RALWQALGCEVLEMSPESHDAAFAAVSHLPHLVAFALMNSIADQPEGQDYLSMAGPGF
                     RDFSRIAASDPQMWRDVLIANREELGRQTALFQQALADLQKLIASGQADDLERLINRA
                     SHARANWRVSPGAR"
     gene            complement(8475..9584)
                     /gene="pheA"
                     /locus_tag="PKV30_02990"
     CDS             complement(8475..9584)
                     /gene="pheA"
                     /locus_tag="PKV30_02990"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRL29219.1"
                     /translation="MSLPPPIQSEALAKLRTQIDRLDAELLSLLNQRARVAEEVGELK
                     RHEGTPFFRPDRVAQVIAKVQASNPGPLQNQHVASIWREIMSACLALEVPQRVAVLGP
                     QGTFCEQAAIEFFGSAANLIYCNSFDEVFHATAAGTSQFGVVGMENSTEGVVARSFDL
                     FLHSPVHVVGEVSLLVRHNLLRQVNSLEGIEVVLAHPQALMQCQGWLSQHLPKAERRA
                     VSSNAEGARLAATNPAWAGLASERAAAQFGLHIVSHAIQDEAYNRTRFSVICLPQTLA
                     MPAATARDCTSIVVSVPNKPGSVHDMLTPLKKHGVSMTRFESRPAKSGKWEYYFYIDL
                     QGHPSQANVAAALSELQAYCAFYKMLGAYPLGQDV"
     gene            complement(9654..10127)
                     /locus_tag="PKV30_02995"
     CDS             complement(9654..10127)
                     /locus_tag="PKV30_02995"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HRL29220.1"
                     /translation="MATKLAQIQLKADGAAMAEQLTASAVQPVIAGWSQRLDETVPPA
                     KQKEVRDKLDVELKKFAETTHKTVEGQVGKTAEAAMVPIFMEKLSEDELKTIIAYLES
                     PVSAKFQSLGPDATNAWAKRIIDATKPAVESGVKTFDASANRIVGAAAGAAPAKK"
     gene            complement(10230..12887)
                     /gene="gyrA"
                     /locus_tag="PKV30_03000"
     CDS             complement(10230..12887)
                     /gene="gyrA"
                     /locus_tag="PKV30_03000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRL29221.1"
                     /translation="MTSFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFSMHEANNVWNRPYVKCARVVGDVLGRFHPHGDLATYEALVRMAQDFSMRYPL
                     IDGQGNFGSVDGDSAAAYRYTECRLEKISSEILADIDKQTVDMVPNYDGKELEPTVLP
                     TRLPNLLINGSGGIAVGMATNIPPHNLNEVVDACLHLLRQPDATIDELMDIVPAPDFP
                     TGGIIYGVQGVKDGYRTGRGRVVMRAKVHFEDIDKGQRQAIIVDEIPYQVNKKTLQER
                     MAELVHEKKLEGISHIQDESDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGVNM
                     VALIDGQPKLCNLRELIQVFLQHRREVVTRRTVYDLRKARERGHVLEGLAVALANIDE
                     FIRIIRESPTPPVAKTELMARSWDSALVREMLTRTREDGVTISADDYRPDDLDRIYGL
                     QPDGLYRLSDTQAQEILQMRLQRLTGLEQDKIVAEYKEVMAEIEDLLDILAKPSRVST
                     IISDELTALKAEFGQTKQGARRSIIEHNAQDLATEDLITPTDMVVTLSHTGYIKAQPL
                     SEYRSQKRGGRGKQATATKEDDWIDQLFIANTHDYILCFSNRGRLYWLKVWEVPSGSR
                     GSRGRPIVNMFPLQEGEKINVILPLTGDNRSFPADHYIFMATAGGTVKKTALDEFSNP
                     RKIGIIAIKLDEGDQLIGAAVTDGQHDVMLFSDGGKAVRFDENDVRALGRDTRGVRGM
                     QLEEGQSVIAMLVADAEAGDGSAEPNARNSVLTATENGYGKRTNITEYTRHGRGTKGM
                     IAIQQSERNGKVVAATLVSVEDEIMLITDKGVLVRTRVAEIREMGRATQGVTLIALDD
                     GAQLSGLQRIVENDAQEAAPDEPSESPTE"
     gene            13122..>13629
                     /locus_tag="PKV30_03005"
     CDS             13122..>13629
                     /locus_tag="PKV30_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL29222.1"
                     /translation="MKKLNKVAIMFAVATVAGSAFAAGSTINSDNWRSGHGNLIWKNG
                     TNELCWRDAFWTPATAAPGCDGALEAPKAAAPAPAPAAAPVAPAPAPAPAPVAAQKVT
                     YAADAFFDFDKSVLKPEGRAKLDDLVSKIQGINLEVIIAVGHTDSVGSDAYNQRLSVR
                     RAEAVKAYL"
BASE COUNT         2212 a         4338 c         4567 g         2512 t
ORIGIN      
        1 gcgaataccc cagccgcaac ccgcagcaga acatggccga cctgaaggcg cagattgccg
       61 ccaacgaaaa aggcgtgcag gagctgcgca agatggtcga tcagttcggc ctcgatgtgg
      121 tgcaggccta catgaagcac gtgcaggaca acgccgaaga aagcgtgcgc cgcgccatca
      181 ccaagctggc ggcgcgcatg cagggcggcc agttcacgct gccgctggac aacggggccc
      241 agattcaggt cgccgtgacc atcgacggcg ccgcgcgcag tgccaaggtc gatttcaccg
      301 gcacttcgcc gcagcagatc aacaacttca acgcgcccac tgcggtgtgc atggcggcgg
      361 tgctgtacgt gttccgcatg ctggtggatg acgacattcc gcttaacgcc gggtgcctga
      421 agcccatcga ggtcgtcatc cccgaaggca gcatgctgcg gcccaacccg ccggccagcg
      481 tggtggcggg caatgtggaa acgtccacct gcatcaccaa cgccatcatc ggcgcgctcg
      541 ggctgatggg cgccagccag tgcacggtga acaacttcac cttcggcaac gacaagtacc
      601 agtattacga aaccatctcg ggtggctcgg gcgcgggcgg cgtgtttgac gacgccgggc
      661 agctggtcgg cggcttcccc ggcaccagcg tggtgcagac gcacatgacc aattcgcgca
      721 tgaccgatcc ggaggtgctg gaatggcgct tcccggtgcg gctggagagc tatgaaatcc
      781 gcagcgactc aggcggcaag ggccaatgga acggtggcca cggcggcgtg cggcgcctgc
      841 gctttctgga gcccatgacc gccagcatcc tcagcaacgg ccgcctgcgt ggcgctttcg
      901 gcatggccgg cggcggcgcg ggcgcggtcg gcatcaaccg ggtggtgcgt gccagcggca
      961 cggtgcagga cttgcagcac atcggcagcg ccgagatgca gcccggtgac atcttcgaga
     1021 ttcacacgcc gggcggcggc gggtttggcg ccgtctgaac gtgtgttgat ggccaaaaag
     1081 gccgccagcg caaaccagac aagcgcaagc agctattaaa aataaagcaa gaaggggcgc
     1141 gtgtgaacgt gccccttctt gctgccgctt gcgctggccg gctggccaga ccaagcaggg
     1201 ctagaactta ctgcttgacg gcaggcgccg caggtttggc cggtgcggcg ggcgctgcgg
     1261 gcatggccgg cgcggcgggt ttggtggcga tggcagggat cgcggccgtg gtgcctgcag
     1321 cggcgggcgt ggcgccagcg gccggcgctg cggccacggt tttctccttc ttggccttcg
     1381 cgtccttggc cggcttggcg ggcgcacccg cgtagcccgc gccgttgacc gtcatgccgc
     1441 cggcggaaaa gttggcggca ttcttctcgc cgacgccttt cacgcgggcc atgaagtcgc
     1501 cccagtcctt gaactggccc ttggcccgtt cgttgatgat gcgcttggac atgcccgggc
     1561 cgatgccctt gacgctgtcc aggtcggcgg cactgccggt gttgacgtcg acggccgcga
     1621 acgagagcgc ggcgtagaac agacttaaaa ggacgagaag tttcttgaac atgggaacgc
     1681 tcctggtggc gtgcgaacaa tgccggctga agccgccggc gggcctttgc gctcaacggc
     1741 cgagcgcacc gggccgttga cggcaacggg gactacaact cttcgatgcg ccgcggcgga
     1801 aagctttccc aagcctggca gccggggcac tgccagaaat agccctttgc ctcgaacccg
     1861 caggcggcgc agcggtagcg cgccagcggc cgcgcggcgt gctcgaccga gcgttgcacc
     1921 acggggtgcg cgccttcctg cgcaaacgcc tcctggctca accagcgcgc cgccgcgatc
     1981 agcgagggct cctgcgccat gtggcgcacg tagccggcgc gcgcatcgtg caacggtgtc
     2041 cccgccgtga cctcggcctg caccagcgcg tccgcgacgt ccaccgaagg cgtggcggcg
     2101 tagctgcgct ccagcagttg ccgcgcctgc gccagttggc cgctggcgtg gccaatgcgc
     2161 accaactcgc tggcgaccag cggcacggcc tgcggcgcgt ggtgctccag gctgatcagt
     2221 tgttcgaagg cgccgctcca gtcgtcgcgg ccggccagca aaccggccag ctggatgcgc
     2281 gggcgcgccg cgcttggcgt ttgttcgatg gcttgcagca gcagttgctg ggcggcctgg
     2341 gcatcgtgct tcttgttcag cgcctctgcc gcctgctcgc acaggtagtg cgcctgccgc
     2401 gcgctgaaat cggcctggcc agaggcctgc aggcgaccag cgacggttgc agcctgcggc
     2461 cagtcgcgcg cgcgctcgtg tagcgacaac aatgtgagcc aggcctgctc ctgatagcgt
     2521 gtgccctcga gcttgcgcaa ggcgtcttcg gcgcggtcga gcaagccggc tttcagaaaa
     2581 tcctgcgcca gtgcgtgctg cgcgcggtcg cggtcgctct ggctcaggtc ggcgcgtgag
     2641 agcaggtgtt cgtgcacgcg cacggcgcgc tcgtattcgc ctcggcgacg aaacaggttg
     2701 cccagggcga aatgcagctc tgacgtgtca gggtcttttt gcaccgcctc gatgaaggcg
     2761 tcgatggcct ggtcttgctg ctcgttcagc aggtagttga ggcccttgaa atacgctttg
     2821 ggcgcacgct ggttttcgat gcgcaactgg cgcagatcga gccttgacgc cagccagccg
     2881 agcgcgaaca cgagcggcac accgagcagg atccagctga ggtcaaactc catggggcgg
     2941 gctggtcaac tggtcgtcca gcggcgccag atcagagcgc gctggctctg catgtccccg
     3001 cacgttgcgg cgcgaacgcc agccgggcag catgcccagc acacccacca ccagacccag
     3061 cgcgaaagcg gccagcacga tcagcatcat cggcgcgcgc cactggtggc cgaacagcaa
     3121 gtgcacggtg gcgtcctgct ggttgttcag cgcaaaagcg aaaagggcaa aaaaaacggc
     3181 tgccttcagt atccacgtca gcagccgggt gagctttgtc atgcgtcttc tgggagaggt
     3241 cgaagacgat tctagcggtc gatccctcag ggcccggcat cccccgcatc gacgggttgc
     3301 gcgccgacgg cttcacgcaa ggccttgccc ggcttgaagt gcggcacgcg ccgctcgggg
     3361 atgtgcacac tctcgccggt gcgcgggttg cggcccaggc gcggcggccg gcgattgatc
     3421 gagaagctgc cgaagccgcg gatctcgatg cggtgtccac ggaccagtgc gtcgctcatc
     3481 gcgtcaagaa tggtcttgac tgctgcatcg gcatcgcgct gcgtcaactg gccgaagcgc
     3541 tcggccagca gttcgacgag atcggaacgg gtcatgtaac ggatatcacc tcattcggaa
     3601 gcgatgctgc gtcgcgctcc ttctcagggc acccgtggag cgggcttggc ccggccactg
     3661 ggtgcgcccc ttgaggggag gcgccgcagg cgcttcgggg tgggctttac ttgtcgttat
     3721 tgtccagctt ggcacgcagc agcgcgccca ggctggtggt gcctgccgtg ccggtgttct
     3781 ggttcaggct ggccatggct tcctgctggt cggcggcgtc cttggccttg acggacaact
     3841 ggatgttgcg cgtcttgcgg tcgacgttga ccaccacggc ggtcacttcg tcgcccactt
     3901 tcaggacctg gctcgcgtct tcgacacggt cgcggctgat ttcggaagcg cgcaggtaac
     3961 cgatgacgtc ctggcccagg tcgatttcgg cgccacgggc gtccacggtc ttgacggtgc
     4021 cgctgacgat ctggcccttg tcgttcaccg tcacgaaggt ggtgaacggg tcctggtcga
     4081 gctgcttgat gccgaggctg atgcgctcgc ggtcgacgtc aacggccagc acgatggctt
     4141 cgacttcctg gcccttcttg tagttgcgca cggcggcttc gccggtttcg ttccacgaca
     4201 ggtcggacag gtgcaccagg ccgtcgatgc cggcagccag acccacgaac acgccgaagt
     4261 cggtgatcga cttgatgggg cccttgacgc ggtcaccgcg cttggtgttc tgcgcgaact
     4321 cttgccacgg gttggcgcgg cactgcttca tgcccagcga gatgcggcgc ttgtcttcgt
     4381 cgatctcgag caccatgacc tcgacctcgt cgcccagcga cacgatcttc gacggcgcga
     4441 cgttcttgtt ggtccagtcc atctcggaca cgtgcaccag gccctcgatg cccggctcca
     4501 gctcgacaaa cgcgccgtag tcggcgatgt tggtgacctt gccgaacatg cgcgtgcctt
     4561 gcgggtagcg gcggctcacg cccagccatg ggtcgtcgcc catctgcttg aggcccagcg
     4621 acacgcggtt cttctcggtg tcgaacttga ggatcttggc tgtgatctcc tggcccgcct
     4681 gcaccacctc ggacgggtgg cgcacgcggc gccaggccat gtcggtgatg tgcagcaggc
     4741 cgtcgatgcc gccgaggtcc acgaacgcgc cgtattcggt gatgttcttg accacgccgt
     4801 tgaccacggc gccttctttc agcgtctcca tcagcttggc gcgctcttcg cccatgctgg
     4861 cttcgaccac cgcacggcgc gacagcacca cgttgttgcg cttgcggtcg agcttgatga
     4921 ccttgaactc aagcgtcttg ttctcgtaag gcgttaggtc cttggtggga cgggtgtcga
     4981 ccagcgagcc gggcaggaag gcgcgaatgc cgttgaccag caccgtcagg ccgcccttga
     5041 ccttgccgct ggtggtgccg gtgacgaagt cgccagattc gagcgctttc tccagcgcca
     5101 gccacgaggc caggcgcttg gcggtgtcgc ggctcaggat ggtatcgccg tagccgtttt
     5161 cgatcgagcc gatggccacc ggcacgaagg cgccgggctc gacctcgacc tcgccttggt
     5221 cgttcttgaa ttcctcgatc gggatgtagg cctcggactt gaggccggca ttcacgacga
     5281 cgaagctgtg ctcgacgcgc acgacttcgg cggtaatgac ttcacccggg cgcatttcgg
     5341 tgcgctgcag ggattcttcg aacaactggg caaaagattc agacatgaaa ggtttccttg
     5401 tccgcgcgca ggttgacgaa cacacgattg cattcgtttg aaagactggc gacggaagct
     5461 tgggtttgcg gacgatccgc ggttagggtg aaaaaccagt cggctcgatg cgcttgaacg
     5521 cgcggcggga agccaactgg gcaaaaaacc cgtccggacg tggtcttgac gggtgtgcgg
     5581 gcaaccaagg ccgtcaggat ggacggaagg gctgcctctc ttgccaccag ttcagcactt
     5641 gatcgaccga ttgctcaatc gtgaggtgcg agttgtccag cagcagggcg tcttgcgcgg
     5701 gtttgagtgg cgcggcggcg cgggtctgat cccgcgcatc gcgcgcttcc aagtcagcgc
     5761 gaagactgtc aagtttagca ggaataccct tggaaatcaa ttgcttatgg cgcctctccg
     5821 cacgctcggg cgcgcccgct gtgaggtaga tcttgagcgg cgcgtgcgga aagatcaccg
     5881 tgcccatgtc gcggccatcg gccaccaggc cgggcaggcg agcaacgccg cgctgcaaat
     5941 ccaggagcgc ctggcgcacg cctggcaagg cggagacgcg ggaagcgtcc atgcccgcgg
     6001 cttcggtgcg gatggcgtcg ctgacgtcct cacccgccag cagaatgcgg tccgccacaa
     6061 aacgcaccgg caattgctgc gccagcgtgg tcaggcgcgc ctcgtccggg ttttcaagcg
     6121 acagccccgc gcgccgggcc gccaagccgg tgaggcggta cagcgagccg gaatcgaggt
     6181 agtgatagcc cagccggcgc gccacctcac tggccagcgt gcccttgccg gacgccgtgg
     6241 ggccgtcgat gcagatcacg ggcaccgggg cggcggtttc ggccagctcg aacagcgttt
     6301 cgaagtaatc ggggaaggtc ttggccacgc acttcgggtc ttcgatgcgc accggcaggc
     6361 cggccgcgtt gaaggcggcg agcgagccgc acatggccat gcggtggtcg tcgtaggtgt
     6421 gcagcgacgc ggcttgccag accgaaggcg gcgtgatgcg gatgaagtcg gcgccttctt
     6481 cgacctcggc gcccagcttg cgcagctcgg tcgccatggc ggcgatgcgg tcggtttcct
     6541 tgacgcgcca cgacgcgatg ttgcgcagcg tgctggggcc gtcggcgtac aaggccatca
     6601 cgcccaaggt catggcggcg tcggggatgt ggttgcaatc caggtcgatg gccttgagtg
     6661 gccaggcgcc gcgctcggcc cgcaaccaat tggggccgct gtcgatctgt gcgcccatga
     6721 ggcgggccgc ttcgacgaag cggatgtcgc cctggatcga ggcttcgcca acgccctcga
     6781 ttttcaagcc atttgggccg ctggcgcttg cggatagagc gccagcagct atgaaataac
     6841 tagcggacga ggcatcggcc tcgacgtgaa tttcgcccgg cgagcggtag cggctgccgg
     6901 ccgggatgac gaagcgcgcc cagccgttgc gctgcacctt gacgccaaag cgcgccagca
     6961 actgcagcgt gatctcgatg tagggctttg agatcagctc gcccaccacg tcgatgacga
     7021 tgtcctgcgc ctgggccgcc agcggcagcg ccatcagcag cgcggtgagg aactggctgg
     7081 agacgtcgcc gcgcacaggg atcggctgat caagtcggag attcggctgg ccgatgtgca
     7141 agggcggaaa gccgtcctga cccagatact gcacgttgca gcccagcgcc cgcagcgcat
     7201 cgaccagatc gccaatcggc cgttcgtgca tgcgcggaat gccgcttaaa gcgtagtcgc
     7261 cgcccagtac cgccagcgct gccgtgagcg gccgcatcgc ggtgccagcg ttgccaagga
     7321 acagctcggc ctgtggctgg cgcgcgcggc cacccagtcc gtcgatgacc gccgtcgtgc
     7381 cgtcgacggc cacgccgcag cccaactggc gcagcgcctg cagcatgacg cgcgtgtcgt
     7441 ccgaatcgag caggtcgcgc acggtggttt gcccctcgca cagcgccgcc aacagcagca
     7501 cgcggttcga gatgctcttg gagccgggca ggcgcaccgt gcccgacacg acgcgcaacg
     7561 ccggaacgtc gaggaactca gtctcgaaca cggacgagca ccacgcgcct cagcgcgcgc
     7621 cgggcgacac gcgccagttg gcgcgcgcgt ggctggcgcg gttgatcagg cgctccaggt
     7681 cgtcggcctg gccgctggcg atgagctttt gcagatcggc cagcgcttgc tggaacagcg
     7741 cggtctggcg cccgagttct tcgcggttgg cgatcaacac gtcgcgccac atctgcgggt
     7801 cgctggcggc gatgcggctg aaatcgcgaa agcccgggcc agccatggac aggtagtcct
     7861 ggccctcagg ctgatcggca atgctgttca tgagcgcgaa cgccaccagg tgcggcagat
     7921 ggctgacggc ggcaaaggcg gcgtcgtgcg attcgggcga catctccagc acctcgcacc
     7981 ccagcgcctg ccagagcgcg cgcgccttgt cgagcttggt ggtcagcgtg cgctcgatgg
     8041 gcgtcaggat gacttgccgg ccctggtaca ggccggcgtc ggcgttttca acgcccgaga
     8101 cctcgcgccc ggtgatcggg tgacagggca cgaagacgcc gacatcgtgc ttcagcacgc
     8161 gccgcgcggc atccaccacg tcgcgcttgc tggagcctac gtccatgatc agcatgtccg
     8221 ggttgaccag gtggcggatg gccttgagcg tggcctcggt cgccgccacc ggcacggcca
     8281 gcagcaccag atcggcgccc gacaccgcca gcagcgcgga gggcgcctca atgtcgatca
     8341 cgcccagctg gcgcgcgcgc tcggtggtgg agggcgattt gctgtagccg accacgcggc
     8401 gcaccagccc ggcgcgcttg agcgccagcg cgaaagaccc gcccatcagg ccgcagccga
     8461 tcagtccgag ttgttcaaac atcttgtccg agggggtagg cgccgagcat cttgtagaac
     8521 gcgcagtagg cttggagttc agacagcgcc gcggctacgt tggcttggct cgggtggcct
     8581 tgcaggtcga tgtagaaata gtattcccat ttacccgact tggcggggcg cgactcgaag
     8641 cgcgtcatcg atacgccgtg ctttttcaac ggagtcagca tgtcgtgcac cgaaccgggc
     8701 ttgttgggca ccgacaccac gatgctggtg cagtcgcgcg ccgtggccgc cggcatggcc
     8761 agcgtctggg gcaggcagat gaccgaaaag cgcgtgcggt tgtacgcctc gtcctggatc
     8821 gcgtgcgaca cgatgtgcag gccgaactgg gccgctgccc gctcgctggc caggcccgcc
     8881 caagcggggt tggtggccgc cagccgcgcg ccttcggcat tgctggagac ggcgcggcgc
     8941 tcggccttgg gcagatgctg cgacagccag ccctggcatt gcatcagcgc ctgcgggtgg
     9001 gccagcacga cttcgatgcc ttcgagcgaa ttgacctggc gcagcaggtt gtgccgcacc
     9061 agcaggctga cttcgcccac cacgtgcacc ggcgaatgca gaaacaggtc gaacgagcgc
     9121 gccaccacgc cttcggtgga gttctccatg ccgaccacgc cgaactgtga cgtgccggcc
     9181 gccgtggcgt ggaacacctc gtcgaagctg ttgcagtaaa tcaggttggc ggcgctgccg
     9241 aagaactcga tcgccgcctg ctcgcagaac gtgccttgcg gccccagcac ggcgacgcgc
     9301 tgcggcactt cgagcgccag gcaggccgac atgatctcgc gccagatcga ggccacgtgc
     9361 tggttctgca gcgggccggg gttgctcgcc tgcaccttgg cgatgacctg cgccacgcga
     9421 tcaggccgaa agaaaggcgt gccttcgtgt cgcttgagtt cgcccacttc ctcggccacc
     9481 ctggcgcgct ggttgagcag cgacaacagt tccgcatcca gccggtcgat ctgcgtgcgc
     9541 agcttggcaa gggcttcgga ctggatgggc ggcggcaaac tcatgcggca gagcggctca
     9601 tgaaaatgta gcgcgccggc aaggacgccg gcgcggtgag gacatgggtg actttacttc
     9661 ttcgccgggg ccgcaccagc cgcggcgccg acgatgcggt tggctgacgc gtcgaaagtc
     9721 ttcacgccgc tttcaaccgc cggcttggtg gcatcgatga tgcgcttggc ccaggcgttg
     9781 gtggcgtcgg gcccgagcga ctgaaacttg gcgctgaccg gcgattcgag gtaggcgatg
     9841 atggtcttca gctcgtcttc gctcagcttt tccatgaaga tcggcaccat ggccgcttca
     9901 gcggtcttgc cgacctgacc ctcgaccgtc ttatgcgtgg tctcggcgaa cttcttcagc
     9961 tcgacgtcca gcttgtcgcg cacttctttc tgcttggccg gcggcacggt ttcgtccagg
    10021 cgctgcgacc agccggcaat gaccggctgc acggcgctgg ccgtcaactg ctcggccatg
    10081 gccgcgccgt cggccttcag ctgaatctgc gccaacttgg tggccaatgc acgttttgca
    10141 tcattttggg ccagagcgct cgtagatact gcgcaagcag ctaccaaaag cagagcggcg
    10201 ggaacacggg tgaaagtgat tttcaaggct tactccgttg gggattctga gggttcatcg
    10261 ggcgcggctt cctgcgcgtc gttttcgaca atgcgctgaa ggccgctgag ctgggcgccg
    10321 tcgtccaggg cgatcagggt gacgccttga gtggcgcgcc ccatctcgcg gatctcggcc
    10381 acacgggtgc gcaccagcac gcccttgtcg gtgatcagca tgatctcgtc ttcaacgctt
    10441 accagggtcg cggcgacgac tttgccgttg cgttcgcttt gctggatggc gatcatgcct
    10501 ttggtgccgc ggccatgccg tgtgtattcg gtaatgttgg tgcgctttcc gtaaccgttt
    10561 tcggtggcgg ttaggacgct gttgcgcgca ttaggttccg cactgccgtc gccggcctcg
    10621 gcgtcggcca ccagcatggc gatgaccgac tggccttctt ccagctgcat gccgcgcacg
    10681 ccacgcgtgt cacggccgag cgcgcgcacg tcgttctcat cgaagcgcac tgccttgcca
    10741 ccgtcggaga acagcatcac gtcgtgctgg ccatcggtga cggcggcgcc aatcagctga
    10801 tcgccttcgt cgagtttgat ggcgatgatg ccgatcttgc gcgggttgct gaactcgtcc
    10861 agcgccgttt tcttgaccgt gccgccggcg gtggccatga agatgtagtg atcggccggg
    10921 aagctgcggt tgtcgcccgt caacggcagg atcacgttga tcttctcgcc ttcttgcagc
    10981 gggaacatgt tgacgatcgg gcgcccgcgc gagccgcgcg aacccgaggg cacttcccac
    11041 accttgagcc agtacagccg cccgcggttg gaaaagcaca ggatgtagtc gtgcgtgttg
    11101 gcgatgaaga gctggtcgat ccagtcgtct tctttcgtgg ccgtggcctg cttgccgcga
    11161 ccgccgcgct tttgcgaacg gtattcgctc aaaggctgcg ccttgatgta gccggtgtga
    11221 ctgagcgtga ccaccatgtc ggtgggtgtg atgaggtctt cggtggctag atcttgcgcg
    11281 ttgtgctcga tgatggagcg gcgcgcgcct tgcttggtct ggccgaactc ggccttgagt
    11341 gcggtcagct cgtcgctgat gatggtcgac acgcgcgacg gcttggctag gatgtcgagc
    11401 agatcctcga tctcggccat cacttccttg tattcggcga cgatcttgtc ctgctccagg
    11461 ccggtcagac gctgcaggcg catttgcagg atctcttgcg cctgcgtgtc ggagaggcgg
    11521 tacaggccat ctggctgcag gccgtagatg cggtccaggt cgtcgggccg gtagtcgtcg
    11581 gcgctgatgg tgacgccgtc ctcgcgcgtg cgggtgagca tctcgcgcac cagcgcgctg
    11641 tcccaactgc gcgccatcag ctcggtcttg gcgaccgggg gcgtgggcga ctcgcggatg
    11701 atgcggatga actcgtcgat gttggccagc gccaccgcca ggccttccag cacgtggccg
    11761 cgctcgcgtg ccttgcgcag gtcgtacacg gtgcggcgcg tgaccacttc gcggcggtgc
    11821 tgcagaaaaa cctgaatcag ctcgcgcaga ttgcacagct tgggctgacc gtcgatcagc
    11881 gccaccatgt tgacgccgaa ggtgtcctgc agctgcgtct gcttgtacag gttgttcagc
    11941 accacctcag gcacttcgcc gcgcttgagt tcgatgacca ggcgcatgcc ggacttgtcg
    12001 gactcgtcct ggatgtggct gatgccttcg agctttttct cgtgcaccag ctcggccatg
    12061 cgctcttgca gcgtcttctt gttgacctgg taaggaatct cgtcaacgat gatcgcttgg
    12121 cgctggccct tgtcgatgtc ttcgaagtgc accttggcac gcatcaccac gcgtccgcgc
    12181 ccggtgcggt agccgtcttt gaccccctgg acgccgtaga tgatgccgcc ggtgggaaag
    12241 tcgggcgcag gcacgatgtc catcagttcg tcgatagtcg cgtcgggctg ccgcagcagg
    12301 tgcaggcagg cgtccaccac ctcgttcagg ttgtgcggcg gaatgttggt cgccatgccg
    12361 acggcaatgc cgcccgaacc gttgatcagc agattgggca ggcgcgtcgg cagaacggtc
    12421 ggttccagtt ccttgccgtc gtagttgggc accatatcga cggtctgctt gtcgatatcg
    12481 gccaggatct cgctggatat cttctccagt cggcactctg tgtagcggta ggcggcggcg
    12541 ctgtcgccat cgaccgagcc gaagttgccc tggccgtcga tgagtgggta gcgcatcgag
    12601 aagtcctgtg ccatgcgtac cagggcttcg taggtggcca agtcgccgtg cgggtgaaaa
    12661 cggcccagca cgtcgcccac cacgcgcgcg cacttgacat acggccggtt ccagacgttg
    12721 ttggcctcat gcatcgagaa caacacgcgg cggtgcaccg gcttgaggcc atcgcgcgca
    12781 tcaggaagcg cacgacccac gatcacgctc atggcgtaat ccaggtagct tcgccgcatc
    12841 tcctcttcta gactgatggg gagcgtctct ttggcgaatg aagtcatata tataggcggt
    12901 cggctcgggc agacgaaagc ggctgatttt aggcggttta tggtgtcgct tcgtcgcaac
    12961 attcggccgg aattgaggcc ctgtctgacg cgcgcggctg tcgttctgtt aagaatgcac
    13021 aaagacgtgt ggcacaatca atccaacgtt tttggtggtg gtcacttgaa gcgtccgatt
    13081 tatcgcagcc cggctgcggc ttttttccca tgaggagaac catgaagaaa ctgaacaaag
    13141 tagcgatcat gttcgctgtt gcaaccgtgg ctggttccgc cttcgctgct ggctcgacga
    13201 tcaattccga caactggcgc agtggccacg gcaacctgat ctggaagaac ggcaccaacg
    13261 aactgtgctg gcgtgatgcc ttctggacgc ctgccaccgc cgcccctggc tgcgatggcg
    13321 ctctggaagc gcccaaggcc gccgcaccgg cgccggctcc cgctgctgcg cctgtcgcac
    13381 ctgctccggc ccctgctccg gcacctgtgg cagcacagaa agtgacctac gctgccgacg
    13441 cattcttcga ctttgacaag tcggtgctca agcccgaagg ccgcgccaag ctggacgatc
    13501 tggtcagcaa gatccagggc atcaacctgg aagtgatcat tgccgtcggc cacaccgact
    13561 cggtgggcag cgacgcttac aaccagcgtc tgtcggtgcg tcgtgccgaa gccgtgaagg
    13621 cctacctgg
//
LOCUS       DAOMVO010000031        13151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1071_length_13151_cov_16.885232, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000031 DAOMVO010000000
VERSION     DAOMVO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_13151_cov_16.885232"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..517
                     /locus_tag="PKV30_03010"
                     /pseudo
     CDS             1..517
                     /locus_tag="PKV30_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830938.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
     gene            514..2766
                     /locus_tag="PKV30_03015"
     CDS             514..2766
                     /locus_tag="PKV30_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRL29223.1"
                     /translation="MKLRKELWFGFTRMALIIAAALGMLVSVEKITNGHVGLLMLSLV
                     VVAIMLGFPTAFTLMGMGMLFTWLAYDRDWHRTLDLMVQSAFKVMGNDVLIAVPLFVF
                     MGYLVERANLIQKLFHSLHLSMARVPGSLAVATLVTCAVFATATGIVGAVVTLMGLLA
                     LPAMLRAGYSVPLSAGAITAGGCLGILIPPSVLLIVYGATAGVSVVQLYAGAFFPGLM
                     LVGLYVLYVIIVAKLKPAWAPPLSASDRLVALAAPLVSLGARNEQGAMGFMLRTLRRG
                     DSAAEKNYLRKQLFVALVPALLFAAMAFWSYQLFTAEERVDEVPAELERMGAGGEATS
                     SQGGLAEPPGSGESAAPSGSGLAEPPTEPSPPAEGGLAEPPGSSDTPAAASDASGLAE
                     TPGAAPAADAPTTAGSLAEPPAVGAQPGGASATPTPQTSAAEAPSAPRGYWITVGLGA
                     LALLAYYALLNFNRLEIFKMLLSSFFPLVILILSVLGSIVFGLATPTEAAAVGAFGGY
                     VLACIYALVGKRKGRVGATLALWLLLWLPALVAIGWFLLGRGESNASPVPPWLGWLAV
                     AGIGVWCVVGYRQAGLQGEVKESVYLTAKTSAMVCWLFVGSAIFSAAFALLGGQELME
                     QWVLGMNLSKTQFLILTQVIIFILGWPLEWTEIIVIFMPIFVPLLDNFGVDPLFFGLL
                     VALNLQTAFLSPPVAMAAFYLKGVSPPHVTLNQIFLGMLPFMGIQILAIVLLYVFPQI
                     GLWLPQILYK"
     gene            2883..3725
                     /locus_tag="PKV30_03020"
     CDS             2883..3725
                     /locus_tag="PKV30_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--nucleotide phosphotransferase"
                     /protein_id="HRL29224.1"
                     /translation="MAKKTDAQASHDAARWQVPIGGKGFDLKDCDPAAKPCSSGDKTA
                     DKTRVTDLAREIDALQDVFWADKRFKLLVLLQGTDTAGKDGTIRAVFKETSPLGVRVV
                     GWKAPSELERSHDYLWRIHHEMPASGEFMIHNRSHYEDVLVPVVERWIDKKTTEQRYR
                     QINDFERMLSENGTVIMKFMLHIGFDEQRQRLQERIDDPAKHWKFSLGDLDARKQWPL
                     YQQAYDHLLRATSTEWAPWTVVPADSKTHRNLMIATLVRDKLKSLKLRYPPDNPELKG
                     LRVQ"
     gene            3766..7137
                     /locus_tag="PKV30_03025"
     CDS             3766..7137
                     /locus_tag="PKV30_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRL29225.1"
                     /translation="MTDLSAEFKALAEYRPTNKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVIHLGHNRSVDEVVTAALQEDAQGIAISSYQGGHVEYFKYMVDLLNSRGGAHV
                     QVFGGGGGVIVPAEIRELHDYGVARIYSPEDGQRMGLQGMIGEMVMRCDQDLSGYAPT
                     AIEAIQGHGEAAWRALAQLITALENEKADEALVAAVRAQAATKKIPVVGITGTGGAGK
                     SSLTDELIRRLRLDQDDALRVAVISIDPSRRKSGGALLGDRIRMNAIGPWTQPTVRPE
                     PVEVQAEPSTSSARTDLGQRVYMRSLATRDTGSEISPALPDVIAACKAAGFDLIIVET
                     SGIGQGDAAIVPHVDVPMYVMTPEYGAASQLEKIDMLDFAEFIAINKFDRKGAQDALR
                     DVAKQVQRNKSAFNVRPEEMPVFGTQAARFNDDGVTALYQALKVRLGELGLPLKEGRL
                     PPVNVRHSTNQTPIVPPARTRYLAEIADTVRGYKQRVRAQARLVREVQQLRETARMLQ
                     ADNPDKTGEHGARATVLKLADERATRVDRDAAKLIEQWPAMQQAYAGDEYVVKIRDKE
                     IRTALTTKSLSGTTIRKVVLPQFEDHGEIVKWLMLDNVPGSYPYTAGTFAFKREGEDP
                     TRMFAGEGDAFRTNRRFKLVSEGMPAKRLSTAFDSVTLYGNDPDPRPDIYGKVGNSGV
                     SIATLDDLKVLYSGFDLCNPTTSVSMTINGPAPSILAMFMNAAMDQQLEKFQADNGRQ
                     PTDTEAEKIKEWTLANVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIAEYFVHHNV
                     RNFYSVSISGYHIAEAGANPISQLAFTLSNGFTLVEAYLARGMHIDDFAPNLSYFFSN
                     GMDPEYTVMGRVARRIWAVAMKEKYGANERSQKLKYHIQTSGRSLHAQEIQFNDIRTT
                     LQALIAIYDNCNSLHTNAFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPNQGAF
                     VIEELTELVEEAVLSEFEKIADRGGVLGAMETGYQRSKIQEESMHYEMLKHTGEYPII
                     GVNTFRNPKGDEVLESLELARSTEDEKRSQLARLADFHQRHAGEAPAMLARLQQAVID
                     NGNVFAVLMDAVRVCSLGQITNALFEVGGQYRRSM"
     gene            7261..9045
                     /gene="ggt"
                     /locus_tag="PKV30_03030"
     CDS             7261..9045
                     /gene="ggt"
                     /locus_tag="PKV30_03030"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRL29226.1"
                     /translation="MTLQPRLLLGSIALAVLTACSTATAPPAQAPAATAPTTTSATQA
                     AAAAYDWDMDVFHPVVARNGMVASEQELASQIGLNVLKSGGNAVDAAVAMGFALAVAL
                     PNAGNLGGGGFMMVHDAKTGKDVAVDFREIAPIRATRNMYLDDKGNVIDGKSLYTHSA
                     VGVPGTVAGMAHAAQKWGTMPLAKLIQPSIELADKGFPVSETLAKVLGQETKNMGKWG
                     ATKAIYWKNGAPLKPGDMLVNKDLAQSMRLIAKDGAKAFYEGAIAQKIVADMAKNNGP
                     ITAQDLREYKVVEREPVRGSYRGYQIVTMPPPSSGGAHLVQILNMMERWPMKDWGHNS
                     ANSIHHVSEAMKLAYADRSEYLGDPDFVKIPLKGLTSKKYADSLAQLIDPNRARIGKE
                     IKPGKPQPYESDQTTHYSVMDKAGNIVAVTYTLNTNFGSGIVAPGTGIMLNNEMDDFS
                     AKPGVPNAYGLIGGDANAVGPKKRPLSSMTPTFVLKDGKPWLVTGSPGGARIITTVLQ
                     QVVNAIDYGMNPAEAASALRFHQQWTPDELRIEKGFSPDTIAILKQRGQNVAVKASMG
                     RTQTIQRQGNEFWGYSDPRNPDGKTLGY"
     gene            complement(9378..11171)
                     /locus_tag="PKV30_03035"
     CDS             complement(9378..11171)
                     /locus_tag="PKV30_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRL29227.1"
                     /translation="MPESVARSAAKSPRALRGLLPFLRPYRVRMALALLFLVGAAAAT
                     LALPLALRSLIDGGLVSADPGARVMALREHFVALFGVGVALGVFSALRFYMVSWLGER
                     ITADLRNAVYAHVVRQSPEFFETTQTGEVLSRLTTDTTLVQTVVGSSLSMGLRNMVMG
                     VGALAMLVITNPRVMLPVMGMLALIVLPSVYVGRRVRRLSRASQDRVADASAIAAEVL
                     NAIPVVQSYTQQRRESARFEAATERAFDTAVRRTRVRSVLVAFIISATFGALLWGLYQ
                     GTQAVLRGDITAGHLGQTVLYVIVLVSSVAVLSEVYGEVLRAAGATERLMELLHARSP
                     VDEPARPVVLPAAQGGSSVRFDHVTFHYPSRPAQAALRDFSLDVRPGETVALVGPSGA
                     GKSTVLQLLLRFYDAQQGVVSVDGVPVNAVPLDELRGRIGIVPQDSTVFSASAMDNIR
                     YGRPDAGDDEVFAAARAAFADDFICALPEGYATHLGERGVRLSGGQRQRISIARAMLK
                     NPPLLLLDEATSALDAESERMVQAALEAAMQGRTTVVIAHRLATVLRADRIVVMEGGR
                     IVDEGTHAELVARGGLYARLAAMQFGLEELS"
     gene            complement(11305..11718)
                     /locus_tag="PKV30_03040"
     CDS             complement(11305..11718)
                     /locus_tag="PKV30_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRL29228.1"
                     /translation="MSQNKPVPAPERVVLRRRLAVLMWRAVAVLGLTLGLLGVVLPVV
                     PTVPFLILSAWAASKGWPAFEIWLLNHRLFGPPIVQWRERGAVPRRAKWFSTLMMAAS
                     GIGMQFFTGIPLWLRVAVPAVMLAVAIWLWLRPEQ"
     assembly_gap    11807..11906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12139..>13151
                     /locus_tag="PKV30_03045"
     CDS             12139..>13151
                     /locus_tag="PKV30_03045"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRL29229.1"
                     /translation="MISLPHFQPPAPFTDADAALAQVRRIYDAGCAHLRNALAAYIAG
                     PAEPQGPLIRVRAFYPFVRLHVPHGRHPDTRLAYGFVSHAGHYETTLTRPDLFADYDL
                     EQFRQLLANHGATLEVGTSSKPIPLPFALAEDEHFEGSLSAAQRQCLPDVFDLPDLTA
                     MDDRIANGTHTTAPGDPEPLALFTAPRVDYSLHRLRHYTGTSPEHFQNFVLFTNYQFY
                     IDEFIALGQLEMQNADSPYSAFVQPGNVITRRAGLGGGDEGGTPPPRLPQMPAYHLVR
                     PDRSGISFVNIGVGPSNAKTITDHIAVLRPHAWIMVGHCAGLRNSQQLGDYVLAHGYV
                     RED"
BASE COUNT         2481 a         4482 c         4078 g         2010 t
ORIGIN      
        1 cccgctatgt catgaacaag ccgcacgcct ggatgctgga cgcgcagatc atgctttacg
       61 gcacgctgtt catgaccgcc ggcgcctaca cgctgtcgaa gaacggccac gtgcgtggcg
      121 acgtgctgta cggctcttca agccgcgcac gcaggccacc atcgacctgc tgctctacat
      181 cgtgttcttc ctgcccggca tcgtggcgct gacctgggcc ggctggagct atgcgcagga
      241 atcgctggcg attcgcgagc agaccttcaa cgccgacccg ctgccggtct acccgttcaa
      301 gttcgtcatt ccgctgtcgg gcggcatcct gctgctgcaa ggcatcgtcg aaatcatccg
      361 ctgcgtcatc tgcctgcgca ctggcgcctg gccagcgcgc gacgaggacg tggaagaagt
      421 cgacgtcgac aagctcaagg agatggtgca cgtgaaggac gaagacattc aggcgctgga
      481 ccagtatgtt gtggcgcagc aggagcacaa ggcatgaagc tgcgcaagga actgtggttc
      541 ggcttcacgc ggatggcgct gatcatcgcc gccgcgctgg gcatgctggt cagcgtcgag
      601 aagatcacca acggccacgt cggcctgctc atgctgtcgc tggtggtggt ggcgatcatg
      661 ctgggcttcc ccaccgcctt cacgctgatg ggcatgggca tgctgttcac ctggctggcc
      721 tacgaccgcg actggcaccg cacgctggac ctgatggtgc agtcggcctt caaggtgatg
      781 ggcaacgacg tgctgattgc cgtgccgctg ttcgtcttca tgggctatct ggtcgagcgt
      841 gccaacctga tccagaagct gtttcacagc ctgcacctgt cgatggcgcg cgtgccgggg
      901 tcgctggcgg tggccacgct ggtcacctgc gcggtgtttg ccaccgccac cggcatcgtc
      961 ggcgccgtgg tcaccttgat gggcctgctg gcgctgccgg ccatgctgcg cgccggctac
     1021 agcgtgccgc tgtcggccgg cgccatcacg gcgggcggct gcctgggcat cctgattccg
     1081 ccttcggtgc tgctgatcgt gtacggcgcc acggcgggcg tgtcggtggt gcagttgtac
     1141 gcgggcgcgt tcttccccgg cctcatgctg gtcggcctgt acgtgctgta cgtcatcatc
     1201 gtcgccaagc tcaagcccgc ctgggcgccg ccgctgagcg ccagcgatcg gctggtggcg
     1261 ctggcggcgc cattggtcag cctgggcgcg cgcaacgaac agggcgccat gggcttcatg
     1321 ctgcgcacgc tgcgccgagg cgattcggcg gcggaaaaaa actatctgcg caagcaattg
     1381 ttcgtcgcgc tggtgcccgc cctgctgttt gccgcgatgg cgttctggag ttaccagctt
     1441 ttcacggcgg aagagcgcgt ggatgaagtg cccgccgagc ttgagcgcat gggcgctggc
     1501 ggtgaagcga ccagcagcca gggtggtctg gccgagccac ccggcagcgg cgaatccgct
     1561 gcgccatccg gcagcggcct ggcggagcca cccaccgagc cgtcgccccc tgcggaaggt
     1621 gggttggccg agcccccggg cagcagcgac accccggccg ccgcgagcga cgccagcggt
     1681 ctggcggaaa ccccaggcgc cgcccccgcc gcggatgcgc ccaccaccgc cggcagcctg
     1741 gccgagcctc ctgcggttgg cgcgcagccc ggcggcgcca gcgcgacgcc gacaccgcag
     1801 accagcgccg ccgaggcccc cagcgcccca cgcggctact ggatcaccgt cggcttgggc
     1861 gcgctcgcgc tgctggcgta ctacgcattg ctcaacttca accggctgga aatcttcaag
     1921 atgctgctgt ccagcttctt cccgctggtg atcctgatcc tgagcgtgct gggctccatc
     1981 gtgttcggac tggcaacgcc gaccgaggcg gcggccgtgg gcgcattcgg tggctacgtg
     2041 ctggcttgca tctacgcgct cgtcggcaaa cgcaagggcc gcgtcggtgc cacgctggcc
     2101 ttgtggctgc tgctgtggtt gccggcgctg gtggcgattg gctggtttct gctcggccgc
     2161 ggggaatcga acgcgtcgcc ggtgccgcct tggctgggct ggctggccgt ggcggggatc
     2221 ggcgtctggt gcgtggtggg ctatcggcag gctggcctgc aaggcgaagt caaggaaagc
     2281 gtctacctca cggccaagac gtcggccatg gtgtgctggc tgttcgtcgg ctcggccatt
     2341 ttctcggccg ccttcgcctt gctgggcggc caggaactga tggaacagtg ggtgctgggc
     2401 atgaacctca gcaagaccca gttcctgatc ctgacgcagg tcatcatctt catcctgggc
     2461 tggccgctcg agtggaccga gatcatcgtc atcttcatgc ccatcttcgt gccgctgctg
     2521 gacaactttg gcgtcgaccc gctgtttttc ggcctgctgg tggcgctcaa cctgcagacg
     2581 gcgttcctca gtccgccagt ggccatggcg gcgttctatc tcaaaggcgt cagtccgcca
     2641 cacgtcacgc tgaaccagat cttcctgggc atgctgccct tcatgggcat ccagattctg
     2701 gccatcgtgc tgctgtacgt gtttccgcag atcggcctgt ggctgccgca aatcctgtac
     2761 aaataggctt cgccgcctaa ggccgaacat caggccagcc gcgcgatcgg ctggcctttt
     2821 ttcatggcaa cccgcataat atgacgttta cgtaaacgtc aatcaaatct ggagacgccc
     2881 gcatggcgaa gaagaccgat gctcaggcaa gccacgacgc tgcgcgctgg caggtgccaa
     2941 ttggcggcaa gggcttcgat ctgaaagatt gcgaccccgc ggccaagccc tgttccagcg
     3001 gcgacaagac agccgacaag acgcgcgtga ccgatctggc gcgcgagatt gatgcgctgc
     3061 aggacgtgtt ctgggcggac aaacgcttca agctgctggt gctgctgcaa ggcaccgaca
     3121 ccgccggcaa ggacggcacc atccgcgccg tgttcaagga aaccagcccg ctgggcgtgc
     3181 gcgtcgtcgg ctggaaggcg cccagcgaac tggagcgcag ccacgactac ctgtggcgca
     3241 tccaccatga gatgccggcg tcgggcgaat tcatgatcca caaccgcagc cactacgagg
     3301 acgtgctggt gccggtggtc gagcgctgga tcgacaagaa aaccaccgag cagcgctacc
     3361 gccagatcaa cgacttcgag cgcatgctga gcgagaacgg caccgtcatc atgaagttca
     3421 tgctgcacat cggctttgac gagcagcgcc agcgcctgca agagcgcatc gacgacccgg
     3481 ccaagcactg gaagttcagc cttggcgacc tggacgcgcg caagcaatgg ccgttgtacc
     3541 aacaggcgta cgaccatctg ttgcgcgcca ccagcaccga atgggcgccc tggaccgtgg
     3601 tgccggccga cagcaagacg caccgcaacc tgatgatcgc cacgctggtg cgcgacaagc
     3661 tgaagtcgct caagctgcgt tacccgcccg acaatccaga gctgaaaggc ctgcgcgtgc
     3721 agtgaaatca agcgcaagaa gctatcaaaa acatagtaga caaacatgac cgacctgtcc
     3781 gccgagttca aggccctggc cgaataccgc cccacgaaca aggtgcgctt cgtcaccgcc
     3841 gcctcgttgt tcgacggcca cgacgcggcc atcaacatca tgcggcgcat cctgcaaggc
     3901 atgggcgccg aggtgattca ccttggccac aaccgcagcg tcgatgaggt ggtgaccgcc
     3961 gccttgcagg aagacgcgca gggcatcgcc atcagcagct accagggtgg gcatgtcgag
     4021 tatttcaagt acatggtcga tctgctgaac agccgcggcg gtgcgcacgt gcaggtgttt
     4081 ggcggcggtg gcggcgtgat cgtgccggcc gaaatccgcg agctgcacga ctatggcgtg
     4141 gcccgcatct acagccccga agacggccag cgcatgggcc tgcaagggat gatcggcgag
     4201 atggtgatgc gttgcgatca ggatttgtcg ggctacgccc cgaccgccat cgaggcgatt
     4261 caaggccatg gcgaagctgc ctggcgcgcg ctggcgcagc tgatcacggc actggagaat
     4321 gaaaaggccg atgaggcgct ggttgccgcc gttcgtgcgc aggcagctac caaaaagata
     4381 cctgtggtcg gcatcaccgg cacagggggc gcgggcaaat caagcctgac cgacgagctg
     4441 atccgccgcc tgcggctgga ccaggacgac gcgttgcgcg tggcggtgat cagcattgac
     4501 ccatcgcggc gcaagagcgg cggcgcactg ctgggcgacc gcatccgcat gaacgccatc
     4561 gggccttgga ctcaacccac cgttcgccct gagcctgtcg aagtgcaggc cgagccttcg
     4621 acaagctcag cccgaacgga tttgggacaa cgcgtctaca tgcgctcgct cgccacgcgc
     4681 gacaccggca gcgagatcag cccggcgctg cccgacgtca tcgccgcctg caaggcggcc
     4741 gggttcgacc tgatcatcgt cgagacctct ggcatcggcc agggcgacgc cgccatcgtg
     4801 ccgcacgtcg atgtgcccat gtacgtgatg acgcccgaat acggcgccgc cagccagctt
     4861 gaaaaaatcg acatgctgga ctttgccgag ttcatcgcca tcaacaagtt cgaccgcaag
     4921 ggcgcgcagg acgcgctgcg cgacgtggcc aagcaggtgc agcgcaataa gagcgcgttc
     4981 aatgtgcggc ccgaagaaat gccggtgttc ggcacccagg ccgcgcgctt caacgacgat
     5041 ggggtgacgg cgctgtacca ggcgttgaaa gtgcgcctcg gtgaattggg cctgccgctc
     5101 aaagaaggcc gcctgccgcc cgtgaacgtg cgccacagca ccaaccagac gcccatcgtg
     5161 ccgccggcgc gcacccgcta cctggcggaa attgccgaca ccgtgcgcgg ttacaagcag
     5221 cgcgtgcgcg cacaggcgcg gctggtgcgc gaggtgcagc agttgcgcga aacggcgcgc
     5281 atgctgcaag ccgacaaccc ggacaagacc ggcgaacacg gcgcgcgcgc caccgtgctg
     5341 aaactggccg acgagcgcgc cacgcgcgtc gaccgcgacg ccgccaagct gatcgagcag
     5401 tggccggcga tgcagcaagc gtatgcgggc gacgaatacg tggtgaagat ccgcgacaag
     5461 gaaatccgca ccgcgctcac caccaagtca ttgagcggca ccaccatccg caaggtcgtg
     5521 ctgccgcagt tcgaggacca cggcgaaatc gtcaagtggc tgatgctgga caacgtgccc
     5581 ggcagctacc cctacaccgc cggcaccttt gccttcaagc gcgagggcga agacccgacg
     5641 cgcatgttcg cgggcgaggg cgatgccttc cgcaccaacc ggcgcttcaa attggtgagc
     5701 gagggcatgc cggccaagcg gctctcgacc gcgttcgatt cggtcacgct ctacggaaac
     5761 gaccccgatc cacggccgga catctacggc aaggtcggca actcgggcgt cagcatcgcc
     5821 acgctggacg atttgaaggt gctgtattca gggttcgacc tgtgcaaccc gaccaccagc
     5881 gtcagcatga cgatcaacgg cccggcgcca tcgatcctgg ccatgttcat gaacgcggcc
     5941 atggatcagc aactggagaa attccaggcc gacaacggcc gtcagccgac cgacaccgag
     6001 gccgaaaaaa tcaaggaatg gacgctggcc aacgtgcgcg gcacggtgca ggccgacatc
     6061 ctgaaagaag accagggcca gaacacctgc atcttctcga ccgagttttc gctcaaggtc
     6121 atgggcgaca tcgccgaata cttcgtgcac cacaacgtgc gcaacttcta cagcgtgtcg
     6181 atcagcggct accacattgc cgaagcgggc gcgaacccga tcagccagtt ggccttcacg
     6241 ctcagcaacg gcttcacgct ggtcgaagcg tacctggcgc gcggcatgca catcgacgat
     6301 ttcgcgccca acctgagcta tttcttctcg aacggcatgg acccggaata caccgtgatg
     6361 ggccgcgtgg cgcgccgcat ctgggcggtg gcgatgaagg aaaagtacgg cgccaacgag
     6421 cggtcgcaaa agttgaaata ccacatccag accagcggcc gcagcctgca cgcgcaggag
     6481 atccagttca acgacatccg caccacgctg caggcgctga tcgccatcta cgacaactgc
     6541 aacagcctgc acaccaacgc gtttgacgag gccatcacca cgccgaccga agacagcgtg
     6601 cgccgcgcga tggcgatcca gctcatcatc aaccgcgaat ggggcctggc caagaacgag
     6661 aacccgaacc agggcgcctt tgtgatcgag gagttgaccg agctggtcga agaagcggtg
     6721 ctgagcgagt tcgaaaagat cgccgaccgc ggcggcgtgc tgggcgcgat ggaaaccggc
     6781 taccagcgca gcaagattca ggaagagagc atgcattacg agatgctcaa gcacaccggc
     6841 gagtacccca tcatcggcgt caacaccttc cgcaacccca agggcgacga ggtgctggaa
     6901 agcctggaac tggcgcgctc gaccgaggac gaaaagcgat cgcagcttgc gcgcctggcc
     6961 gacttccacc agcgccatgc gggcgaagcg cccgccatgc tggcgcgcct gcagcaggcc
     7021 gtgatcgaca acggcaacgt gttcgcggtg ttgatggacg cggtgcgcgt gtgctcgctg
     7081 ggccagatca ccaacgcgct gttcgaggtg ggcggtcagt atcggcgtag catgtagccc
     7141 gctgtgcccg agcgcggctc actacgttat tcatagctga tcgcgctttc tggacgagcg
     7201 ctggagtccg atttcattca tgaaatcggc cgtgtcgatc acaacaagag gagcctcaac
     7261 atgaccctgc aaccccgact gctgctgggc agcatcgcct tggccgtgct caccgcgtgc
     7321 agcaccgcca ccgcgccacc ggcgcaggcc ccggccgcca ccgcgcccac caccacatcg
     7381 gccacgcaag ccgccgctgc cgcttacgac tgggacatgg acgtgtttca ccccgtcgtc
     7441 gcgcgaaacg gcatggtcgc cagcgagcag gagctggcct cacagatcgg tctgaacgtg
     7501 ttgaagtccg gcggcaacgc ggtcgatgcg gcggtggcca tgggctttgc gctggccgtg
     7561 gcgctgccca acgccggcaa tctgggcggc ggcggtttca tgatggtgca cgacgccaag
     7621 accggcaagg acgtggcggt cgacttccgc gaaatcgcgc cgatcagggc cacgcgcaac
     7681 atgtacctgg acgacaaggg caacgtgatc gacggcaagt cgctctacac ccactcggcc
     7741 gtcggcgtgc ccggcacggt ggccggcatg gcgcacgcgg cgcagaagtg gggcacgatg
     7801 ccgctggcca agttgatcca gccctcgatc gagctggccg acaagggctt tcccgtcagc
     7861 gagacgctgg ccaaggtgct gggccaggaa accaagaaca tgggcaagtg gggcgccacc
     7921 aaggccatct actggaagaa cggcgcgccg ctgaagcccg gcgatatgct ggtcaacaag
     7981 gatttggcgc agtcgatgcg cctgatcgcc aaggacggcg ccaaggcctt ttatgaaggc
     8041 gccatcgcgc agaaaatcgt ggccgacatg gcaaaaaaca acggccccat caccgcgcag
     8101 gatttgcgcg aatacaaggt ggtcgagcgc gaaccggtgc gcggcagcta tcgcggctat
     8161 cagatcgtga ccatgccgcc gccttcgtcc ggcggcgcgc acctggtgca gatcctgaac
     8221 atgatggagc gctggcccat gaaggactgg ggccacaaca gtgccaacag catccaccac
     8281 gtgtccgagg ccatgaagct ggcgtatgcc gaccgcagcg aatacctggg cgaccccgat
     8341 ttcgtgaaga tcccgttgaa aggcctgacc agcaagaagt acgccgattc gctggcgcaa
     8401 ctgatcgacc cgaaccgtgc tcgcatcggc aaggagatca aacccggcaa gccgcagccg
     8461 tacgagagcg accagaccac gcactactcg gtgatggaca aggccggcaa catcgtcgcc
     8521 gtgacctaca cgctgaacac caactttggc tcaggcatcg tggcgcccgg caccggcatc
     8581 atgctgaaca acgagatgga cgacttcagc gccaagcccg gcgtgcccaa cgcctacggc
     8641 ctgatcggcg gcgacgccaa cgccgtcggc ccgaagaagc gcccgttgtc gtcgatgacg
     8701 cccaccttcg tgctgaaaga cggcaagccg tggctggtca ccggcagccc tggtggcgcg
     8761 cgcatcatca ccacggtgct gcagcaggtg gtcaacgcga ttgactacgg catgaacccg
     8821 gccgaagccg catcggcgct gcgctttcac cagcagtgga cgcccgatga gttgcgcatc
     8881 gaaaagggct tttcgcccga caccatcgcc atcctgaagc agcgcggcca gaacgtggcc
     8941 gtcaaggcat cgatgggccg cacgcaaacc atccagcgcc agggcaatga gttctggggc
     9001 tactccgacc cgcgcaaccc ggacgggaaa acgctgggct attgatttaa tagctgcttg
     9061 cgcttgaagc ccgcgcgcca gcgggctttt ttacgggcaa tcgtctatca aacgccgcgt
     9121 cctcacccct gccctctccc agaggcagag ggagaaatac cgaagccgga gcgaagcagc
     9181 ggcgttgacg aaacccaggc cgagcgcagc gatggcccgt gtggattccc cctcccctct
     9241 ggccgtgccg agaagcgcag agcgtggcgg gcgcgcagcc gccttttctt cggttacttt
     9301 cttttggcgg agcaaaagaa agtgactgct ccgccgggcg cacatccccg ccgacagcgc
     9361 caaacaagcg caccgcctca gctcaactcc tcaagcccaa actgcatcgc cgccaaccgc
     9421 gcatacaacc caccccgcgc caccaactcg gcgtgggtgc cctcatccac aatgcggccg
     9481 ccctccatca ccacaatccg atccgcccgc aacaccgtcg ccagtcggtg cgcgatcacg
     9541 actgtcgtgc gcccctgcat cgccgcctcc agcgccgcct gcaccatgcg ctcgctctcc
     9601 gcatccagcg cgctggtcgc ctcgtccagc aacaacagcg gcgggttctt cagcatcgcc
     9661 cgcgcaatgc tgatgcgctg acgctgtccg ccagacaagc gcaccccgcg ctcgcccaaa
     9721 tgcgtcgcat agccctccgg cagcgcgcag atgaagtcat ccgcaaacgc cgcacgtgcc
     9781 gctgcgaaca cctcatcgtc gccagcatca ggtcgcccat agcggatgtt gtccatcgcc
     9841 gaggccgaga acaccgtact gtcttgcggc acgatgccga tgcgcccgcg cagttcatcc
     9901 agcggcaccg cattcaccgg caccccatcc acgctgacca cgccttgctg cgcgtcgtaa
     9961 aagcgcagca gcagttgcag caccgtgctc ttgcccgcgc cgctggggcc gaccagcgcc
    10021 accgtttcgc ccggccgcac gtcgagtgaa aaatcacgca gcgccgcctg cgccgggcgc
    10081 gacgggtaat gaaaagtgac gtgatcgaag cgcaccgatg agccgccttg cgccgccggc
    10141 agcaccaccg gccgcgccgg ctcatcgacc ggcgagcgcg catgcagcag ttccatcagc
    10201 cgctcggtcg cgccggcggc gcgcagcacc tcgccataca cctccgacag caccgccacc
    10261 gagctgacca gcacgatcac gtacagcacc gtctgcccca ggtggccggc cgtgatgtcg
    10321 ccgcgcagca ccgcctgcgt gccttggtac aagccccaca gcagcgcgcc aaacgtcgcg
    10381 ctgatgatga acgccaccag caccgaacgc acgcgcgtgc gccgcacggc ggtgtcgaac
    10441 gcgcgctcgg tcgccgcctc aaagcgcgcc gattcgcgcc gctgctgcgt gtagctttgc
    10501 accacgggaa tggcgttcag cacctcggcg gcgatggcgc tggcgtcggc cacgcggtcc
    10561 tggctggcgc gcgacaggcg ccgcacgcgc cggcccacgt acacgctcgg caacacgatc
    10621 agcgccagca tgcccatcac cggcagcatc acgcgcgggt tggtgatgac cagcatggcc
    10681 agcgcaccca cgcccatcac catgttgcgc agccccatgc tgagcgacga gccgaccacc
    10741 gtctgcacca gcgtggtgtc ggtggtcagg cgactcagca cttcgccggt ctgtgtggtt
    10801 tcgaagaact ccgggctttg ccgcaccacg tgcgcataca ccgcgttgcg caagtcggcc
    10861 gtgatgcgct cgcccagcca gctcaccatg taaaagcgca gtgcggagaa cacgcccagc
    10921 gccacgccca cgccgaacag cgccacgaag tgctcgcgca atgccatcac ccgcgcgccc
    10981 gggtcggcgc tcaccagtcc gccgtcgatc agtgagcgca gcgccagcgg caaagccagc
    11041 gtggccgccg ccgcgccgac cagaaacagc agcgccaggg ccatgcgcac gcggtaagga
    11101 cgcagaaaag gcagcagccc gcgcaaggcg cgcgggcttt tggcggcgga gcgagcaaca
    11161 gattcaggca tcaaaagaat cggcgttcgc agtcacaaaa aaacaaaggg ccgcagagca
    11221 ggccattcgg aaacgccgcg ctccggttct ccccgagcgc cagcgttgtc ccctggggga
    11281 aggcgccgca ggcgccacag ggggtcattg ttcaggtcgc agccacaacc agatcgccac
    11341 cgccagcatc accgccggca cggcgacgcg cagccacagc ggaataccgg tgaaaaactg
    11401 catgccaatg ccgctggcgg ccatcatcag cgtgctgaac catttggcgc gacgtggcac
    11461 ggcgccgcgc tcgcgccatt gcacgatcgg cggcccgaac agacggtggt tcagcagcca
    11521 gatctcaaac gcgggccagc ccttgctcgc cgcccaggcc gacagaatga gaaacggcac
    11581 cgtgggcacc acgggcagca ccacgcccag cagccccagc gtcagaccca gcaccgccac
    11641 cgcgcgccac atcagcaccg ccaagcgccg gcgcagcacc acgcgctccg gcgcggggac
    11701 aggtttgttc tgggacatgg gaatcaaatc gggttctggc gcatgctcag acggcgccag
    11761 cacccatcat attgtcgtgc cggcgatgcg ccgtaggacg cactacnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnncggc gccagcaccc atcatattgt cgtgccggcg
    11941 atgcgccgta ggacgcacta cccaagccac gccgtcctag ggctgagcgt tgtgccacct
    12001 ggtttgcaac gatttttata gctgcttgcg cttgttctgc aagcgctggc ggccgatttg
    12061 attcacaaat ctccgtccag caccgcagcc acccgccgcc cgtcacacgc aggcgatgcg
    12121 cgccgtggac aatggctcat gatctcgctg ccccactttc agccccccgc gcccttcacc
    12181 gatgccgacg ccgcgctggc ccaggtgcgc cgcatctacg acgccggctg cgcccacctg
    12241 cgcaacgcgc tggcggccta catcgcgggg ccagccgaac cccagggccc gctgatccgc
    12301 gtgcgcgcct tctatccctt tgtgcgcctg cacgtgccgc acgggcgcca ccccgacacg
    12361 cgcctggcct acggctttgt ctcgcacgcc ggccactacg aaaccaccct gacacgcccc
    12421 gacctgtttg ccgactacga tctggaacag ttccgccaat tgctcgcgaa ccacggcgcc
    12481 acgctcgaag tgggcaccag cagcaagccg attccgctgc ccttcgcact ggcggaggac
    12541 gaacactttg aaggatcgct cagcgccgcg cagcgccaat gcctgcctga tgtgttcgac
    12601 ctgcccgacc tgacggcgat ggacgaccgc atcgccaacg gcacgcacac cacggcgccg
    12661 ggtgaccccg agccgctggc gctgttcacc gcgccgcgcg tcgactactc gctgcaccgc
    12721 ctgcgccact acaccggcac ctcgcccgag cattttcaga acttcgtgct gttcaccaac
    12781 taccagttct acatcgacga gttcatcgcg ctcggccagt tggaaatgca gaacgcggac
    12841 agcccctaca gcgccttcgt gcaaccgggc aacgtcatca cgcgccgcgc cgggctgggc
    12901 ggcggcgacg aaggtgggac gccgccgccg cgcctgccgc aaatgccggc gtatcacctg
    12961 gtgcggccgg accgcagcgg catcagcttc gtcaacatcg gcgtcggccc cagcaacgcc
    13021 aagaccatca ccgaccacat cgccgtgctg cgcccgcacg cgtggatcat ggtcggccac
    13081 tgcgccggct tgcgcaacag ccagcaactg ggcgactacg tgctggcgca cggctacgtg
    13141 cgcgaagacc a
//
LOCUS       DAOMVO010000032        13090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1080_length_13090_cov_15.920368, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000032 DAOMVO010000000
VERSION     DAOMVO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_13090_cov_15.920368"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PKV30_03050"
     CDS             <1..1418
                     /locus_tag="PKV30_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRL29230.1"
                     /translation="QTGGMTRDIERGVRGIESLVSFSLYSIVPTLVEVAMVLTILAVK
                     FDARFAWITGGALVLYIAFTVVVTEWRTQFRRQANEADSSAHTKAIDSLLNYETVKYF
                     NNEGFEARRYDESLEALRRARLKSQTTLSLLNAGQQLIIAVGLVAMLWMATQGVVDGR
                     LTLGDLVMVNAFMIQLYIPLNFLGVIYREIKQNLTDLDKMFTLMDREREVADAPGAEA
                     LRTNGPVDVRFEHVDFAYESARAILHDVSFEIPAGKTVAVVGPSGSGKSTLARLLYRF
                     YDVQAGKILIAGQDIRTVTQSSVRRAIGIVPQDTVLFNDTVAYNIRYGKPDASDAEVE
                     QAARAAHIHDFIMSTPKGYATMVGERGLKLSGGEKQRVAIARTLLKDPPILIFDEATS
                     ALDSANERAIQAEIKSAARGKTTLVIAHRLSTVVDAHEILVMELGRIIERGTHLQLLA
                     ARGRYADMWALQQSAEQHAPT"
     gene            1568..2479
                     /locus_tag="PKV30_03055"
     CDS             1568..2479
                     /locus_tag="PKV30_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRL29231.1"
                     /translation="MKKQLLALAVLALAAGTASAQANDTLAKIKSSGTVNLGVRDSSG
                     LAFTIGGGKYVGFHTEMAEHIVEDLKKVTGNPNLKINYQVITSQNRIPLVQNGTIDFE
                     CGSTTNNAGRQKDAAFAVTTFVDEVRTAVKANSGIKSVADLNGKTVVTTTGTTSVQHL
                     RRHERGANIDFKTVQGKDHADSFLLLESGRADAFVMDGSILAANIARAKSPKDFAIVG
                     EALSFEPIACMLRKDDPKMKAALDNSIKRQIKDGSLAKLYDKWFMQPIPPTNTPLNMP
                     MNESTKAAWANPNDKPAEAYTSKLLSK"
     gene            2578..3336
                     /locus_tag="PKV30_03060"
     CDS             2578..3336
                     /locus_tag="PKV30_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL29232.1"
                     /translation="MNMEWQVFCKDTLTGDVVASCLGTAKDPTYLNWIFKAWGWTVGV
                     SLTALVVALAVGSIIGVIRTLPDSPWLTRFGNAWVELFRNIPLLVQIFLWYFVLPALI
                     PPLKSLPPFVLVVLALGFFTSARIAEQVRAGIQALPKGQRYAGMALGFTTPQYYRYVI
                     LPMAYRIIIPPLTSESMNIFKNSAVAFAVSIPELTQFALQAGEETSRPIEIYLGVTIL
                     YVISALVINRIMAFIEKRARVPGFVASGTGAGGH"
     gene            3338..4006
                     /locus_tag="PKV30_03065"
     CDS             3338..4006
                     /locus_tag="PKV30_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL29233.1"
                     /translation="MLNLDFSILNWNLIQGFILKGLWFSITLTVVATLGGIFFGTILA
                     LMRLSGKKWLDVPAAIYVNGMRSIPLVMVILWFFLLVPFIIGRPIGAEYSAFITFIAF
                     EAAYFSEIMRAGIQSIPRGQVFAGQAMGMTYGQNMKLVILPQAFRNMLPVLLTQTIIL
                     FQDTSLVYAIGAYDLLKGFETAGKNFGRPIEMYLTAAVLYFIICFSLSYVVKRLHKKI
                     AIIR"
     gene            4029..4766
                     /locus_tag="PKV30_03070"
     CDS             4029..4766
                     /locus_tag="PKV30_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRL29234.1"
                     /translation="MIQVKNVSKWYGPVQVLNDCSVNIDKGDVVVVCGPSGSGKSTLI
                     KTVNGLEPIQKGDIVVNGISLTDPKTDLPKLRSKVGMVFQHFELFPHLSVTENLTIAQ
                     IKVLGRSADEAKTRGLKMLDRVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMVGLAKEGMTMMVVTHEMGFARKVANRVIFIDVGGRILED
                     CSKDEFFGHPENRQPRTKDFLNKILQH"
     gene            complement(4963..5370)
                     /locus_tag="PKV30_03075"
     CDS             complement(4963..5370)
                     /locus_tag="PKV30_03075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL29235.1"
                     /translation="MLAPDQLNDLLAPLFPGLMGVRVLQASPDEVRAEMLVRPDLCTA
                     GGILHGGAYMAFADTLGAVGTVMSLAKGQSTTTTHSSTKFIRGAPVGSTVTGHSVALH
                     RGRRTHVWQTEVRNAEGKLCAVVTQTQLVMDAA"
     gene            complement(5520..6560)
                     /locus_tag="PKV30_03080"
     CDS             complement(5520..6560)
                     /locus_tag="PKV30_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRL29236.1"
                     /translation="MKLRRTLLALAAATTALAGATFALPAAAQNYKSEYRMSLVVGPA
                     FPWGKGGEIWANKVREKTNGRINIKLYPGVSLIQGDQTREFSALRQGVIDMAVGSTIN
                     WSPQVKQLNLFSLPFLMPDYAAIDALTQGEVGKQMFSILDKAGVVPLAWGENGYRELT
                     NSKHVVKTPADLKGLKIRVVGSPLFLETFTALGANPTQMSWADAQPALASGAVDGQEN
                     PMSIFTAAKLQNVGQKYLTMWGYIADPLVFVVNKQVWESWTPADRELVRQAAIEAGKE
                     QIGIARKGMIEPGQPLLKDIAALGVTVTHLSPAERDAFVKATRPTYDKWKSTVGTDLV
                     SMAEKAIAARKK"
     gene            complement(6650..7948)
                     /locus_tag="PKV30_03085"
     CDS             complement(6650..7948)
                     /locus_tag="PKV30_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRL29237.1"
                     /translation="MIATLLFAVFIITMMLGVPIGASLGLAGAAAIALANAEAPWFGL
                     LAVPQNFYAGLGKYPLLAIPMFVLVGSIFDRSGVALRLVNFAIALVGRGPGMLPLVAI
                     CVAMFLGGISGSGPANAAAVGAVMIAAMNRAGYPASYSASVVGAAAATDILIPPSVAF
                     IVYSVLVPGASVPALFAAGMIPGVLAGIALIVPAVWMARKHGMGHLESSLPRPPLLKS
                     FIEAIWGLAAPVLILGGMRMGWFTPTEAAVVAVFYGLFVGLFVHRSMTLRDLFPILKE
                     SGELSAVILLVVSLAGIFAFSLSTLGVIDPITRAIVNSGLGEYGVLALLILMLITIGM
                     FLDGVSIFLIFVPLLWPIVQHYNWDPVWFGVLLTLKVALGQFTPPLAVNLMVSCRIAN
                     VRMEETVRWVGPMLLAMGLVMVAVIIWPELALWLPRVLGY"
     gene            complement(8034..8630)
                     /locus_tag="PKV30_03090"
     CDS             complement(8034..8630)
                     /locus_tag="PKV30_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRL29238.1"
                     /translation="MSLPHESAPNAAAPLPKEDSAPTRVPLAIEDWLTVIIMALLALI
                     TFANVLVRYFTNASFAWTEEVSVFLMIVLAMVAGSAAVARNRHIRIEYFADSGPEARQ
                     RALAKFGAAMVALLFGLLAVLSARVLWDDVQYGETSPGIGVPQWWYTMWLPILSVAIM
                     GRAIGLMRRGGRAAADAELPTSPEVRAEEHAPRVCRIS"
     gene            9085..9510
                     /locus_tag="PKV30_03095"
     CDS             9085..9510
                     /locus_tag="PKV30_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRL29239.1"
                     /translation="MSTATDLHRTITDLDHVRIFNLLRRQTAAGGAPEHARDLAELID
                     AAEQVRPQEIAPDVVTMLSKLRVADPATGAERSITLCYPPEADAAQGMISVLSPLGTS
                     LLGLRVGEVARWRGIDGKTAELVVRAIEFQPEASGDYTA"
     assembly_gap    9767..9866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10122..11165
                     /gene="pyrC"
                     /locus_tag="PKV30_03100"
     CDS             10122..11165
                     /gene="pyrC"
                     /locus_tag="PKV30_03100"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRL29240.1"
                     /translation="MSTAPQTLTITRPDDWHLHVRDGAALADVVPHTAAQFGRAIIMP
                     NLRPPVTTTAQALSYKERIAQAVPAQAAFEPLMTLYLTDNTPPDEIARAADAGIVALK
                     LYPAGATTNSDAGVTDIRKTYKVLEAMRRAGLKLLVHGEVTDPDIDLFDREAVFIDRV
                     MIPLRRDFPELKVVFEHITTRDAAHYVASADAHTAATITAHHLLYNRNAIFVGGIRPH
                     YYCLPVLKREVHRQALVQAATGGSSKFFLGTDSAPHPAQLKEHASGCAGCYTAHAALE
                     LYAAAFEAAGALDKLEGFASFHGPDFYGLPRNGGTVTLKRESWTPPDSYPFGGAEVKP
                     LAGGEALAWRLAA"
     gene            11174..11665
                     /locus_tag="PKV30_03105"
     CDS             11174..11665
                     /locus_tag="PKV30_03105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRL29241.1"
                     /translation="MTKPSLADFAVTTLDGQPLNLADLTGRVLLIVNTASECGFTPQY
                     AGLQALHERYGAQGLTVLGFPCNQFGAQEPGDAGQIGAFCQRNYGVGFPMMAKVDVNG
                     AHAAPLFRWLKEQAPGLLGSQAIKWNFTKFLIGRDGRVIRRYAPQDKPESLARDIEVA
                     LAA"
     gene            complement(11670..12872)
                     /locus_tag="PKV30_03110"
     CDS             complement(11670..12872)
                     /locus_tag="PKV30_03110"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HRL29242.1"
                     /translation="MATPLELLFDLSFVVAIALAASQLHHGVMEHHTGPALAGFVAAF
                     SAIWWAWMNYTWFASAYADDSAAFRLLTMLQTVGVLIFAAGVPGLFNGDFRAGVLDYA
                     VMRLALVVQWLLAARGDPACAATCRRFALGITLVQALWIARLWFPVEWLWPTFVLFMA
                     CELAVPIIAERRHRTPWHAHHIAERYSLLLIITLGEVVLGATNAVAGKWQSFGWTLNL
                     ALVGLGSTMLAFALWWMYFLLPSGDALHRHRERGFFWGYGHFFIFASAAAVGAGLEVV
                     ADVLEEGKQAAAGIADGGHALAPAGHAGNAAHGVSPLYAISIVALAQALFMFSLWVIY
                     TRTTRAEASQGGLALLCLACVALGPLAVWLGAALPWGLLLLSVGPAIAIVYNEHGRKH
                     CAEGFAVR"
BASE COUNT         2387 a         4424 c         4099 g         2080 t
ORIGIN      
        1 gccagaccgg cggcatgacg cgcgacatcg agcgcggcgt gcgcggcatc gaatcgctgg
       61 tgtcgttctc gctgtattcc atcgtgccga cgctggtgga ggtggcgatg gtgctgacca
      121 ttctggccgt gaagttcgac gcccgctttg cctggatcac cggtggcgcg ctggtgctgt
      181 acatcgcctt cacggtggtg gtgaccgagt ggcgcacgca atttcgccgc caggccaacg
      241 aagccgattc aagcgcccac accaaggcga tcgactcgct gctgaactac gagacggtca
      301 agtacttcaa caacgaaggc ttcgaggcgc gccgctacga cgaaagcctg gaggcgctgc
      361 gccgcgcgcg cctgaaaagc cagaccacgc tgtcgctgct caacgccggc cagcaactca
      421 tcatcgccgt cggcctggtc gccatgctgt ggatggccac gcaaggcgtg gtcgatggcc
      481 ggctgacgct gggcgatctg gtgatggtca acgccttcat gatccagctc tacatcccgc
      541 tcaactttct gggcgtgatc taccgcgaga tcaagcagaa cctgaccgac ctggacaaga
      601 tgttcacgct gatggaccgc gagcgcgagg tggccgacgc gccgggcgcc gaggcgctga
      661 gaaccaacgg gcccgtggat gttcgctttg agcacgtcga ctttgcgtac gagtctgcgc
      721 gagcgatcct gcacgacgtg agcttcgaga ttccggccgg caagacggtg gcggtggtgg
      781 ggccgtccgg ctcgggcaag tcaacactgg cccgcttgct gtaccgcttc tatgacgttc
      841 aggctggcaa gattttgatc gccggccagg acatccgcac cgtcacgcag tccagtgtgc
      901 gccgcgccat cggcatcgtt ccgcaagaca cggtgctgtt caacgacacc gtggcctaca
      961 acatccgcta cggcaagccg gacgccagcg acgccgaggt cgaacaggcc gcgcgcgccg
     1021 cgcacatcca cgacttcatc atgagcacgc ccaagggcta cgccaccatg gtgggcgagc
     1081 gcggcctgaa gctgagtggt ggcgagaagc agcgcgtggc gatcgcccgc acgctgttga
     1141 aagacccgcc catcctcatc ttcgacgaag ccaccagcgc gctcgattcg gccaacgagc
     1201 gcgccatcca ggccgagatc aaaagcgccg cgcgcggcaa gaccacgctg gtcatcgcgc
     1261 accggttgtc caccgtggtc gatgcgcacg aaattctggt gatggagctg ggccgcatca
     1321 tcgagcgcgg cacgcacctg cagttgctgg ccgcgcgcgg ccgctacgcg gacatgtggg
     1381 cgctgcagca aagtgcggag cagcacgcac caacgtaaat gctggcccag tgccacgcca
     1441 agctggcacc acaattgcat gcggattgct agggtaaccc cgtagacagc ggtccgtaga
     1501 atgccgttga taaagcctcg ccctaccggt ctggcggggc ttcattgatc acttgaaagg
     1561 aacgtggatg aaaaagcagc ttctcgctct ggccgtactc gcgcttgccg ccggcacggc
     1621 atcggcacag gccaacgaca ccctggccaa gatcaagtcc agcggcaccg tcaatttggg
     1681 cgtgcgcgat tcctcgggcc tggcgttcac catcggtggc ggcaaatacg tgggtttcca
     1741 caccgagatg gccgaacaca tcgttgaaga cctgaagaag gtcacgggca accccaacct
     1801 gaagatcaac tatcaggtca tcacctcgca aaaccgcatt ccgctggttc agaacggcac
     1861 catcgacttt gaatgcggat cgaccaccaa caacgccggt cgccagaagg atgccgcctt
     1921 cgcggtcacc acgttcgtgg acgaagtgcg cacggccgtc aaagccaact cgggcatcaa
     1981 atccgtggcc gacctgaacg gcaagacggt ggtcaccacc accggcacca ccagtgtgca
     2041 gcacctgcgc cggcacgagc gcggcgccaa catcgacttc aagacagtgc aaggcaagga
     2101 ccacgccgac agctttttgc tgctcgaatc cggccgtgcc gacgctttcg tgatggacgg
     2161 ctcgatcctg gcggccaaca tcgcgcgtgc caagagcccc aaggatttcg ccatcgtggg
     2221 cgaggcgctg tccttcgagc cgatcgcttg catgctgcgc aaggacgatc ccaagatgaa
     2281 ggcggccctc gacaattcga tcaagcgcca gatcaaggac ggctcgctgg ccaagctgta
     2341 cgacaagtgg ttcatgcagc cgattccgcc gaccaacaca ccgctgaaca tgccgatgaa
     2401 cgaaagcacc aaggccgctt gggccaaccc caacgacaag ccggccgagg cttacacctc
     2461 caagctgttg tccaagtaac gcctgtttgg cgcagctttt gaacagcccg tgcgcgccag
     2521 ctcacgggct gttctcgatt tgagaacgga caccatggcg gaggcagcag cgagatgatg
     2581 aacatggaat ggcaggtgtt ctgcaaggac accctgacgg gcgacgtggt ggccagttgt
     2641 ctgggcactg ccaaggatcc cacctatctg aactggatct tcaaggcctg gggctggacg
     2701 gtcggcgtgt cgctgacggc gttggtggtt gccttggcgg tgggttccat catcggcgtc
     2761 atccgcacgc tgcccgacag tccctggctt acgcgctttg gcaacgcctg ggtggagttg
     2821 tttcgcaaca tcccgctgct ggtgcagatt tttctgtggt acttcgtcct gccagcgctg
     2881 attccgccgc tcaagagcct gccacccttc gtgctggtgg tgctggcgct gggcttcttc
     2941 acctcggcgc gtattgccga gcaggtgcgt gccggtattc aggcgctgcc caagggccag
     3001 cgctacgcgg gcatggcgct gggcttcacc acgccgcagt actaccgcta cgtcatcctg
     3061 ccgatggcgt accgcatcat cattccgccg ctcaccagcg agtcgatgaa catcttcaag
     3121 aactcggcgg tcgccttcgc agtgtcgatt cccgagctga cgcagttcgc actgcaggcg
     3181 ggtgaggaaa cctcgcgccc catcgagatt tatctgggcg tcaccatcct ctacgtgatt
     3241 tcggcactgg tgatcaaccg catcatggcc ttcatcgaga agcgcgcgcg cgtaccaggc
     3301 ttcgtggcgt ccggcaccgg cgcgggaggt cactgacatg ctcaaccttg acttctccat
     3361 cctcaactgg aacctgatcc agggcttcat cctcaagggc ctgtggttca gcatcacgct
     3421 caccgtcgtg gccacgctgg gcggcatctt cttcggcacc atcctggcgc tgatgcggct
     3481 gtcgggcaag aagtggctcg acgtgccggc cgccatctac gtcaacggca tgcggagcat
     3541 tccgctggtg atggtgatcc tgtggttctt cctgctggtg cccttcatca tcggccggcc
     3601 gatcggcgcc gagtattcgg ccttcatcac cttcatcgcc ttcgaggcgg cgtacttcag
     3661 cgagatcatg cgcgccggca tccagtcgat cccgcgcggg caggtgtttg ccggccaggc
     3721 catgggcatg acgtacggcc agaacatgaa gctggtgatc ctgccgcagg ccttccgcaa
     3781 catgctgccg gtgctgctga cgcagaccat catcctgttt caggacacct ccctcgtgta
     3841 cgccatcggc gcctacgact tgctcaaggg cttcgagacc gccggcaaga acttcggccg
     3901 cccgatcgag atgtacctga cggccgccgt gctgtacttc atcatctgct tctcgctgtc
     3961 ctatgtggtc aagcggctgc acaaaaagat tgccatcatt cgctaaggag cccgccatgt
     4021 ccgacaacat gattcaagtg aagaacgtct ccaagtggta cggcccggtg caggtgctga
     4081 acgactgctc ggtcaacatc gacaagggcg acgtggtggt ggtctgcggc ccgtcgggct
     4141 cgggcaagtc caccctcatc aagaccgtga acgggctgga gccgatccag aagggcgaca
     4201 tcgtcgtcaa cggcatttcg ctcaccgatc cgaagaccga cctgcccaag ctgcgcagca
     4261 aggtgggcat ggtgtttcag cacttcgagc tgtttccgca cctgtcggtg accgagaacc
     4321 tgacgattgc ccagatcaag gtgctcggcc gcagcgccga cgaggccaag acgcgcggcc
     4381 tgaagatgct cgaccgcgtg ggcctgatgg cgcacaagga caagttcccc ggccagctct
     4441 cgggaggcca gcagcagcgc gtggccattg cccgcgcgct gtcgatggac ccgatcgtga
     4501 tgctgttcga cgagccgacg tccgcgctcg accccgagat ggtgggcgag gtgctcgacg
     4561 tcatggtagg tctggccaag gaaggcatga ccatgatggt ggtcacgcac gaaatgggct
     4621 ttgcccgcaa ggtggccaac cgcgtgatct tcatcgacgt cggcggccgc attctggaag
     4681 actgctccaa ggacgagttc tttggccacc ccgagaaccg tcagccgcgc accaaggatt
     4741 tcctgaacaa gatcctgcag cattgacccc cctgaggcgc tggcgcgcct tcccccgtgg
     4801 ggacgcacgc agtggccggt gcaagcccgg ccacgcgcgc actggagcac tcctgcacct
     4861 gcggtgcagt cggttagatt gctcaatttt tgatagtggt ctgcgctgga ttggcgccgt
     4921 tttcaggaca aatgatctga aaccgtggca taccaagcgg gatcaggcag cgtccatcac
     4981 caactgcgtc tgcgtcacca ccgcgcacag cttgccttcg gcgttgcgaa cctcggtttg
     5041 ccagacgtgg gtgcggcgcc cgcggtgcag cgccacgctg tggccggtga ccgtgctgcc
     5101 gacgggcgcg ccgcgaatga acttggtgct ggagtgggtg gtggtcgtgc tctggccctt
     5161 ggcgaggctc atcacggtgc cgaccgcgcc cagcgtgtcg gcaaacgcca tgtaggcgcc
     5221 gccgtgcagg atgccgccag cggtgcaaag atcgggccgc accagcatct cggcgcgcac
     5281 ctcgtcgggc gatgcctgca gcacgcgcac gcccatcagg ccgggaaaca gcggcgcgag
     5341 caggtcgttc agttgatcgg gtgcgagcat gtgcgtctcc tcagcgtcat cgatcacaac
     5401 attatggagt tgaagaaaaa aacggcctca gcgcacgtcc atcaagcgca aacagctctt
     5461 ctatcgatag caaaaaagaa accccgccga agcggggttt gcagggctgg tcgacgcgct
     5521 tacttcttgc gcgccgctat cgccttctcg gccatgctca ccagatcggt gccgaccgtg
     5581 ctcttccact tgtcgtaggt tggtcgcgtg gccttgacga aggcgtcgcg ctcggcgggg
     5641 ctcaggtgcg tcaccgtcac gcccagggcg gcgatgtcct tcaagagcgg ctggcccggc
     5701 tcgatcatgc ctttgcgcgc aatgccgatc tgctccttgc cggcttcgat ggcggcctgg
     5761 cgcaccagtt cgcggtcggc cggcgtccac gattcccaca cctgcttgtt gaccacgaac
     5821 accagcgggt cggcaatgta gccccacatg gtcaggtact tctggcccac gttctgcagc
     5881 ttggcggcgg tgaagatgct catcgggttt tcctgcccat cgaccgcgcc gctggccagc
     5941 gcgggctgcg cgtcggccca gctcatctgc gtggggttgg cgcccagcgc ggtgaaggtt
     6001 tccaggaaca gcggcgagcc gacgacgcgg atcttcagcc ccttcaggtc ggccggcgtc
     6061 ttgacgacgt gcttggagtt ggtcagctcg cgatacccgt tttcgcccca ggccagcggc
     6121 accacgccgg ccttgtcgag gatgctgaac atctgcttgc cgacttcccc ctgcgtcagg
     6181 gcgtcgatgg cggcgtagtc gggcatcagg aagggcagcg agaacaggtt gagctgcttg
     6241 acctgcggcg accagttgat ggtggagccg acggccatgt cgatgacgcc ctggcgcagc
     6301 gcgctgaatt cgcgcgtctg gtcgccctgg atcagcgaca cgccggggta cagcttgatg
     6361 ttgatgcggc cattggtctt ttcgcgcacc ttgttggccc agatctcgcc gcccttgccc
     6421 cacggaaagg ccgggccgac aaccagcgac atgcggtatt cggacttgta gttctgcgcc
     6481 gcggcgggca gcgcaaaggt ggcgccagca agcgcggtgg tggcagcggc cagggccagc
     6541 agggtgcgac gaagtttcat gatcacggtc tccttgattg aatgaactgc aaaaacaata
     6601 gctggtggcg cttgctcctc aagcgccagc ggccaaaaac acttaaaaat caatacccca
     6661 gcacgcgcgg cagccacagc gccagttcgg gccagatgat gacggccacc atcaccaggc
     6721 ccatcgccag cagcatgggg ccgacccagc gcacggtttc ttccatgcgc acattcgcaa
     6781 tgcggcacga caccatcagg ttgaccgcca gcggcggcgt gaactggccc agcgcgactt
     6841 tcagcgtcag cagcacgccg aaccacaccg ggtcccagtt gtagtgctgc acgatgggcc
     6901 acaagagcgg cacgaagatg aggaagatcg acacgccatc gaggaacatg ccgatggtga
     6961 tcagcatcag gatgagcagc gccagcacgc cgtattcgcc cagccccgag ttgacgatgg
     7021 cgcgcgtgat cgggtcgatg acgcccagcg tcgacagcga aaacgcgaaa atgcccgcca
     7081 gcgagaccac cagcagaatc accgccgaca gctcgcccga ttccttgagg atggggaaca
     7141 ggtcgcgcaa cgtcatcgag cggtgcacga acaggccgac gaacagcccg tagaacacgg
     7201 ccaccaccgc cgcctcggtc ggcgtgaacc agcccatgcg catgccgccc agaatcagca
     7261 ctggcgcggc caggccccag atggcttcga tgaaactctt gagcagcggc gggcgcggca
     7321 gcgacgattc aaggtgcccc atgccgtgct tgcgcgccat ccacaccgcc ggcacgatga
     7381 gcgcgatgcc cgccagcacg cccggaatca tgccggcggc aaacagcgcc ggcaccgacg
     7441 cgcccggcac cagcaccgaa tagacgatga aggcgaccga gggcggaatc aggatgtctg
     7501 tggccgccgc cgcacccacc acgctggccg aatagctggc cgggtagccc gcgcggttca
     7561 tcgccgcgat catcaccgcg cccacggccg ccgcgttggc cgggccggag ccggagatgc
     7621 cgcccaggaa catcgccacg caaatcgcca ccagcggcaa catgcccggc ccgcgcccga
     7681 ccagcgcaat ggcaaaattg accagcctga gcgccacgcc cgagcggtcg aaaatcgagc
     7741 cgaccagcac gaacatcggg atcgccagca gcggatattt gcccaggccc gcgtaaaagt
     7801 tctgcggcac cgccagcagg ccgaaccacg gcgcctcggc gttcgccagc gcaatcgccg
     7861 ccgcgccggc cagcccgagc gacgcgccaa tcggcacgcc gagcatcatg gtgatgatga
     7921 agacggcaaa cagcagcgtc gcgatcatgc tgacatcgcc gtggtcgatt caaagaaaaa
     7981 ttggccactg gcgcttgctg gacaagcgcg agcagcttca ctttttatag tgatcatgaa
     8041 atccgacaga ctctcggcgc atgctcctca gcccgcactt cgggcgaggt cggcagctcg
     8101 gcgtcggccg ctgcgcgtcc gccgcgccgc atcaggccga tggcgcgccc catgatggcc
     8161 acgctcagga tcggcagcca catcgtgtac caccactgcg gcacgccgat gccgggcgag
     8221 gtttcgccgt actgcacgtc gtcccacagc acgcgcgccg acagcacggc gagcaggccg
     8281 aacagcagcg ccaccatggc ggcgccaaac ttggccagcg cgcgctgacg cgcttcgggg
     8341 ccggaatcgg caaagtattc gatgcggatg tgccggttgc gcgccaccgc cgccgagccg
     8401 gccaccatgg ccagcacgat catcaggaag accgacactt cttccgtcca cgcgaacgag
     8461 gcattggtga agtagcgcac cagcacgttg gcaaaggtga tcagcgccag cagcgccatg
     8521 atgatcaccg tgagccagtc ttcgatggcc agcggcacgc gcgtgggtgc gctgtcctct
     8581 ttcggcagag gcgcggccgc gttcggcgcg gattcgtggg gcaaggacat gaactgaaaa
     8641 aagcgcttgc ggtgcagcac gcaccgcccg cggcccaacg tggccgcaat ctggctatgg
     8701 taatcgcgcg ccggccgcct tttgggcgtt caaccggtat catgcgccca tgcaaaccct
     8761 tagcgacttc gcctgccgtg ccctggcgca cggcgcgttg gcccccgtgg ccgacgagcg
     8821 gtcgcacgcg cgtgtaccgc gcgcgcgggg gtttgcgcca tcgtcggcgc ggatcgcccc
     8881 gatccgtcgt gcgcgcctcg ccgccgcact ccgctgagcc cgacgacctc cgccgctctc
     8941 gcccagagca ccccaccggg tcgtcgggcc cacccgacca gcgccacggc gcgcctcacc
     9001 tttccttttc cgttgattcg ccctggcttg cgcgccggcg ggccaacccc agctgattga
     9061 ctgacgtttt caaggaatac cgccatgtcc accgccaccg acctgcaccg caccatcacc
     9121 gacctggacc acgtgcgcat cttcaacctg ctgcgccgcc agacggccgc cggcggcgcg
     9181 cccgagcacg cccgcgacct ggccgaactc atcgacgccg ccgagcaggt gcgcccgcag
     9241 gagatcgcgc ccgacgtggt caccatgctg tccaaactgc gcgtggccga tccggccacg
     9301 ggtgccgagc gcagcatcac cctgtgctac ccgcccgaag ccgatgcggc gcagggcatg
     9361 atttcggtgc tgtcgccgct cggcaccagc ctgctgggcc tgcgcgtggg tgaagtggcg
     9421 cgctggcgcg gcatcgacgg caaaacggct gaactcgtgg tgcgcgccat cgagttccaa
     9481 cccgaggcca gcggcgacta caccgcctga ggcgcctcgc accggacgat gaccggcatg
     9541 cgatggtggc taagcaaacg ctcacatcct ggcgctcagc cggcgcttgc gctacgcggc
     9601 ttgcaggcgc cgtccttcaa gagggcagga aaggcacccg catggcgggc ctggcaaccg
     9661 cttttcaata gggcctcagc ggcgccttgc cgcgctgaaa cactcgaagg agcaaacaca
     9721 tgttgaagag ccaacccccg gtttccgtca aaggcgtggc cgccatnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnntggg tgccgggcta ctgggcgttc agcaccacca
     9901 acaacgcgtg ggtgtggacg ccgggcgctt ggaggaacta agcttcccct gagtcggcct
     9961 gcggccgcca tcccccgaag gggacgatgc cagtggccgg gccaagcccg ttccacggca
    10021 tcccaggatg gccatctccg cggccctctg atattgagaa aaagccatcg tcctgcggcg
    10081 atggcttttt tgttggcgcg gtgcgatggg acaatctgcg catgagcact gccccgcaaa
    10141 ccctcaccat cacccggccc gacgactggc atttgcacgt gcgcgacggc gcggcgctgg
    10201 ccgatgtggt gccgcacacg gcggcgcagt tcgggcgcgc catcatcatg cccaacctgc
    10261 ggccgccggt caccaccacg gcgcaagcgc tatcgtataa agagcgcatt gcgcaagcgg
    10321 tgcctgcgca agcggccttt gagccgctga tgacgctgta tctgaccgac aacacgccgc
    10381 ccgatgagat cgcccgcgcg gccgatgcgg gcatcgtggc gctcaagctc tatcccgctg
    10441 gcgccaccac caactcggac gccggcgtca ccgacatccg caagacctac aaagtgctcg
    10501 aagccatgcg gcgcgccggc ctcaagctgc tggtgcacgg tgaggtgacg gatcccgaca
    10561 tcgacctgtt cgatcgcgaa gccgtgttca tcgaccgcgt gatgattccg ctgcggcgcg
    10621 acttccccga gctgaaggtg gtgttcgagc acatcaccac gcgcgacgcc gcgcactacg
    10681 tcgccagcgc cgacgcccac accgctgcca ccatcacggc gcaccacctg ctctacaacc
    10741 gcaacgccat cttcgtcggc ggcatccgcc cgcactacta ctgcctgccg gtgctcaagc
    10801 gcgaagtgca ccgccaggcg ctggtgcagg cggcgactgg cggatcgtcc aaattcttcc
    10861 tcggcaccga cagcgcgccg cacccggcgc agctgaagga gcacgccagc ggctgcgccg
    10921 gctgctacac cgcgcacgcc gcgctggagt tgtacgctgc cgcgttcgaa gcggctggcg
    10981 cactggacaa gctggagggc tttgcgtcct tccacggccc cgacttctac ggcttgccgc
    11041 gcaacggcgg caccgtgacg ttgaagcgcg aaagctggac gccgcccgac agctacccat
    11101 tcggcggcgc cgaggtcaag ccgctggccg gcggcgaagc gctcgcttgg cgcctggcgg
    11161 catgagagaa gccatgacca aaccctctct tgctgacttc gcagtcacca cgctcgatgg
    11221 ccagccgctg aacttggccg atctgactgg acgcgtgctg ctcatcgtca acaccgccag
    11281 cgaatgcggg ttcacaccgc agtacgccgg cctgcaggcg ctgcacgagc gttacggcgc
    11341 ccaaggtctg acggtgctgg gcttcccctg caaccagttc ggcgcgcagg agccgggcga
    11401 tgcagggcag atcggcgcct tttgccagcg caactacggc gtcggctttc cgatgatggc
    11461 caaggtcgat gtcaacggcg cccacgccgc gccgctgttc cgctggctga aagagcaggc
    11521 acccggcctg ctgggcagcc aggcgatcaa gtggaacttc accaagttcc tgatcgggcg
    11581 cgacggccgc gtgattcgcc gctacgcgcc gcaagacaag cccgaaagcc tggcgcgcga
    11641 catcgaggtg gccttggcgg cgtagaccgc taccgcaccg caaacccctc ggcgcagtgc
    11701 ttgcgtccat gctcgttgta gacgatggca atggccggcc cgaccgacag cagcagcaag
    11761 ccccagggca gcgccgcgcc cagccacacc gccagcgggc ccagcgccac gcaggccagg
    11821 cacagcagcg ccagcccgcc ctgactggct tcggcgcgcg tggtgcgggt gtagatgacc
    11881 cacagcgaga acatgaacag cgcctgcgcc agcgccacga tgctgatggc gtaaagcggc
    11941 gacacgccat gtgccgcatt gcccgcgtgg cccgctggcg caagcgcgtg gcccccgtcg
    12001 gcgatgccgg cggcggcttg cttgccctcc tccagcacat cggccaccac ctccagcccg
    12061 gcgcccacgg ccgccgccga ggcaaagatg aagaagtgcc cgtagcccca aaaaaagccg
    12121 cgctcgcggt gccggtgcag cgcatcgccc gagggcagca agaaatacat ccaccacagc
    12181 gcaaacgcca gcatggtgct gcccaggccg accagcgcca ggttcagcgt ccagccaaag
    12241 ctttgccact tgcctgccac cgcgttggtg gcgcccagca ccacctcgcc cagcgtgatg
    12301 atgagcagca ggctgtagcg ctcggcaatg tggtgggcgt gccagggcgt gcggtgccgg
    12361 cgttcggcga tgatcggcac cgccagctcg cacgccatga acagcacgaa ggtgggccac
    12421 aaccattcca ccgggaacca cagccgcgcg atccacagcg cctgcaccag cgtgatgccg
    12481 agcgcgaagc ggcggcaggt ggcggcgcac gccgggtcgc cgcgcgcggc cagcagccac
    12541 tgcaccacca gcgccagccg catcaccgca tagtccagca cgccagcgcg aaagtcgccg
    12601 ttgaacagcc ccggcacgcc ggccgcgaag atcagcacgc ccaccgtctg cagcatggtc
    12661 agcagacgga acgccgcgct gtcgtcggcg taggccgagg caaaccacgt gtagttcatc
    12721 cacgcccacc agatggccga gaacgccgcc acaaagccgg ccagcgccgg gccggtgtgg
    12781 tgctccatca cgccgtggtg cagctgcgag gcggccaacg cgatggcgac cacgaagctc
    12841 aggtcgaaca gcagctccag cggggtcgcc acgcggtgct gctcgtggat gtcgcgtccg
    12901 cgcgcccgag ccgtgggcgc cgcgggaaga ggggtggggg cgttcttcgg tggcgtgtgg
    12961 caggatcggc cggcgatggt aactgaccga ccctcagaag cacccgccgc cccgccgatc
    13021 gactggcacg cgccctggtg ggcgccgttg gcgccgcgcg gcgcgccggc gctggcgcgc
    13081 tgggccggcg
//
LOCUS       DAOMVO010000033        13034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1086_length_13034_cov_15.745050, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000033 DAOMVO010000000
VERSION     DAOMVO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1086_length_13034_cov_15.745050"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1477
                     /locus_tag="PKV30_03115"
     CDS             146..1477
                     /locus_tag="PKV30_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRL29243.1"
                     /translation="MTSLTLFLPCAGGVEDFLADEVHGLTGLAGDDLLTVRGGVRVRA
                     GWAEAMRLNLHSRLAQRVLIELAHGAYRDEHDLYALAAGVAWEDWFTPRQTFRVDVTA
                     QSSPLKSLNFATLRIKDAVADRFRERAGGVRPSIDTHAPGVRVQAHLGPAEATLYIDT
                     SGEPLFKRGWRQDKGDAPLKETLAAAMLAASGWWQPAERRVSDQPLYDPCCGSGTIVI
                     EAAQLRLGLPAGGLRRFAFERLKPFDAVQWNTIKKEAARADQTSAGGQNDSRTPVQVF
                     GSDVAHRMVDFAQRNAERAGVAHAVQLRGGDALQRLPPTGLPGVLLMNPPYGERIAAA
                     GVAGVRAAGGREAFQSSAPAADEFNAGDEFFARLAAHWKGHYAGWTAWLLTPDLKLPG
                     RLRLKESRRVPLWNGPIECRLFRFDLTARQSVDSASKAPPALDGQAPPAPD"
     gene            1495..1941
                     /locus_tag="PKV30_03120"
     CDS             1495..1941
                     /locus_tag="PKV30_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL29244.1"
                     /translation="MNNPEAAPGAVVIDTNIALDLLVFADPAVQALHTALRAGTLRWL
                     ATARMRAELARVLGYPRIAPRLAVAGLPAVAVLTAFDALTQGMPEPPSCASARCADPD
                     DQPFIDLAVTHGALLLSKDAAVLGLTRRLRAQGIHVMSKWPPALAE"
     gene            1985..3556
                     /locus_tag="PKV30_03125"
     CDS             1985..3556
                     /locus_tag="PKV30_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL29245.1"
                     /translation="MSDAFKLTYSTMFNPPPELHARFDAALAEFKHSGMGRDHPQWID
                     GAAAEGGERFEVRSPIDQDWLIGRFVQGSADDVDRAVQAARAAYPAWAATPWRERVAL
                     LRRAARLIEERVYAISAAVAVEVGKNRMEAIGEVQETADLIDWYADRMEEADGFDRPL
                     ADDPLPNFKSHNRSVLRPYGVWAVIAPFNFPFALAGGPLGPALIAGNTAVVKCSPETS
                     LSGWLLLECLRDAGLPPGVINFVTGGDTTGRALAQHPDVAGITFTGSHAVGMQVLRAS
                     AAGRYPKPCITEMGGKNATIVSRHGDVERAALGILRSAFGLSGQKCSACSRVYVERPL
                     ADALRERLVQLTRDIAVGDPTQQAHWMGPVIDRGAYDRYARLAKHLRQVGQIDTGGAW
                     LTEGDLARGFFVAPTVAQAPLDDRAWVDEHFLPVVLVGEVDSVAEGIARANASDYGLT
                     AGFYGTPEEVAYFHNHIEAGVTYANRPQGATTGAWPGFQPFGGWKGSGSTGKAIGSFH
                     YLQQYLREQSRTVVE"
     gene            complement(3676..4569)
                     /locus_tag="PKV30_03130"
     CDS             complement(3676..4569)
                     /locus_tag="PKV30_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL29246.1"
                     /translation="MAAVFIGAPLVGVYPVFMMKVMCFALFACAFNLLLGFGGLLSFG
                     HAMFLGGAGYAAAHAAKVWGFTPELAVLFATLCAAALGWLTGKLAIRRQGIYFAMITL
                     ALAQMVFFLALQAPFTGGEDGIQAVPRGKLFGLIDLSNVNVMYAFVLAIFLGGFLLIY
                     RIVHSPFGQVLKAIRENEPRAISLGYDADKFKHRAFVLSAALAGLAGATKAIVFQLAS
                     LTDVHWSMSGEVVLMTLVGGLGTIFGPVVGAAVIVTMQNYLAQLGAWVTVVQGVIFVV
                     CVLLFRRGIVGEIGHLLKKSL"
     gene            complement(4663..5547)
                     /locus_tag="PKV30_03135"
     CDS             complement(4663..5547)
                     /locus_tag="PKV30_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL29247.1"
                     /translation="MEIFGIPIQAMMGQLLIGLINGSFYALLSLGLAVIFGLLNIINF
                     AHGAQYMLGAFGAYLLLNKLGLGYWWALLIVPLVVGATGVIIERTMLARLYKLDHLYG
                     LLLTFGLALIIQGFFRNEYGSTGLPYAMPEQLQGGINLGFMFLPIYRGWVIIASLIIC
                     LATWFVIERTKLGGYLRAATENPQMVQAFGINVPRMITLTYGFGVALAALAGVMAAPI
                     YQVSPQMGADLIIVVFAVVVIGGMGSIMGAIVTGFGLGLIEGLTKVFYPEASTTVIFI
                     IMTIVLLIRPAGLFGTQK"
     gene            complement(5592..6812)
                     /locus_tag="PKV30_03140"
     CDS             complement(5592..6812)
                     /locus_tag="PKV30_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL29248.1"
                     /translation="MKRKLLSTLIAGLGLAGAGMAQAQISGNVVKIGVLSDMSGLYAD
                     IGGPGSVVAAKMAVDDYLKASKSQLKVEVVSADHQNKPDVGSSVARKWYDTDGVDMII
                     DVPTSSVGLAINQVTKEKGKAYINTGSATSDLTGKDCSPNTVHWLYDTWMLANGTGSA
                     VVKSGGDTWFFLTADYAFGHALERDTSEVVKASGGKVLGSVRVPLATQDFSSFLLQAQ
                     SSKAKIVGLANAGGDTINSIKQASEFGITKGGQKLAGLLVFLPDVHGLGLNVAQGLQI
                     TEAFYWDLNDQTRAWSKRFAAANGGKYPSSDHAGVYAGALHYLKAVDAAKTDDGAKIV
                     AKMKELPTDDPLFGKGQVRADGRKIHPVHLFEVKKPSESKGPYDYYKVLQTIPADKAF
                     RPIDQGNCPLVAKK"
     gene            complement(6875..7588)
                     /locus_tag="PKV30_03145"
     CDS             complement(6875..7588)
                     /locus_tag="PKV30_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29249.1"
                     /translation="MSTSNTPAIRLKDVHSWYGESHIMHGVSIDVMPGEVVTLLGRNG
                     AGRTTTLRAIMGLVGKRTGSIQINGVETVRMAPHQIARLGLGYCPEERAIMTSLSCQE
                     NLMLPPKVADGGMSVDEIYEMFPNLLSRKNSPGGRLSGGEQQMLAVARILRTGAKTLL
                     LDEISEGLAPVIVQALARMIRELRARGFTVLMVEQNFRFAAPLADRFYIMERGLIVRE
                     FSAAELQGNMEMLREYLGV"
     gene            complement(7599..8372)
                     /locus_tag="PKV30_03150"
     CDS             complement(7599..8372)
                     /locus_tag="PKV30_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29250.1"
                     /translation="MSSDIILETRGLVKEFKGFVAVNDVNLKVRRGTIHALIGPNGAG
                     KTTCFNLLTKFLEPTRGQILFDGHDITRDKPADIARRGVVRSFQISSTFPNMSVLENV
                     RVALQQTLGTAFSFWKSERTLNQLNERCMELLGFVGLADYAHLKAMELPYGRKRALEI
                     ATTLAMDPKLMLLDEPTQGMGSEDVDLVTQLIKKVAANRTVLMVEHNMGVVGNIADQI
                     TVLQFGQVIAEGPYAEVSRNPQVLEAYMGSADEELVGAH"
     gene            complement(8603..8836)
                     /locus_tag="PKV30_03155"
     CDS             complement(8603..8836)
                     /locus_tag="PKV30_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29251.1"
                     /translation="MRHASATRKSNAPASAQEVARQLRPRQAPEPGPESRVVPEQAPA
                     PNVAPASPTEVPPTWHGSDANERAQPAPPPVAP"
     gene            complement(9381..9920)
                     /locus_tag="PKV30_03160"
     CDS             complement(9381..9920)
                     /locus_tag="PKV30_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRL29252.1"
                     /translation="MVNRMLQKKELLALDGQAPQAEIIQNIADALRQRGWLLATAESC
                     TGGLIAAACTDLAGSSDWFERGFVTYSNAAKTELLGVPPALIAQHGAVSEPVVRAMAA
                     GAVAHSAAHVSVAVTGVAGPTGGSEDKPVGTVWFGWCVGGQCSTERQLFAGDRASVRT
                     QSAQHALAGLLARVRTVAD"
     gene            complement(9914..10387)
                     /locus_tag="PKV30_03165"
     CDS             complement(9914..10387)
                     /locus_tag="PKV30_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HRL29253.1"
                     /translation="MRGHPAHWIALAFGAGLSPVAAGTVGTLWAWLVWALLLAPLAPA
                     VQGAVIALTLLAGWWAATVTARHLQSADPGAIVVDEVIAFWIVLWLVTPAGFWTQLVA
                     FALFRFFDAVKPGPVGWADRLLHGAHGWRGGFGILLDDLVAAFCTLLVMALWRAW"
     gene            complement(10555..12195)
                     /locus_tag="PKV30_03170"
     CDS             complement(10555..12195)
                     /locus_tag="PKV30_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRL29254.1"
                     /translation="MHLKTLAHSGPHWRLLLPMLALCALSPAQAQSTETRADASRVIR
                     VKVYPGSATVERVLRVAPGARQATFACLPAGLDAASLQVASDTNVRVGELAVRQQARE
                     LLGKACANPLEDRIGALEDQIAALTAESAGIGYAAGYFKSFENAASGADSRTAPAQIG
                     ATAQALRQSARDALTRQHQIKRQQEALEAELKPLVAERDRAGTQKALVSTVQVTLAAP
                     QGGELRLSYQVRGPGWQPSYRATLDSASKKVRLERQALVAQNTGEDWDGVQLTLSTGQ
                     PGAATQGPLPRPWRIGIEPPPPPQAPVAAMAGAPMPAPAPVARMRATTAESAEAMPSF
                     DVSVFQGSFATEFAVPQKISVPSSGQRITLSLGEQTLDARLLVRATPALDASAYLIAA
                     IAPPPGVWPAGPISLYRDGAYVGNGRFDTAQLARNGLAFGRDELVTVRMEQPAQKEGT
                     GGFIGSRNERRVNRLYTAENRHREAVTLQLLDAAPVAEHEDVRVESRYQPEPQTKAWN
                     EQPGSVMWEQPLAAGAMQRFSAEHVITWPKDARLRERQ"
     gene            complement(12280..12558)
                     /locus_tag="PKV30_03175"
     CDS             complement(12280..12558)
                     /locus_tag="PKV30_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRL29255.1"
                     /translation="MPFAPSERQALLAVKGVGPTVIARLEQMGIESLAHLARANSADV
                     LARGAALAGSSCWNNSPQARTAIHGAIAAAQEATRQPRSSGTQSLPNS"
     gene            complement(12558..>13034)
                     /locus_tag="PKV30_03180"
     CDS             complement(12558..>13034)
                     /locus_tag="PKV30_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HRL29256.1"
                     /translation="MGKVPAIQHGDTVVTENAAILLYLADLVPEKKLAPPVGTPARGP
                     YYRWIAFMAPLEAMLTAKSAGALAKPEQAGYGSEADVLATLEGALKGRDHLAGDHFTA
                     ADLFVAASIGFYLQFKMLEPRPDFVRFAELHAQRPAALRANAIDDEAAAKLQAATG"
BASE COUNT         2107 a         4322 c         4495 g         2110 t
ORIGIN      
        1 tggcctacac ctacgaagac ccgttcgtga ccatgacggc gcggctgccg ctggtgttgc
       61 cctgatgacc gcgctcgtca gagcgcagtc atcgtttgac acaatcggcc cactggctcc
      121 actgcggcgc cgctgattca accccatgac atccctgacc ctgtttctgc cctgcgccgg
      181 tggcgtggaa gactttctgg ccgacgaagt gcacggcctg acgggcctcg cgggcgacga
      241 tttgctcacc gtgcgcggcg gcgtgcgtgt gcgcgccggc tgggccgagg cgatgcggct
      301 gaacctgcac agccggctgg cgcagcgcgt gctgatcgag ctggcgcacg gcgcctaccg
      361 tgacgagcac gacctgtacg cgctggccgc cggcgtggcg tgggaagact ggttcacgcc
      421 gcgccagacc ttccgcgtcg atgtcaccgc gcagtccagc ccgctcaaaa gcctgaactt
      481 cgccaccctg cgcatcaagg acgcggtggc cgaccgcttt cgcgaacgcg ccggcggcgt
      541 gcgccccagc atcgacaccc acgcgcctgg cgtgcgcgtg caggcgcacc tgggcccggc
      601 cgaggccacg ctgtacatcg acaccagcgg cgagccgctg ttcaagcgcg gctggcggca
      661 ggacaagggc gacgcgccgc tgaaggaaac gctggccgcc gccatgctgg ccgccagcgg
      721 ctggtggcag ccggccgagc ggcgcgtcag cgatcagccg ctgtacgacc cctgctgcgg
      781 cagcggcacc atcgtcatcg aggcggcgca gttgcggctg ggactgccgg ccggcgggct
      841 gcgccgcttt gccttcgagc ggctcaagcc cttcgatgcc gtgcagtgga atactataaa
      901 aaaagaagct gctcgcgctg atcagacaag cgctggcggc caaaatgatt caagaacgcc
      961 ggtgcaggtg ttcggcagcg acgtcgcgca ccgcatggtc gatttcgcgc agcgcaacgc
     1021 cgaacgggcc ggcgtggccc acgccgtgca gttgcgcggc ggcgacgcgc tgcagcgcct
     1081 gccgcccacc ggcttgccag gtgtgttgct gatgaacccg ccttatggcg agcgcattgc
     1141 cgccgctggc gtggccggcg tgcgtgccgc cggcgggcgc gaggccttcc agagcagcgc
     1201 gccggcggca gacgagttca acgccggcga cgaattcttc gcccgcctgg ccgcgcactg
     1261 gaagggccat tacgccggtt ggacggcgtg gctgctcacg cccgatttga agctgcccgg
     1321 ccgcctgcgc ctgaaagaat cgcgccgcgt gccgttgtgg aacgggccga tcgagtgccg
     1381 gttgttccgg ttcgatctga cggcgcggca gagcgtcgat tcagcgtcaa aagcaccgcc
     1441 agcgctggat ggacaagcgc caccagctcc cgattgaata gcgattcaca gcgagtgaac
     1501 aacccggaag cggcgccggg cgccgtcgtc atcgacacca acatcgcgct ggacctactg
     1561 gtgtttgccg accccgccgt gcaggcgctg cacaccgcct tgcgtgccgg cacgctgcgc
     1621 tggctggcga ctgcgcgcat gcgggccgag ctggcgcgcg tgctgggcta cccgcgcatc
     1681 gcgccgcgcc tggcggtcgc tgggttacct gccgtcgcgg tgctgaccgc ctttgacgcg
     1741 ttgacgcagg gcatgcccga gccgcccagt tgcgcatcgg cccgctgcgc cgaccccgac
     1801 gaccagccct tcatcgacct ggccgtgacg cacggcgcgc tgctgctcag caaggatgcc
     1861 gcggtgctgg gcctgacgcg ccgcctgcgt gcgcaaggta tccatgtgat gtcaaaatgg
     1921 ccgccagcgc ttgctgaata agcgcaagca gctatcaaat taatactaaa aggagaccca
     1981 ccgcatgagc gatgcgttca agctgaccta ttcgaccatg ttcaacccgc cgcccgagtt
     2041 gcacgcgcgc ttcgacgccg cgctggccga gttcaagcac agtggcatgg ggcgcgatca
     2101 cccgcagtgg atcgacggcg ctgcggcgga gggcggcgaa cgcttcgagg tgcgctcgcc
     2161 catcgatcag gattggctca tcggccgctt cgtgcaaggc agcgccgacg atgtggaccg
     2221 cgccgtgcag gccgcgcgcg ccgcttatcc ggcgtgggcc gccacgccgt ggcgcgagcg
     2281 cgtggcgctg ctgcggcgcg ccgcgcggct gatcgaagag cgcgtgtacg ccatcagcgc
     2341 cgccgtggcg gtggaagtgg gcaagaaccg catggaggcg attggcgagg tgcaggagac
     2401 ggccgacctg atcgactggt acgccgaccg catggaagaa gccgacggct tcgaccgccc
     2461 gctggcggac gacccgttgc caaacttcaa gagccacaac cgcagcgtgc tgcggccgta
     2521 cggcgtgtgg gcggtgatcg cgccgttcaa ctttcccttc gcgctggccg gcgggccgct
     2581 cgggccggcg ctgatcgcgg gtaacacggc ggtggtcaag tgctcgccgg agacgtcgct
     2641 ctcgggctgg ctgctgctgg aatgcctgcg ggatgcgggc ttgccgccgg gcgtgatcaa
     2701 cttcgtgacg ggcggcgaca caacgggccg cgcgctggca cagcaccccg atgtggcggg
     2761 catcaccttc accggctcgc acgcggtggg catgcaggtg ctgcgcgcca gtgccgcagg
     2821 ccgctacccc aagccctgca tcaccgagat gggcggcaag aacgccacca tcgtcagccg
     2881 acatggcgac gtggagcgcg cggcgctggg cattttgcgc tcggcattcg ggctgtcggg
     2941 gcagaagtgc tcggcctgct cgcgcgtgta tgtggagcgc ccgctggccg atgcgctgcg
     3001 cgagcggctg gtgcaactga cgcgcgacat cgcggtgggc gacccgacgc agcaggccca
     3061 ctggatgggg ccggtcatcg accgcggcgc ctacgaccgc tatgcgcggc tggccaagca
     3121 tttgcggcag gtcgggcaga tcgacaccgg cggcgcgtgg ctgaccgaag gcgatctcgc
     3181 gcgcggcttt ttcgtcgcgc ccaccgtggc gcaggcgccg ctggacgacc gcgcgtgggt
     3241 cgacgaacac ttcctgcccg tggtgctggt gggcgaggtc gattcagtgg ctgaaggcat
     3301 cgcacgcgcc aacgccagcg actacgggct gacggcgggc ttttacggca cgccggagga
     3361 agtggcgtac ttccacaatc acatcgaggc cggtgtgacc tacgccaacc gcccgcaagg
     3421 cgccaccacg ggcgcgtggc cgggttttca accctttggc ggctggaagg gcagcggcag
     3481 cacgggcaag gcgatcggct cctttcacta ccttcagcag tacctgcgcg agcagtcgcg
     3541 cacggtggtg gaatgaatgg tgctacaact gtcgtagctg ctcgcgcttg ttggacgagc
     3601 gccagcgcct gatttgattc aagaaaacaa aacgcgggcc gtggcccgcg ttgtctggtc
     3661 ggggaatgcg cccactcaga gcgacttctt cagcaggtgc ccgatctcgc ccacgatgcc
     3721 gcggcggaac agaagcacgc agacgacgaa gatcacgccc tgcaccaccg tcacccaggc
     3781 gcccagttgt gccagatagt tctgcatggt gacaatcacc gccgccccaa cgaccgggcc
     3841 gaagatggtg cccagcccgc ccacgagcgt catcagcacc acctcgcccg acatgctcca
     3901 gtgcacgtcg gtcagtgaag ccagttggaa cacgatggcc ttggtggcgc ccgccaggcc
     3961 cgccagcgcg gccgacagca cgaaggcgcg gtgcttgaac ttgtcggcgt cgtagccgag
     4021 cgaaatggcg cgcggctcgt tctcgcggat cgccttcagc acctggccga agggcgagtg
     4081 cacgatgcgg tagatcagca ggaagccgcc caggaagatg gccagcacga aggcgtacat
     4141 gacgttcacg ttcgacaggt cgatcagccc gaacagcttg ccgcgcggca ccgcctgaat
     4201 gccatcctcg ccgccggtga agggcgcctg cagggcgagg aagaacacca tctgcgccag
     4261 cgccagcgtg atcatggcga agtagatgcc ctggcggcga atggccagct tgccggtgag
     4321 ccagcccagc gccgcggcgc acagcgtggc gaacagcacg gccagctcgg gcgtgaagcc
     4381 ccacaccttg gcggcatgcg ccgccgcata gccggcgccg cccaggaaca tggcgtggcc
     4441 gaacgacagc aggccgccaa agcccagcag caggttgaag gcgcacgcaa acagcgcgaa
     4501 gcacatcacc ttcatcatga acacggggta cacgccgacc agcggcgcgc cgatgaacac
     4561 cgccgccatg atgatgaagg cgacgcggtg cgtgcgctcg tcgctggcgg tgtcggccgg
     4621 cttgggggtg gaggtaatgg gggtcgatgc ggtggccatg gcttacttct gtgtgccaaa
     4681 caagccggcc ggacggatca gcagcacgat ggtcatgatg atgaagatga cggtcgtcga
     4741 ggcctcgggg tagaacacct tggtcaggcc ctcgatcaag cccagcccga agccggtgac
     4801 gatggcgccc atgatggagc ccatgccgcc gatcacgacc acggcgaaca cgacgatgat
     4861 gagatcggcc cccatctgcg ggctgacctg gtaaatgggt gcggccatga cgccggccag
     4921 cgccgccagc gcgacgccaa agccgtaggt cagcgtgatc atgcgcggca cgttgatgcc
     4981 gaaagcctgc accatctgcg ggttctcggt ggccgcgcgc agatagccgc ccaatttggt
     5041 gcgctcgatg acgaaccaag tcgccaggca gatgatgagc gaggcgatga tgacccagcc
     5101 gcggtagatc ggcaggaaca tgaagcccag gttgatgccg ccttgcaact gctccggcat
     5161 ggcgtagggc aggccggtgg agccgtattc gttgcggaag aagccctgga tgatgagggc
     5221 caggccgaag gtcagcagca gaccatacag gtgatccagc ttgtacaggc gcgccagcat
     5281 ggtgcgctcg atgatcacgc cggtggcacc gaccaccagc ggcacgatga gcagcgccca
     5341 ccagtagccc aggcccagct tgttcagcag caggtaggcg ccaaacgcgc ccagcatgta
     5401 ctgggcgcca tgggcgaagt tgatgatgtt gagcaggccg aagatgacgg ccagccccag
     5461 cgacagcagg gcgtagaacg agccattgat caggccgatg agcaactggc ccatcatggc
     5521 ctggatcggg atgccaaaga tttccatgtt tcgttcgtca gccgtgcggt cgcgtgtctt
     5581 tcaagccgag cttacttctt ggccaccagc gggcagttgc cctggtcgat cgggcggaac
     5641 gccttgtcgg ccgggatggt ctgcaacacc ttgtagtagt cgtaggggcc cttggattcg
     5701 ctcggcttct tgacctcgaa caggtgcact gggtggatct tgcgaccgtc ggcgcgcacc
     5761 tggcccttgc cgaagagggg atcgtcggtc ggcagttcct tcatcttggc gacgattttg
     5821 gcgccgtcgt ccgtcttggc ggcatcgacc gccttcaggt agtgcagcgc gcctgcgtag
     5881 acaccagcgt ggtccgacga agggtacttg ccgccgttgg ccgccgcgaa gcgcttggac
     5941 caggcacggg tctgatcgtt caggtcccag tagaaggctt cggtgatctg cagaccctgc
     6001 gccacgttca ggcccagccc atgcacgtcg ggcaggaaca ccagcaggcc ggccagcttc
     6061 tggccgccct tggtgatgcc aaactcggat gcctgcttga tcgagttgat ggtgtcgccg
     6121 ccggcgttgg cgaggccgac gatcttggcc ttggacgact gcgcctgcag caggaaggac
     6181 gagaagtcct gcgtcgccag cggcacgcgg acgctgccca gcaccttgcc gcccgaagcc
     6241 ttgaccactt ctgacgtgtc gcgctccagc gcgtggccga aggcgtagtc ggcggtcagg
     6301 aagaaccaag tgtcaccacc gctcttgacc acggcgctgc cggtgccgtt ggccagcatc
     6361 caggtgtcgt acagccagtg cacggtattg ggcgagcagt ccttgccggt caggtcggac
     6421 gtggcagagc cggtgttgat gtaggccttg cccttttcct tggtgacctg gttgatcgcc
     6481 aggccgaccg aggaggtggg cacgtcgatg atcatgtcga cgccgtcggt gtcgtaccac
     6541 ttgcgggcca cgctggagcc gacgtcaggc ttgttctggt gatcggccga aacgacctcg
     6601 accttgagct gggacttgct ggccttcagg taatcgtcca ccgccatctt tgcggccacc
     6661 accgagcccg gcccgccgat gtcggcgtac aggcccgaca tgtcgctgag cacaccgatt
     6721 ttcaccacat tgcccgaaat ctgggcctgc gccatgccgg cgcctgccag gcccaggccg
     6781 gcgatcagcg tgctgagcag tttgcgtttc atcattactt ctcctttgaa gatggactgg
     6841 caagaaagac tacggacagg gaaaccaaaa acagtcaaac gcccaggtac tcgcgcaaca
     6901 tttccatgtt gccctgcagc tcggcggcgg aaaactcgcg cacgatcagg ccgcgctcca
     6961 tgatgtagaa gcggtcggcc agcggcgccg caaagcggaa gttctgctcg accatcagca
     7021 ccgtgaagcc gcgtgcgcgc aactcgcgga tcatgcgcgc cagcgcctgc acgatgaccg
     7081 gcgccaggcc ttcggagatt tcgtccagca gcaacgtctt ggcgccggtg cgcaggatgc
     7141 gcgccaccgc cagcatctgc tgctcgccgc ccgagaggcg cccgccgggg ctgttcttgc
     7201 gcgacagcag gttggggaac atctcgtaga tctcgtccac gctcatgccg ccgtcggcca
     7261 ccttgggcgg cagcatcagg ttttcctggc agctgagcga ggtcatgatg gcgcgctctt
     7321 ccgggcagta gcccagcccc aggcgcgcga tctggtgcgg cgccatgcgc acggtttcaa
     7381 cgccattgat ctggatcgag ccggtgcgct tgccgaccag gcccatgatg gcgcgcagcg
     7441 tggtggtgcg gccggcgcca ttgcggccca gcagggtgac gacttcgccc ggcatgacgt
     7501 cgatgctgac gccgtgcatg atgtgcgact cgccgtacca cgagtgcacg tccttcaggc
     7561 ggatggcggg ggtgttggat gtgctcatgc gctgcaactc agtgggcacc gaccagctct
     7621 tcgtcggcgc tgcccatgta ggcttccaac acctgcgggt tgcgcgagac ttcggcgtac
     7681 gggccttcgg caatcacctg gccgaactgc agcaccgtga tctggtcggc aatgttgccg
     7741 accacgccca tgttgtgctc gaccatcagc acggtgcggt tggccgctac cttcttgatc
     7801 agttgcgtga ccaggtctac gtcttccgag cccatgcctt gcgtcggctc gtccagcagc
     7861 atcagcttgg ggtccatcgc cagcgtggtg gcgatctcga gcgcgcgctt gcggccgtag
     7921 ggcagctcca tcgccttcag gtgcgcgtag tcggccaggc cgacaaagcc gagcagctcc
     7981 atgcagcgct cgttcagctg gttcagcgtg cgctcggact tccagaagct gaaggcggtg
     8041 cccagcgttt gctgcagcgc cacgcgcacg ttctccagca cgctcatgtt ggggaaggtg
     8101 gacgaaatct ggaacgagcg caccacgcca cggcgcgcaa tgtcggccgg cttgtcgcgc
     8161 gtgatgtcgt ggccgtcgaa caggatttgc ccgcgcgtcg gctcgagaaa cttggtcagc
     8221 aggttgaagc aggtggtctt gccggcgccg ttggggccga tcagcgcgtg gatggtgccg
     8281 cgacgcacct tgaggttgac gtcgttgacg gccacgaagc ctttgaactc tttgacgagt
     8341 ccccgcgttt ccaaaatgat gtccgaactc atgcgggccc gtcgcgcctt gaagacagtg
     8401 aaaaacgggc ggattatgcg tgacgcatcg cagcaagccg ataggggttt ttacccggcg
     8461 aaaccatgcg cggcgtcaat gaacacgcac catttccggg cttgcgggtc acatcgtccc
     8521 cgtggtgaag cttattaaca ctttaggcct ctggtgcttg aagggcaagc gcgagcagct
     8581 atcaaatcaa tagttttgac cgtcagggcg caaccggcgg cggcgccggt tgggcgcgct
     8641 cattggcatc cgaaccgtgc caggtcggcg gcacttcggt gggcgaggcc ggcgcgacgt
     8701 tgggcgccgg cgcctgctcg ggcacgacgc gcgattcggg ccccggttcg ggcgcctggc
     8761 gcgggcgcaa ctggcgcgcc acttcctgtg ccgacgctgg cgcattgctc ttgcgcgtgg
     8821 ccgaggcgtg gcgcaaatcg ggcggcgcct catcgggcgc cggctcgcgc ggtgcaaggc
     8881 tgtgcgcgtg aagtattacg cctgtgatct caacgacgcg cagacggtcg tgtcgaactg
     8941 cgctgcgacc gacacgggca cctacgcgat cagcacctca atggcgctcg cgtgatgcag
     9001 ttcgccgggc atgttgtcac ctcgatgaat cacacccgag gctacgccga ggtcgactgg
     9061 ggcggcgcga atggcaagtg gatctatgtc tatcggcagc tcaaaccggc gcagaactac
     9121 cgactgggct cctccaaccg cctgaacgcc gctgcctggg cggccatgaa gacgcaggtt
     9181 acggggccgc aggcaacgac atcacggtct acttctctgg ccgttccgac aagggcactt
     9241 ggcgcgcgga ggatggtcgc atctgctacg aattcacgac catcccctct gcctgcaacg
     9301 acgtgcggct gctcggcaac gacatctacc tgaagcgcag caacgggcag gtgacgcagc
     9361 tgctgcccag gtagacagct tcagtcggca actgtcctga cgcgcgccag cagccccgca
     9421 agagcatgct gcgcgctttg ggtccgaacg ctggcgcggt cgcccgcgaa cagctggcgt
     9481 tcggtgctgc actggccgcc cacgcaccag ccgaaccaca ccgtgccgac cggcttgtct
     9541 tcgctccccc cggttggtcc ggcaacaccg gtgacggcga cgctgacgtg ggcggccgag
     9601 tgcgccacgg cgcctgcggc catggcgcgc accaccggct cgctgacggc gccgtgctgc
     9661 gcgatcagcg caggcggcac gcccagcagt tcggtttttg cggcgttgct gtaggtgacg
     9721 aagccgcgct cgaaccagtc ggacgagccg gccaggtcgg tgcaggcggc ggcgatcagg
     9781 ccgccagtgc agctttcggc ggtggccagc agccagccgc gctgacgcag cgcatcggcg
     9841 atgttttgaa tgatttcggc ttgtggcgct tgtccatcaa gcgccagcag ctcttttttt
     9901 tgaagcatgc ggttcaccat gcgcgccaca gcgccatcac cagcagcgtg cagaaagcag
     9961 ccaccaggtc gtcaagcagg atgccgaagc cgccgcgcca gccgtgcgcg ccgtgcagca
    10021 gccggtcggc ccagcccacc gggccgggct tgacggcgtc gaagaagcgg aacagcgcaa
    10081 aggccaccaa ctgcgtccaa aaacccgccg gcgtgaccag ccacaacacg atccagaagg
    10141 cgatcacttc atcgaccacg atggcgccgg ggtcggcgct ttgcaggtgg cgcgcggtga
    10201 cggtggcggc ccaccagccg gccagcagcg tgagcgcgat gacggcgccc tgcacggccg
    10261 gcgccagcgg ggccagcagc agcgcccaca ccagccaggc ccacagcgtg ccgacggtgc
    10321 cggcggccac aggcgacagg ccggcgccaa aggccagcgc gatccagtgc gccgggtggc
    10381 cgcgcatgaa ggcggcggtg ggtcgcaccg cgccagcggg tggcggaccg tcgccgccgg
    10441 caatggattc cagttcgacg ggccgcgatc cgccatcacg cctgatgtcg cgggcggcgc
    10501 ccagcggccg cgcctgcacg tcggtcacgt cgtcttgcgc gtggcctgcc aggctcactg
    10561 cctttcgcgc agccgcgcgt ccttcggcca ggtgatcacg tgctcggcag aaaagcgctg
    10621 catggcgccg gcggcgagcg gctgctccca catcacgctg ccgggctgtt cgttccaggc
    10681 cttggtctgc ggctcgggct gatagcgcga ttcgacgcgc acgtcctcgt gctcggccac
    10741 cggggcggcg tccagcagtt gcagcgtgac ggcctcgcgg tggcggtttt ctgccgtgta
    10801 gaggcggttc acgcggcgct cgttgcggct gccgatgaag ccgccagtgc cttctttttg
    10861 cgccggctgc tccatgcgca ccgtcaccag ttcgtcgcgg ccaaaggcca aaccattgcg
    10921 cgccagttgc gcggtgtcga agcggccatt gccgacgtag gcgccgtcgc ggtacaggct
    10981 gatcgggccg gccggccaca cgccgggcgg tggggcgatg gcggcgatca ggtaagcgct
    11041 ggcgtccagc gccggcgtgg cgcgcaccag caggcgcgcg tccagcgttt gctcaccaag
    11101 ggacagcgtg atgcgctggc cgctggaggg cacgctgatc ttctgcggca cggcaaattc
    11161 agtggcaaag ctgccttgga acacgctgac atcgaagctg ggcatggctt cggcggactc
    11221 ggccgtggtg gcgcgcatgc gcgccaccgg tgccggcgcg ggcatgggcg cgccggccat
    11281 cgcggcaacc ggggcctgcg gcggtggcgg cggctcgatg ccgatgcgcc acggccttgg
    11341 cagcggccct tgcgtggcgg cgccgggctg gccggtggag agggtgagct gcacgccgtc
    11401 ccaatcctcg ccggtgtttt gcgccaccag cgcctggcgc tccaaccgca ctttcttgct
    11461 cgcgctgtcg agcgtggcgc ggtagctggg ctgccagccg gggccgcgca cctgatagct
    11521 caaacgcagc tcgccgccct gcggcgcggc cagcgtgacc tgcaccgtgc tgaccagcgc
    11581 cttttgcgtg ccggcgcggt cgcgctcggc caccagcggc ttcagctcgg cttccagcgc
    11641 ctcttgctgg cgcttgatct ggtgctggcg cgtcagcgca tcgcgcgccg actggcgcag
    11701 cgcctgcgcg gtggcgccga tctgcgcggg cgcggtgcgg ctgtcggcgc cgctggcggc
    11761 gttctcgaag ctcttgaaat agcccgccgc ataaccgatg ccggcgcttt cggccgtcag
    11821 cgcggcgatc tggtcttcca gcgcgccgat gcggtcttcc agcgggttgg cgcaggcctt
    11881 gccgagcaac tcgcgcgcct gctggcgcac cgccagttcg cccacgcgca cgttggtgtc
    11941 gctggcgacc tgcaggctgg ccgcgtccag cccggccggc aggcaggcga aggtggcctg
    12001 gcgcgcgccg ggagccacgc gcaacacgcg ctccacggtg gcgctgccgg ggtagacctt
    12061 gacgcggatc acgcgcgagg cgtcggctcg cgtctcggtc gattgcgcct gcgcgggcga
    12121 cagcgcgcac agggccagca tcggcaacag caggcgccag tgcggccctg aatgggcaag
    12181 agtcttgagg tgcatggcct gcatgctagc agcgcggtgg gtggcttcgt gtttttgaat
    12241 caaacaggcg cctggcgctt gtccatcaag cgcaagcagc tatgaattcg ggagtgattg
    12301 ggtgccggat gagcgcggct gccgcgtggc ttcctgcgct gccgcgatgg cgccgtggat
    12361 tgccgtccgc gcctgcgggc tgttgttcca gcagctcgag cccgccaatg ccgcgccgcg
    12421 cgccagaacg tcggccgagt tggcgcgcgc caggtgcgcc agcgactcga tgcccatctg
    12481 ctccagccgc gcgatcacgg tggggccgac gcccttgacg gccagcagcg cctggcgctc
    12541 gctcggcgcg aaaggcatca gccggtggcg gcctgcagct tggccgcggc ttcgtcgtcg
    12601 atggcgttgg cgcgcaacgc ggcggggcgc tgggcgtgca gttcggcaaa gcgcacgaag
    12661 tcggggcgcg gctccagcat cttgaactgc aggtaaaagc cgatcgacgc agccacaaac
    12721 agatcggcag cggtgaagtg atcgcccgcc agatggtcac gccctttcag cgcgccttcg
    12781 agcgtggcga gcacgtcggc ttcggagccg tagcccgcct gttcgggctt ggccagcgcg
    12841 ccggcgctct tggcggtgag catcgcttcc agcggcgcca tgaaggcgat ccagcggtag
    12901 taggggccgc gcgccggcgt gcccacgggc ggcgccagct ttttctcggg caccagatcg
    12961 gccaggtaca gcaggatggc ggcgttctcc gtcaccacgg tgtcgccgtg ctggatggcc
    13021 ggcaccttgc ccat
//
LOCUS       DAOMVO010000034        12680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1125_length_12680_cov_16.685307, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000034 DAOMVO010000000
VERSION     DAOMVO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_12680_cov_16.685307"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      147..388
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            618..1295
                     /locus_tag="PKV30_03185"
     CDS             618..1295
                     /locus_tag="PKV30_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRL29257.1"
                     /translation="MHIEPGLVDGAKLALSYTTAAACIGYTAKLSADAIRKDGAAALL
                     LRAALATALVFCFFEVLPHHPVGVSEVHLILGTTLMLLFGAAPAAIGLAGGLLIQGLF
                     FAPFDLPQYGMNVTTLLVPLFATAALARRVIPDQMAYVDLRYGQALKLSLAYQGGIVL
                     WVGFWALYGRGLGAENLVSIGSFGAAYMSVVLAEPLIDLAVLALAKTLRGLQGSAVVD
                     RRLHQAA"
     gene            1309..2091
                     /gene="cobM"
                     /locus_tag="PKV30_03190"
     CDS             1309..2091
                     /gene="cobM"
                     /locus_tag="PKV30_03190"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HRL29258.1"
                     /translation="MGLGTVWFVGAGPGDPDLVTVKGRDLIARAGAILFAGSLVNEAA
                     TRWAPPGCLIADSKDMTLEQMSAWLIANAARCDTVVRLQTGDPALYGTLIELVQPLDA
                     AGVPVAVVPGVSSAMASAAAAVESFTLPEVTQTTIFTRVEGRTPMPDGEDLAALAAHR
                     TTLCIFLSITLLHKVEAGLRAAGWSDDAPVLVVHKASWPGEERIVRGTLATIKQQCRD
                     AGIVSQAMVIASPTLGARHWPELTRSRLYDPGFTHRFRKATT"
     gene            2088..2978
                     /locus_tag="PKV30_03195"
     CDS             2088..2978
                     /locus_tag="PKV30_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HRL29259.1"
                     /translation="MNHTTILLVGHGSREDTGNQEIRVFVDQWRARQPAWRIEVCFIE
                     FAAPSLHDGLLAAARSSRRVLVLPLILNAAGHAKMEIPEAIEHAREHAPGVEFLYGPH
                     LSACDPVLAILKRRLRQAMATLDMPDPTTTGVVLLGRGSSDRGANGDMAKMARWLQEE
                     SDHELVDLAFTGITFPRLERVVQRQVLIGLRQVVVLPYYLYTGTLMQRIHRQVDHLRA
                     QYPQIRFACGTHFGFEKEIFELVEQRVHDLLAGVPDSKLPCDGCSYRELAHDMGHGHS
                     HEHTHAPVHVHSHAHEGAAA"
     gene            2975..3655
                     /locus_tag="PKV30_03200"
     CDS             2975..3655
                     /locus_tag="PKV30_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HRL29260.1"
                     /translation="MSTVNTVTEQLTAAGQAIEHDSFAIIDAEAGRHAYSAEQWPIVR
                     RMIHANADFEFNGLTDFHPGAVRAGVAAMLRGGTPVVADVEMICSGLSRPRLAHFGMS
                     THQFISDADVIAQAQAQNTTRAVQAMRKAHRLGLLQGAVVGIGNAPTALIELVRLIME
                     DGVRPALVVGMPVGFVSAAESKDLMAQIADVPWIVIRGRKGGSTLVVAALHALLALAE
                     AQQRAEGA"
     gene            3656..4855
                     /locus_tag="PKV30_03205"
     CDS             3656..4855
                     /locus_tag="PKV30_03205"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HRL29261.1"
                     /translation="MESTTPRGTRTGFTTGACAAAAARAAAQGLETGQVPDAMESLLP
                     NGQRVRFDVHDRRCDAHSAHAMVVKFAGDDPDCTDGAHLTVDLRLLPGKAGEIAFVAG
                     DGVGTVTLPGLGLAVGGPAINPVPRQNIADNLREAAPRLLTQHGLEVTVSVPGGQEMA
                     KKTTNARLGILGGISILGTTGIVKPYSTAAWRASVVQGIQVAATLDHGVVALTTGGRT
                     ETFAMQTLRAERPGLPAACFVQMGDFLRYALDEVVAQGLPEVVLGVMVGKLTKIAQGE
                     TITHANRNVVDTDLVAEIARQIGATDEDCAAIAAAETARFGAELMVERGLGPAFHQAL
                     AQRAIDTLTAPERYGHAFALRVLVCDFSGKLVADVRSAPVEARPRPATAGRTGIGHTH
                     HFDFDTE"
     gene            4852..6207
                     /locus_tag="PKV30_03210"
     CDS             4852..6207
                     /locus_tag="PKV30_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cobalt-precorrin-7
                     (C(5))-methyltransferase/cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HRL29262.1"
                     /translation="MNDLPSHDPNRCRVIGVLDDGVASLGATALAHLRTADVVIGGAR
                     TLALFRHALKPGTLTHDLTGQLKAVPGWVRAAREAHQSCVVLATGDPLCHGIAPYLAQ
                     HLCLDALEILPNLSTLQLACARVGLAWQDARIVSVHAADAGEWQRGAPPSHGLYALAQ
                     ALRAEQRLLLLTSPANCPDRIARLLLAEGLGSDFHMAVAEDLLQPDEQVHAQLSPTEA
                     ASMRFAELNVVLLWRTRPRPQPVRFGLADSAFEQRQPEKGLITKQEVRAVSLARLQLH
                     ADSVVWDIGAGSGSVGLEAARLCPQGHVFAIEKNEADHAIARRNHAAFGVSNYTLQHG
                     KAPEGLEDWPDPDAVFIGGSGGELAGLIEQVLRRLRPGGRLVMNFVTLENLATATQAL
                     QQLNADWDVLQLQAARSRPILHMHRMAAENPVWLVCAGVGPMGHGEHGPVVAAEETAH
                     G"
     gene            6200..7018
                     /gene="cobI"
                     /locus_tag="PKV30_03215"
     CDS             6200..7018
                     /gene="cobI"
                     /locus_tag="PKV30_03215"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HRL29263.1"
                     /translation="MADVTPSTTPRMGTLHGISLGPGDPGLITRAAWAALECRDAIWV
                     YPVRSGKTPSYALDIVMRAGLPLPETHTPLLFPMTHDGEKLARAWLKAAQTVLPWLQA
                     GREVLFLVEGDASTYATFGHLARTLRGLDERVPVRTIAGVNSFTAACADVGEPFAEQD
                     DTVAIVPAAYGVGAVDRLLPDFDTLVLMKIKPLIEDLLDWLEARDLLAGAHFVERVGA
                     LDERRLSGPELLTLRGSKVSYLSLLIVKNPHRVRGERIKGCLKKSGPPPLETTP"
     gene            7015..7794
                     /locus_tag="PKV30_03220"
     CDS             7015..7794
                     /locus_tag="PKV30_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis central domain-containing
                     protein"
                     /protein_id="HRL29264.1"
                     /translation="MNPDITAPPRVCLVAITKHGSAQAATLARQLPQASLCVAAKFAE
                     ATRGLSNPVQAYEGAFKDQIGSLFQAFDQIVFFVSLGAVVRLIAPHLKSKDEDPGVLV
                     VDDAGQFVIPVLSGHVGGANAMAETVAALLGATAVLTTASDVGKTIPVDILGRELGWQ
                     VEAPKLNITRVSAAVVNEEPVAVVQEAGSPHWWTRPTPLPPNIHRHTRLEDVDLARYR
                     AVLWITHRPVDDTLWQTLHERLVVYRPPLPAGNDATTEAAA"
     gene            7791..8915
                     /gene="bluB"
                     /locus_tag="PKV30_03225"
     CDS             7791..8915
                     /gene="bluB"
                     /locus_tag="PKV30_03225"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HRL29265.1"
                     /translation="MKVALGIGCDRGTPLATLARAVDEALAVAGLQRGQVTGVGSITL
                     KADEPALLALAAQAGWPLHFYPATQLAEVVVPHPSETVRRHTGTPSVSEAAALLAAGT
                     TEASALCVEKHRLRGGDGRNATVSVARVMPRTAIPPCVANPDPARRFTASEREAVQAL
                     MQVRRDMRHFTAGAQVAEDVRARLQSALLAAPSVGLMQPLRVLRITDPVLRDRLAAAV
                     DQERMRTAQALGPRAAEFLALRVEGVRECAELWALVLAPDDGTLFGRRTLASEMAWCS
                     AGAAVQNLWLAARAEHLGLGWVSLFEPGDLHTLLALPAGAVPLGLLCIGPVDRFYETP
                     LLIQQQWRQARPVPALFTENNWAPSWNTDTSATTEQPLHS"
     gene            8959..9978
                     /gene="cobJ"
                     /locus_tag="PKV30_03230"
     CDS             8959..9978
                     /gene="cobJ"
                     /locus_tag="PKV30_03230"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HRL29266.1"
                     /translation="MTTDTPVTAPRNAGTTGRISLVGIGPGHTDHMTARARAAIAEAD
                     VVVGYVTYIKLVADLLDGKEVVRKGMTEELDRAVHALAAARAGKKVALISSGDAGVYG
                     MAGPTYEVLFQAGWTPEDHVAVEIVPGASAINACAALVGAPLTHDFCSISLSDLLTPW
                     PVIARRLDAAAAADFVVALYNPKSGRRTRQIVEAQRLFLRHRRADTPVAIVKSAYRRR
                     QHIVHTTLDQMAEQEIGMLSTVLIGNSSTFLREGLMVTPRGYANKYDVDQGFETREGE
                     KAGRSLSSGLLGWIEGLRAEHAAGAGLVELASRHRLPADYIAQVLAAPDAHAPAEAGT
                     EETEA"
     gene            9975..10325
                     /locus_tag="PKV30_03235"
     CDS             9975..10325
                     /locus_tag="PKV30_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRL29267.1"
                     /translation="MSSTEKPRIGDYRRHLLVCTGTRCTQDGAAQVLFDSLGEKFKAA
                     GLNEGALRVKRSRVSCFAACKGGPILCVQPDGTWYHGVTPDNMDRIIGQHLVGGQVVA
                     DLVFHQGPGVIDGE"
     gene            10315..11061
                     /gene="cobA"
                     /locus_tag="PKV30_03240"
     CDS             10315..11061
                     /gene="cobA"
                     /locus_tag="PKV30_03240"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRL29268.1"
                     /translation="MVSETPAAGSVCIVGAGPGPADLMTLRALNRLKQAQVVVHDRLI
                     AEDVLALIPAAARRVYVGKALGHHAVPQEEIHDLLVGHARQGLRVVRLKGGDPYVFGR
                     GGEEVLALQAAGIAFEVVPGVTAASGCSAAAGIPLTHRHLAASCVFLPGHLADDQASH
                     DWQALAGAGQTRVFYMGVQRLAQIAQALIAHGLPPDTPAAIVQDGARPTQSVLAHPLR
                     RLVEIAPPYGPRPGLLIIGETVRLSPSFEE"
     gene            11535..12446
                     /locus_tag="PKV30_03245"
     CDS             11535..12446
                     /locus_tag="PKV30_03245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL29269.1"
                     /translation="MDKPFISLSPEITRANALTLMDWLEDEGVTRYLSDSSHVSRFIE
                     QVIHRVQLPILTHLFNQGGRFFMAYDRHDVPVGFVRLVKNGRDCEMVLVIGNRDNWGR
                     KLGASAIREGMKLAFFDMRADKLIAKIHPDNERSLKAFLRSGFSLERETPAMKSLAMN
                     SERYLRLLRESPAAHTCDIYITEIDKARLRSLVEVEHDSGIFELEHEIERAIVVDPRQ
                     VAQDVVTMNSKALLRVDDEELEVALVYPEDADDGAGKLSVCSGIGTAILGYKEGDAFS
                     WRMPHRTCHIRIGKVLYQPEAAGDFHL"
BASE COUNT         2008 a         4467 c         4274 g         1931 t
ORIGIN      
        1 ctaagcaagc acttcagctt caggagtgca gcattggagt gcggagtgtc agccaatacg
       61 agcctctcgc ctgtgctcca tgctccgatg cacgagctct aggattgcaa cgttgccgct
      121 caaacgctcc gagcaatttt tgggggtata gaataaaagc tgttggtgct cgcgatgctg
      181 ttcaggcatc gcagttcaac gggaagcagg agggcgagtc ccacacggac gaacctaacc
      241 tgcgctgccc ccgcaacggt aagcagacgg gtggtccaag gcattgccaa ggcccccatt
      301 tctcaaaatt tcgccactgg gtcacaccgg gaaggctttg agaaacgttc tgtcagcccg
      361 gataccggcc aacaaggagg ctgcgcccca agcgcagccg cattacccaa acaccgtcgg
      421 ggaaggcggt gaggggtctt cgccggtgtt ctcatgtcgt cccttcgcat tgctttgcga
      481 gtctgtgcca gcgcctgcgc gtgcgctcgc cgggggctgt ccgttcgccg acggaacctg
      541 tcctcacctc tcccttgggt ctttgccgac tttttaaccg cgttgtgtcc aacgcatcca
      601 acccaaggag tcacttcatg cacatcgaac caggtctggt ggacggggcc aagctcgccc
      661 tgagctacac cacggcggcc gcctgcatcg gctacacggc caagctgtcc gcggacgcga
      721 tcagaaagga cggcgcagcg gccctgctgt tacgggccgc gctggccacg gcgctggtgt
      781 tctgcttttt cgaggtgctg ccgcaccacc cggtgggcgt gtccgaggtg cacctgatcc
      841 tgggcaccac gctgatgctg ctgttcggcg cggcacccgc ggccatcggg ctggcgggtg
      901 ggttgctgat ccagggcctg ttctttgcac ccttcgacct gccgcagtac ggtatgaacg
      961 tgaccacgct gctggtgccg ctgttcgcca ccgcggcgct ggctcgccgc gtgattcccg
     1021 accagatggc ctatgtggat ctgcgctacg gccaggccct gaagctgtcg ctggcctacc
     1081 agggcggcat cgtgctgtgg gtgggtttct gggcgctcta cgggcgtggc ctgggcgccg
     1141 agaacctggt gagcatcggc agcttcggcg ccgcctacat gagcgtggtg ctggccgagc
     1201 cgctgatcga tctggccgtg ctcgcactgg ccaagacgct gcgtggcctg cagggcagcg
     1261 cggtggtgga tcgccgtctg caccaggccg cctgaccggg ggcacaccat gggcctcgga
     1321 acggtctggt ttgtgggggc gggtccgggc gacccggacc tggtcacggt caaggggcgc
     1381 gacctgatcg cgcgcgccgg ggccatcctg ttcgccggtt cgctggtgaa cgaggcggcc
     1441 acccgctggg caccgccggg ttgcctgatc gcggacagca aggacatgac gctggagcag
     1501 atgtcggcct ggctgatcgc aaacgcggcg cgctgcgaca ccgtggtgcg cctgcaaacc
     1561 ggcgacccgg ccctctacgg cacgctcatc gaactggtgc agccgctgga cgccgcgggc
     1621 gtgccggtgg cggtggtgcc gggcgtgtct tcggccatgg cctcggcggc ggcggcggtc
     1681 gagagtttca ccttgcccga ggtcacgcag accaccatct tcacgcgggt ggaaggccgc
     1741 acgcccatgc ccgacggcga ggacctcgcc gcgctggcgg cgcaccgcac gaccttgtgc
     1801 atctttctgt ccatcacgct gctgcacaag gtggaagccg gcctgcgcgc cgccggctgg
     1861 tccgacgacg cgccggtgct cgtggtgcac aaggccagct ggcccggcga agagcgcatc
     1921 gtgcgcggca cgctggccac catcaaacaa cagtgccgcg acgctggcat cgtcagccag
     1981 gccatggtga tcgccagccc cacgctgggc gcgcgccact ggcccgagct gacccggtcc
     2041 cggctctacg acccgggctt cacacaccgc ttcaggaaag ccacgacatg aatcacacca
     2101 cgatcctgct ggtgggccac ggctcgcgcg aggacaccgg caaccaggaa atccgcgtct
     2161 tcgtcgacca gtggcgcgcg cgccagccgg cctggcgcat cgaggtctgc ttcatcgaat
     2221 ttgccgcgcc ctcgctgcac gatggcctgc tggccgcggc gcggtcgtcg cgccgcgtgc
     2281 tggtgctgcc actgatcctg aatgccgccg gtcacgccaa gatggagatt cccgaggcca
     2341 tcgagcacgc ccgcgagcac gcacccggcg tcgagttcct ctacggcccg cacctgagcg
     2401 cctgcgaccc cgtcctcgcc atcctcaagc gccgcctgcg ccaggccatg gccacgctgg
     2461 acatgcccga ccccaccacc accggcgtgg tgctgctggg ccggggctcg tccgaccgtg
     2521 gcgccaacgg cgacatggcc aagatggcgc gctggctgca ggaagaaagc gaccacgagc
     2581 tggtggacct ggccttcacc ggcatcacct ttccgcggct ggagcgcgtg gtgcagcggc
     2641 aggtgctgat cggcctgcgc caagtggtgg tgctgcccta ctacctctac accggcacgc
     2701 tgatgcagcg catccaccgc caggtggacc acctgcgggc gcagtacccg cagatccgct
     2761 ttgcctgcgg cacgcacttc gggttcgaga aagaaatctt cgaactggtg gagcagcgcg
     2821 tgcacgacct gctggccggt gtgcccgaca gcaagctgcc ctgcgacggc tgcagctacc
     2881 gcgagctcgc acacgacatg ggccatgggc actcccacga gcacacgcac gccccggtgc
     2941 acgtgcacag ccatgcgcac gagggtgctg ccgcatgagc acggtcaaca ccgtcaccga
     3001 gcagctcacc gccgccggcc aggccatcga gcacgacagc ttcgccatca tcgacgccga
     3061 ggccgggcgg cacgcctact cggccgagca gtggccgatc gtgcgccgca tgatccacgc
     3121 caacgccgac ttcgagttca acggcctgac cgacttccac cccggcgcgg tgcgtgcggg
     3181 cgtggccgcg atgctgcgcg gaggcacccc cgtggtggcc gatgtggaga tgatctgttc
     3241 gggcctgtcg cggccgcgcc tggcgcactt cggcatgtcc acgcaccagt tcatcagcga
     3301 cgccgacgtg attgcgcagg cgcaggcgca gaacaccacc cgcgccgtgc aggccatgcg
     3361 caaggcacac cgcctgggcc tgctgcaggg cgccgtcgtc ggcatcggca acgcgcccac
     3421 cgcgctgatc gaactcgtgc ggctgatcat ggaggacggc gtgcgcccgg ccctggtggt
     3481 gggcatgccg gtgggtttcg tgtcggccgc agagtcgaaa gacctgatgg cgcagatcgc
     3541 cgacgtgccc tggatcgtca tccgcggccg caagggcggc tccaccctgg tggtcgccgc
     3601 cctgcacgcg ctgctggcgc tggccgaggc acagcaacgc gccgagggcg cctgaatgga
     3661 aagcaccacc ccgcgcggta cacgaaccgg cttcaccacc ggcgcctgcg cggccgcggc
     3721 cgcccgggcc gcggcgcagg ggctggaaac cgggcaggtg cccgacgcca tggagagcct
     3781 gctgcccaac ggccagcggg tgcgttttga cgtgcacgac aggcgctgcg atgcgcacag
     3841 cgcccacgcc atggtggtga agttcgcggg cgacgacccg gactgcaccg acggcgcgca
     3901 cctcacggtg gacctgcgcc tgctgcccgg caaggcaggt gagatcgcgt tcgtggccgg
     3961 cgacggcgtg ggcacggtga cgctgcctgg cctcgggctg gcggtgggcg gtccggccat
     4021 caatccggtg ccgcgccaga acatcgccga caacctgcgc gaggccgcgc cgaggctact
     4081 cacacagcac gggctggagg tcaccgtgag cgtgcccggc ggccaggaga tggcgaagaa
     4141 gaccaccaac gcgcggctgg gcatcctggg cggcatcagc atcctgggca ccaccggcat
     4201 cgtcaaaccc tattccaccg ccgcctggcg ggccagcgtg gtgcagggca tccaggtggc
     4261 ggccacgctg gaccacggtg tggtggcgct gaccaccggc ggccgcaccg agacatttgc
     4321 catgcagacg ctgcgtgcgg aacgccctgg cctgcccgcc gcctgcttcg tgcagatggg
     4381 cgacttcctg cgctacgcgt tggatgaggt ggtggcccag ggcctgcccg aggtggtgct
     4441 gggcgtgatg gtgggcaagc tgaccaagat cgcccagggc gagaccatca cccacgccaa
     4501 ccgcaacgtg gtggacaccg acctggtcgc cgagatcgcc cggcagatcg gcgccacgga
     4561 cgaagactgc gccgccatcg ccgccgccga gaccgcgcgc ttcggcgccg agctgatggt
     4621 cgaacgcggc ctgggcccgg cctttcacca ggcgctcgca cagcgcgcca tcgacacact
     4681 caccgcgccc gagcgctacg gccacgcgtt tgcgctgcgc gtgctggtgt gcgacttcag
     4741 cggcaagctg gtggccgacg tgcgctcggc ccccgtcgag gcgcgccccc gccccgccac
     4801 cgccggccgc accggcatcg gccacaccca ccacttcgac ttcgacaccg aatgaacgac
     4861 ctgccctccc acgatcccaa ccgctgccgc gtcatcggcg tgctcgacga cggcgtggcc
     4921 agcctcggcg ccaccgcgct ggcccacctg cgcacggccg acgtggtgat tggcggcgcg
     4981 cgcacgctgg cgctgttccg ccacgcactc aagcccggca ccctcaccca cgacctgaca
     5041 ggacaattga aggccgtgcc cggctgggtg cgcgccgcac gcgaggccca ccagtcctgc
     5101 gtggtgctgg ccaccggcga cccgctgtgc cacggcatcg caccctacct tgcccagcac
     5161 ctgtgcctgg acgcgctgga gatcctgccc aacctctcga ccctgcaact ggcctgtgcc
     5221 cgcgtgggcc tggcctggca ggacgcacgc atcgtctccg tgcacgccgc cgacgcaggc
     5281 gagtggcagc gcggtgcccc accctcgcac ggcctgtacg cgctggccca ggccctgcgc
     5341 gccgagcagc gcctgctgct gctgaccagc ccggccaact gccccgaccg catcgcccgc
     5401 ctgctgctgg ccgaaggtct gggcagcgac ttccacatgg ccgtggctga agacctgctg
     5461 cagcccgacg aacaggtgca tgcgcagctc agtcccaccg aggcggccag catgcgcttt
     5521 gccgagctga acgtggtgct gctgtggcgc acccggccgc gaccgcaacc ggtgcgcttc
     5581 ggcctggccg acagcgcctt cgagcagcgc cagcccgaga aaggcctgat caccaagcag
     5641 gaggtgcgcg ccgtcagcct ggcgcggctg cagctgcacg ccgacagcgt ggtctgggac
     5701 atcggcgccg gcagcggttc ggtgggcctg gaggccgccc gcctgtgccc gcagggccat
     5761 gtgttcgcca tcgagaagaa cgaggccgac cacgccatcg cccggcgcaa ccatgcagct
     5821 ttcggcgtca gcaactacac gctgcagcac ggcaaggcgc ccgagggcct ggaggactgg
     5881 cccgaccccg acgcagtgtt catcggtggc tcgggcggcg agctggccgg actgatcgag
     5941 caggtgttgc ggcgcctgcg gcccggcggc cggctggtga tgaacttcgt gacactggag
     6001 aacctggcca ccgccaccca ggccctgcag cagctgaacg ccgactggga cgtgctgcag
     6061 ctgcaggcgg cgcgcagccg ccccatcctg cacatgcacc gcatggcggc cgagaacccg
     6121 gtgtggctgg tgtgcgccgg cgtgggcccc atgggccacg gcgaacacgg gccggtggtg
     6181 gcggccgagg agacggcgca tggctgacgt cacgccttca accaccccgc gcatgggcac
     6241 cctgcacggc atctcgctgg gcccgggcga ccccggcctg atcacccgcg cggcctgggc
     6301 cgcgctggag tgccgcgacg ccatctgggt ctacccggtg cgcagtggca agacgcccag
     6361 ctatgcgctg gacatcgtga tgcgcgccgg gctgccgttg cccgaaacgc acacgccgct
     6421 gctgtttccc atgacccacg acggcgagaa gctggcgcgc gcctggctga aggccgcgca
     6481 gaccgtgctg ccctggctgc aggccgggcg cgaggtgctg ttcctggtcg agggcgatgc
     6541 ctccacctac gccaccttcg gccacctcgc gcgcaccctg cgcgggctgg acgagcgggt
     6601 gccggtgcgc accatcgcag gagtcaattc gttcaccgcg gcctgtgcgg atgtgggcga
     6661 accgttcgcc gagcaggacg acacagtggc catcgtgccg gcggcctacg gcgtgggcgc
     6721 ggtcgaccgg ctgctgcccg acttcgacac cctggtgctg atgaagatca agccgctgat
     6781 cgaggacctg ctggactggc tggaggcgcg cgacctgctg gccggcgcgc atttcgtgga
     6841 gcgtgtgggc gcgctggacg agcgccggct gtccggcccc gaactgctca cgctgcgcgg
     6901 cagcaaggtc agctacctct cgctgctgat cgtgaagaac cctcatcgcg tgcgcggcga
     6961 gcgcatcaag ggctgcctga aaaagagcgg cccaccgcca ctggagacca ccccatgaac
     7021 ccggacatca ccgcccctcc ccgcgtctgc ctggtcgcca tcacgaaaca cggctctgcc
     7081 caggcggcca cgctggcgcg gcagctgcct caggcgtcgc tctgtgtcgc ggccaagttt
     7141 gcagaagcca caaggggcct gtccaacccg gtgcaggcgt acgaaggcgc gttcaaggac
     7201 cagatcgggt cgctcttcca ggccttcgac cagatcgtgt tcttcgtctc actgggtgcg
     7261 gtggtgcggc tgatcgcacc ccacctgaag agcaaggacg aggaccccgg cgtgctggtg
     7321 gtggatgacg ccggccagtt cgtcattccc gtgctctcgg gccacgtggg cggcgccaac
     7381 gccatggccg agaccgtggc cgcgctgctg ggcgccacgg cggtgctcac caccgcgtcg
     7441 gacgtgggca agaccatccc ggtcgacatc ctgggccgtg agctgggctg gcaggttgag
     7501 gcgcccaaac tcaacatcac ccgcgtctcg gccgcggtgg tcaacgagga accggtggcc
     7561 gtggtgcagg aggccggcag cccgcactgg tggacccgcc ccaccccgct gccgccgaac
     7621 atccaccgcc acacgcgcct ggaggatgtg gatctggccc gctaccgcgc ggtgctgtgg
     7681 atcacccacc gcccggtgga cgacacgctc tggcagacgc tgcacgagcg gctggtggtg
     7741 taccgcccgc cgctgccggc cggcaacgac gccaccaccg aggccgcggc atgaaggtgg
     7801 ccctgggcat cggctgcgac cgcggcacgc ccctggctac gctggcgcga gcggtcgacg
     7861 aagccctggc cgtggccggc cttcagcgcg gacaggtgac cggcgtgggc agcatcacgc
     7921 tcaaggccga cgaacccgcg ctgctggcgc tggccgcgca ggccggctgg ccgctgcact
     7981 tctaccccgc cacccagctg gccgaggtgg tggtgcccca cccgtccgaa accgtgcgcc
     8041 gccacaccgg caccccttcg gtctccgaag ccgcggcgct gctggccgcg ggcaccaccg
     8101 aggcctccgc cctgtgcgtc gagaagcacc ggctgcgcgg cggcgacggg cgcaacgcca
     8161 cggtgtcggt ggcgcgggtg atgccgcgca cagccatccc gccctgtgtc gccaaccccg
     8221 atccggcgcg ccgtttcaca gccagcgaac gcgaagccgt gcaggcgctg atgcaggtgc
     8281 ggcgcgacat gcgccacttc actgccggtg cgcaggtcgc cgaagacgtg cgggcccgcc
     8341 tgcaatcggc gctgctcgcc gcgccgtccg tgggcctgat gcagccgctg cgggtgttgc
     8401 gcatcaccga ccctgtattg cgcgaccggc tggccgctgc cgtggaccag gagcgcatgc
     8461 gcaccgcgca ggccctgggc ccccgtgcgg ctgagttcct ggcactgagg gtggaagggg
     8521 tgcgcgagtg cgccgaactg tgggcgctgg tgctggcgcc agacgacggc acgctgttcg
     8581 gtcgccgcac gctggcgagc gaaatggcgt ggtgctcggc gggcgccgcc gtgcaaaacc
     8641 tctggctggc cgcgcgcgcc gaacacctgg gtctcggatg ggtgtcgctg ttcgaacccg
     8701 gcgacctgca cacgctgctg gcgctgcccg cgggcgccgt gccgctgggc ctgttgtgca
     8761 tcggtccggt ggatcgcttc tacgaaacac ccctgctcat ccagcaacag tggcgccagg
     8821 cgcgtccggt gccggccctg ttcaccgaga acaactgggc cccgtcctgg aacaccgaca
     8881 ccagcgccac caccgaacaa ccgctgcaca gctgaccgcg gccttgccat tgaacccccg
     8941 cccgtcccaa ggaacgacat gaccacagac acccccgtca ctgccccgcg caacgcgggc
     9001 accaccggcc gcatcagcct ggtgggcatc ggccccggcc ataccgacca catgaccgcc
     9061 cgcgcgcgcg ccgccatcgc cgaggccgac gtggtggttg gctacgtcac ctacatcaag
     9121 ctggtggccg acctgctgga cggcaaggaa gtcgtgcgca agggcatgac cgaagagctg
     9181 gaccgcgccg tgcacgcgct ggccgcggcc cgcgccggca agaaggtggc gctgatctcg
     9241 tcgggcgacg ccggcgtcta cggcatggcc ggcccgacct acgaggtgct gttccaggcc
     9301 ggctggacgc cggaggacca tgtggcggtg gagatcgtgc ccggcgcgtc ggccatcaac
     9361 gcctgcgcgg ccctggtcgg cgcgccgctg acgcacgact tctgctccat ctcgctgtcc
     9421 gacctgctga cgccctggcc ggtgatcgcc cggcgcctgg acgccgccgc cgcggccgac
     9481 ttcgtggtgg cgctttacaa cccaaagagc gggcgccgca cgcgccagat cgtcgaggcg
     9541 cagcgcctgt tcctgcgcca ccgccgggcg gacacgccgg tggcgatcgt caagagcgct
     9601 taccggcgcc gccagcacat cgtgcacacc accctggacc agatggccga acaggagatc
     9661 ggcatgctca gcaccgtgct gatcggcaac agcagcacct tcctgcgcga ggggctgatg
     9721 gtcaccccgc gcggttacgc caacaaatac gacgtggacc agggcttcga gacccgcgaa
     9781 ggcgagaagg ccggccgctc gctctccagt ggcctgctgg gctggatcga ggggctgcgg
     9841 gccgagcacg cagccggcgc gggactggtc gaactcgcca gccgacaccg cctgcccgcc
     9901 gactacatcg cacaggtgct ggcggcgccc gatgcacacg caccggccga ggccggcaca
     9961 gaggagaccg aggcatgagc agcacagaaa aaccccgcat cggcgactac cgccggcacc
    10021 tgctggtctg caccggcacg cgctgcacgc aggacggcgc ggcgcaggtg ctgttcgaca
    10081 gcctgggcga gaagttcaag gccgccgggc tgaacgaagg cgcgcttcgc gtcaagcgca
    10141 gccgcgtgag ctgttttgct gcctgcaagg gcggccccat cctgtgcgtg cagcccgacg
    10201 gcacctggta ccacggcgtg acccccgaca acatggaccg catcatcggg cagcacctgg
    10261 tgggcggaca ggtagtcgcc gatctggtgt tccaccaggg accgggggtc atcgatggtg
    10321 agtgagacgc cggctgcggg ctcggtctgc atcgttggcg ccgggccggg accggccgac
    10381 ctgatgacgc tgcgcgcgct gaaccggctg aaacaggccc aagtggtggt gcacgatcgg
    10441 ctgatcgccg aggatgtgct ggcgctgatc ccggccgccg cccggcgtgt gtacgtgggc
    10501 aaggcgctgg gacaccacgc ggtcccgcag gaagagattc atgacctgct ggtggggcat
    10561 gcgcgccagg gcctgcgcgt cgttcgcctc aaaggtggtg acccctatgt gttcggacgt
    10621 ggtggcgagg aagtgctggc gctgcaggcg gccggcattg cattcgaggt ggtgcccggt
    10681 gtgacagccg ccagcggctg ctccgccgcg gctggcattc cgctgactca ccgccacctt
    10741 gcagccagct gcgtgtttct gcccggccat ctggcggacg accaggccag tcacgactgg
    10801 caagccctgg cgggtgcggg gcagacccgg gtgttctaca tgggcgttca gcgtctggcg
    10861 cagattgccc aggccttgat cgcccacggg ctgccgcccg acacacccgc ggccatcgtc
    10921 caggacggtg cccgacccac acagtccgtt ctggcccacc cattgcgccg cttggtcgag
    10981 atcgctcccc cctacggtcc tcgaccgggc ttgctgatca ttggcgaaac ggtgcgactg
    11041 agcccgtcat ttgaagaatg atgcgagtgt ctgagtcgac ggcaagggcg ctgactctcc
    11101 ttggagctga tcgctgctgc ctggcgcagc agacaatttg gggattcgga cacatttccg
    11161 gcagccaacg ctagtgttgc actgcttctc gaagttagcg gtgtcccgtg cgtcccctac
    11221 tcagtgcttg tcctgcagga ggacccggcg ttcgatccgc aatcggttca gcgtcagctg
    11281 tgtgtgtatc agcgatcatt gagtcgtaca ccgaaggtga cggcagtcag cctcaaccag
    11341 taattcgccc catgaattcg agagagggca actggccgca acaggcctct ccaaatcccc
    11401 cgatacacgg gttcaacaaa gccccgcctc cccccccgca ctcgcacggc gccttaaaat
    11461 ccgccttctg ccgaaagaac cgtcatggcc gatgccatga agggctttca tacttgaaac
    11521 ccactggggt gaagatggac aagcctttca tttctctgag tcccgagatc actcgggcca
    11581 acgcgctgac tttgatggac tggttggaag acgagggcgt gacgcgctac ctgagcgatt
    11641 cgagccatgt ctcccggttc attgagcagg tcatccatcg ggtccagtta cccatcctga
    11701 cccatctgtt caaccagggc ggccggttct tcatggccta tgaccgacat gatgtgccag
    11761 tgggctttgt gcgtctcgtc aagaacggcc gggactgcga aatggtgctg gtcatcggca
    11821 accgcgacaa ctggggccgc aagctcggag ccagcgcgat ccgcgaaggc atgaagctcg
    11881 ccttcttcga catgcgggcc gacaagctga tcgccaaaat ccacccggac aacgagcgtt
    11941 cgctgaaggc cttcctgcgc agcggcttct cgctcgagcg cgaaacgcct gcgatgaagt
    12001 ctttggccat gaactcggag cggtatctgc ggctcttgcg cgaaagccct gcggcgcaca
    12061 cctgtgacat ctacatcacc gaaatcgaca aagcccgact caggagtctg gtcgaagtcg
    12121 aacacgattc gggaatattc gagctggagc acgagatcga gcgcgccatc gtcgtcgatc
    12181 cgcgccaagt ggcacaggat gtcgtcacga tgaactccaa ggccctgctg cgggtggacg
    12241 acgaggaact agaggttgcg ctggtctacc cggaggatgc ggacgacggt gccgggaaac
    12301 tatcggtgtg ctccggcatc ggcaccgcga tcctgggcta caaggagggc gatgccttca
    12361 gctggcggat gccgcaccgc acctgccata tccggattgg gaaagtgctt tatcagcccg
    12421 aagccgcggg cgatttccac ctgtaggttc acctctaggc ttgttcacac gctgcacgat
    12481 ctgcaccact gctgcagatc gtgcgggagc gctcgggtcg gccgtgtgtg attcggccgc
    12541 caaggtgact tcgtcggacg cctccgcaga gcggtcattg aatgcccaaa gtcagaacgg
    12601 cgctgagtcg gggtcggtgt ccactggcgc ttccctgggc ccggtcacca cgccaccgcc
    12661 ggcacttttg cgcgcgggtg
//
LOCUS       DAOMVO010000035        12621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1135_length_12621_cov_16.684466, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000035 DAOMVO010000000
VERSION     DAOMVO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_12621_cov_16.684466"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2230)
                     /gene="carB"
                     /locus_tag="PKV30_03250"
     CDS             complement(<1..2230)
                     /gene="carB"
                     /locus_tag="PKV30_03250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRL29270.1"
                     /translation="MPKRTDLQSILIIGAGPIVIGQACEFDYSGVQACKALREEGYRV
                     ILINSNPATIMTDPATADVTYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WRNGVLAKYNVELIGAKPEAIDKAEDRLKFKDAMTRIGLGSARSGIAHSMDEAWAVQK
                     KVGFPTVIRPSFTLGGTGGGIAYNPEEFETICKRGLEASPTNELLIEESLLGWKEFEM
                     EVVRDKADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQVMRNASIAVLREIG
                     VDTGGSNVQFAVNPADGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNEITGGATPASFEPTIDYVVTKIPRFAFEKFPAADSHLTTQMKSVGEVMAMGRTFQ
                     ESFQKALRGLEVGVDGMNEKTQDREWLEKELGEPGPDRIWFVGDAFAAGWSLDEVHDL
                     TKIDKWFLVQIEEIIKIELDIDKHTAEHGDKALEALSKQELLTLKQKGFSDRRLAKLL
                     KTSDKAVRERRRALNVRPVYKRVDTCAAEFATNTAYMYSTYEHECEAEPTSNKKIMVL
                     GGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIVDKEKPTGVIVQYGGQTPLKLALGLEAEGVPIIGTSPDMIDAAEDRERFQKL
                     LHELGLRQPPNATARTEGEALEKAAELGYPLVVRPSYVLGGRAMEIVHEQRDLERYMR
                     EAV"
     gene            complement(2241..2897)
                     /locus_tag="PKV30_03255"
     CDS             complement(2241..2897)
                     /locus_tag="PKV30_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29271.1"
                     /translation="MPEPRAPRASRSQRCPRWSRRGVTALALLAAGCAQPPQTPFDRV
                     QRFADAYVRLLPVGRVMEVAAAQDARWPLGAKADLVSDTQLACMRAGLSSSVVSGRQR
                     QAARDYAQAHADTLDGDLRVLEAGAARLIGDAMLAGAGDPAPRLPTSATPEETQALAA
                     FATEPRYAALRRATGLDALLGGGTERAAQRGRDLGHALVVKAMTDAFLSCHIPVKLLY
                     "
     gene            complement(2890..3252)
                     /locus_tag="PKV30_03260"
     CDS             complement(2890..3252)
                     /locus_tag="PKV30_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29272.1"
                     /translation="MKLYSFPQAALEKAIAKRMLTLASPHKEWFADRWSQKPYKKSFI
                     EHKAMPLVTLLAKGKTWTDEEFNGELAAWGAKFYDAEAEVLRPMVEGDGVIQLMQKNM
                     PPERVQALLNKLEQDRHA"
     gene            complement(3249..4460)
                     /gene="carA"
                     /locus_tag="PKV30_03265"
     CDS             complement(3249..4460)
                     /gene="carA"
                     /locus_tag="PKV30_03265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRL29273.1"
                     /translation="MLASLEGKFPPAILALADGSVFLGQSIGATGTTVGEVVFNTSMT
                     GYQEILTDPSYCQQIVTLTYPHIGNVGANDEDIESGKVQAAGLIIKDLSALASNFRSQ
                     RTLSDYLKDGGTVAIAGIDTRKLTRILRTQGAQNGAIVALAAGEQATPDRIDAAVQAA
                     RQAPPMVGLDLAKVVSTDQPYEWDQTEWALGTGYGKLAEPRFHVVAYDFGVKKNILRM
                     LAARGCRVTVVPAQSTAAEVLAQRPDGVFLSNGPGDPEPCDYAIAATREIVGSGVPVF
                     GICLGHQIMALAAGAKTYKMKFGHHGANHPVKDLQDGRVSITSQNHGFAVDADTLPSH
                     LRATHVSLFDHTLQGFAFVDQPAFCFQGHPEALPGPHDVGYLFDRFIRNMELAHASQA
                     QAATKTGVNAA"
     gene            complement(4624..5619)
                     /locus_tag="PKV30_03270"
     CDS             complement(4624..5619)
                     /locus_tag="PKV30_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29274.1"
                     /translation="MPSTPLSRRDVLQAGVAALAALPLAARAQAAWPSKPVRLVVPFA
                     PGGSSEIVARAVAAEMAKTLGQNVFVENKPGAAGNIAMQEVANSTDEHTIILGHIGTL
                     AVNPYIFPKLPYDANRDFAPITLVSKVPSLYVVHPDLPVKNIKEFVALAKAKPGQLNY
                     GSAGNGSAGHLAMEYLKMVSNTFMLHVPYRGTGPMLTDLMAGRLQAASVGAPALLSFI
                     KAGKLRCIGTGTGQRLPQLPDVPTVAEQGFAGFEMTQWYGLLAPRKWAPAHLAKLEAE
                     AVKATRGALVKDKLAQETALAVGNSSAEFAQFIATEQKRWSQVIARAQIKPEGMG"
     gene            complement(5693..6469)
                     /locus_tag="PKV30_03275"
     CDS             complement(5693..6469)
                     /locus_tag="PKV30_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRL29275.1"
                     /translation="MSESGPQRITSRDNALLKDLRRLAQDSGAHRKQGRMWVEGEHLC
                     SAALVRGLKPVLAVFSASYWPLAQAGQAQAALKNVVIDDALMASISDLPSPAPLAFVL
                     DLPAAPALQAEAPTVVLDRVQDAGNVGSILRSAAAFGFTQIVALKGTALLWSPKVLRA
                     GMGAHFGLRLVEGLAAHDLSALQVPLLVTSSHEGEWLHQASLPWPCAWVMGHEGQGVS
                     PELAARAAMALRIAQPGGEESLNVAAAAAICLCTSAVARV"
     gene            complement(6466..7101)
                     /gene="rnhB"
                     /locus_tag="PKV30_03280"
     CDS             complement(6466..7101)
                     /gene="rnhB"
                     /locus_tag="PKV30_03280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRL29276.1"
                     /translation="MRSRRFLRAEQAPLDWDSPGLIAGVDEAGRGPLAGPVVAAAVIL
                     NPAKRIRGLADSKVLSPQKRERLHDEIMDKALCCAIALATVQEIDTLNILQATLLAMR
                     RAVEGLRLPPALVMVDGNRLPVLPMRAEAVIDGDAKVKAISAASILAKVHRDRWCAEI
                     DAHWPQYGFAAHKGYGTAAHLAALREHGACEHHRRSFAPVAAVFVRQGESA"
     gene            complement(7073..8284)
                     /gene="lpxB"
                     /locus_tag="PKV30_03285"
     CDS             complement(7073..8284)
                     /gene="lpxB"
                     /locus_tag="PKV30_03285"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRL29277.1"
                     /translation="MERAAPFFGLVAGEASGDLLAGLLLDGLRARWPQLTTAGIGGAH
                     MTAQGFDAWWPIEKLSVRGYLEVLPRLPELLSIRKQLARRLLTERPDLFIGVDAPDFN
                     FDLEARLKKAGIKTLHFVSPSFWAWRPEKLLKLKAAADHVLCLFPFEPELLAEHGIAA
                     TYVGHPLANVIPMQPDRAAARRVLGLDENATVVALLPGSRAAEVKYLLSGFFKAAALI
                     RQARPATQFIVPAVPHLLTQIEAAARVSGLGDALKVVRGQSHAVLAACDVTLIASGTA
                     TLEAALFKRPMVIAYAMPAFSYRLMRKKRQLPWVGLPNVLCAPREWLHRPAGMSAHDM
                     ADRTDAPFVVPELIQDAATPAALAQAVLDWLAAPEKIAQLQQRFTALHHELKRDTPRL
                     ATDAIETLLAR"
     gene            complement(8296..9084)
                     /gene="lpxA"
                     /locus_tag="PKV30_03290"
     CDS             complement(8296..9084)
                     /gene="lpxA"
                     /locus_tag="PKV30_03290"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRL29278.1"
                     /translation="MSGIHATAVVDPRAELDASVSVGPYAVIGPHVRVGAGTTIGPHC
                     VIEGHTRIGSDNRIFQFASIGAAPQDKKYAGEPTRLEIGHGNTIREFVTINTGTVQDE
                     GVTRLGDDNWIMAYVHIAHDCQLGSHIILANAVQLAGHVHLGDWVFLGGLTGVHQFGR
                     VGAHAMTAFQTRLAQDVPPFVTAGGNPAEAQSINAEGLKRRGFSPERITLIKQMYRLL
                     YRKGLTLDAARQQIDALRGEVAEADADIVLMQGFLSSAARGIVR"
     gene            complement(9091..9534)
                     /gene="fabZ"
                     /locus_tag="PKV30_03295"
     CDS             complement(9091..9534)
                     /gene="fabZ"
                     /locus_tag="PKV30_03295"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRL29279.1"
                     /translation="MMDIHKILTKLPHRYPFLLVDRVLDIDMQAKTIRAIKNITVNEP
                     CFTGHFPLRPVFPGVLMLEALAQACALLSFETEGIALDDESVFYFVGIDGARFKRPVE
                     PGDQLILHSALERARGGIYKFNCHADVDGDVAAEAQIMCTMRKVV"
     gene            complement(9596..10597)
                     /gene="lpxD"
                     /locus_tag="PKV30_03300"
     CDS             complement(9596..10597)
                     /gene="lpxD"
                     /locus_tag="PKV30_03300"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRL29280.1"
                     /translation="MTLRTGAIVQALGGELQGDPDRLITQLAPLERAQPSELSFLVNP
                     RLAPLLKTTQAGCVIVGAESAEAARQRGGDGAGDCIVADNPHLYFARVTQLWKRLHGG
                     AARRGIHPSAVVEEGAFVDPTASVGALCFIGRGACIGADTVLKERVTVGEDCIIGARC
                     VLQSGAVIGGDGFGFAPNQGAWEKIEQLGAVRIGDDVEIGANTCIDRGALDDTVIEDG
                     VKLDNLIQIGHNCHIGQHTAMAGCVGVAGSARIGAYCTVGGAGMILGHLTIADHVHIS
                     SASIVTRSIHKPGHYTGFFPIAENGEWEKNAATLKQIYRLRDRVKHLEEFLPGQDQE"
     gene            complement(10653..11144)
                     /locus_tag="PKV30_03305"
     CDS             complement(10653..11144)
                     /locus_tag="PKV30_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRL29281.1"
                     /translation="MVVAVLAAVVGAPAAIAQENARIGFVNTDRMLREAAPAKTAQTK
                     LEQEFSRREKEIDDAGATLKTASERFEREAPTLSESQRQNRQRQLMDQDREFQRKRRE
                     FQEDLNSRKQEELQQILDRANRVVKQVAEAEKYDVILQEAVYVNPRLDITDKVIKALN
                     AGK"
     gene            complement(11168..>12621)
                     /gene="bamA"
                     /locus_tag="PKV30_03310"
     CDS             complement(11168..>12621)
                     /gene="bamA"
                     /locus_tag="PKV30_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRL29282.1"
                     /translation="GNYLDKDDEFKSLIKIKPGEAYNADDVAETTKAFTEHFGNYGYA
                     FAQVEAVPEVDRVNNRVAITLRSEPSRRAYVRRINIQGNTRTRDEVIRREFRQYESSW
                     YDAEKIRLSRDRVDRLGYFTELSVSSAEVPGAPDQVDLNVEVKEKPTGSISIGAGYSS
                     AEKLAFTFGLSQDNVFGSGNKLDLQLNTGKFNRTIVVSATDPYFTANGVSRTVDVYYR
                     TQKPYQDQGGNYTLITMGAGLKFGVPVTELDTVYLGINAERTRIKPGTNIPAAYLDYA
                     NRFGYTSSAFPFTLGWSRDSRDSALAPNSGRYQRLLGELGLFGDARYVKANYQYQQFV
                     PLSKQYTLAFNGEVGWGKGLSGQPFPVFKNFYAGGLGSVRGYEQSTLGPRDVTGAYIG
                     GTRKFVLNTEFITPFPGAGNDRTLRLFGFVDVGALYGDGEKISGDALRYSAGIGLSWI
                     SPLGPLRFAIAQPLRKRPGDRIQRFQFQIGTSF"
BASE COUNT         2006 a         4090 c         4241 g         2284 t
ORIGIN      
        1 tcaccgcctc gcgcatgtag cgctccaggt cgcgctgctc gtgcacgatt tccatggcgc
       61 ggccgcccag cacgtagctg gggcgcacga ccagcgggta acccagttcg gcggcttttt
      121 ccagcgcttc gccttcggtg cgggcggtgg cgttgggcgg ctggcgcagg cccagctcgt
      181 gcagcagttt ctgaaagcgc tcgcggtctt cggcggcgtc gatcatgtcg gggctggtgc
      241 cgatgatggg cacgccttcg gcttccaggc ccagcgccag cttcaacggc gtctggccgc
      301 cgtactgcac gatgacgccg gtgggctttt ccttgtcgac gatttccagc acgtcttcga
      361 gcgtcagcgg ctcgaagtac aggcggtctg acgtgtcgta atcggtagaa accgtctcgg
      421 gattgcagtt gaccatgatg gtttcgtaac catcctcgcg catggcgagg gccgcgtgca
      481 cgcagcaata gtcgaactcg atgccctggc caatgcggtt ggggccaccg cccagcacca
      541 tgattttctt gttgctggtc ggctcggcct cgcattcgtg ctcgtaggtc gagtacatgt
      601 aggcggtgtt ggtggcgaac tcggcggcgc aggtgtccac gcgcttgtac acggggcgca
      661 cgttcagcgc acggcggcgt tcgcgcacgg ccttgtcgct ggttttcagc agtttcgcga
      721 ggcgacggtc tgaaaagcct ttttgcttca gtgtcagaag ctcttgtttt gagagcgcct
      781 ccaacgcctt gtcgccatgt tcggcggtgt gcttgtcgat gtcgagttcg atcttgatga
      841 tctcctcgat ctgcacaaga aaccacttgt cgatcttcgt caggtcgtgc acctcgtcca
      901 gcgaccagcc ggcggcaaac gcatcgccca cgaaccagat gcggtccggg ccgggttcgc
      961 ccagttcttt ttcaagccat tcgcggtctt gcgtcttctc gttcatgccg tcgacgccga
     1021 cttccagccc gcgcagcgct ttctgaaacg attcctgaaa cgtgcggccc atcgccatga
     1081 cctcgcccac gctcttcatc tgcgtggtca gatgactgtc ggcggcggga aatttctcaa
     1141 acgcaaagcg cgggatcttg gtgaccacgt agtcgatggt cggctcgaac gacgccggcg
     1201 tggcgccgcc cgtgatctcg ttgcgcagtt catccagcgt gtagcccacg gccagcttgg
     1261 cggccacctt ggcgatcgga aaacccgtgg ccttggacgc cagcgctgat gagcgcgaca
     1321 cgcgcgggtt catctcgatc acgatcatgc ggccatcggc cgggttgacg gcgaactgca
     1381 cgttggaacc gccggtgtcg acgccgatct cgcgcagcac ggcgatgctg gcgttgcgca
     1441 tgacctggta ttccttgtcg gtcagcgtct gcgcgggcgc caccgtgatc gaatcgcccg
     1501 tgtgcacgcc catgggatca aggttttcga tcgagcagat gatgatgcag ttgtcggcct
     1561 tgtcgcgcac gacttccatc tcgaactctt tccagccgag cagcgattcc tcgatcagca
     1621 actcgttggt gggcgaggct tccaggccgc gcttgcagat ggtctcgaat tcttccgggt
     1681 tgtaggcaat gccgccgccc gtgccgccaa gcgtgaagct gggtctgatg acggtgggaa
     1741 agcccacttt cttctgcacg gcccaggctt cgtccatgct gtgggcgatg ccgctgcgcg
     1801 ccgacccgag gccgatgcgc gtcatcgcgt ccttgaactt caggcggtct tcggccttgt
     1861 cgatggcctc gggcttggcg ccgatcagct cgacgttgta cttggccagc acgccgttgc
     1921 gccacagatc gagcgcgcag ttcagcgccg tctggccacc catggtgggc aggatggcgt
     1981 cggggcgctc cttggcgatg attttctcga ccgtctgcca ggtgatcggc tcgatgtagg
     2041 tcacgtcggc cgtcgccggg tcggtcatga tcgtggcggg gttgctgttg atcaggatga
     2101 cgcggtaacc ctcttcgcgc agcgccttgc aggcctgcac gccggagtag tcgaactcgc
     2161 acgcctggcc aatgacgatg gggccggcgc cgatgatgag aatgctttgg aggtcggtac
     2221 gtttgggcat atttcgagat tcaatacaac agcttgaccg ggatgtggca gctgagaaag
     2281 gcgtcggtca tggccttgac caccagcgca tggccaaggt cgcgaccgcg ctgggcagcg
     2341 cgttcggtgc cgccgccgag cagggcgtca aggccggtgg cgcggcgcaa ggccgcgtag
     2401 cgcggctcgg tggcaaaggc ggccagggcc tgggtttctt caggcgtcgc gctggtcggc
     2461 aagcgggggg ccgggtcgcc ggcgcccgcc agcatggcgt cgccaatcag gcgcgcggca
     2521 cccgcttcga gcacgcgcaa gtcgccgtcc agcgtgtcgg cgtgcgcctg ggcgtagtca
     2581 cgcgcggcct ggcgctggcg acccgacacc acggacgacg acagccccgc acgcatgcag
     2641 gccagttgcg tgtcgctcac cagatcggcc ttggcgccca gcggccagcg cgcatcctgc
     2701 gcggcggcga cctccatcac gcggccgacg ggcagcaggc gcacgtaggc gtcggcaaag
     2761 cgctgcacgc ggtcgaaggg cgtttgcggc ggctgcgcgc agcccgcggc cagcagggcc
     2821 agcgccgtca cgccgcggcg tgaccagcgc gggcatcgct gcgagcggga tgcgcgcggc
     2881 gcgcggggtt caggcatggc ggtcttgttc gagtttgttc agcaacgcct gcacgcgctc
     2941 gggcggcatg ttcttttgca tcagctggat cacgccatcg ccttcgacca tggggcgcag
     3001 cacctcggcc tcggcgtcgt agaacttggc gccccacgcg gccagttcgc cattgaattc
     3061 ttcatcggtc caggtcttgc ccttggccag cagcgtgacg agcggcatgg ccttgtgctc
     3121 gatgaaggac ttcttgtagg gcttttgcga ccagcggtcg gcaaaccact ccttgtgcgg
     3181 cgaggccaag gtcagcatgc gcttggcaat ggctttttca agcgcggcct ggggaaagct
     3241 gtagagcttc atgcggcgtt cactcctgtt ttggtagctg cttgcgcttg actggcgtgc
     3301 gccagctcca tatttcttat gaaacgatca aacaggtagc ccacatcgtg cgggcccggc
     3361 agcgcttcag gatggccctg aaagcagaac gcgggctggt cgacaaacgc aaagccctgc
     3421 agcgtgtgat cgaacaggct gacgtgcgtg gcgcgcaggt gcgacggcag cgtgtcggca
     3481 tccaccgcga agccgtggtt ctggctggtg atggacacgc ggccatcctg cagatccttc
     3541 accgggtggt tggcgccgtg gtggccgaac ttcatcttgt aggtcttggc acccgcggcc
     3601 agcgccatga tctggtggcc caggcaaatg ccgaacaccg gcacgccgct gcccacgatc
     3661 tcgcgcgtgg cggcgatggc gtagtcgcac ggctcggggt cgcccgggcc gttggagagg
     3721 aagacgccat cgggccgctg cgccaacacc tcggccgccg tgctctgcgc cggcaccacg
     3781 gtgacgcggc agccgcgcgc ggccagcatg cgcaggatgt tcttcttgac accgaagtca
     3841 taggcgacga cgtgaaagcg cggctcggcc agcttgccgt agccggtgcc cagcgcccac
     3901 tcggtctggt cccattcgta gggctggtcg gtcgacacca ccttggccag atcgagcccc
     3961 accatcggcg gcgcctggcg cgccgcttgc accgctgcat cgatgcgatc tggcgtggcc
     4021 tgctcacccg ccgccagcgc cacgatggcg ccgttttgcg cgccctgggt gcgcaggatg
     4081 cgcgtgagtt ttcgggtgtc gatgccggcg atggcgacgg tgccgccgtc cttcaggtaa
     4141 tcgctcagcg tgcgttgcga gcggaagttg ctggccaggg ccgacaagtc cttgatgatc
     4201 aggccggcgg cctggacctt gccggactcg atgtcctcat cgttggcacc gacgttgccg
     4261 atgtgcgggt aggtcagcgt gacgatttgc tggcagtagc tggggtcggt caggatttcc
     4321 tggtagccgg tcatcgaggt gttgaacacc acctcgccca cggtggtgcc ggtggcaccg
     4381 atggactggc ccagaaagac gctgccgtcg gcaagcgcca ggatggcggg cgggaatttc
     4441 ccctctaggg aagcaagcac tggggttctc cgtctggtgt cggtcgcccg tgcgcgtcgc
     4501 gcgccggcaa ggcacgcgga ctgctctcaa aaaaacgaag gggtgttttt ggcgcggtgg
     4561 ggcgacgcgg tttcgagaaa agcctccgat tataggcggt gccccacgcg cccgccggcg
     4621 cttttagccc atgccttcgg gcttgatctg cgcccgcgcg atgacctggc tccagcgctt
     4681 ttgctcggtg gcgatgaact gggcgaattc ggcgctggag ttgcccaccg ccagcgccgt
     4741 ctcctgcgcc agcttgtcct tgaccagcgc gccgcgcgtg gccttgacgg cttcggcctc
     4801 caatttggcc agatgcgccg gcgcccactt tctaggcgcc aggaggccgt accactgcgt
     4861 catctcgaag ccggcaaagc cctgctcggc caccgtgggc acatcgggca actgcggcag
     4921 ccgctgcccc gtgccggtgc caatgcagcg cagcttgccg gccttgatga aggacagcag
     4981 cgccggtgcg ccgaccgagg ccgcctgcag ccgccccgcc atcaggtcgg tcagcatcgg
     5041 cccggtgccg cgatacggca cgtgcagcat gaaggtgttg ctcaccatct tgaggtattc
     5101 catcgccagg tgccccgcgc tgccgttgcc ggccgagccg tagttgagct gcccaggctt
     5161 ggccttggcc agcgccacga actccttgat gtttttgacc ggcaaatccg ggtgcacgac
     5221 gtacaggctg ggcaccttgg agaccagcgt gatcggcgcg aagtcgcggt tggcgtcgta
     5281 cggcagcttc gggaagatgt aggggttgac ggccagcgtg ccgatgtggc cgaggatgat
     5341 ggtgtgctcg tcggtgctgt tcgccacctc ttgcatggcg atgttgccgg cggcgccggg
     5401 cttgttctcg acaaacacgt tctggccaag cgtcttggcc atttcggccg ccacggcgcg
     5461 ggccacgatt tctgaactgc cgcccggcgc aaacggcacc accagacgca ccggcttgct
     5521 gggccaggcg gcttgcgcgc gcgccgcgag cggcaaggcg gccagggcgg caacgccagc
     5581 ctgcagaacg tcgcggcgcg acaagggggt cgatggcatg agaggtctcc tcggtgtcgc
     5641 cgcaagatcg ggcgagcgaa ttatcgggat gcggcgcgcg gaagaaatcc gctcaaaccc
     5701 gcgccaccgc gctggtgcac aggcagatcg ccgccgccgc cgccacgttg agcgactcct
     5761 cgccgcccgg ctgtgcgatg cgcagcgcca tcgccgcgcg cgccgccagc tcgggcgaca
     5821 cgccctgccc ttcgtgaccc atcacccagg cgcagggcca cggaagcgac gcttggtgca
     5881 gccattcgcc ctcgtgcgag ctggtcacca gcaacggcac ctgcagcgcg gaaagatcat
     5941 gagccgccag cccctcgacc agccgcaacc cgaaatgcgc gcccatgccg gcgcgcagca
     6001 ccttgggcga ccacagcagc gccgtgcctt tcagcgccac gatttgcgtg aaaccgaacg
     6061 ccgccgcgct gcgcaagatg gagccgacgt tgcccgcgtc ctgcacacgg tcgagcacca
     6121 ccgtgggcgc ttcggcttgc agcgcgggcg ccgcaggcag atcaagcacg aaagccagcg
     6181 gcgcgggcga cggcagatcg ctgatgctgg ccatcagcgc atcgtcgatg actacatttt
     6241 taagagctgc ttgcgcttgt cctgcttggg ccagaggcca atacgatgca gaaaacaccg
     6301 ccagcaccgg cttcaaacca cgcaccagcg ccgcgctgca cagatgctcg ccctccaccc
     6361 acatccgccc ctgcttgcgg tgcgcgccac tgtcttgcgc cagccgacgc aagtccttca
     6421 gcagcgcgtt gtcgcgcgag gtgatgcgct gcggcccgga ttcgctcacg cgctttcgcc
     6481 ctggcgcacg aagaccgccg ccaccggcgc aaacgagcgg cggtggtgct cgcacgcgcc
     6541 gtgttcgcgc aacgccgcca ggtgcgcggc ggtgccgtaa cccttgtgcg cggcaaatcc
     6601 gtattgcggc cagtgcgcat cgatctcggc gcaccagcgg tcgcggtgca ccttggccag
     6661 gatcgacgcg gccgaaatgg ccttgacctt tgcgtcgcca tcaatcaccg cctcggcgcg
     6721 catcggcagc accggcaggc ggttgccgtc gaccatgacc agcgccggcg gcagacgcag
     6781 cccttcgacc gcgcgccgca tcgccagcag cgtggcctgc aggatgttga gcgtgtcgat
     6841 ctcctgcacc gtggccaagg cgatggcaca gcacagcgcc ttgtccatga tttcgtcgtg
     6901 caggcgctcg cgcttttgcg ggctgagcac cttggaatcg gcgaggcccc ggatgcgttt
     6961 ggccgggttc aggatgacgg cggcggccac caccgggccg gccagcgggc cgcgcccggc
     7021 ctcgtccacg ccggcgatca ggccgggtga atcccaatcc aggggtgctt gttcagcgcg
     7081 caagaagcgt ctcgatcgca tcggtggcca ggcggggcgt gtcgcgtttc agttcgtggt
     7141 gcagcgcggt aaagcgctgc tgcaattggg cgattttctc aggcgcggcg agccagtcca
     7201 gcacggcttg cgccagcgcg gcgggcgtgg cagcgtcctg gatcagctcg ggcacgacga
     7261 acggtgcatc ggtgcggtcg gccatgtcgt gcgcggacat gccggcgggg cgatgcagcc
     7321 actcgcgcgg cgcgcacagc acattgggca aacccaccca gggcagttgg cgcttcttgc
     7381 gcatcaaacg gtagctgaag gccggcatgg cgtaggcgat gaccatgggg cgcttgaaca
     7441 gtgcggcctc cagcgtggcg gtgccgctgg cgatcagcgt cacgtcgcag gcggcgagca
     7501 cggcgtgcga ctggccgcgc acgaccttca acgcatcgcc caggccgctc acgcgcgccg
     7561 cggcctcgat ctgagtcagc aggtgcggca ccgcgggcac tatgaattgc gtagctggtc
     7621 gcgcttgcct gatcagcgct gcggccttaa agaaacctga aagcagatat ttgacttcgg
     7681 ccgcgcggct gccgggcagc agcgccacca cggtcgcgtt ctcgtccagg cccagcacgc
     7741 ggcgcgcggc ggcgcggtcc ggctgcatgg gaatcacatt ggccaatgga tggccgacgt
     7801 aggtggccgc gatgccgtgt tcggccagca attcgggctc gaatgggaac aggcagagca
     7861 catgatcggc ggccgccttg agcttcagca acttctcggg ccgccaggcc caaaacgagg
     7921 ggctgacgaa atgcagcgtc ttgatgccgg ctttcttcag gcgcgcttcg aggtcgaagt
     7981 tgaaatcagg cgcgtcgacg ccgatgaaca aatcgggccg ttctgtcagc aggcggcgcg
     8041 ccagctgctt tcgaatcgat agcaactcgg gcaggcgcgg cagcacttcg agatagccgc
     8101 gtaccgagag cttttcgatc ggccaccagg cgtcaaagcc ctgcgccgtc atgtgcgcgc
     8161 cgccgatgcc ggcggtcgtc agttgcggcc agcgcgcccg caagccatcg agcagcaagc
     8221 cggccagcaa atcgcccgag gcttcgccgg cgaccagacc gaaaaacggc gcggcccgct
     8281 ccatgcgcac gtgcctcagc gcacgatgcc gcgcgcggcc gacgacagaa aaccctgcat
     8341 caatacgatg tccgcatccg cctcggccac ctcgccgcgc agcgcatcga tctgctggcg
     8401 cgcggcgtcc agcgtcaacc ctttgcggta cagcagccgg tacatctgct tgatgagcgt
     8461 gatgcgctcg ggcgaaaaac cgcggcgctt cagcccttcg gcgttgatgc tctgcgcttc
     8521 ggccggattg ccgccagcgg tgacgaaggg cggcacgtct tgcgccaagc gggtctgaaa
     8581 agccgtcatg gcgtgcgcgc cgacgcggcc gaactggtgc acgcccgtca ggccgcccag
     8641 aaacacccag tcgcccagat gcacgtggcc ggccagctgc acggcgttgg ccaggatgat
     8701 gtggctgccc aactggcaat cgtgcgcgat gtgcacgtag gccatgatcc agttgtcgtc
     8761 gcccagccgc gtgacgccct cgtcctgcac cgtgccggtg ttgatggtga cgaactcgcg
     8821 gatggtgttg ccgtggccaa tctccagccg agtcggctcg ccggcgtatt ttttgtcctg
     8881 cggcgcggcg ccgatgctgg cgaactggaa gatgcggttg tcgctgccga tgcgggtgtg
     8941 gccctcgatc acgcaatgcg ggccaatggt ggtgccggcg cccacgcgca cgtgcgggcc
     9001 gatgacggca tacgggccga cgctgacgct ggcgtccagc tcggcccgtg gatcaaccac
     9061 ggcggtggcg tgaatgccgc tcacggcgcc tcagacgact tttctcatgg tgcacatgat
     9121 ctgtgcttcg gcagcgacgt cgccgtcgac gtcggcgtgg cagttgaatt tgtagatgcc
     9181 tccgcgtgcg cgctccagcg ccgaatgcag gatgagttgg tcgcccggct ccacggggcg
     9241 cttgaagcgc gcaccgtcaa tgccgacgaa ataaaacacc gactcatcgt cgagcgcgat
     9301 gccctcggtc tcgaacgaca gcagcgcgca ggcctgcgcc agcgcctcca gcatcagcac
     9361 gcccggaaaa accggccgca gcggaaagtg cccggtaaag cagggctcgt tcaccgtgat
     9421 gttcttgatc gcgcggatcg tcttggcctg catgtcgatg tccagcaccc gatcaaccag
     9481 cagaaacggg tagcggtgcg gcagcttggt caggatcttg tggatgtcca tcatggcagt
     9541 ttgctcatca attaatagct gctcgcgctt gatggtcaag cgctggcggc ctgttttatt
     9601 cttgatcctg ccctggcaga aattcttcaa gatgcttgac gcggtcgcgc aggcggtaga
     9661 tctgcttgag cgtggccgcg ttcttctccc actcgccatt ctcagcgatg gggaagaagc
     9721 cggtgtaatg ccccggcttg tggatcgagc gcgtgacgat ggaagccgac gaaatgtgca
     9781 catgatcggc aatcgtcagg tgccccagga tcatgccggc gccgcccacc gtgcagtacg
     9841 cgccaatgcg cgcgctgcca gccacgccga cgcagccggc catcgccgtg tgctgaccga
     9901 tgtggcagtt gtggccgatc tggatcaggt tgtcgagctt gacgccgtct tcgatcaccg
     9961 tgtcgtcgag cgcgccgcgg tcgatgcagg tgttggcgcc gatctcgaca tcgtcgccaa
    10021 tgcgcacggc gccgagttgc tcgatcttct cccacgcgcc ctggttgggc gcaaagccga
    10081 agccgtcgcc gccgatgacc gcgccgctct gcagcacgca gcgcgcgccg atgatgcaat
    10141 cctcgccgac ggtgacgcgc tccttgagca cggtgtcggc gccgatgcaa gcgccgcggc
    10201 cgatgaagca gagcgcgccc acgctggccg tggggtcgac aaacgcccct tcttcgacca
    10261 ccgcgctggg gtggatgccg cgccgcgctg cgccgccgtg caggcgcttc cacagctgcg
    10321 tgacgcgggc aaagtaaaga tgcgggttgt cggccacgat gcagtcgccc gcaccatcac
    10381 cgccccgctg gcgcgcggcc tcggccgatt cggcaccgac gatgacacag ccggcttgcg
    10441 tggtcttgag caaaggcgcc agccgggggt tgaccaggaa gctgagttcg gacggctggg
    10501 cgcgctccag cggcgccagt tgggtgatca ggcggtcggg gtcaccctgc aactcaccac
    10561 ccaaggcctg aacgatggcg ccggttcgaa gggtcacgac tcgcgtctcg ggacgaagca
    10621 atgcaagtca ggcacggcgc gcgcggccga actcacttgc cggcgttcag cgccttgatc
    10681 accttgtcgg tgatgtcgag ccgcggattg acgtacaccg cctcttgcag gatgacgtcg
    10741 tacttctcgg cctcggccac ctgcttgacg acgcggttgg cgcggtcgag gatctgctgc
    10801 agctcttcct gcttgcgtga gttgaggtct tcctgaaatt cgcgccgctt gcgctggaac
    10861 tcgcggtcct ggtccatcaa ctggcgctgg cggttctggc gctggctttc actcagcgtc
    10921 ggcgcctcgc gctcgaagcg ctcggaagcc gtcttgagcg tggcgccagc gtcgtcgatt
    10981 tctttctcgc gccgcgaaaa ttcctgctcc agcttggttt gcgccgtctt ggcgggggcg
    11041 gcctcgcgca gcatgcggtc ggtgttgacg aagccgatgc gcgcgttctc ctgcgcaatt
    11101 gctgcgggcg cgcccaccac ggcggccagc accgcgacca ccatcagact gcgagagatg
    11161 aagggcttca aaacgaagtt ccgatctgga attggaaacg ctggattcta tcgccggggc
    11221 gcttgcgaag cggctgggca atggcaaagc gcagcgggcc caggggcgag atccagctga
    11281 ggccaatacc ggcggaatag cgcagcgcat cgccgctgat cttctcgcca tcgccgtaca
    11341 gcgcaccgac gtcgacaaag ccgaacagcc gcagcgtgcg gtcgttgccg gcacctggaa
    11401 aaggcgtgat gaactcggtg ttgagcacga acttgcgcgt gccgccgatg taggcgcccg
    11461 tcacgtcgcg cgggccgagc gtggattgct cgtagccgcg caccgacccg aggccaccgg
    11521 cgtagaagtt cttgaacacc gggaagggct ggccgctcag gcccttgccc caccccacct
    11581 cgccattgaa ggccagcgtg tactgtttgg acagcggcac aaactgctgg tattggtaat
    11641 tggccttgac gtagcgcgcg tcgccgaaca ggcccagctc gcccagcagg cgctggtagc
    11701 ggcccgagtt gggcgccagc gcgctgtcgc gcgagtcacg cgaccaaccc agcgtgaaag
    11761 ggaaggccga gctggtgtag ccaaagcggt tggcgtagtc gaggtaagcc gccgggatgt
    11821 tggtgccggg cttgatgcgc gtgcgctcgg cgttgatgcc gaggtacacg gtgtcgagct
    11881 cggtcacggg cacgccgaat ttcaggccgg cgcccatggt gatcagcgtg tagttgccgc
    11941 cctggtcctg gtatggcttc tgggtgcggt agtacacatc gaccgtgcgc gacacgccat
    12001 tggcggtgaa gtacgggtcg gtcgcgctga cgacgatggt gcggttgaac ttgccggtgt
    12061 tgagctgaag atccagcttg ttgccggagc caaacacgtt gtcttgcgac aagccgaagg
    12121 tgaaagccag cttctcggcc gatgaatatc cagcgccaat gctgatgctg ccggtaggtt
    12181 tctccttgac ctcgacattc aggtcgacct ggtcgggcgc gcccggcacc tccgcgctcg
    12241 acaccgacag ttcggtgaaa tagcccagcc gatccacacg gtcgcgcgag aggcgaatct
    12301 tctcggcgtc gtaccagctc gattcgtatt ggcggaactc gcggcggatg acttcgtcgc
    12361 gcgtgcgcgt gttgccctgg atgttgatgc ggcgcacata ggcgcggcgc gacggctcgg
    12421 agcgcagcgt gatggccacc cggttgttga cgcgatcgac ctcgggcacc gcctcgacct
    12481 gggcaaaggc atagccgtag ttgccaaagt gctcggtgaa ggccttggtg gtttcggcca
    12541 cgtcgtcggc gttgtaggcc tcgccgggct tgatcttgat gagcgacttg aactcgtcgt
    12601 ccttgtccag gtaattgccg t
//
LOCUS       DAOMVO010000036        12547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1142_length_12547_cov_16.445965, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000036 DAOMVO010000000
VERSION     DAOMVO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_12547_cov_16.445965"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1029)
                     /locus_tag="PKV30_03315"
     CDS             complement(244..1029)
                     /locus_tag="PKV30_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL29283.1"
                     /translation="MSQTLRIATTGHVATVVLNRPEVRNAFNDEVIAELSQAFVQLGE
                     ASEVRAIVLMAEGPAFCAGADLNWMRRMADYTRDENIEDAGKLAFMLRAIYECPKPTI
                     ARVQGDVYAGGMGLVAACDMAVSVDTANYCLSEVKLGLIPATISPYVIRAMGARAAHR
                     YFLTAERFGAAEALRIGFVHEVVSAEALDAKVGELVSALTSASPNAVRECKKLVQDVA
                     ERDITRLLIERTVEGIADIRASDEGKEGVQSFLQKRKPAWLAA"
     gene            complement(1026..1538)
                     /locus_tag="PKV30_03320"
     CDS             complement(1026..1538)
                     /locus_tag="PKV30_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRL29284.1"
                     /translation="MDARAHFAALARYNAWATQRLLDAVAAVNDTDYRRDTGLFFKSI
                     HGTLNHLLVGEHLLWFRRFAEGVSPMVTLDEEVEVDRSLLAERLLEGAARWQPLIENW
                     PSEGFDGVLSYTTTRGVPVSLPFAATLTHVFNHGTHHRGQITAAFTRLGQPFPELDLV
                     YMLQQEQTRP"
     gene            complement(1541..1942)
                     /locus_tag="PKV30_03325"
     CDS             complement(1541..1942)
                     /locus_tag="PKV30_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRL29285.1"
                     /translation="MLVVDSSVWIDFFNARDTAARVELRRLLDDGNTRLVIPDLVLFE
                     VLRGFRYERPLRQARQLLQTLSVEPCGGEDVALAAAEHYRYLRKRGVTVRSAVDVLIA
                     AFCIENDYLLLHSDSDYDGFAAHRGLRVWGH"
     gene            complement(1929..2282)
                     /locus_tag="PKV30_03330"
     CDS             complement(1929..2282)
                     /locus_tag="PKV30_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRL29286.1"
                     /translation="MRTNIDIDDALLAKAMQAGPYSTKKETVEAGLQLLARQAAYREI
                     LKWEGKLKWEGDDAHDWVALDAQDQAQPQHETEALAVQEPAVSYRVNRSTDPASAKAP
                     AAKPRKTGTKTHVGR"
     gene            complement(2322..3944)
                     /locus_tag="PKV30_03335"
     CDS             complement(2322..3944)
                     /locus_tag="PKV30_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL29287.1"
                     /translation="MSVIETKLNARSADFQANAAAMQALVDDLQAQVQKVGAGGGDAA
                     RKKHTDRGKLLPRVRVQMLLDPGTPFLEVAPLAALGMYPDRDGGDSAPGAGIVAGIGR
                     VSGIDCMIVCNDATVKGGTYYPLTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDE
                     VFPDRDHFGRIFYNQANMSAQGIAQIAVVMGSCTAGGAYVPAMSDETIIVKNQGTIFL
                     GGPPLVKAATGEVVSAEDLGGGDVHTRLSGVADHLAHNDMHALALAREAVSRLNAKKV
                     YSGSLQAPAAPKYAAEELYGVIPTDTRKPFDVREIIARIVDNSELHEFKSRYGTTLVC
                     GFAHIEGMPVGIVANNGILFAESARKGAHFIELCCQRKIPLVFLQNITGFMVGRKYEN
                     EGIARDGAKMVTAVATANVPKFTIIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVM
                     GGEQAAGVLATVKRDGIEAKGGSWSAEEEAAFKAPIKEQYEVQGHPYYATARLWDDGV
                     IDPADTRRVLALGLAAAQNAPVPDVKFGVFRM"
     gene            complement(3997..5718)
                     /locus_tag="PKV30_03340"
     CDS             complement(3997..5718)
                     /locus_tag="PKV30_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29288.1"
                     /translation="MVSASPTAAPSHARGATEPPLIEQTIGALFDAMVERYPEREALV
                     VAHQQRRYTYRQLQTEANRLASALLGLGLSPGDRVGIWSHNNAEWVLMQLATAKAGLV
                     LVNINPAYRTAEVEYALNKVDCKALVTMPRFKTSDYIGMLRELAPEWAHAKAGEPLES
                     QTLPHLKHAIWIDVAGEGADEPGFMRFSDLIQRGDPADERVARIARLLHPRDPINVQF
                     TSGTTGFPKGATLTHRNILNNGFFIGEAMKLTHEDRLCIPVPLYHCFGMVLGNLAVLT
                     HGGCIVYPNDAFDPLTVLQTVQAEKCTGLHGVPTMFIAELDHPDFAKYDLSTLRTGIM
                     AGSPCPVEVMKRVQADMNMAEVTIAYGMTETSPVSCQSSTDTPLDKRVSTVGLVQPHL
                     EIKIIDPDSGQTVPRGATGELCTKGYSVMQGYWGDDARTREAIDAEGFMHTGDLATMD
                     DEGYVNIVGRIKDMVIRGGENVYPREIEEFLYRHPQIQDVQVVGVPDQKFGEELCAWI
                     IAKPGQTLDEAGVREFCKGKIAHYKVPRYLKFVDAFPMTITGKIQKFRIRELMQEELG
                     LSEQKTA"
     gene            5839..6864
                     /locus_tag="PKV30_03345"
     CDS             5839..6864
                     /locus_tag="PKV30_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL29289.1"
                     /translation="MPRDATTTGQTPIAFVRAITGAYAARGLDPSAALQKARIAPADL
                     ARSRARVTAVQFERLCAAAMQELDDEAPGWFSRRLPWGSYGMLARASISAPTLGVALA
                     RWCRHHGLLTDDVTLTLGQAGGQARVEIAEHRDLGALREFALLSLLRNLHGLACWLVD
                     SHIALQGAQFPLPAPPHADMYTRLFPGPVQFGAPRAGLQFDAHYLQLPLRRDGAALDQ
                     MLRRALPILVWPYRRDRLLSRRVRRLLQQPHAAHTAETLATRLGLSARSLHRQLQGEG
                     TSLQALKDEVRRERASDLLLRTQWPIARVARAAGFTSDKSFARAFRQWTGHTPQTHRQ
                     RGSPLES"
     gene            6960..8381
                     /locus_tag="PKV30_03350"
     CDS             6960..8381
                     /locus_tag="PKV30_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRL29290.1"
                     /translation="MPQPTIPDALWRLPASQTAALVRAHQVSATEVARAALARVDAVN
                     PRLNAIVECRPDEVLARAAAIDAALARGEDPGPLAGVPITTKVNVDQAGYATTNGLKS
                     QKDLIATEDAPMVRGLMADGAVLLGRTNVPAFCYRWFTSNQLHGRTLNPRNAALTPGG
                     SSGGAASAVAAGLGAIAHGTDIAGSVRYPAYACGVHGLRPSLGRVANFNATAPKDRSI
                     GGQIMAVSGPLARTVDDLRLGLAAMARPDTRDPWYAPVPLSGPDLPRRAAVCLRPDGM
                     ATAPEICAALLDSADRLRDAGWVVDEVDALPPLQEAVPLLITLWMGDGYDAMVQAAEA
                     EGDPGAIAALAGQAEFARGIGLPQYSDALVRRATLARAWQRFLAERYSAVLLPLSAEL
                     PFEDDLDLKDAASYQRVWSAQMSQIALPLTGLPALALTTGNVGNGVPVGIQIVAARFR
                     EDVCLGVAEAIEARLPALAPALP"
     gene            complement(8426..8824)
                     /locus_tag="PKV30_03355"
     CDS             complement(8426..8824)
                     /locus_tag="PKV30_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HRL29291.1"
                     /translation="MTSYRLLQGLTGVLALIPILTGAITMMGVHDPLYAALDLPGNPL
                     LASNLRFLGGVWLGLGLAAFWMVPRLHAQTVLFCVVWGAIFIGGLGRLLSMLVVGWPP
                     APFIAFTVLEIVGAPLFVGWHARVVNPLPR"
     gene            8967..10124
                     /locus_tag="PKV30_03360"
     CDS             8967..10124
                     /locus_tag="PKV30_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL29292.1"
                     /translation="MHTQARPLFTAEHDAFREQVRRFCAQEIAPHHAAWEDAHGVPRA
                     LWQHAGANGLLCCWLPEALGGPGADLLFDFIVAEELGRIGATGPGFPLHSVIVAPYLA
                     AHGTPALQQALLPAMVAGEKIAAIAMTEPGTGSDVAAIRTQARRDGEHYVLSGQKTFI
                     TNGHNADVVVVACKTEPDQGARGVSLLVVERGMPGFTRGRNLRKIGQHAQDTAELFFE
                     EVRVPASHLLGEEGQGFKYLMQKLAQERLLVSVGCQARAEAALQWTVDYVKERKAFRQ
                     RVADFQNTRFKLAAVATDITAGRAYCDTLIAQHLAGELDAVGAAAGKLWHSELLGRVT
                     DECLQLFGGYGYMADYPIARAYADARIERIYAGTSEIMKEIVARDLLDAPR"
     gene            complement(10311..>12547)
                     /locus_tag="PKV30_03365"
     CDS             complement(10311..>12547)
                     /locus_tag="PKV30_03365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL29293.1"
                     /translation="SQSGALTASILDWARQNHVGFSAVVALGAHTQVGLADVLDFLAN
                     DRRTQGIVVYMEGIANARRFTSALRAAANAKPVVVLKAGRKQDGNVAAQTHSGAIVGS
                     DEVWGAVLRRAGAVRVRSFVELFSAAKCLASRYRPVGRRLGIITNGGGPGVLAADYVN
                     ELALQLGVLSDENARTLAQLVPKAASLTDLIDLSEDATPEHYKAALDIAAKDKQLDGV
                     LVIYSPKFGGDAEAVARELVQIKPRMSKPLVACWMGDSSVQPARHALAEAQIPSFRTP
                     EAAVGAFGNIASFYQNQQLLQQTPPPLSPGAPPDVEGARLLIESVLTERRKVLTEMES
                     KALLAAFHVPVTQTLLARSSNEAMLIASQIGYPVALKIDSPDISHKSDVGGVALNVMN
                     ATAVRDIWQDMVEAVRAKQPDARINGVTVQAMARKKRGRELYIGVVTDDPFGPVIAFG
                     AGGTMIELMNDHAMELPPLNQFLARRLIERSRVHETLGAWRGAPAVDMAALEQVLLRV
                     SEMVCELPQLREMDINPIIVDEHGAIAVDARIVIGPAVQTSPGRAGKYGHLSILPYPQ
                     RYEQVWPLRGGGEYIIRPIHPDDADMLQDLMKRLTPEARYFRFVSNMAELPPGMLSRF
                     TLIDYDREMALCAVVEEESISDSGEKLTKEKIIGVSRYITNPDQASCEFSLVVDGAYG
                     GQGVGTRLMLSIMDVAREKGLREIIGLVLTQNNGMLKLMRSLGFEVKAYAEDPDFRLV
                     THPL"
BASE COUNT         1987 a         4319 c         4170 g         2071 t
ORIGIN      
        1 cgtgcagcca ttgcacgatg ttttggatga gttcctgcat gtcggtgccg ccttgaattc
       61 tcagcggaac gccccgtgcg cccgcttgcg cacgcacaac gcgggcgcgg gctgccaggc
      121 tgacgcagcg gcggcagacc tgccgtagga cggggccgcc aacagggatg agagtgctat
      181 gttattcata gctgctcgcg cttgccctac ttgcgctgga ggcataaaac atcaataact
      241 ccttcaagct gccagccacg ctggcttgcg cttttgcaga aagctctgca cgccctcctt
      301 gccctcatcg ctggcgcgga tgtcggcaat gccttcgacg gtgcgctcga tcagcaggcg
      361 cgtgatgtcg cgttcggcca cgtcttgcac cagcttcttg cattcgcgca cggcgttggg
      421 gctggcgctg gtgagggcgc tcaccagctc accgaccttg gcgtccagcg cctcggcgga
      481 gaccacctcg tgcacgaagc cgatgcgcag cgcttcggcg gcgccaaagc gctcggcggt
      541 caaaaaatag cggtgcgcgg cgcgtgcgcc catggcgcgg atgacgtagg ggctgatggt
      601 ggcgggaatc aagcccagct tgacctcgct caggcaatag ttcgccgtat cgacgctcac
      661 cgccatgtcg cacgccgcca ccagacccat gccgccggcg tacacgtcgc cctgcacgcg
      721 ggcgatggtg ggcttggggc attcgtagat ggcgcgcagc atgaaggcga gcttgcccgc
      781 gtcttcgatg ttctcgtcgc gcgtgtagtc ggccatgcgg cgcatccagt tcaggtcggc
      841 gccggcgcag aaggctgggc cttcggccat cagcacgatg gcgcgcactt ccgaagcctc
      901 gcccagctgc acgaaggcct gcgagagttc ggcgatcacc tcgtcgttga aggcgttgcg
      961 cacctcgggg cggttgagca ccacggtggc gacgtggccg gtggtggcga tgcggagggt
     1021 ttggctcatg gtcgggtttg ttcctgttgc agcatgtaga ccagatcgag ttcggggaaa
     1081 ggctggccca ggcgcgtgaa cgcggccgtg atctggccgc ggtggtgcgt gccgtggttg
     1141 aagacgtgcg tgagcgtggc ggcaaagggc agcgagacgg gcacgccgcg cgtggtggtg
     1201 tagctgagca cgccgtcaaa gccctcggac ggccagtttt cgatcagtgg ctgccagcgc
     1261 gcggcgcctt ccagcaggcg ctcggccagg agcgagcggt cgacttccac ttcctcgtcc
     1321 agcgtcacca tcggcgacac gccttcggca aagcgccgaa accacagcag atgctcgccc
     1381 accagcaggt ggttcagcgt gccatggatg cttttgaaga acaagccggt gtcgcggcgg
     1441 tagtcggtgt cgttcaccgc cgccaccgca tccagcaggc gctgcgtggc ccaggcgttg
     1501 tagcgggcca aagcagcgaa atgcgcgcgc gcgtccatgc tcaatgcccc catacccgca
     1561 agccccgatg cgcagcaaag ccgtcatagt cgctgtcgct gtgcagcagc aggtaatcgt
     1621 tttcgatgca gaacgcggcg atcagcacgt cgacggcgct gcgcaccgtc acgccgcgct
     1681 tgcgcaagta gcgatagtgc tcggcggcag cgagtgccac gtcttcaccg ccgcaaggct
     1741 caacgctcaa ggtctgcaag agctggcgcg cctggcgcaa cgggcgttcg tagcgaaagc
     1801 cgcgcagaac ttcgaagagc accagatcag gaatcaccag acgggtgttg ccgtcgtcca
     1861 gcaggcgacg caactcgacg cgcgccgccg tgtcgcgggc attgaagaaa tcgatccaga
     1921 cgctggaatc aacgaccaac atgggtcttg gtaccggtct tgcgcggctt ggcagcaggc
     1981 gcttttgcgc tcgccggatc agtggagcga ttcacgcgat acgacaccgc tggctcctgc
     2041 acggccaacg cttctgtctc gtgctgcggc tgggcctggt cttgtgcatc cagggcaacc
     2101 cagtcgtggg cgtcatcgcc ctcccatttc agcttgcctt cccacttgag aatctcgcgg
     2161 taggcggcct gccgcgccag caattgcagg ccagcctcaa cggtttcctt cttggtggaa
     2221 taagggccgg cttgcatggc cttggccagc aacgcgtcgt caatatcgat gttggttcgc
     2281 atgatgtgta taaacctcga aattggcaca cattatgcac atcacatgcg gaaaacgcca
     2341 aacttcacgt ccggcaccgg cgcgttctgc gcggcggcca gtcccagcgc cagcacgcgg
     2401 cgcgtgtcgg cggggtcaat cacgccgtcg tcccacaggc gggcggtggc gtagtaggga
     2461 tggccctgca cttcgtattg ctccttgatg ggggctttga atgcggcttc ttcttcagca
     2521 ctccagctgc cgcccttggc ctcgatgccg tcgcgcttga cggtggccag cacgccggcg
     2581 gcctgctcgc cgcccatcac gctgatgcgc gcgttgggcc acatccacaa gaagcgcggg
     2641 ctgtacgcgc ggccacacat gccgtagttg ccggcgccaa agctgccgcc gatgatgatc
     2701 gtgaacttgg gcacgttggc ggtggccacc gccgtcacca tcttggcgcc gtcgcgggcg
     2761 atgccttcgt tctcgtactt gcggccgacc atgaagccgg tgatgttctg caggaacacg
     2821 agcgggatct tgcgctggca gcacagctcg atgaagtgcg cacccttgcg cgccgactcg
     2881 gcaaacagaa tgccgttgtt ggcgacgatg ccgaccggca tgccctcgat gtgcgcaaaa
     2941 ccgcagacca gcgttgtgcc atagcgcgac ttgaattcgt gcagctcgga gttgtcgacg
     3001 atgcgggcaa tgatttcgcg cacgtcaaac ggcttgcgcg tgtcggtggg gatcaccccg
     3061 tacagctctt ctgctgcata tttgggagct gctggcgctt gcagggagcc gctgtacacc
     3121 tttttggcat tcaaacgcga gaccgcctcg cgcgccagtg ccagggcgtg catgtcgttg
     3181 tgggccaggt gatcggccac gcccgacagg cgcgtgtgca cgtcgccacc gcccagatct
     3241 tcggcactca ccacttcgcc cgtggcggct ttgaccagcg gcgggccgcc caaaaagatg
     3301 gtgccctggt tcttgacgat gatggtctcg tcgctcatgg ccggcacgta ggccccgccg
     3361 gccgtgcaac tgcccatcac cacggcgatc tgcgcgatgc cctgcgcgct catgttggcc
     3421 tggttgtaga agatccgacc gaagtggtcg cggtcgggga acacctcgtc ctgattgggc
     3481 aggttggcgc cgcccgaatc caccagatag atgcagggca ggcggttctg ctgcgcgatc
     3541 tcttgcgcgc gcaggtgttt cttcaccgtc agcgggtaat aggtgccgcc cttcaccgtg
     3601 gcgtcgttgc agacgatcat gcaatcgatg cccgacacgc ggccaatgcc ggccacgatg
     3661 cccgcgcccg gcgcgctgtc gccgccatcg cggtcggggt acatgcccaa agcggcaagc
     3721 ggcgccactt caagaaaggg cgtgcccgga tcgagcagca tttgcacgcg cacgcgcggc
     3781 agcagcttgc cgcggtcggt gtgtttcttg cgcgcggcat cgccgccgcc agcgcccact
     3841 ttttgcacct gcgcctgcag gtcatcgacc agcgcctgca tggcggcggc gttggcctgg
     3901 aagtcggcgg agcgggcgtt gagcttggtt tcgatgacgg acatgaatca cctttgctat
     3961 tttttagtga gctgcttgcg cttgatttac gtgcgctcaa gccgtttttt gttctgaaag
     4021 acccagttct tcctgcatca actcgcggat cctgaacttc tggatcttgc cggtgatggt
     4081 catcggaaag gcgtcgacaa acttcaggta acgcggcacc ttgtagtgcg cgatcttgcc
     4141 tttgcagaat tcgcgcacgc cggcttcgtc aagcgtctgg ccgggtttgg cgatgatcca
     4201 ggcgcacagc tcctcaccga acttctggtc gggcacgccg accacctgca cgtcctggat
     4261 ctgcgggtgg cggtacaaga actcttcgat ctcgcgcggg tacacgttct cgccgccgcg
     4321 aatcaccatg tccttgatgc ggccgacgat gttgacgtag ccctcgtcgt ccatggtggc
     4381 cagatcgccc gtgtgcatga agccttcggc gtcgatggct tcgcgcgtgc gtgcgtcatc
     4441 gccccaatag ccttgcatca ccgaatagcc tttggtgcac agctcgccgg tggcgccgcg
     4501 cggcacggtc tggccgctgt cggggtcgat gatcttgatc tccaggtgcg gctgcaccag
     4561 gccgacggtg gacacgcgtt tgtccagcgg tgtgtcggtg ctgctctggc aactgacggg
     4621 cgaggtctcg gtcatgccgt aggcaatcgt cacctcggcc atgttcatgt cggcctgcac
     4681 gcgcttcatc acctcgaccg ggcacgggct gccggccatg atgccggtgc gcagcgtgct
     4741 gaggtcgtat ttcgcgaagt cgggatgatc gagctcggca atgaacatgg tgggcacgcc
     4801 gtgcaggccc gtgcattttt cagcctgcac cgtctgcagc accgtcagcg ggtcgaacgc
     4861 gtcgttgggg tagacgatgc agccgccgtg cgtcagcacc gccaggttgc ccagcaccat
     4921 gccgaagcag tggtacagcg gcacggggat gcacaggcga tcctcgtgcg tcagcttcat
     4981 cgcctcgccg atgaaaaaac cgttgttcag gatgttgcgg tgcgtcagcg tggcgccctt
     5041 ggggaagccc gtggtgccgc tggtgaactg cacgttgatc gggtcgcgcg ggtgcagcag
     5101 cctggcgatg cgcgccacgc gctcgtcggc cgggtcgccg cgctggatca ggtcggaaaa
     5161 gcgcatgaaa cccggttcat ccgcaccttc tccggccacg tcaatccaga tcgcgtgctt
     5221 caggtgcggc agcgtctgcg attcgagcgg ctcgccggcc ttggcgtgcg cccactcggg
     5281 cgccagctcg cgcagcatgc cgatgtagtc gctggtcttg aagcgcggca tggtcaccag
     5341 cgccttgcaa tcgaccttgt tcaaggcgta ttcgacttcg gccgtgcgat aggccgggtt
     5401 gatgttgacc aacaccagcc ccgccttggc ggtggccagc tgcatcagca cccattcggc
     5461 attgttgtgc gaccagatgc cgacgcggtc gccaggcgac aggcccaggc ccagcaaggc
     5521 gctcgccagg cggttggcct cggtctgcaa ctggcggtag gtgtagcggc gctgttggtg
     5581 cgccaccacc agcgcctcgc gctcggggta acgctcgacc atggcgtcga acaaggcgcc
     5641 aatggtttgc tcgatcagcg gcggctcggt ggcaccgcgg gcgtggctgg gcgcggcggt
     5701 gggggaagca ctcacgatgg cggtctccgg taggcgctct gcggcgcatg acaagccaat
     5761 agcttagggc atcgcgcggc gcgatgcttg ccagcggggt tgcaaatcgt gccatgcctg
     5821 cgacacactg tgctggccat gccacgcgat gccaccacca ctggtcagac cccgatcgcc
     5881 ttcgtgcgcg ccatcaccgg ggcctacgcg gcgcgcgggc tggacccgtc ggccgcgctg
     5941 caaaaggcac gaatcgcgcc agccgatctg gcgcggtcgc gggcgcgcgt caccgccgtg
     6001 cagttcgagc gcctgtgcgc cgccgccatg caggagctgg acgacgaggc ccccggctgg
     6061 ttcagccgcc gcctgccctg gggcagctac ggcatgctgg cgcgcgccag catcagcgcg
     6121 ccgacgctgg gcgtggcgct ggcgcgctgg tgccgccacc acgggctgct gacggacgac
     6181 gtcacgctca ccctcggcca ggccggcggc caggcgcggg tcgagatcgc cgagcaccgc
     6241 gatctgggtg cgctgcgcga gttcgcgctg ctctcgctgc tgcgcaacct gcacgggctg
     6301 gcctgctggc tggtggactc gcacatcgcg ctgcagggcg cgcagttccc gctgccagcg
     6361 ccgccgcatg ccgacatgta cacccgcctg ttccccggcc cggtgcagtt cggcgcaccg
     6421 cgcgcgggcc tacagttcga cgcccactac ctgcaactgc cgctgcgccg cgacggcgcg
     6481 gcgcttgacc agatgctgcg ccgcgcgctg cccatcctgg tgtggccgta tcggcgcgac
     6541 cgcctgctgt cgcgccgcgt gcgccgcctg ctgcagcagc cccatgccgc ccacaccgcc
     6601 gaaacgctgg ccacgcggct gggcctgtcg gcgcgcagtc tgcaccgcca gttgcaaggc
     6661 gaaggcacca gcctgcaggc gttgaaggac gaggtgcggc gcgagcgcgc cagcgacctg
     6721 ctgctgcgca cgcagtggcc catcgcgcgc gtggcgcgcg ccgccggctt taccagcgac
     6781 aagagctttg cccgcgcctt ccggcaatgg acgggccaca ccccgcagac tcaccgccag
     6841 cgcggcagtc cgctggagtc ataaatcaaa tcaaccgcca gcgcttttct gacaagcgcg
     6901 agcagctatt atttaactag cattcgacag atcgacgcca cacaacacca ggagacccca
     6961 tgccccagcc caccatcccc gacgccctgt ggcgcctgcc cgccagccag accgccgcgc
     7021 tggtgcgcgc ccaccaggtc agcgccaccg aggtcgcgcg tgctgcgctg gcccgcgtgg
     7081 acgcggtcaa cccgcgcctg aacgccatcg tcgaatgccg gcccgacgag gtgctggcgc
     7141 gcgccgccgc catcgatgcc gcgctggcgc gcggcgaaga ccccgggccg ctggcaggcg
     7201 tgcccatcac cacaaaggtc aacgtcgacc aggccggcta cgccaccacc aacggcctga
     7261 aatcgcaaaa ggacttgatc gccaccgagg acgcccccat ggtgcgcggc ctgatggcgg
     7321 acggcgccgt gctgctgggg cgcaccaacg tgcccgcgtt ctgctaccgc tggttcacca
     7381 gcaaccagct gcacggccgc acgctcaacc cgcgcaacgc ggcgctgaca cccggcggct
     7441 cgtccggcgg cgcggccagc gcggtggcgg ccgggttggg cgccatcgcg cacggcaccg
     7501 acatcgccgg ctccgtccgc tacccggcct acgcctgcgg cgtgcacggc ctgcggccga
     7561 gcctggggcg ggtggccaac ttcaacgcca cggcgcccaa agaccgcagc atcggcgggc
     7621 agatcatggc cgtgtccggt ccgctggcgc gcaccgtgga cgacctgcgc ctgggcctgg
     7681 cggccatggc gcggcccgac acgcgcgacc cctggtacgc gcccgtgccg ctgtcgggcc
     7741 ccgacctgcc gcgccgcgcc gctgtctgtc tgcgccccga cggcatggcc accgcacccg
     7801 agatctgcgc cgccctgctg gacagcgccg accgcctgcg tgatgccggc tgggtggtgg
     7861 acgaggtgga cgccctgccg ccgctgcagg aggccgtgcc cctgctcatc accctgtgga
     7921 tgggcgacgg ctacgacgcc atggtgcaag ccgccgaggc cgaaggcgat cccggcgcca
     7981 tcgccgcgtt ggccggccag gccgagttcg cgcgcggcat cggcttgccg cagtacagcg
     8041 acgcgctggt gcgccgcgcc acgctggcac gcgcctggca acgctttctg gccgagcgct
     8101 acagcgccgt gctgctgccg ctcagcgccg agctgccgtt cgaggacgac ctggacctga
     8161 aggacgccgc cagctaccag cgcgtgtggt ccgcgcagat gagccagatc gccctgcccc
     8221 tgaccggcct gcccgcgctg gcgctgacca ccggcaacgt gggcaacggg gtacccgtcg
     8281 gcatccagat cgtggccgcg cgctttcgcg aggatgtgtg cctgggcgtg gccgaggcga
     8341 tcgaggcgcg cctgcccgcg ctggcacccg ccctgccctg acccgccagc cgtggccgcc
     8401 gacctgcgtg tgcgaggcac ccaagtcagc gcggcaacgg gttgaccacc cgcgcatgcc
     8461 agcccacgaa cagcggcgcg ccgacgatct ccaatacggt aaaggcaatg aacggcgccg
     8521 gtggccagcc gacgaccagc atcgacaaca ggcggcccag gccgccgatg aagatggcgc
     8581 cccagacgac gcaaaacagc accgtctgcg cgtgcaggcg cggcaccatc cagaacgcgg
     8641 ccaaccccag acccagccag acgccgccca gaaagcgcag gttgctggcc aacagcggat
     8701 tccccggcag gtccagagcg gcgtacagcg ggtcatgcac gcccatcatg gtgatggccc
     8761 cggtcaggat gggaatcaag gccagcacgc cggtcagccc ctggagaagt cggtacgaag
     8821 tcatggcggt ccagaaagaa gcccacggat ggcggccatc gggcccgcgc gggtacgcca
     8881 gcctgcgccc gcaggtcgct ggctgccagc atatccgggc ctccaggcga taccactacc
     8941 ccaggcgaat gggtacagtt gcgcgtatgc atacccaggc gcgccccctc ttcaccgcgg
     9001 aacacgacgc cttccgcgaa caggtgcgcc gcttctgcgc gcaggagatc gccccccacc
     9061 acgccgcctg ggaagacgcc cacggcgtgc cgcgcgcgct gtggcagcat gcgggcgcca
     9121 acggcctgct gtgctgctgg ctgcccgagg cgctgggtgg cccgggcgcg gacctgctgt
     9181 tcgacttcat cgtcgccgag gaactgggcc gcatcggcgc caccggcccg ggctttccgc
     9241 tgcattcggt catcgtcgcg ccctacctgg cggcgcatgg cacgcccgcg ctgcaacagg
     9301 cgctgctgcc ggccatggtg gcgggcgaga agatcgccgc catcgccatg accgagcccg
     9361 gcaccggcag cgacgtggcc gccatccgca cgcaggcgcg gcgcgacggc gagcactacg
     9421 tgctgagcgg ccagaagacc ttcatcacca acggccacaa cgccgacgtg gtggtggtgg
     9481 cctgcaagac cgagccggac cagggcgccc gcggcgtatc gctgctcgtc gtcgagcgcg
     9541 gcatgcccgg cttcacgcgc gggcgcaacc tgcgcaagat cggccagcac gcgcaggaca
     9601 cggcagagct gttcttcgaa gaggtgcgcg tgccggccag tcacctgctg ggcgaagaag
     9661 gccagggctt caaatacctg atgcagaagc tggcgcaaga gcgcctgctg gtcagcgtcg
     9721 gctgccaggc gcgcgccgag gcggcgctgc agtggacggt ggactacgtg aaggagcgca
     9781 aggcgttccg ccagcgcgtg gccgactttc agaacacgcg cttcaagctg gccgccgtcg
     9841 ccaccgacat caccgccggc cgcgcgtatt gcgacacgct catcgcccag cacctggcgg
     9901 gcgaactcga cgccgtgggc gccgccgccg gcaaactgtg gcacagcgaa ctgctcggcc
     9961 gcgtgaccga cgagtgcctg cagctgtttg gcggctacgg ctacatggct gactacccga
    10021 ttgcgcgcgc gtacgccgac gcccgcatcg agcgcatcta cgccggcacg tcggagatca
    10081 tgaaggagat cgtggcgcgc gatctgctgg atgcgcccag gtgatgagcg cggcaggttt
    10141 gtcgccaaac ccgcaaacaa tccaagcccg ttcgcgtgag agacccaggc ttcgacaagc
    10201 tcaacccgaa cggatgatcg gccgtcggac tgttgacggg ctcatccatg cgcaaaaaaa
    10261 gctgccagcg cttgccagac aagcgccagt agctcacatt ttgatagtta tcacaatggg
    10321 tgcgtgacca gccggaagtc cgggtcttcc gcgtacgcct tcacctcaaa ccccaggctg
    10381 cgcatcagct tcagcatgcc gttgttctgc gtcagcacca ggccgatgat ctctcgcagg
    10441 cctttttcgc gcgccacgtc catgatgctc agcatcagcc gcgtgcccac gccctggccg
    10501 ccgtaggcgc catccaccac cagcgaaaat tcgcagctcg cctggtcggg gttggtgatg
    10561 tagcgcgaga cgccgatgat cttttccttg gtcagcttct cgccgctgtc gctgatgctt
    10621 tcttcctcga ccaccgcgca cagcgccatc tcgcggtcgt agtcgatcaa ggtgaagcgc
    10681 gacagcatgc cgggcggcag ctcggccatg ttggagacaa agcggaagta gcgcgcctcg
    10741 ggtgtcaggc gcttcatcaa gtcctgcagc atgtcggcgt cgtccgggtg aatcggccgg
    10801 atgatgtatt caccgccgcc gcgcagcggc cacacctgct cgtagcgctg cgggtagggc
    10861 aggatcgaca ggtgcccgta cttgcccgcg cgccccggcg aggtctgcac cgccgggccg
    10921 atgacgatgc gcgcatcgac ggcaatggcg ccgtgctcgt cgacgatgat ggggttgatg
    10981 tccatctcgc gcagctgcgg cagctcgcac accatctcgg acacgcgcag cagcacctgc
    11041 tccagcgccg ccatgtcgac ggccggcgcg ccgcgccagg cgcccagcgt ctcgtgcacg
    11101 cgcgagcgtt cgatgagtcg gcgcgccagg aactggttga gcggcggcag ctccatggcg
    11161 tggtcgttca tcagctcgat catggtgccg ccggcgccga aggcgatgac ggggccgaac
    11221 gggtcgtccg tcaccacgcc gatgtacagc tcgcgcccgc gcttcttgcg cgccatggcc
    11281 tgcacggtga cgccgttgat gcgcgcatcg ggctgcttgg cgcgcaccgc ttccaccatg
    11341 tcctgccaga tgtcgcgcac ggcggtggcg ttcatcacgt tcagcgccac gccgccgaca
    11401 tcgctcttgt ggctgatgtc gggcgagtcg atcttcagcg ccacggggta gccgatctgg
    11461 ctggcgatca gcatcgcctc gttgctgctg cgcgccagca gcgtctgcgt gaccggcacg
    11521 tgaaacgccg ccagcagcgc cttcgattcc atctcggtca gcaccttgcg gcgctcggtc
    11581 agcacgcttt cgatcagcag gcgcgcgcct tccacgtcgg gcggtgcgcc gggcgacagc
    11641 ggcggcggcg tctgctgcaa gagctgctgg ttctgataaa agctggcgat gttgccaaag
    11701 gcgccgacgg ccgcctcggg cgtgcgaaag ctcggaatct gcgcctcggc cagggcgtgg
    11761 cgcgccggct gcaccgacga atcacccatc cagcaagcca ccagcggttt gctcatgcgc
    11821 ggcttgatct gcaccagctc gcgcgccacg gcctcggcat cgccgccgaa cttgggcgaa
    11881 tagatgacca gcacgccatc gagctgcttg tccttggcgg cgatgtcgag cgcggccttg
    11941 tagtgctctg gcgtggcgtc ttcggacagg tcgatcagat cggtcagcga cgcggccttg
    12001 ggcaccagct gcgccagcgt gcgggcgttc tcgtcggaca gcacgcccag ttgcagcgcc
    12061 agctcgttca cgtaatcggc cgccagcacg ccggggccgc cgccgttggt gatgatgccc
    12121 aggcgccggc cgaccgggcg gtagcgcgag gccaggcact tggcggccga gaacagctcg
    12181 acgaaggagc gcacgcgcac cgcgccggcg cggcgcagca cggcgcccca cacctcgtcg
    12241 ctgccgacga tggcgcccga gtgcgtctgc gcggccacgt tgccgtcctg cttgcggccg
    12301 gccttcagca ccactaccgg cttggcgttg gccgccgcgc gcagcgcgct ggtaaagcgc
    12361 cgcgcgttgg caatgccttc catgtagacc acgatgccct gcgtgcgccg gtcgttggcc
    12421 agaaaatcca gcacgtcggc cagcccgacc tgcgtgtgcg cgcccagcgc caccacggcc
    12481 gaaaagccga cgtggttctg ccgcgcccag tcgaggatcg acgccgtgag cgcgcccgac
    12541 tgcgaca
//
LOCUS       DAOMVO010000037        12492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1149_length_12492_cov_13.546273, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000037 DAOMVO010000000
VERSION     DAOMVO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12492
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1149_length_12492_cov_13.546273"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKV30_03370"
     CDS             complement(<1..755)
                     /locus_tag="PKV30_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRL29294.1"
                     /translation="MSGAIHLDRHGEHVALIRIDRPEARNALTPRMLCALADAFLAVR
                     DDAEVRAVVLTGTGDVAFCAGGDLGLTLPLMTGARAPQDEFDRRLLDDPRTLEASSLR
                     GFDIVKPTICAINGTCLAAGFELMLGTDIRIAAEQSQFGLPEVQRALLPFAGSMARLP
                     RQVPAALAMQLLLTGRPISAELALQWGLVTELWPSDRLLPRALELAEQIAGNGPVAVQ
                     AVRRAAIQGSGLPLPDAFALEDAAKREVMRSQDA"
     gene            complement(752..1570)
                     /locus_tag="PKV30_03375"
     CDS             complement(752..1570)
                     /locus_tag="PKV30_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29295.1"
                     /translation="MRNILGMTECGGIIAIEPCAMPRTPGSVGLPIPFVTVRAVDAAG
                     EPVPAGADGELQIRGPNVSPGYTDAAKNEGVFLPGGWLASGDVGHVYAQGRLYVTGRA
                     KDMIIRSSHNIDPLIIEDTLQKHPDVLMAAAVGAPDEYAGELPVAYVTLRPGATTDAP
                     SLLLFAEQQMPERPAVPKQIVVLDALPVTAIGKFFKPRLREMAAQQVIEDRLAQRGLS
                     GTVAAQVLADSKGVQVIFDCGGNAAAAATVKDMMAPFALRWRIADHASAPQVGA"
     gene            1560..2705
                     /locus_tag="PKV30_03380"
     CDS             1560..2705
                     /locus_tag="PKV30_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29296.1"
                     /translation="MLRTGMPKRRSNASARSGGQGRAAGQQRPHARDVDAIGRHRQQR
                     RHHRRHRGQQRGFFALGKAPVALHEIGVAHAQQRGQHDRATDQKHRQAVHERTRDVKQ
                     RKAVDDHVALGRAVHAGRAGRRTQLIAVLVLRQLGAAGGAAGVKKRGHVVHRGAGRQR
                     RIALRGHGLIEIQRALGWRRRPDRQHVRQLRAAPPNARHARPDVQLGPRPQGHQHVHA
                     GGLQQVGDVLFAQQVVDGAGDAHGFSPPQREVRLRNRRQQKGHARRLIDAERLKQARG
                     APDLGQQLRIGPLLGRLVKVRALDVLERDALRVLGRLRFQRVHHAGVRQVGLERVRLD
                     RANVVGGMNGAYAHGRSWANPTSSHRHHGGSPSVGQEKCDPRGAFRL"
     gene            complement(2730..3149)
                     /locus_tag="PKV30_03385"
     CDS             complement(2730..3149)
                     /locus_tag="PKV30_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HRL29297.1"
                     /translation="MQTAPPIRTLPRAEPARQPELAAFSARLTGQSAVPTSDSAAQGE
                     LVAVLNRNTGLLQWKLSFSGLSGPVRGANFHSPGMAGEVAQPVLSLGRSVLSPSEGRA
                     MLSARQRADLLAGQWYINLRTARYPDGEIRGQLIEKH"
     gene            complement(3361..4002)
                     /locus_tag="PKV30_03390"
     CDS             complement(3361..4002)
                     /locus_tag="PKV30_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HRL29298.1"
                     /translation="MRPNPLMPSRHSAAVLAAALLASACASVDLGPRYDPPPVRVPQP
                     LPPPQAPLPPIAQPQAVPPSMPVPQPLPPVGQTVPVAPPPPVTPVDPSAHLVALTTRL
                     DGGSVMPPSRSAATAQLDALYDSNTRVLRWKTSWSGLSGPITGVQFHGPAEYGQNAPA
                     VMTWPGPFGPTYEGRATLNANQAIDLMDGRWYVSVYTTAYPSGELRGQLRVVN"
     gene            complement(4234..4695)
                     /locus_tag="PKV30_03395"
     CDS             complement(4234..4695)
                     /locus_tag="PKV30_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL29299.1"
                     /translation="MDLILWRHAEAQEPEGDMTDLDRTLTRRGDKQAARMAAWLDRQL
                     PEGTRILASPAVRTEQTVRALGRKYRLCDELRPGASPEQLLQLVGWPLAKAPVLVVGH
                     QPTLGETISRVLGLAEPECSVKKGAVWWLRSRERESGLQTVVVTVQAAELL"
     gene            complement(4719..6776)
                     /gene="ppk1"
                     /locus_tag="PKV30_03400"
     CDS             complement(4719..6776)
                     /gene="ppk1"
                     /locus_tag="PKV30_03400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRL29300.1"
                     /translation="MPTDAADTQAILARYPLQDRDASILDFNLRVLHWAERPDVPLLE
                     RLRYLCIVSSNLDEFFEVRAEPHLTAWRAGTDVQGGMRHLMAAAHALVERQYAIYNEV
                     LLPAMATRRMRIVSHIDRTEAQRKWVREYFLREVKPLLVPVALDPAHPFPLVANKSLN
                     FIVQLKGKDAFGRSNEIAIVKVPRVLPRVLRLPERVTGGETLFVMLSSVIRAHLADLF
                     PGRDVGDFSHFRVTRDSDLSVDEEEVKNLRTALRQELHQRHYGEAVRVEVSSACSQAL
                     AEFLLRQFKLPPEALFKVQGPVNLVRLQQLISLVDEPALLFAPYRASYPMQLQRGESV
                     FERLRRGDVLIHQPFESFEGVIDFLHEAVHDPLVLAIKQTIYRAGNDSRMMDLLIDAQ
                     RRGKEVTAVVELKARFDEEANINWAERLEQAGAQVVYGVVGLKTHAKMLLVTRREGRQ
                     LRRYGHLSTGNYNPRTAALYTDLSMLTADARLTSDMDQLFVHLASHSRLPRMQRMLLA
                     PFTLHKQMLARIDAVAQAAKKGRGGRIVAKMNALTDEALIVALLRAGDAGAKIDLIVR
                     GACMLPAQLPPNIRVRSIIGRFLEHSRVFYFRAGADEQVWLSSADWMNRNMLRRVETA
                     WQVTDPALRQRIIDECLLAYLHDARDAWVMGEGGRYRSVVPPGAEPGPGAQDALMARY
                     RAA"
     gene            complement(6829..7884)
                     /locus_tag="PKV30_03405"
     CDS             complement(6829..7884)
                     /locus_tag="PKV30_03405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRL29301.1"
                     /translation="MKIALVTDAWQPQVNGVVTTLVELVNSLRAGGDEVIVIEPGQFK
                     TRPCPGYPGIDLAVRPGARVRELLDSAQPDAVHIATEGPLGWAARRHCLRRGWAFTTA
                     FHTKFPEILQAAVKLPLGWGYAFFRHFHRPSSGVMVPTRGVAAGLAERGFKNLREWTH
                     GVDTRWFGFSPKAADYAPLGRFARPLHLFVGRVSYEKNIQAFLELDLPGTQVVCGVGP
                     VEDKLRARFPQVRWLGVLPRDELARVYAAADVFVFPSRADTFGLVMLEAMATGTPVAA
                     YPVDGPTEVLAAAPGHAVGGVMHQDLRHAVQQALRIPREAARAHAQRFGWDEAARLFR
                     GYLVPARAEGVPQAVPG"
     gene            complement(8081..9046)
                     /locus_tag="PKV30_03410"
     CDS             complement(8081..9046)
                     /locus_tag="PKV30_03410"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRL29302.1"
                     /translation="MAPVAMNLDAALGHAAQHLLRGDLAPLDRPVPPAMGTADRDAPW
                     PLSPGGGGDDADEPSMARDRPRFRAIFISDIHLGTPGCQAGPLLDFLKEHESETLYLI
                     GDIIDGWQLRRRWYWPQSHNDVVQKLLRRARKGCHVVFVPGNHDEFARGFLNHNFGGI
                     DVVEDSVHVTVDGRRLWITHGDYFDGVIQHAKWLAYVGDHAYEMVLKLNRHFNSLRAR
                     LGLPYWSLSAYLKHKVKKAVNYVSDFEGAVAAEARRRGYDGVVCGHIHHAEIRDVDGT
                     LYCNDGDWVESRSALVEHHDGRLELVFWQAGHGAEVMSQRAVETV"
     gene            complement(9052..9828)
                     /locus_tag="PKV30_03415"
     CDS             complement(9052..9828)
                     /locus_tag="PKV30_03415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRL29303.1"
                     /translation="MPGGAFVRDPVGATGLDGGIQVSWARQLDEIRAAQRLRYQVFVH
                     EMGARLATTLPGHDIDLFDDYCEHLLVRDEATQTVIGTYRVLTPVQAKRVGSTYSDTE
                     FDLTRLRSLRHRMVELGRSCVHPDHRHGGVIMALWTALGEFMVRNNLDTMIGCASIPM
                     MQGGVFNGDVAASIWRQVREQYMAPIEFQVRPRLALPLENLNDRLPVEPPALIKGYLR
                     LGTKVLGAPAWDPDFNSADLPMMMRFADLHPRYRKHFLGA"
     gene            10097..10453
                     /locus_tag="PKV30_03420"
     CDS             10097..10453
                     /locus_tag="PKV30_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL29304.1"
                     /translation="MEQDQAIKVFESLASGLRLDVYRLLMRAGPEGLVAGDIASALNV
                     PPTNLSFHLKALTQSGLLGVTPEGRYQRYRANLALMQAMVDYLTAECCAGHPEQCADL
                     RGERVCAPASATTESI"
     gene            10552..11070
                     /locus_tag="PKV30_03425"
     CDS             10552..11070
                     /locus_tag="PKV30_03425"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRL29305.1"
                     /translation="MTIDRPTLNVLFVCTGNSARSILAESILNHPAIGQRRFRAFSAG
                     SHPAGHVQPLALALLAQYHYPTDGLRSKNWDEFSGPSAPRLDFVFTVCDRAAGEACPY
                     WPGQPMTAHWGVEDPAAVQGSAEEQRRAYANAFMVLKNRIAMFAALPFDKLDRLSLKQ
                     QLDGIGAAAPVA"
     gene            11150..12190
                     /gene="arsB"
                     /locus_tag="PKV30_03430"
     CDS             11150..12190
                     /gene="arsB"
                     /locus_tag="PKV30_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRL29306.1"
                     /translation="MGLFERYLTLWVALAMAAGVALGTVMPGMFQTIGRMELARVNLP
                     VGLLIWVMIIPMLVKVDFGSLGQVRRHARGIGVTLFVNWLVKPFSMAFLGWLFIRHVF
                     APYLPADQLDSYIAGLILLAAAPCTAMVFVWSRLTNGDANFTLSQVALNDGIMVVAFA
                     PLVAFLLGVSAITVPWATLLVSVLLYIIVPLVIAQLWRRALLSRGQAAFDAAMASTGP
                     WSIGALLLTLVLLFAFQGMQIVRQPLIIAMLAVPILIQVLFNSALAYGLNRALGESHH
                     VACPSALIGASNFFELAVAAAIALFGFESGAALATVVGVLIEVPVMLLVVRVVNASKG
                     WYERGAAGSRSA"
     gene            complement(12198..>12492)
                     /locus_tag="PKV30_03435"
     CDS             complement(12198..>12492)
                     /locus_tag="PKV30_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29307.1"
                     /translation="LPAAPDSSRLIGIEVLELGPVVKLLSSAALVDGGTEVVFRAVRY
                     PIGTADEPHSFIVHWRHGGPGVVKGVASLPQDMRAALQAGLVAANGAASVPDR"
BASE COUNT         1984 a         4271 c         4227 g         2010 t
ORIGIN      
        1 gcgtcctggc tgcgcatgac ttcgcgcttg gcggcgtctt ccagcgcaaa cgcgtcgggc
       61 agcggcagcc cgctgccctg gatggcggcg cggcgcacgg cctgcaccgc caccgggccg
      121 ttgccggcga tctgctcggc cagctcgagc gcccggggca acaggcggtc gctcggccac
      181 agctcggtga ccagacccca ttgaagcgcc agttcagcgc tgatcggccg gcccgtgagc
      241 agcaactgca tggccagcgc ggccggcacc tgccgcggca gccgcgccat cgagccggca
      301 aagggcagca aggcgcgctg cacctccggc aggccgaact gcgactgttc ggccgcgatg
      361 cggatgtcgg tgcccagcat caactcgaat ccagccgcca ggcaggtgcc gttgatggcg
      421 cagatcgtgg gtttgacgat gtcgaagccg cgcagcgacg acgcctccag cgtgcgcggg
      481 tcgtccagca ggcgccgatc aaactcgtcc tgcggcgcgc gcgccccggt catgagcggc
      541 aaggtcaggc ccagatcgcc gccggcgcag aaggcgacat cgccggtgcc ggtcagcacc
      601 acggcgcgca cctccgcatc gtcacgcacc gccagaaagg catcggccag cgcgcacagc
      661 atgcggggcg tcaaggcgtt gcgcgcctcg ggccggtcga tgcggatcag cgcgacatgc
      721 tcgccgtgcc ggtcgaggtg gatggcgccg ctcatgcacc cacctgcggc gcgctggcgt
      781 ggtcggcaat gcgccagcgc agcgcaaagg gcgccatcat gtccttcacc gtggcggccg
      841 ctgccgcatt gccgccgcag tcgaagatca cctgaacgcc cttgctgtcg gccagcacct
      901 gcgccgccac cgtgccggac agcccgcgct gcgccagccg gtcttcaatg acctgctggg
      961 ccgccatctc gcgcagccgg ggcttgaaga atttgccgat ggcggtcacc ggcaatgcat
     1021 cgagcacgac gatttgcttg ggcaccgcag gccgttcggg catctgttgc tctgcaaaca
     1081 agagcaggct tggcgcgtcg gtggtggcgc cgggccgcaa ggtgacgtag gccaccggca
     1141 gttcacccgc gtattcgtcc ggcgcaccca cggcggcggc catcagcacg tcggggtgtt
     1201 tctgcagcgt gtcctcgatg atcagcggat cgatgttgtg gctgctgcgg atgatcatgt
     1261 ccttggcgcg cccggtcacg tacaaacgcc cctgcgcata gacgtgaccc acgtcgccgc
     1321 tggccagcca gccgccgggc aggaagacgc cctcgttctt ggccgcgtcg gtgtagccgg
     1381 ggctgacgtt gggcccgcgg atctgcagtt cgccatcggc gcccgcgggc accggctcgc
     1441 ccgccgcatc cacggcgcgc accgtgacga acggaatcgg caagccgacc gagcccggcg
     1501 tgcgcggcat cgcacagggt tcgatggcaa tgatgccgcc gcactcggtc atgccgagga
     1561 tgttgcgcac cggaatgccg aagcgacgct cgaacgcttc cgccagatcc ggcgggcagg
     1621 ggcgagccgc cggtcagcag cgcccgcacg cgcgagatgt cgacgccatc ggccggcacc
     1681 gtcagcagag aagacatcac cgtcggcacc gcggccagca acgtggcttt ttcgcgctcg
     1741 gcaaagcgcc agtagcgctg cacgaaatcg gtgttgcgca tgcccagcag cgtgggcagc
     1801 acgaccgtgc caccgaccag aaacatcgac aagccgtaca cgaaagaacc cgcgacgtga
     1861 aacagcggaa agccgttgac gatcacgtcg cgctcggacg cgccgtacat gcaggccgcg
     1921 ccggccgccg cacacagctg attgcggtgt tggtgctgcg ccagcttggg gctgccggtg
     1981 gtgccgccgg tgtgaaaaag cgcggccacg tcgtccatcg cggcgcgggc aggcagcggc
     2041 gcatcgcttt gcgcggccac ggcctgatcg aaatccagcg cgccctcggt tggcgccgtc
     2101 gccccgaccg ccagcacgtg cgtcagttgc gggcagcgcc gccgaatgcc cgccacgcgc
     2161 gaccagatgt ccagctcggg ccgcggcccc aaggccacca gcacgttcac gccggcggct
     2221 tgcagcaggt cggcgatgtg ctcttcgctc agcaggtagt tgatggggca ggcgatgccc
     2281 acggcttcag ccccccacag cgtgaagtgc gcctgcggaa tcgccggcag cagaaaggcc
     2341 acgcgcggcg cctgatcgac gctgagcgat tgaaacaggc gcgcggtgcg ccggacctgg
     2401 gccagcaact gcggataggt ccactgctgg gccggctcgt caaggtccgc gctttggatg
     2461 tacttgagcg cgatgccctc agggtgcttg gccgcctgcg tttccagcgc gtgcatcacg
     2521 ctggtgtaag gcaggtaggc ctcgaacggg tgcgcctcga tcgcgcgaac gtcgtcgggg
     2581 gaatgaatgg ggcgtatgct catgggcgct cctgggccaa tccaacgtca agccaccgcc
     2641 atcatggcgg atcgccgagc gtcgggcagg aaaaatgtga tccgcgcggc gctttccgtc
     2701 tttgaccccc gcgcgcctcg ccgccgtgat cagtgcttct cgatcagctg cccgcgaatt
     2761 tcgccgtcgg gatagcgcgc ggtgcgcagg ttgatgtacc actgcccggc cagcagatcg
     2821 gcgcgctggc gggcgctcag catggcgcgg ccttcggagg ggctgagcac ggagcggccg
     2881 agcgacagca ccggctgcgc cacttcgccc gccatgccgg ggctgtgaaa gttggcgccg
     2941 cgcaccgggc cgctcaggcc gctgaagcta agcttccact gcagcaggcc ggtgttgcgg
     3001 ttgagcacgg ccaccagctc gccctgcgcc gcgctgtcgc tcgtcggcac ggcgctctgg
     3061 cctgtgaggc gcgcgctgaa agcggccagt tcgggctggc gcgccggctc ggcacgcggc
     3121 agcgtgcgga taggcggtgc ggtctgcacc ggcggcggac tggcgcacgc ggccagcaac
     3181 ccgacaacgg caaggcacag gcccagccgc gtcagttcag ctgggcggca aaaaagtgat
     3241 gcaagagggg aggagcgcgc gcgaaacatg ccgggcacca tatcaccgcc cggcgcgccc
     3301 ggcgccgcgt ttacagccgg ataccaagct gacgaccggc cagaggcgac ggccggccgt
     3361 tcagttcacc acgcgcaact ggccgcgcaa ctcgcccgac ggataggccg tggtgtagac
     3421 gctgacgtac cagcgtccat ccatcaggtc gatggcctgg ttggcgttga gcgtggcacg
     3481 gccttcgtag gtggggccga aggggccggg ccacgtcatg acagccggcg cgttctgccc
     3541 gtattcggcc gggccgtgga attgcacgcc ggtgatgggc cccgacaggc ccgaccaact
     3601 ggtcttccag cgcagcacgc gggtgttgct gtcgtacagc gcgtcgagct gggccgtggc
     3661 ggcgctgcgc gagggcggca tgacgctgcc gccgtccagg cgcgtcgtca gcgccaccag
     3721 gtgcgcgctc ggatcgaccg gcgtcaccgg cggtggcggc gcgaccggca ccgtctggcc
     3781 caccggcggc agcggctgcg gcaccggcat cgatggcggc acggcctgcg gttgggcgat
     3841 gggtggcagc ggcgcctggg gcggcggcag cggttgcggc acgcgcactg ggggcgggtc
     3901 gtagcgcggc cccaggtcga ccgacgcgca ggcgctggcc agcagtgcgg cggccagcac
     3961 ggcggccgaa tggcgggatg gcatcagggg gttcgggcgc atgcggacaa ctcctgctca
     4021 tgggtcttgg aaaatgggtg cgatattaag cgccgcagac gtcgccgcgc tgtaggccgt
     4081 cgccgaccgg cgcggcgcac gccccacgaa cgcggggtgc gcgccctatc aaccctcggt
     4141 gacacgaaga atactatcaa taagtgagct gctcgcgctt gtccatcaag cgccagaggc
     4201 cgatttgatc gatttttctt gatcggtgcc gactcaaagc agctccgccg cctgcaccgt
     4261 caccaccacg gtttgcagcc cgctctcgcg ctcgcggctg cgcagccacc agaccgcgcc
     4321 ttttttcacc gagcactccg gctcggccag gcccagcacg cgcgagatgg tttcacccag
     4381 cgttggctgg tggccgacca ccagcaccgg cgccttggcc aacggccagc cgaccagttg
     4441 cagcagttgc tcgggcgatg cgcccgggcg cagttcgtcg cacaggcggt acttgcggcc
     4501 gagcgcgcgc acggtctgct cggtgcgcac ggccgggctg gccaggatgc gcgtgccttc
     4561 aggcaactga cggtccagcc acgccgccat gcgcgcggcc tgcttgtcgc cgcgccgggt
     4621 cagcgtgcgg tccaggtcgg tcatgtcacc ttcgggttct tgcgcctcgg cgtggcgcca
     4681 cagaatcagg tccatggttc ggctcgttga agttgcaatc aggcggcccg gtagcgcgcc
     4741 atcagcgcgt cttgcgcgcc ggggcccggc tcggcgccgg gcggcaccac gctgcggtag
     4801 cggccgccct cgcccatcac ccaggcgtcg cgcgcgtcgt gcaggtaggc cagcaggcat
     4861 tcgtcgatga tgcgctggcg cagcgccggg tcggtgactt gccaggcggt ttcgacgcgg
     4921 cgcagcatgt tgcggttcat ccagtcggcg gacgacagcc acacctgctc gtcggcaccg
     4981 gcgcgaaagt agaacacccg cgaatgctcc agaaagcgcc cgatgatgct gcgcacgcgg
     5041 atgtttggcg ggagctgcgc cggcagcatg caggcgccgc gcacgatcaa atcgatcttg
     5101 gcaccggcat cgcccgcgcg cagcagcgcc acgatcagcg cctcgtcggt cagggcgttc
     5161 atcttggcga cgatgcgccc gccccggccc ttcttcgccg cctgcgccac ggcgtcgatg
     5221 cgcgccagca tctgcttgtg cagcgtgaag ggcgccagca gcatgcgctg catgcgcggc
     5281 aggcgactgt ggctggccag gtgcacgaag agctggtcca tgtcggacgt gaggcgcgcg
     5341 tcggccgtca gcatgctcag gtcggtgtac agcgccgccg tgcgcgggtt gtagttgccg
     5401 gtggacaggt gcccgtagcg gcgcagctgg cggccttcgc gccgcgtcac cagcagcatc
     5461 ttggcgtggg ttttcaggcc caccacgccg tagacgacct gtgcgccggc ctgctcgagc
     5521 cgctcggccc agttgatgtt ggcctcttcg tcgaagcgcg ccttcagctc gaccaccgcc
     5581 gtcacttcct tgccgcgccg ctgcgcgtcg atcagcaaat ccatcatgcg gctgtcgttg
     5641 ccggcacggt agatggtctg cttgatggcc agcaccagcg ggtcgtgcac ggcttcgtgc
     5701 agaaaatcga tcacgccctc gaagctctcg aacggctggt ggatcagcac gtcgccccgg
     5761 cgcaggcgct cgaacaccga ttcgccacgt tgcagctgca tcgggtagct ggcgcggtac
     5821 ggcgcaaaca gcagcgccgg ctcgtcgacc agcgaaatca gctgctgcag gcgcaccagg
     5881 ttgaccgggc cctgcacctt gaacagcgcc tcgggcggca gcttgaactg gcgcagcaga
     5941 aactcggcca gcgcctgcga gcaagccgac gacacctcca cccgcaccgc ctcgccgtag
     6001 tggcgctggt gcagctcttg gcgcagcgcg gtgcgcaggt tcttgacctc ttcctcgtcc
     6061 accgacaggt cggaatcgcg cgtgacgcga aagtgcgaga agtcgcccac gtcgcggccc
     6121 ggaaataaat ccgccaggtg tgcgcggatg acgctggaga gcatgacgaa cagcgtttcg
     6181 ccccccgtta cgcgctcggg caggcgcagc acgcgcggca gcacgcgcgg caccttgacg
     6241 atggcgatct cgttgctgcg gccaaaggcg tccttgcctt tcagctgcac gatgaagttg
     6301 agcgacttgt tggcgaccag cggaaacggg tgcgccggat cgagcgccac cggcaccagc
     6361 agcggcttga cctcgcgcag gaagtactcg cgcacccatt tgcgctgcgc ctcggtgcgg
     6421 tctatgtgcg agacgatgcg catgcgccgg gtcgccatcg cgggcagcag cacctcgttg
     6481 tagatggcgt attgccgctc gaccagcgcg tgcgcggcgg ccatcaggtg gcgcatgccg
     6541 ccctgaacat cggtgccggc gcgccaggcc gtcaggtgcg gctcggcgcg cacctcgaag
     6601 aactcgtcca ggttggacga cacgatgcac aaatagcgca ggcgctccag cagcggcacg
     6661 tcgggccgct cggcccagtg cagcacgcgc aggttgaagt cgaggatgct ggcgtcgcgg
     6721 tcttgcagcg ggtagcgcgc caggatggcc tgggtgtcag cggcgtcggt cggcatggcg
     6781 gcacttcaaa aagtggagca tcaccgcaag gataccgcag ggcaaagcct agcccggcac
     6841 cgcttgcggc acgccttcag cgcgcgccgg caccaggtag ccgcgaaaca ggcgcgccgc
     6901 ttcgtcccag ccaaagcgct gggcgtgcgc gcgggccgct tcgcgcggaa tgcgcagcgc
     6961 ctgctgcacg gcgtggcgca ggtcctggtg catcacgccc cccacggcgt gaccgggcgc
     7021 ggcggccagc acctcggtcg ggccgtcgac cgggtaggcg gcgacgggcg tgccggtggc
     7081 catggcttcc agcatcacca ggccaaaggt gtcggcgcgg ctggggaaga cgaacacatc
     7141 ggccgccgcg tacacgcgcg ccagctcatc gcgcggcagc acgcccagcc agcgcacctg
     7201 cggaaagcgc gcgcgcagct tgtcttcgac cgggccgacg ccgcacacca cctgcgtgcc
     7261 gggcaagtcc agttccagaa aggcctggat gtttttctcg tacgacacgc ggccgacaaa
     7321 caggtgcagc ggccgcgcaa agcgccccag cggcgcgtag tccgctgcct tgggcgagaa
     7381 gccgaaccag cgcgtgtcga cgccgtgcgt ccattcgcgc aggttcttga agccgcgctc
     7441 ggccagcccc gccgccacgc cgcgcgtggg caccatcacg cccgacgacg ggcggtgaaa
     7501 gtggcggaag aacgcatagc cccagcccag cggcagcttg accgccgcct gcaggatttc
     7561 gggaaacttg gtgtggaacg cggtggtgaa ggcccagccg cgccgcaagc aatgccgccg
     7621 cgcggcccag ccgaggggtc cttcggtggc gatgtgcacg gcgtcgggct gcgccgaatc
     7681 gagcagctcg cgtacgcggg cgccagggcg caccgcgagg tcgatgccgg ggtaaccagg
     7741 acacggccgg gtcttgaact ggcccggctc gatgacgatg acctcgtcgc cgcccgcgcg
     7801 cagcgagttg accagctcga ccagcgtggt caccacgccg ttgacctgcg gctgccaggc
     7861 gtccgtcacc aaagcgattt tcatggcgtt gcttcaaaaa taagagctgc ttgcgctgta
     7921 tggacaagcg ctgggggctg atttggcttg aataccaaca ttcgtactcg aaggacgcga
     7981 aggttccgcg aaggacgcga aaaaagtcaa aaaaacaagc tttctctttg tctttttcgc
     8041 gtccttcgcg cagtttttgc gtccttcgcg tccagctgtt tcacaccgtt tccaccgccc
     8101 gctgggacat cacttcggcc ccatggccgg cctgccagaa caccaactcc agccgcccgt
     8161 cgtggtgctc gaccagcgcg ctgcggcttt cgacccagtc gccgtcgttg cagtacagcg
     8221 tgccgtcgac gtcgcggatt tcggcgtggt ggatgtgacc gcacaccacg ccgtcatagc
     8281 cgcgccggcg cgcctcggcg gccacggcgc cttcaaagtc gctgacgtag ttcaccgcct
     8341 tcttcacttt gtgcttcagg taggccgaca gcgaccagta cggcaggccc aggcgcgcgc
     8401 gaaggctgtt gaagtggcgg ttgagcttga gcaccatctc gtacgcgtgg tcgcccacat
     8461 aggccagcca cttggcgtgc tggatcacgc cgtcgaagta gtcgccatgc gtgatccaca
     8521 ggcgccggcc gtccacggtg acgtgcacgc tgtcttccac cacgtcgatg ccgccgaagt
     8581 tgtggttcaa aaagccgcgc gcaaactcgt cgtggttgcc cggcacgaag accacatggc
     8641 agcccttgcg cgcgcggcgc agcagctttt gcaccacgtc gttgtgcgac tgcggccagt
     8701 accagcggcg gcgcaactgc cagccgtcga tgatgtcgcc gatcaaatac agcgtttcgc
     8761 tctcgtgttc tttcaaaaaa tcgagcagcg gcccggcctg gcagcccggc gtgccgaggt
     8821 ggatgtcgga tatgaaaatg gcgcggaacc ggggtcggtc gcgcgccatg ctgggctcgt
     8881 cggcgtcatc gccgccgccc ccgggcgaca gcggccacgg cgcgtcgcga tcggccgtgc
     8941 ccatcgccgg cggcacgggg cgatccagcg gcgccaagtc gccacgcaac aggtgctggg
     9001 cggcgtggcc cagtgccgcg tcgaggttca ttgccacggg cgccatcgcc gtcatgcgcc
     9061 cagaaagtgt ttgcggtaac gcgggtgcag atcggcaaac cgcatcatca tcggcaagtc
     9121 ggcgctgttg aagtccgggt cccacgcggg cgcgcccagc accttggtgc ccaggcgcag
     9181 gtagcccttg atgagcgcgg gcggctccac cggcagccgg tcgttcaagt tttccagcgg
     9241 cagcgccagg cgcgggcgca cctggaactc gatcggcgcc atgtattgct cgcgcacctg
     9301 gcgccagatg ctggccgcca cgtcgccgtt gaacacgccg ccttgcatca tggggatgct
     9361 ggcgcagccg atcatggtgt cgaggttgtt gcgcaccatg aattcgccca gcgccgtcca
     9421 cagcgccatg atcacgccgc catggcgatg gtcggggtgc acgcagctgc ggcccagctc
     9481 gaccatgcgg tggcgcaggc tgcgcaggcg cgtcaaatcg aactcggtat cgctgtaggt
     9541 gctgcccacg cgcttggctt gcactggcgt cagcacgcgg taggtgccga tgactgtctg
     9601 cgtggcttca tcgcgcacca gcaggtgctc gcaataatcg tcgaacaggt cgatgtcgtg
     9661 gcccggcagc gtggtggcca ggcgggcgcc catctcgtgc acgaagacct ggtagcgcag
     9721 gcgctgggcg gcgcggatct cgtccagttg gcgcgcccac gacacctgga tgccgccatc
     9781 gaggccggtg gcgcccaccg ggtctcgcac aaaggcgcca ccgggcacgt gcaccgacgg
     9841 cgtggtcgaa aagccgaagc tgggcggtgt cgtttctttc acgagggagg ctcctttcct
     9901 ctttttcgtg ttcttcatgt tgcgtaactc gaatgacggg tgcgtggcag aaaaatgaaa
     9961 gattcatgac cttataccgg gtgagcggcc acacgccgtc gccatcggct tgacagatgc
    10021 atgaagttga aaccgacgtg cgtccgcacc tgcgtcgcac cttgcatcga tttgacaatt
    10081 caacaataat tgaatcatgg aacaggatca ggccattaaa gtcttcgaat cgctcgcctc
    10141 cggcctgcgg ctggacgtgt accgcctgct gatgcgcgcc ggccccgaag gcctggtggc
    10201 cggcgacatc gccagcgcgc tgaacgtgcc gccgaccaat ttgtcgtttc acctcaaggc
    10261 gctgacgcag tccggcctgc tcggcgtcac gcccgagggc cgctaccagc gctaccgcgc
    10321 caacctggcg ctgatgcagg cgatggtcga ctacctgacg gccgaatgct gcgctggtca
    10381 cccggagcag tgcgccgatt tgcgcggcga gcgggtctgc gcgccggcat cagccacaac
    10441 cgaatcgatt taagcctaaa ccggccctgg cgcccgtcga tcaagcgcca gcagctctcc
    10501 aattcatagc aatccttggc cctttatttc aaacatcgtt gaatacttca aatgaccatc
    10561 gaccgcccca ccctgaacgt gctcttcgtc tgcaccggca actcggcgcg cagcatcctg
    10621 gccgaatcca tcctcaacca cccggccatc ggccaacggc gctttcgcgc cttcagcgcc
    10681 ggcagccacc cggcgggcca cgtgcagccg ctggcgctgg ccttgctggc gcagtaccac
    10741 tacccgaccg acggactgcg cagcaagaac tgggacgagt tcagcggccc gagtgcgccg
    10801 cggctcgatt tcgtcttcac cgtctgcgac cgcgcagccg gcgaagcctg cccctactgg
    10861 cccggccagc cgatgaccgc gcactggggc gtcgaagacc cggccgccgt gcagggcagc
    10921 gccgaggagc agcgtcgcgc ctatgccaac gccttcatgg tgctgaaaaa ccgcatcgcc
    10981 atgttcgccg ccctgccctt cgacaagctg gatcggctgt cgctcaagca gcagctggac
    11041 ggcattggcg cggcggcacc ggtcgcttga tgtgaacgaa ttgctattca tccaatagct
    11101 gctcgcgctt acccaacaag cgccagaggc actttttact cagatcatca tgggactttt
    11161 tgaacgctac ctgacccttt gggtcgcgct cgccatggcc gccggcgtcg ccctcggcac
    11221 cgtgatgccc ggcatgtttc agaccatcgg ccgcatggag ctggcgcgcg tcaacctgcc
    11281 cgtgggcctg ctcatctggg tgatgatcat cccgatgctg gtcaaggtcg atttcggctc
    11341 gctcggccag gtgcgccggc acgcgcgcgg catcggagtc acgctgttcg tcaactggct
    11401 ggtcaagccg ttttcgatgg cgtttctggg ctggctgttc atccgccacg tgttcgcgcc
    11461 gtatctgccg gccgaccagc tggacagcta catcgccggc ctgatcctgc tggccgccgc
    11521 gccctgcacg gccatggtgt tcgtgtggag tcggctgacg aacggcgatg ccaacttcac
    11581 cttgagccag gtggcgctga acgacggcat catggtcgtc gcctttgcgc cgctggtggc
    11641 ttttctgctg ggcgtgtcgg ccatcacggt gccgtgggcg acgctgctgg tctcggtgct
    11701 gctgtacatc attgtgccgc tggtcatcgc gcagctctgg cggcgtgcgc tgttgtcgcg
    11761 cgggcaggct gccttcgacg ccgccatggc gagcaccggc ccgtggtcca tcggcgcact
    11821 gctgctcacg ctggtgctgc tgtttgcctt ccagggcatg caaatcgtgc gccagccgct
    11881 catcatcgcc atgctggcgg tgccgatcct gatccaggtg ctgttcaact ccgcgctggc
    11941 ctacgggctc aaccgcgcgc tgggcgaatc gcaccacgtc gcctgcccct cggcgctgat
    12001 cggcgccagc aactttttcg agctggccgt ggcggcggcg attgcgctgt tcggctttga
    12061 atccggcgcc gcgctggcca cggtggtggg cgtgttgatc gaggtgccgg tgatgctgct
    12121 ggtggtgcgc gtggtcaatg ccagcaaggg gtggtacgag cggggcgcgg ccggctcacg
    12181 ctcagcctag cgcaggccta ccgatcaggc acggaagcag cgccgttggc cgccaccagg
    12241 cccgcctgca aggccgcgcg catgtcctgg ggcagactgg ccacaccctt caccacgccg
    12301 gggccgccgt ggcgccagtg cacgatgaag ctgtgtggtt catcagcggt accgatcgga
    12361 taccgcacgg cgcggaaaac gacttccgtg ccgccgtcca ccaacgcggc ggacgacaac
    12421 agcttgacga ccggccccaa ttccagcacc tcgatgccga tcagcctgga cgagtccggc
    12481 gctgctggca ag
//
LOCUS       DAOMVO010000038        12148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1196_length_12148_cov_13.082444, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000038 DAOMVO010000000
VERSION     DAOMVO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1196_length_12148_cov_13.082444"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKV30_03440"
     CDS             complement(<1..220)
                     /locus_tag="PKV30_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HRL29308.1"
                     /translation="MQPSHSSDFLVFTGNANPALAAEIASHLNISLGAADVGRFSDGE
                     VTVEIKQNVRARDVFVVQSTCAPTNENLM"
     gene            complement(286..362)
                     /locus_tag="PKV30_03445"
     tRNA            complement(286..362)
                     /locus_tag="PKV30_03445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Gln,seq:ttg)
     gene            complement(400..1260)
                     /gene="ispE"
                     /locus_tag="PKV30_03450"
     CDS             complement(400..1260)
                     /gene="ispE"
                     /locus_tag="PKV30_03450"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRL29309.1"
                     /translation="MRSLYDVPAPAKLNLFLHITGRRADGYHLLQSAFMLIDWCDTLH
                     FESSADRLVTREDLGPPLPPDDLTLRAARALQQATGCTLGAHIRVHKRIPAQAGMGGG
                     SSDAATTLIALNRLWALNLTQTQLAAIGLTLGADVPFFIHGGHGRVEGIGEQIRPLAL
                     PPARFLVVKPPQGLDTGAIFGAPDLKRDTEPATIAGFAADPYGFGHNDLQPVAERLCP
                     QVSEALQWLTQRGLHGRMTGSGSAVFAQVPQAQVSTLATALKAPADWQVRVCSNLEHH
                     PLVGWVPRDD"
     gene            complement(1269..1784)
                     /locus_tag="PKV30_03455"
     CDS             complement(1269..1784)
                     /locus_tag="PKV30_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HRL29310.1"
                     /translation="MIGTCGLGARRALLAILAITIFLIAGCASQTGARGTFDPETSQW
                     SGRLGLTLESEPPQSFSASFDLRGNPQVGELTLTSPIGSTLAVMQWQPGEAWLRQGDQ
                     ARRYDSIDTLAQEATGTPLPVRALFGWLRGEAQSVPGWQPDLSQLANGRLNARRLMPL
                     PTAELRVILDR"
     gene            complement(1793..>2668)
                     /locus_tag="PKV30_03460"
     CDS             complement(1793..>2668)
                     /locus_tag="PKV30_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL29311.1"
                     /translation="GALLADENKDAQAEADLRHYLKLLESAAPEAAPERSEGPDHARM
                     MLARIAERRGDYAAAEQLLAAVDSPEQALTVQTRRAQILARQGKLAEAREAIRRVPER
                     SADDARLKLLAEAQLLRDNQQAALAYQLLSAEIEKTPDDDGLLYDAAMAAERANQLDT
                     MERLLRRVIELKPDAAHAYNALGYSFADRNLRLDEARTLIEKAVQLSPDDAYIQDSLA
                     WVHFRQGNVREARKLLDAAYKKRPDAEIAAHLGEVMWTQGERDAARRLWRDGLRLDPR
                     NETLLKTLERFGVTP"
     assembly_gap    2669..2768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2769..3343)
                     /locus_tag="PKV30_03465"
     CDS             complement(<2769..3343)
                     /locus_tag="PKV30_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29312.1"
                     /translation="MQFHRALGGLALACGAAAALAQTVPTPAASAPAAAASAPAAPSR
                     MTARLMYELLIGELLFRGGDAPKGTAYVLNAARRVGDESLFKRATEMAIQSRSGPAAL
                     EASRAWRQAAPNSAEAGQYELQVLIVLGRIAETEEPARRFLATLPESDKVPFITALPA
                     LYQRVPDKAEAARVVESALSDAIKQPALAPAA"
     gene            3385..4200
                     /gene="mutM"
                     /locus_tag="PKV30_03470"
     CDS             3385..4200
                     /gene="mutM"
                     /locus_tag="PKV30_03470"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRL29313.1"
                     /translation="MPELPEVEVTRRSFADRIQGARIVAAEPGLPLRWPLGRAPEDLV
                     GQTVAGVDRRGKYLLLRLDDGVLLVHLGMSGSLAFRHDLPPPGKHDHFDLITTRGTLR
                     LHDPRRFGAVVWSPGLDADPARKLLSTLGVEPLDGAFDAAAFHTGLRRRRAPIKQVLL
                     AGDVVVGVGNIYASEVLFMAGIRPTTRADRLSRARSDRVHAAVRQVLARAVERGGSTL
                     RDFSSAVGESGHFQLEAAVYGRAGQPCVVCATPLRQIRQGQRSTFFCPVCQRP"
     gene            4301..6268
                     /locus_tag="PKV30_03475"
     CDS             4301..6268
                     /locus_tag="PKV30_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRL29314.1"
                     /translation="MAVAGFNEQFDKHGAWRREVGLRLKVLTDWLKEQQLLDDGADES
                     VQRLIEQLRSDKVMVAFVAEFSRGKSELINAVFFAGYGRRIMPASAGRTTMCPTELGY
                     DADLPPCLRLLPIETRLQPQALLEWRMVPEQWTRVNLDVNNPEQLAGAMEKVSEVLHV
                     TRDEARALGFWHDDRPEDNPPVNAAGAVEVPRWRHALINMAHPLLRQGLVVLDTPGLN
                     AIGAEPELTVNLIPQAQAVVFILGADTGVTKSDLTIWREHLATMEGSEGTRFVVLNKI
                     DTLWDELSTPAQIEAQMLRQCAETAQTLALNGEQVLALSAQKGLLAKVRGDTALLEQS
                     RLPTFESLLGQQVLGARQAMLEGAIRTGVGRVQSDAQRTIGVRRRELAEQMMELKGLR
                     GKNHQVIRQMRVRVETEKADFDRSAASVLAVRSVHLKQLRDVFKLLGSTSIKRELAGL
                     STALREPGLKLGIKKSYAKGFDNLRGVVRQVQLLEGEIHSMLGTSFRQLNTEYGFSLQ
                     AADPPAMDRFEKDLDAVERSHLQYLGVGNMMQLARPEFAEKLVRALLARVRSIFETAL
                     GEIELWNKSTSGQLDAQLRERRRAYGRRIEAIQRIQDAAGGLDLRLGEIGEQEAALDA
                     VTQRLQSMSHELLAARADAGARAEAAEAVAV"
     gene            6265..7311
                     /gene="mutY"
                     /locus_tag="PKV30_03480"
     CDS             6265..7311
                     /gene="mutY"
                     /locus_tag="PKV30_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRL29315.1"
                     /translation="MSRSDGDFSTRIIAWQQRHGRHDLPWQGTRDPYRVWLSEIMLQQ
                     TQVATVLAYYPRFLARFPDVARLAEADEDEVLGLWSGLGYYSRARNLHRAAREVMARF
                     GGAFPATAAELATLPGIGRSTAAAIAAFCFGERAAILDGNVKRVLTRAFGIADDLSEA
                     RHERALWQRAEALLPTTDPARHMPIYTQGLMDLGASICSQRRPACTVCPLVDVCVAAR
                     AGEPERYPVKTRRTARRQATLWLLHAQAGQGRVWLVQRPAKGIWARLFCLPLFESDEA
                     LMASLPASARADAVLWPAFRHVLTHRDLDIHIVSARLGAHDLADAAGRWFGADEWPAL
                     GLPAPIRRFLQQAA"
     gene            complement(7521..8384)
                     /gene="rapZ"
                     /locus_tag="PKV30_03485"
     CDS             complement(7521..8384)
                     /gene="rapZ"
                     /locus_tag="PKV30_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRL29316.1"
                     /translation="MSMDLVVITGMSGSGKSIALGALEDAGYYCVDNLPPELLEPFLA
                     LEDRLRARKVAIAMDVRSGESLPQLPLLLHGLRDKGLSLRQIFLDANDHTLVRRFSET
                     RRRHPLSTPGETDIQQALLQTIRRERELLADLRERSHVIDTSYLRPSQLLAMVKDLIA
                     APAAHLTLVFESFAFKRGITLDADYVFDVRMLPNPHYEPELKPLTGRDQPVIDFLARQ
                     PAVQLMQEQIATFLRTWLPALAHDHRSYVTVAIGCTGGQHRSVYLVEQLAAQFGQDWQ
                     TLRRHRELDGL"
     gene            complement(8381..10117)
                     /gene="recN"
                     /locus_tag="PKV30_03490"
     CDS             complement(8381..10117)
                     /gene="recN"
                     /locus_tag="PKV30_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRL29317.1"
                     /translation="MALRHLNLRDFVIVPALDLALGGGFTALTGETGAGKSILIDALQ
                     LVLGGRGDALWVREGQPRCEIAAEFDLPDSARAWLAEQGFDDESAAEAGTLLLRRTID
                     AAGKSRAWVNGSPATLAQLRALGEWLVDIHGQHAWQSLTRPAAVRELLDAYAGTDPKP
                     LAAAWQAWRQADAALQTARAAQDSLTEERERLLWQIAELDKLAPADGEWDELNQRHAR
                     LSHAQALIDAGHAAATALEGGDDGEGGALPSLERALTTLADQRAIEPEFAALADVLDT
                     CIAQARDAAHSLHAYLRRADLDPEQLAALDERMGQWMGLARRFRRPPEELAAVLAAWR
                     ADLARLESASDLAQLQAVADAARQTWLQAAQAVSRQRQQAAPRLSAAVSAAMQDLGMQ
                     GGRFEVALQPLAEPAQHGLENVLFQVAAHTGSAPRAIDRVASGGELSRLALAIAVTTS
                     ALGSVGTLVFDEVDSGVGGAVAATVGRLLQRLGRDRQVLCVTHLPQVAACADAHLLVS
                     KQAAADGAPTSTVAPIAGEARTREVARMLGGERISDTTLAHALEMLNAPAADDAAPSP
                     TPRGGARKARSP"
     gene            complement(10122..11018)
                     /locus_tag="PKV30_03495"
     CDS             complement(10122..11018)
                     /locus_tag="PKV30_03495"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRL29318.1"
                     /translation="MSLQFHRVALVGKYQDAASGAVADSTRDVIADVAGFLTEQGCDV
                     VLAERTAEATGLRDHPALDVAGIGAECDLAVVVGGDGTMLGIGRQLARYGTPLIGINQ
                     GRLGFITDLPLGDYRQTLPPMLHGAYEEDHRSLMLGKVMRGGECVFEAYAMNDVVVNR
                     GAVASMVELRVEVDGHFVANHRADGIIVATPTGSTAYALSAGGPLLHPLVPAWVLVPI
                     APHTLSNRPIVLADPVEIVIEVVGGREASASFDMQSITSLQHGDRIIVRRSDYRVRFL
                     HPRGWSYFDTLRAKLHWNEGGV"
     gene            11045..12064
                     /gene="hrcA"
                     /locus_tag="PKV30_03500"
     CDS             11045..12064
                     /gene="hrcA"
                     /locus_tag="PKV30_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRL29319.1"
                     /translation="MLDDRSRLLLKTLIERYIADGQPVGSRTLSRAPGLELSPATIRN
                     VMSDLEDLGLIASPHTSAGRIPTARGYRLFVDTMLTAQQGELGTPTLAAEQPQKVIAN
                     AAHLLSNLSQFVGVVMAPRRSSAFRHIEFLRLSERRLLLIMVAPDGDVQNRVLFTDAD
                     YTASELQQASNYLNAHFSGMDIEQVRLRLRQEVDTLRGEIATLMRAAVQVGSEAMSQA
                     QGEVIISGERNLLAVSDFSSDMNQLRRAFDLFEQKAQLMRLLDVSSQAEGVRIFIGGE
                     SQTVPVEDLSVVSAPYEVDGQVVGTLGVIGPTRMPYNRMIQIVDITSRLVSNALSQGK
                     PGGTA"
BASE COUNT         1885 a         4079 c         4171 g         1913 t
ORIGIN      
        1 ccatcaggtt ctcgttggtc ggcgcgcagg tggactgcac cacgaacacg tcgcgcgcac
       61 gcacgttctg cttgatctcg accgtgacct cgccatcgga aaagcggccc acgtcggcgg
      121 cgcccaggct gatgttgagg tgactggcga tttccgccgc caacgcgggg ttggcattgc
      181 cggtgaacac cagaaagtca gaagaatggg acggctgcat gcgctttccg gcgggtcact
      241 gaagtgcggc tcagtgccag gcgcagcgca cccggcgaaa tggtttggca ggggaggaag
      301 gactcgaacc ctcgcatgcc ggaatcaaaa tccggtgcct tgaccaactt ggcgactccc
      361 ctacaccggt gatgggcctg aactgcgggc caaccacctt caatcgtcgc gtggaaccca
      421 gccgaccaac ggatggtgtt ccagattgct gcacacgcgt acttgccagt cggccggtgc
      481 cttgagcgcg gtggccaaag tggacacttg cgcttgcggc acctgcgcga aaaccgcgct
      541 gcccgagccg gtcattctgc cgtgaaggcc ccgttgtgtt aaccactgga gcgcctcact
      601 cacctgcggg caaagccgct cggcaaccgg ttgcaggtcg ttgtggccaa agccgtacgg
      661 gtctgcagca aagccagcaa ttgtagcagg ctcggtatcg cgcttcaggt caggggcgcc
      721 aaaaattgcg ccagtatcga gcccttgcgg cggttttacg acaagaaacc gggccggcgg
      781 cagcgccaag gggcgaatct gctcgccgat gccctcgacc cggccatggc cgccgtggat
      841 gaaaaacggc acgtcggcgc ccagcgtcag gccgatggcg gccaactgcg tttgcgtgag
      901 attcagcgcc cacaggcgat tgagcgcgat cagcgtggtc gccgcgtcgg acgagccgcc
      961 gcccatgccg gcctgcgccg ggatgcgctt gtgcacacgg atgtgcgccc ccagcgtgca
     1021 gcccgtggcc tgctgcagcg cgcgtgcggc ccgcagcgtc aggtcgtcgg ggggcagcgg
     1081 cggccccagg tcttcgcgcg tgacgaggcg gtccgcgctc gattcgaaat gcagggtgtc
     1141 gcaccagtcg atcagcatga aggccgactg cagcaggtga taaccgtcgg cgcgccggcc
     1201 ggtgatgtgc agaaacagat tgagcttggc cggcgccggc acgtcgtaca gcgagcgcat
     1261 cgcgggcgtc agcggtcgag aatgacgcgc aattcggccg tcggcagcgg catcaggcgg
     1321 cgggcgttca ggcgcccgtt ggccagctgg ctcagatcgg gctgccagcc gggcacggat
     1381 tgcgcttcgc cgcgcagcca gccgaacagc gcgcgcaccg gcagcggtgt gccggtggct
     1441 tcctgcgcca gcgtgtcgat cgagtcgtag cggcgcgcct gatcgccctg gcgcaaccac
     1501 gcttcgccgg gctgccactg catgacggcc agcgtgctgc cgatgggcga ggtcagtgtc
     1561 agctcgccga cctgcgggtt gccgcgcaaa tcgaaactgg cggaaaagga ctgcggcggc
     1621 tcggattcga gggtcaaccc aaggcggccg ctccactgac tggtttctgg gtcaaatgtg
     1681 cctctggcgc ccgtctggct tgcgcaacca gctatcaaaa aaatagtgat cgccaagatg
     1741 gccagcagcg cacggcgcgc gcccagtccg catgtcccga tcaccgcacg attcaaggcg
     1801 tcaccccgaa gcgctcgagc gttttcagca gcgtctcgtt gcgcgggtcc aggcgcagac
     1861 cgtcgcgcca caggcgtcgc gcggcgtcgc gctcgccctg cgtccacatc acctcgccca
     1921 aatgggcggc gatctcggcg tcggggcgct tcttgtacgc ggcgtccagc agcttgcgcg
     1981 cctcgcgcac gttgccttgc cgaaagtgca cccaggccag gctgtcctgg atgtaggcgt
     2041 cgtcgggcga cagttgcacg gccttttcga tcagcgtgcg ggcttcgtcg agacgcaaat
     2101 ttcgatccgc aaacgaatag ccgagcgcgt tgtaggcgtg ggcagcatcc ggcttgagct
     2161 cgatcacgcg gcgcaacagg cgctccatag tgtcgagctg gttggcgcgt tcggcggcca
     2221 tggcggcgtc gtagagcaga ccgtcgtcgt cgggcgtttt ctcgatttcg gcgctcagca
     2281 actggtaagc cagcgcggcc tgctggttgt cgcgcagcag ttgcgcttcg gccagcagct
     2341 tcaggcgcgc gtcgtcggcg ctgcgttcgg gcacgcggcg aatcgcctcg cgcgcctcgg
     2401 ccagcttgcc ctggcgcgcc agaatctgcg cgcggcgcgt ttgcacggtc agcgcttgct
     2461 cgggcgaatc gacggcggcg agcagttgct cggccgccgc gtagtcgccg cgccgctcgg
     2521 caatgcgcgc cagcatcatg cgggcgtggt cggggccttc agaacgttcg ggtgcagcct
     2581 ccggggcggc gctttccaga agcttcaggt agtgccgcag atctgcctcg gcctgcgcgt
     2641 ccttgttttc atcggccaac agcgcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnngc cgcgggcgcc agcgccggct gcttgatggc gtccgacagc gcgctttcga
     2821 ccacgcgagc ggcctcggcc ttgtccggca cgcgctggta aagcgcgggc agcgcggtga
     2881 tgaagggcac cttgtcgctc tccggcagcg tggccagaaa gcgccgcgcc ggctcttcgg
     2941 tttcggcgat gcggcccaga acgatcagca cctgcagctc gtactggccg gcttcggccg
     3001 agttgggggc agcctggcgc caggcacggc tggcttccag cgcggcgggg ccagaacgcg
     3061 actgaatcgc catctcggtg gcgcgcttga acagcgattc gtcgcccacg cggcgcgcgg
     3121 cgttgagcac gtaggcggtg cctttgggcg catcgccgcc ccgaaacagc agctcgccaa
     3181 tcagcagctc gtacatcagc cgcgcggtca tgcgcgaagg ggcggcgggg gcggaagcag
     3241 cggcggccgg tgcgctggcg gctggcgtcg gcacggtctg cgcgagcgcg gcggccgccc
     3301 cgcaagccag cgccaagccg cccaaggcgc ggtgaaattg catcatcgac tcataataat
     3361 ccatgcccat tgatgcgttg tgccatgccg gaattgcctg aagtcgaagt cacccgacgc
     3421 agttttgccg accgcattca gggcgcgcgc atcgtggccg ccgagcccgg cttgcccttg
     3481 cgctggccgc tgggccgggc gccggaagac ctggtggggc agacggtggc gggcgtcgac
     3541 cggcgcggca agtacctgct cctccggctc gacgacggtg tgctgctggt gcacctgggc
     3601 atgtcgggca gcctggcctt tcggcacgat ttgccgccgc ccggcaagca cgaccacttc
     3661 gacctcatca ccacccgcgg cacgctgcgc ctgcacgacc cacgccgctt tggcgccgtg
     3721 gtctggtcgc cgggcctgga tgccgatccg gcgcgcaagc tgctgtccac gctgggcgtg
     3781 gagccgctgg acggcgcttt cgatgccgcc gccttccaca ccgggctgcg ccggcgtcgc
     3841 gcgccgatca agcaggtctt gctggcgggc gacgtggtgg tgggcgtggg caacatctac
     3901 gcgtccgagg tgctgttcat ggccggcatc cgcccgacca cgcgcgccga ccggctgtcg
     3961 cgcgcgcgct cggacagggt gcacgcagcg gtgcggcagg tgctggcgcg cgcggtcgag
     4021 cgcggcggca gcacgctgcg cgacttttcc agcgcggtgg gcgaaagcgg gcatttccag
     4081 cttgaagccg ccgtctatgg ccgtgcgggg cagccctgtg tggtttgcgc cacgccgctc
     4141 aggcaaatcc ggcagggcca gcgctccacc ttcttctgcc cggtgtgcca gcgcccataa
     4201 gcaccattag gtcacgcctg cgccgcctgg ggcgcttgcc ggacggccgc aagccggggg
     4261 tcgcggtgta atatgccgcg aactccggga gtggctcaac gtggcggtcg caggtttcaa
     4321 cgagcagttt gacaagcacg gcgcgtggcg ccgcgaggtg gggctgcgcc tgaaggtgct
     4381 gaccgactgg ctgaaagagc agcaattgct ggacgatggc gccgacgaaa gcgtgcagcg
     4441 cctgatcgag cagttgcgct ccgacaaggt catggtcgcc ttcgtggccg agttttcgcg
     4501 cggcaagtcg gaactgatca acgccgtctt ctttgccggc tacggccgcc gcatcatgcc
     4561 cgccagcgcc ggccgcacca ccatgtgccc gaccgagctg ggctacgacg ccgacctgcc
     4621 gccctgcctg cgcctgctgc cgattgaaac gcgtctgcag ccgcaagccc tgcttgaatg
     4681 gcgcatggtg cccgagcagt ggacgcgcgt caacctcgac gtgaacaacc ccgagcagct
     4741 ggccggcgcg atggagaaag tctccgaagt gctgcacgtc acgcgcgatg aggcgcgcgc
     4801 gctcggcttc tggcacgacg accggcccga ggacaacccg cccgtcaacg ccgctggcgc
     4861 cgtcgaagtg ccgcgctggc gccacgcgct catcaacatg gcgcacccgc tgctgcgcca
     4921 gggcctggtc gtgctcgaca cgccgggcct gaacgccatc ggcgccgagc ccgagctgac
     4981 agtcaacctg attccgcagg cgcaggcggt ggtgttcatc ctgggcgccg acaccggcgt
     5041 caccaagtcc gacctgacca tctggcgcga acacctggcc accatggaag gcagcgaggg
     5101 cacgcgcttc gtggtgctca acaagatcga cacgctgtgg gacgaactca gcacgccagc
     5161 gcagatcgag gcgcagatgc tgcgccagtg cgccgaaacc gcgcaaacgc tggcgctgaa
     5221 tggcgagcag gtgctggcgc tgtcggcgca aaagggcttg ctggccaagg tgcggggcga
     5281 cacggctctg ctggagcaaa gccgcctgcc gacgtttgaa tccttgctgg gccagcaggt
     5341 gctgggcgcg cgccaagcga tgctggaagg cgccattcgc accggcgtcg gccgcgtgca
     5401 gtccgacgcc cagcgcacca tcggcgtgcg ccggcgcgag ctggccgagc agatgatgga
     5461 gctcaagggc ctgcgcggca agaaccacca ggtgattcgc cagatgcgcg tgcgcgtgga
     5521 gacggaaaag gccgatttcg accgcagcgc cgccagcgtg ctggccgtgc gctcggtgca
     5581 cctgaagcag ttgcgcgacg tgttcaagct cctgggcagc acgtcgatca agcgcgagct
     5641 ggcgggcttg agcacggcgc tgcgcgagcc ggggctcaag ctcggcatca agaagtcgta
     5701 cgccaagggc ttcgataacc tgcgcggcgt ggtgcgccag gtgcaactgc tggaaggcga
     5761 aatccattcc atgcttggca cctcgttccg ccagctcaac accgaatacg gtttttcgct
     5821 gcaggccgcc gacccgcccg ccatggaccg tttcgagaaa gacctggacg cggtcgaacg
     5881 cagccacctg cagtacctgg gcgtcggcaa catgatgcag ctggcgcgac ccgagtttgc
     5941 cgaaaagctg gtgcgggcct tgctggcgcg cgtgcgctcg atcttcgaga cggcgcttgg
     6001 cgagatcgag ctgtggaaca agtccacctc tggccagctg gatgcgcagt tgcgcgagcg
     6061 ccgccgtgcc tacggccgcc gcatcgaggc gatccagcgc attcaggacg ccgctggcgg
     6121 gctggatctg cgcctgggcg aaatcggtga gcaggaagcg gcgctggatg ccgtcacgca
     6181 gcgcctgcaa agcatgagcc atgagctgct ggctgcccgg gccgacgccg gcgcgcgcgc
     6241 cgaagccgcc gaggccgtgg cggtgtgagc cgctccgacg gcgacttttc cacccgcatc
     6301 atcgcctggc agcagcgcca cgggcggcac gacctgcctt ggcagggcac gcgcgacccg
     6361 tatcgcgtgt ggctgtccga aatcatgctg cagcaaacgc aggtggcgac ggtgctggct
     6421 tactacccgc gctttctggc gcgctttccc gacgtcgccc gtctggctga agccgatgaa
     6481 gacgaggtgc tcggcctgtg gagcggcctg ggctactact cgcgcgcgcg caacctgcac
     6541 cgcgcggcgc gcgaggtcat ggcgcgtttt ggcggcgcgt ttccggcaac ggcggccgaa
     6601 ctcgccacgc tgccgggcat tggccgttcg acggcggcgg ccattgctgc gttctgcttt
     6661 ggcgagcgcg cggccatcct ggacggcaac gtcaagcgcg tgctgacgcg tgccttcggc
     6721 atcgccgatg acctgagcga agcacgccac gagcgcgccc tgtggcagcg cgccgaagcc
     6781 ctgctgccca ccaccgaccc ggcgcgccac atgccgatct acacgcaggg cctgatggac
     6841 ttgggcgcca gcatctgcag ccagcggcgg ccggcctgca ccgtgtgccc gttggtcgat
     6901 gtgtgcgtgg cagcgcgtgc cggcgagccg gagcgctacc cagtcaagac gcggcgcacc
     6961 gcgcgccgcc aggcgacgct gtggctgctg catgcacagg ccggccaagg ccgcgtctgg
     7021 ctggtgcaac ggccggccaa gggcatctgg gcgcggctgt tctgcctgcc gctgtttgaa
     7081 agcgacgagg ccttgatggc cagcttgccg gcgtcggcgc gggccgacgc ggtgctgtgg
     7141 ccggcttttc ggcacgtgct gacgcaccgc gatctcgaca tccacatcgt cagcgcgcgg
     7201 ttgggggcgc acgacttggc cgatgccgcc gggcgctggt ttggtgccga cgaatggcca
     7261 gcactggggc tgccggcgcc gatccgcagg ttcttgcaac aggcggcttg atgctgcgaa
     7321 ttcaatagct gcttgcgctt gctgggcaag cgctgggggc ttgaaatgct aaaaaaacgg
     7381 ccatccaacg gctttgaacg accttgggca cgccgcccag gcgttccaca acgctccaca
     7441 ggcccggttt caatgccttt caggccgcca gcgcttgccc agcttgcgca aggtgcttca
     7501 ataatcgaag caaggcggcg ctacaaccca tccagctcgc ggtgccgccg cagtgtctgc
     7561 cagtcctggc cgaactgtgc ggccagttgt tcgaccaggt agaccgagcg gtgctgcccg
     7621 ccggtgcagc caatcgccac cgtcacgtag ctgcggtggt cgtgcgccag cgcgggcagc
     7681 caggtgcgca agaaggtggc gatctgctcc tgcatcagct gcacggccgg ctggcgcgcc
     7741 aggaagtcga tcaccggctg gtcgcgcccg gtcagcggtt tcaactcggg ctcgtaatgc
     7801 gggttgggca gcatgcgcac gtcgaacacg tagtccgcgt cgagcgtgat gccgcgcttg
     7861 aaggcgaatg attcgaacac cagcgtcagg tgcgcggccg gcgcggcgat cagatctttc
     7921 accatggcca gcaactgcga cggccgcaaa tagctggtat cgatgacgtg cgagcgctcg
     7981 cgcaaatcgg ccagcaattc gcgctcgcgc cgaatggttt gcagcagcgc ctgctgaatg
     8041 tcggtctcgc ccggcgtcga caaggggtgg cgccgccgcg tctcggaaaa gcggcgcacc
     8101 agcgtgtggt cgttggcgtc aagaaaaatc tgccgcagcg acaagccctt gtcgcgcagc
     8161 ccgtgcagca gcaagggcag ttgcggcagc gattcgccgc tgcgcacgtc catcgcaatc
     8221 gccaccttgc gcgcgcgcag ccggtcttcg agcgccagga atggctccag cagttcgggc
     8281 ggcaggttgt cgacgcagta gtagccggcg tcttccagcg cgcccagcgc aatcgacttg
     8341 cccgagccgg acatgccggt gatgacgacc aggtccatgc tcatggcgag cgggcttttc
     8401 gcgcgccgcc gcgcggcgtg gggctcggcg cggcgtcatc ggccgccgga gcattcagca
     8461 tttcaagcgc gtgcgccagc gtggtgtcgg agatgcgctc gccgcccagc atgcgcgcca
     8521 cctcgcgcgt gcgcgcttcg ccggcgatgg gcgccacggt gctggtcggc gcaccatcgg
     8581 ccgccgcctg cttgctgacc agcaggtgcg cgtcggcaca cgccgcgacc tgcggcaggt
     8641 gggtgacgca cagcacctgc cggtcgcgcc ccagccgctg caacaaccgg ccgacggtgg
     8701 cggccacggc gccgccgacg cccgaatcga cctcgtcgaa caccagcgtg ccgacgctgc
     8761 ccaaagcgct ggtcgtgacg gcaatggcca gcgccaggcg cgacagctca ccacccgacg
     8821 ctacacgatc aatagcgcgt ggcgctgatc cagtgtgcgc tgcgacctga aacagcacat
     8881 tttccaggcc gtgctgcgcc ggttcggcga ggggctgcag cgcgacttca aagcggccgc
     8941 cctgcatgcc caagtcctgc atggcggcgc tgacggccgc cgacaggcgc ggcgccgcct
     9001 gctggcgctg gcgcgacacc gcttgcgcgg cctgcagcca ggtttggcgc gcggcgtctg
     9061 ccacggcttg caattgcgcc aggtcgctgg cggattccag ccgcgccaga tcggcacgcc
     9121 acgcggccag taccgcggcc aattcttccg gcggacggcg aaagcgccgc gccaggccca
     9181 tccactggcc catgcgctcg tcgagcgcgg cgagttgttc ggggtcgagg tcggcgcggc
     9241 gcaggtaggc gtgcaggctg tgcgcggcgt cgcgcgcctg cgcgatgcag gtgtccagca
     9301 cgtccgccag ggcggcaaat tcgggctcga tggcgcgctg atcggccagc gtcgtcagcg
     9361 cgcgctccag cgagggcagg gcgccgccct cgccatcgtc gccgccttcc agcgccgtgg
     9421 cggcggcgtg cccggcgtcg atcagcgcct gcgcgtgcga caggcgcgcg tggcgctggt
     9481 tcagctcatc ccattcgccg tcggccggcg ccagcttgtc gagttcggcg atttgccaca
     9541 gcaggcgctc gcgctcttcg gtcagactgt cttgcgcggc gcgcgcggtt tgcagggcgg
     9601 catcggcctg gcgccaggct tgccaggcgg cggccagggg cttgggatcg gtgccggcgt
     9661 aggcgtcgag caattcgcgc acggcggccg gccgcgtcag gctttgccag gcgtgctggc
     9721 cgtgaatgtc gaccagccat tcacccagcg cgcgcaactg cgccagcgtg gccggcgagc
     9781 cgttgaccca ggcgcggctt ttgcccgcgg cgtcgatggt gcggcgcagc agcagcgtgc
     9841 cggcttcggc ggccgattcg tcgtcgaagc cttgttcggc cagccaggcg cgagcggaat
     9901 cgggcaggtc gaattcggcc gcgatctcgc agcgcggctg gccttcgcgc acccacagcg
     9961 cgtcgccgcg cccgcccagc accagttgca gcgcgtcgat caggatcgac ttgcccgcgc
    10021 ccgtctcgcc cgtcaaggcc gtgaagccgc cgcccagcgc caggtccaac gcgggaacga
    10081 tgacgaaatc acgcaggttc agatgtcgca acgccatgcc gtcacacgcc tccctcgttc
    10141 cagtggagtt tggcgcgcag cgtgtcgaaa tagctccagc cccgggggtg cagaaagcgc
    10201 acgcggtagt ccgagcgccg cacgatgatg cggtcgccat gttgcagcga ggtgatggat
    10261 tgcatgtcaa agctggcgct ggcttcgcgc ccgcccacga cttcaatgac gatttccacc
    10321 gggtcggcca gcacgatggg ccggttcgac aaggtgtgcg gcgcaatcgg caccagcacc
    10381 caggccggca ccagcgggtg cagcagcggg ccgccggccg acagcgcgta ggcggtcgat
    10441 ccggtcggcg tggcgacgat gatgccgtcg gcgcggtggt tggcgacaaa gtggccatcc
    10501 acctcgaccc gcagctcgac catgctggcc acggcgcctc ggttgaccac cacatcgttc
    10561 atggcatagg cctcgaacac gcattcaccg ccgcgcatca ccttgcccag catcaggctg
    10621 cggtggtctt cctcgtacgc gccgtgcagc atgggcggca gcgtctggcg gtagtcgccc
    10681 agcggcaggt cggtgatgaa gcccagtcgc ccctggttga taccgatcag aggcgtgccg
    10741 tagcgcgcca gctggcggcc aatgcccagc atggtgccgt cgccgcccac caccacggcc
    10801 agatcgcatt cggcgccgat gccggcgacg tcgagcgcgg ggtggtcgcg caggcccgtg
    10861 gcctcggccg tgcgctcggc cagcaccacg tcgcagcctt gttccgtcaa aaaacccgcc
    10921 acgtcggcaa tgacgtcgcg ggttgaatcg gccaccgcgc ccgatgcggc atcctggtat
    10981 ttgccgacca gcgcgacacg gtggaactga aggctcatcg gcaaattaca tcataatgat
    11041 ttgaatgctc gacgaccgtt ctcgcttgct gttgaagacg ctgatcgagc gctacatcgc
    11101 tgacgggcaa cccgtcggct cgcgcacgct gtcgcgcgcg ccggggctgg agctgtcgcc
    11161 cgccaccatc cgcaacgtga tgtcggatct ggaagacctg gggctgatcg ccagcccgca
    11221 cacctccgcc ggccgcatcc ccaccgcgcg cggctatcgc ttgtttgtcg acaccatgct
    11281 gacagcgcag cagggcgagc tgggcacgcc caccctggcg gccgagcagc cgcagaaggt
    11341 gattgccaac gccgcgcacc tgctgtccaa cctgtcgcag ttcgtcggcg tggtgatggc
    11401 gccgcgccgc tcgtcggcct tcaggcacat cgagtttttg cggttgtccg agcggcgcct
    11461 gttgctcatc atggtggcgc ccgatggcga cgtacaaaac cgcgtgctgt tcaccgatgc
    11521 cgactacacc gccagcgagc tgcagcaggc gtcgaactac ctcaacgcgc atttctcggg
    11581 catggacatc gagcaggtgc gcctgcgcct gcggcaagag gtcgacacgc tgcgcggcga
    11641 aatcgccacg ctgatgcgcg cggcggtgca ggtcggctcc gaagccatga gccaggcgca
    11701 gggcgaagtc atcatctccg gcgagcgcaa cctgctggcg gtgagcgatt tctccagcga
    11761 catgaaccag ctgcgccgcg cgttcgatct gttcgagcag aaagcccagc tgatgcgcct
    11821 gctcgacgtc tccagccagg ccgagggcgt gcgcatcttc atcggcggcg agagccagac
    11881 cgtgccggtg gaagacctgt cggtggtcag cgcgccgtac gaggtcgacg gccaggtggt
    11941 cggcacgctg ggcgtcatcg gccccacgcg catgccctac aaccgcatga tccagatcgt
    12001 cgacatcacc tcgcgcctgg tcagtaacgc gctcagtcag ggcaagccgg gcggcaccgc
    12061 ctagaatcgc tggcttggcg ggccgttggc tcagttggtt agagcagggg actcataatc
    12121 aagtggtctg tggcattaat gacactag
//
LOCUS       DAOMVO010000039        11996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1229_length_11996_cov_12.403651, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000039 DAOMVO010000000
VERSION     DAOMVO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11996
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_11996_cov_12.403651"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKV30_03505"
     CDS             complement(<1..425)
                     /locus_tag="PKV30_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRL29320.1"
                     /translation="MHTLWKYLKPQWRLALVAMGLAAASQVLALIDPIIFGKLIDGYA
                     TQKGAKPDDELVRGALMLLALAVGVALASRATRALQDYAVRMVVQRFGTQIFNDGLRQ
                     TMRLNFQEYEDARSGETLSLLLRVRQDTERFINAFINVL"
     gene            complement(555..1235)
                     /locus_tag="PKV30_03510"
     CDS             complement(555..1235)
                     /locus_tag="PKV30_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRL29321.1"
                     /translation="MSSPPPTLEMETGANPRASIIVMHGLGADGSDFAPFVDEIDLAA
                     IGPVRWLFPNAPQIPVSINGGYVMPAWYDIAADRSREDEAGLRRSQASIDALLAREQA
                     RGVPAHRIVLGGFSQGCAMALLTGLRYPQRLAGILGMSGYLPLAGALAAERSSANADA
                     PIFLAHGTQDEMIPLPRAAASRDALAALGYAVEWHQYPMGHSVCAPEVQDVQRWLLRV
                     LDPAVDKK"
     gene            complement(1353..3998)
                     /locus_tag="PKV30_03515"
     CDS             complement(1353..3998)
                     /locus_tag="PKV30_03515"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HRL29322.1"
                     /translation="MQAVSTQSPLIKRARQEAVFLSSSLIDRCVEAALAALEGAERSA
                     KSVALRLQLGEAMAALTKRRAELRAAFPGQVDQAVGEALMSAREVPASGARAPVADDD
                     LALVEDAEVARFVEASRLQQTVMPVVEQNLSRLDSLMSSALGLPVVRAELNPLRPDVL
                     CGALLQLLGHQPEDPELRAHWARHMAKPFAGELNRLYESVAQLLEDQGVEEARYRLKL
                     TEGGALPRGSAKVDDAAGGSGSGAGAGGGAGSGAGGGGAMTSDADAVRQRRALFPRMS
                     DLAHAQPAVPRALMHEFLYRPQWLAENDEPLPASYYEAVDAQAQRVAQAPAPQYDAAT
                     AARTRARAQAQSVVDRAPREVAPDTPLAPEQWGEAASPQARAQTLLQLKAQAGKISQV
                     LGLDAVRTLVGQVAGDQRVLAPVREAFVAMEPALLRMAMHDPRFFGDDHHPARRLIEG
                     VAQRSFKYNDEFAPEFEQFMAPVRQAVRELNQQTEARADNFNARLQSLEADWQQQDTS
                     DERAREDGLRSMHFAQQRQALADKVAWEFSLRSDLDRVPAVVADFLFKDWSLVIAHAQ
                     LTDQRGQLDPGGYLAVVTDLLWSVKREAALKAPARLFEVVPGLVQTLRRGLQMLGKEP
                     HETETFFDALMRHHDPVLRLRRLRSARDAEASGYSRLADESARLPLETDPAPLERPQP
                     RAAEQPWLGRHELAAAGFEELSEGESAQAPLTESAPLEDESVQPSDNDDFASTEPLEP
                     AQEPTHVPAQVEGFVPLDLSMADVVKPPAAAPRAAAAKAAETPEDIEARTRAQLARLR
                     TGDWVDLKVRGHWRRAQLHWTSENGTLFMFISRGGRPHSMTRRTCEKLIRERQLRPVD
                     AGAVVDKALRQLGEAARAEREPALA"
     gene            complement(4134..4553)
                     /locus_tag="PKV30_03520"
     CDS             complement(4134..4553)
                     /locus_tag="PKV30_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL29323.1"
                     /translation="MSTVAPADDAHPDARVRAVFDDIRTTRGSDFINNFWRYLAFDAD
                     LLEATWAEVKRVMATPSALDPLTKEMIYTAVSVANGCGYCVHSHSAAARAKGMSAAQY
                     AELLAIVGLAGKTNHLVTALQVPVDPIFDADATVRQS"
     gene            complement(4620..5582)
                     /locus_tag="PKV30_03525"
     CDS             complement(4620..5582)
                     /locus_tag="PKV30_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRL29324.1"
                     /translation="MDHSTPALEVVPRDLSAYRAGNTGIDYVHRFDSGTAGPHVLINA
                     LTHGNEICGMVAATHLLDNGVRPRIGTLTVSFAHVEAYEAFQRDAPFANRQLVHNLNR
                     IWSDEWLDGPQNGPELRRARALRPVVAAADHILDIHSTSQPVAPFWVYPNFARNARAA
                     LALGALGRVGTHLVMPSGLGSGTPLIQHGRHGLDGDAAGVALVVECGQHFLRASAELA
                     TATALDFLAHFGLIEPAPRGVSAAPQRRFELLQTCMVHTPDFKFARPLIGFETFAEGE
                     LIATDGDAGEIRAPCDDCTVLMPAREPIVGREAVYLTRPIRVSA"
     gene            5644..6246
                     /locus_tag="PKV30_03530"
     CDS             5644..6246
                     /locus_tag="PKV30_03530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRL29325.1"
                     /translation="MTNALPAHVHPVELKHAYRLLNTGASILISSAHGGRGDVMACAW
                     NMALDFTPAKLAVCLDKATLTRTLVEASGEFAIGVPCAAQADLVFSVGSVSGHDLREK
                     DKFSAFNIDHFSASRVSAPLVSGCIGWLECRLIPEPHIQQAHDLFVAEAVAAWADERA
                     FAHGKFRPLDDTPPEWRTLHHLGAGNFVVPGAQIQGERLQ"
     gene            7056..8090
                     /locus_tag="PKV30_03535"
     CDS             7056..8090
                     /locus_tag="PKV30_03535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRL29326.1"
                     /translation="MTKALKDIAFSMLDLVPVREGRSVADALAVSLQTARHAERLGFT
                     RYWLAEHHNMAGIASSATAVLVGHIAGGTERIRVGSGGVMLPNHPPLTVAESFGTLAE
                     LYPGRIDLGLGRAPGTDPMTMRALRRNRVETVDDFPREVAELEHLLGDVTPGQKLIAN
                     PGAGTRVPIWLLGSSLFSAQLAAERGLPYAFASHFAPAMLLQAIDVYRRAFKPSAQCP
                     KPYVMIGVPLVAAPTDEEATYLAGTIFQRVLAILRGARGLLQPPRPGFFEQLNAQEKA
                     GIADFLACAVIGSPETVRQGLQTLADETGCDEMMFTCDIYDPALRLRSLDIAAAVRAG
                     DSAPVREPAA"
     gene            8087..8389
                     /locus_tag="PKV30_03540"
     CDS             8087..8389
                     /locus_tag="PKV30_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29327.1"
                     /translation="MTRARVGLGALVVLALLAQGCAVASATAGAAVAVTGAVVATGVS
                     LTGKAIGAGIDAVSGPAESPDRSGIVVRERIRPAEAASPPRCAPTAGDAPAPADCR"
     gene            8467..9087
                     /locus_tag="PKV30_03545"
     CDS             8467..9087
                     /locus_tag="PKV30_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HRL29328.1"
                     /translation="MHELPHSPAAERNQAPILAVLHTLLPASGRALEVASGTGQHAAH
                     FAAALPGWQWQPTDLDDAGFDAIRGWAQRAGARNVEAPRRLNAQDAVWPSDGTPFSKP
                     FDLIYAANLLHISPWATSRGLMQGAARHLAPTGQLVVYGPFIETDVTTAASNLAFDAD
                     LRRRDASWGLRRLDDVVAEAHAAGLRLAERHALPANNLLLVFAHGS"
     gene            9149..9769
                     /gene="gmk"
                     /locus_tag="PKV30_03550"
     CDS             9149..9769
                     /gene="gmk"
                     /locus_tag="PKV30_03550"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRL29329.1"
                     /translation="MDHPGNLFVVAAPSGSGKSSLVNALLEVDAGVAPSISHTTRAPR
                     GQEKDGREYFFIDDQTFDAKIAAGDFLEWALVHGNRYGTSRQAIEARMQAGRDVVLEI
                     DWQGALQIQKLFPDAILVFVLPPSWDELRARLTRRGEDAPEVIELRLANAREELAQCQ
                     KFDFVIINEVFERALFDLKAIVHAQRLKYSAQKSARAGTFEGLGIA"
     gene            9801..10001
                     /gene="rpoZ"
                     /locus_tag="PKV30_03555"
     CDS             9801..10001
                     /gene="rpoZ"
                     /locus_tag="PKV30_03555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRL29330.1"
                     /translation="MARITVEDCLDQIPNRFQLVLAATYRARMLSQGHTPKIETRNKP
                     GVTALREIAAGKVGIEMLKKVP"
     gene            10109..>11996
                     /locus_tag="PKV30_03560"
     CDS             10109..>11996
                     /locus_tag="PKV30_03560"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRL29331.1"
                     /translation="MPIVERPMAAANAAAASFAALEAKLDYLDAADIDLVRRAYRFAD
                     TAHLGQMRHSGEPYITHPIAVAAQVAEWKLDAHALMAALLHDAMEDCGVTKADLVEQF
                     GAPVAELVDGLTKLDKLQFSTREEGQAESFRKMLLAMARDVRVILVKLADRLHNMRTM
                     GDMPRPKWGRISTETLDIYAPIAHRLGLNETYRELQDLAFRHLQPWRYSVLAKAVHKA
                     RQRRRDLIQKVQHDVTAALADAGLNAHIVGREKTLYSIYRKMDEKHLSFAQVNDLYGV
                     RVILPNIISCYTALGMLHQLYKPVPGRFKDYIAIPKINGYQSLHTTLVGPASVNIEFQ
                     IRTEAMDLVAESGVAAHWLYKAGQRWEDKTSPEELGTRWLQSLLDIQNETRDATEFWE
                     HIRVDLVPDEVYVFTPKGQILSLPQGATVVDFAYAIHSNVGDHAVGAKVNGEQVPLRS
                     ELANGDVIEIITAPVSTPNPAWLGFVRTGRARSKIRSHLKSLAQDESRELGEKLLAQA
                     LRAEGLGQPLGQQTADKPLWDKLLRFTGNRTREEMMTDIGLGKRSANMVAKRLVTLLA
                     AEGVKPDALLLTHARYTAHENLSQGAVILDGAENISVKYATCCRPIPGDGVLGYLGRG
                     EGLVV"
BASE COUNT         1933 a         4211 c         4019 g         1833 t
ORIGIN      
        1 aacagcacgt tgatgaaggc gttgatgaag cgctcggtgt cctgccgcac gcgcagcagc
       61 agcgacagcg tttcgccgct gcgcgcgtct tcgtattcct gaaaattcag gcgcatggtt
      121 tgccgcaaac cgtcgttgaa gatctgcgtg ccaaagcgct gcaccaccat gcgcacggcg
      181 tagtcctgca aggcgcgtgt ggcgcgcgag gccaatgcca cgcccacggc cagcgccagc
      241 aacatcagcg cgccgcgcac cagttcgtcg tcgggcttgg cgcctttttg cgtggcgtag
      301 ccgtcgatca gcttgccgaa gatgatgggg tcgatcagcg ccagcacctg gctggccgcc
      361 gccagcccca tggccaccag cgccagccgc cattgcggct tgaggtattt ccagagcgtg
      421 tgcatcggca cgcatcatga ccgccctggc gcgcgccgtc cgtcggacgc gtgcgccttc
      481 agcgccgccg acggacgaca ccgaaaacta ctgaatcgat agcgcctcgc gcttgtcccg
      541 cttgcgccag tggcctattt cttgtcgacg gccggatcca gcacgcgaag cagccagcgc
      601 tgcacgtcct gcacttccgg tgcgcagacc gaatggccca tcgggtactg gtgccattcg
      661 acggcgtagc ccagcgccgc cagcgcatcg cgcgaggcgg cggcgcgcgg cagcgggatc
      721 atctcgtcct gcgtgccgtg cgccaggaag atcggcgcgt cggcattggc gctgctgcgc
      781 tcggccgcca gcgcgccggc cagcggcagg tagccgctca tgcccaggat gccggccagc
      841 cgctgggggt agcgcagccc cgtcagcaac gccatggcgc agccttgcga gaaaccgccc
      901 agcacgatgc ggtgcgccgg cacgccgcgc gcctgctcgc gcgccagcag cgcgtcgatc
      961 gacgcctgcg agcggcgcag tccggcctcg tcttcgcgac tgcggtcggc ggcaatgtcg
     1021 taccacgccg gcatgacgta gccgccgttg atgctgacgg ggatttgcgg cgcgttgggg
     1081 aacagccagc gcacgggtcc gatggcggcc aggtcgatct cgtcgacaaa gggcgcgaag
     1141 tcgctgccgt cggcgcccag gccgtgcatg acgatgatgc tggcgcgcgg gttggcgccg
     1201 gtttccattt caagggtggg gggtggcgag ctcatgccgc atcgtaaccg tgcgtcggca
     1261 acgctgctgg attgcaggaa aaatacggcc ccagcgcttg ccagtcaagc gcggacagct
     1321 atcaaaacta aagtacctgg aatgcggggc gctcaggcca gcgccggctc gcgctcggcg
     1381 cgcgcggcct cgcccagctg gcgcagcgcc ttgtcaacca ccgcgccggc atcgaccgga
     1441 cgcaactggc gctcgcggat cagcttctcg caggtgcgcc gtgtcatgct gtgcggacgc
     1501 ccgccgcggc tgatgaacat gaacagcgtg ccgttctcgc tggtccagtg caattgcgcg
     1561 cgccgccagt ggccgcgcac cttcagatcg acccagtcgc cggtgcgcag gcgtgccagc
     1621 tgggcgcgcg tgcgggcttc gatgtcttct ggcgtttcgg ccgccttggc cgcggctgcg
     1681 cgtggggccg cggccggcgg cttcaccaca tccgccatgg acaagtccag tggcacgaaa
     1741 ccttcaacct gcgcagggac gtgcgttggc tcttgggccg gctccagcgg ttcggtagag
     1801 gcaaagtcgt cgttgtccga gggttgcacg gactcgtctt cgagcggcgc gctctcggtc
     1861 agcggcgcct gcgcagattc gccctcgctc agctcctcga agccggcggc cgccagttcg
     1921 tggcggccca gccacggttg ctcggccgcg cgcggctgcg gtctttccag cggtgccgga
     1981 tcggtctcca gcggcaaccg cgcagattcg tccgccagcc gcgaatagcc cgaggcctca
     2041 gcgtcgcgcg cgctgcgcaa ccggcgcagg cgcagcaccg ggtcgtggtg gcgcatcagc
     2101 gcgtcgaaga aggtttcggt ctcgtgcggc tccttgccca gcatctgcag accgcgccgc
     2161 agcgtctgca ccagaccggg caccacttcg aacagacgag ccggcgcttt cagtgcggcc
     2221 tcgcgcttga cgctccacag caaatcggtg acgacggcca gatagccgcc cgggtcgagc
     2281 tggccgcgct gatcggtgag ttgcgcgtgg gcaatcacca gcgaccagtc cttgaacagg
     2341 aagtcggcca ccacagccgg cacgcggtcc aggtcggagc gcaggctgaa ttcccacgcc
     2401 accttgtcgg ccagcgcctg gcgctgctgc gcgaaatgca tcgagcgcag gccgtcctcg
     2461 cgcgcgcgct cgtccgaagt gtcctgctgc tgccagtcgg cttccagcga ctgcaagcgc
     2521 gcattgaagt tgtctgcgcg cgcttcggtt tgctggttga gttcgcgcac cgcctggcgc
     2581 accggcgcca tgaactgctc gaactcgggc gcgaactcgt cgttgtactt gaagctgcgc
     2641 tgcgccacgc cctcgatcag gcggcgcgcg gggtggtggt cgtcgccgaa gaagcgcgga
     2701 tcgtgcatgg ccatgcgcag cagcgccggt tccatggcga caaaggcctc gcgcaccggc
     2761 gccagcacgc gctggtcgcc cgcgacctgg ccgaccagcg tgcgcaccgc gtccagaccc
     2821 agcacctggc tgatcttgcc ggcctgcgcc ttcagctgca gcagtgtctg cgcccgcgcc
     2881 tgcggcgacg cggcctcgcc ccactgctcg ggcgccaacg gagtatcggg cgccacctcg
     2941 cgcggcgcgc ggtcgaccac gctttgcgcc tgcgcacgcg cgcgggtgcg ggccgcggtc
     3001 gcagcgtcgt actggggcgc gggcgcctgc gccacgcgct gtgcctgcgc atcgacggct
     3061 tcgtagtagg acgccggcag cggctcgtcg ttttcggcca gccactgagg tcggtacaga
     3121 aactcgtgca tcagcgcgcg cggcacggcc ggctgcgcgt gcgccaggtc gctcatgcgc
     3181 ggaaacagcg cgcgccgctg gcgcacggcg tcggcatcgg acgtcatggc accgccacca
     3241 cccgcgccac tacccgcgcc gccgccagcg ccagcgcccg aaccggaacc gcccgccgcg
     3301 tcgtcgacct tggcgctgcc gcgcggcaag gcaccccctt cggtcagctt caggcggtag
     3361 cgcgcttcct cgacgccttg gtcctcaagc aactgggcga cggattcgta gaggcggttg
     3421 agctcgcccg caaagggctt ggccatgtgg cgcgcccaat gcgcacgcag ttccgggtct
     3481 tccggttgat ggcccagcaa ttgcagcagc gcgccgcaca gcacgtccgg gcgcagcggg
     3541 ttcagctcgg cccgcaccac cggcaggccg agcgccgaac tcatgagcga atcaagtcgc
     3601 gacaggtttt gctcgaccac cggcatcacc gtctgctgca ggcgcgaagc ttcgacaaag
     3661 cgcgccacct cggcgtcttc gaccaaggcc aggtcgtcat cggccactgg tgcgcgcgcg
     3721 ccgctcgccg gcacctcgcg cgcgctcatc agtgcctcgc ccaccgcctg gtccacctgg
     3781 cccgggaagg cggcgcgcaa ctcggcccgc cgcttggtca gcgccgccat cgcttcgccc
     3841 agctgcaacc tgagcgcgac ggatttggca ctgcgctcgg cgccctccag cgccgccagc
     3901 gcggcttcca cgcagcggtc gatcagcgac gacgacagga agaccgcttc ctgccgggcg
     3961 cgcttgatca aggggctttg ggtgctgacg gcttgcatga acaggaaaga attacctccg
     4021 ccgattcgag cgaacggggc acggcgagat gggcatctgc cagactttat agcgtgtcgt
     4081 gcgcgccgaa gccatgacag agcccgtcag ggggctgaaa agagccaact atgtcaactt
     4141 tggcgaactg tggcgtcggc atcgaatatc ggatcgaccg gcacctgcag cgccgtcacc
     4201 aggtggttgg tcttgcccgc caggccgacg atggccagca actcggcgta ctgcgccgcg
     4261 ctcatgccct tggcgcgcgc tgcggcgctg tgcgagtgca cgcaatagcc gcagccgttg
     4321 gccaccgaca ccgcggtgta aatcatctcc ttggtcagcg gatcgagcgc cgagggcgtg
     4381 gccatcacgc gcttgacctc ggcccaggtg gcttccagca ggtcggcgtc aaaggccagg
     4441 tagcgccaga agttgttgat gaagtccgaa ccccgcgtgg tgcggatgtc gtcgaagaca
     4501 gcgcgtacgc gggcgtccgg gtgcgcgtca tcggcggggg cgacggtgga catcgattgc
     4561 tccaatgcta ttgtttacat agctgtccgc gcttgtcaat caagcgctgg cggcattttt
     4621 catgctgaaa cccggatcgg gcgcgtcagg tacacagctt cgcgcccgac gatcggctcg
     4681 cgcgcgggca tcagcaccgt gcaatcgtcg cacggcgcgc ggatctcgcc ggcgtcgccg
     4741 tcggtggcga tcaactcgcc ctcggcgaag gtctcgaagc cgatcaacgg tcgggcaaac
     4801 ttgaaatcgg gcgtgtgcac catgcaggtc tgcagcagct cgaagcgccg ctgcggcgcg
     4861 gccgacacgc cgcgcggcgc tggctcgatc agcccgaagt gcgccagaaa gtccagcgcc
     4921 gtcgccgtcg ccagctcggc gctggcgcgc agaaaatgct gcccgcactc caccaccagc
     4981 gccacgccgg cggcgtcgcc atccaggcca tgccgaccgt gctggatcag cggcgtgccg
     5041 ctgcccaggc cgctgggcat gaccaggtgg gtgccgacgc gccccagcgc ccccagcgcc
     5101 agcgccgcgc gggcattgcg cgcaaagttc ggataaaccc agaacggcgc caccggctgg
     5161 ctggtggagt gaatgtccaa aatgtggtcg gccgctgcca ccaccggccg cagcgcccgc
     5221 gcacggcgca actcggggcc gttctgcggg ccgtccagcc actcgtccga ccagatgcgg
     5281 ttgaggttgt gcaccagttg ccggttggca aacggcgcgt cgcgctgaaa cgcctcgtac
     5341 gcctcgacgt gcgcgaagct caccgtcagc gtgccgatgc gcgggcgcac gccgttgtcg
     5401 agcaggtgcg tggccgcgac catgccgcag atttcgttgc cgtgcgtcag cgcgttgatc
     5461 agcacgtgcg gcccggcggt gccgctgtcg aagcggtgca cgtagtcgat gccggtgttg
     5521 ccggcgcgat aggccgacaa atcgcgcggt acaacttcaa gagcgggagt ggaatgatcc
     5581 atgcggtggt caggcaacga cgcgaaagac gccgatctta tcggcccggc taagctcgcg
     5641 cgcatgacca acgccctgcc cgcccacgtc caccccgttg aactcaagca cgcctaccgc
     5701 ctgctcaaca ccggcgcctc gatcctcatc agcagcgcgc atggcggccg cggtgacgtc
     5761 atggcctgcg cctggaacat ggcgctcgac ttcacgcccg ccaagctggc ggtgtgcctt
     5821 gacaaggcga cgctgacgcg cacgctggtc gaggccagtg gcgagttcgc catcggcgtg
     5881 ccctgcgcgg cgcaggccga tctggtgttc agtgtcggca gcgtcagcgg gcatgatctg
     5941 cgcgagaaag acaaattcag cgctttcaac atcgatcatt tcagcgccag ccgcgtcagc
     6001 gcgcccctgg tctccggctg catcggctgg ctcgaatgcc ggctgatccc cgagccgcac
     6061 atccagcagg cgcatgacct gttcgtggcc gaagcggtcg ccgcctgggc cgacgagcgc
     6121 gccttcgccc acggcaagtt ccgcccgctc gatgacacgc cacccgaatg gcgcaccctg
     6181 caccacctgg gcgcgggcaa cttcgtggtg ccgggggcgc agatccaggg cgagcgcttg
     6241 cagtgacccg cactggcacg gccagcgcaa ggcgtcggat cagggctcca gcgttgcctt
     6301 gagcagcgcg ccatcggcca ggatgtagag cgagccgtct gcaccggcag ccagcgcctg
     6361 tactgcgccc aggtgggcgg gtagtgcacc ggttcattgc cgcgataggg cagccgcgga
     6421 cgacgcgggt cggtcggggc gaccgggaag tcgttgatgc tgcacgcggg gtcgcgcggt
     6481 gcgggctcgg tgcccacgac cggcgtgcgc gtgccgtcgc gcgccagctt gtaaaccacc
     6541 gccccgcgcg ccacgtacag cgcgcggcgg tgtggggcag tgctcggcgt tgggccctgc
     6601 ccactcagcc atcaccacgc tgccgccagg cgcttcggtc atcgacaaga cgctgcgaaa
     6661 acgcacttcg gcgcccacac cgtcgggcgc atcgaagcag ccggggccga actcggatgg
     6721 cagcaaaggg ctggccatca gcgtcaattg gcctcccgcc gccggctgcg cgggcgtggg
     6781 tgcaggcagc ggcacaggcg cgggggccgg tgctggcgca ggcgatggca cgggtgtggc
     6841 cgggctgctc ccgtcgccac cgcaggcgca agcggccggc gcagtggcga aaaaacagca
     6901 tggtcaagcg cggcaatagc cgatggggca ggctcatgtc gtgtatctcc aggagcggtc
     6961 ggtcaagggt ggttctgacg cgctcggcgc caatgccatc gcggtcaaac ccgggctcgt
     7021 cacgcaggca ccccgaatcc gcacaaaata gccccatgac taaagccctc aaagacatcg
     7081 ccttctccat gctcgacctg gtgcccgtac gcgaggggcg cagcgtggcc gacgcgctgg
     7141 cggtttcgct gcagaccgcg cggcacgccg agcgcctggg tttcacgcgc tactggctgg
     7201 ccgagcacca caacatggcg ggcatcgcca gctcggccac cgccgtgctg gtagggcaca
     7261 tcgcgggcgg caccgagcgc atccgcgttg gctcgggcgg cgtgatgctg cccaaccacc
     7321 cgccgctgac ggtggccgag agcttcggca cgctggccga gctgtacccc ggccgcatcg
     7381 acttggggct gggccgcgcg cccggcaccg acccgatgac catgcgcgca ctgcgccgca
     7441 accgcgtcga gacggtggac gactttcccc gcgaagtggc cgagctggaa cacctgctgg
     7501 gcgatgtcac gccgggtcag aagctgattg ccaaccccgg cgccggcacg cgggtgccga
     7561 tctggctgct gggctccagc ctgttcagcg cccagctggc ggccgagcgc ggcctgcctt
     7621 atgcctttgc gtcgcacttc gcgccggcca tgctgctgca ggccatcgat gtgtatcggc
     7681 gcgccttcaa gccctcggcg cagtgcccca agccctacgt gatgatcggc gtgccgctgg
     7741 tggcggcgcc gacggacgaa gaagcgactt atctggccgg caccatcttc cagcgcgtgc
     7801 tggcgattct gcgcggcgcc cgcggtctgc tgcagccgcc acggccgggc tttttcgagc
     7861 aattgaatgc gcaggagaag gccggcatcg ccgacttcct ggcctgtgcc gtgatcggct
     7921 cgcccgagac ggtgcgccag ggcctgcaaa ccctggcgga tgagaccggc tgcgacgaga
     7981 tgatgttcac ctgcgacatc tacgacccgg cgctgcgact gcgctcgctc gatattgcgg
     8041 ccgcggtgcg cgcaggcgac agcgccccgg tgcgcgagcc ggccgcatga cgcgtgcccg
     8101 cgttggactg ggcgcgctgg tcgtactggc gctgctggcc caaggctgcg cggtggccag
     8161 tgccacggcg ggcgcggcgg tggcggtcac tggcgcggtg gtggccaccg gcgtcagcct
     8221 gacgggcaag gcgatcgggg caggcatcga cgcggtgtcc ggcccggccg aatcgcccga
     8281 ccgcagcggc atcgtggtgc gcgagcgcat tcgcccggct gaagcggcgt cgcccccacg
     8341 ctgcgcgcca accgcgggtg acgcacccgc gcccgcggac tgccgttgat gccgaattag
     8401 ctattcttta aatagctgct cgcgctttcc ccgcaagcgc cagcggcctg aaacacttgg
     8461 aacccgatgc acgagctgcc tcacagcccg gccgccgagc gcaaccaggc gcccatcctg
     8521 gccgtattgc acactctgct gccggcctcg ggccgcgcgc tcgaagtcgc cagcggcacc
     8581 ggccagcacg cggcccattt cgctgccgcc ctgcccggct ggcaatggca gccgaccgac
     8641 ctggacgatg ccgggttcga cgccatccgt ggctgggcgc agcgggcagg cgcgcgaaac
     8701 gtcgaggcgc cgcgccgact caatgcgcag gatgctgtct ggccgtccga tggcacgccg
     8761 ttctccaagc cgttcgatct catctacgcc gccaacctgc tgcacatcag cccctgggcg
     8821 acgagccgcg gactgatgca gggcgcggca cgccacctgg cgccgaccgg ccagttggtg
     8881 gtgtacgggc cgttcatcga aaccgacgtg accacggccg ccagcaacct cgccttcgac
     8941 gccgacctgc ggcggcgcga cgcgtcctgg ggcctgcgcc ggctggacga tgtggtggcc
     9001 gaggcgcacg cggccggttt gcggctggcc gagcgccatg cgctgccggc caacaacctg
     9061 ctgctggtgt tcgcccatgg cagctaaaat tcgccgcacc gtcgttcacg gtcactcccg
     9121 ttttcatagc tgctcgcgct tgtcccgcat ggatcatccc ggaaatctct ttgtcgtcgc
     9181 ggcgcccagc ggttcgggca agtcgagcct cgtcaacgcc ttgctggaag tggatgcggg
     9241 ggtggcgccg tccatctcgc acaccacgcg cgcgccgcgc gggcaggaaa aagatggccg
     9301 cgaatacttt ttcatcgacg accagacctt cgacgccaag atcgccgccg gcgattttct
     9361 cgagtgggcg ctggtgcacg gcaaccgcta cggcacatcg cgccaggcga tcgaggcgcg
     9421 catgcaggcc gggcgcgacg tggtgctcga aatcgactgg caaggcgcgc tgcagatcca
     9481 gaagctgttt cccgacgcca tcctcgtctt cgtgctgccg ccgagctggg acgagttgcg
     9541 cgcccgcctg acgcgccgcg gtgaggacgc gcccgaggtg atcgagctgc gcctggccaa
     9601 tgcgcgcgag gaactggcgc agtgccagaa attcgacttc gttataatca acgaggtatt
     9661 tgaacgcgcg cttttcgacc tgaaagccat tgttcacgcg caacggctga agtacagcgc
     9721 ccagaaaagc gcccgcgccg gcacctttga aggcctcggc atcgcctgac acccattcac
     9781 ctccaggaca ctcccgcacc atggcccgca tcaccgtcga agactgcctc gatcagattc
     9841 ccaaccgttt tcagctggtg ctggccgcca cttaccgcgc ccgcatgctg agccagggcc
     9901 acacgcccaa gatcgaaacg cgcaacaagc ccggcgtgac cgcgctgcgc gaaattgccg
     9961 ccggcaaggt cggcatcgag atgctgaaaa aagtgccctg aagcggcagc cgcttcaagc
    10021 ccgcaaaaag cgcccatcgg gcgctttttt gttgcgtttg cacaatttcg tgtcaccggg
    10081 gttaaattaa ggccttatgg gatccctcat gcccatcgtc gagcgcccga tggccgccgc
    10141 gaatgcggct gcggccagct ttgccgcgct cgaggccaag ctggactatc tggacgcggc
    10201 cgacatcgac ctggtgcgcc gcgcctaccg ctttgccgac accgcccacc tgggccagat
    10261 gcgccacagc ggcgagccct acatcaccca ccccatcgcc gtggccgcgc aagtggccga
    10321 atggaagctg gacgcgcacg ccctgatggc cgcgctgctg cacgacgcga tggaagactg
    10381 cggcgtcacc aaggccgacc tggtggagca gtttggcgcc ccggtggccg aactggtgga
    10441 cggcctcacc aagctggaca agctgcagtt cagcacgcgc gaggaaggcc aggccgagtc
    10501 cttccgcaag atgctgctgg ccatggcgcg cgacgtgcgc gtgattttgg tcaagctggc
    10561 cgaccgcctg cacaacatgc gcaccatggg cgacatgccg cgccccaaat ggggccgcat
    10621 cagcaccgag acgctcgaca tctacgcgcc catcgcccac cgcttggggc tgaacgaaac
    10681 ctaccgcgag ctgcaagacc tggcctttcg tcacctgcag ccgtggcgct acagcgtgct
    10741 ggccaaggcg gtgcacaagg cgcgccagcg ccggcgcgac ctgatccaga aagtgcagca
    10801 cgacgtgacc gcggcgctgg ccgacgcggg gctgaacgcc cacatcgtcg ggcgcgagaa
    10861 aacgctttac tccatctacc gcaagatgga cgaaaagcac ctgtccttcg cccaggtgaa
    10921 cgacttgtac ggcgtgcgcg tgatcctgcc caacatcatc agctgctaca cggcgctggg
    10981 catgctgcac cagctctaca agccggtgcc ggggcgcttc aaggactaca tcgccatccc
    11041 caagatcaac ggctaccagt cgctgcacac cacgctggtc gggccggcca gcgtcaacat
    11101 cgagttccag atccgcaccg aggcgatgga cctggtggcc gaatccggcg tggccgcgca
    11161 ctggctctac aaggccgggc agcgctggga agacaagacg tcgcctgaag aactcggcac
    11221 gcgctggctg cagtcgctgc tcgacatcca gaacgaaacg cgcgacgcca ccgagttctg
    11281 ggagcacatc cgcgtcgacc tggtgcccga cgaggtgtac gtcttcacgc ccaagggcca
    11341 gatcctctcg ctgccgcagg gcgccacggt ggtcgacttt gcctacgcca tccacagcaa
    11401 cgtgggcgac cacgccgtcg gcgccaaggt caatggtgag caggtgccgc tgcgcagcga
    11461 actggccaac ggcgacgtca tcgagatcat cacagcgccc gtctccacgc ccaacccggc
    11521 gtggctcggc tttgtgcgca ccggccgcgc gcgcagcaag attcgcagcc atctgaaaag
    11581 cctggcgcag gacgaatcgc gcgaattggg cgaaaagctg ctggcgcagg cgctgcgcgc
    11641 cgagggcctg gggcagccgc tcggccagca gaccgccgac aagccgctgt gggacaagct
    11701 gctgcgcttt accggcaacc gcacgcgcga agagatgatg accgacatag gtctcggcaa
    11761 gcgcagcgcg aacatggtcg ccaagcgcct ggtcacgctg ctggcggccg aaggcgtgaa
    11821 gcccgacgcg ctgctgctga cccacgcgcg ctacaccgcg cacgagaacc tgtcgcaagg
    11881 cgccgtcatt ctggatggcg ccgagaacat ctcggtcaag tacgccacct gctgccgccc
    11941 gatccccggc gacggcgtgc tgggctacct gggccgcggc gaaggcctgg tggtgc
//
LOCUS       DAOMVO010000040        11669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1299_length_11669_cov_16.592905, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000040 DAOMVO010000000
VERSION     DAOMVO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1299_length_11669_cov_16.592905"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKV30_03565"
     CDS             <1..837
                     /locus_tag="PKV30_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HRL29332.1"
                     /translation="RAVQAEGLRDDWYNTTSADPQSPVAMGGVQPDTWRNIGGLRNAV
                     SILHETRGIGLGKAHLARRVQSHVVAAQALIEQMAAQGPALVALGRRVDRDVAAQACK
                     GQITVAAGQTPEKRTLTFYDAKTGAELKPTVDWGSALTLRVQRERARPCGYWLAPSEK
                     AAVEALRALGVQVKSMPGARQVSAERYVFKSAEQGQRQDGRGAIQDAGGIVKTEVALA
                     PARVRLPAGGWTVSLAQPLAGLVTAALEPDSQNSYVAARMIAVPKEGEGDRLLRLMAP
                     VR"
     gene            complement(955..1599)
                     /locus_tag="PKV30_03570"
     CDS             complement(955..1599)
                     /locus_tag="PKV30_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29333.1"
                     /translation="MAEDALALARCRFDGWDAAGAPEVWALPQPDRAQAHPEDERAHQ
                     RQAARDALRARLAAVFGVATEDIAISQQRGAAPRVSAHVGDSTRWANVGLSIGHERGV
                     SLIAWHGAGLVGVDVVRLAVHTDAAELARVAQLYLEPNRPSALVHQAQTAINTGAFLS
                     HWACHEARLKCAGLALVEWSDALATQLAGIHAVPVKLPPWAGDAAGAVAWRWRY"
     gene            complement(1608..2825)
                     /locus_tag="PKV30_03575"
     CDS             complement(1608..2825)
                     /locus_tag="PKV30_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HRL29334.1"
                     /translation="MTPRFHPSALALALLVGGASAQTAPKQAEVAQRVEKAAAWQQCT
                     GMANNERLACFDDWAREQRELVTAIEDKARSVQASPNAQASGVRAEVAAALASTQPES
                     APGTTPSGSAGIIGVGLEQGCRDRQFSELSRFWELESGSSCPTFGLRGFRPNTVAIAG
                     SDEVNRQPTSPNPINSATESIDYSKQAMRLHLSVRTKLASGLLTPKDGTLRDSLWAAY
                     SQQSEWQAFNSRLSRPFRNTDHMPELIYVYPTTLQLPGGWLWRYSGVGLAHQSNGQSD
                     PLSRSWNRAYVMAGFEKDNRFNVQMRLWKRLSESAAKDNNPDLTRYLGRGDVTVGWNI
                     DSKNSLRATYTGFNKRGSGRIEWTRSLGDGLGNSFSGLRLYTALFHGYGDSLLDYNFK
                     RTVFSIGLSLVDF"
     assembly_gap    3037..3136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3137..6241)
                     /locus_tag="PKV30_03580"
     CDS             complement(<3137..6241)
                     /locus_tag="PKV30_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRL29335.1"
                     /translation="MKPPPRACRKTALIFGERHLTYAELNDAADRVAHRLIEAGVRAG
                     DMVGLWHPRGIELLVLQLGIAKTGAAWLPFDADVPTDRIAVCLDDASAKALLISELSA
                     QAVQAQSGISAQILTASELLSALPEGTALRRREGALPEHTAYVIYTSGSTGKPKGIAI
                     TQGSISHFLRSENARLGVREDDKVYQGFSVAFDMSFEEIWISYLVGATLWIAPKEIAG
                     DPEALPRALIENDVTVLHAVPTLLALFAQDVPGLRIINLGGEMCPQALVDKWATPARQ
                     MFNTYGPTEATVSASLAELKAGEPVTIGQALPNYGLLVIEVIDPDSIVNGMVPPLKIL
                     PFGDTGELCITGPGVAAGYLGRPDLTAEKFLPNPWARNEQEARLYRTGDLARVENGTQ
                     MQCLGRADDQVKIRGFRVELGEIEAVLAEQPGVGTTAVLLRKEGDIDQLVAFYVPSGA
                     TPPAIKDLRAGLAEKLPPYMVPARFEALPAMPRLQSGKIDRKVLKSQPLSAAAPAEGS
                     DTPETPAEEVLFAALEKLFPGQPIRRELDFFSDLGGHSLFAARITSTLRQDPRFAQAT
                     VADIYQNRKLGLIAEALQAGMVGAEATADRPFVLHSAWRRWRCGIAQGLAIPFLVLLT
                     MAQWLAPFFAYHFNTGDADDKISQAVFASVLTFIVAIVLNFAIALFARRVIAGNLKPG
                     SYPLWGLTYYRWWLADRISEAVPAYMINGSPLYAAWLRLQGAKVGPEVNLGSVTIRVP
                     HLVRIGEGASIGNVVNLENARVEGGLLHLGTIDIGAQANVGSYVVIEGDTRIGEYGHL
                     EGQSALASGQQVPARKVWHGSPARERGDFDPATLPARPAVTTARLWWERAVFITGAVL
                     IAVLFFMPVFPTFLLIDWLDVPNLSVRPLVDDGSITALQAFGLRLVKFFLLALPASGV
                     FIFATVLLSAFIRWALLPKMKAGSWPIHSNRYLAKWLVSQIQESSLAVLHGIYATIYS
                     AAWYRLLGAKVGKETELSTALGVVPDMLTLGDQCFIADAVMLGDEHIDGGWMTVQPTV
                     VSRRS"
     gene            6417..6866
                     /locus_tag="PKV30_03585"
     CDS             6417..6866
                     /locus_tag="PKV30_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HRL29336.1"
                     /translation="MCAAPPLLLNLQGARRRLLYVTLFEGLAIAIVTLALLVFTGAGA
                     ASAGGLAIGSSAIALGWNLMFNMLFERWERRQPTRGRSAARRVAHAIGFEGGLLLFLV
                     PFFAWWLGVSLWQALVLDIGFLLFFLVYNFVFAWAFDRLFGLPAGVG"
     gene            6971..8191
                     /locus_tag="PKV30_03590"
     CDS             6971..8191
                     /locus_tag="PKV30_03590"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRL29337.1"
                     /translation="MLQLADIQAAAARLQGQVLDTPCVESRTLSQVTGAQVFLKMENL
                     QFTASFKERGACNKLVQLSAEERARGVVAMSAGNHAQGVAFHAQRLGIRAVIVMPRFT
                     PGVKVARTRGFGAEVILHGDTLDAASEHARTLAREQGLTFVHPFDDEAIMAGQGTLAL
                     EMLAEQPDLDTLVVPIGGGGLISGVATAAKALKAGIRVVGVQTARFPGAVNAITGSAH
                     AQGASTIAEGIAVSSPGAQCLPLIRQHVDELLLVDEGDIEQAIVMLLEIEKTLSEGAG
                     AAALAALLRYPEQFKGRKVGLVLSGGNIDPMLLAAIIGRGMVRSGRLARIVVSARDVP
                     GSLARVTTLVAEAGANVQEIHHQRAFTLLAAQNVEIEMVLQTRDTAHVQELLARLAAE
                     GLEARWLGAARVSD"
     gene            complement(8348..8848)
                     /locus_tag="PKV30_03595"
     CDS             complement(8348..8848)
                     /locus_tag="PKV30_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRL29338.1"
                     /translation="MPDLDPTALSQRLHQAGLRSTRAVLGVAELFDGAAAGWAPTHAE
                     VADQLAQSGAPMNAVTLYRLLDRLVAAGLLARHTAPSERTWRFRWLGMGMGIDERAST
                     AAVPHFECDACHTHFPLQDADAPTQAVADALRRTLAEYGHQAARVDLAVHGTCAECRG
                     DAQPYP"
     gene            8939..9064
                     /locus_tag="PKV30_03600"
     CDS             8939..9064
                     /locus_tag="PKV30_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29339.1"
                     /translation="MTPPPSLPKRPPLLLRAAGWRLLVAAGFSALLLLLWRWAVI"
     gene            9066..9878
                     /locus_tag="PKV30_03605"
     CDS             9066..9878
                     /locus_tag="PKV30_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29340.1"
                     /translation="MLAPQPITLRDLTLGYDGHPAVHHLSGTIAPGALLAVVGPNGAG
                     KSTLIKALAGLLTPIGGHIDGLAGQRVAYLPQQAALERGFPIGVGEFAAMGLWHETGA
                     FGGFSRAQRERVRAALAAVGLEGHARQPIDTLSGGQLQRALFARLMLQDAPVLLLDEP
                     FSAIDQRTTVDLLQLLRQWHAAGKTVLAVLHDLHQVRDAFPETLMLARELVAWGRTDE
                     VLTPANLQRLRAMPEAFDDRAAVCEVPAAGTPHDHGHAHDHQHHHGATEPRS"
     gene            9875..10771
                     /locus_tag="PKV30_03610"
     CDS             9875..10771
                     /locus_tag="PKV30_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRL29341.1"
                     /translation="MTPEIADTGLWALLIAPLTEFGFMRRALVGCVALSLSCAPVGVF
                     LLLRRMSLTGDAMAHAILPGAAIGYLLAGLSLPAMTLGGIVAGLLVAIGSGLVARSTV
                     LREDASLAAFYLLSLALGVLIISTRGSSVDLLHVLFGTVLALGDGALGLLVGIAITTM
                     AALALMYRPLVLECLDPQFLRCVSPASPVAHYGFLVLLVINLVAGFHALGTLMAVGIM
                     VLPAATARLWVRSVPALMALAAALALAAGVAGLLISYHADWPTSPTIVLALGAAYLVS
                     LVVGRYGAWRGGQHGRRRHLRA"
     gene            10999..>11669
                     /locus_tag="PKV30_03615"
     CDS             10999..>11669
                     /locus_tag="PKV30_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRL29342.1"
                     /translation="MSLLIPRRIALIAALASAMAAPAHTAEPLRVVATFSILGDLVQQ
                     VGGQRVAVQTLVGLGGDAHVFQPTPTHARQVGQAQLVVSNGLGYEGWMPRLLQSTGYK
                     GLQVIATKGVRPIEGGKDHDHDHDHDRADPHAWQSVPNAIVYVANIADGLCAADAAGC
                     ADYRTGAARTTDALKKLDAGIRAAWAPIAPAERKVITSHAAYAYYGQAYGVRFLSPQG
                     VSTES"
BASE COUNT         1747 a         3827 c         4084 g         1911 t
ORIGIN      
        1 cgcgccgtgc aggccgaggg gctgcgcgac gactggtaca acacgacctc ggccgacccc
       61 cagtcgcccg tcgccatggg cggcgtgcag ccggacacct ggcgcaacat tggcggcctg
      121 cgcaacgcgg tcagcatcct gcacgagacg cgcggcatcg gcctgggcaa ggcgcacctg
      181 gcgcgccgcg tgcaatcgca tgtggtggcg gcgcaggcgt tgatcgagca aatggccgcg
      241 caaggcccgg cgctggtggc gctgggtcgg cgcgtggacc gcgacgtcgc cgcgcaggcg
      301 tgcaaaggcc agatcaccgt ggcggcgggc cagacgcctg aaaagcgcac gctgaccttt
      361 tacgacgcga agaccggcgc cgagttgaag ccgacggtgg actggggctc ggccctcacc
      421 ttgcgcgtgc agcgagagcg tgcgcgacct tgcggctact ggctggcgcc gagcgagaag
      481 gcggcagtcg aggcgctgcg tgcgctcggc gttcaggtca aatcaatgcc tggcgcacgc
      541 caggtcagcg cggagcgcta tgttttcaag agtgccgagc aaggccagcg gcaggacggg
      601 cggggcgcca ttcaggatgc cggcggcatc gtgaagaccg aagttgcgct ggccccggcg
      661 cgtgtgcggt tgcccgctgg cggctggacg gtgagcctgg cgcagccgct ggccgggctg
      721 gtgaccgctg cgctggagcc cgattcgcaa aacagctacg tcgccgcgcg catgatcgcc
      781 gtgccgaaag agggtgaggg cgatcggctg ctgcggctga tggcgccggt gcgctaacag
      841 cctacgcatg accagtttca tgacgccgcg ctctcgtgcc aaagaagcta cctctccgtc
      901 tgggagaggg ggtttctttt tcacacgtcg gccgtgtcaa gagatacgca aacgtcaata
      961 gcgccagcgc caagccacgg cgccagccgc atcgcccgcc catggcggta acttgaccgg
     1021 cacggcgtgg atgccggcca actgcgtcgc caacgcatcg ctccactcaa ccagcgccag
     1081 gccggcgcac ttcagccgtg cttcgtggca cgcccagtgc gacaagaaag ctcctgtatt
     1141 aatagctgtt tgcgcttgat ggacaagcgc tgacggccta ttcggttcca aatacagctg
     1201 tgcgacgcgc gccagttcag ccgcgtcggt gtgcacggcg aggcgcacca catcgacgcc
     1261 gaccagcccc gcgccgtgcc acgcgatgag cgacacgccg cgctcgtggc cgatggacag
     1321 gcccacgttc gcccaccgcg tggaatcgcc gacgtgggcc gacacgcggg gcgctgcgcc
     1381 gcgctgctgg ctgatggcga tgtcttctgt agccacgccg aacactgccg ccagccgcgc
     1441 gcgcagcgca tcgcgcgcgg cctggcgttg gtgcgcgcgt tcgtcctcgg ggtgtgcctg
     1501 cgcgcggtcg ggctgcggca gcgcccacac ctcaggcgcg ccggccgcat cccagccgtc
     1561 gaaccggcag cgcgccagcg cgagcgcgtc ttcggccatg ctgccaatca aaaatcaacc
     1621 aggctcaacc caatcgaaaa caccgtgcgc ttgaagttgt agtcgagcag gctgtcgcca
     1681 tagccgtgaa acagcgcggt gtacagccgc aggcccgaga agctgttgcc caggccgtcg
     1741 cccagcgagc gcgtccactc gatgcggccc gagccgcgct tgttgaagcc ggtgtaggtg
     1801 gcgcgcaggc tgttcttgct gtcgatgttc cagcccacgg tgacgtcgcc gcggcccagg
     1861 tagcgcgtca ggtcggggtt gttgtccttc gcggccgatt cggacaggcg cttccacagg
     1921 cgcatctgca cgttgaagcg gttgtccttt tcaaagccgg ccatcacgta ggcgcggttc
     1981 cagctgcgcg agagcgggtc gctttgcccg ttggactggt gcgccagccc cacgcccgaa
     2041 tagcgccaca gccagccgcc gggcagctgc agcgtggtcg ggtagacgta gatcagctcg
     2101 ggcatgtggt cggtgttgcg aaacggccgc gacagtctgc tgttgaacgc ctgccactcc
     2161 gactgctggc tgtaggcggc ccagagcgaa tcgcgcagcg tgccgtcctt gggggtcagc
     2221 aggccgctgg ccagcttggt gcgcaccgac aggtgcaggc gcatggcctg cttgctgtag
     2281 tcgatcgatt cggtggcgct gttgatcggg ttgggcgagg tgggctggcg gttgacttcg
     2341 tcgctgccgg caatcgccac cgtgttgggc cggaacccgc gcaggccaaa ggtggggcag
     2401 ctagagcccg attccagctc ccagaagcgc gagagctcgg agaactggcg gtcgcggcag
     2461 ccttgctcca gccccacgcc gatgatgccg gccgagccgg acggcgtggt gccgggcgca
     2521 gactcgggct gcgtgctggc cagcgcggcg gccacctcgg cccgcacgcc cgaggcctgc
     2581 gcgttgggcg acgcctgcac cgagcgcgcc ttgtcctcga tggcggtgac cagctcgcgc
     2641 tgctctcgcg cccagtcgtc gaagcaggcc aggcgctcgt tgttggccat gccggtgcat
     2701 tgctgccagg cggcggcttt ttcaacgcgc tgggcgacct cggcctgttt gggcgcggtc
     2761 tgcgcgctgg cgccgccgac cagcagcgcg agggccagag cggaaggatg aaagcgaggg
     2821 gtcacgagaa ggctccgttg caacaagggg cgcgtcgaag tgccaagcac cggcgcgatg
     2881 gcaggtgatt gtgcatggcg ggcgtggcat aggccgtcgc ggcggagcgg cggcgttgtt
     2941 ttgatgccgc gcgggttcgg gcagatcaca aacagccgga cgcgaaggac gcgaaaaatt
     3001 caagaaaact ttttcggctg cttctttcgc gtccttnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnngctg cggcgcgaca ccacggtggg ctgcaccgtc atccagccgc
     3181 cgtcgatgtg ctcgtcgccc agcatcacgg cgtcggcgat gaagcattgg tcgcccaggg
     3241 tcagcatgtc gggcaccacg cccagggcgg ttgacagctc ggtctccttg cccaccttgg
     3301 cgcccagcag gcggtaccac gccgcgctgt aaatggtggc gtagatgccg tgcagaaccg
     3361 ccaggctcga ttcctggatc tggctgacca gccacttggc cagatagcgg ttgctgtgga
     3421 tgggccacga accggctttc atcttgggca gcagcgccca gcggatgaag gccgacagca
     3481 gcacggtggc aaagatgaac accccgctgg ccggcagcgc gagcagaaag aacttgacca
     3541 gccgcagccc gaacgcctgc agcgccgtga tgctgccgtc gtccaccagc gggcgcaccg
     3601 acaggttggg cacgtccagc cagtcgatca gcaagaaggt ggggaacacc ggcatgaaga
     3661 acagcacggc gatcagcacc gcgccggtga tgaacaccgc ccgctcccac cacagccgcg
     3721 cggtcgtgac ggcggggcgc gccggcagcg tggccgggtc gaagtcgccg cgctcacgcg
     3781 ccggcgagcc gtgccacacc ttgcgcgccg gcacctgctg gccgctggcc agcgccgact
     3841 ggccttccag atgcccgtat tcgccgatgc gcgtgtcgcc ctcgatcacc acgtacgagc
     3901 cgacgttggc ctgcgcccca atgtcgatgg tgcccagatg cagcaggccg ccttcaaccc
     3961 gcgcgttctc caggttgacg acgttgccga tgctggcgcc ttcgccaatg cgcaccaggt
     4021 gcggcacgcg gatggtgacc gagcccaggt tgacctcggg ccctaccttg gcgccttgca
     4081 ggcgcagcca ggccgcgtac agcggcgaac cgttgatcat gtaggccggc accgcctcgc
     4141 tgatgcggtc ggccagccac cagcggtaat aggtcaggcc ccacagcggg tagctgccgg
     4201 gcttcaggtt gccggcgatc acgcgccgcg caaacagcgc gatggcaaag ttgagcacga
     4261 tggcgacgat gaaggtcagc accgatgcga acacggcctg gctgatcttg tcgtcggcgt
     4321 cgcccgtatt gaagtggtag gcgaagaagg gcgccagcca ctgcgccatg gtcagcagca
     4381 ccaaaaaagg aatcgccagc ccctgcgcga tgccgcagcg ccagcggcgc caggcgctgt
     4441 gcagcacgaa tgggcgatca gccgtggcct cggcgccgac catgccggcc tgcagcgcct
     4501 cggcgatcag gccgagcttg cggttctggt agatgtcggc caccgtggcc tgggcaaagc
     4561 gcgggtcctg gcgcagtgtg ctggtgatgc gcgcggcaaa cagcgaatgg ccgcccaggt
     4621 cgctgaaaaa atccagctcg cggcgaatcg gctggccggg gaagagtttt tcgagcgccg
     4681 caaacagcac ttcttcggcc ggcgtctccg gcgtgtcgct gccctcggcc ggcgcggccg
     4741 ccgacagcgg ctggcttttc agcaccttgc ggtcgatctt gccgctttgc aggcgcggca
     4801 tggcgggcag cgcctcgaag cgcgccggca ccatgtaggg cggcagcttt tcggcgaggc
     4861 ccgcgcgcaa atccttgatg gcgggcggcg tggcgcccga gggcacgtaa aaggccacca
     4921 gctggtcgat gtcgccctcc ttgcgcaaca gcacggcggt ggtgcccacg cccggctgct
     4981 ccgccagcac ggcctcgatc tcgcccagct caacgcgaaa gccgcggatc ttcacctggt
     5041 cgtcggcgcg gcccaggcac tgcatctgcg tgccgttttc gacccgcgcc agatcgcccg
     5101 tgcggtacag ccgcgcttcc tgttcattgc gcgcccaggg gttgggcaag aacttttcgg
     5161 ccgtcagatc gggccggccg aggtagcccg ccgccacgcc gggaccggtg atgcacagct
     5221 cgccggtgtc gccgaacggc aagatcttga gcgggggcac catgccgttc acgatgctgt
     5281 cggggtcgat cacctcgatg accaacagcc cgtagttggg cagcgcctgg ccgattgtca
     5341 ccggctcgcc ggccttcagc tccgcgaggg acgccgacac cgtggcctcg gtcgggccgt
     5401 aggtattgaa catctggcgc gccggcgtgg cccatttgtc aaccagcgcc tgcgggcaca
     5461 tctcgccgcc caggttgatg atgcgcaggc ccggcacgtc ttgggcaaac aacgccagca
     5521 gcgtgggcac ggcgtgcagc accgtcacgt cgttttcaat cagcgcacgc ggcagcgcct
     5581 cggggtcgcc ggcgatctct tttggcgcaa tccacagcgt ggcgccgacg aggtagctga
     5641 tccagatctc ctcgaagctc atgtcgaagg cgacggaaaa gccttggtaa accttgtcgt
     5701 cctcgcgcac ccccaaccgc gcgttttcgc tgcgcaggaa gtggctgatg ctgccctgcg
     5761 tgatggcgat gcccttgggc ttgcctgtgg agccgctggt gtagatcaca tacgcggtgt
     5821 gctccggcag cgcgccttcg cggcggcgca gcgcggtgcc ttcgggcagg gcgctgagca
     5881 gctcgctggc cgtcaggatt tgagccgaaa tgccgctttg agcttgcaca gcttgcgcgg
     5941 aaagctctga aatcaggagc gccttggccg atgcatcatc cagacaaacg gcgatgcggt
     6001 cggtcggcac gtcggcgtca aacggcagcc aggcggcgcc ggtcttggcg atgccgagtt
     6061 gcaacaccag cagctcgatg ccgcgcgggt gccacaaacc caccatgtcg ccggcgcgca
     6121 cgccggcttc gatcagccgg tgcgccacgc ggtcggcggc gtcgttcagc tcggcgtacg
     6181 tcaggtgccg ctcgccgaag atcagcgccg tcttgcggca cgcgcgcggc ggtggcttca
     6241 aagatgtcgg ccaggatctc gtggcgcagc aggtcgggcc gatcagggcc gtgcaggatg
     6301 gggaagtcgg gcatgcaaaa aaacggggcg cggtcggtgg cccactcacc cagcggcagg
     6361 cggcggcgcg gttgacaggc gaagagctgc attctgcctg ccgctacagt gcgcgcatgt
     6421 gtgccgcacc gccgttgttg ttgaacctgc agggcgcgcg ccgccgcctg ctctacgtca
     6481 cgctttttga ggggctggcg attgccatcg tgacgctggc attgctcgtc ttcaccggcg
     6541 cgggggcggc cagcgccggc gggctggcca tcggctcgtc ggccattgcg ctgggctgga
     6601 acctgatgtt caacatgctg ttcgagcgct gggagcgccg ccagccgacg cgcgggcgca
     6661 gcgcggcgcg gcgggtggcg cacgccatcg gctttgaagg cggactgctg ctgtttctgg
     6721 tgccgttttt cgcctggtgg ctgggcgtga gcctgtggca ggcgctggtg ctggacatcg
     6781 gcttcttgct gttctttctg gtctacaact ttgtctttgc ctgggcgttc gaccggctgt
     6841 tcgggctgcc ggccggggtg gggtgatttc aagggtttcg ggctctggcg cttgtccgcc
     6901 cagcgcgagc agctatcaaa attgtattga tcaaggcggc ctgctgcggc atcgcgcgac
     6961 aatccatccc atgctgcaac tcgccgatat ccaagccgcc gccgcccgtc tgcagggcca
     7021 ggtgctcgac acaccctgcg tcgaatcgcg cacgctctcg caggtgacgg gcgcgcaggt
     7081 tttcttgaag atggagaacc tgcagttcac cgcctcgttc aaggagcgcg gcgcgtgcaa
     7141 caagctggtg cagctttccg ccgaagagcg cgcgcgcggc gtggtggcca tgagcgccgg
     7201 caaccacgcg cagggcgtgg cgtttcatgc gcagcgcttg ggcatccgcg ccgtcatcgt
     7261 catgccgcgc ttcacgcccg gcgtgaaggt ggcccgcacg cgcggctttg gcgcggaagt
     7321 catcctgcac ggcgacacgc tcgacgctgc cagcgagcac gcacgcaccc tggcgcgcga
     7381 gcaagggctg acttttgtgc acccgttcga cgacgaagcc atcatggccg gccaaggcac
     7441 gctggcgctg gaaatgctcg ccgagcagcc cgatctggac acgctggtgg tgccgatcgg
     7501 cggcggcggc ctgatcagcg gcgtggcgac ggcggccaag gcgctgaaag ccggcatccg
     7561 cgtggtcggc gtgcagacgg cgcgctttcc cggcgcggtg aacgccatca ccggcagcgc
     7621 gcacgcgcag ggcgccagca ccatcgccga aggcattgcc gtcagcagcc ccggcgcgca
     7681 gtgcctgccg ctgattcgcc agcacgtcga cgaactgctg ctggtggacg agggcgacat
     7741 cgagcaggcc atcgtcatgc tgctggagat cgagaaaacg ctgtccgaag gcgcgggcgc
     7801 ggcggcgctg gcggcgttgc tgcgttatcc ggagcaattc aaggggcgca aagtgggcct
     7861 ggtgctgtct ggcggcaaca tcgaccccat gctgctggcc gccatcatcg ggcgcggcat
     7921 ggtgcgctcc ggccggctgg cgcgcatcgt cgtcagcgcg cgcgacgtgc ccggctcgct
     7981 ggcgcgcgtg accacgctgg tggccgaagc gggcgccaac gtgcaggaga ttcaccacca
     8041 gcgcgccttc accctgctgg cggcgcagaa cgtcgagatc gagatggtgc tgcaaacgcg
     8101 cgacacggcg cacgtgcagg agctgctggc gcggctggcg gccgaggggt tggaggcgcg
     8161 ctggctgggc gcggcgcggg tgagcgattg aaggtcaaaa cagcggctga cgcttgatag
     8221 tcaagcgcga gcagctatgg ttttgagagt gttttgaacg caaagggcgc agaagcttcg
     8281 caaaaggcgc agaaattttt caaatttctc ttctgcgtct tttgcgccat ctctgcgccc
     8341 tttgcgttca agggtatggc tgagcgtccc cacgacactc cgcgcacgtg ccatgcaccg
     8401 ccagatcgac gcgcgccgcc tgatgcccat attcggccag cgtgcggcgc agcgcgtcgg
     8461 ccacggcttg cgtgggggcg tcggcgtctt gcagcggaaa gtgcgtgtgg caggcgtcgc
     8521 attcgaaatg cggcacggcg gcggtgctcg cgcgctcatc gatgcccatg cccatgccca
     8581 gccagcggaa gcgccacgtg cgctcgctcg gcgccgtgtg gcgcgccagc aggccggcgg
     8641 caaccaggcg atcaagcagg cggtacaggg tgacggcgtt catcggcgcg cccgactgcg
     8701 ccagttgatc tgccacttct gcgtgcgtcg gcgcccagcc ggcggcggca ccatcgaaca
     8761 gctcggccac gcccagcacg gcgcgcgtgc tgcgcaggcc ggcctggtgc aggcgctggc
     8821 tgagggcggt ggggtcgagg tcgggcatgg gcttcaataa cgcaactgac ttgcgatatg
     8881 atcgcgcgct tggcgctttg atgcaagtca gttgcatttt aggccgctca acgaagccat
     8941 gacacccccg ccctcgctgc ccaaacgccc gccgctgctg ctgcgcgcgg ccggctggcg
     9001 cctgctggtg gccgccggtt ttagcgcgct gctgctgttg ctgtggcgtt gggcggtgat
     9061 ttgacatgct cgcgccccaa ccgatcacgc tgcgcgacct cacgctgggc tacgacggcc
     9121 acccggcggt gcaccacttg tcgggcacca tcgcgcccgg cgcgctgctg gcggtggtgg
     9181 ggcccaacgg cgcgggcaag tcgacgctga tcaaggcgct ggccgggttg ctgacgccca
     9241 tcggcggcca catcgacggg ctggccggcc agcgcgtggc ctatctgccg cagcaggcgg
     9301 cgctggagcg cggctttccg attggggttg gcgaattcgc ggccatgggc ttgtggcacg
     9361 aaacgggtgc gtttggcggc ttttcgcgcg cgcagcgcga gcgggtgcgc gcggcgctgg
     9421 cggcggtcgg gctcgaaggc cacgcgcgcc agccgatcga cacgctgtcg ggcggccaat
     9481 tgcagcgcgc tttgtttgca cggctgatgc tgcaagatgc gcccgtgttg ctgctggatg
     9541 aacccttttc ggccatcgat cagcgcacca cggtcgattt gctgcaactg ctgcggcagt
     9601 ggcacgcggc cggcaagacg gtgctggcgg tgctgcacga cttgcaccag gtgcgcgatg
     9661 cctttcctga aaccctgatg ctggcgcgcg agctggtggc gtgggggcgg acggatgagg
     9721 tgctgacgcc ggccaacctg cagcgcttgc gcgcgatgcc ggaggcgttt gacgaccggg
     9781 cggcggtgtg cgaggtgccc gccgccggta cgccgcacga tcatggtcac gcacacgatc
     9841 atcagcacca tcacggcgcc acggagccgc gctcatgacg cccgaaatcg ccgacaccgg
     9901 cctgtgggcg ctgctgatcg cgcccttgac cgaattcggc ttcatgcggc gcgcgctggt
     9961 cggctgcgtg gcgctgtcgc tcagttgcgc gccggtcggc gtgtttctgc tgctgcgccg
    10021 catgagcctg acgggcgatg ccatggcgca cgccatcttg ccgggcgctg ccatcggcta
    10081 cctgctggcg ggcctgtcgc tgccggcgat gaccttgggc ggcatcgtcg ccgggctgct
    10141 ggtggccatc ggctcggggc tggtggcgcg ctccaccgtg ctgcgcgaag acgccagcct
    10201 ggccgcgttc tacctgctgt cgctggcgct gggggtgctg atcatctcca cgcgcggctc
    10261 cagcgtcgat ttgctgcacg tgctgttcgg caccgtgctg gcgctgggcg atggcgcgct
    10321 ggggctgctg gtgggcattg cgattaccac catggccgcg ctggcgctga tgtaccggcc
    10381 gctggtgctc gaatgtctgg acccgcagtt tctgcgctgc gtcagcccgg ccagcccggt
    10441 ggcgcactac ggttttctgg tgctgctggt gatcaacctg gtggccggct ttcatgcgct
    10501 gggcacgctg atggccgtcg gcatcatggt gctgcccgcc gccaccgcgc ggctgtgggt
    10561 gcgcagcgtg ccggcgctga tggcgctggc cgcggcactg gcgctggcgg cgggcgtggc
    10621 ggggctgctc atcagctacc acgccgactg gccgaccagt ccgaccatcg tgctggcgct
    10681 gggcgcggcg tatctcgtgt cgctggtcgt cgggcgatac ggcgcgtggc gcggcgggca
    10741 gcatgggcgg cggcgccatc tgcgggcttg agcttttgaa gtcttttcgt tctctggcgc
    10801 ttgcctgtta agcgcgagca gctatggttt ttgaatgacg tgcctgcggc gcatgacctg
    10861 ggcctgtggc ctttgatgac ggcgctgcgc gccatgacgg gactcacgtc atttcgtcat
    10921 ttcgtcatca aaacacaccg cagcgccggc cgggcaagcg cagacagcta tcaattcagt
    10981 agtctcagga gtttacccat gtccttgttg attccccgcc gcatcgcctt gatcgccgcg
    11041 ctggccagcg cgatggcagc gcccgcccac accgccgagc cgctgcgcgt ggtcgccacc
    11101 ttcagcatcc tgggcgactt ggtgcagcag gtcggcggcc agcgcgtggc ggtgcagacg
    11161 ctggtcggcc tgggcggcga tgcgcacgtc ttccagccga cgcccacgca cgcgcgccag
    11221 gtcgggcagg cgcagctggt ggtttccaac ggcctgggtt atgaaggctg gatgccgcgc
    11281 ctgctgcaaa gcaccggcta caaaggcctg caagtcatcg ccaccaaggg cgtgcggccg
    11341 attgaaggcg gcaaggacca cgaccacgat catgaccacg accgcgccga cccgcacgcc
    11401 tggcaaagcg tgcccaacgc catcgtctac gtggccaaca tcgccgacgg cctgtgcgcc
    11461 gccgacgccg cgggctgcgc ggactaccgc actggcgccg cgcgcaccac cgacgcgctg
    11521 aaaaagctcg acgccgggat ccgcgccgcc tgggcgccca tcgcgccggc agagcgcaag
    11581 gtgatcacct cgcacgcggc gtacgcgtac tatgggcaag cgtacggcgt gcgctttctg
    11641 tcgccgcaag gcgtcagcac cgagagcga
//
LOCUS       DAOMVO010000041        11534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1323_length_11534_cov_12.014374, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000041 DAOMVO010000000
VERSION     DAOMVO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1323_length_11534_cov_12.014374"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKV30_03620"
     CDS             complement(<1..495)
                     /locus_tag="PKV30_03620"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRL29343.1"
                     /translation="MALIQYLTQIEFDYGALAQIQAHCQRAGITRALVVTDKGVRAAG
                     VLQPVLDALGDVAVAVFDDVPSNPTEAAVRGAVAVYEKERCNGLIAVGGGSAIDCAKG
                     VAIAATHPGPLKTYATIEGGSPKITDAAAPLIAVPTTAGTGSEVARGAIVIVDDGRKL
                     GFHSW"
     gene            564..1469
                     /locus_tag="PKV30_03625"
     CDS             564..1469
                     /locus_tag="PKV30_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HRL29344.1"
                     /translation="MKIPLLPALIASAALGLLAGCANNAAQVVAATPGCLDDVAVNRL
                     VADYNARRPAADLPATLTMADAACTRSRLQQRLAGQAGRLVGYKAGLTNPAVQKRFNT
                     DQPVWGALYTDMLLGNGATVDAAFGARPLYEADLLVRVKDAAINNAKTPAEVLAHVDQ
                     VIPFIELPDLVVESPPKLNGAGISAINVGARLGVRGTPLVVPADAAGQARLLDQLRDM
                     NVRLVDAQGAQLGGGKGSDVLGHPLNAVVWLAGALQEQGLSMRPGQVISLGSFSALLP
                     PKPGLQATVHYDGVTGLQPVSVRFR"
     gene            1735..2556
                     /locus_tag="PKV30_03630"
     CDS             1735..2556
                     /locus_tag="PKV30_03630"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HRL29345.1"
                     /translation="MPAFRIAAAALAALILSPLAQAQYGQPIGRPLDAGPVMPWGQPA
                     YGGTVTGGGQGVVCESRDGGFRECGTPFSGPPAIVRTLSASGCVQGQTWGSRAPGSVW
                     VTAGCRAEFVDGYAAQGGYPGHYGGAYADGQLLRCESDQGRQRECRAPVRTRLTLVRQ
                     LSDAPCVEGQTWGSQRSGRVWVRGGCRGDFAPQGARPGGPAYDQPSYGYGAGQTITCE
                     SLDRRSNACGWDARWGHPVLVETLSADACLEGRTWGYDGRQIWVDRGCRARFGSR"
     gene            2744..3718
                     /locus_tag="PKV30_03635"
     CDS             2744..3718
                     /locus_tag="PKV30_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29346.1"
                     /translation="MNHPPDIHARLTPAMRLLVERMAQAPHPPMHTLSAADAKATYAK
                     SIGVLDVPKPDLARVEDLHIPARDGFQLRARLVAPSHDAGLPVLLYTHGGGFTIGSIE
                     SHDTFCRVLSQLSGAAVLSLDYRLAPEWRFPTAVHDAWDALHWLAGAGGAALGLDTSR
                     IAVGGDSAGGTLAAVSALHARDEGLKLALQLLIYPGTTDHQDTDSHAVFEHGPVLDKV
                     LIDWFFHHYIDAVDRSDWRFAPLKSAEVDDVAPAWVGLAECDPLVDEGILYADKLRAA
                     GVPVELEIYRGVIHGFITMGRAIAEARELQRDAAAALRQAFGLPLSGA"
     gene            3797..4663
                     /locus_tag="PKV30_03640"
     CDS             3797..4663
                     /locus_tag="PKV30_03640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HRL29347.1"
                     /translation="MKPADFRFHHHLRVRWAEVDMQKIVFNPHYLMYFDCAIADYWRA
                     LAMPYEAAMQLLDGDIFLRKTTVEFNASARMDDRLDVALRCDRIGNSSMTFVGAIFRD
                     GQLLNAGELVYVFADPATQTSRPVPAPLRALIEAYESGQPVTQVQTGDWATLQQPASS
                     VRTAVFVEEQGIKREDEWDAADATAVHAVVTNLLGMPVATGRLLPDGAANSGSARIGR
                     MAVDRTLRGSGVGRQVIDALEQAARARGDQRIVLSAQRSAEGFYQRLGYQLVGEPYEE
                     VGIAHIGMARLL"
     gene            complement(4680..5270)
                     /locus_tag="PKV30_03645"
     CDS             complement(4680..5270)
                     /locus_tag="PKV30_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29348.1"
                     /translation="MHQAQALQAARSALATAVVGLLIALPSVAGVQFVAPEGPEGQVA
                     PAPVPVGEPIAAQRLRHKVHFIYMGGDDCPSCVVWRRGELPRLALSDAFRASRYSFVN
                     KPILSGVPGLFLLPYEVKPYKAVLDQASGGNMGSPHYAILVDGVLHHYGFSAPPAERM
                     EQMLRAALTDGRWPEPTRCLMRRPRAVTQCAESVPG"
     gene            complement(5414..6358)
                     /locus_tag="PKV30_03650"
     CDS             complement(5414..6358)
                     /locus_tag="PKV30_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRL29349.1"
                     /translation="MSDIASPTPAGLNGIATPLPHLGVMRAKGDDAASFLQGQLTQDV
                     VLMKPGEARLAAFCNAKGRMLASFQVLKVAADELLLVTSADTLAATLKRLSMFVLRAK
                     AKLSDATADVALAGLLGDAAVSIAARARPTSASGQFDHEFTIDLPTADGTPRALWLAP
                     AGTPAPAAPPVSEADWLLAEVRAGVARITQPVVEAFVPQMLNYESVQGISFKKGCYPG
                     QEVVARSQFRGAIKRRAFVGQVNGDAAAGQEVFAAGDPDQPVGRIAQAAARPGGTSAV
                     IVSVQTADVAAGHALALGGAGGPVLEGLHLPYPLLEDI"
     assembly_gap    6440..6539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6540..7367
                     /gene="mltG"
                     /locus_tag="PKV30_03655"
     CDS             <6540..7367
                     /gene="mltG"
                     /locus_tag="PKV30_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRL29350.1"
                     /translation="GWYFRLAGREQTIKPGDYAVTRGMTPADLLGKLVRGDRIVLTVT
                     LVEGWTFKQFREALAKAEHLRHDTQALTAAEIMKTLGKPGLAAEGRFFPDTYHYFKNA
                     SDIDVLRQSLQLMDRRLQAAWDARAPGLPLKSADEALVLASVVEKETGLPKDRPEIAG
                     VFINRLKIGMRLQTDPTVIYGLGDAFDGNLRRSHLQADTPFNTYTRAGLPPTPIAMPG
                     KASLMAAVQPAQTKALYFVARGDGSSHFSGSLEEHNRAVNKYQRGGAVSAASAAGGN"
     gene            7370..8026
                     /gene="tmk"
                     /locus_tag="PKV30_03660"
     CDS             7370..8026
                     /gene="tmk"
                     /locus_tag="PKV30_03660"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRL29351.1"
                     /translation="MAQPGVFISFEGIDGAGKSTHIEALAGAFRAAGRTVVLTREPGG
                     TALAEKIRALVLHEPMGPLTEALLMFAARRDHIEQVIAPALARGEVVLCDRFTDATFA
                     YQGGGRSFDWNSLSKLELLAQDRQAPEANLAHNDLLQPDLTIWFDLPPAVAAERLAGA
                     RVPDRFESQPAAFFDAVRAGYARRAAEAPARFARIDASQPRDAVGADVRAAVQGRGWL
                     "
     gene            8017..9015
                     /locus_tag="PKV30_03665"
     CDS             8017..9015
                     /locus_tag="PKV30_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRL29352.1"
                     /translation="MAVTAPWIQRQLEALLAQRGHAWLLHGPSGLGQFELAYALVRAW
                     LCDQPTPQGACGQCASCHGIDVHTHPDLAALMPETDMLARDWPLPEKAQSDIDDKKRK
                     PSKEIRVDAMREAIEFAQRTSARGRGKAVLVYPAERMNAITANALLKTLEEPAGDMRF
                     VLATDAAHLLLPTIRSRCLAHTMAWPDEAEALAWLATQGVPAEEGALLLRAAGGRPAE
                     ALALAQTKGSAQSWAQLPRALARGDTAALADLPLAEAVSRLQKLCHDLLALQSGATPR
                     FFAAADLPAQPLSAAALGRWWQQLGRSARTAEHPLNPGLAAEFLVSSARQALNSRR"
     gene            9037..9411
                     /locus_tag="PKV30_03670"
     CDS             9037..9411
                     /locus_tag="PKV30_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRL29353.1"
                     /translation="MSTAPAPVASSTPRPSVIQLAIKEKAALYAAYIPMFAEGGIFIP
                     SAREHQLGDDVYVLITLPDDPQRYPVAGKVAWITPARSTGNRTQGVGIRFPKDEKSQQ
                     LKVKIEQILGAALGSDRPTQTI"
     gene            9522..10328
                     /locus_tag="PKV30_03675"
     CDS             9522..10328
                     /locus_tag="PKV30_03675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRL29354.1"
                     /translation="MFTDSHCHLTFPELAGDMPGVRAAMAAARVTRALCISTTMEEFP
                     SVQALATEYDNFWASAGVHPDSEDVHEPSVAELVAATQMPKVVAIGETGLDYYQMEER
                     KGGRGIADMEWQRERFRTHIRAARQVGVPLVIHTRAASDDTLAILREEGEQGGAGSAG
                     GVFHCFTETREVARAALDLGFYVSLSGIVTFKNARELHEVATLIPCDRLLIETDSPYL
                     APVPYRGKINTPAYVPHVAARLAELRGTSAEHIGELTSANFTRLFKRVAS"
     gene            10325..11002
                     /locus_tag="PKV30_03680"
     CDS             10325..11002
                     /locus_tag="PKV30_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRL29355.1"
                     /translation="MTKFLQALLAAALALLASVAVADDRGAFERAIKQDNDSAVRSML
                     SRGFDPNTRNEKGAPGLYMALQEGSLKVARVLIDSPRLKPEDRNASDESPLMMAALKG
                     ELDLAKRLIAKGADVNKPGWTPLHYAATSGHLDMIRLLLEEHAFIDAQSPNGTTPLMM
                     AASYGSPEAVKLLVEEGADIAMRNQKQMTAMDFARRAERRDAVELLTQAERYKAQSRP
                     PAERGRW"
BASE COUNT         1841 a         3937 c         3890 g         1766 t
ORIGIN      
        1 ccagctgtga aagcccagct tgcgcccgtc atcgacgatg acgatggcgc cgcgtgccac
       61 ctcgctgccc gtcccggccg tggtgggcac ggcgatcagc ggcgcggcgg cgtcggtgat
      121 cttggggctg ccgccttcaa tggtggcgta ggtcttcagc ggcccggggt gcgtggcggc
      181 aatggccacg cccttggcgc agtcgatggc gctgccgccg ccgacggcga tgaggccgtt
      241 gcagcgctct ttttcataca ccgccacggc gccgcgcacg gcggcctcgg tggggttgga
      301 gggcacgtcg tcaaacaccg ccactgccac gtcacccagt gcatccagca ccggctgcag
      361 cacgccggcg gcgcgcacgc ccttgtccgt gacgaccagc gcgcgcgtga tgcccgcgcg
      421 ctggcagtgc gcctggattt gcgccagcgc gccgtagtcg aattcgattt gcgtcaggta
      481 ttggatgagg gccatgggag tttttccaaa gtacgatgtg gcggcttcaa ttattcaatc
      541 aaacgccgag gagacccgcc accatgaaga ttcccctact gcccgccctg atcgccagcg
      601 ccgccctggg cctgctggcc ggctgcgcca acaacgccgc gcaagtggtg gccgccacgc
      661 cgggttgcct ggacgatgtg gccgtcaacc gcctggtggc cgactacaac gcgcgccgac
      721 ccgccgccga cctgcccgcc acgctgacca tggccgacgc cgcctgcacg cgcagccgcc
      781 tgcagcagcg gctggcggga caggccgggc gcctggtggg ctacaaggcg ggcctgacga
      841 acccggcggt gcaaaagcgc ttcaacaccg accagccggt gtggggcgcg ctctacaccg
      901 acatgctgct gggcaacggc gccactgtcg atgccgcctt tggcgcccgc ccgctgtacg
      961 aggccgacct gctggtgcgc gtgaaagacg ccgccatcaa caacgccaag acgccggccg
     1021 aggtgctggc ccatgtcgat caggtcatcc ccttcatcga actgcccgac ctggtggtcg
     1081 aaagcccgcc caagctcaac ggcgccggca tcagcgccat caacgtcggt gcgcggctgg
     1141 gcgtgcgcgg cacgccgctc gtggtgccgg ccgacgctgc tggccaggcg cgcctgctcg
     1201 atcaattgcg cgacatgaac gtgcgcctgg tcgatgcgca aggcgcgcaa ctgggcggcg
     1261 gcaagggcag cgacgtgctg ggccacccgc tgaacgccgt cgtgtggctg gccggtgcgc
     1321 tgcaagagca gggcctgtcg atgcggccgg ggcaggtcat cagcctgggt tcgttctcgg
     1381 ccctgctgcc gcccaagccg ggcctgcagg cgaccgtgca ttacgacggc gtgactgggt
     1441 tgcagccggt gagcgtgcgc tttcgttgac cgccgcgcgg taacagaaag cgtagatcgc
     1501 tatggttagc atagctgctc gcgcttgata gtcaagcgcc agcggccaat ttcacttgaa
     1561 gctcttaaga cagatgcaca tccaatcggg agccaacgga acaggagcag ggttgctgtg
     1621 ggagcgacct gaaatacgcg gcccatcgag caatttcatt gacgaaccgg cagccatgcc
     1681 gcccgcgttg agcccgtgcc cgccgaacac cgctttttca ctggaggatc gttcatgccc
     1741 gcattccgca tcgccgccgc cgcgctggcg gcactcatcc tgtcgccgct ggcgcaggcg
     1801 caatacggcc agccgatcgg ccggccgctc gacgccggcc ccgtcatgcc gtggggccag
     1861 ccggcgtatg gcggcaccgt caccgggggc ggccagggcg tggtctgcga aagccgtgac
     1921 ggcggattcc gcgagtgcgg cacacccttc agcgggccgc cggccatcgt ccgcacgctg
     1981 tcggcgtcgg gctgcgtgca gggccaaacc tggggcagcc gcgcgccggg ctcggtgtgg
     2041 gtcaccgccg gctgccgggc cgagtttgtc gacggctacg ccgcgcaggg cggctatccg
     2101 ggccactacg gcggtgctta tgccgacggc caattgctgc gctgcgagag cgaccaaggc
     2161 cgccagcgcg agtgccgcgc gccggtgcgc acgcgcctga cgctggtgcg ccagctgtcc
     2221 gacgcgcctt gcgtcgaggg ccagacctgg ggcagccagc gcagcggccg cgtgtgggtg
     2281 cgcggcggct gccggggcga tttcgcgccg cagggcgcgc ggcctggtgg gccggcgtac
     2341 gaccagccca gctacggtta cggcgccggc cagaccatca cctgcgaaag tctagaccgc
     2401 cgcagcaacg cctgcggctg ggacgcccgc tggggccacc cagtgctggt ggagacactg
     2461 tccgccgacg cctgcctgga gggccgcacc tggggctacg acggtcggca gatctgggtc
     2521 gatcggggct gccgggcgcg cttcgggagc agatgacgct cgatccgttg cgatgacgaa
     2581 atgacgtcgc tgcgcttcaa tgacgaaatg accaagcgct cgtcgtggcg cgcagcgccg
     2641 tcatcgatgg ctgccgccga ggtcatgcgc cgcaaggcgc gtcatctaca aaaaccatag
     2701 ctgctcgcgc tttccagaca agcgctagag gcctttttct ttcatgaacc atccccccga
     2761 catccacgcc cgcctgaccc ccgccatgcg cctcttggtc gagcgcatgg cgcaggcgcc
     2821 gcacccgccc atgcacacgc tgagcgcggc agacgccaag gccacgtacg ccaaatccat
     2881 cggcgtgctc gacgtcccca agcccgatct ggcgcgcgtg gaagacctgc acatccccgc
     2941 gcgcgacggc tttcaactgc gcgcgcgcct ggtcgcgccc agccacgacg ccggcctgcc
     3001 ggtgctgctg tacacgcacg gcggcggctt caccattggc agcatcgaat cgcacgacac
     3061 cttttgccgc gtgctcagcc agttgagcgg cgccgccgtg ctgtcgctcg actaccggct
     3121 ggcgcctgag tggcgctttc ccaccgccgt gcacgacgcc tgggacgcgc tgcactggct
     3181 ggccggcgcg ggcggcgcgg cgctgggcct ggacacatcg cgcatcgccg tcggcggcga
     3241 ttcggccggc ggcacgctgg cggccgtcag cgcgctgcac gcgcgcgacg agggcctgaa
     3301 gctggcgctg caactgctca tctaccccgg caccaccgac caccaggaca ccgattcgca
     3361 cgccgtgttc gagcacggcc cggtgctcga caaggtgctg atcgactggt tcttccacca
     3421 ctacatcgac gccgtcgacc gcagcgactg gcgctttgcg cccctgaaaa gcgccgaggt
     3481 cgacgacgtc gcgcccgcct gggtgggtct cgcagaatgc gacccgctgg tcgatgaagg
     3541 catcctctac gccgacaagc tgcgcgccgc gggtgtgcct gtcgagctgg agatttaccg
     3601 cggcgtgatc cacggcttca tcaccatggg ccgcgcgatt gccgaggcgc gggagttgca
     3661 gcgcgatgcg gcggcggcgc tgcggcaggc gttcgggttg ccgctgtctg gcgcctgaaa
     3721 gactatgatt tatgtagctg ctcgcgcttt gcctgcaagc gctggaggcc ttttttaatt
     3781 ttttagagac ccaaccatga agcccgccga cttccgcttt caccaccact tgcgcgtgcg
     3841 ctgggccgag gtggacatgc aaaaaatcgt cttcaacccg cactatctga tgtacttcga
     3901 ctgcgccatc gccgactact ggcgcgcgct ggccatgccg tacgaggcgg ccatgcagct
     3961 gttggacggc gacatctttc tgcgcaagac cacagtcgaa ttcaacgcct cggcgcgcat
     4021 ggacgaccgg ctcgacgtgg cgctgcgctg cgaccgcatc ggcaattcgt ccatgacctt
     4081 tgtcggcgcc atcttccgcg acggccagtt gctcaacgcg ggcgagctgg tctacgtgtt
     4141 tgccgacccc gctacgcaga ccagccggcc ggtacccgcg ccgctgcgcg cgctgatcga
     4201 ggcgtacgag tctggccaac ccgtcactca agtgcagacc ggcgactggg cgacgttgca
     4261 gcagccggca tccagcgtgc gcacggccgt attcgtcgaa gagcagggca ttaagcgcga
     4321 ggacgagtgg gatgccgccg acgccaccgc cgtgcacgcc gtggtcacca acctgctcgg
     4381 catgccggtg gccaccgggc gcctgctgcc cgacggcgcg gcgaacagcg gcagcgcgcg
     4441 catcggccgc atggcggtcg accgcacgtt gcgcggcagc ggcgtgggac ggcaggtgat
     4501 cgacgcgctg gagcaggccg cccgcgcgcg tggcgatcag cgcattgtgc tgagcgcaca
     4561 gcgcagcgcc gaagggtttt atcagcgcct gggctaccag ctggtcggcg agccgtacga
     4621 agaagtcggc atcgcgcaca tcggcatggc gcgcttgctg tgatcaaggg cgctgcggtt
     4681 cagcctggca ccgattcggc gcattgggtg accgcgcgcg ggcgccgcat caggcagcgc
     4741 gtgggttcgg gccagcggcc gtcggtcagc gccgcgcgca gcatctgctc catgcgttcg
     4801 gccggcggcg cgctgaaacc gtagtggtgc agcacgccgt ccaccaggat ggcgtagtgc
     4861 ggtgagccca tgttgccgcc gctggcctga tcgagcaccg ccttgtaggg cttgacctca
     4921 tagggcaaca gaaacaggcc gggcacgccg gagaggatgg gcttgttgac aaagctgtag
     4981 cggctggcac gaaaggcgtc gctcagcgcc agccgtggca gctcgccacg ccgccacacc
     5041 acgcacgacg ggcaatcgtc gccgcccatg tagatgaagt gcaccttgtg gcgcaggcgt
     5101 tgcgccgcaa tcggctcgcc caccggcacg ggcgcggggg ccacctgacc ctcgggcccc
     5161 tcgggtgcca cgaactgtac gccggcaacg ctcggcaggg cgatcaatag gcccaccacg
     5221 gcggtcgcca gcgccgagcg cgccgcttgc agtgcctgtg cttggtgcat gtgacgccct
     5281 cccgtgcgcg tcggcagcgg tgacggcggt gaggtcgccg cgccagacaa actgattttt
     5341 tacagtgtgt aacgcctgcg gcggcgcgtc aagctggtgt gcgcgcgttg cttcgccggc
     5401 aagggggagg ggctcaaatg tcctccagca atgggtacgg caggtgcagg ccctccagca
     5461 cggggccgcc cgcgccgccc agcgccagcg catggccggc cgccacgtcg gccgtctgca
     5521 ccgaaacgat gacagcgctg gtaccgcccg gccgagcggc ggcctgggcg atgcggccca
     5581 ccggctgatc ggggtcgccc gctgcaaaaa cctcttgccc ggccgcagca tcaccgttca
     5641 cctgacccac aaacgcccgc cgcttgatcg ccccgcgaaa ctgactgcgc gccaccactt
     5701 cctggcccgg gtagcagccc ttcttgaagc tgatgccctg caccgattcg tagttcagca
     5761 tctgcggcac aaaggcttcg accaccggct gcgtgatgcg cgccacgccg gcgcgcactt
     5821 cggccagcag ccagtcggcc tcgctgacag gcggcgcagc gggcgcgggc gtgccggcgg
     5881 gcgccagcca cagcgcgcgc ggcgtaccgt cggcggtggg caggtcgatg gtgaattcat
     5941 ggtcaaactg gccgctggcg cttgtcggac gtgcgcgggc agctatcgaa accgcagcat
     6001 cgccgagcag cccggcgagc gccacgtcgg ccgtggcgtc gctcagcttg gccttggcgc
     6061 gcagcacgaa catcgacagg cgcttgaggg tggccgccag cgtgtcggcg ctggtgacca
     6121 gcagcagctc gtcggcggca accttgagca cctgaaagct ggccagcatg cggcccttgg
     6181 cgttgcaaaa ggcggccagg cgcgcctcgc cgggcttcat caacaccacg tcctgcgtga
     6241 gctggccttg caagaagctg gcggcatcgt cgcccttggc gcgcatcacg cccaggtggg
     6301 gcaggggggt ggcaatgccg ttcaggccgg cgggggtcgg tgaagcgatg tcagacatgg
     6361 gctgaattat catggccggc tcgtttttcg accgcctgcg accccgtgcg aaccttactc
     6421 aaactgctgg ctttgctgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     6541 gctggtactt tcgcctggcc gggcgcgagc agaccatcaa gccgggcgac tacgccgtca
     6601 cgcgcggcat gacgccggcc gacctgttgg gcaagctggt gcgcggcgac cgcatcgtgc
     6661 tgacggtgac gctggtcgag ggctggacct tcaagcagtt ccgcgaagcg ctcgcaaaag
     6721 cagagcacct gcgtcacgac acccaagcgc tgacggccgc cgagatcatg aagaccctgg
     6781 gcaagccggg cctggcggcg gaggggcgct tcttccccga cacctaccac tacttcaaga
     6841 acgccagcga catcgacgtg ctgcgccagt cgctgcaatt gatggatcgg cgcctgcagg
     6901 ccgcgtggga cgcgcgcgcg cccgggctgc cgctgaaaag cgccgacgag gcgctggtgc
     6961 tggccagcgt ggtcgaaaag gaaaccggcc tgcccaagga ccggcccgag attgccggcg
     7021 tgttcatcaa ccgcctgaag atcggcatgc gccttcagac cgacccgact gtgatctacg
     7081 gcctgggcga cgcctttgac ggcaacctgc gccgcagcca cctgcaggcc gacacccctt
     7141 tcaacaccta cacccgcgcc ggcctgccac cgacgccgat cgccatgccg ggcaaggcct
     7201 cgttgatggc ggcggtgcag ccggcgcaga ccaaggcgct gtactttgtg gcgcgcggcg
     7261 atggcagcag ccatttcagc ggctcgctgg aggaacacaa ccgggcggtc aacaagtacc
     7321 agcgcggcgg cgcggtcagc gcggcatcgg cggcgggcgg caattgacga tggcgcagcc
     7381 gggtgttttc atcagcttcg aaggcatcga cggcgccggc aagtcgacgc acatcgaggc
     7441 gctggcgggc gcctttcgcg ccgccggccg caccgtggtg ctgacgcgcg agccgggcgg
     7501 cacggcgctg gccgaaaaaa tccgcgcgct ggtgctgcac gagccgatgg gcccgctgac
     7561 cgaggcgctg ctgatgtttg ccgcgcggcg cgaccacatc gagcaggtga ttgccccagc
     7621 gctcgcgcgc ggcgaggtcg tgctgtgcga ccgcttcacc gacgccacct ttgcctacca
     7681 gggtggcgga cgcagctttg attggaattc actatcaaaa ctggagctgc tggcgcagga
     7741 tagacaagcg ccagaggcca atttggcaca taacgacctg ctgcaacccg acctgacgat
     7801 ctggttcgac ctgccacccg ccgtggcggc cgagcggctg gccggcgcgc gtgtgccgga
     7861 tcggtttgaa tcgcaacccg ccgctttctt cgacgccgtg cgcgccggct acgcacgccg
     7921 cgctgccgag gcgccggcgc gctttgcccg catcgacgcc agccagccgc gcgacgctgt
     7981 cggcgccgac gtgcgcgccg ccgtgcaggg cagggggtgg ctgtgacggc gccgtggatt
     8041 cagcgccagc ttgaagcgct tctggcccag cgcggccacg cctggctgct gcacgggccg
     8101 tcgggcctgg gccagtttga gctggcctat gcgctggtgc gcgcctggct gtgcgatcaa
     8161 cccacgccgc aaggcgcctg cggccaatgc gccagttgcc acggcatcga cgtgcacacg
     8221 caccccgacc tggccgcgct gatgcccgag accgacatgc tggcgcgcga ctggccgctg
     8281 cccgaaaaag cgcagtccga catcgacgac aagaagcgca agccgagcaa ggaaattcgt
     8341 gtcgacgcca tgcgcgaggc catcgagttc gcccagcgca ccagcgcgcg cgggcgcggc
     8401 aaggcggtgc tggtgtaccc ggccgagcgc atgaacgcca tcaccgccaa cgcgctgctc
     8461 aagacgctgg aagaaccggc cggtgacatg cgctttgtgc tggccaccga cgccgcccac
     8521 ctgctgctgc ccaccatccg cagccgctgc ctggcgcaca ccatggcgtg gccggacgag
     8581 gccgaagcct tggcctggct ggcgacgcag ggcgtgccgg ccgaggaggg cgccttgctg
     8641 ctgcgcgccg ccggcggccg gccggccgag gcgcttgcgc tggcgcaaac caagggcagc
     8701 gcgcaaagct gggcgcaact gccgcgcgca ctggcgcggg gcgacacggc ggcgctggcc
     8761 gatctgccgc tggccgaggc ggtgtcgcgc ctgcaaaagc tgtgtcacga cctgctggcg
     8821 ctgcaatcgg gcgccacgcc gcgctttttt gccgccgccg atttaccggc gcagccgctg
     8881 tcggccgccg cgctggggcg ctggtggcag caactgggcc gctcggcacg cacggccgaa
     8941 catcctttaa accccggttt ggcggcagaa ttcctcgtca gcagcgcgcg tcaggcccta
     9001 aactcgcggc gctgaaacct ttttgctgct gttgccatgt ccaccgcccc tgcacccgtc
     9061 gcttcgtcca cgccgcgccc cagcgtcatc cagctggcga tcaaggaaaa ggcggcgctg
     9121 tacgcggcct acatccccat gtttgccgaa ggcggcatct tcatcccgtc ggcgcgcgaa
     9181 catcaactgg gcgacgacgt gtacgtgctg atcacgctgc cggacgaccc gcagcgctac
     9241 ccggtggccg gcaaggtggc gtggatcacg cccgcaaggt cgaccggcaa ccgcacgcag
     9301 ggcgtgggca tccggtttcc gaaagacgag aaatcgcagc agctcaaggt caagatcgag
     9361 cagattctgg gcgcggcgct cggctcggac cggccgacgc agaccatctg aacgcatcta
     9421 acctcgcgtg cccatgacgc ggcgcgcatc gacagcgcgg cctggcgcgc gctgctagca
     9481 ttggtggcag cttgtttctc tgacgcgcgg ttgcgctctg catgttcacc gattcccact
     9541 gccacctgac ttttcccgaa ctggcgggcg acatgcccgg cgtgcgcgcc gccatggccg
     9601 ccgcccgggt gacgcgcgcg ctgtgcatca gcaccacgat ggaagagttt ccgtccgtgc
     9661 aggcgctggc gaccgagtac gacaacttct gggccagcgc cggcgtgcac cccgacagcg
     9721 aggacgtgca cgaacccagc gtggccgaac tggtggccgc gacgcaaatg cccaaggtgg
     9781 tggccatcgg cgaaacgggc ctcgactact accagatgga agagcgcaag ggcggccgcg
     9841 gcatcgccga catggaatgg caacgcgagc gctttcgcac ccacatccgc gccgcgcggc
     9901 aggtgggcgt gccgttggtt atccacaccc gcgcggcgtc cgacgacacg ctggcgatcc
     9961 tgcgcgaaga gggcgagcag ggcggcgcgg gcagcgctgg gggcgtgttt cattgcttta
    10021 ccgagacgcg cgaagtggcg cgcgccgcgc tcgatctggg cttttacgtg tcgctgtcgg
    10081 gcatcgtcac cttcaagaat gcgcgcgaat tgcacgaagt cgccacgctg attccgtgcg
    10141 accggttgct gatcgaaacc gattcgcctt acctggcgcc ggtgccgtac cgcggcaaga
    10201 tcaacacgcc ggcctatgtg ccgcacgtgg cggcgcgcct ggccgagctg cgcggcacca
    10261 gcgccgagca catcggcgag ctgaccagtg ccaatttcac ccgtctgttc aaaagggttg
    10321 cgtcatgacc aaatttcttc aagccttgct ggccgctgcg ctggcgctgt tggcgtccgt
    10381 tgcggtggcc gatgaccgcg gcgccttcga gcgcgccatc aagcaggaca acgacagcgc
    10441 cgtgcgcagc atgctgtcgc gcggcttcga tcccaacacg cgaaacgaaa aaggcgcgcc
    10501 cggcctgtac atggcgctgc aggagggttc gctgaaagtg gcgcgcgtgc tgatcgactc
    10561 gccgcgcctg aagcccgagg accgcaacgc cagcgatgaa agcccgctga tgatggccgc
    10621 gctcaagggc gagcttgacc tggccaagcg cctgattgcg aagggcgccg acgtcaacaa
    10681 gcccggctgg acgcccctgc actacgccgc caccagcggc catctggaca tgatccgcct
    10741 gctgctggaa gaacacgctt tcatcgacgc gcagtcgccc aacggcacca cgccgctgat
    10801 gatggcggcg tcctacggat cgccggaagc ggtcaagctg cttgttgaag aaggcgccga
    10861 catcgccatg cgcaaccaga agcagatgac cgccatggat ttcgcccgtc gcgccgagcg
    10921 ccgcgacgcg gtcgaattgc tgacacaggc cgagcgctac aaggcgcaat cgcgtccgcc
    10981 ggccgaacgt gggcgttggt gagtcggcag ccggagcctt cgcatcagtc ggtggccaca
    11041 ccgcgtcgtg ggcggccggc gctgtcaggc ggcctctctt gggttggccg ttgggccaga
    11101 acgcggctcg catgaactgg cgtgccggcc gcaatccttc caaaggtggt gcaaaagcgg
    11161 cgccgtcttt ggcacccagc agccatctgc gcagcgccgg gctgtcgatg aggtggcaat
    11221 ctggctgcga ccagacacag atgatttcat gccgctcggc gggtgtcagc cgattgacct
    11281 ggttcagcag gagccgcgca cgtcgcagcc atgacctgcg gacaggcaaa ctcgggcggc
    11341 cagtgggcaa acgccagacg atgaagttca gattgttggt tgctggcgtc agcgcgcgtc
    11401 ctcgcaacga aagtctcctt gatggttggt tgccaagctc gctggggtcg gcccgacgat
    11461 cgcattggga tgatccatga cttcatacga tgtgcattct tccgacttgt caagtgaaat
    11521 cttgtaaagt tata
//
LOCUS       DAOMVO010000042        11423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1338_length_11423_cov_12.623505, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000042 DAOMVO010000000
VERSION     DAOMVO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1338_length_11423_cov_12.623505"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKV30_03685"
     CDS             <1..562
                     /locus_tag="PKV30_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL29356.1"
                     /translation="AAANFPGLKITRSAFNPATPDYQKSMTELAAAQPTVLLLLANSA
                     HSTQFLTTWKSKASPPFVLNLAGQANGQFASGMKGYDGAAAFATVTPSPWETKIDAQR
                     EYPRIAKSAGMAPSYLGFESYLNARALIEAVTQTKSPTKTELVRWLDNAPRLDLGGYS
                     VGYGGDKLVSNFTDLALLRSDGTYRH"
     gene            584..2389
                     /gene="uraD"
                     /locus_tag="PKV30_03690"
     CDS             584..2389
                     /gene="uraD"
                     /locus_tag="PKV30_03690"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HRL29357.1"
                     /translation="MALSLDELNRASPEQAAAMLDGLYEHTPWIAAEALNERPFKSLA
                     HLKHVMAQVLARATVDQQLALIRAHPELAGKAMVAKTLTAESTHEQGKAGLTECTPDE
                     FARIQKLNADYNAKFGFPFILAVRGPRGAGLTKQQIIDTFARRLANHPDFERAEALRN
                     IHRIAEIRLADKFGAEPVLGNDVWDWHEKLSLHSDPGFAENGQLTVTYLTDAHRAAAA
                     FIAQTMREAGFDEVGIDAVGNVVGRYFSDSASRMSGKRQSPKTLMTGSHYDTVRNGGK
                     YDGRLGIFVPIACVRELHRAGRRLPFDFEVIGFAEEEGQRYKATFLGSGALVGQFNPD
                     WLAQADADGVTMREAMQHAGLNVDDIAKLQRNPKDYLGFIEVHIEQGPVLNELNMPLG
                     IVTSINGSIRFVGEFIGQASHAGTTPMGRRRDAACAAAELALYIEERAAEDGDSVATM
                     GLLGVPSGSINVVPGRCAFSLDLRAPNDAQRDALAADVLEKLQQIAMRRGVTYTLEET
                     MRASAAPSDPAWQKRWEAAVESLGLPVFRMPSGAGHDAMKLHEVMPQAMLFVRGENAG
                     ISHNPLESTNAEDMQLAVQAMQRLLQDLTHESLQQ"
     gene            2444..3691
                     /locus_tag="PKV30_03695"
     CDS             2444..3691
                     /locus_tag="PKV30_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRL29358.1"
                     /translation="MNPTYAALDQWIDQHFDEEVAFVQALVRVPTDTPPGNNAPHAEK
                     AAELLQSVGFDAEKHTVPTAEVQAYGMESITNLIVRRRFGDGGLTIALNAHGDVVPPG
                     EGWSHDPYGAVIENGQMYGRATAVSKGDFGTFTFALRALEAVAKPKHGAVELHFTYDE
                     EFGGELGPGWLLEKGLTKPDLMIAAGFSYEVVTAHNGCLQMEVTVHGKMAHAAVPATG
                     IDALQGAVAIMNQLYAENRRYQGITSRVAGITHPYLNIGRVEGGTNTNVIPGKVVFKL
                     DRRMIPEENPADVEALIRQVIADAVDGFNAGRPDDGIHVDIKRLLLAKAMTPLSGNAP
                     LVEAIQKHGEAVFGDQPAAVGTPLYTDVRLYVERGIPGVIYGAGPRTVLESHAKRADE
                     RLELEDLRGATKVVARTLADLLG"
     gene            3781..4743
                     /locus_tag="PKV30_03700"
                     /pseudo
     CDS             3781..4743
                     /locus_tag="PKV30_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803717.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            complement(4865..5077)
                     /locus_tag="PKV30_03705"
     CDS             complement(4865..5077)
                     /locus_tag="PKV30_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRL29359.1"
                     /translation="MSVAKVIEISASSTKSFEDAILTGVARATDSIDQVKGAWIKEQK
                     VVIEGGKIKAYRVSMNVTFVVGQVDK"
     gene            5299..7104
                     /locus_tag="PKV30_03710"
     CDS             5299..7104
                     /locus_tag="PKV30_03710"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRL29360.1"
                     /translation="MYQYTDFDRQFVTLRAQQFRDQLERWQRGELTDEQLLPLRLQNG
                     WYIQRYAPMARIAVPYGEISSTQLRMLARIARDYDRPDPELLAHAQQTQDELQASQPG
                     LTLAAPPLKFGYGHFTTRTNVQFNWIPITKAADVMDLLASVHMHGIQTSGNAIRNITC
                     EALAGIAADEIVDTRPFAEITRQWSTLHPEFAFLPRKFKIAFNGAEEDRAATGWYDIG
                     IQALRNDAGDVGFTVKVGGGMGRTPVIGSIVREFLPWAELLNYIEAVVRVYNRYGRRD
                     NKWKARIKILVKAEGQKFIDEVEAEYQAIVQQDGAPHTITQAELDRVTANFVVPPLRP
                     AGVPSEVSITAKPFQRWLTQNVGKHRLPDLCVVTLSFKRPGWAPGDADADTLDALAEL
                     ADQFSAGEARLTHEQNLLLPWVHSGDLPALYERAKALGLVQPNIGLLTDLIACPGGDF
                     CSLANARAIPIAAALTELYQDIDEVTDLGPIDLHISGCINSCGHHHSGHIGILGVDKD
                     GKEWYQVTLGGSDGSALSGPATPGKVVGPSFSAAEVPAVVEAILDTYRELRTSAAERF
                     IDTLRRVGHDPFKQAANGARHTDHRAARQAEEATA"
     gene            7123..7533
                     /locus_tag="PKV30_03715"
     CDS             7123..7533
                     /locus_tag="PKV30_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HRL29361.1"
                     /translation="MKIIAASASPTSANAPKTLKVANDADLAALAAEGALNGIERVEL
                     DFPKFTDGRAFSQAVLLRRRFGFGGDIRATGDVLIDQLVQMARSGFTSAVLAPGVDAA
                     AAERQFARYSAFYQGDALQPRPHFARNDGEREAA"
     gene            7548..8423
                     /locus_tag="PKV30_03720"
     CDS             7548..8423
                     /locus_tag="PKV30_03720"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRL29362.1"
                     /translation="MLGGDAPTASAIDLYAKPSPDFSTKLHETIVTLLKAASAGPVAQ
                     ANSLGAEDMVITHLINAHQLPIGVFVLDTGALHAESLALLERLQAQQAGYAHAPLTVC
                     RPVAEAVVQFVAREGQDAMYKSIELRKACCGIRKLEPLARALAGQAAWVTGLRKEQSG
                     ARADVALVDRSEEQSKGLLKYNPLAHWSWGDVWHYIAQNAVDYNPLHDQFYPSIGCEP
                     CTRAITLGEDFRAGRWWWEDEAAKECGLHVKDSNSLPLWRRVGVGANHLPDDPSGPHP
                     HLPPAVEGASQKAHA"
     gene            8420..9364
                     /gene="cysD"
                     /locus_tag="PKV30_03725"
     CDS             8420..9364
                     /gene="cysD"
                     /locus_tag="PKV30_03725"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRL29363.1"
                     /translation="MNARTHIDLLQPAAHHLSNAHLDALEEETIFILREVAAAFERPA
                     LLFSGGKDSLVMLRCAEKAFGAGRIPYPLLMIDTGHNFPEVTDFRDQRAAELKADLIV
                     RSVEDSMARGTVRLAHPGESRNVHQSVTLLEAIEEFRFDALIGGARRDEEKARAKERI
                     FSHRDSFGQWQPKSQRPELWTLFNTRLAPGEHFRVFPISNWTELDVWQYIEREQIALP
                     SIYYAHPREVVERRGLLVPVTPLTPPKDGEAVETRTVRFRTVGDITCTCPVESDAATP
                     GQIVIETLAADVSERGATRMDDKTSEASMEKRKKDGYF"
     gene            9500..10894
                     /locus_tag="PKV30_03730"
     CDS             9500..10894
                     /locus_tag="PKV30_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL29364.1"
                     /translation="MTTTTSIAARAYPTSARDLKNTESALRFITCGSVDDGKSTLIGR
                     LLVDSKAVLQDHLAGVTRGGEADLALLTDGLQAEREQGITIDVAYRYFNTEARKFIIG
                     DAPGHEQYTRNMVTAASQADAAVVLVDATKLDWADAGLALLAQTRRHSLLLNLLRVPS
                     VVFAINKLDAVAEPEQAYLHIRGALQAFAREAGIEPAAIVPVSALKGWNVVEAGQAQP
                     GWCGYVGPTLLEILERLPVTPREAHLPLALPVQWVEKPTAELQAAQSAEAQGRRVFWG
                     RVATGSVAPGMPVQLLPGGQNAVVAQVLDHARQPGRIGAGHSAGIVLDREVDVSRGDW
                     IVAARKPHAVVAADDFDDDALPQAVWPTTRELSATIAWLDDEPLVAGRVYWALHGHRW
                     VKAKVKRVVERLNIHTLARAEAAQLPPNAIGHVELALQELIPATAFAHSRVLGALILV
                     DTASHKTAGAVLVR"
     gene            11051..11380
                     /locus_tag="PKV30_03735"
     CDS             11051..11380
                     /locus_tag="PKV30_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRL29365.1"
                     /translation="MTHVVTEACINCKYTDCVDVCPVDCFKEGPNFLTIDPDECIDCA
                     VCIPECPVNAIYAEEDLPKDQLHMIQINAELARSPNYKTLTKRKDPLPDHEAWAQRTD
                     KLPELKR"
BASE COUNT         2116 a         3954 c         3546 g         1807 t
ORIGIN      
        1 ggccgccgca aacttcccag ggctcaagat cacgcgcagt gcgttcaatc ccgcaacgcc
       61 cgactaccag aagtcgatga ccgaactggc agctgcgcag cctacggttc tgctgttgct
      121 cgccaactcg gcgcattcga cacagttcct gaccacctgg aaatcgaagg catcgccgcc
      181 cttcgtcctc aacctcgcgg gccaagccaa tgggcagttt gcctctggca tgaagggcta
      241 cgacggcgcc gccgctttcg ccaccgtcac gcccagcccg tgggagacca aaatcgacgc
      301 ccagcgcgaa tacccgcgca tcgccaaatc ggcgggcatg gcgccgtcgt acctgggttt
      361 cgagagctac ctgaacgccc gcgccttgat cgaggcggtg acgcagacca aatcgcccac
      421 caagaccgag ctggtgcgct ggctcgacaa cgcgccgcgc ctcgacctcg gcggctattc
      481 ggtgggctac ggcggtgaca agctggtctc caacttcacc gacctggcgc tgctgcgctc
      541 ggacggcacc taccgccact gatcttttct cgggaacaac cgcatggccc tgtcgctcga
      601 cgaactcaat cgcgcatcgc ctgagcaggc ggcggccatg ctggatggct tgtacgaaca
      661 cacgccgtgg atcgctgccg aggccttgaa cgagcggccc ttcaaatcgc tcgcgcattt
      721 gaagcacgtg atggcgcagg ttctggcgcg cgccacggtg gaccagcaac tggcgctgat
      781 ccgcgcccac cccgagctgg cgggcaaggc gatggtggcc aagacgctca ccgccgaatc
      841 gacccacgag caaggcaagg cgggcctcac cgaatgcacg ccggatgaat tcgcacgcat
      901 ccagaagctg aacgccgact acaacgccaa gttcggcttt ccgttcatcc tggccgtgcg
      961 cggcccgcga ggcgcgggat taacgaagca gcagatcatc gacacctttg cgcggcggct
     1021 cgccaaccac ccggatttcg agcgcgccga ggcgctgcgc aacatccacc gcattgccga
     1081 aattcgcctg gccgacaagt tcggcgccga gccggtgctg ggcaacgacg tgtgggactg
     1141 gcatgaaaag ctgtcgctgc acagcgaccc cggctttgcc gaaaacggcc agctcaccgt
     1201 cacctacctg accgacgcgc accgcgccgc agcggccttc atcgcgcaga cgatgcgcga
     1261 ggcgggcttt gacgaggtcg gcatcgacgc cgtcggcaac gtcgtaggtc gctatttttc
     1321 agatagcgcc tcgcgtatgt ctggcaagcg ccagagcccc aaaacactca tgaccggcag
     1381 ccactacgac actgtgcgca acggcggtaa atacgacggg cgcctgggca tcttcgtgcc
     1441 cattgcctgc gtgcgcgagc tgcaccgcgc ggggcgccgt ctgccgttcg atttcgaggt
     1501 gatcggcttt gccgaagaag aaggccagcg gtacaaggcc acttttctcg gctctggcgc
     1561 gctggtcggg cagttcaatc ccgactggct ggcgcaggcc gacgccgatg gcgtgaccat
     1621 gcgcgaggcg atgcagcacg cgggcctgaa cgtggacgac atcgccaagc tgcagcgcaa
     1681 ccccaaggac tacctcggct tcatcgaagt ccatatcgag caaggcccgg tgttgaacga
     1741 gctgaacatg ccgctgggca tcgtcacttc gatcaacggc agcatccgct ttgtcggcga
     1801 attcatcggc caggccagcc acgccggcac cacgccgatg ggccggcgcc gcgacgccgc
     1861 ctgtgccgcg gccgagctgg cgctctacat cgaggagcgc gcggccgaag acggtgattc
     1921 ggttgccacc atgggcctgc tgggcgtgcc cagcggctcg atcaacgtgg tgcctggccg
     1981 ctgcgccttc agcctcgatt tgcgcgcgcc gaacgacgcg cagcgcgacg ccctggccgc
     2041 cgacgtgctt gaaaaactgc agcagatcgc catgcgccgc ggggtgacct acacgctgga
     2101 agaaaccatg cgcgccagtg ccgcgccgag cgacccggcg tggcaaaagc gctgggaagc
     2161 agccgtcgaa tcgctgggcc tgcccgtgtt ccgcatgccc tcaggcgccg ggcatgacgc
     2221 catgaagctg cacgaggtga tgccgcaggc catgctgttc gtgcgcggcg aaaacgccgg
     2281 catcagccac aacccgctgg aatcgaccaa cgccgaggac atgcaactgg ccgtgcaggc
     2341 catgcagcgg ctgcttcaag acctgacgca cgagtcgcta caacaataag agctgcttgc
     2401 gctggccaga caagcgctgg cgccactttt taatcaaaac atcatgaacc cgacctacgc
     2461 cgctcttgac cagtggatcg accagcactt cgacgaggaa gtcgcctttg tgcaagcgct
     2521 ggtgcgcgtg cccaccgaca cgccgcccgg caacaacgca ccgcatgccg agaaggccgc
     2581 cgagttgctt caaagcgtgg gtttcgacgc cgaaaagcac accgtgccca ccgccgaggt
     2641 gcaggcgtac ggcatggaaa gcatcaccaa cctgatcgtg cgccgccgct ttggcgatgg
     2701 cggcctgacg attgcgctga acgcccacgg cgacgtggtg ccgccgggcg aaggctggtc
     2761 gcacgacccg tacggcgctg tgattgaaaa cggccagatg tacggccgcg ccaccgccgt
     2821 cagcaagggt gatttcggca ccttcacctt cgcgttgcgc gcgcttgaag ccgtggccaa
     2881 gcccaagcat ggcgcggtcg agctgcactt cacctacgac gaagaattcg gcggcgaact
     2941 cggccccggc tggctgctgg aaaaagggct gacaaaaccc gacctgatga tcgccgccgg
     3001 cttcagctac gaagtggtga ccgcgcacaa cggctgcctg cagatggaag tgacggtgca
     3061 cggcaagatg gcgcacgccg ccgtgcccgc caccggcatc gacgcgctac agggcgccgt
     3121 cgccatcatg aaccagctgt acgccgagaa ccggcgctac cagggcatta cctcgcgcgt
     3181 ggcgggcatc acgcacccgt acctgaacat cggacgcgtc gaaggcggca ccaacaccaa
     3241 cgtgattccg ggcaaggtgg tgttcaagct ggatcgccgc atgatccccg aggaaaaccc
     3301 ggcggatgtc gaggcgctga tccgccaggt gatcgccgac gcggtcgatg gcttcaacgc
     3361 cggccggccc gacgatggca tccacgtcga catcaagcgc ctgctgctgg ccaaggccat
     3421 gacgccgctg tccggcaacg cacccctggt cgaagccatc cagaagcacg gcgaagcggt
     3481 gttcggcgac cagcccgccg ccgtcggcac ccccctctac accgatgtgc gcctgtacgt
     3541 cgagcgcggc atccccggcg tgatctacgg cgccggcccg cgcaccgtgc tggaaagcca
     3601 cgccaagcgc gccgacgagc gccttgagct ggaagacctg cgcggcgcca ccaaggtggt
     3661 ggcgcgcacg ctggccgacc tgctcggctg accttttttc atcaaccacc agaggcgcaa
     3721 agcccgctct tttttgttca tcgaggagaa gccatcacat ctgccacgac ccatccggtc
     3781 cctgaacagg tggccatgct gatctcggcc gacctgtttt gctgcggcct ggtcacgctg
     3841 atccagtcgc tcggcggcac gcagtagttc ggcaacaagc tgccggtgat gatgggcgtg
     3901 acctttgccg ctgtggcgcc catggtggcc atggccaatg ccaacccggg cgtggcgggt
     3961 gcgggggcga tttttggcgc catcatcggc gcaggtgtca tatcgatagc catcgcgccc
     4021 atggtcagcc gcatgctgcg gttttttcca tcagtggtca ccggcaccat catcctgatc
     4081 atcggcatca gcctgatgcg ggccggcatc aactggatct tcggcaaccc ggtcggcccc
     4141 acggcgccca gcgtggtcaa cctcgaatac tgcaagtggc tggaagaggc cgccaagacc
     4201 gctggcgcgc cgggttcggc cattccggcc gtgcccaagg gcatggcaat tgcgcccaac
     4261 gtgcccaacc ccaagtatgc cgacctgacg ggcgtgggca tcgccgcgct ggtgctggtg
     4321 tcgatcctgc tcatcacgcg ctttgccaag ggcttcgtgg ccggcggcgt ggtgctgctg
     4381 ctgcttggct tgctgccgaa aatggcggcg ctggtcgaat cgctgccgac catggtgctg
     4441 ggccgcgcgg gcctggtgat gttcggtatg gtggcggcca ccggcattcg catcctggcc
     4501 ggcgtcgact tcaagcacaa ccgcttcaac gccatgatcg tcgccatctc ggtcggcatc
     4561 ggcatgattc cgctgatcgc gcccaacttc aagcaatgga tggcgcatgg cgttcacccg
     4621 ctgatcgaat cgggcatcct gctgtcgtcc atcgccgcgg tgctgctgaa cctgttctac
     4681 aacggtgcgc gcgaagacac gaccgccacc atcgccgcgg ccaagcaggc tgaggcgcac
     4741 tgatatcctg atttactatt tatttggtag ctgctcgcgc ttgccccgcc tgcgccagag
     4801 ccacttttaa acaagaaagc cgacccatgg gtcggctttt ttttctgcac agacgcgcca
     4861 tcgattactt gtccacctgc cccaccacaa acgtcacgtt catgctcacg cggtaagcct
     4921 tgatcttgcc gccctcgatg acgaccttct gctccttgat ccaggcgcct ttgacctgat
     4981 cgatgctgtc ggtggcgcgc gcaacgccgg tgaggatggc gtcctcgaag ctcttggtgc
     5041 tggatgcgct gatttcgatg accttggcaa cggacatggg ctttctcctt ggggttgggg
     5101 agccggatgc ggcgcggtgg gaacgaaccc gcaccataag tcgcccccgc cgcgtccgct
     5161 gtgggcattt gtctgacgcg cccgtcggtg cctgcccccg tttgcttatt cctgatcggc
     5221 atatatcaac aacaaactat tggtttgttt tggcataaag gctcgctaca ttgccggctg
     5281 acttgaatcc agcccgcgat gtaccagtac accgatttcg accgccagtt cgtcaccctg
     5341 cgcgcccagc aattccgcga ccagctcgag cgctggcagc gcggcgagct gaccgacgag
     5401 caactgctgc ccctgcgcct gcaaaacggc tggtacatcc agcgctacgc ccccatggcg
     5461 cgcatcgccg tgccgtatgg cgaaatcagc agcacgcaac tgcgcatgct ggcgcgcatc
     5521 gcgcgcgact acgaccggcc cgaccccgaa ttgctggccc acgcgcagca aacgcaagac
     5581 gagctgcaag cctcgcagcc cggtttgacg ctggccgcgc cgccgctgaa gttcggctac
     5641 ggccacttca ccacgcgcac caacgtgcag ttcaactgga tccccatcac caaggcggcc
     5701 gacgtgatgg acctgctggc cagcgtgcac atgcatggca tccagaccag cggcaacgcc
     5761 atccgcaaca tcacctgcga agcgctggcc ggcatcgcgg cagacgagat cgtggacacg
     5821 cgcccgtttg cggagatcac gcgccagtgg agcacgctgc accccgaatt cgcctttttg
     5881 ccgcgcaagt tcaagattgc cttcaacggc gccgaggaag accgcgccgc caccggctgg
     5941 tacgacatcg gcatccaggc gctgcgcaac gacgcaggcg acgtcggctt caccgtcaag
     6001 gtgggcggcg gcatgggccg cacgccggtc atcggcagca tcgtgcgtga atttctgccc
     6061 tgggccgaac tgctcaacta catcgaagcc gtcgtgcgcg tctacaaccg ctacggccgg
     6121 cgcgacaaca agtggaaggc gcgcatcaag attctggtca aagccgaagg gcagaagttc
     6181 atcgacgagg tcgaggccga ataccaggcc atcgtgcagc aagacggcgc accgcacacc
     6241 atcacgcaag ccgagctgga ccgcgtgacc gccaacttcg tcgtgccgcc gctgaggccc
     6301 gccggcgtgc ccagcgaagt cagcatcacc gccaagccgt tccagcgctg gctgacgcag
     6361 aacgtgggca agcaccgcct gcccgacctc tgcgtcgtca ccctctcctt caagcgcccc
     6421 ggctgggcgc cgggcgatgc cgacgccgac acgctggatg ctctggccga actggccgat
     6481 cagttcagcg ccggcgaagc acgcctgacg cacgagcaga acctgctgct gccctgggtc
     6541 cactccggcg acctgcccgc gctgtacgag cgcgccaagg cgcttggcct ggtgcagccc
     6601 aacatcggcc tgctcacgga cctgatcgcc tgcccggggg gcgacttctg ctcgctggcc
     6661 aacgcgcgcg ccatcccgat tgccgccgcg ctgaccgagc tgtaccagga catcgacgag
     6721 gtgaccgatc tgggccccat cgacctgcac atcagcggct gcatcaactc gtgcggccac
     6781 caccacagcg gccacatcgg catcctgggc gtcgacaagg acggcaagga gtggtatcag
     6841 gtcacgctgg gcggcagcga cggctcggcc ctgtcggggc ccgccacgcc gggcaaggtg
     6901 gttggcccgt cgttttccgc cgccgaagtg ccggccgtgg ttgaggcgat tctggacacc
     6961 tatcgcgagc tgcgcacaag cgctgccgag cgcttcatcg acacgctgcg ccgcgtcggt
     7021 cacgacccct tcaagcaggc ggccaacggc gcgcgccaca ccgatcaccg tgccgcacgc
     7081 caggccgaag aagccaccgc ctgaatccag cgaaagataa ctatgaaaat aatagctgca
     7141 agcgcaagcc ccacaagcgc caacgcccca aaaaccttaa aagtcgccaa tgacgccgac
     7201 ctggccgcgc tggcggccga gggtgcactg aacggcatcg agcgggtcga gttggacttt
     7261 cccaaattca ccgatggccg cgctttcagc caggccgtac tgctgcgccg ccgctttggc
     7321 tttggcggcg acatccgcgc cacgggcgac gtgctgatcg accaactggt tcaaatggcg
     7381 cgcagcggct tcaccagcgc cgtgctggca cccggcgtgg atgcggccgc ggccgagcgc
     7441 cagtttgcgc gctacagcgc cttctaccag ggcgatgcgc tgcagccgcg cccgcacttc
     7501 gcccgcaacg acggcgagcg ggaggccgcg tgagcgccgc cgtcagcctg ctcggcggcg
     7561 acgcgcccac ggcgtcggcc atcgatctct acgccaagcc ctcgcccgac ttttcgacca
     7621 agctgcacga gaccatcgtc acgctgctca aggccgcatc cgccggcccg gtggcacagg
     7681 ccaacagcct gggcgccgag gacatggtca tcacgcacct gatcaacgcg caccagctgc
     7741 ccatcggcgt cttcgtgctc gacaccggcg cgctgcacgc ggagagcctg gcgctgctcg
     7801 aacgcctgca agcccagcag gccgggtatg cgcacgcgcc gctcaccgtg tgccgcccgg
     7861 tcgccgaagc ggtggtgcag ttcgtggcac gcgaaggtca ggatgcaatg tacaagagca
     7921 tcgaactgcg caaggcctgc tgcggcatcc gcaagctgga gccgctggcg cgcgcgctgg
     7981 ctggccaggc cgcctgggtc accggtttgc gcaaagagca atcgggcgcc cgcgcagacg
     8041 tggccttggt cgaccgctct gaagaacaga gcaaagggct gctcaagtac aacccgctgg
     8101 cgcactggag ctggggcgac gtgtggcact acatcgcgca gaacgcggtc gactacaacc
     8161 cgctgcacga tcagttctac cccagcatcg gctgtgagcc ctgcacccgc gccatcacgc
     8221 tgggcgaaga ctttcgcgcc gggcgctggt ggtgggaaga cgaggccgcc aaggaatgcg
     8281 gcctgcatgt gaaggattcc aactccctcc ccctttggag gagggttggt gtgggggcca
     8341 accacctccc cgacgatccg agcggccccc atccccacct tcccccagcg gtggaaggag
     8401 ccagtcagaa agcccacgca tgaacgcgcg cacccatatc gatctgctgc aacccgcagc
     8461 gcaccacctg tccaacgccc acctcgacgc gctggaagaa gaaaccatct tcatcctgcg
     8521 cgaagtggcc gccgcgttcg agcgccccgc gctgctgttt tcaggcggca aggattcgct
     8581 ggtgatgctg cgctgcgcag agaaagcctt tggcgcgggc cgcattccgt acccgctgct
     8641 gatgatcgac actggccaca acttccccga agtgaccgat tttcgcgacc agcgtgctgc
     8701 agagttgaaa gcagatctga tcgtgcgcag tgtcgaagac agcatggcgc gcggcactgt
     8761 gcggctggcc cacccgggcg aatcgcgcaa cgtgcaccag tcggtcacgt tgctggaagc
     8821 catcgaggaa ttccgcttcg acgccctgat cggcggcgcg cggcgcgacg aagaaaaggc
     8881 gcgcgccaag gaacgcatct tcagtcaccg cgacagcttt ggccagtggc aacccaagtc
     8941 tcagcggccc gagctgtgga cgctgttcaa cacgcgcctg gcccccggcg agcatttccg
     9001 cgtgttcccg atcagcaact ggaccgaact ggacgtgtgg cagtacatcg agcgcgagca
     9061 gatcgccctg ccgtcgattt actacgcgca cccgcgcgaa gtggtggagc gccgtggcct
     9121 gctggtgccc gtgaccccgc tcacgccgcc gaaggatggc gaggcggtgg agacccgcac
     9181 cgtgcgtttt cgcaccgtgg gcgacatcac ctgcacctgc ccggttgaaa gcgacgccgc
     9241 aacgcccggc cagatcgtca tcgaaaccct ggccgccgac gtgagcgagc gcggggcgac
     9301 gcgcatggac gacaagacgt ccgaggcttc gatggagaag cgcaagaagg atgggtactt
     9361 ctaaaaaccc aacatccgga cgcgaaagac gcaaaggttc cgcgaggacg cgaaagaaaa
     9421 cctttttgaa cttgtctttt tcgcgttctt cgcgtgatct tcgcgtcctt cgcgtccggc
     9481 tgttcgagaa agcttcacca tgaccactac aacatctata gctgcccgcg cttaccccac
     9541 aagcgccaga gacctaaaaa acaccgaatc cgcgctgcgc ttcatcacct gcggcagcgt
     9601 cgatgacggc aagagcacgc tcatcggccg cctgctggtc gacagcaagg cggtgctgca
     9661 agaccacctg gccggcgtca cgcgcggcgg cgaggcggac ctggcgctgc tcaccgacgg
     9721 cctgcaggcc gagcgtgagc aaggcatcac catcgacgtg gcctaccgct atttcaatac
     9781 cgaagcgcgc aagttcatca tcggcgacgc gccggggcac gagcagtaca cgcgcaacat
     9841 ggtcaccgcc gcctcgcagg ccgatgccgc cgtggtgctg gtcgatgcca ccaagctcga
     9901 ctgggccgat gccggcctgg cgctgctggc gcagacacgg cgccattcgc tgctattgaa
     9961 tctgttgcgc gtgccctcgg tggtgtttgc catcaacaag ctggacgcgg tggccgaacc
    10021 cgagcaggcc tacctgcaca ttcgcggcgc gctgcaggcg tttgcgcgcg aggcgggcat
    10081 cgagcccgcg gccatcgtgc cggtgtcggc gctcaagggc tggaacgtgg tggaagccgg
    10141 ccaagcccag cccggctggt gcggctacgt tgggccgacg ctgctggaga ttctggagcg
    10201 cctgcccgtg acgccgcgcg aagcgcacct gccacttgcc cttccggtgc aatgggtcga
    10261 aaaacccacg gccgagcttc aggccgcgca gagcgccgaa gcacaaggcc gccgcgtgtt
    10321 ctggggccgc gtggccaccg gcagcgtggc gccgggcatg ccggtgcaac tgctgcccgg
    10381 cggccagaac gcggtggtag cgcaggtgct cgatcacgcg cgccagccgg gccgcatcgg
    10441 cgccggccac agcgccggca tcgtgctcga ccgcgaagtc gacgtgtcgc gcggcgactg
    10501 gatcgtggcg gcgcgcaagc cgcacgccgt ggtcgcggcc gatgatttcg acgacgacgc
    10561 gctgccgcaa gccgtctggc cgaccacgcg cgagctgtcg gccaccatcg cctggctcga
    10621 cgacgagccg ctggtggccg ggcgcgtgta ctgggcgctg cacgggcacc gctgggtcaa
    10681 ggccaaggtc aagcgcgtgg tcgagcgcct gaacatccac accctggcgc gcgcggaagc
    10741 cgcgcaactg ccgcccaacg ccatcggcca cgtcgagctg gcgctgcaag agctgattcc
    10801 ggccaccgcc tttgcccaca gccgcgtgct gggcgcgctg atcctggtcg acacggccag
    10861 ccacaagacg gccggcgcgg tgctggtccg ctgaaggcac ccaccctaca aagaccccag
    10921 cgcgcgcaac ccttttgcgt tcaaggcttc agccgcccgc ccgcggttga gggcctaaaa
    10981 tcgctggttc accccgcgcc gtgctgcgcg ctttgcaaga tcactcgact cgctcgttcc
    11041 aagaaatccc atgacccacg ttgtcactga agcctgcatc aactgcaaat acaccgattg
    11101 cgtggacgtc tgccccgtcg attgcttcaa ggaaggcccg aactttctca ccatcgaccc
    11161 cgacgagtgc atcgactgtg ccgtctgcat ccccgagtgc ccggtcaacg ccatctacgc
    11221 cgaagaagac ctgcccaagg atcagcttca catgatccag atcaacgccg aattggcgcg
    11281 ctcgcccaac tacaagacgc tgaccaagcg caaggatccg ttgcccgacc acgaggcatg
    11341 ggcccagcgc accgacaagc tgccggaact gaagcgctga agcgccgcca ttgaaataaa
    11401 aaggcggttc tgcgcaggcc cca
//
LOCUS       DAOMVO010000043        11324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1358_length_11324_cov_13.331440, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000043 DAOMVO010000000
VERSION     DAOMVO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1358_length_11324_cov_13.331440"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKV30_03740"
     CDS             complement(<1..348)
                     /locus_tag="PKV30_03740"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRL29366.1"
                     /translation="MLAAIACAAFGLVPSAVAFALGALAMMALRIVGPDAAYRAVDWP
                     VVVLLGAMMPVDGAIESTGLARVIATGLIDTLAQGHAVLALTMILVVTMTLPDFMNNA
                     ATAAVMCPIALGAA"
     gene            complement(270..575)
                     /locus_tag="PKV30_03745"
     CDS             complement(270..575)
                     /locus_tag="PKV30_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRL29367.1"
                     /translation="MGWRLRYGVNLLAISREGGRSIQRLPSAPLAGGDVLLLQGRDGA
                     IASLAGDLGLVPLAERNLRIRNKREAIMPRRSCWRRLPARLSGWCRRPWLSRWARWP"
     gene            complement(1102..1287)
                     /locus_tag="PKV30_03750"
     CDS             complement(1102..1287)
                     /locus_tag="PKV30_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29368.1"
                     /translation="MSTHLARGLPLLVLLLAPVTVAGLAWLATLAQQLGRSLPDCNED
                     MGWPDGRSRLSENKPAM"
     gene            complement(1326..2267)
                     /locus_tag="PKV30_03755"
     CDS             complement(1326..2267)
                     /locus_tag="PKV30_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL29369.1"
                     /translation="MPPADFCIPTPADLPLAYPMTTPARRVGRFAETRSVRWLRRMFR
                     PLHRHAPAVAARLAYQLLTRPPRVAERPWQNALRQQAQHDWLPFGRGRLALYSWGSGP
                     TILLVHGWGARATHLGKMVPTLLAAGYRVVAFDAPGHGHSSGRVATLPQFAQAVAAVG
                     AHVGEVHTLIAHSFGVAMALWAQVDWGLQVQRQVLFSSLAHCKWVTEQFARLVELPEA
                     VLDRGRQLMVERSGGRMDWERLSVGELLRQTSTPTLALHDADDPEVPIEHLFSLITAC
                     AERPLQLHVTQGLGHHRLLGDRSVIERVVGFIEAPAY"
     gene            2404..2550
                     /locus_tag="PKV30_03760"
     CDS             2404..2550
                     /locus_tag="PKV30_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29370.1"
                     /translation="MTFLTLTAGAARPTLKPDLMARCPWRPWHWIDVTSSRHTHSSAR
                     RVSP"
     gene            2547..3269
                     /locus_tag="PKV30_03765"
     CDS             2547..3269
                     /locus_tag="PKV30_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29371.1"
                     /translation="MIRIRSHPPARSSATRHLRRLLCGGFLCGSALAALAGQGIAPGE
                     RQALLDLYARTGGEQWTVNTGWGGPVGSECHWYGVRCDDARQHVIGLHLPNNQLRGAL
                     PRLGGLPHLQTLTVSLNHLRGTLPLLAELPALRELKVNNNALVGPLPELRRLTQLERV
                     VLANNRFDGYLPPLAGLTQLREFDASNNQLSGLVPRLRGLERLRRFDVSFNRLQGVHE
                     ALPAVADVEGNPMTDVSDRVRL"
     gene            complement(3362..5080)
                     /locus_tag="PKV30_03770"
     CDS             complement(3362..5080)
                     /locus_tag="PKV30_03770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRL29372.1"
                     /translation="MSLLLPPPEHRGATQPVQLYILIRACELLGALLFLPVLPWWQPM
                     LWFTLVAGTGTWGFWRVRRPDFKLLPEARRRAIYRRYVWQSALAVGSAGFLLYVPGEH
                     GLHALLGLYMTMTASLVAMWGVRDLPRTGVAVALIIGPMALRFMFDGVRGPRPMLLVL
                     GLCGALLGVMIVYTAMVQARRMARESVLRQRAERATDAMAELSLSKSRFFAAVSHDLR
                     QPVHAIGLYLESLTRAMADQGDAEARRAVNGIRQSWRALDELLSQVLDLTRMDAGTLR
                     ASIGAVELAPLVRAVVMQHSAAAERAGVRVVALLGDERARHAFADELMLRRALSNLLD
                     NAIKFSQPGQRVLVALRPGAQCWQLQVRDAGPGIAADQHDGVFQEFVQLDNQARDRQQ
                     GYGLGLAISRRFARLMLGSLTLRSAPGAGSCFTLALPRASAPCAPVLDDAAQGPSSQL
                     PCDDIVFLPAARDILLVEDDPLVADAMCQLLGNWGQTVRHVNSVAEAWRQREFGQLAI
                     CDVRLPGGGNGLALALQLRALGKQVVLISGETDAALCTQAASAGLPLLTKPVSGAQMM
                     VLLQDA"
     gene            complement(5077..5784)
                     /locus_tag="PKV30_03775"
     CDS             complement(5077..5784)
                     /locus_tag="PKV30_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL29373.1"
                     /translation="MTNFTPATIAPSVLGRYAVAMTSVAAPLSGAGTAPTVILADDHD
                     LVRDGLKLMVCAMLPGAQVLEAGNAPALWQLARRVPTPQLALVDLNMPGMERGLRLAS
                     LAREQPRLPLIVVSALTSPDVVRRALDVPSVCAFVPKSAGVARMREAIEAALQGRRLD
                     PWLPDAAAAEALPDTGLTPRMQEIRALLRQGLSNKHIAQQLDLSEGTVKNYMSDIFRL
                     LSVSNRTQAAQYNPDTA"
     gene            complement(5908..7965)
                     /gene="fusA"
                     /locus_tag="PKV30_03780"
     CDS             complement(5908..7965)
                     /gene="fusA"
                     /locus_tag="PKV30_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRL29374.1"
                     /translation="MPSTTSATPAVGNIRTLALVGPAAAGKTTLAEALLQHTGAMGAA
                     GSVERGSTVSDHDALEIKAGHSLQSSVMHLDRGGCRIHLIDTPGAPDFIGQSLPALEA
                     VETAAIVINAAAGIEPMAQRMMAYAAERGLDRLVIVNRIDAQDVDLSAVLADIQAAFG
                     KECLPLNLPDAGATQVVDCFYNREGHCDFGAVADAHRALVEQVVEVDGDFVDRYLNDG
                     DIDANELHAPLEQALREGHLIPVCFVSARTGAGMPELLDVIARLLPNPTEGNPPDFLN
                     GEGADATLMHAKPDPAAHVLAHVFKVSIDPYVGKLGFVRVHQGSITPGTQLYVGNGRK
                     PFKVGHVYLQQGKNHVEVPSAGPGDICAVAKIEELHFNAVLHDAAEDDHIHLKPLPFP
                     VPVHGVAISPKRRGDEQRMWDIVGKLVAEDPCLKLEHLAQTNETVVYGLGELHLRMLL
                     ERLRDVHKFEVDTRPPRIAYRETITQNAAAQYRHKKQSGGAGQFGEVHLRIEPLPRGA
                     GFEFVDQVKGGTIPGQFMPAVEKGVREALAEGVIAGYPVHDVRVIVHDGKHHAVDSKE
                     IAFVTAGKRAFQAAIREAKPVVLEPIAQVQIAAPESAMGAITGDLSARRGMVSGTDSG
                     QLGQLTVNGQAPMAELADYQTRLNAMTAGQGRYSLALSHYEVVPPGVQQQLMGQHKVQ
                     DED"
     gene            complement(8059..9267)
                     /locus_tag="PKV30_03785"
     CDS             complement(8059..9267)
                     /locus_tag="PKV30_03785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRL29375.1"
                     /translation="MVGSPPPADKIVRKEDGSFYRFPQLRWTFSNARQLGATTNVSRG
                     LDSPSALPRAERADLAAVTFVPLGMTEPMTWGKAFDANYTDGVVVLHKGRIVYERYAG
                     VFKPDGQHLSQSLTKSFFGTIAAMLVADGQIDQNALVTQYVPELKGSAFADATVRQVM
                     DMRTGLKYSENYADPKAEIWMHARAGNVLPRPPGYQGPQSFYEFLQMVQKEGEHGGAF
                     AYKTVNSDVLGWVIRRITGKSAGQVLSERIWSRLGMEQDAYFTVDSVGTEFAGGGLNT
                     GLRDLARFGELMRCGGCWGGLFDGRQVIRQAVVDDIRRGGDRAAFTPAGYVSLPGWSY
                     RNMWWVTHNEHGAYMARGIHGQAIYVDPTAEMVIARYASHPLAANANNDPTSLPAYHA
                     LARHLMGATR"
     gene            9476..10087
                     /locus_tag="PKV30_03790"
     CDS             9476..10087
                     /locus_tag="PKV30_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRL29376.1"
                     /translation="MTESIATPQDVLAFWLGAWPIDQAAMQRVQAQWFSKNDAFDAEL
                     RQRFGATLAAARAGRLDDWARDADGWLALLIVLDQFSRNAFRGQPEGFAGDAQALAIA
                     QAGIARGDDQRVPPMARIFCYLPLEHAEDAALQARSVALFTALRDAPGAEPKAFFDGT
                     LDYAHKHQDVIARFGRFPHRNAILARASTAEEQAYLAQPGAGF"
     gene            complement(10147..10377)
                     /locus_tag="PKV30_03795"
     CDS             complement(10147..10377)
                     /locus_tag="PKV30_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29377.1"
                     /translation="MSVISFYIDQDTPGFRTTTGLLPTPDSDIPLCALSVGDTVSFPN
                     HRNRVYRVVSRHCNAGADMGEAEWLVRIEPVR"
     gene            complement(10555..>11324)
                     /locus_tag="PKV30_03800"
     CDS             complement(10555..>11324)
                     /locus_tag="PKV30_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRL29378.1"
                     /translation="VVAIKQQVQNRARQLIEEFMVATNGVTARFLAGKKRASIRRVVK
                     SPERWARIAEVAAERGWQLPPEPNSAALEQFLAAERKRDPLRFPDLSLIIVKLMGRGE
                     YVVERPGGPTIGHFGLAVRDYSHSTAPNRRYPDLITSRLIKAALSEAAAPYSHTELEF
                     LANRCTKQEDAANKVERQLRKSEAAMLLESHIGDEFDGVVTGSSNGNNWVRIFNPPAE
                     GKLLVARGRKINVGDKVRVKLRSTNVERGFIDFEMLG"
BASE COUNT         1766 a         3863 c         3915 g         1780 t
ORIGIN      
        1 cgctgcgccc agggcgatgg ggcacatcac ggccgcggtg gcggcgttgt tcatgaagtc
       61 cggcagcgtc atcgtcacca ccaggatcat cgtcagcgcc agcaccgcgt ggccctgcgc
      121 cagcgtgtcg atcagcccgg tggcgatgac gcgcgccagg ccggtcgact cgatcgcgcc
      181 gtccaccggc atcatggcgc ccagcagcac gaccaccggc cagtcgacgg cgcggtaggc
      241 cgcgtcgggg ccgacgatgc gcagcgccat catggccagc gcgcccagcg cgaaagccac
      301 ggccgacggc accagcccga aagccgcgca ggcaatcgcc gccagcatga gcgccgaggc
      361 atgatcgcct cgcgcttgtt gcggatgcgc aggttgcgct cggccagcgg caccaggccc
      421 aggtcgcccg ccagcgaggc gatggcaccg tcgcggccct gcagcagcag cacgtcgccg
      481 ccggccagcg gcgccgaggg caggcgctgg atcgagcggc cgccctcgcg cgagatcgcc
      541 agcaggttga cgccgtagcg caggcgccag cccatctggg tgacggtgct gccagggcgg
      601 ctggcatcag cagcgccagt gcgcccacat tgttcatgaa ggccgacagc agcgcgtcaa
      661 cgctggcgag cgccgccagc gtggtcagca gcccggcgtt gcgtggaatc aggtgatcca
      721 ccaacgcggc gacggcgccc gttttctcca gcgcggcgct catcaccagc acgccggcca
      781 ccgtgatcac ggccgggtgg ccgaagcctg caaacgccga ggccgtgggc accaagccca
      841 gcagcgtggt cgccagcagc gccgccagcg ccaccaggtc gtggcgaaag cgcccccaga
      901 tcaacaagcc gatcgtgacg atcagcacgg cgagactttt ccattggtcg ggcgtcgtgc
      961 ggtagtctga atcggtgcgg cgcgtcgggc gcagcaggtg ccatgctagc gcgcggtcga
     1021 gcgcgggccg atgcccggcc aactgccggc atgcctcgcc aggacgccct acgacttctc
     1081 gaccgccgcg ccgcagtcgc atcacatcgc cggcttgttc tcgctcaggc gtgaacgccc
     1141 gtccggccac cccatgtctt cgttgcagtc gggcaggctg cgccccagtt gctgcgcgag
     1201 cgtggccagc cacgccagtc cggcaacggt gacgggcgcc agcagcagca ccagcagcgg
     1261 cagtccgcgg gcgagatggg tcgacatgat gcgctctcct tggaaaacca ggacgacatg
     1321 aaagatcaat acgccggcgc ttcaatgaag ccgaccacgc gctcgatcac gctgcgatcg
     1381 cccagcagcc tgtggtgccc cagaccctgg gtcacatgca gttgcagcgg acgctcggcg
     1441 caggccgtga tcaggctgaa caggtgctcg atcggcacct ccgggtcgtc cgcatcgtgc
     1501 agcgccagcg ttggggtact ggtctgccgc aacagctcgc ccaccgacag acgctcccag
     1561 tccatgcgtc cgccggagcg ctcgaccatc agctggcggc cacggtccag caccgcttca
     1621 ggcaactcga ccaggcgcgc gaattgctcc gtgacccact tgcaatgggc gagcgaactg
     1681 aacagcacct gccgctgcac ctgaagaccc cagtcgactt gcgcccacag cgccatggcg
     1741 acgccgaatg aatgcgcgat cagcgtgtgc acctcgccga catgcgcgcc gacggcagcc
     1801 acggcctgcg cgaactgcgg cagcgtggca acgcggccgc tggagtggcc gtggccaggc
     1861 gcgtcgaacg ccaccacgcg gtagccggcg gccagcagcg tcggcaccat cttgcccaga
     1921 tgggtggcgc gcgcacccca gccgtgcacc agcaggatgg tcgggccgct gccccagctg
     1981 tacagcgcca accggccgcg accgaacggc agccagtcgt gttgcgcctg ctggcgcagc
     2041 gcgttctgcc aagggcgctc ggccacgcga ggcgggcgcg tcagcagctg ataggccagg
     2101 cgcgcggcca cggcgggggc atggcggtgc aatgggcgga acatgcggcg cagccagcgc
     2161 accgagcgtg tttccgcgaa gcggccgacg cggcgcgccg gcgtggtcat ggggtatgcc
     2221 agcggcaggt cggcaggcgt cggtatgcag aaatcggcgg gtggcatggt gttctccttg
     2281 cagtgggcgc ggcaccttgc cgcggggtga ggccacttta ggaaccgggt tggttcacgg
     2341 acaggtgaag ttggtcatgc gcgccgcggc agttcccccg cccgagggac gaagcggtgg
     2401 gacatgacct ttctcacgct gaccgccggc gccgcgcgcc ctacgctgaa gcccgatctg
     2461 atggcgcgct gcccgtggcg gccgtggcac tggatcgacg tcacttcgtc acgccacacc
     2521 cattcatctg caaggagagt ttcaccatga tcaggattcg ttcacatccc cccgcacgct
     2581 ccagcgccac ccgccacctg cggcgcctgc tgtgcggagg cttcttgtgc ggcagcgcgc
     2641 tggccgcgct cgcgggccaa ggcatcgcac caggcgagcg ccaggcgctg ctcgatctgt
     2701 acgcccgtac cggcggcgaa caatggacgg tgaacaccgg ctggggcggt cctgtgggca
     2761 gcgaatgcca ctggtacggc gtgcgctgcg acgacgcgcg gcagcacgtg atcggcctgc
     2821 acctgcccaa caaccagctg cgcggcgcgc tgccacggct cggcgggctg ccacacctgc
     2881 agacgctgac tgtgtcgctc aaccacctgc ggggcacctt gccgctgctg gccgaactgc
     2941 ccgccctgcg cgagctgaag gtcaacaaca atgcgctggt cggccccttg cccgaattgc
     3001 gccggctgac ccagctcgaa cgcgtggtgc tggccaacaa ccgcttcgat ggctacctgc
     3061 cgccgctcgc cggcctgacg cagttgcgcg aattcgatgc ctccaacaac cagttgtctg
     3121 gcctggtgcc gcgcttgcgc ggactggagc ggctgcgccg cttcgatgtc tcgttcaacc
     3181 gcttgcaggg cgtgcacgag gcgctgcccg ccgtcgccga cgtcgaaggc aacccgatga
     3241 ccgatgtgtc cgatcgggtg aggctctgaa ccagcggctg gtcaaatcat gatccgaata
     3301 ggtcgtcagc gcttgtccag caagcgcgag cagctatcct aaatgtagta ggcagcccga
     3361 ctcaggcgtc ctgcagcaac accatcatct gcgcgcccga caccggtttg gtcaacaagg
     3421 gcaggccggc gctggccgcc tgggtgcaca gggctgcatc ggtctcgccg ctgatcaaca
     3481 ccacctgttt gcccagcgcg cgcaattgca aggccagtgc cagcccattg ccgccgccgg
     3541 gcaggcgcac atcgcagatc gccagctggc cgaactcccg ctggcgccac gcctcggcca
     3601 cgctgttgac gtggcgcacg gtctggcccc agttgcccag cagctggcac atggcgtcgg
     3661 ccaccagcgg gtcatcctcc accagcagaa tgtcgcgcgc tgccggcagg aagacgatgt
     3721 cgtcgcaagg cagctgtgac gacgggccct gcgccgcgtc atccagtact ggggcgcacg
     3781 gcgcgctggc acggggcagt gcgagcgtga agcagctgcc ggcgccaggc gccgagcgca
     3841 gcgtcaggct gcccagcatc aggcgggcga agcggcgcga tatcgcaagg ccgaggccgt
     3901 agccctgctg gcggtcgcgc gcctggttgt cgagctgcac gaactcctgg aacacgccgt
     3961 cgtgctggtc ggctgcgatg cccgggccgg catcgcgcac ctgcaattgc cagcattgcg
     4021 cgcccgggcg cagcgccacc agcacgcgct ggccgggctg cgagaacttg atggcgttgt
     4081 ccagcaggtt cgacagcgca cgccgcagca tcaattcgtc ggcaaaggcg tggcgcgcgc
     4141 gctcgtcgcc cagcagcgcg accacgcgca cccccgcgcg ctcggccgcc gcgctgtgct
     4201 gcatcaccac cgcccgcacc agtggcgcca gttcgaccgc cccgatgctg gcgcgcagcg
     4261 tgccggcgtc catgcgcgtc agatccagca cctgggacag caactcgtca agcgcccgcc
     4321 acgattggcg gatgccgttg acggcgcggc gcgcctcggc atcgccctgg tccgccatcg
     4381 cgcgcgtcag cgattcgagg tacaggccga tggcatgcac cggctggcgc aggtcgtggc
     4441 tgacggcggc aaagaagcgc gatttcgaca ggctcagctc ggccatggcg tcggtggcgc
     4501 gctcggcgcg ctggcgcagc accgattcgc gcgccatgcg ccgcgcctgc accatggcgg
     4561 tgtagacgat catcaccccc agcagcgcgc cgcacaggcc cagcaccagc agcatgggcc
     4621 gcggaccacg cacgccatcg aacatgaagc gcagcgccat cggcccgatg atcagcgcca
     4681 ccgccacgcc ggtgcgcggc aggtcgcgca cgccccacat ggcgaccagg ctggcggtca
     4741 tggtcatgta caggccgagc agcgcgtgca ggccatgttc gcccggcacg tacagcagaa
     4801 agccggcgct gccgaccgcc aaagcggatt gccagacgta gcggcgataa atggcgcgcc
     4861 tgcgcgcttc gggcaacagc ttgaagtcgg gccgccgaac gcgccagaaa ccccaggtgc
     4921 cggtgcctgc gaccagcgtg aaccacagca tgggctgcca ccacggcagc accggcaaaa
     4981 acagcagcgc gcccagcagc tcgcaggccc ggatcagtat gtacagctgc accggctgcg
     5041 tcgcgccccg gtgttcgggc ggcggcagca gcaggctcac gcggtgtcgg ggttgtactg
     5101 cgcggcctgg gtgcggttgg acacgctgag cagccggaag atgtccgaca tgtagttctt
     5161 caccgtgcct tccgacagat cgagttgctg agcgatgtgc ttgttggaca ggccctggcg
     5221 cagcagcgcg cgaatttcct gcatgcgcgg tgtcagaccg gtatccggca aggcctcggc
     5281 cgccgccgca tcgggcagcc aggggtcgag ccggcggccc tgcagtgcgg cctcgatggc
     5341 ctcgcgcatg cgcgccacgc cggcgctctt gggcacgaag gcgcacacgc tgggcacgtc
     5401 gagcgcgcgc cgcaccacgt cgggcgaggt gagggcggag accacgatga gcggcaggcg
     5461 tggctgctcg cgcgccagcg aggcgagccg cagcccgcgc tccatgccgg gcatgttgag
     5521 gtcgaccagc gccagctgcg gcgtcggcac ccggcgcgcc agctgccaca gcgccggcgc
     5581 gttgccggcc tcgagcacct gcgcgcccgg cagcatggcg cacaccatca gcttgagtcc
     5641 gtcgcgcacc aggtcatggt catcggccag gatcaccgtc ggtgcggtgc cggcgccgga
     5701 aagaggagcg gccacggatg tcatcgccac agcgtatcga cccaggacgg atggcgcaat
     5761 cgtggcgggg gtgaaattgg tcatgcccgt cccctgacca aggtacgtgg caggtgcgcg
     5821 gcagcgcttg tggcgtgggc gcggattttg aagtgttttc aggtggcagc gcttgtcccg
     5881 cttgcgcgag cagctatcaa aatcatatca atcctcgtcc tgcaccttgt gctgccccat
     5941 cagctgctgc tgcacgccgg gtggcaccac ctcgtagtgc gacagcgcaa gcgagtagcg
     6001 gccctggccg gcggtcatgg cgttcaggcg ggtctggtag tcggccagct cggccatcgg
     6061 cgcctggccg ttgacggtga gctggcccaa ctggcccgaa tcggtgccgc tgaccatgcc
     6121 gcgccgcgcc gacagatctc ccgtgatggc gcccatcgcg ctttcgggtg cggcaatctg
     6181 cacctgggcg attggctcca gcaccactgg ctttgcttcc cggatggcgg cctgaaaggc
     6241 gcgcttgccg gcggtgacga aagcgatctc cttgctgtcg accgcgtggt gcttgccgtc
     6301 gtgcacaatg acgcgcacgt cgtgcaccgg gtagccggcg atcacgcctt cggccagtgc
     6361 ctcgcgcacg cctttttcaa ccgccggcat gaactggccg gggatggtgc cgcccttgac
     6421 ctgatcgaca aactcaaagc ccgcgccgcg cggcagtggc tcgatgcgca ggtgcacttc
     6481 gccaaactgg ccggcgccgc ccgactgctt cttgtggcgg tattgcgcgg cggcgttttg
     6541 cgtgatggtc tcgcggtagg cgatgcgcgg cgggcgcgtg tcgacttcga acttgtgcac
     6601 atcgcgcagg cgctccagca gcatgcgcag gtgcaactcg ccgaggccgt agaccacggt
     6661 ctcgttggtc tgcgccaggt gctccagctt gaggcacggg tcttcggcca ccagcttgcc
     6721 gacgatgtcc cacatgcgct gttcgtcgcc gcgccgcttc gggctgatgg cgacgccgtg
     6781 caccggcacg gggaagggca ggggcttgag gtggatgtgg tcgtcttccg ccgcgtcgtg
     6841 cagcacggcg ttgaaatgca gctcttcgat cttggcgacg gcgcaaatgt cgcccgggcc
     6901 ggcgctgggc acctcgacgt ggttcttgcc ttgttgcaga tacacgtgcc cgaccttgaa
     6961 cggcttgcgc ccgttgccca cgtacagctg cgtgccgggc gtgatgctgc cctggtgtac
     7021 gcgcacaaag cccagcttgc cgacgtaggg gtcgatactg accttgaaca cgtgcgccag
     7081 cacgtgcgcc gccgggtcgg gcttggcgtg catcagcgtt gcgtcggcgc cttcaccgtt
     7141 gagaaagtca ggcgggttgc cctcggtcgg gttgggcagc aggcgggcga tcacatccag
     7201 caattccggc atgccggcgc ccgtgcgggc cgagacaaaa cagaccggga tcaggtgccc
     7261 ctcgcgcagc gcctgctcca gcggcgcgtg cagctcgttg gcgtcgatgt cgccgtcgtt
     7321 caggtagcgg tcgacaaagt cgccatcgac ctcgacgacc tgctcgacca gcgcacggtg
     7381 cgcatcggcc acggcgccga agtcgcagtg gccttcgcgg ttgtaaaagc aatcgaccac
     7441 ctgggtggcg ccggcatccg gcaggttcaa gggcaggcat tccttgccga aggcggcctg
     7501 gatgtcggcc agcacagcgg acagatcgac gtcttgcgcg tcgatccggt tgacgatgac
     7561 gaggcggtcc agcccgcgct cggcggcgta ggccatcatg cgctgggcca tcggctcgat
     7621 gccggcggcg gcgttgatga cgatggcggc ggtttccacc gcttccagcg ccggcaggct
     7681 ctggccgatg aaatcgggcg cgccgggcgt gtcgatcagg tggatgcggc agccgccgcg
     7741 atcgaggtgc atcaccgacg attgcagcga atgcccggcc ttgatctcca gcgcgtcgtg
     7801 gtcgctgacg gtgctgccgc gctccacgct gccggccgcg cccatggcgc cggtgtgctg
     7861 caacaaggcc tcggccagcg tggtcttgcc ggcggcggcg gggccaacca gggccagggt
     7921 tcggatgttc ccgacggccg gcgtggccga tgtggtgctg ggcatggcgt gactcctgat
     7981 aatgactggc ggtgctgggc cgggcgctgc gacgctggcg acacttgggc ttgatagcgg
     8041 gtcagcggcg cctggcattc agcgcgtggc gcccatcagg tgcctggcca gcgcgtgata
     8101 ggccggcagc gatgtcgggt cgttgttggc gttggccgcc agcgggtgcg aggcgtagcg
     8161 cgcgatcacc atttccgccg tcggatcgac gtaaatcgcc tggccgtgaa tgccgcgcgc
     8221 catgtacgca ccgtgttcgt tgtgcgtgac ccaccacatg ttgcggtaac tccagccggg
     8281 caggctgacg tagccagccg gtgtgaaggc agcgcggtcg ccaccgcggc ggatgtcgtc
     8341 caccaccgcc tgccggatca cctgccggcc gtcgaacagg ccgccccagc agccgccgca
     8401 gcgcatcagc tcgccaaagc gggccagatc gcgcaagccg gtgttgaggc caccgccggc
     8461 aaactcggtg cccaccgagt cgacggtgaa gtaggcgtcc tgctccatgc ccaggcgcga
     8521 ccagatgcgc tcggacagca cctgccccgc cgacttgccg gtgatgcggc ggatgaccca
     8581 gcccagcacg tcggagttga ccgtcttgta agcgaacgcg ccgccgtgct cgccttcctt
     8641 ttgcaccatc tgcaggaact cgtagaagct ctgcggcccc tggtagccgg gcggacgcgg
     8701 cagcacgttg ccggcgcgcg cgtgcatcca gatctcggcc ttgggatcgg cgtagttctc
     8761 gctgtacttg aggccggtgc gcatgtccat cacctggcgc acggtggcgt cggcgaaggc
     8821 gctgcccttc agctcgggca cgtactgcgt caccagggcg ttctggtcaa tctgcccgtc
     8881 ggccaccagc atggcggcga tggtgccgaa gaaggacttg gtgagcgatt gcgacagatg
     8941 ctgcccgtcg ggcttgaaca cgccggcgta gcgctcgtac acgatgcggc ccttgtgcag
     9001 caccaccacg ccatcggtgt agttggcgtc gaaggccttg ccccaggtca tgggctcggt
     9061 catgcccagc ggcacgaagg tgacggcggc gaggtcggcg cgctcggcgc gcggcagcgc
     9121 agacggcgag tccagcccgc gcgagacgtt ggtggtggca cccagctggc gcgcgttgga
     9181 gaaagtccag cgcagttgcg gaaagcggta gaagctgccg tcttccttgc gcacgatctt
     9241 gtcggccggc gggggcgagc cgaccatcca gcccagccgt gccgggtccg atgcctgcgc
     9301 atcgggtggg ggcgccgttt gggccagggc gccggcgctc acgcaggcgc agaccagccc
     9361 ggcccaccgg gcgcaggcgt attgccgtcg aatcatgctt gtctcctcgt tcttgtaggg
     9421 cgaatggctc gcgttgccat cataagactg cgcagcgtgc gctatgctga cccgcatgac
     9481 cgagtccatc gctaccccgc aggacgtgct ggccttctgg cttggcgcgt ggccgattga
     9541 tcaagccgcc atgcagcgcg tgcaggcaca gtggttcagc aagaacgacg ctttcgatgc
     9601 cgagctgcgc cagcgctttg gtgccaccct cgccgccgcg cgcgccggtc ggctggacga
     9661 ctgggcgcgc gacgccgacg gctggctggc gctgctcatc gtgctcgacc agttcagccg
     9721 caacgcgttt cgcggccagc cggagggctt tgcgggcgat gcgcaggcgc tggcgatcgc
     9781 ccaagccggt atcgcacgtg gcgatgacca gcgcgtgccg ccgatggcgc gcatcttctg
     9841 ctacctgccg ctggagcacg cggaagacgc cgccctgcag gctcgaagtg tggcgctgtt
     9901 cactgcgttg cgcgatgcgc cgggcgctga gccgaaggca tttttcgacg gcacgctgga
     9961 ctacgcgcac aagcaccagg acgtgatcgc gcgctttgga cgctttccac atcgcaacgc
    10021 gattctggcg cgtgccagca cggccgaaga gcaggcctac ctggcgcagc cgggcgctgg
    10081 tttttgaatc aaatcaggct gtagcgcttg cccatcaagc gcgagcagct atcgttggtg
    10141 tagcaatcag cgcaccggct cgatgcgcac caaccattcc gcttcgccca tgtcggcgcc
    10201 cgcgttgcag tggcggctga ccacgcggta gacgcggttg cggtggttgg gaaagctcac
    10261 cgtgtcgccc acgctcaaag cgcacagggg gatgtccgag tcgggcgttg gcagcagccc
    10321 ggtggtggtc cgaaaaccgg gcgtgtcctg atcgatgtag aacgagatga cgctcatgcc
    10381 agcctcctgt ggggtggctg cattgtgcag cggcaaattg aatttggcga tgcgtctcag
    10441 tgatccacgg cgctggtgca cgccacgcgg gaacgccgtg gagcgggctt ggcccggcca
    10501 ccggcgttgt ccccctgggg gatggcgccg aaggcgaatc aggggcgctt tatctcagcc
    10561 cagcatttcg aagtcgatga agccgcgctc gacgttggtg gaccgcagtt tgacgcgcac
    10621 cttgtcgccc acgttgatct tgcgtccgcg cgccaccagc agcttgcctt cggcgggtgg
    10681 gttgaagatg cgcacccagt tgttgccgtt cgaactgccg gtcaccacgc cgtcaaactc
    10741 gtcgccgatg tgcgattcaa gcagcatggc ggcttctgat ttgcgcaact ggcgctcgac
    10801 cttgttggcg gcgtcttcct gcttggtgca gcggttggcg aggaattcca gctcggtgtg
    10861 cgagtagggc gctgccgctt ctgacagcgc ggccttgatc aggcggctgg tgatgaggtc
    10921 ggggtagcgg cggttgggcg ccgtgctgtg cgaatagtcg cgcacggcca ggccaaagtg
    10981 gccaatcgtc gggccgccgg gccgctcgac cacgtattcg ccgcggccca tcagcttgac
    11041 gatgatgagc gacagatcgg gaaagcgcag cggatcgcgc ttgcgctcgg cggccagaaa
    11101 ctgctccagc gccgctgaat tgggctcggg cggcaattgc cagccccgct cggccgccac
    11161 ttcggcaatg cgcgcccagc gctcgggcga cttcaccacg cggcggatcg acgcgcgttt
    11221 cttgcccgcc agaaagcgcg cggtgacgcc gttggtggcc accatgaatt cctcgatcaa
    11281 ctggcgcgcg cggttctgca cctgctgctt gatggccacc acct
//
LOCUS       DAOMVO010000044        11037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1418_length_11037_cov_12.279366, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000044 DAOMVO010000000
VERSION     DAOMVO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1418_length_11037_cov_12.279366"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /gene="purL"
                     /locus_tag="PKV30_03805"
                     /gene_synonym="purI"
     CDS             complement(<1..2540)
                     /gene="purL"
                     /locus_tag="PKV30_03805"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRL29379.1"
                     /translation="MHIHFFDGGNALSDFRVRQLLPRLQAVNAGVESIRARHVHLVGF
                     DQAPNADELDRLGALLTYGEPFAAGSAAPDAAVVVAPRLGTVSPWASKATDIALNCGL
                     NPHRVERLVEYKIGLQRKLIGRHQLSKSDLDAVAALLHDRMTESVLASRDEARALFTE
                     LHPEPLAHVDVLAGGRAALEQANTDWGLALAADEIDYLMDAFTKLGRNPTDVELMMFA
                     QANSEHCRHKIFNAQFTIDGVAQDKSLFGMIRHTEQQNPQHTVVAYSDNAAVMEGGTV
                     EVFQAQSASGAGGTNARSYQKDTATRHVLMKVETHNHPTAISPFPGASTGAGGEIRDE
                     GATGRGARPKAGLTGFTVSKLWDSAVGKPEHMASPLQIMTEGPLGGAAFNNEFGRPNL
                     VGYFREYELQAGGVQRGYHKPIMIAGGIGSIDAGLTHKIEFPAGSLLIQLGGPGMRIG
                     MGGGAASSLASGANAAHLDFDSVQRGNPEIERRAQEVITQCQALGADNPILAIHDVGA
                     GGLSNAFPELTNDAGRGARFDLRAVPLEESGLAPKEIWSNESQERYVMAIAPESLALF
                     EALCARERCPFAVVGVATQERQLVVGDLDLPLPPLGEGGDGGSPVTSTLGLPPPQPSP
                     SGGGSQAVDMPMDVLLGKPPKMHRDVTTLTREFTPLNLDGVTLQDAVIKVLSHPTVAS
                     KRFLITIGDRTVGGLTHRDQMVGPWQIPVADCAVTLADFKGFAGEAMSMGERTPLAAL
                     DAPASGRMAVAEAITNLLAAPIELPRVKLSANWMAACGEPGEDAALYATVKAVGMELC
                     PALGVSIPVGKDSLSMRTQWQAEGAAQKVVSPVSLIVSAFASVQDVRGT"
     gene            complement(2555..2992)
                     /locus_tag="PKV30_03810"
     CDS             complement(2555..2992)
                     /locus_tag="PKV30_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29380.1"
                     /translation="MSHLLDANALIALCWPPHEHQERMRDWFGRHAAEGWATCALTQA
                     AFVRLLCQPVIAGRAMAMADVAELLLRNTAHPKHRYLESAPDLATVLAVCTGGLMGHR
                     QVTDAWLLTAAVQNGMKLLSCDHGVPMLLATAAERQRHIEHPA"
     gene            complement(2989..3216)
                     /locus_tag="PKV30_03815"
     CDS             complement(2989..3216)
                     /locus_tag="PKV30_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29381.1"
                     /translation="MRTTITLESDAFAAAQVYAQARALKLGQAVSELILQSTAQKLAL
                     VEKNGIWVYDLPADTPRVTAAQVKALLDDTP"
     gene            complement(3261..3512)
                     /locus_tag="PKV30_03820"
     CDS             complement(3261..3512)
                     /locus_tag="PKV30_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29382.1"
                     /translation="MDASAAQHEGGERWQRLMAGFAQPGFSAFDDTATDLLRRAGVKG
                     SRVVQVGCNIGGEVLSCPIAAALLNGRAVVGRRSHPALS"
     gene            3670..4491
                     /gene="map"
                     /locus_tag="PKV30_03825"
     CDS             3670..4491
                     /gene="map"
                     /locus_tag="PKV30_03825"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRL29383.1"
                     /translation="MSITIKDEAGIAGMREACRIASQVLDDITPHIRPGITTLDIDRL
                     SAESMARHGTRSATIGYQPPGYPPYPGHVCTSVNHVVCHGIPADKPLKKGDIVNVDVT
                     VITADGWYGDNSRMFEIGEVSIAARRLCALTFDAMWLGIDQVRPGARLGDVGHAIQKF
                     VEGHGLSVVREFCGHGIGQRFHEDPQVLHYGRPGTGEELVPGMTFTIEPMVNIGRRDI
                     KEMNDGWTIVTKDRSLSAQWEHTVLVTPTGYEVLTLSEGSPATPDFVQGMARAAA"
     gene            4515..7205
                     /locus_tag="PKV30_03830"
     CDS             4515..7205
                     /locus_tag="PKV30_03830"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRL29384.1"
                     /translation="MPTDAALEPRVPALRALYREQKTAMLDSLRAGGAGTRGIRGALT
                     RLAGLTDASLRSLWQANGFGGDLALLAVGGYGRGELFPCSDVDVLLLLPDAADPARDT
                     ALRQRIESFIGQCWDIGLEIGSSVRTVPECLSVAQGDVTVQTALLEARLIVGNRRLFK
                     GFQARFSAQLDALDFFSAKRLEMRQRHAKYEDTPYALEPNIKESPGGLRDLQIILWTA
                     RAAGIGGRWEDLHRAGLVTTHELRQLRHNEALLSAIRSRLHLIAARREDRLVFDLQNA
                     VAESFGFQHRLADDGRVMLRASEVLMRRYYWAAKAVTQLNQILLQNIEEHLRAARGEA
                     APEQRRINERFFDKGGMIEVASDDLYQREPQAILETFLLYAKTPGLKGLSARTLRALY
                     NARTVMDHGFRTDPANRKTFLAILQQPQGITHAFRLMNQTSVLGRYLWVFRRIVGQMQ
                     HDLFHVYTVDQHILMVLRNVRRFFIPEHAHEYPFCSQLAAGWDKPWVLYVAALFHDIA
                     KGRGGDHSELGAGEVRRFCREHGVQQADAQLIEFLVSHHLLMSRIAQKEDLSDPEVIE
                     AFARQVGNERYLTALYLLTVADVRGTSPKVWNAWKGKLLEDLYKLTVRTLGGRAPDPG
                     AVIESRKRQALIQLALASEPQDSHKRLWDTLEVSYFMRHDAGEIAWHTRQLARHAVRP
                     VATNTEAVGAGGASAGTVKTFETIVRARLSPVGEGLQVLVYAADQPDLFARICGYFDR
                     AGFSILDAKIHTTRNGRALDTFQIINPLVPDHNREFIGMVEAELPAAVESQAPLAPPR
                     LGRVSRRVKSFPIAPRVDLRPDDKAQRWLLTVTASDRVGLLYSIARVLAAHGINLELA
                     KVSTLGERVEDMFLLQGAQLQHNKTQLAIEQELLDVLEPQ"
     gene            complement(7213..11019)
                     /locus_tag="PKV30_03835"
     CDS             complement(7213..11019)
                     /locus_tag="PKV30_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HRL29385.1"
                     /translation="MKLMTDGILLAETQTDPLLRNYDTIIIDEAHERSLNIDFLLGYL
                     KQILPKRPDLKIVVTSATIDADRFAKHFADAKGQPAPVLMVSGRTYPVEQRYRPFQES
                     RDHDLNDAMADAVDEVWVGRPAGDILIFLPGEREIREAADHLRKHLAHSPLTRNAEVL
                     PLFARLSQAEQDRVFDTHGAPRIVIATNVAETSLTVPGIKYVIDAGTARVKRYSYRSK
                     VEQLLIEPVSQAAANQRAGRCGRVSDGVCIRLYDEKDFASRPRFTDPEILRSSLAAVI
                     LRMKSLHLGGEGGRVEDFPFLDMPSGRAIADGIQLLQELGAMDDAQELTALGAELSRL
                     PLDPRVGRMILEARERGALREVLIIAAALSVQDVRDRPLEQQAQADQAHAKFDDEKSE
                     FSGYLRLWKWLGDARAGQVVKTRGEMAAAEGRGAAKKPANLAALPAAQRAAMAMEAAR
                     RALQQPIEHRGTEAQRKEEKASVNLRASVPLCSVQPADAAPAHKLSNRQWESLLRQNF
                     ISIRRVREWRDIHTQLHTVVAEHKWKENTQPAGYEALHKSLLAGLLGNVGHRLENDDA
                     PSGEYLGARGIKFHRHPGAHLSKKPGRWIVAAELVETTRLFGRGIANIEPEWLEEVGG
                     HLLRKQLLEPHWEKKAQDVIALERATMYGLVVYSGRRRSFGHVDPRAAREIFIREALV
                     NGEWPEDWARRLPFLQANAGTIAKVEELEHKSRRQDVLVDDELIYAFYDAQVPPGISN
                     GRDFEAWWRDASRTQPNLLLLTRDELMRHEAAGITTSAFPKMIRLGGVDCSATYLHEP
                     GDAKDGLTVTVPLFVLNQVSEARCEWLVPGMLKEKIQALLKSLPQRPRSRFVPLPESA
                     ARLTEVLGTPEAFGHGSLTDALLKQVRDVTSLDVKRTDFKLDMLPAHLFMNFRVVDEH
                     GRQRGQGRNLAALKAELGGEARGAFQALAGLKAAKAEIVTPAAADSDQKAAPALEGQA
                     RAAIKTVESKPASGDTRHTSWDFGELPELMEIRRKGQTLVGFPALIDAGDAVTIEVFD
                     EPEVAAARHRAGLRRLFALQIKDALKYLEKNVPDLQKMAVAYMPLGTAEELRQQIIDV
                     ALDRAFLTDPLPATETDFKQRVDEGRARLTLIANEIARLAGTILTEYAAAARKIKDTK
                     SAPEATKDAAEQLQRLVPKNFLAATPWAALQHFPRYLKAIVLRLDKLRADPARDAQRM
                     QEARAQDQRFWRLVAERKGQQDARLQELRWLLEELRVSFFAQELRTPQPVSVKRLDKV
                     WGQLAA"
BASE COUNT         1755 a         3747 c         3624 g         1911 t
ORIGIN      
        1 gtgccgcgca cgtcttgaac cgaggcaaag gcgctgacga tcaggctgac gggcgagacg
       61 actttttggg ccgccccctc cgcctgccac tgcgtgcgca tcgacaggct gtctttaccg
      121 acgggaatcg acacgcccag ggccgggcac agctccatgc cgacggcctt gacggtggcg
      181 tacagcgccg cgtcttcacc cggctcgccg caggcggcca tccagttggc ggacagcttg
      241 acgcgcggca attcaatcgg cgccgcgagc agattggtga tcgcctcggc cacggccatg
      301 cggcccgatg cgggcgcatc cagcgcggcc agcggcgtgc gttcgcccat gctcatcgct
      361 tcgccggcga agcccttgaa atcggccagc gtcacggcgc agtcggccac cggaatttgc
      421 cacggcccga ccatctggtc gcggtgcgtg aggccgccca cggtgcggtc gccaatggtg
      481 atgagaaagc gcttgctggc cacggtgggg tggctcaata ccttgatgac ggcgtcttgc
      541 agggtcaccc catccaggtt gagcggcgtg aattcgcgcg tcagggtggt tacgtcgcgg
      601 tgcatcttgg gcggcttgcc gagcagcacg tccatgggca tgtcgacggc ttggctccct
      661 cccccgctgg gggagggttg gggtgggggc aagcccagtg tgctggtgac ggggctgccc
      721 ccatccccac cttcccccag agggggaagg ggcaaatcca ggtcgccgac gacgagttgc
      781 cgctcctgcg tcgctacccc caccaccgca aacgggcatc gctcgcgcgc gcacaacgcc
      841 tcaaacaacg ccagcgattc cggcgcaatc gccatcacat agcgttcctg gctttcgttg
      901 ctccagattt ccttgggcgc caggccgctt tcttccagcg gcacggcgcg cagatcaaag
      961 cgcgcaccgc gacctgcatc gttcgtcaat tcaggaaaag cattcgacaa gccacccgcc
     1021 cccacgtcgt gaatcgccag gatcgggtta tccgcgccca gcgcctggca ctgcgtgatg
     1081 acctcttgcg cgcggcgttc gatctccggg ttgccgcgct gtaccgaatc aaaatccagg
     1141 tgcgccgcgt tggccccgct ggccagcgac gacgccgcgc cgccgcccat gccgatgcgc
     1201 atgccgggac cgcccaactg aatcagcagg ctaccggccg gaaactcgat cttgtgcgtc
     1261 aagcccgcgt cgatgctgcc aatgccaccc gcgatcatga tcggcttgtg atagccgcgc
     1321 tgcacgccgc cggcctgcag ctcgtattcg cggaaataac cgaccaagtt gggccgcccg
     1381 aattcgttgt tgaaagccgc gccgcccagc ggcccctcgg tcatgatttg cagcgggctg
     1441 gccatgtgct cgggcttgcc gaccgccgaa tcccacagct tgctgacggt gaaacccgtc
     1501 agccccgcct tgggccgcgc gccgcgcccg gtcgcgcctt catcgcgaat ctcgccgccc
     1561 gcgccggtgc tggcgccggg gaaggggctg atcgccgtcg ggtggttgtg cgtttccacc
     1621 ttcatcagca cgtggcgcgt ggcagtatct ttttgatagc ttctcgcgtt cgtcccgcct
     1681 gcgccagagg ccgattgggc ttgaaaaacc tcgaccgtgc cgccttccat caccgccgcg
     1741 ttgtcgctgt aggcgaccac ggtgtgctgc gggttttgct gctcggtgtg gcggatcatg
     1801 ccgaacaggc tcttgtcctg cgccacgccg tcgatggtga actgcgcgtt gaaaatcttg
     1861 tgccggcaat gctcactgtt ggcctgcgcg aacatcatca gctccacatc ggtcgggttg
     1921 cggcccaact tcgtgaaggc gtccatcaaa tagtcgatct cgtcagccgc cagcgccagg
     1981 ccccagtcgg tgttggcctg ctccagcgcg gcgcggccac cggccagcac atcgacgtgc
     2041 gccagcggct caggatgcag ctcggtgaac agcgcgcgcg cctcgtcgcg gctggccagc
     2101 acgctctcgg tcatgcggtc gtgcagcagc gcggcgacgg catccagatc actttttgat
     2161 agctggtggc gcccgatcag cttgcgctgc aggccgattt tatattcaac caggcgctcc
     2221 acgcggtgcg gattgaggcc gcagttgagc gcgatgtcgg tcgccttcga cgcccagggc
     2281 gacacggtgc ccaggcgcgg cgcgacgacg accgccgcgt caggcgccgc gctgccagcg
     2341 gcgaaaggct cgccgtaggt cagcaacgcg cccagtcgat ccagctcgtc cgcgttcggc
     2401 gcctgatcga accccaccag atgcacatgg cgcgcgcgga tcgactccac cccggcgttc
     2461 accgcctgca agcgcggcag cagttgccgc acgcgaaagt cgctcagggc gttgccgccg
     2521 tcgaagaaat ggatgtgcat ttgaaaaact cgggttaagc gggatgttcg atgtggcgct
     2581 ggcgttcggc ggcagtggcc agcagcatcg gcacgccatg atcacagctg agcagcttca
     2641 tgccgttttg aacagccgca gtcagcagcc aggcatcggt gacctggcgg tggcccatca
     2701 ggccgccggt acacacggcc agcacggtgg ccagatcggg cgcggattcg aggtaacggt
     2761 gtttcgggtg cgcggtgttg cgcagcagca gttcggccac atccgccatg gccatggcgc
     2821 ggccggcgat cacgggctgg cagagcagcc gcacaaaggc ggcttgcgtt aaggcgcagg
     2881 tggcccaacc ttcggccgca tggcgaccaa accagtcgcg catgcgctct tggtgttcat
     2941 gcggcggcca gcacaacgcg atgagcgcgt tggcatccag caagtggctc aaggcgtgtc
     3001 gtccagcagc gccttgactt gcgcggccgt cacgcgcggc gtgtcggccg gcaggtcgta
     3061 cacccagatg ccgttttttt ccaccagcgc cagtttctgc gctgtggatt ggaggatcaa
     3121 ctcggacact gcctgcccga gcttcagtgc cctcgcctgc gcgtagactt gcgcggcggc
     3181 aaacgcatcg ctttcgaggg taatggtggt acgcatctga tgcactcgcg tcgatttgat
     3241 gccattttac cgtgagcgca tcaactcagg gctgggtgac ttctgcgccc aaccactgcc
     3301 cgaccattga gtaacgcagc ggcaattggg cacgagagca cctcgccgcc gatgttgcaa
     3361 ccgacctgca ccacgcggct gcctttgacg ccggcgcgac gcagcaagtc ggtggcggtg
     3421 tcgtcgaaag cggaaaaccc cggctgggca aagcccgcca tcaagcgctg ccagcgttcg
     3481 ccgccttcgt gctgagcggc cgaggcatcc acgcggcgcg gttggattgg gggtaggcgg
     3541 tcaggttttt cagggcgact ggcggcctgc gcacgatatg gacaaagggc caacgcggcg
     3601 gcgtgggcaa cccgcggttt tgccgccgcg gccctgcgcc cagaggccgc ccgaaggata
     3661 atctcgccca tgagcatcac gatcaaagac gaagccggca tcgcaggcat gcgcgaggcc
     3721 tgccgcatcg cgtcgcaagt gctggacgac atcacgccgc acatcaggcc gggcatcacc
     3781 acgctcgaca tcgaccgcct gagtgccgaa tcgatggcgc gccacggcac gcgctcagcc
     3841 accatcggct accagccgcc gggctacccg ccctaccccg gccacgtgtg cacttcggtc
     3901 aaccacgtgg tgtgccacgg cattccggcc gacaagccgc ttaaaaaagg cgacatcgtc
     3961 aacgtcgatg tcacggtgat caccgccgac ggctggtacg gcgacaacag ccgcatgttc
     4021 gagatcggcg aagtctcgat tgccgccagg cgcctgtgcg cactgacgtt tgacgccatg
     4081 tggctcggca tcgaccaggt caggcccggc gcgcgactgg gcgacgtggg ccacgcgatc
     4141 cagaagttcg tcgaagggca tggcctgtcg gtggtgcgcg agttctgtgg ccacggcatc
     4201 ggccagcgct ttcacgaaga cccgcaggtg ctgcactacg gccggccggg cacgggcgaa
     4261 gagctggtgc cgggcatgac cttcaccatc gagcccatgg tcaacatcgg ccggcgcgac
     4321 atcaaggaga tgaacgacgg ctggaccatc gtcaccaagg accgcagcct gtcggcgcag
     4381 tgggaacaca cggtcctggt cacgcccacc ggctacgagg tgctgacgct gtccgaaggc
     4441 agcccggcca cgcccgactt cgttcagggc atggcgcgcg ccgccgcctg accgggcatc
     4501 cgcgaccttc ccccatgccg acggacgccg ccctcgaacc gcgggtgccg gcgctgcgcg
     4561 ccctctaccg cgagcaaaaa accgccatgc tggacagcct gcgcgccggc ggcgcgggaa
     4621 cgcgcggcat ccgtggcgcg ctgacccggc tggcgggcct gaccgacgcc tcgctgcgca
     4681 gcctgtggca ggccaacggc ttcggcggcg acctggcgct gctggcggtg ggcggctacg
     4741 ggcgcggcga gctgtttccg tgttcggacg ttgacgtgct gctgctgctg cccgacgcgg
     4801 cggacccggc gcgcgacacc gcgcttcgcc agcgcatcga aagcttcatc gggcagtgct
     4861 gggacatcgg gctggagatc ggctccagcg tgcgcacggt gcccgaatgc ctgtcggtag
     4921 cgcagggcga cgtgacggtg cagacggcgc tgctcgaagc gcgcctgatc gtcggcaacc
     4981 ggcggttgtt caagggcttt caggcgcgct tttcggcgca gctcgacgcg ctggattttt
     5041 tcagcgccaa gcggctggag atgcgccagc gccacgccaa gtacgaggac acgccctacg
     5101 cgctcgagcc caacatcaag gaatcgcccg gcggcctgcg cgatttgcag atcatcctgt
     5161 ggacggcacg cgcggccggc atcggcgggc gctgggaaga cctgcaccgc gccggcctgg
     5221 tcaccacgca cgagctgcgc cagctgcgcc acaacgaggc gctgctgtcg gccattcgca
     5281 gccgcctgca cctgatcgcg gcgcggcgcg aggaccggct ggtgttcgat ttgcagaacg
     5341 cggtggccga atccttcggc ttccagcacc gcctggccga cgatggccgc gtgatgctgc
     5401 gcgccagcga ggtgctgatg cgccgctact actgggccgc caaggcggtg acgcagctca
     5461 accagatcct gctgcagaac atcgaggagc acctgcgtgc cgcgcgtggc gaggcggcgc
     5521 ccgagcagcg ccgcatcaac gaacgcttct tcgacaaggg cggcatgatc gaagtcgcca
     5581 gcgacgacct gtaccagcgc gagccgcagg caattctgga gacctttctg ctctacgcca
     5641 agacgcccgg cctgaagggc ctgtcggcac gcacgctgcg cgcgctgtac aacgcgcgca
     5701 cggtgatgga ccacggcttt cgcaccgacc cggccaaccg caagaccttt ctggccatcc
     5761 tgcagcagcc gcaaggcatc acgcacgcct ttcggctgat gaaccagacc tcggtgctgg
     5821 ggcgctacct gtgggtgttt cgccgcatcg tcgggcagat gcagcacgat ctgtttcacg
     5881 tctacacggt cgaccagcac atcctgatgg tgctgcgcaa cgtgcggcgc ttcttcatcc
     5941 ccgagcacgc gcacgaatac ccgttctgct cgcaactggc ggccggttgg gacaagccct
     6001 gggtgctgta cgtggcggcg ctgttccacg acattgccaa ggggcgcggc ggcgatcact
     6061 ccgagctggg cgccggcgag gtgcggcggt tttgccgcga acacggtgtg cagcaggccg
     6121 atgcgcagct gatcgaattc ctcgtcagcc accacctgct gatgagccgc atcgcgcaga
     6181 aggaagactt gagcgacccc gaggtgatcg aggccttcgc gcgccaggtc ggcaacgagc
     6241 gctacctgac ggcgctgtac ttgctgacgg tggccgacgt gcgcggcacc agccccaagg
     6301 tgtggaacgc ctggaagggc aagctgctgg aagacctgta caagctgacg gtgcgcacgc
     6361 tgggcgggcg ggcgcccgac cccggcgcgg tgatcgaatc gcgcaagcgg caggcgctga
     6421 tccagctggc gctggcgtcc gagccgcagg attcgcacaa gcgcctgtgg gacacgctgg
     6481 aggtgagcta cttcatgcgc cacgacgccg gcgaaatcgc ctggcacacg cgccagctgg
     6541 cacgccatgc ggtacgcccc gtcgctacaa acacagaagc tgttggcgca ggcggggcaa
     6601 gcgctgggac cgtaaaaacc tttgaaacga tcgtgcgggc ccgactctcg ccggtcggcg
     6661 aagggctgca ggtgctggtc tacgccgccg accagccgga tctgtttgcc cgcatctgcg
     6721 gctacttcga ccgcgcgggc ttttccatcc ttgacgccaa gatccacacc acgcgcaacg
     6781 gccgcgcgct ggacaccttc cagatcatca acccgctggt gcccgaccac aaccgcgagt
     6841 tcatcggcat ggtcgaggcc gaactgcccg ccgccgtcga aagccaggcg ccgctggcgc
     6901 cgccgcgcct gggccgcgtg tcgcgccgcg tcaagagctt tccgatcgcc ccgcgcgtcg
     6961 acctgcggcc cgacgacaag gcccagcgct ggctgctcac cgtcaccgcc agcgaccgcg
     7021 tgggcctgct ctacagcatc gcccgcgtgc tggccgcgca cggcatcaac ctggagctgg
     7081 ccaaggtctc caccttgggc gagcgggtgg aagacatgtt cttgctgcaa ggcgcgcaac
     7141 tgcagcacaa caagacgcaa ctggccatcg agcaggaatt gctcgacgtg ctggagccgc
     7201 agtaaacgcc gctcaggccg ccagctgccc ccacaccttg tcgagccgct tgacgctgac
     7261 cggctgcggc gtgcgcagct cctgcgcgaa gaaggacacg cgcaactcct ccagcagcca
     7321 gcgcagctct tgcagtcgcg cgtcttgctg gcccttgcgc tcggccacca ggcgccagaa
     7381 gcgctggtct tgggcgcgcg cctcctgcat gcgctgggcg tcgcgcgcgg ggtcggcgcg
     7441 cagtttgtcc aggcgcagga cgatggcttt caggtagcgc gggaagtgct gcaacgcggc
     7501 ccacggcgtg gcggcgagga agttcttggg caccaggcgt tgcagctgct cggcggcgtc
     7561 ttttgtggcc tcgggcgcgc tcttggtgtc cttgatcttg cgtgcggcgg cggcgtattc
     7621 ggtgaggatg gtaccggcca agcgcgcgat ctcgttggcg atcagggtca ggcgcgccct
     7681 gccttcgtcc acgcgctgct tgaagtcggt ctcggttgcg ggcaacgggt cggtgaggaa
     7741 ggcgcggtcg agggcgacat cgatgatttg ttggcgcagc tcttctgccg tgccgagcgg
     7801 catgtaggcg acggccattt tctgcagatc gggcacgttc ttttcaaggt acttcagcgc
     7861 atccttgatc tgcagcgcaa acaggcggcg caggccggcg cggtgcctgg cggcggcgac
     7921 ctcgggttca tcgaacactt caatcgtcac cgcatcgccc gcgtcgatca gcgccggaaa
     7981 gccgaccagc gtctggccct tgcggcggat ttccatcagc tcgggcagtt cgccaaagtc
     8041 ccaggacgtg tggcgcgtgt cgcccgaggc aggtttgctt tctacagttt tgatagctgc
     8101 tcgcgcttgt ccctcaagcg ctggcgccgc tttttgatct gaatctgccg cagccggcgt
     8161 cacgatctcc gccttggccg ccttcagccc cgccagcgcc tgaaacgcgc cgcgcgcctc
     8221 gccacccagc tcggctttca gcgccgccag gttgcgcccc tgcccgcgct ggcggccgtg
     8281 ctcatcgacc acgcggaagt tcatgaacag gtgcgcgggc agcatgtcga gcttgaagtc
     8341 ggtgcgcttg acgtccaggc tggtcacgtc gcgcacctgt ttcagcagcg catcggtcag
     8401 gctgccgtgg ccgaacgctt cgggcgtgcc cagcacttcg gtcaggcgcg cggcgctttc
     8461 gggcagcggc acgaagcggc tgcgcgggcg ctgcggcagg cttttgagca gtgcctgaat
     8521 cttctccttc agcatgcccg gcaccagcca ttcgcagcgc gcttcgctca cctgattgag
     8581 cacgaacaga ggcaccgtca cggtcaggcc gtccttggcg tcgcccggtt cgtgcaggta
     8641 ggtcgcgctg caatcgacac cgcccaggcg aatcatcttg gggaaggcgc tggtggtgat
     8701 gcccgcggct tcgtggcgca tcagctcatc gcgcgtgagc agcagcagat tgggctgcgt
     8761 gcggctggcg tcgcgccacc acgcctcgaa gtcgcgcccg ttgctgatgc ctggcggcac
     8821 ctgcgcgtcg taaaaggcgt agatcagctc gtcatcgacc agcacatctt gccggcggct
     8881 tttgtgctcc agctcttcga ccttggcgat ggtcccggcg ttggcttgca gaaacggcag
     8941 gcggcgcgcc cagtcttcgg gccattcgcc attgaccaat gcttcgcgga tgaagatctc
     9001 gcgcgcggcg cggggatcga cgtggccaaa gctcctgcgg cgcccgctgt agaccaccag
     9061 gccgtacatc gtggcgcgct ccagcgcgat cacgtcctgc gcttttttct cccagtgcgg
     9121 ctccagcaac tgctttcgca agagatggcc gccgacttct tccagccact cgggctcgat
     9181 gttcgcaatg ccgcggccga acaggcgcgt cgtctccacc agttcggccg ccacgatcca
     9241 gcggccgggc tttttgctga ggtgcgcacc ggggtgacga tggaacttga tgccgcgcgc
     9301 gcccaggtat tcgccgcttg gtgcatcgtc gttttcaagc ctgtggccaa cgttgcccag
     9361 caggcccgcc agcagcgact tgtgcagcgc ttcgtaccca gcgggctggg tgttttcctt
     9421 ccacttgtgc tcggccacca cggtgtgcag ctgcgtgtgg atgtcgcgcc actcgcgcac
     9481 gcggcggatg ctgatgaagt tctggcgcag caggctttcc cactgacgat tgctgagttt
     9541 gtgggcgggc gcggcatccg caggttgaac agaacacaga ggcacagagg cacggaggtt
     9601 cacagaggct ttctcttctt tcctctgagc ctctgtgcct ctgtgttcta ttggctgttg
     9661 aagcgcccgc cgcgccgctt ccatcgccat cgccgcccgc tgcgccgccg gcagcgcggc
     9721 cagattggcc ggcttcttcg ctgccccccg gccttcggct gccgccatct ccccccgcgt
     9781 cttcaccacc tgtccagcgc gcgcatcgcc cagccatttc cacaaccgca gatagccgct
     9841 gaactcgctc ttctcgtcgt caaacttggc atgcgcctga tcggcctgcg cctgttgctc
     9901 cagtggccgg tcgcgcacat cctgcacgct gagggccgcc gcgatgatca gcacctcgcg
     9961 cagcgcgccg cgctcgcgcg cctccagaat catgcggccc acgcgcgggt cgagcggcag
    10021 gcgcgacagc tcggcgccca gggcggtgag ctcctgcgcg tcgtccattg cgcccagctc
    10081 ttgcagcagt tgaatgccat cggcaatcgc gcggcccgag ggcatgtcga gaaacggaaa
    10141 gtcctccacc cgcccgccct ctccccctaa gtgcaggctc ttcatgcgca ggatgacggc
    10201 ggcgagcgac gagcgcagga tttcggggtc ggtaaagcgc gggcggctgg cgaagtcttt
    10261 ttcgtcgtac aggcggatgc acacgccatc gctcacccgc ccgcagcggc cagcgcgctg
    10321 gttggctgcg gcctggctga cgggctcgat cagcagctgc tccaccttgc tgcgatagct
    10381 gtagcgcttc acgcgcgccg tgcctgcgtc aatcacgtac ttgatgcctg gcacggtcaa
    10441 cgaagtctca gccacgttgg ttgcaatcac gatgcgcggc gcgccgtgcg tgtcgaacac
    10501 acggtcttgc tcggcttggg acaggcgcgc gaagagcggc agcacctcgg cgttgcgcgt
    10561 gagcgggctg tgcgcgaggt gcttgcgcag atggtcggct gcttcgcgaa tttcgcgctc
    10621 gccgggcaga aaaataagga tgtcgcccgc cggtcggccg acccaaacct cgtccacggc
    10681 atccgccatc gcgtcgttca ggtcgtggtc gcggctttcc tggaagggac gatagcgctg
    10741 ctcgaccgga taggtgcggc ccgacaccat cagcacgggc gcaggttggc ctttggcgtc
    10801 tgcaaagtgc ttcgcaaacc gatcagcgtc gatggtggcc gaggtcacga cgattttcag
    10861 atcgggccgc ttcggcagga tctgcttcaa ataccctagc agaaagtcga tgttcagact
    10921 gcgctcgtgc gcctcgtcga tgatgatcgt gtcgtaattt ctcagcagcg ggtcggtctg
    10981 cgtctcggcc agcaagatgc cgtcggtcat cagcttcacg ctggcgccgg gcgacag
//
LOCUS       DAOMVO010000045        10999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1424_length_10999_cov_12.565698, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000045 DAOMVO010000000
VERSION     DAOMVO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_10999_cov_12.565698"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..3172
                     /locus_tag="PKV30_03840"
     CDS             2..3172
                     /locus_tag="PKV30_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL29386.1"
                     /translation="MNVSRLFIQRPVATGLFMLAIVLAGLVGLRFLPLAALPQVDYPT
                     IQVQTLYPGASPDVMSRTVTAPLERQFGQMSGLDRMSSTSAAGVSMVTLQFALNLGMD
                     AAEQQVQAAINAGGSLLPADLPAPPVYAKVNPADAPILQLAISSDSLPLTEVQNMVNT
                     RLALKISQITGVGLVTLAGGQRPAVRVQGNLDALAAMGLGLDSISSAISAGNSSTAKG
                     SFDGPKRSYTINANDQLMTAQDYRELIVAYVGGAPVRLKDVARVSDGSENSRLGAWTA
                     IKNDSGSGSSDGRQGAETPETAALTPAIILNVQRQPGANVIATVDAIKKELPALQQSL
                     PGSVRVQVLSDRTLGIRASVEHVQMELLLAVVMVVLVIFLFLHSLRATAIASIAVPIS
                     LLGTVGAMYLLGYSLDNLSLMALTIATGFVVDDAIVMIENISRHRELGAPPFEAAMKG
                     AGEIGFTIISLTVSLIAVLIPLLFMQEVIGRLFREFAVTLAITILFSAVVSLTLVPMM
                     SARWLEPLDHAQGRIGAWVQRRMDWTVGHYDRGLQWVLAHQPLTLLVALATLAVTVLL
                     YWGMPKDLFPTQSTGQLQGRVQAASDVSYERMAALQRAAAGVVLADPAVQSISSVVGV
                     DAANNTMLSAGRLTVNLRPRGVFSDSEATIMQRLRQSVAQQVAGVALYLQPTQDLTID
                     SDSGPTEFRFDLEGVDTQLVNSWAQRLVARLQSVPEVRHATTDAGAAGLAAMVKIDRD
                     TAARLGVTASTLDNTLYNAFGQRIVSTIFSETNQYRVILEAARDSAATLRTLQELPVK
                     TSSGTTTPLASFAQVVEQPSPLQVLRVSQYPAATVGFDTAPGVSLGAAVQAVRAAATA
                     EGLPESIALHFTGASGAYQKSLSNQVWLIIAAVVCVYIVLGVLYESYVHPLTILSTLP
                     SAGVGALLALWITGHSLDVVGIIGLVLLIGIVKKNAIMMIDFAIEAERGEGKSPQEAI
                     HQAALMRFRPILMTTLAALAAALPLMFSFGDGAELRRPLGIAIFGGLVLSQLLTLFTT
                     PVIYLWFDRLGQRFGGRSGSEAEMAS"
     assembly_gap    3199..3298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3345..>5572
                     /locus_tag="PKV30_03845"
     CDS             3345..>5572
                     /locus_tag="PKV30_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL29387.1"
                     /translation="MNLSRPFIERPIATVLLTIGIALAGIAAYLLLPVARLPSVDFPV
                     ISVSANMPGASPADMARTVATPLERTLGTIAGVNEMTSFSGTGSTRVTLQFDLNRNID
                     SAARDVQAAINAARVDLPANLRSNPTYRKRNPASQPFMILALTSGTRTPGQIYDAVSN
                     IVSQRLLQVEGVGDVELGGGSLPAVRVQLNPFALNELGISGDDVRAAIQANNANRPKG
                     AVEVGGTEDGRALQVMTPAPGLRAADYRNMIIALRNQQEVRLQDVAQVIDSVQDTRTR
                     GMFNGQPAIVVNITQQPDANLIATANAINKLLPQLRAQLPQDINLDVAIDRTGSVRAS
                     VLEVEITLVIAVLLVVVVVGLFLRNVRAAVIPAVATVVSLLGTFGVMYLLGYSLNNLT
                     LMALTVATGFVVDDAIVVLENISRHLEMGQSRKQAALQGAREVGFTVLTISVSLVAVF
                     IPLLFMGGQAGRLFREFAVTLSAAVMISLVISLTTTPMLCAMLLRKQHGDAAPPRQSV
                     ASRLRARLDGWARGGEAVMMKGYASALDWALASPWLVVLVLLVVVGLNGYLFTHITKG
                     FFPEQDNGQMTGGLRADQSISSTALADKLQQAIDIVRADPAVATVVGFAGGGGAGGGF
                     MAAALKPLGQRSGPEGRESTRQVINRLRPQLNQITGLRVFMNPVQELRMGGRSSNSTY
                     QYTLKADNAADLKAWTLKLSARMKQMEPLTDVDDDQSDNGVETFVQVDRNKAAELGVS
                     LA"
     assembly_gap    5573..5672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5673..6520
                     /locus_tag="PKV30_03850"
     CDS             <5673..6520
                     /locus_tag="PKV30_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL29388.1"
                     /translation="SATTGGSSANPALRNASTGQPISVSPANMVPLSAFATFSERSVP
                     TSVWHDGGELSSTLSFNLADGVSLEQGRAAVLQAESDIGMPNNVRGAFAGAAAAAQQT
                     ESQQTVLIVAAIVVIYIVLGILYESLIHPVTVLTTLPSAGFGAALSLLALKMEFSIMA
                     LIGVFLLIGIVKKNAILIIDFALDAERARGLTAVQAVREACLLRFRPILMTTLAAGLG
                     ALPLAIGFGQGSELRQPLGVTIIGGLVASQLLTLLTTPVVYVLLDKLRRRHDGEAHLA
                     RAVAA"
     gene            6543..8021
                     /locus_tag="PKV30_03855"
     CDS             6543..8021
                     /locus_tag="PKV30_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRL29389.1"
                     /translation="MNKYEFDSGLRRTDKRQRPISFKTITAAALLVALCGCGFAPREQ
                     TPVAPLSAAWKTAAPQAGWVNADEARAWQAGLWWELFGDAELSALMQRVNIHNQNLAV
                     AAANVAQAEALLRQQRAGLLPNVGASVGAQRAGGQDRSTTHSGSVGLNVSWAPDLWSQ
                     IGDAVRAQGANVQVSEANLAAARLSAQGSLAAAYFALREQDAEMALLDDIITGYERSM
                     KITQNRYDAGVAARTDVLQAQSTLESARASRVALQMNRELNEHAIALLVGEAPANFSL
                     SASPWVDRVPAVPAGVPSELLLRRPDVAAAERAVAAANARIGVARSAYFPQLTLQGSI
                     GASGSHLLDVISAPKLLWSLGVALAQYVFDGGARTAAVDQALAAHDAATASYRQASLA
                     AMKEVEDELTALLRLAEQIEHARVGADAAARVEQQVMNSYQAGLSAYTNVVTAQATAL
                     SARRSLMQLQLQRQQAAVALVQALGGGWQAPWGAVDAATSPR"
     gene            complement(8039..8188)
                     /locus_tag="PKV30_03860"
     CDS             complement(8039..8188)
                     /locus_tag="PKV30_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29390.1"
                     /translation="MTTYKNAFSTTDHNAITMNMLYLGVVRNGAITFAYPEDARRNLV
                     VQRKG"
     gene            complement(8185..9096)
                     /locus_tag="PKV30_03865"
                     /pseudo
     CDS             complement(8185..9096)
                     /locus_tag="PKV30_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(9353..10297)
                     /locus_tag="PKV30_03870"
     CDS             complement(9353..10297)
                     /locus_tag="PKV30_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL29391.1"
                     /translation="MQFDLTDLRLFVRAADEGALTRAALRQHLSLAAASARIKALEEQ
                     AGVALFHREARGVRLTPAGDAFLHHARGVLRQAEQLRAELQEYGGGLRGHLRVYANTT
                     AVMDFLPELLPAFMAAHPTINIDLQEKPNGDIARGVRDGRADLGIVAGDVDLTGLRAV
                     HFSTDRLVLVVARGHPLARHKSVTFADTLEHDHIGMHSGSTLQAYLSRITEQMGRTLK
                     LRIQLTSFDAMCRMIGGGVGIGVVPESAARRNLAAMRLVQIELTDEWKWRERRVLSRQ
                     SDALPAYAQALVELLCAHFARHEAPGAQRRRNTKRFAK"
     gene            10393..>10999
                     /locus_tag="PKV30_03875"
     CDS             10393..>10999
                     /locus_tag="PKV30_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRL29392.1"
                     /translation="METSNDRALDAAAIAHLQQWVGRSETLTDDIAAAPLRGLSATLD
                     RGDAPPEHGDAVPPLGHWLYFLPQAPQSQIGPDGHPKRGGFLPPVPLPRRMWAGGRLV
                     WEPGNPLRVGDAARKVSRIESVTHKTGRSGEMIFVLVRHEVHNERGLCLTEEHDIVYR
                     AAAQPGEKAAAPTPAETGATWRREIVPDDVLLFRYSALTFNG"
BASE COUNT         1683 a         3628 c         3731 g         1757 t
ORIGIN      
        1 catgaacgtc tcccgcctct tcatccagcg ccccgtcgcc accggcctgt tcatgctggc
       61 catcgtgctg gctgggctgg tgggcttgcg ctttctgcca ctggcggcgc tgccgcaggt
      121 ggattacccg accatccagg tgcagacgct gtaccccggc gccagccccg acgtgatgag
      181 ccgcacggtg acggcgccgc tggagcgcca gttcggccag atgtcggggc tcgaccgcat
      241 gagcagcacc agcgcggcgg gtgtgtcgat ggtcacgctg cagtttgcgc tcaatctggg
      301 catggacgcg gccgagcagc aggtgcaggc ggccatcaac gccggcggct cgctgctgcc
      361 ggccgatttg ccggcgccgc cggtgtacgc caaggtgaat ccggccgacg cgccgatcct
      421 gcaactggcc atcagctccg acagcctgcc gctgaccgag gtgcagaaca tggtcaacac
      481 gcggctggcg ctgaagatca gccagatcac gggcgttggc ctggtcacgc tggcgggcgg
      541 gcagcggccg gcggtgcggg tgcagggcaa cctcgacgcg ctggcggcca tgggcctggg
      601 gctggacagc atcagcagcg ccatcagcgc cggcaattcc agcaccgcca agggcagctt
      661 cgacggcccc aagcgcagct acaccatcaa cgccaacgac cagttgatga cggcgcagga
      721 ttaccgcgag ctgatcgtgg cctacgtggg cggcgcgccg gtgcggctga aagacgtggc
      781 gcgcgtttcc gacggctccg agaacagccg cctgggcgcc tggacggcca tcaaaaacga
      841 tagcggctca ggctcgtctg acgggcgcca gggcgccgaa acgcctgaaa cggcagcgct
      901 cacgccggcc atcatcctga acgtgcagcg ccagcccggc gccaacgtga tcgccacggt
      961 ggacgccatc aagaaggaat tgcccgcgct gcagcaaagc ctgccgggca gcgtgcgggt
     1021 gcaggtgctg tccgatcgca cgctgggcat tcgcgcctcg gtcgagcacg tgcagatgga
     1081 gctgctgctg gcggtggtga tggtggtgct ggtgatcttc ctgttcttgc acagcctgcg
     1141 cgccacggcg atcgcgtcga ttgcggtgcc gatctcgctg ctgggcacgg tgggcgcgat
     1201 gtacctgctg ggctactcgc tcgacaatct gagcctgatg gcgctgacga ttgccaccgg
     1261 cttcgtggtc gatgacgcca tcgtcatgat cgagaacatc tcgcgccacc gcgagctggg
     1321 cgcgccgccg ttcgaggcgg cgatgaaggg cgcgggcgag atcggcttca ccatcatctc
     1381 gctcacggtg tcgctgatcg cggtgctgat tccgcttttg ttcatgcagg aggtgattgg
     1441 ccgcttgttc cgcgaattcg cggtcacgct ggcgatcacc atcttgtttt cggccgtggt
     1501 ctcgctcacg ctggtgccca tgatgtcagc gcgctggctg gagccgctgg accacgcgca
     1561 gggccgcatc ggcgcctggg tgcagcggcg catggactgg acggtgggcc actacgaccg
     1621 cggcctgcaa tgggtgctgg cgcatcagcc gctgacgctg ctggtggcgc tggccacgct
     1681 ggcggtgacg gtgctgctgt actggggcat gcccaaggac ttgttcccga cgcagagcac
     1741 cggccagttg cagggccgcg tgcaggcggc cagtgatgtg tcttacgaac gcatggccgc
     1801 gctgcagcgg gcggcggccg gcgtggtgct ggccgacccg gcggtgcagt cgatcagctc
     1861 ggtggtcggc gtcgatgcgg ccaacaacac catgctgtcg gccgggcgcc tgacggtgaa
     1921 cctgcgcccg cgcggcgtgt tcagcgattc cgaagccacc atcatgcagc gcctgcggca
     1981 aagcgtggcc cagcaggtgg ccggcgtggc gctgtacctg cagcccacgc aagacctgac
     2041 catcgacagc gacagcggcc cgaccgagtt ccgcttcgac ctggaaggcg ttgacacgca
     2101 actggtcaac agctgggcgc agcgcctggt cgcgcgcctg caaagcgtgc ccgaggtgcg
     2161 ccacgccacc accgacgccg gcgccgcagg gctggccgcc atggtgaaga tcgaccgcga
     2221 caccgccgcg cgcctgggcg tgacggccag cacgctcgac aacaccctct acaacgcctt
     2281 tggccagcgc attgtctcga ccatcttcag cgagaccaac cagtaccgcg tgattctgga
     2341 agccgcgcgc gacagcgccg ccaccctgcg cacgctgcag gagctgccgg tgaaaaccag
     2401 cagcggcacc accacgccgc tggccagctt tgcgcaggtg gtcgagcagc cgtcgccgct
     2461 gcaggtgctg cgcgtgtcgc agtacccggc cgccacggtg ggcttcgata ccgcgcccgg
     2521 cgtgtcattg ggcgccgcgg tgcaggcggt gcgcgcggcg gccaccgccg aggggctgcc
     2581 ggaatcgatt gcgctgcatt tcaccggcgc ctcgggcgcg taccagaagt cgctgtcgaa
     2641 ccaggtgtgg ctgatcattg ccgccgtggt gtgcgtgtac atcgtgctgg gcgtgctgta
     2701 cgaaagctat gtgcacccac tgacgatctt gtccacgctg ccctcggcgg gggtcggcgc
     2761 gctgctggcc ttgtggatca ccgggcattc gttggacgtg gtgggcatca tcggcctggt
     2821 gctgctgatc ggcatcgtga agaagaacgc gatcatgatg atcgacttcg ccatcgaggc
     2881 cgaaaggggc gagggcaagt cgccgcaaga ggcgattcac caggcggcgc tgatgcgctt
     2941 tcggccgatt ctgatgacca cgctggcggc gctggcggcc gcgctgccct tgatgttcag
     3001 ctttggcgat ggggcggagt tgcggcggcc attggggatc gcgatttttg gcgggctggt
     3061 gttgtcgcag ttgctgacgc tgttcacgac gccggtgatt tatttgtggt tcgatcggtt
     3121 ggggcagagg tttggggggc ggtccgggtc tgaggcggag atggcgtcat gagggctgtt
     3181 ttgggctctg gcgcttgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     3301 ccctcacccc aaccctctcc ccagaggggc gagggagtga agagatgaac ctctcccgcc
     3361 ccttcatcga acgccccatc gccaccgtcc tgctgaccat cggcatcgcg ctggccggca
     3421 tcgccgccta cctgctgctg ccggtcgcgc gcctgccctc ggtcgacttc cccgtcatct
     3481 ccgtcagcgc caacatgccg ggtgcctcgc ccgccgacat ggcgcgcacc gtcgccacgc
     3541 cgctggagcg cacgctcggc accattgccg gcgtcaacga gatgacctcg ttcagtggca
     3601 ccggctcgac ccgtgtcacg ctgcagttcg acctcaaccg caacatcgac agcgccgcgc
     3661 gcgacgtgca ggctgccatc aacgccgcgc gcgtcgatct gccggccaac ctgcgcagca
     3721 acccgactta ccgcaaacgc aacccggcgt cgcagccgtt catgatcctg gcgctcacct
     3781 ccgggacgcg cacgccgggg cagatctacg acgcggtcag caacatcgtc agccagcggc
     3841 tgctgcaggt cgagggcgtg ggcgatgtgg agctgggcgg cggctcgctg cccgcggtgc
     3901 gggtgcagct gaacccgttt gcgctcaatg aactgggcat cagcggcgac gacgttcgcg
     3961 ccgccattca agccaacaac gccaaccggc ccaagggcgc ggtcgaagtg ggcggcactg
     4021 aagacgggcg cgcgctgcag gtgatgacgc ccgcgccggg cctgcgcgcg gcggactacc
     4081 gcaacatgat catcgccctg cgcaaccagc aagaggtgcg gctgcaggac gtggcgcagg
     4141 tgatcgacag cgtgcaggac acgcgcacgc gcggcatgtt caacggccag ccggccatcg
     4201 tggtcaacat cacccaacag cccgacgcca acctgatcgc caccgccaac gccatcaaca
     4261 aactgctgcc gcaattgcgc gcgcaactgc cgcaggacat caatctcgac gtcgccatcg
     4321 accgcaccgg cagcgtgcgc gcctcggtgc tcgaggtcga gatcacgctg gtcatcgccg
     4381 tgctgctggt cgtcgtggtc gtcgggctgt tcctgcgcaa cgtgcgggcg gcggtgattc
     4441 cggcggtggc cacggtggtg tcgctgctgg gcaccttcgg cgtcatgtac ctgctgggct
     4501 attcgctcaa caacctgacg ctgatggcgc tcaccgtggc gaccggcttc gtggtggacg
     4561 acgccatcgt ggtgctcgaa aacatctcgc gccacctgga gatgggccag tcgcgcaagc
     4621 aggcggcgct gcagggcgcg cgcgaggtcg gcttcactgt gctgaccatc agcgtgtcgc
     4681 tggtggcggt gttcattccg ctcttgttca tgggcgggca ggcggggcgg ctgtttcgcg
     4741 aatttgcggt cacgctgtcg gcggcggtga tgatttcgct ggtcatctcg ctcaccacca
     4801 cgccgatgct gtgcgccatg ctgctgcgca agcagcacgg cgatgcggcg ccgccgcgcc
     4861 agagcgtggc cagccgcctg cgcgcgcgcc tggacggctg ggcgcgcggc ggcgaggcgg
     4921 tcatgatgaa gggctatgcc agcgcgctgg attgggcgct ggcatcgccc tggctggtgg
     4981 tgctggtgct gctggtggtg gtggggctga acggctatct gttcacgcac attaccaagg
     5041 gcttcttccc cgagcaggac aacggccaga tgacgggcgg cctgcgcgcc gaccagagca
     5101 tttcgtccac cgcgctggcc gacaagctgc agcaggccat cgacatcgtg cgcgccgatc
     5161 cggcggtggc caccgtggtc ggctttgcgg gcggcggcgg ggccggcggc ggtttcatgg
     5221 cggcggcgct caagccactc ggccagcgct cgggccccga ggggcgcgag agcacgcgcc
     5281 aggtcatcaa ccgcctgcgc ccgcaactca accagatcac cgggctgcgc gtgttcatga
     5341 acccggtgca agagctgcgc atgggcgggc gatccagcaa cagcacctac caatacacgc
     5401 tgaaggccga caacgcggcc gacctgaagg cctggacgct caagctgtcg gcgcgcatga
     5461 agcagatgga gccgctcacc gacgtcgacg acgaccagag cgacaacggc gtggaaacct
     5521 tcgtgcaggt cgatcgcaac aaggcggccg agctcggcgt gtcgctggcc agnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccagcgcc accaccggcg gctcgtcggc
     5701 caacccggcg ctgcgcaacg cgtccaccgg gcagccgata tcggtctcgc ccgccaacat
     5761 ggtgccgctg tcggcctttg ccactttcag cgaacgcagc gtgcccacca gcgtgtggca
     5821 cgacggcggc gagctgtcca gcacgctgtc gttcaacctg gccgacggcg tgtcgctgga
     5881 gcaggggcgc gccgccgtgc tgcaggccga aagcgacatc ggcatgccca acaacgtgcg
     5941 cggcgccttt gccggcgcgg ccgccgcggc gcagcaaacc gagtcccagc agacggtgct
     6001 gatcgtcgcc gccatcgtgg tgatctacat cgtgctcggc atcctgtacg aaagcctgat
     6061 ccaccccgtg acggtgctca ccaccttgcc cagcgccggc tttggcgccg cgctatcgct
     6121 gctggcgctg aagatggagt tttccatcat ggcgctgatc ggcgtgtttt tgctgatcgg
     6181 catcgtgaag aaaaacgcca tcctgatcat cgacttcgcg ctcgacgccg agcgcgcgcg
     6241 cggcctgacc gcggtgcagg cggtgcgcga ggcgtgcctg ctgcgctttc ggccgatttt
     6301 gatgaccacg ctggccgccg gcctgggcgc gctgccgctg gccatcggtt tcggccaggg
     6361 gtctgaattg cgccagccgc tcggcgtgac catcatcggc ggcctggtgg ccagccagtt
     6421 gctgacgctg ctgacgacgc cggtggtcta cgtgctgctc gacaaactgc ggcgccgcca
     6481 cgacggcgag gcgcacctgg cgcgcgcggt cgcggcatga acaagggagt caagggcagt
     6541 tgatgaataa atacgaattt gatagcggct tgcgcaggac cgacaagcgc cagcggccga
     6601 tttcattcaa aaccatcacc gcagcggcat tgctggtggc gctgtgcggc tgcggctttg
     6661 caccgcgcga gcagacgccc gtcgcgccgc tgtcggccgc gtggaagacc gcggcgccgc
     6721 aggcgggctg ggtcaacgcc gacgaagcac gcgcctggca ggccggcctc tggtgggagc
     6781 tgtttggcga cgccgagctg tcggcgctga tgcagcgcgt gaacatccac aaccagaacc
     6841 tggccgtggc cgccgccaac gtggcgcagg ccgaggcgct gctgcgccag cagcgcgctg
     6901 gcctgctgcc gaacgtcggc gcgtcggttg gcgcgcagcg cgccggcggg caggacagga
     6961 gcaccacgca ttccggctcg gtcggcctca acgtcagctg ggcgcccgat ctgtggagcc
     7021 agatcggcga cgcggtgcgc gcgcagggcg ccaacgtgca ggtgagcgaa gccaacctgg
     7081 cggcggcgcg cctgtcggcc caggggagcc tcgccgccgc ctactttgcg ctgcgcgagc
     7141 aggacgccga gatggcgctg ctggacgaca tcatcaccgg ctacgaacgc agcatgaaga
     7201 tcacgcagaa ccgctacgac gctggcgtgg ccgcgcgcac cgacgtgctg caggcgcaaa
     7261 gcacgctgga aagcgcgcgc gccagccgcg tggcgctgca gatgaaccgc gagctgaacg
     7321 agcacgccat tgcgctgctg gtgggtgagg cgccggccaa tttttcgctg agcgcctcgc
     7381 cctgggtcga ccgcgtgccc gccgtgccgg ccggcgtgcc gtctgaactg ctgctgcgcc
     7441 ggcccgacgt ggccgccgcc gagcgcgccg tggccgccgc caatgcgcgc atcggcgtgg
     7501 cgcgcagcgc ctactttccg cagctcacgc tgcaaggcag catcggcgcc agcggcagcc
     7561 acctgctgga cgtgatttcg gcacccaaac tgctgtggtc gctgggcgtg gcgctggcgc
     7621 agtacgtgtt tgacggcggc gcacgcaccg ccgcggtcga tcaggccctg gccgcgcacg
     7681 acgccgccac cgccagctat cggcaagcgt cgctggccgc catgaaagag gtggaagacg
     7741 aactcaccgc gctgctgcgc ctggccgagc agatcgaaca cgcccgtgtc ggcgccgacg
     7801 ccgccgcgcg cgtcgagcag caggtgatga acagttacca ggctggcctg tccgcctaca
     7861 ccaacgtggt gacggcacag gcgacggcac tcagcgcgcg ccgctcgctg atgcagctgc
     7921 aactgcagcg ccagcaagcg gcggtggcgc tggtgcaggc gctgggcggc ggctggcagg
     7981 cgccgtgggg tgctgtggac gccgcaacgt cgcctcgttg acggctggcg ccgctgcctt
     8041 accccttgcg ctgaaccacc aggttccgcc gcgcgtcctc cggataggcg aaggtgattg
     8101 cgccgttgcg gaccacaccc aggtacagca tgttcatggt aatggcgttg tggtccgtcg
     8161 tgctgaaggc gttcttgtag gtggtcacca cgccgtagta gggcggtcgt tggtttccag
     8221 cgcgtgcttg atcgccgggc cgtcgatggt cttgttcttg atcgtgaaca gcgcataggc
     8281 cagcagatag cccgcgtcgt agccttgtgc ggcagccacc ggcacggtga tgcgctccac
     8341 cttgtaggca cggcggaagt tggccaggaa ggttgagcgg cgttcgttgc tcggctcggc
     8401 aatgaaggtc tgtgccatca gcgaaccttc ggccgcgctc ttggcgttgt cgatgtagaa
     8461 cggaaaggac agcggccagg tgcctatctg ggtcaccttc cagcccagcg cctcgcgggc
     8521 gcggccgatc agcgcgtttt cgggccccac ggtgatcgtg aacactgtct gcgcgccggc
     8581 gtcacgcgcc tctttcacgg cgtcggacaa gtccttgacg cccacatcaa agcgctagac
     8641 gtacgccggc ttgagcttgt gcgcctccag cgcggccatg aagtccttga accccgcctc
     8701 gccgtagccg gtggtgtcgg caaaaatggc gatcttcgtc cagccgcgct tgacggcatc
     8761 gttgacgaca aagggcacct gcaggttgtc ggtggcggac gactggaaga tgtagctttc
     8821 ggcggtgggg tatttcttgg tgatcggcga tccggtcgcg cagggcacca gcagcggcac
     8881 cttgttcttc tggaactcgt ccagcgcctt catcgcgttg ccggtgttgc aaaagccgat
     8941 ggtggcgacc acgccttcct tgatcagttc ctgcgcggca gcgagcgcac cgtccggcgt
     9001 gctcttgtcg tccttgacga cgacctcgaa cttgcgtccc acgtaaccgc cgaggtcgtt
     9061 gatctccttg atggccagtt ccgcgccacg cttcatcgag gtgccgaaat cggcagagcc
     9121 gccactgaaa ggcccgacaa ggccgatctt gatcgcgggt tgttgcgcgc tggcggacgc
     9181 tgccatcagc gcaagcgacg cgccgaccaa actcagcacg cccgatctgt tggttctcgt
     9241 gaacacggca tgagtctcct gaaaaaatct ggctggatta gaaccgcgat tggcgtgccc
     9301 atcggcatag tggaaacccg gaacgcccac gcacgctcac tggcaatgcc aactatttgg
     9361 cgaatcgctt cgtgttgcgc cggcgctgcg caccaggcgc ttcgtgccgg gcaaagtgcg
     9421 cgcacagcag ctcgaccagc gcctgcgcat acgccggcag ggcatcgctc tggcgcgaga
     9481 gcacgcgccg ctcgcgccac ttccactcat cggtcagctc gatctgcacc agcctcatcg
     9541 ccgccagatt gcgccgcgcc gcgctctcgg gcaccacgcc gatgccgacg ccgccgccga
     9601 tcatgcggca catggcgtcg aagctggtca gctggatgcg cagcttgagc gtgcgtccca
     9661 tctgctcggt gatgcggctc aggtaggcct gcagcgtgct gccgctgtgc atgccgatgt
     9721 ggtcgtgctc cagcgtgtcg gcaaatgtca ccgacttgtg gcgcgccagc gggtgaccgc
     9781 gcgccaccac cagcaccagc cggtcggtcg agaagtggac cgcgcgcagc ccggtcaggt
     9841 cgacatcgcc ggcgacgatc cccaagtcgg cgcggccgtc gcgcacgccg cgcgcgatgt
     9901 caccgttcgg tttttcctga aggtcgatgt tgatcgtcgg gtgcgccgcc atgaaggcgg
     9961 gcagcagctc gggcaggaaa tccatcaccg ccgtggtgtt ggcgtacacg cgcaagtgcc
    10021 cgcgcaggcc gccgccgtat tcctgcagtt cggcgcgcaa ctgctcggcc tggcgcagca
    10081 cgccgcgcgc gtggtgcaga aaggcatcgc ccgccggcgt gaggcgcacg ccacgcgctt
    10141 cgcggtggaa cagtgccacg ccggcctgtt cttccagcgc cttgatgcgc gcgctggcgg
    10201 cggccagcga caggtgctgg cgcaaggcgg cgcgggtgag ggcgccttcg tcggcggcgc
    10261 gcacgaacag gcgcaggtcg gtcagatcga attgcatggt ggttggcgca gtggcaagcc
    10321 ttcggattat ccgaaacctt gttctcgaaa tcaccgatgg tgctatggcc cggcgcgggc
    10381 caacatcggg ccatggagac aagcaacgac cgcgcgctgg acgccgccgc catcgcccac
    10441 ctgcagcaat gggtggggcg cagcgaaaca ctgaccgacg acattgccgc cgcgccgctg
    10501 cgcggcctgt cggccacgct ggacaggggc gacgcgccgc ccgagcatgg cgacgccgtg
    10561 ccgccgctcg gccactggct gtactttctg ccgcaggcgc cgcaaagcca gatcgggccg
    10621 gacggccacc ccaagcgcgg cggctttctg ccgcccgtgc cgctgccgcg ccgcatgtgg
    10681 gctggtggcc gcctggtgtg ggagccgggc aacccgctgc gtgtgggcga tgcggcgcgc
    10741 aaggtctcgc gcatcgaatc ggtgacgcac aagaccgggc gcagcggcga aatgatcttt
    10801 gtgctggtgc gccatgaagt gcacaacgag cgcggcctgt gcctgaccga agagcacgac
    10861 atcgtttacc gcgcggcagc gcaaccgggc gaaaaggccg ctgcgcccac gccggccgaa
    10921 acgggggcca cgtggcggcg cgaaatcgtg cccgacgacg tgctgctgtt ccgctacagc
    10981 gcgctcacct tcaacggcc
//
LOCUS       DAOMVO010000046        10883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1450_length_10883_cov_18.115257, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000046 DAOMVO010000000
VERSION     DAOMVO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_10883_cov_18.115257"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKV30_03880"
     CDS             complement(<1..533)
                     /locus_tag="PKV30_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HRL29393.1"
                     /translation="MQAAWMIAASLFFALMSVCIKYASPYFGPLEIVCYRGAIGIVFM
                     WALARSRGVSLKTSVPMMHVWRNVVGVAALVAWFYAIAHLPLATAMTLNYMSGVWVAA
                     FLIGGTLLLGRIADIRRQGPLVLAVMAGFAGVVMMLRPTLDQNQLFAGLIGLLSGLMS
                     ALAYIQVAALGRVGEPEA"
     gene            complement(651..1787)
                     /locus_tag="PKV30_03885"
     CDS             complement(651..1787)
                     /locus_tag="PKV30_03885"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRL29394.1"
                     /translation="MSDLSLQLQQAASQLPVTSYFDQALFEREKRLIFESGPRYVGHS
                     LAVPNPGDYFALPQEAEGRALARNPQGGVELISNVCRHRQAVMLKGRGSLQTQGKGHA
                     GGNIVCPLHRWTYSPKGELLGAPHFKHDPCLNLNNYSLREWNGLLFEDNGRDIAADLG
                     HMGVAKDLSFEGYAFDHVELHECNYNWKTFIEVYLEDYHVGPFHPGLGNFVTCDDLHW
                     QFGRDYSVQTVGVASSFGKPGSDVYKKWHEVLLNYQGGEKPTRGAIWLTYYPHIMVEW
                     YPHVLTVSTLHPISAGKTLNMVEFYYPEEIVAFEREFVEAQRAAYMETCIEDDEIAER
                     MDAGRKALMLRGDNEVGPYQSPMEDGMQHFHEWYRRAMDTTIVA"
     gene            2016..2255
                     /gene="xseB"
                     /locus_tag="PKV30_03890"
     CDS             2016..2255
                     /gene="xseB"
                     /locus_tag="PKV30_03890"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRL29395.1"
                     /translation="MSRKSATSPTQPANYGDAVQQLERLIDGLESGQLPLEELLGQYQ
                     RGTELLRYCRDRLQAVEDQVKVLGEGGALQPWTAE"
     gene            2252..3145
                     /locus_tag="PKV30_03895"
     CDS             2252..3145
                     /locus_tag="PKV30_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRL29396.1"
                     /translation="MSAADFSAWSAKQLVQVEQALSAWVPADAPAGLGDAMRYAVLDG
                     GKRLRPLLVLAASESVQGNPHAALRAACAVELIHAYSLVHDDMPCMDNDVLRRGKPTV
                     HVQFGEARALLAGDALQALAFELLTPEGADVPPAMQAELCRLLARAAGAQGMAGGQAI
                     DLASVGKPLSETELRGMHERKTGALLQASVRMGAACGSAVTPAARAALDDFGAVIGLA
                     FQVVDDILDVTADSAELGKTAGKDAAEDKPTYVSLLGLPAAQALADALCSQAHAALEA
                     SGLPGVERLRALADLVVKRRN"
     gene            3147..5051
                     /gene="dxs"
                     /locus_tag="PKV30_03900"
     CDS             3147..5051
                     /gene="dxs"
                     /locus_tag="PKV30_03900"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRL29397.1"
                     /translation="MDDKLLSAIQDPGDLRRLPRAQLKGLADEVRECVLQNVSRTGGH
                     LSSNLGTVELTIALHYVFNTPHDRLVWDVGHQTYPHKILTGRRERMPTLRQLGGISGF
                     PRREESEYDTFGTAHSSTSISAALGMAVAARQKGEDRNAVAIIGDGAMTAGMAFEALN
                     NAGVERDARLLVVLNDNDMSISPPVGALNRYLAQLMSGNFYAAAKNVGKTVLGPVPPL
                     FELAKRIEQGAKGMVVPATLFEKFGFNYIGPIDGHDLDSLIPTLENIRDRMAKQEGPQ
                     FLHVVTKKGQGYKLAEADPVAYHGPGKFDPAVGLTPPATPPKPTFTQVFGQWLCDMAE
                     HDKRLVAITPAMREGSGMVEFHRRFPDRYYDVGIAEQHAVTFAAGLACEGLKPVVAIY
                     STFLQRAYDQLIHDVAIQKLPMVLALDRAGLVGADGATHAGAFDIAYLRCVPNVSVAC
                     PADERECRQLLSTAFAQDHAVAVRYPRGAGVGVQPLADLSELPFGKGAVRRQGQRIAM
                     LAFGSLLYPALQAAERLGATVVNMRWAKPLDTELLLQIASSHEALVTIEEGVVMGGAG
                     SAVAEALAAAQIAKPLLQLGLPDAFIEHGDPARLLALQGLDAVGIEASVRGRFGDLIG
                     PAAPVLKVVG"
     gene            5165..6247
                     /gene="dctP"
                     /locus_tag="PKV30_03905"
     CDS             5165..6247
                     /gene="dctP"
                     /locus_tag="PKV30_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRL29398.1"
                     /translation="MDRRSLIKRTGIAGVLAAGVAPAVHAQPAVRWRLASSFPKSLDT
                     IFGSAEKMSQTVKALSGGKFEVSVHPAGELMPAFGVVDALEKDTIEMAQSAAYYFAGK
                     DPIFSMSCAIPFGLTTLQMAAWKLHGNGGKLLNEFFAKYNFRTMSAGNTTTQMGGWYR
                     KEIKSVADLKGLKMRLGGGIFGEAMAKLGVVAQSLPAGDIYQALEKGTLDAAEFVGPY
                     DDEKLGFHKVAPYYYYPGWFEGSANLEFFINNKKYEALSAENKAILQAACAVAAQDMT
                     AKYGAQNPPALKRLVGGGTKLTLFPKAVMDAAWKATQEVCAAHSAKSPEFKKIYDDMR
                     NFQRDQILWNRFSEYPFNQYMNTLKI"
     gene            complement(6392..8260)
                     /locus_tag="PKV30_03910"
     CDS             complement(6392..8260)
                     /locus_tag="PKV30_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRL29399.1"
                     /translation="MEFFIANYAAFMFAGLICFLLLGFPVAFSLGACGLFFGLLGIEL
                     NVFPASIIAWLPQRLLGIMANDTLLAVPFFTFMGLVLERSGMAEDLLDTVGQVFGPMR
                     GGLALAVIFVGALLAATTGVVAASVISMGLISLPIMMRYGYDRRLASGVIAASGTLAQ
                     IIPPSLVLIIMADQLGRSVGDMYKGAFVPAFMLVGMYVLYVVLLAIFKPKMVPALPVE
                     ARVFREPNGSSGYTSLLVLTVVSVLVAVFLARHMAEVHTWWQGTLVESVPTDEKIVVA
                     MCGGVTVAWLIAFINRVTRMGLLSRLAERVTFVLIPPLLLIFLVLGTIFLGVATPTEG
                     GAMGAMGALIMAMMRGRMSFALLNQALGSTTRLASFVMFILVGATVFSMVFQAADGHK
                     WVEHLLDDLPGGQVGFLILVNVLIFVLAFFLDFFELSFIVVPLLAPVADKLGIDLIWF
                     GVLLAVNMQTSFMHPPFGFALFYLRSVAPEKPYVDKVTGKVMQPVTTMEIYKGAIPFL
                     VLQLVMVAVLIAFPSLVTGSLDKKVDVDMDAVGAQMRDSLGAPGAGDAATSENLWGND
                     ASGSEAPAGAASEADGAVAPADPASAASAEESSAAPTPGGYGSDDPLKSLQESIKK"
     gene            complement(8279..8893)
                     /locus_tag="PKV30_03915"
     CDS             complement(8279..8893)
                     /locus_tag="PKV30_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRL29400.1"
                     /translation="MQALLKLSRAIDWLNATVGKYAIWLIFAATVISALNAVVRKAFN
                     FSSNAYLEVQWYLFAWSFLIAAGYTLLHREHVRIDVLNSRLPKRAQVWIDIIGFAFFL
                     TPLCIVVLYLGIPMLISKFQSGEVSPNAGGLVRWPVWLALPLGFGLLLLQGWSELIKR
                     VAFLRGVGPDPMGRLTDKSAEEELAEALRRETSPDAAPAADTTR"
     gene            complement(9001..10128)
                     /locus_tag="PKV30_03920"
     CDS             complement(9001..10128)
                     /locus_tag="PKV30_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRL29401.1"
                     /translation="MQKIGLSYSLGTDGGGPLIRNPLLDLLQAVRSSGSISSAASALG
                     LSYRHVWGELRRWEQELGQPLLHWEKGRRARLSEFGDKLLWSERQAQARLAPQIEALR
                     AELERTFAVAFDDAAHVLTLYASHDDALVALRQHAASLPGTPRLHLDIRFTGSVDAIA
                     ALNEGRCIMGGFHTLRTPAADSLAARTYKPLLQPGLHKLIGFATRTQGLMVRPGNPHR
                     IQGLADLNKAGVRFVNRALGTGTRVLLDELLAAEDLAAADVRGYHDVEPSHAAVAQAV
                     AAGGADAGLGIEAAARARGLDFVPLIAEDYHLVCLKEALDEAPVAALRALLASAGWAH
                     ELNALPGYQASHAGEVRSMASVLPWWQFKQPKRRAGMPARR"
     gene            10264..>10883
                     /locus_tag="PKV30_03925"
     CDS             10264..>10883
                     /locus_tag="PKV30_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL29402.1"
                     /translation="MNTFVDSGRTALELITSLDPALWTVVARSLAVSATACALGYGVG
                     VVAGAWLGVTRFRGRNVVLTLLNTLLALPSVVVGLLVYLLLSRSGPLGFLGWMFSFKA
                     MVVAQVVLVLPVVTALTRQAVEDADLQHGEQLASLGAGRFMRGLLLAWDERYALLTVL
                     MAAFGRAISEVGAVMIVGGNIDGFTRVMTTAIALETSKGDLPLALGL"
BASE COUNT         1939 a         3519 c         3639 g         1786 t
ORIGIN      
        1 gcttcgggct cgcccacgcg gcccagcgcc gccacctgga tataggccag cgccgacatc
       61 aatccggaca gcaggccgat gaggccggcg aacaactggt tctggtccag cgtggggcgc
      121 agcatcatca ccacgccagc aaagccggcc attacggcca gcaccagcgg gccctggcgg
      181 cggatgtcgg cgatgcggcc cagcagcagc gtgccgccga tcaggaaggc ggcaacccac
      241 acgccgctca tgtagttgag cgtcatggcg gtggccagcg gcaggtgggc gatggcgtag
      301 aaccaggcca ccagggcggc cacgccgacg acgttgcgcc acacgtgcat catgggcaca
      361 ctggtcttca ggctgacacc gcgcgagcgc gcaagcgccc acatgaacac gatgccgatg
      421 gcgccgcggt agcagacgat ttcaagcggg ccgaagtagg gcgatgcgta cttgatgcac
      481 acgctcatca gtgcaaaaaa gagcgaggcg gcaatcatcc aggcggcttg catgggccgc
      541 gattatgaat gaaatcaggc tctagcgctt ttgtagcaag cgcaagcagc tacaaaaaaa
      601 agagcgcccg ctgttttttg gcagcgggcg ctggggagga aattgcaccg tcaggcgacg
      661 atggttgtgt ccatggctct tcgataccac tcgtggaagt gctgcatgcc gtcttccatc
      721 gggctctggt aggggccgac ctcgttgtcg ccgcgcagca tcaacgcctt gcggccggcg
      781 tccatgcgtt cggcgatctc gtcgtcctcg atgcaggttt ccatgtaggc ggcgcgctgt
      841 gcctcgacga attcgcgctc gaaggcgacg atctcctcgg ggtagtagaa ctccaccatg
      901 ttcagcgttt tgcccgcgct gatggggtgc agcgtgctga cggtgagcac atgcgggtac
      961 cactcgacca tgatgtgcgg gtagtaggtc agccagatgg cgccgcgcgt gggcttttcg
     1021 ccgccctggt agttcagcag cacctcgtgc cacttcttgt agacgtcgga gcccggcttg
     1081 ccaaagctgc tggccacgcc cacggtctgc accgagtagt cgcggccaaa ctgccagtgc
     1141 aggtcgtcgc aggtcacaaa gttgcccaaa ccggggtgga acgggccgac gtggtagtcc
     1201 tccagataga cctcgatgaa ggtcttccag ttgtagttgc actcgtgcag ttcgacgtgg
     1261 tcgaacgcgt agccttcgaa cgataaatcc ttggccacac ccatgtggcc taagtcggca
     1321 gcgatgtcgc ggccgttgtc ttcgaacagc aggccgttcc actcgcgcaa gctatagttg
     1381 ttcaggttca ggcaagggtc gtgcttgaag tgcggcgcgc ccagcaactc gcccttgggc
     1441 gagtacgtcc agcggtgcaa cgggcacacg atattgccgc cggcgtggcc cttgccttgc
     1501 gtttgcagcg agccacggcc cttgagcatc acggcctgac ggtggcggca gacgttggaa
     1561 atcagttcga cgccgccctg agggttgcgc gccagcgcgc ggccctcggc ctcttgcggc
     1621 agggcaaagt aatcaccagg gttgggcacg gcgagcgaat gcccgacgta gcgcggcccg
     1681 gactcgaaga tgagccgctt ttcgcgctca aacagcgcct ggtcgaaata ggaagtgacg
     1741 ggaagttgac ttgccgcctg ctgtaattga agacttaaat cagacatggt gttccgacct
     1801 aaagactccc cctatgaagg ggcaggaagt aagcgcttgg aatccgagga tatggcgctt
     1861 gcggacccag ggcgtttgcg ccaaaggaac ttttgattct aactggatgc ggccgatcgc
     1921 gtggatttca aggttgtagg cgcaggcgcc gatccgtgca tagttggacg tatgtggtcg
     1981 gtgcccaact aaaatacggc cctttgcccg cctgcatgtc acgcaagtcc gccaccagtc
     2041 ccacgcagcc cgccaactat ggcgacgcgg tgcaacagct tgagcgcctg atcgacgggc
     2101 tggaatctgg tcaattgccg ctggaagaac tgctgggcca gtatcagcgc ggcaccgagt
     2161 tgctgcgcta ttgccgtgat cggctgcagg cggtcgaaga tcaagtgaag gtgctgggtg
     2221 aaggcggcgc gctccaaccg tggacggccg aatgagcgcc gctgatttct ccgcttggtc
     2281 ggcaaagcag ttggtgcagg ttgaacaggc cttgtcggcc tgggtgccgg cagatgcacc
     2341 ggccggcctg ggcgacgcca tgcgatatgc cgtcctcgat ggcggcaagc gtttgcggcc
     2401 gctgttggtg ttggcggcca gcgaatcggt gcaaggcaac ccccatgcgg cgctgcgggc
     2461 tgcctgcgcg gtcgaattga ttcacgccta ttcgctggtg cacgacgaca tgccgtgcat
     2521 ggacaacgac gtgctgcgcc gtggcaagcc gacggtgcac gtgcagtttg gcgaggcgcg
     2581 cgccttgctg gccggcgatg cgctgcaggc gctggcgttc gagttgctga cgcctgaggg
     2641 cgctgatgtg ccaccggcca tgcaggccga gttgtgtcgc ctgctggccc gcgcggctgg
     2701 cgcgcagggc atggcgggcg gccaggcgat cgacctggcc agcgtcggca aacccttgag
     2761 cgaaaccgag ttgcgcggca tgcacgagcg caagaccggc gcgctcctgc aagcgagcgt
     2821 gcgcatgggc gctgcctgcg ggagcgccgt gacacctgcc gcgcgcgcgg cgctcgatga
     2881 cttcggcgcc gtcatcgggc tggcgtttca ggtcgtagac gacattctgg atgtcacggc
     2941 cgattccgcc gagctcggca agaccgccgg caaggacgcc gcggaagaca agcccaccta
     3001 cgtatcgttg ctgggcctgc ctgcagcgca ggcgctggct gatgcgctct gcagccaggc
     3061 ccatgcggcc cttgaagcga gcggcctgcc cggtgtcgaa cgcctgcgtg cgctggccga
     3121 cctggtggtc aaacgcagga attgacatgg acgacaagct actctccgct atccaagacc
     3181 ccggcgacct gcgccgcctg ccgcgcgcgc aactcaaagg cctggccgac gaggtgcgcg
     3241 agtgtgtgct gcagaacgtg tcgcgcaccg gcggccacct cagctccaac ctcggcacgg
     3301 tggaactcac catcgccttg cactacgtct tcaacacgcc gcacgaccgg ctggtgtggg
     3361 atgtgggcca ccaaacctat ccgcacaaga tcctgaccgg tcggcgcgag cgcatgccca
     3421 cgctgcgcca actgggcggc atctcgggct ttccgcgtcg tgaagaaagc gaatacgaca
     3481 cctttggcac tgcccattcc tcgaccagca tttccgccgc ccttggcatg gcggtggcgg
     3541 cgcggcaaaa gggcgaagac cgcaacgcgg tggccatcat cggcgatggc gccatgacgg
     3601 ccggcatggc gttcgaggcg ctcaacaacg ccggcgtcga gcgcgatgcg cgcctgctgg
     3661 tggtgctcaa cgacaacgac atgtcgatca gcccgccggt cggcgcgctc aaccgctatc
     3721 tggcgcagct gatgagcggc aatttctacg ccgctgccaa gaacgttggc aagacggtac
     3781 tcggcccggt gccgccgttg ttcgagttgg ccaagcgcat cgagcagggt gccaagggca
     3841 tggtggtgcc ggccacgctg ttcgagaagt ttggcttcaa ctacatcggg cccattgacg
     3901 ggcacgacct ggattcgctc atccccacgc tggagaacat ccgcgaccgc atggccaagc
     3961 aggaggggcc gcagtttctg cacgtggtca ccaagaaagg ccagggctac aagctggccg
     4021 aagccgatcc ggtggcctac cacggcccgg gcaagttcga tccggcggtg ggcctgacgc
     4081 cgcccgccac gccgcccaag cccaccttta cgcaggtgtt tgggcaatgg ctgtgtgaca
     4141 tggccgagca cgacaagcgc ctggtggcca tcacgcccgc catgcgcgaa ggctcgggca
     4201 tggtcgagtt tcaccggcgc tttcccgacc gctattacga cgtcggtatt gccgagcagc
     4261 atgcggtgac tttcgccgcc ggcctggcct gcgaaggcct gaagccggtg gtggcgatct
     4321 attccacgtt cttgcagcgt gcctacgacc agttgatcca cgacgtggcg atccagaagc
     4381 tgcccatggt gctggcgctc gaccgcgccg ggctggtggg cgccgatggc gccacccacg
     4441 cgggcgcctt cgacatcgcg tacctgcgct gcgtgcccaa cgtcagcgtg gcctgcccgg
     4501 ccgatgagcg cgagtgccgg caattgctca gcaccgcttt tgcgcaagac cacgccgtgg
     4561 cggtgcgcta tccgcgcggc gcgggcgtcg gcgtacagcc gctggcggat ttaagcgaat
     4621 tgcctttcgg caagggtgca gtgcgccgcc agggccagcg catcgccatg ctggccttcg
     4681 gcagcctgct gtatcccgcc ttgcaggcgg ccgagcgctt gggcgccacg gtggtcaaca
     4741 tgcgctgggc caagccgctc gataccgaac tgctgctgca gatcgccagc agtcatgagg
     4801 cgctcgtgac gatcgaggaa ggcgtggtga tgggcggcgc gggcagcgcg gtggccgagg
     4861 cattggcggc ggcccaaatc gccaagccgc tgctgcaact tggcctgccc gatgccttca
     4921 tcgagcacgg cgacccggcg cgcctgctgg cactgcaggg gctggacgca gtgggcatcg
     4981 aagcctccgt gcgaggccgg tttggtgatc tgattgggcc agcagcgccg gtgctcaagg
     5041 tcgtcggcta aaggcacagg ccgccaaggc gacaggtaaa tccctaatcg gcgcgtggcg
     5101 gcggcgggga taaactcaac tgcttgtctt ttgttacacc gtcttcaaca aggagtcaaa
     5161 cctcatggat cgtcgttcac tcatcaagcg caccggtatc gccggtgtgc tggccgccgg
     5221 cgtggcccct gccgtgcacg cgcagccggc cgtgcgctgg cgcctggcct cgagcttccc
     5281 caagtcgctg gacacgatct tcggcagcgc cgagaagatg tcgcagaccg tcaaggcgct
     5341 gtcgggcggc aagttcgagg tgtcggtgca ccccgcgggt gaactgatgc ccgccttcgg
     5401 cgtggtcgac gcgctggaga aggacaccat cgagatggcc cagtccgccg cctactactt
     5461 cgccggcaag gacccgatct tctcaatgag ctgcgcgatt ccgttcggcc tgacgacgct
     5521 gcagatggcg gcgtggaagc tccacggcaa tggtggcaag ctcctgaacg agttcttcgc
     5581 caagtacaac ttccgcacca tgagcgccgg caacaccacc acgcaaatgg gcggctggta
     5641 tcgcaaggaa atcaagtccg tggccgacct gaagggtctg aagatgcgcc tgggcggcgg
     5701 cattttcggc gaggccatgg ccaagctggg cgtggtggcg caaagcctgc cggccggcga
     5761 catctaccag gcgctggaaa aaggcacgct ggatgccgct gaattcgtcg gcccctacga
     5821 cgatgaaaaa ctcggcttcc acaaggtcgc gccctactac tactaccccg gctggtttga
     5881 aggcagcgcc aatctggagt tcttcatcaa caacaagaag tacgaagccc tgtcggccga
     5941 gaacaaggcc atcctgcaag ccgcctgtgc cgtggcggcg caggacatga cggccaagta
     6001 cggcgcccag aacccgcccg cgctcaagcg tctggtgggc ggcggcacca agctcacctt
     6061 gttccccaag gcggtgatgg acgccgcctg gaaggccacg caggaagtgt gtgctgccca
     6121 ttcggccaag tcgcccgagt tcaagaagat ctacgacgac atgcgcaact tccagcgcga
     6181 ccagatcctc tggaaccgct tctccgagta cccgttcaac cagtacatga acacgctcaa
     6241 gatctgacgc gttcgatcac cacggtggcc gtgttgcacg cggccaccga aacaaaaaag
     6301 ccgcgatgca tcgcggcttt tttgtttttc aagaatctga aaatactaca ttttcaatag
     6361 ctgctcgcgc ttgcccagca agcgcgggag cctatttctt tatagattcc tgcagcgatt
     6421 tcagggggtc gtcgctgcca tacccgcccg gggtgggggc ggccgacgac tcttcggcgc
     6481 tggcggccga tgcgggatcg gcaggggcga cggccccgtc ggcctctgac gccgcgccag
     6541 cgggcgcctc ggagccgctg gcatcgttgc cccagaggtt ttcggacgtc gccgcgtcgc
     6601 ctgcgccggg cgcacccagg ctgtcgcgca tctgcgcgcc cacggcgtcc atgtcgacgt
     6661 cgaccttctt gtccaggctg ccggtgacca ggctggggaa ggcgatcagc accgccacca
     6721 tcaccaattg cagcaccagg aaggggatgg cgcccttgta gatttccatc gtagtcaccg
     6781 gctgcatcac cttgcccgtg accttgtcga catacggttt ctcgggggcg accgagcgca
     6841 ggtaaaacag cgcgaagccg aacggcgggt gcatgaacga ggtctgcatg ttcaccgcca
     6901 gcagcacgcc gaaccagatc aggtcgatgc ccagcttgtc ggccaccggc gccagcaggg
     6961 gcacgacgat gaaggacagc tcgaagaaat ccaggaagaa cgccagcacg aagatcagca
     7021 cgttcaccag gatcagaaag cccacctgac cgcccggcag atcgtccagc aggtgctcga
     7081 cccacttgtg cccgtcggcg gcctggaaca ccatgctgaa cacggtggcg ccgaccagga
     7141 tgaacatcac gaacgaggcc agccgcgtgg tgctgcccag cgcctgattg agcagcgcga
     7201 agctcatgcg cccgcgcatc atcgccatga tgagcgcgcc catggcgccc atggcgccgc
     7261 cctcggtcgg cgtggccacg cccaggaaga tggtgcccag caccaggaag atcagcagca
     7321 gcggcggaat cagcacgaag gtcacgcgct cggccaggcg cgagagcagc cccatcctgg
     7381 tgacgcggtt gatgaaggcg atcaaccagg ccacggtgac gccgccgcac atggcgacga
     7441 cgatcttctc gtcggttggc accgattcga ccagcgttcc ctgccaccag gtgtgcacct
     7501 cggccatgtg gcgcgccaga aaaaccgcca ccagcaccga caccacggtc agcaccagca
     7561 gggaggtgta gccgctgctg ccattgggct cgcggaacac gcgcgcttca accggcagcg
     7621 caggcaccat cttgggcttg aagatcgcca gcagcaccac gtacagcacg tacatgccga
     7681 ccagcatgaa agccggcacg aaggcgccct tgtacatgtc gccgacgctg cggcccagtt
     7741 ggtcggccat gatgatcagc accagcgagg gcggaatgat ctgcgccagc gtgcccgagg
     7801 cggcgatcac gccgctggcc aggcgccggt cgtagccgta gcgcatcatg atgggcagcg
     7861 aaatcaggcc catcgagatg accgaggccg ccaccacgcc ggtggtggcc gccagcagcg
     7921 cgccgacgaa gatcaccgcc agcgccaggc cgccgcgcat cgggccgaac acctgcccca
     7981 cggtgtcgag caggtcctcg gccatgccgc tgcgctccag caccaggccc atgaaggtga
     8041 agaagggcac ggccagcagc gtgtcgttgg ccatgatgcc cagcagccgc tgcggcagcc
     8101 aagcgatgat ggaggccgga aagacgttga gctcgatgcc cagcagtccg aagaacaggc
     8161 cgcaagcgcc caggctgaac gcgaccggaa agcccagcag caaaaagcag atcagccccg
     8221 cgaacatgaa cgcggcgtag ttggcgataa agaattccat cttggcttct ccgggcgatc
     8281 agcgtgtggt gtccgcggcc ggcgcggcat cggggctggt ctcgcggcgc agcgcctcgg
     8341 ccagttcttc ctcggccgac ttgtcggtca ggcggcccat ggggtcaggc ccaacgccgc
     8401 gcaggaaggc aacgcgcttg atcagctccg accagccttg cagcagcagc aggccaaagc
     8461 ccagcggcag ggccagccac accggccagc ggaccagccc gcccgcattg ggcgacacct
     8521 cgcccgactg gaatttgctg atcagcatgg gaatgcccag ataaagaacg acgatgcaca
     8581 gcggcgtcag gaagaaggca aagccgatga tgtcgatcca cacctgcgcg cgcttgggca
     8641 ggcggctgtt cagcacgtcg atgcgcacgt gctcgcggtg caacagcgtg tagccggcgg
     8701 cgatcaggaa cgaccaggcg aacaggtacc actgcacctc aagataggcg ttggagctga
     8761 agttgaacgc cttgcgcacg acagcgttga gcgcgctgat gactgtggcg gcaaagataa
     8821 gccagatggc atatttgccc accgtggcat tgagccagtc gatggcccgg gacagtttga
     8881 gaagtgcttg catgaggtct cctgtcttgg ccggctcaga tcggcgcggc ctgtacgttt
     8941 tgaggtgcgc gattgtagta acaggtggcg ctactcggta agcggactat cccgtaagtg
     9001 tcagcgccgc gcaggcatgc ccgcgcggcg cttgggctgt ttgaactgcc accacggcag
     9061 cacgctggcc atcgatcgca cctcgccggc gtgcgaggcc tggtagccgg gcagcgcgtt
     9121 cagctcatgg gcccagcccg cgctggccag cagcgcgcgc agcgccgcca ccggggcttc
     9181 gtccagggcc tctttcaggc acaccaggtg gtaatcctcg gcgatcagcg gcacgaaatc
     9241 caggccgcgc gcgcgcgcgg cggcctcgat gcccaagccc gcatcggcgc cgccggccgc
     9301 caccgcctgc gcgacggcgg cgtgcgaggg ctcgacgtcg tggtagccgc gcacgtcggc
     9361 ggccgccaga tcctcggcgg ccagcaactc gtccagcagc acgcgggtgc cggtgcccag
     9421 cgcccggttg acgaagcgca cgccggcctt gttgaggtct gcgagccctt gaatgcggtg
     9481 cgggttgccc ggccgcacca tcaggccttg cgtgcgcgtg gcaaagccga tcagcttgtg
     9541 caggccgggt tgcagcagcg gcttgtaggt gcgcgcggcc agcgaatcgg ccgctggcgt
     9601 gcgcagcgtg tgaaagccgc ccatgatgca gcgcccttcg ttgagcgccg caatggcgtc
     9661 cacgctgccg gtgaagcgga tgtcgaggtg caggcgcggc gtgccgggca ggctggccgc
     9721 gtgctggcgc agggcgacca gcgcgtcgtc gtggctggcg tacagcgtca gcacgtgcgc
     9781 ggcgtcgtca aaggcgacgg caaaggtgcg ctccagttcg gcgcgcagcg cctcgatctg
     9841 cggcgccagc cgggcctgcg cctggcgctc tgaccacagc agcttgtcgc cgaactcgga
     9901 caggcgggcg cgccggcctt tttcccagtg caagagcggc tgccccagct cctgctccca
     9961 gcggcgcagc tcgccccaca cgtggcggta cgacagaccg agcgcgctgg ccgcgctgga
    10021 gatcgagccg ctgctgcgca ccgcctgcag cagatcgagc agcgggttgc gaatcagtgg
    10081 gccgccgccg tcggtgccga gcgagtagga caggccgatt ttctgcattg ccccgagtgt
    10141 gcccgacgcg ctggcgcggc tggcgcgcag ccgcgcgccg acgacccaac ctatgcactc
    10201 aattgcatag ctcgccatca ccgattcacg ggcgcggcga cgggggttag catcaacgcc
    10261 gccttgaaca cctttgtcga cagcgggcgc accgcgcttg aactcatcac ttcgctcgat
    10321 ccagccctgt ggaccgtcgt cgcgcgctcg ctggccgtca gcgccacggc gtgcgccctg
    10381 ggctatggcg tgggcgtggt ggcgggcgcg tggctgggcg tgacgcgctt tcgcggccgc
    10441 aacgtggtgc tgacgctgct caatacgctg ctggcgctgc cctcggtggt ggtcggcctg
    10501 ctggtctacc tgctgctgtc gcgcagcggg ccgctgggct ttctgggctg gatgttcagc
    10561 ttcaaggcca tggtggtggc gcaggtggtg ctggtgttgc cggtggtgac cgcgctgacg
    10621 cgccaggcgg ttgaagacgc cgatctgcag cacggcgagc aactggcgtc gctgggcgcc
    10681 gggcgcttca tgcgtgggct gctgctggcc tgggacgagc gctacgcgct gctcacagtg
    10741 ctgatggcgg ccttcggccg cgcgatttcc gaggtcggcg cggtgatgat cgtcggcggc
    10801 aacatcgacg gctttacccg cgtgatgacc accgccatcg cgctggaaac cagcaagggc
    10861 gatctgccgc tggcgctggg cct
//
LOCUS       DAOMVO010000047        10539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1516_length_10539_cov_15.228443, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000047 DAOMVO010000000
VERSION     DAOMVO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_10539_cov_15.228443"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKV30_03930"
     CDS             <1..522
                     /locus_tag="PKV30_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRL29403.1"
                     /translation="AADVLARLHQPRPAPAALQPLHERFGALFRRVTTPHDAAYALAA
                     DIAHELLDALHTPVALHGDLHHENVVHGARGWLAIDPKGVLGDAAFDAANWFYNPWGQ
                     DALHRQAERIAALAQVLAAPLGCTSAHVLRHAVAYGGLSVAWFAEDGDVANERRTLTT
                     LQAVHAAWRAGGF"
     gene            541..912
                     /gene="ligA"
                     /locus_tag="PKV30_03935"
                     /pseudo
     CDS             541..912
                     /gene="ligA"
                     /locus_tag="PKV30_03935"
                     /inference="COORDINATES: protein motif:HMM:NF009917.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
     gene            1006..1905
                     /locus_tag="PKV30_03940"
     CDS             1006..1905
                     /locus_tag="PKV30_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HRL29404.1"
                     /translation="MEKAQAATENAAQGQARITASVYSSHVPAIGAAIDLGKTEEPYW
                     VPLFKGYEFSKQWMKEHTPDVIFLVYNDHATSFDLGVIPTFAIGTAQEFQPADEGYGP
                     RPVPVVKGHPALASHIAQSVIQQDFDLTIVNDLKVDHGLTVPMNLMFGSPAEWPCQVI
                     PFHVNVVQYPVPSGARCFALGQAIRRAIESYDEPLKVQIWGTGGMSHQLQGPRAGLIN
                     ADWDNRFLDRLIADPKDLSTMPHIDYVREAGSEGIELVMWLIARGAMADVAGGLAPTV
                     RHRFFHVPASNTAVGHLIMENQA"
     gene            1950..2906
                     /locus_tag="PKV30_03945"
     CDS             1950..2906
                     /locus_tag="PKV30_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRL29405.1"
                     /translation="MKNINVALAGAGAFGIKHLDGIQNIDGIQVVSLISRDLEKTQEV
                     ADKYGIAHVTTDLAESLARPEVDAVILCTPTQMHAEQSIACLQAGKHVQVEIPLCDNL
                     ADGLRVAEVQKKTGLVAMVGHTRRFNPSHQYVHQKIKAGEFNIQQMDVQTYFFRRTNT
                     NALGQARSWTDHLLWHHAAHTVDLFAYQAGAPIVKANAIQGPIHPELGIAMDMSIQLK
                     AANGAICTLSLSFNNDGPLGTFFRYIGDTATYIARYDDLVNGKEEKIDVSQVDVSMNG
                     IELQDREFFAAIREGREPNASVAQVLPCYQVLHDLEQQLNAA"
     gene            3090..3560
                     /locus_tag="PKV30_03950"
     CDS             3090..3560
                     /locus_tag="PKV30_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRL29406.1"
                     /translation="MKRWIKRSLVGLAGLGIVLGSLTACGHRMHHGGWHGDATPEQQA
                     EWRGKMVERVSAKLDLNAEQKAKLTVLSEKMQAQRQALRGSADPRAEMQSLVAGAQFD
                     RAKAQALVQQKTDALRSGSPEVIAALGDFYDSLNPAQQQKVREFMSHRGGWRRG"
     gene            3661..4347
                     /locus_tag="PKV30_03955"
     CDS             3661..4347
                     /locus_tag="PKV30_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL29407.1"
                     /translation="MTHILLIDDDEHLGPPLAHYFQRFGLQLAQALTPADGLARLRAG
                     GVDIAILDVMLPEMDGFALCRIIRAESRVPILMLTARGEVMDRVVGLELGADDYMPKP
                     FEPRELVARVQNVLRRRESAPEPLAADPALRFDGLLVNPATRSVQRQGAGVELTSTEF
                     DLLHLLARSPGKVFSRDDILAALRGHEVELHTRAVDILVSRLRKKLEPLDAVKTLRNA
                     GYTLALQRQR"
     gene            4347..5717
                     /locus_tag="PKV30_03960"
     CDS             4347..5717
                     /locus_tag="PKV30_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL29408.1"
                     /translation="MSEAPAASPWRRLRHSLRLRLVLLFLLLALAMSVAFIGGVGKAL
                     SAGWRGATEPLFVDYADRLVDELGTPPSIARAQALAERLPLAVRISGPAVNWVSGPWP
                     RHGPPWARAEEEGGAGASAMRARNTVSRQTSDGHRVEFSLKTWRADDDKKNFVWFTLA
                     ALLALTALAYGVVHRMLRPIRDIGEGVRRFGAGQFEPQIRVRQGAHADELSDLAHSVN
                     RMAADIHQMLDAKRDLLLAISHELRTPLTRARLNAELLAEDADTAPTRAALLRDLAQM
                     RDLITDLLESERLASRHAALQREATDLPALAHALIGDMAALRAAAADVPVHVAPSLTA
                     PLWLDAGRTRLLLRNLLDNALRHTPTGAAPPELHLGVADNGHVLIEVRDHGPGVPPEQ
                     LARLTEAFYRPDDARQRSTGGVGLGLYLARMVAEAHGGRLTVRNAQPGLAVRAEWPAA
                     AVPAES"
     gene            complement(5799..8216)
                     /locus_tag="PKV30_03965"
     CDS             complement(5799..8216)
                     /locus_tag="PKV30_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRL29409.1"
                     /translation="MTEPSKPMPPTTAPAEDAAPVVAPRIAGEVASHTAEALSRPPLM
                     NGLVSVAVIVAALYFGRDLLMPLALAILVGFVLDPMVSWLKRRGVPRALAVAIVVMAT
                     VALLLASAFFIVGQLRQISNDLPVYETNITRKLRSFGQSLRQPGVLDRYSRVVTRVER
                     ELDAAQRQAEGPKPRAEQPTRVEIVGQAVTPWQRVMAWGESFATPLALVGIVFVFVVL
                     ILLDKGDLRDKVVRLLGSNLHRTTDALGDAAKRVSKYLTMQLLVNATYGIPMALGLLF
                     IGVPGALVWGLLAAVLRFVPYVGPMIGAIFPLTLAFGVDPGWNMVLWTVALIATLELI
                     SNNLVEPWLYGTSTGMSTLSLILAAMFWTALWGPIGLVLSTPMTVVLLVLGHYLPQLQ
                     FLEVLLGSERALDEPTRLHQRLLAGDVEEAVDMAEQHAEQTSPQHFYDHVGLGALRLA
                     ATAQDTVATAEHRHRVVSGMERVIDELRDSYPPPDELPLRVACIGGRWAMDSLAADMA
                     AHVLTLNGVGARVLQLGVMSSDYFARLDLRGVEVICLSFFSPDPTTLAKYFVRRLKRR
                     WPDLQVVLAAWSYEPSAQVAHPMEETGADAFVTTLDELVVQAQNRLASAAGTPYVPPE
                     VPENETERLQALQDSGALNPALRGRFDALAKRAADVFDCQGAHIALVNEAWQLTHGDA
                     GAAGRPDEGAPEEGAPREQSMSGHVVALGEPLVVPDVQRDARFAANPLLAERGIRFFA
                     GAPLRTADGFVLGALGVFDDKPRTLSPRDVMLLGHMADEVMQAARQQAAKAPPDPAPD
                     ATSPAAG"
     gene            8452..9204
                     /locus_tag="PKV30_03970"
     CDS             8452..9204
                     /locus_tag="PKV30_03970"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRL29410.1"
                     /translation="MLDHPDRSLGLSGASNFRDLGGYAGTGGRPVRWRRLFRSDHLAA
                     LTPEDARTFQQLGVTRTFDFRGTGERAAVPYDLPGVTQHALPIEPTVVQRMKDLLDAG
                     ETVTPAHTVELMQHTYRAFVHDNATRFRALFSHLLESDAPLVFHCTAGKDRTGFAAAL
                     ILLALGVPRDVVMQDYLLTNDFYRMPALDESRAPREVLNVLWRVQQEFLDAALHAVEH
                     DHGGIDAYLSDALSVGRAERQRLAELYLAPAR"
     gene            complement(9275..>10539)
                     /locus_tag="PKV30_03975"
     CDS             complement(9275..>10539)
                     /locus_tag="PKV30_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRL29411.1"
                     /translation="TLREARNREEFLTVAQQILGFPIDVIAGREEARLIYQGVAHLLP
                     QSDERRLVLDIGGRSTELIIGSGYAPQVMESYRVGSVAWSMRYFPDNVLSARNFERAE
                     IAAKAVLDDAATTHGRKQWDQAYGCSGTVGAIADTLLLAERSPARYQITREGLDWLRD
                     KLIKAGRFDAIKLEGIKEERKAVIAGGLSVLRALFDLLQIDSLQYAYGALRHGALYDM
                     LDREQEQTDVRATAVAALAAKFSADAAQGERVSRAALALFDQLAPATLKKKQREELKR
                     EIDWAARLHEIGSRISHADAHKHGAYILGHADVAGFAMHELHDLSQLVLGHRGKLRKL
                     EARFGDPLFMRQLLALRLAVILCHARQDPETQHVSISADAEPAQGFTLSVGNGWAERW
                     PQSAHLLREEVIAWQKTPWGLRLRASGR"
BASE COUNT         1733 a         3653 c         3572 g         1581 t
ORIGIN      
        1 gccgccgacg tgctggcgcg tttgcaccag ccgcgaccag caccagcggc cctgcagccc
       61 ttgcacgagc gtttcggcgc gctgttcagg cgcgtcacca cgccgcacga tgcggcctat
      121 gcgctggccg ccgacattgc gcacgaattg ctggatgcgc tgcacacgcc cgtcgcgctg
      181 catggcgacc tgcaccacga aaacgtcgtg cacggcgcgc gcggctggct ggccatcgac
      241 cccaaaggcg tgctgggcga cgcggccttc gatgccgcga actggttcta caacccgtgg
      301 ggccaagacg ccctgcaccg ccaagccgag cgcatcgccg cgctggcgca ggtgctggcc
      361 gcgccgctcg gctgcacgtc cgcgcacgtg ctgcgccacg ccgtggccta cggcggcctg
      421 tcggtcgcct ggtttgccga agacggcgac gtggccaacg aacgccgcac gctcaccacc
      481 ctgcaagccg tgcacgccgc gtggcgtgcc ggcggttttt gacattggaa ggagacaaac
      541 atggccctcg acaaacccta tctcgacgtg cccggcacga cgatcttcga cgccgagcag
      601 agccgcaagg gctactggct caaccagttc tgcatgagcc tgatgaaggc cgagaaccgc
      661 gcgcgcttca agcagggcga agacgcctac ctggccgaat ggccgatgac cgacgagcag
      721 aaagcggcgg tgcgtgcgcg cgatctgaac tggatgatga gcaccggcgg caacatctac
      781 tttctgtcca agctgggcgc caccgatggc ctgagttttc agcagatggc cggcagcatg
      841 accggcatga ccgagcagca gtaccgcgac atgatgatct cgggcgggcg cagcgccgaa
      901 ggcaaccgca tcgtcggcga acagggcaac gcgcaaggtg cgatgtcgca gtccgaggcc
      961 tggaaagcct gggaagaaga agcacaaaaa gagctggcag cgcgggtgga gaaagcgcag
     1021 gcagctaccg aaaatgcagc gcaaggacag gcccgcatca ccgcctcggt ctacagctcg
     1081 cacgtgccgg cgattggcgc tgccatcgat ttgggcaaga ccgaagagcc gtattgggtg
     1141 ccgctgttca agggctatga gttcagcaag cagtggatga aggagcacac gccggacgtg
     1201 atctttctcg tctacaacga ccacgccacc agctttgacc tgggtgtgat tcccactttt
     1261 gccatcggca cggcgcaaga atttcagccc gccgacgaag gctatggccc gcgtccggtg
     1321 ccggtggtca aaggtcaccc cgcgctggcc tcgcacatcg cgcaatcggt gatccagcag
     1381 gacttcgacc tcaccatcgt gaacgacttg aaggtcgacc acggcctgac ggtgccgatg
     1441 aacctgatgt tcggcagccc cgccgaatgg ccgtgccagg tgattccctt ccacgtcaac
     1501 gtggtgcagt acccggtgcc ttcgggtgcg cgctgctttg cgctggggca ggcgattcgc
     1561 cgcgccatcg aaagctacga cgagccgctc aaggtgcaga tctggggcac cggcggcatg
     1621 agccaccagc tgcaaggccc gcgcgccggc ctcatcaacg ccgattggga caaccgcttt
     1681 ctcgaccgcc tgatcgccga cccgaaggac ttgtcgacca tgccgcacat cgactacgtg
     1741 cgcgaagccg gcagcgaagg catcgagctg gtcatgtggc tgatcgcccg cggcgccatg
     1801 gccgatgtgg ccggcgggct ggcgccgacg gtgcggcacc gcttcttcca cgtgccggct
     1861 tcgaacaccg ccgtgggtca tctgatcatg gaaaatcagg cctgagcgct tgctggacaa
     1921 gcgcgagcag ctatcaatca aggagtagca tgaaaaacat caacgtcgcg ctggccggtg
     1981 ccggtgcctt cggcatcaag cacctggacg gcatccagaa catcgacggc atccaggtgg
     2041 tgtcgctcat cagccgcgat ctggaaaaaa cgcaggaagt ggccgacaag tacggcatcg
     2101 cccacgtcac gaccgatctg gccgaaagcc tggcgcggcc cgaggttgac gccgtcatcc
     2161 tctgcacgcc gacgcagatg cacgcggagc aatcgattgc ctgcctgcag gccggcaagc
     2221 acgtgcaggt cgagattccg ctgtgcgaca acctggccga tggcctgcgg gtggccgagg
     2281 tgcagaagaa gaccggcctg gtggccatgg tcggtcacac gcgccgcttc aaccccagcc
     2341 accaatacgt gcaccagaag atcaaggcgg gcgaattcaa catccagcaa atggatgtgc
     2401 agacctattt ctttcgccgc accaacacca acgcgctggg ccaggcccgc agctggaccg
     2461 accacctgct gtggcaccac gcggcgcaca cggtcgatct gtttgcctac caggccggcg
     2521 cgcccatcgt gaaggccaac gccattcagg gcccgatcca tcccgagctg ggcattgcga
     2581 tggacatgag catccagctg aaagcagcca acggcgccat ctgcacgctc agcttgagct
     2641 tcaacaacga cggcccgctg ggcaccttct tccgctacat cggcgacacc gccacctaca
     2701 tcgcccgcta cgacgacttg gtgaacggca aggaagagaa gatcgacgtg agccaggtcg
     2761 atgtgagcat gaacggcatt gagctgcaag accgcgagtt cttcgccgcc atccgcgaag
     2821 gccgcgagcc caacgccagc gtggcccagg tgctgccgtg ctaccaggtg ctgcacgatc
     2881 tcgagcagca gctgaatgct gcgtaattca tagctgctcg cgcttttcag acaagcgcca
     2941 gcgcctgatt tctttcaagg ctgcatgttg ccgcccttct gcgcggtgac ataaaacgaa
     3001 acaaagcgat acccgggtga gacccggcgg cggggcttgc ccggcatcat ggcgatcatg
     3061 ttcaacccgc cttgagaagg ggcaccgtca tgaaacgctg gatcaaacgc agtcttgtgg
     3121 gcctggctgg cctgggcatc gtgctgggca gcctgacggc ctgtggccac cgcatgcacc
     3181 acggcggctg gcacggcgac gccacgcccg agcagcaggc cgaatggcgc ggcaagatgg
     3241 tcgagcgcgt ttcggccaag cttgacctga acgccgagca gaaagccaag ctcaccgtgc
     3301 tgtccgagaa gatgcaggcg cagcgccagg ccttgcgcgg cagcgccgat ccgcgcgccg
     3361 agatgcagtc gctggtcgcc ggtgcccagt ttgaccgcgc caaggcgcag gccctggtgc
     3421 agcaaaagac cgatgccttg cgcagcggca gccccgaagt gatcgccgcc ttgggcgact
     3481 tctacgacag cctcaatccg gcgcagcagc aaaaggtgcg cgaattcatg agccaccgcg
     3541 gcggctggcg ccggggctga cgtgacggcc gtcgtgctga gcgcgccgcg cgccctgcat
     3601 cgcctgggca gcgcgccggc cgacgcgggg cgaggggcgg cggacgcgac acaatcgggc
     3661 atgacccaca tcctgctgat cgacgacgac gagcacctgg ggccgccgct ggcgcattat
     3721 ttccagcgct ttggcctgca gctggcgcag gcgctgacgc cggccgacgg gctggcgcgg
     3781 ctgcgcgccg gcggcgtcga catcgccatc ctcgacgtca tgctgcccga gatggacggc
     3841 tttgcgctgt gccgcatcat ccgcgccgaa agccgcgtgc cgatcctgat gctgaccgcg
     3901 cgcggcgagg tgatggaccg cgtggtcggg ctggagctgg gcgccgacga ctacatgccc
     3961 aagccgttcg agccgcgcga gctggtggcg cgcgtgcaga acgtgctgcg ccggcgcgaa
     4021 tcggcgcccg agccgctcgc ggccgatccg gcgctgcgct ttgacggcct gctggtcaac
     4081 cccgccacgc gcagcgtgca gcggcagggc gcgggggtcg agctgaccag caccgaattc
     4141 gatctgctgc acctgctggc gcgctcgccc ggcaaggtgt tcagccgcga cgacatcctg
     4201 gccgcgctgc gcgggcacga ggtcgagctg cacacgcgcg cggtcgacat cctggtcagc
     4261 cgcctgcgca agaagcttga gccgctggat gccgtgaaga cgctgcgcaa cgccggctac
     4321 acgctggccc tgcagcggca gcgctgatga gcgaagcgcc cgccgccagc ccctggcgcc
     4381 gcctgcggca ttcgctgcgc ttgcggctgg tgctgctgtt tctgctgctg gcgctggcga
     4441 tgagcgtggc gttcatcggc ggtgtcggca aggcgctgtc ggccggctgg cgcggcgcca
     4501 ccgagccgct gttcgtcgac tacgccgacc gcctggtgga cgagctgggc acgccgccca
     4561 gcatcgcgcg ggcgcaggcg ctggccgaac gcctgccgct ggcggtgcgc atttcggggc
     4621 cggcggtcaa ctgggtgtcc ggcccctggc cgcgccacgg gccgccctgg gcgcgcgccg
     4681 aagaagaagg cggggcgggc gccagcgcca tgcgggcgcg caacaccgtc agccggcaga
     4741 cgtccgatgg ccaccgggtc gagttctcgc tgaagacctg gcgcgccgac gacgacaaga
     4801 aaaatttcgt ctggttcacg ctggcggcgc tgctggcgct gacggcgctg gcctacggcg
     4861 tggtgcaccg catgctgcgg ccgatccgcg acatcggcga gggcgtgcgc cgctttggcg
     4921 ccgggcagtt cgagccgcag atccgggtgc ggcagggcgc ccatgcggac gagctgagcg
     4981 acctggcgca cagcgtgaac cgcatggccg ccgacatcca ccagatgctg gacgccaagc
     5041 gcgacctgct gctggccatc agccacgagt tgcgcacgcc gctgacgcga gctcggctca
     5101 acgccgaact gctggccgag gacgccgaca ccgcgcccac gcgcgccgcg ctgctgcgcg
     5161 acctggcgca gatgcgcgac ctgatcaccg acctgctgga aagcgagcgc ctggccagcc
     5221 gccacgccgc gcttcagcgc gaggcgaccg atctgcccgc gctggcgcat gcgctgatcg
     5281 gcgacatggc cgccctgcgc gccgccgcgg ccgatgtgcc ggtgcacgtg gcgcccagtc
     5341 tgacggcacc gctttggctc gacgccggcc gcacgcgcct gctgctgcgc aacctgctcg
     5401 acaacgcgct gcgccacacc ccgaccggcg ccgcgccgcc cgaactccac cttggcgtgg
     5461 ccgacaacgg ccacgtgctg atcgaggtgc gcgaccacgg cccgggcgtg ccgccagagc
     5521 aattggcgcg cctgaccgaa gccttttacc gccccgacga tgcgcgccag cgcagcaccg
     5581 gcggggtcgg cctgggcctg tacctggccc gcatggtggc cgaggcgcac ggcggccgcc
     5641 tgaccgtacg caatgcgcag ccgggcctgg cggtgcgcgc cgaatggccg gcggcggcgg
     5701 tgcctgcgga atcctgattt ctatcaaatc gatagctgct tgcgcttatc cgtcaagcgc
     5761 tggcggcctg tttgactccg aatttcggac cgatgcgctc agccggcggc tggcgaggtc
     5821 gcgtccggcg ccggatcggg cggcgccttg gccgcctgct ggcgcgccgc ctgcatcacc
     5881 tcgtcggcca tgtggccgag cagcatgaca tcgcgcggcg aaagcgtgcg cggcttatcg
     5941 tcgaacacgc ccagcgcgcc cagcacgaag ccgtccgccg tgcgcagcgg cgcgccggca
     6001 aagaagcgga tgccgcgctc ggccagcagc gggttggcgg caaaacgcgc gtcgcgctgc
     6061 acgtcgggca ccaccagcgg ttcgcccagc gccaccacgt ggccgctcat cgattgttcg
     6121 cgcggcgcgc cttcttcagg cgcgccttcg tcgggccggc cggccgcgcc ggcatcgcca
     6181 tgcgtcagct gccacgcctc gttcaccagc gcgatgtggg cgccctggca gtcgaacaca
     6241 tcggcggcgc gcttggccag cgcgtcgaag cgcccgcgca gcgcggggtt cagcgcgccg
     6301 ctgtcctgca aggcctgcag gcgttcggtc tcgttctcgg gcacttccgg cggcacgtag
     6361 ggcgtgccgg cggcgctggc cagccggttt tgcgcctgca ccaccaactc gtccagcgtg
     6421 gtgacaaacg catcggcgcc agtctcttcc atcgggtgcg cgacctgtgc gctcggctcg
     6481 tagctccacg ccgccagcac cacctgcaag tcgggccagc ggcgcttgag gcgacgcacg
     6541 aaatacttgg ccagcgtggt cgggtcgggc gagaaaaagc tgaggcaaat cacctcgacg
     6601 ccgcgcagat cgagccgcgc gaaatagtcc gacgacatca cgcccaactg cagcacgcgg
     6661 gcgccaacgc cgttcagcgt cagcacgtgg gcggccatgt cggcggccag cgaatccatg
     6721 gcccagcgcc cgccgatgca ggccacgcgc agcggcagtt catcgggcgg cggatagctg
     6781 tcgcgcaact cgtcgatcac gcgctccatg ccgctcacca cgcggtggcg gtgctcggcg
     6841 gtggccacgg tgtcctgcgc ggtggccgcc aggcgcagcg cgcccaggcc gacatggtcg
     6901 tagaagtgct gcggcgaggt ctgctcggca tgctgctcgg ccatgtcgac ggcctcttcg
     6961 acatcgcccg ccagcaggcg ctggtgcagg cgcgtgggtt cgtccagggc gcgttcgctg
     7021 cccagcagca cctccagaaa ctgcaactgc ggcaggtagt ggcccagcac cagcagcacc
     7081 accgtcatcg gcgtcgacag caccagcccg atcggccccc acagcgcggt ccagaacatg
     7141 gccgccagga tcagcgacag cgtcgacatg ccggtgctgg tgccgtacag ccagggctcg
     7201 acgaggttgt tgctgatcag ctccagcgtg gcgatcagcg ccaccgtcca cagcaccatg
     7261 ttccagccgg ggtcgacacc aaacgccaat gtgagcggaa aaatggcgcc aatcatcggc
     7321 cccacgtacg gcacgaagcg cagcaccgcc gccagcaagc cccacaccag cgcacccggc
     7381 acgccgatga acagcaggcc cagtgccatc ggtatgccgt aggtggcgtt gacgagcaac
     7441 tgcatggtca ggtacttgct gacccgcttg gcggcatcgc ccagcgcgtc ggtggtgcgg
     7501 tgcaggttgc tgcccagcag gcgcaccacc ttgtcgcgca gatcgccctt gtcgagcagg
     7561 atcagcacca cgaacacgaa cacgatgccg accagcgcca gcggcgtggc aaacgattcg
     7621 ccccacgcca tcacgcgctg ccacggcgtg acggcctggc cgacgatctc gacgcgggtc
     7681 ggctgctcgg cgcgcggctt cgggccttcg gcctggcgct gcgcggcgtc cagttcgcgc
     7741 tcgacacgcg tgaccacgcg cgaatagcgg tccagcacgc ccggctggcg cagggactgg
     7801 ccaaagctgc gcagcttgcg cgtgatgttg gtctcgtaga ccggcaggtc gttgctgatc
     7861 tgccgcaact ggccgacgat gaagaaggcg ctggccagca gcaaggccac cgtcgccatg
     7921 acgacgatgg ccaccgccag cgcgcgcggc acgccgcggc gcttgagcca cgacaccatc
     7981 gggtccagca cgaagccgac caggatcgcc agcgccagcg gcatcagcaa atcgcgccca
     8041 aagtagagcg ccgccacgat gacggccacg ctgaccaggc cgttcatcag cggcgggcgc
     8101 gacagcgcct cggccgtatg cgacgccact tcgccagcga tgcgcggcgc cacgaccggc
     8161 gccgcgtctt cggccggcgc ggtcgttgga ggcatgggct tggatggttc ggtcacgggc
     8221 gcactccctg tgggtgctcg cgctgcgccg ccgcgcacgc gcaccacttc cgccctgcgt
     8281 ggcggggcgt gtggggattg tgggcagtgg gtgggcgtcg gcgtgtagga cgagcgcgcg
     8341 gcaagggcgg cggctgtcgg gctgcggctg gggcgccaca aacgggctgt catgcgcatc
     8401 gccgaaaatg gcgcattgct tttctcgccg gcacgcgccg gccgcacgct catgctcgac
     8461 catcccgatc gctcacttgg cctgtccggc gccagcaact tccgcgacct gggcggctat
     8521 gcgggcacgg gcggccggcc ggtgcgctgg cgccggctgt ttcgctccga ccatctggcg
     8581 gcgttgacgc ccgaggatgc gcggacgttt cagcagctcg gcgtcacgcg cacgttcgac
     8641 tttcgcggca ccggcgagcg cgccgccgtg ccctacgacc tgccgggtgt gacgcagcat
     8701 gcgctgccca tcgagccgac cgtggtgcag cgcatgaagg atttgttgga cgccggcgaa
     8761 accgtgacgc ccgcgcacac ggtggagctg atgcagcata cctaccgcgc cttcgtgcac
     8821 gacaacgcga cgcgcttcag ggcgctgttt tcgcacctgc tggaaagcga cgcgccgctg
     8881 gtgtttcact gcaccgccgg caaggaccgc actggctttg cggcggcgct gatcctgctg
     8941 gcgctgggcg tgccgcgcga cgtggtgatg caggactacc tgctgaccaa cgacttctac
     9001 cgcatgccgg cgctggatga aagccgcgcg ccgcgcgagg tgctgaatgt gctgtggcgc
     9061 gtgcagcagg aatttctgga cgccgcgttg cacgccgtcg agcacgatca cggcggcatc
     9121 gatgcctacc tgagcgacgc cctgagcgtg ggcagggcag agcgccagcg gttggccgag
     9181 ttgtacctcg cgcctgccag gtaactacaa aattgatagc tgctcgcgct tgtccatcaa
     9241 gcgctggcgg cctgttttgc tttgaatcgc ggggtcagcg accgctggcg cgcagccgca
     9301 ggccccaggg cgttttctgc cacgcaatga cttcctcgcg cagcaggtgc gccgattgcg
     9361 gccaacgctc ggcccagccg ttgccgacgc tgagcgtgaa gccctgcgcc ggttcggcat
     9421 cggcgctgat gctgacgtgt tgcgtttccg ggtcttgccg cgcgtggcac aggatgacgg
     9481 ccaggcgcag cgccagcaac tggcgcatga acagcgggtc gccaaagcgc gcctccagct
     9541 tgcgcagctt gccgcggtgg cccagcacca gctggctcaa atcgtgcagc tcgtgcatgg
     9601 cgaagccggc gacgtcggcg tggcccagga tgtaggcgcc gtgcttgtgc gcgtcggcgt
     9661 gcgagatgcg cgagccgatt tcatgcaggc gcgcggccca gtcgatctcg cgcttcaact
     9721 cctcgcgctg cttttttttc agcgtggcgg gcgccagctg atcgaacagc gccagcgccg
     9781 cgcgcgacac gcgttcaccc tgcgcggcgt cggcactgaa cttggccgcc agcgcggcga
     9841 cggcggtcgc gcgcacgtcg gtctgctcct gctcgcggtc gagcatgtcg tacagcgcgc
     9901 cgtggcgcag cgcaccgtag gcgtattgca gcgaatcgat ctgcagcaaa tcgaacagcg
     9961 cgcgcaacac cgacaggccg ccggcaatca ccgccttgcg ctcttccttg atgccctcga
    10021 gcttgatggc gtcgaagcgc ccggccttga tcagcttgtc gcgcagccag tcgaggcctt
    10081 cgcgcgtgat ctggtagcgc gccggcgagc gctcggccag caacagcgtg tcggcaatcg
    10141 cgccgacggt gcccgagcac ccgtacgcct ggtcccactg cttgcgtccg tgcgtggtgg
    10201 cggcgtcgtc cagcaccgcc ttggcggcga tttcggcgcg ctcgaagttc cgggccgaca
    10261 gcacgttgtc gggaaagtag cgcatcgacc acgccacgct gcccacgcgg tacgactcca
    10321 tcacctgcgg cgcgtagccg ctgccgatga tgagctcggt cgagcgcccg ccgatgtcga
    10381 gcaccaggcg ccgctcgtcg gactgcggca gcaggtgggc cacgccctgg tagatcaggc
    10441 gcgcttcttc gcggccggcg atgacgtcga tcggaaagcc caggatctgc tgtgcgacgg
    10501 tgagaaattc ctcgcggttg cgcgcttcgc gcagcgttt
//
LOCUS       DAOMVO010000048        10527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1519_length_10527_cov_13.590623, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000048 DAOMVO010000000
VERSION     DAOMVO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_10527_cov_13.590623"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..742
                     /locus_tag="PKV30_03980"
     CDS             101..742
                     /locus_tag="PKV30_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29412.1"
                     /translation="MEPALTPLLVLAALATALAFKPWRLLANTALLTPLLAALVITPW
                     LWALPWLHHMPIRLQLSGACLIGLALGWPLAVPALCAVALITGLIAPISLAEQIDMAL
                     WLGIVPATLSFGLGMALRRWVSKNLFVYILGRAFLGTALCMFASGALAHWTGHALAAT
                     VEPELALVARWLIAWGDAFVTGMMAAICVAFRPQWLATWSDRLYVAPPAPPAA"
     gene            775..1470
                     /locus_tag="PKV30_03985"
     CDS             775..1470
                     /locus_tag="PKV30_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRL29413.1"
                     /translation="MTARRFHPLRPFLTGMLALLPLAATLVLLVWATRLAAEWLGPSS
                     LLGSLLTRIGVGFGPGAALPSYLIGLLVVLALVYGFGLLVETGLQRGLDSVIQAMMRR
                     IPVVRTVYDIAHRLVGLFSKPKDEGVKSMRPVWLHFGGPPADGQPADSTVVLGLLTTH
                     EPVLLGGQPFHGVLVPTAPVPVGGGLLFVPAHWVLPADLGIEAVTSIYVSMGVTANQY
                     LGAQAAPPPPDRA"
     gene            1559..1702
                     /locus_tag="PKV30_03990"
     CDS             1559..1702
                     /locus_tag="PKV30_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HRL29414.1"
                     /translation="MIVKLWQDLFGTDYGLMSISGLLFIVFMAFWFVRFFLRKMAEPP
                     PKI"
     gene            complement(1762..3453)
                     /locus_tag="PKV30_03995"
     CDS             complement(1762..3453)
                     /locus_tag="PKV30_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRL29415.1"
                     /translation="MRPHHKFWPARLPHAITPPATSLWQNLAVSAMRYPDKAAIVFFG
                     RVMSWAQLKHDAERLAAQLQALGVSKGDRVVLNMQNCPQLIVAHFAILRADAVVVPVN
                     PMNRAEELKHYITDPDAKVAITTADLAGEMAAASDALPPEQRLQHLIVTQFTDGFDGN
                     IEGDDAPPAAWRDWLLTHHALPALAGGQVHAWQDALAWGGPLADTTAGPADLAILPYT
                     SGTTGLPKGCMHTHASVMHNAVACAMWGGGTSETIGLVVVPMFHITGLVAIMQAAVYA
                     GSTMVVMPRWDRELAGRLISRWRVTAWSNIPTMVIDLLASPNFASFDLSSLKTIGGGG
                     AAMPQAVAQRLLDVYGLAYCEGYGLTETAAPSHQNPVDHPKQQCLGIPFMSTDARVVD
                     PETLQEVPQGEQGEIVIHGPEVFQGYWKRPDATEAAFFELEGKRFFRSGDLGRVDDDG
                     YFFITDRLKRMINASGYKVWPAEVEALMFRHPAIQEACIIATKDAYRGESVKAVVVLR
                     PSHQGQVSEQDIIDWCRDNMAVYKVPRVVQFADALPKSGSGKVMWRALQEAESVG"
     gene            3586..4572
                     /locus_tag="PKV30_04000"
     CDS             3586..4572
                     /locus_tag="PKV30_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRL29416.1"
                     /translation="MHAITLARQLLVATLLGAATLAHAFDLQGHRGARGLAPENTLAA
                     FDRALAEGVNTLELDIILSADGVPMIVHDPFLPPDMTRDAQGQWLPTPTPLIRTLTLA
                     ELQSYDLGRVRPDSRTARDFPQQRPQDGERMPTLAQLFERVKALGAADVRFNIEIKTN
                     PDKPQDTVAPDQFVSAILAVVRAADMQQRVLLQSFDWRPLKLAQQQAPDIPTAYLTVE
                     TRNANNAAAPAWTGGLKRTDYPSIPHMVQAAGGKLWTPNFASLNQAALRQAQQLGLKV
                     IPWTVNEPADMARLIDWGVDGLITDYPDRARAVMRDKGMALPAAIQGAKTIN"
     gene            complement(5129..5467)
                     /locus_tag="PKV30_04005"
     CDS             complement(5129..5467)
                     /locus_tag="PKV30_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRL29417.1"
                     /translation="MKQITAVIKPFKLEEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVSEGDVERCVDAIVKAARTGKIGDGKIFVTSVERVVRI
                     RTGEEDDAAV"
     gene            complement(5551..7215)
                     /locus_tag="PKV30_04010"
     CDS             complement(5551..7215)
                     /locus_tag="PKV30_04010"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRL29418.1"
                     /translation="MSLKIAVAQLNFIVGDMPGNAQKIIEAAKTAYEQGARLLLTPEL
                     SICAYICEDLFLRPSFMAACDEAVKTVARETAHLKDMAIVVGHPVGKDQRGKSVSVIK
                     RTNSASVIRDGRVIETYAKRLLPNYQLFDERRYFTPGQGTCVFQAGDVSVGLLICEDA
                     WFDEPAELAKEAGAELLAVMNASPFHVGKGYEREAMMIERVRASGLPLVYAHLVGGQD
                     EVVFEGQSFAVGADAQLVGRAPSFEEKLFFVEVERAQDALQLRATTAPARDNNADLWD
                     ALVLGLRDYVRKNGFKSVALGLSGGIDSALVLAVAVDALGQDAVHCVMMPSPYTAGIS
                     LEDAREMARRLGVRYDEISILPEFEAFKASLSPLFAGKPEDTTEENIQARIRGVMLMA
                     LSNKFGHMVLTTGNKSEYATGYCTLYGDMCGGFAPIKDVVKTKVFEMARWRNAHDPFK
                     TGANPIPERIITRPPSAELRADQTDQDSLPPYEVLDAIIERYMENDMGVAELVAEGFK
                     PDDVELVTRLIKINEYKRQQSAVGTRVTHRGFGKDWRYPMTNKFRA"
     gene            7376..8563
                     /locus_tag="PKV30_04015"
     CDS             7376..8563
                     /locus_tag="PKV30_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL29419.1"
                     /translation="MRLRSQFITDPADINPAAWNALLATQATPTPFMRHEYLAALHTS
                     GSAVARTGWAPHFLLLHEGDELAAACPLYVKAHSYGEYVFDWAWANAYAEHGLRYYPK
                     ALVAVPFTPVPGSRLLARDAAARAALVRALIDWCGQKKLSSLHVLFGDAADRAACDDA
                     GLMQRNQVQFHWQNTHPATGSPFASFDDFLASLTQEKRKKIRQERRKVMDAGVRFRWA
                     RGADIAAEDWAFFYRCYERTYLEHGNAPYLTPAFFAAMAQHMAEDWVLFIAERDGQSI
                     ASSLIAVGACPSSAGSPKGLNPVAYGRYWGALERVDCLHFEACYYQPIDWCIQHGVAR
                     FEGGAQGEHKMARALMPVATASSHWLAHPAFADAVERFLARESEGIEGYLAHLDERSP
                     FRH"
     gene            8643..9920
                     /gene="gltS"
                     /locus_tag="PKV30_04020"
     CDS             8643..9920
                     /gene="gltS"
                     /locus_tag="PKV30_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HRL29420.1"
                     /translation="MEIELNGYYTMILATLVLLLGRWLVVKIRFLSDFNIPEPVAGGL
                     LAGIVVFALNALFGLTFKFQGQLQTAFMLMFFSSIGLSADFSRLRAGGKPLVIFLFVV
                     AAFIMVQNLVGVGMAAALGLQPLIGLVTGSITLTGGHGTAGAWGPVLESRYGISGATT
                     LGIATATFGLVAGGLIGGPVAKYLIRKLDRHKLPQPTRQTKVEAVAASQSLYSTRHTE
                     GDTAPDNAMFDKPDEIRLITASSTIETLALFAGCMAFAGVMTTVAKGTVFELPTFVWA
                     LGGGVIIRNVLTHFFKFDMFDRAIDVFGNASLSLFLAMALLSLRLWELTSLALPVMVI
                     LAVQVCVMMAYAALVTFKVMGSDYDAAVLAAGHCGFGMGATPTAIANMQAITDRYGPS
                     YKAFLIVPMVGAFFVDLVNAAILQMFTQLPFLQ"
     gene            complement(10133..10453)
                     /locus_tag="PKV30_04025"
     CDS             complement(10133..10453)
                     /locus_tag="PKV30_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29421.1"
                     /translation="MKKTATLVCALTLAAGAQAACYTVMGPRGQILSETSTPPVDMRY
                     QLHQTVPYKYGRGASMVFGIADNNCGPEIDPYYDLMPTQVVYREGRRARVRHGMRAPR
                     RDRE"
BASE COUNT         1676 a         3592 c         3413 g         1846 t
ORIGIN      
        1 aagtaggtcg cccgccgggg cgaactcccg gccaaccgcg cccccaacac acgaatcgcg
       61 acaatcccca aagcgcccgc agcaagacgc gccgccctga atggaacccg ccttaacccc
      121 cctgctcgtc ctggcagccc tcgccaccgc cctcgccttc aaaccctggc gcctgctggc
      181 gaacacagcc ttactgacac cgctgctggc cgcgctcgtc atcaccccgt ggctgtgggc
      241 gctaccctgg ctgcaccaca tgcccatccg gctgcagctt tcgggggcct gcctcatcgg
      301 cctggccctc ggctggccgc tggcggtgcc ggcgctgtgc gcggtggcgc tcatcaccgg
      361 cctgatcgcg cccatcagcc tggcagaaca gatcgacatg gccctgtggc tcggcatcgt
      421 gcccgccacg ctctcgttcg gcctcggcat ggcgctgcgc cgctgggtgt cgaagaacct
      481 gttcgtctac atcctgggcc gcgcgttttt gggcaccgcg ctgtgcatgt ttgccagcgg
      541 cgcgctcgcc cactggaccg gccacgcgct ggcggccacg gtggagcctg aactggccct
      601 ggttgcacgc tggctgatcg cctggggcga cgccttcgtg accggcatga tggccgccat
      661 ctgcgtcgcc tttcgcccgc agtggctggc cacctggtca gatcggctct acgtggcccc
      721 acccgcgccg cccgcggcct gagcgccagc gccacaggca caatggcgcc cgccatgacc
      781 gctcgccgct ttcaccccct gcgccccttt ttgaccggca tgctggcgct gctgccgctg
      841 gccgccacgc tggtgctgct ggtgtgggcc acgcgcctgg cggccgaatg gctggggccg
      901 agcagcctgc tgggcagctt gctgacgcgc attggcgtcg gctttggccc cggcgccgcg
      961 ctgcccagct acctgatcgg gctgctggtg gtgctggcgc tggtctacgg ctttggcttg
     1021 ctggtcgaaa ccgggctgca gcgcgggctg gacagcgtga tccaggccat gatgcggcgc
     1081 atcccggtgg tgcgcacggt gtacgacatc gcgcaccggc tggtcggtct gttcagcaag
     1141 ccgaaggacg aaggcgtcaa gtcgatgcgc ccggtgtggc tgcattttgg cggcccgccc
     1201 gccgatggac agcctgccga ctcaacggtg gtgctgggcc tgctgaccac gcacgagccg
     1261 gtgctgctgg gcggccagcc ttttcatggc gtgctggtgc cgacggcgcc ggtgccggtc
     1321 ggcggcggcc tgctgttcgt gcccgcgcac tgggtgttgc cggcagatct cggcatcgag
     1381 gcggtgacca gcatctacgt ctccatgggc gtgacggcca accagtactt gggcgcgcaa
     1441 gccgcgccac cgccgcccga ccgcgcttga gtggcgtggt caccgccctt gcgcgggatc
     1501 aatacgcgcg gcacgccaaa agatcacact cagatcatcg cctctcatca gaaaggacgt
     1561 gatcgtgaaa ctttggcaag accttttcgg caccgactac gggttgatga gcatctccgg
     1621 cctgttgttc atcgtcttca tggcgttttg gttcgtgcgc ttctttctgc gcaagatggc
     1681 cgagccgcca ccgaagattt aagccttttt gagctccagc gcccgtccag tgtgcgccag
     1741 cagctatctt ttaaatagca atcagccgac cgattcggcc tcttgaagcg cgcgccacat
     1801 gaccttgccc gagccgctct tgggcagcgc atcggcaaac tgcaccacgc gcggcacctt
     1861 gtacacggcc atgttgtcgc ggcaccagtc gatgatgtcc tgctcgctca cctggccctg
     1921 gtggctgggg cgcagcacca ccacggcctt gaccgattcg ccgcggtagg catccttggt
     1981 ggcgatgatg caggcctcct gaatcgccgg gtggcggaac atcagcgcct ccacctcggc
     2041 cggccacacc ttgtagcctg acgcattgat catgcgcttg agccggtcgg tgatgaagaa
     2101 gtagccatcg tcatccaccc gccccagatc gcccgagcgg aagaagcgct tgccctccag
     2161 ttcgaaaaac gccgcctcgg tggcgtcggg ccgcttccag tagccttgaa aaacctcggg
     2221 tccatgaatc acgatctcgc cctgctcgcc ctgcggcacc tcttgcagcg tctcggggtc
     2281 gaccacgcgc gcatcggtgc tcatgaacgg gatgcccagg cactgctgct tggggtgatc
     2341 gaccgggttc tggtgcgaag gcgctgcggt ttcggtcagg ccgtagcctt cgcaataagc
     2401 cagcccgtac acgtcgagca gccgctgcgc caccgcctgc ggcatggcgg cgccgccgcc
     2461 gccaatcgtc ttcaggctcg acaagtcgaa gctggcgaaa ttggggctgg ccagcaggtc
     2521 gatcaccatg gtcgggatgt tgctccaggc cgtcacgcgc cagcgcgaaa tcaggcgccc
     2581 ggccagctcg cggtcccagc gcggcatgac caccatggtc gagccggcgt acaccgccgc
     2641 ctgcatgatg gccaccaggc cggtgatgtg gaacatcggc accaccacca ggccaatggt
     2701 ctccgacgtg ccaccgcccc acatcgcgca ggcgaccgcg ttgtgcatca cgctggcatg
     2761 cgtgtgcatg cagcccttcg gcaacccggt ggtgccgctg gtgtagggca ggatggccag
     2821 gtcggccggg ccggcggtgg tgtcggccag cgggccgccc caggccagcg cgtcttgcca
     2881 cgcatgcacc tggccgccag cgagcgcggg cagcgcgtgg tgcgtcagca gccagtcgcg
     2941 ccaggcggcg ggcggggcgt cgtcgccctc gatgttgccg tcaaagccgt cggtgaactg
     3001 ggtgacgatc aggtgctgca ggcgctgctc gggcggcagc gcatcgctgg cggcggccat
     3061 ctcgccggcc aggtcggcgg tggtgatggc gactttcgca tcggggtcgg tgatgtagtg
     3121 cttcagctcc tcggcgcggt tcatcgggtt caccggcacc accaccgcat cggcgcgcag
     3181 gatggcaaag tgcgccacga tgagctgcgg gcagttctgc atgttcagca ccacgcggtc
     3241 gcccttgctg acgcccagcg cctgcaactg cgccgccagc cgctcggcat cgtgcttgag
     3301 ctgcgcccag ctcatgacgc ggccgaagaa cacgatggcc gccttgtccg ggtagcgcat
     3361 ggcgctcacc gccaggtttt gccacagcga ggtggcgggc ggcgtgatgg cgtgcggcag
     3421 gcgcgcaggc cagaacttgt ggtgcgggcg catgggtgtt gtctcctgaa aaacggtgtt
     3481 cgcgcggcgc catcctagcg cggtgcaggc atgcaatggc ccgcgcattt gtcgccgcgg
     3541 cgacggcgcc gcgctagcat gcgggccgaa ccgtcaggag ccgccatgca cgccatcacc
     3601 ctcgcccgcc agttgctcgt cgccaccttg ctgggcgccg ccacactggc gcacgccttc
     3661 gacctgcaag gccaccgcgg cgcgcgcggg ctggcgcccg agaacacgct ggccgccttc
     3721 gaccgcgcgc tggccgaggg cgtgaacacg ctggagctgg acatcatcct cagcgccgac
     3781 ggcgtgccga tgatcgtgca cgacccgttt ctgccacccg acatgacgcg cgatgcacaa
     3841 ggccagtggc tgcccacgcc gacgccgctg atccgcacgc tgaccctcgc cgagctgcaa
     3901 agttacgacc tcggccgcgt gcggccggac agccgcacgg cgcgcgactt tccgcagcaa
     3961 aggccgcagg acggcgagcg aatgcccacg ctggcgcagc tgttcgagcg cgtgaaagcg
     4021 ctgggcgccg ccgacgtgcg cttcaacatc gagatcaaga ccaaccccga caagccgcaa
     4081 gacaccgtcg cgccagatca gttcgtcagc gccatcctgg ccgtggtgcg tgccgccgac
     4141 atgcagcagc gcgtgctgct gcaaagcttt gactggcgcc cgttgaagct ggcgcagcag
     4201 caggcacccg acatccccac cgcctacctc acggtcgaaa cccgcaacgc caacaacgcc
     4261 gccgcgcccg cgtggacggg cggcctgaag cgcaccgatt acccctccat cccccacatg
     4321 gtgcaagccg ccggcggcaa gctgtggacg cccaacttcg ccagcctgaa ccaagccgcg
     4381 ctgcggcagg cacagcaact gggcctgaaa gtgattccgt ggacggtgaa tgagcccgcc
     4441 gacatggcgc ggctgatcga ctggggcgtg gacggcctga tcaccgacta tccggaccgg
     4501 gcgcgcgccg tcatgcgcga caaaggcatg gcgctgccgg ctgcgattca aggcgccaag
     4561 actatcaatt gaatagctgc tcgcgcttgt tcctcaagcg ctggaggccg aaaaggcttg
     4621 aagcgcgcca agcatgaaaa ggcagcctcg gcgattttct ggttgtcatc gagggctttc
     4681 ttcggcgtgg ttggccctca ccccccttcg acaagctcag gacaggccaa gcctctctca
     4741 gagggagagg gagcaagaca atcctgcgtg aagcagcagg gtcgatggtt cacgggccga
     4801 gcgaagcgat ggcccggatg gacttcaaac ccccttctgg ccgtgccgag aagcgcaggg
     4861 cgtggggcgg gcgggcagcg cagcatgccc gcatcgtgct ctgactcgct gccgctgttt
     4921 gagcgaagag aacgcagtga acgcagcgag tccggcagcg ccgccccacg accgagcatc
     4981 gcaggcggcc cgcagggcca cggccagtgg ggccgccttt tctttggtta ctttcttttg
     5041 gcggagcaaa agaaagtgac tgcgccgccg ggcgcacatc ccggccgaca gcgcctccaa
     5101 tcaagcaccg ccagacaggc aaaaaaacct aaaccgccgc gtcgtcctct tcccccgtcc
     5161 gaatccgcac cacccgctcc accgacgtga caaaaatctt gccgtcaccg atcttgccgg
     5221 tgcgcgccgc cttgacgatc gcgtccacac aacgttcgac gtcgccctcg ctcaccacca
     5281 cctcgatctt caccttgggc agaaagtcca ccacgtactc ggcgccgcgg tacagctcgg
     5341 tgtgcccctt ctggcggccg aagcctttga cctcggtcac cgtcaggccg gtcacgccaa
     5401 cctcggccag cgcctcgcgc acctcctcca gcttgaaggg cttgatgacg gcggtgatct
     5461 gcttcatggc atgggctcct gtgcggattt gaatgaaatc aggcgccagc gcttgatggg
     5521 caagcgccag cagctatcaa cttcagagca tcaagccctg aacttgttgg tcataggata
     5581 acgccagtcc ttgccaaaac cgcggtgcgt cacgcgcgtg cccacggccg actgctggcg
     5641 cttgtattcg ttgatcttga tcaggcgcgt gaccagttcc acatcgtccg gcttgaagcc
     5701 ctcggccacc agctcggcca cacccatgtc gttttccatg tagcgctcga tgatcgcgtc
     5761 cagcacctcg tacggcggca ggctgtcctg gtcggtctgg tcggcgcgca gctcggcgct
     5821 gggcgggcgg gtgatgatgc gctcgggaat cgggttggcg ccggtcttga acgggtcgtg
     5881 cgcgttgcgc cagcgtgcca tctcgaacac cttggtcttc acgacgtcct tgatcggcgc
     5941 aaaaccgccg cacatgtcgc cgtacagcgt gcagtagccg gtggcgtatt cgctcttgtt
     6001 gccggtggtc agcaccatgt ggccgaactt gttcgacagc gccatcagca tcacgccgcg
     6061 gatgcgggcc tggatgtttt cctcggtcgt gtcctcgggc ttgccggcaa acagggggct
     6121 gagcgacgcc ttgaaggcct cgaactccgg caggatcgag atttcgtcgt agcgcacgcc
     6181 cagccgccgc gccatctcgc gcgcgtcttc caggctgatg ccggccgtgt agggcgaggg
     6241 catcatcacg cagtgcaccg cgtcctggcc cagcgcatcg acggcaaccg ccagcaccag
     6301 cgccgaatcg atgccgcccg acaggcccag cgccaccgac ttgaagccgt tcttgcgcac
     6361 gtagtcgcgc aggcccagca ccagcgcgtc ccacaaatcg gcattgttgt cgcgcgcggg
     6421 ggccgtggtc gctctcaatt gcagagcgtc ctgcgcacgc tccacctcga caaagaacag
     6481 tttctcttca aaactcggcg cccggccgac cagttgcgca tcggcaccca cggcaaacga
     6541 ctgtccttcg aacaccactt cgtcctgccc gcccaccagg tgcgcataga ccagcggcag
     6601 gccgctggcg cgcacgcgct cgatcatcat cgcctcgcgc tcgtagccct tgccgacgtg
     6661 gaagggcgag gcgttcatga cggccagcag ctcggcgcca gcctctttcg ccaactcggc
     6721 cggttcgtcg aaccaggcgt cttcgcagat cagcaggccc acgctcacgt cgccggcctg
     6781 aaacacgcag gtgccctgcc ccggcgtgaa gtaacgccgc tcatcgaaca gctggtagtt
     6841 gggcagcagc cgcttggcgt aggtctcgat cacgcgacca tcgcggatca cgctggccga
     6901 gttggtgcgc ttgatcaccg acacgctctt gccgcgctgg tccttgccga ccgggtggcc
     6961 gacgacgatg gccatgtcct tcaggtgcgc tgtctcgcgc gccaccgttt ttaccgcctc
     7021 gtcgcaagcg gccatgaaag aggggcgcag aaacaggtct tcgcagatgt aggcgcagat
     7081 cgacagctca ggcgtcagca gcagccttgc gccctgctca tacgcggttt tggccgcctc
     7141 gatgatcttt tgcgcattgc ccggcatgtc gccgacgatg aagttgagct gggcgacggc
     7201 gatcttgagg gacatgggcg aggcggcgag aggattgaga gccgcatggt aaccgcgctg
     7261 tccctgcgta tggcgacaac gccaaacccc tgcggcggtg ccggcgcggt cggatcgaca
     7321 taatcgaaac ctgccccgaa accgacccgg ccagcccacg cgctcgcccc gccctgtgcg
     7381 ccttcgctcc cagttcatca ccgaccccgc ggacatcaac cccgccgcct ggaacgcctt
     7441 gctggccacg caagcgacgc cgacgccgtt catgcgccac gaatacctgg cggcgctgca
     7501 caccagcggc agcgcggtgg cgcgcaccgg ctgggcgccg cattttctgc tgctgcacga
     7561 aggcgacgaa ctggccgccg cctgcccgct gtacgtcaag gcgcattcgt atggcgaata
     7621 cgtgttcgac tgggcctggg cgaatgccta tgccgagcac ggcctgcgct actacccgaa
     7681 ggcgctggtg gcggtgccgt tcacgccggt gccgggctcg cggctgctgg cgcgcgatgc
     7741 cgctgcgcgc gcggcactgg tgcgggcatt gatcgattgg tgcgggcaga aaaaactgtc
     7801 atcgctgcat gtgctgtttg gcgatgcggc cgaccgggcc gcgtgcgatg acgcggggtt
     7861 gatgcagcgc aaccaggtgc agtttcattg gcagaacacg catccggcga caggttcgcc
     7921 gttcgcgagc ttcgacgact tccttgcctc gctgacgcag gaaaagcgca agaagatccg
     7981 gcaggagcgg cgcaaggtga tggacgccgg tgtgcgcttt cgctgggcgc gcggggccga
     8041 catcgcggcc gaggattggg cttttttcta ccgctgctac gagcgcactt acctcgagca
     8101 cggcaacgcg ccctacctga cgccggcctt cttcgccgcc atggcgcaac acatggccga
     8161 agactgggtg ctgttcatcg ccgagcgcga cgggcagtcg attgcttcca gtttgatagc
     8221 tgttggcgct tgcccatcaa gcgctggcag cccaaaaggc ttgaacccag tggcctacgg
     8281 ccgctattgg ggggcgctgg agcgggtcga ttgcctgcac ttcgaggcct gctactacca
     8341 gcccatcgac tggtgcattc agcacggcgt ggcgcgcttc gagggcggcg cgcagggcga
     8401 gcacaagatg gcgcgcgccc tgatgccggt ggccacggcc agcagccact ggctggcgca
     8461 cccggcgttt gccgacgcgg tggagcgttt tctggcgcgc gagtccgagg gaatcgaggg
     8521 ctatctggcg cacctggacg agcgctcgcc gttcaggcac tgagcgcaga tggcggctca
     8581 agcggccgcg gcgccggcgc atgttttata actgcgccag aacgcctcag gaaaccgcga
     8641 cgatggaaat cgaactcaac ggctactaca cgatgattct ggccaccctg gtgctgctgc
     8701 tggggcgctg gctggtggtc aagatcaggt tcctgtcgga cttcaacatc cccgagccgg
     8761 tggccggcgg cctgctggcc ggcatcgtgg tgtttgcgct caacgcgttg ttcgggctga
     8821 ccttcaagtt ccagggccag ttgcagacgg cgttcatgct gatgttcttc tcgtccattg
     8881 gcctttccgc cgacttctcg cgcctgcgcg cgggcggcaa gccgctggtg atctttttgt
     8941 tcgtggtggc ggccttcatc atggtgcaga acctggtggg cgtgggcatg gcggcggcgc
     9001 tgggcctgca gccgctgatc ggcctggtca ccggctcgat cacgctgacc ggcggccacg
     9061 gcacggccgg cgcctggggg ccggtgctgg agagccgcta cggcatctcg ggcgccacca
     9121 cgctgggcat tgccaccgcc accttcggcc tggtggccgg cgggctgatt ggcggcccgg
     9181 tggccaagta cctgatcagg aagctcgacc gccacaagct gccccagccg acgcgccaga
     9241 ccaaggtgga agccgtggcg gcatcgcagt cgctctacag cacgcgccac accgagggcg
     9301 acaccgcgcc cgacaacgcc atgttcgaca agccggacga gatccggctc atcaccgcct
     9361 cgtccaccat cgagacgctg gcgctgtttg ccggctgcat ggcctttgcc ggcgtcatga
     9421 ccacggtggc caagggcacg gtgttcgagt tgcccacctt cgtctgggcg ctgggcggcg
     9481 gcgtgatcat ccgcaacgtg ctcacgcact tcttcaagtt cgacatgttc gaccgcgcca
     9541 ttgacgtatt cggcaacgcc tcgctgtcgc tgtttctggc catggcgctg ctgtcgctgc
     9601 ggctgtggga gctgacctcg ctggcgctgc cggtgatggt catcctggcg gtgcaggtgt
     9661 gcgtgatgat ggcctacgcg gcactggtca ccttcaaggt gatgggcagc gactacgacg
     9721 ccgccgtgct ggcggccggg cattgcggtt ttggcatggg cgccacgccg acggcaattg
     9781 ccaacatgca ggccatcacc gaccgctacg ggccgtcgta caaggcgttc ttgatcgttc
     9841 cgatggtggg cgcgttcttt gtcgacctgg tcaacgcggc gatcctgcag atgttcacgc
     9901 agctgccgtt tttgcaatag cgaacgggcg cggcgataca gccagaacca caaacaatcc
     9961 aaccccgttt gtcctgagcc tgtcgacgga cctggtgcaa gagaccgagg cttcgacaag
    10021 ctcagcccga acgggtgtgg ggtcgttgga ctatttgtgc gtcctgccat aaaaacccgc
    10081 cgcgctggca gcgcggcggg ttttttgctt ttcctcaatg tcgcaggacg acctactcgc
    10141 ggtcgcgccg cggcgcgcgc atgccgtggc gcacgcgggc acgccggcct tcgcggtaca
    10201 ccacctgcgt cggcatcagg tcgtaatacg gatcgatctc gggcccgcaa ttgttgtcgg
    10261 caatgccgaa caccatgctg gcaccgcggc cgtacttgta gggcacagtc tggtgcagct
    10321 ggtagcgcat gtcgaccggc ggcgtcgagg tttcggacag gatttgcccg cgcggcccca
    10381 tgacggtgta acaggcggcc tgcgcgccgg cggccagcgt cagggcgcaa acaagggtgg
    10441 cggtcttttt catggcgcta ttttgcagtt tcttacagat acgggccagt ctgccgcgct
    10501 ggcggcgctt gtcaagcgga cggcgcc
//
LOCUS       DAOMVO010000049        10493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1530_length_10493_cov_16.718528, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000049 DAOMVO010000000
VERSION     DAOMVO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_10493_cov_16.718528"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKV30_04030"
     CDS             <1..189
                     /locus_tag="PKV30_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRL29422.1"
                     /translation="ELNYDYGLVIDEPLTPELTAEYPCWCGSKNCRGTLLSSDLDTVE
                     GRGEKKPKKKKKKKAKKD"
     gene            193..990
                     /locus_tag="PKV30_04035"
     CDS             193..990
                     /locus_tag="PKV30_04035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRL29423.1"
                     /translation="MSAPTLSWPTDALRAQLAPLLPGIGVEVVGEIDSTNSELMRRAR
                     AGRTAPTLLVAELQTAGRGRLGRGWQSGDGEPGGALAFSLGLLLAPRDWSGLSLAVGL
                     AVAEALHLDIRLKWPNDLWWQGRKLAGILVETAGTGGGHSDRFAVIGIGINIAPLGGE
                     GFSTPPAWLQALLPGITAPDALMRLAAPLVQAVRAFEPQGFAPLAARFNARDALGDQA
                     VRLSDGTEGVARGVGADGALQVHTATGLRTVHSAEVSVRPMTGEGAR"
     gene            992..1177
                     /locus_tag="PKV30_04040"
     CDS             992..1177
                     /locus_tag="PKV30_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29424.1"
                     /translation="MKHDFASLNDLVLQPSMTVLRAMTEVMRPTGASSKAQAFGHAPQ
                     ARSSLHALRAVRSAMDS"
     gene            1179..1913
                     /locus_tag="PKV30_04045"
     CDS             1179..1913
                     /locus_tag="PKV30_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL29425.1"
                     /translation="MVGRLVVLLLIANAAWFAWSQGWLRVLGLAPTVQSEPERMQRQV
                     RPEALTLRPLDAASAAAPRAVAAAETPAPVAPPPVAVTEAPPAAAAPPAPAGICLQAG
                     VFDEKQIEPVRRAAASLPAGSWRIDSVQLPGRWMVYVGKLADADAVRTKRGELRELGV
                     DTDRPGAALEPGLSLGRFSNEEAAQRALTDLGRKGVRTARVVQERRDTPAYMLRLPSA
                     DAALRQQARGLRGALGGKDLRECDPA"
     gene            2117..2776
                     /locus_tag="PKV30_04050"
     CDS             2117..2776
                     /locus_tag="PKV30_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRL29426.1"
                     /translation="MTLKLYYASGACSFVPHTMLEMAGATFEPVAVKLHKGEQNSPEY
                     QAINPRGQVPVLADGDEVITQIMAIIAYIDARFPENAFIPKDPLARARFVETLAWMNN
                     TVHPTFTHVFMPHKFSDDEAAQGQMKRFGVGNYRKLMDELQGLVKKAHAAGHDWLGGV
                     HAGPLDAYALTLLRWGTIAGINPEDYPELWAHVQRTALVPAVARAMERERLQLNLYKP
                     A"
     gene            complement(2858..4315)
                     /locus_tag="PKV30_04055"
     CDS             complement(2858..4315)
                     /locus_tag="PKV30_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HRL29427.1"
                     /translation="MAVKIFQRNQRSLFGEILDWMLTPLLLLWPISLALTWLVAQNLA
                     NKPFDRALVYNAQALAQFIQPGPNHRLRFSLPQPASELLRADDADNVYYQVLGPKGEF
                     LSGERELPPPAPLEGDEGARQAFDEPHLRDAEFRGLPVRIAHLWVPVADSATPALVQV
                     AETREKRSVLAAEIIKGVMLPQFAILPLAVLLVWLALVRGIKPLSQLELRIRERRPDD
                     LSPLDDHAVPQEVAPLVGSVNDLLTRLKDSIATQKRFLADAAHQLKTPLAGLSLQADL
                     AQREGASAGDLKQSLQQIGHASERATHTVNQLLALARAEAGGAAMAQQRCDLAALTIE
                     VVREAVPRAFDRQIDLGYEGPEPGAPELVLQGNPTLLKEMIRNLVENALHYTTSTPAA
                     PGHVTVRLQSDPFSRALVLQVEDNGPGVPAAEREAVFQPFYRALGTNVHGTGLGLPIV
                     REIAAQHRAVVDLDDAHPGQQPPGARFTVRFEAPD"
     gene            complement(4518..5192)
                     /locus_tag="PKV30_04060"
     CDS             complement(4518..5192)
                     /locus_tag="PKV30_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL29428.1"
                     /translation="MRILIAEDDQVLADGLMRSLRGSGAAVDHVANGSEADAALLTNT
                     EFDLLILDLGLPKMHGLEVLKRLRARGQTLPVLILTAADSVEERVKGLDYGADDYMAK
                     PFSLQELEARVRALTRRGMGAASATIKHGPLEYDQTGRVASIDGKMVDLSARELGLLE
                     VLLQRSGRLVSKEQLVERLCEWGEEVSNNAIEVYIHRLRKKIEKGPIRIATVRGLGYC
                     LEKIPP"
     gene            complement(5339..5833)
                     /locus_tag="PKV30_04065"
     CDS             complement(5339..5833)
                     /locus_tag="PKV30_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRL29429.1"
                     /translation="MRDEPLQPPPDDRWRQTHLGRLLGHAMRRFDQRVLQLMAASAEA
                     PLALSHLAARDKVGAAHIHLTRHLPLEGARLTELAQSAGMTKQAMGDLVDQCVAWGIV
                     AREDDPRDARARRIVFTPTGLDWLAAFRDAVAQAEAEFRAAVGDEVATVVALGLEAYA
                     EGAT"
     gene            5964..7022
                     /gene="recA"
                     /locus_tag="PKV30_04070"
     CDS             5964..7022
                     /gene="recA"
                     /locus_tag="PKV30_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRL29430.1"
                     /translation="MDMAVKSDNSEKAKALQAALAQIEKQFGKGTIMRLGEGEAIEDI
                     QVVSTGSLGLDIALGVGGLPRGRVIEIYGPESSGKTTLTLQVIAEMQKLGGTCAFIDA
                     EHALDTGYAEKLGVNLHEMLISQPDTGEQALEIVDSLVRSGAVDLVVIDSVAALTPKA
                     EIEGEMGDSLPGLQARLMSQALRKLTATIKKTNCTVIFINQIRMKIGVMFGSPETTTG
                     GNALKFYASVRLDIRRTGTIKKGDVAIGNETKVKVVKNKVSPPFKTAEFDILFGEGIS
                     REGEIIDMGANAKVLDKSGAWYAYNGEKIGQGRDNAREFLRENPDLAREIENKVRESL
                     GIAERPAALAGGAEADEA"
     gene            7105..7575
                     /gene="recX"
                     /locus_tag="PKV30_04075"
     CDS             7105..7575
                     /gene="recX"
                     /locus_tag="PKV30_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HRL29431.1"
                     /translation="MTSGARVPSLKGRALRYLAAREHSRAELQAKLARHVTYEDEPGA
                     LTRVLDELAAKGFINEARVAESLLHRRAGRLGQARVLQELRAKGLPDEIMADAADQLR
                     TTELARAREVWRKKFGQVPVDAADKARQMRFLAGRGFGAETARRVLAGGYDEDV"
     gene            7791..8339
                     /locus_tag="PKV30_04080"
     CDS             7791..8339
                     /locus_tag="PKV30_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29432.1"
                     /translation="MTIVYALSPKGERECAADPPTLPAELSGLLRMVDGHRTRQDLLR
                     ATEKSALTAGGLRWLSDAGYIRPASDAAPQPGKQGARPALTPLPALPSSAAPEQSRSE
                     ADVRQTLAEFMVKGIRRHLGEAGYPQRRQIERAKAVGDLLPHLYPLVDAILERVGSEA
                     AAEFADTAAFILHPLERDGLLN"
     gene            complement(8463..>8827)
                     /locus_tag="PKV30_04085"
     CDS             complement(8463..>8827)
                     /locus_tag="PKV30_04085"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867932.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRL29433.1"
                     /translation="IMKYGRLTRRPIFVPAVGNFDQGMLVELPLHLDLLPGKPTAAQL
                     LAAYRAHYAGAGAVTVHDAPESGKLDAVALKNTNELEIRVFGNPQAAQAVAVARLDNL
                     GKGASGAAVQNLKLMLGL"
     assembly_gap    8828..8927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8928..9529)
                     /gene="argC"
                     /locus_tag="PKV30_04090"
     CDS             complement(<8928..9529)
                     /gene="argC"
                     /locus_tag="PKV30_04090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRL29434.1"
                     /translation="MAKVFIDGEAGTTGLQIRERLADMPDVDLVSIDPAKRKDANAKR
                     EIMAGVDLVILCLHDDAARESVAMIDALPGKKPRIIDASTAHRVAEGWTFGFPELSKD
                     QRAAVQQAERVANPGCYATGAIALLRPLVDAGLLPADHPTVLPSVSGYTGGGRQMIEA
                     YEAGNAPAYELYALGLQHKHIPEIMKYGRLTRRPIFVPAVG"
     gene            9706..>10493
                     /gene="sucC"
                     /locus_tag="PKV30_04095"
     CDS             9706..>10493
                     /gene="sucC"
                     /locus_tag="PKV30_04095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRL29435.1"
                     /translation="MKIHEYQGKEILRQFGVPVPRGIPAFTVQEAVEAAQKLGGPVWV
                     VKAQIHAGGRGKGGGVKVAKSIDEVKKLAGEILGMQLKTHQTGPEGQKVRRLYIEDGA
                     DIQKEYYVSVVTDRGTQKVAFIASSEGGMDIEEVAHSHPEKIIKVFVDPLVGMTDADA
                     KELADGIGIPADSTAQAVDVFKKLYKCYMDTDASLVEINPLNRDSKGNIMALDSKFNF
                     DSNALYRHPEIVALRDLDEEDPAEIEASKFDLAYISLDGNIGCLV"
BASE COUNT         1713 a         3502 c         3524 g         1654 t
ORIGIN      
        1 gagctgaact acgactacgg cctggtcatc gacgagccgc tgacgcccga gctgacggcc
       61 gaatacccct gttggtgcgg cagcaagaac tgccgcggca cgctgctgtc gtccgacctc
      121 gacaccgtcg aagggcgcgg cgagaaaaag cccaagaaga aaaagaagaa gaaagccaag
      181 aaggactgac gcatgagcgc ccccaccctg agctggccca ccgacgccct gcgcgcgcag
      241 ctggcgccgc tgctgccggg catcggggtc gaggtggtgg gcgaaatcga ctccaccaac
      301 agcgagctga tgcgccgcgc gcgcgctggc cgcacggcgc ccacgctgct ggtggccgaa
      361 ctgcagaccg ccgggcgtgg ccgcctgggc cggggttggc agtcgggcga cggcgagccg
      421 ggcggcgcgc tggcgttttc gctcggtctg ctgctggcgc cgcgcgactg gtcgggcttg
      481 tcgctggccg tgggtctggc cgtggccgag gcgctgcacc tggacatccg cctgaaatgg
      541 ccgaacgatt tgtggtggca gggccgcaag ctggccggca tcctggtcga gaccgcgggg
      601 acgggcggag ggcacagtga ccgctttgcc gtcatcggca tcggcatcaa catcgcgccg
      661 ctgggcggcg aaggtttttc caccccgccc gcctggctgc aggcgctgct gcccggcatc
      721 acggcgcccg acgcgttgat gcgcctggcc gcgccgctgg tgcaggcggt gcgcgcgttc
      781 gagccgcagg gctttgcccc gctggcggcg cgcttcaacg cccgcgacgc gctgggcgat
      841 caggccgtgc gcctgtccga cggcaccgag ggcgtcgcgc gcggcgtggg cgccgatggc
      901 gcgctgcagg tgcacaccgc caccggtctg cgcaccgtgc acagcgccga agtcagcgtg
      961 cggccgatga caggcgaagg ggcgcgctga aatgaaacat gacttcgctt cactcaatga
     1021 tcttgtcttg cagccttcga tgacggtgct gcgcgccatg actgaagtca tgcgcccgac
     1081 gggcgcgtca tcaaaggcgc aggcctttgg tcatgcgccg caggcgcggt catcgttgca
     1141 tgcattgcgg gcggtgcgca gcgccatgga tagctgacat ggttgggcgc ctggttgttc
     1201 ttctgctgat cgccaatgcg gcctggtttg cctggtcgca aggctggctg cgcgtgctgg
     1261 gcttggcgcc cacggtgcag agcgagcccg agcggatgca gcgccaggtg cggcccgagg
     1321 cgctgacgct gcggcctttg gacgccgcct cggcggccgc gccgcgcgcc gtcgcggctg
     1381 ccgagacgcc ggcaccggtg gccccgccgc ccgtcgccgt gaccgaggcg ccccctgctg
     1441 cggctgcgcc gcctgcaccc gccggcatct gcctgcaggc cggcgtgttc gatgaaaagc
     1501 agatcgagcc ggtgcgccgc gccgccgcca gcctgccggc cggcagttgg cgcatcgatt
     1561 cggtgcagct gcccgggcgc tggatggtct acgtcggcaa gctggccgat gccgacgcgg
     1621 tgcgcaccaa gcgcggcgag ctgcgagagc tgggcgtgga caccgaccgc cccggcgccg
     1681 cgctggagcc cggcctgtcg ctgggccgct tttccaacga agaagcggcc cagcgcgcgc
     1741 tgaccgattt gggccgcaag ggggtgcgca ccgcccgcgt ggtgcaggag cggcgcgaca
     1801 cacccgccta catgctgcgc ctgccgagtg ccgacgccgc gctgcgccag caggcgcgcg
     1861 ggctgcgcgg ggcgttgggg gggaaagacc tgcgcgagtg tgaccccgcc tgaggcgcct
     1921 gcggcgttcc cgaacggcct gctgcgcggc gcccgtggca acaagtcctg atttgatagc
     1981 tgcgtgcgct tgatacataa gcgctggacg ctactttcga tctgattgtc gtggccgtga
     2041 tcgttgccgc cgcgcggcgc cttccgcatc tggcgctatg ctcgtgaccg ccccgaccga
     2101 ttctcaggag acccgcatga ccctcaagct ctactacgcc tctggcgcct gctccttcgt
     2161 gccgcacacc atgctcgaaa tggctggcgc cacgttcgag ccagtggccg tcaagttgca
     2221 caagggcgag cagaacagcc ccgaatacca ggccatcaac ccgcgcggcc aggtgccggt
     2281 gctggccgat ggcgatgagg tgatcacgca gatcatggcc atcatcgcct acatcgacgc
     2341 gcgttttccc gaaaacgcct tcatccccaa ggacccgctg gcgcgcgcgc gcttcgtcga
     2401 aacgctggcc tggatgaaca acaccgtgca ccccaccttc acgcacgtct tcatgccgca
     2461 caagttcagc gacgacgagg cagcgcaggg ccagatgaag cgctttggcg ttggcaacta
     2521 ccgcaagctg atggacgagc tgcaaggcct ggtgaagaaa gcgcacgcgg ccggccacga
     2581 ctggctgggc ggtgtgcacg ccggcccgct cgacgcctac gcactgacgc tgctgcgctg
     2641 gggcaccatt gccggcatca accctgagga ctacccagag ctgtgggcgc acgtgcagcg
     2701 caccgcgctg gtgcccgccg tggcgcgcgc catggagcgc gagcggctgc agttgaattt
     2761 gtacaagccg gcttgagcgc agcgaagcca tgcgttatgg gctgccagcg cttgtccagc
     2821 gtgcgcgagg cgctatcaaa aatgacgata tgtgccatca atccggcgcc tcgaagcgca
     2881 ccgtaaaccg cgcccccggc ggctgctggc ccgggtgcgc atcgtccaga tcgaccaccg
     2941 cgcggtgttg cgcggcgatc tcgcgcacga tcggcaggcc gaggccggtg ccgtgcacgt
     3001 tggtgccgag cgcgcggtaa aacggctgaa acaccgcctc gcgctcggcc gccggcacgc
     3061 cggggccgtt gtcttccacc tgcagcacca gcgcgcggct gaacgggtcg ctttgcagcc
     3121 gcaccgtgac gtggcccggc gctgcgggcg tcgacgttgt gtagtgcagc gcgttttcca
     3181 ccagattgcg gatcatctcc ttcagcagcg tcgggttgcc ttgcagcacc agctcgggcg
     3241 cgccgggctc ggggccttcg tagcccaggt cgatctggcg gtcgaaggcc cgcggcacgg
     3301 cttcgcgcac cacctcgatg gtcagcgcgg ccaggtcgca gcgctgttgt gccatcgccg
     3361 cgccgccggc ttcggcgcgg gccagcgcca gcagctggtt gacggtgtgc gtggcgcgct
     3421 cgctggcgtg gccgatttgc tgcagcgact gcttgaggtc gcccgcgctg gcgccttcgc
     3481 gctgcgccag gtcggcctgc agcgacagcc cggccagcgg cgtcttgagc tggtgcgcgg
     3541 cgtctgccag aaagcgcttt tgcgtggcga tggaatcctt cagccgcgtc agcaagtcgt
     3601 tgaccgagcc gaccagcggc gccacttcct gcggcacggc gtggtcgtcc aacgggctca
     3661 aatcgtccgg gcggcgctcg cggatgcgca attcgagctg cgacagcggc ttgatgccgc
     3721 gcaccagcgc cagccacacc agcagcaccg ccagcggcag gatggcgaac tgcggcagca
     3781 tcacgccctt gatgatttcg gccgccagca cgctgcgctt ttcgcgcgtt tcggccacct
     3841 gcaccagcgc cggcgtggcg ctgtccgcca ccggcaccca caggtgggcg atgcgcaccg
     3901 gcaggccgcg gaattcggca tcgcgcaggt gcggctcgtc gaaggcctgg cgcgcgccct
     3961 cgtcgccttc gagtggcgca ggcggcggca gctcgcgctc gcccgagagg aattcgccct
     4021 tcggccccag cacctggtag tagacgttgt cggcgtcgtc ggcgcgcagc agctcgctgg
     4081 ccggctgcgg cagcgaaaag cgcagccggt ggttgggccc cggctggatg aactgcgcca
     4141 gcgcctgcgc gttgtagacc agcgcgcggt cgaacggctt gttggccagg ttctgcgcca
     4201 ccaaccacgt cagcgccagg ctgatcggcc acagcagcag caagggcgtc agcatccagt
     4261 ccaggatttc gccgaacagc gagcgctggt tgcgctgaaa aatcttgacg gccatgctgc
     4321 gtattttata gctaccggcg ctttcccctc aagcgctggc agcccatttc attcaaatcg
     4381 tgcgcaccag aaagcgggct gcgcagcaag cccgctggta ttcaagtggc cgcggagcag
     4441 gccattcggg aacgccgtgg ccggggtccc cccggtcacc agcgttgtcc ccctgggggg
     4501 aaggcgccgc aggcgactca ggggggaatc ttctcgaggc agtaacccaa accccgcacc
     4561 gtcgcgatgc ggatcggccc cttctcgatc ttcttgcgca ggcggtggat gtagacctcg
     4621 atcgcgttgt tgctcacctc ttcgccccat tcacacaggc gctcgaccag ctgctccttg
     4681 ctcaccagcc gccccgaacg ctgcagcaac acctccagca gccccagctc gcgcgccgac
     4741 aggtcgacca tcttgccgtc gatgctggcc acgcggccgg tctggtcgta ttcgagcggg
     4801 ccgtgcttga tggtggcgct ggccgcgccc atgccgcgcc gcgtcagcgc ccgcacgcgc
     4861 gcttccagct cctgcaggct gaagggcttg gccatgtagt cgtcggcgcc gtagtccagg
     4921 cccttgacgc gctcttccac gctgtcggcg gccgtgagga tcagcaccgg cagcgtttgc
     4981 ccgcgcgcgc gcagcctctt taacacctcc agcccgtgca tcttgggcag gccgaggtcg
     5041 aggatgagca ggtcgaactc ggtgttggtc agcagcgcgg cgtcggcctc gctgccgttg
     5101 gccacgtggt cgaccgccgc gcccgagccg cgcaggctgc gcatcagacc gtcggccagc
     5161 acctgatcgt cttcggcgat gaggatgcgc atgggttgtc tcctaatgtg gcgctgcctg
     5221 tggtgtgccg gcgccccgcg ccgtgtccct tgcgttccat tctaggagtt gcgccggccg
     5281 cggtaagcag gtgtgacccg gatgccggtg cgcgcgctgc ctggtcgccc atgcgctttt
     5341 aggtcgcccc ttcggcatac gcctccagcc ccagcgccac caccgtcgcc acctcgtcgc
     5401 cgacggcggc gcgaaactcg gcctccgcct gcgccaccgc gtcgcgaaag gccgccagcc
     5461 aatccagccc tgtcggcgtg aagacgatgc gccgcgcgcg ggcgtcgcgc gggtcgtcct
     5521 cgcgcgcgac gatgccccag gcgacacact gatcgaccag atcgcccatg gcctgcttgg
     5581 tcatgcccgc cgactgcgcc aactcggtca ggcgggcgcc ttcgagcggc aggtggcgcg
     5641 tcaggtggat gtgcgcagcg cccaccttgt cgcgcgcggc cagatgcgac agcgccagcg
     5701 gtgcctcggc gctcgcggcc atcaactgca gcacgcgctg gtcaaaccgt cgcatggcgt
     5761 ggcccagcag gcggccgagg tgggtctggc gccagcggtc gtcgggcggc ggttgaagcg
     5821 gctcgtcacg catccccaaa tcgtaaggca aactgaccta aaaatctgtt tacgacaatc
     5881 aatggattcc tatacagtcc tggtcaagca tccagtcaaa tcacgccagt gatgcgcaac
     5941 gtattgattt aaaaggaaga atcatggaca tggcggtcaa gagcgacaac agcgaaaaag
     6001 ccaaggccct gcaagccgcg ctggcgcaga tcgaaaagca gttcggcaag ggcaccatca
     6061 tgcgcttggg cgagggcgag gcgatcgagg acatccaagt cgtttccacc ggctcgctgg
     6121 ggctcgacat cgccctgggc gtgggcggcc tgccgcgcgg ccgggtgatc gaaatctacg
     6181 gccctgaatc gtcgggcaag accacgctga cgctgcaggt cattgccgaa atgcagaagc
     6241 tgggcggcac ctgcgccttc atcgacgccg agcacgcgct ggacaccggc tacgccgaaa
     6301 agctgggtgt gaacctgcat gaaatgctga tcagccagcc cgacaccggc gagcaggcgc
     6361 tggaaatcgt cgattcgctg gtacgctcgg gcgccgtcga tctggtggtc atcgactcgg
     6421 tggcggcgct cacgcccaag gccgagatcg aaggcgaaat gggcgattcg ctgcccggcc
     6481 tgcaggcgcg cctgatgagc caggcgctgc gcaagctcac cgccaccatc aagaagacca
     6541 actgcacggt gatcttcatc aaccagatcc gcatgaagat cggcgtcatg ttcggcagcc
     6601 ccgagacgac gaccggcggc aacgcgctga agttttacgc cagcgtgcgc ctggacatcc
     6661 gccgcaccgg caccatcaag aagggcgacg tggccatcgg caacgagacc aaggtgaagg
     6721 tggtcaagaa caaggtcagc ccgcccttca agacggccga gttcgacatc ctgtttggcg
     6781 aaggcatcag ccgcgagggc gagatcatcg acatgggcgc caacgccaag gtgctcgaca
     6841 aatccggcgc ctggtacgcc tacaacggcg aaaaaatcgg ccaggggcgc gacaacgcgc
     6901 gcgagtttct gcgcgagaac cccgatctgg cgcgagaaat cgagaataaa gtgcgcgagt
     6961 ctctcggtat cgccgagcgt ccggcggcgc tggcgggtgg ggctgaggcg gacgaggcct
     7021 gagggcggaa gaatatcaat gaaaagtggc tctggcgctt gtccatcaag cgcaagcagc
     7081 tattgaaaac agagtgatca gtcgatgact tctggcgccc gcgtcccctc cctcaaaggc
     7141 cgtgcgctgc gctacctggc cgcgcgcgag cattcgcggg ccgagctgca ggccaagctg
     7201 gcgcgccacg tcacctacga ggatgagccc ggcgcgctga cccgcgtgct ggacgagctg
     7261 gcggccaagg gcttcatcaa cgaggcgcgc gtggccgaat cgctgctgca ccgccgcgcc
     7321 gggcgcctgg gccaagcgcg cgtgctgcaa gagttgcgcg ccaaggggct gccggacgag
     7381 atcatggccg acgcggcgga ccagctgcgc accaccgaac tggcgcgcgc gcgcgaggtg
     7441 tggcgcaaga aattcggcca agtgccggtc gacgcggccg acaaggcacg ccagatgcgc
     7501 tttctggccg ggcgcggctt tggcgccgag acggcgcgcc gggtgctggc gggcgggtac
     7561 gacgaggacg tttgacggcg agccttgggt cgtgccgacc ggtcggcggt ccggctccgc
     7621 cgcatcgcgc ttggtgaata gtgcacacga tttcccccca attgggtatt gacgacccgc
     7681 tgcctgaagc attccaatag caaccgtccc agctgtttct aaatgttgtt ttcggtaacc
     7741 gccaactgag cgtcgataca gcaggccggg gcgaatcagg gagagtcaaa atgaccatcg
     7801 tctatgcctt gagccccaag ggcgagcgcg aatgcgcggc cgatcccccc accctgcctg
     7861 ccgagctgag cggcttgttg cgcatggtcg atggccatcg cacccggcaa gacctgctgc
     7921 gcgccaccga gaaaagcgca ttgacggccg gcggcttgcg ctggctgagc gacgccggtt
     7981 acattcggcc ggcctccgac gctgcgccgc agccgggcaa acagggggct cggccggcgc
     8041 tgacgccctt gccggcactg ccgagttcgg ccgcacctga gcagtcacgc agcgaagccg
     8101 acgtgcgcca gaccctggcc gaattcatgg tcaagggcat tcgccggcat ctgggtgaag
     8161 cgggttaccc gcaacgccgc caaatcgagc gcgccaaggc ggtgggcgat ttgctgccgc
     8221 acctgtaccc gctggtcgat gcgatcctgg agcgcgtggg cagcgaagcc gcggccgagt
     8281 ttgccgacac cgccgcgttt atcttgcacc cgctggagcg cgacgggctg ctgaactgag
     8341 gcgactgccg tcacatgatg acgggcagtt gctctaaact ttatagctgg ttgcgcaaat
     8401 ctgacgcgcg ccacgccgct atttgactcg aattttcagc gctgccccgc gcgccgcagc
     8461 gttcaaagcc cgagcatcag cttgaggttc tgcaccgccg cgccgctggc gcccttgccc
     8521 aagttgtcca gccgcgccac ggccaccgct tgggccgctt gggggttgcc gaagacgcga
     8581 atttccagct cgttggtgtt cttcagcgcc accgcgtcga gcttgccgga ttccggcgcg
     8641 tcatgcactg tcacggcccc ggcgccggca tagtgcgcgc ggtaggccgc cagcaattgc
     8701 gcggcggtcg gtttgcccgg cagcaggtcg aggtgcagcg gcagttccac cagcatgccc
     8761 tggtcgaaat tacccaccgc cggcacgaag atcggccggc gcgtgaggcg gccgtatttc
     8821 atgatctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc accgccggca
     8941 cgaagatcgg ccggcgcgtg aggcggccgt atttcatgat ctcgggaatg tgcttgtgct
     9001 gcaggccgag cgcgtacagc tcataggccg gcgcgttgcc cgcttcgtag gcctcgatca
     9061 tctggcgccc gccgcccgtg tagccgctga ccgaaggcag cacggtgggg tggtccgccg
     9121 gcagcagccc ggcatcgacc agcggccgca gcagcgcgat ggcgccggtg gcgtagcagc
     9181 cggggttggc cacgcgctcg gcttgctgca cggcggcgcg ctggtctttc gacaactcgg
     9241 ggaagccgaa agtccagcct tcggccacgc ggtgcgccgt gctggcgtcg atgatgcgcg
     9301 gcttcttgcc cggcagcgcg tcgatcatgg ccaccgattc gcgtgccgcg tcgtcgtgca
     9361 ggcacaggat gaccagatcg acaccggcca tgatctcgcg cttggcgttg gcgtccttgc
     9421 gtttggccgg gtcgatgctg acgagatcga cgtccggcat gtccgccagc cgctcgcgaa
     9481 tctgcagccc ggtggtgccg gcttcgccgt cgatgaaaac cttggccatt gcgtgttgtc
     9541 ctttgcgtgg cgccgcgtca tggcaacgta agcccgggcg cgagaattgg tgcgtcgcac
     9601 catgatacat tcgtgcgctg cggcccgcat tcccgtcgtc ctgacgcgcc aagcgccgtc
     9661 tgggttgcgt cggccgaccg ttcacacacc actccagagg gcttcatgaa gatccacgag
     9721 taccagggca aggagatcct gcgtcaattc ggcgtgcccg tgccccgcgg catcccggca
     9781 ttcaccgttc aagaggccgt cgaagcggcg caaaagctgg gcggccccgt gtgggtggtc
     9841 aaggcgcaga tccacgccgg cggccgcggc aagggcggcg gcgtgaaggt cgccaagtcg
     9901 attgacgagg tgaaaaagct ggccggcgaa atcctgggca tgcagctcaa gacgcatcag
     9961 accgggcctg aaggccagaa agtgcgccgc ctgtacatcg aggacggcgc cgatattcaa
    10021 aaagagtatt acgtctcggt cgtcaccgac cgcggcacgc agaaggtggc cttcatcgcc
    10081 agcagcgaag gcggcatgga catcgaggag gtggcgcaca gccaccccga gaagatcatc
    10141 aaggtcttcg tcgatccgct ggtcggcatg accgatgccg atgcgaagga actggccgac
    10201 ggcatcggca ttcccgccga ctccaccgcg caggccgtgg acgtgttcaa gaagctctac
    10261 aaatgctaca tggacaccga cgccagcctg gttgagatca acccgctgaa ccgcgacagc
    10321 aagggcaaca tcatggcgct ggactcgaag ttcaacttcg acagcaacgc cctgtaccgc
    10381 caccccgaga tcgtggccct gcgcgatctg gacgaagaag atccggccga gatcgaagcc
    10441 agcaagttcg acctggccta catcagcctg gacggcaaca tcggctgcct ggt
//
LOCUS       DAOMVO010000050        10431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1545_length_10431_cov_16.948439, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000050 DAOMVO010000000
VERSION     DAOMVO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_10431_cov_16.948439"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKV30_04100"
     CDS             <1..292
                     /locus_tag="PKV30_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRL29436.1"
                     /translation="RSPAAPDIPTLSEAGLPGFEAVSWFALFAAPGTPKAVADKLSVQ
                     VAEVLKQPDVAKKLSDLGLEPVGSRPDELARYQQTEIAKWAKVVKESGATVE"
     gene            complement(315..1064)
                     /locus_tag="PKV30_04105"
     CDS             complement(315..1064)
                     /locus_tag="PKV30_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRL29437.1"
                     /translation="MTDRLAAAPTTCPSCRAPMQAHPVAAHHGATLELDLCHACHGIW
                     FDGNENLRMAPQGVVDLFRDLHAHRDDAQGPLSRDLNCPRCKRGLVQGADVVRTGRYI
                     TYRCPQRHGRFSTFSSFMIEKGFVRQLTKPEIADLAERVRIIHCGSCGAPVDLRQHDA
                     CPYCRAAFSLLDPQAVDRALAGYGQQARRADEGPKPTDLADALVALERDRERAKREEQ
                     KERWSNADASGFTVDDLWSAGVDLVWNVLRR"
     gene            1130..1852
                     /locus_tag="PKV30_04110"
     CDS             1130..1852
                     /locus_tag="PKV30_04110"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRL29438.1"
                     /translation="MPRFHVALPLSTDAELALPPGAARHVQVLRLQPGGAITVFDGTG
                     GEWDATITRMGRSDVQVRVGAHHAVERESTRAVHLVVGLMAAERMDWLVEKATELGAA
                     SLTPVLMARSSLRLSGERAEKKRAHWQAVSNAACEQSGRNRLLDVREQLTFQEYSRNA
                     TNATPARWVLSLAPDARPVAGCAAALSPTDDVTMVSGPEGGLSSEEDAAARAAGWQPI
                     TLGARVLRAETAPLAVLARLTL"
     gene            complement(1872..2144)
                     /locus_tag="PKV30_04115"
     CDS             complement(1872..2144)
                     /locus_tag="PKV30_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRL29439.1"
                     /translation="MQPLQDLQPHDHSTPEAIYGFDARGVAKRFRHAAIFGALDALQP
                     GEAMRFVNDHDPLPLLAQMQQRYGDGVRIEYRQRAPEGVMIDFIRQ"
     gene            2333..3514
                     /locus_tag="PKV30_04120"
     CDS             2333..3514
                     /locus_tag="PKV30_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL29440.1"
                     /translation="MNRALLLLTLCQGLFLTNNVTFIAINGLVGLQLAPFGWMATLPV
                     MGYVVGGALATPLVARTQARFGRKGSFQIGLVVALGSALLAAFAAWTRQFWLLVAATL
                     IAGYYSANGQLYRFAAAELAPPAWREKAVSLVLAGGLLGAIAGPNLAAWTRTVLPVPF
                     VGAYLALALVALLAMGVMAFIAFPPTPPKDAGSAAGRPLAEIMRQPVFMVATVGAALG
                     YGVMNLLMAATPLAMQVCGFDFGAAAQVLQWHVIGMFAPGFFTGHLIKRFGTLPIMGV
                     GVLLNGVCVAIALSGVDLQQFLVALFALGVGWNFLFTGSTTLALTAYRPEEKDRAQAA
                     INFCVFATMAFTSFASGALVTTQGWRWLNLGSLPVLVVIAGALAWLGFTSRTRGAARH
                     G"
     gene            complement(3529..>4274)
                     /locus_tag="PKV30_04125"
     CDS             complement(3529..>4274)
                     /locus_tag="PKV30_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HRL29441.1"
                     /translation="GRRSRRAAWVVLGLGGQAMVWANCSGGGYPHLPEGPFVDAGHGL
                     VRHVPSQTVWKRCAEGQRWVRNSCVGSAMEVTATAAADRVTAVNASARGTQNAGQTSW
                     RLPRLHELRWLVEAGCLLPAINQTQFPRTPAAPFWTETPFLGASNSAWTVDFAGGNAL
                     GTHRQAKAALRLVRVSANEEVLPRALALASPPRAPARQRQPVVPVGSVRPAAPTPISD
                     VVTINRPLVPVTPPPINFFPPPPSGPHCR"
     assembly_gap    4275..4374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4617..5504)
                     /locus_tag="PKV30_04130"
     CDS             complement(4617..5504)
                     /locus_tag="PKV30_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRL29442.1"
                     /translation="MANHNTDSTLSSFVSTQGESLALHDWPLSAGWPIRGVVLLVHGL
                     GEHVGRYVHVADQLNQWGFSVRGYDQYGHGESAGLRGELPTDDKLLDDLATVIDDTRA
                     RMDDRLPLILLGHSMGGLVAAQLVSLKLRRVDALVLSSPAFDLGLSVMQKALMNSLYR
                     IAPGARVGNGVEPRYLSHDLAMVDAYRADPLVHDRISARLAHFIDSAGAAVLKRAPRW
                     KVPTLLLYAGQDMVVRPAGSHAFASAAPPEWVTAQAYPMHYHELFNELDSAPVFEVLR
                     EWLVQRFPPVALEHAGELP"
     gene            complement(5671..7146)
                     /locus_tag="PKV30_04135"
     CDS             complement(5671..7146)
                     /locus_tag="PKV30_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRL29443.1"
                     /translation="MNAPLHREMAGAPAWDAAAALDQITRQWDGDIVRRLTEYIAVPA
                     KSLAFDPDWAAHGFIDHVVRDAADWVLAQKVPGLKLEVVRIDGRTPVIFFEIDGTKAG
                     AAQTVLMYGHLDKQPEFTGWRSDLGPWTPKYEDGKLYGRGGADDGYAVYAAIAAVQAL
                     KSQNVPHPRIVGLIETSEESGSPDLLPYIDALRPRLGDVGLVVCLDSGAGNYDQLWLT
                     TSLRGMAAGTLKVEVLTEGVHSGDASGLVPSSFRILRHVLDRLEDSATGRLLPASFHC
                     DIPAERVEQAKATAAILGDDLFKRYPWVHYDCGGESRRVLPMTGDPVDALLARTWRPT
                     LSVTGAEGFPAPQDAGNVLRPYTAFKLSLRLPPLVDAAQAVQDVKRLLEDNAPYQAKV
                     TFEHSGGATGWNAPASAAWFTQALDAASNAHFGAPCGYIGQGGTIPLMNQLSEGFPTS
                     QMMVCGVLGPRSNAHGPNEFLHVPYAKKLTAAVAEVIARME"
     gene            7253..7957
                     /locus_tag="PKV30_04140"
     CDS             7253..7957
                     /locus_tag="PKV30_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRL29444.1"
                     /translation="MTAALDTFPITRKWPARHPDRLQLYSLATPNGVKASIMLEETGL
                     PYEAHRVDFGSNDQTSPEFLSLNPNNKIPAILDPNGPGGQPLALFESGAILIYLAEKT
                     GQLLPQEPAQRYHALQWLMFQMGGVGPMFGQLGFFHKFAGKEYEDKRPRDRYVAESAR
                     LLGVLNGHLKGRAWMVGDDYSIADIAIFPWVRNLIGFYEARDLVQFGRFAEVQRVLDA
                     FVARPAVQRGLQVPAA"
     gene            8025..8837
                     /locus_tag="PKV30_04145"
     CDS             8025..8837
                     /locus_tag="PKV30_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRL29445.1"
                     /translation="MSNDTPRIRVGIGGWNFAEWRGGVFYPAGLPAAQELHFASRQLT
                     SIEINATFYATQKPATYARWRDETPDGFQFAIKAHRFATQRKALSDGAESVVHFLESG
                     VAELREKLGPIVWQLPPYRRFDADDLECFFALLPDKLDGLPLRHVLEPRNESFACADY
                     VRLARDYGVATVYTDSPDYPKMADVTGHFVYARLMRAEADEPTGYPPAELDAWAKRAR
                     AWSRGESPADLPLIESSAAAEAPSDVYVYFIAAAKARNPAAAMGLIERLPPG"
     regulatory      8923..9082
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9207..9731
                     /locus_tag="PKV30_04150"
     CDS             9207..9731
                     /locus_tag="PKV30_04150"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRL29446.1"
                     /translation="MNQTHASQFIAARAHPSSASGPFHSDSQPRIAFVRAGWHADIVG
                     QAERGFAAEYGRLWASAPPFIDLHEVPGAFEIPLLAQALAQRGNVQAIVACALVVDGG
                     IYRHDFVAHAVIDGLMRVQLDTGVPVFSVVLTPKDFHEHATHRDFFTQHFVAKGAEAA
                     RAVAQTLAAHAALA"
     gene            complement(9761..10282)
                     /locus_tag="PKV30_04155"
     CDS             complement(9761..10282)
                     /locus_tag="PKV30_04155"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRL29447.1"
                     /translation="MADIDHQPRHDLAGAEAGQKIKDIAGDARMCMFSTDVTQFPGDT
                     RPMAIQEVDDDGTVWFISSSESEKNRDIARDPRVVLTFQNDDKSSYLSLSGKASVHTD
                     KATIDKYWTAFAKAWFDGKDDPRVTVISVKPERGHYWETKSGKVIAMAKMTLTAITGS
                     KMDDGGVDGELTL"
BASE COUNT         1578 a         3475 c         3533 g         1745 t
ORIGIN      
        1 gcgctcgccg gcggcaccgg acattccgac actgtctgaa gccggcctgc cgggcttcga
       61 ggccgtgtcg tggtttgcgc tgtttgccgc gccgggcacg cccaaggcgg tggccgacaa
      121 gctgtcggtg caggtggccg aggtgttgaa gcagcccgac gtggcgaaga agctgtctga
      181 cctggggctg gagcccgtgg gatcgaggcc cgacgagctg gcgcgctacc agcagaccga
      241 gatcgccaag tgggccaagg tggtgaagga atcgggcgcg accgtggaat aaggcgctat
      301 cgccggggcg gcgttcaacg ccgcagtacg ttccacacca ggtcgacgcc ggccgaccac
      361 aggtcgtcga cggtgaagcc gctggcgtcg gcgtttgacc agcgctcctt ctgctcttcg
      421 cgcttggcgc gctcgcggtc gcgctccagg gcgaccagcg cgtcggccag gtcggtgggc
      481 ttggggcctt cgtcggcgcg gcgcgcctgc tggccgtaac cggccagcgc acggtccacc
      541 gcctgcggat cgagcagtga gaacgccgcg cggcaatacg ggcaggcgtc gtgctggcgc
      601 agatcgaccg gggcgccgca gctgccgcaa tggatgatgc gcacgcgctc ggccaggtcg
      661 gcgatttcgg gcttggtgag ctggcgcacg aagccctttt cgatcatgaa cgacgaaaag
      721 gtcgagaagc gcccatgccg ctgcgggcag cgataggtga tgtagcggcc ggtgcgcacc
      781 acgtcagcgc cctgcaccag accgcgcttg cagcgcgggc aattcaggtc gcgggacagc
      841 gggccttggg catcgtcgcg atgcgcgtgc aggtcgcgga acagatcgac cacgccttgc
      901 ggcgccatgc gcaggttctc gttgccatcg aaccagatgc cgtggcaggc gtggcacaag
      961 tccagctcca gcgtggcgcc gtggtgcgcg gccacggggt gcgcctgcat cggcgcgcga
     1021 caggaagggc aagtggtcgg ggcggcggcc aggcggtcgg tcatggtgcg gcgatggtag
     1081 cggcagccgg gcgggcgccg ctaccatcgg gcgtcacatt gcatccgcca tgccccgctt
     1141 tcacgttgcc ctgccgctgt ccaccgacgc cgaactggcg ctgccgcccg gcgctgcgcg
     1201 ccacgtgcag gtactgcggc tgcagccggg cggcgcgatc acggtgtttg atggcacggg
     1261 tggcgagtgg gatgcgacca tcacccgcat ggggcgctca gacgtgcagg tgcgcgtggg
     1321 cgcgcaccac gccgtcgagc gcgaatcaac ccgcgccgtg cacctggttg tgggcctgat
     1381 ggcggccgag cgcatggact ggctggtgga gaaagcgacc gagttgggcg ccgccagcct
     1441 gacgcccgtg ctgatggcgc gcagcagcct gcgcctgtcg ggcgagcggg ccgagaaaaa
     1501 gcgtgcgcac tggcaagccg tgtccaacgc ggcttgtgaa caaagtggcc gcaaccgctt
     1561 gctggacgtg cgcgaacagc tcacttttca ggagtattct cggaatgcaa cgaatgcgac
     1621 gccggcgcgc tgggtgctgt cactggcgcc cgatgcgcgc ccggtggccg gttgcgccgc
     1681 tgcgttgtcg ccaacagacg acgtgacgat ggtgtccggc cccgaaggcg gcctgagctc
     1741 cgaagaagac gccgctgccc gcgccgccgg ctggcagccg atcacgctcg gcgcgcgcgt
     1801 gctgcgggcc gaaacggcgc cgctggcggt gctggcgcgg ctcacgctct gacggcgcgc
     1861 cgccggttgc gctactggcg gatgaaatcg atcatcacgc cttcgggcgc acgctggcgg
     1921 tactcgatgc gcacgccgtc gccatagcgc tgctgcatct gcgccaggag cggcagcgga
     1981 tcgtggtcgt tgacgaagcg catcgcctcg cccggctgca gcgcatcgag cgcgccaaag
     2041 atcgccgcgt ggcgaaaccg cttggcgacg ccgcgcgcgt caaagccgta gatcgcctcg
     2101 ggcgtcgaat ggtcgtgcgg ctgcaggtct tgcagcggtt gaatcagggc cacggaaggg
     2161 aactcctcaa gcggattcgg tgatggaaag acgtgtggac tttgcgccaa cggcgcgggc
     2221 agttcaagtc gagcccttac gcggcatgct ggctttcaac tgccgggccg cgcatggtct
     2281 tgacgcagtt cagacgccca aggccggccg ccggatcggc ctacactgcc gcatgaaccg
     2341 cgccctgctg ctgctgacgc tgtgtcaggg ccttttcctg accaacaacg tcaccttcat
     2401 cgccatcaac ggcctggtcg gcctgcaact ggcgcccttc ggctggatgg cgacgctgcc
     2461 ggtgatgggc tacgtggttg gcggcgcgct ggccacgccg ctggtggcgc gcacgcaggc
     2521 gcgcttcggg cgcaagggct cgttccagat cgggctggtg gtggctctgg gctcggcgct
     2581 gctggccgcc tttgccgcct ggacgcgcca gttctggctg ctggtggcgg ccaccttgat
     2641 tgcgggctac tacagtgcca acggccagct ctaccgcttt gctgcggccg agctggcgcc
     2701 gccggcgtgg cgcgagaaag cggtgtcgct ggtgctggcc ggcggcttgc tgggcgccat
     2761 cgccggaccc aatctggcag cgtggacgcg caccgtgctg cctgtgccct tcgtcggcgc
     2821 ttatctggcg ctggcgctgg tggccttgct cgccatgggc gtgatggctt tcattgcgtt
     2881 tccgccgacg ccgcccaagg acgccggcag cgcggccggc cggccactgg cggagatcat
     2941 gcgccagccc gtgttcatgg tcgctaccgt gggcgcggcg ctgggctatg gcgtgatgaa
     3001 cctgctgatg gctgccacgc cgctggccat gcaggtgtgc ggcttcgact ttggggccgc
     3061 cgcgcaggtg ctgcagtggc acgtgatcgg catgttcgcg ccgggctttt tcaccgggca
     3121 tttgatcaag cgctttggca cgctgccgat catgggcgtg ggtgtgctgc tgaatggcgt
     3181 gtgcgtggcg atcgcgctgt cgggcgtcga tctgcagcag tttctggtgg ccttgttcgc
     3241 actcggcgtg ggctggaatt tcctcttcac cggcagcacc acactggcgt tgaccgccta
     3301 ccggccagaa gaaaaagacc gcgcgcaggc ggccatcaac ttctgcgtgt tcgccaccat
     3361 ggcgttcacc tcgttcgctt ctggcgcgct ggtgacgacg cagggctggc gctggttgaa
     3421 cctgggttcg ctgccggtgt tggtggtgat tgcgggcgcg ttggcgtggc tggggttcac
     3481 gtcccgtacc cgcggggccg cacggcacgg gtgattgcgt ttcgcggctt accggcagtg
     3541 cggaccggac ggtggcggcg gaaagaagtt gatgggcggc ggggtgaccg gcaccagtgg
     3601 ccggttgatg gtgacgacgt cggagatggg tgtcggcgct gcgggacgca cagacccgac
     3661 aggtacaacc ggctgtcgtt gacgcgctgg cgcccgcggc ggcgacgcca atgccagcgc
     3721 gcgcggcagc acctcctcgt tggcggaaac gcgtaccagc cgcaacgcgg ccttcgcctg
     3781 tcggtgggtg cccagcgcgt tgccgccagc gaaatcaacc gtccatgcgg agttcgacgc
     3841 acccaggaac ggcgtctcgg tccagaacgg cgcagccggg gtgcgcggaa actgggtctg
     3901 gttgatggcc ggcaacagac agccggcctc gacgagccag cgcaactcat gcaaacgcgg
     3961 caggcgccag ctggtctggc cagcgttctg tgtgcctcgg gcgctggcgt tgacggcggt
     4021 gacgcgatcg gcagccgccg tcgcggtcac ctccatggcg ctgccaacgc agctgttgcg
     4081 cacccagcgt tggccttcag cgcaacgctt ccaaacggtc tggctgggta cgtgacgcac
     4141 caaaccatgg ccggcgtcga caaacgggcc ttccggcaga tgcgggtaac cgccgccgct
     4201 gcaattggcc cacaccatgg cctgcccacc caggccgagc accacccaag ctgcccggcg
     4261 tgagcgacgg ccccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcctgc
     4381 ccacccaggc cgagcaccac ccaagctgcc cggcgtgagc gacggcccca tttctgactt
     4441 atcaaggtgc tcatcgtgcc ctcctttgcc aagccctccc ttgcagagcg acctggcgtg
     4501 cacgcgctgc tgcctttctt gagcgcagca ctgtttcagc accaatgtaa ttctttgtta
     4561 gttatggggg tatcccgtta acggggtaac ctgttcgatg cccgcccgcc acggcatcag
     4621 ggcaactcgc ccgcgtgctc cagcgccacc ggcggaaagc gttgcaccag ccattcgcgc
     4681 agcacctcga acaccggcgc gctgtccagc tcattgaaca gctcgtggta gtgcatggga
     4741 tatgcctgcg cggtcaccca ctcgggcggc gcggcgctgg caaaggcgtg gctgccggcg
     4801 ggtctgacga ccatgtcctg cccggcgtag agcagcagcg tgggcacctt ccagcgcggc
     4861 gcgcgcttga gcaccgcggc acccgcgctg tcgatgaagt gcgccagccg tgcactgatg
     4921 cggtcatgca ccagcgggtc ggcgcggtag gcgtcgacca tggccaggtc gtgcgacagg
     4981 tagcgcggct ccacgccgtt gcccacccgc gcccccggcg caatgcggta gagcgagttc
     5041 atcaacgcct tctgcatcac gctcaagccc agatcaaacg ctggcgacga cagcaccagt
     5101 gcatccacgc ggcgcagctt gagcgacacc agttgcgccg ccaccaggcc gcccatcgaa
     5161 tggccgagca ggatcagcgg caggcgatcg tccatgcgcg cgcgcgtgtc gtcgatgacg
     5221 gtggccaggt cgtccagcag cttgtcgtcg gtcggcagct cgccgcgcag gccggccgat
     5281 tcgccgtggc cgtattggtc gtagccgcgc accgaaaagc cccactggtt cagttggtcg
     5341 gccacgtgca catagcggcc cacgtgctcg cccaggccgt gcaccagcaa caccacgcca
     5401 cggatcggcc agcccgccga cagcggccag tcgtgcagcg ccagcgattc gccttgcgtg
     5461 ctgacgaaac tggacaacgt ggaatcggtg ttgtggttgg ccatattcgt tatccacggc
     5521 gctcgcgcgc cacccgcaac cgatgaaacc ctaagagcaa ctggccgcgg agcaggcctt
     5581 gcgtgggcgc cgcgcagcgg gcttggcccg gccgctggcg tcgtccctct tccagaggga
     5641 aggcggctgc aagccggctc agggggtgcg tcattccatg cgcgcgatga cttcagcaac
     5701 cgccgccgtc agcttcttgg cgtagggcac atgcaggaac tcgttcggcc cgtgcgcatt
     5761 gctgcgcggg cccagcacgc cgcagaccat catctgcgag gtcggaaagc cttcgctcaa
     5821 ctgattcatc agcggaatgg tgccgccctg gccgatgtag ccgcacggcg cgccgaaatg
     5881 cgcgttggac gcggcatcca gcgcctgcgt gaaccaggcg gccgatgcgg gtgcgttcca
     5941 gccagtggcg ccacccgaat gctcgaacgt caccttcgcc tgatagggcg cgttgtcttc
     6001 cagcaggcgc ttcacgtcct gcaccgcctg cgcggcatcg accagcggcg gcaggcgcag
     6061 gctgagcttg aaggcggtgt agggccgcag cacgttgccc gcatcctgcg gcgccggaaa
     6121 cccctcggcc cccgtcacgc tcagcgtcgg ccgccaggtg cgcgccagca gcgcgtccac
     6181 cgggtcgccc gtcatcggca gcacgcggcg tgattcgccg ccgcagtcgt aatggaccca
     6241 cgggtagcgc ttgaacaagt cgtcgcccag gatggcggcc gtcgccttgg cctgctcaac
     6301 gcgctcggcc ggaatgtcgc aatgaaagct ggctggcagc agccgcccgg tggccgagtc
     6361 ctccagccgg tcgagcacgt ggcgcaggat gcggaagctg ctcggcacca ggcccgacgc
     6421 atcgccggag tgcacaccct cggtcaacac ctcgaccttc agcgtgccag ccgccatgcc
     6481 gcgcaggctg gtggtgagcc acagctggtc gtaattgccg gcgcccgaat cgaggcacac
     6541 gaccagcccc acgtcgccca gccgcgggcg cagcgcgtcg atgtagggca gcaaatcggg
     6601 cgagccgctt tcctcgctgg tctcgatcag gccgacgatg cgcgggtgcg gcacgttctg
     6661 gcttttgagc gcctgcacgg cggcgatggc cgcgtacacc gcgtagccgt cgtcggcgcc
     6721 gccgcggccg tagagcttgc cgtcttcgta cttcggcgtc cacggcccca ggtcgctgcg
     6781 ccagccggtg aactccggct gtttgtcgag gtggccgtac atcagcaccg tttgcgcggc
     6841 gccggctttc gtgccatcga tctcgaagaa gatcaccggc gtgcggccat cgatgcgcac
     6901 cacctccagc ttcaagcccg gcaccttctg cgccagcacc cagtcggccg catcgcgcac
     6961 cacgtggtcg atgaagccgt gcgcggccca gtcggggtcg aaggcgagcg acttggccgg
     7021 cacggcgatg tactccgtca ggcggcgcac gatgtcgccg tcccactggc gggtgatctg
     7081 gtcgagcgcg gcggcggcgt cccacgcggg ggcgccggcc atttcacggt gcaggggtgc
     7141 gttcatggca atgggcttgt cagcaaagcg aaagaaaacg cgactttacg cccttgcgcc
     7201 gccccatgcc accacctgcg ccacgggcat cacggcggcg tatattggcc gcatgaccgc
     7261 cgcactcgac accttcccca tcacccgcaa atggccggcg cgccacccgg atcggctgca
     7321 gctgtactcg ctggccacgc ccaacggcgt caaggcgtcg atcatgctgg aggaaaccgg
     7381 cttgccctac gaagcgcacc gcgtggactt tggcagcaac gaccagacct cgcccgaatt
     7441 cctcagcctc aacccaaaca acaagatccc cgccatcctt gacccgaacg gcccgggcgg
     7501 tcaaccgctg gcgctgttcg agtcgggagc catactgatc tacctggcgg agaagaccgg
     7561 ccagctgctg ccccaagaac cggcccagcg ctaccacgcg ctgcagtggc tgatgttcca
     7621 gatgggcggc gtcgggccca tgtttggcca gctcggcttc ttccacaaat tcgccggcaa
     7681 ggagtatgag gacaagcgcc cgcgcgaccg ctacgtggct gagagcgcgc gcctgctcgg
     7741 cgtactgaac gggcacctca aaggccgcgc ctggatggtg ggcgatgact acagcatcgc
     7801 cgacatcgcc atcttcccgt gggtgcgcaa cctgatcggc ttttacgaag cgcgcgatct
     7861 ggtgcagttc ggccgctttg ccgaagtgca gcgcgtgctg gatgcgtttg tggcgcggcc
     7921 tgcggtgcag cgagggctgc aggtgccggc cgcgtgaatc tgaatcaaat caggctccag
     7981 cgcttgtgta tcaagcgcga gaagctattg attgagtagc aaacatgtcg aatgacacgc
     8041 cccgcatccg cgtcggcatc ggcggctgga acttcgccga atggcgcggc ggtgtgttct
     8101 acccggccgg tttgcccgcc gcgcaagagc tgcacttcgc cagccggcag ctgaccagca
     8161 tcgagatcaa cgccaccttc tacgccacgc aaaagccggc cacctacgcg cgctggcgcg
     8221 atgagacgcc ggacggcttt cagttcgcca tcaaggcgca ccgctttgcc actcagcgca
     8281 aggccttgag cgacggcgcc gagagcgtgg tgcattttct ggaaagcggt gttgccgaac
     8341 tgcgcgaaaa gcttggcccc atcgtgtggc agctgccgcc gtatcgccgg ttcgatgccg
     8401 atgacttgga atgcttcttc gccctgctgc ccgacaagct cgatggcctg ccgctgcgcc
     8461 atgtgctgga gccacgcaac gagagctttg cctgcgccga ctatgtgcga ctggcgcgcg
     8521 actacggcgt ggccacggtc tacaccgatt cgcccgacta cccgaaaatg gccgacgtga
     8581 cgggccattt cgtctacgcg cggctgatgc gggccgaggc ggacgagccc accggctacc
     8641 cgcccgccga actggacgcc tgggcaaagc gtgcccgagc ttggtcgcgc ggcgaatcgc
     8701 ccgccgattt gcccttgatt gagtcctccg ctgccgccga agcaccgagc gatgtctacg
     8761 tctatttcat cgccgccgcc aaggcccgca acccagcggc ggcgatgggt ctgatcgaac
     8821 ggctgccgcc gggctgagcg gcgctgtcgc ccgcgcatct gggcggcgac ggggtcattc
     8881 gtatcatgtc agcgcgacgg cgctgtgaag gcgccgtcgc acaagcgttc tcagggcggg
     8941 gtgaaagtcc ccaccggcgg tatctgcttc agcgatggag cagcagcccg cgagcgctcc
     9001 ggcggctgat gtctgccgga ggtcagcaga cctggtgcga tgccagggcc gacggtcaca
     9061 gtccggatga aagagagcgt gcgaagcgtt gccgcttggc tgcggtcgcg gcgtgccggg
     9121 ctttgacgcc cagcgcaccg ctggcgtggc cgcggcgcgt ttcgcgtgcg ccctgattct
     9181 ttctcacgtt gttcacggag atgtcgatga atcagaccca tgcttctcaa ttcatagctg
     9241 ctcgcgctca cccatcaagc gccagcggcc cttttcattc cgattcgcag ccccgcatcg
     9301 ccttcgtgcg ggccggctgg cacgccgaca tcgtcggcca ggctgagcgg ggcttcgcgg
     9361 ccgaatacgg acgcctgtgg gccagcgcgc cgcccttcat cgatttgcac gaagtgcccg
     9421 gcgctttcga gattccgctg ctcgcgcaag ccctcgccca acgcggcaac gtgcaggcca
     9481 tcgtggcttg cgcgctggtg gtcgatggcg gcatttaccg ccacgatttc gtggcccacg
     9541 ccgtcatcga tggcctgatg cgcgtgcaac tcgacaccgg cgtgccggtg ttctcggtgg
     9601 tgctgacccc caaggacttt cacgaacacg ccacgcaccg cgacttcttc acccagcact
     9661 tcgtcgccaa gggcgccgaa gccgcgcgcg cggtggcgca gacgctggcg gcgcatgcgg
     9721 cgctggccta gcgaggcggg cgcgttgtcg cgccccgccc tcaaagcgtc aattcgccat
     9781 ccaccccacc atcgtccatc ttgctgccgg tgatggccgt gagcgtcatc ttggccatgg
     9841 cgatcacctt gccggacttg gtttcccagt agtggccgcg ctcgggcttg acgctgatga
     9901 ccgtaacgcg cgggtcgtcc ttgccgtcga accaggcttt ggcaaaggct gtccagtatt
     9961 tgtcgatggt ggccttgtcg gtgtgaacgc tggccttgcc cgataggctc aggtagctcg
    10021 acttgtcgtc gttctggaag gtgagcacga cgcgcgggtc gcgcgcgatg tcgcggttct
    10081 tctcgctctc gctggagctg atgaaccaga cggtgccatc gtcgtccact tcctgaatcg
    10141 ccatcggccg cgtgtcgcca gggaactgcg tcacgtcggt cgagaacatg cacatgcgcg
    10201 cgtcgcccgc gatgtccttg atcttctggc cggcttcggc gccggcgagg tcgtgtcggg
    10261 gttgatggtc gatgtcggcc atgcggatct cctgatggtg ttcaaaagga gcatcttggc
    10321 cgaccacgcg gcgccgcgct gtcggacagc ggcgcggcgc ggcgcgccgt gggcaggcgc
    10381 gacgcgtgac caccggcgcc gttattcggg cacgccggct agaaacttct c
//
LOCUS       DAOMVO010000051        10424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1547_length_10424_cov_13.750506, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000051 DAOMVO010000000
VERSION     DAOMVO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_10424_cov_13.750506"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..698)
                     /locus_tag="PKV30_04160"
     CDS             complement(57..698)
                     /locus_tag="PKV30_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRL29448.1"
                     /translation="MAWTQDQMAARAAQELEDGFYVNLGIGIPTLVANHVPANKEVWL
                     QSENGMLGIGPFPTEAEVDADLINAGKQTVTTIKGSSIFGSHDSFAMIRGGKINLSIL
                     GAMQVTDKGDLANWMIPGKMVKGMGGAMDLVAGVARVIVLMEHVAKKKDGSTDMKILP
                     QCTLPLTGVGVVDRIITDLGVFDVTDKGLKVAELADGVTLEEAQSKTGVPLLQ"
     gene            complement(711..1412)
                     /locus_tag="PKV30_04165"
     CDS             complement(711..1412)
                     /locus_tag="PKV30_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRL29449.1"
                     /translation="MNKIYPSADEALSGVVKDGQLLAVGGFGLCGIPEALIQALHDSG
                     VKNLTAISNNAGVDGFGLGKLLETRQIKKMIASYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTLVAEGKELREFDGETYVMERSLVADVALVKADVA
                     DKSGNLRFHLTARNFNPACAMSGKVCIVEVERIVETGELAPDDIHLPGIYVHRIVLNA
                     NPEKRIEKRTLRETK"
     gene            1493..2062
                     /locus_tag="PKV30_04170"
     CDS             1493..2062
                     /locus_tag="PKV30_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRL29450.1"
                     /translation="MDIPAAPDYRLAFQLAPVGLILSRERRIVDCNERVCEIFGATPE
                     QLVGQSFRMLYPSADEYERIGARMAPILNAHGVYADNRVMRRVGGARQGEAFWCHVTG
                     RAIDRAAPHAAGIWTFEDLSAERPVTAALTAREREVAAQLMQGLTSKEIGRALGISHR
                     TVEIYRARLMRKYRASTAAELVQKLMSGR"
     gene            complement(2158..2811)
                     /locus_tag="PKV30_04175"
     CDS             complement(2158..2811)
                     /locus_tag="PKV30_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HRL29451.1"
                     /translation="MNPAQSFPTDIYTEPVADVHTLANLGPLTRMAGVWRGTQGMDRK
                     PKAGGPKEQAYVGRIELQPIDPQTNGPQLFYGLRYHTHITKPGQVKTYHDQVGYWLWE
                     PATGTVVQTLTIPRGQTAMAGGTAAADADEFEVVATLGSATYGICSNPFLDHAFKTTE
                     FRIKVGFNPDGSWWYDETTTMQIEGQAAPFQHTDHNVLTRIAEPTPNPLALAAAQGQ"
     gene            complement(2907..3293)
                     /gene="pcaC"
                     /locus_tag="PKV30_04180"
     CDS             complement(2907..3293)
                     /gene="pcaC"
                     /locus_tag="PKV30_04180"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HRL29452.1"
                     /translation="MNLPDDPDFKAGLATRRQVMGDDFVDRALAGATAFTEPMQHYIT
                     RNAWGDVWQRPGLDMKTRSLITMSMLIALGRQHELKGHVRGALNNGVTPEQIQEVLLH
                     ASIYCGVPCAVEAFRSAAEVVDGPKA"
     gene            3354..3950
                     /locus_tag="PKV30_04185"
     CDS             3354..3950
                     /locus_tag="PKV30_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854445.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRL29453.1"
                     /translation="MNRYPLADINTLPDDIKTKVLEVQAKAGFVPNVFLGLARRPAEW
                     RAFFAYHDALMEPESVGRSSNLTKGDREMIVTTTSGANQCLYCVVAHGALLRIYEKKP
                     LVADQVAVNYRKATDITPRQKAMLDFAMKVCERSHEINDDDFAPLHAHGFDDEDIWDI
                     AAITAFFGLSNRIASFSGMQPNPEFYLMGRVPKEKKPA"
     gene            3984..4625
                     /locus_tag="PKV30_04190"
     CDS             3984..4625
                     /locus_tag="PKV30_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRL29454.1"
                     /translation="MSEKEEVARSVEVSVAFHGERCIHSRNCVIDRPDVFVPNVKGEW
                     IHPDRATADEVLEIAHNCPSGAIQCSRSDGSALEQAPVVNTVRVRENGPLAFRGELTV
                     AGEAKGMRATLCRCGLSQRKPYCDSSHVAAGFEATGEPATEPSEALATRNGPLNVAPQ
                     RNGPLHVTGNVEIISGTGRTVDRVTDAYLCRCGHSSHKPFCDGTHAKIGFQAP"
     gene            4741..5988
                     /gene="lplT"
                     /locus_tag="PKV30_04195"
     CDS             4741..5988
                     /gene="lplT"
                     /locus_tag="PKV30_04195"
                     /inference="COORDINATES: protein motif:HMM:NF008397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HRL29455.1"
                     /translation="MTRGIACLIAAQFVSGLADNALLIVAMARLAEMGRSAWLAPLLK
                     AVFTLAYVLLAPWVGALADRWPKARVMLAANGLKALACALMALAIEPLLAFALAGVGA
                     AAYLPAKYGLVTELSPPEQLVHANGWLEVTTVGAIILGTALGGWLVGPWLLAHGARHM
                     GHALPVDSPLGAAILLVLALYALAAALNLLIPSSGARPHAMQPSFSALLTRFAHAQRT
                     LWRDAQGGLSLGVTTLFWGAGATLQFVVLAWAQDNLSLSLSHAAYLQGVVAIGIGVGA
                     LAAGRWVRLHQAPRVLQLGVLMGACVPLLTLVHSVSVAAPLLAAVGALAGFFVVPMNA
                     LLQHRGQQLLGAGESIAVQNFNENLSVLAMLGAYAALQMTGWPLHRTVFALGSVVALG
                     VLSVMWRYRLVGARRQLLEGEQT"
     gene            5985..7043
                     /locus_tag="PKV30_04200"
     CDS             5985..7043
                     /locus_tag="PKV30_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRL29456.1"
                     /translation="MKIMIVTDAWEPQVNGVVRTLKMTTRHLQEMGHSTHILSPLDFK
                     SMPCPTYPEISLALATKGAVARHIDKVAPDCLHIATEGPLGWAARGVALQRGWPFTTA
                     YHSRFPEYVQARVRVPLGWSYALLRKFHNAGRATLAPTPAIVDDLKARGFTQARLWSR
                     GVNLEAFTPDGPRLERTRSPVFLYVGRLAVEKQVHKFLELDLPGEKWVAGAGPEEARL
                     KAQYPQARWFGVLSGDELATVYRSADVMVFPSVTDTFGLVMAESMACGTPVAAYPVPG
                     PIDVVGHDSPGGAIDNDLRAACMAALKKPRDGVRRHSEQFNWPAATRQFERALVPLRA
                     GAAASNAHAEAHAALQNK"
     gene            complement(7092..7514)
                     /locus_tag="PKV30_04205"
     CDS             complement(7092..7514)
                     /locus_tag="PKV30_04205"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL29457.1"
                     /translation="MKIDIPDQKKLVFETHIAVRWGDMDAMGHVNNATYFRYMETARI
                     DWFRSIGCVPDAQGEGPVIVNAFCNFYRQFEYPDEVLLKMYVSDPGRTTFESWATMEK
                     VAEPGVVCAAGGATTIWVNFPQQKAVGLPDWIRAIVSD"
     gene            complement(7511..8428)
                     /locus_tag="PKV30_04210"
     CDS             complement(7511..8428)
                     /locus_tag="PKV30_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL29458.1"
                     /translation="MLTGILAGLLAGALWGLVFVAPRMVSGFAGIDLTAGRFVVYGVV
                     SVAVLAVSWRRARKPTWPQAWGALWLSVLGFTGYYWILVLAIRDAGTEMPALIVGTIP
                     IWVMLLGKPQGLRWVALLPGLLLTLAGLYFMAKGTLAHADQAPAALNTIASQSAYWRG
                     IGLAVAAMACWTAFALLNAAWLRRNPDVGATDWANWLGVATGVGGLLLTLTLGTPLPQ
                     LVAQPGWPLFALIVVAIGLGSSWLATILWNIASQRLSASLCGQLIVSETLFALLYSFM
                     WDKRWPTITQWTAAALFTAGILASIKAHR"
     gene            complement(8561..9343)
                     /locus_tag="PKV30_04215"
     CDS             complement(8561..9343)
                     /locus_tag="PKV30_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL29459.1"
                     /translation="MSYSIDLSGRVAFVTGASGGLGAEFARTLARAGAGVVLASRRIE
                     KLKDLRARIEGEGGDAHVVECDVTDVQSIKGAVAHAETEMGSIDILINNSGVSTTQRI
                     QDVTEDDYDFIFNTNVRGAFFVAQEVGKRMIARAKGAAPGSFTGGRIINIASMAGLKV
                     LPQIGVYCMSKAAVVQMTKAMALEWGRFGINVNAICPGYIDTEINHHHWQTEQGQKLI
                     SMLPRKRVGEPKDLDAVLMMLASDQSHFVNGAVIAADDGFAV"
     gene            9493..>10424
                     /locus_tag="PKV30_04220"
     CDS             9493..>10424
                     /locus_tag="PKV30_04220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL29460.1"
                     /translation="MTPEEIIQQYGPREAMEYDVVIVGAGPAGLSAAIRIKQLAAEKG
                     NDVSVVVLEKGSEPGAHILSGAVMDPKALSELIPDWKEKGAPLHQQVTDDAYVFLNEG
                     GGGARVPNLLLPPFANNHGNYIVSLGNVVRWLATQAEELGVEIFPGFPAAEVLYNEDG
                     SVKGVATGNMGVGKDGEPTENFQLGMELLGKYTIFAEGSRGNLGKQLIARYKLDAHSD
                     PQSYAIGIKELWEIDPSRHTPGFVMHTAGWPMDEHTYGGAFLYHAEDNKVYCGYVVGL
                     NYKNPYLSPFEEFQRWKTHPRVKWYFTDDAGNVNA"
BASE COUNT         1735 a         3448 c         3454 g         1787 t
ORIGIN      
        1 agaaaaacgg cccgaatcgc ttcgggccgt tggcagacaa gcgcgagacg ctctttttat
       61 tgaagcagcg gcacgcccgt cttggactgc gcttcttcca gcgtcacgcc gtcggccagc
      121 tcggccactt tcaggccctt gtccgtcacg tcgaacacac ccaggtcggt gatgatgcgg
      181 tccaccacgc ccacgccggt cagcggcagc gtgcactgcg gcaggatttt catgtcggtg
      241 ctgccgtctt tcttcttggc gacgtgctcc atcagcacga tgacgcgcgc cactccggcc
      301 accaggtcca tggcgccgcc catgcccttg accatcttgc cggggatcat ccagttggcc
      361 aggtcgccct tgtcggtgac ctgcatggca cccaggatgc tgaggttgat cttgccgccg
      421 cgaatcatgg cgaagctgtc gtggctgccg aagatgctgc tgcccttgat ggtggtcacg
      481 gtctgcttgc cggcgttgat gaggtcggcg tccacctcgg cctcggtcgg gaaggggccg
      541 atgccgagca tgccgttttc ggattgcagc cacacctctt tattggcggg cacgtggttg
      601 gccaccagcg tggggatgcc gatgccgagg ttgacgtaga aaccgtcttc cagctcttgc
      661 gcggcgcgcg ccgccatttg gtcttgggtc caggccatgt gcgtgctcct ttatttggtc
      721 tcgcgcagcg tgcgcttttc aatgcgtttt tcggggttgg cattcagcac gatgcggtgc
      781 acgtagatgc ccggcaggtg aatgtcgtca ggcgccagtt cgccggtttc gacgatgcgc
      841 tcgacctcga cgatgcacac cttgcccgac atggcgcagg ccgggttgaa gttgcgcgcc
      901 gtcaggtgaa agcgcaggtt gccgctcttg tcggccacgt cggctttcac cagcgccacg
      961 tcggccacca gcgagcgctc catcacgtag gtttcgccgt cgaattcgcg cagctccttg
     1021 ccctcggcca ccagcgtgcc gacaccggtc ttggtgaaga aggccggaat gccagcgccg
     1081 ccagcgcgca acttttcagc cagcgtgcct tgcggggtga attccagctc cagctcgccc
     1141 gccaggtact ggcgctcgaa ttccttgttc tcgcccacgt agctggcgat catcttcttg
     1201 atctggcgcg tttccagcag cttgcccagg ccaaagccgt caacgccggc gttgttgctg
     1261 atggcggtga ggttcttcac gcccgaatcg tgcagcgcct ggatcagcgc ctcggggatg
     1321 ccgcacaggc cgaagccgcc gacggcgagc agctggccgt ccttgacgac gccgctcaaa
     1381 gcctcgtcgg ccgagggata aatcttgttc acaggcaatg tctcctcaaa aaaatgggtg
     1441 ccacgggaca acccctaacc atactacgta gccgattacg tagtatttcg taatggacat
     1501 ccctgccgcg cccgattacc ggctggcgtt tcagctggct ccggtggggc tgatcctctc
     1561 gcgcgagcgg cgcatcgtgg actgcaacga gcgcgtgtgc gagatcttcg gcgccacgcc
     1621 tgagcagctc gtcggccaga gctttcgcat gctgtacccg agtgccgacg agtacgagcg
     1681 catcggcgcg cgcatggcgc cgatcctgaa cgcgcacggc gtgtatgccg acaaccgcgt
     1741 gatgcgccgc gtgggcggcg cgcgccaggg cgaggccttc tggtgccacg tgacgggccg
     1801 cgccatcgac cgcgcggcgc cgcacgcggc cggcatctgg acgtttgagg acctgagcgc
     1861 cgagcgcccg gtgacggccg cgctcaccgc gcgcgaacgc gaagtggcgg cgcaactgat
     1921 gcaggggctg accagcaagg agattggccg cgcgctgggc atcagccacc gcacggtgga
     1981 gatctaccgc gcgcggctga tgcgcaagta ccgcgccagc accgcggccg agctggtgca
     2041 gaagctgatg tcggggcgct gacgccggtg ccgtcaacta cacatttgat agctgcttgc
     2101 gcttgatcag caagcgctgg cggcgttttt tctgtgaaac acgccactgc gccgtgccta
     2161 ttggccctgt gccgcggcca acgccagcgg gttcggcgtt ggctcggcga tcctggtcag
     2221 cacgttgtgg tcggtgtgct gaaacggcgc ggcctggccc tcgatctgca tcgtggtggt
     2281 ctcgtcgtac caccagctgc catcggggtt gaagccgacc ttgatgcgga actcggtggt
     2341 cttgaaggcg tggtcgagaa acgggttgga gcagatgccg taagtagcgc tgcccaacgt
     2401 ggccaccacc tcgaactcgt ccgcgtcggc cgccgccgtg ccgccggcca tggccgtctg
     2461 gccgcgcgga atggtcagcg tctgaaccac cgtgcccgtg gccggctccc acagccagta
     2521 gccgacctgg tcgtggtagg tcttcacctg ccccggcttg gtgatgtgcg tgtggtagcg
     2581 caggccgtag aacagttgcg ggccattggt ctgcgggtcg atgggttgca gctcgatgcg
     2641 cccgacatag gcctgctcct tcgggccgcc cgccttgggt ttgcgatcca tgccctgcgt
     2701 gccccgccac acgcccgcca tgcgcgtgag cgggcccagg ttggccagcg tgtgcacgtc
     2761 ggccacgggc tcggtgtaga tgtcggtggg gaaagattgg gcaggattca cggtatcgcc
     2821 tccttgcggc gtgcacgtaa aactattgat ttgatagctg ccagcgcttg atggacaagc
     2881 gctggcgccc gaattgatcg ctaaattcag gccttcggcc cgtccaccac ctcggcggcg
     2941 ctgcggaacg cctccaccgc gcagggcacg ccgcagtaga tgctggcgtg cagcagcact
     3001 tcctgaatct gctcgggcgt cacgccgttg ttgagcgcgc cgcgcacgtg gcccttcaat
     3061 tcgtgctggc ggcccagcgc aatcagcatc gacatcgtga tgaggctgcg cgtcttcatg
     3121 tccagcccgg ggcgctgcca cacgtcgccc caggcgttgc gcgtgatgta gtgctgcatg
     3181 ggctcggtga aggcggtcgc gccggccagt gcgcggtcga caaagtcgtc gcccatcacc
     3241 tggcggcgcg tggccaggcc ggccttgaag tcggggtcgt cgggcaggtt catcgaaagc
     3301 tccttgaatc gcataatgga ccagaacacc ccaccgattc tgaaggagac cccatgaacc
     3361 gctacccgct tgccgacatc aacacgctgc cggacgacat caagaccaag gtgcttgaag
     3421 tgcaggcaaa ggccggcttc gtgcccaacg tgttcctggg cctggcgcgc cgaccggccg
     3481 aatggcgcgc cttcttcgcc taccacgacg cgctgatgga gcccgagagt gtgggccgca
     3541 gcagcaacct caccaaaggc gaccgcgaga tgatcgtcac caccaccagc ggcgccaacc
     3601 agtgcctgta ttgcgtggtg gcgcacggcg cgctcttgcg catctacgag aagaaaccgc
     3661 tggtggccga ccaggtggcc gtcaactacc gcaaggcgac cgacatcacg ccgcgccaga
     3721 aggccatgct cgactttgcg atgaaggtgt gcgagcgctc gcacgagatc aatgacgacg
     3781 acttcgcgcc gctgcacgcg cacggctttg atgatgaaga catctgggac atcgcggcca
     3841 tcaccgcgtt cttcggcctg tcgaaccgca ttgcgtcgtt cagcggcatg cagccgaatc
     3901 cggagttcta tctcatgggg cgggtgccca aggagaagaa gcccgcctga cgctttcgcg
     3961 ttgcccagcc ccggagattc atgatgagcg aaaaagaaga agtcgcccgc tccgtcgaag
     4021 tcagcgtcgc gttccacggc gagcgctgta tccactcgcg caactgcgtg atcgaccggc
     4081 ccgacgtctt cgtgcccaac gtcaagggcg agtggattca ccccgaccgc gccacggccg
     4141 atgaggtgct ggagatcgcg cacaactgcc cttccggcgc catccagtgc agccgatccg
     4201 atggcagcgc actggagcag gcgcccgtcg tgaacacggt gagggtgcgc gaaaacgggc
     4261 cactggcctt tcgcggcgaa ctcacggtgg cgggcgaagc gaagggcatg cgcgccacgc
     4321 tgtgccgctg cgggctgtcg caacgcaagc cgtactgcga ttccagccac gtcgcggccg
     4381 gcttcgaagc cacgggcgag ccggccaccg aaccctccga agcgctggcc acgcgcaatg
     4441 gcccgctcaa cgtggcgccg cagcgcaacg ggccgttgca cgtcaccggc aacgtcgaaa
     4501 tcatcagcgg caccggccgc acggtggatc gcgtgaccga cgcgtacctg tgccgttgcg
     4561 gccattccag ccacaagccg ttttgcgacg gcacgcacgc gaagatcggc ttccaggcgc
     4621 cttgatcggc gcatgtcgcg acgccgcaac caggccggcg gttgcccgcc gtttcgtcat
     4681 caagccgcca cacggccgca gttcaatcgt cacatgcaaa ggcgcctttt ctagcgttcg
     4741 atgacgcgcg gcatcgcctg cctgatcgcg gcacagttcg tgtcgggcct ggccgacaac
     4801 gccttgctca tcgtcgccat ggcgcggctg gccgaaatgg gccggagcgc ctggctggcg
     4861 ccgctgttga aggcggtgtt cactctggcg tatgtgctgc tggcgccctg ggtcggcgcg
     4921 ctggccgacc gctggcccaa ggcgcgggtg atgctggccg ccaacgggct gaaggcgctg
     4981 gcctgcgcgc tgatggcgct ggcgattgag ccgctgctgg cattcgcgct ggcaggcgtc
     5041 ggtgcggcgg cgtatttgcc ggccaagtat gggctggtga ccgagctgtc gccacctgaa
     5101 caactggtgc acgccaacgg ctggctggaa gtgaccaccg tcggcgccat catcctcggc
     5161 accgcgctgg gcggttggct ggtcggcccg tggctgctgg cacatggggc gcggcacatg
     5221 ggccacgcgc tgccggttga ctcaccgctg ggtgccgcga tcctgctggt gctggcgctg
     5281 tacgcgctgg cggccgcgct caacctgctg attccgtcca gcggcgcgcg gccgcatgcg
     5341 atgcagccgt cgttcagcgc gctgctgacg cgctttgccc acgcgcagcg cacgttgtgg
     5401 cgcgacgctc agggtggcct gtcgctgggc gtcaccacgc tgttctgggg cgccggcgcc
     5461 acgctgcaat tcgtggtgct ggcgtgggcg caggacaacc tgtcgttgag cctgtcacac
     5521 gcggcctacc tgcaaggcgt ggtggccatc ggcattggcg tcggcgcgct ggccgccggg
     5581 cgctgggtgc ggttgcacca ggcgccgcgc gtgctgcagc tgggcgtgct gatgggcgcc
     5641 tgcgtgccgt tgctgacgct ggtgcacagc gtcagcgtgg cggcgccgct gctggccgcc
     5701 gtgggcgcgc tggcgggctt tttcgtcgtg ccgatgaacg ccttgttgca gcaccgcggc
     5761 cagcaactgc tgggcgcggg cgaatcgatt gcggtgcaga acttcaacga gaacctgagc
     5821 gtgctggcca tgctgggcgc ctacgcggcg ctgcagatga cgggctggcc gctgcaccgc
     5881 accgtgttcg cgctgggcag cgtggtggcg ctgggggttt tgagtgtgat gtggcgctac
     5941 cgcttggtgg gcgcgcgcag gcagctattg gaaggagagc aaacgtgaaa atcatgatcg
     6001 tcaccgatgc gtgggagccg caggtcaacg gcgtggtgcg cacgctgaag atgaccacgc
     6061 gccacctgca ggagatgggg cacagcacgc acatcctgtc gccgctcgac ttcaagtcga
     6121 tgccctgccc gacgtacccc gaaatctcgc tggcgctggc caccaagggc gcggtggcgc
     6181 ggcacatcga caaggtggcg cccgactgcc tgcacatcgc caccgagggg ccgctcggct
     6241 gggccgcgcg cggcgtggcg ctgcagcgcg gctggccgtt caccacggcg taccacagcc
     6301 gttttcccga atacgtgcag gcgcgtgtgc gggttccgct gggctggagc tacgcgctgc
     6361 tgcgcaagtt tcacaacgcc ggacgcgcca cgctggcgcc cacgccggcc atcgtcgatg
     6421 acctgaaggc gcgcggcttc acgcaggcgc ggctgtggtc gcgcggggtg aatctggagg
     6481 ctttcacgcc cgacgggcca cggctggagc gcacccgcag cccggtgttc ctctacgtcg
     6541 gccggctggc ggtggaaaag caggtgcaca agtttctgga actggacttg cccggcgaaa
     6601 aatgggtcgc cggcgccggg ccggaagagg ccaggctgaa agcccagtat ccgcaggcgc
     6661 gctggttcgg cgtgctgagc ggcgacgaac tggccaccgt ctaccgcagc gccgacgtga
     6721 tggtcttccc cagcgtgacc gataccttcg gcctggtgat ggccgaatcg atggcttgcg
     6781 gcacgccggt ggccgcctac ccggtgcctg ggccgatcga cgtggtgggc cacgattcgc
     6841 ccggcggtgc catcgacaac gacctgcgcg ccgcctgcat ggccgctttg aagaagccgc
     6901 gcgacggcgt gcggcgccac tcggagcagt tcaactggcc agcggcaacg cgacagtttg
     6961 aacgggcgct ggtgccgctg cgcgcgggcg cggcagcgtc aaacgcgcat gccgaggcgc
     7021 acgccgcact acaaaacaaa tagctgcttg cgcttgtctt gcaagcgcca gcggcttttt
     7081 tctcttgaat atcagtcgct gacgatggcg cgaatccagt ccggcagccc caccgccttc
     7141 tgctgcggaa agttgaccca gatggtggtc gcgccgcccg ccgcgcacac cacgccgggc
     7201 tcggccacct tttccatcgt cgcccagctc tcgaaggtgg tgcggccggg gtcgctgacg
     7261 tacatcttca gcagcacctc gtccgggtac tcgaactggc gatagaagtt gcagaacgca
     7321 ttgacgatca ccggcccctc gccctgcgcg tcgggcacgc agccgatgga gcgaaaccag
     7381 tcgatgcgcg ccgtttccat gtagcggaag taggtggcgt tgttgacgtg gcccatggcg
     7441 tccatgtcgc cccagcgcac ggcgatgtgg gtttcgaaca ccagtttttt ttggtcggga
     7501 atgtcgatct tcatcggtgt gccttgatgg atgccaggat gccggcggtg aacaacgcag
     7561 ccgccgtcca ctgcgtgatc gtgggccagc gcttatccca catgaaagag tacagcagcg
     7621 cgaacagcgt ctcgctcacg atgagctggc cgcacaggct ggccgacagg cgctggctgg
     7681 cgatgttcca caagatggtg gccagccagc tggagcccaa accaatcgcc accacgatca
     7741 gcgcgaacag cggccagccg ggttgcgcga caagctgcgg cagcggcgtg ccgagcgtca
     7801 acgtcagcag caatccgccc actccggtgg ccacgccaag ccagttagcc cagtcggtgg
     7861 cgcccacgtc ggggttgcgg cgcagccagg ccgcattgag cagcgcaaac gccgtccagc
     7921 acgccatggc ggccacggcc agaccgatgc cgcgccagta ggcggattgg cttgctatgg
     7981 tgttgagagc tgccggcgct tgatcggcgt gcgccagcgt gccttttgcc atgaaataca
     8041 ggccggcgag cgtcaacagc agccccggca acagcgccac ccagcgcagt ccttgcggct
     8101 tgcccagcag catcacccag atcgggatgg tgcctacgat gagcgccggc atctcggttc
     8161 cggcgtcgcg gatggccagc accagaatcc agtagtagcc ggtaaagccc agcaccgaca
     8221 gccacagcgc gccccaggcc tgcggccacg tcggcttgcg cgcgcgccgc cacgacacgg
     8281 ccagcaccgc gaccgagacc acgccgtaga cgacgaagcg ccccgccgtc aggtcaatgc
     8341 ccgcaaagcc gctgaccatg cgcggtgcca cgaacaccag gccccacagc gcgccggcga
     8401 gcaggccggc taggatgccg gtcagcatgg agcgatgacg gaatgactgc gccgctgcgc
     8461 ggctgcaatg acgaaatgac gaggcaacgc aaagcgcgtg tcgcgggtca tggcggcgca
     8521 gccgccgtca tcaacgccgt gcagcggcga ggtcatttcg tcagacggca aacccatcgt
     8581 ccgccgcaat caccgcccca ttgacgaaat ggctctggtc gctggccagc atcatcagca
     8641 cggcatccag gtccttcggc tcgcccacgc gcttgcgcgg cagcatgctg atgagcttct
     8701 ggccctgctc ggtctgccag tggtggtggt tgatttcggt gtcgatgtag ccggggcaga
     8761 tggcgttgac gttgatgcca aaacgccccc actccagcgc catggccttg gtcatttgca
     8821 ccaccgccgc cttgctcata cagtagacgc cgatctgcgg cagcaccttc agccccgcca
     8881 tcgacgcgat gttgatgatg cgcccgccgg tgaagctgcc cggcgccgcg cccttggcgc
     8941 gcgcgatcat gcgcttgccc acctcttgcg cgacgaagaa ggcgccgcgc acgttggtgt
     9001 tgaagatgaa gtcgtagtcg tcttccgtca cgtcctgaat gcgctgcgtg gtgctgacgc
     9061 ccgagttgtt gatgaggatg tcgatagagc ccatctcggt ctcggcgtgc gccacggcgc
     9121 ccttgatgga ttgcacgtcg gtcacgtcgc actcgaccac gtgcgcgtcg ccgccctcgc
     9181 cttcgatgcg ggcgcgcaaa tccttcagct tttcgatgcg gcggctggcc agcaccacac
     9241 ccgcgccggc gcgcgccagc gtgcgggcaa attcggcgcc caggccgccc gaggcgccgg
     9301 tgacaaaggc cacgcggccg gacaagtcaa tgctgtagct catggttgtt tttcctgtga
     9361 agaatggggt cggcccacag gccgcaatga tgcccgggtg cgtgcataaa atcggtcacg
     9421 cacccgacac gcccgcccgc gcgcgccaaa gacaatgtcg gtcattattt caaacacgat
     9481 gagaggcgcc ccatgacccc cgaggaaatc atccagcaat acggcccgcg cgaggcgatg
     9541 gaatacgacg ttgtcatcgt cggcgccggc ccggccggtt tgtcggcggc catccgcatc
     9601 aagcagctgg cggccgaaaa aggcaacgac gtgtcggtgg tggtgctcga aaaaggctcc
     9661 gagcccggcg cgcacatcct ctcgggcgca gtgatggacc cgaaggcgct gtccgagctg
     9721 atccccgact ggaaggaaaa aggcgcgccg ctgcaccaac aagtgaccga cgacgcctac
     9781 gtcttcctga acgaaggcgg cggcggtgcg cgcgtgccca acctgctgct gccgccgttt
     9841 gccaacaacc acggcaacta catcgtcagc ttaggcaacg tggtgcgctg gctggccacg
     9901 caggctgaag agctgggcgt ggaaatcttc cccggctttc cggccgccga agtgctctac
     9961 aacgaggatg gcagcgtcaa gggcgtggcg accggcaaca tgggcgtggg caaggacggc
    10021 gagccgaccg agaacttcca gctcggcatg gaactgctgg gcaagtacac catctttgcc
    10081 gagggctcgc gcggcaatct gggcaagcag ctcatcgcgc gctacaagct ggacgcccac
    10141 agcgacccgc aaagctacgc catcggcatc aaggagctgt gggagatcga ccccagccgc
    10201 cacacacccg gcttcgtgat gcacaccgcc ggctggccga tggacgagca cacctacggc
    10261 ggcgccttcc tctaccacgc cgaggacaac aaggtgtatt gcggctacgt ggtgggcctg
    10321 aactacaaga acccctacct gagcccgttc gaggagttcc agcgctggaa gacccacccg
    10381 cgcgtcaagt ggtacttcac cgacgatgcc ggcaacgtca acgc
//
LOCUS       DAOMVO010000052        10417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1550_length_10417_cov_12.653445, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000052 DAOMVO010000000
VERSION     DAOMVO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_10417_cov_12.653445"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKV30_04225"
     CDS             complement(<1..319)
                     /locus_tag="PKV30_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRL29461.1"
                     /translation="MQTHLSPEFHDTLDGQEAEAILRKCVHCGFCLATCPTYQLLGDE
                     LDSPRGRIYLIKQVLEGHEPTRTTQQHLDRCLTCRNCETTCPSGVQYGHLVDIGRQIV
                     DAKV"
     gene            405..923
                     /locus_tag="PKV30_04230"
     CDS             405..923
                     /locus_tag="PKV30_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29462.1"
                     /translation="MTLPPHLPEALHDFICVTEVENGGFFVTELFQRKFGHVPPEFGR
                     HIVAFVRMDNTHLYAASYLHFWQKDSYGLIGGGCTDGTVLRRLSPERLATVNAAGGLL
                     RQTLLYGFQRFAPGLEAFFGHCGDARAKEVDLAAGFLETHDQHLLVRWVGTPTPERQA
                     ALINEVLTMGAF"
     gene            923..1759
                     /locus_tag="PKV30_04235"
     CDS             923..1759
                     /locus_tag="PKV30_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29463.1"
                     /translation="MSRMAVLTYHSQNILGNDYASNDHVALRSDLAELRRRGIRIVSL
                     NHLVDALISGDAAAFPAGTVALSCDDGTVLDWFDYHHPEWGWQPGFDRLLRDHMDDAG
                     TVTEVITAFLIASPQARADIDAGCYGGAALSTSSWWAEAAASGRFYLGNHSWDHAHAC
                     LRELAHTPALRGNFNMVTDAVSADQQLRQAGDFIAAELGTNVLRPRLFAYPYGHATDY
                     LVHEYLPKRRAEHGIDAAFTTEPAYVTPTANRFRLPRFVCGDAWRSSEEFGNILDSLL
                     LI"
     gene            complement(1861..2922)
                     /gene="glcE"
                     /locus_tag="PKV30_04240"
     CDS             complement(1861..2922)
                     /gene="glcE"
                     /locus_tag="PKV30_04240"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HRL29464.1"
                     /translation="MDAALDAVIGKVRQAAARARPLRIRGGGSKDFYGQRLHGELLDV
                     RPIAGVVDYEPSELVVTVRAGTRLRELQALLAERGQCLPFEPPQFGVGSTIGGAVAAG
                     LSGPARASVGSLRDYVLGVQIVNGKGELLTFGGQVMKNVAGYDVSRLMAGSLGTLGVI
                     VQVSLKVLPVPPAEATLLFDLSETSARQQLNRWGGQPLPLNASCWADGHLAVRLRGAQ
                     AAVAAAARSMGGQALAPERAERQWPALRDQALPFFRLAPGEALWRASVPDTATPLNLG
                     PTLVEWHGAQRWIKATPADATHVREAAARAGGHATLFRGGDGTVPVFTPLSEPLTRIQ
                     RQVQQAFDPAGVFGALRMI"
     gene            complement(3076..4437)
                     /gene="amt"
                     /locus_tag="PKV30_04245"
     CDS             complement(3076..4437)
                     /gene="amt"
                     /locus_tag="PKV30_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRL29465.1"
                     /translation="MKTLVSALLLALGLMCFGAAFAQAAPDAATAVAAAAASAPAAAP
                     APHIDSGDTAWMLTSTLLVILMIIPGLALFYGGLARSKNMLSVLMQVFVVFSLVSILW
                     AVYGYSLAFSGEGNFFGGFDKLFLKGVTQQTFGALSTIPEYVFFAFQGTFAAITVALI
                     VGSFAERIKFAAVLLFAALWFTFSYVPMAHIVWGGGLLAKDGALDFAGGTVVHINAGI
                     AGLVGAYMVGKRLGFGREAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANAQAGLAF
                     VNTVLATAAATLAWSAGEALHKGKASMLGAASGAVAGLVAVTPAAGFVGPMGSIVIGA
                     VAGFACIWGVGGLKRMLKADDAFDVFGVHGLGGIIGAILTGVFAAQSLGGTGGASPDT
                     FAMGAQVWIQVKSVLFTIVWSGVVSFIAYKVVDLLVGLRVSEEQEREGLDISSHGETA
                     YNR"
     gene            complement(4458..4796)
                     /gene="glnK"
                     /locus_tag="PKV30_04250"
     CDS             complement(4458..4796)
                     /gene="glnK"
                     /locus_tag="PKV30_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRL29466.1"
                     /translation="MKMVTAVIKPFKLEEVREALSTLGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAVSGDLVERVIEVIEGAARTGKIGDGKIFVSDLEQVVRI
                     RTGETGADAL"
     gene            complement(4857..5687)
                     /locus_tag="PKV30_04255"
     CDS             complement(4857..5687)
                     /locus_tag="PKV30_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRL29467.1"
                     /translation="MGLSAPPRCPRFSRVSLRRLLPPTLLAASTTLVAGSALAQTAAP
                     ADAPAAATTQAPAAAPASPLSFNVGLTTDYRYRGISQSRLKPAISAGADYAHPSGFYV
                     GTWLTSIKWIKDAGGDAPLEWDVYGGYKGTAGPLGYDVGLLHYNYPRHRLAISPNTTE
                     IYGALSYSVVTLKYSHALTNLFGFDDSKGSGYLDLSANFDLGDGWSVVPHVGRQWIRH
                     NSAYAYTDYAVTVNKDFGNGLVASAGVVGTNADKALYVTPSGRFTGRTAVFAAIKYSF
                     "
     gene            5971..7503
                     /locus_tag="PKV30_04260"
     CDS             5971..7503
                     /locus_tag="PKV30_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRL29468.1"
                     /translation="MGLSLVQSRALLGLAAPAVTVEVHLANGLPSFTLVGLADVEVKE
                     ARERVRSALQNSGLDFPHNQRITVNLAPADLPKDSGRFDLPIALGILAASGQIDGARL
                     AGWEFAGELSLSGELRPVRGALAVAAALQQAGVDAQLVLPPGSAEEAALVPGLAVHRA
                     RHLLDVVAQFLPAATPAPPDAGDGFARVQPAPRADGPRYADLADVKGQAAAKRALEIA
                     AAGGHSLLLMGPPGTGKSMLAQRFAGLLPPMSEAEALQSAAVASLGGTFALERWGQRP
                     TRSPHHSASAVALVGGGSPPRPGEISLAHQGVLFLDELPEFPRAALEALREPLESGHI
                     TISRAAQRADFPAAFQLIAAMNPCPCGYLGSRSKPCRCTPDQISRYQGKLSGPLIDRI
                     DLHVEVGALPPDELLASPAGEPTASVRARCVAARERALARQGVSNQALHGQAIDQHAR
                     LDDAARQFLHTAATRLGWSARAMHRALKVARSIADLAGSDAVTTAHVAEAVQYRRALR
                     AA"
     gene            complement(8264..9151)
                     /locus_tag="PKV30_04265"
                     /pseudo
     CDS             complement(8264..9151)
                     /locus_tag="PKV30_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
     gene            9393..>10417
                     /locus_tag="PKV30_04270"
     CDS             9393..>10417
                     /locus_tag="PKV30_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL29469.1"
                     /translation="MLLSRRHCLAATAALLTATAAPVWAQATTPIKFQLDWRFEGPAA
                     LFLQPVAKGYFKQAGLDVTVDAGNGSGGAVTRVASGAYDLGFADLAALMEFHANNPDA
                     PNKPVAVMMVYNNTPAAVFALKKSGIAKPTDLSGKKLGAPVFDAGRRGFPIFAKANGV
                     KDVNWVSMDPPLRETMLVRGDVDAITGFGFTSLLNLEARGVKAADVLLMPYADHGVKL
                     YGNAIIASQKLLKENPGAIKAFLAAFTKGAKEVMANPTAAIDYVKQRDGIVNVPMEVR
                     RLQLAIDTVIASPDARAEGFGQIVPSRLSLMASQVSDAYGTKTRVNPDAVWNGSFLPS
                     KAELNVLP"
BASE COUNT         1704 a         3612 c         3508 g         1593 t
ORIGIN      
        1 gcaccttggc gtcgacgatc tggcggccga tgtcgaccag gtggccatac tgcacaccgc
       61 tcgggcaggt ggtctcgcag ttgcggcagg tcaggcaacg gtccaggtgc tgctgcgtgg
      121 tgcgcgtggg ctcgtggcct tccagcacct gcttgatcag gtagatgcgc ccgcgcgggc
      181 tgtccagttc atcgcccagc agttggtagg tcgggcaggt ggccaggcag aagccgcaat
      241 gcacgcactt gcgcaggatc gcctcggcct cctggccgtc aagggtgtcg tggaattcgg
      301 ggctgagatg ggtttgcaat gggatgggct ccggcagcgg gctgcgacag aaccttagca
      361 cgcggccaaa ggcatacagg ccccggcaca tctacactta ccctatgacc ctaccacctc
      421 acttgcccga agccctgcat gactttatct gtgtaaccga ggtggagaac gggggcttct
      481 ttgtcaccga attgttccag cgcaagttcg gtcatgttcc accagagttc ggtcgtcata
      541 tcgtcgcctt cgtgcgcatg gacaacaccc atctttacgc cgccagctac ctgcatttct
      601 ggcagaaaga cagttacggc ctgatcggcg gcggctgcac tgacggtacg gtgctccgcc
      661 gcctgtcgcc cgagcgcctg gccacggtca acgctgccgg tggactgctg cggcagacgc
      721 tgctgtatgg gttccagcgt ttcgcgcccg ggctggaagc gttcttcggg cactgcggtg
      781 atgcccgcgc aaaagaggtc gatttggccg ccggatttct ggaaacacac gatcaacacc
      841 tgctggtgcg ctgggtcggc acacctacac cagaacggca ggcagcactt atcaacgagg
      901 tgctgaccat gggagccttc tgatgagccg catggccgta ctgacctatc acagccagaa
      961 catcctgggc aacgactacg cgagcaacga ccatgtcgcc ctgcgcagcg atctggccga
     1021 gctgcggcgt cgcggtattc gcatcgtcag cctgaaccat ctggttgatg cgctgatcag
     1081 cggcgatgcg gcggcctttc cggccggcac cgtggcgctc agctgcgatg acggtaccgt
     1141 actcgactgg ttcgactacc accatcccga gtggggttgg cagcctggat tcgaccggct
     1201 attgcgcgat cacatggatg acgccggcac cgtcacagag gtcatcacgg ctttcctgat
     1261 cgcatccccg caggcacgtg ccgatatcga cgccggctgc tacggcggtg cggcgctcag
     1321 cacctcgtca tggtgggcgg aggcggccgc cagcggacgg ttttacctgg gcaatcacag
     1381 ttgggatcac gctcacgcgt gcctgcgcga attggcccac actccggcgt tgcgcggaaa
     1441 tttcaacatg gtgaccgatg cagttagcgc cgaccagcaa ctgcgccaag cgggggactt
     1501 cattgcggcg gaactcggta ccaacgtact acgcccgcga ctgtttgcct acccctatgg
     1561 acacgcaacc gattacctcg tgcacgagta cctgccgaag cggcgtgccg agcacggcat
     1621 cgacgctgcc ttcacgactg agccagccta cgttaccccg accgccaatc gtttccggct
     1681 tccccgattc gtttgcggcg atgcctggcg cagctccgag gaattcggaa atattctcga
     1741 ttcactacta ttaatatagt aacaactaag gtcaaggatt gatcttgcag ccatgtgctg
     1801 aaaatgctgt ttggcagcca taggcatcgc cgatgccttg agttctgcaa gacatgctgc
     1861 tcaaatcatg cgcagcgctc caaacacccc ggctggatcg aaagcctgct gcacctgtcg
     1921 ctggatgcgc gtcagcggct cgctcagcgg cgtgaacacc ggcaccgtgc cgtcgccacc
     1981 gcgaaacagc gtggcgtggc cgccggcgcg cgcggcggct tcgcgcacgt gggtcgcgtc
     2041 ggccggcgtg gccttgatcc agcgctgcgc gccgtgccac tcgacgagcg tcgggcccag
     2101 gttgagcggc gtggcggtgt cgggcacgct ggcgcgccac agcgcctcgc cgggcgccag
     2161 gcggaagaac ggcagcgcct ggtcgcgcag cgcgggccat tgacgctcgg cacgctcggg
     2221 cgccagcgcc tgcccgccca tgctgcgcgc tgccgccgcc acggccgcct gcgcgccgcg
     2281 cagccgcacc gccaagtggc cgtcggccca gcaactggca ttcagcggca gcggctggcc
     2341 gccccagcgg ttgagctgct ggcgggcgct ggtttcagac agatcgaaca gcagcgtcgc
     2401 ttcggccggc ggcacgggca gcaccttcag gctgacctgc acgatcacgc ccagcgtgcc
     2461 cagcgatccc gccatcagcc ggctgacgtc gtaaccagcg acgttcttca tcacctggcc
     2521 gccaaaagtg agcagctcgc ccttgccgtt gacgatctgc acgcccagca cgtagtcgcg
     2581 caggctgccg acgctggcgc gcgccgggcc cgacagcccc gccgccaccg cgccgccgat
     2641 ggtgctgccg acgccgaact gcggtggctc gaaaggcagg cactggccac gctcggccag
     2701 cagcgcctgc agctcgcgca ggcgcgtgcc ggcgcgcacg gtgaccacca gctcgctcgg
     2761 ctcgtaatcg accacgccgg cgatgggccg cacgtcgagc agctcgccat gcaggcgctg
     2821 gccgtagaaa tccttgctgc cgccgccgcg gatgcgcagc ggccgcgcgc gcgcggcggc
     2881 ctggcgaacc ttgccgatga cggcgtcgag ggcggcatcc atggcggcaa tggtaatcgc
     2941 tgcgcggcgc gtgccgtgtc gagcgggaca ctatttattc aatagctgcc cgcgcttgat
     3001 ccaccagcac cggcgcctca aaaagaccat gaaaaaaccc cgccgcagcg gggttcgagg
     3061 ccagccagcg cctggtcagc ggttgtaagc cgtctcgccg tggctgctga tgtccagccc
     3121 ctcgcgctcc tgctcctcgc tcacgcgcag gccgaccaac agatcgacga ccttgtaggc
     3181 gatgaacgag accacgcccg accagacgat ggtgaacagc acgctcttca cctgaatcca
     3241 cacctgcgcg cccatggcaa aggtgtcggg cgaggcgccg ccggtgccac ccaggctttg
     3301 cgcggcaaac accccggtca ggatggcgcc gatgatgccg cccaagccgt gcacgccaaa
     3361 cacgtcgaac gcgtcgtcgg ccttcagcat gcgcttcagg ccacccacgc cccagatgca
     3421 ggcaaagccg gccacggcgc cgatgacgat cgagcccatc gggccgacga agccggccgc
     3481 cggcgtcacc gccaccaggc ccgccaccgc gccggatgcg gcgcccagca tcgaggcctt
     3541 gcccttgtgc agcgcctcgc cggccgacca ggccagcgtg gcggccgccg tggccagcac
     3601 ggtgttgaca aaggccagcc ccgcctgcgc gttggccgcg ccggccgaac cggcgttgaa
     3661 gccgaaccag cccacccaca gcagcgacgc gcccaccatg gtcagcgtca ggctgtgcgg
     3721 cgtgaaggct tcgcgcccaa agcccaggcg cttgccgacc atgtaggcgc ccaccaggcc
     3781 ggcgatgccg gcgttgatgt gcaccacggt gccgccggcg aagtcgagcg cgccgtcttt
     3841 cgccagcagg ccgccgcccc acacgatgtg cgccatcggc acgtagctga aggtgaacca
     3901 cagcgcggca aacagcagca cggcggcgaa cttgatgcgc tcggcaaacg agccgacgat
     3961 cagcgccacg gtgatggcgg caaaggtgcc ctggaaggcg aagaacacgt attcggggat
     4021 ggtggacagc gcaccgaagg tttgctgcgt gacgcccttg aggaagagct tgtcgaagcc
     4081 gccgaagaag ttgccctcgc cgctgaaggc caggctgtag ccgtacaccg cccacaggat
     4141 gctgaccagc gagaacacca cgaacacctg catcagcacg ctcagcatgt tcttgctgcg
     4201 cgccaggccg ccgtagaaca gcgccaggcc gggaatgatc atcaggatga cgagcagcgt
     4261 cgaggtcagc atccaggcgg tgtcacccga atcgatgtgc ggcgcagggg ccgcggcggg
     4321 ggccgatgcg gccgcggcgg caacggcggt ggccgcatcg ggcgcggctt gcgcgaaggc
     4381 ggcgccaaag cacatcaggc ccagcgccag aagcaaggcg gagacaagcg ttttcatggt
     4441 gcgatctctc ctgccgttca cagcgcgtcg gcgccggtct cgccggtgcg gatgcggacg
     4501 acctgttcaa ggtcgctgac gaaaatcttg ccgtcgccga tcttgccggt gcgcgcggcg
     4561 ccttcgatga cttcgatcac gcgctcgacg agatcgcccg acacggcggc ctcgaccttc
     4621 actttgggca ggaaatcgac cacgtattcg gcgccgcggt acagctcggt gtggcccttc
     4681 tggcggccga agcccttgac ctcggtgaca gtgatgccct gcacgcccag cgtggacagc
     4741 gcttcgcgca cttcctccag cttgaagggc ttgatgacgg cggtgaccat tttcatgatg
     4801 ctttcctttt gatagctgct tgcgcttgtc agacaagcgc tggcgcctgt tttgacttaa
     4861 aacgaatact tgatggcggc aaacaccgcg gtgcggccgg tgaagcgccc gctgggcgtg
     4921 acgtacagcg ccttgtcggc gttggtgccg accacgccgg cgctggccac caggccgttg
     4981 ccgaagtcct tgttcacggt gaccgcatag tcggtgtacg cataggcgct gttgtggcga
     5041 atccactggc ggccgacgtg cggcaccacc gaccagccat cgcccagatc gaaattggcc
     5101 gacagatcga ggtagccgct gcccttgctg tcgtcaaagc cgaacaggtt ggtcagcgcg
     5161 tgcgagtact tgagcgtgac cacgctgtag ctcagcgcgc cgtagatctc ggtggtgttg
     5221 gggctgatgg ccaggcggtg gcgcgggtag ttgtagtgca gcaggccgac gtcgtagccc
     5281 agcgggcccg ccgtgccttt gtagccgccg tacacgtccc attccagcgg cgcgtcgccg
     5341 ccggcgtcct tgatccactt gattgaggtc agccaggtgc cgacgtaaaa gccgctcggg
     5401 tgcgcgtagt cggcaccggc gctgatggcg ggcttgaggc ggctttgcga gatgccgcgg
     5461 tagcggtagt cggtggtcag gccgacgttg aaggacagcg ggctggccgg cgcggcggcg
     5521 ggtgcttgcg tcgtggcggc ggcgggcgcg tcggcggggg ccgcggtctg cgccagcgcg
     5581 ctaccggcga ccagcgtggt ggatgccgcg aggagcgtgg gcggcagaag gcgtcgaagc
     5641 gaaacgcgcg aaaaacgagg acagcgcggc ggtgccgaca agcccatagc cgggctatgg
     5701 gcgccagagg tagcgccgtg atggaatgtt ttctcgcgcg ttgcagccgg cgcttcttct
     5761 gccgtacacg ctcctaggcg ccacaatgct tgccgtgtca aagtcatcat cttcctctcc
     5821 agatcgtcga acagggtgtg tcgcaccgtg ttacgcacgg agcgtgccaa gcgcttttgc
     5881 agcgcgcgcc accgtcgaag gcgaccagct gcaccgcctt ggtgcgcgca tggcggcacg
     5941 ttcacgcacc aacacaggga cgggcacgcc atgggcctga gcctggtgca aagccgtgcc
     6001 ttgttggggc tggcggcgcc cgccgtcacg gtcgaggtgc atctggccaa tggcctgccc
     6061 agcttcacgc tggtcgggct ggccgatgtc gaggtgaagg aagcgcgcga gcgcgtgcgc
     6121 agcgcgctgc agaacagcgg gctggacttt ccgcacaacc agcgcatcac cgtcaacctg
     6181 gcgccggccg acttgcccaa ggactcgggt cgcttcgact tgccgattgc gctcggcatc
     6241 ctggccgcca gcgggcagat cgacggtgcg cggctggccg gctgggaatt tgccggcgag
     6301 ctgtcgctgt cgggcgagct gcgcccggtg cgcggcgcgc tggccgtggc ggcggcgctg
     6361 cagcaggcgg gcgtcgacgc gcaactggtg ctgccgcccg gcagcgccga agaagccgcg
     6421 ctggtgcccg gcctggcggt gcaccgcgcg cgccatctgc tcgacgtggt ggcgcagttt
     6481 ctgcccgccg ccacgccggc gccgcccgat gcgggcgacg gttttgcccg cgtgcagccc
     6541 gcgccacgcg ccgacggccc gcgctacgcc gacctggccg acgtcaaagg ccaggccgcc
     6601 gccaagcgcg cgctggagat tgctgcggct ggggggcatt cgctgctgtt gatggggccg
     6661 cccggcaccg gcaagtcgat gctggcgcag cgctttgccg gcctgctgcc gcccatgagc
     6721 gaggccgagg cgctgcaaag cgcggccgtg gccagcctgg gcggcacatt cgcgctcgag
     6781 cgctggggcc agcggccgac gcgttcgccg caccactcgg ccagtgccgt cgccttggtc
     6841 ggcggcggct cgccgccccg gccgggcgag atttcattgg cgcatcaagg cgtgcttttc
     6901 ctggatgaac ttccggagtt tccaagggcc gcgctcgaag ctttgcgcga gccgctggag
     6961 agcggccaca tcaccatcag ccgcgcggcg cagcgggcgg attttccggc ggccttccag
     7021 ctgatcgccg ccatgaaccc ctgtccgtgc ggctacctgg gttcgcgcag caagccgtgc
     7081 cgctgcacgc ccgaccagat ttcgcgctac cagggcaagc tgtcgggccc gctgatcgac
     7141 cgcatcgacc tgcacgtcga agtgggcgcg ctgccgcccg acgaactgct ggcctcgccc
     7201 gctggcgaac ccacggcgag cgtgcgcgcc cgctgcgtcg ccgcgcgcga gcgggccctg
     7261 gcgcgccagg gcgtgtccaa ccaggcgctg cacggccagg caatcgacca gcacgcgcgg
     7321 ctggacgatg cggcgcgcca gttcttgcac accgccgcca cgcgcctggg ctggagcgca
     7381 cgcgccatgc accgcgcgct gaaggtggcg cgcagcattg ccgatctggc gggcagcgac
     7441 gcggtgacca cggcgcacgt ggccgaggcg gtgcagtacc ggcgcgcgct gcgcgcggcg
     7501 tagcgctttg gttttgtgat caaaacaggc tccagcgctt gtccatcaag cgcgagcagc
     7561 tattgaattc atagttttcg cgataggcgg caacgcccgc tcagcgtcgc cagcgcgcgc
     7621 ccagttcatc acgcaaggtc cgcacatcgc gcgccaacac gcgctgcgca tgcgccagcg
     7681 cctgccgttc gcgccagcgg tgccacgccg cgccccagcc gctggcctgg cgctgcacca
     7741 gcgcgtggcc ccagtggcgc agcgcgcggg acgaacgcgc cggcaggtgg ggctgcgcgc
     7801 gctccagttg gtgtgtggcg tcttgcagaa agcgcagggc gtcgcgcagg ccgcgggtgg
     7861 cagcgcgggc gcgtgcgtca gggcgcgcag gctcacgcgc cgcacgcggt ggcgtattcg
     7921 attcaggcgg cgtcggtgac tcggccggcg ctgcacccag ccgacgcgtc agcagatacg
     7981 ccgcgcccag cgacagcgcc acggccacgc cttcgcgcgc ccaagggcgc ggtgcgttgg
     8041 cggcgccgcc tggctcgcgg gctgaactcg cccgctgctc acccagcgcc gccaggttct
     8101 gcgccggcgt gcccgcaggc gccgtcacgt ccgacagcac ggccgccacc gtcgccgtgc
     8161 tgtcggccgc gccctcggcg ccgaccttgc gcacctcgcc ctgcagcgcg gtgacggcgg
     8221 cggccggtgg cggcgtcggc gcggcggaga actcgccgtt tttctcggcc atcacgcgcg
     8281 ccatgctggc gcccagcaac agcaccgccg ccgcgaagta aatccacatc agcagcacca
     8341 ccagcgagcc ggcggcgcca taggccgaca ccacggccgc cgtcgacaga tacagcgcca
     8401 gcgcgtactt gccaatgccg aacaacagcg cccccagcgc cgcgccgcgc accaggtagc
     8461 gcaggcgcgg ctgcggcccc gcgctcatgc gcatcagggc cacgaacaag ccggtggtga
     8521 tggcaaacgc caccacctgg ttgaccaccg ccagcaacac cttcatgtcg aaccggctgc
     8581 cggcccaggc ttccagcagg ctcagcaggc tggcgatgac ggtcgacacc agcatcaaaa
     8641 agccaaacgc caggatgtag gccacgccgc gcagccgcag cgtggccgtg tgccaccagg
     8701 cctgctcggc cgccacgccg gtgccttgcg tccacagccg ctcgaaagcc gattgcagtt
     8761 cggcaaacac gccggtggca cccaccagca gcagcgcaaa ggccagcagc gacgccagca
     8821 agccctggcc cggttgctgc gaactggcaa tcgccgcctt gagcgcctcg gcgccctgct
     8881 ggccgacgat gtggccgatc tgcgtgatca ggctggtctg cagcatctcg cggtcgagcc
     8941 accagccgag cacggcgacg atcagcacca gcagcggcgc caggctgagg atgccgtaga
     9001 aggacatcgc cgcgctcatg cgcaggccgt cggcatcgga ccacagctcg aacgcgcggt
     9061 acaacgggtg caggggtttg agccgcccct gcagcagctt gggcacgctt ggtgcggcgg
     9121 gttcggcggg ttcggcggca atggaagaca tggcccgagt ctaggaaggc gcgcccgcgc
     9181 ggccggccag tggcggccgg caggcgctgt cggcggcttc cgacgcatca cggcggccag
     9241 acgggcccgc ccgccgcatc ccgcatcccg cacccgggcc gataccgaga gccagcgcgc
     9301 cgcggctgtg gcatccttct tgcaagatgc acgcctgctc ggtggcgccc ggcgtcccgc
     9361 cctaccccct ctttcaggaa ggaaaagaac ccatgttgct ctcacgtcgc cactgcctgg
     9421 ccgccaccgc cgcgctgctg accgccaccg ccgcccccgt gtgggcgcag gccaccacgc
     9481 cgatcaagtt tcagctcgac tggcgctttg aaggcccggc ggcgctgttc ttgcagcctg
     9541 tggccaaggg ctacttcaag caggccgggc tggacgtgac agtggacgcc ggcaacggct
     9601 cgggcggcgc ggtgacgcgc gtggcctcgg gcgcctacga cctgggcttt gccgacctgg
     9661 cggcgctgat ggaattccac gccaacaatc cggacgcgcc caacaagccg gtggccgtga
     9721 tgatggtcta caacaacacg ccggcggccg tgttcgcgct caagaaatcg ggcatcgcca
     9781 agccgaccga cctgagcggc aagaagctcg gcgcgccggt gttcgacgcc gggcggcgcg
     9841 gctttccgat ctttgccaag gccaatggcg tgaaagatgt caactgggtc agcatggatc
     9901 cgccgctgcg cgaaaccatg ctggtgcgcg gcgatgtcga cgccatcacc ggcttcggct
     9961 tcacgtcgct gttgaacctg gaagcgcgcg gcgtgaaggc ggccgacgtg ctgctgatgc
    10021 cctatgccga ccacggcgtg aagctgtacg gcaacgccat catcgccagc cagaagctgc
    10081 tgaaggagaa cccgggcgcc atcaaggcct ttctggccgc cttcaccaag ggcgcgaaag
    10141 aggtgatggc caacccgact gcggccatcg actatgtgaa gcagcgcgac ggcatcgtca
    10201 acgtgccgat ggaagtgcgc cgcctgcaac tggccatcga caccgtcatc gccagccccg
    10261 acgcacgtgc cgaaggcttt ggccagatcg tgccgagccg cctgtcgctg atggcctcgc
    10321 aagtgtccga cgcctacggc accaagacgc gcgtgaaccc cgacgcggtg tggaacggca
    10381 gctttcttcc atccaaggcc gagctgaacg tgctgcc
//
LOCUS       DAOMVO010000053        10403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1552_length_10403_cov_12.714148, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000053 DAOMVO010000000
VERSION     DAOMVO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_10403_cov_12.714148"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2275
                     /locus_tag="PKV30_04275"
     CDS             <1..2275
                     /locus_tag="PKV30_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRL29470.1"
                     /translation="GWRDPAVQARLAAPSLDWTAPGAAAEPLQLRGVQATLNGRLADA
                     QLAANGRVQQGERQLDLRLAASGGRTTPHAPLAQSGWRVNLGSWTAGVRDPALGQGVW
                     QLASRGANVPLVWSPASAGQRSGQFEAGAGELTITSPAPTAQALVAWGPVRWRAGELS
                     TTGRLTGLPLQWVERVAGAQLQDAGFTGNIVFNGDWDAKLGQQLRVAANLVRASGDVT
                     VLTTDAETGVQSRVAAGLRDARLTLRSEANALNLNLVWDSERAGTVNGQLRTELAATP
                     GADGSRAWSWPESAPLQGQVQARLPQISAWSVLAPPGWRLRGTLAADARIAGTRGAPL
                     VTGPVTADDVALRSVVDGLEFKDGRLRGRLDGTRLLIDEFSLQGVGEKGSGGSLRASG
                     EAGLVDGRPQARLAATLQQLRASIRADRQVTLSGNVQATLDGRAISADGRLRIDRALI
                     VLPEESRPTLGDDVIVRGPGGKIMYGKEGPGAVARPTSAAGQQAAQQQAQSDAARARS
                     DAQVKRGDAEPLTAKVNVQVDLGEDFRLRGQGIDTRLTGVLALTANGPLTEMPSLKGR
                     VQTVGGTFRAYGQQLIIQRGFITFGGQISNPTLDIVALRPNYTSDQRVGVQVMGTALL
                     PRVRLYSEPALPDNQTLAWLLLGRAAPSTGAEAAMLQTAALALLGGREGRGLAARFGL
                     DELSFTGGDSEGQLADASVTLGKRLSDKLYAAYEHSLSGAGGTLMIFYELSRRWTLRG
                     QAGENTAVDLIYRLSFD"
     gene            2317..2391
                     /locus_tag="PKV30_04280"
     tRNA            2317..2391
                     /locus_tag="PKV30_04280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2350..2352,aa:Arg,seq:cct)
     gene            2418..2711
                     /locus_tag="PKV30_04285"
     CDS             2418..2711
                     /locus_tag="PKV30_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29471.1"
                     /translation="MRATLPALALAAAVTVVAGSGCAASSSGIVPGFDGSFTVSKRGV
                     AATPPATLTKQATQEATAHCTAQGKRFRQVDLKESTPGTLAPQAESELTFVCE"
     gene            2808..4028
                     /gene="gspF"
                     /locus_tag="PKV30_04290"
     CDS             2808..4028
                     /gene="gspF"
                     /locus_tag="PKV30_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HRL29472.1"
                     /translation="MPAYTFEALQADGKSRKGVIEADTAKAARALLRGQALVPLAVDP
                     VAGGAGGSVLSRPMGGGRAFNSTQLAIWTRQLAGLVGSGLPLERSLTALADESEDERQ
                     RNLIANLRAEVNAGSALAKAMAQHPREFSPVYTAVIAAGEQGGHLGTVLERLADDLEE
                     RQALKAKLIGAALYPAIVTLVAIVIVIFLVSYVVPQVATVFAGTKRALPWLTVAMLAL
                     SDFVRGYGWWLLGALVLGAAGARLALRNPAFLQKFDAAWLRLPLIGRLARGYNAARFA
                     STLAMLAGAGVPILRSLQAAAETLNNTAMRADAEDALVMVREGAPLASAMAQKKRFPG
                     LLAMFARLGEQTGQLPLMLQRAANQLSAEVQRRAMALATILEPLLIVVMGVVVMLIVL
                     AVMLPIIQLNQWVK"
     gene            4198..4452
                     /locus_tag="PKV30_04295"
     CDS             4198..4452
                     /locus_tag="PKV30_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRL29473.1"
                     /translation="MIMQTLTSREFNQDTARAKRAANDGPVFITDRGKRAYVLLSNAD
                     YERLAKPPRSIVEALAMAGGEDIEFDPPRMRDFARAADLD"
     gene            4457..4876
                     /locus_tag="PKV30_04300"
     CDS             4457..4876
                     /locus_tag="PKV30_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL29474.1"
                     /translation="MYLLDTNVVSELRRPGKAASAVLAWSRAAVADQLFISSITVFEI
                     ELGVRQKERRDATQGRLLRQWLEMQVLPQFQGRVLDVDIAVARRCAALHSPDPAPERD
                     AWIAATALVHHLTVVSRNTRDFLACGAQVFNPWLAPD"
     gene            4904..5848
                     /locus_tag="PKV30_04305"
     CDS             4904..5848
                     /locus_tag="PKV30_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRL29475.1"
                     /translation="MPATLQDQLVVAISSRALFDFEEENRLFEAGDAAAYMQMQLERI
                     DVPAPPGVAFSLVRKLLAFNSAERQRVEVVILSRNDPASGMRVFRSAHSAGIALERGV
                     FTQGRDPFGYLRPLGAHLFLSANETDVRQALSIGFPAARVAVGSARAGENHPHEVRIA
                     FDGDAVLFADEAEQVFQKKGLAAFQAHEKRKAELPLPDGPFKPLLAALHRLQQAGGSG
                     MRLRTALVTARSAPAHERAIRTLMHWGVGVDEAMFLGGLPKGEFLREFEPDFFFDDQT
                     GHIEHAARHVPAGHVAAGVTNSGGGAETPARRARSNRP"
     gene            5845..6261
                     /locus_tag="PKV30_04310"
     CDS             5845..6261
                     /locus_tag="PKV30_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRL29476.1"
                     /translation="MTFHRPALLGLLAALACAPVLAQTTAVAATKSAPHKGEAQALQW
                     FSMLDANNDGRISLDEAKVAFRLKASLREDFKAADLNGDGYLTQQELRTVADRRRAER
                     HARRQGETAPPPARNAAAAPKSSTAKKAPAAPISPR"
     gene            6300..7847
                     /locus_tag="PKV30_04315"
     CDS             6300..7847
                     /locus_tag="PKV30_04315"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRL29477.1"
                     /translation="MRRRTLVVSPTGEPEWINAMSQRLFPPTARRRAMALLLAALAAC
                     GAIAARADNTPAEAAATYRALSRLGYGPTPALIEQVQRAGGARAWALREIDAARAASA
                     QPVRLGGVAPDFNAPLPEIFERYRDEREQRRERAAAKADAASAVLPTEAPQRYSAGVA
                     RDAAAWRLMSCSRPDVEQPLLARMTEFWFNHLNVSADKGRVKPFVGHYVVNAIRPHAL
                     GKFDELLLASARHPAMLYYLDQAQSVADGTLQGERRRGLNENYARELMELHTLGASGG
                     YTQADVRALARVLTGWTVAPQQPDGFRFAPRLHDGAAKTVLGRPFSSVDPRAAEAEGV
                     AAIRMLSQQPATARRIALRLAQWFVADQPPPALVEQLAQSFQSSGGDIAAVMRTLVQS
                     TAFWGPANKLFKTPYDHACSTLAALGGPQDERDVQRTLATLQAAGQGVQRWATPDGYK
                     TNAATWLAPEALTRRADYAMELARRAPDVAFLTRFVQPATLQRIDAQAPGLRAGLLLA
                     SPDFMAK"
     gene            7918..9144
                     /locus_tag="PKV30_04320"
     CDS             7918..9144
                     /locus_tag="PKV30_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRL29478.1"
                     /translation="MTVDLTRRQWLGAAAAAGMAPRAFAQAVPAARGEGRLVVVFLRG
                     ALDGLSAFVPYADADYARARPSIALPAPDGSDQTALKLDGRFALHPALSPLLPLWQQG
                     LLAVVPAAGSPDPTRSHFDAQLHWETARPGQTSDAPGWLNRLSALGGDDDAVHAIGVG
                     ESNPRILSGPRPVKLVARGKAAQRPGALENARTRGALLDMYGGDDALSKAFRQGAESR
                     LQTAQDLKREMQAADNGAAPPALLTQNAHNLGTLMRQQRALRIGFLSAGGWDTHANQG
                     AATGPLANNLRNLAAALVQLRQDFNQPGDVIVVASEFGRTAAENGTRGTDHGHGNAIW
                     LIGQRVAGGRWHGEWTGLAPANLNEQRDVPVHHDFRAVLAQVLRPGFGLGDSQLADAL
                     PGVRWDGRLDGLMRRG"
     gene            9298..>10403
                     /locus_tag="PKV30_04325"
     CDS             9298..>10403
                     /locus_tag="PKV30_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HRL29479.1"
                     /translation="MPWSDKLARFKHFLRQVWHLSAPYFSTSAEKWKARGLLAAIVAL
                     NLGAVYMLVLLNEWNRVFFDALQNKNQDVFLAQLGRFTWLAMVFIVIAVYRFYLTQLL
                     QVRWRAWMTRDLMHRWLGGQAFYRMELQRYTGAPEPATTDAGLPQPGQRVPDNPDQRL
                     QEDVNLFTTQTVGLSMGFLNAVVTLASFVGVLWGLSGSFSFAAGGSSFTIPGFMVWMA
                     VLYCAVGSVLTHYIGRPLIGLNFEQQKREADFRHHLVRVREYSEAIALDKGERVEEQN
                     LDTRFTAVLANYLRLIAKQKQLVGFTNLFGQAAAVFPFIIAAPRFFSGAIQLGELMQI
                     GNAFDRVQGGLSWFVDNYDSLAAWRATTERLTSF"
BASE COUNT         1556 a         3742 c         3558 g         1547 t
ORIGIN      
        1 cggctggcgc gacccggcgg tgcaggcgcg cctggccgcg ccctcgctcg actggacggc
       61 gcccggcgct gccgccgagc cgctgcaact gcgcggcgtg caagccacgc tgaacggccg
      121 cctggccgat gcccaactgg ccgccaacgg ccgcgtgcag cagggcgagc gccagctcga
      181 tctgcgcctg gccgccagcg gcggccgcac cacgccccat gcaccgctgg cccaatcggg
      241 ctggcgcgtc aacctgggtt cgtggacggc cggcgtgcgc gacccggcgc tgggccaagg
      301 cgtgtggcag ttggccagcc gcggcgccaa tgtgccgctg gtctggtcgc ccgccagcgc
      361 cggccagcgc agcggtcaat ttgaggctgg cgcgggcgag ctcaccatca cctcgccagc
      421 gcccaccgcg caggcgctgg tcgcctgggg cccggtgcgc tggcgcgcgg gcgagctgag
      481 caccaccggg cgcctgaccg gcctgccgct gcagtgggtc gagcgcgtgg ccggcgcgca
      541 actgcaggac gcgggcttca ccggcaacat cgtcttcaac ggcgactggg acgccaagct
      601 gggccagcaa ctgcgcgtgg ccgccaacct ggtgcgcgcc agcggcgacg tgacggtgct
      661 gaccaccgac gccgaaaccg gcgtgcagtc gcgcgtggcg gccggcctgc gcgacgcgcg
      721 cctgaccctg cgcagcgagg ccaatgcgct caacctcaac ctggtgtggg acagcgagcg
      781 cgccggcacc gtgaacggcc agttgcgcac cgagctggcc gccacgcccg gggccgacgg
      841 cagccgcgcc tggtcgtggc ccgaatcggc accgctgcaa ggccaggtgc aggcgcggct
      901 gccgcagatt tcggcctggt cggtgctggc gccgccgggc tggcgcctgc gcggcacgct
      961 ggcggccgat gcgcgcattg ccggcacgcg cggcgcgccg ctggtcactg ggccggtcac
     1021 cgccgacgac gtggcgctgc gctcggtggt cgatgggctg gaattcaagg acggccgcct
     1081 gcgcggccgg ctcgacggaa cgcgcctctt gatcgacgaa ttcagcctgc agggcgtggg
     1141 tgagaaaggc agcggcggca gcctgcgcgc cagcggcgag gccggcctgg tcgatggccg
     1201 cccgcaggcg cgcctggccg ccacgctgca gcagctgcgc gccagcatcc gtgccgaccg
     1261 gcaagtcacc ctctcgggca acgtgcaggc gacgctggac ggccgcgcca tcagcgccga
     1321 tggccgcctg cgcattgacc gcgcgctgat cgtgctgccc gaggaaagcc gcccaacgct
     1381 gggcgacgac gtcatcgtgc gcggccccgg cggcaagatc atgtacggca aggaaggccc
     1441 cggcgccgtc gcccgcccga ccagcgccgc cggccagcag gcggcgcagc agcaggccca
     1501 gagcgacgcc gcgcgcgcgc gcagcgacgc gcaggtcaag cgcggcgacg ccgagccgct
     1561 caccgccaag gtcaacgtgc aggtcgatct gggcgaggac ttccggctgc gcggtcaagg
     1621 catcgacacg cgcctgaccg gtgtgctggc gctgaccgcc aacggcccgc tcactgaaat
     1681 gcccagcctg aagggccgcg tgcagaccgt gggcggcacc ttccgcgcct atgggcagca
     1741 gctcatcatc cagcgcggtt tcatcacctt tggcggccag atcagcaacc cgacgctcga
     1801 catcgtcgcc ctgcgcccca actacaccag cgaccagcgc gtgggcgtac aggtgatggg
     1861 cacggcgctg ttgccgcgcg tgcggctgta ctctgaaccc gcgctgcccg acaaccagac
     1921 cctggcctgg ctgctgctgg gccgcgccgc gcccagcacc ggcgccgaag cggccatgct
     1981 gcagaccgcc gcgctggcgc tgctgggcgg gcgcgaaggc cgcgggctgg cggcgcgttt
     2041 tgggctggac gaactcagct tcaccggcgg cgacagcgaa ggccaactgg ccgacgccag
     2101 cgtcacgctg ggcaagcgcc tgtccgacaa gctgtacgcc gcttacgagc acagcctgtc
     2161 gggcgccggt ggcacgctga tgatcttcta cgagctgtcg cgccgctgga cgctgcgcgg
     2221 ccaggccggt gagaacacgg cggtggatct gatctaccgc ctcagcttcg actaaagcgt
     2281 ggccgaggcc cgtctataca atcgccgcac cctcccgccg ctgtagttca atggatagaa
     2341 cgagcgcctc ctaagcgcta gatacaggtt cgattcctgt cagtggtacc acctttcgtg
     2401 ttgccggaaa gccccgcatg cgcgccacgc tccccgctct cgcccttgcc gccgcggtca
     2461 ccgtggttgc gggcagcggc tgcgccgcca gcagctccgg catcgtgccc ggctttgacg
     2521 gcagcttcac cgtcagcaag cgcggcgtgg ccgccacgcc gcccgccacg ctgaccaagc
     2581 aggccacgca agaagccacg gcgcattgca cggcccaagg caagcgcttt cgccaggtcg
     2641 acttgaagga aagcacgccc ggcacgctgg cgccgcaggc cgagagcgag ttgacctttg
     2701 tctgcgaatg agttgctcgc cgcgcatcga atacttctgt ttttatagct gctcgcgctt
     2761 tatggacgtg cgctgacggc ctttttgatt caaattcttc aagccccgtg cccgcctaca
     2821 ccttcgaagc cctgcaagcc gacggcaagt cccgcaaggg cgtgatcgag gccgacaccg
     2881 ccaaggccgc gcgcgccctg ctgcgcgggc aggcgctggt gccgctggcg gtcgacccgg
     2941 tggccggcgg cgctggcggc tcggtgctgt cgcggcccat gggcggcggc cgcgccttca
     3001 acagcacgca gctcgccatc tggacgcgcc agctcgccgg cctggtcggc tccggcctgc
     3061 cgctggagcg ctcgctgacg gcgctggcgg acgaatcgga agacgaacgc cagcgcaacc
     3121 tgatcgccaa cctgcgcgcc gaggtgaatg ccggctcggc gctggccaag gccatggcgc
     3181 agcacccgcg cgagttctcg cccgtctaca ccgccgtcat cgccgccggc gagcaaggcg
     3241 gccacctggg caccgtgctg gagcgcctgg ccgacgacct ggaagagcgc caggcgctca
     3301 aggccaagct gatcggcgcg gcgctctacc cggccatcgt cacgctggtg gcgatcgtga
     3361 tcgtgatctt tctcgtcagc tacgtggtgc cgcaggtggc caccgtgttt gccggcacca
     3421 agcgtgcgct gccgtggctg acggtggcca tgctggcgct gtcggacttc gtgcggggct
     3481 acggctggtg gctgctgggc gctctggtct tgggagctgc tggcgcacga ttggcgctga
     3541 gaaacccggc ttttcttcaa aaatttgacg ctgcctggct gcgcctgccg ctcatcggcc
     3601 gcttggcgcg cggctacaac gcggcgcgct ttgccagcac cctggccatg ctggccggcg
     3661 ccggcgtgcc gattttgcgc agcctgcagg cggccgccga aacgctcaac aacaccgcca
     3721 tgcgcgccga cgccgaagac gcgctggtca tggtgcgtga aggcgcgccg ctggcgtcgg
     3781 cgatggcgca gaagaagcgc tttcccggcc tgctggccat gttcgcccgc ctgggcgagc
     3841 agaccggcca actgcccctg atgctgcagc gcgccgccaa tcaactgagc gccgaagtgc
     3901 agcgccgcgc catggccctg gccaccatcc tggagccgct gctcatcgtc gtcatgggcg
     3961 tggtggtgat gctgatcgtg ctggcggtga tgctgccgat cattcagctc aatcagtggg
     4021 tgaaatagat cccccctgag tcgcctgcgg cgccttcccc ccggggggac aacgctggcg
     4081 ctcgggggga ccccgagcgc ggcgttcgcg cgcggcctgc tccgcggcca tttgatccag
     4141 cgatcaacgc catgaggctc gatgtagtaa cattgctaca ttcatctttt cgaggagttg
     4201 atcatgcaaa ccctcacctc gcgcgaattc aaccaggaca ccgcccgcgc caagcgcgcc
     4261 gccaacgatg ggccggtgtt catcaccgac cggggcaaac gcgcctatgt gctgttgagc
     4321 aacgctgact atgagcgcct agccaagccg ccgcgctcca tcgtcgaggc gctggcgatg
     4381 gcgggtgggg aagacatcga gttcgatccg ccccgcatgc gcgatttcgc ccgcgccgcc
     4441 gatctggatt gacgcggtgt acctgctcga caccaacgtc gtttccgaac tgcgacgccc
     4501 cggcaaggcc gcgtccgctg tgctggcctg gtcgcgcgcg gcggtggcgg atcagttgtt
     4561 catctcctcg atcacggtgt tcgagattga actgggcgtg cggcaaaagg agcgccgcga
     4621 cgccacgcaa gggcgcctgc tgcggcagtg gctcgaaatg caggtgctgc cgcaatttca
     4681 aggccgcgtg ctggatgtcg atatcgcggt ggcccggcgc tgcgccgcgc tgcacagtcc
     4741 agaccccgca cccgagcgcg atgcctggat cgccgccacg gcgctcgtgc atcacttgac
     4801 ggtggtctct cgcaacacgc gcgacttcct ggcttgcggc gcccaagtat tcaatccctg
     4861 gctggcgccc gactagcaag cgccgccagc tatttttttt gacatgcccg ccaccctgca
     4921 agaccaactg gtcgtcgcca tctcctcgcg cgcgctgttt gattttgagg aagaaaaccg
     4981 cttgttcgag gccggcgatg cggcggctta catgcaaatg cagctcgagc gcatcgacgt
     5041 gccggcgccg ccgggcgtgg cgttttcgct ggtgcgcaag ctgctggcct tcaacagcgc
     5101 cgagcgccag cgggtcgagg tggtcatcct ctcgcgcaac gacccggcca gcggcatgcg
     5161 cgtgttccgc agcgcgcact cggccggcat cgcgcttgag cgcggcgtgt tcacgcaagg
     5221 gcgcgatccg ttcggttatc tgcggccgct gggcgcgcac ctgtttctgt cggccaacga
     5281 gaccgacgtg cggcaggcgc tgtcgattgg ctttccggcc gcgcgcgtgg ccgtgggctc
     5341 ggcgcgcgcg ggcgagaacc acccgcacga ggtgcgcatc gccttcgacg gcgacgctgt
     5401 gctgtttgcc gacgaggccg agcaggtgtt ccagaagaag gggctggccg ccttccaggc
     5461 gcacgaaaag cgcaaagccg agctgccgct gcccgatggt cctttcaagc ccctgctggc
     5521 cgcgctgcac cgcttgcagc aggctggtgg ctcgggcatg cgcctgcgca ccgcgctggt
     5581 cacggcgcgc agcgcgcccg cgcacgagcg cgccatccgc acgctgatgc actggggcgt
     5641 gggcgtggac gaggcaatgt ttctgggcgg cttgcccaag ggcgagtttt tgcgcgagtt
     5701 cgagcccgac tttttcttcg atgaccagac cggccacatc gagcacgccg cgcgccatgt
     5761 gccggccggc cacgtggcgg cgggcgttac aaattcgggc ggcggggcgg aaaccccggc
     5821 acggcgcgcg cgctccaatc ggccatgaca tttcatcgcc ccgccttgct tggcctgctg
     5881 gccgccttgg cctgcgcgcc cgtgctggcg cagaccaccg ccgtggccgc caccaaatcc
     5941 gcgccgcaca agggggaagc ccaggcgctg cagtggttca gcatgctcga cgccaacaac
     6001 gacggccgca tctcgctgga cgaagccaag gtggcgtttc gcctcaaagc ttcgctgcgc
     6061 gaggacttca aggccgccga cctgaatggc gatggctacc tgacgcagca agagctgcgc
     6121 accgtggccg accgccgccg cgccgagcgg cacgcgcggc gccagggcga aaccgccccg
     6181 ccgcccgcgc gcaacgcggc cgccgcgccc aagagcagca ccgccaagaa ggcacccgcc
     6241 gcgccgatat cgccgcggta aagtttgtaa agccgcttcg cacaaggtgg cgcgtgcgca
     6301 tgcgccgccg cacactcgtc gtgtcgccca cgggcgaacc ggagtggatc aacgccatgt
     6361 cgcagcgcct ctttccaccc accgcgcgcc gccgtgccat ggcgctgctg ctggccgcgc
     6421 tggccgcgtg cggcgccatt gccgcgcgcg ccgacaacac gcccgccgaa gcggcagcga
     6481 cctatcgcgc cttgtcgcgc ctgggttacg ggccgacgcc ggccttgatc gagcaggtgc
     6541 agcgcgccgg cggcgcacgc gcgtgggcgc tgcgagagat cgacgccgcg cgcgccgcca
     6601 gcgcacagcc ggtgcggctg ggcggcgtgg cgccggattt caacgccccg ctgcccgaga
     6661 tcttcgagcg ctaccgcgac gaacgcgaac agcgccgcga gcgcgccgcc gccaaggccg
     6721 atgccgcatc cgctgtcctg cccaccgaag ccccccagcg ctacagcgca ggcgtggcgc
     6781 gcgacgccgc cgcctggcgc ctgatgagct gcagccgccc cgacgtcgag cagccgctgc
     6841 tggcgcgcat gaccgagttc tggttcaacc acctcaacgt ctcggccgac aaggggcgcg
     6901 tcaagccctt tgtcggccac tacgtcgtca acgccatccg gccgcacgcg ctgggcaagt
     6961 tcgatgaact gctgctggcc agcgcacggc atccggcgat gctgtactac ctcgaccagg
     7021 cgcagagcgt ggccgacggc acgctgcaag gcgaacgccg gcgcggcctg aacgagaact
     7081 acgcgcgtga gctgatggag ctgcacaccc tgggcgccag cggcggctac acgcaagccg
     7141 acgtgcgcgc gctggcccgc gtgctgaccg gctggacggt ggcgccgcag cagcccgacg
     7201 gctttcgctt tgccccacgc ctgcacgacg gcgccgccaa gacggtgctg ggccgcccct
     7261 tcagcagcgt cgacccgcgc gcggctgagg ccgagggcgt ggccgccatc cgcatgctga
     7321 gccagcagcc cgccaccgcg cgccgcatcg cgctgcggct ggcgcagtgg ttcgtggccg
     7381 atcagccgcc gccggcgctg gtcgaacaac tggcgcagag ctttcagtcc agcggcggag
     7441 acatcgcggc ggtgatgcgc acgctggtgc aatcgaccgc cttctggggc ccggccaata
     7501 aactgttcaa gacgccctac gaccacgcct gctcgacgct cgccgcgctg ggcggcccgc
     7561 aggacgagcg cgacgtgcag cgcacgctgg ccacgctgca ggccgccggc cagggcgtgc
     7621 agcgctgggc cacgcccgac ggctacaaga ccaatgccgc cacctggctg gcgcccgaag
     7681 cactgacgcg gcgcgccgac tacgccatgg aactggcgcg gcgcgcgcct gatgtggcct
     7741 ttctcacccg cttcgtgcag ccggccacgc tgcaacgcat cgatgcgcaa gcgcccggcc
     7801 tgcgcgccgg cctgctgctg gccagcccgg acttcatggc gaagtgaagt tttaaatgaa
     7861 atcggccgct ggcgcgcgtc catccagcgc aagcagctat tgaatcagga gtaacgcatg
     7921 accgtcgacc tgacccgccg ccagtggctc ggcgccgccg cagccgctgg catggcgccg
     7981 cgcgcctttg cccaggccgt gccagccgcg cgtggcgagg ggcggctggt ggtcgtgttt
     8041 ctgcgcggcg cgctcgatgg cctgtcggcc ttcgtgccct atgccgacgc cgactacgcg
     8101 cgtgcccgcc ccagcatcgc gctgccagcg cccgatggca gcgatcaaac cgcgctcaag
     8161 ctcgatggcc gctttgcgct gcatcccgcg ctcagcccgt tgctgccgct gtggcagcag
     8221 ggtttgctgg ccgtggtgcc ggccgccggc tcgcccgacc cgacgcgctc gcacttcgac
     8281 gcccagctgc actgggaaac cgcccgcccc ggccagacca gcgacgcgcc cggctggctg
     8341 aaccggctgt cggccttggg tggcgacgac gacgccgtgc acgccatcgg cgtgggcgaa
     8401 agcaacccgc gcatcctgag cggcccgcgc ccggtcaaac tggtggcgcg cggcaaggcc
     8461 gcccagcgcc ccggcgcgct cgaaaacgcc cgcacgcgcg gtgccctgct cgacatgtat
     8521 ggcggcgacg atgcgctgtc caaagccttc cgccagggcg ccgaaagccg cctgcaaacc
     8581 gcgcaggact tgaagcgcga aatgcaggcc gccgacaacg gcgccgcgcc gcccgccctg
     8641 ctcacgcaaa acgcgcacaa cctcggcacg ctgatgcgcc agcagcgcgc gctgcgcatc
     8701 ggttttctat ccgctggcgg ctgggacacg cacgccaacc agggcgccgc caccggcccg
     8761 ctggcgaaca acctgcgcaa cctggccgcc gcgctggtgc agttgcggca agacttcaat
     8821 caaccgggcg atgtgatcgt cgtcgccagc gaatttggcc gcacggcggc cgaaaacggc
     8881 acgcgcggca ccgaccacgg ccacggcaac gccatttggc tgatcggcca gcgcgtggcc
     8941 ggcggccgct ggcacggcga gtggacgggc ctggcgcccg ccaacctgaa cgagcagcgc
     9001 gatgtgcccg tgcaccacga cttccgcgcc gtgctggcgc aggtgttgcg gccgggcttc
     9061 ggacttggcg acagccaact ggccgacgcg ctgccaggtg tgcgctggga cgggcggctg
     9121 gatgggttga tgcggcgcgg ctgacccgtg aaactcagtt tttgatagct gatcgcgctc
     9181 gcctggcaag cgccagcggc cattttgact tgaaacgtcg acgccgcgcg atttcagccc
     9241 ggcctcagtc tggcaccgct aggatgcggt ctttctgccc cgcaccgaaa gccgcgcatg
     9301 ccctggtccg acaaactcgc ccgcttcaag catttcctgc gtcaggtctg gcacctgtcg
     9361 gcgccctact tcagcacctc ggctgagaag tggaaggcgc gcgggctgct ggctgcgatc
     9421 gtcgcgctca acctgggggc ggtgtacatg ctggtgctgc tcaacgagtg gaaccgcgtg
     9481 ttctttgacg ccctgcagaa caagaaccag gacgtttttc tggcgcagct ggggcgcttc
     9541 acctggctgg cgatggtctt catcgtcatc gccgtctacc gcttctacct cacgcagctg
     9601 ctgcaggtgc gctggcgcgc gtggatgacg cgcgatctga tgcaccgctg gctcggcggc
     9661 caggcgtttt accgcatgga actacagcgc tacaccggcg cgcccgaacc cgccacgacc
     9721 gacgccggcc tgccgcagcc cggccagcgc gtgcccgaca acccggatca gcgcctgcag
     9781 gaagacgtga acctgttcac cacgcaaacg gtgggcctgt cgatgggctt tctgaacgcg
     9841 gtggtcacgc tggccagctt tgtcggcgtg ctgtgggggc tgtcgggcag cttctcgttt
     9901 gcggccggcg gcagcagctt cacgattccc ggcttcatgg tgtggatggc ggtgctgtac
     9961 tgcgcggtgg gcagcgtgct gacgcactac atcggccggc cgttgatcgg cctcaacttc
    10021 gagcagcaaa agcgcgaggc cgattttcgc caccacctgg tgcgggtgcg cgaatacagc
    10081 gaggcgatcg cgctcgacaa gggcgagcgg gtcgaagagc agaacctgga cacgcgcttc
    10141 acggccgtgc tggccaacta cctgcggttg atcgccaagc agaagcagct ggtgggcttt
    10201 accaacctgt tcgggcaggc ggcggcggtg tttcccttca tcatcgcggc gccgcgcttt
    10261 ttcagcggcg ccattcagct gggcgagctg atgcagatcg gcaatgcgtt tgaccgcgtg
    10321 caaggcgggc tgtcgtggtt cgtcgacaac tacgacagcc tggccgcctg gcgcgccacc
    10381 accgagcggc tgaccagttt cga
//
LOCUS       DAOMVO010000054        10283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1590_length_10283_cov_15.124462, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000054 DAOMVO010000000
VERSION     DAOMVO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_10283_cov_15.124462"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKV30_04330"
     CDS             <1..383
                     /locus_tag="PKV30_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL29480.1"
                     /translation="ARGQAGRAVLLLRAQAQTQSGQAAQAAGALQTWVSDHPADAGAW
                     QALAAAHAAQGQTLRALRAEGEVPMAHMDYAGAVDRWRAAQDFSRQSPGSPADQIEAN
                     IVDTRLRAAQEALRQQRLEEAKRR"
     gene            complement(489..830)
                     /locus_tag="PKV30_04335"
     CDS             complement(489..830)
                     /locus_tag="PKV30_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRL29481.1"
                     /translation="MKLILKWLLSAAALLGVAYIYGGVSVASFGTAMIAALVIGLLNM
                     LVRPILVVLTIPVTLITLGLFLFVINALMFWAGSGLVSGFVVNGFWAALLGSLIYSAL
                     GVLIDALLERA"
     gene            complement(892..1614)
                     /locus_tag="PKV30_04340"
     CDS             complement(892..1614)
                     /locus_tag="PKV30_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRL29482.1"
                     /translation="MEFLSPAWWSALMAIILIDLVLAGDNAIVIALAARNLPKHLQKK
                     AIIWGTVGAIGVRTVMTLIVVWLLKIPGLMLVGGLGLLWIAYKLIADQGGGEDEHGPA
                     ASTFWGAMKTIVIADALMGIDNVLGVAGAAHGAMDLVVIGLLISVPIVVFGSTMVLKL
                     VDRFPIIIYLGAAVLAGTAASMIAGEPVLSQWFGTREAPVKVLRYALMALCVAGVLGT
                     AWLATRRKAAALPAQTDADPQA"
     gene            complement(2038..2457)
                     /locus_tag="PKV30_04345"
     CDS             complement(2038..2457)
                     /locus_tag="PKV30_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HRL29483.1"
                     /translation="MDRLHITDLEAALNHWRAVRPAAASGALAPEVAALAEVYGRMVY
                     QRQDEVDGADLPPAARAAWLAWYASTPDTPCIAICSTAQGDALCKGCGRTEDEVQRWP
                     TLSPFARRAVWRRITQQGTAWRFNRYAERAGEAAASR"
     gene            2617..3342
                     /locus_tag="PKV30_04350"
     CDS             2617..3342
                     /locus_tag="PKV30_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29484.1"
                     /translation="MTPETAAPAPHARRWRGWLWLSLLANALLAWWLWRAQPPAPPPP
                     ATPVGESVLLRTPGGRLEISEIQQTESFEVSRDHDVLGVPVGSTFSRIRVPAHYRYHV
                     ELAPEWRVTVQPGGGVRVIAPRVRPTLPVAIDTARIEKESRGLWSMFTGPDQLAALEK
                     SITASLATKAASAPMLARQREAARATVAEFVQKWLMTQTDWKPHAGKKVEVLFADEPI
                     DALDATCGGRPGCAAERLNAAGL"
     gene            complement(3523..3981)
                     /gene="cadR"
                     /locus_tag="PKV30_04355"
     CDS             complement(3523..3981)
                     /gene="cadR"
                     /locus_tag="PKV30_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HRL29485.1"
                     /translation="MKISELAAATDTPVDTIRYYEREGLLPPPARGDNNYRHYDAAHA
                     ERLTLIRQARGLDMSLQEIRTLLAWRDQPDADCGAVNALLDEHIGHIATRIRELRALE
                     RQLKALRAQCRQASDTAHCGILSGLTQPGAAPAPVPAAARHVHGVHGRRL"
     gene            4217..6481
                     /locus_tag="PKV30_04360"
     CDS             4217..6481
                     /locus_tag="PKV30_04360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRL29486.1"
                     /translation="MTTESITWRIPNMDCAAEESEIRHALRDVAGVQRLAFRLGAREL
                     RVDAAPGALPEVAQRLRAAGFAPQVPGAPKAAAAEGPACDSAACGPMQLSTHDRQHDD
                     HGHGHEHDHHNDHDHADEAATGWRTLLLAPGLPRIGAALVLALAAEAVHALAPGGWAA
                     VGMGLAVAAVLLSGLSTYLKGVNALRRGRLNISALMAVAVTGALLIGQWPEAAMVMAL
                     YALAEHLEARAVDRARGAIGGLLQMAPDTVEQRAADDTWQAVAAAEVPVGAVMRVKPG
                     ARLALDGVVTAGQSAIDQSPVTGESLPVDKAPGDAVYAGTLNQSGVLEVRVTAAAQDT
                     QLARIIHAVEQAQSERAPTQAFIDRFAAVYTPAVFVLALGVALLGPWLAGWTWLTAIY
                     KALVLLVIACPCALVIATPVTVVSALANAARRGVLVKGGVYLENARRLKAVALDKTGT
                     LTEGKPVLVASEILSKTELSAHVLPAAAALAAQSDHPVSRAIAQGLASQDVTASEVTQ
                     FIDRPGSGVEGVVSGQRLRLVSVRALEGTDLVSAELTERLAVHQAAGRSVSLLVSDTE
                     VLALFAVADTLKDHAPDAVRELHALGVKTVMLSGDHAAAAQAVAAAAGVAEAHGQLMP
                     ADKLTRIAELQARSGPTAMVGDGINDAPALAAADIGIAMGAAGTDTAMEAADVVVMND
                     DLRRLPGLIRLSRRTHAVLWQNIALALGIKAVFLVLALAGSATMWTAVFADMGASLIV
                     VANGLRLLRARTDA"
     gene            complement(6702..7502)
                     /locus_tag="PKV30_04365"
     CDS             complement(6702..7502)
                     /locus_tag="PKV30_04365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL29487.1"
                     /translation="MARRRRAGTLGQALGWFAVLALLLLGATYALRRAEAPFWVERPA
                     SAPTPPRTPASQPAPPAPAPSADGTPTAAEAADKAKREADARARDAASAARDAPNQAA
                     GAAARASDARPLPEDTARLAARQLTLPVQGVLPAQLRDTYTDARASGARRHDAIDIMA
                     AEGTPVLAVEDGRIAKLFWSAGGGGTTIYQFDPSEQYAYYYAHLQRYADGLKEGQMLK
                     RGQLIGYVGHTGNASADAPHLHFAVFKLGPDKRWWKGEALNPYPLWRP"
     gene            complement(7527..8528)
                     /locus_tag="PKV30_04370"
     CDS             complement(7527..8528)
                     /locus_tag="PKV30_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRL29488.1"
                     /translation="MSLSRRLVLTGAALSALPAWAKKPVKAAASETLDFDAFNAATDS
                     PALKMGSRGAAVARAQILLDRAWFSPGEIDGGFGKNMQRMVKAYQRSNGLKESGVIDA
                     DTWKSLREASPDAYLTKYTVTDKDAAGPFEKTPTDMAERAKLKALSYQSLDEALAERF
                     HVGPAYLQRLNPGKKLAAGTEIVVPNVLDSKAPTRASQRIEIDKGERVLFVLDKDGKP
                     AAGFPISIGNEKNDPLPLGEMAIKNAVDMPSFTYNPALLKTAPKDAEKVEIAPGPNNP
                     VGTIWLGLTKPHWGIHGTPEPAKVGHSETNGCIHLTNWDAERLAKIVKVGAKVDVKA"
     gene            complement(8697..9698)
                     /gene="gap"
                     /locus_tag="PKV30_04375"
     CDS             complement(8697..9698)
                     /gene="gap"
                     /locus_tag="PKV30_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRL29489.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVANFDDIEIVGINDLLEPDYLAY
                     MLTYDSVHGRFKGDVKVDGNTLIVNGKKIRLTQEKDPANLKWGEVGAEVVIESTGLFL
                     TKETAQKHIDAGAKKVIMSAPSKDDTPMFVHGVNCAKYAGEPIISNASCTTNCLAPLA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VLPDLKGKLTGMSFRVPTSDVSVVDLTVELEKPATYAEICAEMKAQSEGKMKGILGYT
                     EDKVVATDFRGDARSSIFDEDAGIALDNTFVKLVSWYDNEWGYSNRCLDMVRVVTKK"
     gene            complement(9780..>10283)
                     /locus_tag="PKV30_04380"
     CDS             complement(9780..>10283)
                     /locus_tag="PKV30_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRL29490.1"
                     /translation="TAIAWAVALENKDRPTALLLSRQNLPYAPKRDLGDISRGAYVLS
                     EPSDVGLKGKAQAVLIATGSEVQLALKAQELLASQKIAVRVVSMPSTSAFDRQTVAYK
                     QSVLPDGLPRIAVEMGVTDGWWKYGCAAVVGIDRYGESAPAPKLFEFFGFTPENVADT
                     VRKVLRG"
BASE COUNT         1553 a         3438 c         3621 g         1671 t
ORIGIN      
        1 atgcgcgcgg ccaggcaggc cgcgccgtgc tgctgctgcg cgcgcaggcg caaacgcaaa
       61 gcggccaggc ggcgcaggcc gccggcgcgc tgcaaacctg ggtcagcgac cacccggccg
      121 atgccggcgc ctggcaggcg ctggcggccg cgcacgcggc gcaggggcag acgctgcgcg
      181 cgctgcgcgc cgagggcgag gtgccgatgg cgcacatgga ctacgccggc gcggtcgacc
      241 gctggcgcgc agcgcaggat ttttcgcgcc agtcgcccgg cagcccggcc gaccagatcg
      301 aggcgaacat cgtcgacact cggctgcgcg cggcgcaaga ggcactcagg cagcagcggc
      361 tggaagaggc caagcggcgc tgattcggca gcccgaaaaa aagccgcccg caggcggctg
      421 tttgttgatc aaatcaggcg ttagcgcttg tccatcaagc gcgagcagct atcaataaaa
      481 tagcagcgtc aagcccgctc cagcagcgca tcaatcagca cgccgagcgc cgaatagatc
      541 agcgaaccca gcagcgcggc ccagaagccg ttgacgacaa agccgctgac cagccccgag
      601 ccggcccaga acatcagcgc gttgatgacg aacaagaaca gcccgagcgt gatgagcgtg
      661 accggaatcg tcagcaccac cagaatcggc cgcaccagca tgttcagcag gccgatgacc
      721 agcgcggcga tcatggcggt gccaaagctc gccacgctga cgccgccgta gatgtaggcg
      781 acgcccagca atgcggccgc cgacagcagc catttcagaa tcagtttcag catgatggcg
      841 ccatcatagc gcccggcgga cgggtgccgc caccgtgcgc agcgtggtcg atcaggcctg
      901 cgggtcggcg tcggtctggg ccggcagcgc cgccgccttg cgccgcgtgg ccagccacgc
      961 ggtgcccagc acgccggcga cgcacagcgc catcaaggcg tagcgcagca ccttcactgg
     1021 cgcctcgcgc gtgccaaacc actgcgacag caccggctcg cccgcgatca tcgacgccgc
     1081 cgtgccggcc agcaccgccg cgcccaggta gatgatgatc gggaagcggt cgaccagctt
     1141 cagcaccatg gtcgagccga acaccacgat cggcacgctg atgagcaggc cgatgaccac
     1201 caggtccatc gcgccatgtg ccgcgccggc cacgcccagc acgttgtcga tgcccatcag
     1261 cgcgtcggcg atgacgatgg ttttcatggc gccccagaag gtgctggccg ccgggccgtg
     1321 ttcatcttcg ccgccgccct ggtcggcgat cagcttgtag gcgatccaca gcaggcccag
     1381 gccgccgacc agcatcaggc ccgggatctt gagcagccag accacgatca gcgtcatcac
     1441 cgtgcgcacg ccgatggcgc ccacggtgcc ccagatgatg gccttcttct gcaggtgctt
     1501 gggcaggttg cgcgcggcca gggcgatgac gatggcgttg tcgccggcca gcaccaggtc
     1561 gatcaggatg atggccatca gcgcggacca ccaggcgggc gagaggaatt ccatggttga
     1621 cagtctccga agtcgggttc gagttgaatt ttcgaccgcc gcggccgggc gcagacatgg
     1681 gcaggtgagg cgaggcacac cgcaagaccc aggacgtcga ccaaccgcgc gcgggcgggg
     1741 gttgatcgaa ggtcttgctc ggcagcgcct gggcactgcc cgcggggccg gaagcgcgag
     1801 cggtttgact accgcaagac ttcgtattga cgacccggcg atgccgcact ggctttgaca
     1861 aaccccgggt tcttgggcga acccgccagc gcggcgagag ggagctactc cccttcggaa
     1921 cgcgcgatta aagcatggtg atggcgcact ggcaagccaa gtgtgcagcc gatttcaagc
     1981 caaatcggcc gccagcgctt gtctggcgtg cgcaagatgc tatcaaaaca ggatttatca
     2041 gcggctggcg gccgcctcgc ccgcgcgctc ggcatagcgg ttgaagcgcc aggccgtgcc
     2101 ctgctgcgtg atgcggcgcc acaccgcgcg cctggcaaag ggcgagaggg tgggccagcg
     2161 ctgcacctcg tcctcggtgc ggccgcagcc cttgcacagc gcgtcgccct gggcggtgga
     2221 gcagatggcg atgcacggcg tgtcgggcgt gctggcgtac caggccagcc aggcggcgcg
     2281 cgcggcgggc ggcaggtcag cgccgtcgac ctcgtcctgc cgctggtaga ccatgcgccc
     2341 atacacctcg gccagcgccg ccacctcggg cgccagcgcg ccgctggcgg cggcggggcg
     2401 cacggcgcgc cagtggttca gcgcggcttc caggtcggtg atgtgcaggc ggtccatgcg
     2461 aaggggcatc ataagaggcg gtgggcggcg tggtgtctgg cggcacttct ggcccgttgg
     2521 ccactccatg tgttgacgct gcgtgttgct gtacgctgat ttttagtatt gaattgatag
     2581 ctgcttgcgc ttgccagaca agcgccaagg cccggaatga cccctgaaac cgctgccccc
     2641 gcaccgcatg cccgtcgctg gcgcggctgg ctctggctga gcctgctggc caacgcgctg
     2701 ctggcgtggt ggctgtggcg cgcccaaccg cccgcgccgc cgccgcccgc cacgccggtc
     2761 ggcgaatcgg tgctgctgcg cacgcccggc gggcggctgg aaatctctga aatccagcag
     2821 accgagagct tcgaagtctc acgcgaccac gacgtgctgg gcgtgcccgt gggcagcacc
     2881 ttctcgcgca tccgcgtgcc ggcgcactac cgctaccacg tcgagctggc gcccgaatgg
     2941 cgcgtcaccg tgcagcccgg cggcggcgtg cgcgtgatcg ccccgcgcgt gcggccgacg
     3001 ctgccggtgg ccatcgacac cgcgcgcatc gagaaggaat cgcgcggcct gtggtcgatg
     3061 ttcaccgggc cggatcaact ggcggcgctg gagaaatcca tcaccgcctc gctcgccacc
     3121 aaggccgcca gcgcgccgat gctggcgcgc cagcgcgagg cggcgcgcgc caccgtggcc
     3181 gagttcgtgc agaagtggct gatgacgcag accgactgga agccgcacgc cggcaagaag
     3241 gtcgaggtgc tgtttgccga cgagccgatc gacgcgctgg acgccacttg cggcgggcgg
     3301 ccgggctgcg cggcggagcg cttgaacgcg gcggggcttt gagggtggtt tgggccgctg
     3361 gcgcttgaga gacaagcgcg agcagctatg atttttgatg attcgcctgc ggcgcatgac
     3421 cgcggcctgc agccttcgat gacagcgctg cgcgccatga cccagagccg ttggtcattt
     3481 ggtcgtcgaa gcgaagcgaa gtcattccgt catcaagacc agtcacagcc tccgtccatg
     3541 caccccgtgc acatgccgcg ccgccgctgg caccggggcg ggcgccgcgc cgggctgcgt
     3601 cagcccgctc aagatgccgc agtgcgccgt gtcgctggcc tgccggcact gcgcgcgcag
     3661 cgccttgagc tgcctttcca gcgcgcgcag ctcgcggatg cgggtggcga tgtggccgat
     3721 gtgctcgtcc agcagcgcgt tgacggcgcc gcagtcggcg tccggctggt cgcgccaggc
     3781 cagcagggtg cggatctcct gcagcgacat gtccagcccg cgcgcctggc ggatcagcgt
     3841 caggcgctcg gcgtgcgcgg cgtcgtagtg gcggtagttg ttgtcgcccc gcgccggcgg
     3901 cggcagcagt ccttcgcgct cgtagtagcg gatggtgtcg accggcgtgt cggtcgcggc
     3961 ggcgagttcg ctgatcttca tgggggcatg gtagccatcc atcggcccgc ctgcagcccg
     4021 caaactcaaa gagcccgatg gccgcggagc aggccatgcg cgggcacccg ctgcaagggc
     4081 ttggccgccc gctgggtgcg tccccctgcc cgctcagcgc agcgatgcga gagaaggggg
     4141 aagcggcgtc agccgctcag ggggatgttg cgaatcctct tgacgttgga gttgatccag
     4201 ggtttttaat cttggcatga cgactgaaag catcacctgg cgcatcccca acatggattg
     4261 cgccgccgaa gaaagcgaga ttcgccacgc cctgcgcgac gtggccggcg tgcagcggct
     4321 ggcgtttcgc ctcggcgcgc gcgagctgcg tgtggacgcc gcgccaggcg cgctgcccga
     4381 ggtggcccag cgactgcgcg cagcaggctt tgcgccccag gtgccgggcg cgcccaaggc
     4441 cgcggcagcc gaaggcccag cgtgcgattc ggctgcctgc ggcccgatgc agctgtcgac
     4501 gcacgaccgc cagcacgatg accacggtca cggccacgag cacgatcatc acaacgacca
     4561 cgaccacgcc gacgaagccg ccaccggctg gcgcaccctg ctgctggccc ccggcctgcc
     4621 gcgcatcggc gccgcgctgg tgctggcgct ggccgccgaa gccgtgcatg cgctggcgcc
     4681 cggcggctgg gcggcggtcg gcatggggct ggcggtggcg gcggtgctgc tgtcgggcct
     4741 gtcgacttac ctgaagggcg tcaacgcgct gcgccgcggg cgcctgaaca tcagcgcgct
     4801 gatggcggtg gccgtcaccg gcgcgctgct gatcggccag tggcccgagg cggcgatggt
     4861 gatggcgctg tacgcgctgg ccgagcatct ggaggcgcgc gccgtcgacc gcgcgcgcgg
     4921 cgccattggc ggcctgctgc agatggcgcc cgatacggtg gagcagcgcg ctgccgacga
     4981 cacctggcag gcggtggccg cggccgaggt gccggtgggc gccgtcatgc gcgtcaagcc
     5041 cggcgcgcgg ctggcgctgg acggcgtggt gacggccggt caaagcgcca ttgaccagtc
     5101 gcccgtcact ggcgaaagcc tgccggtcga caaggcgccg ggcgacgcgg tgtacgccgg
     5161 cacgctgaac cagagcggcg tgctggaagt gcgcgtgacg gctgccgcgc aagacacgca
     5221 actggcccgc atcatccacg ccgtcgagca ggcgcaaagc gagcgcgcgc ccacgcaggc
     5281 cttcatcgac cgctttgccg ccgtctacac gccggcggtg ttcgtgctgg cgctgggcgt
     5341 ggcgctgctg gggccgtggc tggcgggttg gacgtggctc acggcgattt acaaggcgct
     5401 ggtgctgctg gtgattgcct gcccgtgtgc gctggtgatt gccacgccgg tcaccgtggt
     5461 cagcgcgctg gccaacgcag cgcggcgcgg cgtgctggtc aagggcgggg tgtatctgga
     5521 aaacgcgcgc cgactgaagg ccgtggcgct cgacaagacc ggcacgctga ccgagggcaa
     5581 gccggtgctg gtggcgtctg aaatactatc gaaaacagag ctgtcagcgc atgtcctgcc
     5641 tgcggcggcg gcacttgcgg cgcaaagcga tcaccccgtg tcgcgcgcca tcgcgcaggg
     5701 gctggcgtct caggacgtga ccgccagcga agtgacgcag ttcatcgacc gccccggcag
     5761 cggcgtcgaa ggcgtggtca gcggccagcg gctgcgcctg gtcagcgtgc gcgcgctgga
     5821 gggcaccgat ctcgtcagcg ccgagctgac cgagcgcctg gccgtgcacc aggcggcggg
     5881 ccgcagcgtg tcgctgctgg tgtcggacac ggaagtgctg gcgctgtttg ccgttgccga
     5941 cacgctgaaa gaccacgcgc ccgacgccgt gcgcgagctg cacgcgctgg gcgtcaagac
     6001 cgtgatgctc tccggcgacc acgccgccgc cgcacaagcc gtggccgccg ctgccggcgt
     6061 ggccgaagcg cacggccagc tgatgccggc cgacaagctg acccgcatcg ccgagctgca
     6121 agcgcgctca ggtcccaccg cgatggtcgg cgacggcatc aacgacgcgc ccgcgctggc
     6181 cgccgccgac atcggcatcg ccatgggcgc cgccggcacc gacaccgcga tggaagcggc
     6241 cgacgtggtg gtgatgaacg acgatttgcg ccgcctgccc ggcctgatcc gcctgtcgcg
     6301 ccgcacgcac gcggtgctgt ggcagaacat cgcgctggcg ctgggcatca aggcggtgtt
     6361 tctggtgctg gcgctggccg gcagcgcgac catgtggacg gccgtgtttg ccgacatggg
     6421 cgccagtttg atcgtggtgg ccaatgggct gcggctgctg cgggcacgga ctgatgcgtg
     6481 agctgcgctg gatcgctatc ctaaagatag ctgttggcgc ttgttggaca agcgccagca
     6541 gctcttttag ctagatcttc aacaatccaa ctccgttcgt cctgagcctg tcgaaggatt
     6601 cggcgttaga gaaatgggct tcgacagggt cagcccgaac ggatggggag cgttggcttg
     6661 ttcatggcat cagctgtgta gagatcaatc gtgccggcgc ctcacggccg ccacagtgga
     6721 tacgggttca gcgcctcgcc tttccaccag cgcttgtcgg gccccagctt gaagacggca
     6781 aagtgcaggt gcggcgcatc cgcgctggcg ttgccggtgt gccccacgta gccgatcaac
     6841 tggccgcgct tcaacatctg gccttccttc aggccgtcgg cgtagcgctg caggtgggcg
     6901 tagtaatagg cgtactgctc gctggggtcg aactggtaga tcgtggtgcc gccgccgccc
     6961 gcgctccaga acagcttggc gatgcggccg tcttccaccg ccagcaccgg cgtgccctcg
     7021 gccgccatga tgtcgatggc gtcgtggcgc cgcgcgccgc tggcgcgcgc gtcggtgtag
     7081 gtgtcgcgca actgcgccgg cagaacgcct tgtaccggca gcgtgagttg gcgcgcggcc
     7141 aggcgcgcgg tgtcttcggg cagcgggcgc gcgtcgctgg cccgggccgc cgctccggcc
     7201 gcctgattgg gggcatcgcg cgccgcgctg gccgcatcgc gcgcacgggc gtcggcctcg
     7261 cgcttggcct tgtcagctgc ttcggcggcc gtcggggtgc cgtcggcact gggagccggc
     7321 gcggggggcg cgggctgcga ggccggcgta cgcggcggcg tcggtgccga ggcgggccgc
     7381 tcgacccaga acggcgcctc ggcgcggcgc agcgcatacg tcgcgcccag cagcaacagt
     7441 gccaggacgg cgaaccagcc cagcgcctga cccagggtgc cggcgcggcg gcggcgtgcc
     7501 aagctctcca gctcctcttg gcggcgtcag gccttgacgt ccaccttggc gccgactttg
     7561 acgatcttgg ccagccgctc ggcgtcccag ttggtcaggt ggatgcagcc gttggtctcg
     7621 gaatggccga ccttggccgg ctcgggcgtg ccgtgaatgc cccagtgcgg cttggtcagg
     7681 ccgagccaga tggtgcccac cgggttgttg gggccaggcg caatctccac cttctcggcg
     7741 tccttcggtg ccgtcttgag cagcgccggg ttgtaggtga agctgggcat gtcgaccgcg
     7801 ttcttgatcg ccatttcgcc cagcggcagc gggtcgttct tctcgttgcc tatgctgatc
     7861 ggaaagcccg cggccggctt gccgtccttg tccagcacga acagcacgcg ctcgcccttg
     7921 tcgatttcga tgcgctggct ggcgcgcgtg ggcgctttgc tgtccagcac gttgggcacc
     7981 acgatctcgg tgccggcagc cagcttcttg cccgggttca ggcgctgcaa gtaagcgggc
     8041 ccgacgtgaa agcgctcggc cagcgcctcg tccagcgact ggtacgacag cgccttgagc
     8101 ttggcgcgct cggccatgtc ggtgggcgtt ttctcgaacg ggccggcggc gtccttgtcg
     8161 gtcaccgtgt acttggtcag gtaggcatcg gggctggcct cgcgcagcga cttccaggtg
     8221 tcggcgtcga tgacgccgct ttccttcagc ccattcgagc gctggtaggc cttgaccatg
     8281 cgctgcatgt tcttgccgaa gccgccgtcg atctcgccag gcgagaacca ggcgcggtcc
     8341 agcaagatct gcgcgcgcgc cacggccgcg ccgcggctgc ccatcttgag cgccgggctg
     8401 tcggtggcgg cgttgaaggc gtcgaaatcc agcgtttcgc tggcggccgc cttgacgggc
     8461 ttcttggccc aggcgggcag ggcggacaag gcggcgccgg tcagcaccag gcggcgggaa
     8521 agtgacatgg aatggctcct gcgaggctcg ttttgaaaca agcctgcagt ctgcgatctg
     8581 ccggccttgc cgcgcgtagg aaaccgccgg cttcgcccgg cggcgcaagg cgcatgcgcg
     8641 cacgaaaaag cccgcggccg tttccgggcg cgggccatgg gctggcgggg tgaagcttat
     8701 tttttcgtga ccacgcgcac catgtcgagg cagcggttgg agtagcccca ctcgttgtcg
     8761 taccagctca ccagcttgac gaaggtgttg tccagcgcga tgccggcgtc ctcgtcgaag
     8821 atcgagctgc gcgcatcacc gcgaaaatcg gtggccacca ccttgtcttc ggtgtaaccc
     8881 aggatgccct tcatcttgcc ttcgctctgc gccttcatct cggcgcagat ttcagcgtag
     8941 gtggcgggct tttccagttc gaccgtcagg tcgaccaccg acacgtcgga cgtcggcacg
     9001 cggaaagaca tgcccgtcag cttgcccttc agatcaggca gcaccacgcc gacggccttg
     9061 gcggcaccgg tggacgaggg gatgatgttc tccagaatgc cgcggccgcc gcgccagtcc
     9121 ttgttgctgg ggccgtccac ggttttctgc gtggcggtcg ccgcgtgcac cgtggtcatc
     9181 aggccgcgct tgatgcccca cttgtcgttc agcaccttgg ccagcggcgc caggcagttg
     9241 gtggtgcacg acgcgttgct gatgatcggc tcgcccgcgt acttggcgca gttcacgccg
     9301 tgcacgaaca tgggcgtgtc gtccttcgag ggcgccgaca tgatgacctt cttggcaccg
     9361 gcgtcgatgt gcttttgcgc tgtctccttg gtcaggaaca ggccggtgga ttcgatgacc
     9421 acttcggcgc cgacttcgcc ccacttcagg ttggcggggt ccttctcttg cgtcaggcga
     9481 atcttcttgc cgttgacgat gagcgtgttg ccatcgacct tcacgtcgcc cttgaagcgg
     9541 ccgtgcacgc tgtcgtaggt aagcatatag gccagatagt ccggctccag caggtcgttg
     9601 atgccgacga tctcgatgtc gtcaaagttc gccaccgccg cgcgaaacac catgcgcccg
     9661 atgcggccga agccgttgat gccgattttg atggtcattg cagtttcctt ttttgctaga
     9721 tgattgatag ctgcttgcgc acgtccagca agctctggag gccaatcagg cattaaaaat
     9781 cagccgcgca gcaccttgcg cacggtgtcg gccacgtttt caggcgtgaa gccaaagaac
     9841 tcgaacagct tgggcgcggg cgccgattcg ccgtagcggt cgatgccgac cacggcggcg
     9901 cagccgtatt tccaccagcc gtcggtcacg cccatttcca cggcgatgcg cggcaggccg
     9961 tcaggcagca cgctttgctt gtaggccacg gtttggcgat caaaagcact ggtactcggc
    10021 atggacacca cgcggacagc tatcttttgc gaagcaagca gttcctgcgc cttgagcgcc
    10081 agttgcactt cggagccggt ggcgatgagc accgcctggg cctttccttt caggcccacg
    10141 tcgctaggct cgctcaagac ataggcgcca cggctgatgt cgcccagatc gcgcttgggg
    10201 gcatagggca ggttctggcg cgacagcagc agcgccgtgg ggcggtcctt gttctccagc
    10261 gccacggccc aggcgatggc ggt
//
LOCUS       DAOMVO010000055        10276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1592_length_10276_cov_17.266901, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000055 DAOMVO010000000
VERSION     DAOMVO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_10276_cov_17.266901"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PKV30_04385"
     CDS             complement(<1..709)
                     /locus_tag="PKV30_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRL29491.1"
                     /translation="MTFAIHGLPVARGIALGRAVLVASSRVDVAHYFIESDQIKSEVE
                     RLRVARDAVIEELQRLQATMPKDAPPELAAMLDVHLMLLEDESMAYEIRRWIKERLYN
                     AEWALVSQLEVVVRQFDEMEDEYLRERKADLEQVVERVLRHLKGAATPVAAPPPRAPR
                     QQDLGLDDTQDVPLVLVAHDLSPADMLQFKKSVFAGFVTDVGGKTSHTAIVARSMDIP
                     AVVGARSASQLVRQDDWV"
     gene            complement(830..1099)
                     /locus_tag="PKV30_04390"
     CDS             complement(830..1099)
                     /locus_tag="PKV30_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRL29492.1"
                     /translation="MIKTDTTITNKLGLHARASAKLTKLAGSYPCEVWMSRGERRVNA
                     KSIMGVMMLAAGIGSVVTVETDGPQEQEAMDALLALIADKFGEGE"
     gene            complement(1068..1514)
                     /locus_tag="PKV30_04395"
     CDS             complement(1068..1514)
                     /locus_tag="PKV30_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HRL29493.1"
                     /translation="MNAVLLIAHAPLANALRQGALHVFPDAAADIAALDVDPDAPPEQ
                     TLAAAEALIAQLGERPVLVLSDVFGATPCNVAQRLCEVDRVRLLAGVNLPMLLRAVSY
                     RAESLDAMAQRALAGGVQGVMPVASAAPQNQSRKVPHDQDRHDHHQ"
     gene            complement(1522..2778)
                     /locus_tag="PKV30_04400"
     CDS             complement(1522..2778)
                     /locus_tag="PKV30_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL29494.1"
                     /translation="MNAPPEPLPRRTAVIIFLAFAGAYFLSALLRAVTATLSPVLTAE
                     FSLHARDLGLLAGGYFLGFAAVQLPLGAWLDRHGPKKVILGLLSVAVLGCVAFALAGG
                     FSGLMAARLLMGVGVAACLMAPLTGYRRWFDPGTQLRANSWMLMTGSLGMLASTLPVQ
                     WLLPLVGWRALFWGLAALVLLAMAVIAWAVPRWRAVAPQNIAAGADQTSANGQKGSYS
                     EIWRNPYFRRASPLGMVSFGGMVAIQSLWAGPWLTRVAGATPLQAATGLFWINLGMLV
                     TFWSWGLLLPRMAARGISVDRMVARVQPFSFVVLAALVAWGPAAGGFSFVLLALYCML
                     STPSAQVQPVVALAFAPQLAGRALSAYNLVIFVGIFIVQWGIGLGADALESIGWRAAD
                     ALRGAMGVFCAISAACWLQFVWAGRR"
     gene            complement(2775..3329)
                     /locus_tag="PKV30_04405"
     CDS             complement(2775..3329)
                     /locus_tag="PKV30_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29495.1"
                     /translation="MVRRSRGYRPTLAAALAALGLLACTPSLNWRQVSLAPSSAIALL
                     PCKPDRTTSSVPLGGVPTELTVAGCDTGGATFALMAATLPAGRAADEVLAGWQQATLA
                     HMKAGDTPARLPFSPPGAAPLPHAQRVQAQGQRPGGGAVVAQAVWTARAVPGGGTELL
                     HAVLYSERPQPAAADAFFDGVRWP"
     gene            complement(3329..3724)
                     /locus_tag="PKV30_04410"
     CDS             complement(3329..3724)
                     /locus_tag="PKV30_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRL29496.1"
                     /translation="MSRDPQRLADYLAHILEAIERIDRYTRHMERDAFLGDSLVQDAV
                     IRNLEVIGEACSNIEKHCPAFAAGHPHLPLASAYQMRNALAHGYFKVDFDIVWQTIQH
                     DLPGLRAQVCAAAQAVSPFDDHGAGGSTT"
     gene            complement(3721..4011)
                     /locus_tag="PKV30_04415"
     CDS             complement(3721..4011)
                     /locus_tag="PKV30_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRL29497.1"
                     /translation="MKPSQALEMHRAAIRRVVESHRAANARVFGSVLLGQDTDSSDLD
                     LLVDPTPDTTLFDIGAMRHELGQLLGVPVDVLTPNALPDRFRARVLAQARPV"
     gene            4069..5304
                     /locus_tag="PKV30_04420"
     CDS             4069..5304
                     /locus_tag="PKV30_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRL29498.1"
                     /translation="MNSLEGIRVLDLSRVLAGPWCTQTLADLGADVIKIERPGAGDDT
                     RNWGPPFLPTADGRESHESAYYLGANRNKRSVTCDIAQPEGQALVRELAEHCDVFIEN
                     FKVGDMARYGLDYASLKAINARLVYCSLTGFGQSGPYAPRAGYDYIIQGMGGLMSVTG
                     ERDDLNGGPQKVGVAVADLFTGMYATVGILAALRHAERTGEGQHLDMALLDTQVAMLA
                     NLGANYLVSDQAPGRMGNAHQNIVPYQVFEVAPRADGGKDFIILAVGNDGQYAKFCEV
                     AGRPDLASEPRFVKNADRVRHRAELVPLLEAVMKTRPKADWLTALEAAKVPCGAINSL
                     DEVFADPQVQARGMVTHWQHPLRADLPLVSSPIKLEKTPVRTDRPPPMLGQHTDEVLG
                     ELLGVDAARLATLRDRQVI"
     gene            5519..6259
                     /locus_tag="PKV30_04425"
     CDS             5519..6259
                     /locus_tag="PKV30_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29499.1"
                     /translation="MAHYLLVHGAWGGAWYWRDTLSHLTRAGHRAHAVTLTGCGERAH
                     LLTPAIDLETHIADVLGALDAEEMQGCILVVHSYAGMLGTAVADRRPGQIAHLVYVDA
                     VLPAPGESWSSGQPAETRAARIASAERDPQYAFAAPDPTAAFGLTGPAADWVRRRQTP
                     HPGHTYTQPLDFDPARVAAVPRTFISCTAPRLPTIDVARARMADPAFWGGAWNGGGGV
                     RVVEMATGHDPMVSEPEAFNRLLLDVAA"
     gene            complement(6261..6623)
                     /locus_tag="PKV30_04430"
     CDS             complement(6261..6623)
                     /locus_tag="PKV30_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HRL29500.1"
                     /translation="MPLLANIAVAAVALLHVYILVLEMFLWTGPAGRKAFGLTPEFAE
                     QTRVLGANQGLYNGFLAAGLLFGLYLGGEQGFLFKAFFLTCVLVAGMYGAATASRKIL
                     FVQALPAAIALALLLLAR"
     gene            6755..7261
                     /locus_tag="PKV30_04435"
     CDS             6755..7261
                     /locus_tag="PKV30_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL29501.1"
                     /translation="MPRQSPIRRRALAGLTALALTAALAGCAQDAAPESTFVLLDGSR
                     QTTADMKGKVTLVNFWATSCTTCVAEMPQIVSTYDKYKAKGYDTLAVAMQYDPPSYVV
                     NFAETRKLPFKVAIDNTGKVAQAWGDVKLTPTTYIVNKQGQIVKKYVGAPNFDELHKL
                     IEKLLAEA"
     gene            complement(7488..7943)
                     /locus_tag="PKV30_04440"
     CDS             complement(7488..7943)
                     /locus_tag="PKV30_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRL29502.1"
                     /translation="MKKLLPTAALLLAATLAAPAHAQFAKAEDAIQYRQGAFRVLGHH
                     FGRLGAMANGKMPYNAATAAADGDVIAAVAKLPVTGFVPGSETGHDTRALPALWKELP
                     KVKELSDKMVAETGKLAVAAKSGNLDQLKAAFGPAAQTCKACHDDFRKK"
     gene            8074..8748
                     /locus_tag="PKV30_04445"
     CDS             8074..8748
                     /locus_tag="PKV30_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRL29503.1"
                     /translation="MNAVRIWDLPTRLFHWSLAICVIALVVTAKVGGDAMNWHFRLGY
                     AVFTLLLFRLLWGLVGGHWSRFASFFPTPGRLARYLGGRATAHDTAGHNPLGALSVLA
                     MLAVLSVQVGTGLFADDEIASAGPLTALVSGSVVSAATTWHKSWGQYLVIGLVVLHVL
                     AIVIYRLRRHDLVTPMLHGDKPLAGAVPAARDSAGTRLLALALLAVCAGVVYAVVQLG
                     HRPMLG"
     gene            8794..9291
                     /locus_tag="PKV30_04450"
     CDS             8794..9291
                     /locus_tag="PKV30_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL29504.1"
                     /translation="MDTRPPVDITLAQSAEQLEASRTLFREYAAQLGVDLGFQGFEDE
                     LAALPGDYGAPRGALLLASVDGELAGCCALRPIDDADYPNAAEMKRLFVRKAFRGFGL
                     GRLLAEAILTCAVERGYASVLLDTLDDMESARALYEDLGFEEIPPYYHNPLPGAHYLK
                     VDLPA"
     gene            complement(9380..9889)
                     /locus_tag="PKV30_04455"
     CDS             complement(9380..9889)
                     /locus_tag="PKV30_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRL29505.1"
                     /translation="MIKVGDTLPAVTLAEYSEVEGNGCSIGPNPVDIAKAAAGKTVAV
                     FAVPGAFTPTCSAKHVPSYLQNYDAMKAAGVDEIWCLSVNDPFVMGAWARDQKTDGKV
                     RMLADGDAAFAKATGLTLDLTGKGLGLRSNRYSMLVKDGKVATLNVEAPGKFEVSDGA
                     TLLAQAKGA"
     gene            complement(10029..>10276)
                     /gene="rpsQ"
                     /locus_tag="PKV30_04460"
     CDS             complement(10029..>10276)
                     /gene="rpsQ"
                     /locus_tag="PKV30_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRL29506.1"
                     /translation="KRTLIGKVVSDKRAKTVTVLVERRVKHPIYDKIMMRSSKYHAHD
                     ENGEYKMGDTIEITESRPLSKTKNWVVTRLVQKAAQV"
BASE COUNT         1713 a         3565 c         3390 g         1608 t
ORIGIN      
        1 tgacccagtc gtcctgccgc accagctggc tggccgagcg cgcgcccacc acggcgggga
       61 tgtccatgct gcgcgccacg atggcggtgt ggctggtctt gccgcccaca tcagtcacga
      121 agcccgcaaa cacgctcttc ttgaactgca gcatgtcggc cggcgacagg tcgtgcgcca
      181 ccagcaccag cggcacgtct tgcgtgtcgt ccaggcccag atcctgctgg cgcggtgcgc
      241 gtggcggcgg cgcggcgacc ggcgtggcgg cgcccttcag gtggcgcagc acgcgctcga
      301 cgacctgctc aagatcggcc ttgcgctcgc gcaggtactc gtcctccatc tcgtcgaact
      361 ggcgcaccac cacctcgagc tggctcacca gcgcccactc ggcgttgtac agccgctcct
      421 tgatccagcg gcggatctcg taggccatgc tctcgtcttc gagcagcatc aggtgcacgt
      481 cgagcatggc ggccagctcg ggcggagcgt ccttgggcat ggtggcctgc aggcgctgca
      541 gctcctcgat caccgcatcg cgcgccacgc gcaggcgctc gacttcggac ttgatctgat
      601 cggactcgat gaagtagtgc gccacatcga cgcggctgga cgccaccagc accgcgcggc
      661 cgagggcgat accgcgagcg acgggcaacc cgtggatggc gaaagtcatg cgcgtgctcc
      721 acgtgcgatg acggaatgac ctcgctgcgc ttcgatgaag gcgctgacgc gccatgacca
      781 gacggaccca gtcatttcgt catggccgcg cagcggcgaa ctcatttcgt cattccccct
      841 cgccgaactt gtcggcgatc agggccagca aggcatccat cgcctcctgc tcctgtggcc
      901 catcggtttc gacggtgacc acgctgccga tgccggcggc cagcatcatc acgcccatga
      961 tgctcttggc gttgacgcgg cgctcgccgc ggctcatcca cacctcgcac gggtagctgc
     1021 cggccagctt ggtgagcttg gccgaggccc gcgcgtgcag gccgagctta ttggtgatgg
     1081 tcgtgtcggt cttgatcatg cgggactttt cggctctggt tctgcggcgc ggcgctggcc
     1141 accggcatca cgccctgcac gccaccggcc agcgcgcgct gggccatcgc atccagcgac
     1201 tcggcgcggt agctgacggc gcgcagcagc atcggcaggt tgacgcctgc cagcagccgc
     1261 acgcgatcga cctcgcacag gcgctgcgcg acgttgcaag gcgtggcgcc gaacacgtcg
     1321 ctcagcacca gcacggggcg ctcgcccagc tgcgcaatca gcgcctcggc ggcggccagg
     1381 gtttgctcgg gcggcgcgtc aggatcgacg tcgagcgcgg cgatgtcggc cgccgcgtcg
     1441 ggaaacacat gcagcgcccc ctggcgcagt gcgtttgcca gcggggcatg ggcgatcagc
     1501 agcacggcgt tcatggccgc attatcggcg cccggcccac acgaactgca gccagcacgc
     1561 ggcgctgatg gcgcagaaca cgcccatcgc gccgcgcagc gcgtcggccg cccgccagcc
     1621 gatggactcc agcgcatcgg cacccaggcc gatgccccac tgcacgatga agatgccgac
     1681 gaagatgacc aggttgtagg ccgacagcgc gcgccccgcc agctgcggcg caaacgccag
     1741 cgccaccacc ggctgcacct gcgccgacgg cgtgctcagc atgcaataca gcgccagcag
     1801 cacaaagctg aagccgcccg ccgccggccc ccacgccacc agcgccgcca gcacgacgaa
     1861 gctgaacggc tgcacgcgtg ccaccatgcg gtcaacgctg atgccgcgcg ccgccatgcg
     1921 cggcagcaac agcccccagc tccagaacgt gaccagcatg cccaggttga tccagaacag
     1981 cccggtggcc gcctgcagcg gcgtggcgcc ggccacgcgc gtcagccacg gcccggccca
     2041 cagcgactgg atggcgacca tgccgccaaa gctcaccatg cccagcggcg aagcgcgcct
     2101 gaaatagggg ttgcgccaga tctctgaata tgaacctttt tggccgttgg cgcttgtctg
     2161 atctgcgcca gcagctatgt tttgcggagc aaccgcgcgc cagcgcggca ccgcccaggc
     2221 gatcaccgcc atcgccagca gcaccagcgc ggccaggccc cagaacagcg cgcgccagcc
     2281 caccaacggc agcagccact gcaccggcag cgtcgaggcc agcatgccga gcgagccggt
     2341 catcagcatc cacgaattgg cgcgcagctg cgtgccgggg tcgaaccagc ggcggtagcc
     2401 ggtcagcggc gccatcaggc acgctgccac gcccacgccc atcaacagcc gcgccgccat
     2461 caggccgctg aagccgccgg ccagcgcaaa ggcgacgcaa cccagcaccg ccacgctgag
     2521 caggccgagg atgaccttct tcggcccatg ccgatccagc caggcgccca gcggcaactg
     2581 caccgccgca aagcccagaa agtagccgcc cgccagcaac cccagatcgc gcgcatgcag
     2641 gctgaattcg gccgtcaaca ctggcgacag cgtggcggtg accgcgcgca gcagcgccga
     2701 caagaagtag gcgccggcaa aggccagaaa gatgatcacc gccgtgcggc gcggcagcgg
     2761 ctccggcggc gcgttcatgg ccagcggacg ccgtcgaaga aggcgtcggc cgccgccggc
     2821 tgcgggcgct cgctgtacag cacggcgtgc aacaactcgg tgccgccgcc gggcacggcg
     2881 cgcgccgtcc acaccgcctg cgccaccacc gcgccgcccc cggggcgctg gccctgcgcc
     2941 tgcacgcgct gcgcgtgcgg cagcggcgcg gcgccgggcg ggctgaaagg caaacgcgcc
     3001 ggcgtgtcgc cggccttcat gtgggccagc gtggcctgct gccagccggc cagcacctcg
     3061 tcggccgctc ggccggccgg cagcgtggcg gccatcaggg caaaggtagc gccgccggta
     3121 tcgcaaccgg ccaccgtcaa ttcggtcggc acgccgccca gcggcacgct gctcgtggtg
     3181 cggtcgggct tgcacggcag cagcgcgatg gcgctggacg gcgccagact cacctgacgc
     3241 cagttgagtg atggggtgca ggccagcaaa cccagcgccg ccagcgcagc ggcgagagtc
     3301 ggccgatagc cacgcgaacg gcgtaccatc acgtcgtgga accgcctgcg ccgtgatcgt
     3361 caaacggcga cacggcctgc gccgccgcgc agacttgcgc acgcaagccg ggcaagtcgt
     3421 gctgaatagt ctgccagacg atgtcgaagt ccaccttgaa atagccgtgc gccagcgcat
     3481 tgcgcatctg ataggcggac gccaacggaa gatgcggatg acccgccgca aacgccgggc
     3541 aatgcttctc gatattgctg caggcctcgc cgataacctc caggttgcgg atcacggcat
     3601 cctgcaccaa cgaatcgccg aggaaggcgt cacgctccat gtgccgcgtg taacggtcga
     3661 tgcgctcgat ggcttcgagg atgtgcgcca ggtagtcggc cagtcgctgc ggatcgcggc
     3721 tcataccggc cgagcttggg ccagcacccg ggcgcgaaag cggtcgggca gcgcgttggg
     3781 cgtcaacaca tcgaccggca cgcccagcaa ctggcccaac tcgtgccgca tcgcgccgat
     3841 gtcaaacagc gtcgtgtccg gcgtcggatc aaccagcagg tcgaggtcgc tgctgtccgt
     3901 gtcctgccca agcagcaccg agccgaacac gcgtgcattg gcggcacggt gcgactcgac
     3961 aacgcggcga atggccgcgc ggtgcatttc caaggcttgc gagggcttca taatgggtga
     4021 gagggttcag ctttcatggt aacgcaagcg atgcgccaga atgccgccat gaactcgctc
     4081 gaaggcatcc gcgtactcga cctctcccgc gtgctggccg gcccgtggtg cacgcaaacc
     4141 ttggccgacc tgggcgctga cgtgatcaag atcgagcgcc cgggcgcggg cgacgacacg
     4201 cgcaattggg gcccgccctt tctgcccacg gccgatggcc gcgaatcgca cgaatcggcg
     4261 tattacctgg gcgccaaccg caacaagcgc agcgttacct gcgacatcgc ccagcctgag
     4321 ggccaggcgc tggtgcgcga actggccgag cactgcgacg tgttcatcga gaacttcaag
     4381 gtcggcgaca tggcgcgcta cgggctcgat tacgccagcc tgaaggccat caacgcgcgc
     4441 ctggtgtatt gcagcctgac gggttttggc caaagcggcc cctatgcgcc gcgcgcgggc
     4501 tacgactaca tcatccaggg catgggcggc ctgatgagcg tgaccggcga gcgcgacgat
     4561 ttgaatggcg gcccgcaaaa agtcggtgtg gcggtggccg atctgttcac cggcatgtac
     4621 gccacggtgg gcatcctggc cgcactgcgc cacgccgagc gcacgggcga aggccagcat
     4681 ttggacatgg cgctgctcga cacgcaggtt gccatgctgg ccaacctggg cgccaactat
     4741 ctggtcagcg accaggcgcc cggccgcatg ggcaacgcgc accagaacat cgtgccctac
     4801 caggtgttcg aggtggcgcc gcgcgcggat gggggcaagg atttcatcat tctggccgtg
     4861 ggcaacgacg gccagtacgc caagttctgc gaagtcgccg gtcggcccga cctggccagc
     4921 gagccgcgct ttgtgaagaa tgccgaccgc gtgcgccacc gcgccgagct ggtgccgctg
     4981 ctggaggcgg tgatgaaaac gcgccccaag gccgactggc tgaccgcgct ggaagccgcc
     5041 aaggtgccct gcggcgcgat caacagcctg gacgaagtgt ttgccgaccc gcaggtgcag
     5101 gcgcgcggca tggtcacgca ctggcagcac ccgctgcggg ctgatttgcc gctggtgagc
     5161 agccccatca agctcgaaaa gaccccggtg cgcaccgacc gcccgccgcc catgctgggc
     5221 cagcacaccg acgaggtgct gggcgaactg ctgggtgtgg atgccgcgcg gctggccacg
     5281 ctgcgcgatc gccaggtgat ttgattgcta tgctaaaagt agcttcttgc gcttggcctg
     5341 cctgcgccag tggcactttt aattcagaat accggacgca gaggacgcaa aggattcgca
     5401 gagaacgcaa aaaatacaaa cagaaaatct tcaaggtttt cttctgcgtc ctctgcgaaa
     5461 tctctgtgcc ctctgcgtcc ggctgttcgg cggcttaacc ctgaccaagg agacccccat
     5521 ggcccattac ctgctcgttc acggcgcctg gggcggcgcg tggtactggc gtgacacgct
     5581 tagccacctg acccgtgccg gccaccgcgc gcacgccgtc acgctcaccg gctgcggcga
     5641 acgcgcccac ctgctcacgc ccgcgatcga cctggaaacg cacatcgccg acgtgctggg
     5701 cgcgctggat gccgaagaga tgcaaggctg catcctcgtc gtgcacagct acgccggcat
     5761 gctcggcacc gccgtggccg accggcggcc ggggcagatc gcgcacctgg tctacgtcga
     5821 cgccgtgctg cccgcgcccg gcgaaagctg gagcagcggt cagccggccg agacccgcgc
     5881 cgcgcgcatc gccagcgcgg agcgcgatcc gcaatacgcc tttgccgcgc ccgaccccac
     5941 ggccgccttt ggcctcaccg gccccgccgc cgactgggtg cgccgccgcc agacgccgca
     6001 cccgggccac acctacacgc agccgctcga tttcgacccg gcgcgcgtgg ccgccgtgcc
     6061 gcgcaccttc atcagctgca ccgcgccgcg tctgccgacc atcgacgtcg cgcgcgcgcg
     6121 catggccgat cccgctttct ggggcggcgc gtggaacggt ggcggcggcg tgcgcgtggt
     6181 cgaaatggcg accgggcacg atccgatggt cagcgagccg gaagccttca accggctgct
     6241 gctggacgtg gcggcctgac ttaccgcgcc agcaacagca gcgccagcgc gatggccgcc
     6301 ggcagcgcct gcacgaacag gatcttgcgg ctcgccgtgg ccgcgccgta catgccagcg
     6361 accagcacgc aggtcaggaa aaacgccttg aacagaaaac cttgttcacc gcccaggtac
     6421 agcccgaaca gcaggccggc ggccagaaag ccgttgtata ggccctgatt ggcgcccagc
     6481 acccgcgtct gctcggcaaa ctccggcgtc agcccgaacg ccttgcggcc ggcgggcccg
     6541 gtccacaaga acatctccag caccaggatg tagacgtgca gcagcgcgac ggcggccacg
     6601 gcgatgttgg cgagaagcgg catgggacgt ttcttgaaag tgatcggcca atttaccgca
     6661 cacgggccag cccagaactt tccgcgcgcc accgagtcca ccctttgccc ttgcgacagc
     6721 accagcgcgt gccaaagcct agaattttgc cgtgatgccc cgccaatccc ccatccgccg
     6781 ccgcgccctc gccggcctga ccgcactggc actgaccgct gcgctggccg gctgcgcgca
     6841 agacgccgcg cccgaatcca ccttcgtcct gctcgacggc tcacgccaga ccaccgccga
     6901 catgaagggc aaggtcacgc tggtcaactt ctgggccacc agctgcacca cctgcgtggc
     6961 cgagatgccg cagatcgtct ccacttacga caagtacaag gccaagggct acgacacgct
     7021 ggccgtggcg atgcagtacg acccgcccag ctacgtggtc aactttgccg aaacgcgcaa
     7081 gctgcccttc aaggtcgcca tcgacaacac cggcaaggtg gcccaggctt ggggcgacgt
     7141 gaagctgacg cccaccacct acatcgtcaa caagcagggc cagatcgtca aaaaatacgt
     7201 cggcgcgccc aacttcgacg agttgcacaa gctgatcgag aagctgctgg ccgaggcctg
     7261 aagccagcgc cccataaaaa agccgcacca aggtgcggct tttttaatgg tctttttagg
     7321 ctccagcgct tgtctgaaaa gcgcgagcag ctcatatttt cggagcgatc gaccgtgaca
     7381 cagcccgcgc gggcacccgc ggaagggctt ggcccgcccg ctgggtgcgt cccccttccc
     7441 gcgaagcgag agaaggggga agcggcgtca gccgctcagg gggttggtca ctttttcctg
     7501 aagtcgtcat ggcacgcctt gcacgtctgc gcggcggggc caaaggcggc tttcagctga
     7561 tccaggttgc cgctcttggc ggccacggcc agcttgccgg tttcggcgac catcttgtcg
     7621 ctcaattcct tcaccttggg cagttctttc cacagcgccg gcagcgcgcg cgtgtcgtgg
     7681 ccggtttcgg aacccggcac gaagccggtc accggcagct tggcgacggc ggcaatcacg
     7741 tcgccgtcgg ctgcggcggt ggcggcgttg tagggcatct tgccgttggc catggcgccc
     7801 aggcggccga agtggtggcc gagcacgcgg aacgcgccct ggcggtactg aatggcgtct
     7861 tcggccttgg cgaactgggc gtgcgccggc gcggccaggg tggcggcaag cagcagggcg
     7921 gcggtgggaa gcagtttctt catttttcgg aggctcctgt ggggtggtgg ctgcgggcga
     7981 gcgcgtgcgt tgcgcacgca aacgtcatta aactcgccag acgccgggcg cagcatagcg
     8041 tctttcctca tttcaagcgg ggtgcctttc gtcatgaacg ccgttcgcat ctgggacttg
     8101 ccgacccgcc tcttccactg gtcactggcg atctgcgtga tcgcgctggt cgtcaccgcc
     8161 aaggtcggcg gcgacgccat gaactggcac tttcgcctgg gctacgcagt gttcacgctg
     8221 cttttgttcc ggttgctgtg gggcttggtc ggcggccact ggtcgcgctt tgccagcttc
     8281 ttcccgacgc cggggcggct ggcgcgctac ctgggcgggc gcgccacggc gcacgacacg
     8341 gcgggccaca acccgctggg cgcgctgtcg gtgctggcca tgctggcggt gctgtcggtg
     8401 caggtcggca cgggcctgtt tgccgatgac gaaattgcct ccgccgggcc gctgacggcg
     8461 ctggtgtccg gcagcgtggt gtcggcggcg accacctggc acaagtcttg ggggcaatac
     8521 ctcgtaatcg ggctggtggt gctgcatgtg ctggccatcg tcatctaccg gctgcggcgg
     8581 cacgacctgg tcacgccgat gctgcacggc gacaagccac tggcgggcgc cgtgccagcg
     8641 gcgcgcgaca gcgccggcac gcgcctgctg gcgctggcgc tgctggccgt ttgcgccggc
     8701 gtggtttacg cggtggtgca actcggccac cggccgatgc tgggatagac tggcctggcg
     8761 cgcccacgcc ggcgcggctt tctgatcaca tccatggaca cgcgcccccc cgtcgatatc
     8821 accctggcgc agagcgccga gcaattggaa gcgtcacgca cgctgtttcg cgaatacgcg
     8881 gcgcaattgg gcgtcgacct gggttttcag ggctttgagg acgagttggc cgcactgccg
     8941 ggcgactacg gtgcgccgcg cggtgcactg ctgctggcct cggtcgatgg cgaactggcc
     9001 ggctgctgcg cgctgcgccc gatcgacgat gccgactacc ccaacgccgc cgagatgaag
     9061 cgcctgttcg tgcgcaaggc gtttcgcggc ttcggcctcg ggcgcctgct ggccgaggcg
     9121 atcctgacct gcgccgtcga gcgtggctac gcgtcggtac tgctcgatac gctggacgac
     9181 atggaatcgg cgcgcgcgct gtacgaggat ctgggctttg aggaaatccc gccctactac
     9241 cacaacccgc tgccgggggc gcactacctg aaggtggatt tgcccgcttg agcggcgccg
     9301 cagcttatcg agaaaactat caacttcata gctgctggcg cttgtcagac aagcgccagc
     9361 agccgatttg gcatcaaact caggcgccct tggcctgtgc cagcagggtg gcgccgtcgc
     9421 tcacctcgaa cttgcccggc gcctcgacgt tcagcgtcgc caccttgccg tccttgacca
     9481 gcatcgaata gcggttgctg cgcaggccca ggcccttgcc ggtcagatcg agcgtcaggc
     9541 cggtggcctt ggcgaaggcg gcgtcgccgt cggccagcat gcgcaccttg ccgtcggtct
     9601 tctggtcgcg cgcccaggcg cccatgacga aggggtcgtt gaccgacagg caccaaatct
     9661 catccacgcc cgctgccttc atggcgtcgt agttctgcag gtagctcggc acgtgcttgg
     9721 ccgagcaagt gggcgtgaag gcgcccggga cggcaaacac ggccaccgtc ttgccggcgg
     9781 ctgccttggc gatgtcgacc gggttagggc cgatgctgca gccgttgccc tcgacttcgg
     9841 aatattcggc cagggtgacg gcgggcaggg tgtctccgac tttgatcatc aatgtgctcc
     9901 ttgggggttg tggacgaaac gcaggccatc aaaaagccga cccacaaatg aaaacagccc
     9961 acgcattgtg ggctgttggc aggcaatgtg ctgacggcgc cggtttgggc gctggcaccg
    10021 cgtgggcttt acacctgcgc ggccttctgg accaggcggg tgacaaccca gttcttggtc
    10081 ttggacagcg gacggctctc ggtgatctcg atggtgtcgc ccatcttgta ttcaccgttt
    10141 tcgtcgtgcg cgtggtactt ggacgaacgc atcatgatct tgtcgtagat cgggtgcttg
    10201 acgcgacgct cgaccagcac ggtcacggtc ttggcgcgct tgtcgctgac gaccttgccg
    10261 atcagggtgc gcttga
//
LOCUS       DAOMVO010000056        10118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1632_length_10118_cov_14.754745, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000056 DAOMVO010000000
VERSION     DAOMVO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_10118_cov_14.754745"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKV30_04465"
     CDS             <1..1205
                     /locus_tag="PKV30_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRL29507.1"
                     /translation="DAHGKKMSKSEGNVLDPVDLIDGIALEPLLQKRTTGLRKPETAP
                     KVRENTKKEFPDGIPGYGADALRFTFAAMATLGRTVNFDSKRCEGYRNFCNKLWNASR
                     FVLMNCEGYDCDSSGDSSDARSTADRWIVSRLQKTEGQVEKGFADYRLDTVANTLYDF
                     VWNEYCDWYLEIAKVQIQTGTPAQQRATRRTLVRVLEAILRLAHPIIPFVTEALWQAV
                     APVAQKTGASIAVAPYPQAQAANIDEAAEAEMARFKQLVDACRNLRGEMNVSPAQRLP
                     LFALGDAGFIRAHAPALQALAKLSEVKVFDAEANWAAAAQAAPVAVVGDARLALFMEV
                     DVAAEKARLAKEAGRLEGEIAKANGKLSNQAFVAKAPPAVIDQEKKRVADFTAALDKL
                     RDQLQRLG"
     gene            complement(1387..2352)
                     /locus_tag="PKV30_04470"
     CDS             complement(1387..2352)
                     /locus_tag="PKV30_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL29508.1"
                     /translation="MDILLLEDLAADARQWLAARHQVDYRPELLEDPARLHAQLQQVD
                     ALVAPPRLKINGQLLDSAPQLVAVGRIHDGTENIDFEACQKRRVRVIQASSATVRASA
                     EHQLLALLQLFRSGLRPPTGLPGARGLPGREINDSVIALFGMSPPAHVLATMLVPLGV
                     RVVGYDPAVHRNADLWRRLGVQPMAMNEMLEIADAVAMQTIYASRYRGLVGERVLASC
                     KPGQVWTSISRPSLFDLDALADAVRSGRIGAFTMDSDDERLHASDSPLRELPHLQITP
                     RLAPFTQESLVRGSWYLVDRIHETLMLAEIRSQTGPQDSEPMPLA"
     gene            2466..3353
                     /gene="galU"
                     /locus_tag="PKV30_04475"
     CDS             2466..3353
                     /gene="galU"
                     /locus_tag="PKV30_04475"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRL29509.1"
                     /translation="MPASRQVLTAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYA
                     VEEAYAAGVRHMVFVTGRSKRAIEDHFDTAYELESEMEAAGKRDLLKVVRAVAPDDMD
                     CTYVRQPRSLGLGHAVLCAAHIVGEQPFAVLLADDLMVGPPGGLPVLAQMTEAFASLG
                     HSLLAVQEVPADQVRRYGIVAGTPAGERLIQLERIVEKPAPEAAPSRMGVAGRYILTP
                     RIFDEIRQQTPGAGGEIQLTDAIARLMSKEAVYAYQYSGKRYDCGSKEGFLQATVELA
                     LQHPQMGAPFRDYLKALSL"
     gene            complement(3355..4209)
                     /locus_tag="PKV30_04480"
     CDS             complement(3355..4209)
                     /locus_tag="PKV30_04480"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRL29510.1"
                     /translation="MYDTLISAGQLQQALASATPPVVLDCSFDLTDGAAGQRAHDAAH
                     IPGAFYVHLDRTLSGAKTGRNGRHPLPGQEDFVQAMAALGLDNDTQVVAYDNAGGMYA
                     ARLWWMLRWVGHAAVAVLDGGLAAWKAAGQPLQTGATAPRARGDFSLRPALVSCVDYD
                     AVKANIDSRERLVVDARAPDRFRGENETLDAIGGHIPGARNRLFRDNLAADGRFKPAA
                     QLRQEFDAITGGRPASELINQCGSGVTACHNLLAMEVAGLRGAALYPGSWSEWSAQPG
                     APIATGAD"
     gene            complement(4222..4449)
                     /locus_tag="PKV30_04485"
     CDS             complement(4222..4449)
                     /locus_tag="PKV30_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRL29511.1"
                     /translation="MDIVKEVDARGLNCPLPILKAKKALAELQSGELLKVLSTDGGAL
                     RDFQAFAKQTGNELVEQTTEGDTTAHVLRRR"
     gene            complement(4616..5164)
                     /locus_tag="PKV30_04490"
     CDS             complement(4616..5164)
                     /locus_tag="PKV30_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRL29512.1"
                     /translation="MPRWNFCPQCANPLEMLPMQEDSGIVERLRCAACGFTHWNNPTP
                     VLAAIVEYRGQVLLARNAAWPAKMFALITGFMEAGETPQEGIAREVKEETNLDVQSLG
                     LVGVYDFQRMNQVIIAYHAVAEGELKLSPELVDWRLYDLPDLKCWPAGTGYALADWLR
                     TRGHEPVFFTPEENAERRRGLD"
     gene            complement(5230..6132)
                     /gene="cysM"
                     /locus_tag="PKV30_04495"
     CDS             complement(5230..6132)
                     /gene="cysM"
                     /locus_tag="PKV30_04495"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HRL29513.1"
                     /translation="MNYPTIEDAVGRTPLVRLQRLDAERNAARGNVVLAKLEGNNPAG
                     SVKDRPALSMIRRAEERGEIKPGDTLIEATSGNTGIALAMAAAIKGYHMVLIMPEDLS
                     VERAQTMKAFGAELILTPRSGGMEYARDLADKMVAQGKGRVLDQFANPDNPRIHYETT
                     GPELWEQTDGRITHFVSAMGTTGTITGVSRFLKEKNPKVQIIGAQPAEGSRIPGIRKW
                     PEEYLPKIYDPKAVDELVLVDQLDAEDMARRLAREEGIFGGISSGGAGWVAQQIAARE
                     QGATIAFVVCDRGDRYLSTGVFPA"
     gene            complement(6741..6983)
                     /locus_tag="PKV30_04500"
     CDS             complement(6741..6983)
                     /locus_tag="PKV30_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HRL29514.1"
                     /translation="MSTTKKLRLGPLPRTESVKLTFACPVSLKADLDRYAALHAQVYG
                     EAVDTATLIPYMLEAFMEGDRGFKRTQTKNEKATPA"
     gene            complement(6980..7177)
                     /locus_tag="PKV30_04505"
                     /pseudo
     CDS             complement(6980..7177)
                     /locus_tag="PKV30_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
     gene            complement(7512..8090)
                     /locus_tag="PKV30_04510"
                     /pseudo
     CDS             complement(7512..8090)
                     /locus_tag="PKV30_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
     gene            complement(8295..8831)
                     /locus_tag="PKV30_04515"
     CDS             complement(8295..8831)
                     /locus_tag="PKV30_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29515.1"
                     /translation="MKALPSRDGSLIRGKLSHIQHSEHPTMVSIRHIVTQWGRRIVGG
                     LIVALLFSQMALAMYACPKLDADTHAASMQMAGMAMDEATGMSAMPDCHAMPGTMDDE
                     SPQLCRAHCSGDGKPAPSPQGLDGLSTAAAHAVWTAYLLPVVPDPQSSTDARADPAWL
                     DHRTGSFPFHLTFQVLRN"
     gene            complement(8875..9132)
                     /locus_tag="PKV30_04520"
     CDS             complement(8875..9132)
                     /locus_tag="PKV30_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29516.1"
                     /translation="MNGMYMNDMGWWLGAHWLTMLLGAVVIVLPFWKTFAKAGFSGWL
                     GLLMIVPMVNLIALYVLAFSDWPRLRRADKLDSGEMNPGIR"
     gene            complement(9171..>10118)
                     /locus_tag="PKV30_04525"
     CDS             complement(9171..>10118)
                     /locus_tag="PKV30_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRL29517.1"
                     /translation="LIVDKTGTLTEGRPAFDTAVAAPGYTADEVLRLAASLDQGSEHP
                     LADAIVSAAREKGLQLAKPTDFESGSGIGVRGTVEGRRLALGNTALMEQEGLDVSALK
                     ADAERLRGEGASVMHLAVDGRLAGLLAVTDPIKSSTPEAIHTLHAGGLRIVMATGDGL
                     TTARAVGTRLAIDEVHGEVKPADKLALVEKFQRAGHVVAMAGDGINDAPALAKADVGV
                     AMGTGTDVAMNSGQVTLVKGDLRGIAAARQISLDTVRNMRQNLLFAFVYNGIGVPIAA
                     GVLYPITGWLLSPMVAALAMSLSSASVIFNALRLRRARG"
BASE COUNT         1706 a         3395 c         3279 g         1738 t
ORIGIN      
        1 tcgacgcgca cggcaagaag atgagcaaga gcgaaggcaa cgtgctcgac ccggtcgatt
       61 tgatcgacgg cattgcgctg gagccgctcc tgcaaaagcg caccaccggc ctgcgcaagc
      121 ccgagacggc gccgaaggtg cgcgaaaaca ccaagaagga attccccgac ggcatcccgg
      181 gctatggcgc cgacgcactg cgcttcacgt ttgccgccat ggccacgctg gggcgcaccg
      241 tcaacttcga cagcaagcgc tgcgagggct accgcaactt ctgcaacaag ctgtggaacg
      301 ccagccgctt cgtgctgatg aattgcgagg gctacgattg tgatagctcc ggcgacagct
      361 ctgacgcgcg ctccaccgcc gaccgctgga tcgtcagtcg cctgcaaaag actgaaggcc
      421 aggtcgaaaa aggctttgcc gactaccgcc tggacaccgt ggccaacacg ctgtacgact
      481 tcgtctggaa cgagtattgc gactggtatc tggagatcgc caaggtgcag atccagaccg
      541 gcacgcccgc gcagcagcgc gccacgcgcc gcacgctggt gcgcgtgctg gaagccatcc
      601 tgcgcctggc gcacccgatc attccgttcg tgaccgaggc gctgtggcag gccgtggcgc
      661 ccgtggcgca aaagaccggc gcttcgattg ctgtagcgcc ctacccgcag gcgcaggccg
      721 ccaacatcga cgaggcggcc gaagccgaga tggcgcgctt caagcagttg gtggatgcct
      781 gtcgcaacct gcgcggcgaa atgaacgtct cgcccgcgca gcggcttccg ttgtttgcgc
      841 tgggcgatgc cggcttcatc cgcgcccacg cgcctgcgct gcaggccttg gccaagctga
      901 gtgaagtgaa ggtgttcgac gccgaagcca actgggccgc cgctgcccag gcggcgccag
      961 tggccgtggt gggcgacgcg cggctggcgc tgttcatgga ggtggacgtg gcggccgaga
     1021 aggcgcgcct ggccaaggaa gccggccggc tggagggcga aattgcaaaa gccaacggca
     1081 agctgagcaa ccaggccttt gtcgccaagg cgccgccggc cgtgatcgac caggagaaaa
     1141 agcgcgtggc cgacttcacc gccgcgctgg acaagctgcg cgatcagctg cagcgactgg
     1201 gttgatagag tgaaacccag caatgctcca acaccgttcg tcctgagcct gtcgaaggtc
     1261 tgggtatgtg agacacaggc ttcgacagga tcagcccgaa cgggtgattg gtcgtcgaac
     1321 catctgcgag tcaaactata aaaacaatag ctgctcgcgc tgactacgcc aagaccagcg
     1381 gcgttttcaa gccaacggca tcggctcgct gtcctgcggc ccggtctggc ttctgatctc
     1441 cgccagcatc agcgtttcgt gaatgcggtc gaccaggtac cagctgccac gcaccagtga
     1501 ctcctgcgtg aacggcgcca ggcgcggcgt gatctgcaag tgcggcaact cgcgcaaggg
     1561 cgaatcagag gcgtgcaggc gctcatcgtc gctgtccatc gtgaaggcac cgatgcggcc
     1621 cgagcgcacg gcgtcggcca gcgcatcgag gtcgaacagc gacggccggc tgatgctggt
     1681 ccacacctgc cccggcttgc aactggccag cacgcgctcg cccaccagcc cgcggtagcg
     1741 cgaggcgtag atggtctgca tggccaccgc gtcggcgatt tccagcatct cgttcatcgc
     1801 catcggctgc acgcccaggc gccgccaaag atcggcgttg cggtgcaccg ccgggtcgta
     1861 gccgaccacg cgcaccccca gcggcaccaa catggtggcc agcacgtgcg ccggtggcga
     1921 catgccgaac agggcgatca cgctgtcgtt gatctcgcgc cccggcaggc cgcgcgcgcc
     1981 cggcaggcca gtcggcggcc gcaggcccga acgaaacagc tgcaacaacg ccagcaactg
     2041 gtgctcggca ctggcgcgca cggtggcgct cgacgcctgg atcacgcgca cgcggcgctt
     2101 ctggcacgct tcgaaatcga tgttttcggt gccgtcgtgg atgcggccaa ccgccaccag
     2161 ctgcggcgcg ctgtcgagca actggccgtt gatcttgagc cggggcggcg ccaccagcgc
     2221 atcgacctgt tgcaactgcg cgtgcagccg cgccgggtct tccagcagct ctggccgata
     2281 atcgacctga tgccgtgccg ccagccactg gcgcgcatcg gcggccaaat cctccagcag
     2341 gagaatgtcc aagctcatgc ctccgttgat ccacttgttg tccggccgct cccggtgcca
     2401 cggcacagga gccgacggct gagcgctgct ttcttcggca ctttaccacc tcgctttttt
     2461 tctccatgcc cgcttcgcgc caggttctca ccgccgtttt tcccgtggcc ggcctcggca
     2521 cgcgctttct gcctgccacc aaggcaagcc cgaaggaaat gctgcccatc gtcgacaagc
     2581 cgctgatcca gtacgccgtg gaagaggcct acgccgccgg cgtgcgccac atggtgtttg
     2641 tcaccggccg cagcaagcgc gccatcgaag accatttcga caccgcctac gaactggaaa
     2701 gcgagatgga ggccgccggc aagcgcgatc tgctgaaggt ggtgcgcgcc gtggcgcccg
     2761 acgacatgga ctgcacctac gtgcgccagc ctcgctcgct gggcctcggc cacgccgtgc
     2821 tgtgcgccgc gcacatcgtg ggcgagcagc cctttgcggt gctgctggcg gatgacctga
     2881 tggtcgggcc gcccggcggg ctgccggtgc tggcgcagat gaccgaggcg tttgcctcgc
     2941 tcggccattc gctgctggcg gtgcaggaag tgccggccga ccaggtgcgc cgctacggca
     3001 tcgtggccgg tacgccagcc ggcgagcgct tgattcaact tgagcgcatc gtcgaaaaac
     3061 ccgcgcccga agccgcgccc tcgcgcatgg gcgtggctgg ccgctacatc ctcacgccgc
     3121 gcatcttcga tgaaatccgc cagcagacgc ccggcgccgg cggcgagatt cagctcaccg
     3181 atgcgattgc gcgcttgatg agcaaagagg ccgtctacgc ttaccaatac agcggcaagc
     3241 gctacgattg tgggagcaaa gagggcttcc tgcaggccac cgtggagctg gccttgcagc
     3301 acccgcagat gggtgcgccg tttcgcgact acctgaaagc gctcagcctg taggtcagtc
     3361 ggcgccggtc gcgatcggcg cgccgggctg cgcgctccac tcgctccacg agccggggta
     3421 cagcgcggca ccacgcaggc ccgccacctc catcgccagc aggttgtgac aggccgtgac
     3481 gcccgagccg cattggttga tcagttcgct ggccgggcgg ccgcccgtga tcgcatcgaa
     3541 ctcctgccgc aactgcgctg cgggcttgaa gcggccatcc gccgccaggt tgtcgcggaa
     3601 cagccggttg cgggcgcccg gaatgtggcc gccgatggcg tccaacgttt cgttctcgcc
     3661 gcgaaagcga tccggcgcgc gcgcatccac caccaggcgc tcgcgtgaat cgatattggc
     3721 cttcaccgcg tcgtaatcca cgcagctgac cagcgccggg cgcaggctga aatccccgcg
     3781 cgcgcgtggc gcagtcgccc cggtctgcag cggctgccca gccgccttcc acgccgccag
     3841 gccgccgtcc agcacggcca ccgcggcatg gccgacccag cgcagcatcc accacaggcg
     3901 ggcggcgtac atgccgccgg cgttgtcgta ggccaccacc tgcgtgtcgt tgtcgaggcc
     3961 cagcgccgcc atggcttgca cgaagtcttc ctggccaggc aaggggtgac gcccgttgcg
     4021 gccggtcttg gcgccgctca gcgtgcggtc caggtgcacg taaaacgcgc cggggatgtg
     4081 agccgcgtca tgggcacgct ggccggccgc gccatccgtc agatcgaagc tgcagtccag
     4141 caccacgggc ggcgtggcgg acgcgagcgc ttgctgcagt tggcccgctg aaatcagtgt
     4201 gtcgtaaatc atcgtgcgcc cctatcgccg gcgcagcaca tgcgccgtgg tgtcgccttc
     4261 ggtcgtctgc tcgaccagct cattgcccgt ctgcttggca aaggcctgaa agtcgcgcag
     4321 cgcgccgccg tcggttgaca gtactttcag caactcgccg ctttgcagct cggccaatgc
     4381 cttcttggct ttcagaatcg gcagcgggca gttcagcccg cgtgcgtcga cttccttcac
     4441 gatgtccatg gttcgatcct ggtcaggtgt tgatggcgct gcgcgccgcc cgcattgcat
     4501 gccacataaa gagtccaacg gctgcggagc aagccacgcg ggaacgccgt ggaacccggc
     4561 cacgggcgtt gtccccctgg gggaaggcgc cgaaggcgac tcagggggag tcatttcaat
     4621 ccagtccgcg ccggcgctct gcgttttctt ctggcgtgaa gaacaccggc tcgtgcccgc
     4681 gcgtgcgcaa ccagtcggcc agcgcatagc cggtgccggc cggccagcac ttcaggtcgg
     4741 gtaggtcgta caggcgccag tcgaccagct cgggcgacag tttcagttcg ccctccgcca
     4801 cggcgtggta ggcgatgatg acctggttca tgcgctggaa gtcgtacacg ccgaccagcc
     4861 cgagcgactg cacgtcaagg ttggtttctt ccttcacctc gcgcgcgatg ccttcttgcg
     4921 gcgtttcgcc cgcttccatg aaaccggtga tgagcgcgaa catcttggcc ggccacgcgg
     4981 cgttgcgcgc cagcagcacc tggccgcggt attcgacgat ggcggccagc accggcgtcg
     5041 ggttgttcca gtgcgtgaag ccgcaggcgg cgcagcgcaa gcgctcgacg atgccgctgt
     5101 cttcctgcat cggcagcatt tccagcgggt tcgcacattg ggggcagaaa ttccagcgcg
     5161 gcatatgctt ttgttttgat agcttctcgc gcctgtcagt caagcgccag cggccgtttc
     5221 attgaaatat caggccggaa acacgcctgt cgacagatag cggtcgccgc ggtcgcacac
     5281 cacaaacgcg atggtcgcgc cctgctcgcg cgccgcgatc tgctgcgcca cccagccggc
     5341 gccgccggac gaaatgccgc caaagatgcc ctcttcgcgc gccaaccgcc gcgccatgtc
     5401 ctcggcgtcg agctgatcga ccagcaccag ttcgtccacc gccttcgggt cgtagatttt
     5461 gggcaggtat tcctcgggcc acttgcggat gcccggaatg cgcgagcctt cggccggctg
     5521 cgcgccgatg atctgcacct tgggattttt ctctttcaga aagcgcgaca caccggtgat
     5581 ggtgcccgtg gtgcccatgg cgctgacgaa gtgcgtgatg cgcccgtcgg tctgttccca
     5641 cagttcgggg cctgtggttt cgtagtggat gcgcgggttg tccgggttgg caaactgatc
     5701 cagcacgcgg cccttgccct gcgccaccat cttgtcggcc aggtcgcgcg cgtattccat
     5761 gccgcccgag cgcggcgtca ggatcagctc ggcgccaaac gccttcatgg tctgcgcgcg
     5821 ttcgaccgac aagtcctccg gcatgatcag caccatgtgg tagcccttga tcgccgccgc
     5881 catcgccagc gcaatgccgg tgttgccgct ggtggcttcg atcaaggtgt cgccgggctt
     5941 gatctcgccg cgctcctcgg cacggcggat catcgacagc gcggggcggt ctttgacgga
     6001 gccggccggg ttgttgccct ccagcttggc cagcaccacg ttgccgcgcg cggcgttccg
     6061 ctcggcatcc aggcgctgca gccgcaccag cggcgtgcgg cccacggcgt cttcgatcgt
     6121 cgggtaattc atgttcatca ctgtgccata atttcaggct tcccagcgcg tcgccggagt
     6181 ggtgaaattg gtagacgcag gggactcaaa aatagggact tctacgtagt tcttgcttga
     6241 ggccactgcc cgccaagccg catgggacaa ggaattgagt gataaatcgc ctcttaatcg
     6301 agatgcccgc atttcgaacc aagagccagc taggagccag gacgatgcaa gctggctccc
     6361 agtgaggagt agatagttga agtagcatcg tgcgtcatgc ccgagtggtg aaattggtag
     6421 acgcagggga ctcagaccaa atttgagccc tcagggggaa acccccgagg tgaagcccgt
     6481 caaactcggc gaaggccctg gacgatcagt ccgagctaat accgagccaa gccctggagc
     6541 cgaaaggctc ctcggaaggt gtagagagca gacggcgggc acctacggcc gaaaggctat
     6601 ggtgaaggcg tgctccagcc cacgaacgcc gtttcttcgg agacggcggc ggcgaaagcc
     6661 gaagtgggaa gaaaatcccc cgccgcaagg cgtgccggtt cgattccggc ctcgggcacc
     6721 aattcggaag ccacccctgt tcacgcaggg gtggcttttt cgttcttggt ctgtgtgcgc
     6781 ttgaatcccc gatccccctc cataaaggct tccagcatgt acgggatcag cgtggccgta
     6841 tccactgcct cgccatatac ctgcgcgtgc agcgcagcgt agcggtcaag gtcagctttc
     6901 aggctaaccg ggcacgcaaa ggtcagcttg acgctttcgg tcctgggcag cggcccgagc
     6961 cgcagcttct tggtcgtgct catcgaatgg ctccccgctg gaagaacatc ggctggtacg
     7021 gtcgcaacac cagatcgcga ttgacgatga tccgcaccgg caggccaggc cgttcggtca
     7081 gcgtcggctg gatgttcatg ttgcgccggg tcatctcctg gcccacctgg ttgatgctgt
     7141 cctgcgcgct gtcgcgcccg gcaatcacga tgcggttcct gttcagcctc caggcgctcc
     7201 agctcgcgct gcttggcctg gatcatcggg gtctggcgcc gctccttctg ccagggcagg
     7261 tcgaaggtca actggaacga caccatgtcg ccccagcgac gatcgcgtcg ggtataggca
     7321 atttcccaag accaatcgcc acgcgcctcc gactgcgcct cgtgggactc cgcccgtgcc
     7381 atgctttgca tggtcgggta cagcgccagc tcggcatggc tggacagctc ggcgcgcaac
     7441 tgcgcaacgg gatggacgag cggaccggtg ccgccctgca gcgattcgtc ggcgcgcggc
     7501 ccgacccagc gccgcagcat ggctcgtgcc ttggaccggt cgcgctgcag gtcgtcgcgc
     7561 cggtccgaca gcgccagcgc ctcctgctgc gcggtcagca ggtcgcccgc ctgagccgat
     7621 ccgcccgcca cccgcgcgag taggctgtct tgcaggcgcc ggttctcggc cagcagttcc
     7681 gcgagcagtt gatcacgctg ttccacggcc tgcgcggcaa tccacgccag gccgagctcc
     7741 tgccgtatct gcaggcgttg caagaccagc gccgcgcgct cgcgttccac gcgcgcctgg
     7801 gcgctggcca ccttggccgc acgcttggct tggttgggaa cctcctgcat cagggccagg
     7861 cgctgcccgg tcatggattc gcgcgtcagg ctccagcggt ccatgccgct gatggggaag
     7921 ctttcgaggc cgatggaaag cttggggtcc ggcagcgtgc cggcggcctt ctgcgcggac
     7981 tcgaccgcat cgatctgcaa tcgctgcgcg gaaacgcgcg ggctttgctg ttcagcgatt
     8041 tccctcgctt cgacaaagct gagcgcgtag gccccgctgg cggcgagcat gctgccggcc
     8101 agggcgatgc tcaaaacgct cagtcgatgc gtccatgtgc ggcacgaggt cgcacgcact
     8161 acgccacggc caggcgcggg gcgacgctcg ccgggagcgc cggaggaaga aataagcatg
     8221 atggtcctcg aaattgatag ccacgaagtc acgtgttctg ccgtgccaat gcacggcggg
     8281 cacgacttgg tgtgtcaatt acgcaggact tggaaagtga gatggaaggg gaaagagccc
     8341 gttcggtggt cgagccacgc cggatcagcc ctggcgtcgg tcgatgattg cgggtcgggc
     8401 accactggaa gcaggtaggc agtccagacc gcatgcgccg ccgcagtaga tagaccgtca
     8461 agtccttgcg gagaaggcgc gggcttgccg tcaccgctgc aatgggcacg gcacaactgc
     8521 ggagactcgt cgtccatcgt cccgggcatg gcatggcagt ccggcatggc ggacatgccg
     8581 gtggcttcgt ccatggccat gcccgccatc tgcatcgatg ccgcgtgcgt atccgcatcc
     8641 agcttcgggc aggcatacat cgccagcgcc atctgcgaga acagcaaggc cacgatgagg
     8701 ccgccgacga tccggcggcc ccattgcgtg acgatgtgtc ggatggaaac catggtggga
     8761 tgttcgctgt gctggatgtg tgacaacttg ccgcggatca gagagccgtc ccgggaagga
     8821 agtgccttca tggcccatct acccgcaggc ggcccagtca ccgcgcatgc gaggtcaccg
     8881 aatccctgga ttcatttcac cggaatccaa cttgtcggcg cgtctcaatc tcggccagtc
     8941 cgagaacgcg agcacgtaca gtgcgatcag gttgaccatg gggacgatca tcagcaggcc
     9001 gagccagccg gaaaagcccg ccttggcaaa ggtcttccaa aacggcagca cgatcaccac
     9061 cgcacccagc agcatcgtca gccagtgggc acccaaccac caccccatat cgttcatgta
     9121 cattccgttc atcaggatcg gtaacctctt tttgattgtt tcgatgcggg ttaacctcgt
     9181 gcgcgccgca gccgcagcgc gttgaagatg accgaggccg agctcaggct catggccagc
     9241 gccgcgacca tgggcgacag caaccagccc gtgatgggat aaagcacgcc ggcggcgatg
     9301 ggcacgccaa taccgttgta gacgaaggcg aacagcaggt tctgccgcat gttgcgcacc
     9361 gtatcgaggg atatctggcg tgccgccgcg atgccgcgca gatcgccctt gaccagggtg
     9421 acctggccgc tgttcatcgc cacgtcggtg cccgttccca tggccacgcc cacgtcggcc
     9481 ttggccaacg ccggcgcatc gttgatgccg tcacctgcca tcgcgaccac atggcccgcg
     9541 cgctgaaatt tttcgaccag tgccagcttg tcggcgggct tgacctcgcc gtgcacctcg
     9601 tcgatggcca ggcgggtgcc cacggccctg gccgtggtga ggccatcgcc cgtagccatg
     9661 acgatgcgca ggccgccggc gtgcagggtg tggatggcct cgggcgtact ggacttgatg
     9721 gggtcggtca cggccagcag gccggccagc cggccatcga cggccaggtg catgacgctg
     9781 gcgccttcgc cgcgcaggcg ctcggcgtcc gccttcaagg cggacacgtc caggccctcc
     9841 tgctccatga gcgcggtgtt gcctaaggcc aggcgccgcc cctcgaccgt gccgcgcacg
     9901 ccgatgccgc tgcccgactc gaagtcggtc ggcttggcca gttgcaagcc cttttcgcgc
     9961 gccgcgctga cgatggcgtc ggccagcggg tgctcgctgc cctggtccag gctggccgcc
    10021 aggcgcagca cctcgtccgc ggtgtagccc ggggcggcga cggccgtgtc gaaggccggc
    10081 ctgccctcgg tcagcgtgcc tgtcttgtcc acgatcag
//
LOCUS       DAOMVO010000057        10107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1636_length_10107_cov_16.535018, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000057 DAOMVO010000000
VERSION     DAOMVO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1636_length_10107_cov_16.535018"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKV30_04530"
     CDS             <1..226
                     /locus_tag="PKV30_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRL29518.1"
                     /translation="GVAQPITDSDRATARAIGAVLAPRGLLLIGLDIIGTKVTEINVT
                     SPTGFREILDQTGCDVPALYVDAVERAVQS"
     gene            352..984
                     /gene="pssA"
                     /locus_tag="PKV30_04535"
     CDS             352..984
                     /gene="pssA"
                     /locus_tag="PKV30_04535"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRL29519.1"
                     /translation="MTEPPVSKNKPFSMIREFHLADWFTLANAVCGVGAMFAIITFIQ
                     VGDVKHVYYACALVLAALVFDIFDGRVARWRQKSSLLGRELDSLADVISFGVAPALIA
                     YGCGMQGLWDRIVLGFFVACGVSRLARFNVTAEAMSGGGDKVKYFEGTPIPTSILLVF
                     LLWAAAALGAIGPNLWGGAWKIGGFTLHPLVLLFALSGSLMISRIRIPKL"
     gene            1103..2743
                     /locus_tag="PKV30_04540"
     CDS             1103..2743
                     /locus_tag="PKV30_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL29520.1"
                     /translation="MPDSDHAKAAAADDDLGPLAPFRHSVFTMLWSTWLVANLCMWMN
                     DVAAAWLMTTLTSKPIWVALVQTAATLPVFLLGLPSGALADILNRKHFLFFTQVWVAV
                     VGALLALAVFMKVVTPPLLLMLLFMNGVGLALRWPVFSAIVPDLVPRAKLPAALALNG
                     VSMNASRILGPLIAGSIIASLGSAWVFLLNAVLSVGAAVVITRWQREHKPDPLGRESL
                     RGAMRVGLQYVAQSYHLKGVLIRIAIFFFHSTALMALLPLVARQIEGGDAGTFTVLLA
                     AMGAGAIASTFALPRLRQAWSRDKLVLVSAVTQALTMAVMAVNTSLWLGVPAMAIGGA
                     AWLTAANSLSVSAQMSLPDWVRARGMSMYQMAIMGAAASGAAVWGQVATWTSVPWALA
                     LGAVSGSAAMALSMWWWPDRGVIDDPTPAGPMARPEVTTPPGSGHVVVTVEYLIDPAN
                     APAFRALMEESRRSRLRQGAVAWELLSDINEPGRFVEVIEDDSWIDHLRRFERVSASD
                     VALRERKMAYHLGEEPPVVRRAVRESTVRGGRKVFEPN"
     gene            complement(2799..3566)
                     /locus_tag="PKV30_04545"
     CDS             complement(2799..3566)
                     /locus_tag="PKV30_04545"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRL29521.1"
                     /translation="MCQLLGMNCNTPTDVTFSFAGFAQRGGRTDHHADGWGIAFFEGS
                     GARLFIDPSAAADSPVAELIRNYPIKSRNVISHIRKATVGAVMLENCHPFVRELWGRY
                     WVFAHNGDLKDYAPRLHGHFRPVGSTDSEQAFCWLMQELAKSHARVPPVDELTRTLRE
                     LMPQVARHGTFNMLLSNGEALWAHASTKLCYIVRQHPFASARLADEELAVNFAEHTTP
                     DDRVAVVATTPLTRDEQWTPFEPGELKVFVDGAPLAA"
     gene            3627..3986
                     /locus_tag="PKV30_04550"
     CDS             3627..3986
                     /locus_tag="PKV30_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29522.1"
                     /translation="MNTIHRALHASLVAAALTLAGVAHAQTAPAELGLSLAATSGCLN
                     CHGTAPRGDAPSFLHMQERAAGREGDRDALARHWMQEMRATGSGWRAIVAHRQVTDST
                     AGALFRWLGTPPTKPVN"
     gene            complement(3979..4200)
                     /locus_tag="PKV30_04555"
     CDS             complement(3979..4200)
                     /locus_tag="PKV30_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HRL29523.1"
                     /translation="MTAAPATDPSRCPLCGAANGCAMEAERASGQPQPPCWCTQVAFD
                     ADLLARVPADARRLACICAACAAAAERVS"
     gene            4272..4643
                     /locus_tag="PKV30_04560"
     CDS             4272..4643
                     /locus_tag="PKV30_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRL29524.1"
                     /translation="MTQALTAPQRDELKQLLLTRRKELQRQMTQNLDNLAPSDSGDSV
                     SQDDDARLKNQTREVDGALTAMDADELARIDRALEAMDDGEYGVCVVCGKDIPFERLK
                     VEPMTQHCIVDKAKLEQQARA"
     gene            complement(4736..5035)
                     /locus_tag="PKV30_04565"
     CDS             complement(4736..5035)
                     /locus_tag="PKV30_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29525.1"
                     /translation="MTLSYLIFDASDDGGGTGSWEAMASVRAPDVAAVLAEAQQVIDA
                     AQAQSPGPRAPLDEGGAWDALLQLQPEGDWTVVTLTLTGPWEWGEALLAALAPDA"
     gene            complement(5106..7328)
                     /locus_tag="PKV30_04570"
     CDS             complement(5106..7328)
                     /locus_tag="PKV30_04570"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HRL29526.1"
                     /translation="MTERTPVHDLQVATDLYNFVNDEVLPDVGVAPGDFWHGFSSLVT
                     ELAPKNAALLAERDRLQAELDTWHRANPGPIKDMRAYRAFLEGIGYLVPQPDKVQITT
                     TNVDDELAVQAGPQLVVPILNARYALNAANARWGSLYDALYGTDVIPETKGLEKGAKY
                     NAKRGAKVIEWARYVLDRTAPLKKGSHIDSTGYVVNKDGELVVKLKGGKSSKLVDNAQ
                     FVGYQGPAKAPTSILLVHNGLHIDIRIDPKSPIGKTDPAGVADLVVEAAVSTILDLED
                     SVACVDAQDKVLGYRNWLGILRGTLTESFDKGGKIVKRGLNPDRVYTAPDGEGEVTLH
                     GRSLMFVRNVGHLMTNPAILWKDAAGEWNEIPEGFLDAAITTAIAMHDLQGKGADGLR
                     NSRTGSVYIVKPKMHGPAEVAFASELFGRVEKLLHLPYNTVKLGIMDEERRTSVNLKA
                     CIDAAKSRVAFINTGFLDRTGDEMHSCMMAGPVYRKGDMKTSAWIAAYERNNVLTGLA
                     CGLRGKAQIGKGMWAMPDLMAEMMKQKIGHPKAGANTAWVPSPTAATLHAMHYHQVLV
                     SDVQQQMETELAKADPAALRDELLTGLLSIPVVAQAKWTPQEKQQELDNNAQGILGYV
                     VRWVDQGVGCSKVPDIHGVGLMEDRATLRISSQHMANWLLHGVVSKGQVEATLQRMAA
                     LVDQQNSGDKLYRNMHGNFKKSAAYQAACDLVFKGIEQPSGYTEPLLHAWRQKVKAGA
                     "
     gene            7503..9431
                     /locus_tag="PKV30_04575"
     CDS             7503..9431
                     /locus_tag="PKV30_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HRL29527.1"
                     /translation="MPVFDPQQIQHSPYKNKVVSAAEAVQLIHDGDTVATGGFVGIGF
                     AEGVAVALEERFISSQHPRDLTLVYAAGQGDGKTRGLNHFGHAGLLKRVIGGHWGLVP
                     AVQKLAVDNLIEAWNLPQGVICHLFRDIAAGKPGTLTRVGLDTFVDPRHGGGKLNART
                     TDDLVQLLKIGDEECLLYKAFPIQVGIIRATTADPDGNLTMEREALTLEALAIAMAAR
                     NSGGVVIAQVERIAERGTLHPRQVKVPGVLVDAVVLAQEPAHHQQTFAEPYNPAFAAE
                     IRVPIDRLPPMGLSERKVIARRAALELRPHQVVNLGIGMPEGVANVAAEEKIIDLLTL
                     TAEPGVIGGLPAGGLNFGAAVNPQAIIDQPYQFDFYDGGGLDIAVLGLAQADSHGNLN
                     VSKFGPRLAGAGGFINISQNARTVVFVGTFTAGQLEVRLDGGQVAIAREGGARKFVRE
                     VEHRTFSGAEAVRRGQRVLYVTERAVFRLVPQGDGAGGELELCEIAPGIDLQRDVLAH
                     MGFQPRIAADLKTMDARLFRPEPLGLRAQLLERPLAERMQLDRAARVLHIDFSGLQVN
                     SSATIAAIDAAVRALLQPFGERVDAVVNYDHFGIVPELVDPYADMVHRLAADWYQRVT
                     RYGTTGFVKSRLKPSPGD"
     gene            complement(9612..>10107)
                     /locus_tag="PKV30_04580"
     CDS             complement(9612..>10107)
                     /locus_tag="PKV30_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29528.1"
                     /translation="ADIPTLTVRAEAASAQAARALPSLGTPPDATPDAPRPRHDALAD
                     QLQLMAQQDEARERAHGPPRLVGMVLIAWGVLLLFGALVMLGRGGGAYYLFCAVGSAA
                     VGWLLMQSSRWAMALHGLLLLLALAWAWRANGGSLAMAVVQAAPLWIPALWMAVRPVR
                     EPLE"
BASE COUNT         1534 a         3378 c         3524 g         1671 t
ORIGIN      
        1 gggtgtggcc cagcccatca ccgacagtga ccgcgccacg gcgcgcgcca tcggcgcggt
       61 gctggcgccg cgcggactgc tgttgattgg cctggacatc atcggcacca aggtcacgga
      121 gatcaacgtc accagcccca cgggctttcg cgagatcctg gaccaaaccg ggtgtgacgt
      181 gccggcgctg tatgtggatg cggtggagcg ggcggtgcag tcgtagacgg catctgccga
      241 gccttgcgac cgtcatgcgg tcctcatggc ggaaagcaaa ggcccgcagc gggcctgatt
      301 tgcgccattg actgacacat gaccacgccg gcatcggtgc aaactccacc gatgaccgag
      361 ccgcccgttt cgaagaacaa acccttctcc atgatccgcg agttccacct cgcggactgg
      421 ttcacgttgg ccaacgccgt ttgcggcgtg ggcgcgatgt ttgccatcat caccttcatc
      481 caggtgggcg atgtgaaaca cgtgtactac gcctgcgcgc tggtgctggc ggcgctggtg
      541 ttcgacatct tcgatggccg tgtggcgcgt tggcggcaga agtcgtcgct gctcgggcgc
      601 gagctggatt cgctggccga cgtgatttcg ttcggcgtcg cgccggcgct cattgcctac
      661 ggttgcggca tgcaggggct gtgggaccgc atcgtgctgg gcttcttcgt cgcttgcggc
      721 gtgtcgcggc tggcgcgctt caacgtcacg gccgaggcca tgtcgggcgg tggcgacaag
      781 gtgaagtact tcgagggcac gccgatcccg acctcgatcc ttctggtgtt tctgctgtgg
      841 gccgccgccg cgctcggcgc catcggcccc aacctgtggg gcggcgcgtg gaagatcggc
      901 ggcttcacgc tgcacccgct ggtgctgctg tttgcgctgt cgggctcgct gatgatcagc
      961 cgcatccgca ttcccaagct gtgatgcttt tgagcgaaat aggccgctgg cgcctgccaa
     1021 tcaagcgcga acagctataa aaacaatagt ttcaggcggc gccacacacg ccgcgccggg
     1081 gccgctctat catcggtggc ccatgccgga ttcagaccac gccaaagccg ccgccgcgga
     1141 cgacgacctc ggcccactgg cgccgtttcg ccacagcgtg ttcaccatgc tgtggagcac
     1201 ctggctggtt gccaacctgt gcatgtggat gaacgacgtg gcggcggcgt ggctgatgac
     1261 cacgctcacg tccaagccga tctgggtcgc gctggtgcag accgcggcca cgctgccggt
     1321 gtttctgctc gggctgccct cgggcgcgct ggccgacatc ctgaaccgca agcacttttt
     1381 gttcttcacg caggtctggg tggcggtggt gggcgcgctg ctggcgttgg cggtgttcat
     1441 gaaggtggtg acgccgccgc tgttgttgat gctgctgttc atgaacggcg tcggcctggc
     1501 gctgcgctgg ccggtgtttt cggccatcgt gcccgacctg gtgccgcgcg ccaagctgcc
     1561 ggcggcgctg gcgctcaatg gcgtgtcgat gaacgcctcg cgcatcctcg ggccgctgat
     1621 cgccggcagc atcatcgcca gcctgggcag cgcctgggtg tttctgctga acgcggtgct
     1681 gtcggtcggc gcggcggtgg tcatcacgcg ctggcagcgc gaacacaagc ccgacccgct
     1741 ggggcgcgag agcctgcgcg gcgccatgcg cgtgggcctg cagtacgtgg cgcagtcgta
     1801 ccacctgaag ggcgtgctga tccgcatcgc catcttcttc tttcactcca ccgcgctgat
     1861 ggcgctgctg ccgctggtgg cgcgccagat cgagggcggc gacgccggca ccttcaccgt
     1921 gctgctggcg gccatgggcg cgggcgccat cgcctcgacc tttgcgctgc cgcgcctgcg
     1981 ccaggcctgg tcgcgcgaca agctggtgct ggtgagcgcc gtcacgcagg cgctgaccat
     2041 ggcggtgatg gccgtcaaca ccagcctgtg gcttggcgtg cccgccatgg cgattggcgg
     2101 cgcggcgtgg ctcacggcgg ccaactcgct cagcgtgtcg gcgcagatga gcctgcccga
     2161 ctgggtgcgc gcgcgcggca tgtcgatgta ccagatggcc atcatgggcg cggccgcgtc
     2221 cggcgccgcc gtgtggggcc aggtggccac ctggaccagc gtgccgtggg cgctggcact
     2281 gggcgcggtc agcggcagcg cggcgatggc gctcagcatg tggtggtggc cggatcgcgg
     2341 cgtgatcgac gacccgacgc ccgccggccc gatggcgcgg cccgaagtga ccacgccgcc
     2401 cggcagcggc cacgtggtgg tgacggtgga atacctgatc gacccggcca acgcgccggc
     2461 gtttcgcgcg ctgatggaag aaagccgccg ctcgcgcctg cgccagggcg cggtggcgtg
     2521 ggaactgctg tcggacatca acgagccggg gcgctttgtc gaggtgatcg aagacgattc
     2581 gtggatcgac cacttgcggc gcttcgagcg cgtgagcgcg tccgacgtgg cgctgcgcga
     2641 gcgcaagatg gcgtaccacc tgggcgagga gccgcccgtg gtgcgccgcg cggtgcgcga
     2701 aagcacggtg cgcggcgggc gcaaggtttt tgagccaaat tgagcactgg cgcttgttaa
     2761 gcaagcgcga gcagctatca aatcaatagt tacgacgatc aggcggcgag cggcgcgccg
     2821 tcgacgaaca ccttcaactc gcccggctcg aagggcgtcc actgctcgtc gcgcgtgagc
     2881 ggcgtggtgg ccaccacggc cacgcggtcg tcgggcgtgg tgtgctcggc aaagttgacg
     2941 gccagctcct cgtcggccag ccgcgcgctg gcaaacgggt gctggcgcac gatgtagcag
     3001 agctttgtcg acgcgtgcgc ccacagcgcc tcgccgttgg acagcagcat gttgaaggtg
     3061 ccgtgccggg ccacctgcgg catcagctcg cgcagcgtgc gcgtcagctc atcgaccggc
     3121 ggcacgcgcg cgtgcgactt ggccagctcc tgcatcagcc agcaaaaggc ctgctcgctg
     3181 tcggtgctgc cgacggggcg aaagtgcccg tgcaggcgcg gcgcgtagtc cttcaggtcg
     3241 ccgttgtgcg caaacaccca gtagcggccc cacagctcgc gcacgaaggg gtggcagttc
     3301 tccagcatca ccgcgcccac ggtggccttg cggatgtgcg agatgacgtt gcggctcttg
     3361 atggggtagt tgcggatcag ctcggccacc ggcgaatcgg ccgccgcgct ggggtcgatg
     3421 aacaggcgcg cgccgctgcc ttcgaaaaac gcgatgcccc agccgtcggc gtggtggtcg
     3481 gtgcggccgc cgcgctgcgc gaaaccggcg aaggaaaagg tcacgtcggt cggcgtgttg
     3541 cagttcatcc cgagcagctg gcacatggcg gcgattatgc ggcgcggcct gttccacgcg
     3601 cgccccgcag cgctatcatg aacggcatga acacgattca ccgcgcgctg cacgccagcc
     3661 tggtcgctgc agcgctgacg ctggccgggg tggcgcacgc gcagaccgcg ccggccgagc
     3721 tgggcctcag cctggccgcc acgtcgggct gcctgaattg ccacggcacg gcgccgcgcg
     3781 gcgacgcgcc ctcgttcctg cacatgcagg agcgcgccgc cggccgcgag ggcgaccgcg
     3841 atgcgctggc ccgccactgg atgcaggaga tgcgcgccac cggcagcggc tggcgcgcca
     3901 tcgtcgcgca ccgtcaggtg accgacagca cggcgggcgc cttgttccgg tggctgggca
     3961 cgccgccgac caaaccggtc aactgacgcg ctcggccgcc gccgcgcagg cggcgcaaat
     4021 gcaggccagc cgccgcgcat cggccggcac gcgcgccagc aaatccgcgt cgaaggcgac
     4081 ctgcgtgcac cagcacggcg gttgcggctg gccgctggcg cgctcggctt ccatggcgca
     4141 gccattggcc gcgccgcaca gcgggcagcg gctcgggtcg gtcgcggggg cggcggtcat
     4201 gtgggtggta ttctgaggga caaggcgcct gcgcgaacgg ttcgcggcgc ccaagcattc
     4261 aggagcaacc catgacccag gccctcaccg cgccgcagcg cgatgaactc aagcaactgc
     4321 tgctgacgcg ccgcaaggaa ttgcagcgcc agatgacgca gaacctcgac aacctggcgc
     4381 catccgacag cggcgacagc gtgtcgcagg acgacgatgc ccgcctgaag aaccagacgc
     4441 gcgaagtcga cggcgcgctg accgcgatgg acgccgacga attggcgcgc atcgaccgcg
     4501 cgctggaggc gatggacgac ggcgaatacg gcgtgtgcgt ggtgtgcggc aaggacattc
     4561 cgttcgagcg cctgaaggtc gagccgatga cgcagcactg catcgtcgac aaagccaagc
     4621 tggagcagca ggcgcgcgcc tgagcgggtt ctcaataaaa agcggcccca gcgcttgagg
     4681 gacaagcgcg agcagctatc aaaaaaagag tgatgcgtct tggctgaaat cgccatcacg
     4741 cgtccggcgc cagcgccgcc agcagcgcct cgccccattc ccacggcccg gtcagcgtga
     4801 gcgtgacgac ggtccagtcg ccctcgggct gaagctgcag cagcgcgtcc cacgcgccgc
     4861 cttcgtccag cggcgcgcgc gggccgggcg actgggcttg ggcggcgtcg atcacctgct
     4921 gcgcctcggc cagcacggcg gcgacatcgg gcgcacgcac gctggccatg gcttcccagc
     4981 tgccggtgcc gccgccgtcg tcgctggcgt cgaaaatcag gtaactcaaa gtcaagcggc
     5041 gattttgacc gcgccatgaa aaaagcgacg ccagggcgtc gcttgatcgg ccaagcgcgt
     5101 gcgcgttatg cgccggcctt caccttctgc cgccaagcat gcagcagtgg ctcggtgtag
     5161 ccgctcggct gctcgatgcc cttgaacacc aggtcgcacg cggcctggta cgcggccgat
     5221 ttcttgaagt tgccgtgcat gttgcggtac agcttgtcgc cgctgttctg ctgatcgacc
     5281 agcgccgcca tgcgctgcag cgtggcttcg acctggccct tgctgacgac gccgtgcagc
     5341 agccagttgg ccatgtgctg gctggagatg cgcagcgtgg cgcggtcttc catcaggccg
     5401 acgccgtgga tgtcgggcac cttggagcag cccacgccct ggtcgaccca gcgcaccacg
     5461 tagccgagga tgccctgcgc gttgttgtcc agctcctgct gcttttcctg cggtgtccac
     5521 ttggcttggg ccaccacggg gatgctgagc aggcccgtga gcagctcgtc gcgcagcgcc
     5581 gccgggtcgg ccttggccag ttcggtttcc atctgctgct gcacgtcgct caccagcacc
     5641 tggtggtagt gcatggcgtg cagcgtggcg gcggtcgggc tgggcaccca ggcggtgttg
     5701 gcgccggcct tggggtggcc gatcttctgc ttcatcatct cggccatcaa atcgggcatg
     5761 gcccacatgc ccttgccgat ctgcgccttg ccacgcaggc cgcaggccag gccggtgagt
     5821 acgttgttgc gctcgtaggc cgcaatccag gcgctggtct tcatgtcgcc cttgcggtac
     5881 accgggccgg ccatcatgca gctgtgcatt tcgtcgccgg tgcggtcgag aaagcccgtg
     5941 ttgatgaagg ccacgcggct cttggcggcg tcgatgcagg ccttcaggtt gaccgacgtg
     6001 cggcgctctt cgtccatgat gccgagcttg acggtgttgt agggcaggtg cagcagcttc
     6061 tcgacgcggc cgaacagctc gctggcaaag gccacctcgg ccgggccgtg catcttgggc
     6121 ttgacgatgt agacgctgcc ggtgcgcgaa ttgcgcaggc catcggcgcc cttgccctgc
     6181 aggtcgtgca tggcaatcgc cgtggtgatg gcggcgtcca gaaagccttc ggggatttca
     6241 ttccactcgc cggcggcgtc cttccacaag atggccgggt tggtcatcaa atggccgacg
     6301 ttgcgcacga acatgagcga gcggccgtgc agcgtcacct cgccttcgcc atcgggcgcc
     6361 gtgtagacgc ggtcggggtt gagcccgcgc ttgacgatct tgccgccctt gtcgaaactt
     6421 tcagtcagcg tgccgcgcag aatgcccagc cagttgcggt agccgagtac cttgtcctgc
     6481 gcgtcgacgc acgccaccga gtcttccagg tcgaggatgg tcgagacagc tgcttccacc
     6541 accagatcgg ccacgcccgc cggatcggtc ttgccgatgg gcgacttggg gtcgatgcga
     6601 atgtcgatgt gcaggccgtt gtgcaccagc aggatcgagg tcggcgcctt ggccggcccc
     6661 tggtagccca cgaactgcgc gttgtcgacc agcttgctcg acttgccgcc cttgagcttc
     6721 acaacaagct cgccgtcctt gttgacgacg tagccggtcg agtcgatgtg cgagcctttc
     6781 ttgagcggcg cggtgcggtc gagcacgtag cgcgcccact cgatcacctt ggcgccgcgc
     6841 ttggcgttgt acttggcgcc tttttcgagg cccttggttt cggggatcac gtcggtgccg
     6901 tacagcgcgt cgtacaggct gccccagcgc gcgttggcgg cgttcagcgc gtagcgggcg
     6961 ttcaggatcg gcaccaccag ctgcgggccg gcctgcacgg ccagctcgtc gtcgacgttg
     7021 gtggtggtga tctgcacctt gtccggctgc ggcaccaggt agccaatgcc ttcgaggaag
     7081 gcgcggtagg cgcgcatgtc cttgatgggg cccgggttgg cgcggtgcca ggtgtccagc
     7141 tcggcttgca ggcggtcgcg ctcggccagc agggcggcgt tcttgggtgc cagctcggtc
     7201 accagggacg agaaaccgtg ccagaaatcg cccggcgcca cgcccacatc gggcagcacc
     7261 tcatcgttga cgaagttata cagatcggtg gcgacctgca ggtcgtggac gggggtgcgt
     7321 tcggtcatgg cgggcttttt gaagaagatg aacaaatcgc gcgggtggcg caggctgaag
     7381 tccactttat tcgatttgtt ttgagcaata aagaatcatg aggctgaaaa acctttgact
     7441 tttttgcaag tatcggccga caccgcgcgg ccttatgctg ccggcacacc gaggagaccc
     7501 gcatgcccgt cttcgatccg cagcagatcc agcacagccc gtacaagaac aaggtcgtca
     7561 gcgccgccga ggcggtgcag ctgattcacg atggcgacac cgtggccacc ggcggctttg
     7621 tcggcatcgg ctttgccgaa ggcgtggcgg tggcgctgga agagcgcttc atctcctcgc
     7681 agcacccgcg cgacctgacg ctggtctacg ccgccggcca gggcgacggc aagacgcgcg
     7741 ggctgaacca ctttggccac gccggcttgc tgaaacgcgt gatcggcggc cactggggtc
     7801 tggtgcccgc ggtgcaaaag ctcgcggtcg acaacctgat cgaggcctgg aacctgccgc
     7861 aaggcgtgat ctgccatttg ttccgcgaca tcgccgccgg caagcccggc acgctcacgc
     7921 gcgtggggct ggacaccttt gtcgacccgc gccacggcgg cggcaagctg aacgcgcgca
     7981 ccaccgacga tctggtgcaa ctgctgaaga tcggcgacga ggaatgcctg ctctacaagg
     8041 cctttccgat ccaggtcggc atcatccgcg ccaccaccgc cgaccccgac ggcaacctga
     8101 ccatggaacg cgaggcgctg acgctggagg cgctggcgat tgccatggcg gcgcgcaact
     8161 cgggcggcgt ggtgatcgcg caggtcgagc gcattgccga gcgcggcacg ctgcacccgc
     8221 gccaggtcaa ggtgccgggc gtgctggtcg atgcggtggt gctggcgcaa gagccggcgc
     8281 accaccagca gacctttgcc gagccctaca acccggcctt tgcggccgaa atccgcgtgc
     8341 cgatcgaccg cctgccgccc atgggcctgt ccgagcgcaa ggtcatcgcg cggcgcgccg
     8401 cgctggagtt gcgaccgcac caggtggtca acttggggat cggcatgccc gaaggcgtgg
     8461 ccaacgtggc ggcggaagaa aaaatcatcg acctgctgac gctgacggcc gagccgggcg
     8521 tcatcggcgg cttgccggcg ggtggactca actttggcgc ggcggtgaac ccgcaggcca
     8581 tcatcgacca gccctaccag ttcgacttct acgacggcgg cgggctcgac atcgccgtgc
     8641 tgggtctggc gcaggccgac agccacggca acctgaacgt gagcaaattc ggcccgcgcc
     8701 tcgccggcgc gggcggcttc atcaacatca gccagaacgc gcgcaccgtg gtgtttgtcg
     8761 gcaccttcac cgccggtcag ctggaggtgc ggctggatgg cggacaagtg gccatcgcgc
     8821 gcgaaggcgg cgcgcgcaag ttcgtgcgcg aggtcgagca ccgcaccttc agcggtgccg
     8881 aggccgtgcg gcgcggccag cgcgtgctgt acgtgaccga gcgcgccgtg ttccgcctgg
     8941 tgccgcaggg tgatggcgcc ggcggcgagc tggagctgtg cgagatcgcg cccggcatcg
     9001 atctgcagcg cgacgtgctg gcccacatgg ggttccagcc gcgcatcgcg gccgatctga
     9061 agaccatgga cgcgcgcctg ttccgccccg aaccgctcgg cctgcgcgcg cagttgctgg
     9121 agcgcccgct ggccgagcgc atgcagctgg accgcgcggc gcgcgtgctg cacatcgatt
     9181 tctccggcct gcaggtcaac tcgtcggcca ccatcgccgc catcgacgcg gcggtgcgcg
     9241 cgctgctgca accgtttggc gagcgggtcg acgcggtggt gaattacgac cacttcggca
     9301 tcgtgcccga gctggtcgat ccctacgccg acatggtgca ccggctggcc gccgactggt
     9361 accagcgcgt cacgcgctac ggcaccacgg gcttcgtcaa gtcgcggctg aagccgtcgc
     9421 caggcgattg attcgatgac gaaatgactt cgctgcgctc catgacggaa tgaccaggag
     9481 cccgtcatgg cgcgccgcgc cgtcatcgat ggcgcagccg aggtcatgcg ccgtcaagcg
     9541 cggtcatcat caaaaatgat agctgctcgc gcttgctgga cgtgcgccag cggccgattg
     9601 ggcttgaaag atcactccag cggctcgcgc accggccgca ccgccatcca cagcgccgga
     9661 atccacagcg gcgccgcctg caccaccgcc atcgccaggc tgccgccgtt ggcgcgccag
     9721 gcccaggcca gcgccagcag caacagcagg ccatgcagcg ccatggccca gcggctgctt
     9781 tgcatcagca gccagccgac ggccgccgag ccgacggcgc agaacaggta gtaggcgccg
     9841 ccgccgcgcc ccagcatgac cagcgcgcca aacagcagca acacgcccca ggcgatcagc
     9901 accatgccga ccaggcgcgg cgggccgtgc gcgcgctcgc gtgcctcgtc ctgctgcgcc
     9961 atcagctgca gctggtcggc cagcgcgtcg tggcgcgggc ggggcgcgtc cggcgtggcg
    10021 tctggcgggg tgcccagcga cggcagcgcg cgcgcggcct gcgccgacgc tgcctcggcg
    10081 cgcaccgtca gcgtggggat gtcggcc
//
LOCUS       DAOMVO010000058        10078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1644_length_10078_cov_11.701487, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000058 DAOMVO010000000
VERSION     DAOMVO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_10078_cov_11.701487"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /locus_tag="PKV30_04585"
     CDS             complement(<1..2552)
                     /locus_tag="PKV30_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRL29529.1"
                     /translation="MYGDSQSTSTAPAAEAAAPSVISSPAGRRWLPANRWARRALYGV
                     AGLLGLWALGWLAVPPLLKWQGEKIASEQLGRAVRIGAVDFKPWTLELTLNDVAVGGA
                     AGAPDQLSVKRIYANAALQSLFRLAPVVDAIQVEAPALRLAHLGYGHYDIDDILAKLA
                     ARPQSVEATEQARFALYNIDLSGGRIDFDDRAVKRQHEVRDLRLTLPFVSNLPSERQI
                     KVQPQLAFVANGSAFDSRAEALPFADSRQTDAAFRLTQLDLKPYLGYWPASLPIKLQS
                     AVVDADLRLDFEQTPKPSLRLSGAQAVSGLKSVDGQGADALAFDALKVQLAEVRLLQR
                     KVQVSSVELSGPQLALRRLKDGQFNLLVVPGGADATKSEAAGASPTSAGGISDAASAP
                     RPAASAAAPAPAWQVAIDKLALHGGTLDFVDDTTAVDGAPAAVVRLNEVELTAARMGL
                     PISQPLSFNGSAALVGTEELTPAIPDAAAPRAAAPRRARKGAATEAAAPQAPASATRS
                     AVPSIEFQGTAAAREVEVNARVAGLPLALAAPYLAPHLVPQLTGDLDAHVGLRWAPPQ
                     AQGLPPELKVAAESLTLRQLMLADAPTPAASAAPPPRPARGVKHSGPLASIGQIDVAE
                     LLVDLRSRAVTVGKLAVQTPRVVVERGADNALMYQAWLRQPPGAAAPDNAAKDKAKPR
                     PRGGPAADFPWKVLVSELAVAGGNVGWRDAATPTPVEAELSQLRIDAKQVDLNSATPI
                     PVNLIARISGGRGGTEPGRVSWRGSVGLTPLTAQGAIDAERLPVHAFEPYFGDALNID
                     ILRADTSFKGNVAFAHLTAGPRLRVSGDASVQELRTHSRPGSAAASDGPQAAA"
     gene            3452..4282
                     /locus_tag="PKV30_04590"
     CDS             3452..4282
                     /locus_tag="PKV30_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL29530.1"
                     /translation="MTASGYLAATARGIKTFAGDVGEGFFEITHNTLALLGIVVVVVV
                     ALLGTRPDLRAQGEQRLTAWLTARQVAAMGITPELGAIERATAANPRDLPREQANVAL
                     WISKKYRVAPEPVAALVAEAYDVGRANKLDPTLILAVMAIESSFNPFAQSPVGAQGLM
                     QVMTQIHSDKYEHFGGQYAAFDPKSNLRVGVKVLSEYVTRTGSVEGGLKWYVGAANLP
                     TDGGYADKVMAEHSRLSHVAKGKTIPLTDNTPALRAATTPAPSAAPAGNAPVKVAALS
                     "
     regulatory      4328..4447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            4482..5732
                     /gene="glyA"
                     /locus_tag="PKV30_04595"
     CDS             4482..5732
                     /gene="glyA"
                     /locus_tag="PKV30_04595"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRL29531.1"
                     /translation="MFNRSTHTIAATDADVWAAIQAEHQRQEDHIELIASENYASPAV
                     LAAAGTQLTNKYAEGYPGRRYYGGCEYVDVVEQLAIDRVKQLFGSQAANVQPHCGASA
                     NEAVFLAFLKPGDTIMGMSLAEGGHLTHGMPLNISGKWFNVVSYGLNAQEALDYDAME
                     KKAHETKPKLIIAGASAYSLAIDFERFAKVAKDVGAIFMVDMAHYAGLIAGGQYPNPV
                     PHADVVTSTTHKSLRGPRSGFILMKSQHEKAINSAVFPGLQGGPLMHIIAAKAIAFKE
                     ALQPEFKQYAQQVKKNAQVVAETLTQRGLRIVSGGTESHVMLVDLRAKGITGKEAEAV
                     LGSAHMTINKNAIPNDPEKPMVTSGVRIGTPAMTTRGFGETEARATANLVADVLDNPG
                     VEANLNTVRAKVAELTKRFPVYGG"
     gene            5806..6255
                     /gene="nrdR"
                     /locus_tag="PKV30_04600"
     CDS             5806..6255
                     /gene="nrdR"
                     /locus_tag="PKV30_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRL29532.1"
                     /translation="MRCPFCAHTETQVVDTRVSDEGDFIRRRRQCGHCAKRFTTYERP
                     DVSFPSIVKKDGRRVPYSKPKLQGSLDLALRKRPVSTEQIDAAIERIEEKLLSAGARE
                     VPSSRLGELVMRELKKLDKVAYVRFASVYRSFEDIDEFRALVDEVRR"
     gene            6310..7035
                     /locus_tag="PKV30_04605"
     CDS             6310..7035
                     /locus_tag="PKV30_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29533.1"
                     /translation="MPTVPASHFVPPVRGWPLGAAVLASVLLHLAAWFWIQPTRVAPR
                     AGGAEKPMRISLHTLPPRPAAPVPAPPLPRATPRAQTQAAPPAPRADVRQSPPEIAPP
                     LAAETPPEPPAPAGAATPSNMLYVPAALADQAPVPLGDWVLAEEPWPDGVGAVQITLW
                     IDAEGRIERTLLLGAAADDPRVQALFARIADTPMQPARVGTGDVHSVMTVEIWDETTS
                     GSTVWGTAPADAASGSARTLSNR"
     gene            complement(7294..7740)
                     /gene="trxC"
                     /locus_tag="PKV30_04610"
     CDS             complement(7294..7740)
                     /gene="trxC"
                     /locus_tag="PKV30_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HRL29534.1"
                     /translation="MSEPLHIVCPHCSTTNRVKPDQLASHPDCGQCHQPLFTGHPVAL
                     DDEAAFDKHVLRSQIPVLVDFWAPWCGPCRMMAPAYEKAAADLEPQVRVAKVNTEAAQ
                     ALGGRYNIRSIPTLALFVGGKEVARQPGAMTNPAQIVQWVMAHLPR"
     gene            complement(7766..8095)
                     /locus_tag="PKV30_04615"
     CDS             complement(7766..8095)
                     /locus_tag="PKV30_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29535.1"
                     /translation="MKTLQSPSRTAAVLAQLLERLDASRTSVDAHQYRTVVARLSDML
                     ADPDVDWQPLLDESAVAAAIYENLHYAEAGLCRSPLEAATRAEVAARALIDAVRSKPA
                     PDAAPAA"
     gene            complement(8221..8457)
                     /locus_tag="PKV30_04620"
     CDS             complement(8221..8457)
                     /locus_tag="PKV30_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRL29536.1"
                     /translation="MNDAAREPHAAADLQLLVDWAMPFGKYKGTLLADLPSHYLGWFA
                     QKGFAKGQLGRLLALMYEVDQAGARRLLDPLRRP"
     gene            complement(8470..9666)
                     /locus_tag="PKV30_04625"
     CDS             complement(8470..9666)
                     /locus_tag="PKV30_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL29537.1"
                     /translation="MTFPFAQRLDNVETSAIRELFKLLGKPGIISFAGGFPDSAMFDV
                     HGITEAVNKALIDEPGAALQYGATEGYQPLREQLAAFMQAKGAQVAADQLIVTTGSQQ
                     ALDLLGKCLVDPDSKAIVEGPTFLATIQCFRLYGAELISAPIDGEGVQVDELERLITE
                     HKPKFVYLIPTFGNPSGAMLSLERRKRVLELAVKYQTLIIEDDPYGDLYFDAPPPPSL
                     LALSAQVPGSRDWLVHCGSLSKVLSPGLRVGWMVAPPELLAKATMCKQFSDAHTSTFA
                     QATAAQYLAGGHMPGTLARVREVYGARGKTMGDALRNDLGAEIDFVQPQGGLFVWARL
                     TGAGGKIKDGNEFAKRAIAEGVAFVPGAPFYCANPDLSTLRLSFATADEGKIREGIGR
                     LAKALG"
     gene            9729..>10078
                     /locus_tag="PKV30_04630"
     CDS             9729..>10078
                     /locus_tag="PKV30_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRL29538.1"
                     /translation="MTPAQIETFFATLQAINPLPASELEYTSVFELLAAVLLSAQATD
                     VGVNKATRRLFPVANTPQQIIDLGLERLEGYIKTIGLYRSKAKHLMETCRILVERHGG
                     QVPRTRAELEALPGV"
BASE COUNT         1665 a         3508 c         3361 g         1544 t
ORIGIN      
        1 gccgcggcct gcggcccgtc ggacgcggcg gcgctgccgg ggcggctgtg ggtgcgcagc
       61 tcttgcacgc tggcgtcgcc gctcacgcgc aggcgcgggc ccgccgtcag gtgcgcgaag
      121 gcgacgttgc ccttgaagct ggtgtcggca cgcaggatgt cgatgttgag cgcatcgcca
      181 aagtagggct cgaaggcgtg caccggcagc cgctcggcat cgatcgcgcc ttgcgccgtc
      241 agcggcgtca aacccacgct gccgcgccag ctcacgcggc cgggttcggt gccgccccgg
      301 ccgcccgaaa tgcgggcgat caggttgaca ggaatcggcg tggcgctgtt cagatcgacc
      361 tgtttggcgt cgatgcgcaa ctggctcaat tcggcttcca ccggggtggg tgtggcggcg
      421 tcgcgccagc caacgttgcc gccggccacg gccagttcgc tgaccagcac cttccacggg
      481 aagtcggccg ccgggccgcc gcgcggccgg ggcttggctt tgtccttggc ggcgttgtcg
      541 ggcgcggcgg cgccgggcgg ctggcgcagc caggcctggt acatcagggc gttgtcggcg
      601 ccgcgctcca ccaccacgcg cggcgtttgc acggccagct tgccgacggt gacggcgcgg
      661 ctgcgcaggt ccaccagcag ctcggcgacg tcgatctggc cgatgctggc cagcgggccg
      721 ctgtgcttga cgccgcgcgc cgggcgtggc ggcggcgcgg cgctggcggc gggcgtgggg
      781 gcatcggcca gcatcagctg ccgcagcgtg aggctttcgg ccgccacctt caactcgggc
      841 ggcaggccct gcgcctgcgg cggcgcccac cgcaggccga cgtgggcgtc gaggtcgccc
      901 gtgagttgcg gcaccaggtg cggcgccaga tacggcgcgg ccagcgccag cggcaggccg
      961 gcgacgcgcg cgttgacctc aacctcgcgc gcggcggcgg tgccctggaa ttcgatggat
     1021 ggcacggccg agcgtgtggc cgaggcaggc gcttgcggcg cggctgcttc ggtcgctgcg
     1081 cctttgcgcg cccggcgcgg ggcggcggcg cgtggggctg ccgcgtcggg aatcgccggc
     1141 gtcagctcct cggtgccgac cagcgcggcc gagccattga agctgagcgg ttgcgaaatc
     1201 ggcaagccca tgcgcgcggc ggtcagctcg acctcgttca gccgcaccac ggcggccggc
     1261 gcgccgtcca ctgccgtggt gtcgtcgaca aaatccagcg tgccgccgtg cagcgccagc
     1321 ttgtcgatgg cgacctgcca cgcgggcgcg ggcgcggcgg cgctggcggc cgggcgcggc
     1381 gccgatgcag catcagaaat gccgccagca cttgtggggc ttgcgccagc agcttctgat
     1441 tttgtagcat cagcgccgcc aggtaccacc agcaggttga actgcccgtc cttcaggcgc
     1501 cgcagcgcca gttgcgggcc gctcagctcg accgacgaca cctgcacctt gcgctgcagc
     1561 agccgcacct cggccagctg caccttcagc gcgtcgaacg ccagcgcgtc ggcgccctgc
     1621 ccgtccaccg atttgagccc gctgacggcc tgcgcgccgc tcaggcgcag cgagggcttg
     1681 ggcgtctgct cgaagtccag cctcaagtcg gcgtcgacca ccgccgattg cagcttgatc
     1741 ggcaggctgg ccggccagta acccaggtag ggcttgaggt cgagctgggt caggcgaaag
     1801 gcggcgtcgg tctggcgcga atcggcaaac gggagcgcct cggcgcgcga atcaaacgcg
     1861 ctgccgttgg cgacaaaggc cagttgcggc tgcactttga tctgccgctc ggacggcagg
     1921 ttgctgacga agggcagcgt caggcgcaag tcgcgcacct cgtgctggcg tttcaccgcg
     1981 cggtcgtcga agtcgatgcg gccgccgctc aaatcgatgt tgtaaagcgc aaaacgcgcc
     2041 tgctcggtgg cttcgaccga ctgggggcgc gcggccagct tggccaggat gtcgtcgatg
     2101 tcgtagtggc cgtagcccag gtgcgccagg cgcagggccg gcgcctcgac ctggatggca
     2161 tcgaccaccg gcgccaggcg aaaaagcgat tgcagcgcgg cgttggcgta gatgcgcttg
     2221 acactgagct gatcgggcgc gcccgccgcg ccgcccaccg ccacgtcgtt cagcgtcagc
     2281 tccagcgtcc acggcttgaa atccaccgca ccgatgcgca ccgcgcggcc gagctgctcg
     2341 ctggcgatct tctcgccctg ccacttgagc agcggtggca ccgccagcca gcccagcgcc
     2401 cacaagccca gcagccccgc cacaccataa agcgcgcgcc gcgcccaccg attggccggc
     2461 agccagcggc gcccggcggg ggaagaaatg accgatggcg cggcagcctc ggctgcgggc
     2521 gcagtggaag tggattggga atcgccgtac atctctgacc gaattgtcgc ctcagagcgt
     2581 tcaacgtttg tatcgagacg ccgccgacac cccatcaaaa gcggccgcgc agcaggccgc
     2641 cccgaatggc cgcggagcag gccacacggg gacgccgtgg tcggggtccc ccccgaccgc
     2701 cagcgtcgtc cccctcaggg ggaaggcgcg tcagcgccac agggggagcc tagacacaag
     2761 aaagcccggc gttaaggcct tgggcctgcc cgccgggctt tcaggcagcg tgacaaaagc
     2821 gtcgtggtct gcctgggtcc gcatcggaaa gctggttgtt tccggctgcg gtgaaggcaa
     2881 gagattgcct tcgataggtg gctggtgggc tgccatgtct cggtatcgac taccagaacc
     2941 ggcccctgcc aaccgatgcc cgagtacctc ggacgccttc actgtaggcg cggccggcgt
     3001 cacgcgccag tcacgaattt caatcccgcg cccaccgggc attgcgtgcc tcaatcaacg
     3061 aaaccgcgcg ctcgcggggc cgtcgccggg cttgaaatgc gtgctcccaa ggcccgagca
     3121 gtcacgattt gtaagacttg tcttatacga aaaatagaat ttcaactcca actttcagta
     3181 ttgaccggat atgtaaccgg ctcctagaat ccgctgttcc cgaccaaaat agtgggaaac
     3241 cctgatgcac atcgatggtc aggggccgcg tgacggcgag atgccgcacc ctttgagcgc
     3301 ctgctgtcac gggcgcgcgc ggcgacagag agcgcacaca aacacaacgc gagacgtctt
     3361 tcgccagcgc agtaccccgg cccgtcgccc cgacgccgcc gacggatgct ggcccttccc
     3421 ccggacgcgc cgcgccacga ttaggagagc tatgacagcg tctggatatc ttgccgccac
     3481 ggcgcgcggc atcaagacct ttgccggtga cgtcggtgaa ggctttttcg agatcacgca
     3541 caacaccttg gccctgctgg gcattgtggt ggtggtcgtg gtggccctgc tgggcacccg
     3601 gcccgacttg cgtgcgcagg gcgaacagcg cctgaccgcc tggctgaccg cacgccaagt
     3661 ggccgccatg ggcatcacgc ccgagctggg cgccattgag cgcgccaccg ccgccaaccc
     3721 gcgcgatttg ccacgcgagc aggccaacgt ggccctgtgg atcagcaaga aataccgcgt
     3781 ggcgcccgaa cccgtcgctg cgctggtggc cgaggcttac gacgtcggcc gcgccaacaa
     3841 gctcgacccg accctgatcc tggccgtgat ggccattgaa tccagcttca acccgtttgc
     3901 gcaaagcccc gtgggcgccc aggggctgat gcaggtgatg acgcagatcc attccgacaa
     3961 gtacgagcat ttcggcggcc agtacgccgc gttcgacccc aagagcaacc tgcgcgtcgg
     4021 cgtgaaggtg ctgagcgaat acgtcacccg caccggctcg gtcgaaggcg gcctgaagtg
     4081 gtacgtgggc gccgccaacc tgcccaccga cggcggctac gccgacaagg tgatggccga
     4141 gcacagccgc ttgagccacg tcgccaaggg caagactatc ccgctgaccg acaacacgcc
     4201 ggccctgcgc gcggccacca cgccggcgcc gtctgctgcg ccggctggca acgcgccggt
     4261 caaggtcgcc gcgctgtcct gaacggcacg gcgcgggccg gcgccgcttt ggccgtcggc
     4321 taaactcacc gggttgcgcg actggcgata agcacccctt gacggcggtg ccgacgtggg
     4381 ccaccacggg gaagcgcacc gcccgctgca tgcaacgggt gatcagccgt tcgcctgggc
     4441 agcccttgca cttcaaggga tcaccttctc gaaggactgc catgttcaac cgttccacgc
     4501 acaccattgc cgccaccgac gccgacgtct gggccgccat ccaggccgag caccagcgcc
     4561 aggaagacca catcgaactg atcgcgagcg aaaactacgc ctcgcccgcc gtgctggccg
     4621 ccgccggcac gcagctgacc aacaaatacg ccgagggcta ccccggccgc cgctactacg
     4681 gcggctgcga atacgtcgac gtggtcgagc agctggccat cgaccgcgtc aagcagctgt
     4741 tcggctcgca agccgccaac gtgcagccgc actgcggcgc cagcgccaac gaggcggtgt
     4801 tcctggcctt cttgaagccc ggcgacacca tcatgggcat gagcctggcc gaaggcggcc
     4861 acctgacgca cggcatgccg ctcaacatca gcggcaagtg gttcaacgtg gtgagctacg
     4921 ggctcaacgc gcaggaagcg ctcgactacg acgcgatgga gaaaaaggcg cacgaaacca
     4981 agcccaaact catcatcgcg ggcgccagcg cttactcttt agctatcgat tttgagcgct
     5041 ttgccaaggt cgccaaggac gtgggcgcga tcttcatggt cgacatggcg cactacgccg
     5101 gcctgatcgc cggtggccaa taccccaacc ccgtgccgca cgccgacgtg gtgaccagca
     5161 ccacgcacaa gagcctgcgc ggcccgcgct cgggcttcat tttgatgaag tcgcagcacg
     5221 aaaaagccat caactccgcc gtcttccccg gcctgcaggg cggcccgctg atgcacatca
     5281 tcgcggccaa ggcgattgcc ttcaaggaag cgttgcaacc cgaattcaag cagtacgcgc
     5341 agcaggtcaa gaagaacgcc caggtggtgg ccgagacgct gacgcagcgc ggcctgcgca
     5401 tcgtgagcgg cggcaccgaa agccacgtca tgctggtcga tctgcgcgcc aagggcatca
     5461 ccggcaagga agccgaggcc gtgctgggca gcgcccacat gacgatcaac aagaacgcca
     5521 tccccaacga cccggaaaag cccatggtga ccagcggcgt gcgcatcggc acccccgcca
     5581 tgaccacgcg cggctttggc gagaccgaag cgcgagccac cgccaacctg gtggccgacg
     5641 tgctcgacaa cccgggcgtc gaggccaacc tgaacaccgt gcgcgccaag gtggccgagc
     5701 tgaccaagcg ctttccggtg tacggcggtt gatgggaact actgaattga tagctgcttt
     5761 cgcttgtcag acaagcgctg gcggcctatt tgattcatag cctgcatgcg ctgcccgttc
     5821 tgcgcccaca ccgaaacgca ggtcgtcgac acccgcgttt cggacgaggg cgacttcatc
     5881 cgccgccgcc gccagtgcgg ccactgcgcc aagcgcttca ccacctacga gcggccggac
     5941 gtcagctttc ccagtatcgt caagaaggac ggccgccgcg tgccctactc gaagcccaag
     6001 ctgcaaggct cgcttgacct ggcgctgcgc aaacgcccgg tgagcaccga gcagatcgac
     6061 gccgccatcg agcgcatcga agaaaagctg ctcagcgccg gcgcacgcga agtgccttcc
     6121 agccgcttgg gcgagctggt gatgcgcgag ctgaagaagc tcgacaaggt cgcctacgtg
     6181 cgctttgcca gcgtctaccg cagcttcgag gacatcgacg aattccgcgc gctggtggac
     6241 gaagtgcggc gctgatcgcc ccgcccgttt gcgcgcaggc gctgcgtttt ttgggcgatg
     6301 atttccgcca tgccgaccgt gcccgccagc cactttgtgc cgcccgtgcg cgggtggcca
     6361 ctgggcgcgg cggtgctggc gtcggtgctg ctgcacctgg ccgcctggtt ctggattcag
     6421 ccgacgcgcg tggcgccgcg cgcgggcgga gcagaaaagc cgatgcgcat cagccttcac
     6481 acgctgccgc cgcgcccggc agcgcccgtt cctgcgccac cgctcccccg cgccacgcca
     6541 cgtgcgcaga cccaagcggc gccgcccgcg ccgcgcgccg acgtgcgcca gtcaccgcct
     6601 gagatcgcgc ccccgctggc cgcagagacg ccgcccgagc cgccagcgcc ggccggcgcg
     6661 gccacgccgt ccaacatgct ctacgtgccc gccgcgctgg ccgatcaagc cccggtgccg
     6721 ctgggcgact gggtgctggc cgaggaaccc tggcccgacg gcgtgggcgc cgtgcagatc
     6781 acgctctgga tcgacgcaga aggccgtatc gagcgcacgc tgctgctcgg cgcggcggcc
     6841 gacgatccgc gcgtgcaggc gctgtttgcg cgcattgccg acacgccgat gcagccggcg
     6901 cgcgtcggca ccggcgacgt gcacagcgtg atgacggtgg aaatctggga tgaaacgaca
     6961 agcggctcca ccgtctgggg cacggcgcct gcggacgcgg cgagcggcag tgccagaaca
     7021 ctatcgaaca gatagctgct ggcgcttgat ggacaagcgc tggcgaccga tttgacctga
     7081 aacccctgat tcaggctgct tcaaaaaaca gccggacgca gagatttcgc agaggacgca
     7141 aaaaagtcaa agaacacctt tttctggctg ttctttcgcg tcctctgcga atcctttgcg
     7201 tcctctgcgt ccggtcttcg gttttcggtt tccgcagcgc cccaaaaaac tggcgcccaa
     7261 ggcgccagtt ttgtcagaga ccgtgggcca ccctcagcgc ggcaggtgcg ccatcaccca
     7321 ctgcacgatc tgcgccgggt tggtcatggc gcccggctgg cgcgccactt ccttgccgcc
     7381 gacaaacagc gccagcgtcg ggatgctgcg gatgttgtag cgcccgccca gcgcctgcgc
     7441 ggcctcggtg ttgaccttgg ccacgcgcac ctgcggctcc aggtcggccg cggccttctc
     7501 gtaagccggc gccatcatgc ggcacggccc gcaccacggc gcccagaagt cgaccagtac
     7561 ggggatctgg ctgcgcagca cgtgcttgtc gaacgccgct tcatcgtcca gcgcgaccgg
     7621 gtggccggtg aacagcggct gatgacactg gccgcagtcg gggtggctcg ccagttgatc
     7681 gggcttgacg cggttggtgg tgctgcagtg cgggcagacg atgtgcaggg gttcgctcac
     7741 aaatcgctcc ttgggtcgcg gtggatcagg ccgccggcgc tgcgtcgggc gccggcttgc
     7801 tgcgcacggc gtcgatcagc gcgcgcgccg cgacctcggc gcgcgtggcg gcttcgagcg
     7861 gcgagcggca caggccggcc tcggcgtagt gcaggttttc atagatggcc gcagccaccg
     7921 cgctttcgtc cagcagcggc tgccagtcga catcggggtc ggccagcatg tcggacaaac
     7981 gggccaccac ggtgcggtac tggtgcgcat cgaccgacgt gcggctggca tccagccgct
     8041 ccagcagctg cgccagcacg gccgcggtcc gagaggggga ttgcaaggtt ttcatggctc
     8101 gcaaatgggg gtcggtggcc tcaattgcaa ggtgttcggg ccagcgcagg aacactacgc
     8161 ttttcatagc ttcttgcgca cgctggacag gcgccagggc ctgaaaacac catcaaccaa
     8221 tcaagggcgc cgcagcggat cgagcagccg ccgcgcgccc gcctgatcga cctcgtacat
     8281 cagcgccagc aagcgcccga gctgcccctt ggcaaagccc ttttgcgcga accagcccag
     8341 gtagtggctg ggcagatcgg ccagcagcgt gcccttgtac ttgccgaagg gcatggccca
     8401 gtcgaccagc aattgcaggt cggccgccgc gtgcggctcg cgcgctgcgt cgttcacgct
     8461 ggcgttggat cagcccagcg ccttggccag ccggccgatg ccttcccgaa tcttgccttc
     8521 gtccgccgtg gcaaacgaca ggcgcagcgt gctgagatca gggttggcgc aatagaacgg
     8581 cgcgccgggc acgaaggcca cgccttcggc aatggcgcgc ttggcaaatt cgttgccgtc
     8641 cttgatcttg ccgcccgcgc ccgtgagccg cgcccacacg aacaatccgc cctgcggctg
     8701 cacgaagtca atctccgcac ccaggtcatt gcgcagcgcg tcgcccatgg tcttgccgcg
     8761 cgcgccatac acctcgcgca cgcgcgccag cgtgccgggc atgtggccgc cggccagata
     8821 ctgcgcggcg gtggcctgcg cgaaggtgct ggtgtgcgcg tcgctgaact gcttgcacat
     8881 ggtcgccttg gccagcaact cgggcggcgc caccatccag cccacgcgca ggccgggcga
     8941 caacaccttg ctcaagctgc cgcagtgcac cagccagtcg cggctgccgg gcacctgcgc
     9001 cgacagcgcc agcaggctgg gcggcggcgg cgcgtcgaag tacaaatcgc cgtacggatc
     9061 gtcttcgatg atcagcgtct ggtacttcac cgccagctcc agcacgcgct tgcggcgctc
     9121 caggctcagc atggcgccgc tcgggttgcc aaaggtggga atcaggtaga cgaacttggg
     9181 cttgtgttcg gtgatcagcc gctccagctc atcgacctgc acgccctcgc cgtcgatcgg
     9241 cgcgctgatc agctcggcgc cgtacaagcg aaagcactgg atggtggcca gaaacgtcgg
     9301 cccttccaca atggccttgc tgtccggatc gaccaggcac ttgcccagca aatccagcgc
     9361 ctgctggctg ccggtggtga cgatgagctg gtcggccgcc acctgggctc ccttggcttg
     9421 catgaacgcc gccagctgct cgcgcagcgg ctggtagccc tcggtggcgc cgtactgcag
     9481 cgcggcgccg ggctcgtcga tcagcgcctt gttcaccgcc tcagtgatgc cgtgcacgtc
     9541 gaacatcgcg ctgtccggaa agccgcccgc aaagctgatg atgccgggct tgcccagcag
     9601 cttgaacagc tcgcggatgg cggaggtttc gacgttatcg aggcgttggg caaaggggaa
     9661 ggtcatggct tggctcgggt tgatccagcg cctattttcg ccaatcgcac gcttacactg
     9721 ccccgcgcat gacccccgcg caaatcgaga ccttcttcgc caccctgcaa gccatcaatc
     9781 cgctgccggc cagcgagctg gaatacacca gcgtgttcga gctgctggcg gccgtgctgc
     9841 tgtcggcgca ggccaccgac gtgggcgtga acaaggcgac gcgccggctg tttccggtgg
     9901 cgaacacgcc gcagcaaatc atcgatctgg ggctggagcg cctggagggc tacatcaaga
     9961 ccatcggcct gtaccgcagc aaagccaagc acctgatgga aacctgccgc atcctggtcg
    10021 agcgccacgg cggccaggtg ccgcgcacgc gcgccgagct ggaggcgctt cccggcgt
//
LOCUS       DAOMVO010000059         9956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1685_length_9956_cov_12.555600, whole genome shotgun sequence.
ACCESSION   DAOMVO010000059 DAOMVO010000000
VERSION     DAOMVO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_9956_cov_12.555600"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKV30_04635"
     CDS             <1..440
                     /locus_tag="PKV30_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRL29539.1"
                     /translation="GGAAFIDIISPCVTFNNHAGSTRSYDWVREHNEAVSRIDFITER
                     EEITATIQPGEVVDVEQHDGTTLRLRKLHTDYDPTDRVVAMSYMHARAAEGELLTGLL
                     YVEGEAGDLHTALNTSARPLNSLGAADLCPGTGALDKINASLR"
     gene            complement(455..1108)
                     /gene="rsxB"
                     /locus_tag="PKV30_04640"
     CDS             complement(455..1108)
                     /gene="rsxB"
                     /locus_tag="PKV30_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HRL29540.1"
                     /translation="MGATTDQLLAALPQTQCTRCGYPDCAAYANALAGGDAAINQCPP
                     GGAEGVARLAAITGHAVIPLNPINGSEGPRHAAWIDENWCIGCTLCIKACPVDCIVGG
                     NKRMHTVIEAQCTGCELCIPVCPVDCIALENVTGERTGWAAWSAKDADSARARYEFTS
                     WRHIRDARRHTETLEQKARDKLARLPELTKNASGPELDRKRAIIEAALARARAQRGD"
     gene            complement(1176..2768)
                     /gene="phaZ"
                     /locus_tag="PKV30_04645"
     CDS             complement(1176..2768)
                     /gene="phaZ"
                     /locus_tag="PKV30_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HRL29541.1"
                     /translation="MLYQIYEAQRSLLEPFADMADAASKLYGNRHTLLGQMPMAQRVS
                     AAYALFHRLGKDYEKPEFGIRKVDVDGVEVAIDERVELDMPFCQLLRFKRFSDDPATL
                     EKLKGQPPVLIVAPLSGHYATLLRDTVRTMLEGHKVYITDWKNARMVPLSEGEFHLDD
                     YVNYVQEFIRHLQGKYGNCHIISVCQPTVPVLAAVSLMASRGEQTPITMTMMGGPIDA
                     RKSPTAVNNLATQRSYEWFENNVIFRVPQNYPGAGRRVYPGFLQHTGFIAMNPDRHAS
                     SHYDYFKDLIKGDQSSAETHRKFYDEYNAVLDMDADYYLETIKTVFQDFKLVNGTWDV
                     RSPEGKLERVRPQDITATAQMTVEGELDDISGSGQTKAAHALCKGVERREGDHLEVKG
                     AGHYGIFSGRRWRTVVYPQVKAFIADYHAKAVAKASEPASAAASPSQVAKPQQRAADA
                     LGQLRLDDLGDVAPDLAKAPAPQRTPVKRAAAKKVAAPRRKAAAAPARLAAKAPVSAA
                     AKRAAPGARATTPRSNGRARQS"
     gene            2921..3724
                     /locus_tag="PKV30_04650"
     CDS             2921..3724
                     /locus_tag="PKV30_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL29542.1"
                     /translation="MTTGFSLYGWLIGLGQGVLRWLFGWWTVVFIGAQLAVLAFSPSS
                     YGAEARGAVLRQIYRAAGPGLPGFTTLMALFNVVLIRILVVTAFAYGLTQYALDAVVR
                     VLVLELIPLIAALFVAVEYTIPGGSELYKLRRSGALQALRERGVEPLSHEVLPRVLAG
                     IFAVLLLAALSCFLSLVLAYIAVHGFTLAGVPSYNHAVGNIFNPVVSLIFSMKTLLFA
                     IAVALLPVANALRDIPRRASRTSVELQGLVQMFVLMLVIEIASLIGNYY"
     gene            3732..4805
                     /locus_tag="PKV30_04655"
     CDS             3732..4805
                     /locus_tag="PKV30_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRL29543.1"
                     /translation="MTTPQDPTPPALPEPQAPPLPGDGEPADARGELPPASRRSQRAL
                     EFRAALLLILMALLIGASVLYLMWVRGAFEATQKLYLAADDSEGVVVGMDMTFSGFPI
                     GRVSRIELVEAGTVRIHVDVPVRSAHWLRTSSVFTLEKGLVGASRLRAFTGVLDDPPL
                     PPGSERIVLRGDVSAEIPKMVADARDVLQNLNRLTEAESALAGTLTEIRTFTGRLNSA
                     KGGLMAAVTGNEADSKRVAELLDRTNQLVKNLDTVAVRVDGVVKKADTQVLGKDGLVT
                     DAQAAVRQLNTLLQDVRQTVVKIDGVLKEVQGVASNANQATTNLGDLRADVESSLLKI
                     DALITELNRKWPFAPKEKEVKLP"
     gene            5051..5674
                     /locus_tag="PKV30_04660"
     CDS             5051..5674
                     /locus_tag="PKV30_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29544.1"
                     /translation="MIAGCESGPKVPDWSINAVGHAERFTEAYFTGNTRIEAREFTLA
                     RAEVARTGRADLVARIELTRCAARVASLEFGPCEGFEPLRADASDAERAYAHYLAGQV
                     SAQDAALLPPQHRAIAAGGASVAGIEDPAARLVAAGVLLRQSRATPDTAAQAVETASH
                     QGWRRPLLAWLGVQARLADERGDAAEAARIRRRMDLVQPAVQITPRP"
     gene            complement(5948..7078)
                     /locus_tag="PKV30_04665"
     CDS             complement(5948..7078)
                     /locus_tag="PKV30_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HRL29545.1"
                     /translation="MTVQSHINRPLGEHASTLFLSVPHAVELVRRKGLATCLRGIAPA
                     IRDDFLRWPEFDKTARVAAHSAHSAQGVIELIPVADGERYSFKYVNGHPYNHRLGLPT
                     VMAFGVLAEVANGRPLLLSELTLTTALRTAATSALAAQALARPGSRVMALIGNGAQSE
                     FQALAFQALVGIQALRVYDVDDAATDKLVANLHDSGLTITRCASTAEAVRGADIVTTV
                     TADKLHATVLTADMLAPGMHLNAVGGDCPGKTELAADVLSAARVFVEYPPQTRVEGDI
                     QQMPADFAVTELWQVLAGKQPGRVAAEDITEFDSVGFALEDYSALRFMHAAALELGLG
                     ERIALVPEMDDPKDLMGLLRLPATPDRHSPHQPLATRAAATA"
     gene            7093..7464
                     /locus_tag="PKV30_04670"
     CDS             7093..7464
                     /locus_tag="PKV30_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL29546.1"
                     /translation="MTAVADLARKLAVSRGTVTNRVQRLQDAQVIVGYTVRLKPDAEP
                     ERIRAWMGITVEGNQSRAVIASLLGEPGVSALHDTNGRWDLLAELQTDSMSGLSQVLE
                     RIRLIKGIRGTTTSIHLASYR"
     gene            complement(7455..8504)
                     /gene="rsgA"
                     /locus_tag="PKV30_04675"
     CDS             complement(7455..8504)
                     /gene="rsgA"
                     /locus_tag="PKV30_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRL29547.1"
                     /translation="MSRSRRGAPLTHDASLREGLVVGAHGRHCVVETPAGERVICHPR
                     GKKLAVVVGDRVRWLPSQDEGTIERIDERRNLLYRQDEVRTKSFAANLDQVLILVAAE
                     PEFSEHQLSRALIACEAEHITPLIVLNKADLAAPFARAWQRLAPYRAMGYTVLPLAAK
                     PVLPPLPLGAGWVEGDPAPLATPPHPHPNPLPPAGEGAMLADVTERMHCHTTLILGPS
                     GAGKSTLINRLIPGAAAQTGEISQALNSGKHTTTTTTWYWLDHEKTSALIDSPGFQEF
                     GLRHIDPMQLAAYMPDIKPHVAECKFYNCTHLHEPGCGVISNVESTVGAGGISASRYK
                     IYTDLFGELRAAPQR"
     gene            complement(8624..9928)
                     /locus_tag="PKV30_04680"
     CDS             complement(8624..9928)
                     /locus_tag="PKV30_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRL29548.1"
                     /translation="MNESLSPALVTSLVFSLALLANVAVKFWLASRQIRAVARHRAAV
                     PAEFVGTIALDAHQRAADYTVAKLRFGLLELALASAVLLGWTLLGGLDWLNNALMDAV
                     GPRPLLQPLALLVAFAVINALIDLPFSLYQTFVIEERFGFNKMTLRLWLGDLIKSTLV
                     GTAIGVPLAALVLWLMQATGAWWWLWTWGAWAAFSLLMLVIYPTVIAPLFNKFQPLQD
                     ESLKARVSALMARCGFAAKGLFVMDGSRRSAHANAYFTGFGAAKRVVFFDTLLEQLTP
                     PEVEAVLAHELGHFKHKHIVKRIAGLFVASLAGFALLGWLAQQPWFYAGLGVAPNLPP
                     AMGGAVPNHALALLLFMLAVPLFTFFLSPLMAQLSRKHEFEADAYAARQANAQDLSNA
                     LVKLYKDNASTLTPDPLYVRFYYSHPPASERIARMGARVQPA"
BASE COUNT         1601 a         3249 c         3437 g         1669 t
ORIGIN      
        1 acggcggcgc ggccttcatc gacatcatca gcccctgcgt caccttcaac aaccacgccg
       61 gcagcacgcg cagctacgac tgggtgcgcg agcacaacga agcggtgagc cgcatcgact
      121 tcatcaccga gcgcgaggaa atcaccgcca ccattcagcc cggcgaagtg gtcgatgtcg
      181 agcagcacga cggcaccacg ctgcgcctgc gcaagctgca caccgactac gacccgaccg
      241 accgcgtggt ggcgatgtcg tacatgcacg cccgtgcggc cgaaggcgag ttgctgacgg
      301 gtctgctgta cgtggaaggc gaggcaggcg acctgcacac ggcgctgaac accagcgcgc
      361 ggccgctgaa cagcctgggc gcggccgacc tgtgcccagg cacgggggcg ctggacaaga
      421 tcaacgccag cctgcgctga cgcggcgcgc gacttcagtc gccgcgctgg gcgcgcgccc
      481 gcgccaatgc cgcttcgatg atggcgcgct tgcggtcgag ttcggggccg ctggcgttct
      541 tggtcaactc gggcaggcga gccagcttgt ccctggcctt ttgttcaagc gtttcagtgt
      601 gccggcgcgc gtcccgtatg tgccgccagc tcgtaaattc atagcgcgcg cgggcactgt
      661 cggcgtcttt cgctgaccag gccgcccagc cggtgcgctc gccggtgacg ttttccagcg
      721 cgatgcaatc gaccgggcag accggaatgc acagttcaca gcccgtgcat tgcgcttcga
      781 tgactgtgtg catccgcttg ttgccgccca cgatgcaatc gaccgggcag gccttgatgc
      841 acaaggtgca gccgatgcac cagttctcgt cgatccaggc cgcatggcgc ggcccttcgc
      901 tgccgttgat cgggttgagc ggaatgacgg catgccccgt gatggccgcc aggcgcgcca
      961 cgccctcggc gccaccgggc gggcattggt tgatggccgc gtcgccgccc gccaaggcgt
     1021 ttgcgtaggc cgcgcaatcg gggtagccgc agcgcgtgca ctgcgtctgc ggcaacgcgg
     1081 ccagcagttg atcggtcgtc gcgcccatgc cttgtcaacg cctgaaaaaa caacgcccgc
     1141 caaggcgggc gcggtggctg atcatcgccc ggccttcagc tttggcgtgc gcggccattg
     1201 ctacgggggg tggtggcgcg tgcgccgggt gcggcacgct tggcggcggc gctgacgggc
     1261 gccttggctg caagcctggc cggagccgca gcggccttgc ggcgcggtgc agccactttc
     1321 ttggctgcag cgcgcttgac cggcgtgcgc tggggcgcgg gcgccttggc caaatcaggc
     1381 gccacatcgc ccagatcgtc cagccgcagc tggcccagcg catcagccgc tcgttgctgc
     1441 ggcttggcga cttgcgatgg actggcggcg gcgctggccg gctcgctggc cttggccacc
     1501 gccttggcgt ggtagtcggc gatgaaggcc ttgacctgcg ggtacaccac ggtgcgccag
     1561 cggcgcccgc tgaagatgcc gtaatgcccg gcacccttga cctccaggtg atcgccttcg
     1621 cggcgctcga cgcccttgca cagcgcgtgc gcggctttgg tctggccgga gccggagatg
     1681 tcgtccagct cgccctcaac ggtcatctga gcggtggcgg tgatgtcttg cgggcgcacg
     1741 cgctcaagtt tgccctcggg cgagcgcacg tcccaagtgc cgttgaccag cttgaagtcc
     1801 tggaacacgg tcttgatcgt ttccaggtag tagtccgcgt ccatgtccag cacggcgttg
     1861 tattcgtcgt agaacttgcg gtgcgtctcg gcgctggact gatcgccctt gatcaggtcc
     1921 ttgaagtagt cgtaatggct gctggcgtgc cggtccgggt tcatggcgat gaagccggtg
     1981 tgctgcagaa aaccggggta gacgcggcgg ccggcgcccg ggtagttctg cggcacgcgg
     2041 aagatcacgt tgttctcgaa ccattcgtag ctgcgctgcg tggccaggtt gttgacggca
     2101 gtcggcgact tgcgcgcgtc gatggggccg cccatcatgg tcatggtgat cggcgtctgc
     2161 tcgccgcggc tggccatcag cgacacggcg gccagcaccg gcaccgtcgg ctggcacacg
     2221 ctgatgatgt ggcagttgcc gtacttgccc tgcaggtggc gaatgaactc ctgcacgtag
     2281 ttgacgtagt cgtccaggtg gaattcaccc tctgacagcg gcaccatgcg ggcgttcttc
     2341 cagtcggtga tgtagacctt gtggccttcg agcatggtgc gcacggtgtc gcgcagcaac
     2401 gtggcgtagt ggcccgacag cggcgccacg atcagcaccg gcggctggcc cttgagcttt
     2461 tccagcgtgg ccggatcgtc ggagaagcgc ttgaagcgca gcagctggca aaacggcatg
     2521 tccagctcga cgcgctcgtc aatggcgact tcgacgccgt cgacatcgac cttgcggatg
     2581 ccgaactcgg gcttctcgta atccttgccc agccggtgaa acagcgcgta ggcggccgac
     2641 acgcgctgcg ccatcggcat ctggcccagc agcgtgtgcc gattgccgta cagcttggaa
     2701 gccgcatccg ccatgtccgc gaacggctcc agcagggagc gttgcgcttc gtagatttga
     2761 tagagcatga gaggttcggt gacgaagtgt tgcagtgcaa tataacgcag cgtcggtcga
     2821 aaagcagtgg gattaatcct ctaaagcgcg tccgtgacac cttggcgact gtccgcgagg
     2881 gccgcggtat cgcctggccc cacgggtatc atgccgcgcc atgaccaccg ggttttcgct
     2941 ctacggctgg ctgatcgggc tggggcaggg cgtgctgcgc tggttgttcg gctggtggac
     3001 ggtggtgttc atcggcgcgc aactggcggt gctggcgttt tcgccgtcca gctacggtgc
     3061 cgaggcgcgc ggcgccgtgc tgcgccagat ctaccgcgcc gccgggccgg gcctgccggg
     3121 tttcaccacg ctgatggcct tgttcaacgt ggtgctgatc cgcatcctgg tggtgaccgc
     3181 ctttgcctat ggcctgaccc aatacgcgct ggacgccgtg gtgcgcgtgc tggtgctgga
     3241 gctgattccg ctcatcgcgg cgctgttcgt ggcggttgaa tacaccattc ccggcggttc
     3301 cgagctgtac aagctgcgcc gcagcggcgc gctgcaagcc ctgcgcgagc gcggcgtcga
     3361 gccgctcagc cacgaagtgc tgccgcgcgt gctggccggc atctttgccg tgctgctgct
     3421 ggcggcgctc agctgctttt tgtcgctggt gctggcctac atcgcggtgc acggcttcac
     3481 gctggccggc gtgccctcgt acaaccacgc ggtgggcaac atcttcaacc cggtggtgtc
     3541 gctgatcttc agcatgaaga cgctgctgtt cgccattgcc gtggcgctgc tgccggtggc
     3601 caacgcgctg cgcgacattc cgcgccgcgc ctcacgcacc agcgtcgagc tgcagggcct
     3661 ggtgcagatg ttcgtgctga tgctggtgat tgagattgcg tcgctgatcg gcaattacta
     3721 ttgaagcccc gatgacgacg ccgcaagacc ccacgccgcc cgcgctgccc gaaccgcaag
     3781 cgccaccgtt gccgggcgac ggcgaaccgg ctgacgcgcg gggcgagctg ccgcccgcca
     3841 gccgccgatc acagcgcgcg ctggaatttc gcgccgcgct gttgctgatc ctgatggcgc
     3901 tgctcatcgg cgcctcggtg ctgtacctga tgtgggtgcg cggcgccttc gaggcgacgc
     3961 aaaagctcta cctcgcggcg gacgattccg aaggcgtggt ggtcggcatg gacatgacct
     4021 tttccggctt tcccatcggt cgtgtcagcc gtattgagct ggtcgaggct ggcacggtgc
     4081 gcatccacgt cgatgtgccg gtgaggtcgg cgcactggct gcgtacgtcc agcgtgttca
     4141 cgctcgaaaa aggcctcgtc ggcgcctcgc gcctgcgcgc cttcaccggc gtgctggacg
     4201 acccgccgct gccgcccggc agcgaacgca tcgtgctgcg cggcgatgtg tcggccgaaa
     4261 tccccaagat ggtggccgac gcgcgcgacg tgctgcaaaa cctgaaccgc ctgaccgaag
     4321 ccgaatcggc cctggccggc acgctgaccg aaatccgtac cttcaccggc cggctcaaca
     4381 gcgccaaggg cgggctgatg gcggccgtca ccggcaacga ggccgattcg aaacgcgtgg
     4441 ccgagctgct agaccgcacc aatcagctgg taaaaaacct cgacaccgtg gccgtacgcg
     4501 tggacggcgt ggtcaagaag gccgacaccc aggtgctggg caaggacgga ctggtcaccg
     4561 acgcccaggc cgccgtgcgc cagctgaaca cgctgctgca ggacgtgcgg cagacggtgg
     4621 tcaagatcga cggcgtgctg aaggaagtgc agggcgtggc cagcaacgcc aaccaggcca
     4681 cgaccaacct gggcgacctg cgtgccgacg tcgagagcag cctgctcaag atcgacgcgc
     4741 tgatcaccga gctgaaccgc aaatggccct tcgcgccgaa agaaaaagag gtcaagctgc
     4801 cgtgagaaag ctccccctgt gccggcctgc ggccgtcttc ccccgcaggg ggacgcttgc
     4861 agcggccggg ccaagccagc tccgcgcaag ccccgaacgg cgcgctggca cggcgcctga
     4921 tttttctccc tcgcccctct ggggagggtg caggggtgag gggttggcgc atcctcgtca
     4981 atccaagtca aatagccgcc cagcgcttgt ctggaaagcg caagcagctc ttttattggt
     5041 agcgtgcgcc ttgatcgctg gctgcgaaag cggccccaag gtgcccgact ggagcatcaa
     5101 cgccgtcggc catgccgagc gctttaccga agcctatttc accggcaaca cgcgcatcga
     5161 ggcgcgcgaa ttcacgctgg cgcgcgcgga agtcgcccgc accggccgcg ccgatctggt
     5221 ggcgcgcatc gagttgaccc gctgcgccgc gcgcgtggcc agcctggagt ttggtccctg
     5281 cgaaggcttc gagccacttc gcgccgacgc cagcgacgcc gagcgtgcct acgcccacta
     5341 cctggccggc caggtcagcg cgcaagacgc cgcgctgctg ccgccgcagc accgcgcgat
     5401 tgctgcgggc ggcgccagcg tggccggcat cgaagatccc gccgcccgcc tggtcgccgc
     5461 cggcgtgctg ctgcgccaaa gccgcgccac gcccgacacc gccgcgcaag cggttgaaac
     5521 cgcatcgcac cagggctggc gccgcccact gctggcgtgg ctgggcgtgc aggcgcggct
     5581 ggcggatgag cgcggcgatg cggccgaagc ggcgcggatc aggcggcgga tggatttggt
     5641 gcagcctgct gttcagatca cgccacgccc ttgacgcggt gcgcgccacg gcgagcgcac
     5701 tgaaacgtca ggcgtttttg ctgcaagttt gagagctgct cgcgcttgcc aatcctgcgc
     5761 cagcggctga tttgatctgt caacgtcagc ccgtaggccg ggtagaacga agtgaaaccc
     5821 agcggtgacg cggtgactca agctgtgttt cgctgcgctc tacccaggct aaatgaagtg
     5881 caaatttgag agctgcttgc gctggccccg cgtgcgcaag cggctgattt gatcggaata
     5941 cctcaaatca tgcggttgcc gcggcgcgcg tcgccaacgg ctggtgcggc gaatgccgat
     6001 ccggcgtggc cggcagccgc agcagcccca tcaaatcctt cggatcgtcc atctccggca
     6061 ccagcgcgat gcgctcgccc aaacccagct ccagcgcggc ggcgtgcatg aagcgcaggg
     6121 cggaatagtc ttcgagcgcg aagccgaccg aatcgaactc ggtgatgtct tcggccgcga
     6181 cgcgccccgg ctgcttgccg gccagcacct gccacagctc ggtgacggca aagtcggccg
     6241 gcatctgctg aatgtcgccc tcgacgcgcg tttgcggcgg gtattcgaca aacacgcggg
     6301 cggcgctcaa cacatcggcc gccaattccg tcttgcccgg acagtccccg cccacggcgt
     6361 tgaggtgcat gccgggcgcc agcatgtcgg ccgtcaggac ggtggcgtgc agcttgtcgg
     6421 cggtgacggt ggtcacgatg tcggcgccgc gaaccgcttc ggctgtgctg gcgcatcggg
     6481 tgatcgtcaa accgctgtcg tgcaggttgg cgacgagctt gtcggtggct gcatcgtcca
     6541 cgtcgtacac gcgcagcgcc tggatgccga ccagcgcctg aaacgccaac gcctggaatt
     6601 cgctttgcgc gccgttgccg atcagcgcca tcacgcggct gccgggccgt gccagcgcct
     6661 gcgcggccag ggccgaagtg gcggcggtgc gcagggcggt ggtcagcgtc agctcggaca
     6721 gaagcagcgg ccggccgttg gcgacttcgg ccagcacgcc aaaggccatc acggtgggca
     6781 gacccaggcg gtggttgtaa gggtggccgt tgacgtactt gaagctgtag cgctcgccat
     6841 cggccacggg tatcaactcg atcacgccct gcgccgaatg cgccgaatgc gccgccacgc
     6901 gcgcggtctt gtcgaattcg ggccagcgca gaaagtcgtc gcgaatggct ggtgcgatgc
     6961 cgcgcaggca agtggccagg cccttgcggc gcaccagctc aacggcgtgc ggcacgctca
     7021 ggaacaaggt gctggcgtgc tcgccgaggg ggcggttgat atggctttgg acggtcatga
     7081 cagccagttt caatgaccgc agtggccgat ctggcgcgca agctggcggt gtcgcgcggc
     7141 accgtcacca accgcgtgca gcggctgcag gacgcgcagg tgatcgtcgg ctacaccgtg
     7201 cggctgaagc ccgacgccga gcccgagcgc attcgcgcct ggatgggcat cacggtggag
     7261 ggcaaccaga gccgcgcggt gatcgccagc ctgctgggcg agccgggcgt gtcggcgctg
     7321 cacgacacca acggccgctg ggatttgctg gccgagttgc agaccgattc gatgagcggc
     7381 ctgtcgcagg tgctggagcg gattcggctg atcaagggca ttcgcggtac cacgaccagc
     7441 attcacctgg ccagctaccg ctgaggggcg gcgcgcagtt cgccaaacag gtcggtatag
     7501 attttgtagc gactcgcgct gattccgcct gcgccaacgg tcgattcgac gttggagatc
     7561 acgccgcagc ccggctcgtg caggtgcgtg cagttgtaga acttgcactc ggccacgtgc
     7621 ggcttgatgt cgggcatgta ggcggccagc tgcatcgggt cgatgtggcg caaaccgaat
     7681 tcctgaaaac cgggcgagtc gatcagggcg ctggttttct cgtggtccaa ccagtaccac
     7741 gtcgttgtcg tggtggtgtg tttgcccgag ttgagcgctt gcgaaatttc gcccgtttgc
     7801 gcggcggcgc cggggatcag gcggttgatc agcgtgcttt tgccggcgcc ggaggggccc
     7861 aggatcagcg tggtgtggca gtgcatgcgc tcagtcacgt cagcgagcat tgcgccctct
     7921 cccgctggcg ggagagggtt ggggtgaggg tgaggcggcg tggccaacgg agcggggtcg
     7981 ccctcaaccc agcccgctcc caaggggaga gggggcaaga ccggcttggc ggccagcggc
     8041 agcacggtgt agcccatggc gcggtacggc gccagccgtt gccacgcgcg cgcaaacggc
     8101 gcggccaggt cggccttgtt cagcacgatg agcggcgtga tgtgctcggc ctcgcaggcg
     8161 atcagcgcgc gggacagctg gtgctcagag aactccggct cggcggcgac caggatcagc
     8221 acctgatcca ggttggcggc aaacgacttg gtgcgcacct cgtcctgccg atagagcagg
     8281 ttgcggcgct cgtcgatgcg ctcgatggtg ccctcgtcct gagatggcag ccagcgcacg
     8341 cggtcgccga ccaccacggc cagcttcttg ccgcgcgggt ggcaaatgac gcgctcgccc
     8401 gccggcgttt cgacgacgca atgacggccg tgcgcgccga cgaccaggcc ttcgcgcagg
     8461 ctggcgtcgt gggtgagcgg cgcgccgcgg cgggatcggc tcaagacagc accgctgagg
     8521 attggtgcag cgtgcgctgg atgactgcaa aatcaatagc tgctggcgct tgttgctcaa
     8581 gcgcttgcgg ctgatttgat tcaaatttca tgcggcgcca ccgtcaagcc ggttgcacgc
     8641 gcgcacccat gcgcgcgatg cgttcgctgg cgggcgggtg cgagtagtag aagcgcacgt
     8701 acagcgggtc gggcgtcagc gtgctggcgt tgtccttgta cagcttgacc agcgcgttgc
     8761 tcaggtcttg cgcgttggcc tggcgcgccg cgtaggcgtc ggcttcaaac tcgtgcttgc
     8821 gcgacagctg cgccatcagt ggcgacaaaa agaaggtgaa cagcggcacc gccagcatga
     8881 acaagagcag cgccagcgca tggttgggca ccgcaccgcc catggccggc ggcaggttgg
     8941 gcgccacgcc gagcccggcg taaaaccacg gctgctgcgc cagccagccc agcagcgcaa
     9001 acccggccag gctggccaca aacaggccgg cgatgcgctt gacgatgtgc ttgtgcttga
     9061 agtggcccaa ttcgtgcgcc agcacggcct cgacctcggg cggggtcaac tgctcaagca
     9121 gggtgtcgaa gaacaccacg cgcttggcgg cgccaaagcc ggtgaagtag gcattggcgt
     9181 gcgcgctgcg gcggctgccg tccatcacga acaggccctt ggcggcaaag ccgcagcgcg
     9241 ccatcagggc gctgacgcgc gctttcagcg attcgtcctg cagcggctga aacttgttga
     9301 acagcggcgc gatgaccgtg gggtagatca ccagcatcaa caggctgaag gccgcccacg
     9361 cgccccacgt ccacagccac caccaggcgc cggtggcctg catcagccac aacaccagcg
     9421 ccgccagcgg cacgccgatg gcggtgccga ccagcgtgga cttgatcaga tcgcccagcc
     9481 acaggcgcag ggtcattttg ttgaagccaa aacgctcttc gatgacgaag gtctggtaga
     9541 gcgaaaacgg caggtcgatc agcgcgttga tgacggcaaa cgccaccaat agcgccagcg
     9601 gctgcagcag cggccgcggg cccaccgcat ccatcaaggc gttgttgagc caatccagcc
     9661 cgcccagcag cgtccagccc agcagcacgg cgctggccag cgccagctcc agcaggccga
     9721 agcgcagctt ggccacggtg tagtcggccg cgcgctggtg cgcatccagc gcgatggtgc
     9781 ccacgaattc ggccggtacg gcggcccggt ggcgcgccac tgcgcggatc tggcgcgacg
     9841 ccagccagaa cttgacggcc acattggcca gcagcgccag gctgaacacc aggctggtga
     9901 caagggcagg ggagagggat tcgttcaagg cgggctcggc aggatgtcag gcggcg
//
LOCUS       DAOMVO010000060         9892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1701_length_9892_cov_14.756633, whole genome shotgun sequence.
ACCESSION   DAOMVO010000060 DAOMVO010000000
VERSION     DAOMVO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1701_length_9892_cov_14.756633"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PKV30_04685"
     CDS             <1..1156
                     /locus_tag="PKV30_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRL29549.1"
                     /translation="GLHPLRGQNNVQGASDAGLIPMMFPNYQRVDNGAAHAWFEDFWS
                     TKLDDAPGYTVVEIMHKALAPDSDPHKVRGMYIMGENPAMSDPDLNHARHALASLQHL
                     VVQDIFLTETAWLADVILPASAWPEKTGTVSNTDRMVQLGQQALQPPGEARADLWIIQ
                     QMAGRMGLDWSYENDSYSRLTDKRQSPVSSENYHGVAAVYDEMRRAMHGAIAGITWDR
                     LQRESSVTYPCLSEDDPGQPIVFTDRVPTKNGKVQLVPADIIPPDERPDADYPMVLIT
                     GRQLEHWHTGAMTRRASVLDALEPAPTVSMHGDDLARLGLQPGGCARVQSRRGEVELM
                     VRRDDGTPPGSVFIPFAYREAAANLLTNAALDPFGKIPEFKYCAVRVAPV"
     assembly_gap    1339..1438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1509..2336
                     /locus_tag="PKV30_04690"
     CDS             1509..2336
                     /locus_tag="PKV30_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29550.1"
                     /translation="MSRPAARDNFADMDITRRHTQRLRQMYRSAGWPVLDVLEAELLA
                     AGLLERERGAFGCETLRVTDAGIAAISQSAAGHRAALSKHEALVCRVADEMTRAGRIA
                     WRGLAVRVPLSQAADEKQAAALTHPAQAAIVNEAAAIEDRARARWLIACPDVFSVRHT
                     SVEAYLEPVVHEIKVSRADLLGDLKRRDKRAAYLSLGGACWYVLGQDARGRPIGSADE
                     VPPECGVMHCEDGRLVIARAAPRRPIERLPFAVWMALARATPLLPPDDGQGDLANVA"
     gene            complement(2400..3611)
                     /gene="ubiM"
                     /locus_tag="PKV30_04695"
     CDS             complement(2400..3611)
                     /gene="ubiM"
                     /locus_tag="PKV30_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HRL29551.1"
                     /translation="MHFDILISGAGPAGLCLAQALSGQGLAIGIVEQQPQAAIAAPAF
                     DGREIALTQPSADTLRRLGLWQRIQDCDAAALSPLCDAKVTNGPSPFALTIGHGLTAR
                     SELGWLVSNHLIRRAAWDAVQHSMAQHGDITLIAGDGVARVAVGADAAQVTLASGATH
                     SARLLVAADSRFSPTRRAVGIGAAMHDFGRSMLVCQMTHDVPHEHTAWEWFGHGQTLA
                     LLPMNDDPVTGAHRASVVLTLPHQQIEPLLALDEAAFNSHITRRFDARLGAMGLASTR
                     HAYPLVGVYPHRMVSERFACVGDAAVGMHPVTAHGFNLGLLSVEALSRELRSAHAAGR
                     DVGAPEPLARYQRTHRLATRPLYEATRLVATLYTAEARPARFLRDAALRVAERVTPFK
                     RAIAAQLAGGR"
     gene            3765..4286
                     /locus_tag="PKV30_04700"
     CDS             3765..4286
                     /locus_tag="PKV30_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HRL29552.1"
                     /translation="MRRVPVTGVAARWKPWHWALHDVVPQQPQHGDVPHQLYDNDDGQ
                     CWIFPGHTVELFRDDAEGYYLNATSGAPGWFVMWRMHDGADGGEPMPRPEAVSVSYHD
                     AGRWLDAQETVEQVAAPPDVIEWMSAFANAHYTPEPKRRQRPQSFQPLTDRFGNPARV
                     STEEKRRGGGGGG"
     gene            4290..4934
                     /locus_tag="PKV30_04705"
     CDS             4290..4934
                     /locus_tag="PKV30_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HRL29553.1"
                     /translation="MSDEQGGGFLDRWSRRKVQARAGQTPADEAPPPVAAAVAAPVDG
                     SKTLPALADKAPPAIKEEAPVLTLEDAQALTPESDFTPFVQRAVAPEVRNAAFKKLFA
                     DPHFNVMDGLDIYIDDYSQPDPLPASMLRQMASAKFLQLVEEEPEAPPTEETAGAAPE
                     PLPPQGEDAVAQSGLCTDVPMSPPEEVAAHAQDPDLRLQPDDAAGCAPDRPGAG"
     gene            4894..6993
                     /locus_tag="PKV30_04710"
     CDS             4894..6993
                     /locus_tag="PKV30_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRL29554.1"
                     /translation="MPLDARRIGQALGEELTLHSTLCRREAPAFQRALKGDGEAVVVA
                     CTQEQRLFGELAEQTEGVTTHELKFVNIRETAGWSRDATQATPKIAALLAAARLSDPE
                     PVPVVNFKSAGRLLIIGPIDAAERAAALLQDVLDVSIFTTGAGRAGGAQARRWPVLGG
                     RLQGLKGWLGAFDVEWSASNPIDLDLCTRCNACVAVCPENAIGLDYQIDLALCQSHRA
                     CVAACSAAGAIDFERAAPLQSERFDLILDLRAADATPTFLQHAPPQGYVRWDGRDAAT
                     LLKLRDLVGEFEKPKFFQYKAAICAHARNEQVGCSACIDICSAEAISSEKSRQQIKVE
                     PHLCVGCGACTTVCPTGAISYSYPRAGEQGARIKALLSTYAAAGGQGATLMVHSQDKG
                     QALIGELGRAAQLKLMNGVPHQVMTLAVWHTASLGIDVWLSAIAWGAEQVLVLTTDEE
                     APQYLDGLQAQMDVAQALLNGLGYSGTHFKLLRARSTQDLDHALQNLQQTTQRVPKIA
                     ARFAIAPEKRSTLELALDHLIAKSPLKARPELIELPAASPFGAVLVDKDACTLCLSCV
                     NACPAGALLDHHERPELRFIEKNCVQCGLCESTCPEDAITLQPRLSLAAERAQPRVLN
                     SAQPYGCIRCGKPFGTVQAIEAMLGRLAGHAMFQGAALERLKMCSDCRVIDVYSATDE
                     VQIDAVADGRNIQGWRP"
     gene            6990..7607
                     /locus_tag="PKV30_04715"
     CDS             6990..7607
                     /locus_tag="PKV30_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HRL29555.1"
                     /translation="MSELQLSNDGLDEEIARAEVYGLLAQLYQAPPAPELLANLRVAA
                     TEAPVAGAFLEEPFRQLVGAARACSDDAVADEFEALFGGVGKPEVWAYGSHYLSGFLN
                     DKPLVRLRSDLAALGLGRDESRAETEDHIAYLCEVMRYLIAGDDVAVANLTQQQRFFA
                     AHLQPWVERLCEAIAAQPRAEFYRAVALFTQAFMNVEAQGFDLVE"
     gene            7852..8067
                     /locus_tag="PKV30_04720"
     CDS             7852..8067
                     /locus_tag="PKV30_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HRL29556.1"
                     /translation="MNRPAAHNKLSRRVLFAGAGKAGAVAALATVLPAARQPQAEVTL
                     APREAPEGGGGYALTEHVKRYYKTAGV"
     gene            8113..>9892
                     /locus_tag="PKV30_04725"
     CDS             8113..>9892
                     /locus_tag="PKV30_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRL29557.1"
                     /translation="MLLTKKPGAGAGAPVSSSFVQRLRHGLSQAVPTMDRRAFLRRSG
                     LGVGAGLAVSQLTLVKKSTAAEGAKSIDGSGAVVVRRTVCTHCSVGCASDAVVENGVW
                     VRQEPVFDSPINLGAHCAKGASLREHGHGEYRLRYPMKLVNGKYQRISWDEALNEISA
                     KMLELRKQSGPDSLYVIGSSKHSNEQAYLLRKWMSFWGSNNCDHQARICHSTTVAGVA
                     NTWGYGAMTNSYNDMQNSKCAFYIGSNAAEAHPVSMLHMLHAKETGTKMIVADPRFTR
                     TAAKADQYIRFRSGTDIPVLFGILYHIFKNGWEDKKYINDRVFGMEKVREEVLAKWTP
                     DKVEEASGAKEADLYKAAEMMAKNRPSTIVWCMGQTQHTIGNAMVRASCILQLALGNI
                     GVSGGGANIFRGHDNVQGATDVGPNPDSLPGYYGLAEGSWKHFASTWGVDYEWIKKQY
                     ASPAMMTKSGITVSRWIDGVLEKNDLIDQDPNLRAVIYWGHAPNSQTRGLEMKRAMDK
                     LDLLVVIDPYPSATAAMAAMPGQAGDLNPNRAVYLLPACTQFETSGSATASNRSLQWR
                     EKCIEPLWESRSDHMIMYQLAQKLGFD"
BASE COUNT         1662 a         3324 c         3353 g         1453 t
ORIGIN      
        1 cggcctgcac ccgctgcgcg gccagaacaa cgtgcagggc gccagcgatg cggggctgat
       61 ccccatgatg ttccccaact accagcgcgt ggacaacggc gccgcgcacg cgtggtttga
      121 agatttctgg agcaccaagc tggacgatgc gcccggctat acggtggtcg agatcatgca
      181 caaggcgctg gcgcccgaca gcgacccgca caaggtgcgc ggcatgtaca tcatgggcga
      241 gaacccggcc atgagcgacc ccgacctgaa ccacgcgcgc catgcgctgg cgtcgctgca
      301 gcatcttgtg gtgcaggaca tcttcctcac cgaaacggcg tggctggctg atgtgatttt
      361 gcccgcgtcg gcctggcccg agaagaccgg taccgtcagc aacaccgacc gcatggtgca
      421 actgggccag caggcgctgc agccgcccgg cgaggcgcgg gccgatctgt ggatcatcca
      481 gcagatggct gggcgaatgg ggttggattg gagctatgaa aacgatagct attcgcgctt
      541 gacagacaag cgccagagcc cggtttcatc tgaaaactac cacggcgtcg ccgccgtcta
      601 cgacgaaatg cggcgcgcca tgcacggcgc catcgccggc atcacctggg atcggctgca
      661 gcgcgaaagc agcgtcacct acccgtgcct gagcgaagac gacccgggcc aacccatcgt
      721 cttcaccgac cgcgtgccga ccaaaaacgg caaggtgcaa ctggtgccgg ccgacatcat
      781 tccgcccgac gagcggcccg atgccgacta cccgatggtg ctgatcaccg gccgccagct
      841 cgagcactgg cacaccggcg ccatgacgcg ccgcgccagc gtgctcgacg cgctggagcc
      901 cgctcccacc gtcagcatgc acggcgacga cctggcccgg ctcggtcttc aacccggcgg
      961 ctgcgcacgc gtgcaatcac gccggggcga ggtcgaactg atggtgcgcc gcgacgacgg
     1021 cacgccgccc ggcagcgtgt tcattccgtt tgcctaccgc gaggcggcgg ccaacctgct
     1081 gaccaacgcc gcgctcgatc cgttcggcaa gatcccggag ttcaagtact gcgcggtgcg
     1141 cgtggcgccg gtctgaagcg atcagcggcc gaacgcgtcg gcctctgtga cgtgcgcggt
     1201 gccgctcttg cgttcgaaca ggcacagttc gcggccgagc ccgttcttca tgtgtttttg
     1261 cgggtagcgg ccctgcagca gcagcgcggt gtagcccaca tcgtcgctga acagcgcagg
     1321 cttgcccagc gtcttggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     1441 tcaccccgac agcgccgccg tgtcagccaa agccccggct tgcccggcgc gatatcgcac
     1501 gcggtggcat gagccgcccc gctgcgcgcg acaatttcgc cgacatggac atcacccgcc
     1561 gccacaccca gcgcctgcgc cagatgtacc gctcggccgg ctggccggtg cttgacgtgc
     1621 tggaagccga attgctcgcc gccggtctgc tcgagcgcga gcgcggcgcc ttcggctgcg
     1681 agacgctgcg cgtcaccgat gccggcatcg ccgccatcag ccagagtgcg gccggccacc
     1741 gcgccgcgct ctcgaagcac gaagcgctgg tgtgccgcgt ggccgacgag atgacgcgcg
     1801 ccggccgcat cgcctggcgc gggctggcgg tgcgcgtgcc gctgtcgcag gcggctgatg
     1861 aaaaacaggc tgcagcgctt acccatccag cgcaagcagc tatcgttaat gaagcagccg
     1921 cgatcgaaga ccgtgcccgc gcgcgctggc tgatcgcctg ccccgatgtc ttctcggtgc
     1981 gccacaccag cgtcgaggcc tacctggaac ccgtggtgca cgagatcaag gtcagccgcg
     2041 ccgatttgct gggcgacttg aagcgccgcg acaagcgcgc ggcctacctg tcgctggggg
     2101 gagcctgctg gtacgtactg ggccaggacg cacgcggccg cccgattggc agcgccgacg
     2161 aggtgccgcc cgaatgcggc gtgatgcatt gcgaggacgg ccgcctggtg atcgcgcgcg
     2221 ccgccccgcg ccggccgatc gagcgcctgc ccttcgccgt ctggatggcg ctggcgcgcg
     2281 ccacgccgct gctgccgccc gacgatgggc agggcgatct ggcgaacgtg gcttgaagtt
     2341 tcaggccttt gatgccgcca gcgcaggcgc agcaagcgcc agcagctatc aaaaacgtat
     2401 caacgaccgc ccgccagttg cgccgcgatg gcgcgcttga acggcgtcac gcgctcggcc
     2461 acgcgcagcg cggcgtcgcg caaaaagcgc gccggccttg cctcggcggt gtacagcgtg
     2521 gccaccaggc gtgtggcttc gtacagcggg cgggtggcca ggcggtgcgt gcgctggtag
     2581 cgcgccagcg gctcgggtgc gcctacgtcg cgccccgcgg cgtgcgcact gcgcagctcg
     2641 cgcgaaagcg cctcgacact cagcagcccc aagttgaagc catgcgccgt caccgggtgc
     2701 atgcccaccg ccgcgtcgcc cacgcaggca aagcgttcgc tcaccatgcg gtgcgggtac
     2761 acgcccacca gcgggtaggc gtggcgcgtg ctggccaggc ccatggcgcc caggcgggcg
     2821 tcgaagcggc gcgtgatgtg gctgttgaag gcggcttcat ccagcgccag cagcggttcg
     2881 atctgctggt gcggcagtgt cagcaccacc gaggcgcggt gcgcgcccgt cacgggatcg
     2941 tcgttcatcg gcagcagcgc cagcgtctgg ccgtggccaa accattccca cgcggtgtgt
     3001 tcgtgcggca cgtcgtgcgt catctggcac accagcatgc tgcggccaaa gtcgtgcatc
     3061 gccgcgccaa tgccgacggc gcgccgcgtg ggcgaaaagc ggctgtcggc cgccaccagc
     3121 agccgcgccg aatgcgtggc gccgctggcc agcgtgactt gcgccgcatc ggcgcccacc
     3181 gccacgcgcg ccacgccgtc gccggcgatc agcgtgatgt cgccgtgctg cgccatgctg
     3241 tgctgcaccg cgtcccacgc cgcgcggcga atcaggtggt tggagaccag ccagcccagc
     3301 tcgctgcgcg ccgtcaggcc gtggccaatc gttagcgcga agggcgaggg gccgttcgtc
     3361 accttggcat cgcagagtgg cgacagcgcc gccgcgtcgc agtcctggat gcgctgccac
     3421 aggccgagtc gccgcaacgt gtcggccgaa ggctgcgtga gcgcgatctc gcgcccgtcg
     3481 aacgccggcg cggcgatggc cgcctgcggc tgctgttcca cgatgccgat ggccaagccc
     3541 tggcctgaca gcgcttgcgc caggcacagg ccggcggggc cggcgccgct gatgagaatg
     3601 tcgaagtgca tggccgggca gggtagcggc ctgcgcgcgg cagggttttg atgcctgtca
     3661 agctgcgggc tggggcaaat aagcgtgcgc cggataatgg ggcggtgacc gcgccagatt
     3721 cttcgcccct ttccgcccgc cccacgctgc atgtggccgt gctgatgcgc cgcgtgccgg
     3781 tgaccggcgt ggccgcgcgc tggaagccct ggcactgggc gctgcacgac gtggtgccgc
     3841 agcagccgca gcacggcgac gtgccgcacc agctgtacga caacgacgac gggcagtgct
     3901 ggatttttcc cggtcacacg gtcgagctgt ttcgcgacga tgccgagggt tactacctca
     3961 acgcgaccag cggcgcgccg ggctggttcg tgatgtggcg catgcacgat ggtgccgacg
     4021 gcggtgagcc gatgccgcgg cccgaggcgg tgtcggtgag ctaccacgac gccggccgct
     4081 ggctcgatgc gcaggagacg gtggagcagg tggccgcgcc gcccgacgtg atcgagtgga
     4141 tgagcgcgtt tgccaacgcg cactacacgc ccgagcccaa gcgccgccag cggccgcaga
     4201 gctttcagcc gctgaccgac cgcttcggca acccggcgcg cgtgtcgacc gaggagaagc
     4261 ggcgtggcgg tggcggcgga ggttgaacga tgagcgacga acagggcggt ggttttctgg
     4321 accgctggtc gcgccgcaag gtgcaggcgc gcgcggggca gacgccggcc gatgaagcgc
     4381 cgccgcccgt ggcggccgcc gttgcagcgc cggttgatgg atcaaaaacg ctgccagcgc
     4441 ttgccgataa agcgccacca gctatcaaag aagaagcgcc ggtgctgacg ctggaggatg
     4501 cgcaggccct cacgcccgag tccgacttca cgcccttcgt gcagcgcgcg gtggcgcccg
     4561 aagtgcgcaa cgcggcgttc aagaagcttt ttgccgaccc gcacttcaac gtgatggacg
     4621 ggctcgacat ctacatcgac gattactcgc agcccgatcc gctgcccgcc agcatgctgc
     4681 ggcagatggc gagcgcgaag ttcctgcaac tggtcgagga agagcccgaa gcgccgccga
     4741 ccgaggaaac agccggcgcg gcgcctgaac ccctgccgcc gcaaggggaa gacgctgtgg
     4801 cacagtccgg gttatgtacc gatgtgccta tgtcgccgcc cgaggaggtg gccgcccatg
     4861 cccaagaccc tgatctgcga ctgcaaccag acgatgccgc tggatgcgcg ccggatcggc
     4921 caggcgctgg gtgaagagct gaccctgcat tccacgctgt gccgccgcga ggcgccggcg
     4981 tttcagcgcg cgctcaaggg cgatggcgaa gccgtggtgg tggcttgcac gcaagagcag
     5041 cgcctgtttg gcgaactggc cgagcagacc gagggcgtga ccacgcacga gctgaagttc
     5101 gtcaacatcc gcgagaccgc cggctggagc cgcgacgcga cacaagccac gcccaagatc
     5161 gccgccctgc tggccgccgc gcgcctgtcc gaccccgaac cggtgccggt ggtcaacttc
     5221 aaaagcgccg gtcgactgct gatcatcggc cccatcgatg ccgccgagcg cgccgccgcg
     5281 ctgctgcaag acgtgctgga cgtgagcatc ttcaccaccg gcgccggccg cgcgggcggc
     5341 gcccaggcgc ggcgctggcc ggtgctgggc gggcggctgc aagggcttaa gggctggctg
     5401 ggtgccttcg acgtcgaatg gtcggccagc aacccgatcg acctcgacct gtgcacgcgc
     5461 tgcaacgcct gcgtggccgt gtgccccgaa aacgcgatcg ggctcgacta ccagatcgac
     5521 ctggcgcttt gccagtcgca ccgggcctgc gtcgccgcct gcagtgcggc aggcgctatc
     5581 gattttgagc gcgccgcgcc gctgcagagc gagcgcttcg acctcatcct tgatctgcgc
     5641 gcggccgacg ccacgcccac ctttttgcag cacgcgccgc cgcaaggcta cgtgcgctgg
     5701 gacgggcgcg acgccgccac gctgctcaag ctgcgcgatc tggtcggcga gttcgagaag
     5761 cccaagttct ttcaatacaa ggccgccatt tgcgcccatg cgcgcaacga gcaggtcggt
     5821 tgcagcgcct gcatcgacat ctgctcggcc gaggcgatca gcagcgaaaa atcacggcag
     5881 cagatcaagg tcgagcccca cctgtgcgtc ggctgcggcg cctgcaccac ggtgtgcccg
     5941 accggcgcca tcagctactc ctacccgcgc gccggcgaac agggcgcgcg catcaaggcg
     6001 ctgctcagca cctacgccgc cgcaggcggg cagggcgcga cgttgatggt gcacagccaa
     6061 gacaaagggc aggcgctgat cggtgaactc ggccgcgccg cgcaactgaa gctgatgaac
     6121 ggcgtgccgc accaagtgat gacgctggcc gtgtggcata cggcgagtct gggcatcgac
     6181 gtctggctgt cggccatcgc ctggggcgcc gagcaggtcc tggtgctgac caccgatgag
     6241 gaagcgccgc agtacctgga cggcctgcaa gcgcagatgg atgtcgcgca ggcgctgctg
     6301 aacggcctcg gctattcggg cacgcatttc aagctgttgc gcgcgcgctc gacgcaggat
     6361 ctggatcatg cgctgcagaa tctgcagcag accacgcagc gcgtgcccaa gatagcggcg
     6421 cgctttgcca tcgcgcccga aaagcgcagc acgctggagc tggcgctgga tcacctgatc
     6481 gccaagtcac cgctgaaggc gcggcccgag ctgatcgagc tgccggcggc atcgcccttt
     6541 ggcgccgtgc tggtcgacaa ggacgcctgc acgctgtgcc tgtcgtgcgt caacgcctgc
     6601 ccggcgggcg cgctgctcga ccaccacgag cggccagagc tgcgcttcat cgagaagaac
     6661 tgcgtgcagt gcggcctgtg tgaaagcacc tgccccgaag acgccatcac cctgcagccg
     6721 cgcctgtcgc tggcggccga gcgcgcgcag ccgcgcgtgc tcaacagcgc ccagccctac
     6781 ggctgcatcc gctgcggcaa gccctttggc acggtgcagg ccatcgaggc catgctgggc
     6841 cggctggcgg gccacgccat gtttcagggc gcggcgctgg agcggctgaa gatgtgctcc
     6901 gactgccgcg tgatcgacgt ctattcggcc accgacgagg tgcagatcga cgccgtggcc
     6961 gatggccgca acatccaggg ctggcggcca tgagcgaatt gcagttgtcg aacgacgggc
     7021 ttgacgagga aatcgcccgt gcagaggttt atgggctgct ggcgcagctg tatcaagcgc
     7081 cgccagctcc tgaattgcta gcgaacctgc gcgtggcggc gaccgaggcg ccggtggccg
     7141 gcgcgtttct tgaagagccg tttcgccaac tggtgggcgc cgcgcgggcc tgcagcgacg
     7201 acgcggtggc ggacgagttc gaggcgctgt tcggcggcgt cggcaagccg gaggtgtggg
     7261 cctacggctc gcactacctg agcggctttc tcaacgacaa gccgctggtg cgcctgcgca
     7321 gcgatctggc cgcgctcggc ctgggtcgcg acgaatcgcg cgccgagacc gaagaccaca
     7381 ttgcctacct gtgcgaggtg atgcgctacc tgattgccgg cgacgacgtg gccgtggcca
     7441 acctgacgca gcagcagcgc ttttttgctg cgcatcttca gccgtgggtc gagcgcctgt
     7501 gcgaggccat cgcggcgcag cccagggctg agttctaccg ggcggtggcc ctgttcaccc
     7561 aggccttcat gaacgtcgag gcgcagggtt tcgatctggt ggagtgacgc ccggcattgc
     7621 ggcacttctg ccgcgcgctg catcagagcc attccggcct ccagcgccgg ccatgcttgc
     7681 gtgggcagct acaaaaaggt aaggaccaag gcaggccgaa atggcatgaa acgcgcggga
     7741 ttaccagcgg taatgacggg ggtcggtcgt atatcatgcg atgacaaccc atgatttgat
     7801 ttgcataggt tgggcttggc aaaacggcaa gacccagcca ggagagccgc gatgaaccga
     7861 cctgctgccc acaacaaact ctcgcgccgc gtgctgtttg ccggtgctgg caaagcgggg
     7921 gcggtggccg ccctggctac cgtgctgccc gccgcgcgcc agccgcaagc cgaggtgacc
     7981 ctggccccgc gcgaagcgcc cgaaggcggc ggcggctacg cgctgaccga gcacgtcaag
     8041 cgctactaca aaaccgccgg cgtttgatgc cccgcggccg tccccacact ttgattgcgt
     8101 gaggcctcac tcatgttgct gaccaagaaa cccggcgccg gtgcgggcgc acccgtgtct
     8161 tcctccttcg tgcagcgctt gcggcacggc ttgtcgcagg cggtgccgac gatggaccgg
     8221 cgagcgtttt tgcgccgctc gggcctgggc gtcggtgctg gcctggcggt gtcgcagctg
     8281 acgctggtca agaagtccac cgcggccgaa ggcgccaaga gcattgacgg cagcggcgcc
     8341 gtggtggtgc ggcgcacggt gtgcacgcac tgctcggtcg gttgcgcgtc ggatgcggtg
     8401 gtcgagaacg gcgtctgggt gcggcaagag ccggtgttcg attcgcccat caacttgggc
     8461 gcgcactgcg ccaagggcgc ttcgctgcgc gaacacggcc acggcgagta ccgcctgcgc
     8521 tacccgatga agctggtgaa cggcaagtac cagcgcatca gctgggacga ggcgctgaac
     8581 gagatcagcg ccaagatgct ggagctgcgc aagcaaagcg gcccggacag cctgtacgtc
     8641 atcggctcct ccaagcacag caacgaacag gcttacctgc tgcgcaagtg gatgagcttc
     8701 tggggcagca acaactgcga ccaccaggcg cgcatttgcc acagcaccac cgtggcgggt
     8761 gtggccaaca cgtggggcta cggcgccatg accaactcgt acaacgacat gcagaacagc
     8821 aagtgcgcgt tctacatcgg ctccaacgcc gccgaagcgc acccggtcag catgctgcac
     8881 atgctgcacg ccaaggaaac cggcaccaag atgatcgtgg ccgacccgcg cttcacgcgc
     8941 acggcggcca aggcagacca gtacatccgc ttccgctcgg gcaccgacat cccggtgctg
     9001 ttcggcatcc tgtaccacat cttcaagaac ggctgggaag acaagaagta catcaacgac
     9061 cgcgtcttcg gcatggaaaa agtgcgcgag gaagtgctgg ccaagtggac gcccgacaag
     9121 gtcgaggaag ccagcggcgc gaaagaggcc gacctgtaca aggcggccga gatgatggcc
     9181 aagaaccgcc ccagcaccat cgtctggtgc atgggccaga cgcagcacac catcggcaac
     9241 gccatggtgc gcgcctcgtg catcctgcag ctggcgctgg gcaacattgg tgtgtcgggc
     9301 ggcggcgcca acatcttccg cggccacgac aacgtgcagg gcgccaccga cgtcggcccc
     9361 aaccccgatt cgctgccagg ctactacggc ctggccgaag gttcgtggaa gcacttcgcc
     9421 agtacctggg gcgtggacta cgagtggatc aagaagcagt acgccagccc ggccatgatg
     9481 accaagagtg gcatcacggt gagccgctgg atcgacggcg tgctggagaa aaacgacctg
     9541 atcgaccagg acccgaacct gcgcgccgtc atctattggg gtcacgcgcc caactcgcag
     9601 acgcgcgggc tggagatgaa gcgggccatg gacaagctgg acctgctggt ggtgatcgat
     9661 ccctacccat cggccaccgc ggccatggcc gccatgccgg gccaggcggg cgacctcaac
     9721 cccaatcgcg ccgtgtacct gctgcccgcg tgcacgcagt tcgagaccag cggctcggcc
     9781 acggcgtcca accgttcgct ccagtggcgc gagaaatgca tcgaaccgct gtgggagagc
     9841 cgcagcgacc acatgatcat gtaccagctg gcgcagaagc tgggctttga ca
//
LOCUS       DAOMVO010000061         9885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1705_length_9885_cov_20.726450, whole genome shotgun sequence.
ACCESSION   DAOMVO010000061 DAOMVO010000000
VERSION     DAOMVO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_9885_cov_20.726450"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKV30_04730"
     CDS             <1..504
                     /locus_tag="PKV30_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL29558.1"
                     /translation="THPALRQLFAQAAQGLHSGAGWFNRARDFPNTRTSELPVSPEGD
                     RAINGTPPFWQRYLPFWASNLLERMWLVIGGLIVLLLPLSRVVPPLYTYRVRQRIFRW
                     YARLRGVEAKVEEGLGERDELLNELDELDRVTNGITVPLAHAYELFALRNNIDAVRRR
                     LLAKRPG"
     gene            complement(517..1125)
                     /gene="ttdB"
                     /locus_tag="PKV30_04735"
     CDS             complement(517..1125)
                     /gene="ttdB"
                     /locus_tag="PKV30_04735"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HRL29559.1"
                     /translation="MKKILTTPIKDEDLESLNAGDVVYLTGTLITCRDVAHRRLIEQG
                     RDLPVDLKGGAIFHAGPIVRQKDDGEYEMVSIGPTTSMRMEKFEKDFIKQTGVKLIVG
                     KGGMGAETQEGCVENKAVHAIFPGGCAVLAATKVEKIEGAEWKDLGMPETLWINKVKE
                     FGPLIISIDTKGRNLIEENKKVFNEKKKPILERINQQIGFIK"
     gene            complement(1122..2021)
                     /gene="ttdA"
                     /locus_tag="PKV30_04740"
     CDS             complement(1122..2021)
                     /gene="ttdA"
                     /locus_tag="PKV30_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002163154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HRL29560.1"
                     /translation="MNREQAVADLTNVMSKFTAYIGKRLPTDVTQKLGDLREKEVNFL
                     AKEVYESMRQNQEAADKLDRPSCQDTGVIQYFLTAGANFPLLGELEEILQEATAGATR
                     EGPLRHNAVETFVEKNTGTNTGSKIPWLDWDIVPGDDSVTIDVYMAGGGCTLPGKATV
                     LMPGQGYEGVAEFVFDVITSRGVNACPPLLVGVGVSTSAETAARLSKKAILRPVDSRH
                     PNDNAALMEQLLTDGLNELGLGPQGLTGNNSVMGVNIESSARHPSTIGVAVSTGCWAH
                     RRGRIRINADMSYEVISHQGFKL"
     gene            complement(2041..3033)
                     /locus_tag="PKV30_04745"
     CDS             complement(2041..3033)
                     /locus_tag="PKV30_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29561.1"
                     /translation="MPHAERFPFTRRTALQLAALALASVAGAAHAQAWPSKPVSLVVP
                     FPAGGTTDVLARALAERLSPAIGQPVIVENKPGAGATIGADYVAKAKADGHTLLIGAV
                     HHTIASSVYKKLPYDFQKGFEPVTVIAMVPNVLVVNARTPAKNVNELVALIKAKPAEA
                     SYGSNGNGTAQHLIGTQFQQQTGTRLQHIPYKGSGPLATDLLGGQILMSFDTITPVLP
                     HIKAGKLRPLAVTTAKRSPALPDVPTLQEAGLKDFDIGTWFGVLAPVGTPKPVLDRLS
                     AEATKIIQSPDFRKRMDDIGAQPVGNSPAEMAAQIRSETDKFSVLVKAGNVTVE"
     gene            3168..4088
                     /locus_tag="PKV30_04750"
     CDS             3168..4088
                     /locus_tag="PKV30_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL29562.1"
                     /translation="MNPAIQPSDLGFFSALARAGSLSAAARDLRVTTAAVSRHLAQME
                     KRLGVLLVNRTTRRMGLTPEGEVYLEHARRILGDIDNLGELLGGAQSSPQGLLRVNAT
                     LGFGRSHIAPLISRFARRFPQVEVQLQLSVNPPPITDDAFDVCIRFGAPPDARVIARY
                     VAPNRRLLCAAPAYLKRHGAPKTPGDLARHACIGIRQGEEAYGLWRLSPAKGRPGRAE
                     SVKTRGPLSTNDGEIAVKWALDGHGILLRAEWDVAPYLQSGRLVQVLPQYVTPDADIY
                     AVYPQRHQLAARVRVFVDFVTQGFDKLGAA"
     gene            complement(4098..5114)
                     /locus_tag="PKV30_04755"
     CDS             complement(4098..5114)
                     /locus_tag="PKV30_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRL29563.1"
                     /translation="MTTRSTSGRPRIAVVGTGGTIAGAGNAADGAPSAAYTSAVTGAD
                     ALIAGVPALAQLADVQAEQLLQIDSADFTDAQLLLLARRVVALCVRDDVDGVVITHGT
                     DTMEESAYFLHLTAHSAKPIVLTGAMRPGTALAADGPANLLHAVAVAAHPSSAGRGVL
                     VVMNEEVHSARDVAKVHAMRLDAFASPHGPLGLVVEGVPRWYRAVTRPHTTTSEFDIA
                     AIDALPLVGVVTSHGNMRREIYDAWLAAGARAIVHAGFGGGTVPEYLKPVLAELCAAG
                     VPIVRCSRVGAGPVIRNASFDDDACGSVAADDQNLPRARLLAALALTRSQGVGEMQRI
                     FNRY"
     gene            5221..5562
                     /locus_tag="PKV30_04760"
     CDS             5221..5562
                     /locus_tag="PKV30_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29564.1"
                     /translation="MKAKAPSVLLLAGLLFGGAALAMPSAENSDAVLPNSPLKERGYP
                     DAGRFNPVGGDVAEMGVAPAVYLAPAAITYPPAIVIDVRPPKPYALVAAPTLPCAPGY
                     CPRLRPRPDRN"
     gene            5807..7714
                     /locus_tag="PKV30_04765"
     CDS             5807..7714
                     /locus_tag="PKV30_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRL29565.1"
                     /translation="MSQLDHNPDATPQNPAARVQTGTAAPARDRRRPSFDFFAPEVWQ
                     RFWAIASPYWREGEKGKAWGFLILLIAMLLAETQIAVMLNDQAGEMTSALAERNGERF
                     WHAIMVCLIVVAVFVPTRSLYYFVRDTLGNHWRRWLSERLLGRYFLNQRFYELTAESS
                     IDNPDQRIAEDVNTFTQRSLYFLLIGLGSLMQLVAFSWVLWSISHTLVYVLVAYTALG
                     TWLTVKVFGAPLMRLNFFQLKREADFRFSLVRVRENAESIAFYRGERAELKQVRGRFK
                     GALRNYQRLLRQQLNLNFFQQAYNQIAIILPNILIANLVLSGQLEVGQAIKAAGAFLA
                     VLGAVSIIIDNFEGLSRFTAGIERIHGLLAALPAQPGESDEAVAAEPPPLARRRRHTI
                     ETTEAPELSLQGVTLNTPDGKRELVRDLSFTLHKGDGLLIVGESGSGKSSLLRAIAGL
                     WRSGEGSIQRPPVADSMFLPQRPYMQVGSLRSQLLYPQFDRELDDAVLHQALHDVNLP
                     DLVQRSGGLDTEVDWAKTLSLGEQQRLAFARVLISAPRFVILDEATSALDAANEARLY
                     RRLLAEDTTLISIAHRAAVLDHHTHVLELTGGGRWALHQAEGFAFAEGPSSRSSAADT
                     AATGDGAAPAN"
     gene            7802..8335
                     /locus_tag="PKV30_04770"
     CDS             7802..8335
                     /locus_tag="PKV30_04770"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRL29566.1"
                     /translation="MKPLSAATLLLASLLSTPITALAALKAGDAAPPFKTPAAVAGQA
                     FDFDLSAALKKGPVVLYFFPKAFTKGCTVEANAFAEATPQFAALGATVVGISHDDIDT
                     LKKFSTEECRNKFAVASDPKALTIKAYDAGSLLPGMASRISYVIGPDGKIAFTHEGSN
                     PIKHVEQTLEAVRRLKK"
     gene            complement(8444..8977)
                     /locus_tag="PKV30_04775"
     CDS             complement(8444..8977)
                     /locus_tag="PKV30_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRL29567.1"
                     /translation="MFDMSVPWWEFVVRGVVVYLFLLFFLRISGRRQIGQYDPFDLIL
                     LLILSNAVQNSMNAGDNSLIGGLISALTLIGCHVVMAHLMWRSKRLARLIDGKPQVLI
                     RTGALDENLMRAERLTADDVHAALRAAGCLHTHEVERATIETNGQITVVLRNRDSWRG
                     MPPSAPGAPSERGDDVT"
     gene            9165..9524
                     /locus_tag="PKV30_04780"
     CDS             9165..9524
                     /locus_tag="PKV30_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29568.1"
                     /translation="MPIRTRHFSPALVIAAALLAACGQKDAGTPQIPAPAGEAMTKAI
                     EAQGAANQAAAATASAASAAADASQSAAAAGTSTSDAQAAAAAAVAAATMAASAATEA
                     KQATADSAAAPASAASQ"
     gene            complement(9550..>9885)
                     /locus_tag="PKV30_04785"
     CDS             complement(9550..>9885)
                     /locus_tag="PKV30_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL29569.1"
                     /translation="HAIPQARQVHEQFADQGVAMIGLHTVFEHHDAMQPHALAAFIHE
                     YGLRFPVGVDAPSADVAPFPRTMARWGLQGTPTTLVVDRQGRLRLRHFGHVPDLPLGA
                     ILGRLLSEA"
BASE COUNT         1570 a         3428 c         3248 g         1639 t
ORIGIN      
        1 acgcaccccg cgctgcgcca gttgttcgcg caggcggcgc aggggctgca cagcggcgcc
       61 ggctggttca accgggcgcg cgactttcca aacacgcgca ccagtgaact gccagtgagc
      121 ccagagggcg accgcgccat caatggcacg ccgccgttct ggcagcgcta cttgccgttc
      181 tgggccagca acttgctgga gcgcatgtgg ctggtgatcg gcggcctgat cgtgcttctg
      241 ctgcccttga gccgcgtggt gccaccgctg tacacctacc gcgtgcgcca gcgcatcttc
      301 cgctggtacg cacggctgcg cggcgtcgag gcgaaggtcg aagagggcct gggcgagcgc
      361 gacgaactgc tcaatgagct ggacgaactc gaccgcgtga ccaacggcat caccgtgccg
      421 ctggcgcacg cctacgagct gtttgcgctg cgcaacaaca tcgacgccgt gcggcggcgg
      481 ctgctggcca agcggccggg gtgaggccgc cgcgccctat ttgatgaagc cgatctgctg
      541 attgatccgc tccaggatcg gtttcttctt ttcgttgaag accttcttgt tctcctcgat
      601 caggttgcgc cccttggtgt cgatggagat gatgagcggg ccgaactcct tgaccttgtt
      661 gatccacagc gtctcgggca tgcccaggtc cttccactcg gcgccttcga tcttttcgac
      721 cttggtcgcc gccagcacgg cgcagccgcc ggggaagatg gcgtgcaccg ccttgttttc
      781 gacgcagcct tcctgcgtct cggcgcccat gccgcccttg cccacgatca gcttcacgcc
      841 ggtctgcttg atgaagtcct tctcgaactt ctccatgcgc atgctggtgg tcggaccgat
      901 ggaaaccatc tcgtactcgc catcgtcctt ctgccgcacg atggggccgg cgtgaaagat
      961 ggcgccgcct ttcaggtcca ccggcagatc gcggccctgc tcgatcaggc ggcggtgcgc
     1021 cacgtcgcgg caagtgatca gcgtgcccgt caggtacacc acgtcgccgg cgttcagcga
     1081 ttcgaggtct tcgtccttga tcggtgtggt gaggattttc ttcacagctt gaacccctgg
     1141 tgcgagatga cttcgtagga catgtcggcg ttgatcctga tgcgcccgcg acggtgcgcc
     1201 cagcagccgg tcgacaccgc cacgccgatg gtggatgggt ggcgcgccga cgattcgatg
     1261 ttgacgccca tcacgctgtt gttgccagtc aggccctgcg gccccaggcc cagctcgttc
     1321 aggccgtcgg tcagcagttg ctccatcagc gcggcgttgt cgttggggtg ccgtgaatcc
     1381 accgggcgca ggatggcctt cttcgacagc cgcgccgccg tctcggccga ggtggacacg
     1441 cccacgccca ccaacagcgg cgggcaggcg ttgacgccgc gcgaggtgat gacgtcgaac
     1501 acgaactcgg ccacgccttc atagccctgc cccggcatca gcaccgtggc cttgccgggc
     1561 agcgtgcagc cgccgccggc catgtagaca tcaatggtca cgctgtcgtc gccgggcacg
     1621 atgtcccaat ccagccaagg gatcttcgag ccggtgttgg tgccggtgtt cttctcgacg
     1681 aaggtctcga ccgcgttgtg gcgcagcggg ccttcgcgcg tggcgccggc cgtggcttcc
     1741 tgcagtattt cttctagctc gcccagcagc ggaaagttgg cgccggcggt caggaagtac
     1801 tggatcacgc cggtgtcctg gcagctcggc cgatcaagct tgtcggccgc ttcctggttc
     1861 tggcgcatgg attcatagac ttccttggcg agaaagttga cttccttttc gcgcaggtcg
     1921 cccagttttt gtgtcacatc ggtcggcagg cgctttccga tgtaggcggt gaatttggac
     1981 atgacgttgg tcaggtcggc gacggcttgt tctctgttca tgcgttgctc caatcgcgct
     2041 tcattcgacc gtgacgttgc cggccttcac cagcacgctg aacttgtccg tctcggaccg
     2101 gatctgcgcc gccatctcgg ccgggctgtt gcccacgggc tgcgcgccga tgtcgtccat
     2161 gcgctttctg aagtcgggcg actggatgat cttggtggcc tcggccgaca ggcggtccag
     2221 caccggcttg ggcgtgccca ccggcgccag cacgccaaac caggtgccga tgtcgaagtc
     2281 cttcaggccc gcctcttgca gcgtcggcac gtcgggcagc gcgggcgagc gcttggcggt
     2341 ggtcaccgcc agcgggcgca gcttgccggc cttgatgtgc ggcagcaccg gagtgatggt
     2401 gtcgaaggac atcaggatct ggccgcccag caggtcggtg gccagcgggc cgctgccttt
     2461 gtagggaatg tgctgcagcc tggtgccagt ctgctgctga aactgcgtgc cgatcaggtg
     2521 ctgcgccgtg ccgttgccgt tggagccgta gctggcctcc gccggcttgg ccttgatcag
     2581 tgccaccagc tcgttcacgt tcttggccgg cgtgcgggcg ttgaccacca gcacgttggg
     2641 caccatggcg atcacggtca caggctcgaa gcccttttga aagtcgtagg gcagcttctt
     2701 gtacacgctg cttgcaatcg tgtggtgcac ggcgccgatc agcagcgtgt ggccgtcggc
     2761 cttggccttg gcaacgtagt cggcgccaat ggtggcgccg gcgccgggct tgttctcgac
     2821 gatgaccggc tggccgatgg cgggcgacag gcgctcggcc agcgcgcgcg ccagcacgtc
     2881 ggtggtgccg ccggccggga aaggcaccac caggctcacc ggcttgctcg gccaggcctg
     2941 ggcgtgcgcg gcaccggcga cggatgccag cgccagcgcc gccagctgca gcgcggtgcg
     3001 gcgcgtgaag gggaatcttt cggcgtgggg catgtgtctc cttgcagggt tgatcgttgg
     3061 atggatcgac tgtaggagtg catcacctgt tcagatctcg cagaaaatca aaccatcgtt
     3121 gacttctgat taacaatgag aacgtatcca ttgaaggaag cgcgcacatg aaccccgcca
     3181 ttcagccctc cgatctcggc ttcttctcgg cgttggcgcg cgccggcagc ctgagcgccg
     3241 ccgcgcgcga tctgcgcgtg accacggccg ccgtcagccg tcatctggcg cagatggaaa
     3301 agcgcctggg cgtgttgctg gtcaaccgca ccacgcgccg catgggcctg acgcccgaag
     3361 gcgaggtgta cctggagcac gcgcgccgca tcctgggcga catcgacaac ctgggcgagt
     3421 tgctgggcgg cgcgcagtcg tcaccgcagg ggttgttgcg cgtcaatgcc acgctgggct
     3481 ttggtcgcag ccacatcgcg ccgctgatct cgcgctttgc gcggcgcttt ccgcaggtcg
     3541 aggtgcagct gcagctgtcg gtcaacccgc cgccgatcac cgacgatgcg ttcgacgtct
     3601 gcatccgctt tggcgcgccg ccggatgcgc gcgtgatcgc acgctacgtg gcgcccaacc
     3661 ggcgcctgct gtgcgccgcg ccggcctacc tgaagcgcca tggcgcaccg aagacgcccg
     3721 gcgatctggc gcgccacgcc tgcatcggca ttcgccaggg cgaggaggcc tatggcctgt
     3781 ggcgcctgag ccccgccaag ggccgcccgg ggcgcgccga atcggtcaag acgcgcggac
     3841 cgctcagcac caacgacggt gaaatcgccg tcaagtgggc gctggacgga cacggcattc
     3901 tgctgcgcgc cgagtgggac gtggcgccct atctgcaaag cggccgtctg gtgcaggtgc
     3961 tgccgcagta cgtgacgcca gacgccgaca tctatgccgt ttatccgcag cgccaccagc
     4021 tggccgcgcg cgtgcgggtg ttcgtggact tcgtgacgca ggggttcgac aagctgggcg
     4081 ctgcgtgagc gcccgtctca gtagcggttg aagatgcgct gcatctcgcc cacgccttgc
     4141 gagcgcgtca gcgccagagc cgccagcagc cgcgcgcgtg gcaggttctg gtcgtcggcg
     4201 gcgacgctgc cgcaggcgtc gtcgtcgaag ctggcgttgc ggatgaccgg gccggcgccg
     4261 acgcgcgagc agcgcacgat gggcacgcca gcggcacaca gttcggccaa cacgggcttc
     4321 aggtactcgg gcaccgtgcc gccgccaaag ccggcgtgca cgatggcgcg tgcgccagcc
     4381 gccagccagg cgtcgtagat ttcgcgccgc atgttgccgt ggctggtgac cacgccgacc
     4441 agcggcagcg cgtcgatggc cgcgatgtcg aattcgctcg ttgtggtgtg cggccgcgtc
     4501 acggcccggt accagcgcgg cacaccttcg accaccagcc caagcgggcc gtgcggcgag
     4561 gcaaaggcat cgagccgcat ggcgtgcacc ttggcgacgt cgcgcgcgct gtgcacttcc
     4621 tcgttcatca ccaccagcac gccgcgcccg gccgacgaag ggtgcgccgc caccgccacg
     4681 gcgtgcagca ggttggctgg cccgtcggcc gccagcgcgg tgccggggcg catggcgccg
     4741 gtcagcacaa tgggtttggc gctgtgcgcc gtcagatgca gaaagtaggc cgactcttcc
     4801 atcgtgtcgg tgccgtgggt gatgaccacg ccatcgacgt cgtcccgcac gcacagcgcg
     4861 accacgcggc gcgcgagtag cagcagttgg gcgtcggtga aatcggccga atcgatctgc
     4921 agcagttgct cggcttgcac atcggccagc tgcgccagcg ccggcacgcc agcgatcagc
     4981 gcgtcggcgc ctgtgacggc cgaggtgtat gccgccgagg gcgcgccgtc tgctgcgttg
     5041 ccggcccctg cgatggtgcc gccggtgccg accacggcga tgcgcggccg gccgcttgtg
     5101 gaacgtgtcg tcatgcgccg cattgtcgtg taaggcgtac gtttggcggg cccaggcatc
     5161 gacaagcggc ggcaccgttc gttacactta cccgcacatc tgctgacgag agcgcaacac
     5221 atgaaagcca aagccccatc cgttctgctg ctggccggcc tgctgtttgg cggcgccgcg
     5281 ctggccatgc cgagcgccga aaactccgac gccgtgctgc ccaattcgcc gctcaaggaa
     5341 cggggctacc cggatgcggg ccgcttcaat cccgtgggtg gtgacgtggc ggagatgggc
     5401 gtggcgcctg ccgtctacct ggcgccggcc gccatcacct acccgccggc gatcgtgatc
     5461 gacgtgcgcc cacccaagcc gtatgcgctg gtggccgcgc ccacgctgcc gtgcgcgcct
     5521 ggctattgcc cccgcctgcg cccgcggccc gaccgcaact gaccctgtac gcggcgctgc
     5581 cgccgccgtt tcaagccaaa tcaggctcca gcgcacgtcc agaaagcgcg agcagctcct
     5641 gatttggaag caaagccacc gcctcaggtg gcgttttgca tggtgcgttc aaacgaattc
     5701 cgctgcccgc ctacgccccg ctcggccatt tatccgacag ccgcacggtg acggcttcgt
     5761 tacgcttggt gacccgaagt aaaccgtgcc ctcgccgccg cccaccatga gccagctgga
     5821 tcacaacccc gacgccacgc cgcagaaccc ggctgcgcgg gtgcagacgg gcaccgccgc
     5881 gccagcgcgc gaccggcggc ggccgagctt cgacttcttc gcgcccgagg tctggcagcg
     5941 cttctgggcc atcgcctcgc cctactggcg cgaaggtgaa aagggcaagg cctggggctt
     6001 cctgatcctg ctgatcgcca tgctgctggc cgagacgcag atcgccgtca tgctgaacga
     6061 ccaggccggc gagatgacct cggcgctggc cgagcgcaat ggcgagcgct tctggcacgc
     6121 catcatggtc tgcctgatcg tggtggcggt gttcgtgccg acgcgctcgc tgtactactt
     6181 tgtgcgcgac acgctgggca accactggcg gcgctggctg tccgagcggc tgctggggcg
     6241 gtacttcctc aaccagcgct tttacgagct gacggccgaa agcagcatcg acaaccccga
     6301 ccagcgcatt gccgaagacg tcaacacctt cacgcaacgc tcgctgtact tcttgctgat
     6361 cggcctcggc tcgctgatgc agctggtggc tttcagctgg gtgctgtgga gcatctcgca
     6421 cacgctggtg tacgtgctgg tggcgtacac ggcgctcggc acctggctca cggtgaaggt
     6481 gtttggcgcg ccgctgatgc gcctgaactt ctttcagctc aagcgcgagg cggacttccg
     6541 cttcagcctg gtgcgggtgc gcgaaaacgc cgaatcgatt gccttttacc gcggcgagcg
     6601 ggccgagctg aagcaggtgc gcggccgctt caaaggcgcg ttgcgcaact accagcggct
     6661 gctgcgccag caactcaacc tcaacttctt tcagcaagcg tacaaccaga ttgccatcat
     6721 cctgcccaac atcctgatcg ccaacctggt gctgtcgggc cagctcgaag tgggccaggc
     6781 catcaaggcg gcgggcgcct ttctggcggt gctgggcgcg gtgtcgatca tcatcgacaa
     6841 cttcgaaggc ctgtcgcgct tcaccgccgg catcgagcgc atccacggcc tgctggcggc
     6901 gctgcccgcg cagccgggcg agagcgacga ggcggtagcg gccgaaccgc cacccctggc
     6961 gcgccgccgg cgccacacca tcgaaaccac cgaggcgccc gaactcagct tgcaaggcgt
     7021 aacgctcaac acgcccgacg gcaagcgcga gctggtgcgc gatctgagct tcacgctgca
     7081 caagggcgac gggctgctga tcgtcggcga aagcggcagc ggcaagagtt cgctgctgcg
     7141 cgccattgcc gggctgtggc gttcgggcga aggcagcatc cagcgcccgc cggtggccga
     7201 cagcatgttc ttgccgcaac gcccctacat gcaggtgggc agcctgcgca gccagctgct
     7261 gtacccgcag ttcgaccgcg aactggacga cgcggtgctg catcaggcac tgcacgatgt
     7321 gaacctgccc gatctggtgc agcgcagcgg cgggctcgac accgaggtcg actgggccaa
     7381 gactctgtcg ctgggcgagc agcaacgcct ggcctttgcc cgcgtgctga tcagcgcgcc
     7441 acgcttcgtc atcctcgacg aagccaccag cgccctcgac gccgccaacg aggcgcgcct
     7501 gtaccgccgc ctgctggccg aagacaccac gctgatcagc atcgcgcacc gcgccgccgt
     7561 gctcgaccac cacacgcacg tgctggagtt gaccggcggc ggccgctggg cgctgcacca
     7621 ggcggaaggt tttgcgttcg ccgaagggcc aagttcgcgc tcatcggcgg ccgataccgc
     7681 agccaccggc gacggcgctg caccagcaaa ctagcccggt gcggcgcgcg cggccagcgc
     7741 gtaacatggc cccacgcccg caccgcgcgg gccgcgcccc acttctgccg gagccctgtc
     7801 gatgaaaccg ctgtccgccg ccaccttgct gcttgcctcc ctgctctcca cgcccatcac
     7861 cgcactggcg gcgctgaaag ccggcgacgc cgcgccgccg ttcaagacgc cggccgcggt
     7921 ggccggccag gcgttcgact tcgacctgag cgccgcgctg aagaaaggcc cggtggtgct
     7981 ctacttcttc cccaaggcgt tcaccaaggg ctgcaccgtc gaggccaacg cgtttgccga
     8041 agccacgccg cagttcgccg cgctgggcgc caccgtggtc ggcatttcgc acgacgacat
     8101 cgacacgctg aaaaaattca gcaccgagga atgccgcaac aagtttgccg tggcatccga
     8161 ccccaaagcc ctgaccatca aggcctacga cgccggctcg ctgctgcccg gcatggccag
     8221 ccgcatctcg tacgtcatcg ggccagacgg caagatcgcc ttcacccacg aaggcagcaa
     8281 cccgatcaag cacgtcgagc agacgctgga ggcggtgcgg cgcttgaaga agtagcatgg
     8341 cagcgcgcaa gcggcgacgc cttgcaagcc agaatctgat caaaaacgcc gccagcgctt
     8401 gtccaacaag cgcaagcagc tatcaatagc atagtcaggc ggttcaggtc acgtcatcgc
     8461 cccgctcgct cggcgcgccg ggcgcgctgg gcggcatgcc gcgccacgaa tcgcggttgc
     8521 gcagcaccac ggtgatctgg ccattggtct cgatggtggc gcgctccacc tcgtgcgtgt
     8581 gcaggcagcc ggcggcgcgc agcgcggcgt gcacgtcgtc ggcggtcagc cgctcggcgc
     8641 gcatcaggtt ttcatccagc gcgccggtgc ggatcagcac ctgcggcttg ccatcaatca
     8701 ggcgcgccag gcgcttcgag cgccacatca ggtgcgccat gaccacgtgg cagccgatca
     8761 gcgtcaacgc cgagatcagg ccgccgatca gcgagttgtc gcccgcgttc atcgagttct
     8821 gcaccgcgtt cgacaggatc agcagcagga tcaggtcaaa cgggtcgtac tgcccgatct
     8881 gccgccgccc gctgatgcgc aggaaaaaca gcaggaacag gtagaccacc acgccgcgaa
     8941 cgacgaattc ccaccacggc acggacatat cgaacatggg tcggctccgg aaagttgtgc
     9001 gcgcattgtg cggccggcat tcgcgcgtgg tcaccgtgtt ggccgcgccg tccgacagcc
     9061 gccgtcggcc accaccgaca cccgccacgc ccagacgccg caaagatcaa ggggtgttca
     9121 tcaaagaagt ccgtgcgccc accgagcgcc tgaggagccc cgccatgccc atccgcaccc
     9181 gccatttctc ccccgccctc gtcattgccg ccgcgttgct ggccgcctgc ggccagaagg
     9241 acgccggcac cccccaaatc cccgcaccgg cgggcgaagc catgaccaag gccatcgagg
     9301 cgcagggcgc cgccaatcag gcggcagccg ccaccgcatc ggccgccagc gccgctgcgg
     9361 acgccagcca aagcgcggcc gccgccggca ccagcaccag cgacgcacaa gcggccgccg
     9421 ccgctgcagt tgccgccgcc accatggccg catcggccgc gaccgaggcg aagcaggcca
     9481 ccgccgacag cgctgcggcg cccgcatcgg cggccagtca gtaaaaacga aatcagccga
     9541 agccgcgcgt caggcttcgg acagcagccg cccgaggatc gcgcccagcg gcaagtccgg
     9601 cacatgtccg aagtggcgca aacgcaagcg cccttgccga tccaccacca gcgtggtcgg
     9661 cgtgccctgc aagccccagc gcgccatggt gcgcgggaat ggtgcgacat ccgctgaagg
     9721 cgcatcgacg ccgaccggaa aacgcagacc gtattcgtgg atgaaggccg ccagcgcgtg
     9781 cggctgcatg gcgtcgtggt gctcgaacac cgtgtgcagg ccaatcatcg ccacgccttg
     9841 atcggcaaac tgctcatgca cttggcgcgc ctgcggaatg gcgtg
//
LOCUS       DAOMVO010000062         9766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1735_length_9766_cov_12.535269, whole genome shotgun sequence.
ACCESSION   DAOMVO010000062 DAOMVO010000000
VERSION     DAOMVO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_9766_cov_12.535269"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKV30_04790"
     CDS             <1..375
                     /locus_tag="PKV30_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL29570.1"
                     /translation="ELSRVLGNLLENARRYGQSTDSGITRVRIAATARDQWVTLRVRD
                     HGPGVSPDLLPNLTRPFFRGDTARTSATGAGLGLAIVAKMVHNMGGALELANSPSGGL
                     MAVIRLQQATESRQGSLLKKRR"
     gene            complement(434..934)
                     /gene="ispF"
                     /locus_tag="PKV30_04795"
     CDS             complement(434..934)
                     /gene="ispF"
                     /locus_tag="PKV30_04795"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRL29571.1"
                     /translation="MTANQATLPPFRIGEGWDVHALVPGRPLVIGGVTIPHTHGLLGH
                     SDADVLLHAITDALLGAAGLGDIGTHFPDTDDRFAGADSHALLAEGVRRVAASGWRIG
                     NVDSTVIAQAPRLAPHIGAMRARIADALGVAQAQVNIKAKTAEKLGPVGQGASIEARA
                     VVLIFQ"
     gene            complement(931..1653)
                     /gene="ispD"
                     /locus_tag="PKV30_04800"
     CDS             complement(931..1653)
                     /gene="ispD"
                     /locus_tag="PKV30_04800"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRL29572.1"
                     /translation="MAAPMPSPAPPVRCHALLPCAGSGSRAGTALPKQYQPIAGQPMV
                     RHTLAAFAQVKRIAQVWVVVSPGDDTLRPSNAAFSIAACGGVTRAESVFNGLNHLLAN
                     GAAPSDWVLVHDAARCLVTPAQIDALIDACLPDGVGGLLALPLSDTLKHAGADGRVDA
                     TIDREGKWLAQTPQMFRIGALRDAYAAHAASAFAGITDEAIAMEAAGHAPLLVRGSAQ
                     NFKVTYPEDFALAEAILNSRNA"
     gene            complement(1736..>2515)
                     /locus_tag="PKV30_04805"
     CDS             complement(1736..>2515)
                     /locus_tag="PKV30_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRL29573.1"
                     /translation="VPIEQVRVVLGDTDRGNGFGSAGSRSLFTGGSAMRSGADKTLDE
                     AKQLAAKALEAAPEDIRYEAARFVVVGTDHAIGLFELAAKQDGGPIFVDHTHTVSGPT
                     WPNGCHISEVELDPETGTIAIVAYASVNDVGRVVNPMIVRGQLDGGAVQGIGQALTEQ
                     IVYDDSGQLLTGSLMDYAAPRALDMRTMFKTEMDESTPCTNNPLGVKGVGELGTIGAT
                     PSIVNAVADALARGGRADLAPRLQMPLSPGRVWALLQSTEA"
     assembly_gap    2516..2615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2616..3793)
                     /locus_tag="PKV30_04810"
     CDS             complement(<2616..3793)
                     /locus_tag="PKV30_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRL29574.1"
                     /translation="MSNATSPLNPQRFGSGQAVRRLEDDALLRGQGRYTDDLGEPGDG
                     RLVFVRSPYAHATIKGVDAEAARAMPGVRAVITGADLAAMPPLPTGAGFKRADGSDCA
                     SPVRRALAHEAVRYVGEAVVAVVADTLQQAKDAADSVMIDYDELPAVPTLAAALAADA
                     AVTEAAPDNIACEARHGDAAAAQAAFDQAAHIVKLNITHQRLAALTLEPRALRAAWDG
                     ARLDVHASSQMPTAVRGAIATALALKPEDVRVRVGDVGGGFGMKTGSYPEDVVIAWAA
                     RATAKPVRWVGERGEEFLSTTHGRDVQAHAELALDAGGKILALRVHSHANVGAYPTGT
                     GVAIQLLIGPWVQTSVYHVPVIDFHFKAVLTNQAPTGAYRGAGRPEAIFNIERLMDEA
                     A"
     gene            4010..7468
                     /gene="mfd"
                     /locus_tag="PKV30_04815"
     CDS             4010..7468
                     /gene="mfd"
                     /locus_tag="PKV30_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRL29575.1"
                     /translation="MDLPKLIPGKRHALPRPPASSDALLLAQLAEREAGQKKLTAIVT
                     ADAGDARRLMDEMGFFAPGLRSVMFPDWETLPYDTFSPHQDLISERLATLWAIRQQQA
                     DVVLIPATTALYRLAPPGFMAGYTFHFKQGQKLDEAALKSQLTLAGYTHVTQVVSPGE
                     YAVRGGLIDLFPMGSALPYRVDLFDDEIDSIRTFDADSQRSLYPVPEVRLLPGREFPM
                     DEPARAKFRHRWRELLEGDPTKSRIYKDMGNGVATAGIEYYLPLFFDDTATVFDYLST
                     DATLVLHGDLEGAFQQFAQDTRERYRLAQGDPARPVLPPEALFLDAEQFYLQAKPHAQ
                     LALRPAVADIAESAFAQKLPELTVVRGADDPLSRLQAHLRASPQRALVLAESDGRRES
                     LLDFLRASGLNPPAFDSLAEFQASDEPIGIATAAVAQGFAWLEQGIDFVTETELFAQG
                     ATTRRRRKQEQVSDVEALIKDLSELNVGDPVVHAQHGIGRYRGLVEMDMGAKNPDGSP
                     ACQEFLHLEYAADAVLYVPVSQLHLIGRYTGVSADEAPLHKLGSGQWEKAKRKAAEQI
                     RDAAAELLNIYARRAAREGHPFRYSPHDYEQFAADFGFEETADQQAAIHAVVQDMISP
                     QPMDRLVCGDVGFGKTEVALRAAFVAVTGGKQVAFLAPTTLLAEQHYRTLVDRFAKWP
                     VKVAEMSRFRSTKEINAALKGVADGSVDIVVGTHKLLSEKTQFKNLGLLIIDEEHRFG
                     VRHKEQMKQFRAEVDVLTLTATPIPRTMGMALEGLRDLSVIATAPQRRLAIKTFVRNE
                     GTGVIREAVLRELKRGGQVYFLHNEVETIENRRQALEKILPEARIAIAHGQMPERELE
                     RVMRDFVAQRHNLLLCSTIIETGIDVPTANTIVISRADRFGLAQLHQLRGRVGRSHHQ
                     AYAYLMVPEIESLTKQAAQRLEAIQQMEELGSGFYLAMHDLEIRGAGEVLGENQSGMH
                     IEIGFQLYNEMLAEAVKALKAGREPDLLSPMQAVTEINLHAPALLPGDYCGDVHLRLS
                     FYKKLATAKTSEQIDALLEEIVDRFGKLPPQAQTLIDVHRLRVLSQPFGVQKVDAAPG
                     VISISFKPGAPIDPMAIIQMIQKNKHIKLAGNDKLRIERELPDPKARAQMVRDVLRSL
                     RAPQPA"
     gene            7619..7939
                     /locus_tag="PKV30_04820"
     CDS             7619..7939
                     /locus_tag="PKV30_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29576.1"
                     /translation="MRKLKSVALLAVAAALSSPVLAQDDPRQSAQNSDTSMPNSPFKY
                     KGFPDAGEFNPTGRYVPPPAPVVVVSPPPPVVVPPPPPPPVVTPPPPPPPPPEPRPPR
                     PDRG"
     gene            8083..8796
                     /locus_tag="PKV30_04825"
     CDS             8083..8796
                     /locus_tag="PKV30_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRL29577.1"
                     /translation="MPLLYPSHVATPGRLKTALGLSALTVALQVSAASPADAAANASP
                     GAVVSSVAERFAQDVVRTSDAGGRTFGIIDKPSATLWVFDAQGRALAGTPVLVGEATG
                     DVAPADIGTRPLSRVKKHEKITAAGRYITEAGNNHKSEDIVWLDYDAALSMHRVRNVP
                     GEGRAKRLQTPTVADNRISFGCVNIPASFYDRYIDPLFSRTSGVVYVLPETKSMASVF
                     PFATEGGAALASVAPGPTH"
     gene            8906..9643
                     /gene="serB"
                     /locus_tag="PKV30_04830"
     CDS             8906..9643
                     /gene="serB"
                     /locus_tag="PKV30_04830"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRL29578.1"
                     /translation="MNTASLSDSAREFSPGLSVRGIAPPLRLRDFKLIAFDMDSTLIN
                     IECVDEIADAAGRKAEVAAITEAAMRGEITDYKESLRQRVAMLQGVREEHLAQVYRAR
                     LRLNPGAAELVLACKAAGLKVLLVSGGFTYFADRLRDRLALDFARSNLLEIVGGQLTG
                     RILPQSWGDICDGAMKRETVLRTCSLIGCEPAQAIAMGDGANDLEMMGAVGLSVAYRA
                     KPRVREQAKVAIDMGGLDRLLELFGEA"
BASE COUNT         1642 a         3328 c         3194 g         1502 t
ORIGIN      
        1 gagctgtcgc gcgtgctggg caacctgctg gagaacgcgc gccgctacgg ccagagcacg
       61 gacagcggca tcacgcgcgt gcgcatcgcc gccaccgcgc gcgaccaatg ggtcacgctg
      121 cgcgtgcgcg accatggccc cggcgtgtcg cccgacctgc tgcccaatct gacgcgcccc
      181 ttcttccgtg gcgacaccgc gcgcacctcg gccaccggcg ccggcctggg gctcgccatt
      241 gtggccaaga tggtgcacaa catgggcggc gcgctggagc tggccaacag cccaagcggt
      301 ggcctgatgg ccgtcatccg cctgcagcag gccactgagt cgcggcaggg ctcgctgctg
      361 aaaaagcggc gctgaaatcc gccgaaaaat tgattaaata cggccccaga gattgcagca
      421 tctgcgcaag cagctactga aagatgagca cgacggcgcg cgcctcgatg ctcgcgccct
      481 gccccactgg ccccagtttt tcagccgtct tggccttgat gttgacctgg gcctgagcca
      541 cacccagcgc gtcggcgatg cgcgcgcgca tggcgccgat gtgcggggcc agtcgcggcg
      601 cctgcgcgat gaccgtgctg tcgacgttgc cgatgcgcca acccgaagcg gccacgcggc
      661 gcacgccttc agccagcaga gcatgcgaat cggcaccggc aaagcgatcg tcggtgtcgg
      721 gaaaatgcgt gccaatgtcg cccaaccccg ccgcgcccag cagcgcatcg gtgatggcgt
      781 gcagcagcac gtcggcatcg gaatgaccca gcagaccgtg cgtgtgcggg atggtgacgc
      841 cgccaatcac cagcggccgg ccgggcacca gggcgtgcac gtcccagcct tcgccgatgc
      901 ggaaaggggg cagggttgct tggttggcgg tcatgcgttt cgtgagttca atatggcttc
      961 ggccagcgcg aagtcttcgg ggtaggtgac cttgaagttc tgcgcgctgc cgcgcaccag
     1021 cagcggcgca tggccggccg cttccatggc gatggcctcg tcggtgatgc cggcaaaggc
     1081 gctggccgcg tgcgccgcgt acgcatctcg cagcgccccg atgcggaaca tctgcggcgt
     1141 ttgcgccagc catttgcctt cgcggtcgat ggtggcatcc acgcggccat cggcacccgc
     1201 gtgcttgagc gtgtcggaca gcggcagcgc caacaggcct cccacgccat cgggcaggca
     1261 ggcgtcaatc aacgcgtcga tctgcgccgg cgtgaccaag cagcgcgcgg cgtcgtgcac
     1321 cagcacccag tcggacggcg cggcgccgtt ggccagcaag tgattcaagc cattgaaaac
     1381 gctctcagcg cgcgtcactc caccgcaggc agctattgaa aacgcagcat tcgatgggcg
     1441 caacgtgtca tcccccggcg acaccaccac ccacacctgc gcaatgcgct tcacttgggc
     1501 aaacgctgca agcgtgtgcc gcaccatggg ctgacccgct atcggttgat attgtttcgg
     1561 caaggcagtg ccggcgcgcg aaccgctgcc cgcgcagggc agcagggcgt ggcagcgaac
     1621 gggtggtgca ggcgaaggca tcggcgccgc cattgtagtg agccgcgggt gccgatggac
     1681 tccgttttca atagctgctc gcgcttgcca gaccagcgcg aacggccgat ttgattcaag
     1741 cttccgtcga ttgaagcaag gcccacaccc gccccggcga cagcggcatt tgcaagcgcg
     1801 gcgccaagtc agcgcgaccg ccgcgcgcca gcgcatccgc tacggcattg acgatgctgg
     1861 gcgtggcgcc gatggtgccg agttcgccga cgcctttcac gcccagcggg ttgttggtgc
     1921 acggggtgga ttcgtccatc tcggtcttga acatggtccg catgtcgagg gcgcgcgggg
     1981 cggcgtagtc catcaggctg ccggtcagca actggccgct gtcgtcgtag acgatttgct
     2041 cggtcagcgc ctggccgatg ccttgcaccg cgccgccgtc gagctggccg cgcacgatca
     2101 tcgggttgac cacacgaccg acatcgttga cggatgcgta ggcgacgatg gcgatggtgc
     2161 ccgtctcggg gtcgagttcg acttcgctga tgtggcagcc gttgggccag gtggggccgc
     2221 tcacggtgtg cgtgtggtcg acaaagatgg ggccgccgtc ttgctttgcc gccagctcga
     2281 acaggccgat ggcgtggtcg gtgccgacga cgacaaagcg cgcggcttcg tagcggatgt
     2341 cttccggcgc ggcttccagt gccttggcgg ccagttgctt ggcctcgtcc agcgtcttgt
     2401 cggcgcccga gcgcatggca gagccgccgg taaacagcga gcgcgaaccc gcgctgccaa
     2461 agccgttgcc gcgatccgtg tcgcccagca ccacgcgcac ctgttcaatc ggcacnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcggc ttcgtccatc agccgctcga
     2641 tgttgaagat cgcctcgggc cggccggcgc cgcggtacgc gccggtgggc gcctgattcg
     2701 tcagcacggc tttgaaatga aagtcgatca ccggcacgtg gtacacgctg gtctgcaccc
     2761 aggggccgat cagcaactga atcgccacgc cggtgcccgt ggggtaggcg cccacgttgg
     2821 cgtgcgagtg cacgcgcagc gcgaggattt tgccgcccgc gtccagcgcc agttcggcgt
     2881 gcgcctgcac gtcgcgcccg tgcgtggtgc tgagaaattc ctccccgcgc tcgcccaccc
     2941 agcgcacggg cttggccgtg gcgcgcgcgg cccacgcgat gaccacgtcc tcgggatacg
     3001 agccggtctt catgccaaag ccgccgccca cgtcgcccac gcgcacgcgc acgtcttcgg
     3061 gcttgagcgc cagcgcggtg gcgatggcgc cgcgcacggc cgtgggcatt tgcgagctgg
     3121 cgtgcacgtc gagccgcgcg ccatcccacg cggcgcgcag cgcacgcggc tccagcgtga
     3181 gcgcggccag gcgctggtgc gttatgttca gcttgacgat gtgcgcagcc tggtcgaatg
     3241 ccgcctgcgc ggccgctgcg tcgccgtggc gcgcctcgca ggcaatgttg tcgggcgcgg
     3301 cctcggtcac cgccgcgtcg gccgccagcg ccgcggccag cgtgggcacg gcgggcaact
     3361 cgtcgtaatc gatcatcacg ctgtcggccg cgtccttggc ttgctgcagc gtgtcggcca
     3421 ccacggcgac caccgcctcg cccacgtagc gcaccgcctc gtgcgccagc gcgcggcgca
     3481 cgggcgatgc gcagtcgctg ccgtcggcac gcttgaagcc agcgccggtg ggcaacggcg
     3541 gcatggcggc caggtcggcg ccggtgatca ccgcgcgcac gccgggcatg gcgcgcgcgg
     3601 cctcggcgtc cacgcccttg atggttgcgt gcgcataggg cgaacgcacg aagaccagcc
     3661 ggccatcgcc cggctcgccc aagtcgtcgg tgtagcggcc ctggccgcgc agcagcgcat
     3721 cgtcttccag ccgcctgacg gcctggccgc tgccgaaacg ctgcgggttc aacggggagg
     3781 tggcattgct catggggcgc tccggcaact aggggtggtg ggtggcagaa actatagacg
     3841 gcttgccgat gcagcgcccg gcgcttttat tttgatagct gccagcgctt gccagacaag
     3901 cgctggcggc caaaaatgct tgaaacacgg cggacgtaaa ataagcgcca cacccccttc
     3961 cacgctggcc gggggttttt gtcgtttctg ccgccttcgg atttctttca tggatttgcc
     4021 caaactcatc cctggcaagc gccacgccct gccgcgcccg cccgcgtcaa gcgacgcgct
     4081 tttgttggcg caactggccg aacgcgaagc cggccagaaa aagctcaccg ccatcgtcac
     4141 cgccgacgcg ggcgacgcgc ggcgcttgat ggacgagatg ggcttcttcg cgcccggcct
     4201 gcgcagcgtg atgtttcccg actgggagac gctgccctac gacaccttca gcccgcacca
     4261 ggacttgatc agcgagcggc tggccacgct gtgggcgatt cgccagcagc aggcggatgt
     4321 ggtgctgatc cccgccacca cggcgctgta ccggctggcg ccgccgggtt tcatggcggg
     4381 ctacaccttc cacttcaagc aagggcaaaa gctggacgaa gcggcgctga aaagccaact
     4441 cacgctggcc ggctacacgc acgtcacgca ggtggtcagc ccgggcgagt acgcggtgcg
     4501 cggcgggctg atcgacctgt tccccatggg cagcgccctg ccctaccggg tggatttgtt
     4561 cgacgacgag atcgactcga tccgcacctt cgacgccgac agccagcgca gcctgtaccc
     4621 ggtgcccgaa gtgcgcctgc tgcccggccg cgaattcccc atggacgagc cggcgcgcgc
     4681 caagtttcgc caccgctggc gcgagctgct ggagggcgac ccgaccaaga gccgcatcta
     4741 caaggacatg ggcaacggcg tcgccaccgc cggcatcgag tactacctgc cgctgttctt
     4801 tgacgacacg gcgacggtgt tcgactacct gagcacagac gccacgctgg tgctgcacgg
     4861 cgatctggaa ggcgcgtttc agcagttcgc gcaagacacg cgcgagcgct atcggctggc
     4921 gcagggcgac ccggcgcggc ccgtgctgcc gcccgaggcg ctgtttctgg acgccgagca
     4981 gttctacctg caggccaagc cgcacgcgca actggcgctg aggccggccg tggcagacat
     5041 cgcagaaagc gccttcgcgc agaagctgcc cgagctgacc gtggtgcgcg gcgccgacga
     5101 tccgctctcg cgcctgcaag cccacctgcg cgccagcccg cagcgtgcgc tggtgctggc
     5161 cgaaagcgat ggccggcgcg agagcctgct ggactttttg cgcgcctcgg ggctcaaccc
     5221 gccggccttc gattcgctgg ccgagtttca ggccagcgac gaacccatcg gcatcgccac
     5281 cgccgccgtg gcgcagggct ttgcctggct ggagcaaggc atcgacttcg tcaccgagac
     5341 cgagctgttt gcccaaggcg ccactacgcg ccggcgccgc aagcaggagc aagtgagcga
     5401 tgtcgaggcg ctgatcaagg atctgtcgga gctgaacgtg ggcgacccgg tggtgcacgc
     5461 gcagcacggc atcggccgct atcgcggcct cgtcgagatg gacatgggcg ccaaaaaccc
     5521 cgacggctcg ccggcgtgcc aagaatttct gcaccttgaa tacgccgccg acgccgtgct
     5581 gtacgtgccg gtgtcgcaac tgcacttgat cggccgctac accggcgtca gcgccgacga
     5641 agcgccgctg cacaagctcg gcagcggcca gtgggaaaaa gccaagcgca aggcggccga
     5701 gcaaatccgc gatgcggcgg ccgagctgct caacatctac gcccgccgcg ccgcgcgcga
     5761 aggccacccg ttccgctaca gcccgcacga ctacgaacag ttcgcggccg acttcggctt
     5821 tgaggaaacc gccgaccagc aagcggccat ccacgccgtg gtgcaggaca tgatcagccc
     5881 gcagccgatg gaccgtctgg tgtgcggcga tgtgggcttt ggcaagaccg aagtcgcctt
     5941 gcgcgcggcc tttgtggccg tcaccggggg caagcaggtg gcctttctgg cgcccaccac
     6001 tctgttggcc gagcagcatt accggacact ggtcgaccgc tttgccaagt ggccggtgaa
     6061 ggtggccgag atgagccgct ttcgcagcac caaggaaatc aacgcggcgc tgaaaggcgt
     6121 ggccgatggc agcgtcgaca tcgtggtcgg cacgcacaag ttgctgagcg agaaaacgca
     6181 gttcaagaac ctgggcctgc tcatcatcga cgaagagcac cgctttggcg tgcgccacaa
     6241 ggagcagatg aagcagttcc gcgccgaggt ggacgtgctg acactcaccg ccacgccgat
     6301 cccgcgcacc atgggtatgg cgctggaggg cttgcgcgac ttgagcgtga tcgccaccgc
     6361 gccccaacgg cgcctggcga tcaagacctt tgtgcgcaat gaaggcacgg gcgtgatccg
     6421 cgaagccgtg ctgcgcgaat tgaagcgcgg cgggcaggtc tactttctgc acaacgaagt
     6481 agagaccatt gagaaccgcc gccaggcgct ggaaaaaatc ctgcccgagg cgcgcatcgc
     6541 catcgcccac ggccagatgc ccgagcgcga actggagcgc gtgatgcgcg actttgtggc
     6601 gcagcgccac aacctgctgc tgtgctcgac catcatcgag accggtatcg acgtgcccac
     6661 ggccaacacc atcgtcatca gccgcgccga ccggtttggc ctggcgcagc tgcaccagct
     6721 gcgcggccgc gtggggcgca gccaccacca agcctacgcg tatttgatgg tgccggaaat
     6781 cgagagcctg acgaagcagg ccgcgcagcg gctggaagcc atacagcaga tggaggaact
     6841 gggcagcggc ttttacctgg ccatgcacga cctggagatt cgcggcgccg gcgaggtgct
     6901 gggcgaaaac cagagcggca tgcacatcga gatcggcttt cagctctaca acgagatgct
     6961 ggccgaggcg gtgaaggcgc tgaaagccgg gcgcgagccc gacttgcttt cgcccatgca
     7021 ggccgtgacc gagatcaacc tgcacgcgcc tgccctgctg ccgggcgact attgcggcga
     7081 tgtgcatttg cgcctgtcgt tctacaagaa gctggccacc gccaagacca gcgagcagat
     7141 cgacgcgctg ctggaagaaa tcgtcgaccg cttcggcaag ctgccgccgc aggcgcaaac
     7201 gctgatcgac gtgcaccgcc tgcgcgtctt gagccagccg tttggcgtgc agaaggtgga
     7261 cgcggcgccg ggcgtcatca gcatcagctt caagccgggt gcgcccatcg accccatggc
     7321 catcatccag atgatccaga agaacaagca catcaagctg gccggcaacg acaagctgcg
     7381 catcgagcgt gagttgcccg accccaaggc ccgcgcgcaa atggtgcgcg acgtgctgcg
     7441 cagcctgcgc gccccccagc ccgcctgacg ccgcgcgcgg gcagcgtcct acgcgcgaag
     7501 ccgccgccag tggacagtgg gcggccgcgc ggcctcctaa agttcgattc aggctttgcg
     7561 tcaacgagat gtgcccagcg gggcgcgagc ccttcgtaaa aacttcaagg agtgatccat
     7621 gagaaaactg aagtctgtgg ccctgctggc cgtggccgca gcgctgagca gcccggtgct
     7681 cgcccaagac gacccgcgcc agagcgccca gaacagcgac acctcgatgc cgaactcgcc
     7741 gttcaagtac aaaggctttc cagatgccgg cgagttcaac cccaccggcc gctacgtgcc
     7801 gccgcccgcg cccgtcgtgg tggtgagccc gccgcccccg gtcgtggtgc cgccgccgcc
     7861 cccgccgccc gtggtgacac cgccgccgcc gccgcctccc ccgccggagc cgcgtccgcc
     7921 acgccctgac cgcgggtaac caagcccgtc ggttgaagca aaaaagcctc ggcctgcccg
     7981 aggctttttt gtcacattgg cggcgtttgg gtgtcccttg gggttcactg cccgtcaatc
     8041 gatcgtataa acagcttttc agaccttgca ttacccaccg ccatgccgct gctttatcca
     8101 tctcatgtcg ccacgccggg ccggctgaaa accgcgttgg gtctgtccgc actgaccgtc
     8161 gcgctgcagg tcagcgccgc cagccccgcc gatgccgcgg ccaacgccag cccaggcgcc
     8221 gtggtgtcgt cggtggccga gcgctttgcg caggacgtgg tgcgcacgtc cgacgccggc
     8281 ggccgcacct tcggcatcat cgacaaaccc agcgccacgc tgtgggtgtt cgacgcccag
     8341 ggccgcgcac tggccggcac gcccgtgctg gtaggcgagg ccacgggcga cgtggcgccg
     8401 gccgacatcg gcacgcgccc gctgtcgcgc gtgaagaagc acgaaaagat caccgccgcc
     8461 ggccgctaca ttaccgaagc cggcaacaac cacaagtccg aagacatcgt gtggctcgac
     8521 tacgacgcag ccctgtcgat gcaccgcgtg cgcaacgtgc cgggcgaagg ccgcgccaag
     8581 cgcctgcaaa cgcccaccgt ggccgacaac cgcatctcgt ttggctgcgt caacatcccg
     8641 gccagctttt acgaccgcta catcgatccg ctgttcagcc gcacctcggg cgtggtctac
     8701 gtgctgcccg agaccaaatc catggccagt gtgttcccct tcgccaccga aggcggcgcc
     8761 gcacttgcca gcgtggcgcc tggcccgacg cattgagcgg gcgacgcggc tgccgcccgc
     8821 gcgccgtgga taatcgcggt ttcttgatct tttaagccgc tggcgcttgc ccatcaagcg
     8881 cgagcagcta tcaaaataaa agcagatgaa caccgcatcc ctttccgaca gcgcgcgcga
     8941 attcagcccc ggactcagcg tgcgcggcat tgcgccgccg ctgcgcctgc gcgacttcaa
     9001 gctgatcgcg ttcgacatgg attcaacgct gatcaatatc gaatgcgtgg acgagatcgc
     9061 cgacgccgcc ggccgcaagg ccgaagtcgc cgccatcacc gaagccgcca tgcgaggcga
     9121 gatcaccgac tacaaagaga gcctgcgcca gcgcgtggcc atgctccaag gcgtgcgcga
     9181 agagcatctg gcgcaggtct accgcgcccg cctgcgcctg aaccccggcg cggccgaact
     9241 ggtgctggcg tgcaaggcgg ccgggctgaa ggtgctgctg gtgtcgggcg gcttcaccta
     9301 tttcgccgac cggctacgcg accgcctggc gctggacttt gcgcgcagca acctgctgga
     9361 aatcgtgggt ggtcaactca ccggccgcat cctcccgcaa agctggggcg acatctgcga
     9421 cggcgccatg aagcgcgaaa ccgtgctgcg cacctgcagc ctgatcggct gcgagccagc
     9481 ccaagcgatt gcgatgggcg acggtgccaa cgacctggag atgatgggcg ccgtcggcct
     9541 gtcggtcgcc taccgcgcca agccccgggt gcgtgagcag gcgaaggtag cgattgacat
     9601 gggggggttg gatcggttgt tggagttgtt cggcgaggca tagcggagat aggggacgta
     9661 ggctggctcg gtcgcagcaa aggccagctt ccctgagtcc gggaacccca aatgcgccgc
     9721 tacatccgca cccacctctc gggcatcgcc tagagttcaa cgtaac
//
LOCUS       DAOMVO010000063         9745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1742_length_9745_cov_18.213209, whole genome shotgun sequence.
ACCESSION   DAOMVO010000063 DAOMVO010000000
VERSION     DAOMVO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_9745_cov_18.213209"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(516..1136)
                     /locus_tag="PKV30_04835"
     CDS             complement(516..1136)
                     /locus_tag="PKV30_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRL29579.1"
                     /translation="MRLIGAVTSPYVRKVRVVLAEKKLDYKFLPENVWADDTQIGAAN
                     PLGKVPCLVMDGQDAVFDSRVIVEYLDTLSPVGKLIPPSGRQRVEVKTWEALADGLMD
                     AAILARLERTWPGRAEGERSQAWIDRQMKKVDDALTAMSAGLADKPWCSQGVHLSLAD
                     IAVGCALGYLDFRFPELDWRGRHANLAHLLDDKLMQRPSFADTIPS"
     gene            1303..2706
                     /gene="purB"
                     /locus_tag="PKV30_04840"
     CDS             1303..2706
                     /gene="purB"
                     /locus_tag="PKV30_04840"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRL29580.1"
                     /translation="MTLTPLTAVSPLDGRYAGKLAALRPIMSELGYMHRRVQVELAWF
                     IALSDAGFDEFKPLSGEARAYLASLVRDFSEDDGASIKAIEKTTNHDVKAVEYWIKSR
                     FEGRPELEAAAEFVHFACTSEDINNTSHALQLKAGRVELLEHLDDVIARLRELAHAHA
                     AVPMLSRTHGQTASPTTVGKELANVVVRLAAARERIASVKLLAKMNGAVGNYNAHLSA
                     WPDFDWEAFSRRVVESPAAGEPGANAAKPGLGLTFQPYSIQIEPHDYMAELFDATARA
                     GTICIDLSRDIWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENAEGNLGLANAVLR
                     HLSEKLPISRWQRDLTDSTVLRNMGVALGYAVLAYQSLLTGLNKLELNEQALADDLDA
                     AWEVLAEPIQTVMRRFGVPGAYEKLKDATRGQAVTREALHALIRSLEIPQAEKDRLLA
                     LTPATYVGQAEALARRV"
     gene            2884..3444
                     /locus_tag="PKV30_04845"
     CDS             2884..3444
                     /locus_tag="PKV30_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HRL29581.1"
                     /translation="MTLKSTIFKADVQLADIDHGCYTDLQLTLARHPSETDERMMVRL
                     AALALNAHQVQDLCGGDAKIGFGAGLSSPDEPDVQLVDFTGRTRLWIEVGQLDDKPLA
                     KACSKADAVIVYAFGPAAEVWWRGIENKLTRLDKLQVWRIPAADAQALAGLAERSMAL
                     QATLQEGVLTLSSARGSVALEPLRWK"
     gene            complement(3655..3972)
                     /locus_tag="PKV30_04850"
     CDS             complement(3655..3972)
                     /locus_tag="PKV30_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRL29582.1"
                     /translation="MRRLTRAPNAAIAQIWADLLCEAGYPATVQRQFLSSVAGELPPD
                     QCLPEIWLRHDEHHAAARALLDELARVPQRRWQCPQCGEQIEGGFEQCWACGALMPSL
                     SNS"
     gene            complement(4046..4207)
                     /locus_tag="PKV30_04855"
     CDS             complement(4046..4207)
                     /locus_tag="PKV30_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29583.1"
                     /translation="MRFIRPLITCVGLLAAALSLAGCGATPHNPDTAGASGVTVYGTA
                     DAGIGRVRK"
     gene            complement(4283..5473)
                     /locus_tag="PKV30_04860"
     CDS             complement(4283..5473)
                     /locus_tag="PKV30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL29584.1"
                     /translation="MLLTLASAFTLSQAFRTLAAIMGPPLAAQLALTPQQLGLWAAAF
                     HFSFGVMQLAMGVSLDLYGVRRTILMAFPMAIAGALLSSQASSYAQLMVGQMLIGTGC
                     APAFLVCTVFISHHFSAQRFTAISGLIMSLSSIGVLATATPLAWLIEVSHWRWGFGVL
                     AGCAMLAWGAIWWLVREPAAATADAPGADKPSALGALRGLLGLFKLPQTLGLVAYAAV
                     AYAGFITLRGLWLGPLLVERHGFSLIQSGNVALAMTLASMASPALFGRIDPGGARRVR
                     WLLGFAVLAALMVGTMGLVHLAWADVALAIAYGLLSGYGVLQYGYVRDAYPAHLRGRA
                     LSLFTMAMFLGISLMQWASGLAASLAPRAGVEPFSAALLTMSALLLAGALAFWRLPRA
                     VTPA"
     gene            complement(5597..6592)
                     /locus_tag="PKV30_04865"
     CDS             complement(5597..6592)
                     /locus_tag="PKV30_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29585.1"
                     /translation="MTMKPLTRRSLFQALAAASLLGTTFAAQAAWPADKPIKLIVPFP
                     PGQATDIFARALAEPLAKKLGQSVVVENKAGAGSNIGSEFVARAAPDGYTLLVAGSAM
                     AVNQTLYKKVGFDPTKDLVGITGIAKVPLVFLAHPSAGIKTIPDLLAAARAKPGHLSY
                     ASAGIGGTQHLAAEMLKSQAKVFITHIPYRGSGPAQADFLGGQVPLMVDSVTAALPHI
                     QGGKVVPLAVTSATRSSQLPNVPTVAESGPKELRGFEAVGWLGLMAPKGTPPEVVQRL
                     NAEVVELLKGEALARFIRDRGSEPSPSTPAEFDRFVASEIVKWGAVVRASGASAD"
     gene            complement(6646..7119)
                     /locus_tag="PKV30_04870"
     CDS             complement(6646..7119)
                     /locus_tag="PKV30_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL29586.1"
                     /translation="MTTITKDAAVTIAYKMTDPQTGKPLDAGTTAYLHGHGNLFDKVE
                     AAIEGQPVGHSVTIDLAPTEAFGERDESLMRTIPKSEFPPGVKVGGQLQGAGPDGRTV
                     VYNVVKIKGPEVHLDGNHALAGHAIRFAATVKAIRPATAEELAHGHVHGAHGHHH"
     gene            complement(7126..7557)
                     /locus_tag="PKV30_04875"
     CDS             complement(7126..7557)
                     /locus_tag="PKV30_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL29587.1"
                     /translation="MVYVLDTNVVSELRLSRSRRSPAVHAWAQVQTDNQLFLSVVTKF
                     ELEMGELRIARRDAVQAARLQDWRLQLLAQFAGRILPFTELTTPFCAALHVPDPRSFR
                     DAMIAAAALEHGFTVVTRNVADFAGTGVHLVNPWESESSST"
     gene            complement(7564..7869)
                     /locus_tag="PKV30_04880"
     CDS             complement(7564..7869)
                     /locus_tag="PKV30_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRL29588.1"
                     /translation="MNAPISPGSLAAPITTYSSREFTRDVAAAKRAAANGPVFITDRG
                     APAYALVTIEEYHRLKRGGAKPRTLLEVMESLPDTSDIEFEPPKLMDIVKPFDFDAD"
     gene            complement(7935..>9745)
                     /locus_tag="PKV30_04885"
     CDS             complement(7935..>9745)
                     /locus_tag="PKV30_04885"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRL29589.1"
                     /translation="KRIFDNSKVSDHFAIIPTLEAPGVLSEAEQKLYDLVVRRFMAVF
                     YPSAEFMVTTRITTASHEGKAYNFQSNGKVLVRPGWLAIYGKEAAGETEGGGKDDKTL
                     VPVKPGEMVRNDHVDVKALQTRPPARYSEATLLGAMEGAGKWIEDDELREAMQEKGLG
                     TPATRAAIIEGLLNEKYMLREGRELIPTAKAFQLMTLLRGLQVEELSKPELTGDWEYK
                     LAQMEHGKLSRDSFMQEIAAMTERIVKKAKEYDRDTVPGDYATLQTPCPHCGGVVKEN
                     YRRYACTGKAGDGTGACGFSFTKTPAGRTFAVEEAEQFLRDKKIGPLEGFRSKAGWPF
                     VAELALVRDDESGNFKLEFDFGDDAKAAETGEIVDLSAEPAVGPCPKCGAPVKAFGKS
                     YVCTRSVPSHEHPVPSCDFKSGQIILQQPVEPEQMKKLLQDGKTDLLDKFVSMRTRRA
                     FKARLAWSAEEGKVLFEFEPREGARKYPPRKTAAAPAKAASKSGAAGASGAGATAKKA
                     AKKAPAKKAAPKAEKTPRAGNLKPSPALAAVIGEGPFGRGEVMKLLWEYIKAHNLQDP
                     KDKRTILADAKLKPVFGGEDSVGMFKLAGIVGGHLG"
BASE COUNT         1591 a         3251 c         3273 g         1630 t
ORIGIN      
        1 atccgaccac caagcggctg gtcgagcaga tcctgaacga cgagcaagag cacgccgagg
       61 aactcaagac ctggctggcc ctgtgatcgt gcgcggtggc gcgttggggc cgccgcagcg
      121 ataagaaggt cacggccggt acacacttct ttaccgcggc aggccggatg cggcggaact
      181 ctgcggcctt tcgaggcctc ataaccggag tgatgaacgg gactgacact cctgctgaat
      241 cttgcagctg ccgagcctga ggtttgaact tcaagccggt tgaaaaacct gaagcgcact
      301 tcctcctccc tccctcctcc tccttcgttt caggtcgctt cggcagcttt tttattctga
      361 acgcgccgcc cgggcaccgt gccttggcgg cgcttctttt tggggaacaa gccccagaaa
      421 tgactccacc ccgttcgtcc tgagcttgtc gaaggacttg gcgttgaggg agcaaggctt
      481 cgacaggctc agcccgaacg gcttgtggca aacgatcagc ttgggatggt gtccgcgaag
      541 ctcgggcgct gcatcagctt gtcgtccagc aagtgcgcca gattggcgtg ccgcccgcgc
      601 cagtccagct ccggaaagcg aaagtccagg taacccagcg cgcagccgac ggcaatgtcg
      661 gccagcgaca ggtgcacgcc ttgcgagcac cagggcttgt cggccaggcc agcgctcatc
      721 gccgtcagcg cgtcgtcgac cttcttcatc tgccggtcga tccaggcttg gctgcgctcg
      781 ccttccgcgc ggccgggcca ggtgcgctcc agccgcgcca ggatggcggc gtccatcagg
      841 ccgtcggcca gcgcttccca ggtcttgact tccacgcgct ggcggcccga aggcggaatc
      901 agcttgccca ccggcgagag cgtgtccagg tattcgacga tcacgcgcga atcaaacacc
      961 gcgtcctgcc cgtccatcac caggcacggc accttgccca gcgggttggc agcgccgatc
     1021 tgcgtatcgt cggcccacac gttctcgggg aggaacttgt agtccagctt cttctcggcc
     1081 agcacgacac gcaccttgcg cacgtagggg ctggtgacgg cgccgatcaa tcgcatggac
     1141 atggaatgtt caagaatgta acaacggttc agcccattct aggcgatggc ggcgacgcgc
     1201 cgtctcggcc atcggaggca agctgagcgg cgatgcggcc acccctcagc gcagtctaca
     1261 ggccgaaaca gcgcgccgtg cgcggctcct acaatttggc ccatgaccct gaccccgctc
     1321 actgccgttt ctccgctcga tggccgctac gccggcaaac tggccgcgct tcgcccgatc
     1381 atgagcgagc tgggctacat gcaccgccgc gtgcaggtcg agctggcttg gttcatcgcg
     1441 ctctcggatg cgggcttcga cgaattcaag ccgctgtcgg gcgaagcgcg cgcttacctt
     1501 gccagcctgg tgcgcgattt ttccgaggac gacggcgcgt ccatcaaggc gatcgagaag
     1561 accaccaacc acgacgtgaa ggcggtcgaa tactggatca agtcccggtt cgagggccgc
     1621 cccgaactgg aggccgcggc cgagttcgtg cacttcgcgt gcaccagcga ggacatcaac
     1681 aacaccagcc acgcgctgca gctcaaggcc gggcgcgtgg agctgctgga gcacctggac
     1741 gacgtcatcg cccgcctgcg cgagctggcg cacgcgcacg ccgccgtgcc catgctcagc
     1801 cgcacgcacg gccagacggc cagcccgacc acggtgggca aggagctggc caacgtggtg
     1861 gtgcgcctgg ccgccgcgcg tgagcgcatc gcttccgtca agctgctggc caagatgaac
     1921 ggcgccgtgg gcaactacaa cgcgcacctg tcggcgtggc cggacttcga ctgggaggcc
     1981 ttcagccgtc gcgtggtcga atcgcccgcc gccggcgagc cgggtgccaa cgccgccaag
     2041 cccggcctgg gcctcacctt ccagccctac agcatccaga tcgagccgca cgactacatg
     2101 gccgagctgt ttgacgccac ggcgcgcgcg ggcaccatct gcatcgacct gagccgcgac
     2161 atctggggct acatcagcct gggctatttc aagcaaaaga ccaaggcggg cgaaatcggc
     2221 tcgtccacca tgccgcacaa ggtcaatccc atcgacttcg agaacgccga aggcaacctg
     2281 ggcctggcca acgccgtgct gcgccacctg agcgaaaagc tgcccatcag ccgctggcag
     2341 cgcgatttga ccgacagcac cgtgctgcgc aacatgggcg tggcgctggg ctatgccgtg
     2401 ctggcgtacc agtcgctgtt gacgggcctg aacaaactcg aattgaacga gcaggcgttg
     2461 gccgacgacc tggacgccgc gtgggaagtg ctggccgaac ccatccagac cgtgatgcgc
     2521 cgctttggcg tgcccggcgc ctacgaaaag ctgaaagacg ccacgcgcgg ccaggcggtg
     2581 acgcgcgagg cgctgcacgc gctgatccgt tcgctggaga tcccgcaggc cgagaaagac
     2641 cgcctgctgg cgctgacccc cgccacctac gtcggccagg ccgaggcgct ggcgcggcgc
     2701 gtgtgaggct tcggtttttc aagccttttc ggcctccagc gcttgctgga caagcgccag
     2761 cagctatgat ttttgtaggg tgggtgcttg cacccaccgt gtttggcgac gcgctgcctg
     2821 cgtgggtgca agcacccacc ctacgtgatg ggcgcaggcc gctattgaac ttcgaataag
     2881 cccgtgaccc tgaaatccac catcttcaag gccgacgtgc agctcgccga catcgaccat
     2941 ggctgctaca ccgacctgca actcacgctg gcgcgccacc cgagcgagac cgacgaacgc
     3001 atgatggtgc ggctggccgc gctggcgctg aacgcccacc aggtgcagga cttgtgcggc
     3061 ggcgacgcca agatcggctt tggcgccggc ctgtcgagcc ccgacgaacc cgacgtgcaa
     3121 ctggtcgatt tcaccggccg cacgcgcctg tggatcgaag tcggccagct cgacgacaag
     3181 ccgctggcca aggcctgcag caaggccgac gcggtcatcg tctacgcctt tggccccgcg
     3241 gcggaggtgt ggtggcgcgg catcgagaac aagctgacgc ggctggacaa gctgcaggtt
     3301 tggcgcatcc ccgccgccga cgcgcaggcg ctggcgggcc tggccgagcg cagcatggcg
     3361 ctgcaggcga cgctgcaaga gggtgtgctc acgctgtcca gcgcgcgcgg cagcgtggcg
     3421 ctggagccgc tgcgctggaa gtgatcagcc ccgagtcttg agagccaatc ccgaaagcaa
     3481 cccagcaccc gtttgtcatg agccaatcgg caagctcatg acaggcttgt ggaaggactt
     3541 ggtggcgcgc aaagcggctt cgacaagctc agcccgaacg gatgatgatg tcgggaaatt
     3601 gtgtggctgc ctataaaaat cagccgccag cgcttgtcca gccagcgcaa gcagctatga
     3661 attcgataga gatggcatga gcgcgccgca cgcccagcac tgctcgaagc cgccctcgat
     3721 ctgctcgccg cactgcgggc attgccagcg ccgctgcggc acgcgcgcca gttcatcgag
     3781 cagcgcgcgg gcggcggcgt ggtgctcgtc gtgtcgcagc cagatttcgg gcaggcactg
     3841 gtcgggcggt agctcgccgg ccacgctgct caagaactgg cgctgcacgg tggccgggta
     3901 gcctgcttcg cacagcagat cggcccagat ctgggcgatg gcggcgttgg gggcacgggt
     3961 caggcggcgc atcaaaagtg gctttagcgc ttgaccggcg tgcgcgagca gctatgaaat
     4021 caaaagcaaa cgggtgttgt ggcgatcact tgcgcacgcg gccgatgccg gcgtccgcgg
     4081 tgccgtagac ggtgacgccc gaggcgccgg cggtgtccgg gttgtgcggc gtggcgccgc
     4141 agccggcaag cgacagcgcg gcggccagca ggccgacgca ggtgatgagg gggcggatga
     4201 atcgcatggc caaaaactcc ttcacacggt gcggccagta tgccccgcca cgccgccgcg
     4261 cgtgtaacgg cgggtgcggc gatcaagccg gcgtcacggc gcgcggcagc ctccagaacg
     4321 ccagcgcgcc cgccagcagc agcgcgctca tggtcaacag cgcggcgctg aagggctcca
     4381 cgccggcgcg cggcgccagg ctggccgcca gcccgctcgc ccattgcatg agcgatatgc
     4441 cgaggaacat cgccatggtg aacagcgaca gcgcgcgccc gcgcaggtgc gccgggtagg
     4501 cgtcgcgcac gtagccgtac tgcaacacgc cataacccga cagcagcccg taggcaatgg
     4561 ccagcgccac gtcggcccag gccaggtgca ccagccccat ggtgcccacc atcagcgccg
     4621 ccagcacggc aaagccgagc agccagcgca cgcgccgtgc gccgccgggg tcgatgcggc
     4681 caaacagcgc cgggctggcc atgctggcca gcgtcatcgc cagcgccacg ttgccgctct
     4741 ggatcagcga gaagccgtgg cgctcgacca ggagcggccc cagccacagc ccgcgcagcg
     4801 tgatgaagcc ggcatacgcc accgccgcgt aggccaccag tcccagcgtc tgcggcagct
     4861 tgaacaggcc cagcagcccg cgcagcgcgc ccagggccga tggcttgtcg gcgccgggcg
     4921 catcggccgt ggcggcggcc ggttcgcgca ccagccacca gatggccccc caggccagca
     4981 tggcgcagcc ggccagcacg ccgaagcccc agcgccagtg gctgacctcg atcagccagg
     5041 ccagcggcgt ggccgtggcc agcacgccga tgctgctcag gctcatgatc aggcccgaaa
     5101 tggcggtaaa gcgctgcgcg ctgaagtggt ggctgatgaa caccgtgcag accaaaaacg
     5161 ccggcgcgca gccggtgccg atcagcatct gcccgaccat caactgcgcg taactgctgg
     5221 cctgcgacga cagcagcgca ccggcaatcg ccatcggaaa cgccatcagg atggtgcgcc
     5281 gcacgccgta cagatcgagc gacacgccca tcgccaactg catgacgcca aacgaaaaat
     5341 ggaacgccgc cgcccacagc cccagctgct gcggcgtcag cgccagttgc gcagccagcg
     5401 gcggccccat gatggccgcc agcgtgcgaa acgcctggct cagcgtgaag gcgctggcca
     5461 gcgtgagcag catggcccaa gcctggcgcg aggtgagcgc gggcggcgcg gccgcagggg
     5521 cgatgtggga caacgggtgg ggcgactgca cggcggcagt ttacgggcgc gcggcgccgg
     5581 cgctgtcgcg cacgaatcag tcggcgctgg ccccgctggc gcgcaccacg gcgccccatt
     5641 tgacgatttc gctggccacg aagcggtcaa actcggccgg cgtgctgggc gaaggctcgg
     5701 agccgcggtc gcggatgaag cgcgccagcg cctcgccctt cagcagctcg accacctcgg
     5761 cgttcagccg ctgcaccacc tcgggcggcg tgcccttggg cgccatcagg ccgagccagc
     5821 cgacggcttc gaagccgcgc agctccttcg gcccgctctc ggccaccgtc ggcacattgg
     5881 gcaactgcga cgagcgcgtg gccgaagtga ccgccagcgg caccaccttg ccgccctgga
     5941 tgtgcggcag cgcggcggtg accgaatcga ccatcagcgg cacctggccg cccagaaaat
     6001 ccgcctgcgc cggcccgctg cctcggtagg ggatgtgggt gatgaacacc ttggcctggc
     6061 tcttgagcat ctcggccgcc aggtgctgcg tgccgccgat gccggcgctg gcgtagctca
     6121 gatgacctgg cttggcgcgc gcggcggcca gcagatcggg aatcgtcttg atgcccgccg
     6181 aaggatgcgc cagaaacacc aaaggtacct tggcgatgcc cgtgatgccg accagatcct
     6241 tggtgggatc aaagccgact ttcttgtaca gcgtctggtt gaccgccatg gcgctgccgg
     6301 ccaccagcag cgtgtagccg tcgggcgcgg cgcgcgccac gaactcgctg ccgatgttgc
     6361 tgccggcgcc agccttgttc tccaccacca cgctctggcc gagtttcttg gccagcggct
     6421 cggccagcgc gcgggcaaag atgtcggtcg cctggcccgg tggaaacggc acgatgagct
     6481 tgatcggctt gtcggccggc caggcggcct gggcggcgaa ggtggtgccg agcaggctgg
     6541 cggcagcgag cgcttgaaac aagctgcggc gcgtaagggg tttcatcgtc attttggcct
     6601 ctggtgcttg tccagcaagc gcgagcagct attaataaaa gagcgtcaat ggtggtgccc
     6661 gtgcgcgccg tgcacgtggc cgtgcgccag ctcttcggcc gtggcgggcc gaatcgcctt
     6721 cacggtggcg gcaaagcgga tcgcgtggcc ggccagcgcg tggttgccgt ccaggtgcac
     6781 ttcggggccc ttgatcttga ccacgttgta gaccaccgtg cgcccatccg gcccggcgcc
     6841 ctgcaactgg ccgccgacct tcacgccggg cgggaattcg ctcttgggga tggtgcgcat
     6901 cagcgattcg tcgcgctcgc caaaggcctc ggtgggcgcc agatcgatcg tcacgctgtg
     6961 gccgaccggc tggccttcaa tcgccgcctc gaccttgtcg aacaggttgc cgtggccgtg
     7021 caggtaggcg gtggtgccgg cgtccagcgg cttgcccgtc tgggggtcgg tcatcttgta
     7081 ggcgatggtg acggcggcgt ctttggtgat ggtggtcatg gtgtgtcagg tggatgagga
     7141 ttcagattcc catgggttca caaggtgcac gcccgtgccg gcaaagtcgg ccacgttgcg
     7201 ggtgacgacg gtgaagccgt gttcaagcgc ggcggcggcg atcatggcgt cgcggaaaga
     7261 tcgcgggtcg ggcacgtgca gcgcagcgca aaagggcgtc gtgagttcgg tgaagggcag
     7321 gatgcggcca gcgaactgtg ccagcaactg cagacgccag tcctgcagcc gcgctgcctg
     7381 cacggcatcg cgacgggcaa tgcgcaactc ccccatttcc agttcgaact tggtgacgac
     7441 cgacaggaac agctggttgt cggtttgaac ctgtgcccag gcgtgtaccg ccggtgagcg
     7501 ccgggaacgg ctcagccgca gctcggagac gacgttggtg tcgaggacgt acacgatggg
     7561 ccgtcagtcg gcatcgaaat cgaacggctt gacgatgtcc atcagcttgg gcggctcgaa
     7621 ctcgatgtcg ctggtatccg gcaggctttc catcacctcc agcagcgtgc ggggcttggc
     7681 accgccgcgc ttcaggcggt ggtactcctc aatggtcacg agcgcgtaag cgggcgcgcc
     7741 ccggtcggtg atgaacaccg gcccgttggc cgcggcacgc ttggcggccg ccacgtcgcg
     7801 cgtgaattcg cggctggagt aggtggtgat gggggcagcc aggctgccgg ggctgatggg
     7861 agcgttcatg gcgaatctcc tgcgtcgaac acgaatgctt gtatgttaga gcattcgcgt
     7921 ccgatgcgta cgcttcaccc caaatgcccc cccacaatcc cagccagctt gaacatgccc
     7981 acgctgtctt cgccgccgaa gacgggtttg agcttggcgt cggcgaggat ggtgcgtttg
     8041 tctttcgggt cttgcaggtt gtgggccttg atgtattccc acagcagttt catgacctcg
     8101 ccacggccaa aggggccttc gccgatgacg gcggccaggg cgggactggg cttgaggttg
     8161 ccggcgcgcg gggttttctc ggccttgggc gcggctttct tggcgggtgc ctttttagcg
     8221 gcttttttgg ccgtggcgcc cgccccgctt gcgccagcag ctcctgattt tgaagcggcc
     8281 ttggcaggcg ccgccgccgt cttgcgcggc gggtacttgc gcgcgccttc gcgcggctcg
     8341 aattcgaaga ggaccttgcc ctcttccgca ctccaggcca ggcgcgcctt gaaggcgcgg
     8401 cgggtgcgca tgctgacgaa cttgtcgagc aggtcggtct tgccgtcttg caggagcttc
     8461 ttcatctgct cgggctccac cggctgctgc aggatgatct ggccgctctt gaagtcgcag
     8521 ctgggcacgg ggtgctcgtg cgagggcacg ctgcgggtgc agacgtagct cttgccgaag
     8581 gccttgaccg gggcgccgca cttggggcac gggcccacag cgggttcggc cgacaggtcg
     8641 acgatctcgc ccgtttcggc cgccttggcg tcgtcgccaa agtcgaattc cagcttgaag
     8701 ttgccgctct cgtcgtcgcg caccagcgcc agttcggcga caaagggcca gccggccttg
     8761 ctgcggaagc cttccagcgg accgatcttc ttgtcgcgca gaaactgctc ggcttcctcg
     8821 acggcaaagg tgcgaccggc cggcgtcttg gtgaacgaga agccgcacgc gcccgtgcca
     8881 tcgcccgctt tgccggtgca ggcgtagcgg cggtagttct ctttcaccac gccgccgcag
     8941 tgcgggcacg gcgtttgcag cgtggcgtag tcgccgggca cggtgtcgcg gtcgtactcc
     9001 ttggctttct tgacgatgcg ctcggtcatc gccgcgattt cctgcatgaa gctgtcgcgg
     9061 ctcaacttgc cgtgttccat ctgcgccagc ttgtattccc agtcgccggt cagctcgggc
     9121 ttgctgagtt cttccacctg cagcccgcgc agcagcgtca tcagctggaa ggccttggcg
     9181 gtggggatca gctcgcgccc ttcgcgcagc atgtatttct cgttcagcag tccttcgatg
     9241 atggcggcgc gcgtcgccgg cgtgcccaag cctttctctt gcatggcttc gcgcagctcg
     9301 tcgtcctcga tccacttgcc ggcgccttcc atggcgccga gcagcgtggc ttcgctgtag
     9361 cgcgcgggcg ggcgcgtctg cagcgccttc acgtcgacgt ggtcgttgcg caccatctcg
     9421 ccgggtttca ccggaaccaa cgtcttgtcg tccttgccgc cgccttcggt ttcgccggcg
     9481 gcttccttgc cgtagatggc gagccagccc ggccgcacca gcaccttgcc gttgctctga
     9541 aagttgtacg ccttgccctc gtggctggcc gtggtgattc gcgtcgtcac catgaattcg
     9601 gcgctcgggt agaacaccgc catgaagcgg cgcaccacca ggtcgtacag cttttgctct
     9661 gcttccgaga gcaccccagg cgcttccagt gtggggatga tggcgaagtg gtccgacacc
     9721 ttgctgttgt cgaagatgcg cttgg
//
LOCUS       DAOMVO010000064         9722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1756_length_9722_cov_16.186718, whole genome shotgun sequence.
ACCESSION   DAOMVO010000064 DAOMVO010000000
VERSION     DAOMVO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_9722_cov_16.186718"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..655)
                     /locus_tag="PKV30_04890"
                     /pseudo
     CDS             complement(1..655)
                     /locus_tag="PKV30_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903032.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            complement(787..1776)
                     /gene="fmt"
                     /locus_tag="PKV30_04895"
     CDS             complement(787..1776)
                     /gene="fmt"
                     /locus_tag="PKV30_04895"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRL29590.1"
                     /translation="MSTKVAFAGTPEFARVALARLLAAGFDVPLVLTQPDRPAGRGMK
                     LQASPVKQLALEHGIAVAQPRSLRLDGKYPDDATAARTALLAAAPDVLVVAAYGLILP
                     QWVLDLPRLGCLNIHASILPRWRGAAPIHRAIEAGDAETGVTIMQMDAGLDTGDMLLL
                     EKLPIAPRASTATLHDQLAALGGRLIVEALELAACGGLHAKPQPAEGVTYAHKIEKHE
                     AAIDWSQPAAVIERRIRAFDPFPGASSTLGADVIKVWNSEIDSCSRLSDVRPGQILST
                     NESGIVVACADGALRLTELQRPGGKRLAARDFLRGFPLQPGQRLGTETPGDAP"
     gene            complement(1837..2346)
                     /locus_tag="PKV30_04900"
     CDS             complement(1837..2346)
                     /locus_tag="PKV30_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29591.1"
                     /translation="MTRGAGGWLAGALAAALLSGCAVFADPGRVAPGTPAAGVVEQLG
                     RPTARYPAAGNAGERLQYTYQPAGQRVFNIDLDAQGRVARVEQALSESLFAQRIQPNV
                     WTRADVLREYGPPARTMGVHNFDGTIWIWRYADGPTWRLLFIDIDRAGVVRGWSNGDE
                     DLPDPPDVR"
     gene            complement(2343..2873)
                     /gene="def"
                     /locus_tag="PKV30_04905"
     CDS             complement(2343..2873)
                     /gene="def"
                     /locus_tag="PKV30_04905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRL29592.1"
                     /translation="MALLPIIRYPDARLHKVAQPVATVDAALRQLIDDMFETMYEAKG
                     IGLAATQVDVHQRLIVIDVSEERNERLVLINPELLWASDERVKGDEGCLSVPGIYDGV
                     ERAERVKVRALDAQGTAREIEADGLLAVCIQHEMDHLLGKVFVEYLSPLKQGRIKTKL
                     QKAERQDKLDKAKEPA"
     gene            3017..4240
                     /locus_tag="PKV30_04910"
     CDS             3017..4240
                     /locus_tag="PKV30_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRL29593.1"
                     /translation="MKHTFPGATRALTAPLGLLAAALLAGQPASAQPVTPTQRGVAQQ
                     VASQGVPLAALAPNAPEEYTVRKGDTLWRISGMFLKSPWRWPELWGMNLKDIRNPHLI
                     YPGQHLYLERVGDRAFLRTGRADGQPGATIRVSPRNRVEMLGTGPLPTLEPHLIEPFL
                     TEPLVVDDDTFQRAPRIVALANQSRVLVAKGDRAYVRGPVDAPLLAAPGLPTDFRVFR
                     TATPLKDPISGEILGYEGQYVGQAALVRGESETEETVKAGFGGGTTQKVLPVPATIDV
                     TSSKEEMKAGDRLLPEPPREFRNYVPHVPDLPIEARVVSVHGNAVRYAGQNQIVVINK
                     GLRDGVENGQVLAILSTGPQMKDKTDKSRDTIKLPDERNGIAMVFRPFERVSYVLVME
                     IINPVQVGDKLINPN"
     gene            4272..5495
                     /gene="dprA"
                     /locus_tag="PKV30_04915"
     CDS             4272..5495
                     /gene="dprA"
                     /locus_tag="PKV30_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRL29594.1"
                     /translation="MDRPELEAWLRLTLTPGVGSDSARRLMAAFGLPPHVFEQTSAAL
                     REVVGPAPASALLKAPDTLATQLQATQDWLGAASDDGAPRHIVTLGDAAYPPSLLAIE
                     DPPCLLYVVGRLPGRDSGSKQAPALAESAPAASEFIANDWPQRALAMVGSRNPTPQGA
                     QNARQFARALAGGGVTIVSGLALGIDAAAHEGALDGSAPGALATIAVIGTGIDRVYPR
                     QHRDLAHRIAGRGLIVSEYPIGTPPLAHNFPRRNRLIAGLSQGTLVVEAALQSGSLIT
                     ARLASEQGKEVFAIPGSIHSPQSRGCHALIRQGAKLVETAEDVLEELRWPGNVVVTTK
                     SEAAGADPVSASGPKDPDHDLLEALGSDPVSLDALQARTGLDTPTLQARLLTLELDGR
                     VGRLPGGLFQRLALS"
     gene            5530..6033
                     /locus_tag="PKV30_04920"
     CDS             5530..6033
                     /locus_tag="PKV30_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HRL29595.1"
                     /translation="MYQVLVYVYENYGGGADTPDRQRLGMRLSSAGFERDEIQQALHW
                     LDGLDNVADGICLTAPNDAAAPLDTGSPIWPAARDSLRVYSPQEIEHLGPQGIACLRF
                     LEDAGALSAELRELVIDRALAASEAPLDLDDLKIIIMMVYWRMGANPDLLVLDELSDD
                     TRHRLAH"
     gene            complement(6083..8497)
                     /locus_tag="PKV30_04925"
     CDS             complement(6083..8497)
                     /locus_tag="PKV30_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HRL29596.1"
                     /translation="MVTSSTPPASEHRISRQVREQFVDALDNLIVELMGRIQAHLLQL
                     AQGGPRGAALSEMQLAMEASARFQRERQAWVAAVRQGWREALVAMRGGGELAFDPSAA
                     LGLVDDEVVERKIVASRIGSAVLDAAGAEFNNLRLRIQYLERTTDLAARDVLRPDVFA
                     QQLIKAWTGCGLTRAMWALVHAPVQADVVARMTAAFKQANATLIEQGVMAEIDLKSMV
                     RRTDGSSGPAAPRGAATAPGGPAEGAWRPSARAGSDARAAPPATRLDAPMTSGSMYAG
                     PAGIPDVQASALARAAQETRMMTAVTPMARVRQRAQGVLGQLKRLINERVADFDGTAP
                     VGPPSPQLARALSESVTPFAVTELIDSRGGGPTAALPIANVEAVATRLRQRATELKAK
                     ADKPSEKAIIEIVALMFQAILAEERMPAAIRVWFARLQIPVLRLALAEPDFFASIEHP
                     ARQLIDRMGACALGFDAATITGSRLEREIKRIVQVIEQYPETGRRVYQLVLDEFKKFL
                     GRSLTEGGKAQQMATLAQQVEQKEALAIQYTIELRRMLASVPVPEDVREFLFRVWAEV
                     LAMAAVRRGPQNEETLRYKQAAADLLWAVSPKSDRAERSRVVQHMAGLLQALRSGMGT
                     LALEADEQDAHVKRINDAVMQAFVSRGDGVSAERLAELSRSLAGLEDVVTDDAEGDVL
                     LDPGMIELMFGVEGDALEVVAEGGSQPSDGMLRWAHELELGAWFALDYLGQVGQVQYV
                     WCSRRGQLHLFSSGVGKSFLVQTRRMASYLQAGLLVPVEDEALTVRATREALVKLHAE
                     PERMLH"
     gene            complement(8563..9516)
                     /gene="secF"
                     /locus_tag="PKV30_04930"
     CDS             complement(8563..9516)
                     /gene="secF"
                     /locus_tag="PKV30_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRL29597.1"
                     /translation="MEFFKIGRDIPFMRYALIFNAISVATFVLALFFLITRGLHLSVE
                     FTGGTVMEVSYSQPADLTQVRGTVASLGYSDVQVQNFGTARDVLIRLPVQQGKTTAEQ
                     SQQVLQALKTATPDVELRRTEFVGPQVGQELAADGLKALAFVVIGIMIYLSMRFEWKY
                     AVAGIIANLHDVVIILGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVIFDRIRENFRR
                     FRKMSTVEVINNSITANISRTIITHGSTQIMVLSMLLFGGETLHYFAVALTIGILFGI
                     YSSVFVAAAIAMWLGIRREDLVKGGGRKEGDPNDPNAGAVV"
     gene            complement(9538..>9722)
                     /locus_tag="PKV30_04935"
     CDS             complement(9538..>9722)
                     /locus_tag="PKV30_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29598.1"
                     /translation="RGFAVVHCIGILTSMFSAVFFSRGLVNWWYGRKKKLKTVSIGTV
                     WRPENEAKLIDSGNAR"
BASE COUNT         1588 a         3357 c         3259 g         1518 t
ORIGIN      
        1 tgggcagctt cggctcgatg aacaggcaca gcgccaccgc caccacgatg gcgacgacga
       61 tgttcaggcg gtagggcagg ccgaacagcg ccacggcggc ggtggcggcg atcagggccg
      121 agaaggcgcg caggcgcgtg gcggccagcg agcaggtgac ggccagcagc gccagcgtgc
      181 cggcaaattc cagcccccag cgctcaggga acgccgcgcc catgaagatg cccagcatgc
      241 tgaaggtctg ccagaagatc cagttgcaca cgttgccgcc ccacaggtag gccatttgcg
      301 cgcggcgctg gcgctcggtc tcggccggcc tgggaaagcg acgggtgtac agcacgtatg
      361 tcacgtcgcc ggtgaagtag ccaagctaca agcgcctgat tcgcggcaga aacatgaagt
      421 attggcgcag atgcaggctg aagacgacaa atcgcaggtt gacgcaaaaa gccgccgccc
      481 agatgaccca cagcggcgcg ctggcggcaa tgagcggaat caccgccagc tgcgcgctgc
      541 cggcaaacac cagcagcgac atcagcaccg cttcggtcag gctcatgccg cttttgacca
      601 tggcgacgcc ggtcatgaac gaccatgccg tcaggccgac cgagatgccc gacatctcgc
      661 gcgcgccgac ccagaacagc gggtggcgcg cggtgtgggc caaggcttcg atgcggtcac
      721 ccacccattg gcccagccgc ccgggcgccg acaaaccgcc ctgcggatcg ggcggcagcg
      781 gcatgttcat ggtgcgtcgc ccggtgtctc ggtgcccagc cgctggccgg gctgcagcgg
      841 aaacccgcgc agaaagtcgc gcgcggccag gcgcttgccg ccggggcgct gcagctcggt
      901 caggcgcagg gcgccatcgg cgcaggcgac cacgataccg ctctcgttgg tggacaaaat
      961 ctggcccggg cgcacgtctg acaagcgcga gcagctatcg atttcggagt tccagacctt
     1021 gatgacatcg gcgcccagcg tgctgctggc gccggggaag gggtcgaagg cgcggatgcg
     1081 gcgctcgatc acggccgccg gctgcgacca gtcgatggcg gcttcgtgct tttcgatctt
     1141 gtgtgcgtag gtcacgccct cggccggttg cggcttggcg tgcaggccgc cgcaggccgc
     1201 cagctccagc gcctcgacga tcaggcggcc gcccagcgcg gccagttggt cgtgcagcgt
     1261 ggcggtgctg gcgcgcgggg cgatgggcag cttttcaagc agcagcatgt cgcccgtgtc
     1321 caggccggcg tccatctgca tgatggtgac gccggtctcg gcatcgcccg cctcgatggc
     1381 gcgatgaatc ggcgccgcgc cgcgccaccg cggcaggatg gaggcgtgga tgttcaaaca
     1441 ccccaggcgc ggcagatcga gcacccactg gggcaggatc agaccgtaag cggcgaccac
     1501 cagcacgtcg ggcgcggcgg ccagcaaggc ggtgcgcgca gcggtggcgt catcggggta
     1561 tttgccatcg agccgcaggc tgcgcggctg ggcgacggcg atgccgtgtt ccagcgccag
     1621 ttgcttgacc ggcgaggcct gcagcttcat gccgcgaccg gccgggcggt cgggctgggt
     1681 cagcaccagc ggcacgtcga aacccgccgc cagcagccgc gccagcgcca cgcgggcaaa
     1741 ttcgggggtg ccggcgaagg cgactttcgt gctcattttt tggtagctgc ttgcgcttgt
     1801 cagtcaagcg ctggaggccg atttggcttg aggtgctcag cgcacgtcgg gtgggtcggg
     1861 caagtcttca tcaccgttcg accagccgcg caccacgcca gcgcggtcga tgtcgatgaa
     1921 cagcaggcgc caggtggggc cgtccgcgta gcgccagatc cagatcgtgc cgtcgaagtt
     1981 gtgcacgccc atggtgcgcg cgggcgggcc gtattcgcgc agcacgtcgg cgcgcgtcca
     2041 cacattgggc tgaatgcgct gggcaaacag gctttcgctg agcgcctgct cgacgcgcgc
     2101 cacgcggcct tgcgcgtcca ggtcgatgtt gaacacgcgc tggccggccg gttggtaggt
     2161 gtattgcagg cgttcgcccg cgttgccggc ggccgggtag cgcgcggtgg ggcggcccag
     2221 ttgctcgacc acgcccgccg ccggcgtgcc gggcgccacg cggccggggt cggcaaacac
     2281 ggcgcagccg ctgagcagcg cggccgccag tgcgccggcc agccagccgc cggcgccgcg
     2341 cgtcatgccg gctccttggc cttgtccagc ttgtcttgcc gctctgcttt ttgcagcttg
     2401 gtcttgatgc ggccctgttt gagcggcgac aggtattcga cgaacacctt gcccagcagg
     2461 tggtccatct cgtgctggat gcagacggcc agcaggccat cggcctcgat ctcgcgcgcc
     2521 gtgccctggg catcgagcgc gcgcaccttc acccgctcgg cgcgctcgac gccgtcgtag
     2581 atgccgggca ccgacaggca gccttcgtcg cctttcacgc gctcgtcgct ggcccacagc
     2641 agctcggggt tgatcagcac caggcgctcg ttgcgttctt ccgacacgtc gatcacgatc
     2701 aggcgctggt gcacgtcgac ctgggtggcg gccaggccga tgcccttggc ctcgtacatg
     2761 gtctcgaaca tgtcgtcgat cagctggcgc agggcggcat cgaccgtggc gacgggttgg
     2821 gccaccttgt gcaggcgggc gtcggggtag cgaataatcg gtagcagggc catcggacgg
     2881 gttccacaga tgtcgctatt ttcgctactt ttgacgtttc gcgccgtcgg tcgggggcat
     2941 tttcgttgcc ggatcaaggg cttgactgca gaatgtccag tgctttgtca agttggcagc
     3001 cgccctcagc aaaacaatga aacatacttt tcctggcgcc acgcgcgccc tgaccgcgcc
     3061 cctcggcctg ctggcggccg ccctgctggc cggccaaccg gccagcgcgc aacccgtcac
     3121 ccccacgcag cgcggcgtcg cgcaacaagt ggccagccag ggcgtgccgc tggcggcgct
     3181 ggcgcccaac gcgcctgagg aatacacggt gcgcaagggc gacacgttgt ggcgcatctc
     3241 cggcatgttc ctcaaatcgc cctggcgctg gcccgagctg tggggcatga acctcaagga
     3301 catccgcaac ccgcacctga tctaccccgg ccagcacctg tacctggagc gcgtgggcga
     3361 ccgcgccttc ctgcgcaccg ggcgtgccga cggccagccg ggcgccacca tccgcgtgtc
     3421 gccgcgcaac cgcgtcgaga tgctgggcac cggcccgctg ccgacgctgg agccgcacct
     3481 gatcgagccc ttcttgaccg agccgctggt ggtcgatgac gacaccttcc agcgcgcgcc
     3541 gcgcatcgtg gcgctggcca accagagccg cgtgctggtg gccaagggcg accgcgctta
     3601 cgtgcgcggc ccggtcgatg cgccgctgct ggcggcgccg ggtttgccga ccgatttccg
     3661 cgtcttccgc accgccacgc cgctgaaaga cccgatctcc ggcgaaatcc tgggctacga
     3721 aggccagtac gtcggccagg ccgcgctggt gcgcggcgaa agcgagaccg aagaaaccgt
     3781 caaggccggc tttggcggtg gcaccacgca gaaggtgctg ccggtgccgg ccaccatcga
     3841 cgtcaccagc agcaaggaag aaatgaaggc gggcgaccgc ctgctgcccg agccgccgcg
     3901 cgagttccgc aactacgtgc cgcacgtgcc cgatctgccg attgaggcgc gcgtggtgtc
     3961 ggtgcatggc aacgcggtgc gctatgccgg ccagaatcag atcgtggtga tcaacaaggg
     4021 gctgcgcgac ggcgtcgaga acggccaggt gctggcgatt ctgtcgaccg gcccgcagat
     4081 gaaggacaag accgataagt cgcgcgacac catcaagctg cccgacgaac gcaacggcat
     4141 tgccatggtg ttccgcccgt tcgagcgcgt gtcgtacgtg ctggtgatgg aaatcatcaa
     4201 cccggtgcag gtgggcgaca agctcatcaa ccccaactga agcgcttgcg atccgcgccg
     4261 gcggtgatgc catggatcgc cccgaactcg aagcctggct gcgcctgacg ctgacgccgg
     4321 gcgtcggcag cgattcggcg cggcgcctta tggcggcgtt tggtctgccg ccgcacgtct
     4381 tcgaacaaac cagcgccgcg ctgcgcgagg tggtgggccc tgcgccggcc agcgcgctgc
     4441 tgaaggcgcc ggatacgctg gcgacgcagc tgcaagccac gcaggactgg ctgggcgccg
     4501 cctctgacga cggcgcgccg cgccacatcg tgacgctggg cgacgcggcc tatccgccaa
     4561 gcctgctggc gatcgaggac ccgccctgcc tgctctacgt cgtgggtcgg ctgccgggcc
     4621 gagattcagg gtccaaacag gcgccagcgc ttgccgaatc agcgccagca gcttctgaat
     4681 tcatagcgaa cgactggcca caacgcgccc tggccatggt tggcagccgc aacccgacgc
     4741 cgcagggcgc acagaacgcg cgccagttcg cgcgtgcctt ggccggcggc ggcgtgacca
     4801 tcgtgtccgg gctggcgctg ggcatcgatg cggcggcgca cgaaggcgcg ctggacggct
     4861 ctgcgccagg cgcgctggcg accatcgccg tcatcggcac cggcatcgat cgcgtgtacc
     4921 cgcgccagca ccgcgatctg gcgcaccgca tcgccgggcg cggcttgatc gtgagcgaat
     4981 acccgatcgg cacaccgccg ctggcgcaca actttccgcg ccgcaaccgg ctgatcgccg
     5041 ggctgagcca gggcacgctg gtggtcgagg cggcgctgca gtcgggctcg ctcatcaccg
     5101 cgcggctggc cagcgagcag ggcaaagagg tgtttgccat tcctggctcc atccacagcc
     5161 cgcagtcgcg cggctgccac gcgctgatcc gccagggcgc caagctggtt gagacggccg
     5221 aggacgtgct ggaggagctg cgctggccgg gcaacgtcgt cgttactaca aaaagcgaag
     5281 ctgccggcgc tgatccagta agcgccagcg gcccaaaaga cccggatcac gacttgctcg
     5341 aagcgctggg ctccgacccg gtcagcctcg acgcgctgca ggcgcgcacc ggcctggaca
     5401 cgcccacgct gcaggcgcgt ttgctgacgc tggaactcga cggccgcgtc ggccgcctgc
     5461 cgggcggctt gttccagcgt ctggccttga gctgaaggcg gcgcgccacc ccggtgctat
     5521 agtgaccgca tgtaccaagt gctggtgtac gtgtacgaaa actacggcgg cggcgccgat
     5581 acccccgacc gccagcggct tggcatgcgc ttgtccagcg ccggcttcga gcgcgacgag
     5641 atccagcagg cgctgcactg gctggatggc ctggacaacg tggccgacgg aatctgcctg
     5701 accgccccga acgatgccgc cgcgccgctc gatacgggca gcccgatctg gcccgcagcc
     5761 cgggacagcc tgcgcgtcta cagcccgcag gaaatcgagc acctggggcc ccaaggcatc
     5821 gcctgcctca ggtttctgga agacgctggc gcgctgtcgg ccgagttgcg tgaattggtg
     5881 atcgaccgcg cgctggcggc atcggaagcg ccgctcgatc tggacgatct gaagatcatc
     5941 atcatgatgg tgtactggcg catgggcgcc aacccggact tgctggtgct cgacgagctg
     6001 tccgacgaca cccggcaccg gctcgcgcat tgagcatcaa acagcggcct ggcgcttgtc
     6061 cagcctgcgc cagcagctcc gattaatgta gcatccgttc cggctcggca tggagcttga
     6121 ccagcgcctc gcgcgtggcg cgcacggtga gcgcctcgtc ttccaccggc accagcaagc
     6181 cagcctgcag gtacgaggcc atgcggcgcg tctgcaccag aaagctcttg ccgacgcccg
     6241 acgagaacag gtgcaactgc ccgcgccggc tgcaccacac gtactgcacc tggccgacct
     6301 ggcccaggta gtcgagcgcg aaccaggcgc ccagctccag ctcgtgcgcc cagcgcagca
     6361 tgccgtcgct gggctgcgat ccgccttcag ccaccacctc aagcgcatcg ccctcgacgc
     6421 cgaacatcag ctcgatcatg ccgggatcga gcagcacgtc gccctcggcg tcgtcggtca
     6481 ccacgtcttc cagcccggcc aggctgcgcg acagctcggc cagccgctcg gccgacacgc
     6541 catcgccgcg cgacacaaag gcctgcatca cggcgtcgtt gatgcgcttg acgtgggcgt
     6601 cctgctcatc ggcctccagc gccagcgtgc ccatgccgct gcgcagcgcc tgcagcaggc
     6661 ccgccatgtg ctgcaccacg cggctgcgct cggcgcggtc ggacttggga ctcacggccc
     6721 acagcaggtc ggccgccgcc tgcttgtagc gcagcgtttc ttcattctgc ggaccgcgcc
     6781 gcaccgccgc catggccagc acctcggccc agacgcggaa caggaattca cgcacgtctt
     6841 ccggcaccgg caccgacgcc agcatgcgac gcagctcgat ggtgtactga atcgccagcg
     6901 cctccttctg ctcgacctgc tgcgccagcg tcgccatctg ctgcgccttg ccgccttcgg
     6961 tgagcgagcg gccaagaaac ttcttgaact cgtccagcac cagctggtag acgcggcggc
     7021 cggtctcggg atactgctcg atcacctgca cgatgcgctt gatctcgcgc tccagccggc
     7081 tgccggtgat ggtggcggcg tcgaagccga gtgcgcacgc gcccatgcgg tcgatcagct
     7141 ggcgcgcggg gtgctcgatg gaggcaaaga aatccggctc ggccagcgcc agccgcagca
     7201 ccggaatctg caggcgcgca aaccagaccc ggatggcggc cggcatgcgc tcttcggcca
     7261 ggatggcctg gaacatcagc gccacgatct cgatgatggc cttctcgctc ggcttgtcgg
     7321 ccttggcctt gagttcggtc gcgcgctggc gcaggcgcgt ggccaccgcc tcgacgttgg
     7381 caatcggcag cgcggcggtg gggccaccgc cgcgtgagtc gatcagttcg gtgacagcga
     7441 agggcgtgac cgattcggac agcgcgcgcg ccagttgcgg cgacggcggc ccgaccggtg
     7501 cggtgccgtc gaaatcggcc acgcgctcgt tgatcaagcg cttcagctgg cccagcacgc
     7561 cttgcgcgcg ctggcgcacg cgcgccatcg gcgtgaccgc cgtcatcatg cgcgtttcct
     7621 gcgccgcgcg ggccagggcc gaagcctgta cgtcgggtat gccggccggg cccgcgtaca
     7681 tcgatccgct ggtcatgggc gcgtccagcc gcgtggcagg cggggcagcg cgggcgtcgc
     7741 tgccggcgcg cgcggacggg cgccaggcgc cttcggccgg cccgccaggg gcggtcgcgg
     7801 cgccgcgtgg ggccgctggc ccgctgctgc catcggtgcg ccgcaccatc gatttcagat
     7861 cgatttcggc catcacgcct tgttcgatca gcgtggcgtt cgcctgtttg aacgccgccg
     7921 tcatgcgcgc taccacatcg gcctgcacag gcgcatgcac cagcgcccac atggcgcgcg
     7981 tcaggccaca gcctgtccag gccttgatca gctgctgggc aaatacgtcc gggcgcagca
     8041 catcgcgcgc cgccaagtcg gtggtgcgct ccaggtattg aatgcgcaaa cgcaggttgt
     8101 tgaattcggc acccgccgca tccagcaccg ccgagccgat gcgcgaggcg acgatcttgc
     8161 gctcaacgac ttcgtcgtcg accaggccca gggctgcgga gggatcaaat gccagttcgc
     8221 cgcccccgcg catggcgacc aacgcttcac gccacccctg acgtaccgca gctacccagg
     8281 cttgccgctc acgttgaaac cgtgcactgg cttccatcgc caactgcatt tccgacagcg
     8341 ctgcgccgcg tgggccgcct tgggccaact gaagcaagtg cgcttgaatg cgccccatca
     8401 actcgacaat caagttgtcc agcgcatcaa cgaactgctc tcgcacttga cgcgagatgc
     8461 ggtgttccga tgcagggggc gtggacgacg tgaccatgag cgcgcagggg ccgctcagta
     8521 tcgcatgacg agcggctcag cccagcgtgt tttcccgctt cgtcagacga ccgcgccggc
     8581 gttcggatcg ttcggatcgc cttctttgcg accgccgccc ttgaccagat cttcgcgccg
     8641 gatacccagc cacatggcaa tcgccgcggc cacgaacacc gacgagtaga tgccgaacag
     8701 gatgccgatg gtgagcgcaa cggcgaagta gtgcagcgtc tcgccgccga acagcagcat
     8761 cgacagcacc atgatctgcg tcgagccgtg cgtgatgatg gtgcggctga tgttggcggt
     8821 gatggagttg ttgatcacct ccacggtgct catcttgcgg aagcgccgga agttctcgcg
     8881 gatgcggtcg aagatgacca cggattcgtt caccgaatag cccagcaccg ccagcaccgc
     8941 cgccagcacc gagagcgaaa actcccactg gaagaaggcg aaaaagccca ggatgatcac
     9001 cacgtcgtgc aggttggcga tgatgccggc caccgcgtac ttccactcga agcgcatcga
     9061 caggtagatc atgatgccga tcaccacgaa ggccagcgcc ttcaggccgt cggccgccag
     9121 ttcctgcccg acctgcggac cgacgaattc ggtgcgccgc agctcgacat cgggtgtcgc
     9181 ggtcttgagc gcctgcagca cttgctgact ctgctcggcc gtggtcttgc cctgctgcac
     9241 cggcaggcga atcagcacat cgcgcgcggt gccgaagttc tgcacctgca cgtcgctgta
     9301 gcccagcgac gccacggtgc cgcgcacctg ggtcaaatcg gccggctggc tgtagctcac
     9361 ttccatcacg gtgccgccgg tgaactcgac cgacaggtgc agcccgcgcg tgatcaggaa
     9421 gaacagcgcc agcacgaagg tggcgaccga tatggcgttg aagatcaacg cataccgcat
     9481 gaagggaatg tcccgaccga tcttgaagaa ttccatgttc agttttcctt cgcgggctca
     9541 gcgggcgttg ccgctgtcaa tcagcttggc ctcgttttca ggccgccaca cggtgccgat
     9601 agacacggtt ttcagctttt tcttccgccc ataccaccag ttgaccaggc cgcgcgagaa
     9661 gaacacggcc gagaacatcg aggtcaggat gccgatgcag tgcaccaccg cgaagccgcg
     9721 ca
//
LOCUS       DAOMVO010000065         9439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1830_length_9439_cov_12.496057, whole genome shotgun sequence.
ACCESSION   DAOMVO010000065 DAOMVO010000000
VERSION     DAOMVO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1830_length_9439_cov_12.496057"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..2118)
                     /locus_tag="PKV30_04940"
     CDS             complement(232..2118)
                     /locus_tag="PKV30_04940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRL29599.1"
                     /translation="MERAEFMHLLRLSEQASQEDSARYRRGVWLFAALGYAWVIGCLL
                     LGAAILGGIAWSVTHGGRFRGVYVWAVLGAGALLWVSLRALWLRLEPPTGHELKRDDA
                     RALFDLLERIRAKIKGPPIHRVLIDDDYNASIQQHPRFGLFGGAINTLTIGLPLLMAL
                     DTRRLAAVLAHEYGHLRGDHGRFAAWIYRSRLSWAKLHDGLAHDSGVASWATNAFLNW
                     YCPRFSAKTFALARQDEYEADRIAGRIAGTDVAAAALTEVAVKGAWLREQFWPAHWRG
                     AGAQPLPVGPFSAMRKLLAQPLDTAFAQDALRAELKALPEVDDTHPGLRDRLEALDAA
                     KTLPDWSAKPAMGLLADRRRWVAQFDSAWCKANASAWKQHHANLARVRQRMAYLKARA
                     GSANAAEWTEMGELTHRLDPTAPAAALFERALAATPGHPAALRGVIAEMPPADARRMP
                     LIEQLHDASAPHRWWSARQAVATLERAVAEGRGSEADLKRWRERLKQADDAERRAWEE
                     LAEPPHLQGIARHDLNEFELGELQGALARFGHVQTAWLVSKRLKEFAWRRAYLLLVEL
                     PRHDDETRYAMCRHLEQLLHLPGPVLALWAGHDPTLQDIQRQCFEPVYVAAAPVVRAG
                     SASR"
     gene            complement(2276..2857)
                     /gene="purN"
                     /locus_tag="PKV30_04945"
     CDS             complement(2276..2857)
                     /gene="purN"
                     /locus_tag="PKV30_04945"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRL29600.1"
                     /translation="MKSIVILISGTGSNMAAIVRACERERWAERLNARVAAVISNRPG
                     AKGLDFAREQGIAAAVVDHKAFDGREAFDAALAAEIDRHDPALVVLAGFMRILTPGFV
                     AHYAGRLINIHPSLLPAFPGLHTHQRAIDEGCKLAGATVHQVTAELDHGHILAQAAVP
                     VLPGDTAERLAARVLTQEHLIYPRAVAELIQKL"
     gene            complement(2996..3430)
                     /locus_tag="PKV30_04950"
     CDS             complement(2996..3430)
                     /locus_tag="PKV30_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29601.1"
                     /translation="MSLTGAQVQDVLPAPLVIDGTITRSCTGGTLTGAVGGSTLALSG
                     TTIPTGGCSVTVPVRWPYAAGAASCPAGAPTTATNTITPPAQFSTDQGQSTTPATATL
                     QCDPTLAPVVPPAVAAVPTLHGALLALLGAALAVFGWRQRPA"
     gene            complement(3447..4349)
                     /locus_tag="PKV30_04955"
     CDS             complement(3447..4349)
                     /locus_tag="PKV30_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29602.1"
                     /translation="MKFWNKVARLVRAAGVTSLLAIGAAGLGLSSAAHAQKVLILQTT
                     ETGTGDDTGAANIHLNMLAEFENAGASVTVQNGLQTAGTVDAATFNATPPYDLVVVSS
                     VYQAPGAGNMSAIQAAVSARSANAFVFFMDGYVDSSVTQLASLLSAVSGNAISGAVTL
                     VGVQYFPLNAASPNQGSFFGMPTIRGGIARYLDGVPAGNMLYLPPVGDPAAQSAYAAF
                     FPQTQVAGGNGLARWRFRYDAVLQRRSFPVLRVDSGQDGACAVRRIAGPRRRMRPAGG
                     QCDQIVRSSHGGAGWRRHTDNPAQ"
     gene            4473..5180
                     /locus_tag="PKV30_04960"
     CDS             4473..5180
                     /locus_tag="PKV30_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HRL29603.1"
                     /translation="MSLRPLTPIEARVLATLMEKARTVPDTYPLSLNGVVTGCNQKTS
                     REPVMNVGEGQAQEALDDLRALSLVNESHGSRVAKYEHNFQRVYGVPEQSAVLLGLLM
                     LRGPQTAGELRINTERWYRFADISSVEGFLHELAERADDKGGPLVLKLPRTPGTREDR
                     WAHLICGPVEFSPESIAAGASGASAGGQNDRISALEAEVAALRATVERLCAELGVSPA
                     QPDGGGAHAKNENSLET"
     gene            5184..6392
                     /locus_tag="PKV30_04965"
     CDS             5184..6392
                     /locus_tag="PKV30_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL29604.1"
                     /translation="MDLAFTPEELKFRDEIRDWVKGALPKAISDKVHQAQRLTRDDMQ
                     TWAKILGKKGWLGYGWPAQFGGPGWTAVQKHLFEEELAMAGAPRIVPFGPVMVAPVIM
                     AYGNKEQQERFLPGIASGEVWWSQGYSEPGSGSDLASVRTKAEKKGDKYIVNGQKTWT
                     TLGQYGEWMFNLVRTSNEGKPQTGISFLLIDMKSPGVSVRPIKLLDGEVEVNDVFFDN
                     VEVPVENLIGEENKGWTYAKHLLSHERTNIADVNRAKRELERLKRIAKAEGVYDDQRF
                     RDQIALLEIDVVALEMLVLRVLSAEKSGKNSLDIAGLLKIKGSEIQQRYTELMMLAAG
                     PYSLPFIEEAMNAGWQGDQAAAAGLQGFPGGDVANATLAPSYFNYRKTTIYGGSNEVQ
                     RNIVAQTVLG"
     gene            6408..7523
                     /locus_tag="PKV30_04970"
     CDS             6408..7523
                     /locus_tag="PKV30_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL29605.1"
                     /translation="MDFDFSDDQQALREAVAKWVEKSYDFERRQQIVKSGGFDRAVYN
                     ELAELGLTGIYVSEEYGGMGMGPVEAMVVLEELGRGIVTEPLAQAFVTSAVIQAYGSD
                     GVKQQWLPKIASGEALVVLAQQERKARHQLDVCEAKAAKSGDSYALTALKNIVPAGDQ
                     ADAFVVPAMLDGKLALFIVERAAKGVSTRGYLTQAESRAADVKLDNAPAQLITTDGLT
                     ALNFAVDVGIACVCAEGVGAMDQTLKLTTEYMNQRKQFGVTIATFQALRHRVADMKMQ
                     LELARSMSYYATLKLGAPEAERRQALSRAKVQLGNSMRFVGQQSVQLHGGIGVTDEYA
                     GSHYFKVLTQLEMTYGDTLHHLGEVSQRMTETAGVFA"
     gene            complement(7570..9129)
                     /gene="garD"
                     /locus_tag="PKV30_04975"
     CDS             complement(7570..9129)
                     /gene="garD"
                     /locus_tag="PKV30_04975"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HRL29606.1"
                     /translation="MTTPRPPLAIRLHAADNVAIVANDGGLPSGTQLPDGLTLRDKVP
                     QGHKVALTDIEAGGVVRRYNVPVGYANRAIAAGSWVPERLLDIPAARVLDGLPMATVP
                     APAWAPLQGYTFEGYVNADGSVGTRNLLAITTTVQCVAGVVGIAAERIRTELLPLFPN
                     VDGVVALEHTYGCGVAIDAPGAEIPQRTLRHISQNPNFGGELLMVSLGCEKLQPDRLI
                     PPGTIGLRPEGAALNNVCLQDDSHVGFMSMIDDIVQRARQILERLNARRRQTVPAAKL
                     VVGVQCGGSDAFSGVTANPAVGVMADLVVRAGGTLMFSENTEVRDAVDQLTSRAATPA
                     VAEAIVRELGWYDQYLDRGRVDRAANTTPGNKAGGLSNITEKAMGSVIKSGSAAIASV
                     LPPGEKLKADQTGLVFAATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAAVPVVKV
                     ATRSDLARRWHDLMDVNAGRIADGEQTIEQAGLEMFELLLDVASGRQKTWAERWKLHN
                     ALVLFNPAPVT"
     gene            complement(9291..>9439)
                     /locus_tag="PKV30_04980"
     CDS             complement(9291..>9439)
                     /locus_tag="PKV30_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRL29607.1"
                     /translation="FFALDNVVLVPHIASATVQTRQAMAQRVLDNLDAFVAGQPMPSA
                     VQAP"
BASE COUNT         1603 a         3143 c         3224 g         1469 t
ORIGIN      
        1 tcagcctaat gataaaaacg gccgccagcg cttgactggc gagcgcgagc agctatggtt
       61 tttgatgatc acgcctgcgg cgcatgaccc tcggcttcgc cttcgatgac ggcgctgcgc
      121 gccatgacgg ggcgtccggt cattccgtca tggcgcgcag cgccgtcgtt tcgtcatcca
      181 aacaggccgc agcgcttgac tgaaaagcgc aagcagctac tttaataata gctatctgga
      241 tgcagagccc gcgcgcacga caggcgcagc cgccacatac accggctcaa agcactggcg
      301 ctgaatgtcc tgcagcgttg gatcgtgccc ggcccacagc gccagcacgg ggccgggcag
      361 gtggagcagt tgctccaggt gacggcacat cgcatagcgc gtttcgtcgt cgtggcgtgg
      421 cagctcgacc agcagcaggt aagcgcgccg ccaggcaaat tccttcagcc gcttgctgac
      481 gagccacgcg gtctgcacgt ggccaaagcg cgccagcgcg ccctgcagct cgcccagctc
      541 gaactcgttc aggtcgtggc gcgcaatgcc ttgcaggtgt ggcggctcgg ccagttcttc
      601 ccacgcgcgg cgctcggcgt catcggcctg cttcaggcgc tcgcgccagc gcttcaggtc
      661 ggcttcgctg ccgcgccctt cggcgaccgc gcgctccagc gtggcgacgg cctggcgcgc
      721 ggaccaccag cggtgcgggg cgctggcgtc gtgcagttgc tcgatcagcg gcatgcggcg
      781 cgcatcggct ggcggcatct cggcgatcac gccgcgcagc gcggcggggt ggccgggcgt
      841 ggccgccagc gcgcgctcaa acagcgcggc ggcgggcgcg gtggggtcga gccggtgcgt
      901 cagctcgccc atctcggtcc actcggcggc gttggcgctg ccggcgcggg ctttcaggta
      961 agccatccgc tgccgcacgc gggccaggtt ggcgtggtgc tgcttccagg cgctggcatt
     1021 ggccttgcac caggcgctgt cgaactgggc gacccagcgg cgccggtcgg ccagcaggcc
     1081 catggcgggc ttggcggacc agtcgggcag cgtctttgcg gcgtccagcg cttcgagccg
     1141 gtcgcgcaag ccggggtggg tgtcgtcgac ctccggcagt gctttcagct cggcgcgcag
     1201 cgcgtcttgc gcgaaggccg tgtccagcgg ctgcgccagc agtttgcgca tggcgctgaa
     1261 cggccccacg ggcagcggct gcgccccggc gccgcgccag tgggcgggcc agaactgctc
     1321 gcgcagccag gcgcctttca ccgccacttc ggtcagcgcg gcggcggcca catcggtgcc
     1381 ggctatgcgg ccggcgatgc ggtcggcctc gtattcgtcc tgccgcgcca gcgcaaaggt
     1441 cttggccgaa aagcgcgggc aataccagtt gaggaaggcg ttggtggccc aactggcgac
     1501 gccgctgtcg tgcgccagcc cgtcgtgcag cttggcccac gacaggcggc tgcggtaaat
     1561 ccaggcggca aagcggccgt ggtcgccgcg caggtggccg tattcgtgcg ccagcacggc
     1621 ggccaagcgg cgcgtgtcga gcgccatcaa aagcggcagg ccgatggtga gggtattgat
     1681 ggcgccgcca aacaggccga agcgcgggtg ctgctggatg ctggcgttgt agtcgtcgtc
     1741 gatcagcacg cggtggatcg gcgggccctt gatcttggcg cggatgcgct ccagcagatc
     1801 gaacagcgcg cgggcgtcgt cgcgcttcag ttcatggccc gtgggcggct ccagccgcag
     1861 ccacagcgcg cgcaggctga cccacagcag ggcgcccgcg cccaataccg cccaaacgta
     1921 gacgccgcga aagcgcccgc cgtgcgtcac gctccaggcg atgccgccca gaatcgccgc
     1981 gcccagcagc agacagccga tgacccaggc gtagcccagc gccgcgaaca accacacgcc
     2041 gcgccgatag cgcgcgctgt cttcctggct ggcctgctcc gacaggcgca gcagatgcat
     2101 gaattcggcg cgttccatgg cgccaccctt ccctcgttct gaatggatga gaattagaac
     2161 gaagttttga cgaagtgggc agcggcgcgg cgcgggcgcg gcgtttttgc aacgcctgcg
     2221 ggcacgtcaa tattgaagaa acgcgctctg gcacatgcca gacaagcgcg cgcagctaca
     2281 gtttttgaat caactcggcg acggcgcgcg gatagatcaa atgctcttgc gtcagcacgc
     2341 gggcagccag ccgctccgcc gtgtcgcccg gcagcaccgg caccgccgcc tgcgccagga
     2401 tgtggccgtg gtccagctcg gccgtcacct ggtgcaccgt ggcgccggcc agcttgcagc
     2461 cctcatcaat ggcgcgctgg tgcgtgtgca agcccgggaa ggccggcaac aggctggggt
     2521 ggatattgat gagccgcccc gcgtaatgcg ccacaaagcc gggcgtcagg atgcgcatga
     2581 agccggccag caccaccagc gccggatcgt gccggtcgat ctcggcggcc agcgcggcgt
     2641 cgaaggcttc gcggccgtcg aacgccttgt gatcgaccac cgctgcggcg atgccctgct
     2701 cgcgcgcaaa gtccagcccc ttggcgcccg gccggttgct gatcaccgcc gccacgcgcg
     2761 cattcagccg ctcagcccag cgctcgcgct cgcacgcacg cacgatggcg gccatgttgc
     2821 tgccggtgcc ggagatcagg atgacgatgg atttcatcgg cggcgattat cggcgcgccg
     2881 ggtgcggcgc ccgcgcccaa gcggctggcg cccgtcacga atcgcagtcg aaaatgatgc
     2941 tggcgcaggt tggacgcgcg tcagcagctc tcttaccaat agcagactgc tcgcgtcaag
     3001 cgggccgctg gcgccagccg aagacggcca gtgcggcgcc cagcaacgcc agcaacgcgc
     3061 cgtgcagcgt gggcaccgcg gcgacggcgg gcggcaccac gggcgccaat gtggggtcgc
     3121 actgcagcgt ggcggtggct ggcgtggtcg attggccctg gtcggtcgag aactgcgccg
     3181 gtggcgtgat ggtgttggta gctgtggtgg gcgcgccggc cgggcaactc gccgcgccgg
     3241 ccgcgtaggg ccagcgcact ggcacggtca cgctgcaacc accggtggga atggtggtgc
     3301 cagagagcgc caaggtgctg ccccccacgg cgcccgtcag cgtgccgcca gtgcagcttc
     3361 gcgtgatggt gccgtcgatg accagcggcg ccggcagcac gtcctgcacc tgcgcgccag
     3421 tcagggacat cgcggggttg ctcaggttat tgagccggat tgtcagtgtg gcgccgccac
     3481 ccggcgccac cgtggctgga gcgaacgatt tggtcacact ggccgccggc aggccgcata
     3541 cgccgccggg ggcctgcaat gcggcgaaca gcgcaggcgc cgtcttgccc cgaatcgacg
     3601 cgtagaactg gaaacgatct ccgctgtagt actgcgtcgt atcggaatcg ccaacgggcc
     3661 agcccattgc caccagcgac ctgggtttgc ggaaagaacg ccgcgtacgc cgattgggcg
     3721 gccggatcgc cgacaggcgg caggtacagc atattcccgg ccggcacacc gtccagatag
     3781 cgcgcgatgc ctcctcggat ggtgggcatg ccgaagaagc tgccctggtt aggcgacgcc
     3841 gcgttcaggg ggaaatactg aacgccgaca agggtgacgg cgcctgaaat ggcgtttccg
     3901 ctgactgcac tcaacaaact ggccaactgg gtcacgctgg agtccacgta gccgtccatg
     3961 aaaaaaacga aggcattggc cgagcgcgcg ctcaccgccg cctggatcgc gctcatgttg
     4021 ccggcaccgg gcgcttgata gaccgaggaa accaccacca agtcgtacgg cggtgttgcg
     4081 ttgaaggtcg ccgcgtccac cgtccccgct gtctgcaggc cgttctggac cgtcactgag
     4141 gcgcccgcat tttcgaactc cgcgagcatg ttcaggtgga tgttggccgc gcccgtgtcg
     4201 tcgcccgtgc ccgtttccgt ggtctgcagg atgagcacct tttgcgcgtg tgccgcgctc
     4261 gacaaaccga ggcctgcggc gcctatggcc agcagcgacg tgacgcctgc agcgcgcacc
     4321 agccgcgcaa ccttgttcca gaacttcatg acgactaccc cctgaatttt gatccgtgca
     4381 ggtgccggcg taacgggtgc cgagcgcgct gttcgcctcg ccgctgcgtg ctggcgccca
     4441 gtcggccggc aactttggct attcttcacc ccatgtctct gcgccccctc acccccatcg
     4501 aagcccgcgt gctggccacg ctgatggaaa aagcccgcac ggtgccggac acctaccccc
     4561 tgtcgctcaa cggggtcgtc accggctgca accagaagac cagtcgcgag ccggtgatga
     4621 acgtgggcga aggccaggcg caggaggcgc tggacgacct gcgcgccctg agtctggtca
     4681 atgaatcgca cggcagccga gtcgccaagt acgagcacaa ctttcagcgc gtctacggcg
     4741 tgcccgagca atcggcggtg ctgctgggct tgctgatgct gcgcgggccg cagacggcgg
     4801 gtgaattgcg catcaacacc gagcgctggt accgctttgc cgacatctcg tcggtcgaag
     4861 gctttctgca cgagctggcc gagcgcgccg acgacaaggg cggcccgctg gtgctcaagc
     4921 tgccgcgcac gcccggcacg cgcgaggacc gctgggcgca tctgatctgc gggccggttg
     4981 agtttagtcc tgaatcgata gctgctggcg caagtggggc aagcgctggc ggccaaaatg
     5041 accgcatcag cgcgctggaa gccgaggtcg ccgcgctgcg cgcgacggtg gagcgcctgt
     5101 gcgcggagct gggcgtgtcg cctgcgcaac cggatggcgg cggcgcccat gctaaaaatg
     5161 agaactcact ggagacttga ccgatggatc tggctttcac ccccgaagaa ctgaaattcc
     5221 gcgacgagat tcgcgactgg gtcaagggcg cgctgcccaa ggccatttcc gacaaagtcc
     5281 accaggctca gcgcctgaca cgcgacgaca tgcaaacctg ggccaagatc ctgggcaaga
     5341 agggctggct gggctacggc tggccggcgc agtttggcgg ccccggctgg accgccgtgc
     5401 aaaagcactt gtttgaagaa gagctggcca tggccggcgc gccgcgcatc gtgccgtttg
     5461 gcccggtgat ggtggcgccc gtcatcatgg cctacggcaa caaggaacag caagagcgct
     5521 ttctgcccgg catcgcctca ggcgaggtgt ggtggagcca gggctacagc gagccgggtt
     5581 cgggctctga cttagccagc gtgcgcacca aggccgagaa aaaaggcgac aagtacatcg
     5641 tcaacggcca gaagacctgg acgacgctgg gccaatacgg cgagtggatg ttcaacctgg
     5701 tgcgcaccag caacgaaggc aagccgcaga ccggcatctc cttcttgctg atcgacatga
     5761 agtcgcccgg cgtgagcgtg cgaccgatca agctgctgga tggcgaagtc gaggtgaacg
     5821 acgtgttctt cgacaacgtc gaggtgccgg tcgagaacct gatcggcgaa gaaaacaagg
     5881 gctggactta cgccaagcac ctgctgagcc acgagcgcac caacatcgcc gacgtgaacc
     5941 gcgccaagcg cgagttggag cgcttgaagc gcatcgccaa ggccgagggc gtgtacgacg
     6001 accagcgctt tcgcgaccag atcgcgctgc ttgagatcga cgtggtggcg ctggagatgc
     6061 tggtgctgcg cgtgctgagc gccgagaaat cgggcaagaa ctcgctcgac attgccggcc
     6121 tgctcaagat caagggcagc gaaatccagc aacgctacac cgagctgatg atgctggccg
     6181 ccggcccgta cagcctgccg ttcatcgagg aagccatgaa cgccggctgg cagggcgacc
     6241 aggccgccgc cgccggcctg cagggttttc cggggggcga cgtggccaac gccacgctcg
     6301 cgcccagcta cttcaactac cgcaagacga ccatctacgg cggcagcaac gaagtgcaac
     6361 gcaacatcgt ggctcaaacc gtgctgggct gatcaggaga caagaacatg gatttcgatt
     6421 tttccgacga ccagcaagcc ctgcgcgagg ccgtcgccaa gtgggtcgag aaaagctacg
     6481 acttcgagcg ccgccagcaa atcgtcaagt ccggcggttt tgaccgcgcg gtctacaacg
     6541 aactggccga actgggcctg acgggcatct acgtgagcga agagtacggc ggcatgggca
     6601 tggggcccgt ggaggcgatg gtggtgctgg aagaactggg ccgcggcatc gtgaccgagc
     6661 cgctggcgca ggccttcgtc accagcgccg tcattcaggc ctatggcagc gacggggtca
     6721 agcagcagtg gctgccgaag atcgccagcg gcgaggcgct ggtggtgctg gcgcagcaag
     6781 agcgcaaggc ccgccatcaa ctcgacgttt gcgaagccaa agcggcaaaa tcgggcgaca
     6841 gttatgcgct gacagctctt aaaaacatag tgcccgcggg cgaccaggcc gacgccttcg
     6901 tggtgcccgc catgctggac ggcaagctgg cgctgttcat cgtcgagcgc gccgccaaag
     6961 gcgtcagcac gcgcggctac ctgacgcagg cggagtcgcg cgccgccgac gtgaaactcg
     7021 acaacgcgcc ggcgcaactc atcaccaccg atggcctgac ggcgctgaac ttcgcggtcg
     7081 atgtcggcat cgcctgcgtc tgcgccgaag gcgtgggcgc gatggaccag acgctgaagc
     7141 tgaccaccga gtacatgaac cagcgcaagc agttcggcgt caccatcgcc accttccagg
     7201 cgctgcgcca ccgcgtggcc gacatgaaga tgcagctgga gctggcccgc tcgatgagct
     7261 actacgccac gctaaagctg ggggcgcccg aggccgaacg acgccaggcc ctctcgcgcg
     7321 ccaaggtgca actgggcaac tcgatgcgct ttgtcggcca gcaatcggtg cagttgcacg
     7381 gcggcatcgg cgtgaccgac gaatacgccg gcagccacta cttcaaggtg ctgacgcagc
     7441 tggagatgac ctacggcgac acgctgcacc acttgggcga ggtgagccag cgcatgacgg
     7501 agacggccgg cgttttcgcc tgaccgcagc gccgctgcga acatcggtgt tcgctggcgg
     7561 ggcgccgact caagtcaccg gcgcggggtt gaacagcacc agcgcgttgt gcagcttcca
     7621 gcgctctgcc caggtcttct ggcggccgct ggccacatcg agcagcagtt caaacatctc
     7681 caggcccgcc tgctcgatgg tctgctcgcc gtcggcgatg cggccggcgt tcacatccat
     7741 caggtcgtgc cagcgccgcg ccaggtcgct gcgcgtggcc actttcacca ccggcacggc
     7801 cgccagccca tacggtgtgc cgcggccagt ggtaaacacg tgcaggttca tgccggccgc
     7861 cagttgcagc gtgccgcaaa tgaagtcgct ggccggcgtg gcggcaaaca ccaggccggt
     7921 ctgatcggcc ttgagctttt cgcccggcgg cagcacgctg gcaatcgccg cgctgccgct
     7981 cttgatgacg ctgcccatcg ccttctcggt gatgttcgac aggccgccgg ccttgttgcc
     8041 gggcgtggtg ttggcggcgc ggtccacgcg gccgcggtcc aggtactggt cgtaccagcc
     8101 cagttcgcgc acgatggcct cggccaccgc gggcgtggcg gcgcgtgagg tcagctgatc
     8161 gaccgcatcg cgcacctcgg tgttctcgct gaacatgagc gtgccgccgg cgcgcaccac
     8221 caggtcggcc atcacgccga cagcggggtt ggccgtgacg cccgagaagg catcgctgcc
     8281 gccgcactgc acgcccacca ccagcttggc tgcaggcacc gtctggcgcc ggcgcgcgtt
     8341 caggcgctcc aggatctgcc gcgcgcgctg cacgatgtcg tcgatcatgg acatgaagcc
     8401 gacatgcgaa tcgtcctgca aacacacgtt gttgagcgcc gcgccttcgg gccgcagccc
     8461 gatggtgccc ggcgggatga ggcggtcggg ctgcagcttt tcgcagccca ggctgaccat
     8521 cagcagctcg ccgccgaagt tcgggttctg gctgatgtgg cgcaaggtgc gctgcggaat
     8581 ctcggcgccc ggcgcgtcga tggcgacgcc gcagccgtag gtgtgctcca gcgccaccac
     8641 gccatccacg ttgggaaaca gcggcagcag ttcggtgcgg atgcgctcgg ccgcgatgcc
     8701 gaccacgccg gctacgcact gcaccgtggt ggtgatggcc agcaggttgc gcgtgccgac
     8761 cgaaccatcg gcgttgacat agccctcgaa cgtgtagcct tgcagcggcg cccatgccgg
     8821 cgcgggcacc gtggccatgg gcagcccgtc aagcacgcgc gcggccggaa tgtccagcag
     8881 acgctcgggc acccagctcc cggcggcaat ggcccggttg gcgtagccca ccggcacgtt
     8941 gtagcggcgc accacgccgc cggcctcgat gtccgtcagc gccactttgt ggccctgcgg
     9001 taccttgtcg cgcagcgtaa gaccatcggg cagctgcgtg cccgagggca agccgccgtc
     9061 gttggccacg atggccacgt tgtcggccgc gtgcaggcgg atggcgagcg gtgggcgtgg
     9121 ggtagtcatt gcggatcgtg attcagttgg acatttttca gtcaggctcg gcggacgcgg
     9181 gctcgcatcc tcgccccttt cgccctgagc cggtcgaagg gcatgctcga acgagaacgg
     9241 gcttcgacaa gctcagcccg aacggataaa aacgaaccat ccggacctat ttacggcgcc
     9301 tgcaccgcac tcggcatcgg ctgcccggcc acaaacgcat ccaggttgtc cagcacgcgc
     9361 tgcgccatgg cctggcgcgt ctgcaccgtg gcgctggcga tgtggggcac cagcaccacg
     9421 ttgtccagcg cgaagaatt
//
LOCUS       DAOMVO010000066         9368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1848_length_9368_cov_15.596049, whole genome shotgun sequence.
ACCESSION   DAOMVO010000066 DAOMVO010000000
VERSION     DAOMVO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1848_length_9368_cov_15.596049"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..483)
                     /locus_tag="PKV30_04985"
     CDS             complement(91..483)
                     /locus_tag="PKV30_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29608.1"
                     /translation="MNRCSKSIVTSIFIAAGACLISAGALFHSEAQAQRMFPQKVQRG
                     KITFTGTREVMLNDRPERLAPGVVVRNERNTVALTGSLRGNSFTVNYLRDPMGLVREV
                     WILTPAEAERPVRGQAMMTERETPVYAN"
     gene            621..2000
                     /locus_tag="PKV30_04990"
     CDS             621..2000
                     /locus_tag="PKV30_04990"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRL29609.1"
                     /translation="MTISALDLFKIGIGPSSSHTVGPMRAGHAFVQAAAQAGLLGAAR
                     RVQVRLYGSLAATGIGHGTDRAVVAGLMGLQPDGVDPDQMADAVDAVRLDGQLALAGT
                     HVVPFNWPQDLQLLPVNLPWHPNALRLALHGDDGVLLENTYYSVGGGFIVDEAQVAAG
                     DGAGASTPVELPYTSGEQLLALCQQHGLRVADLVLRNERAWRPDADTRSALLGIWKAM
                     KACVARGLKYEGLLPGGLDVPRRAPAMHARLNARDARPNLITQTFAAMDWVNLYALAV
                     NEENAAGGRVVTAPTNGAAGIIPAVLHFFMDFQRGAGDDDVVDFLLAATAIGVLIKQN
                     ASISGAEVGCQGEVGSACAMAAAGLTQVLGGTPAQVENAAEIGLEHNLGLTCDPIGGL
                     VQVPCIERNAMAAVKAINAAQLALAGDGTHKVSLDAAIHTLRETGRDMHDKYKETSRG
                     GLAVAYTEC"
     gene            2104..3360
                     /gene="sstT"
                     /locus_tag="PKV30_04995"
     CDS             2104..3360
                     /gene="sstT"
                     /locus_tag="PKV30_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HRL29610.1"
                     /translation="MTSPEPSAPTLWTRIHRVPLVLQIAAGMLLGIVLALVWPAAARS
                     VALLGDVFVMALKSVAPVLVLVLVAASIASHKRGHDTRIRPVLLLYVLGTLGAALIGV
                     AASFLWPTTLALTAPAAAGAPPQAVGEVLKNLLLSAVTNPVKALLDANYIGLLAWGVA
                     LGVALRHARESTQQVLLDGADALSKVVHWVIRLAPLGIFGLVAGTLATSGFDALLGYT
                     RLLAVIVGCMVAVALLLNPLIVWLKTRRNPYPLVFTCLRESGVTAFFTRSSAANIPVN
                     LALCERLKLDEDTYSVSIPLGATINMAGAAITISVMSLAAAHTLGVRVDFGTALLLSV
                     VSTLAACGASGVAGGSLLLIPMATSLLGLPTDVGMQVVAIGFVISVVQDSCETALNSS
                     TDVVFTAAALAPPAPAPLVPTDLREV"
     gene            3566..6061
                     /locus_tag="PKV30_05000"
     CDS             3566..6061
                     /locus_tag="PKV30_05000"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRL29611.1"
                     /translation="MKRDSLPGRLHQFDVQPISRDVLAEKYFKPGESSLEQLYGRVAR
                     ALASVEKPELRAEWEQKFLTNLHVGAIGAGRIMSAAGTDIQATLINCFVQPVGDAIQG
                     VDDEGYPGIYEALREAAETMRRGGGVGYDFSRIRPKGAFVKGTHSIASGPCSYMNVFD
                     QSCSTVESAGSRRGAQMGVLRIDHPDVAEFITAKRTPGRWNNFNVSVGVSDAFMQAVA
                     NGTDWALVHKAKPGQKVLEGGGYQRADGLWVYRKLPARELWDTIMQSTYDFAEPGILF
                     LDQIGRDNNLNYCEKIEATNPCGEQPLPPYGCCDLGPIILTNFVRNPFGAGGDAAFDF
                     DAFEQVVATQVRALDNVLDVTFWPLEQQRNESADKRRIGVGFTGLGNALTMLKLRYDR
                     EDGRAMATEIARRMRDAAYRASVELAKEKGAFPKFKADGYLAEGTFASRLPDDIQADI
                     RQHGIRNSHLLSIAPTGTVSLAFADNASNGIEPAFSWGYKRNKREADGSKSSYTVEDH
                     AFRLYRSLVDSTVSSDDTGKLPDYFVNALEMSAQDHVAMMEAVQPFVDTSISKTVNIP
                     EDYPYDSFKDLYRQAWQAGLKGLATYRPNSILGAVLETTPAKKDEPAAPASNAPAPAP
                     AVPYDPMRSVIEKRPAGGLPSVTEKIEYWTSEGQQRLYLIVSFLPVPAADGKGTVERA
                     IEFFMPVGQSGESQQWITATMRMLSLAARGGFLDRALSDMQKVTWDRGPVRLGTYEKA
                     DGTHVPRWHDSEVAAIGYAVENLIANRQKAAQASLFDADELPAVEPQVAPAAMVPTSV
                     MAGKKCPECGAHAMIRKDGCDYCTQCGFVGSCG"
     gene            complement(6119..6194)
                     /locus_tag="PKV30_05005"
     tRNA            complement(6119..6194)
                     /locus_tag="PKV30_05005"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6159..6161),aa:His,seq:gtg)
     gene            complement(6347..7048)
                     /locus_tag="PKV30_05010"
     CDS             complement(6347..7048)
                     /locus_tag="PKV30_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRL29612.1"
                     /translation="MNDRTQTLAYTDVARGGVTTPEQRNKVLRNTYWLLALSLLPTVL
                     GAWIGVSTGITRSLSGGLGLVVFLGGAFGFMFAIEKTKNSAAGVPVLLAFTFFMGLML
                     SRLIAMVLGFKNGSELVMTAMGGTAGVFFVMASLASVIKRDISGMGKWLFVGALVVFV
                     GAIVNVFVGSTVGMMVISTLVIGVFSLYMLYDLKQIIDGGETNYISATLALYLDIFNV
                     FQGMLALLGLGGSSD"
     gene            7261..7665
                     /locus_tag="PKV30_05015"
     CDS             7261..7665
                     /locus_tag="PKV30_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRL29613.1"
                     /translation="MKTKSFLEATEVRAVVAAAEAEALKQQWAMTIAVVDDGGHLLHL
                     HRMDGAAPVSAHIAPGKARSAALGRRESKGYEDMINGGRTAFLSAPLDGMLEGGVPIM
                     KDGQCIAAIGVSGAKPAEDTQVAKAGIAALGL"
     gene            complement(7867..8688)
                     /locus_tag="PKV30_05020"
     CDS             complement(7867..8688)
                     /locus_tag="PKV30_05020"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRL29614.1"
                     /translation="MPERTVFFISDGTGITAETFGNAILAQFEFTPRRVRLPFIDTAD
                     KAHQAVRQINHTGEVEGKRPIVFTTLVDMEILSIIRAGCKGMLLDMFGTFVAPIEQEI
                     GIKSHHRVGRFSDVSKSQAYHDRIEAINFSLAHDDGQSNRDLEAADVILVGVSRSGKT
                     PTSLYLAMQHGLKAANYPLIPEDFERRQLPPALVPHKKKIFGLTIAPERLSEIRSERR
                     PNSRYASIENCRMEISEAEAMMRRAGIRWLSTTTKSIEEIATTILQEVRPERLSY"
     gene            8828..>9368
                     /locus_tag="PKV30_05025"
     CDS             8828..>9368
                     /locus_tag="PKV30_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRL29615.1"
                     /translation="MSALFEPTALVVPFEKLRMTDVEAVGGKNASLGEMISQLPQDVR
                     VPTGFATTAHAFRQFLAHDGLAERIRQRLATLDTEDVRALAEVGAQIRGWIEAQPFPA
                     DLEKAIRDAFATLSAGNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIDDVLHKM
                     KEVFASLYNDRAISYRVHKG"
BASE COUNT         1670 a         3058 c         3077 g         1563 t
ORIGIN      
        1 tgcgggacat gggtgtctcc gggtgaaaag cacagtggcc ggtcagcgcc ggcaaagaga
       61 aaagcacggc tgcgcgccgc gcggcacgcg tcagttggcg taaaccggcg tctcgcgctc
      121 ggtcatcatc gcttggccgc gcaccgggcg ctcggcctcg gcgggggtca agatccagac
      181 ttcgcgcacc aggcccatcg ggtcgcgcag gtagttgacg gtgaacgaat tgccgcgcag
      241 tgatccggtc agcgcgaccg tgttgcgctc gttgcgcacc accacgccgg gtgccaggcg
      301 ctcggggcgg tcgttcaaca tcacctcacg cgtgccggtg aaggtgatct tgccgcgctg
      361 caccttttgc ggaaacatgc gctgcgcctg cgcttctgaa tgaaacagcg ctccagcgct
      421 tatcaggcaa gcgccagcag ctatgaaaat cgaagtgacg atggatttcg agcagcggtt
      481 catggtatct gtccagaggc tgttgaaaga atgcggggcg aaggcgtgat tctacgcggc
      541 agcccctggc cgcgcctcgt ggccatgtgg ctcggggcgc ggcgcaccga cgtagactgc
      601 gcctattcaa aggtaccggc atgaccatca gcgccctcga cttgttcaag atcggcatcg
      661 gcccctccag ctcgcacacc gtggggccga tgcgcgcggg ccacgcgttt gtgcaggcgg
      721 cggcgcaggc cggcctgctg ggcgcagcgc ggcgcgtgca ggtgcgcctg tacggctcgc
      781 tggcggccac cggcatcggc cacggcaccg accgcgcggt cgtcgccggc ctgatgggac
      841 tgcagcctga tggggtcgat cccgatcaga tggccgatgc ggtggacgcg gtgcggctcg
      901 acggccaact ggcgctggcg ggcacacacg tggtgccctt caattggccg caagacctgc
      961 aactgctgcc cgtcaacctg ccgtggcacc ccaacgccct gcgactggcg ctgcacggcg
     1021 acgatggggt gctgctggag aacacctact actccgtcgg tggcggcttc atcgtcgatg
     1081 aggcgcaggt cgcggccggc gacggggcgg gcgcgtccac gccggtcgag ttgccctaca
     1141 cctcgggcga gcagttgctg gcgctgtgcc agcagcacgg cctgcgcgtg gccgatctgg
     1201 tgctgcgcaa cgagcgcgcc tggcgccccg atgccgacac gcgctccgcg ctgctcggca
     1261 tctggaaggc catgaaggct tgcgtggcgc gcggcctgaa gtacgagggc ctgctgcccg
     1321 gcggactgga cgtgccgcgc cgcgcgcccg ccatgcacgc gcggctgaac gcgcgcgacg
     1381 cccgccccaa cctgatcacg cagacctttg ccgccatgga ctgggtcaac ctgtacgccc
     1441 tggccgtcaa cgaagagaat gccgccggcg gccgcgtggt gacggcgccg accaatggtg
     1501 cggccggcat catcccggcg gtgctgcatt tcttcatgga ctttcagcgc ggcgcgggcg
     1561 atgacgacgt ggtcgatttc ctgctggccg cgaccgcgat tggcgtgctt atcaagcaga
     1621 acgcctcgat ctcaggcgcc gaagtcggtt gccagggcga ggtcggctcg gcctgcgcca
     1681 tggcggcggc gggcctcacg caggtgctgg gcggcacgcc ggcgcaggtc gagaatgcgg
     1741 ccgagatcgg gctcgaacac aacctgggcc tgacctgcga ccccatcggc ggcctggtgc
     1801 aggtgccgtg catcgagcgc aacgccatgg cggccgtgaa ggccatcaac gccgcgcagc
     1861 tggcgctggc gggcgacggc acgcacaagg tgagcctgga tgccgccatc cacacgctgc
     1921 gcgaaaccgg ccgcgacatg cacgacaagt acaaggaaac ctcgcgcggc ggcctggccg
     1981 tggcatacac cgaatgctga cggctggcgc tcaaggcttc aagaaaaaca ggccgccagc
     2041 gcttgtccag cgtgcgcaag cagctatcaa tatcagagta ttcgaacttt ggaggaaccc
     2101 cgcatgactt cgcctgagcc ttccgcaccg acgctgtgga cacgcatcca ccgcgtgccc
     2161 ctggtgctgc agatcgccgc cggtatgctg ctcggcatcg tgctggcgct ggtgtggcca
     2221 gcggcggcgc gctcggtggc gctgctgggc gacgtgttcg tgatggcgct gaaatcggtg
     2281 gcgccggtgc tggtgctggt gctggtggcc gcctcgattg cctcgcacaa gcgcggccac
     2341 gacacgcgca tccggccggt gttgctgctt tacgtgctgg gcacgctggg cgcggcgctg
     2401 atcggcgttg ccgccagctt cctgtggccc accacgctgg ccttgacggc gccagccgcc
     2461 gccggcgcgc caccgcaggc cgtcggagag gtgttgaaga acctgctgct gtccgccgtg
     2521 accaacccgg tcaaggcgtt gctcgacgcc aactacatcg gcctgctggc ctggggcgtg
     2581 gcgctgggcg tggcgctgcg ccatgcgcgc gaaagcacgc agcaggtgct gctggacggg
     2641 gccgacgcgc tctcgaaagt ggtgcactgg gtgatccggc tggcgccgct gggcatcttc
     2701 gggctggtgg cgggcacgct ggccaccagc ggcttcgacg cgctgctggg ctacacgcgg
     2761 ctgctggcgg tgatcgtggg ctgcatggtc gccgtggcgc tgctgctcaa cccgctcatc
     2821 gtctggctga agacgcggcg caacccgtac ccgctggtct tcacctgcct gcgcgagagc
     2881 ggcgtgacgg cgttctttac ccgttcgtcg gccgccaaca tccccgtcaa cctggcgctg
     2941 tgcgagcggc tcaagctgga cgaggacacg tattcggtgt cgatacccct cggcgccacc
     3001 atcaacatgg cgggcgcggc catcaccatt tcggtcatga gcctggcggc cgcgcacacg
     3061 ctgggggtgc gcgtggactt tggcaccgca ctgctgctgt cggtggtgtc cacgctggcg
     3121 gcctgcggcg cctcgggtgt ggccggcggc tcgttgctgc tgattcccat ggcaaccagc
     3181 ctgctcggcc tgccgaccga cgtcggcatg caggtggtgg ccatcggctt tgtcatcagc
     3241 gtggtgcagg actcgtgcga gacggcgctc aactcgtcga ccgacgtggt cttcaccgcc
     3301 gccgcgctgg ccccacccgc ccccgcgccg ctggtgccga cggacttgcg cgaggtttga
     3361 agcgccgatg gtcgaatcga cgatttgatg gaccaagaaa taggcctctg gcgcttatcc
     3421 atcaagcgct agcagctatc tatgtgatag caaacaacat gatctatcac agaaattttc
     3481 cagatggcgg agtggcgcgg tgcaagaatg gatggcttgg ctgccgggtg cctgagcccg
     3541 gcagcgtcga ccccggagtg accgcatgaa aagagattcc ctgcccggcc gcctgcacca
     3601 gttcgacgtc cagcccatca gccgcgacgt gctggccgaa aagtacttca aacccggcga
     3661 aagcagcctc gaacagctgt acggccgcgt ggcgcgcgcc ctggccagcg tcgaaaaacc
     3721 cgagctgcgc gccgagtggg aacagaagtt cctcaccaac ctgcacgtgg gcgccatcgg
     3781 tgccggccgc atcatgagcg cggcgggcac cgacatccag gccacgctga tcaactgctt
     3841 tgtgcagccc gtgggcgacg cgattcaggg cgtcgacgac gagggctacc ccggcatcta
     3901 cgaagcgctg cgcgaagccg ccgaaaccat gcgtcgcggc ggcggcgtgg gctatgactt
     3961 ctcgcgcatc cggcccaagg gcgccttcgt caagggcacg cactccatcg ccagcggccc
     4021 gtgcagctac atgaacgtgt tcgaccagtc gtgctccacg gttgagagcg ctggatcgag
     4081 gcgtggcgcg cagatgggcg tgctgcgcat cgatcacccc gatgtggccg agttcatcac
     4141 cgccaagcgc acgccgggcc ggtggaacaa tttcaacgtc tcggtcggcg tgagcgacgc
     4201 cttcatgcag gccgtggcca acggcaccga ctgggcactg gtgcacaagg ccaagccggg
     4261 ccagaaggtg ctggagggcg gcggctacca gcgcgccgac ggcctgtggg tgtaccgcaa
     4321 gctgcccgcg cgcgagttgt gggacaccat catgcagtcg acctacgact tcgccgagcc
     4381 gggcattttg ttcctcgacc agatcgggcg cgacaacaac ctgaactact gcgagaagat
     4441 cgaggcgacc aacccgtgcg gcgaacagcc acttccgccc tacggctgct gcgatctcgg
     4501 ccccatcatc ctgaccaact tcgtgcggaa cccgtttggc gcgggcggcg acgcggcgtt
     4561 tgactttgat gctttcgagc aagtcgtggc cacgcaagtg cgcgcgctcg acaatgtgct
     4621 cgacgtcacc ttctggccgc ttgagcagca gcgcaacgag agcgcagaca agcgccgcat
     4681 cggcgtcggt ttcaccggcc tcggcaatgc cttgaccatg ctcaagctgc gctacgaccg
     4741 cgaagacggc cgcgccatgg ccaccgagat tgcgcgccgc atgcgcgacg cggcgtatcg
     4801 cgcgtcggtc gagctggcca aagagaaagg cgccttcccc aagttcaagg ccgacggcta
     4861 cctggccgaa ggcacgtttg ccagccgcct gcccgacgac atccaagccg acattcgcca
     4921 gcacggcatt cgcaacagcc acctgctgtc gatcgcgccc accggcaccg tcagcctggc
     4981 ttttgccgac aacgcgagca acggcatcga gccggcgttc agctggggct acaagcgaaa
     5041 caagcgcgag gccgacggca gcaagagcag ctacaccgtg gaagaccatg ccttccggtt
     5101 gtatcgctcc ctggttgata gcacggtatc aagcgacgat acgggcaaat tgcccgacta
     5161 cttcgtcaac gcgctggaga tgagcgcgca ggaccacgtc gccatgatgg aagcggtgca
     5221 acccttcgtc gacaccagca tcagcaagac ggtcaacatc cccgaggact atccgtacga
     5281 cagcttcaag gatttgtacc gacaggcctg gcaggccggc ctgaaagggc tggccaccta
     5341 ccgccccaac agcatcctgg gcgcggtact ggagaccacg ccggccaaga aagacgagcc
     5401 ggcggcgccc gcctcgaacg cgccagcgcc cgcgcccgcc gtgccgtacg acccgatgcg
     5461 ctcggtgatc gaaaagcgcc ccgccggcgg cctgccttcg gtcaccgaga agatcgagta
     5521 ctggaccagc gaaggtcagc aacgcctgta cctcatcgtc tcattcttgc cagtgccggc
     5581 ggccgatggc aagggcacgg tcgagcgcgc catcgagttc ttcatgccgg tcggccagag
     5641 cggcgaatcg cagcagtgga tcaccgccac catgcgcatg ctgagcctgg ctgcacgtgg
     5701 cggttttctg gaccgcgcgc tgtcggacat gcaaaaggtc acgtgggacc gtggcccggt
     5761 gcgcctgggc acctacgaaa aagccgacgg cacccacgtg ccgcgctggc acgacagcga
     5821 agtcgccgcc atcggctacg cggtggagaa cctgatcgcc aaccgccaga aagccgcgca
     5881 ggcctccttg ttcgacgccg atgaactgcc cgccgtcgag ccgcaagttg ctccggccgc
     5941 gatggtgccc accagcgtca tggccggcaa gaaatgcccc gaatgcggtg cgcacgccat
     6001 gatccgcaaa gacggctgcg actactgcac gcagtgcggt tttgtcggca gctgcggcta
     6061 ggccggccgc gcaaaaaaag cccgctgcga ttgcagcggg cttttttcat ggtgcatatg
     6121 gggtggctga cgggactcga acccgcgaca acaggaatca caatcctgga ctctaccaac
     6181 tgagctacag ccaccgtgaa gaacccggga ttataggaca tgtgtcggct gaagcccgca
     6241 cccgtcgatt gcgccagcct cgccagaagg catgaatgcc cacaaaaaaa ggggccgcag
     6301 cccctttttt tgatcgaacg cccgttgccc ggcgtcggca aatacgtcaa tcgctgctgc
     6361 cgcccaagcc cagcagcgcc agcatgccct gaaacacgtt gaagatgtcc aggtacagcg
     6421 ccagcgtggc gctgatgtag ttggtttcgc cgccgtcgat gatttgcttg aggtcgtaca
     6481 gcatgtacag gctgaacacg ccgatgacca gggtgctgat caccatcatg ccgaccgtgg
     6541 agccgacgaa cacgttgacg atggcgccca caaacaccac cagcgcgccg acaaacagcc
     6601 atttgcccat gcccgagatg tcgcgcttga tgacgctggc caggctggcc atgacgaaga
     6661 acacgccggc cgtgccgccc atggcagtca tcaccagttc ggagccgttc ttgaagccca
     6721 gcaccatcgc gatcaggcgc gacagcatca ggcccatgaa gaaggtgaag gccagcagca
     6781 ccggcacacc agcggccgag ttcttggtct tttcgatcgc gaacatgaag ccgaaggcgc
     6841 cgcccaggaa cacgacgagg ccgaggccgc cgctcaggct gcgcgtgatg ccggtggaca
     6901 cgccgatcca ggcgcccagc accgtcggca gcaggctcag cgccagcagc cagtaggtgt
     6961 tgcgcagcac cttgttgcgc tgctccggcg tggtgacgcc gccgcgcgcg acgtcggtgt
     7021 aggccagggt ttgggtgcgg tcgttcattc aggtttctcc gtgaaggtct gggccgtggg
     7081 ccgccacctt ggcgaccatg cggttcattg tacgtagagt cgggcgtttg gaaaaaattc
     7141 cattcaaaat caaagggatg ggtccagcgg gcagaaaaat cgaggacggc cgcggcggcc
     7201 ctgcctgcgg cgtcgcctac agtggatacc tttttcaccc tctgatggga gcaccacatc
     7261 atgaaaacca aatcctttct cgaagccacc gaagtgcgcg ccgtcgtggc ggcggccgaa
     7321 gccgaagcgc tcaagcaaca atgggccatg accatcgccg tggtcgatga cggcggccat
     7381 ctgctgcacc tgcaccgcat ggatggcgcg gcgccggtca gcgcccacat tgccccgggc
     7441 aaggcgcgca gcgcggcgct gggtcggcgc gagagcaagg gctatgaaga catgatcaac
     7501 ggcggccgca ccgcgtttct ctccgcgccg ctcgacggca tgctggaagg cggcgtgccg
     7561 atcatgaagg acggccagtg cattgccgcc atcggcgtca gcggcgccaa gccggctgaa
     7621 gacacgcaag tggccaaggc ggggattgcg gcgctggggc tctgatttca ggtgtttttc
     7681 gggctccagc gcaagctggg taagcgcgag aagctattca tttagaagca agctcgtcgc
     7741 catggcctga tggaggagcg tgctgcttca gcaagcgatt tttgatggat tcaaaagcct
     7801 tttgatcttc tagcgcttgc ccagcaagcg ccagcagcta taaaaatcga agtattcacc
     7861 aatccatcag taactcaatc gctctggccg cacctcttgc aatatggtgg tcgcaatctc
     7921 ctcgatcgac ttggtggtgg tcgacagcca gcgaatgccg gcgcgccgca tcatggcctc
     7981 ggcctcgctg atttccatgc ggcagttttc gatagacgcg tagcgcgagt tgggccggcg
     8041 ttctgagcga atctcggaca gccgctcggg cgcgatggtc aggccgaaaa tcttcttctt
     8101 gtgcggcacc agcgccggcg gcagctgacg gcgctcgaaa tcctcgggga tcagcgggta
     8161 gttggccgcc ttcagcccgt gctgcatggc cagataaagg ctggtcggcg tcttgccgct
     8221 gcggctgacg ccaaccagga tcacgtcggc cgcctcaagg tcgcggttgc tctggccgtc
     8281 gtcgtgcgcc agactgaagt tgatggcctc gatgcggtcg tgataagcct gcgacttgct
     8341 cacgtctgaa aaacggccca cgcggtggtg gctcttgatg ccgatttcct gctcgatggg
     8401 cgccacgaag gtgccgaaca tgtcgagcag catgcctttg cagcccgccc ggatgatgct
     8461 gaggatttcc atgtccacca gcgtggtgaa gacgatcggc cgtttgccct ccacctcgcc
     8521 cgtgtggttg atctggcgca ccgcctggtg cgccttgtcc gccgtgtcga tgaagggcag
     8581 gcgcacgcgg cgcggcgtga actcgaactg cgccaggatg gcgttgccaa aggtctcggc
     8641 ggtgatgccg gtgccatcgg agatgaagaa gacggtgcgt tcaggcatgg gggtggctcg
     8701 cgctacatac aatccacccc attatgcgaa gccgccgcgc aatgaccacg cacaacaacc
     8761 atcaaggtca gcggcgcagc gcctgccggg cgcatcgcca acgtttttca aactccggag
     8821 ctttcccatg tctgcactgt tcgagccgac cgccctggtc gtgccctttg aaaaactcag
     8881 gatgaccgat gtcgaggcgg tgggcggcaa gaacgccagc ctcggcgaga tgatttccca
     8941 actgccgcag gacgtgcgcg tgccgacggg ttttgcgacc acggctcacg catttcgcca
     9001 gtttctggcg cacgatgggc tggccgagcg cattcgccag cggctggcga cgctggacac
     9061 cgaagacgtg cgtgcgttgg ccgaggtggg cgcgcagatt cgcggctgga tcgaggcgca
     9121 gcccttcccc gccgatcttg aaaaagccat tcgcgacgct ttcgccacgc tgagcgcggg
     9181 caacgcgcag gccagctttg cggtgcgctc atcggccacg gccgaggatt tgcccgatgc
     9241 ttcgtttgcc ggccagcagg agactttcct caacgtcgtc ggcatcgatg acgtgctgca
     9301 caagatgaag gaagtgttcg ccagcctgta caacgaccgc gccatcagct accgcgtgca
     9361 caagggct
//
LOCUS       DAOMVO010000067         9357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1852_length_9357_cov_12.317136, whole genome shotgun sequence.
ACCESSION   DAOMVO010000067 DAOMVO010000000
VERSION     DAOMVO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1852_length_9357_cov_12.317136"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..723)
                     /gene="def"
                     /locus_tag="PKV30_05030"
     CDS             complement(184..723)
                     /gene="def"
                     /locus_tag="PKV30_05030"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRL29616.1"
                     /translation="MAIHEILKMGDARLLRVAQPVESFDTDALHLLVRDMFETMRSVN
                     GAGLAAPQIGVDLQLVIFGTDAVNPRYPEAPLVPRTVLCNPVVTPLSDEEEEGWEGCL
                     SVPGLRGVVPRWRRIRYSGFDPYGDPIEREVEGFHARVVQHECDHLIGKLYPMRVRDF
                     TRFGFTDVLFPGLDATADD"
     gene            complement(772..1317)
                     /locus_tag="PKV30_05035"
     CDS             complement(772..1317)
                     /locus_tag="PKV30_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29617.1"
                     /translation="MVIQDSSSRARRVALGSAAAALVLLTGCVAVPMDAYDPGAPVVY
                     SDAPYYGSTYYGGPAYYGAPVYQQPYWGPAVSLGIYGGFGGDRGHWRGPPGRGHGHWD
                     RPPGTGGGQWHRPPGAGGGSWHRPAPGAGGGHGPRPPGAGGGHVPRPPAAGSRWQPGI
                     ENGSLGGSRGGGGVRGGRDMP"
     gene            complement(1524..2447)
                     /locus_tag="PKV30_05040"
     CDS             complement(1524..2447)
                     /locus_tag="PKV30_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRL29618.1"
                     /translation="MTGLRLGIDLGGTKIEIAALDAHGAFVLRERVPTPQGDYAATLR
                     AIAALVAQAEKRLGVAGLPLGVGIPGSLSPLTGRVRNANSTALNGQPLGDDLQALLQR
                     PVRLENDANCLALSEATDGAGAGADVVFAAILGTGVGAGVAVHGRVLRGCNGVAGEWG
                     HNPLPRADAGELAPRCWCGRAGCLELFLSGPGLAADHARASGAVLDTAAIVTAARTGD
                     GMAAASLARYAERLARSLAQVINLLDPDVIVLGGGMSNVAELYDAVPRLWAPHVFSDV
                     VRTALRPARHGDSSGVRGAAWLAAADLAGSS"
     gene            complement(2444..4576)
                     /gene="ligA"
                     /locus_tag="PKV30_05045"
     CDS             complement(2444..4576)
                     /gene="ligA"
                     /locus_tag="PKV30_05045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRL29619.1"
                     /translation="MNIEATAARLRAALNRAAHQYYTLDAPELPDAEYDKLFQELQAI
                     EAAHPELRTPDSPTQRVGGAVLAGFRPVRHAVPMLSIRTETDTTPAGAEKFDEQVRNY
                     LASEQRRIGLKANDPRHRAEPLGPVAQAVLAGAPVAYVAEPKFDGLAISLRYENGVLV
                     QAATRGDGETGEDVTQNIRTLRQIPLRLSGDAPPVLEVRGEVYMRRDDFEALNERMRA
                     RMAAGDKAAKTFVNPRNAAAGAVRQLDPAIAAERPLSFFAYGLGEVTPPSEGGPDFQS
                     HWQVLQALKSWGFPVSALARQAQGASDLIAFHADVAAERDQLPFDIDGVVYKVDALEL
                     QRELGFVSREPRWAVAHKYPAQEQLTTVQAIDVQVGRTGKLTPVAKLAPVFVGGVTVT
                     NATLHNELEARRKDVRVGDTVIVRRAGDVIPEVVSVLPDKRVAGAPEFTMPTHCPVCG
                     SDVVREEGEADHRCTGGLFCAAQRKQALLHFASRRAMDVEGLGEKLVDQLVDGNVVKT
                     LPDLYRMGFVALAALDRMAEKSARNVLDALEKSKHTTLARFLFALGIRHVGETTAKDL
                     ARHFGTLDAIMAASTDQLLQTPDVGPVVAESIHTFFQQPHNREVVEQLRAVGITWKEG
                     EPAPAALLPLAGKTFVLTGTLPTLSRDEAKEMLEAKGAKVSGSVSKKTHYVVAGEEAG
                     SKLDKARELGVPVIDEAQMQALLGGSGA"
     gene            complement(4791..5915)
                     /locus_tag="PKV30_05050"
     CDS             complement(4791..5915)
                     /locus_tag="PKV30_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HRL29620.1"
                     /translation="MSSLQVGLAVAGGLILGGVIAYNTWNARRNEPRQADPEPDAPRD
                     AVAPQERADARIEPSLHSTQPASLVAEPELDPDFEARLRPNFATTSTLALSTVPDRAP
                     GLDALIDVIVPIHLYGTQVSGDAVLAALPATRRVGSKPLAVEGREEVFDPWEHPVPGV
                     RYNYLQAGVQLANRTGPLNEIEYSEFVMKTQAVADALGGTPEFGEMLQEVARARELDQ
                     FAGQHDAQLGFLLRARQAAWSPGYVHQQAARLGFVAGVIPGRMVLPSPDPGMPPVLSL
                     VFDPQAALAEDPSHAALREVALSLDVPQVPRELEPFARLRQVVDALAQAMDGVITDDA
                     GQPLKAQTIDAIGADLDRLYDALEQHDLAAGSPQARRLFS"
     gene            complement(5912..>9357)
                     /gene="smc"
                     /locus_tag="PKV30_05055"
     CDS             complement(5912..>9357)
                     /gene="smc"
                     /locus_tag="PKV30_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRL29621.1"
                     /translation="LVGVVGPNGCGKSNIMDAVRWVLGESKASELRGESMQDVIFNGT
                     TSRKPASRSSVELVFDNADHRAGGQWAQFAEISVKRVLTRDGTSSYYINNQPVRRRDV
                     QDVFLGTGLGPRAYAIIGQGTISRIIESRPEELRLFLEEAAGVSKYKERRRETENRLS
                     DTRENLTRVEDILRELNANLDKLEKQAEVAQRYQTLQSSASLKQNQLWFLKRAEAEEG
                     QVKLKADAEKAVNDLEARVADLRHVEADLETIRQAHYAAGDQVNQAQGLLYEASAEVG
                     KLEAEIRFVVEGRQRAEQRLVQLKEQIAQWAARKAEAEDEIENLAARGAEAEEQSITL
                     AAQLEEQQGALPDLEDALRQAQAKSAEQRTAVGQVQQQIQVLAAEQRGIEEQSRQLNA
                     RRDKLSADKNALAAPDEQRLMNLQSQLSAAQESHEVTDARLHELQDSVPQLDDARRAA
                     QQAVNAESAKHADLSARLEALKALQEKVKTDGKLKPWLQKHGLDGLAGLWSRIHVEPG
                     WENALEAALRERMAALEVGRLDMVRAFASDAPPAKLSFYSPPSAALPESAGALPRLAD
                     LLRLNDAGQKALLSDWLAGCYTSDSIESALGQRERLKSGEAIYVKSGHCVTAHSVSFY
                     APDSEQAGLLARAQEIENLEKQQRAQSFISEEARNALIKAEAAYSDASQRLVSTRREA
                     AEAQQRAHELQVETLRLTQLAEQTRARSEQIAGDLSEVDASLGDLQERRVTAEARFEE
                     LDMQLADTQERHAQLDERVIEAERKLAEAREQQRTLERQAQEATFAQRSLEARRGELV
                     RTIDTAANQAASIDTEQQRAQEELTRLTDAAAQAGLQTALALKLERERALGAQRSTYD
                     DLTTKLRASDERRLQFERELDPLRQRITDLQLKEQAARLGTEQYTQLLADAQADLAAI
                     EQSITEGNVRLQGLQGDIDRLHRDIQALGAVNLAALDELTTARERKQFLDAQNADLTE
                     AMTTLEDAIKKIDAETRDLLGGTFTTVNEHFGRMFPELFGGGNARLVMTGEEILDSGV
                     QVMAQPPGKKNQTIHLLSGGEKALTAIALVFAIFQLNPAPFCLLDEVDAPLDDANTER
                     YAKLVTKMSGQGTQFLFISHNKIAMEMAEQLIGVTMQEQGVSRIVAVDMESAVGMVEA
                     A"
BASE COUNT         1336 a         3256 c         3135 g         1630 t
ORIGIN      
        1 cggacaaccg ttttacctcc tccaaaggta ggggcccgca agggccctct ttttttgagc
       61 aacagctccc actgagtcgc cttcggcgcc ttcccccaaa ggggaacgat gccagcggcc
      121 gggcaaagcc cgttccgcag catccccgca tggcctgctc cgcgcccctt tgggcttgcg
      181 tgatcagtcg tcggcggtgg cgtcgaggcc ggggaacagc acgtcggtga agccgaagcg
      241 cgtgaagtcg cgcacccgca tggggtagag cttgccgatc aggtggtcgc actcgtgctg
      301 caccacacgg gcgtgaaagc cctcgacctc gcgctcgatc gggtcgccat acggatcgaa
      361 gccgctgtag cgaatgcgcc gccagcgtgg caccacgccg cgcaggccgg gcaccgagag
      421 gcagccttcc cagccctcct cttcctcatc cgacagcggc gtgacgaccg ggttgcagag
      481 cacggtgcgc ggcaccagcg gtgcttcggg gtagcgcggg ttgacggcgt cggtgccgaa
      541 aatcaccagt tgcagatcga cgccgatctg cggcgccgcc aggccggcgc cgttgaccga
      601 ccgcatggtc tcgaacatgt cgcgcaccag caggtgcagc gcgtcggtgt cgaaggattc
      661 gacgggctgg gcgacgcgca gcaggcgcgc atcgcccatt ttcaggatct cgtggatggc
      721 catggcccga tcatcgggca ttcggcggcg ggcaagcgcg cgccgcgccg ctcaaggcat
      781 gtctcggccg ccgcggacgc ccccaccgcc acggctgccg cccaggctgc cgttttcgat
      841 gcccggttgc caccggctgc cagcggcggg cggacggggc acgtggccgc cgccggcgcc
      901 tggcggacgc ggcccatgac cgccgcccgc gcccggagcc ggccggtgcc atgaaccacc
      961 cccggcacca ggcgggcgat gccattggcc gccgccggtc cccggtggcc gatcccagtg
     1021 cccatgacca cgcccgggcg gcccgcgcca atgcccgcgg tcgccgccaa agccgccgta
     1081 gatgcccaat gacaccgccg ggccccagta gggttgctga tacaccggcg caccgtagta
     1141 ggccgggccg ccgtagtagg tcgagccgta gtacggcgca tccgaataca ccaccggcgc
     1201 gcccgggtcg taggcgtcca tcggcaccgc gacacagccc gtcagcagca ccagcgcggc
     1261 ggcggcagag cccagcgcca cacgccgagc ccgcgaagac gaatcttgaa tgaccatggc
     1321 cgctactcct gagaaaggtt tttcctatcc atggataacg ctgcgacgag acacgacgtg
     1381 caccggcttt actttagtac gtggttgtaa cccgcgccgg gcgcaggtcg tttcagcttg
     1441 ggccgtcttt aacgcatgat tctgtatcaa acaggccgcc agcgcttgat ggacaagcgc
     1501 aagcagctat atatttgata gtttcagctt gatcccgcca gatcggccgc tgccagccag
     1561 gccgcgccgc gcacgcccga cgaatcgccg tgccgcgccg gccgcagcgc ggtgcgcacc
     1621 acgtcgctga acacgtgcgg cgcccacagc cggggcaccg cgtcatacag ctcggccacg
     1681 ttgctcatgc cgccgcccag cacgatgacg tcggggtcga gcaggttgat gacctgcgcc
     1741 agcgagcgcg ccagccgctc ggcgtagcgc gccagactgg cggcggccat gccgtcgcca
     1801 gtgcgcgcgg cggtcacgat ggcggcggtg tccagcaccg cgccgcttgc acgtgcatga
     1861 tcggccgcca agcccggccc ggacaagaac agctccaggc agcccgcgcg accgcaccag
     1921 cagcgcggcg ccagctcgcc cgcatcggcg cgcggcaggg ggttgtggcc ccactcgccc
     1981 gccacgccat tgcacccgcg cagcacgcgc ccatgcaccg ccacgcccgc gcccacgcca
     2041 gtgcccagga tggcggcgaa caccacgtcg gcgcctgcgc cggcgccgtc ggtcgcttcg
     2101 gacagcgcca gacagttggc gtcgttctcc aaccgcaccg ggcgctgcag cagcgcctgc
     2161 aagtcgtcgc ccagcggctg gccgttgagc gccgttgaat tggcgttgcg cacgcggccc
     2221 gtcagcggcg acaggctgcc cggaatgcca acgcccagcg gcaaacccgc cacgcccagg
     2281 cgcttctcgg cctgcgccac cagcgcggcg atggcgcgca aggtggccgc gtagtcgccc
     2341 tgcggcgtgg gcacgcgctc gcgcagcacg aaggcgccgt gggcatccag cgcggcaatc
     2401 tcgatcttgg tgccgcccag gtcgatgccc aggcgcaggc cggtcacgcg ccgctcccgc
     2461 ccagcagcgc ctgcatctgc gcctcatcaa tcaccggcac gcccagttcg cgcgccttgt
     2521 cgagcttgct gccggcctct tcgcccgcca cgacgtagtg cgtcttcttg ctgacgctgc
     2581 ccgacacctt cgcgcccttc gcttccaaca tctccttcgc ctcgtcgcgg ctcaaggtgg
     2641 gcagcgtgcc ggtcagcaca aaggtcttgc cggccagcgg cagcagcgcg gcgggcgcgg
     2701 gctcgccttc tttccacgtg atgccgacgg cgcgcagctg ctccaccacc tcgcggttgt
     2761 gcggctgctg aaagaaggtg tggatgcttt cggccaccac cgggccgacg tcgggcgttt
     2821 gcaatagctg atcggtactg gcagccatga tggcgtccag cgtgccaaag tggcgcgcca
     2881 gatccttagc cgtggtctcg cccacgtggc gaatgccgag cgcgaacaaa aagcgcgcca
     2941 gcgtggtgtg cttcgatttt tctagcgcgt cgagcacgtt gcgcgccgat ttctcggcca
     3001 tgcggtccaa ggcggcgagc gcaacaaaac ccatacggta cagatcgggc agcgtcttga
     3061 cgacgttgcc atcgaccagc tgatcgacca gcttctcgcc cagcccttcc acatccatcg
     3121 cgcggcggct ggcaaagtgc agcaacgcct gcttgcgctg cgcggcgcag aacaggccgc
     3181 cggtgcagcg gtggtcggct tcgccttctt cgcgcaccac gtcgctgccg cagactggac
     3241 agtgcgtggg catggtgaat tcgggcgcgc cggccacgcg cttgtcgggc aacaccgaaa
     3301 ccacttcggg gatcacgtcg cccgcgcggc gcacgatgac ggtgtcgccc acgcgcacgt
     3361 ccttgcggcg cgcttccagc tcgttgtgca gcgtggcgtt ggtcaccgtc acgccgccga
     3421 caaacaccgg tgccagcttg gccaccggcg tcagcttgcc ggtgcggccg acctgcacgt
     3481 cgatcgcctg cacggtggtc agctgctctt gcgccgggta tttgtgcgcc acggcccagc
     3541 gtggctctcg gctgacaaag cccagctcgc gctgtaattc cagcgcatcg accttgtaga
     3601 ccacgccgtc gatgtcgaac ggcagctggt cgcgctcggc cgccacgtcg gcgtgaaacg
     3661 ctatcagatc agaagcgcct tgcgcttgtc tggccagcgc tgagaccgga aaaccccatg
     3721 atttcagcgc ctgcagtacc tgccagtggc tttggaaatc ggggccgcct tcgctgggcg
     3781 gcgtgacctc gccaaggcca taagcgaaaa agctcaaggg ccgctcggcg gcgatggcgg
     3841 gatcgagttg gcgcaccgca ccggccgcgg cgttgcgcgg gttcacaaag gtcttggccg
     3901 ccttgtcgcc agcggccatg cgcgcgcgca tgcgctcgtt cagcgcctcg aaatcgtcgc
     3961 ggcgcatgta gacctcgcca cgcacctcca gcaccggcgg cgcatcgccc gaaagccgca
     4021 gcgggatctg gcgcagcgtg cggatgttct gcgtcacgtc ttcgcccgtt tcaccatcgc
     4081 cgcgcgtggc ggcttgcacc agcacgccgt tttcatagcg caggctgatg gccagtccgt
     4141 cgaacttggg ctcggcgacg taggccacgg gcgcgccggc cagcacggcc tgcgcgacgg
     4201 ggccgagcgg ctcggcgcgg tggcgcgggt cgttggcctt caagccgatg cgccgctgct
     4261 cggacgccag gtagttgcgc acctgctcat cgaacttctc ggcgccggcc ggcgtggtgt
     4321 cggtctcggt gcggatgctg agcatgggca cggcgtggcg caccggcctg aagccggcca
     4381 gcaccgcgcc gccgacgcgc tgggtgggcg aatcgggcgt gcgcagctcg gggtgcgcgg
     4441 cctcgatggc ttgcagctcc tgaaacagct tgtcgtactc agcgtcgggc aactcgggcg
     4501 cgtccagcgt gtagtactgg tgcgcggcgc ggttcagggc ggcgcgcagg cgggcggcgg
     4561 tagcttcaat attcatagct gctcgcgctt gctgcacatg ggcaatctgt tgattcgatg
     4621 acaaaatgac ttcgctgcgc ttccatgacg aaatgaccac gcctcgtcat gccgcgcagc
     4681 gccgtgatcg acggcgaagc cgagatcatg caccgtcagg tgcgtcatca aaaatcatag
     4741 ctgctcgcgc ttgtcggcgg tgcgccggcg gccaaaaagg cttgtaacgc tcagctgaac
     4801 agccgccgcg cctgcggcga gccggcggcc aggtcgtgct gctccagcgc gtcgtacagg
     4861 cggtccagat cggcgccaat ggcgtcgatg gtctgcgcct tcagcggttg gccagcgtcg
     4921 tcggtgatga cgccatccat ggcctgcgcc agcgcatcca ccacctggcg cagccgcgcg
     4981 aagggctcca gctcgcgcgg cacctggggc acgtccaggc tcagcgccac ctcgcgcagg
     5041 gccgcgtgcg aagggtcttc ggccagcgcg gcctgcggat cgaacaccag gctgagcacc
     5101 ggcggcatgc cggggtcggg cgacggcagc accatgcggc cgggaatcac gcccgccaca
     5161 aacccgaggc gcgctgcctg ctggtgaacg taacccgggc tccacgcggc ctggcgcgca
     5221 cgcagcagaa agcccagttg cgcatcgtgc tggccggcaa actggtccag ctcgcgcgcg
     5281 cgcgccactt cctgcagcat ctcgccgaat tcgggcgtgc cgcccagcgc gtcggccacg
     5341 gcttgcgtct tcatcacgaa ttcggagtat tcgatttcgt tcagcggccc cgtgcggttg
     5401 gccagttgca cgccggcctg caaatagttg tagcgcacgc caggcaccgg gtgctcccac
     5461 gggtcgaaca cctcctcgcg cccttccacc gccagcggct tgctgccgac gcggcgcgtg
     5521 gccggcagcg ctgccagcac ggcgtcgcca gagacctgcg tgccgtacag gtggatcggc
     5581 acgatgacgt cgatcagcgc gtccagaccg ggcgcgcggt cgggtacggt cgagagggcc
     5641 agggtcgagg tggtggcgaa gttggggcgc aggcgcgcct cgaagtcggg gtcgagctcg
     5701 ggttcggcca ccagcgacgc aggctgcgtg ctgtgcagcg acggctcgat gcgcgcatcc
     5761 gccctttcct gcggcgccac tgcatcgcgc ggcgcatcgg gttccggatc ggcctggcgc
     5821 ggctcgttgc ggcgcgcgtt ccaggtgttg taggcgatca cgccgccgag gatcaggcca
     5881 ccggcgaccg ccaggccgac ttgcaaggaa ctcatgcggc ctccaccatg ccgacggcgc
     5941 tttccatgtc caccgccacg atgcgcgaca cgccctgctc ctgcatcgtc acgccgatca
     6001 actgctcggc catttccatg gcgatcttgt tgtggctgat gaacaggaac tgcgtgccct
     6061 gcccgctcat cttggtcacc agcttggcgt agcgctcggt gttggcgtcg tccagcggcg
     6121 catcgacctc gtccagcagg cagaacggcg ccgggttgag ctgaaagatg gcaaacacca
     6181 gcgcaatggc cgtcagcgcc ttctcaccgc ccgagagcag gtggatggtc tggttcttct
     6241 tgcccggtgg ctgcgccatg acctgcacgc cgctgtccag aatctcttcg cccgtcatca
     6301 ccagcctggc gttgccgccg ccgaacagct cggggaacat gcggccgaag tgctcgttga
     6361 cggtggtgaa ggtgccgccc agcaagtcgc gcgtctcggc gtcgatcttc ttgatggcgt
     6421 cttccagggt ggtcatggcc tcggtcaggt cggcgttttg cgcatcgagg aactgtttgc
     6481 gctcgcgcgc ggtggtcagc tcgtccagcg cggccaggtt gacggcgccc agcgcctgaa
     6541 tgtcgcggtg caggcggtcg atgtcgccct gcaatccttg caggcgcacg ttgccttcgg
     6601 tgatggattg ctcgatggcg gccagatcgg cctgggcatc ggctagcagc tgcgtgtact
     6661 gctcggtgcc cagacgcgcc gcctgctcct tcagttgcag gtcggtgatg cgctggcgca
     6721 ggggatcgag ttcgcgctcg aattgcaagc ggcgctcgtc agacgcacgc aacttggtcg
     6781 tcaggtcgtc gtaggtgctg cgttgcgcac ccagggctcg ctcgcgctcc agcttcaggg
     6841 cgagagccgt ttgcaggccg gcttgcgcgg cggcatccgt gagtcgcgtg agttcttcct
     6901 gcgcgcgttg ctgctcggtg tcgatgctgg cagcttggtt agcggcggtg tcgatggtgc
     6961 ggaccagctc gccgcggcgt gcttccaggc tgcgctgggc gaaggtcgcc tcttgcgcct
     7021 ggcgttccag cgtgcgctgc tgctcgcgcg cctcggcgag cttgcgttcg gcctcgatca
     7081 cgcgctcgtc gagctgcgcg tggcgctcct gcgtgtcggc gagctgcatg tccagctctt
     7141 caaagcgcgc ttcggccgtc acgcggcgct cttgcaagtc gcccagcgat gcatcgactt
     7201 ccgacaaatc gcccgcaatc tgctcgctgc gcgcgcgcgt ctgctcggcc agctgcgtca
     7261 ggcgcagcgt ttcgacctgc aactcgtgtg cgcgctgctg ggcttcggcg gcttcgcgtc
     7321 gcgtgctgac caggcgttgg ctggcatcgc tgtaggcggc ctcggccttg atgagggcgt
     7381 tgcgggcttc ttcgctgatg aaggactggg cgcgctgctg tttctccagg ttttcgatct
     7441 cctgcgcacg cgccagcaag cccgcctgct cggaatcggg ggcgtagaag ctgacgctgt
     7501 gcgcggtgac acagtggccg ctcttgacgt aaatggcttc gccgcttttc aggcgttcgc
     7561 gctggcccag tgcggattcg atgctatcgc ttgtgtagca accagcgagc cagtccgaca
     7621 gcaacgcctt ttgccccgcg tcgttcaggc gcagcagatc ggccaggcgc ggcagcgcgc
     7681 cggccgattc gggcagcgct gcgctgggcg ggctgtagaa gctcaatttg gccggcggcg
     7741 catcgctggc aaacgcccgc accatgtcca gccggccgac ttccagcgcg gccatgcgct
     7801 cgcgcagcgc ggcttccagc gcgttttccc aaccgggttc gacgtggatg cggctccaca
     7861 agccggccag cccatccagc ccgtgctttt gcagccaggg cttgagcttg ccatcggtct
     7921 tgaccttttc ctgcagcgct ttcagcgcct ccagccgcgc cgacaggtcg gcgtgcttgg
     7981 cgctctcggc attcacggcc tgctgcgcgg cgcggcgcgc gtcgtcgagc tgcggcacgc
     8041 tgtcttgaag ctcgtgcagg cgcgcgtctg tgacttcgtg gctttcctgc gcggcgctga
     8101 gttgcgattg caggttcatc aggcgctgct cgtcgggcgc ggccagcgcg ttcttgtcgg
     8161 cgctcagctt gtcgcgccgg gcgttcaact ggcgcgattg ctcctcgatg ccgcgctgct
     8221 cggctgccag cacctgaatc tgctgctgca cctggccgac ggcggtgcgc tgctcggccg
     8281 acttggcttg cgcctgccgc agcgcgtctt ccagatcggg cagcgcgccc tgctgctcct
     8341 caagctgcgc ggccagggtg atggattgct cttcggcctc ggcgccacgg gcggccaggt
     8401 tttcgatttc gtcttcggcc tcggccttgc gcgcggccca ctgggcaatc tgctctttca
     8461 gttgcaccag gcgctgctcg gcgcgctggc ggccttcaac gacgaagcgg atctcggctt
     8521 cgagcttgcc gacttcggcg ctcgcctcgt acagcaagcc ctgcgcctgg ttcacttgat
     8581 cgcctgcggc gtaatgcgcc tggcgaatgg tctccagatc ggcctcgaca tggcgcagat
     8641 cggccacgcg cgcctcaagg tcgttgacgg ctttctcggc gtcggccttg agcttgacct
     8701 ggccctcttc agcctcggcg cgcttcaaaa accagagctg gttctgcttg agcgacgccg
     8761 acgactgcag ggtttgataa cgctgggcga cttcggcctg cttctccagc ttgtcgaggt
     8821 tggcgttcaa ctcgcgcagg atgtcttcga cgcgggtcag gttctcgcgc gtgtcgctca
     8881 ggcggttttc cgtctcgcgc cggcgctcct tgtacttgct gacaccggcc gcctcttcca
     8941 ggaacaggcg cagctcttcc gggcgcgatt cgatgatgcg gctgatcgtg ccctggccga
     9001 tgatggcgta ggcgcgtggc ccgaggcccg tgcccagaaa cacgtcctgc acgtcgcgcc
     9061 ggcgcaccgg ctggttgttg atgtagtagc tgctcgtgcc gtcgcgcgtc agcacgcgct
     9121 tgacggaaat ttcggcgaac tgcgcccatt gaccgcccgc gcggtggtcg gcgttgtcaa
     9181 agacgagttc gacggacgag cgcgaagcgg gcttgcgcga ggtggtgccg ttgaagatga
     9241 cgtcctgcat cgactcgccg cgcagttccg acgccttgga ttcgcccagc acccagcgca
     9301 ctgcgtccat gatgttggac ttgccgcagc cgttgggccc caccacgccg accaatt
//
LOCUS       DAOMVO010000068         9346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1855_length_9346_cov_16.106447, whole genome shotgun sequence.
ACCESSION   DAOMVO010000068 DAOMVO010000000
VERSION     DAOMVO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1855_length_9346_cov_16.106447"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PKV30_05060"
     CDS             complement(<1..983)
                     /locus_tag="PKV30_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRL29622.1"
                     /translation="MSHAQETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGSVK
                     ARKASRHATSDWMEIEKQRGISVASSVMQMVYRDHVINLLDTPGHKDFSEDTYRVLTA
                     VDSALMVIDAANGVEAQTRRLIEVCRQRDTPIITFVNKMDREVRDPLDILDEVERELG
                     MPCVPMTWPVGQGKTFGGIINLRTKAMTVFQAGSEKRPQDFEVIPLTERDKLIARFGD
                     AFLAAEESMELAVGASPTWDPEAFLAAKQTPVFFGSGVNNFGVMEVLDALVDMAPAPR
                     PRTSSAIVNRQTQEQVIQPEDDTFAGVVFKVQANMDANHRDRIAFVRVASG"
     regulatory      1089..1174
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1249..2685
                     /gene="ahcY"
                     /locus_tag="PKV30_05065"
     CDS             1249..2685
                     /gene="ahcY"
                     /locus_tag="PKV30_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRL29623.1"
                     /translation="MNARLKNPVAQDSAIADINLAPWGRKEIAIAETEMPGLMAIRDE
                     FAKKKPLKGARITGSLHMTIQTAVLIETLQALGADVRWASCNIFSTQDHAAAAIAASG
                     TPVFAIKGESLKDYWDYTHRIFDFGKKGTKGEGPNMILDDGGDATLLMHLGQQAETDA
                     KVISNPKSEEEKILFAAIKAKLKEDATWYSRKSAEIIGVTEETTTGVHRLKEMSAAGT
                     LLFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVCGYGDVGKGSAQ
                     ALRALSAQVWVTEIDPICALQAAMEGYRVVTMEDAAPLADIFVTTTGNKDVITYKHMK
                     AMKDQAIVCNIGHFDNEIQVAELEEKCKWEEIKPQVDHVIFKDGKRIILLAKGRLVNL
                     GCATGHPSYVMSSSFANQTIAQIELFTHPDAYDVGKVYVLPKHLDEKVARLQLAKLNA
                     KLTELTPEQAKYIGVPKQGPYKPDAYRY"
     gene            2744..3541
                     /locus_tag="PKV30_05070"
     CDS             2744..3541
                     /locus_tag="PKV30_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRL29624.1"
                     /translation="MRADQFLVERGLAASRSQAQRLIAAGARWLSPTGWRELSKSDDL
                     PPGAQVELLDASEARYVSRGGLKLEGALRDTELDVTGWHCLDVGQSTGGFTDCLLQHG
                     AAQVIGVDVGQGQLHPRLAADERVRALHGVNARHLDASMLIAACADSTSVSGPFDSNF
                     DLIVADLSFISLTLVLPALAPLLRANGQVLLLVKPQFELQPADIGKGGIVRDASLYAQ
                     VEQRLRNACTEVALTVRHWLPSPIAGGDGNREFFIHAARAASSTSAP"
     gene            3538..4365
                     /gene="metF"
                     /locus_tag="PKV30_05075"
     CDS             3538..4365
                     /gene="metF"
                     /locus_tag="PKV30_05075"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRL29625.1"
                     /translation="MSFPLSLEFFPPKTPEGAEKLRAVRSKLYALAPDFCSVTFGAGG
                     STQDGTLDTVREIVGEGQHGVPHLSCIGQTEASIRQRLATYREAGVKRIVALRGDLPS
                     GYGMGGEFRYASDLIAFIRAETGDHFKLAVAAYPEYHPQARSPEADIDAFAAKVRAGA
                     DVGITQYFFNADAYFRFVDELRRRGVDTPIVPGIMPITNSTQLLRFSDSCGAEVPRWI
                     RLRLQAFGDDAAAIKDFGADVVAALCERLRQGGAPSLHFYTLNQSVGTLELCRRMGR"
     gene            4610..5137
                     /locus_tag="PKV30_05080"
     CDS             4610..5137
                     /locus_tag="PKV30_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRL29626.1"
                     /translation="MGLNASTSLSEPCSSAKAVHDMRCPNYTGFKPRVGGGAAPAAAS
                     DDAPGLAVPERPSLGLRGGRDLQRGVASWYGPGFHGRLTANGERFDMNELTAAHKTLP
                     FGTRVLVHNPRTGKEVVVRINDRGPFIKGRMIDLSKAAAKALGFKSRGHDAVVLREVL
                     PARANGELVASGRDS"
     gene            complement(5261..6364)
                     /gene="paaK"
                     /locus_tag="PKV30_05085"
     CDS             complement(5261..6364)
                     /gene="paaK"
                     /locus_tag="PKV30_05085"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRL29627.1"
                     /translation="MAAPRFHDLTVARVNPEAAGAVSITFAVPDELQESFHFQPGQFL
                     TLRTTIDGQEVRRNYSICSTSSKYSQHKQLEVGIRPMEGGLFSNWAATQLKAGDTLAV
                     MPPDGRFVIRKPRALHRVGFAAGSGITPILSIIASTMEDSDTAKFTLVYGNRRMSSVM
                     FNEALQDLKDKYKNRLTLIHILSRQAQEVPMLEGRIDGAKVQQLIDTLLPARSMDEVF
                     VCGPEAMIESVEAALQAAGVPADRIYSERFTSPRLEEMSLEARRAAIVQPDPEAPVGQ
                     VALTVVIDGKSHELAMRTDQHVLDVALNAGLDLPYSCKAGVCCTCRAKVLDGEVTMDK
                     NFTLEAEEMAQGFVLSCQARPMTDKLTVSYDER"
     gene            complement(6370..6966)
                     /gene="paaJ"
                     /locus_tag="PKV30_05090"
     CDS             complement(6370..6966)
                     /gene="paaJ"
                     /locus_tag="PKV30_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRL29628.1"
                     /translation="MSNATAAALTPALSQREREFDSLWRALDQLTDPEIPVITLREMG
                     ILRDVRRGADGRIEVVITPTYSGCPAMEQIADDVATTLKSEGVDGRVVTQLAPAWTTD
                     WMSEVAREKLRAYGIAPPRCTSGPAGESAIQFAPKSRAARAYQTSASGQFGMEASIAC
                     PLCGSTNTTEISHFGSTACKSLHRCLDCMEPFDHFKNI"
     gene            complement(7166..8050)
                     /gene="paaC"
                     /locus_tag="PKV30_05095"
     CDS             complement(7166..8050)
                     /gene="paaC"
                     /locus_tag="PKV30_05095"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRL29629.1"
                     /translation="MHSESDTAVAPARQAPAATKTQAPIAYLLHLADNAVVLGQRNAE
                     WCGHAPILEEDLAMANNSLDLIGQARLLYQYAAERMAGDVRAPRGAAWPAGSITEDTL
                     AYFRGDADFRNYVLLELPHTAGLVPAAGGERDWAVSLVRNFFYSALMVGVWERLQHSA
                     DAHLAAIAAKSLKEVRYHLRHSRDWLVRLGDGTPESHRRAQAAVDYLLPFTEEFWSTS
                     ALEAEALDQGTGVDVPALRAAWNQTVDEALLEAKLTRPADTKRYVPEGKRGVHSEHLG
                     YLLGEMQSLARQHPGAIW"
     gene            complement(8050..8331)
                     /gene="paaB"
                     /locus_tag="PKV30_05100"
     CDS             complement(8050..8331)
                     /gene="paaB"
                     /locus_tag="PKV30_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRL29630.1"
                     /translation="MKDWPLWEVFVRSKQGLEHKHCGSLHASDAQHALQMARDVYTRR
                     QEGVSIWVMPSHLIAASDPDEKDQLFEPASDKIYRHPTFYEIPEEVGHM"
     gene            complement(8478..>9346)
                     /gene="paaA"
                     /locus_tag="PKV30_05105"
     CDS             complement(8478..>9346)
                     /gene="paaA"
                     /locus_tag="PKV30_05105"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRL29631.1"
                     /translation="PDAYRKTLVRQISQHAHSEIVGMLPEGNWISRAPSLKRKCILIA
                     KVQDEGGHGLYLYSAAETLGTSRDEMLEALHSGKAKYSSIFNYPALNWADMGVIGWLV
                     DGAAIMNQIPLCRCSYGPYARAMVRVCKEESFHQRQGYEALLVMMKGSQAQRDMVQDA
                     VNRTWWKCLAMFGPSDKDSMHSSLGMKWGIKRISNDDLRQKFIDATVPQAEVLGVTLP
                     DPDLKWNAERGHYDHGEIDWNEFWQTVNGNGPMNRERLATRVKAHDDGAWVRDAAIAH
                     AEKQTQRALKAA"
BASE COUNT         1542 a         3006 c         3090 g         1708 t
ORIGIN      
        1 ttgccgctgg ccacgcgcac gaaggcgatg cgatcgcggt ggttggcgtc catgttggcc
       61 tgcaccttga agacgacgcc ggcaaaggtg tcgtcttcgg gctgaatcac ctgctcctgc
      121 gtctggcgat tgacgatggc ggagctggtg cgcggtctcg gcgcgggcgc catgtcgacc
      181 agcgcgtcaa gcacttccat cacaccgaaa ttgttgacgc cggagccgaa gaacacgggc
      241 gtctgcttcg cggccagaaa cgcctctggg tcccatgtgg gcgatgcgcc gaccgccagc
      301 tccatgcttt cctcggcggc cagaaaggcg tcgccaaagc gcgcgatgag cttgtcgcgc
      361 tcggtgagcg gaatgacctc gaagtcctgc gggcgctttt cgctgccggc ctgaaaaacc
      421 gtcatcgcct tggtgcgcag gttgatgatg ccgccgaagg tcttgccttg ccccacgggc
      481 caggtcatgg gcacgcaggg catgcccagt tcgcgctcca cttcgtccag gatgtccagc
      541 gggtcgcgca cctcgcggtc catcttgttg acgaaggtga tgatgggtgt gtcgcgttgg
      601 cggcaaactt cgatcaggcg gcgcgtctgc gcctcgacgc cgttggcggc gtcgatcacc
      661 atcagcgcgc tgtccacggc ggtgagcacg cggtaggtgt cttccgaaaa gtccttgtga
      721 cccggcgtgt cgagcaggtt gatgacgtgg tcgcggtaca ccatctgcat cacgctcgat
      781 gcgaccgaga tgccgcgttg cttctcgatt tccatccagt ccgacgtggc gtggcgcgat
      841 gccttgcgcg ccttcacgct gcccgcgatc tggatcgcgc cggagaacag cagcagcttt
      901 tcggtcagcg tggtcttgcc ggcgtcgggg tggctgatga tggcaaaggt gcggcggcgg
      961 cgggtttctt gggcgtgcga caaggcaaaa ggctccggcg gcgccagggt gcgacgcgct
     1021 cgacaaaatc aggcgcccaa cgcgggcgca aaggcgagat tatcgcccgc gccgatataa
     1081 tcaccgctct ttccgaggag cgttgcagcg cctgcagaca cagccaggcg tgaggctcgg
     1141 aaaccgattc cccttgcaac gacgctcatc cactgatcgc acgtggtgag cctgtctttc
     1201 ccctgacgtc ggtggtttgt cgaagccaaa gctttttgga gcaccaccat gaacgcacgt
     1261 ttgaagaacc cggtcgcgca ggattcggcc atcgccgaca tcaacctcgc gccctggggc
     1321 cgcaaggaaa tcgccattgc cgagacagag atgcccggcc tgatggcgat ccgcgatgaa
     1381 ttcgccaaga aaaaacccct gaagggcgcg cgcatcaccg gcagcctgca catgacgatc
     1441 cagaccgccg tgctgatcga aaccctgcaa gcgctgggcg ccgacgtgcg ctgggcctcg
     1501 tgcaacattt tcagcacgca agaccacgcc gccgccgcga ttgccgccag cggcacgccc
     1561 gtgttcgcca tcaagggcga gtcgctgaag gactattggg actacaccca ccgcatcttc
     1621 gacttcggca agaagggcac gaagggcgaa ggccccaaca tgattctgga cgacggcggc
     1681 gacgccacgc tgctgatgca cctgggacag caggccgaga cggatgcgaa ggtcattagc
     1741 aacccgaaaa gtgaagaaga aaaaatcctc tttgccgcca tcaaggccaa gctgaaagaa
     1801 gacgcgacgt ggtacagccg caagtccgcc gagatcatcg gcgtgacgga agaaaccacc
     1861 accggcgtgc accgcctgaa agagatgagc gctgccggca cgctgctgtt tcgcgccatc
     1921 aacgtcaacg actcggtcac caagtcgaag ttcgacaacc tgtatggctg ccgcgaatcg
     1981 ctggtcgatg gcatcaagcg cgccaccgac gtgatgattg ccggcaaggt ggccgtggtg
     2041 tgcggctacg gcgacgtggg caagggctcg gcccaagcgc tgcgcgcgct gagcgcccag
     2101 gtgtgggtga ctgagatcga ccccatctgc gccctgcaag ccgccatgga aggctaccgc
     2161 gtggtcacca tggaagacgc cgcgccgctg gccgacatct tcgtgaccac cacgggcaac
     2221 aaggacgtca tcacctacaa gcacatgaag gccatgaaag accaggccat cgtctgcaac
     2281 atcggccact tcgacaacga gatccaggtc gccgagttgg aagaaaagtg caagtgggaa
     2341 gagatcaagc cgcaggtgga tcacgtcatc ttcaaggacg gcaagcgcat catcctgctg
     2401 gccaaaggcc gcctggtgaa cctgggctgc gccaccggcc accccagcta cgtgatgtcg
     2461 tcgagcttcg ccaaccagac catcgcgcag atcgagctgt ttacgcaccc cgacgcttac
     2521 gacgtgggca aggtctacgt gttgcccaag cacctggacg aaaaagtcgc ccgcctgcaa
     2581 ctggccaagc tcaacgccaa gctgaccgag ctgacgcccg agcaggccaa gtacatcggc
     2641 gtgcccaagc aagggccgta caagccggat gcgtatcgct attgattgag tagctgctcg
     2701 cgcttgtctg gcaagcgctg gaagcttatt tgactcaaat tccgtgcgtg ccgaccagtt
     2761 cctcgtcgaa cgcggcctgg ccgccagccg cagccaggcg cagcgcctta tcgcggccgg
     2821 cgcgcgctgg ttgtcgccca ccggctggcg cgagctgtct aagagtgacg acttgccgcc
     2881 cggcgcgcaa gtcgaactgc tggatgcgtc ggaagcgcgc tacgtctcgc gcggcggcct
     2941 gaagctggag ggcgcgctgc gcgacacgga gttggatgtg actggctggc actgcctgga
     3001 cgtgggccaa tccaccggcg gctttaccga ttgcctgctg cagcacggcg cggcgcaagt
     3061 catcggcgtc gatgtggggc agggccaatt gcacccgcgc ctggccgctg acgagcgcgt
     3121 gcgggccctg cacggcgtca acgcgcggca cctcgatgct tccatgttga tagctgcttg
     3181 cgccgattcc acaagcgtca gcggcccatt tgattcaaat ttcgatctga tcgtggccga
     3241 tctgtcgttc atctcgctca cgctggtgct gcccgcgctg gcgccgttgc tgcgtgcgaa
     3301 tgggcaggtg ctgctgctgg tcaagccgca gttcgagctg caaccggccg acatcggcaa
     3361 gggcggcatc gtgcgcgatg catcgctcta cgcccaggtc gagcagcgct tgcgcaacgc
     3421 ctgtaccgaa gtggctttaa ccgtgcgcca ctggctgccc agccccatcg ctggtggcga
     3481 tggcaaccgc gaattcttca ttcatgccgc gcgggcggca tcttccacgt ctgcaccatg
     3541 agctttccct taagccttga attctttccg cccaagacgc ccgaaggcgc cgaaaagctg
     3601 cgcgccgtgc gcagcaagct gtacgcgctg gcgcccgact tctgctcggt caccttcggc
     3661 gccggcggct cgacgcagga cggcacgctc gacaccgtgc gcgagatcgt cggcgaaggc
     3721 cagcacggcg tgccacacct gtcgtgcatc ggccagactg aggccagcat ccgccagcgg
     3781 ctggcgacgt atcgtgaggc gggcgtcaag cgcatcgtcg cgctgcgcgg cgatttgccg
     3841 agcggctatg gcatgggcgg cgagttccgc tacgccagcg atttgatcgc cttcatccgc
     3901 gccgaaacgg gcgaccactt caagctggcc gtggccgcct accccgaata ccacccgcag
     3961 gcgcgctcgc ccgaagcgga catcgacgcc tttgccgcca aggtgcgcgc gggcgccgac
     4021 gtgggcatca cgcaatactt cttcaacgcg gatgcgtact tccgcttcgt cgatgaactg
     4081 cgccgccgcg gcgtcgacac gcccatcgtg ccgggcatca tgcccatcac caactcgacg
     4141 cagctgctgc gcttttcaga cagctgcggc gccgaggtgc cgcgctggat tcgcctgcgc
     4201 ctgcaggcct ttggcgacga cgcggcggcc atcaaggact ttggcgccga cgtggtggcc
     4261 gcgctgtgcg agcgcctgcg ccaggggggc gcgcccagcc tgcacttcta cacgctgaac
     4321 cagtcggtgg gcacgctgga gctgtgccgg cgcatggggc gttgatttct tataacgtta
     4381 cgtttaattt accgacgttt tcatagactg attcatctaa aagacacctt ggcgacgcca
     4441 aacgccgggt gcgccgctaa aatccagccc catgttacgg gacacctctc ccggcgcccg
     4501 cccgcgcggc gcgtcggttt cgggaagtgc gcatcgtcag ccagtcagcc gaatgttgtg
     4561 gatcggcgcg ttgtatctgg cagccagcgg gcctttgatg gcccagacga tgggcctgaa
     4621 cgcttccacc tctttgtccg agccatgctc gtcggccaag gccgtgcacg acatgcgctg
     4681 ccctaactac acgggcttca agccgcgcgt gggcggcggc gcggcgccgg ctgcggccag
     4741 tgacgatgcg cctggtctgg ctgtgccgga gcgcccgtcg ctcggcctgc gcggcgggcg
     4801 cgatctgcag cgcggcgtgg cgtcgtggta tggccccggc tttcatggcc ggctgacggc
     4861 caacggcgag cgcttcgaca tgaacgaact caccgccgcg cacaagacgc tgccctttgg
     4921 cacgcgcgtg ctggtgcaca acccgcgcac cggcaaggaa gtggtggtgc gcatcaacga
     4981 ccgcgggccg ttcatcaagg gccgcatgat cgacttgagc aaggcggcgg ccaaggcgct
     5041 gggcttcaag tcgcgcgggc acgatgcggt ggtgctgcgc gaggtgctgc cggcgcgcgc
     5101 aaacggcgag ctggtggcgt cggggcgcga cagctgagtt tggatgaaat cggccgctgc
     5161 cgcttgtatg gcaagcgcga gcagctacga ttaggatagc aaaaaaggcg cttgaggcgc
     5221 ctttttgttt gtctgcgcgc cctggcggcc acgccgcaga tcaccgctcg tcgtagctca
     5281 ccgtgagctt gtccgtcatc ggccgcgcct ggcaactcag cacaaagccc tgcgccatct
     5341 cttcggcttc gagcgtgaag ttcttgtcca tcgttacttc gccatcgagc accttggcgc
     5401 ggcaggtgca gcacacgccg gccttgcacg aatagggcag atcgagcccc gcgttcagcg
     5461 ccacatccag cacgtgctgg tcggtgcgca tggcgagttc gtgcgacttg ccgtcgatca
     5521 ccaccgtcag cgccacctgg cccacgggtg cttccgggtc gggctgcacg atggcggcgc
     5581 ggcgcgcctc gaggctcatt tcttccaggc gcggcgaggt gaagcgctcg ctgtagatgc
     5641 ggtcggccgg cacgccagcg gcctgcagcg cggcttcgac cgattcgatc atcgcctcgg
     5701 ggccgcacac gaacacctcg tccatgctcc tggcgggcag cagggtgtcg atcagctgct
     5761 gcaccttggc gccgtcgatg cggccttcca gcatgggcac ttcctgcgcc tggcgcgaca
     5821 ggatgtggat cagcgtcagg cggttcttgt acttgtcctt caagtcttgc agcgcctcgt
     5881 tgaacatcac gctgctcatg cggcggttgc cgtacaccag cgtgaatttc gcggtgtcgc
     5941 tgtcttccat cgtgctggcg atgatgctca ggatcggcgt gatgcccgac cccgccgcaa
     6001 agcccacgcg gtgcagcgcg cgcggcttcc tgatgacgaa gcggccatcg ggcggcatca
     6061 cggcgagcgt gtcgccggcc ttcagctgcg tcgccgccca gttggagaac aggccgccct
     6121 ccatcgggcg gatgccgact tcaagctgct tgtgctggct gtacttgctg ctcgtgctac
     6181 agatcgagta gttgcgccgc acctcctgcc cgtcgatggt ggtgcgcagc gtgaggaact
     6241 ggccgggctg aaagtggaag gattcctgca actcatccgg caccgcgaag gtgatcgaca
     6301 ccgcaccagc ggcctcgggg ttgacgcggg cgacggtgag atcgtgaaag cgtggggcag
     6361 ccatggtgct cagatgtttt taaagtgatc aaacggctcc atgcagtcga ggcagcggtg
     6421 cagcgacttg cacgcggtgg agccgaagtg cgaaatttcg gtggtgttgg tggagccgca
     6481 gagcgggcag gcgatcgacg cttccatacc aaattggccg ctggcgcttg tctggtaagc
     6541 gcgagcagct cttgatttgg gagcaaactg aatcgccgat tcgcccgccg ggccgctggt
     6601 gcagcgcggc ggggcgatgc cgtaggcgcg cagtttttcg cgcgcgacct cgctcatcca
     6661 gtcggtggtc caggcgggcg cgagctgcgt cacgacgcgg ccatcaacgc cctcggactt
     6721 cagcgtggtg gccacgtcat cggcgatctg ctccatggcc gggcagccgc tgtaggtggg
     6781 cgtgatgacc acctctatcc gcccatcggc accgcgccgc acgtcgcgca ggatgcccat
     6841 ttcgcgcagg gtgatgacgg ggatttcggg gtcggtgagc tgatcaagtg cgcgccaaag
     6901 gctatcgaac tccctctccc tttgggagag ggcaggggta agggccgcag cggttgcgtt
     6961 ggacacggcg ctgcccctca cccccatctg caccccacga agcagagctt cgcggggacc
     7021 ccgcgccctc tccccagagg ggcgagggag tcaataccga caccgcgcat gccagcgact
     7081 tccagcggac gcgcggccgg ggtctccccg gccgctgcgt gcgtccccct ggggggatgg
     7141 cggccgcagg ccgactcagg ggggatcacc agatcgctcc cggatgctgc cgcgccaggc
     7201 tttgcatctc gcccagcagg tagcccaggt gttcggaatg cacgccgcgc ttgccttcgg
     7261 gcacgtagcg cttggtgtcg gccgggcggg tcagcttggc ctccagcagc gcctcgtcaa
     7321 cggtctggtt ccaggcggcg cgcagggcgg gcacgtcaac gccggtgcct tgatcgagcg
     7381 cctcggcctc aagcgcgctg gtcgaccaga actcttcggt gaacggcagc aggtaatcga
     7441 ccgcggcctg cgcgcggcgg tgcgattctg gggtgccgtc gcccaggcgc accagccagt
     7501 cgcgcgagtg gcgcaggtgg tagcgcactt ccttcaggct cttggcggcg atggcggcca
     7561 ggtgcgcgtc ggcgctgtgc tgcaggcgtt cccacacgcc gaccatcagc gcactgtaga
     7621 aaaagttgcg cacgaggctc acggcccagt cgcgctcgcc accggcggcg ggcaccaagc
     7681 cagcggtgtg cggcagttcc agcagtacgt agttgcggaa gtcggcatcg ccacggaagt
     7741 aggccagcgt gtcttcggtg atcgagccgg ccggccaggc ggcgccgcgc ggtgcgcgca
     7801 cgtcgccggc catgcgctcg gcggcgtact ggtacagcag gcgcgcttgg ccgatcagat
     7861 cgaggctgtt gttggccatg gccaggtctt cctcgaggat cggcgcgtgg ccgcaccact
     7921 cggcattgcg ctggcccagc acgacggcgt tgtcggccag gtgcagcagg taggcgatgg
     7981 gcgcttgcgt ttttgtagct gctggcgctt gtctggcggg cgctacagcc gtatccgatt
     8041 ctgaatgcat cacatgtgcc ccacttcttc agggatttcg tagaacgtgg ggtggcggta
     8101 gatcttgtcg ctggcgggct cgaacaactg gtctttctcg tccgggtcgc tggcggcgat
     8161 caggtgcgag ggcatgaccc agatgctgac gccttcctgc cggcgcgtgt agacgtcgcg
     8221 cgccatttgc agcgcgtgct gcgcgtcgct ggcgtgcagg ctgccgcaat gcttgtgctc
     8281 aagaccttgc ttgctgcgga caaagacttc ccacaggggc cagtctttca tcgcgcgtgg
     8341 tgcggtgctc atcgtgtgtg tctccagggg tgggtcagtt ttgtaggctg ggttgagcaa
     8401 agcgaaaccc agcgaatcgg tggcctcgct gcttggtttc gctatcgctc aacccagcct
     8461 acgagttcct cagatgttca agcggctttg agcgcgcgct gcgtttgttt ttcagcatgg
     8521 gcgatggcgg cgtcgcgcac ccaggcgcca tcgtcgtgcg ccttcacgcg cgtggccagg
     8581 cgttcgcggt tcatggggcc gttgccgttc acggtctgcc agaactcgtt ccagtcgatc
     8641 tcgccgtggt cgtagtggcc gcgctcagcg ttccatttca aatccgggtc gggcagcgtc
     8701 acgcccagca cctcggcctg cggcacggtg gcatcgatga acttctggcg caagtcgtcg
     8761 ttgctgatgc gcttgatgcc ccacttcatg cccaggctgg agtgcatgct gtctttgtcc
     8821 gacgggccga acatcgccag gcacttccac caggtgcggt tcacggcgtc ctgcaccatg
     8881 tcacgctggg cctggctgcc tttcatcatc accagcaggg cctcgtagcc ctggcgctgg
     8941 tggaaggatt cttccttgca cacgcgcacc atggcgcgtg cgtaggggcc gtagctgcaa
     9001 cggcacaggg ggatctggtt catgatcgcc gcgccatcga ccagccagcc gatcacgccc
     9061 atgtcggccc agttgagcgc ggggtagttg aagatgctgc tgtacttcgc tttgccgctg
     9121 tgcagcgctt ccagcatctc gtcgcggctg gtgcctagcg tttcggcggc gctgtacagg
     9181 tacaggccgt ggccgccttc gtcctgcacc ttggcgatca ggatgcactt gcgcttgagg
     9241 ctcggcgcgc ggctgatcca gttgccttcg ggcagcatgc cgacgatttc agagtgcgcg
     9301 tgctggctga tctggcgcac cagcgttttg cggtaagcgt cgggca
//
LOCUS       DAOMVO010000069         9323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1866_length_9323_cov_17.555675, whole genome shotgun sequence.
ACCESSION   DAOMVO010000069 DAOMVO010000000
VERSION     DAOMVO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_9323_cov_17.555675"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..>1325)
                     /locus_tag="PKV30_05110"
     CDS             complement(96..>1325)
                     /locus_tag="PKV30_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29632.1"
                     /translation="FCVLLFGGPERAFAQTRCAFFLHSLDELDTEPERTLLLTAADFA
                     RVNPNTGAAPIFRSRRDADITLGIYARHPVLVRHTPQGEDKAWPVKYARMFDMTNDSG
                     RFLKPDELERQGWQRAPLNRWRKGKDEAWPLYVGRMIHQYDHRSASVEVNEANLKVAT
                     LSDRTGSAAKADPSAFPAPQYWVDAKAVPAPLRRTWALGFRDIARATDVRTMIAAIVP
                     GTVAGNTLPLLVDQAMGAREASLLLANFNALAFDYITRQKAQTTHLNWYILEQLPVIA
                     PARFDNPLPTAFTAAARAAKLMNGHHANPTVADFVIPQVLALSYTAHDLAPFARDLGY
                     VDAAGKPLPPIVWNDDERRARLAALDALFFWLYGLGADDAAYVMAQFPIVREQDERAF
                     GRFRTCEDVLDWLRHVV"
     assembly_gap    1326..1425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1426..4017)
                     /locus_tag="PKV30_05115"
     CDS             complement(<1426..4017)
                     /locus_tag="PKV30_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29633.1"
                     /translation="MQGQLFSQDFLLRGVRETPAWQALTDEAVASFRARLHAVFSGRD
                     ADSALNEAQTEAELISPVLGALGWDDSALPQVNLSAKRREDVPDWLLFANRQSKQQAL
                     ALPKDEQRLRHGIALLEAKRWLRALDRGDPAEPQDPDAPSSQMLRYLSRADVVSDRRV
                     KWGVLTNGAVWRLYWQDARSRAEQFFELNLAVALGVPGAQGDFDDPAPDHALRLFLLF
                     FGRAAFLPQHWDAAGRTLHAYAQTEARLYQEQVAQNLGQRVFDNVFAQLADALAKADL
                     HAQTHTIGYGQFTRPQFTPAYLDEVREAALVLLYRLLFLFYAEDRNLLPVRDERYAEY
                     SVRRIREDVRDRVDDGATFSSTLSHIWLKLQGVFLLIDKGDDTIGMPAYNGGLFHRSR
                     SPLLERTKVPDKVMAPIIDALSRRTEEVTRGWINYRDLSVSHLGGIYERLLEYALVHE
                     VQAKDDYKDKPEINRLVAQPASFARKVSGSYYTHDDLVRLVLRESVGLLAREKQAAFE
                     QRLAGWKKRATLSNPQWQALDGDPDAGMASIDPASQLLELKICDPAMGSGHFLVTLVD
                     FLADRVLEAMTSASESVNAQPWAAHLAEAGRPWQSPVLGRIAQIRRAIKQNAATHGWA
                     VTDAQLDDRHIVRRMILKKTIFGVDKNPMAVELAKTALWLHTFTVGAPLSFLDHHLQT
                     GDSLQGERFEVVERGLHALGTLFQQGEMQRLKLAADAIAQVADLTDADIGEVHQSQAL
                     AEEANRQLAPIHAVLDFWRALRWLIPGWPVAKNSQLTKLLKDQPERQAALARLLDPQL
                     NLVATLSKGELLAQDGPAQAANFLMKQARELAARETFFHWWTRFPTVFGPGGRGGFDI
                     VLGNPPWD"
     gene            complement(4070..4540)
                     /locus_tag="PKV30_05120"
     CDS             complement(4070..4540)
                     /locus_tag="PKV30_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRL29634.1"
                     /translation="MEAATLDRIDLRILAALQADGRISNLKLAETVALSPTAVLARVQ
                     RLTREGFIVGYHADLNAAKLGRGLVVFVEVLLDRTTPNVFDEFKAAVQVHDEVMECHM
                     VAGGFDYLLKTRMADMAAYRAFAGRVLWQLPGVRETRTYAVMEEVKSGTALPLG"
     gene            4667..8461
                     /gene="putA"
                     /locus_tag="PKV30_05125"
     CDS             4667..8461
                     /gene="putA"
                     /locus_tag="PKV30_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HRL29635.1"
                     /translation="MHRGEGRSHKVHAPPAAMTTANPFAALHAEPAPQSALRDAITAA
                     TRRPEAQALAPLLKQARLPPDQAQAAHDLAHRLADTLRQRKRAAGRAGIVQGLLQEFA
                     LSSQEGVALMCLAEALLRIPDKATRDALIRDKVGRGDWQAHLGQSPSLFVNAAAWGLL
                     LTGKLVATHSEAGLGSALKRLTAKGGEPLIRKGVDMAMRMMGEQFVTGETIAEALQHA
                     RGPEAEGFRYSYDMLGEAALTADDAARYLHSYEAAIHAIGRASNQRGVIDGPGISIKL
                     SALHPRYSRAQRARVLAELYPRVLQLAQLARRYDIGLNIDAEEADRLELSLDLLEKLC
                     HEPSLAGWHGIGFVIQAYQKRCPFVIDWLVDLARASGHRLMVRLVKGAYWDSEIKRAQ
                     VDGQSDYPVYTRKPHTDVSYLACARKLLAAPDAIYPQFATHNAHSVAAIVQMAGPESW
                     APGQYEFQCLHGMGEPLYEQIVSGQLGPQRPCRIYAPVGTHETLLAYLVRRLLENGAN
                     TSFVNRIADADVPIEELIADPVAQVEAELPVGAPHPRIPLPQALYGAERGNSSGMDLS
                     DEHALQALSKSLLPALDRQSLEPDLTKKPLLPRAGEGGGEGAVAITNPADRRDIVGHV
                     QNARAADVDRALQDATTLAPTWANTPPQERAAALQRAADALQADMPRLMGLLVREAGK
                     TYANAVAEVREAVDFLRFYAAQAAAQFDNATHVPLGPVVCISPWNFPLAIFTGQVAAA
                     LAAGNVVLAKPAEQTPLIAAEAVRHLHQAGVPRDALHLLIGDGKTGAALVQDPRTRGV
                     LFTGSTEVARLIQATLAERLNPDGQPVPLIAETGGQNALIVDSSALPEQVVQDVMASA
                     FDSAGQRCSALRVLCVQHNVAERVVTMLKGAMDEATIGNPAALSVDIGPVIDAEAHAG
                     IQRHIDAMRAQGHAVHQSTRGLAEAAAHGSFIPPTLIEIERLDQLQREVFGPVLHVLR
                     YRRADLDTLIDQINATGYGLTGGVHTRIDETIARVAARANVGNLYVNRNLVGAVVGVQ
                     PFGGEGLSGTGPKAGGPLYLLRLLARQPVGAERRAVQATGEADDLNENGLQRLPDVRE
                     QLQKTLQSQGHAALLDAVAKLFDSAPSPAPRVLPGPTGERNSYSLHPRRRVLCLAERN
                     EDRLLQGAAVLAVGAQALWPADAQYDWARLPVELLTHIAITPDWRAPGVDFDAVLLHG
                     DAADALNAQRVLAARPGPIVGLTVLRPGATDVPLARLLTERSTSVNTAAAGGNASLMT
                     LG"
     gene            complement(8563..9249)
                     /locus_tag="PKV30_05130"
     CDS             complement(8563..9249)
                     /locus_tag="PKV30_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRL29636.1"
                     /translation="MEMSALILAFSKSFLLALAGVLPILNPPATAPVFVNLTRDLDPA
                     RRRALATRIGRNVVLMLGSAMVIGTYVLDFFGVSIPIVRVAGGLIVAFTAWNMLYAQQ
                     VATADKAEMAQSLVDDDHVRIQAFYPMTFPLTCGPGSISAAITVGAALHSRQWPVTAA
                     NFAGGLTAMVVIGLLVLLTFRYAGRILRPLGEVGLIVFLRLTAFILLCVGVQIFWDGA
                     SELLRGLDMR"
BASE COUNT         1506 a         3127 c         3164 g         1426 t
ORIGIN      
        1 acaaggcaaa aaggcctcta gcgcccgcca gtccagcgca agcagctatc aaaacagggg
       61 caactggcgg caggtgcagg cctagccgca gtgcgtcaca ccacgtggcg cagccagtcc
      121 agcacgtcct cgcaggtgcg aaagcgccca aaggcgcgct cgtcctgctc gcgcacgatc
      181 gggaactgcg ccatcacata cgccgcgtcg tcggcgccca ggccatagag ccagaagaac
      241 agcgcatcca gcgccgccag ccgggcgcgg cgctcgtcgt cgttccacac gatgggcggc
      301 agcggcttgc cagcggcgtc cacataaccc aggtcgcgcg cgaacggcgc caggtcgtgc
      361 gcggtgtagc tcagcgccag cacctggggg atgacgaagt cggccaccgt ggggttggcg
      421 tggtggccgt tcatcagctt ggcggcgcgc gcggcggcag tgaacgcggt gggcagcgga
      481 ttgtcgaagc gagcgggggc gatgacgggg agttgctcga ggatgtacca gttcaggtgc
      541 gtggtctgag ctttctgccg cgtgatgtag tcaaacgcca gcgcgttgaa gttggccagc
      601 agcagcgacg cctcgcgcgc tcccatggct tgatcaacca gcagcggcag cgtgttgccc
      661 gccaccgtgc cgggcacgat ggcggcgatc atggtgcgca cgtcggtggc gcgggcgatg
      721 tcgcgaaagc ccagcgccca tgtgcgccgc agcggtgcgg gtacggcttt ggcatcgacc
      781 caatactgcg gcgccggaaa tgcggacgga tcggccttgg cggcgctgcc cgtgcggtcg
      841 gacaatgtgg cgaccttgag gttggcttcg ttcacctcca cgctggccga gcggtggtcg
      901 tactggtgaa tcatgcggcc cacgtacagc ggccacgcct cgtccttgcc cttgcgccag
      961 cggttcagcg gcgcgcgctg ccagccctgg cgctccagct cgtcgggctt caggaaccgc
     1021 cccgagtcgt tggtcatgtc gaacatgcgg gcgtacttca ccggccacgc cttgtcctcg
     1081 ccttgcggcg tgtgccgcac cagcaccggg tgccgcgcgt agatgcccag ggtgatgtcg
     1141 gcgtcgcgcc ggctgcggaa gatgggcgcg gcgccggtgt tggggttgac gcgggcaaag
     1201 tcggcggccg tcagcagcag cgtgcgttcg ggctcggtgt ccagctcgtc caggctgtgc
     1261 agaaagaagg cgcagcgcgt ctgcgcgaag gcgcgttccg ggccgccgaa cagcagcacg
     1321 cagaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtccc acggcggatt
     1441 gcccagcacg atgtcgaagc cgccccgccc gccggggccg aacacggtgg gaaagcgcgt
     1501 ccaccagtgg aagaaggttt cgcgcgccgc cagctcgcgc gcttgtttca tgaggaaatt
     1561 ggccgcttgc gcaggcccat cctgcgcaag cagctctcct tttgagagcg ttgccaccag
     1621 attgagttgc ggatcaagca aacgcgccag cgccgcctgg cgctcgggct ggtctttcag
     1681 cagcttggtc aactggctgt ttttggccac cggccaaccc ggaatcagcc aacgcagcgc
     1741 gcgccagaag tccagcaccg cgtggatggg cgccagttgc cggttggcct cttcagccag
     1801 cgcctgcgac tggtggactt cgccgatgtc ggcgtcggtc aggtcggcca cctgggcaat
     1861 ggcgtcggcg gccagcttca ggcgctgcat ttcgccttgc tgaaacagcg tgcccagcgc
     1921 gtgcaggccg cgctccacca cctcgaagcg ctcgccctgc aggctgtcgc cggtttgcag
     1981 gtgatggtcg aggaaggaca gcggcgcgcc gacggtgaag gtgtgcagcc acagcgcggt
     2041 cttggccagc tccaccgcca tggggttctt gtccaccccg aagatggttt tcttcaggat
     2101 catgcggcgc acgatgtggc ggtcgtccag ttgcgcgtcg gtcaccgccc agccgtgggt
     2161 ggcggcgttt tgcttgatgg cgcggcgaat ctgggcaatg cgccccagca ccgggctttg
     2221 ccaggggcgg ccggcttcgg ccaggtgcgc cgcccagggc tgggcgttga cgctttcgct
     2281 ggcgctggtc atggcttcca gcacgcggtc ggccagaaag tcgaccagcg tcaccaggaa
     2341 gtggccgctg cccatggcgg ggtcgcaaat cttcagttcc agcaactggc tggcggggtc
     2401 gatcgaggcc atgccggcat cggggtcgcc gtcgagcgcc tgccactggg ggttggacag
     2461 cgtggcgcgc ttcttccagc cggccaagcg ctgctcgaac gcggcctgct tctcgcgcgc
     2521 cagcaggccc accgattcgc gcagcaccag gcgcaccagg tcgtcgtgcg tgtaatagct
     2581 gcccgatacc ttgcgcgcaa agctggcggg ctgcgccacc aggcggttga tttcgggctt
     2641 gtccttgtag tcgtccttgg cctgcacttc gtgcaccagc gcgtattcga gcaggcgctc
     2701 gtaaatgccg cccaggtgcg agacggacag gtcgcggtag ttgatccagc cgcgcgtgac
     2761 ttcttcggtg cggcgcgaca gcgcgtcgat gatgggcgcc atcaccttgt cgggcacctt
     2821 ggtgcgctcc agcagcggcg agcggctgcg gtggaacagg ccgccgttgt aggcgggcat
     2881 gccgatggtg tcgtcgccct tgtcgatcag caggaaaacg ccttgcagct tcagccagat
     2941 atgactgagc gtgctcgaaa acgtcgcacc gtcgtccacg cggtcgcgca cgtcctcgcg
     3001 gatgcggcgc acgctgtatt cggcgtagcg ctcgtcgcgc acgggcagca agttgcggtc
     3061 ttcggcgtag aacagaaaca gcaggcggta cagcagcacc agcgcggctt cgcgcacttc
     3121 atccaagtag gccggcgtga actgcggccg cgtgaactgg ccgtagccga tggtgtgggt
     3181 ctgcgcatgc agatcggcct ttgccagcgc atcggccaat tgggcaaaca cgttgtcgaa
     3241 cacgcgctgg cccaggtttt gtgccacctg ctcctggtac agccgtgcct cggtctgcgc
     3301 gtaggcgtgc agggtgcggc cggccgcatc ccagtgttgc ggcagaaagg cagcgcggcc
     3361 gaagaacagc aggaacaggc gcagggcgtg gtcgggcgcg gggtcgtcaa agtctccctg
     3421 cgcgccgggc acgcccaggg cgaccgccag gttgagctca aaaaactgtt cggcgcgcga
     3481 gcgtgcgtct tgccaataca ggcgccacac ggcgccattg gtcagcacgc cccacttgac
     3541 gcggcggtcg ctcaccacgt cggcgcggct caaatagcgc agcatttgcg acgagggcgc
     3601 gtcggggtct tgcggctcgg cggggtcgcc ccggtcgagc gcccgcagcc agcgcttggc
     3661 ctccagcagg gcgatgccgt ggcgcaggcg ctgctcgtct ttcggcaggg cgagcgcctg
     3721 ctgtttggac tgccggttgg caaacagcag ccagtcggga acgtcctcgc ggcgcttggc
     3781 cgacaggttg acttgcggca gcgcgctgtc gtcccaaccc agcgcgccca gcacggggga
     3841 gatcagctcg gcctcggtct gcgcttcgtt gagggccgag tcggcgtcgc gcccgctaaa
     3901 caccgcgtgc aggcgggcgc ggaagctggc caccgcctca tcggtgaggg cctgccaggc
     3961 gggcgtttcg cgcacgccgc gcagcagaaa gtcttgtgaa aaaagctggc cttgcatggc
     4021 ggagggggct tacgcgcgta acagcacggc gcagtgtaag cgggggcgct cagcccaacg
     4081 gcagcgccgt gccgctcttg acctcttcca tcaccgcata ggtgcgcgtc tcgcgcacgc
     4141 cgggcaactg ccacagcacg cggccggcaa aggcgcggta ggcggccatg tcggccatgc
     4201 gggtcttcag caggtagtcg aagccgccgg ccaccatgtg gcattccatc acctcgtcgt
     4261 gcacttgcac ggcggccttg aattcgtcga acacgttggg cgtggtgcgg tccagcagca
     4321 cctcgacaaa caccaccagc ccgcgcccga gcttggccgc gttcaaatcg gcgtggtagc
     4381 cgacgatgaa gccctcgcgc gtcaggcgtt gcacgcgcgc cagcacggcg gtgggcgaca
     4441 aggccacggt ttcggccagc ttgaggttgc tgatgcggcc gtcggcttgc agtgcggcga
     4501 ggatgcgcag gtcgatgcgg tcgagggtgg cggcttccat ggccgaatat tgtgctgtcc
     4561 atcggccttg aattggctaa acattcggat tgattgactg aaaaatagcg tctttgcggt
     4621 gtgggagcgg cgttggccgc gatggaggtg gtgcctgaag cccacgctgc atcgcggcga
     4681 aggccgctcc cacaaagtcc acgcaccgcc tgccgccatg accacagcca atcccttcgc
     4741 cgccctgcac gccgaaccgg cacctcaaag cgccttgcgc gacgccatca ccgccgccac
     4801 gcgccgtcct gaggcgcaag cgctggcgcc gctgctgaag caggcgcggc tgccgcccga
     4861 tcaggcgcag gccgcgcacg acctggcgca tcgtctggcc gacaccctgc gccagcgcaa
     4921 acgcgccgcc gggcgcgcgg gcatcgtgca ggggctgctg caggagttcg cgctgtcgtc
     4981 tcaggaaggc gtggcgctga tgtgcctggc cgaggcgttg ctgcgcatcc ccgacaaagc
     5041 cacgcgcgat gcgctgatcc gcgacaaggt ggggcgcggc gactggcagg cgcatttggg
     5101 ccaaagcccg tcgctgttcg tcaacgccgc tgcctggggc ttgctgctga cgggcaagct
     5161 cgtcgccacg cacagcgagg cggggctggg cagcgcactc aagcgcctga ccgccaaggg
     5221 cggcgagccg ctgatccgca agggcgtcga catggcgatg cgcatgatgg gcgagcagtt
     5281 cgtcaccggc gagacgattg ccgaagcgct gcagcacgca cgcgggcccg aggccgaggg
     5341 ctttcgctat tcgtatgaca tgctgggcga ggcggcgttg accgccgacg atgcggcgcg
     5401 ctatctgcac agttacgaag ccgcgatcca tgccatcggg cgcgcttcca accagcgcgg
     5461 cgtgatcgac gggccgggca tctcgatcaa gctctcagcc ctgcatccgc gctacagccg
     5521 cgcgcagcgg gcgcgcgtgc tggccgagct gtacccgcgc gtgcttcagc tggcgcaact
     5581 cgcacgtcgg tacgacatcg gcctgaacat cgatgcggaa gaggcggatc ggctggagct
     5641 gtcgctcgat ttgctcgaaa agctgtgtca cgaaccgtcg ctggctggct ggcacggcat
     5701 cggcttcgtc attcaggcgt accaaaagcg ctgcccgttc gtgatcgact ggctggttga
     5761 tctggcgcgc gccagcggcc accgcctgat ggtgcggctg gtgaagggcg cgtactggga
     5821 cagcgagatc aagcgcgcgc aggtcgatgg gcagagcgac taccccgtct atacccgcaa
     5881 accgcacacc gacgtgagct atctggcctg cgcgcgcaag ctgctggcgg cgcccgacgc
     5941 catctacccg cagttcgcca cccacaacgc gcacagcgtg gcagccatcg tgcagatggc
     6001 gggccctgaa agctgggcgc caggccagta cgaattccag tgcctgcacg gcatgggcga
     6061 gccgctgtat gagcagatcg tgagcggtca gcttggcccc cagcgcccgt gccgcatcta
     6121 cgcgccggtc ggcacgcacg agacgctgct ggcgtacctg gtgcggcggc tgctggagaa
     6181 cggggccaat acgtcgtttg tcaaccgcat tgccgatgcg gacgtgccga tcgaggagct
     6241 gatcgcagac ccggtggcgc aggtcgaggc cgaactgcct gtcggcgcgc cgcacccgcg
     6301 cattccgctg ccgcaggcgc tgtacggcgc ggagcgcggc aactccagtg gcatggattt
     6361 gagcgatgag catgcgctgc aagcgctatc aaaatcgctg ctgcccgcgc ttgatagaca
     6421 atcgctggag cccgatttga ccaaaaaacc ccttctcccg cgcgcgggag aaggcggggg
     6481 tgagggggct gtcgccatca ccaaccccgc cgaccgccgc gacatcgtcg gccacgtgca
     6541 aaacgcccgc gccgccgacg tggatcgggc gttgcaagac gcgaccaccc tcgcccccac
     6601 ctgggccaac acgccgccgc aagagcgcgc cgccgccctg caacgcgccg ccgacgcact
     6661 gcaagccgac atgccgcgcc tgatgggcct gctggtgcgc gaagccggca agacctacgc
     6721 caacgccgtc gccgaggtgc gcgaggcggt cgactttctg cgcttctacg ccgcgcaggc
     6781 cgccgcgcaa ttcgacaacg ccacccacgt gccgctcggc cccgtggtct gcatcagccc
     6841 gtggaatttc ccgctcgcca tcttcactgg ccaggtcgcc gccgcgctgg ccgccggcaa
     6901 cgtggtgctg gccaaacccg ccgagcaaac gccgctgatc gccgccgagg ccgtgcgcca
     6961 tctgcaccaa gccggcgtgc cgcgcgacgc actccatctg ctcatcggcg acggcaaaac
     7021 gggcgccgca ctggtgcaag acccgcgcac acgcggcgtg ctgttcaccg gctcgaccga
     7081 agtcgcgcgc ctgatccagg ccacgctggc cgagcgcctc aaccccgacg gtcagcccgt
     7141 gccgctgatc gccgaaacgg gcggccagaa cgcgttgatc gtcgattcat ccgccctgcc
     7201 cgaacaggtg gtgcaggacg tgatggcctc ggccttcgat tccgccggcc agcgctgctc
     7261 cgctctgcgc gtgctgtgcg tgcagcacaa cgtggccgag cgcgtcgtca ccatgctcaa
     7321 gggcgcgatg gatgaggcca ccatcggcaa ccccgccgcg ctgtcggtcg acatcggccc
     7381 ggtgatcgac gccgaggcgc acgcgggcat ccagcgccac atcgacgcca tgcgcgccca
     7441 ggggcatgcc gtgcaccagt ccacgcgcgg gctggccgag gcggcggcgc acggcagctt
     7501 catcccgccc acgctgatcg agattgagcg gctcgatcag ttgcagcgcg aagtcttcgg
     7561 ccccgtgctg cacgtgctgc gctaccgccg cgccgatctg gacacgctga tcgaccagat
     7621 caacgccacc ggatacggcc tgacgggcgg cgtgcacacc cgcatcgacg aaaccatcgc
     7681 ccgtgtcgcc gcgcgggcca acgtgggcaa cctgtacgtc aaccgcaacc tggttggggc
     7741 cgtggtcggc gtgcagccgt ttggcggcga aggcttgtcc ggcaccgggc cgaaagccgg
     7801 cggcccgctg tacctgttgc ggctgctggc gcgccagccg gtgggtgcgg agcggcgcgc
     7861 ggtgcaggca acgggtgaag cggatgattt gaatgagaac gggctccagc gcttgccaga
     7921 cgtgcgcgag cagctacaaa aaacattgca atcgcaaggc cacgctgcgc tgctcgatgc
     7981 cgtcgccaag ctgttcgaca gcgcgccctc gcccgcgccg cgcgtgctgc ccggccctac
     8041 cggcgagcgc aacagctaca gcctgcaccc acgccgccgc gtgctgtgcc tggccgagcg
     8101 caacgaagac cggctgctgc aaggggccgc cgtgctggcc gttggcgcgc aggccctctg
     8161 gccggccgac gcccagtacg actgggctcg cctgccggtg gaactgctca cgcacatcgc
     8221 catcacgccc gactggcgcg cgccgggcgt tgatttcgat gccgtgctgc tgcatggcga
     8281 cgcggccgat gcgttgaatg cgcagcgcgt gctggccgcg cgccccggcc ccatcgtcgg
     8341 gctgacggtg ctgcgccccg gcgccaccga tgtgccgctg gcgcgcctgc tgaccgagcg
     8401 cagcaccagc gtcaacaccg cggcggccgg gggcaacgcc agcttgatga ccttgggttg
     8461 acggtttgcg cgagtgaatg cgctacatca acgatagctg ctggcgcttg tctggcaagc
     8521 gccagcgacc aaaatagctt gaaattcggg ggcgactggc gctcagcgca tgtccaaccc
     8581 gcgcagcagc tcactggccc catcccagaa aatctgcacg cccacgcaca gcaggataaa
     8641 ggccgtcagc cgcagaaaca cgatcaaccc cacctcgccc agcggccgca agattcgccc
     8701 ggcgtagcgg aaggtcagca gcaccagcag cccgatcacc accatcgccg tcagcccgcc
     8761 ggcaaagttg gcggccgtca ccggccactg ccggctgtgc agcgccgcgc ccaccgtgat
     8821 ggcggccgag atcgaacccg ggccgcaggt caacggaaaa gtcatggggt aaaaggcctg
     8881 gatgcgcacg tggtcgtcat cgaccagcga ctgcgccatc tcggccttgt cggcggtggc
     8941 gacctgctgc gcgtacagca tgttccacgc cgtgaaggcc acgatcagcc cgcccgccac
     9001 ccgcacgatg ggaatcgaca cgccaaagaa atccagcacg taagtgccga tgaccatcgc
     9061 gctgcccagc atcagcacca cgttgcggcc gatgcgcgtg gccagcgcgc ggcgccgcgc
     9121 ggggtcgaga tcgcgcgtca ggttgacgaa caccggcgcc gtggccggcg ggttgaggat
     9181 gggcagcacg cccgccagcg caagcagaaa gctcttgctg aaggccagga tcaaagcgga
     9241 catttccatg agcaggacag tgcgtggact tgcgtgtaaa gtgaattttt tcaggtcggc
     9301 gccgtgctgg ccgcgacctt tgt
//
LOCUS       DAOMVO010000070         9250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1889_length_9250_cov_10.937575, whole genome shotgun sequence.
ACCESSION   DAOMVO010000070 DAOMVO010000000
VERSION     DAOMVO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_9250_cov_10.937575"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKV30_05135"
     CDS             complement(<1..275)
                     /locus_tag="PKV30_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL29637.1"
                     /translation="MASPKNTFNWEDPFLLTQQLSEDERHVQEAARAYCQEKLLPRVQ
                     MAFRNETTDVAIFREMGELGLLGPTIPEQYGGAGLNYVCYGLVAREV"
     gene            379..1275
                     /locus_tag="PKV30_05140"
     CDS             379..1275
                     /locus_tag="PKV30_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL29638.1"
                     /translation="MELRHLRYFLAVAAERHFGRAAQRLGLSQPPLTVAIQQLEAEVG
                     AALFLRNSRGVQLTAAGQALVPAAQAAIDQAAQALTAARDAASGQVGRLSVGFAGTML
                     YRGLPQMLRRFQAEHPRLQLRLRELSSSEQLVELQHARLDAGFAHVAHAPEGLAQLLV
                     SSQPFVACVPAAHALARRRALPLTALAAEPLALVSRSVSPDYHARIVGACKAAGFTPQ
                     PLHELQHWLSVVSLVAQGLAVALVPAALQQAALSGAAFVPVKDPHGALPRYETRCLWN
                     PERDLPALGLFLAQVREVAQAG"
     gene            1300..1839
                     /gene="msrA"
                     /locus_tag="PKV30_05145"
     CDS             1300..1839
                     /gene="msrA"
                     /locus_tag="PKV30_05145"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRL29639.1"
                     /translation="MSNTETIVLGGGCFWCTESVFKEVRGVLDVESGYSNGQETAPTY
                     EQVCTGRTGCNEVVKLVYDPAVIGTRQILEIFFATHDPTQLNRQGNDVGTQYRSGIYF
                     TTPEQQQVAQDLVREINQGDTAGAPVVTEVEPLANYFAAEGYHQDFFENNPTQGYCLA
                     VAAPKVAKFRKTFGKWAKA"
     gene            1970..2410
                     /locus_tag="PKV30_05150"
     CDS             1970..2410
                     /locus_tag="PKV30_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRL29640.1"
                     /translation="MYALMLFLHVLGATVWTGGHLVLALTWLPRVLRERSPEQLLRFE
                     QGYERIGMPALVLQIVTGLWMAYQMVPSVAQWLSPDTPVARAIALKLVLLLCTALIAA
                     HARLRVIPRLSADTLPLMAWHVGAVTLLSVGFVAVGVSLRFGGV"
     gene            2407..4272
                     /locus_tag="PKV30_05155"
     CDS             2407..4272
                     /locus_tag="PKV30_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29641.1"
                     /translation="MTGQPARADSAGRGFDALCLLLALVLLAALVALTLWLWQGRQRP
                     LLLAPAIGELSDCLEMAAPHAPLEAACTGERGSAAARIESTLGALGPRRSVDGRFELG
                     YTLVVPLLNLFEPKGDGWVVDRQALRRIANTVQSVDRPVVLYLFSTHFSESAPIEPVL
                     AQDPANMAHTPQGPLPPEKYLGWPLYPWSIARTDNGVTQRRDEAIRALTRTLCALPAD
                     ARGRIAGINLLGEVHHLYPDFEAGMGYNRPYVLTDYSPASRAGFRQWLRQRFKGDVAA
                     LNAYLGASFASFDQIEPPSRDIRRERLDHFWQHLDDGAAGTLAISGWAHDGALPAGRT
                     PWVRVYLDGQPVGRVPAHFVRQDVLQAKPEFGTAQVGWRYDLRFADQPPGRHRIDIAL
                     EGHDGALRLLGTRHFSVMDRDQTPPVDAPLRQPLPPMVAPGAGVQFWVDAPQDERAVF
                     YNPLVPLWHAFRGQQVVDYLAHFDHLLDQSCLADVPHRTQQIYPAEKAGWDGTRFASE
                     QSLLPFGDVRLGINLYGEAAYDDSFFDWLARSRQPVYSVTEFHPLRAMSADELRRVLL
                     RHQAHGAQSLSFFLHPPPAGGVRTEPIANPYALDPGNPLNGSDALYGAMRQVMRR"
     gene            complement(4624..5268)
                     /gene="upp"
                     /locus_tag="PKV30_05160"
     CDS             complement(4624..5268)
                     /gene="upp"
                     /locus_tag="PKV30_05160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRL29642.1"
                     /translation="MSQVSRPALHHVTHPLVQHKLTLMRNKQASSTTFRTLLGELAML
                     MAYEITRDMPTQLVEIETPLEKMQSPMIDGKKLVFASILRAGNGMLDGLLRVVPNARV
                     GHVGLYRDPATLQAVQYYYKMPADMAERDVIAVDPMLATGHSAVAAIEKLKSAGPRSI
                     KFLCLLAAPEGIATLHAAHPDVPIYTAAIDRQLNDHGYILPGLGDAGDRIFGTK"
     gene            complement(5299..5739)
                     /locus_tag="PKV30_05165"
     CDS             complement(5299..5739)
                     /locus_tag="PKV30_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRL29643.1"
                     /translation="MNTFVRLHNRSTRALERVLPDDALALAARIGIGAIFWLSGRTKV
                     DGTLRVTEGAIALFRDEFKLPLIAPELAAHLAAYAEHVFPLLLVAGLFTRGAALALLG
                     MTAVIQIFVYPDAWPTHLSWAAILLYLVRHGGGRLSLDHALGLR"
     gene            complement(5736..6500)
                     /locus_tag="PKV30_05170"
     CDS             complement(5736..6500)
                     /locus_tag="PKV30_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HRL29644.1"
                     /translation="MSTALHAFQHAFAHALRNRSGHDGPLARQPGFAVHRNTVMKGCI
                     DALHANYPAVARLVGDDWFRAAAAECARAHPPNDVRLMLYGEEFGPFLAHFAPAAELP
                     YLPDVARLDRSWSEAHVAADAPVLQSADLVALPEPALSALTLTPHPAARWHWFDDAPI
                     HTLWSYNRRCEPVPPDLAWTGEGALLTRPLGAVQDGLASRAACAFLDACAARQRLPQA
                     VRAALSAEPATDVSALLARLTTAGALSAIDPPPATP"
     gene            complement(6497..7336)
                     /locus_tag="PKV30_05175"
     CDS             complement(6497..7336)
                     /locus_tag="PKV30_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HRL29645.1"
                     /translation="MTRSLTAGIGLKPEHFEQALACRAPGVWFEVHPENYLVAGGPRL
                     AWLEAVRAEHPLSVHSVSLSLGGCEPPDGERVQALARFVRRFQPTLVSEHLAWSRWAG
                     AYLPDLLPLPRTGEALRTVCNHIDSIQRALGMSIAIENPSHYLHLEGHEWTEPEFLRE
                     LVRRTDCRLLLDVNNVAVSAHNLGLDAVAYLDAFPLDAVAEIHLAGHSADPLLGNGLW
                     IDSHDAPVAERVWSLYERVLARGGARPTLIERDGNLPAFEQLLTERQRAHDAMRAAER
                     EPA"
     gene            complement(7356..7661)
                     /locus_tag="PKV30_05180"
     CDS             complement(7356..7661)
                     /locus_tag="PKV30_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HRL29646.1"
                     /translation="MKNLPATLTALSLSALVAGAVQAQTAPMGKDMPQGGDKPKMEKC
                     YGVAMAGKNDCAAGPGTTCAGTSKADYQGNAWKHVPTGTCTTLKTPKGMGSLAPIKS"
     gene            7819..8406
                     /locus_tag="PKV30_05185"
     CDS             7819..8406
                     /locus_tag="PKV30_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL29647.1"
                     /translation="MVVTQMSLSDVESQLRSLLLQGLAGDEAAYRQFLQRLAAHLRAF
                     LGRRLFGWPDDVEDLVQECLLVMHNKRHTYQSDQPLTAWVHAIARHKLIDVLRARSGR
                     EALHDPLDDEMPLFAASDHDAQDARRDLMGLLQTLPDKQRRPIECVKIDGLSVAETAR
                     LTGLSESAVKVGIHRGLKSLAARLGHSYSATRSKA"
     gene            8403..9044
                     /locus_tag="PKV30_05190"
     CDS             8403..9044
                     /locus_tag="PKV30_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HRL29648.1"
                     /translation="MKTDDLIGMLATQAGPVRAARAGPRLAQATAGGLGGALAVLLLG
                     YGLRPGLAQDAQLPMFWVKLLIPVCVAGAGGVLAARLGRPGVAARGAWWGVVVPVVAI
                     WLLAAVQWVGAAPADRPALLWGQTWRTCIGSIGLIALPVFVAALAALRDLAPTHAAWA
                     GAAAGALAGGCGAAVYALHCPELTAPFLAVWYVAGMALPVALGAVIGPRVLRW"
BASE COUNT         1406 a         3037 c         3272 g         1535 t
ORIGIN      
        1 tccacctcgc gcgccaccag gccgtagcag acgtagttga ggccggcgcc gccgtattgc
       61 tcggggatcg tcgggccgag caggcccaac tcgcccatct cgcggaagat ggccacgtcg
      121 gtcgtctcgt tgcgaaaggc catctgcacg cgcggcagca gcttttcctg gcagtaggcg
      181 cgggcggctt cctgcacgtg gcgctcgtct tcgctcagtt gttgcgtcag cagaaagggg
      241 tcttcccagt tgaaggtgtt cttggggctg gccatggcgt gtctccgcat gtgtgtgggt
      301 ggaatcgcgc cattgtgccg ttgacatgaa tcgctgtcta atatcgaaca aggattcatt
      361 catacgcact gcctattcat ggaactgcgc catctgcgct actttctggc cgtggccgcc
      421 gagcggcact tcggccgcgc ggcccagcgc ctgggcctga gccagccgcc gctgacggtg
      481 gcgatccagc agctggaggc cgaggtgggc gctgcgctgt tcctgcgcaa cagccggggc
      541 gtgcagctga cggcggcggg gcaggcgctg gtgccagccg cgcaggccgc catcgaccag
      601 gccgcgcagg cgctgacggc cgcgcgcgac gcggccagcg ggcaggtcgg acgcctgtcg
      661 gtcggctttg ccggcaccat gctgtaccgc ggcctgccgc agatgctgcg gcgctttcag
      721 gccgaacacc cgcgcctgca actgcgcctg cgcgaactca gctccagcga gcagttggtc
      781 gaactgcagc acgcgcggct ggacgccggc ttcgcccacg tcgcgcatgc gcccgaaggg
      841 ttggcgcaac tgctggtgtc cagccagccc ttcgtcgcct gcgtgccagc ggcgcatgcg
      901 ctggcgcggc ggcgcgcgct gccgctcacc gcgctggccg ccgagccgct ggcgctggtg
      961 tcgcgcagtg tgtcgcccga ctaccacgcg cgcatcgtcg gcgcctgcaa ggcggcgggc
     1021 ttcacgcctc agccgctgca cgagttgcag cattggctga gcgtggtgtc gctggtggcg
     1081 caagggctgg cggtggcgct ggtgccggcg gcgctgcagc aggcggcgct gtccggcgcc
     1141 gcgttcgtgc cggtgaaaga tccgcacggc gcgctgccgc gctacgagac gcgctgttta
     1201 tggaaccctg agcgcgacct gccggcgctg gggttgtttc tggcgcaggt gcgcgaggtg
     1261 gcgcaagctg ggtagcatgc gggcgaagga gtcattcaca tgagcaacac tgaaaccatc
     1321 gtgctgggcg gcggctgctt ctggtgcacc gagagcgtgt tcaaggaagt gcgcggcgtg
     1381 ctggatgtgg aaagcggcta cagcaacggc caggaaacgg caccgaccta cgagcaggtc
     1441 tgcaccggcc gcaccggctg caacgaggtg gtcaagctgg tgtacgaccc ggcggtgatc
     1501 ggcacgcggc agatccttga aatctttttt gccacgcacg acccgacgca actcaaccgc
     1561 cagggcaacg acgtcggcac gcagtaccgc agcggcatct acttcaccac gcccgagcag
     1621 cagcaggtgg cccaggacct cgtgcgcgaa atcaaccagg gcgacaccgc tggcgcgccg
     1681 gtggtgacgg aggttgagcc gttggccaac tactttgccg ccgagggcta ccaccaggac
     1741 ttcttcgaga acaaccccac gcagggctac tgcctggccg tggcggcgcc caaggtggcc
     1801 aagttccgca agacgtttgg caaatgggcg aaggcctgat cggtcttcgt gctcgactcg
     1861 gttattcgca ggtaaaaagg gctccggcgc ttgtccgaca agcgcgaaca gctatgaata
     1921 gcatagtgcg tcgcggcagc ggcgggagtg ggcgacaatc gcgtctgcca tgtacgcctt
     1981 gatgcttttc ctgcacgtcc tgggtgccac cgtctggact ggtggccatc tggtgctggc
     2041 gctcacgtgg ctgccgcgcg tgctgcgcga gcgctcgccc gagcaactgc tgcgcttcga
     2101 gcaaggctac gagcgcatcg gcatgccggc gctggtgctg cagatcgtca ccggcttgtg
     2161 gatggcttac cagatggtgc ccagcgtggc gcagtggctg tcgcccgaca cgcccgtggc
     2221 ccgcgccatc gcgctcaagc tggtgctgct gctgtgcacc gcgctgatcg cggcgcacgc
     2281 ccggctgcgc gtgattccgc ggctgtcggc cgacaccttg ccgctgatgg cgtggcacgt
     2341 gggggcggtc acgttgctgt cggtgggctt cgtggcggtc ggggtgtcgc tgcggtttgg
     2401 cggcgtatga ctggccagcc agcgcgtgcc gactcggccg gccgcgggtt cgacgcgctg
     2461 tgcctgctgc tcgcgctggt gctcttggct gcgttggtcg cgctcacgct gtggttgtgg
     2521 caaggccggc agcgcccgct gctgctggcc ccggcgattg gcgagctgag cgattgcctg
     2581 gagatggccg cgccccatgc gccgctggag gccgcttgca ccggtgagcg cggctcggcc
     2641 gctgcgcgca tcgaatcgac gctgggcgcc ctgggcccgc gccgcagcgt cgacgggcgc
     2701 ttcgagctgg gctacacgct ggtggtgccg ctgctcaacc tgttcgagcc gaagggcgac
     2761 ggctgggtgg tcgaccggca ggcgctgcgc cgcatcgcca acaccgtgca gagcgttgac
     2821 cggccggtgg tgctgtactt gttttccacc cacttttctg aaagtgcgcc catcgagccg
     2881 gtgctggcgc aggatcccgc caacatggcc cacacgccgc agggcccgct gccgcccgaa
     2941 aagtatctgg gctggccgct ttacccctgg agcatcgcgc gcaccgacaa cggcgtcacc
     3001 cagcggcgcg acgaagccat ccgcgcgctc acgcgaacgt tgtgtgcgct gcccgccgac
     3061 gcgcgcggcc gcatcgccgg catcaacctg ctgggtgagg tgcaccacct gtacccggac
     3121 tttgaagccg gaatgggcta caaccggccg tatgtgctga ccgactactc gccggcctcg
     3181 cgcgcggggt ttcgccagtg gctgcgccag cgtttcaagg gcgacgtggc ggcgctcaac
     3241 gcttacctgg gcgccagctt tgcgtcgttc gaccagatcg aaccgccatc gcgcgacatc
     3301 cgccgcgagc ggctcgacca cttctggcaa cacctggacg atggcgctgc cggcacgctc
     3361 gccatcagcg gctgggcgca cgacggggcg ctgcccgccg gccgcacgcc gtgggtgcgc
     3421 gtgtacctcg acggccagcc cgttggccgt gtgccggcgc acttcgtgcg gcaggacgtg
     3481 ctgcaggcca agcccgaatt cggcaccgcc caggttggct ggcgttatga cctgcgcttt
     3541 gccgaccagc cgccgggccg ccaccgcatc gacatcgcgc tcgaaggcca cgatggcgcc
     3601 ttgcgtttgc tgggcacgcg gcatttctcc gtcatggacc gcgaccagac gccgcccgtc
     3661 gacgcgccct tgcgccagcc gctgcccccg atggtggcgc cgggtgccgg cgtgcagttc
     3721 tgggtcgacg cgccgcagga cgaacgcgcg gtgttctaca acccgctggt gcccttgtgg
     3781 cacgccttcc gcggccagca ggtggtggac tacctggcgc actttgacca cctgctcgac
     3841 caaagctgcc tggccgacgt gccgcaccgc acgcagcaga tctacccggc cgagaaggcc
     3901 ggctgggacg gcacgcgctt tgcgtcagag cagtcgctgc tgccgtttgg cgacgtgcgg
     3961 ctgggcatca acctgtatgg cgaggcggcg tacgacgatt cattcttcga ttggctggcc
     4021 cgctcgcgcc aacccgtcta cagcgtgacc gaattccacc cgctgcgcgc catgagcgcc
     4081 gacgaactgc gccgcgtgct gctgcgccac caggcgcacg gggcgcaaag cctgtcgttc
     4141 ttcctccacc caccgcctgc cggcggtgtg cgcaccgaac ccatcgccaa cccctacgcg
     4201 ctggacccgg gcaacccgct gaatggctcg gatgcgctgt atggggcgat gcggcaagtc
     4261 atgcggcggt gagtggttgc tgaacaccga ataccggacg cgaaggacgc gaaggattcg
     4321 cgaaggacgc gaaagaacag ccgaaaagtt tttgtcgagt tctttacgtc cggctgtttt
     4381 ctcttgttct agacatgacc gccagcgctt gcgggaaaag cgcgagcagc tataaaaaca
     4441 agagtcatcg ccgccccaca ccgctcgatg cgcgagagcg caagtcacca gggctgcgca
     4501 gcaagccctt cagatggccg cggagcagac cacgccggaa cgccgcggaa cgggcttggc
     4561 ccggccgcca gcgttgtccc cctgggggga aggcgccgca ggcgactcag gggggattga
     4621 gcttcatttc gtgccgaaaa tccgatcccc cgcatccccc agccccggca ggatgtagcc
     4681 gtggtcgttc agctgacgat caatggcggc ggtgtagatc ggcacgtcgg ggtgcgcggc
     4741 gtgcaatgtg gcgatgcctt ccggcgcggc gagcaggcac aggaacttga tcgagcgcgg
     4801 gccggcgctt ttgagttttt caatcgccgc caccgccgaa tgcccggtgg ccagcatcgg
     4861 gtcgacggcg atcacgtcgc gctcggccat gtcggcgggc atcttgtagt agtactggac
     4921 ggcctgcagc gtggccgggt cgcggtacag gccgacgtgg ccgacacgcg cgttgggcac
     4981 cacgcgcagc aggccgtcga gcatgccatt gccggcgcgc aggatgctgg caaacaccag
     5041 cttcttgccg tcgatcatgg ggctttgcat tttttcaagc ggcgtctcga tctcgaccag
     5101 ttgcgtcggc atgtcgcgcg tgatctcgta cgccatcagc atcgccagct cgcccagcag
     5161 ggtgcgaaag gtcgtgctgc tggcctgctt gttgcgcatc agggtgagct tgtgctgcac
     5221 cagcgggtgt gtgacgtggt gcaaggcggg gcgcgagact tgggacatgc ggccatttta
     5281 ggcagggggc cgaaatggtt aacgaagacc cagcgcatga tcgagcgaca gccggccgcc
     5341 gccgtgccgc accagataca gcaggatcgc cgcccacgac aggtgggttg gccacgcgtc
     5401 gggatagaca aagatctgga tcaccgctgt catgccgagc aaggccagcg ccgcgccgcg
     5461 cgtgaacagg ccggccacca gcaacagcgg aaacacatgc tcggcgtagg cggcgaggtg
     5521 cgccgccagc tcgggcgcga tcagcggcag cttgaattcg tcgcgaaaca gcgcgatggc
     5581 cccctcggtc acgcgcagcg tgccatcgac cttggtgcgg cccgacagcc agaagatggc
     5641 gccgatgccg atgcgcgcgg ccagcgccag cgcgtcgtcg ggcagcacgc gttccagggc
     5701 gcgggtggac ctgttgtgca ggcggacaaa ggtgttcatg gcgtggctgg gggtgggtcg
     5761 atggcgctca gcgcgccggc tgtcgtcaac cgcgcaagca gggcgctgac atcggtagca
     5821 ggctcggcgc tcaacgcggc gcggacggcc tgcggcaggc gttggcgcgc cgcgcacgca
     5881 tccagaaacg cgcacgcggc gcggctcgcg aggccgtcct gcacggcgcc gagcggccgc
     5941 gtcagcaaag caccttcgcc tgtccaggcc agatcaggcg gcaccggctc gcaccggcgg
     6001 ttgtacgacc agagggtgtg gatgggtgcg tcatcgaacc aatgccaacg cgccgcggga
     6061 tggggcgtca gggtcagcgc gctcagtgcc ggctccggca gagcgaccag atcggcggac
     6121 tgcaacaccg gcgcatcagc cgccacatgc gcttcgctcc agctccggtc cagccgagcc
     6181 acatcgggta ggtatggcaa ttcggcggcc ggcgcgaagt gcgccagaaa ggggccgaat
     6241 tcttcgccat acagcatcag ccgtacgtcg ttcggcgggt gggcacgcgc gcattcggcg
     6301 gcggcggcgc gaaaccaatc gtcgccgacc aagcgcgcca ctgcggggta gttggcgtgc
     6361 agtgcgtcga tgcagccttt catgaccgtg ttgcggtgca ccgcgaaccc gggctggcga
     6421 gccagtggcc cgtcgtggcc actgcggttg cgcaacgcat gcgcgaacgc atgctggaaa
     6481 gcgtgcagcg cggtgctcat gccggctccc gctcggcagc gcgcatcgcg tcgtgggcgc
     6541 gctggcgctc ggtcagcagt tgctcgaagg ccggcaggtt gccgtcgcgc tcgatcaacg
     6601 tgggtcgcgc gccgccgcgc gcgagcacgc gctcgtacag cgaccacacg cgctcggcca
     6661 ccggcgcgtc atgcgaatcg atccacaagc cgttgcccag cagcggatcg gcactgtgcc
     6721 ccgccagatg aatttcggcc acggcgtcga ggggaaaggc gtccagatac gcgacggcat
     6781 ccaaccccag gttgtgcgcc gacacggcga cgttgttcac gtccagcagc aaccggcagt
     6841 cggtgcggcg caccagctcg cgcagaaact cgggttcggt ccactcgtgg ccttcaagat
     6901 gcaggtagtg cgatgggttc tcgatggcga tggacatgcc cagagcgcgc tgaatgctat
     6961 caatatgatt gcaaaccgtg cgcagcgcct cgccggtgcg cggcagcggc agcagatcgg
     7021 gcaggtatgc gccggcccag cgcgaccagg ccaggtgctc ggacaccagc gtgggttgaa
     7081 agcggcgcac gaagcgcgcc agcgcctgaa cgcgctcgcc gtcgggcggc tcacacccgc
     7141 ccagcgacag cgacacgctg tgcaccgaca gcgggtgctc ggcgcgcacc gcctccaacc
     7201 aggccaggcg cgggccacct gcgaccaggt agttctcggg atgcacctcg aaccacacgc
     7261 ccggtgcgcg gcaggccagc gcctgttcaa agtgctcggg cttgaggccg atgccggcgg
     7321 tgagagaccg cgtcatggtg cgcgttgtcc gctggtcagg acttgatggg cgccagcgag
     7381 cccattccct tgggtgtctt gagggtggtg caagtgccgg tgggcacgtg cttccaggcg
     7441 ttgccttggt agtcggcctt ggaggtgccg gcgcacgtgg tgcccgggcc cgcggcgcag
     7501 tcgttcttgc ctgccatggc aacgccatag cacttttcca tcttcggctt gtcgccgcct
     7561 tggggcatgt ccttgcccat gggtgcggtt tgcgcctgta cggcgccggc aacgagggcg
     7621 ctgaggctga gggctgtcaa ggtggccggc agatttttca tggttagctc ctgaagtggt
     7681 gaatgaagac cgcagccagt ggctgacggt cctttgccag ttcgatcccg ctggcacggc
     7741 ggttacagtg caaacgcccc gcgcgcaaaa aaaacttttc cacccgcgtg taacccggac
     7801 acgtctgcag ccgaatggat ggtggtaacc cagatgtctt tgagcgatgt cgaaagccaa
     7861 ttgcgcagcc tgctgctgca agggctggcc ggtgacgaag ccgcctaccg gcagtttttg
     7921 cagcggctgg ccgcccacct gcgcgcattt ctggggcggc gcctgttcgg ctggcccgac
     7981 gacgtggagg acctggttca ggaatgcctg ctggtcatgc acaacaagcg ccacacctac
     8041 caaagcgatc agccgctgac ggcctgggtg catgccatcg cgcgccacaa gctgatcgac
     8101 gtgctgcgcg ctcgctcggg gcgagaggcg ctgcacgatc cgctggatga cgagatgccg
     8161 ctgttcgccg ccagcgatca cgacgcgcag gacgcgcggc gcgacctgat gggcttgctg
     8221 caaaccctgc cggacaagca gcgccgtccg atcgagtgcg tgaagatcga tggcctgtcg
     8281 gtcgcggaga ccgcgcggct gacgggcctg tcggaatcgg cggtgaaggt gggcattcac
     8341 cgcggcctga agtcgctggc ggcccgcctg ggtcacagct acagcgcgac aaggagcaaa
     8401 gcgtgaaaac ggatgatttg atcggcatgt tggccacgca ggccgggccg gtacgcgcgg
     8461 cgcgcgccgg gccgcggctt gcgcaggcca cagccggggg cctgggcggc gcgctggccg
     8521 ttctgctgct gggctacggg ctgcgcccag gtctggcgca ggacgcgcag ttgccgatgt
     8581 tctgggtcaa gctgctgatt ccagtctgcg ttgcgggcgc cggcggtgtg ctggcggcgc
     8641 gccttgggcg ccccggtgtg gcggcgcggg gcgcgtggtg gggtgtggtg gtgccagtcg
     8701 tggcaatctg gctcctggct gccgtgcaat gggtgggcgc agcacctgcc gaccggcccg
     8761 cgctgctgtg gggtcagacc tggcgcacgt gcatcggcag catcgggctg atcgccttgc
     8821 cggtgtttgt ggccgcactg gcagcgctgc gggatttggc gcccacgcac gccgcttggg
     8881 ccggcgctgc ggctggcgca ctggccggcg gctgcggtgc ggcggtgtac gcgctgcact
     8941 gcccggaact cacggcgccg tttctggcgg tttggtacgt ggccggcatg gcgctgccgg
     9001 tggcgctggg cgccgtgatc gggccgcgtg tgttgcgctg gtaggtgtgc gcagggtccg
     9061 tgacacacgc atgcctgcag cgcgtatcac tataataaat gtagctgcca gtgcttgtca
     9121 gtcgagcgct ggcagcgtaa aatagtctta acgccgcttg ccgccgagca agctgcccag
     9181 ggtgccgcgc agcagctcgc ggcccatgcg gcgcatctcg cccttgatga cgttctgcac
     9241 caggccgtcg
//
LOCUS       DAOMVO010000071         9147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1929_length_9147_cov_12.426969, whole genome shotgun sequence.
ACCESSION   DAOMVO010000071 DAOMVO010000000
VERSION     DAOMVO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_9147_cov_12.426969"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..499)
                     /locus_tag="PKV30_05195"
     CDS             complement(134..499)
                     /locus_tag="PKV30_05195"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRL29649.1"
                     /translation="MLVSHTGVHATCRGPRKLSDAHIKAIAATGGVMGIGYFEGAVCG
                     RDTAAIVRAIQHVATIAGVKHVALGSDFDGAVKTAFDTTGLALITQGLINAGFSDADI
                     ADVMGGNVLRLLQAALPPG"
     gene            complement(553..1602)
                     /locus_tag="PKV30_05200"
     CDS             complement(553..1602)
                     /locus_tag="PKV30_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRL29650.1"
                     /translation="MLHHLVLATRRLAPLLLTAALAACGSAPSLAPPITADPQAPKAG
                     ATFTDSLGIVFARVPAGEFLMGSDESPQALAQAFPHADPKRLTDLADEAPVHRVRITR
                     GFWLGAHEVTVGQFRRFLRESGYVPESVRDGTGGYGFNPNYDPSRTERGDLFEGRDPR
                     YSWESPGFPQTDRHPVVNVTWNDAVAMAKWLSQREGVTYRLPTEAEWEYAARAGTRTR
                     FPAGDDPLVLLKTANTFDRETALRWPRWRDQAGAGSDHYPFTAPVGSFAPNAFGLYDM
                     IGNAWEWCADWYGEDYYAQSPASDPTGPADGNVRVRRGGSWHSWPLYARVAFRNWNTP
                     ETRYVLVGFRLLRER"
     gene            complement(1691..2503)
                     /locus_tag="PKV30_05205"
     CDS             complement(1691..2503)
                     /locus_tag="PKV30_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL29651.1"
                     /translation="MLAIGFWPCRSFAQQGAAPRSWPRDSAVPGGVARIDLGPAAARP
                     QAWADAVPLLVLGSPSGWTALVGIALSAEPGTARIRVQGESGERELAYTIAPKRYTEQ
                     HLKVSPRTVDLSPEDLARHERERAHQQGVIATFSAPAAGEPHMRPPTPGRRSSSFGLR
                     RFFNGQPRNPHSGMDIAAPTGTPVVAPLAATVIDTGDYFFNGHTVWLNHGGGLLSMMC
                     HLSRIDCKVGDRLAVGQRLGTVGATGRVTGPHLHWTISLNRASVDPALFIDA"
     gene            2725..3237
                     /locus_tag="PKV30_05210"
     CDS             2725..3237
                     /locus_tag="PKV30_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HRL29652.1"
                     /translation="MALLQWVPELDTGIAEIDTQHKRIVDYINKLHELRQTHHREGLA
                     EVIAEMVDYTISHFAFEEALMESAGYMFSGPHKKVHDLFTRKVAEMQSRFDGGEDVTA
                     ELHGMLSRWLFNHIRNEDHAYIDSVQAYQRMAVSERDASKAQIKAEILKELAEERGRS
                     KGWLARLLGV"
     gene            complement(3268..3906)
                     /locus_tag="PKV30_05215"
     CDS             complement(3268..3906)
                     /locus_tag="PKV30_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL29653.1"
                     /translation="MNAAARLAPRALYEEVAERLRQRIYARELAPGDWIDELKIAEEL
                     GISRTPLREALKVLAAEGLVTMKVRRGAYVTETSAKDLADVYHLLELLETDAAAMVAQ
                     HASDADLQALAALHADLEAAAGDSTRFFALNEAFHLRVLALADNKWRLQMVQDLRKVM
                     KLHRQHSLQKAGRLQASLAEHAALMAALAARDGDRAASCMRQHFRSGLEAAS"
     gene            4105..6261
                     /gene="scpA"
                     /locus_tag="PKV30_05220"
     CDS             4105..6261
                     /gene="scpA"
                     /locus_tag="PKV30_05220"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRL29654.1"
                     /translation="MSTDSSSFKSATLADWQKAAQKSAPGGDVAALNWQTPDGITVKP
                     LYTAEDVAGLPYANTLPGFEPFLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKAL
                     AAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSVS
                     MTMNGAVLPILAGYIVAAEEQGVSQDQLAGTIQNDILKEFMVRNTYIYPPGPSMKIIG
                     DIIEYTALHMPKFNSISISGYHMQEAGANQALEMAFTLADGKEYVKTAIAKGMDVDAF
                     AGRLSFFWAVGMNFYLEIAKMRAARLLWCRIMKEFNPKNPKSLMLRTHSQTSGWSLTE
                     QDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHI
                     TNVVDPWAGSYMMEKLTQDMADAAWAIIEEVDAMGGMTKAVDSGWAKLKIEAAAAEKQ
                     ARIDSGRDVIVGVNKYKLGKEDAIDILDVDNVKVRESQIARLNNIKQKRDPAQVSQTL
                     AAITKIAEGEPGNLLAAAVDAIRARATVGEVSDAMERVFGRHRADTQKVTGVYAAAYD
                     SAEGWDKLKQEIAEFAEEEGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDIGSL
                     FQTPEECARQAVENDVHAVGVSTLAAGHKTLVPAILAELKKQGAGDIIVFVGGVIPRQ
                     DYDMLYEAGVKGIYGPGTPIPASAKDVLEQIRKARG"
     gene            6511..6720
                     /locus_tag="PKV30_05225"
                     /pseudo
     CDS             6511..6720
                     /locus_tag="PKV30_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            6997..8025
                     /gene="meaB"
                     /locus_tag="PKV30_05230"
     CDS             6997..8025
                     /gene="meaB"
                     /locus_tag="PKV30_05230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRL29655.1"
                     /translation="MEGGSALIAGITGAPGPAQRRAIAKTITLLESTRADHRAQADEL
                     LTALLPHTGQSLRIGISGVPGVGKSTFIETLGVYLIEKGHRVAVLAIDPSSTVSGGSI
                     LGDKTRMDRLSTLEAAYIRPSPSSGTLGGVAEKTRESLLVCEAAGYDVVLVETVGVGQ
                     SETAVSGMTDMFVLLQLPNAGDDLQAIKKGVMELADLVVINKADIDPAAATRAQAQIK
                     SSLRLLGLHGHPDHAAHDDQLWQPQVIQISGLRGDGIDTFWAAVTRFQTLQTANGRLA
                     ARRQQQAQAWMWERIDAGLKHAFRHHPGVRAVLQTTESEVRQGALPPSIAARRLLQLA
                     GLAQAAAC"
     gene            8033..8866
                     /locus_tag="PKV30_05235"
     CDS             8033..8866
                     /locus_tag="PKV30_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29656.1"
                     /translation="MSLTAQDLDRFARSPGWRATRLEVFDSAAGKVIVKGQRAPRHPA
                     RYHVLNLLARGVGLPLLQAAPMHGGKRAQAVEIDRLAQLRAAGTRVPEVLHVAPDHFV
                     MQWLGDQNLASLLQARHPHALALWREGANALVKLHASGQYLSQGFARNLIVDERTQPP
                     RLAGMIDFEDDPLEVMTLPEAQVRDWLSYLHSSLWILGLPPHQLDVTLDEFMVRESPD
                     VRALFLDACTRLAWLRLLPASRRLGRDVISLQAFADAAHRYRQRHRAAAPTPSEQPES
                     D"
     gene            8873..>9147
                     /locus_tag="PKV30_05240"
     CDS             8873..>9147
                     /locus_tag="PKV30_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL29657.1"
                     /translation="MKAILEQLENKRAAARLGGGQKRIEAQHNKGKLTARERIEVLLD
                     AGSFEEWDMFVEHRCADFGMDATHIPGDGVVTGYGTINGRLVFVFSQ"
BASE COUNT         1638 a         2916 c         3138 g         1455 t
ORIGIN      
        1 cggcctgtac tacgccatgt ggcgccagca gatcggcgag cgcgagagcg gggcgacaat
       61 cgaaaaacgg catcaaaacg ccgtccagcg cttgccagac gagcgcgagc agctatcaat
      121 ggtgtagcga acgtcagccg ggcggcagcg cggcctgcag caggcgcagc acgttgccgc
      181 ccatcacgtc ggcaatgtcg gcgtcgctga agccggcgtt gatcagccct tgcgtgatga
      241 gcgcgaggcc cgtggtgtcg aaggcggttt tcaccgcgcc atcgaagtcc gaacccagcg
      301 ccacatgctt gacgccagcg atggtggcca cgtgctggat cgcgcgcacg atggccgcgg
      361 tgtcgcggcc gcacacggcg ccttcgaaat agccgatgcc catcacgccg ccggtggcgg
      421 caatggcctt gatgtgcgca tcgctcagct tgcgcggccc gcggcaggtg gcatgcacgc
      481 cggtgtgcga gacgagcacc ggccgctgcc gttatcccct gggggaagcc gcgcagcggc
      541 tccgggcggc cgtcatctct cgcgcagcag cctgaagccg accagcacgt agcgcgtttc
      601 cggcgtgttc cagttgcgga aagccacgcg cgcatacagg ggccagctgt gccacgagcc
      661 gccacggcgc acgcgcacat tgccgtcggc cgggccggtc ggatcgctgg cgggcgattg
      721 cgcgtaatag tcttcgccgt accagtcggc acaccattcc catgcgttgc cgatcatgtc
      781 gtacaggccg aaggcgttgg gcgcaaagct gcccacgggc gcggtgaacg ggtagtggtc
      841 gctgccggcg ccggcctggt cgcgccagcg cggccagcgc agcgcggttt cgcggtcgaa
      901 ggtgttggcg gtcttgagca gcaccagcgg atcgtcaccg gcgggaaagc gcgtgcgcgt
      961 gccggcgcgg gcggcgtatt cccactcggc ttcggtcggc aggcggtagg tcacgccttc
     1021 gcgctggctg agccacttgg ccatggccac ggcgtcgttc caggtgacgt tgaccacggg
     1081 gtggcggtcg gtttgcggaa agcctgggct ctcccacgaa tagcgcggat cgcgcccctc
     1141 aaacaagtcg ccgcgctcgg tgcgcgaggg gtcgtagttg gggttgaagc cgtagccgcc
     1201 ggtgccgtcg cgcaccgatt cgggcacgta gcctgattcg cgcaggaagc ggcggaactg
     1261 cccgaccgtg acctcgtgcg cgcccagcca gaagccgcgc gtgatgcgca cgcggtgcac
     1321 cggcgcctcg tcggccaggt cggtcaggcg cttcgggtcg gcgtgcggaa aggcctgggc
     1381 cagcgcctgt ggggattcgt cgctgcccat caaaaattcg cctgcgggca cgcgggcgaa
     1441 gacgatgccc aggctgtcgg tgaaggtggc gccggccttg ggtgcctgcg ggtcggccgt
     1501 gatgggcggc gccagcgaag gcgcgctgcc gcaggcggca agcgccgcgg tcagcagcag
     1561 cggcgccagg cggcgggtgg ctaagacgag gtgatgcaac atggcggacg atgctcgcag
     1621 tggccggtgg ccgtgtcaac gctggcaaac accgaggcgc caggcgctgg gggatgaatc
     1681 gatcagccta tcacgcgtcg atgaacaggg ccgggtcgac cgacgcgcgg ttcaggctga
     1741 tggtccagtg caggtgcggg ccggtgacgc gcccggtggc gccgacggta cccaggcgct
     1801 ggccgacggc aagccggtcg ccgaccttgc agtcgatgcg gctcaggtgg cacatcatcg
     1861 acagcaggcc gccgccgtgg ttgagccaca ccgtgtggcc gttgaagaag tagtcgcccg
     1921 tgtcgatcac cgtggcggcc agcggcgcga cgaccggcgt gccggtgggc gcggcaatgt
     1981 ccatgccgct gtgcgggttg cgcggctggc cgttgaagaa gcgccgcaag ccaaacgagc
     2041 tggagcgccg cccgggcgtg ggtgggcgca tgtgcggctc gccggcggcc ggcgcgctga
     2101 aggtggcaat cacgccttgc tggtgcgcgc gttcgcgctc gtgccgcgcc aggtcttcgg
     2161 gcgacagatc gacggtgcgc ggcgacactt tgaggtgctg ctcggtgtag cgtttgggcg
     2221 cgatggtgta ggccagctcg cgctcgccgg actcgccttg cacgcgaatg cgcgccgtgc
     2281 cgggttcggc cgacagcgca atgccgacca gcgccgtcca gccgctcggg ctgcccagca
     2341 ccagcagcgg caccgcgtcg gcccacgcct gcgggcgcgc ggcggcgggc cccaggtcga
     2401 tgcgtgccac gccgccaggc acggccgaat cgcgcggcca gctgcggggc gccgcccctt
     2461 gctgcgcaaa cgaacggcac ggccagaaac cgatggccaa catgcccagc agccaggctc
     2521 ggcgacgttc tgacagatgc ggcttgaagg aaaaataaga cataaaaacg gccttggcgc
     2581 ttgctggtag tgcgtgagaa gctacgaata gtatagcgat ggtgtgctgc ggtcggtgac
     2641 tgcgcgctcg ttgatatgag tcaataagtt ctcgcgcaca tgaccggata ctgcgtgcat
     2701 tggcaagatt gagggtattt tttcatggct cttcttcaat gggtgccgga gctggacacc
     2761 ggcattgcag agatcgacac ccagcacaag cgcatcgtcg actacatcaa caagctgcac
     2821 gagctgcgcc agacgcacca ccgcgagggg ctggccgagg tgatcgcgga gatggtggac
     2881 tacaccattt cgcactttgc ctttgaagag gcgctgatgg aaagcgccgg ctacatgttc
     2941 agcggcccgc acaagaaggt gcacgacttg ttcacccgca aggtggccga aatgcaaagc
     3001 cgcttcgacg gtggcgagga cgtgacggcc gaactgcacg gcatgttgtc gcgctggctg
     3061 ttcaaccaca ttcgcaatga agaccacgcc tacatcgatt cggtgcaggc gtaccagcgc
     3121 atggcggtga gcgagcgcga tgcgtccaag gcgcagatca aggccgaaat actgaaagag
     3181 ctggccgagg agcgcggccg cagcaagggc tggctggcgc gcctgctggg cgtctgacgc
     3241 ggcggcggct ggcgggcgcc gcgccgctca actggcggct tccagcccgc tgcgaaagtg
     3301 ctggcgcatg cagctggctg cgcggtcgcc atcgcgcgcc gcgagtgccg ccatcagcgc
     3361 ggcatgctcg gccagcgagg cctgcagccg cccggccttt tgcagcgaat gctggcggtg
     3421 cagcttcatc accttgcgca aatcctgcac catctgcagc cgccatttgt tgtcggccag
     3481 tgccagcacg cgcaggtgaa acgcttcgtt caaggcaaaa aagcgcgttg agtcgccagc
     3541 ggcggcttcc aggtcggcat gcagcgcggc cagcgcctgc aaatccgcat cgctggcgtg
     3601 ctgggccacc atggccgcgg cgtcggtctc cagcagttcc agcaggtggt acacgtcggc
     3661 cagatccttg gcggacgttt cggtgacgta cgcgccgcgc cgcaccttca tggtcaccag
     3721 gccttcggcg gccagcacct tcagcgcttc gcgcagcggc gtgcggctga tgcccagttc
     3781 ttcggcaatc ttgagctcgt cgatccagtc acccggcgcc agttcgcgcg cgtaaatgcg
     3841 ctggcgcagg cgctcggcca cctcttcgta aagggcgcgc ggggcaaggc gagcggcagc
     3901 gttcatgggc gaaaaatttt cggcgattgt aagtcggtaa atggattttg aattaataat
     3961 tatggaacaa tagcggcatc ccacggtgtc tgcggcgcga gaggttgagg atcatcaaca
     4021 tgtccatgcg gcgcgccaca atgggcctgc ggcgcgcgct gcgggctgag cactgctgcc
     4081 ttatccatcc acacgatcca tgccatgagc accgattcat cctcgttcaa atccgcaacc
     4141 ttggccgatt ggcagaaagc cgcgcaaaag tccgcgcccg gcggcgatgt ggcagcgctc
     4201 aactggcaaa cgcccgacgg catcactgtc aagccgctgt acacggcaga ggacgtggcc
     4261 ggcctgccgt acgccaacac gttgcccggt ttcgagccct tcctgcgcgg cccgcaggcc
     4321 accatgtacg cggtgcggcc atggacgatt cgtcagtacg cgggcttttc gaccgccgag
     4381 gagtccaacg ccttttatcg caaggcgttg gcggcgggcg ggcagggcgt gtcggtggcg
     4441 tttgacttgg cgacgcaccg gggctatgac tcggaccatc cccgcgtcac aggcgacgtg
     4501 ggcaaggccg gtgtggccat cgacagcgta gaggacatga agatcctgtt cgacggcatt
     4561 ccgctggaca aggtgagcgt gtcgatgacc atgaacggcg ccgtgctgcc catcctggcc
     4621 ggctacatcg tggcggcgga agagcagggg gtcagccagg accagttggc gggcacgatc
     4681 cagaacgaca tcctcaagga gttcatggtt cgcaacacct acatctaccc gcccgggccg
     4741 tcgatgaaga tcattggcga catcatcgag tacacggccc tgcacatgcc gaagttcaat
     4801 tcgatcagca tcagcggcta ccacatgcag gaagccgggg cgaaccaggc gctggagatg
     4861 gccttcacgc ttgccgatgg caaggagtac gtgaagaccg ccatcgccaa gggcatggat
     4921 gtggatgcgt ttgccgggcg actcagtttc ttctgggccg tgggcatgaa cttctatctg
     4981 gagatcgcca agatgcgcgc cgcgcgcctc ttgtggtgcc ggatcatgaa ggagttcaac
     5041 cccaagaacc cgaagtcgct gatgctgcgc acgcattcgc agacatcggg ttggtcgctg
     5101 accgagcagg atccgtacaa caacgtggtg cgcaccacca tcgaggcgat ggcggcggta
     5161 tttggcggca cgcaaagtct gcacaccaac gcgctcgacg aagcgattgc gctgccgacg
     5221 gagttcagcg cccgcatcgc acgcaacacg cagctcatca tccaggaaga aacgcacatc
     5281 accaacgtcg tcgatccctg ggccggcagc tacatgatgg aaaagctgac gcaggacatg
     5341 gccgatgccg cctgggccat catcgaagag gttgatgcca tgggcggcat gaccaaggcg
     5401 gtggacagcg gttgggccaa gctgaaaatc gaagccgcgg cggctgaaaa gcaggcccgc
     5461 atcgattcgg gccgcgacgt gatcgtcggc gtcaacaaat acaagctggg caaggaagac
     5521 gccatcgaca tcctggatgt cgacaacgtc aaggtgcgtg aatcgcagat tgcgcggttg
     5581 aacaatatca agcaaaaacg cgatccagcg caggtcagtc aaacgctggc agctattaca
     5641 aaaatagcag aaggcgaacc cggcaacctg ctggccgccg ccgtggacgc catccgcgcc
     5701 cgcgccaccg tgggcgaagt gagcgatgcg atggagcggg tcttcgggcg ccaccgcgcc
     5761 gacacgcaaa aggtgaccgg tgtgtacgca gccgcctacg actcggccga aggctgggac
     5821 aagctcaagc aagagatcgc cgaattcgcc gaagaagaag gccgccgccc gcgcgtgatg
     5881 atcgccaagc tgggccagga cggccacgac cgcggcgcca aggtggtggc cacggcgttt
     5941 gccgacttgg ggtttgacgt ggacatcggc agcctgtttc agacgccaga ggaatgcgcg
     6001 cgccaggcgg tggagaacga cgtgcacgcg gtgggtgtgt caacgctcgc cgcagggcac
     6061 aagacgctgg tgccggccat cctcgctgaa ctcaagaaac agggcgccgg cgacatcatc
     6121 gtcttcgtcg gcggcgtgat tccccggcag gactacgaca tgttgtacga ggcgggcgtc
     6181 aagggcattt acggcccagg cacgccgatt ccggccagtg cgaaggatgt gctggagcag
     6241 atcaggaagg cgcggggctg agcagattgc gcaacgtgat cgtgttcacc gcttcacccc
     6301 ttcaccggat gcctcatgga actgaatgcc ttatgaccac cgttcgggct gagcttgtcg
     6361 aagccgtgcg cgccgctcca tgcagccgtt gcccccccac cccgttcgtc ctgaacttgt
     6421 cgaaggacag gccgaccacc tccaatctga cgccaagcct cggcccgttc gcactgagcc
     6481 tgtcgaaggg cgagtcagcc tactgaccgc atgaagccct ttcacgtcta cctgttgcgc
     6541 tgcgccgatg actcgtacta ctgcgggcac acagacgatt tggtcgctcg cctggagcag
     6601 caccacatcg gcgcgggcgg ttacacggcc gcgcgtcgtc ccgtggaggt agtgtggcac
     6661 ggtgagttcg aatcgcgcgc cgaggcgctg gcgttcgagc agcaggtaaa gggttggtcg
     6721 cgcgccaaga aggaggcgtt gattgccggg gattgggggc gggtgagatt gctggcgcgg
     6781 gcaagaggcg caggggattc agcgaagccg ctcacgactg gcccttcgac aagctcaggg
     6841 cgaacggggg ggggtggcgt gctggccgat gggagtagac cggccttttc agcacagcag
     6901 agtgcaggcg tttcggcagg ctcagggcga gcggatgtgg gggcggaacc cctcgatcgt
     6961 gtttccccag gcccttctac aggctcaggg cgagcggtgg agggagggag cgccctgatt
     7021 gccggaatca ccggtgcgcc gggccccgcc cagcgccgcg ccatcgccaa aaccatcacc
     7081 ctgctggagt ccacccgcgc cgaccaccgc gctcaggccg atgaattgct gaccgcgctg
     7141 ctgccccaca cgggccaatc tctgcgcatc ggcatcagcg gcgtgccggg tgtgggcaaa
     7201 agcaccttca tcgagacgct cggcgtctac ctgatcgaaa aagggcaccg tgtggcggtg
     7261 ctggcgatcg acccttccag caccgtgtcg ggcggctcca tcctgggcga caagacgcgc
     7321 atggatcggc tgtcaacgct ggaagccgcc tacatccgcc ccagccccag cagcggcacg
     7381 ctgggcggcg tggccgagaa gacgcgcgag agtctgctgg tgtgcgaagc cgccggctac
     7441 gacgtggtgc tggtcgaaac ggtgggcgtg ggccagtccg aaaccgcggt cagcggcatg
     7501 accgacatgt tcgtgctgct gcaactgccc aacgccggcg acgatctgca ggccatcaag
     7561 aagggcgtga tggagttggc cgatctggtg gtcatcaaca aggccgacat cgaccccgcc
     7621 gccgccacgc gcgcccaggc gcagatcaag tcgtcgctgc gcctgctggg cctgcacggc
     7681 caccccgacc acgcggcgca cgacgatcag ctctggcagc cgcaggtgat ccagatcagc
     7741 ggcttgcgcg gcgatggcat cgacacattc tgggcggctg tgacgcgttt tcagaccttg
     7801 caaacggcca acgggcggct ggccgctcga cggcagcagc aggcccaggc gtggatgtgg
     7861 gagcgcatcg atgcgggtct gaaacatgct tttcgccacc accccggcgt gcgcgcggtg
     7921 ctgcaaacga ccgagagcga agtgcgccag ggcgcgctgc cgccttccat cgccgctcgg
     7981 cgcttgctgc aactcgcggg gctggctcag gccgccgcct gctgatgccg ccatgagctt
     8041 gaccgcacag gatctggacc gattcgcccg ctcgcccggc tggcgcgcca cgcggctgga
     8101 ggtgttcgac agcgctgcgg gcaaggtcat cgtcaagggc cagcgcgcgc cacgccatcc
     8161 ggcccgctac cacgtgttga acctgttggc gcggggcgtc ggtttgccct tgctgcaagc
     8221 cgcacccatg cacggcggca agcgggcaca agcggtcgaa atcgatcgcc tggcgcaact
     8281 gcgcgccgcc ggcacgcgcg tgcccgaggt gttgcacgtg gcgcccgacc atttcgtcat
     8341 gcagtggctg ggcgaccaga acctggccag cttgctgcag gcacgccatc cgcacgcgct
     8401 cgcgctgtgg cgcgagggcg cgaacgcgct ggtgaaactc catgcgtcgg gccaatacct
     8461 gagccagggc tttgcacgca acctgatcgt tgatgagcgc acacagccgc cgcgcctggc
     8521 aggcatgatc gacttcgagg acgatccgct cgaagtgatg acgctgcccg aagcgcaggt
     8581 gcgcgactgg ctgagctatt tgcacagcag cctgtggatc ctgggcctgc cgccccatca
     8641 gcttgatgtc acgctggacg agttcatggt ccgcgaatcc cctgatgtgc gcgccttgtt
     8701 tctggacgcc tgcacccggc tggcctggct gcgcctgttg cccgcgagcc ggcgcttggg
     8761 ccgcgacgtg atttcgctgc aggcctttgc cgacgccgcc caccgctacc ggcagcgcca
     8821 ccgcgctgcc gcccccaccc catctgagca acctgaaagt gattgaaatc cgatgaaagc
     8881 cattcttgaa caactggaaa acaagcgcgc ggccgcccgc ctgggcggcg gccagaagcg
     8941 cattgaagcg cagcacaaca agggcaagct gacggcgcgc gagcgcatcg aggtgctgct
     9001 cgatgccggc agcttcgaag agtgggacat gttcgtcgag caccgctgtg ccgacttcgg
     9061 catggacgcc acgcacattc cgggcgatgg cgtggtcacc ggctacggca ccatcaacgg
     9121 ccgcctggtg ttcgtcttca gccagga
//
LOCUS       DAOMVO010000072         9137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1932_length_9137_cov_17.390002, whole genome shotgun sequence.
ACCESSION   DAOMVO010000072 DAOMVO010000000
VERSION     DAOMVO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_9137_cov_17.390002"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKV30_05245"
     CDS             complement(<1..233)
                     /locus_tag="PKV30_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRL29658.1"
                     /translation="MKSRAAVAFEAGKPLQIVEIDVAPPKAGEVLIKITHTGVCHTDA
                     FTLSGDDPEGLFPAVLGHEGAGIVVEVGEGVTSV"
     gene            complement(252..551)
                     /locus_tag="PKV30_05250"
     CDS             complement(252..551)
                     /locus_tag="PKV30_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRL29659.1"
                     /translation="MPHSPEDKKRAVTRLRRIRGQAEALERSIAEGADCGPVLQQLAA
                     MRGAVNGLMADVLEGHLRESFGHADCAVDSLDDPEHVRMHEAIDEISSLVRSYLK"
     gene            646..1296
                     /locus_tag="PKV30_05255"
     CDS             646..1296
                     /locus_tag="PKV30_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29660.1"
                     /translation="MSLQSLARKLLVLSAAALPLVAHAGRPMMVDDAGVNDAGAGHLE
                     TWFERGPGGHRAWTAAPAYAPIEGLELGAAVSRDTTERSTALRLQGKLQSTRPTEDAC
                     GHAAVLGIAHERPKAVNARWFNLVMSCPVAPGNVHLNLGATQTARKSAKAFIGAAWEQ
                     DLGWASGHVEWIAAQSAQPIVNLGLRREIGKGLQLDGSVGRQAGRTLFSVGLKQQF"
     gene            complement(1351..1935)
                     /locus_tag="PKV30_05260"
     CDS             complement(1351..1935)
                     /locus_tag="PKV30_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRL29661.1"
                     /translation="MKHRSLEIIRDEHSSLAAMLQSMRMLVDRGPQGNPKNFFDVLRA
                     MLFYIDEFPERLHHPKETELLFPRVAAAAPDVGVAVARLDRDHEYTEHAVREIQHLLL
                     GWELLGEARRQVFVDAFTKYVNLYLAHMRLEEQEILPAAEKYLSEADWKLLDAAFEEN
                     CDPLTGKYKPDPVYEQLFSRIVREAPAPIGLGGG"
     gene            complement(2089..4512)
                     /locus_tag="PKV30_05265"
     CDS             complement(2089..4512)
                     /locus_tag="PKV30_05265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRL29662.1"
                     /translation="MNDTSEQTLDLGIGGMTCASCVARVEKALKKVPGVQNANVNLAT
                     ESARVTYAGGEDMAPALRRAVRDAGYEPRAADAALDAPDAGPWAGFAPIAIGLLLCAP
                     LVLPMLLMPFGIHWMPPAWVQFALATPVQFWLGARFYKAAWGALKAGSGNMELLVAIG
                     TTAAWLLSMWLWLFSGHGEHAHLYFESAAVVITLVRLGKWLEERAKRQTTAAIRALHA
                     LRPSVAHLIDWDGAEKDIPVDELRAGDKLAVRPGERVPADGTVTEGATQVDESMLTGE
                     PLPVAKAVGDALTGGSINGDGRVLMTVTAAGSESVLAHIIRLVQDAQAVKAPVQRLVD
                     QVSAVFVPVVIAIALATLAGWWLAGVGAETAVIRAVAVLVIACPCALGLATPTAIMAG
                     TGVAAKFGILIKDAEALEVAHKVDTVAFDKTGTLTLGQPKLLALVPAPGVDEAAALRA
                     AASLQSGSEHPLARAVLADARDKGVAIDAPQDVRAVPGRGTEGRVAGHEVLIGSARWM
                     NELGVDLKPFEAAAHDWQAQGATISIVAIRTANEQTPREQNAAALREQNHAALREHNH
                     AALRAVALLAFGDEPKPGARAAIDALHARGVRAVMISGDNRGAAEAMARRLGLNPDAG
                     EVMAEVLPADKAAAVQALRAGSMVFPPLPGGGEGWGEGGGPSHVTAPELAAHPSPPPS
                     PAGGRGSKTHTVAMVGDGVNDAPALAAADVGMAMGSGTDVAMHAAGITLMRGDVALVP
                     AALDISARTVAKIRQNLFWAFIYNVAGIPLAALGYLNPVMAGAAMALSSVSVMSNALL
                     LKRWRPRDE"
     gene            4705..4902
                     /locus_tag="PKV30_05270"
     CDS             4705..4902
                     /locus_tag="PKV30_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRL29663.1"
                     /translation="MNQPFTVTGMTCGHCEMAVRRAVKSVDPQAEVKIDRADNRVEVQ
                     SDQPREAIAAAIREEGYPVAE"
     gene            4899..5318
                     /gene="cueR"
                     /locus_tag="PKV30_05275"
     CDS             4899..5318
                     /gene="cueR"
                     /locus_tag="PKV30_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HRL29664.1"
                     /translation="MSTSAPDSPVNIGAAARASGVSAKMVRHYESLGLLGDITRTDSG
                     YRQYSAADVHTLRFIKRARDLGFSMAEIAELVSLWHDRARASADVKRIAKAHVADLEN
                     RIAALQDMRSTLQNLLQHCHGDARPQCPILDRLAGGS"
     gene            5482..5673
                     /locus_tag="PKV30_05280"
                     /pseudo
     CDS             5482..5673
                     /locus_tag="PKV30_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
     gene            5836..6363
                     /locus_tag="PKV30_05285"
     CDS             5836..6363
                     /locus_tag="PKV30_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRL29665.1"
                     /translation="MTQHPKRLFLAGLLAASLGSVAIAQNAPQATAAQPAAGEASGRA
                     RMDPAQRQQRMAEHHAKRQAELKAQLKLTPAQEGAWNQYTSAMQPPAKGPRMDRAEFD
                     KLTTPQRIDRMQQRSAERQAQMKQRGDAVKAFYAQLTPEQQKVYDERAMRGGRGEGQR
                     GGGRHGHHGMGPGPR"
     gene            6572..7429
                     /locus_tag="PKV30_05290"
     CDS             6572..7429
                     /locus_tag="PKV30_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRL29666.1"
                     /translation="MAPAPPPLPPRPARGTLKLPGVRQVELTAPLTWLALGWRDVWRI
                     GLPSLMHGVVLAAFGALLVFIARDRFWLLAGAFSGFLVVAPVLATSLYALSRALERGE
                     PTGWRTIGQTWTRWQYSRFAVHGGYWSLVRFGLLLSLAGTGWVLTSAALITALAPAPV
                     NTPMDFARHVVLAPGGYLFELWLTMGALMAAPLFASSVVSMPLLLDRRVDVLQAVLTS
                     WQTVLTNPGALALWAALIMALTGLGMLTALLGLIVVVPWLGHASWHAYRALVDASSLP
                     ERLPPEGQR"
     gene            7429..7638
                     /locus_tag="PKV30_05295"
     CDS             7429..7638
                     /locus_tag="PKV30_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HRL29667.1"
                     /translation="MFGFNEEQIAWFGLTVGVGAFMLYMLFIIGHLAWESKAGKFGTF
                     VIFLGLAFGMVGFVAKGIIEWWMTR"
     gene            8165..8935
                     /gene="minC"
                     /locus_tag="PKV30_05300"
     CDS             8165..8935
                     /gene="minC"
                     /locus_tag="PKV30_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRL29668.1"
                     /translation="MSVALAGRSPTTFEIKSASLPLLALRLKSADLDQLADELQAQYG
                     EMPDFFEDDAVVLDLCALPEEAADASIDFQRLGELLHERRLRLLAVRGGNAAHSAAAT
                     DIGLLLAPDARVQAPPAPAEPVPTAKAPVDAAAAHNLPAPGALVIDKPLRSGQQVYAR
                     GRDLVVMAMVNAGAEVIADGHIHVYAPLRGRAIAGARGWPEARILAREMRPELVSIAG
                     VYRASDEPLPPEVWGRPASVQLASSEAGDKLIFTPIAD"
     gene            8993..>9137
                     /locus_tag="PKV30_05305"
     CDS             8993..>9137
                     /locus_tag="PKV30_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL29669.1"
                     /translation="MAKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLR
                     NLDL"
BASE COUNT         1444 a         3251 c         2969 g         1473 t
ORIGIN      
        1 acgctggtca cgccttcgcc cacttccacc acgatgccgg cgccttcgtg gcccagcacg
       61 gcggggaaca ggccctcggg gtcgtcgccg ctcagcgtga aggcgtcggt gtggcacacg
      121 ccggtgtggg tgatcttgat cagcacctcg ccggctttgg gcggcgcgac gtcgatttcg
      181 acaatctgca agggcttgcc ggcctcgaag gcgacggcgg cgcgggattt catgggggat
      241 ctccttaggg tctatttcag atacgagcgc accagcgacg agatttcgtc gatcgcctcg
      301 tgcatgcgga cgtgttcggg gtcgtccagg ctgtccacgg cgcaatcggc atggccgaac
      361 gactcgcgca aatggccttc cagcacgtcg gccatcagcc cgttgacggc gccgcgcatc
      421 gccgccagct gctgcagcac cgggccgcag tcggcgcctt cggcgatgct gcgctccagc
      481 gcctcggcct ggccgcggat gcggcgcagg cgggtgacgg cgcgcttctt gtcttcaggt
      541 gaatggggca tggcggatac tcaggggcag tttataaccc agactgcgcg gcagttcaat
      601 tcaatgtcac acgacctgaa ggtggcgcca agcacgatga ccgccatgtc gcttcaatct
      661 ctcgcgcgca agctgctggt gctgtccgcc gccgcgctgc ccctggtcgc ccacgccggg
      721 cggccgatga tggtcgatga cgctggcgtc aacgacgcag gcgccggcca cctcgaaacc
      781 tggttcgagc gcggccccgg cggccaccgc gcctggacgg ccgcgcccgc ctatgccccc
      841 atcgaggggc tggagctggg cgccgccgtc tcgcgcgaca ccaccgagcg cagcaccgcg
      901 ctgcgcctgc aaggcaagct gcaatccacc cggccgaccg aagacgcctg cggccacgcc
      961 gccgtgctgg gcatcgcgca cgagcgcccc aaggccgtca acgcgcgctg gttcaacctg
     1021 gtcatgagtt gccccgtcgc gcccggcaac gtgcacctga acctgggcgc cacgcagacc
     1081 gcccgcaagt ctgcgaaagc cttcatcggc gcggcgtggg agcaagattt gggctgggcc
     1141 agcggccacg tcgaatggat cgcggcgcaa agcgcccaac cgatcgtcaa cctgggcctg
     1201 cggcgcgaga tcggcaaggg gctgcagctc gacggttcgg tcggccggca ggccggccgc
     1261 acgctgttca gcgtggggct gaagcagcag ttctgagcac caaaaaagcc gcgacatcgc
     1321 ggcttttctg gagcggagcg ccggcggctt tcagccccca cccagcccga tcggcgcagg
     1381 cgcctcgcgc acgatgcggc tgaacaattg ctcgtacacc gggtcgggct tgtacttgcc
     1441 ggtgagcggg tcgcagttct cctcgaacgc ggcatccagc agcttccagt cggcctcgct
     1501 caggtacttt tcggcggcgg gcaggatttc ctgctcttcc agccgcatgt gggccaggta
     1561 caggttgacg tacttggtga aggcgtcaac gaacacctgc cgccgcgcct cgcccagcag
     1621 ctcccacccc agcagcaggt gctggatctc gcgcaccgcg tgctcggtgt attcgtggtc
     1681 gcggtccagc cgcgccacgg ccacgccgac gtcgggcgcg gccgccgcca cgcggggaaa
     1741 cagcagctcg gtctctttcg ggtggtgcag ccgctcggga aactcgtcga tgtagaacaa
     1801 catggcgcgc agcacgtcga agaagttctt cgggttgccc tgcgggccgc gatccaccag
     1861 catgcgcatc gattgcagca tggccgccag cgacgaatgc tcgtcgcgaa tgatctccag
     1921 gctccggtgt ttcatggcgc gtgtctcctg ttctgccgcg ttcgagttta gagcggcgca
     1981 ccagaatgac gcatgacatg tgtcaagttg gcacggcgtg gcgtgatgtc gaatcactac
     2041 gttttcaata gctgcctgcg ctttctggac aagcgctgga gccttatttc attcatcccg
     2101 cgggcgccag cgcttgagca acagcgcgtt gctcatcacg ctgaccgaac tgagcgccat
     2161 ggcggcgccg gccatcaccg ggttcaggta gccgagcgcc gccagcggaa tgcccgccac
     2221 gttgtagatg aaggcccaga acaggttctg ccggatcttg gccaccgtgc gcgccgagat
     2281 gtcgagcgcc gccggcacca gcgccacgtc gccgcgcatc agcgtgatgc cggcggcgtg
     2341 catggcgacg tcggtgccgc tgcccatggc catgccgacg tcagccgcgg ccagcgcggg
     2401 cgcgtcgttg acgccgtcgc cgaccatggc gacggtgtgg gtcttgctcc ctctcccgcc
     2461 tgcgggagag ggtgggggtg aggggtgcgc cgccaactcg ggcgcagtga cgtgggaagg
     2521 ccccccaccc tcaccccagc cctctccccc gcccgggaga ggtggaaaga ccatcgagcc
     2581 cgcgcgcagc gcctgcaccg ccgccgcctt gtccgccggc agcacctcgg ccatcacctc
     2641 gcccgcgtcc gggttcaagc ccagccgccg cgccatggcc tcggcggcgc cccggttgtc
     2701 gccgctgatc atcacggcgc gcacgccgcg cgcgtgcagc gcgtcgatgg ccgcgcgcgc
     2761 gccgggcttg ggctcgtcgc caaaggccag cagggccacg gcgcgcagcg ccgcatggtt
     2821 gtgttcgcgc agcgccgcat ggttctgttc gcgcagcgcc gcagcgttct gttcgcgtgg
     2881 cgtctgctca ttggcagtgc gtatggcaac tattgaaatc gtagcgcctt gcgcttgcca
     2941 gtcgtgcgct gcagcctcaa aaggcttcaa atccacgccc agctcgttca tccagcgcgc
     3001 gctgccgatc agcacctcgt ggcccgccac gcggccttcg gtgccgcgcc cgggcacggc
     3061 gcgcacgtct tgcggggcgt cgatggcaac gcctttgtcg cgcgcatcgg ccagcacggc
     3121 gcgcgccagc gggtgttcgc tgcccgactg caaactcgcc gccgcccgca gcgccgccgc
     3181 ctcgtccacg cccggcgcgg gcaccagcgc cagcagtttc ggctggccga gcgtcagcgt
     3241 gccggtcttg tcgaaggcca cggtgtcgac cttatgcgcc acctccagcg cctcggcgtc
     3301 cttgatgaga atgccgaact tcgccgccac gccggtgccg gccatgatgg ccgtcggcgt
     3361 ggccagcccc agcgcgcacg ggcaggcaat caccagcacg gccacggcgc ggatgacggc
     3421 cgtctcggcg cccacgcctg ccagccacca gccggccaag gtcgccagcg cgatggcgat
     3481 caccaccggc acgaagaccg cgctcacctg gtccaccagc cgctgcaccg gcgccttcac
     3541 ggcctgcgcg tcttgcacca ggcggatgat gtgcgccagc acgctctcgc tgccggccgc
     3601 cgtcaccgtc atcagcacgc ggccgtcgcc gttgattgag ccgccggtca gcgcatcgcc
     3661 caccgccttg gccaccggca gcggctcgcc ggtcaacatg ctttcatcga cctgcgtggc
     3721 gccttcggtc accgtgccat cggcgggcac gcgctcgccg gggcgcacgg cgagcttgtc
     3781 gcctgcgcgc agctcatcga ccgggatgtc cttctcagcg ccatcccagt cgatcaggtg
     3841 cgcaacggaa ggccgcagcg catgcagcgc gcggatggcg gcggtggtct ggcgcttggc
     3901 gcgctcttcc agccacttgc ccaggcgcac cagggtgatg acgacggccg cgctttcaaa
     3961 gtacaggtgc gcgtgctcgc cgtggccgga aaacagccac agccacatcg acagcagcca
     4021 cgccgccgtg gtgccgatgg cgaccagcag ttccatattg ccgctgccgg ccttcaacgc
     4081 gccccaggcc gccttgtaaa agcgcgcgcc gagccagaac tgcaccggcg tggccagcgc
     4141 aaactgcacc cacgcgggcg gcatccagtg aatgccgaag ggcatcagca gcatcggcaa
     4201 caccagcggc gcgcacagca gcaagccgat ggcgatgggc gcaaagccgg cccatggccc
     4261 ggcgtcgggc gcatccagcg ccgcatcggc ggcgcgcggc tcgtagccgg cgtcgcgcac
     4321 ggcccggcgc agcgcgggcg ccatgtcctc gccgccggcg taggtcacgc gcgccgattc
     4381 ggtcgccagg ttgacgttgg cgttctgcac gccgggcacc ttcttcagcg ccttctcgac
     4441 gcgcgccacg cagctggcgc aggtcatgcc gccaatgccc aggtccaggg tttgctcgct
     4501 tgtatcgttc atggacgcag tgtgacgcct gacaccgtgg caaggtcaag cgctgtagat
     4561 tttcatggtc aaaaaatgcc gccagcgcac gtcagacaag cgcaagcagc tatcaattta
     4621 atatattcct gttgagccgc ttgactttgc caccatgtga aggttcatca tcaccgcatc
     4681 gtccaaccca tcaggagact tgttatgaac cagcccttca ccgtcaccgg catgacctgc
     4741 ggccactgcg aaatggcggt gcgccgtgcc gtgaaatcgg tcgatccgca ggccgaagtc
     4801 aagatcgacc gcgccgacaa ccgggtcgag gtgcagtccg accagccgcg cgaagccatc
     4861 gccgccgcca ttcgcgaaga aggctatcca gtcgccgaat gagcacgtcc gcacctgaca
     4921 gcccggtcaa catcggcgcc gccgcgcgcg cctcgggcgt gtcggccaag atggtgcggc
     4981 actacgaatc gctcggcctg ctgggcgaca tcacgcgcac cgacagcggc taccgccagt
     5041 acagcgcggc cgacgtgcac acgctgcgct tcatcaagcg tgcgcgcgac ttgggctttt
     5101 cgatggcaga gatcgccgaa ctggtcagcc tgtggcacga ccgcgcacgc gccagcgccg
     5161 acgtcaagcg catcgccaag gcgcatgtgg ccgaccttga aaaccgcatc gccgccttgc
     5221 aggacatgcg cagcacgcta caaaatctgt tgcagcactg ccatggcgac gcgcgcccgc
     5281 agtgcccgat cctggatcgg ctggccggcg gctcttaaac aaaaaatccc cataccgccg
     5341 cgccgccttg gtgcgacgat cagcgctttg tatacaaacg tccgctttct ggcgcggccg
     5401 gcctgtgcgc aacgctgttg aggtgacgcc agaccggttc cagacaaggc tcaccacaag
     5461 atgtcgtccg tcacgctgaa gaacatggct gccatcgtca aggcgtacca cgacaccggc
     5521 tttaccggct acgtgcggcc cgaccatggc cgcatgatct ggggcgagca gggcaaggcg
     5581 ggctacggcc tgtacgaccg cgccttgggt gccgtgtacc tgaatggttt gtgggaagcg
     5641 atcgacaaat tcgccagtca gcccgccgcc tgatggcccg cagctttttc attcaacttt
     5701 acaaagcctc atcgcccagc caagggccgg atacacgcgc ggcggacact gctgccaagc
     5761 cagcagggca acccaacccg ctggccgagc aagacctgtg aaccgagtct ctctctgcca
     5821 tgaaaggaga acaccatgac tcaacatccg aaacgcctgt tcctcgccgg cctgctggcg
     5881 gcatcgctgg gcagcgtcgc catcgcgcag aacgcaccgc aagccaccgc tgcccagccg
     5941 gccgctggtg aagcttcggg ccgcgcccgc atggacccgg cccagcgcca gcagcgcatg
     6001 gccgagcacc acgccaagcg ccaggccgaa ctgaaagccc aactgaagct gaccccggcg
     6061 caggaaggcg cctggaacca gtacacctcc gccatgcagc cgccggccaa gggcccgcgc
     6121 atggatcgcg ccgagttcga caagctgacc acgccgcagc gcatcgaccg catgcagcag
     6181 cgctcggccg agcgccaggc gcagatgaag cagcgcggcg acgccgtgaa ggccttctac
     6241 gcccagctca cgcccgagca gcaaaaggtg tacgacgagc gcgccatgcg cggcgggcgc
     6301 ggtgagggcc agcgcggcgg gggtcgccac ggccaccacg gcatgggccc cggcccacgc
     6361 tgacctctct cgcagccacg gcgccgcgcc ccacccgcaa cgcgcctccc cgccagcagc
     6421 cggcttttgc cgctgctggc ttttttatgt caaaacagcc tctggcgctt gtctagcatg
     6481 cgcaagcagc tatacttttt gaattaaccg gtcacagcgc cgccgctggt gcgatggctg
     6541 aattgaccgt aagctgctgc ccgtgcccca catcgccccc gctcccccac cgctgccgcc
     6601 gcgccctgcg cgcggcaccc tgaaactgcc gggcgtgcgc caggtcgagc tgacagcgcc
     6661 cctcacctgg ctggcgctcg gctggcgcga tgtgtggcgc atcggcctgc ccagcctgat
     6721 gcacggcgtg gtgctggcgg cgtttggtgc gctgctggtg ttcatcgcgc gtgatcgctt
     6781 ttggctgctg gcgggcgcct tctccggttt tctggtggtg gcgccggtgc tggccaccag
     6841 cctgtacgcc ttgagccgcg cgctggagcg cggcgagccg accggctggc gcaccatcgg
     6901 ccagacctgg acgcgctggc aatactcgcg ctttgccgtg cacggcggct actggagcct
     6961 ggtgcgcttt ggcctcttgc tgtcgctggc cggcaccggc tgggtgctga cctcggccgc
     7021 tctgatcacg gcactggcgc cggcgccggt caacacgccg atggatttcg cccgccacgt
     7081 ggtgctggcg cccggcggct acctgttcga gctgtggctg accatgggcg cgctgatggc
     7141 ggcgccgctg tttgcgtcca gcgtggtcag catgccgctc ttgctggatc gccgtgtcga
     7201 cgtgctgcag gccgtgctga ccagctggca gacggtgctg accaaccccg gcgcgctggc
     7261 gctgtgggcc gcgctcatca tggcgctgac ggggctgggc atgctgaccg cgctgctggg
     7321 gctgatcgtc gtcgtgccct ggctcgggca cgccagctgg cacgcgtacc gggcgctggt
     7381 ggatgccagc agcctgcccg agcgcctgcc gccggagggg cagcgctgat gttcggcttc
     7441 aacgaagagc agatcgcctg gttcggcctc acggtcggcg tgggcgcgtt catgctctac
     7501 atgctgttca tcatcggcca cctggcgtgg gaatccaagg ccggcaagtt cggcaccttc
     7561 gtgatctttc tcggcctggc cttcggcatg gtgggcttcg tggccaaggg gatcatcgag
     7621 tggtggatga cgagataaag ctccccctgt gacgcctgcg gcgtcttccc ccggaggggg
     7681 acggcgccag tggccggggg ggaccccggc cacggcaccc cgatgtggcc tgctccgcgg
     7741 ccttttgagc cttgacttcg gatgcctcgc catcgtcgga cgttctttcg cttcaactaa
     7801 ccctgtgaat aacgctggca caagtgggca cttatccaca gccgcacgac agtggctgaa
     7861 cttgtccccg ttgcagcgcg gcggccccgg ttgctttcac acaggcgcag acgccagcca
     7921 agtgcttgtc gatgctgggc atttgcggct tgtccacaaa aacgcagggc acctactacg
     7981 actactactt tctataaaag aaaaagaaga cagatagcgc agccaaaacg ctgcccaaac
     8041 ccggctgcgc cggcttgtta cagcgccgtg ccggcgcagg cctttttgcg ccgaggcgac
     8101 tgttaagatc ggccagcttt ttccgccgcc cttcggcggc gtttaccgag acgctttcgc
     8161 gcccatgtcc gttgccctgg ccggccgcag ccccaccact ttcgagatca agagcgccag
     8221 cctgccgctg ctggcgctgc gcctgaaatc ggccgatctc gaccaattgg cggatgaatt
     8281 gcaggcgcag tacggcgaga tgccggactt cttcgaggac gacgccgtgg tgctcgacct
     8341 ctgcgccctg cccgaagaag ctgcggatgc cagcatcgac tttcagcgcc tgggcgagct
     8401 gctgcacgag cgccgcctgc gcctgctggc ggtgcgtggt ggcaacgcgg cgcacagcgc
     8461 cgccgccacc gacatcggcc tgctgctcgc gcccgatgcg cgcgtgcagg ccccgcccgc
     8521 acctgccgag ccggtgccca ccgccaaggc gccggtcgat gcagccgccg cgcacaattt
     8581 gcccgcgccc ggcgcgctgg tcatcgacaa gcccttgcgc tccggccagc aggtgtatgc
     8641 gcgcgggcgc gacctggtcg tgatggccat ggtcaacgcc ggtgccgagg tgattgccga
     8701 cggccacatc cacgtctatg ccccgctgcg cggccgcgcc attgccggcg cgcgcggctg
     8761 gcccgaggcg cgcattctgg cgcgcgagat gcggcccgag ttggtgtcca ttgccggcgt
     8821 ctaccgcgcc agcgacgagc cgctgccacc cgaagtctgg ggccgtccgg catccgtgca
     8881 gctggccagc tcggaggcgg gcgacaaact catcttcacg ccgatcgccg attgagggcg
     8941 atggcggctt tccaagcaac cgatcgaaca cgagaaccaa aggtgctctg acatggcaaa
     9001 aatcgtcgta gtgacctccg gcaagggcgg cgtgggcaag accaccacca gcgccgcgtt
     9061 cgcctccggc ctggcgctgg ccggccacaa gaccgccgtc atcgacttcg acgtcggcct
     9121 gcgcaacctc gacctca
//
LOCUS       DAOMVO010000073         9015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1973_length_9015_cov_16.869196, whole genome shotgun sequence.
ACCESSION   DAOMVO010000073 DAOMVO010000000
VERSION     DAOMVO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1973_length_9015_cov_16.869196"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..950)
                     /locus_tag="PKV30_05310"
     CDS             complement(81..950)
                     /locus_tag="PKV30_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRL29670.1"
                     /translation="MSDKAIITCSITGVLTDPAQHHVPVTPEQLAAEARRAYDAGASV
                     VHVHFRQQTPGKGHLPSWDPAVAKACVDAMRAACPGLIINQTTGVVGPDYQGPLDCLR
                     ATKPEMAACNAGSLNYLKTRSNGTWAWPPMLFDNQPAKVKDFLDVMAETGTLPEFECF
                     DVGIVRCVEMYVQTGMYAGLPEYNFVMGVESGMPADADLLPILLKLKIKDAPWQTTLI
                     GRSEIWPVHRRTAELGGHLRSGLEDTFYLPDGSKVTSNAPLIEQLATYAREAGRAVAS
                     PAEARQMLGLAVA"
     gene            complement(1011..2165)
                     /locus_tag="PKV30_05315"
     CDS             complement(1011..2165)
                     /locus_tag="PKV30_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL29671.1"
                     /translation="MTADELREARDALAATVERFARAEIAPHVTAWDAAGEFPRALYR
                     RAADLGLLGLGYPEAYGGTPAPHALRLPMWVALCRYGASGGVLASLLSHNIGLPPVLA
                     LGSEDVKREVIPPVLAGERIAALAITEPGGGSDVAALRTTAQREGGDYVVDGEKIFIT
                     SGMRADWITVAVRTGDGRGAKDISLLLVPGDSQGLSRTRLDKMGWLCSDTAHLRFDGV
                     RVPARYLLGDEGAGFKAIMGNFNAERFGIACAALGFSMACYDEALVWAQQRQTFGSPL
                     VGHQVVRHKLVDMQQRIRSTAAWLETVAARADAGDTGADWVGEVCVLKNHATQTLQFC
                     ADAGVQILGGMGYMRGTVCERIYREVKVLTIGGGTEEIMKDLAARQWHMI"
     gene            complement(2162..4138)
                     /locus_tag="PKV30_05320"
     CDS             complement(2162..4138)
                     /locus_tag="PKV30_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HRL29672.1"
                     /translation="MKRILIANRGEIARRIIATARRMGIETVAVYSEPDADALHVREA
                     TVAFALGGRTSAESYLRVDRLLEAARATGADAVHPGYGFLSEDATFARAVQDAGLTWI
                     GPPPAAIRALGSKSAAKALAAQHDVPCLPGYAGDDQSEARFATEAARIGSPLMVKAVA
                     GGGGRGMRLVEDAAQLPAALASARSEALAGFGNGDLLIERALLHPRHVEVQVFADAHG
                     HVIHLGERDCSVQRRHQKIIEEAPSPAVDADLRERMGACAVALARAAGYVGAGTVEFL
                     LEGDQFYLMEMNTRLQVEHPVTEALTGLDLVEWQIRVARGEPLPLTQSQVQFSGHAIE
                     VRLCAEDEQFHPHTGRVRHFAPPQAAAFSAAPLRFDHALTEGTEVSPYYDAMLGKLIV
                     HADTRAAAIDQLIAALRQLEVLGLPTNRAFLIECLQHPRFRSGQALISFLTTDVEGLR
                     AELHARERLLHERFGVLCVLGDASPLPCPYPAPRRLRHRGDERGVSIRSLGNGWWQVR
                     GDDGECSTLQRQGLPDGAAWVTTGSTARRVAAIAVPTADSPRRWHLQAEGVDAWLDDV
                     SFEPPAQAGQAQAATELRAPFNGKVLKIAAQPGQAMTAGEVALVIESMKLEHSLAPRA
                     DGVVAEVLVSVGQQVAPGQLLLRFAAPVKAEATA"
     gene            complement(4135..4959)
                     /locus_tag="PKV30_05325"
     CDS             complement(4135..4959)
                     /locus_tag="PKV30_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL29673.1"
                     /translation="MSDALLIERQPLGTGWAEFWTLNAPATRNALTDEMVWALRAACE
                     RAQCDTALRAVVLRGAGGHFCAGGSLGSFASAIGRPHGEGIDPLIAVNREFGHLLQAL
                     SALPQVLIAAVQGSAMGGGVGLVCCADHVLAATDAQFATPEVTLGIVPAQIALFLVRR
                     LGDATARDWLLSGVRWSAAEALSAGLANAVSDDDFEAALLQLLKRFALAAPGAVTQTK
                     QLLAAFAANQPLDAVLDDAALRFAQALRGPEAAQGLKAFAARQPAPWSQSLESITP"
     gene            complement(4977..6158)
                     /locus_tag="PKV30_05330"
     CDS             complement(4977..6158)
                     /locus_tag="PKV30_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL29674.1"
                     /translation="MQFTHDHEQIRDTLKRYIDEHINPHVDEWEEAGIFPAHQVFKGL
                     GDLGLLGLTKPEAYGGAGLDYSYGMVMAETLGHINCGGVPMAIGVQTDMCTPALARFG
                     SDELRKEFLMPAIAGDMVGCIGVSEPGAGSDVAGIKSVARKDGDDYVISGQKMWITNS
                     IQADWMCMLVNTSDGPAHKNKSLVMVPMRDGPNGQLTKGIEIAQKIRKIGMNSSDTGL
                     IYFDDVRVPQRNLIGQEGAGFVYQMQQFQEERLWAAASSLVTLTECVRKTIEWAQQRK
                     MFGATLIDQQWVQFKLAELQTEIEALRALTWRAGELYVGGQDVLQLASMAKLKAGRLT
                     REVADTCLQFWGGMGFTWENRVSRLYRDGRLGSIGGGADEVMLGIIAKTMGMAKKPTG
                     A"
     gene            complement(6360..8003)
                     /locus_tag="PKV30_05335"
     CDS             complement(6360..8003)
                     /locus_tag="PKV30_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL29675.1"
                     /translation="MTRFQSSFNAQGEAAQARRAAMLARIDTWRALEQRAADASAKAR
                     PQFDKRGQLLPRERVALLLDPGAPWLALCSLAGFMQDTKDPAKSVPGGGMIAGIGFIA
                     GMRCMVVASDSGIEAGAIQAMGLDKMLRVQEIALQNKLPFVHLVESAGANLMRYRVEG
                     FVHGGSLFRNLARLSSTGLPVITVQHGSGTAGGAYMPGLSDIVIMVRGRSRAFLAGPP
                     LLKAATGEVATEEELGGAEMHTSVSGLGEYLAEDDRQALGIARQVIGQIFESKVPLSP
                     VDQAQAAINSRAADSSFGVDDLLALMPAHHREPVDMREVMARLVDGSELLEFKALYGG
                     ATVCAQARIGGHAVGLISNNGPIDVPGANKATHFIQWMCQLGHPIIYLQNTTGYMVGK
                     DAEQGGMIKHGSKMIQAVTNATVPQITIQCGASFGAGNYGMCGRGFAPRFLFSWPSAK
                     TAVMGGEQAARTMQIVTEAALARKGIAVDAAESQKQFDQIVAMFEAQADAFHTSGLLL
                     DDGVIDPRDTRAVLAFCLDTIADGAARDVRTMQFGVARM"
     gene            complement(8000..8881)
                     /locus_tag="PKV30_05340"
     CDS             complement(8000..8881)
                     /locus_tag="PKV30_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL29676.1"
                     /translation="MSYRSVFAPGLFAGQVVVVTGGGSGIGRCTAHELAALGARVVLI
                     GRNADKLRAVQDEITADGGQASHAVCDIRQEDVVRQTVAGIVAQHGAIDGLVNNAGGQ
                     YITPAEKISAKGWQAVIDTNLTGGFLMARECFTQSMAARGGAVVNIVADIWGSMPGMA
                     HSGAARAGMVSFTETAALEWAGSGVRVNAVAPGYVASSGMDHYPPEAAAMLRQMPKTV
                     PLGRFATEAEVSAGIVFLLSPAASFISGTVLRIDGARPQVRMGWGQVAASADVQQRTA
                     VRPFDGFHRYQPPKVFQ"
     gene            complement(8878..>9015)
                     /locus_tag="PKV30_05345"
     CDS             complement(8878..>9015)
                     /locus_tag="PKV30_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL29677.1"
                     /translation="KARPEVARLFIFGALNWSVQWFSDRGALSLDDLTAQALLLFTEQ
                     A"
BASE COUNT         1481 a         3191 c         2830 g         1513 t
ORIGIN      
        1 gccctcgagg cggctttttt taatcgtaaa tgcgctccag cgctcgttgg ttaagcgcga
       61 gcagctatca aatcagcagt ctacgcaacc gccaagccca gcatctgacg cgcctcggcg
      121 ggcgatgcga cagcgcgccc ggcctcgcgc gcgtacgtgg ccagttgctc gatcaacggc
      181 gcattcgacg tgactttgct gccgtcgggc agatagaacg tgtcttccag cccgctgcgc
      241 aggtgcccgc ccaactcggc cgttcggcgg tgcaccggcc agatctcgct gcggccgatc
      301 aacgtggttt gccacggcgc gtccttgatc ttgagcttga gcaggatcgg cagcagatcg
      361 gcatcggccg gcatgccgga ttcaacgccc atgacgaagt tgtactccgg caaacccgcg
      421 tacatcccgg tttggacgta catctcgacg caccgcacga tgccgacatc gaagcactcg
      481 aactcgggca gcgtgcccgt ctcggccatc acgtccagaa agtccttcac cttggccggc
      541 tggttgtcga acagcatcgg cggccaggcc caggtgccgt tgctgcgcgt cttcaggtag
      601 ttcagcgagc ccgcattgca cgccgccatc tcgggtttgg tcgcgcgcag gcagtccagc
      661 gggccttgat agtccggccc gaccaccccg gtcgtctggt tgatgatgag gccggggcag
      721 gcggcgcgca tggcatccac gcaggctttg gcaaccgccg ggtcccacga cggcaggtgc
      781 cccttgcccg gcgtctgctg ccgaaagtgc acgtgcacca ccgacgcgcc cgcgtcgtag
      841 gcgcgccgcg cctcggccgc cagttgctcg ggcgtcacgg gcacgtggtg ctgggcgggg
      901 tcggtcagca cgccggtgat ggaacaggtg atgatggctt tgtcagacat agtgctcctc
      961 tattcgtagc tgcttgcgct tgattggcgc gcgccagggc cgaaaatctc ttaaatcatg
     1021 tgccactgac gggccgccag gtctttcatg atttcctcag tgccgccgcc gatggtcagc
     1081 accttgacct cgcggtagat gcgctcgcac acggtgccgc gcatgtagcc catgccgcct
     1141 agaatctgca cgccggcgtc ggcgcagaac tgcagggttt gggtggcgtg gttcttcagc
     1201 acgcagactt caccgaccca atcggccccg gtgtcgcccg cgtcggcgcg cgcggccaca
     1261 gtctccagcc aggccgctgt ggatcggatg cgctgctgca tgtcgacgag cttgtggcgc
     1321 accacctggt ggccgaccag ggggctgccg aaggtctggc gctgctgcgc ccagaccagc
     1381 gcctcgtcgt agcaggccat ggaaaagccc agcgcggcgc aggcgatgcc aaagcgctcg
     1441 gcgttgaaat tgcccatgat ggccttgaag cccgcgccct cgtcgcccag caggtagcgc
     1501 gcgggcacgc gcacgccatc gaagcgcagg tgcgcggtgt cgctgcacag ccatcccatc
     1561 ttgtcgaggc gcgtgcgact caagccctgg ctgtcgccgg gcacgagcag cagcgagatg
     1621 tccttcgcgc cccgcccgtc tcctgtgcgc acggccaccg tgatccagtc ggcgcgcatg
     1681 cctgaggtga tgaaaatctt ctcgccatcg accacgtagt cgccgccttc acgctgcgcc
     1741 gtcgtgcgca acgccgccac gtccgagccg ccacccggct cggtgatcgc cagcgcggca
     1801 atgcgctcgc cggccagcac gggcggaatc acttcgcgtt tcacgtcctc gctgcccagc
     1861 gccagcaccg gcggcaggcc gatgttgtgc gacagcaggc tggccagcac gccgccgctg
     1921 gcgccgtagc ggcacagcgc cacccacatc ggcaggcgca gcgcgtgcgg cgctggcgtg
     1981 ccgccatacg cctccggata gcccagaccc agcaggccca ggtcggcggc gcggcggtag
     2041 agcgcgcgcg gaaactcgcc ggccgcgtcc cacgcggtga cgtgcggcgc gatctcggcg
     2101 cgcgcaaagc gctcgacggt ggcggccagt gcgtcgcgcg cttcgcgcaa ctcgtccgca
     2161 gtcatgcggt tgcctccgct tttacgggcg ccgcaaagcg cagcagcaac tggccaggcg
     2221 cgacctgctg gcccacgctg accagcacct cggccaccac gccatcggca cgtggcgcca
     2281 ggctgtgctc cagcttcatc gattcgatga ccagcgccac ttcgcccgcc gtcatggcct
     2341 ggcccggctg cgcagcgatc ttcagcacct tgccgttgaa gggtgctctc agttccgtag
     2401 ctgcttgcgc ttgtcccgcc tgcgctggcg gctcaaaaga cacatcatcg agccacgcat
     2461 cgacgccttc ggcctgcagg tgccagcggc gcggtgagtc ggcggtgggc acggcaatgg
     2521 ccgccacgcg gcgagcggtg ctgccggtgg tcacccaggc ggcgccatcg ggcaggccct
     2581 gccgttgcag ggtgctgcat tcgccatcgt cgccgcgcac ttgccaccag ccgttgccga
     2641 gcgaccgaat cgacacgccg cgctcatcac cacgatgccg caggcggcgc ggcgcggggt
     2701 acgggcacgg cagtggcgag gcatcaccca gcacgcacaa gacgccaaag cgctcatgca
     2761 gcagccgctc gcgcgcgtgc aattcggcgc gcaagccctc gacatcggtg gtcaggaacg
     2821 agatcaaggc ctggcccgag cgaaagcgcg ggtgttgcag gcattcgatc aggaaggcgc
     2881 ggttggtcgg caggcccagc acttccagtt ggcgcaacgc ggcgatcaac tggtcgatgg
     2941 ctgcggcgcg cgtgtcggca tgcacgatca gcttgccgag catggcgtcg tagtagggcg
     3001 agacctccgt gccctcggtc agcgcgtggt cgaaacgcag cggcgcggcg ctgaaggcgg
     3061 cggcttgcgg cggcgcgaag tggcgcacgc ggccggtgtg cgggtgaaac tgctcgtctt
     3121 cggcgcacag gcgcacttcg atggcgtgac cgctgaactg aacttggctt tgcgtgagcg
     3181 gcagcggctc gccacgcgcc acgcggattt gccactcgac cagatcgagc ccggtcaacg
     3241 cctccgtcac cgggtgctca acttgcaggc gggtgttcat ctccatgaga tagaactgat
     3301 cgccttccaa caaaaactct acggtgccag cgcccacgta acccgccgca cgcgccagcg
     3361 ccaccgcaca cgcgcccatg cgctcgcgca ggtcggcatc gacggccggg ctgggcgctt
     3421 cctcgatgat cttctggtgg cgccgctgca ccgagcagtc gcgctcgccc aggtgaatca
     3481 cgtggccgtg cgcatctgca aacacctgca cctcgacgtg gcgcgggtgc agcagcgcgc
     3541 gctcgatcag cagatcgccg ttgccgaaac cggccagcgc ttctgatcgc gcactggcca
     3601 gcgcggccgg caattgcgcc gcgtcttcca ccaggcgcat gccgcgcccg ccgcccccgg
     3661 ccacggcctt gaccatcagc ggggagccga tgcgcgcggc ttctgtcgcg aagcgcgcct
     3721 cgctctggtc atcgcccgcg tagcccggca aacagggcac gtcgtgctgg gccgccagcg
     3781 ccttggccgc ggacttgctg cccagcgcgc gaatggcggc gggcggcggc ccgatccagg
     3841 tcaagccagc gtcctgcacg gcccgcgcga acgtcgcgtc ttcgctcaag aagccatagc
     3901 ccggatgcac cgcgtcggcg cccgtggccc gcgcggcttc cagcaagcga tcgacgcgca
     3961 gatagctttc ggccgaggtg cggccgccca gcgcaaaggc caccgtcgcc tcgcgcacgt
     4021 gcagcgcgtc ggcgtcgggc tctgaataca ccgccacggt ctcgatgccc atgcgccgtg
     4081 cggtggcgat gatgcggcgg gcaatttcgc cgcggttggc gatgaggatt cgcttcatgg
     4141 cgtgatgctt tcgagcgatt gcgaccacgg cgccggttgg cgcgcggcga aggctttcag
     4201 accctgcgcc gcttccggcc cgcgcagcgc ttgagcaaag cgcagggcag catcgtcaag
     4261 cacggcatcc aagggctggt tggctgcaaa ggcggccagc aattgcttgg tttgggtcac
     4321 ggccccaggc gcagccagcg caaagcgctt caatagttga agcaaggccg cttcaaaatc
     4381 atcatccgac actgcgttgg ccagaccggc cgacagcgcc tcagccgcgc tccagcgtac
     4441 accactcagc agccagtcgc gtgctgtggc atcgcccaac cgccgcacca aaaaaagcgc
     4501 gatttgcgcc ggcacgatgc ccagcgtgac ctcgggcgtg gcgaattgcg cgtcggtcgc
     4561 ggccagcaca tggtcagcgc agcacaccaa gcccacgccg ccgcccatgg cggaaccctg
     4621 cacggcggcg atcagcacct gcggcagcgc ggacagcgct tgcagcagat gcccgaactc
     4681 acggttgacg gcgatcagcg gatcaatgcc ctcgccatgc gggcgcccga tggcgctggc
     4741 gaaactgccg agacttccgc ccgcgcaaaa gtgcccgccg gcgccgcgca gcaccaccgc
     4801 gcgcaaggcc gtgtcgcact gtgctcgctc gcacgcggcg cgcagggccc acaccatctc
     4861 gtcggtcagc gcgttgcgcg tggccggtgc gttcaaagtc cagaactcgg cccagccagt
     4921 gccgagcggc tggcgttcga tcagcaaggc gtcgctcatc ttcagtgcct tttgcatcaa
     4981 gcgccggtgg gcttctttgc catccccatc gtcttggcga tgatgcccag catcacctca
     5041 tccgcgccac cgccgatgga gcccagacgc ccatcgcggt acaggcgcga aacgcggttc
     5101 tcccacgtga agcccatgcc gccccagaac tgcaggcagg tgtcggccac ctcgcgcgtc
     5161 aggcgccccg ccttcagctt ggccatgctg gccagttgca gcacgtcttg tccgcccacg
     5221 tacaactcgc cggcgcgcca ggtcagcgcg cgcagtgctt cgatttcagt ctgcaactcg
     5281 gccagcttga actgcaccca ttgctggtcg atcagcgtgg cgccgaacat cttgcgctgc
     5341 tgcgcccatt cgatagtctt gcgcacacat tcggtcaggg tgacgaggct gctggccgcc
     5401 gcccacaggc gctcttcctg gaactgctgc atctggtaga caaagcccgc gccttcttgc
     5461 ccgatgagat tgcgctgcgg cacacgcacg tcgtcgaaat agatcagacc cgtgtcggac
     5521 gaattcatgc cgatcttgcg gatcttctgc gcaatctcga tgcccttcgt cagctggcca
     5581 ttggggccgt cgcgcatcgg caccatcacc aggctcttgt tcttgtgcgc gggcccgtcg
     5641 ctggtgttga ccagcatgca catccagtcg gcctgaatgc tgttggtgat ccacatcttc
     5701 tggccactga tgacgtaatc atccccatcc ttgcgggcca ccgacttgat gcccgccacg
     5761 tcgctgcccg cgcccggttc actcacccca atgcagccca ccatgtcgcc cgcgatggcg
     5821 ggcatgagga actcttttcg cagctcatcg ctgccaaagc gcgccagcgc gggcgtgcac
     5881 atgtccgtct gcacgccaat cgccatcggc acgccgccgc agttgatgtg gcccagcgtc
     5941 tcggccatga ccatgccgta ggaatagtcg aggcccgcgc cgccataggc ctcgggcttg
     6001 gtcagaccca gcaaacccag atcgcccaaa cccttgaaca cctggtgggc ggggaagatg
     6061 ccggcctctt cccattcatc gacgtgcggg ttgatgtgct cgtcgatgta gcgtttgagc
     6121 gtgtcgcgga tttgctcgtg gtcgtgggtg aattgcatct tgtctcctca gaactctcta
     6181 aattcaacag ccggacgcga aggacgcaaa ggattcgcga aggacgcgaa agaagtcaaa
     6241 aattcttggc gtgaatttgc aagtgcgacg taccttctgg caaaagtact ctttgaattt
     6301 ttcgcgtcct tcgcgtcgtc tttgcgtcgt tcgcgtacgg atgttcggct gctgggtatt
     6361 cacatccgcg ccacgccaaa ctgcatagtc cgcacatcgc gcgccgcacc gtcggcaatc
     6421 gtgtccaggc aaaacgccag cacagcgcgc gtgtcgcgcg ggtcgatcac gccgtcgtcc
     6481 agcagcagac cacttgtatg gaacgcatcg gcctgcgcct cgaacatagc gacgatctgg
     6541 tcgaactgct tttgcgattc agctgcgtcg accgcgatgc ctttgcgcgc cagcgcggct
     6601 tcggtgacga tctgcatggt gcgggcggcc tgctcgccgc ccatcacggc cgtcttcgcg
     6661 ctcggccaac tgaacagaaa gcgcggcgca aaaccgcgcc cgcacatgcc gtagttgccg
     6721 gcgccaaagc tggcgccgca ctggatggtg atctgcggca cggtggcgtt ggtcacagcc
     6781 tgaatcatct tgctgccgtg cttgatcatg ccgccctgct cggcgtcctt gccgaccatg
     6841 tagcctgtcg tgttctgcag atagatgatc gggtggccca gctggcacat ccactggatg
     6901 aagtgcgtgg ccttgttggc gccgggcacg tcgatgggcc cgttgttgct gatcaggccc
     6961 accgcgtggc cgccgatgcg cgcctgcgca cacacggtgg cgccgccata cagcgccttg
     7021 aattcgagca gctcggaccc atcgacaagc cgcgccatca cctcgcgcat gtccaccggc
     7081 tcgcggtggt gcgcgggcat cagggcgagc aggtcatcga caccaaagga tgaatcagca
     7141 gctcttgaat ttatagctgc ttgcgcttga tcaacgggcg acagaggcac ttttgactca
     7201 aaaatctgcc cgatcacctg tcgtgcaatg cccagcgcct gccggtcatc ttccgccagg
     7261 tattcgccca gcccggacac gctggtgtgc atctcggcgc cgcccagttc ttcttccgtc
     7321 gccacttcac cggtcgccgc cttcaatagt ggcggcccag ccagaaaggc gcgcgaacgg
     7381 ccgcgcacca tgatcacgat gtccgacagc cccggcatgt acgccccgcc cgccgtgccc
     7441 gatccgtgct gcaccgtgat caccggcaag cccgtgcttg agagccgcgc caggttgcgg
     7501 aacaggctgc caccgtgcac aaagccctcg acgcggtagc gcatcaggtt ggcacccgcg
     7561 ctttccacca ggtgcacaaa gggcagcttg ttctgcagcg cgatttcttg cacgcgcagc
     7621 atcttgtcca ggcccatggc ctgaatggcg ccggcttcaa tgcccgaatc gctggccacc
     7681 accatgcagc gcataccggc aatgaagccg atgccggcga tcatgccgcc gcccggcacc
     7741 gacttggccg ggtctttcgt gtcctgcatg aagcccgcca gcgaacacag cgccagccaa
     7801 ggcgcgcccg ggtccagcag cagcgccacc cgctcgcgcg gcagcaactg gccgcgcttg
     7861 tcgaactgcg gcctggcctt ggcggatgcg tcggcggcgc gctgctccag cgcgcgccag
     7921 gtgtcgatgc gtgccagcat cgccgcgcgc cgcgcctgcg ccgcttcgcc ttgcgcgttg
     7981 aaagaggact gaaagcgcgt cattgaaata ccttgggcgg ctggtaacgg tggaaaccat
     8041 cgaacgggcg caccgcagtg cgttgctgca catcggccga agccgccacc tggccccagc
     8101 ccatgcgcac ctgcggccgc gcgccgtcga tgcgcagcac cgtgccactg atgaaactgg
     8161 ccgccgggct gagcaaaaac acaatgccgg ccgaaacttc cgcctcggtc gcaaagcggc
     8221 ccaacggcac cgtcttgggc atctggcgca acatggccgc cgcctcgggc gggtaatggt
     8281 ccatcccgct ggatgccaca tagcccggcg ccaccgcgtt cacgcgcaca ccgctgccgg
     8341 cccactccaa ggcggcggtt tcagtgaagc tcaccatgcc cgcgcgcgcc gcgccgctgt
     8401 gcgccatgcc gggcatcgag ccccagatgt cggccacgat gttcacaacc gcgccgccgc
     8461 gcgccgccat gctttgcgtg aagcactcgc gcgccatcaa aaagccgccg gtcagattgg
     8521 tgtcgatcac cgcctgccag cccttggcgc tgatcttctc ggccggggtg atgtactggc
     8581 cgcccgcgtt gttcaccagc ccatcgatcg cgccgtgctg cgccacgatg cccgccacgg
     8641 tctgtcgcac tacatcctcc tgccgaatgt cgcacaccgc gtggctggcc tggccgccat
     8701 cggcggtgat ttcatcctgc accgcgcgca gcttgtcggc gttgcggcca atcagcacca
     8761 cgcgcgcgcc cagcgccgcc agctcgtgcg ccgtgcagcg cccaatgccc gagccgccgc
     8821 ccgtcaccac caccacctga ccggcgaaca ggccgggcgc gaagaccgat cggtaactca
     8881 tgcttgctcc gtgaaaagca gcaaggcctg tgccgtgagg tcatccagcg acagcgcgcc
     8941 ccggtctgaa aaccattgca ccgaccagtt gagcgcgcca aagatgaaca gccgcgccac
     9001 ctctggccgc gcctt
//
LOCUS       DAOMVO010000074         9007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_1974_length_9007_cov_14.521671, whole genome shotgun sequence.
ACCESSION   DAOMVO010000074 DAOMVO010000000
VERSION     DAOMVO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_9007_cov_14.521671"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKV30_05350"
     CDS             <1..206
                     /locus_tag="PKV30_05350"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461113.1"
                     /note="catalyzes the formation of 2-acetolactate from
                     pyruvate, leucine sensitive; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HRL29678.1"
                     /translation="VKLAEAFGHVGMLIERPQDVEPALREARKLKDRTVFMDFRTDPT
                     ENVFPMVQAGKGITEMLLGSEDL"
     gene            335..826
                     /gene="ilvN"
                     /locus_tag="PKV30_05355"
     CDS             335..826
                     /gene="ilvN"
                     /locus_tag="PKV30_05355"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRL29679.1"
                     /translation="MKHIIAVLIENEAGALSRVVGLFSARGYNIESLIVAPTEDESLS
                     RMTIQTRGSDEVIEQITKHLNRLIEVVKVVDLTEGSYIEREHMMVKVRAVGKEREEIK
                     RMADIFRGRIIDVTDKSYTIELTGDQGKHDAFLNAIDKTAILETVRTGASGIGRGERL
                     LKV"
     gene            900..1916
                     /gene="ilvC"
                     /locus_tag="PKV30_05360"
     CDS             900..1916
                     /gene="ilvC"
                     /locus_tag="PKV30_05360"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRL29680.1"
                     /translation="MKVFYDKDCDLSLIKGKTVAIIGYGSQGHAHAQNLNDSGVKVVV
                     GLRKGGASWPKVEKAGLKVAEVADAVKQADVVMILLPDEQIAKVYREDIEPNIRQGAS
                     LAFAHGFNVHYGQVVPRADLDVWMVAPKAPGHTVRNTYTQGGGVPHLVAVHQDKTGKA
                     RDLALSYAMANGGGKAGIIETNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPRIVTDETKKAMKQ
                     ALRDIQTGEYAKSFIAENLAGAPTLQSRRRLNAESDIEVVGEKLRAMMPWIKANKLVD
                     KSKN"
     gene            2055..2915
                     /gene="pssA"
                     /locus_tag="PKV30_05365"
     CDS             2055..2915
                     /gene="pssA"
                     /locus_tag="PKV30_05365"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRL29681.1"
                     /translation="MVTDPDEVVITRKRRKGIYVLPNLFTLAALFGGFYAIVMAMNGQ
                     FDLAATGIFAAMVLDSLDGRVARMTGTQSAFGEQMDSLSDMVSFGAAPALVAYEWALR
                     PLGRWGWIAAFVYCACAALRLARFNVNTLVVDKRYFQGLPSPAAAALVAGFIWLTSAM
                     LVAHREVPIFRALISMFGGVRVERVDFAWIMFGVTLFAGLTMVTNIPYYSFKDFGGRR
                     SVPFAVLVLLALLIALISLEPATLLFVLFVGYALSGYAIYVWRRAKGHQVSIVSTSTE
                     EPDERGLHHD"
     gene            3149..4687
                     /locus_tag="PKV30_05370"
     CDS             3149..4687
                     /locus_tag="PKV30_05370"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRL29682.1"
                     /translation="MTDQLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLKVDVIE
                     AGFAASSNGDFECVKAIAEAIKDSTVCSLSRANDRDIARAAEALKGAARGRIHTFIAT
                     SPLHMEKKLRMTPDEVFEQSKLAVRFARNLMGDVEFSAEDGYRSDIDFLARVCEAVIK
                     EGATTINIPDTVGYAIPELYGNFIKTLREKVPNSDQAIWSVHCHNDLGMAVANSLAGV
                     KLGGARQIECTINGLGERAGNCSLEEIVMAVKTRKDYFGLDVGIDTSQIVPASKMVSH
                     TTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSG
                     RNAFKQRLQELGVMLESEGEIMAAFAKFKELADRKSEIFDEDILALVGDESVTPEKEH
                     FGFLSLAQRSETGEMPHAEIVFTVGGQEVRGQSDGNGPVDASLKAIESHVKSGAEMIL
                     YSVNAISGSTESQGEVTVRLQNSGRVVNGVGADPDIVVASAKAYLSALNKLHDKSDRV
                     AAQG"
     gene            4843..6150
                     /locus_tag="PKV30_05375"
     CDS             4843..6150
                     /locus_tag="PKV30_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRL29683.1"
                     /translation="MWRGVGLLSASLILSLTTLAPAAAAPAKKAKAADARPAAKAKPA
                     APSKSTAAKPASKQVAAKATSGKAAKNAPQVAAKGKAAKADVQEAKASKRDRGGKSAK
                     RAFAEERRGGKTNVARGSDRVQRTLSARGKANERAERMAARETRLAIRTVAHVPQEAR
                     PARLSFGQLAGLRGVEDPLELKSSVALVLDQDTHEVLLSKNDHAVLPIASLTKLMTGL
                     IVSDARLPLDEMISITEDDIDYEKGSRSRLPVGTTLTRGELMHLALMSSENRAAHALG
                     RTFPGGMQRFVGLMNARAMMLGMKDTRYVEPTGLSSENRSSAYDLATLVNTAAQDPLL
                     RQLSTSQGYEVAVGNRVLQYNNTNRLVHNPDWQIGLQKTGYISEAGQCLVMQARVAGR
                     KLIMVFLDSSGKYSRLADAERVRRWVEFERAPTNTAASNPYAS"
     gene            complement(6262..7044)
                     /locus_tag="PKV30_05380"
     CDS             complement(6262..7044)
                     /locus_tag="PKV30_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRL29684.1"
                     /translation="MRRGNPDVHQTPTIQVIERMFSLIDVLASREDSMSLKDISEQTG
                     LHPSTAHRILNDLTIGRFVDRPEAGSYRLGMRLLELGNLVKARLSVRDAALVPMRDLH
                     RLIQQPVNLSVRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSTGKLFLAAEEAQ
                     RVRAYATRTGLAGHTRNSITQLPALERELVTARQSGVARDNEELELGVRCMAAGIYDD
                     QGKLVAGLSISSPADRLDDSWLPKLKQTAQQISTALGHDDRG"
     gene            complement(7050..7832)
                     /locus_tag="PKV30_05385"
     CDS             complement(7050..7832)
                     /locus_tag="PKV30_05385"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRL29685.1"
                     /translation="MTHLKELPLQALQFYATAPYPCSYLQGRQARSQVATPSHLINND
                     AYSGLVQGGFRRSGMFTYRPYCDGCAACVPVRVLAQHFQPDRSQRRARARHANLQVRV
                     LKLGYVPEHYQLYLRYQSGRHSGGGMDHDSVDQYTQFLLQSHVNSRLVEFREPGADDA
                     PGRLRMVSIVDVLGDGLSAVYTFYDPEPRASYGTYNILWQIEQARTLGLPYVYLGYWI
                     EGSGKMAYKARFRPLQRLLSGSWQGALPEALAPDAPTAGLAG"
     gene            complement(7829..8590)
                     /gene="aat"
                     /locus_tag="PKV30_05390"
     CDS             complement(7829..8590)
                     /gene="aat"
                     /locus_tag="PKV30_05390"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRL29686.1"
                     /translation="MNQRPKLALLQPGDAFPPLSQAWPAHSAAPGLIAAGGVLDVPTL
                     VAAYSATIFPWFSKGEPTLWWSPDPRMVLQVDRFRLHRSLRRVLQAFRLNANCEIRID
                     TAFDTVVAACAAATRPAQTGTWIGPEMQAAYRALHRAGHAHSVETWVGGRLVAGLYCV
                     AIGRAVFGESMFTHVTDGSKIALSALVALCRVQGRPLIDCQQNTQHLAFMGAAEMPRA
                     EFAAAVQQLVREPDAPWRFEPVYWNALMPHSPAER"
     gene            complement(8601..>9007)
                     /locus_tag="PKV30_05395"
     CDS             complement(8601..>9007)
                     /locus_tag="PKV30_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL29687.1"
                     /translation="GTVPYLGDQVLLCKRAIEPRYGKWTLPAGFMELGETMAQGAARE
                     TAEEAGAEVEMGAFFSAMSIPRVGQVHVFYLARLLHERFDPGHESLEARLFTEGDLPW
                     DELAFTTVRETLKCFFADRRLGQFGLHDVDIT"
BASE COUNT         1685 a         2830 c         3000 g         1492 t
ORIGIN      
        1 tcgtgaagct ggccgaggcc tttggccacg tcggcatgct gatcgagcgc ccgcaagatg
       61 tagagccggc gctgcgtgaa gcgcgcaagc tgaaagatcg caccgtgttc atggacttcc
      121 gcaccgaccc gacggagaac gtgttcccca tggtgcaggc cggcaagggc atcaccgaga
      181 tgctgctggg ctccgaggat ttataagaaa taagccgcca gcgcttgcct gacaagcgcg
      241 agcagctatt taatttgtag tagacgattc tattgctcgc caagcccgcc gcttaccggc
      301 tgcgggggag ggcgacgaaa agagggctct gaaaatgaaa cacatcatcg ctgttctgat
      361 tgaaaacgag gcgggcgcgc tgtcccgcgt ggtgggcttg ttctcggccc gtggctacaa
      421 catcgaatcg ctcatcgtcg cgccgaccga ggacgaaagc ctctcgcgca tgaccatcca
      481 gacgcgcggc tccgacgaag tcatcgagca gatcaccaag cacctgaacc gcctgatcga
      541 ggtggtcaag gtggtcgacc tgaccgaagg cagctacatc gagcgcgagc acatgatggt
      601 gaaggtgcgg gcggtcggca aggagcgcga agagatcaag cgcatggccg acatcttccg
      661 cggccgcatc atcgacgtca ccgacaagag ctacaccatc gagctgaccg gcgaccaggg
      721 caagcacgac gccttcctca acgccatcga caagacggcc attctggaaa ccgtgcgcac
      781 cggcgccagc ggcatcgggc gcggcgagcg cttgttgaag gtttgagcct gcagcgcttg
      841 ccagacaagc gcgagtagct tttatttgta tagcaacgag atcatcaaag gagaaacccg
      901 tgaaagtgtt ctacgacaaa gactgcgacc tgagcctgat caagggcaag accgtcgcca
      961 tcatcggcta cggctcgcag ggccatgcgc acgcgcagaa cctgaacgat tcgggcgtca
     1021 aggtcgtggt cggcctgcgc aagggcggcg cgagctggcc caaggtcgag aaggccggcc
     1081 tgaaagtcgc cgaagtggcg gacgccgtga agcaggccga cgtggtcatg atcctgctgc
     1141 ccgacgagca gatcgccaag gtctaccgcg aagacatcga gccgaacatc aggcagggcg
     1201 cgtcgctggc gtttgcgcac ggcttcaacg tgcactacgg ccaggtcgtg ccgcgcgccg
     1261 atctggacgt gtggatggtc gcgcccaagg cgcccggcca caccgtgcgc aacacctaca
     1321 cgcaaggtgg cggcgtgccg cacctggtgg cggtgcatca ggacaagacc ggcaaggcgc
     1381 gtgacttggc cctcagctac gccatggcca acggcggcgg caaggccggg atcatcgaaa
     1441 ccaacttccg cgaagagacc gaaaccgacc tgttcggcga gcaggccgtg ctgtgcggcg
     1501 gtacggtgga gctgatcaag gctggcttcg aaacgctggt cgaggccggc tacgcgcccg
     1561 aaatggccta cttcgagtgc ctgcacgagc tgaagctgat cgtcgatttg atctacgaag
     1621 gcggcatcgc caacatgaac tactcgatca gcaacaacgc cgaatacggc gaatacgtga
     1681 ccggcccgcg catcgtcacc gacgaaacca agaaggccat gaagcaggcg ctgcgcgaca
     1741 tccagaccgg cgaatacgcc aagagcttca tcgccgaaaa cctggccggc gcgcccacgc
     1801 tgcaaagccg ccgccgcctg aacgccgaga gcgacatcga agtggtgggc gaaaaactgc
     1861 gcgccatgat gccctggatc aaggccaaca agctggtcga caagagcaag aactgacgca
     1921 ccgcggcgcg gtgtttgcac acgggccacg cggtatgcgt ggcctttttg tttagatatg
     1981 cttttgatag ctgcttgcgc ttgtcctgcc tgcgccagcg gcatttttta cccttgaaag
     2041 taaaatcggc acccatggtt accgatcccg acgaagtcgt catcacacgc aagcggcgca
     2101 agggcatcta cgtgctgccc aacctgttca cgctggcggc gttgtttggt ggtttctacg
     2161 ccatcgtgat ggcgatgaac ggccagttcg acctggccgc caccggcatc tttgccgcca
     2221 tggtgctcga cagtctggac ggccgcgtgg cgcgcatgac gggcacgcaa agcgcgttcg
     2281 gcgagcagat ggattcgctg tccgacatgg tcagctttgg cgccgcgcca gcgctggtcg
     2341 cctacgaatg ggcgctgcgc ccgctcggcc gctggggctg gatcgccgcc tttgtctact
     2401 gcgcgtgcgc ggcgctgcgg ctggcgcgct tcaacgtcaa cacattggtg gtcgacaagc
     2461 gctatttcca gggcctgccg tcgccggcgg cggcggcgct ggtggccggc ttcatctggc
     2521 tgaccagcgc catgctggtg gcgcaccgcg aggtgccgat cttccgcgcg ctgatctcga
     2581 tgtttggcgg cgtgcgggtc gagcgggtgg acttcgcctg gatcatgttc ggcgtcaccc
     2641 tgtttgccgg attgaccatg gtcaccaaca tcccctacta cagcttcaag gatttcggcg
     2701 gccggcgcag cgtgcccttt gctgtgctgg tgctgctggc gctgctgatc gccctcatca
     2761 gcctggagcc ggcgacgctg ctgttcgtgc tgtttgtggg ctacgcgctg tcgggctacg
     2821 ccatttacgt atggcggcgc gccaaagggc atcaggtgag catcgtcagc acctcgaccg
     2881 aagagcccga cgagcgcggg ctgcaccacg attgaggcgg gcacgcgtgg cgaaatttcc
     2941 gctgcgcgtt gccgatttcc tgtgctacat ttgcatcccg tatgaacaag tcgctggctc
     3001 tactactggc aacgccgaac gggcggagat agtagcgcgc gcaacttttt ccattaaagg
     3061 cccgtttgca cccgcagcgg gcctttttgt tttgttcgaa catttttcgc tcgcgggacc
     3121 ttaagcgttc aatcttggag aagctttcat gaccgaccaa ctcatcatct tcgacaccac
     3181 cttgcgcgat ggcgaacagt cacccggcgc cagcatgacg cgcgacgaga agctgcgcat
     3241 cgctcgccag ctggagcgcc tgaaggtcga cgtgatcgag gccggctttg ccgcatcttc
     3301 caacggcgac ttcgagtgcg tgaaggcgat tgccgaggcg atcaaggatt ccaccgtgtg
     3361 ctcactcagc cgcgccaacg accgcgacat cgcgcgcgcc gcggaggcgc tgaagggcgc
     3421 ggcgcgcggg cgcatccaca ctttcattgc caccagcccg ctgcacatgg agaaaaagct
     3481 gcgcatgacg cccgacgagg tgttcgagca gtctaagctg gcggtgcgct ttgcgcgcaa
     3541 cctgatgggc gacgttgagt tcagcgccga agacggctac cgcagcgaca tcgactttct
     3601 ggcccgcgtg tgcgaagcgg tgatcaagga aggcgcgacc accatcaaca ttcccgacac
     3661 ggtgggctac gccatccccg agctgtacgg caacttcatc aagacattgc gcgagaaggt
     3721 gcccaacagc gaccaggcga tctggtcggt gcactgccac aacgatctgg gcatggccgt
     3781 ggccaacagc ctggcgggtg tgaagctcgg cggcgcgcgg cagatcgaat gcaccatcaa
     3841 cggcctgggc gagcgggcgg gcaactgctc gctggaagag atcgtgatgg cggtgaagac
     3901 gcgcaaggat tactttggcc tggatgtcgg catcgacacg tcgcaaatcg tgccggccag
     3961 caagatggtg agccacacca ccggcttcgt ggtgcagccc aacaaggcgg tggtgggcgc
     4021 caacgccttt gcgcacgcca gcggcatcca ccaggatggc gtgctgaagg cgcgcgacac
     4081 ctacgaaatc atgcgcgccg aggacgtggg ctggagcgcc aacaagatcg tgctgggcaa
     4141 gctctcgggc cgtaacgctt tcaagcagcg cttgcaggag ttgggcgtga tgcttgaatc
     4201 cgaaggcgaa atcatggccg cgtttgccaa gttcaaggaa ctggccgacc gcaagagcga
     4261 gatcttcgac gaagacattc tggcgctggt gggcgatgaa agcgtgacgc ccgagaagga
     4321 gcatttcggt tttctgtcgc tcgcgcagcg cagcgaaacc ggtgagatgc cgcacgccga
     4381 aatcgtgttc accgtaggcg ggcaggaagt gcgcggccag tccgacggca acgggccggt
     4441 cgatgcgtcg ctgaaagcca tcgaatcgca cgtgaaaagc ggcgccgaga tgattctgta
     4501 ctcggtcaac gccatcagcg gctcgaccga gagccagggc gaggtcaccg tgcggctgca
     4561 aaactcgggc cgggtggtga acggcgtggg ggccgacccc gacatcgtgg tcgcctcggc
     4621 caaggcttat ctcagcgccc tcaacaagtt acacgacaaa tctgatcggg tggcggcgca
     4681 aggctagggt taataccgat aactgttaca tcggttgagg aaagagacgc aagtcaatga
     4741 tattgcgtct ctttttttgt ttgagtacac tttcgcatcg ttggcggctc ggtttgttgg
     4801 gggattgcga tgcgtggtgc gttcaaacgg cgtctggcgc ctgtgtggcg gggggtcggc
     4861 ctgctgtcgg ccagcctgat cctgagtctc accacgctgg cgccggccgc cgcggcaccg
     4921 gccaagaagg ccaaggccgc cgacgcgcgg cccgccgcca aggccaaacc cgccgcgccc
     4981 agcaagtcca cggccgccaa gcccgccagc aaacaggttg ccgccaaggc gaccagcggc
     5041 aaggcggcga agaacgcgcc ccaagtggcg gccaagggca aggccgccaa ggccgatgtg
     5101 caagaagcca aggccagcaa gcgcgaccgc ggtggcaagt cggccaagcg cgcctttgcc
     5161 gaggagcggc gcggcggaaa aacgaacgtt gcgcgtggca gcgaccgcgt gcagcgcacg
     5221 ctgtcggcgc gcggcaaggc gaatgagcgc gccgagcgca tggccgcgcg cgagacgcgg
     5281 ctggcgattc gcaccgtcgc ccatgtgccg caggaggcgc gaccggcacg cctgtcgttc
     5341 ggccagctgg cgggcctgcg cggggtggaa gacccgctgg agttgaagtc gagcgtggcg
     5401 ctggtgctgg accaggacac gcacgaagtg ctgctcagca agaacgacca cgcggtgctg
     5461 ccaattgcct cgctcaccaa gctgatgacc ggcctgatcg tgtccgatgc gcgcctgccg
     5521 ctggacgaga tgatcagcat cactgaggac gacatcgatt acgagaaggg cagtcgctca
     5581 cgcctgccgg tgggcaccac gctgacgcgc ggcgaattga tgcacctggc gctgatgtcg
     5641 agcgagaacc gcgctgcgca tgcgctgggc cgcacctttc cgggcggcat gcagcgcttt
     5701 gtcggcctga tgaacgcccg cgccatgatg ctgggcatga aggacacgcg ctacgtcgag
     5761 ccaaccggcc tgtcgagcga gaaccgctcc agcgcctacg acctggcgac gctggtgaac
     5821 acggccgcgc aagacccgct gctgcgccag ctgtcgacct cgcaaggcta tgaagtggcg
     5881 gtgggcaacc gcgtgctgca gtacaacaac accaaccggc tggtgcacaa cccggactgg
     5941 cagatcggcc tgcagaagac cggctacatc tctgaagccg gtcagtgcct ggtgatgcag
     6001 gcgcgcgtgg cggggcgcaa gctgatcatg gtgttccttg attcgagcgg caagtacagc
     6061 cgcctggccg acgccgagcg cgtgcgccgc tgggttgagt tcgagcgcgc gccgaccaac
     6121 acggcggcca gcaaccccta cgcgagctga gcgcagattt tcattcgtcc tgcttttaat
     6181 ttgatagctg ctcgcgcttg attggcaagc gctggagctc taaatcattc aagaaattga
     6241 tggcgccgcc aagcgccccg ctcagccgcg gtcgtcgtgg cccagcgccg tcgaaatctg
     6301 ctgcgccgtc tgcttgagct tgggcagcca gctgtcgtcc agccggtcgg cgggcgatga
     6361 gatcgacagg ccggccacca gcttgccctg gtcgtcgtag atgccggcag ccatgcagcg
     6421 cacgcccagt tccagctcct cgttgtcgcg cgccacgccg gactggcggg cagtgaccag
     6481 ttcgcgctcc agcgccggca gttgcgtgat gctgttgcgc gtgtggccgg ccaggcccgt
     6541 gcgcgtggcg taggcgcgca cgcgctgagc ttcttcggcg gccaggaaga gcttgccggt
     6601 ggacgtcaaa tgcagcggcg cgcggccgcc aatggcgcgc accacctgca tgccggagcg
     6661 ttcgctgtag gcgcgctcga tgtaaacaat ctcgtcaccc tggcgcacgc tcaggttgac
     6721 cggctgctgg atcaggcggt gcagatcgcg catcggcacc agcgcggcgt cgcgcaccga
     6781 caggcgcgcc ttgaccaggt tgcccagctc caaaaggcgc atgcccagcc ggtagctgcc
     6841 ggcctcaggg cgatcaacga agcgcccgat ggtcagatcg ttcaggatgc ggtgcgcggt
     6901 ggacgggtgc aggccggtct gctcgctgat gtccttgagc gacatcgaat cctcgcgcga
     6961 ggccagcacg tcgatgagcg aaaacatgcg ctcgatgacc tggatggtcg gcgtctggtg
     7021 aacgtcgggg ttgccgcggc gcatggcaat caaccggcca atccagcggt tggcgcatcc
     7081 ggcgccagcg cttcgggcag cgcgccttgc cagctgccgc tcagcagccg ctgcagcgga
     7141 cgaaaacgcg ccttgtaagc catcttgccg ctgccctcga tccagtaacc caggtacaca
     7201 tagggcaggc ccagcgtgcg cgcctgctcg atctgccaca ggatgttgta ggtgccgtag
     7261 cttgcgcgcg gctcggggtc gtagaaggtg tagacggccg acaggccatc gcccagcacg
     7321 tcgacgatgg agaccatgcg caggcggccc ggggcgtcgt ccgcgcctgg ttcgcggaac
     7381 tccaccagcc gcgagttgac gtggctttgc agcagaaatt gggtgtattg atcgacgctg
     7441 tcgtggtcca tgccgccgcc ggagtggcgg cccgactggt agcgcaggta gagctgatag
     7501 tgctcgggca cgtagccgag cttgagcacc cgcacctgca gattggcgtg gcgcgcccgc
     7561 gcgcggcgct ggctgcggtc gggctgaaag tgctgggcca gcacgcgcac cggcacgcag
     7621 gccgcgcagc cgtcgcaata cggccggtag gtgaacatgc cgctgcgccg aaaaccgcct
     7681 tgcaccaggc cggaataggc gtcgttgttg atcaggtgac tgggcgtggc gacttgcgaa
     7741 cgtgcttgcc tgccctgcaa ataactgcaa gggtaggggg ccgtggcgta gaactgcagg
     7801 gcctggagcg gaagttcctt gaggtgcgtc accgttcagc ggggctgtgg ggcatcagtg
     7861 cattccagta tacgggctcg aatcgccagg gcgcatctgg ctcgcgcacc agttgctgca
     7921 cggcggcggc gaattcggca cgcggcattt cggcggcgcc catgaaggcc aggtgctgcg
     7981 tgttctgctg gcagtcgatc aacggccggc cctgcacccg acacagcgcc accagcgccg
     8041 acagcgctat ttttgacccg tcggtcacgt gggtgaacat ggattcgcca aacaccgcgc
     8101 gcccgatcgc cacgcaatac agcccggcca ccaggcgccc gccgacccag gtttcaacgc
     8161 tgtgcgcgtg cccggcgcgg tgcagcgccc ggtaggcggc ctgcatctcg ggcccgatcc
     8221 aggtgccggt ttgcgccggc ctggtggcgg ctgcgcacgc ggcgacgacg gtatcgaaag
     8281 cggtgtcgat gcggatttcg cagttggcgt tgagccgaaa cgcctgcagc acccggcgca
     8341 gcgagcggtg caggcgaaac cgatcgacct gcagcaccat gcgcgggtct gggctccacc
     8401 acagcgtcgg ctcgcctttg ctgaaccacg gaaaaatcgt tgccgaatag gccgccacca
     8461 gcgtcggcac gtcaagcacg ccgccggccg cgatcaagcc gggcgccgcc gaatgcgcgg
     8521 gccaggcctg tgacagcggc ggaaaggcgt cgcccggctg cagcagcgcg agcttcggcc
     8581 gctggttcat gggctgggct tcaggtgatg tcgacgtcat gcaggccgaa ctggcccagc
     8641 cgccggtccg caaagaagca cttgagcgtt tcgcgcacgg tggtgaaggc cagctcgtcc
     8701 cacggcaaat cgccttcggt gaacaggcgc gcttccagcg actcgtggcc agggtcgaag
     8761 cgctcgtgca gcagccgcgc gagataaaac acgtggactt gcccgacacg cggaatgctc
     8821 atggcgctga agaaagcgcc catctcgacc tcggcgccgg cttcttcagc cgtttcgcgc
     8881 gccgcgccct gcgccatggt ttcgcccagc tccatgaagc cggccggcag cgtccatttg
     8941 ccgtagcgcg gttcgatggc gcgcttgcac agcagcacct ggtcacccag gtaaggcacg
     9001 gtgccca
//
LOCUS       DAOMVO010000075         8932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2001_length_8932_cov_13.356427, whole genome shotgun sequence.
ACCESSION   DAOMVO010000075 DAOMVO010000000
VERSION     DAOMVO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_8932_cov_13.356427"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..715)
                     /locus_tag="PKV30_05400"
     CDS             complement(2..715)
                     /locus_tag="PKV30_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRL29688.1"
                     /translation="MDDPILTMEEREAINTGRWFSSLSPSLRHDILRCAYVKRYPDGD
                     LIAARGEPAEQWIACAKGAVRVSSTTLTGKQITLTYVEPGIWFGDVAIFDGDRRTHDA
                     YAHGATTILCVARADFHKILAQHPELYDALLKLQARRIRLLFGQVEDLNTLPLRARLA
                     KQLGHLVRSYGVPSLSSNDEWRITLQLAQEELAQLLGASRQRVNQELKSMEREGVIRV
                     EPTGLVVCDRDALLRIGEE"
     gene            complement(816..1595)
                     /locus_tag="PKV30_05405"
     CDS             complement(816..1595)
                     /locus_tag="PKV30_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRL29689.1"
                     /translation="MAAELRSTFEGRTMVLTISNPEHRNALGPEMYAAGVEALNVAES
                     NADVHCVVLTGEGEAFCAGGNLQRLQANRQKPPEVQAQSIENLHSWIEAIRTFPKPVI
                     AAVEGPAAGAGFSLALACDLIVAARNAVFVMAYSSVALSPDGGGSWSLAHALPRQLVN
                     ELLMGGERMGAQRLHELGVVNRLCDAGQALGTALDWAEKLAARAPNVLTSIKELVSEA
                     PAHSLNAHLAMERDHFVTNLHHANGGIGIAAFLAKQTPRYE"
     gene            complement(1611..1841)
                     /locus_tag="PKV30_05410"
     CDS             complement(1611..1841)
                     /locus_tag="PKV30_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29690.1"
                     /translation="MTAAPPPAGKPQGIIADPNALLQVEIDRVVLDQSGRSAEELQKL
                     AELQDLPPLSDEELARQAEADPGADGDASTPE"
     gene            complement(1928..3451)
                     /locus_tag="PKV30_05415"
     CDS             complement(1928..3451)
                     /locus_tag="PKV30_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRL29691.1"
                     /translation="METPEFKTVGIVGAGAMGRGIAQIAAQAGSKVLLFDTQADAVAK
                     ARESVAAQWDKLVEKGRIDAAVAAAQKERLQPAATLADLAPCDLIVEAIVERLDVKKG
                     LFAELEGIVQTTAVLATNTSSLSVTAIAAGLKRPQQFAGFHFFNPVPLMKVVECIAGL
                     KTDPAVCQRLATYARQMGHTPVQAEDTPGFIVNHAGRGYGTEALRVVSEGVADFATID
                     RILKDQVGFKLGPFELMDLTALDVSHPVMESIYRQYYDEPRYRPSVITAQRLAGGVVG
                     KKVGDGFYKYDGGAAQIASEPPVPQVDEMPPVWVSTRAARRAELYQLLKDLGAKIETG
                     QSPSLNALTLVAPLGFDVTTVAVVERLDPARTIGIDMLIDDAATKRRVLATNPATRAD
                     MRDAAHALFARDGKPVSVVRDSGGFVTQRVVSTIVNIAADMCQQRVCSPKDLETAVTL
                     GLGYPLGPLAMGDKFGPANILEVLFNMQTVYGDPRYRPSPWLRRRGAIGLSLMHEEA"
     gene            complement(3909..4916)
                     /locus_tag="PKV30_05420"
     CDS             complement(3909..4916)
                     /locus_tag="PKV30_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HRL29692.1"
                     /translation="MNIVILDDYQDAVRKLAAASKLEAYPAKVYTNTVKGLGQLSVRL
                     RDADVIVLIRERTQLTRALIEKLPRLKMVSQTGRIGSHVDVTACTERGIAVAEGVGSP
                     VAAAELTWALIMAAARRLPQYIGNLKHGAWQQSGLKAASMPPNFGLGTVLRGRTLGIW
                     GYGKIGQLVAGFGRAFGMQVMVWGSPDSMSRARADGHHAAASRADFFAFSDVLSLHLR
                     LADSTRGLVKLEDLSRMKPTALLVNTSRAELIEPDALVSALNRGRPGMAAVDVFETEP
                     ILQGHALLRLENCICTPHIGYVEQDSYELYFGAAFDNVINYLRGTPTNIVNPGALQVR
                     R"
     gene            complement(5091..5552)
                     /locus_tag="PKV30_05425"
     CDS             complement(5091..5552)
                     /locus_tag="PKV30_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29693.1"
                     /translation="MGNNVIYLADVAVPSYRRTRAPIQGAVRSATPSDSGWIEAARRR
                     ATQKRMREIASPVEKVAQRPMLSEGSDAAALDSAGRSRAEMPNARAVVDQAVAQNHPA
                     SSTLRVTHLPDAWRPAGTSGRLRISGRLIDVCAELDRLAAAEAQAALPRRA"
     gene            complement(5714..6379)
                     /gene="lexA"
                     /locus_tag="PKV30_05430"
     CDS             complement(5714..6379)
                     /gene="lexA"
                     /locus_tag="PKV30_05430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRL29694.1"
                     /translation="MTDSPKLTARQHQILQLVQHAIARTGAPPTRAEIAAELGFKSAN
                     AAEEHLQALARKGVIELVSGTSRGIRLKGAGRQGLRGDAFSMSLPGMAQLALPLVGRV
                     AAGSPILAQEHVDQTYHLEPSLFAERPDYLLRVRGMSMRDAGIMDGDLLAVQSARDAR
                     NGQIVVARLGDDVTVKRFHRHKNQIELHAENPDYPTIVVQPGDPFEIEGRAVGLIRNS
                     MLM"
     gene            complement(6524..6841)
                     /locus_tag="PKV30_05435"
     CDS             complement(6524..6841)
                     /locus_tag="PKV30_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL29695.1"
                     /translation="MLRGHCSNEVVAAQFGVHRHTLYRRLAAAGCTYEQLLEQSRQRL
                     ATRLLQETDMPVSEIASMLGYAAQGNFTRAFARWFGRSPSAWRKMPPTNAGRKGIRGK
                     ASR"
     gene            complement(6971..7579)
                     /locus_tag="PKV30_05440"
                     /pseudo
     CDS             complement(6971..7579)
                     /locus_tag="PKV30_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
     gene            7642..8325
                     /locus_tag="PKV30_05445"
     CDS             7642..8325
                     /locus_tag="PKV30_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL29696.1"
                     /translation="MAADLKGRVALVTGVGPGLGQGIAVALARAGASVVVCDVNDEAV
                     AATRKDVEADGAECLALRCDVSDSQAVREMFDAARARFGSLHVLVNNAALTPNRPGDT
                     ERRNRLYAYNSTPVPRDTLNFAAEITDEEWHRYWGVNVQGVFYCTREALKLMQPQRYG
                     RIVNAASIAGLSAKSAHSPHYSATKGAVHRLYALGRARSGRRRHPGQRHGAGRRRHAG
                     LPGLSGRQR"
     gene            8267..8470
                     /locus_tag="PKV30_05450"
     CDS             8267..8470
                     /locus_tag="PKV30_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29697.1"
                     /translation="MAPGGVATPAFLGYLDASGEEARNRLWQGCPTGRFGTIEEYVAT
                     VLHLAGEHYMTGQVLSPKGGSVI"
     gene            8486..>8932
                     /locus_tag="PKV30_05455"
     CDS             8486..>8932
                     /locus_tag="PKV30_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL29698.1"
                     /translation="MNLQDLDRLHASDPAWADAWGALAARSTGDSLDPKLAALIRLNI
                     DVTATHLYAPGVRRHIRACIEAGATRAEILAVFKLAAVIGIHALALGVPALEAELKAR
                     NVPNEALPAPATPVTDAVRARGQFNPLWETLSQWDPVWLERFLAMGL"
BASE COUNT         1413 a         3010 c         3005 g         1504 t
ORIGIN      
        1 gtcactcttc gccgatgcgc aacagcgcat cgcgatcgca caccaccagc cccgtcggct
       61 cgacgcggat cacgccctcg cgctccatcg acttcagctc ctggttcacg cgctggcgcg
      121 aggcgcccag caactgcgcc agctcttcct gcgccagttg cagggtgatg cgccattcgt
      181 cgttggacga caggctgggc acgccgtagc tgcgcaccag gtgccccaac tgctttgcca
      241 ggcgcgcgcg cagcggcagc gtgttgaggt cttccacctg cccgaacagc aggcgaatgc
      301 ggcgcgcctg cagcttgagc agcgcgtcgt acagctcggg gtgctgcgcc agaatcttgt
      361 ggaagtcggc gcgcgccacg cacaggatgg tggtcgcgcc atgcgcatac gcatcgtgcg
      421 tgcgccggtc gccgtcgaag atggccacgt cgccaaacca gatgcccggc tccacatagg
      481 tcagcgtgat ctgcttgccc gtgagcgtgg tcgagctgac gcgcaccgcg cccttggcgc
      541 acgcaatcca ttgttccgcg ggctcgccgc gggccgcgat cagatcgcca tcggggtagc
      601 gtttgacgta ggcgcatcga agaatgtcgt gccgcagtga aggggaaagt gaggaaaacc
      661 agcgaccggt gttgatcgcc tcgcgttcct ccatggtaag aatggggtcg tccatgggct
      721 gtcttatggg tgacttaaaa agcgcgcact gtctccggcg ggacggcggc acgctgattg
      781 tgacttgacc aggcggccgc ccgccagcgc gcggcctatt cgtagcgcgg tgtctgcttg
      841 gccagaaaag cggcgatgcc gatgccgccg ttggcgtggt gcaggttggt cacgaagtga
      901 tcgcgctcca tcgccaggtg cgcgttcagg ctgtgggcgg gcgcctcgct gaccagttcc
      961 ttgatgctgg tcagcacgtt gggcgcgcgg gcggccagct tttcggccca gtcgagcgcg
     1021 gtgcccaagg cctggcccgc atcgcacagg cggttgacca cgcccagctc gtgcaggcgc
     1081 tgcgcgccca tgcgctcgcc gcccatcagc agctcattga ccaactggcg cggcagcgcg
     1141 tgcgccaggc tccagctgcc gccgccgtcg ggcgacagcg cgacgctgct gtaggccatg
     1201 acgaacaccg cgttgcgcgc cgccacgatc aggtcgcacg ccagcgcgag cgaaaaaccg
     1261 gccccggcgg ccggcccctc gaccgccgcg atgacgggct tggggaaggt gcggatggcc
     1321 tcgatccagc tgtgcaggtt ttcgatgctt tgcgcctgca cctcgggcgg cttttgccgg
     1381 ttggcctgca ggcgctgcag gttgccgccg gcgcagaagg cctcgccctc gcccgtcagc
     1441 acgacgcaat gcacgtcggc gttggattcg gcgacgttca gcgcctcgac gccggccgcg
     1501 tacatctcgg gcccgagcgc gttgcggtgc tcggggttgc tgatagtcag caccatggtg
     1561 cgaccttcga aggtgctgcg cagctcggcg gccatgcgtt ctcgccgcgt ttattcgggc
     1621 gtggaggcgt cgccgtcggc gcccgggtcg gcttcggcct ggcgcgccag ttcttcgtcg
     1681 ctgagcggcg gcaggtcttg cagctcggcc agcttttgca gctcttcggc actgcggccg
     1741 gattgatcga gcaccacgcg gtcgatctcg acctgcagca gcgcgttggg atcggcaatg
     1801 atgccttgcg gcttgccggc gggcggtggg gcggcggtca tggcgtctcc tgttactcta
     1861 tttttgatag ctgcctgcgc ttgtccatca agcgctgaag gctgatttca tcattgaatc
     1921 cgcgtggtca agcctcttcg tgcatcaggc tcagcccgat ggcgccgcgc cggcgcagcc
     1981 aagggctggg acgatagcgc ggatcgccat acacggtctg catattgaac agcacttcaa
     2041 gaatgttggc cggcccgaac ttgtcgccca tggccagcgg gcccagcgga taacccaggc
     2101 ccagggtgac ggcggtctcc agatccttgg ggctgcacac gcgctgctgg cacatgtcgg
     2161 cggcaatgtt gactattgtt gaaaccacgc gctgggtgac aaagccgccc gagtcgcgga
     2221 ccacgctcac cggcttgccg tcgcgcgcga acagcgcgtg cgccgcatcg cgcatgtcgg
     2281 cgcgcgtggc cgggttggtg gccagcacgc ggcgcttggt ggccgcgtcg tcgatcagca
     2341 tgtcgatgcc gatggtgcgc gccgggtcga gccgctcgac cacggccacg gtggtgacgt
     2401 caaaacccag cggcgccacc aacgtgagcg cgttcagcga cggtgactgg ccggtctcga
     2461 tcttcgcgcc caagtccttc agcagctggt acagctcggc gcggcgcgcg gcgcgggtgc
     2521 tgacccacac gggcggcatt tcatcgacct gcggcacggg cggctcagac gcgatctgcg
     2581 cggcgccgcc gtcgtacttg taaaagccgt cgcccacctt cttgcccacc acgccgccgg
     2641 ccaggcgctg cgcggtgatg acgctggggc ggtagcgcgg ctcgtcgtag tactggcggt
     2701 agatcgactc catcaccggg tgcgaaacgt cgagcgcggt caggtccatc agctcgaacg
     2761 gccccagctt gaagccgacc tggtctttca gaatacggtc aatggtggcg aagtcggcca
     2821 cgccctcact cacgacgcgc aacgcctcgg tgccgtagcc gcggccagcg tggttgacga
     2881 tgaagcccgg cgtgtcctcg gcctgcaccg gcgtgtggcc catctggcgc gcataggtcg
     2941 ccaagcgctg gcacacggcg gggtcggtct tcaggccagc gatgcattcg accaccttca
     3001 tcaggggcac cgggttgaag aagtgaaagc ccgcgaactg ctgcggccgc ttcaggcccg
     3061 ccgcgatggc cgtgaccgag agcgacgagg tgttggtggc cagcaccgcc gtggtctgca
     3121 cgatgccttc aagttcggcg aacagtcctt tcttgacgtc gagccgctcg acgatggctt
     3181 cgacgatcag gtcgcacggc gccagatcgg ccagcgtggc ggcaggctgc aggcgctctt
     3241 tctgcgcggc ggccacggcg gcgtcgatgc ggcctttctc gaccagcttg tcccattgcg
     3301 cggccaccga ttcgcgcgcc ttggccacgg catcggcctg cgtgtcgaac agcaggacct
     3361 tgctgcccgc ctgcgccgcg atctgggcaa tgccccggcc catggcgccg gcacccacga
     3421 tgccgacggt tttgaattca ggtgtctcca ttcggatgat tatggtgcag gtggcggggt
     3481 gcaagggccc gggcatgaac gccctgctca gcgcttgcag gcggttggcg ggcgtcgtgt
     3541 catcgcgcga tcgctttcat ttcgatagct gctcgcgctt tctctgcggg cgctgaacgc
     3601 tcatttgcct tgaaatggga aggctgccgg gcaggccgcg acgcgccgtc gcattcgcgg
     3661 ccaggtccga cgccgctaac ttcaaaatcg cttgatggca agaagtctca gacgacgcat
     3721 gcgcctttgg ctccgtcatc agccttgtgg gcgcgggctt gcccgcgatg gagcgccctt
     3781 gggccacacc ggctccatcg cggccaaggc cgctccgcac agcatccgag acttcttgcc
     3841 ggcaatcaaa acgatagctg gaggcgcttt ctgcacaagc gctggcagcc caaatcgctc
     3901 ttggacggtc aacgccgaac ctgcagcgcc cccggattga cgatgttggt cggcgtgccg
     3961 cgcaggtagt tgatgacgtt gtcgaacgcg gcgccaaagt acagctcgta gctgtcctgc
     4021 tccacgtagc cgatgtgggg cgtgcagatg cagttctcca gccgcagcag cgcgtggccc
     4081 tgcaggatcg gctctgtctc gaacacatcg accgccgcca tgccgggccg gccgcgattg
     4141 agcgcactga ccagcgcatc gggctcgatc agctcggcac gcgaggtgtt gaccagcagc
     4201 gccgtcggct tcatgcggct cagatcctcc agcttgacca ggccgcgcgt actgtcggcc
     4261 agtcgcagat gcagcgacag cacgtcggag aaagcaaaga aatcggcgcg cgaggcggcg
     4321 gcatggtgcc cgtcggcgcg cgcgcgcgac atcgaatcgg gcgagcccca gaccatgacc
     4381 tgcatgccga acgcccgccc aaagccggcc accagttggc cgatcttgcc gtagccccag
     4441 atgccgagcg tgcgcccgcg caacaccgtg ccaagcccga agttgggcgg catcgacgcc
     4501 gccttcaacc ccgactgctg ccacgcgccg tgcttgaggt tgccgatgta ttgcggcagc
     4561 cggcgcgccg ccgccatgat gagtgcccac gtcaactcgg ccgccgccac cggcgagccc
     4621 acgccttcgg ccaccgcaat gccgcgctcg gtgcaggcgg tgacgtcgac atggctgccg
     4681 atgcggccgg tctgcgacac catcttcagg cgcggcagct tctcgatcag cgcccgcgtg
     4741 agttgcgtgc gctcgcggat cagcacaatg acgtcggcgt cgcgcaaacg caccgacaac
     4801 tgaccgagac ccttgacggt gttggtgtac accttggctg gataggcttc cagcttggac
     4861 gcggccgcca gcttgcgcac ggcgtcctga tagtcgtcga ggatcacgat gttcatgccg
     4921 gcattgtgcc tttggcgcca cgccgcgcgg catggagccc tgcagtgaac cccggtcttg
     4981 gctggcggcg cccgtccatc ggcctgaaac cgatgcagcc atcgacggcc tgggcggcac
     5041 gccgcagaca acgccatcgc ccacgacgcg cccgaaccta tgaccgaacc tcaggctcgg
     5101 cgcggcagtg cggcctgcgc ttcggccgcc gccaggcgat caagttcagc acagacgtcg
     5161 atgagtcgcc ccgagatgcg caggcgcccg ctggtgcccg cggggcgcca ggcgtcgggc
     5221 aggtgcgtca cgcgcagcgt ggacgacgcg gggtgatttt gcgcaaccgc ctggtcgacg
     5281 acggcgcgcg cattcggcat ttctgccctc gaccggcctg ccgaatcaag cgctgcggcg
     5341 tcgctacctt cggacagcat tggccgttgc gctacctttt ccaccggcga agcaatctcg
     5401 cgcatgcgct tttgcgtcgc ccgtcgccgc gctgcctcga tccagccgct gtcggacggc
     5461 gtggccgaac gcacagcgcc ttgaatcggg gcacgagtgc ggcgatagct gggcacggcc
     5521 acatcggcca ggtaaatgac gttgtttccc atgtaaaacc ttcagaagaa gtgaggttgg
     5581 acacaggcag gattgagatg accagcgcgc agagcgcatg gccttgagcg ccgtcgcagg
     5641 gtgcgcacga cgggctcggc tggcgccgac cgccgactgc acgcccgcca cccgcggcgg
     5701 cgcggacgtg gcgctacatg agcatgctgt tgcggatgag gcccacggcg cggccttcga
     5761 tctcgaacgg gtcgcccggc tgaaccacga tggtggggta atcggggttc tcggcgtgca
     5821 gttcgatctg gttcttgtgc cgatgaaagc gcttgaccgt gacgtcatcg cccaggcggg
     5881 ccaccacgat ctggccgttg cgggcgtcgc gtgcagattg cacggccagc aggtcgccat
     5941 ccatgatgcc ggcgtcacgc atcgacatgc cgcgcacccg aagcaggtag tctggccgtt
     6001 cggcgaacag gctcggctcg aggtggtaag tctgatcgac gtgttcttgc gccaggatgg
     6061 gcgagccggc agccacccgg cccaccaagg gcagcgccag ttgcgccatg cccggcagcg
     6121 acatgctgaa agcgtcaccc cgaagcccct ggcgacccgc gcccttcaag cggatgccgc
     6181 gcgaggtacc gctcaccagt tcaatcacgc ccttgcgtgc cagggcctgc agatgctctt
     6241 cggccgcgtt ggccgacttg aaacccagct cggccgcgat ctcggcgcgc gtgggtggcg
     6301 cgccagtgcg cgcaatcgcg tgctgcacca attgcagaat ctgatgttgg cgagcggtaa
     6361 gcttggggct gtcggtcatg gcgtcctccg gctatcgggt tcgtcgaaaa ctgtgttgat
     6421 atccagttac tgtattttta cccagttttc gacagcatgc aagcgccaac acgccgtttt
     6481 ttcttcggcc gtcgctactc gggccaggcg ccccttcccg cgctcacctg ctggccttcc
     6541 cgcgaattcc tttgcggcca gcgttcgtcg gcggcatctt tcgccacgca ctcggcgagc
     6601 ggccgaacca gcgcgcaaac gcgcgcgtga aattgccctg cgccgcgtag cccagcatcg
     6661 aggcgatctc tgacaccggc atgtcggttt cctgcagcag gcgcgtggcc agtcgttggc
     6721 gcgattgctc cagcaactgc tcgtaggtgc agccggctgc cgccaggcgc cgatacagcg
     6781 tgtggcgatg cacgccgaac tgggcggcaa ccacttcatt gctgcaatgg ccgcgcagca
     6841 tgcgggccgc gatggcgtgt tgcacctgac ccgcaaagtc ggtcggctgg tcggtttcca
     6901 gcgcctgcaa ctgctttttc aagaacgcgg cgagttgcgg gtctgcactc tctcgtgcgc
     6961 gatctaagtc ggcgtcctga aaaagcacct catggcgcgg ctggtcgaac aacaccggcg
     7021 cgcggaagaa cgcggtgtag ggcgcaaagt ccgccggccg gcgctgcgcc atgcgcacaa
     7081 gggccgggcg ccattcggtg ccgaagactt gcgccaggtt gcgcacgccg ccggccagaa
     7141 aggcggtcat caagggctgg ctgcccgcaa agcgcgcatc ggttttgtag gccagcacgg
     7201 cgtagccctg gtcgcttttc agcgtcaggc agatgccgcg gtaccagatg cgggcttagc
     7261 gcagcagtct ggccagcgcc tcgcccgccg tgggcgcatt gagcgccagc gcgcgcaccg
     7321 ggcccacgtt ttcgagacga gcctgcgcgc ccagcaacag cggcaggtgc tcgatgccac
     7381 tggtgtcgat ggcgtcctga accacccggc catgcaaatg aatcggcgac ggtgccaagg
     7441 ggtcgctcaa cgtcctgaca ccgacgccgt ggcggcgcag cagcgcgtcg gcatcgtggc
     7501 cgtgcttgcg caacacgtcc gccacgaccg acaaggtgcc gatcggcagc gaatcggtgt
     7561 gacggtcagc gctgggcatg gcgcccaacc gcaagctggg gcagatcggc ttgcccgtgc
     7621 ctggcatggc gctggccaga gatggcggcc gatctcaaag ggcgcgtcgc cctggtgacc
     7681 ggcgtcggac cggggctcgg ccagggcatc gcggtcgcgt tggcccgcgc cggggccagc
     7741 gtggtggtgt gcgatgtcaa cgacgaagcc gtcgccgcca cgcgcaagga cgtcgaggcc
     7801 gatggcgccg agtgcctggc gctgcgctgc gacgtgtcgg acagccaggc ggtgcgcgag
     7861 atgttcgacg ccgcccgggc gcgctttggc agcttgcacg tcctggtcaa caacgccgcg
     7921 ctcacgccca accgcccggg ggacaccgaa cggcgcaacc ggctgtatgc ctacaacagc
     7981 acgccagtgc cgcgcgacac gctgaacttc gccgccgaaa tcaccgatga agagtggcac
     8041 cgctactggg gcgtgaacgt gcagggcgtg ttctattgca cgagggaggc gctcaagctg
     8101 atgcagccgc agcgctatgg gcgcatcgtc aacgcggcct ccatcgccgg tctgtcggcc
     8161 aagagcgcgc acagcccgca ctacagcgcg accaaggggg cggtccatcg cctttacgcg
     8221 ctcggtcgcg cacgaagtgg ccggcgccgg catcctggtc aacgccatgg cgccgggcgg
     8281 cgtcgccacg ccggccttcc tgggctatct ggacgccagc ggtgaagaag cgcgcaaccg
     8341 gttgtggcaa ggctgcccga cggggcgctt cggcaccatc gaagaatacg tcgccaccgt
     8401 gctgcacctg gccggcgagc actacatgac cgggcaggtg ctcagcccca aaggcggttc
     8461 ggtgatctga acagcgagga cgaccatgaa ccttcaagac cttgaccgcc tgcacgccag
     8521 cgaccctgcc tgggccgatg catggggcgc gctcgccgcg cgcagcaccg gcgattcgct
     8581 cgaccccaag ctggctgcat tgattcgcct gaacatcgac gtgacggcca cgcacctgta
     8641 tgcgccgggc gtacggcgtc acatccgtgc gtgcattgaa gcgggcgcca cacgggccga
     8701 gattctggcc gtgttcaagc tcgcggcggt gatcggcatt cacgccctgg cgctcggcgt
     8761 gcccgcgctg gaggccgagc tgaaagcccg caacgtgccg aacgaagcgc tgcccgctcc
     8821 agcgacaccc gtcaccgacg cggtgcgcgc acgcggccag ttcaacccgc tgtgggaaac
     8881 gctgtcgcag tgggacccgg tctggctcga acgctttctg gccatgggct tg
//
LOCUS       DAOMVO010000076         8927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2004_length_8927_cov_11.752705, whole genome shotgun sequence.
ACCESSION   DAOMVO010000076 DAOMVO010000000
VERSION     DAOMVO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_8927_cov_11.752705"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKV30_05460"
     CDS             <1..183
                     /locus_tag="PKV30_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29699.1"
                     /translation="ADMTVKMVVPTLGRREGKAGVVGLMSMEGEKRKGQPVWREVQDK
                     KGRWRLRDKREQLSIL"
     gene            complement(191..1132)
                     /locus_tag="PKV30_05465"
     CDS             complement(191..1132)
                     /locus_tag="PKV30_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HRL29700.1"
                     /translation="MNLHQFRFVQEAARRGLNLTEAAKALHTSQPGVSKAIIELEEEL
                     GIDIFTRHGKRLKRITEPGQHVLDSIAIILREVGNLKRIGEQYSAQDSGTLSIATTHT
                     QARYVLPVPVAKLRERYPKVNISLHQGAPDQVARMVIEETAEIGMATESLANYPELVT
                     LPCYEWQHVLVVPATHALAKKERLTLEDIAAEPLITYHPSFTGRTRIDTAFAQRNLAP
                     RIVLEAIDSDVIKTYVRLGLGIGIVAEMAVDGDKDPDLVTRPLGQLLGKNVARIALKR
                     GAYLRDFVYHFASLLSDRLDKDLILKAMNGHVADYEL"
     gene            complement(1177..1566)
                     /locus_tag="PKV30_05470"
     CDS             complement(1177..1566)
                     /locus_tag="PKV30_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRL29701.1"
                     /translation="MADLTASDTGIVLFAHGSRDAAWRAPIEAVAARMREMAPGTRVQ
                     CAYLEYTAPDLGTTVAALLAEGARAITVWPMFLGTGRHARDDLPRLLAELREQHPGVA
                     FNLREAIAEHPDVLQAMAVAALNRPAP"
     gene            complement(1568..2677)
                     /gene="lptG"
                     /locus_tag="PKV30_05475"
     CDS             complement(1568..2677)
                     /gene="lptG"
                     /locus_tag="PKV30_05475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRL29702.1"
                     /translation="MKTIRRLIYSEVLRAVGFVTLAFLALFFFFDLVDALGDVRDGAA
                     RGYTMGYALLVVLLQVPNHLYELMPIAVLIGTIFVMARLAASSEFTILRTSGLGPMRA
                     LGTLLLLGAAFVALTFVFGDYVAPAANRAAQTLQARFLGDITVGRTGAWLKDREPTRN
                     YSVNVGALGSDGTLNHVRIYEFADATGRLLSTTKAEKGQIVDGGWQLQGVERRSFEQA
                     GPQAPLKMQVTRQDQWQWPTDLALDTVAAALLSPDRMQTLDMFQYIRHLQANGQSAQR
                     YEIEFWRKVFYPISCLVMMVLALPFAYLHFRAGNITGYVFIGVLVGISFFLLNNVFGH
                     IGNLRDWSPWIAAASPGIIYSLLSLALFGWLVLRQ"
     gene            complement(2674..3774)
                     /gene="lptF"
                     /locus_tag="PKV30_05480"
     CDS             complement(2674..3774)
                     /gene="lptF"
                     /locus_tag="PKV30_05480"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRL29703.1"
                     /translation="MLFDSSLRKELGRSFGATLVVLITIVMTVMLIRTLGQATRGNVN
                     PSEVMLVMGFMVLGHLPTILTLSLFIAITATLTRMYASSEMVVWFSSGQGLWSFLKPT
                     LRFAWPVLLSVAVLALVVWPWSNQQARELRDRYQGRGDIERVSPGRFIESAGGQRVFF
                     IDKDTPDANTGSNIFIATTERGKETVTSAQNGRLETIKGQRFIILDNGQRLETELATG
                     QTRVSEFAEYGNRISSSTTGPGPRQQPNLTPTMTLLRQPTPLHLGELSWRLGLALAAI
                     NCVIIALAATRVNPRVGRSAGLVFALFAFASYYNLLTVGESWIRNGRIGFVPYVLLLH
                     GGIFLLAMGWLLARHNGWFIGWPLRPARRSGA"
     gene            3799..5331
                     /locus_tag="PKV30_05485"
     CDS             3799..5331
                     /locus_tag="PKV30_05485"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRL29704.1"
                     /translation="MDFRLDSLILAEAAATKSPALMVLVADGFEPGADALSALIQRAL
                     GSGDFKTEAGALLSSYGAARVTAERVVLVGVGDASGPQVRKAVSAGVLALRGGPAKSL
                     VLCFGQPADAAAVRAATLAVAESCYAYGATKGKKNQKDQKKAPPRLTDVLIGVPDAEP
                     VRDAFRIGVGIASGVELAREWANRPANHATPTMLGEAARDLADLPRVDCEVLGPKEVA
                     KLGMGAFQAVAQGSKEELRFIVLRYHGAPKSEAPVVLVGKGITFDTGGISLKPAPEMD
                     EMKYDMSGAASVLGTFRALAELKPAINVVGLIPACENMPDGGAIKPGDVVTSLSGQTI
                     EILNTDAEGRLVLCDALTYAERFKPRAVIDIATLTGACVIALGGVRSGLFSPDDALAD
                     ALLAAGDRALDLCWRLPLDADYAEALKTHFADVANVGGRPAGAVTAAKFLQRFATEYP
                     WAHLDIAGTAWKGGAAKGATGRPVGLLVQYLLAQAGPDTAKPSGKAKSAKKAKSTKAR
                     RA"
     gene            5520..5948
                     /locus_tag="PKV30_05490"
     CDS             5520..5948
                     /locus_tag="PKV30_05490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRL29705.1"
                     /translation="MTHIGFHFNMPDKLAYACRLLRKAYAAGGPVGVVGPAPAIEALD
                     TELWRFSALDFIPHCLASAPEAMRVFTPIVLAQDCADLPQTGVLVHLGEQVPAGFERF
                     ERLIELVDTSDADRSAARQRWRHYADRGYPIQRHDLAAKE"
     gene            5954..6331
                     /locus_tag="PKV30_05495"
     CDS             5954..6331
                     /locus_tag="PKV30_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29706.1"
                     /translation="MSTTEDDAQPRSAPRFVPTLTEVVGAADASAPAAAPSVPAPSTP
                     VAPPPMPSIGAATAAHTADAMLARIGPDLDRQIAEAIGRVLHEQLLGFNGRVQKAVAE
                     VVREAVAKALAQDAHGRDAGTNP"
     gene            6587..7642
                     /locus_tag="PKV30_05500"
     CDS             6587..7642
                     /locus_tag="PKV30_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL29707.1"
                     /translation="MVVRIGHVGPVSGPQAHYGKDNENGSRMAIEDLNTQNIVIGGKK
                     VKFEIQAEDDAADPKQGTAAAQKLCDAKVNGVVGHLNSGTTIPASKVYNDCGIPHVTG
                     AATNPDLTKPGYNTTYRIIANDLALGAGLANYAADDLKLKKIAVIDDRTAYGQGVAKV
                     FAETAKAKGIQIVDEQFTTDKATDFMAILTAIKAKGPDGVFFGGMDPQAGPMLRQMEQ
                     LGMNNVKYFGGDGICTSEIAKLAAGAKTLGNVICAEGGASIQKMPGGTEWKARYDAKY
                     PNQFQVYSPYTYDATMLLADAMKRADSADPKVYIPKLIESNYKGVTANIQFEPNGEMK
                     NAAITLYKYVDGKKTPL"
     gene            complement(7700..>8927)
                     /gene="mnmC"
                     /locus_tag="PKV30_05505"
     CDS             complement(7700..>8927)
                     /gene="mnmC"
                     /locus_tag="PKV30_05505"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HRL29708.1"
                     /translation="LAGAAVARALALRGWQVTVLDSAAHPAAGASGLPAGVVAAHVSP
                     DDRPLSRLTRAGARATLATARDLLQDGIDFGATGVLERHAPGERRLPADWAAAIDSEA
                     AGAGVASAETQITQEKAAQAGVPLNAAERALWHTHAGWLRPAALVRAMLQTHGITWRG
                     ASPVARVTADGARWRLADTAGALLAEAELVVITAGFDSLGLLQASGDQDASALPLHAL
                     RGQVAFGPMPGGAADEALPRFPVNGHGSLIAHVPGADGPLWVIGSTFERGNRRADVLP
                     ADHQHNLQRLTELLPRAANALAPQWVDGRAGAWAGVRATLPDRLPAVGAWSVHEQNQA
                     PALDGQAPAAINSEVSTPPLPVHLLTGLGARGLTLALLCADVLTAGLHGEPLPVERSL
                     ARRLRAGRWLTRHPGS"
BASE COUNT         1546 a         2930 c         3100 g         1351 t
ORIGIN      
        1 gccgacatga cggtgaagat ggtggtgccg acgttgggca ggcgcgaggg gaaggcgggg
       61 gtggtggggc tcatgtcgat ggagggcgag aagagaaagg ggcagccggt ctggcgggag
      121 gttcaagata aaaaaggccg ctggcgcttg agggacaagc gcgaacagct atcaatacta
      181 tagtcgtcga tcacagctcg tagtcggcca cgtgcccgtt catggccttc aggatcaagt
      241 ccttgtcgag cctatccgac agcaggctgg cgaagtgata gacaaagtcg cgcaggtacg
      301 caccgcgctt gagggcgatg cgcgcgacgt tcttgcccag cagctggccg agcgggcggg
      361 tgaccagatc ggggtctttg tcgccatcga cggccatttc ggccacgatg ccgatgccga
      421 ggcccagtcg cacataggtc ttgatgacgt ccgaatcaat cgcctccagc acgatgcgcg
      481 gcgccaggtt gcgttgggca aaggcggtgt cgatgcgcgt gcggccggtg aaggaggggt
      541 ggtaggtaat aagtggctcc gccgcaatgt cctccagcgt cagacgctct ttttttgcga
      601 gcgcatgggt cgcgggcacg accagcacgt gttgccattc gtagcagggc agggtgacga
      661 gttcggggta gttggccagc gattcggtgg ccatgccgat ttcggccgtc tcttcgatca
      721 ccatgcgcgc cacctggtcg ggcgcgccct ggtgcaggct gatgttgacc ttggggtagc
      781 gctcgcgcag cttggccacg ggcacgggca gcacgtagcg cgcctgggtg tgcgtggtgg
      841 caatcgacag ggtgccgctg tcttgcgcgc tgtattgctc gccgatgcgc tttaggttgc
      901 cgacttcgcg caggatgatg gcgatgctgt ccagcacatg ctggccgggc tcggtgatgc
      961 gcttgaggcg cttgccgtgg cgggtgaaga tgtcgatgcc gagttcttct tccagctcga
     1021 tgatggcctt ggagacaccg ggttgcgagg tgtgcagcgc cttggccgct tcagtcaggt
     1081 tcaggccccg gcgcgcggct tcctggacaa agcggaattg gtgcaggttc atggcgtttc
     1141 ttgaatatga agcgccgata ttatggcaaa tggtgtctaa ggcgcaggac gattcagcgc
     1201 cgccacggcc atggcttgca gcacgtcggg gtgctcggca atcgcctcgc gcaggttgaa
     1261 ggcgacgccg ggatgttgct cgcgcaactc ggccagcagg cgcggcaaat cgtcgcgcgc
     1321 gtggcgaccg gtgcccagaa acatcggcca gacggtgatg gcgcgggcgc cttcggccag
     1381 cagcgcggcc acggtggtgc ccaggtcggg tgcggtgtat tcgaggtaag cgcactgcac
     1441 gcgcgtgccg ggcgccattt cgcgcatgcg cgcggccacc gcctcaatgg gcgcgcgcca
     1501 ggccgcatcg cgcgagccgt gcgcgaacag cacgatgccg gtgtctgagg cggtcaaatc
     1561 ggccacgcta ttgcctcagc accagccagc cgaacagcgc cagcgacaac agcgaataaa
     1621 tgatgccggg cgaggccgcc gcgatccagg gcgaccagtc gcgcaggttg ccgatgtggc
     1681 cgaacacgtt gttgagcaga aagaagctga tgcccaccag cacgccgatg aacacatagc
     1741 ccgtaatgtt tccggcgcgg aaatgcaaat aggcaaacgg cagcgccagc accatcatca
     1801 ccaggcagct gatggggtag aacactttgc gccagaactc gatttcatac cgctgcgcgc
     1861 tctgcccgtt ggcctgcagg tggcgaatgt actggaacat gtccagcgtc tgcatgcggt
     1921 cggggctgag cagcgcggcg gccacggtgt cgagcgccag atcggttggc cactgccact
     1981 ggtcctgccg cgtgacctgc atcttgagcg gggcctgcgg gccggcctgc tcgaaactgc
     2041 ggcgctcgac gccttgcagt tgccagccgc catcgacgat ctggcctttc tcagccttgg
     2101 tggtcgagag caggcgcccg gtggcgtcgg caaattcgta gatgcgcacg tggttcagcg
     2161 tgccgtccga acccagcgcg cccacgttga ccgaatagtt gcgcgtcggt tcgcggtctt
     2221 tcagccaggc gccggtgcgg ccgacggtga tgtcgcccag aaagcgcgcc tgcagcgtct
     2281 gcgccgcgcg gttggcggcc ggcgccacgt agtcgccaaa cacgaaggtc agcgccacaa
     2341 aggcggcgcc cagcagcagc agcgtgccca gcgcgcgcat gggccccagg ccgctggtgc
     2401 gcaggatggt gaactcagag ctggcggcca ggcgtgccat gacgaagatg gtgccgatca
     2461 gcaccgcgat cggcatcagc tcgtacaaat ggttgggcac ctgcagcagc acgaccagca
     2521 gcgcgtagcc catggtgtag ccgcgcgccg cgccgtcgcg cacgtcgccc agcgcgtcga
     2581 ccagatcgaa gaagaagaac agcgccagaa acgcgagcgt gacgaagccg accgcgcgca
     2641 gcacctcgga atagatcagg cggcgaatgg tcttcatgca ccactccggc gcgccgggcg
     2701 cagcggccaa ccaatgaacc agccgttgtg ccgcgccagc agccagccca tggccagcag
     2761 aaagatgccg ccgtgcagca gtagcacgta gggcacgaag ccgatgcggc cgttgcggat
     2821 ccagctttcg cccaccgtga gcaggttgta gtagctggca aaggcaaaca gcgcaaacac
     2881 caggccggcg ctgcgcccga cgcgcggatt cacccgcgtg gcggccagcg cgatgatcac
     2941 gcagttgatg gccgccaaag caagaccgag gcgccaggac aattcgccca ggtgcagcgg
     3001 cgttggctgg cgcagcaggg tcatcgtcgg tgtcaggttg ggctgctggc gcgggccggg
     3061 gccggtggtg ctggagctga tgcggttgcc gtattcggca aactcgctca cccgcgtctg
     3121 gccggtggcc agttcggtct cgagccgctg gccgttgtcg aggatgatga agcgctggcc
     3181 cttgatggtt tcgagccggc cgttctgggc cgatgtcacc gtttccttgc cgcgctcggt
     3241 ggtggcaatg aagatgttgc tgccggtgtt ggcgtccggc gtgtccttgt cgatgaagaa
     3301 cacgcgctgg ccaccggccg attcgatgaa gcggccgggc gacacgcgct cgatgtcgcc
     3361 ccggccctga tagcggtcgc gcagctcgcg cgcctgctgg ttcgaccacg gccacaccac
     3421 cagcgccagc accgccaccg acagcagcac cggccaggca aagcgcagcg tgggtttcag
     3481 aaaactccac aaaccctggc cactggagaa ccacaccacc atctcgctcg acgcgtacat
     3541 gcgcgtcagc gtggcggtga tggcgatgaa caggctgagc gtgaggatgg tgggcagatg
     3601 ccccagcacc atgaagccca tcaccagcat cacctccgac gggttgacgt tgcctcgcgt
     3661 ggcctgaccc agcgtgcgga tcagcatcac ggtcatgacg atggtgatca gcaccaccag
     3721 ggtggcgccg aagctgcgcc ccagctcttt gcgcagggat gaatcgaata acatcagacg
     3781 acaaggaaaa cgctgattat ggactttcga ctcgactccc tgatcctggc cgaagcggcc
     3841 gccaccaagt cgccggcctt gatggtgctg gtggccgatg gcttcgaacc gggtgcggat
     3901 gcgctgtcgg cgctcatcca gcgcgcgctg ggcagcggcg acttcaagac cgaggccggt
     3961 gcactgctgt ccagctacgg cgcggcgcgc gtgacggccg agcgtgtggt gctggtcggc
     4021 gtgggcgacg ccagcgggcc gcaggtgcgc aaggcggtgt cggccggcgt gctggcgctg
     4081 cgcggcggcc cggcgaaatc gctggtgctg tgctttgggc agccggccga cgcggcggcg
     4141 gtgcgtgcgg ccacgctggc ggtcgccgag tcgtgctacg cctacggggc caccaaggga
     4201 aagaaaaacc agaaggatca gaagaaggcg ccaccgcgcc tgaccgacgt gctgatcggc
     4261 gtgcccgatg ccgagccggt gcgcgatgcc ttccgcatcg gcgtcggcat tgccagcggt
     4321 gtggagttgg cgcgcgaatg ggccaaccgc cccgccaatc atgccacgcc gaccatgctg
     4381 ggcgaggcgg cgcgcgactt ggcggatttg ccgcgcgtcg attgcgaggt gctcgggccc
     4441 aaagaggtcg ccaagctcgg catgggcgcg tttcaggccg tcgcgcaggg gtcgaaggaa
     4501 gaattgcgct tcatcgtgct gcgctaccac ggcgcgccca agtccgaggc accggtggtg
     4561 ctggtcggca aggggatcac gtttgacacc ggcggcatct cgctcaagcc cgcgcctgag
     4621 atggacgaga tgaagtacga catgagcggc gccgccagcg tgctgggcac cttccgcgcc
     4681 ttggctgagc tgaagccggc catcaacgtc gtcggcctga tccccgcctg cgaaaacatg
     4741 ccggacggcg gcgccatcaa gccgggcgac gtggtcacca gcctgagcgg ccagaccatt
     4801 gagatcctga acaccgacgc cgaaggccgc ctggtgctgt gcgacgcgct gacgtacgcc
     4861 gaacgcttca agccgcgagc ggtgatcgac atcgccacgc tcaccggcgc ctgcgtgatc
     4921 gcgctgggcg gcgtgcgcag cggcctgttc agccccgacg acgcgctggc cgacgcgctg
     4981 ctggccgccg gcgaccgcgc gctcgacctg tgctggcgcc tgccgctgga cgccgactac
     5041 gccgaggcgc tgaaaaccca ttttgccgac gtcgccaacg tcggcggccg cccggcaggc
     5101 gcggtgacgg cggcgaaatt cctgcaacgc tttgcgaccg agtacccgtg ggcgcacctc
     5161 gatattgccg gcacggcctg gaaaggcggc gccgccaagg gcgccacggg ccgcccggtg
     5221 gggctgctgg tgcagtacct gctggcacag gcggggcccg atacggccaa gccgagcggc
     5281 aaggccaaga gcgcaaaaaa ggccaagagc accaaggcgc ggcgcgcttg acgctttgca
     5341 cggcgagcgc gcgcatcatt caccatgacg ttcgtttgtg aaagacctcc ctgcggccgc
     5401 gcagcaggcc cccggtggct gcggagcaag ccatgccgga acgccgtggc cggggttccc
     5461 ccggccgcca gcgttgtccc ccttgggagg aaggcgccgc aggcgactca ggggggggca
     5521 tgacccatat tgggttccac ttcaacatgc ccgacaagct ggcctacgcc tgccgcctgc
     5581 tgcgcaaggc ctacgcagcg ggcgggccgg tgggcgtggt ggggccggcg ccggccatcg
     5641 aggcgctcga caccgagctg tggcgctttt ccgcgctcga cttcatcccg cattgcctgg
     5701 ccagcgcgcc ggaggccatg cgcgtgttca cgcccatcgt gctggcccag gattgtgcgg
     5761 acttgcccca gactggggtg ctggtgcacc tgggtgaaca ggtgccagcc ggcttcgagc
     5821 ggttcgagcg cctgatcgag ctggtggaca ccagcgacgc cgaccgctca gcggcgcgcc
     5881 agcgctggcg ccactatgcc gaccgcggct acccgattca gcggcacgac ctggccgcga
     5941 aggaatgatg aacatgtcca ccaccgaaga cgacgcccag ccgcgctccg cgccgcgttt
     6001 tgtgccgaca ctgaccgagg tggtcggcgc ggccgatgca tccgcccccg ccgcagcccc
     6061 ttcggtacca gcaccatcaa cgcccgtcgc acccccgccc atgccgtcga tcggcgcggc
     6121 cacggcagcc cacaccgccg acgccatgct ggcgcgcatc gggccagacc tggaccgcca
     6181 gatcgccgaa gccatcggcc gcgtgctgca cgagcaactg ctgggcttca acggccgggt
     6241 gcagaaggcg gtggccgaag tggtgcgcga ggcggtggcc aaggcgcttg cgcaggatgc
     6301 gcatggccgc gatgctggga caaaccccta gtgtggcgtg cgccgcaccc tatcgttttc
     6361 ctgattagac tggcacgcgg tttgcgttat tgttcgcgcc cttgaataac catttttctt
     6421 tggagagaga cggatgaaca gaaaactgag cttgacggcg atttttgtcg cggcttctct
     6481 ggccgctgcc tgtggcaaga aggaagagcc ggcgcctacc ccggccgcgc ccgcacctgc
     6541 gccggcggca caagcccccg cacccgcgcc cgccgctggc ggcgagatgg tggtgcgcat
     6601 tggccacgtg ggcccggtgt ctggcccgca ggcgcactac ggcaaggaca acgaaaatgg
     6661 ctcgcgcatg gccatcgaag acctgaacac gcagaacatc gtgatcggcg gcaagaaggt
     6721 caagttcgag atccaggctg aagacgacgc ggccgatccc aagcagggca ccgccgcggc
     6781 gcaaaagctg tgcgacgcca aggtcaacgg cgtggtgggc cacctgaact cgggcaccac
     6841 catcccggca tccaaggtct acaacgactg cggcatcccg cacgtcaccg gcgcggccac
     6901 caaccccgac ctgaccaagc ccggctacaa caccacctac cgcatcatcg ccaacgatct
     6961 ggcgctgggc gctggcctgg ccaactacgc ggccgacgac ctcaagctga agaagatcgc
     7021 tgtcatcgac gaccgcacgg cctacggcca gggcgtggcc aaggtgtttg ccgaaaccgc
     7081 caaggccaag ggcatccaga tcgtcgacga gcagttcacc accgacaagg cgaccgactt
     7141 catggccatc ctgacggcca tcaaggccaa gggcccggac ggcgtgttct tcggtggcat
     7201 ggacccgcaa gccggcccca tgctgcgcca gatggaacag ctgggcatga acaacgtcaa
     7261 gtacttcggc ggcgacggca tctgcacgtc tgaaatcgcc aagctggccg ctggcgccaa
     7321 gacgctgggc aacgtgatct gcgccgaagg cggcgcctcg atccagaaga tgccgggcgg
     7381 caccgagtgg aaggcgcgct acgacgccaa gtaccccaac cagttccagg tctacagccc
     7441 gtacacctac gacgccacga tgctgctggc cgacgccatg aagcgcgccg attcggccga
     7501 ccccaaggtc tacatcccca agctgatcga atcgaactac aagggcgtca ccgccaacat
     7561 ccagttcgag cctaacggcg aaatgaagaa cgccgccatc acgctgtaca agtacgtgga
     7621 cggcaagaag acgccgctgt gatgctgcgt tgatcgtctg aacgtcaaag gccaccgcga
     7681 ggtggccttt tttcttgggt tagctgccag ggtgtcgcgt cagccagcgc ccggcgcgca
     7741 gtcggcgcgc caggctgcgt tccaccggca acggttcgcc gtgcaggccg gcggtcagga
     7801 cgtcggcgca cagcagcgcc agcgtcaggc cgcgtgcgcc caggccagtg agcaggtgaa
     7861 ccggcaacgg cggcgtcgat acttctgaat tgatagctgc tggcgcttgt ccatcaagcg
     7921 ctggcgcctg attttgttca tgaaccgacc atgcgccgac ggccggcagc cggtcgggca
     7981 gggtggcgcg cacgccggcc caggcgccgg cgcggccgtc gacccactgc ggcgccagcg
     8041 cgttggcggc gcgcggcagc agttcggtca gacgctgcag gttgtgctga tggtcggccg
     8101 gcagcacgtc ggcgcgacgg ttgccacgct cgaaggtgga gccgatcacc cacagcgggc
     8161 catcggcgcc cggcacatgc gcgatcaggc tgccgtggcc gttgacggga aagcgcggca
     8221 gcgcctcgtc cgccgcgccg cccggcatgg ggccgaaggc gacctggccg cgcagcgcgt
     8281 gcaggggcag ggcgctggcg tcctggtcgc ctgatgcctg cagcagcccg agcgaatcga
     8341 aaccggccgt gatgaccacc agttccgcct cggccagcag cgcgccggcc gtgtcggcca
     8401 gccgccagcg cgcgccatcg gctgtcacgc gcgccacggg cgaggcgccg cgccacgtga
     8461 tgccgtgcgt ttgcagcatc gcacgcacca gcgcggcggg gcgcagccag ccagcgtggg
     8521 tgtgccacag ggcgcgctcg gcggcgttca gcggcacgcc ggcttgcgcg gctttttctt
     8581 gcgtaatttg ggtttcggcg cttgccacgc ctgcgccagc agcttctgaa tcgatagcgg
     8641 cggcccagtc ggccggcagg cgccgttcgc cgggcgcgtg gcgctccagc acgccggtgg
     8701 cgccgaaatc gatgccgtct tgcagcaagt cgcgcgccgt ggcgagcgtg gcccgcgcgc
     8761 cggcacgggt caggcgcgac agcggtcggt cgtcgggcga cacgtgggcc gccaccacgc
     8821 cggcgggcaa gccggatgcg ccggcggcag gatgagcggc actgtcgagc accgtgacct
     8881 gccagccgcg caacgccagc gcgcgcgcca cggcggcgcc ggccaac
//
LOCUS       DAOMVO010000077         8903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2012_length_8903_cov_15.401673, whole genome shotgun sequence.
ACCESSION   DAOMVO010000077 DAOMVO010000000
VERSION     DAOMVO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_8903_cov_15.401673"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="PKV30_05510"
     CDS             <1..177
                     /locus_tag="PKV30_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRL29709.1"
                     /translation="LGHVAEGVYSARTVLQRALHLGVDMPITECVVALLDGDLKPVEA
                     VAALMGRDPKGELL"
     gene            275..967
                     /locus_tag="PKV30_05515"
     CDS             275..967
                     /locus_tag="PKV30_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29710.1"
                     /translation="MRPIRSTRALRLIAAAACAAALAAGCSSMSGGSSHPMSFFITSS
                     NPGQGGNLGGLAGADAQCQRLADAVGAGGKTWRAYLSTVAADGRPAVNARDRIGSGPW
                     VNAKGVTVATSVADLHSANAKLGKANSLTEKGEVVTGFGDSVNRHDILTGSRPDGTVA
                     VPEAGKDTTCGNWTRGEGGSAIVGHHDLAGTNPDPVANRSWNASHGTRGCSVPQLAQS
                     GSAGLLYCFAAK"
     gene            complement(1053..1562)
                     /locus_tag="PKV30_05520"
     CDS             complement(1053..1562)
                     /locus_tag="PKV30_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HRL29711.1"
                     /translation="MGLLDSVLGSVLAGQNQQSGGLGGLGNVLGGMLGGQQAQQQGPG
                     GLNMGMVAALAPILVSMLANNSQQGGLGGLLDQFTRAGAGNAAQSWVGRGANQSVSPD
                     QVTQALGPDVIGQIASQLGVGHGDAAGGIAHILPELIDKLTPHGQAPQGGLGSGDDIV
                     GMLGKMLQR"
     assembly_gap    1596..1695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1783..2640)
                     /gene="panC"
                     /locus_tag="PKV30_05525"
     CDS             complement(1783..2640)
                     /gene="panC"
                     /locus_tag="PKV30_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRL29712.1"
                     /translation="MQIVHSIPELRHALRHAKRPAFVPTMGNLHAGHLALVAQARPLG
                     DATVASIFVNRLQFQPHEDFDSYPRTFEADRAALESAGCDVLFAPREADLYPEPQTFK
                     VVPDPALADLLEGHFRPGFFTGVSTVVMKLFMAVFAGKPQGVAVFGQKDYQQLMVIRG
                     LVRQFALPIEIVGHATERAADGLALSSRNGYLSETERAEALQLSLTLRGLARDALAAA
                     HALPAQLPTLEAAALQQLAARGWQPDYLTVRRRADLLPPQAGDGAGALVALGAARLGA
                     TRLIDNLEL"
     gene            2792..4203
                     /locus_tag="PKV30_05530"
                     /pseudo
     CDS             2792..4203
                     /locus_tag="PKV30_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            complement(4402..5280)
                     /gene="panB"
                     /locus_tag="PKV30_05535"
     CDS             complement(4402..5280)
                     /gene="panB"
                     /locus_tag="PKV30_05535"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRL29713.1"
                     /translation="MSTTPPPASPYGTLPPASPPAQRKPISLPRLAQLHASGEKISML
                     TAYDATFAAVADAAGVECLLVGDSLGMVCQGLPSTVGVSLDAMRYHTESVSRGLHRVQ
                     ATAWLIADLPFGSYHESPEQALRSATMLMQAGAHMVKLEGGGWTAPTVRFLVERGIPV
                     CAHLGLTPQTVHALGGYRVQGRDEAAAQTLRRHALALQDAGAAMLVLEMVPAALSAQL
                     THELPRCATIGIGAGRGTAGQVLVMHDMLGVNLGRNPKFVRNFMQDAGSVRGAIEAYV
                     RAVKDGSFPDDALHGW"
     gene            complement(5414..6271)
                     /locus_tag="PKV30_05540"
     CDS             complement(5414..6271)
                     /locus_tag="PKV30_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRL29714.1"
                     /translation="MLPAPPDTLLLADARAEAPLPEVIDHVAVARLYGEPLFQLPTDL
                     YIPPDALEIFLEAFEGPLDLLLYLIRKQNFNILDIPMAAVTRQYLSYVDEIRSRNLEL
                     AAEYLLMAAMLIEIKSRMLLPPKKTAEGQEPEDPRAELVRRLLEYEQMKLAATRIAEV
                     PQFGRDFLRAQVYVEQALKPRLPDVELADIQQAWADILKRAKLVQHHKISREELSVRE
                     HMSIVLRKLQGRRFAPFEDLFDPAKGVPVLVVTLIALLELAKETLVEITQAEAYAPIY
                     VRLAFTPSG"
     gene            complement(6356..6574)
                     /locus_tag="PKV30_05545"
     CDS             complement(6356..6574)
                     /locus_tag="PKV30_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HRL29715.1"
                     /translation="MSLARIFQRAHYRSEVTQFIDDLKQARPELERKQREGRALLWDK
                     PIDRDLQADLHAGDVAQKPYVYQTAPSL"
     gene            6668..7186
                     /locus_tag="PKV30_05550"
     CDS             6668..7186
                     /locus_tag="PKV30_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRL29716.1"
                     /translation="MKKQITRRIVIGLATALGALALAACDPQAIKELEEGVSTEADVV
                     ARFGQPERVWPEAGGAKTFEYNRQPAGVRNYMITIGPDGRMTALRQVLTPENFRRVQP
                     GMGVEDVRRMLGMPAKQVPYKLQNQIVWTWKFLEPPSETKAFNVVFSPDYRVIRTEIG
                     PDPDGPDMRGGG"
     gene            complement(7259..>8903)
                     /locus_tag="PKV30_05555"
     CDS             complement(7259..>8903)
                     /locus_tag="PKV30_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRL29717.1"
                     /translation="DFAAGLTVAVVALPLAMAFAIASGLKPEAGLFTAIIAGFLISAL
                     GGSRVQIGGPAGAFIVIVYGIVQQYGVGNLIIATALSGVLLFLMGLFKLGTLVRFIPI
                     SVIIGFTNGIAVLIALSQVKDFLGLHIPKMPGDFFGILGALWGHLHTLNPWALGVALV
                     SLAVIIGWQRWLPALGPKVQFSGKLGSVPGSIVALVLATLVVGVFKLPVETIGSKFGG
                     IPAALPAFTLPNFSWESARFLLMPTITLALLGAIESLLCARVADGMIGDRHDPNQELM
                     AQGVANFVTPFFGGMPATGTIARTVTNIKSGASTPVAGMVHALALLVVILVAAPLAGS
                     IPLACLAAILMFVAWNMGEWREFARLKSYRLPYRVTLLAVFFLTVVFDLTVAVDFGIF
                     AACLTFIYRISSLSRSERLTAADHPALVAAGERMQAWRLYGALFFGATKLVEEIEDHL
                     PPHTLVLDLKNVIYVDSTGAEALENLVHTCRQQGVRLIVSGLMNQPLDIARRTGLLTQ
                     LTQKSADDVQPDVAHAIDAALAGLPAAPEGDEQMAYSGA"
BASE COUNT         1487 a         2942 c         2934 g         1440 t
ORIGIN      
        1 ctcggccacg tggccgaggg cgtgtacagc gcccgcaccg tgctgcagcg cgcgctgcat
       61 ctgggcgtcg acatgccgat caccgaatgc gtggtggcgc tgctggatgg cgacctgaaa
      121 cccgtcgaag ccgtggcggc gctgatgggg cgcgatccca agggcgagct gctctgacag
      181 acgccgcgcc ccggcgcggg caggtcggga ttgacgcgcc ccgcggaccc gccgcacact
      241 ggcggcgccc atcaccccat tcaggagaac aaacatgcgc ccgattcgct ccacccgcgc
      301 cttgcgcctg attgcggccg cggcctgcgc cgccgcgttg gcggccggct gctcgtcgat
      361 gtccggcggc agcagccacc cgatgagctt cttcatcacc agcagcaacc cgggccaggg
      421 cggcaacctg ggcgggctgg cgggcgccga cgcgcaatgc cagcgcctgg ccgatgccgt
      481 gggcgccggc ggcaagactt ggcgcgctta cctcagcacg gtggccgccg atggccgccc
      541 ggcggtcaac gcgcgcgacc gcatcggcag cggcccgtgg gtcaacgcca agggcgtcac
      601 cgtcgccacc agcgtggccg acctgcacag cgccaacgcc aagctgggca aggccaattc
      661 gctgactgaa aaaggcgagg tcgtgaccgg ctttggcgac agcgtgaacc gccacgacat
      721 cctgaccggc tcacgccccg acggcaccgt ggccgtgccc gaggccggca aggacaccac
      781 ctgcggcaac tggacgcgcg gcgaaggcgg ctcggccatc gtcggccacc acgacctggc
      841 cggcaccaac cccgacccgg tggccaaccg ctcgtggaac gcctcgcatg gcacgcgcgg
      901 ctgcagcgtg ccgcaactgg cgcaaagcgg cagcgccggg ctgctctact gcttcgccgc
      961 caaataagcg attgcacatg aaaacggcct gcggcgcccg atgggcgcgc gcaggccgct
     1021 ctttttgttg tagcgatcgc tggccgcgcc gatcagcgct gcagcatctt gcccagcatg
     1081 ccgacgatgt cgtcgccgct gccgaggccg ccctgcggcg cctggccgtg gggcgtcagc
     1141 ttgtcgatca gctcgggcag gatgtgggcg atgccgccag ccgcgtcgcc gtggccgacg
     1201 cccagctgtg aggcgatctg gccgatcacg tccgggccca gcgcctgcgt gacctgatcg
     1261 gggctgaccg actggttggc gcctctgcca acccacgact gcgccgcatt gccggcgccg
     1321 gcgcgcgtga actgatccag caagccgccc agcccgccct gctggctgtt gttggccagc
     1381 atcgacacga ggatcggcgc cagcgcggcc accatgccca tgttgagtcc gcccgggccc
     1441 tgctgctgcg cctgttggcc gcccagcatg ccgccaagca cgttgcccag gccgccgaga
     1501 ccgccgcttt gctggttttg acccgccagc accgaaccga gaaccgaatc gagcagaccc
     1561 atggtgaatg tcctttgaat ggaagtaaga ggagannnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnccgcg gaacgggctt ggcccggccg ccagcgttgt ccccctcggg
     1741 gggaaggcgc cgcaggcgac tcagggggtc aatttccggg ggtcaaagtt ccaggttgtc
     1801 gatcaaccgc gtggcgccca gccgcgccgc gcccagcgcc accagcgccc cggcgccgtc
     1861 gccggcctgc ggcggcagca ggtcggcgcg ccgccgcacc gtcaggtagt cgggctgcca
     1921 gccgcgcgct gccaactgct gcagcgcggc cgcttccagc gtcggcaatt gcgcgggcag
     1981 ggcgtgcgcg gcggccagcg cgtcgcgcgc caacccgcgc agcgtcagcg acagctgcag
     2041 cgcctcggcg cgctcggtct cgctcaggta gccgttgcgc gagctgagcg ccagcccgtc
     2101 ggccgcgcgt tcggtggcgt ggccgacgat ctcgatgggc aacgcaaact gccgcaccag
     2161 gccgcgaatc accatcagct gctggtaatc cttctgcccg aacaccgcca cgccctgcgg
     2221 cttgccggca aacacggcca tgaacagctt catcaccacc gtggacacgc cggtgaaaaa
     2281 gccggggcga aagtgcccct ccagcagatc ggccagcgcc gggtcgggca ccaccttgaa
     2341 ggtctgcggc tcggggtaca gatcggcctc gcgcggggcg aacagcacgt cgcagcctgc
     2401 ggattcgagc gcggcgcggt cggcctcgaa ggtgcgcggg tagctgtcga aatcctcgtg
     2461 cggctgaaac tgcagccggt tgacgaagat gctggccacc gtggcgtcgc ccagcggacg
     2521 cgcctgcgcg acgagcgcca ggtgcccggc gtgcaggttg cccatggtgg gcacgaaagc
     2581 cgggcgcttg gcatggcgca gcgcgtgacg cagttcgggg atggagtgga cgatttgcat
     2641 gaaacgagca gttaaaggtg gatatctgta ggctttttcg acagcgcggg ttgtttttga
     2701 actgttgcca aggcgacttt gaaggcgccg ccgtcagtca gcggtgcgac aaattgcacc
     2761 caatcaaggg ttttccctca gaagagttgt catgagtacg tttgagattg tcttgtcagt
     2821 gctgttgttg atgttctttg cctaccgagg ctacacggtg ttgctgctgg cgcccatcat
     2881 ggccgcgctg gccgtgattc tgtcgggtga catggcgcag atgctgccca tctacaccga
     2941 gaccttcatg cgcgcgctgg gcaactacag ctggcgttct ttcccatctt tctgctgggc
     3001 gcgctgtttg gccagctgat ggccgattcg ggcgcggcca ccgccatcgc caagtggatc
     3061 gagcgctcgc tcggcagccg ccatgccatc ctgacggtgg tgctggcctg cggcatattg
     3121 acgtacggcg gcgtgtcgct gttcgtggtg gcgtttgcga tttatcccat cgccaagtcg
     3181 ctgtttcgcg acgctgacat ccccaagcgc ctggtggcgc cggcgattgc gctgggttcg
     3241 ttcaccttca ccatgacggc gctgccgggc acgccatcga tccagaacgc gattccgatc
     3301 aagtatttcg gcaccaacac cttctcggcg ccgggccttg gactgattgg cggtgccatc
     3361 atgttcacgc tgggcatctt gtggctgcgc tcgcgcgaga aggccgcgcg cgccgccggc
     3421 gagggctatg gcgagcacga cgacaacgac gccggcctgc gcgccatggt gggcgaagcc
     3481 gacatgagtg gtgaccccag ccgcaccatg ccgctcatgc tggcgctgct gccgttggtg
     3541 ctggtgatcg gcatcaacgc gctgttcacc tatggcatct tcccgggcat ggactgggcg
     3601 tttctgaaag agcgctttcc caccatggac gccacgcgcc agaccggcat gtgggcgctg
     3661 atcattgcgc tggtgctgtc gatcatcgtg ctgctgctgg tcagcatcgg ccgctgggcc
     3721 aacctgcagc aaagtatcaa caagggcgtg ctgggctcga tgctgccgat tttcaacacc
     3781 gcgtcggaag tgggttacgg cgccgtcatc gccagcctgg ccggctttgc catcatccgc
     3841 gacgcggtgc tgaacgtgtc gagcaacccg ctgatcagcg aggccgtggc catgaacgtg
     3901 ctggccggca tcaccggctc gtcgtcgggc ggtttgtcga ttgcgctgca gacgctgggc
     3961 gccgactact tgcgcatggc caacgaagcc ggcatcagcc ccgatttgct gcaccgcgtg
     4021 gcggtgatgt cggccggcgt gttcgattcg ctgccgcact gcggcgccat catcacgctg
     4081 ctgtcgatct gcaagctgac gcacaagcag tcgtacctga acatcgcggc gatgaccatc
     4141 gtgattccgc tcatcgcgct gagcgtggtg atcgcgctgg gcacgatgtt tgggtcgttc
     4201 tgaatcgttt tgaacgcaaa aggcgcaaag atggcgcaaa agaccgcaaa aaaatcctta
     4261 gaagcttttt gcgccttttg cgaaacctct gcgccctttg cgttcaaaga gaagaagcgg
     4321 tccattgtcg ataagcatga aaaacggcac cagcgcttgt gtggccagcg cgaccagcta
     4381 tcgatagtga agcgccgcgg tctaccagcc gtgcagcgcg tcgtccggaa agctgccgtc
     4441 cttcaccgcg cgcacatagg cctcgatggc gccgcgcacg ctgccggcgt cctgcatgaa
     4501 gttgcgcacg aacttcgggt tcctacccag gttcacgccc agcatgtcgt gcatcaccag
     4561 cacctggccg gcggtgccgc ggccggcgcc aatgccgatg gtggcgcagc gcggcagctc
     4621 gtgcgtgagc tgcgccgaca gggcggcggg caccatctcc agcaccagca tggcggcgcc
     4681 ggcgtcctgc agtgccagcg cgtggcggcg caaggtttgc gcggccgcct catcgcgccc
     4741 ctgcacgcgg tagccgccca gcgcgtgcac ggtctgcggc gtcaggccca ggtgcgcgca
     4801 cacggggatg ccgcgctcga ccagaaagcg caccgtgggc gccgtccagc cgccgccttc
     4861 cagcttgacc atgtgcgcgc cggcctgcat cagcatggtg gctgagcgca gggcttgctc
     4921 gggcgactcg tggtagctgc caaacggcag gtcggcgatc agccaggccg tggcctgcac
     4981 ccggtgcagc ccgcgcgaga cgctctccgt gtgatagcgc atggcgtcca gcgacacgcc
     5041 cacggtggac ggcaacccct ggcacaccat gccgagcgaa tcgcctacca gcaggcattc
     5101 gacccccgcc gcgtcggcca cggcggcaaa ggtggcgtcg taggccgtca gcatggagat
     5161 cttctcgcca ctggcgtgca gttgcgccag gcgcggcagg ctgatgggct tgcgctgcgc
     5221 cggcggcgag gcgggcggca aggtgccgta gggggaggcg gggggtggcg tcgtgctcat
     5281 ggaaagcctt caagaaacag ccggacgcga aggacgcgaa aatttggcga aggacgcaga
     5341 aaattcaaaa aaaactttgg gctgttcttt cgcgtccttc gcgtccggta ttccgggtgt
     5401 tcgaccgggt cgatcagcca gacggcgtaa acgccagccg cacgtaaatc ggcgcatacg
     5461 cctccgcctg cgtgatctcg accagcgttt ccttggccag ctccagcagc gcgatcagcg
     5521 tcaccaccag caccggcacg cccttggcgg ggtcgaacaa gtcctcgaac ggcgcaaagc
     5581 ggcggccttg cagcttgcgc agcacgatgg acatgtgctc tcgcacgctg agttcttcgc
     5641 ggctgatctt gtggtgctgc accagcttgg cgcgtttcag gatgtcggcc caggcctgct
     5701 ggatgtcggc caattccaca tccggcaggc gcggcttcag tgcctgctcg acatacacct
     5761 gcgcgcgcag aaagtcgcgg ccaaattgcg gcacctcggc gatgcgcgtg gcggccagct
     5821 tcatctgctc gtattccagc aggcggcgca ccagttcggc gcgcgggtct tcgggttctt
     5881 gcccctcggc cgtcttcttg ggcggcagca gcatgcgcga tttgatttcg atcagcatcg
     5941 ccgccatcag caggtattcg gccgccaact ccaggtttct gctgcgaatt tcgtcgacgt
     6001 agctcagata ctggcgcgtg accgcggcca tcggaatgtc gaggatgttg aagttctgct
     6061 tgcggatcag gtacagcagc agatccagcg ggccttcaaa cgcttccaga aagatttcca
     6121 gcgcgtcggg cgggatgtac aggtcggtcg gcagctgaaa cagcggctcg ccgtacagcc
     6181 gcgccactgc cacgtggtcg atcacctcgg gcagcggcgc ctcggcgcgg gcgtcggcca
     6241 gcagcagcgt gtcgggcggg gcgggcagca tcagattgct cgcgtattta tagctgctgg
     6301 cgcttgtcca tcaagcgtca gagcctgatt gagtgcttga gcggggtcgc gccgctcaaa
     6361 ggctgggcgc ggtctggtag acgtagggct tttgcgccac gtcgccggcg tgcaggtcgg
     6421 cctgcaggtc gcggtcgatc ggcttgtccc acagcagcgc gcggccttcg cgctgcttgc
     6481 gctccagctc gggccgcgcc tgcttgaggt cgtcaatgaa ctgcgtgacc tcggaacggt
     6541 aatgggcgcg ctggaagatt cgggcgaggg acatacactg agactctttt gtcgaacccg
     6601 tccgacggcg ccaccatggc ccgccgcgtt acaggcattc gatccatttt aacgggggca
     6661 cgcagccatg aagaagcaga tcacgcgccg catcgtcatc ggcctggcca ccgcgctggg
     6721 cgcgctggcc ctggccgcgt gcgatccgca ggccatcaag gagctggaag agggcgtctc
     6781 gaccgaggcc gacgtggtgg cgcgctttgg ccagcccgag cgcgtgtggc ccgaagccgg
     6841 tggcgccaag acctttgaat acaaccgcca gccggccggc gtgcgcaatt acatgatcac
     6901 catcggcccc gacggccgca tgacggcgct gcgccaggtg ctgacgcccg agaacttccg
     6961 ccgcgtgcag cccggcatgg gcgtggagga cgtgcgccgc atgctgggca tgccggccaa
     7021 gcaggtgccg tacaagctgc agaaccagat cgtgtggacc tggaagttcc tcgagccgcc
     7081 gagcgagacc aaggccttca acgtcgtctt cagcccggat taccgcgtga tccgcaccga
     7141 gatcgggccc gatccggatg ggccggacat gcgcggcggc ggctgattct ttgagtgaaa
     7201 atggccgcca gcgcttgtct gacaagcgcg agcagctatt gaatttgtag ttttccgttc
     7261 aagcgcccga atacgccatc tgctcgtcgc cctccggcgc tgccggcaaa ccggccagcg
     7321 ccgcgtcaat ggcgtgcgcc acgtcgggct gcacgtcgtc cgcgcttttt tgcgtcaact
     7381 gggtcagcaa gccggtgcgc cgcgcgatat cgagcggctg gttcatcagg ccgctgacga
     7441 tcagccgcac gccctgctgg cggcaggtgt gcaccaggtt ttccagcgcc tcggcgccgg
     7501 ttgaatcgac gtagatcacg ttcttcaaat cgagcaccag cgtgtgcggc ggcaggtggt
     7561 cttcgatttc ctcgaccagc ttggtcgcgc caaagaacag cgcgccgtac aaccgccagg
     7621 cctgcatgcg ctcgcccgcg gccaccagcg ccgggtggtc ggcagccgtc aggcgctccg
     7681 agcgcgacag gctggagatg cggtagatga aggtgaggca ggcggcgaag atgccgaaat
     7741 caaccgccac cgtcaaatcg aacaccacgg tgagaaagaa caccgccagc agcgtgacgc
     7801 ggtagggcag gcggtagctt ttcaggcgcg caaactcgcg ccactcgccc atgttccagg
     7861 ccacgaacat caagatggcg gccaggcagg ccagcggaat cgagccggcc agcggcgccg
     7921 ccaccagaat caccaccagc agcgccagcg cgtgcaccat gccagccacc ggcgtgctgg
     7981 cgccgctctt gatgtttgtg acggtgcgcg cgatggtgcc ggtggccggc atgccgccga
     8041 aaaagggcgt gacgaagttg gccacgccct gcgccatcag ctcttgattg gggtcgtggc
     8101 ggtcgccgat catgccgtcg gccacgcgcg cgcagagcag tgattcgatg gcacccaaaa
     8161 gcgcgagcgt aatggtcggc atgagcagaa aacgcgccga ttcccagctg aaattgggca
     8221 gcgtgaaggc cggcagcgcc gctggaatgc cgccgaactt gctgccgatg gtttccaccg
     8281 gcagcttgaa cacgcccacc accagcgtgg ccagcaccag cgccacgatg gagcccggca
     8341 cgctgcccag cttgcccgag aactgcacct tgggccccag cgccggcagc cagcgctgcc
     8401 agccgatgat gacggccagc gacaccagcg ccacgcccag cgcccacggg ttgagcgtgt
     8461 gcaggtgccc ccacagcgcg cccaggatgc cgaagaaatc acccggcatc ttggggatgt
     8521 gcaggcccag aaaatccttc acctgcgaca gcgcaatcag caccgcgatg ccgttggtga
     8581 aaccgatgat caccgagatg ggaatgaagc gcaccagcgt gcccagcttg aacagcccca
     8641 tgaggaacag cagcacgccc gacagcgccg tggcgatgat gagattgccc acgccgtact
     8701 gctgcacgat gccgtagacg atgactatga aggcgccggc cggcccgccg atctgcacgc
     8761 ggctgccgcc cagcgccgag atcagaaagc ccgcgatgat ggcggtgaac aggcctgcct
     8821 cgggcttgag ccccgacgcg atcgcaaacg ccatcgccag cggcagcgcc accacggcca
     8881 ccgtcaggcc ggcggcaaag tcc
//
LOCUS       DAOMVO010000078         8832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2030_length_8832_cov_16.191409, whole genome shotgun sequence.
ACCESSION   DAOMVO010000078 DAOMVO010000000
VERSION     DAOMVO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_8832_cov_16.191409"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..995
                     /locus_tag="PKV30_05560"
     CDS             405..995
                     /locus_tag="PKV30_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HRL29718.1"
                     /translation="MPTTHLLYLHGFRSSPQSTKAQMMAARVQADHKKVRWWCPQLPP
                     SPREAMALIQRGTADWPADQMAVVGSSLGGFYATALAQQRGCRAVVLNPAVDPARDLA
                     AHIGEQTAWHDPAEHFFFRPEYIDELRALQTGPLTHPERTFAVIAKGDELLDWREMTA
                     RYASARIRLVEGSDHALSDFDAHIDAVQEFLALERL"
     gene            1128..3203
                     /locus_tag="PKV30_05565"
     CDS             1128..3203
                     /locus_tag="PKV30_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRL29719.1"
                     /translation="MHLLFDEAGKFLAGRILSDAEASAQVELDSGKRVKVKAANQLLR
                     FEKPLPAELMASAQALAADIELPLAWEFAPEDEFGFAELATDYFGGTPDVAHQAAMLL
                     ALFEAPHYFRRAGKGRFKKAPADIVAQALAAIEKKKQIAAQIDAWAGELAAGTTPAPI
                     REQLYKILFRPDKNAPEYKAVVEASRATHLAPLALLQKAGAIESAYQFHWQRFLFDNF
                     PKGTGFGALAAPAITEELPLAEVQAFSIDDSSTTEIDDALSLQGLGTGTVVLGIHIAA
                     PGLAIQPGDALDETSRARLSTVYMPGYKITMLPQDVVDAYTLSEGRAVPAVSLYVTLD
                     EATLEVKASETKLERVPIAANLRHDQLDALVTEAWLSGADVSNENTAQRLPIQREQLS
                     FLYRLALHLKAQRERVRGKPETFNRPDYNFKLVDHDGAVPTGDEQVAITTRQRGAPLD
                     LIVAEAMILANSTWGEWLKSLSVPAIYRSQASLLPGIKVRMGAKALPHAGIGVPSYAW
                     STSPLRRYVDLVNQWQIIAAARHGKTAALAAPFKPKDAQLFAIISAFDAAYSAYNAHQ
                     GSMERFWTLHYLRQQGITELTASLIKEQAGGAWLARADTLPLVFTVLGAQGLPRGTQV
                     RVKLGAIDDIALDVGGTVLEVLDADAPADTAPDDEDETSAAGPIAIAVDVNEAEGEKE
                     GSRADNPAP"
     gene            3203..4099
                     /locus_tag="PKV30_05570"
     CDS             3203..4099
                     /locus_tag="PKV30_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRL29720.1"
                     /translation="MLRFFKSLSTLQWALGLSVLLHAALLTVRFVDPEGFKRVFEDTT
                     LDVILVNAESDEKPVKAQALAQATLAGGGEAEGKRIVSTPLPPAPVEAQGDSAVNENQ
                     RRIDNMMEQQEQLLAQVRQQLATIPKVDPQKLADDPEARAQEERRQQLSKLLAAIERR
                     VEEENSRPRKRYLSPATLGTTYAVYYDEMRRKIEAEGTENFPHVAGRKLYGELLMALL
                     INHDGRILDARVVHSSGDRNLDRLAALIASRAAPFGNFTSAMRKDTDQFDVTARFKFT
                     RDMTLETTLQADQLSSTLQQPR"
     gene            4099..4824
                     /gene="mtgA"
                     /locus_tag="PKV30_05575"
     CDS             4099..4824
                     /gene="mtgA"
                     /locus_tag="PKV30_05575"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRL29721.1"
                     /translation="MRALGRWLLLVVVAALALQLFFIGRIAWMALVDPQSTAFQRSEA
                     WRLATSGQLRWRQDWRDYEQISDHLKRAVIASEDDDFVNHEGVDWEAIDKAWERNAKA
                     EEAAAQRAAQNRPVRPVKIRGGSTITQQLAKNLLLSGERTLLRKGQELVLTFALEHLL
                     SKRRILEIYLNNVEWGEGVFGAEAASQHYFRKSAAQLSAWEAARLAVMLPRPRYFEQQ
                     PRSVYLNSRTRTIVARMRNAELP"
     gene            4821..5465
                     /locus_tag="PKV30_05580"
     CDS             4821..5465
                     /locus_tag="PKV30_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL29722.1"
                     /translation="MTDLQHTQRHPWLAKVFGYALLGFVRLLTGAQARWYGSPPKAEQ
                     RIYFANHQSHADLVMIWAALPEELRSITRPIAARDYWTKSDFKRWITTAVFNAVYVDR
                     ERQGDEDPLQPLIDALQSGDSLIIFPEGTRGHAAEPQAFKAGLYNLALRFPDVVLVPA
                     WIANVQRVMPKGEVVPVPVLCSVTFGAPIRLEPGEERRAFLDRARAAVIALRDV"
     gene            5468..6451
                     /locus_tag="PKV30_05585"
     CDS             5468..6451
                     /locus_tag="PKV30_05585"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL29723.1"
                     /translation="MISFIRNLSADQQVTALFLLVFGVLTLATGVLVALSLRERGDGS
                     PDGTDHLAHARVLLRHSWFMTLVFWFAWALGETAATVLFAFVSFFALREFMTLSPTRR
                     GDHRALVLAFFVVLPVQYWLVGARYFNLFTVFIPVYVFLLLPVLSALGNDPQGFLERS
                     AKMQWGIMVCVYGLSHVPALMLLHFGGWQGRNAFLVFFLAMVVQTCMLVQHLVNRHRP
                     GQPVAPHVSASFAWTSWLAGLAAGGALGVLLSGITPFAPGQALAMALIACVAGSLGHL
                     VMKALKRDRGVTNWGPRTASVTGSAGLLDRVDALCFAAPVFFHSVRWYFGL"
     gene            6492..7028
                     /gene="ruvC"
                     /locus_tag="PKV30_05590"
     CDS             6492..7028
                     /gene="ruvC"
                     /locus_tag="PKV30_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRL29724.1"
                     /translation="MRILGIDPGLRTTGFGVLDVEGAALRYVASGVIQTSSEAQGDLP
                     ARLKVLFDGIGELIARYQPDAAAVEIVFVNVNPQATLLLGQARGACVTALVATNLPVA
                     EYTALQMKQAVAGHGKAAKAQVQAMVQRLLQLPGAPRPDAADALGLAITHAHAGRAVA
                     LLHAAGAGRTTSSMYRRR"
     gene            complement(7128..8051)
                     /gene="lpxC"
                     /locus_tag="PKV30_05595"
     CDS             complement(7128..8051)
                     /gene="lpxC"
                     /locus_tag="PKV30_05595"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRL29725.1"
                     /translation="MLAQRTLKSLTRAVGVGLHSGERVELTLRPAPPDTGIVFRRIDL
                     PQPVDIPVSALSVTDTRLASTLSAGDAKVRTVEHLMSACAGLGLDNLYIDITAEEVPI
                     LDGSASSFVYLLQSAGIELQKAPKKFLRVMQPVEVREGEGDQLKWARLDPHHGFTLSF
                     EIDFQHPVVDATVQRVEFDMGSGRYSRDIARARTFAFSKDLDMMRANGLALGGGLDNA
                     IVMDDTKVLNADGLRYAEEFAKHKMLDAIGDLYCVGKPMLAAYSAFRSGHALNNKLLR
                     ALLAQQGASEIVSFEDEKKAPTGFAQLSRAW"
     gene            complement(8261..>8832)
                     /locus_tag="PKV30_05600"
     CDS             complement(8261..>8832)
                     /locus_tag="PKV30_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRL29726.1"
                     /translation="EPGKAMMGTATASGPDRARIAAEQAVACPLLEGIDLSGAKGVLV
                     LVTASKGTLKLNESKEAMNTIRAYASPDAHVIFGAAYDDALGDEIRVTVVATGLSRQG
                     VRRQPITVVQGGLRTGTDNVPFHVPTLNQSVGGGMGHAAPQQPDYGNMAVPSVWRTNR
                     TQAAAKVDALSSGGMDDFEIPAFLRKQAD"
BASE COUNT         1446 a         3008 c         2937 g         1441 t
ORIGIN      
        1 cagcccggag cggtggcgcg ctgcgcgcgg ccgagcgcag cgatggcccg tgcgttgact
       61 tcaaccccct tctggccgtg ccgagaagcg cagggcgtgg ggcgggcggg cagcgcagca
      121 tgcccgcatc gtgctctgac tcgctgccgc tgtttgaacg gagcgctgta agcgcgaagt
      181 gagttcggca gcgccgcccc gcgcacgagc atcgcaggct gcccgcagct aagcgcaggg
      241 acacggccag tggggccgcc tttctttgcc tactttcttt ggccgagcaa agaaagtagg
      301 tcgcccgccg gggcgaactc ccggccagcc gcgcatccaa cgcccccgcc cctcacccca
      361 ccctctcccc aaagggggcg atggagagca cctaccatcc ccgcatgccc accacccacc
      421 tcctctacct ccacggcttc cgctcctccc cccaatcgac gaaggcccaa atgatggccg
      481 cccgcgtgca ggccgatcac aaaaaggtgc gctggtggtg cccccaactc cccccctccc
      541 cgcgcgaagc catggccctc atccagcgcg gcaccgccga ctggccagcg gatcagatgg
      601 ccgtcgtcgg ctcctcgctc ggcggcttct acgccaccgc cctcgcccag cagcgcggct
      661 gccgcgcggt ggtgctcaac cccgccgtcg accccgcgcg cgacctggcc gcgcacatcg
      721 gcgagcagac cgcttggcac gaccccgccg agcacttctt cttccgcccc gaatacatcg
      781 acgagctgcg cgcgctgcaa accggcccgc tgacgcaccc cgagcgcacc ttcgccgtca
      841 tcgccaaagg cgacgaactg ctggattggc gcgagatgac cgcccgctac gccagcgccc
      901 gcatccgcct ggtcgaaggc agcgaccacg cgctgtcaga cttcgacgcc cacatcgacg
      961 ccgtgcagga atttctggcg ctggaacgtc tttagtatta ttttgatagc tgctcgcgct
     1021 tgactgtcgt gcgctatcgg cactttttat tcaaaaaatc ttgtccgact gagcccgcag
     1081 ccacacgccg cccccagccc cggcgggatg ggacaatcgc cccctctatg catcttctat
     1141 tcgacgaggc cggcaaattt ctcgccggcc gcattctttc cgacgccgag gcgtcggccc
     1201 aggttgagct ggattcgggc aagcgcgtca aggtcaaggc ggccaaccag ctgctgcgct
     1261 ttgaaaaacc cctgcctgcc gagctgatgg cgagcgccca ggcgctggcg gccgacatcg
     1321 agctgccgct ggcctgggaa ttcgcccccg aggacgaatt cggctttgcc gagctggcca
     1381 ccgactattt cggcggcacg cccgacgtcg cccaccaggc ggccatgctg ctggcgctgt
     1441 tcgaggcgcc gcactacttt cgccgcgccg gcaagggccg cttcaagaaa gcgccggccg
     1501 acatcgtggc gcaggcgctg gccgccatcg agaagaaaaa gcagattgcc gcgcaaatcg
     1561 acgcctgggc cggcgagctg gcagcaggca ccacgccggc gccgatccgt gagcagcttt
     1621 acaagatcct gttccggccc gacaagaacg cgcccgaata caaggccgtg gtcgaagcca
     1681 gccgcgccac gcacctggcg ccgctggcgc tgctgcaaaa ggcgggcgcc attgaatcgg
     1741 cctaccagtt tcactggcag cgctttctgt tcgacaactt tccgaagggc acgggctttg
     1801 gggcgctggc cgcacccgcc atcaccgaag agctgccgct ggccgaggtg caggcgtttt
     1861 ccatcgacga ttcatcgacg accgagatcg acgatgcgct gtcgctgcaa ggcttgggca
     1921 cgggcacggt ggtgctgggc atccacatcg cggcgcccgg cctcgccatt cagcccggcg
     1981 acgcgctgga cgagaccagc cgtgcgcgcc tgtccaccgt ctacatgccg ggctacaaga
     2041 tcaccatgct gccgcaggac gtggtcgatg cctacacgct gagcgaaggc cgggcggtgc
     2101 cggcggtgtc gctctatgtc acgctcgatg aagccacgct ggaggtcaag gccagcgaaa
     2161 caaagctgga gcgcgtgccg attgccgcca acctgcggca cgaccagctg gatgccctcg
     2221 tcaccgaagc ttggctgtcg ggcgccgacg tttcaaatga aaacacggcc cagcgcttgc
     2281 ccatccagcg cgaacagctc tcatttttat accgcttggc gttgcatttg aaagcgcagc
     2341 gcgagcgtgt gcgcggcaag ccggagacct tcaaccggcc cgactacaac ttcaagctgg
     2401 tcgatcatga cggcgcggtg ccgaccggcg acgagcaggt ggccatcacc acgcgccagc
     2461 gcggcgcgcc gctcgatttg atcgtggccg aagccatgat cctggccaac agcacctggg
     2521 gcgaatggct caaaagctta agtgtgccgg ccatctaccg cagccaggcc agtctgctac
     2581 cgggcatcaa ggtgcgcatg ggcgccaagg cgctgccgca cgcgggcatt ggcgtgccga
     2641 gctatgcgtg gagcacatcg ccgctgcgcc gctacgtcga cttggtcaac cagtggcaaa
     2701 tcattgcagc cgcgcgccat ggcaagacgg ccgcgctggc cgcgcccttc aagccgaagg
     2761 acgcgcaact gttcgccatc atcagcgcct tcgacgccgc ctacagcgcc tacaacgcgc
     2821 accagggcag catggaacgc ttctggacgc tgcattacct gcgccagcaa ggcatcaccg
     2881 agctgacggc cagcctcatc aaggaacagg ccggcggcgc ctggctggcg cgcgccgaca
     2941 cgctgccgct ggtgttcacc gtgctgggcg cacaaggcct gccgcgcggc acgcaggtgc
     3001 gcgtgaagct gggcgcgatt gacgacatcg cgctcgatgt cggcggcacc gtgctggagg
     3061 tgctggacgc cgatgcgccg gctgataccg cgccagatga cgaagatgag acttctgccg
     3121 caggccccat cgccatcgcg gtcgatgtga acgaagccga gggcgagaaa gagggcagca
     3181 gggccgataa tcccgccccg taatgctgcg ttttttcaag tccttatcga cgctgcagtg
     3241 ggcgctcggg ctgtcggtgc tgctgcacgc agcgctgctc acggtgcgct ttgtcgaccc
     3301 cgaaggcttc aagcgcgtgt tcgaggacac cacgctcgac gtcatcctgg tcaacgccga
     3361 atcggacgaa aagccggtca aggcgcaggc gctggcccag gccacgctgg ccggcggcgg
     3421 cgaggccgag ggcaagcgca tcgtcagcac gccgctgccg ccggcgccgg tcgaagcgca
     3481 gggcgactcg gccgtcaacg agaaccagcg ccgcatcgac aacatgatgg agcagcagga
     3541 gcaactgctg gcccaggtgc gccagcaact ggcgaccatc cccaaggtcg atccgcaaaa
     3601 gctggccgac gaccccgagg cgcgcgcgca ggaagagcgg cgccagcagt tgtcgaagct
     3661 gctggccgcc atcgagcgcc gggtggaaga agaaaactcg cgcccgcgca agcgctacct
     3721 gagtcccgcc acgctgggca ccacctacgc cgtgtactac gacgagatgc gccgcaagat
     3781 cgaggccgag ggcaccgaga atttcccgca tgtggccggg cgcaagctgt acggcgagct
     3841 gctgatggcg ctgctcatca accacgacgg ccgcattctg gatgcgcgcg tggtgcacag
     3901 ctcgggcgac cgcaacctgg accggctggc ggcgctgatc gcctcgcgcg cggcgccgtt
     3961 cggcaatttc acctccgcca tgcgcaagga caccgatcag ttcgacgtca ccgcgcgctt
     4021 caagttcacg cgtgacatga cgctggaaac cacgctgcag gccgaccagc tgagcagtac
     4081 cctgcagcag ccccggtgat gcgcgcgctg ggtcggtggc tgttgctggt ggtggtggcc
     4141 gcgctggcgc tgcagttgtt cttcatcggc cgcattgcgt ggatggcgct ggtcgatccg
     4201 caatccaccg ccttccagcg ctcggaagcc tggcgcctgg cgaccagcgg gcagctgcgc
     4261 tggcggcagg actggcgcga ctacgaacag atttccgacc acctgaaacg cgccgtgatc
     4321 gcctcggaag acgacgactt cgtcaaccac gaaggcgtcg attgggaagc catcgacaag
     4381 gcctgggagc gcaacgccaa ggccgaggaa gccgccgccc agcgcgcggc gcagaaccgc
     4441 ccggtgcggc cggtgaagat tcgcggcggc tcgaccatca cgcagcaact ggccaagaac
     4501 ctgctgctgt cgggcgagcg cacgctgctg cgcaagggcc aggagctggt gctgaccttt
     4561 gcgctggaac acctgctctc caaacgccgc atcctcgaga tctacctgaa caacgtcgaa
     4621 tggggcgaag gcgtgtttgg cgccgaggcg gcgtcgcagc actatttccg caagagcgcg
     4681 gcgcaactca gcgcgtggga agccgcgcgc ctggccgtca tgctgccgcg cccgcgctac
     4741 ttcgagcagc agccacgctc ggtctatctg aacagccgca cgcgcaccat cgtggcgcgc
     4801 atgcgcaatg ctgagttgcc atgactgacc tgcagcacac acagcgccac ccttggctcg
     4861 ccaaggtgtt cggctacgcg ctgctgggct ttgtgcgcct gctcaccggc gcgcaggcgc
     4921 gctggtacgg cagcccgccc aaggcggagc agcgcatcta ctttgccaac caccagagcc
     4981 acgccgacct ggtgatgatc tgggccgcgc tgcccgaaga gttgcgcagc atcacgcgcc
     5041 ccatcgccgc gcgcgactac tggaccaaga gcgatttcaa gcgctggatc acgaccgccg
     5101 tgttcaacgc ggtctatgtc gaccgcgaac gccagggcga cgaagacccg ctgcagccgc
     5161 tgatcgacgc gctgcaaagc ggtgattcgc tcatcatctt tcctgaaggc acccgcggcc
     5221 acgcggccga gccgcaggcc ttcaaggccg ggctgtacaa cctggcactg cgctttcccg
     5281 acgtggtgct ggtgccggcc tggatcgcca acgtgcagcg cgtcatgccc aagggcgagg
     5341 tcgtgccggt gccggtgctg tgttcggtga cgtttggcgc gcccattcgc ctggagccgg
     5401 gcgaggagcg ccgcgccttt ctcgaccgcg cgcgtgcagc cgtcattgcg ctgcgggacg
     5461 tttgaacgtg atcagcttca tccgcaacct atcggccgac cagcaagtca ccgcgctgtt
     5521 cctgctggtg tttggcgtgc tgacgctggc caccggcgtg ctggtggcgc tgtcgctgcg
     5581 cgagcgcggc gacgggtcgc cggatggcac cgatcacctg gcgcacgcgc gcgtgctgct
     5641 gcgccattcg tggttcatga cgctggtgtt ctggttcgcc tgggcgctgg gcgaaacggc
     5701 ggccacggtg ctgttcgcct tcgtgtcctt ctttgcgctg cgcgagttca tgacgctgtc
     5761 gcccacgcgg cgcggcgatc accgcgcgct ggtgctggcc ttcttcgtcg tgctgccggt
     5821 tcagtattgg ctggtgggcg cgcgctactt caacctgttc accgtgttca tcccggtgta
     5881 cgtgtttctg ctgctgccgg tgttgagcgc gctgggcaac gatccgcagg gttttctgga
     5941 gcgcagcgcc aagatgcagt ggggcatcat ggtgtgcgtg tacgggctgt cgcacgtgcc
     6001 ggcgctgatg ctgctgcact ttggcggctg gcaggggcgc aacgcattcc tggtgttctt
     6061 tctggccatg gtggtgcaga cctgcatgct ggtgcagcac ctggtcaacc ggcaccgccc
     6121 gggccagccg gtggcgccgc acgtcagcgc cagcttcgcc tggacgagct ggctggccgg
     6181 cctggcggcg ggtggcgctc tgggcgtgct gctgtcgggc atcacgccgt tcgcgcccgg
     6241 gcaggcgctg gccatggcct tgatcgcctg cgtggccgga tcgctgggcc atctggtgat
     6301 gaaggcgctc aagcgcgacc gcggcgtgac caactggggc ccacgcacgg cgtcggtcac
     6361 cggcagcgcc gggctgctcg accgggtcga cgcactgtgc tttgcggcgc cggtgttttt
     6421 tcactcggtt cgatggtatt ttgggctcta gcgcaggcct gacaagcgca agcagctata
     6481 aaatcagaag catgcgaatc ctcggcatcg accccggcct gcgcaccacg ggctttggcg
     6541 tgcttgacgt cgaaggggcc gcgctgcgct acgtggccag cggcgtgatc cagaccagca
     6601 gcgaggcgca gggcgatctg ccggcgcgcc tgaaggtgct gttcgacggc atcggcgagc
     6661 tgattgcgcg ctaccagccc gacgcggcgg cggtcgaaat cgtgttcgtc aacgtcaacc
     6721 cgcaggccac gctgctgctg ggtcaagcgc gcggcgcctg cgtgacggcg ctggtcgcca
     6781 ccaatttgcc ggtggccgaa tacaccgcgc tgcagatgaa gcaggccgtg gccggccacg
     6841 gcaaggcggc caaggcgcag gtgcaggcga tggtgcagcg cctgttgcag ctgccgggcg
     6901 cgccgcggcc tgatgcggcc gatgcgctcg gtctggcgat cacccacgcg cacgccgggc
     6961 gcgcggtggc actgctgcac gctgcgggtg cggggcgcac gaccagcagc atgtatcgac
     7021 ggcggtgaac gccggagtct tgatgaaatc gggcgctggc gcttgtccat caagcgcggg
     7081 cagctactat ttttgcagca ttcgcatcac gccctgcgcg tgcgttgcta ccaggcgcgc
     7141 gagagttgcg cgaagccggt gggtgccttt ttctcgtctt cgaagctgac gatttcgctc
     7201 gcgccctgtt gcgccagcag cgcgcgcagc agcttgttgt tgagcgcgtg acccgagcgg
     7261 aaagcgctgt acgccgccag catgggtttg ccgacgcagt acaaatcgcc aatggcgtcc
     7321 agcatcttgt gcttggcaaa ctcctcggcg tagcgcaggc cgtcggcgtt gagcaccttg
     7381 gtgtcgtcca tgacgatggc gttgtcgagc ccgccgccga gcgccaggcc gttggcgcgc
     7441 atcatgtcca ggtccttgct gaaggcgaag gtgcgcgcgc gcgcgatgtc gcggctgtag
     7501 cggcccgaac ccatgtcgaa ctcgacgcgc tgcaccgtgg catcgaccac cgggtgctga
     7561 aagtcgatct cgaagctgag cgtgaagccg tggtgcgggt cgagccgcgc ccacttgagc
     7621 tgatcgccct cgccctcgcg cacctcgacc ggctgcatga cgcgcagaaa cttcttgggc
     7681 gccttttgca actcgatgcc ggcgctttgc agcaggtaga cgaaggacga ggccgagccg
     7741 tcgaggatcg gcacctcttc ggccgtgatg tcgatgtaga ggttgtccag ccccaggccg
     7801 gcgcaggccg acatcaggtg ctcaacggtg cgcaccttgg cgtcgcccgc agagagcgtc
     7861 gacgccagcc gggtatcggt gaccgacagc gcgctcaccg gaatgtccac cggctgcggc
     7921 aaatcgatgc ggcgaaacac gatgccggta tccggcggcg cggggcgtag cgtcagctcg
     7981 acccgctcgc cgctgtgcag accgacgccg acggcgcggg tgagggactt gagggtgcgt
     8041 tgcgcaagca tggccatatt ctaaattccc tcgtgtccgc cagcttgaag atcaaatcag
     8101 gctgcagggc tttcggagca agcgcgagca gctatcgatt caatagcaaa aagacgatcc
     8161 tctggcaaac gccctggcca gggcacccgc ggaaggcttt gccgcccgct gggtgcgcca
     8221 ccttgcgggg gaggcgcgca gcgcctcggg ggtggtccat tcaatcggct tgcttgcgca
     8281 ggaaagccgg gatctcgaag tcgtccatcc cacccgacga caaagcatcg accttggccg
     8341 cagcctgcgt gcggttggtg cgccacacgc tgggcacggc catgttgccg tagtcgggct
     8401 gctgtggcgc cgcgtggccc atgccgccgc ccaccgactg gttcagcgtc ggcacgtgga
     8461 agggcacgtt gtcggtaccg gtgcgcaggc cgccctgcac cacggtgatc ggctggcggc
     8521 gcacgccctg gcgcgacagg ccggtggcca ccacggtgac gcgaatctcg tcgcccagcg
     8581 cgtcgtcgta ggcggcgccg aaaatgacgt gcgcgtcggg cgaagcgtag gcgcggatgg
     8641 tgttcatcgc ttccttcgac tcgttcagct tgagcgtgcc cttggacgcc gtgaccagca
     8701 ccagcacgcc cttggcgccc gacaggtcga tgccttccag cagcgggcag gccacggcct
     8761 gctcggcggc gatgcgcgcg cggtcggggc cgctggcggt ggccgtgccc atcatggcct
     8821 tgccgggctc gc
//
LOCUS       DAOMVO010000079         8736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2069_length_8736_cov_16.300657, whole genome shotgun sequence.
ACCESSION   DAOMVO010000079 DAOMVO010000000
VERSION     DAOMVO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2069_length_8736_cov_16.300657"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PKV30_05605"
     CDS             <1..961
                     /locus_tag="PKV30_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL29727.1"
                     /translation="RHRLVASTDWDEVQHWCRQIYLPYDAKPAGPARQPNAVLDAMQI
                     GQLTLSRFSYGVPVHLSGFDEGAGIGLVLTTLQGAVRHWSSGRTHADTGVGDSYLVDL
                     SRAHYRLDADARHLQVNLTFRHDAMAALHQRWFGCPADERMWARAYRFGGAQSSWMQL
                     LGYVCRCITEMPDAVQHGPLGRHLEEMIGVHLLTQWRQQLDAPTSPTLHSLAPRHVLM
                     AEQHIRDRAREAPTLSALASHAGVSVRTLNAAFREYRQCTPMQALRDARLAGVRADLL
                     AARPGARVRDVAEAWGYAQLGIFAANYRRRFGESPSETLNRRA"
     gene            complement(1156..2682)
                     /locus_tag="PKV30_05610"
     CDS             complement(1156..2682)
                     /locus_tag="PKV30_05610"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL29728.1"
                     /translation="MDMFELKRFGLEAAYPYKQQYDNYIGGQWVPPSAGRYFENVSPI
                     TGKPFTKVAQSDAEDVNRALDAAHAAQAAWAKTSVAERSNILLKIADRMEQHLQTLAI
                     AETIDNGKPLRETMAADIPLAIDHFRYFAGAIRAQEGGISEIDETTVAYHFHEPIGVV
                     GQIIPWNFPLLMASWKLPPALAAGCCVVMKPAEQTPASIMVLMELIGDLLPPGVVNIV
                     NGFGKEAGEALATSTRIAKIAFTGSTPVGQRILRAAADNLIPSTVELGGKSPNIFFAD
                     VMDHDDAFLDKALEGLSMFALNQGEICTCPSRILIQESIYDRFIERALKRVSAIKQGH
                     PLDTSTMVGAQVSQQQMERILGYIDIGRNEGVQTLTGGERNRQGGDINDGFYVKPTLL
                     FGKNDMRVFQEEIFGPVASVTTFKDFDEAIHIANDTAFGLGAGVWSRNGNVAYKAGRA
                     IQAGRVWTNCYHLYPAHAAFGGYKKSGIGRENHQMALNNYQQTKNLLVSYSENKLGFF
                     "
     gene            3032..4981
                     /locus_tag="PKV30_05615"
     CDS             3032..4981
                     /locus_tag="PKV30_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRL29729.1"
                     /translation="MGIATDSLQLARRHLLDHGACLPSALNDRLARSWQRSLHAGLPP
                     FGSRELAEPAGGLSLSQSLEQNAQMLSFARPVMDFMYGQIRGGDCMVVLADVDSMLLH
                     TVGDHGFLTKAQRVELRTGASWRESVRGTNAIGTAIEDASALQVRAGEHYLERNAFLH
                     CSAAPIFTAQGAVAGAIDISGDERASHMHALGLASSAARLIENNWLKARYRDAVRVHL
                     HSAAEGLDTPAEGIVVLSESGTLLGANQEAIRALRLAPHDFFALQLETRLQERLAHLL
                     ARTGREPHTVRLPDGRRMFLRVEQPAQRTPARTVVPEARVDALAASDTGDAQWRSAAQ
                     QVRRILDKPIALLIQGESGVGKEVFARACHDSGPRRGRPFVAVNCAAIPESLIEAELF
                     GYAPGAYTGAQKGGSLGRIREAEGGTLFLDEIGDMPLLLQTRLLRVLQEKKVTPLGGT
                     PVPVDFALVSATHCVLADAVAEGRFRSDLYYRLNGFSVKLPALREREDFDALATRMLA
                     QWDDSGVRIAPDLLAALRGYTWPGNLRQLASVLQTACALRDPAHEPAIDWPHLASDLR
                     DALASPAARVQPAGKAAETRAISVGQADGAPEAAPPAALRKIARQAIAQALADGGGNV
                     SEAARRLGISRQTIYRHLKKADAEA"
     gene            complement(5092..5949)
                     /locus_tag="PKV30_05620"
     CDS             complement(5092..5949)
                     /locus_tag="PKV30_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL29730.1"
                     /translation="MLGAALPAHAQSADTLSKISSSGKMTFAYRESSVPFSYLEGPEK
                     PIGFAVDISNAIADAVRKQTGKSNLTVGWQAVTSANRIPLLQNGTIDLECGSTTNNSA
                     RGKDVDFAINHFYTGTRLLVKKSSGIKNYADLKGKTVAITTGTTNMQVVRRYNEEKGL
                     GINIVSTKDHADAALLVEADRAAAFAMDDILLFGLKANAKDPASLEVVGDSLQVEPYA
                     CMIRKNDPKFKALVDGVITGMMKSGEFEKLYNKWFMQPIPPRNQSIGLPMSKELQDNI
                     KAPSDKPAT"
     gene            complement(6096..6695)
                     /locus_tag="PKV30_05625"
     CDS             complement(6096..6695)
                     /locus_tag="PKV30_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29731.1"
                     /translation="MTSLTRAPRGRMARSVRRPRVAALWLSACLATAAFAAAPSVPDT
                     ATFVDKASGVTYDYPASFKLDPEFHGSPVMIGVSDSSMEKGDISFRFLTDPALVPKVD
                     SAADAEQLGAALRAQLDASLRRAQFQRMGSTQLLGRPAADVVYRHILNVASRRPLQLR
                     LVATVIGGKTYSLRCSYPADLAAEYDAVCDAIRDSARLR"
     gene            6808..7701
                     /locus_tag="PKV30_05630"
     CDS             6808..7701
                     /locus_tag="PKV30_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL29732.1"
                     /translation="MTSSARPSSSSAAAANGLALGAVGLWASLAALGTSLAHVPPFLL
                     TGLALLLGSVLALPMVRFDVRRLAVPWPTLALGVYGLFGYHFLLFTALQLAPPVEANL
                     VNYLWPLGIVFLAPLLLPGVRLTHLHMVAAVLGFAGAAIAIVGRGGAGASAGPWAAAG
                     YGCAAAAAVVWSTYSLLTKRLSMTGRAFPTAAIGLFGVVSGVLALVCHALLEPSVALS
                     ARDLALIALLGLGPLGGAFFLWDAALKRGDARQIGVLSYLTPLLSTLILMVTTGRPLS
                     WNIALATALILGAAVVGTRAR"
     gene            complement(7788..>8736)
                     /locus_tag="PKV30_05635"
     CDS             complement(7788..>8736)
                     /locus_tag="PKV30_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRL29733.1"
                     /translation="RGRLARLKNTPAVTLDGQRIELLANIEQPSDTAAALGVGAVGVG
                     LFRTEFLFMGREGDLPDEDEQYAAYRAAVEGMHGLPVTIRTVDVGADKPLDDRRPDTH
                     LNPALGLRAIRWSLSEPAMFLTQLRAILRAAAHGRVHLLIPMLAHAGEIRQTLELIDK
                     ARAQLDERGRVYGSVSLGAMIEIPAAALSMRLFLRHFDFLSIGTNDLIQYTLAIDRAD
                     ESVAHLYDQLHPAVLRLVADTIAAGARAGKAVSVCGEMAGDTSVTRLLLGLGLRSFSM
                     HPSQVLAVKQEILRADAGKLQAWAQAVLDSDEPAELLVE"
BASE COUNT         1325 a         2928 c         3023 g         1460 t
ORIGIN      
        1 gcgccaccgg ctggtggcgt ccaccgactg ggacgaggtg caacactggt gccggcagat
       61 ctacctgccg tacgacgcaa agcccgccgg cccggcgcgc cagcccaacg cggtgctgga
      121 cgccatgcag atcgggcagc tcacgctcag ccggttcagc tacggcgtgc cggtgcacct
      181 gagcggcttt gacgaaggcg ccggcattgg cctggtgctg accacgctgc aaggcgcggt
      241 gcggcactgg tccagcggcc gcacgcacgc cgacaccggc gtgggcgact cgtacctggt
      301 cgatctttcg cgcgcgcact accgcctgga cgccgacgcc cggcacctgc aggtcaacct
      361 gacctttcgc cacgacgcca tggccgcgct gcaccagcgc tggttcggct gccccgccga
      421 tgaacgcatg tgggcgcgcg cctaccgctt cggcggcgcg caatcgagct ggatgcagct
      481 gctgggctac gtctgccgct gcatcaccga gatgcccgac gcggtgcagc acggcccgct
      541 ggggcggcac ctggaggaaa tgatcggcgt gcacctgctg acgcaatggc gccagcagct
      601 ggacgcgccc acatcgccca cgctgcacag cctggcgccg cgccacgtgc tgatggccga
      661 gcagcacatt cgcgaccgcg cgcgcgaggc gcccacgctg agcgcgctgg ccagccatgc
      721 gggtgtcagc gtgcgcacgc tcaacgccgc gtttcgcgaa taccgccaat gcacgccgat
      781 gcaggcgctg cgcgacgcgc gcctggctgg cgtgcgcgca gacctgctgg cggcgcggcc
      841 cggcgcgcgc gtgcgcgacg tggccgaggc ctggggctac gcgcagctgg gcatcttcgc
      901 ggccaactac cgccggcggt tcggcgagtc gccttctgaa acactgaatc gccgcgcttg
      961 acgcccggcg gcgctgccgc ggcgaatgac tgtcaaaaga aaacgggctc tagcgcccgc
     1021 ctaatcagcg ctgacagcta tatttattga agcaaaccat catcctgctg cacccgcctg
     1081 catgacaaaa aaaggcccgc cggcgcatga ctgcgcggcg ggccaactcc atccatgccg
     1141 tccggggcag gggtctcaga agaagccgag cttgttctcg ctgtagctga ccagcaggtt
     1201 cttggtctgc tggtagttgt tcagcgccat ctggtggttt tcacggccga tgccggactt
     1261 cttgtaacca ccgaaggccg cgtgcgcggg gtacaagtga tagcagttgg tccacacccg
     1321 gccggcctgg atggcgcggc ccgccttgta ggccacgttg ccattgcgcg accacacgcc
     1381 ggcgcccagg ccgaaagcgg tgtcgttggc gatgtggatg gcttcgtcga aatccttgaa
     1441 ggtggtgacc gaggccacgg gcccgaagat ctcttcctgg aacacgcgca tgtcgttctt
     1501 gccgaacagc agcgtgggct tgacgtagaa gccgtcgttg atgtcgccgc cctggcggtt
     1561 gcgctcgcca cccgtcagag tctgcacgcc ttcattgcgg ccgatgtcga tgtagcccag
     1621 gatgcgctcc atctgctgct gcgacacctg cgcgcccacc atggtgctgg tgtccagcgg
     1681 gtggccttgc ttgatggcgg acacgcgctt gagggcgcgc tcgatgaagc ggtcgtagat
     1741 cgattcttga atcaggatgc gcgacgggca ggtgcaaatc tcgccctggt tcagcgcgaa
     1801 catcgacagg ccttccagcg ccttgtcgag gaaggcgtcg tcgtggtcca tcacgtcggc
     1861 aaagaagatg ttggggctct tgccacccag ctcgacggtg gaggggatca ggttgtcggc
     1921 cgccgcgcgc aggatgcgct ggcccacggg ggtggagccg gtgaaggcta tttttgcaat
     1981 gcgcgtgctg gtggccagcg cctcgcccgc ttccttgccg aagccgttga cgatgttgac
     2041 cacgccgggc ggcagcaggt cgccaatcag ctccatcagc accatgatgg aggcgggcgt
     2101 ttgctcggcg ggcttcatca ccacgcagca gccggcggcc agcgcgggcg gcagcttcca
     2161 cgaagccatc agcagcggga agttccaggg aatgatctgg cccaccacgc cgatgggctc
     2221 atggaagtgg tatgcaaccg tggtttcgtc gatctcggag atgccgcctt cctgcgcgcg
     2281 gatggcgccc gcgaagtagc ggaagtggtc gatggccagc ggaatgtcgg ccgccatggt
     2341 ctcgcgcagc ggcttgccgt tgtcgatggt ttcagcgatg gccagcgtct gcagatgctg
     2401 ctccatgcgg tcggcgatct tcagcaggat gttggagcgc tcggcgaccg aggtcttggc
     2461 ccaggcggcc tgtgcagcgt gggcggcgtc cagcgcgcgg ttcacatcct cggcatcgga
     2521 ctgggccacc ttggtgaagg gcttgccggt gatgggggag acgttctcga aatagcggcc
     2581 ggccgacggg ggcacccatt ggccgccgat gtagttgtcg tattgctgtt tgtagggata
     2641 ggcggcttcg aggccgaaac gcttgagctc aaacatgtcc atggtcttgt ctcctgagag
     2701 gtaggtatgc ggccgcccaa ggcgggtcgg ctgcagacac agatacacag gccgtgccag
     2761 cttggcacac ctgggccaaa ggcgcgggtt taccctgtaa ttggcagccc tatggcttgg
     2821 cttggccccg gggcaatgtc actttatgga acagcgggcg tgacaggccc ggacaatttg
     2881 gaacgtcaca tccgagcagg ggcatttcat accccgcgcc gctccgatag actggcacag
     2941 cccaccgacc gcgcgcttga acgctggtgc gcacgggcac ccgacacccg ccatgccgcc
     3001 cctcggcagg ccgatcagga gacagacagc catgggtatt gcgaccgaca gcctgcagct
     3061 ggcacgccgg cacctgctgg accacggtgc ctgcctgccc agcgcgttga acgaccgcct
     3121 ggcgcgctcc tggcagcgca gcctgcacgc aggcttgccg ccttttggca gccgcgaact
     3181 ggccgaaccc gcaggcggcc tgagcctgtc gcaatccctg gaacaaaacg cccagatgct
     3241 gtccttcgcc cgcccggtga tggactttat gtatgggcag atccgcggcg gcgactgcat
     3301 ggtggtgctg gccgacgtgg acagcatgct gttgcacacg gtgggcgacc acggcttttt
     3361 gaccaaggcg cagcgcgtcg agctgcgcac cggcgcgtcc tggcgcgaat cggtgcgggg
     3421 caccaacgcc atcggcaccg ccattgaaga cgccagcgcg ctgcaggtgc gcgctggcga
     3481 gcactatctg gaacgtaacg ccttcctgca ctgctcggcc gcgcccatct tcaccgcgca
     3541 gggtgccgtg gccggcgcca tcgacatttc gggcgacgag cgcgcatcgc acatgcacgc
     3601 gctggggctg gccagcagcg cggcgcggct gatcgaaaac aactggctca aggcacgtta
     3661 ccgcgatgcg gtgcgcgtgc acctgcacag cgccgccgaa gggctggaca cgccagccga
     3721 gggcatcgtg gtgctgtcag aaagcggcac gctgctgggc gccaaccagg aagcgattcg
     3781 cgccctgcgc ctggcgccgc acgacttctt tgcgcttcag ctggaaacgc gcctgcaaga
     3841 acgcctggcg cacctgctgg cccgcacggg gcgcgaaccg cacaccgtgc gcctgccgga
     3901 cggtcggcgc atgttcttgc gggttgagca acccgcgcag cgcacgcccg cgcgcactgt
     3961 cgttcccgag gctcgcgtgg acgcgctggc agccagcgac acgggcgacg cgcaatggcg
     4021 cagcgccgcg caacaggtgc gccgcattct ggacaagccc atcgcactgc tcatccaggg
     4081 cgaatcgggc gtcggcaagg aagtcttcgc cagggcctgc cacgacagcg ggccgcggcg
     4141 cggccggccc ttcgtggccg tcaactgcgc ggccatcccg gaatcgctga tcgaggcaga
     4201 gctgttcggc tacgcccccg gcgcctacac cggcgcgcaa aagggcggca gcctgggccg
     4261 catccgcgag gccgagggcg gcacgctctt cctggacgag atcggcgaca tgccgctgct
     4321 gctgcaaacc cggctgctgc gcgtgctgca ggaaaaaaag gtcacgccgc tgggcggcac
     4381 gccggtgcct gtggactttg ccttggtcag cgccacgcac tgcgtgctgg ccgacgcggt
     4441 ggcggaaggg cgctttcgca gcgacctgta ctaccgcctc aacggcttca gcgtgaagct
     4501 gcccgccttg cgcgagcgcg aggacttcga tgcgctggcc acgcgcatgc tggcgcagtg
     4561 ggacgacagc ggcgtgcgca tcgcgcccga tctgctggcc gcgctgcgcg gctacacatg
     4621 gccgggcaac ctgcggcagt tggccagcgt gctgcaaacc gcctgcgcgc tgcgcgaccc
     4681 ggcgcacgag ccggccatcg actggccgca cctggccagc gacctgcgcg atgcgctggc
     4741 cagcccggca gcgcgggtgc agcccgccgg taaagcggcg gaaacgcgcg ccatttcggt
     4801 gggccaagct gacggagcac cggaagccgc gccgccggcg gccttgcgaa aaatcgcccg
     4861 ccaggccatc gcgcaggcgc tggccgatgg cggcggcaac gtcagcgagg ccgcccggcg
     4921 cctgggcatc agccgccaga ccatttaccg ccatttgaag aaggccgacg ccgaggcctg
     4981 atctcgcgcg cgcctgccgc ggggcgacgc ctgcattgcg cttttcctga ttgcccagaa
     5041 aaaaggcccg ctcgaagcgg gccttgggcc ttgcgacttt gggtcaaacc atcaggtggc
     5101 cggcttatcg gaaggggcct tgatgttgtc ctgcagttcc ttgctcatcg gcaggccgat
     5161 gctctggttg cgcggcggga tcggctgcat gaaccacttg ttgtagagct tttcaaactc
     5221 gcccgacttc atcatgccgg tgatcacgcc gtcgaccagc gccttgaatt tcgggtcgtt
     5281 cttgcgaatc atgcaggcgt agggctcgac ctgcagcgaa tcgcccacca cttccagcga
     5341 agccgggtcc ttggcgttgg ccttcaggcc aaacagcagg atgtcgtcca tcgcgaaagc
     5401 ggcggcgcgg tcggcctcaa ccagcagggc ggcatcggcg tggtccttgg tggacacgat
     5461 gttgatgccc agcccttttt cctcgttgta gcggcgcacc acctgcatat tggtggtgcc
     5521 ggtggtaatg gccaccgtct tgcccttcag gtcggcgtag ttcttgatgc ccgacgactt
     5581 cttcaccagc aggcgcgtgc cggtgtagaa atggttgatg gcaaagtcca catccttgcc
     5641 gcgggcgctg ttgttggtgg tggagccgca ttccaggtcg atcgtgccgt tttgcagcag
     5701 cggaatgcgg ttggccgagg tcacggcctg ccagcccacg gtgaggttgc ttttgccggt
     5761 ctgcttgcgc accgcgtcgg cgatggcgtt gctgatgtcc accgcaaagc cgatgggctt
     5821 ctccgggcct tccaggtagc tgaacggcac cgacgattcg cggtaggcaa acgtcatctt
     5881 gccgctgctg ctgatcttgg acagcgtgtc ggcgctttgg gcatgggccg gcagcgcggc
     5941 acccagcatg gctacggtgg cggcgcacag ggcgcccgtc aaaacacgtt tcatcgaatt
     6001 caagctccag aagaggtggg tgactacagc ttacagccac ttgtgcgctg cggcgacggg
     6061 gcatgcccta gccaccggcg ccggtcagcg gcccgtcagc gcaggcgggc gctgtcgcga
     6121 atggcgtcgc acaccgcgtc gtattcggcg gccagatcgg ccgggtagct gcagcgcaga
     6181 gagtaggtct tgccaccgat caccgtggca accaggcgca gctgcagcgg ccgccgtgaa
     6241 gccacattaa gaatgtgccg gtacaccacg tcggcggcgg gccgccccag cagctgcgtg
     6301 ctgcccatgc gctgaaactg cgcgcgccgc aaggacgcgt ccagttgcgc gcgcagcgcg
     6361 gcgcccagct gctcggcgtc ggcggcgctg tctacctttg gcacgagcgc tgggtcggtc
     6421 aggaagcgaa agctgatgtc gcctttttcc atcgacgaat cggacacgcc gatcatgacg
     6481 ggcgagccat ggaactccgg gtccagcttg aagctggccg ggtagtcgta ggtgacgccg
     6541 ctggccttgt cgacgaacgt ggccgtgtcg ggcaccgaag gcgcagcagc gaacgccgcc
     6601 gtcgccaggc aggccgacag ccacagcgcg gcgacgcgcg ggcgacgaac cgagcgggcc
     6661 atccggccgc gtggggcgcg tgtgagagag gtcatggcaa gacgactggg cctggcgggc
     6721 ggtggcggaa agccggttat cttacgcaag caatgtccgc ccatcgcccc catcgctgac
     6781 ctacaccgcc ctcgcgccgc gccgcccttg acctcctccg cccgcccttc ctcctcgtcc
     6841 gctgcggcgg ccaatggcct cgccctgggc gcggtgggcc tgtgggcttc gctggccgcg
     6901 ctgggcactt cgctggcgca cgtgccgccg tttttgctga ccgggctggc gctgctgctg
     6961 ggcagcgtgc tggcgctgcc catggtgcgg tttgacgtac ggcggctggc cgtgccttgg
     7021 cccacgctgg cgctgggcgt gtacggcctg tttggctacc acttcctgct gttcaccgcg
     7081 ctgcagctgg cgccgccggt ggaggcgaac ctggtcaact acctgtggcc gctgggcatc
     7141 gtgtttctgg cgccgctgct gctgccgggc gtgcggctga cgcacctgca catggtggcg
     7201 gcggtgctgg gttttgccgg ggcggcgatc gccatcgtgg ggcgcggcgg cgcgggcgcc
     7261 agcgccgggc cgtgggccgc cgcgggctat ggctgcgcgg cggcggcggc ggtggtgtgg
     7321 tccacctatt cgctgctgac caagcgcctg tcgatgaccg gccgcgcctt tcccacggcc
     7381 gccatcggcc tgttcggcgt ggtgtcgggc gtgctggcgc tggtgtgcca cgcgctgctg
     7441 gagcccagcg tggcgctgtc ggcgcgcgac ctggcgctga tcgccctgct ggggctgggc
     7501 ccgctgggcg gcgccttctt cttgtgggac gcggccctca agcgcggcga cgcccggcag
     7561 atcggcgtgc tgagctacct cacgccactc ctgtcaacgc tgatcctgat ggtcaccacg
     7621 ggccggccct taagctggaa catcgcgctg gccacggcgc tgattctggg cgcggcggtg
     7681 gtcggcacgc gcgccaggtg atactccaat ttttatagct gctcgcgctt gatggacaag
     7741 cgcgggtggc caatttggct tgaaatttca ggcgggcgaa ggcggcgcta ttcgaccagc
     7801 aactcggccg gctcgtcgct gtccagcacg gcctgcgccc aggcttgcag cttgcccgcg
     7861 tcggcgcgca ggatttcttg cttgacggcc agcacctgtg acgggtgcat cgaaaagctg
     7921 cgcagcccca gccccagcag cagccgcgtg acgctggtgt cgcccgccat ttcgccgcac
     7981 acgctgacgg ccttgcccgc gcgcgcgccg gcggcaatgg tgtcggccac caggcgcagc
     8041 accgccgggt gcagctggtc gtacaaatgc gccaccgatt cgtcggcgcg gtcgatggcc
     8101 agcgtgtact ggatcaggtc gttggtgccg atcgacaaga agtcgaagtg gcgcagaaac
     8161 agccgcatcg acagcgccgc cgccggaatc tcgatcatcg cgcccaggct gacgctgccg
     8221 tacacgcggc cgcgctcgtc aagctgcgcg cgcgccttgt cgatcagctc cagcgtctgg
     8281 cgaatctcgc cggcgtgcgc cagcatggga atcagcaggt gcacgcgccc gtgcgccgcc
     8341 gcgcgcagga tggcgcgcag ttgcgtgagg aacatcgccg gctccgacag gctccagcgg
     8401 atggcgcgca ggccgagcgc cgggttcagg tgcgtgtcgg ggcggcggtc gtccagcggc
     8461 ttgtcggcgc cgacatcgac ggtgcgaatg gtcaccggca ggccgtgcat gccttccacc
     8521 gccgcgcggt aggcggcgta ctgctcgtcc tcgtccggca ggtcgccttc gcgccccatg
     8581 aacaggaatt cggtgcggaa caagccgacg ccgacggcgc cgacgcccag cgccgccgcc
     8641 gtgtccgatg gctgctcgat gttggccagc agctcgatcc gctgcccgtc cagcgtgacg
     8701 gcgggcgtgt tcttcagccg cgccaggcgg ccgcgc
//
LOCUS       DAOMVO010000080         8718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2082_length_8718_cov_16.805610, whole genome shotgun sequence.
ACCESSION   DAOMVO010000080 DAOMVO010000000
VERSION     DAOMVO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_8718_cov_16.805610"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            640..2031
                     /locus_tag="PKV30_05640"
     CDS             640..2031
                     /locus_tag="PKV30_05640"
                     /inference="COORDINATES: protein motif:HMM:NF037270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29734.1"
                     /translation="MDTRFTDQHRESSVSSSAPGMPLTALSARAERQFATWADFLASG
                     MEATGFHLPSTDPSTQDALLWQIRTSVFWPAWVALDEGAPGSRLAQLADGRQTLSDAR
                     EGAERVARARAALPYGTDGLDLEERVLHFLYLRDGARLQPLCDRHSRLLYRYPLVEAL
                     GANEDQAAQLVQSLQRRRCLEADAPIDRTRHCRQCSGAHPHYFDVCPHCRSIQIERAM
                     ALHCFTCGHVAPQADFQQESALVCPQCHARLRHIGVDYDRPLVQYACADCHHVFIETS
                     VLTRCLDCDAVAAPDALDVRVVAPLRLAARARAMLRQNLQDDLDSDPLTSGYVPIGQF
                     RRMLDWVLAAGRSARSQTFTLVRVATELVDQRPPVLDGEHRQRIVDELAGRIRELLDD
                     ADLCARDAHEHLWLLLPRGDATALVREIQQLVREGSPVRVHVSARRLPDERRPAMPAA
                     QLMAQWQPGEKEG"
     gene            2031..3365
                     /locus_tag="PKV30_05645"
     CDS             2031..3365
                     /locus_tag="PKV30_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL29735.1"
                     /translation="MLTLLNQVAYSFNVLLASMNDRSLAELALKFFPFVLLLELPLFL
                     LVCSGILRYGLRRLREDHQREQYPSVSCIITCYSEGEDVRKTIESLAHQIYRGPIQII
                     AVIDGAIQNGPTLRAALAESDHVAPLHDRALLVLPKWQRGGRVSSLNAGLKMATGQIV
                     MALDGDTSFDNDMVANATRHFDDPDVVAVSGCLRVRNANASLVARLQALEYVLSIGAG
                     KTGLSEFNLVNNISGAFGIFRTRFIRLLGGWDAGTAEDLDITLRIKQYFGRYPHLRIV
                     FEPHAVGHTDAPDTWRGFWRQRLRWDGDLLYLFLRKYRRNIRPRLLGWPNFLFTLVNG
                     VFMQVLMPFVIVLYTFVMLWYLPLGSVLGVLALVYVVYWFSVLILFGLYLIIVSERPG
                     YDLGYLWLTPLLPAFAFANRVHSTFSLLQEASIKAHLDSSMAPWWVLRKTKF"
     gene            3379..5478
                     /locus_tag="PKV30_05650"
     CDS             3379..5478
                     /locus_tag="PKV30_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRL29736.1"
                     /translation="MRRVAAGVVSALFGFLGSVQAQGLSLSQLEAAARENASAVQLRK
                     VETRAAAAEKDAAQALVGARVIGGVGVSDTREPVTTETARQYRRAAASVGVRWPLLGS
                     RLAQQRDADAAQWQESRARLRESIAAEGAVRELRLAIVRGATAGERLGVANAYLQAQS
                     ELEAQLQARQRRGATLTAEVLDLTALSGAVDVARMRQQQALRETKRVVQRLTGYEGDA
                     RVGLPTWPTTCLDPDRLLSADGRGREAMAELDVAAAQAQLDLVGDGIAQSAEAGVQLT
                     HSQSRDFPGRSGHSTGVSVDVSIPMQWQKVRQARRAQWLERKRLASESAQAERALRQE
                     EIERALAERALLQAELDQQRRRLRAAQEGLRVARLRMPALDGDGYAKLWLSRHAVYTA
                     ALQLIDVNERRHVQEIELMALGPACETPPSAEAVSSIDSAALRELASDVGVKPSLSAS
                     LPAQQGRLLGWFVWDGASLLKRPERLDGVASGSGRLLLSFSAREIETLDASAWRALRE
                     RADARQLRLELLLGDPEWVTPSGRAALLQLLQKAQDWPVQGLNLDIERSQLRSTMTAD
                     TWHREVLATLGAVRAAVPWHLSLTTHHRDLDDAEFLDALKASGVDEAVAMVYTTREAT
                     ARATIERLLAKTARGPRIALAQSVESALPANESSFLKGRSASLTIWRNLVTALSPNQR
                     FSGLIVQSLADYEKARP"
     gene            5475..6557
                     /locus_tag="PKV30_05655"
     CDS             5475..6557
                     /locus_tag="PKV30_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRL29737.1"
                     /translation="MKIRFDTPPARADQAGDLSVRYAAAKRTVPRWRWRLVLLLLALT
                     PAYFVIRALSSFFWETAPGLVVMQSTVVRAGVSGHVTFLAESGARLARGDVLARVQPA
                     NAATPAPPLSSAGVAPPLTGTPRMDERSAPLQQALRVAEQQRRLAQDRLKLMQSLRAQ
                     DAATEQEVQSARAAALQADSAWVRTRLDLAELQEQAHQNGLKVAPPPLSGASASSVNA
                     TRKEQLVRMPFDGVVSAPQVAEGEWISASTDLAALLSTAAPMIHAYLAPSDIQYAAKG
                     RHATLIFQDGGRLPAEVLGVTAEAERQPAESVSPLVPRNLSVVVRLRPVDPLPDRYRI
                     FRLPLEVRFDRSWREQGSGWLRPLTG"
     gene            complement(6554..7606)
                     /gene="galE"
                     /locus_tag="PKV30_05660"
     CDS             complement(6554..7606)
                     /gene="galE"
                     /locus_tag="PKV30_05660"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRL29738.1"
                     /translation="MTPGHVLVTGGAGYIGSHTCLALLQAGWRVTVADNLSTGSREAL
                     RRVEQLTGLPLALAQVDVRDAAAVQALLEAHDFDAVVHFAALKVLSESLRDPLSYYDH
                     NVHGLTVLLQAMRRANVRSLVFSSSAAVYGVPDSVPIREDAPLLGVNPYAVTKRIGEE
                     MLGHLVASEPGWRAVSLRYFNPVGAHESGLLGESPAATPSNLMPVICDVALGRRAELP
                     VFGTDYPTRDGSCIRDFIHVMDLAQGHVRALHWMAEQPAPQCVAVNLGTGRGHSVLEM
                     VQAFQAANGVAVPYRVVARRPGEVADCHADARLAQSLLGWQAERGLAAMCTDAWRWSQ
                     SHPGGFARAGSKAAAV"
     gene            complement(7610..8617)
                     /gene="lipA"
                     /locus_tag="PKV30_05665"
     CDS             complement(7610..8617)
                     /gene="lipA"
                     /locus_tag="PKV30_05665"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRL29739.1"
                     /translation="MSTPLSGPHAPTVRDAQPADSYDATAKQKAGAKLSRIPVKVQNT
                     GPALKKPEWIRVKAGSPSTRFYEIKDILRKNNLVTVCEEASCPNIGECFGHGTATFMI
                     MGDKCTRRCPFCDVGHGRPDPLDANEPVNLAGTIAQLKLKYVVITSVDRDDLRDGGSQ
                     HFVDCIRETRARSPQTQIEILTPDFRGRDDRALEILKAAPPDVMNHNLETVPRLYKEA
                     RPGSDYAFSLNLLKKFKALHPGVPTKSGLMVGLGETDEEILDVMRDLRAHGVDMLTIG
                     QYLAPSNAHLSVKRYVHPDTFKMFEREAYAMGFTHAAVGAMVRSSYHADQQAQGVLNA
                     T"
BASE COUNT         1373 a         2701 c         3003 g         1641 t
ORIGIN      
        1 ctaggcgggg cgtggcccag cgttgtctga aactggcaag gcggtgtctc tgcatggcag
       61 gaatggaggc tgcggcaagc cgacaccggc ttacgagccg gcacggtcaa gcctttccgg
      121 gcgaacgata gaacgctgcc ggcgcgcgcg ccgaacgcgg ccggcagagc ggaggggctg
      181 tggcgcgtct tagcgctcgg cacttgggtc atggccctgt tcaggacttc ttggcggcgc
      241 acgagcgcgc ccacagcaac atggaacaag cccttggggt agctcgacga gcgaacacct
      301 caaagggtgt gagggacaag cgctgacagc gcgatttgtc gtgacttctg gtgccttaaa
      361 gcaaccaaaa aatgtctgtt cgataaccga caatcgatgt gctggatggc caacgtccta
      421 gcgaacggct ttttgcgact tttttcactg tcggctgacc aagatgcgga gccttgttca
      481 acaccctgtg cgatgctcgg gatgtttttt tggcggcgca gtcggtgcca gggtcacttg
      541 tgtgggcgag gcgatgttgc gttccaggaa ccgccagttg ttcacactta catggccata
      601 ggtgcgacgc gccgatcttg tcgctgtcgc cccaggttca tggatacccg ttttaccgat
      661 cagcatcgag agtcaagtgt ctcgtccagc gcgcctggca tgccgctgac ggcgttgtcc
      721 gcccgggccg agcggcagtt tgcaacgtgg gcggactttc tcgccagtgg catggaggcg
      781 acgggttttc atctgccgtc gaccgacccc tccactcagg acgcattgct gtggcagatc
      841 cgcacctcgg tgttctggcc agcgtgggtt gcactcgatg aaggcgcgcc ggggtccaga
      901 ctggcgcagt tggcggacgg gcggcagacg ctttccgatg cgcgcgaggg ggccgagcgt
      961 gtggcgcgcg cgcgcgccgc cttgccgtac gggacggatg ggttggatct tgaagagcgc
     1021 gtgcttcact ttctgtatct gagggatggc gcccgtttgc aaccgctgtg cgaccgccac
     1081 agtcggctgc tgtaccgcta tccgttggtg gaagcgctgg gtgcgaacga ggatcaggcg
     1141 gcacagctgg tgcaatcgct gcagcgtcga cgttgtctcg aagccgacgc accgattgac
     1201 cgtacgcggc attgccgtca gtgcagcggc gcccatccgc actacttcga cgtctgtcca
     1261 cattgcagga gcattcagat cgagcgcgcg atggcgctgc actgctttac ctgtgggcac
     1321 gtggcgccgc aggcggactt tcagcaggag agcgcgctgg tctgcccgca gtgccatgcg
     1381 cggctgcgcc acatcggtgt ggactatgac cgaccactcg tgcagtacgc ctgcgcggat
     1441 tgccatcacg ttttcataga gacatcggtg ctcacgcgct gtctggactg cgatgccgtc
     1501 gcggcacccg atgcgctgga tgttcgagtc gtcgcgccat tgcggctggc ggcgcgcgcg
     1561 cgcgcgatgc tgaggcaaaa cctgcaggac gacctggaca gtgacccgct gacgagcggg
     1621 tatgtgccga tcgggcagtt tcgtcggatg ctggattggg tgctggcggc cggccggagt
     1681 gcgcgatcgc agacctttac gctggtccgg gtggcgacgg agctcgtcga tcaacgaccg
     1741 ccggtgctgg acggcgagca ccgacagcgg atcgtggatg aactcgccgg gcgcatccgt
     1801 gaactgttgg acgacgccga tctttgcgcg cgcgacgcgc acgagcatct gtggctgctg
     1861 ctgccacgcg gcgacgccac cgcgttggtt cgtgagattc aacaactggt tcgcgaggga
     1921 tcccctgtgc gggtgcacgt cagcgcgcgc cgccttcctg atgaacggcg tccggcaatg
     1981 cctgcggcgc aacttatggc gcaatggcag cccggagaaa aggagggctg atgctgacgc
     2041 tgttgaacca ggtcgcctat tcgttcaacg tgctgctggc gagcatgaac gatcgcagtt
     2101 tggccgagtt ggcgctgaag tttttccctt tcgtcctgtt gctcgaactg ccgctgtttc
     2161 tgttggtctg tagcggcatt cttcgctacg gtctgcgccg gctgagggaa gaccatcagc
     2221 gggagcaata ccccagcgtc tcctgcatca tcacctgcta ttcggaaggc gaggacgttc
     2281 gcaagacgat cgagtcgctg gcgcaccaga tttaccgcgg gcccattcag atcatcgccg
     2341 tgatcgacgg cgccatccag aacgggccga cgttgcgcgc cgcgctggcg gagtcggacc
     2401 atgtcgctcc attgcacgat cgggcgttgc tggtcttgcc gaaatggcaa cgcggcggtc
     2461 gcgtgtcctc attgaatgcg ggtttgaaga tggcgaccgg ccagatcgtg atggcgctgg
     2521 acggcgatac ctcgtttgac aacgacatgg tggccaatgc cacccgtcac tttgatgatc
     2581 ctgacgtcgt tgctgtctcg ggttgcctgc gcgtacgcaa cgccaacgcc tcgctggttg
     2641 cgcgcttgca ggcactggag tacgtgcttt ccataggggc gggaaagacg ggactgtccg
     2701 agttcaacct ggtgaacaac atttccggcg ctttcggcat tttccgcacc cgcttcattc
     2761 gcttgctggg aggatgggat gccggcaccg cggaggatct ggacatcacc ttgcgcatca
     2821 agcagtactt cgggcgctac ccccatctgc gcatagtctt tgaaccccat gcggtggggc
     2881 ataccgatgc ccccgatacc tggcgcgggt tctggcgcca gcgcctgcgc tgggacggtg
     2941 acctgcttta cctgttcctg cgcaagtacc ggcgcaatat ccgtcctcgt ctgttggggt
     3001 ggcccaactt cctgttcacg ttggtcaatg gcgttttcat gcaggtgctg atgccgtttg
     3061 tcatcgtgct ctacaccttt gtgatgctgt ggtatctgcc gctgggttcg gtgctggggg
     3121 tgctcgcctt ggtctatgtg gtctattggt tctcggtttt gatcttgttc gggctctacc
     3181 tcatcatcgt gtcggagcgc cctggctacg acctcggcta cctgtggttg acgccgctgt
     3241 tgccggcatt cgcctttgcc aatcgcgtgc acagcacctt ctctctgttg caggaagcgt
     3301 ccatcaaggc gcacttggat tcctccatgg cgccttggtg ggtgctgcga aagaccaagt
     3361 tttgaaggcg acaagaccat gaggcgagtc gctgcgggcg tggtcagcgc gctgtttggt
     3421 tttttgggca gcgttcaagc gcaaggcctg tcgctttcgc aactggaagc ggcggcgcgt
     3481 gaaaacgcca gtgcagtgca gctcagaaag gtcgaaaccc gggcggcggc ggccgagaag
     3541 gatgcggcgc aggcgctggt aggtgcacgg gtgattggtg gcgtcggcgt cagcgacacg
     3601 cgcgagcccg tgaccacaga aactgcccgc cagtaccggc gagctgctgc cagcgtaggc
     3661 gtgcgctggc cgttgctggg cagtcgtctg gcccagcagc gggatgccga cgccgcgcaa
     3721 tggcaggaga gccgggcccg cttgcgtgaa agcattgcgg ccgagggcgc ggtgcgcgag
     3781 ttgcgcctgg cgatcgttcg aggggcgacg gccggtgaac ggctgggcgt ggccaatgcc
     3841 tatctgcagg cccagtcgga gctggaggcg cagttgcagg cgcgccagcg gcgtggcgcg
     3901 acgttgaccg cggaagtgct ggacctgacg gccttgagcg gcgcggtgga cgtggcgcgg
     3961 atgcggcagc agcaggcctt gcgcgagacc aagcgggtgg ttcaacgcct gacgggctac
     4021 gaaggcgacg cgagggtcgg cctgccgacc tggccgacca cctgcctgga ccccgaccgc
     4081 ttgctcagcg ccgatggccg tgggcgcgaa gccatggccg agctcgacgt cgcggccgca
     4141 caggcgcaac ttgatctggt tggtgacggg atcgcccaga gcgccgaagc gggcgtgcag
     4201 ctcacccatt cgcagagccg ggattttcca ggccgcagtg gtcacagcac cggcgtttcg
     4261 gtggacgtga gcatcccgat gcagtggcag aaggtgcgtc aggcgcggcg cgcgcaatgg
     4321 ttggagcgca agcgtctggc cagcgagtcg gcgcaggccg aacgcgcgct tcggcaagaa
     4381 gagattgagc gcgcgcttgc tgagcgagcg ctgctgcaag ctgaactcga tcaacagagg
     4441 cgccgcttgc gtgcggcgca ggagggcttg cgcgtggcgc gcctgcgcat gcccgcactg
     4501 gatggcgatg gctatgccaa gctgtggctg tcgcgtcatg cggtctacac cgctgcgctg
     4561 cagctgatcg atgtgaatga gcgccgtcac gtgcaggaga ttgaactcat ggccctgggg
     4621 ccggcgtgcg agacgccccc atccgctgaa gcggtatcga gcatcgattc agcggccttg
     4681 cgcgagcttg cgtccgatgt gggtgtgaaa ccatcgctct cggcatcgct gcccgcgcag
     4741 caggggcggt tgctagggtg gtttgtgtgg gacggcgcgt ccttgctcaa gcgtccggaa
     4801 cgcctggatg gagttgcctc tgggtcgggt cgactgctgc tgtcgttcag cgcccgcgag
     4861 atcgagaccc tcgacgcatc ggcatggcgt gcactgcgcg aacgtgctga cgccaggcaa
     4921 ttgcgtctgg agctgttgtt gggcgatccc gaatgggtga cgccgtcggg acgcgcggcg
     4981 ctgctccagc tgctgcaaaa agcccaggat tggccggtgc aaggtttgaa cctcgacatc
     5041 gagcgcagtc agttgcgcag caccatgacc gcggacacct ggcaccgcga ggtgttggcg
     5101 acgctcggtg cggtgcgggc cgccgtccct tggcatctga gtctgaccac ccaccatcga
     5161 gacctcgatg atgccgagtt cctggatgcg ctgaaggcga gcggcgtgga cgaggcggtc
     5221 gccatggtct acaccacgcg ggaggccaca gcacgagcca cgatcgagcg cttgcttgcc
     5281 aagaccgcgc gcgggccacg cattgcgctc gcacagagtg tcgagtccgc gctgccggcg
     5341 aatgagagca gttttttgaa gggacgatcg gcctccttga ccatctggcg gaacctggtg
     5401 acggcgctgt ccccgaatca gcggttcagc ggattgatcg tccaatcgct ggccgactat
     5461 gaaaaggctc gtccatgaaa atccgtttcg acacaccgcc tgcgcgcgcc gatcaggccg
     5521 gcgacctgtc ggtgcgttac gccgcggcga agcgcaccgt cccgcgttgg cgttggcgcc
     5581 tcgtgctgtt gctgctggcc ctgacgcccg cttatttcgt gatccgtgcg ctctcgtcct
     5641 ttttttggga gactgcgccg ggcctcgtgg tcatgcaaag cacggtggtg cgcgccggtg
     5701 tgagtggcca tgtcacgttc ctggccgaga gtggggcgcg cctggcgcgc ggcgacgttt
     5761 tggcgcgggt gcagccagcc aacgcggcca cgcctgcgcc ccccttgtcc tctgccgggg
     5821 tcgccccacc gttgactggc accccgcgga tggatgagcg ttcggcgcct ttgcaacagg
     5881 ccttgcgcgt ggccgaacaa cagcggcgcc tggcgcagga ccggctgaaa ctgatgcagt
     5941 ccttgcgcgc gcaggacgcc gccaccgagc aggaggttca gtcggcgcgg gcggcggcct
     6001 tgcaggccga ttcggcatgg gtgcggacgc ggctggacct ggccgagctg caggagcaag
     6061 cccatcagaa cggcttgaag gtggcgccgc cgccgctcag cggcgcgtcc gcgtcctccg
     6121 tcaacgcgac gcgcaaggag cagcttgtcc gcatgccatt cgatggtgtg gtgtccgcgc
     6181 cgcaggtggc cgaaggtgag tggatatcgg ccagcacgga tctggccgcg ctgctcagca
     6241 ccgccgcgcc gatgatccac gcctacctgg cgcccagcga cattcagtac gcggccaagg
     6301 ggcgccacgc caccttgatt tttcaggatg gtggccgcct gcccgccgag gtgttgggcg
     6361 tcacggccga agcggagcgc caacctgcgg aatcggtttc gccgctggtg ccgcgcaacc
     6421 tgtccgtggt ggtgcgcctg cgtcccgtgg atccgctccc ggatcgctac cgcatctttc
     6481 gcttgccgct ggaagtgcgg ttcgaccgct cctggcgcga acagggctcg ggctggctgc
     6541 ggccgctcac aggctagacc gcggcggctt tgcttccggc gcgcgcgaag ccgccggggt
     6601 gactctggct ccagcgccag gcgtcggtgc acatggcggc gaggccgcgc tcggcctgcc
     6661 aacccagcag cgattgggcg agccgcgcat cggcgtgaca gtccgccact tcccccggcc
     6721 gtcgcgccac cacacggtag ggcacggcga cgccgttggc cgcttggaac gcttgaacca
     6781 tctccagcac agagtggccg cggcccgtgc ccaggttgac cgcgacacac tggggtgccg
     6841 gttgctcggc catccagtgc aaggcgcgca catgcccctg ggccaggtcc atcacgtgga
     6901 tgaaatcgcg gatgcagctg ccatcgcgcg tcgggtaatc ggtgccgaag acgggcagtt
     6961 cggcgcggcg gcccagtgcg acgtcgcaaa tgaccggcat caggttgctg ggcgtcgccg
     7021 cgggcgattc gccgagcagc ccgctttcgt gtgcgcccac cgggttgaag taacgcagtg
     7081 acacggcccg ccagccaggc tccgacgcca ccagatgccc caacatctct tcaccgatcc
     7141 gcttggtgac ggcgtagggg ttcacgccaa gcagaggggc gtcctcacgg atgggcacgg
     7201 aatcgggtac gccatacact gcggcggacg aggaaaacac caagctgcga acgttcgcgc
     7261 gtcgcatcgc ctgcaacagc acggtcagcc catgcacgtt gtggtcgtag tagctgagtg
     7321 ggtccctgag cgactcggac aacaccttca gcgccgcgaa atggaccacc gcgtcaaagt
     7381 catgggcctc cagcagcgcc tgtacagcgg cggcgtcgcg cacatccacc tgcgccaatg
     7441 ccagtggcag accggtcagc tgctccaccc ggcgcagtgc ctcgcgcgaa cccgtgctca
     7501 ggttgtccgc aacggtcacc cgccaaccgg cctgcagcag cgccaggcag gtgtgcgatc
     7561 cgatgtaacc ggcgccgccg gtgaccagta catgcccggg cgtcatgcct caggtggcgt
     7621 tcagcacgcc ctgcgcctgc tggtcggcgt ggtaactgct gcgcaccatg gcgcccacgg
     7681 cggcgtgggt gaagcccatg gcgtaggcct cgcgctcgaa catcttgaag gtgtcggggt
     7741 gcacgtagcg cttgaccgac aggtgcgcgt tgctgggcgc caggtactgg ccgatggtca
     7801 gcatgtcgac gccgtgcgcg cgcaggtcgc gcatcacgtc cagaatctct tcgtcggttt
     7861 cgcccaggcc caccatcagg ccgctcttgg tcggcacgcc cgggtgcagg gctttgaatt
     7921 tcttcagcag attcaggctg aaggcgtagt cgctgccggg gcgcgcttcc ttgtacaggc
     7981 gcggcaccgt ttcgaggttg tggttcatca cgtcgggcgg cgcggctttc aaaatctcca
     8041 gcgcgcggtc gtcgcggccg cggaagtcgg gcgtcaggat ttcgatctgc gtctgcggcg
     8101 aacgcgcgcg cgtctcgcgg atgcaatcga caaagtgctg gctgccgccg tcgcgcaaat
     8161 cgtcgcggtc cacgctggtg atgaccacgt atttcagctt gagctgcgcg atggtgcccg
     8221 ccagattgac cggctcgttc gcatccagcg ggtcgggccg gccgtggccc acgtcgcaga
     8281 acgggcagcg gcgcgtgcac ttgtcgccca tgatcatgaa ggtggccgtg ccgtggccaa
     8341 agcactcgcc gatgttgggg cagctcgcct cttcgcacac cgtcaccaag ttgttcttgc
     8401 gcaggatgtc cttgatttcg tagaagcgcg tgctgggcga gccggccttg acgcgaatcc
     8461 actcgggctt tttcaacgca gggccggtgt tttgcacctt gaccgggatg cggctcagct
     8521 tggcgccggc tttttgcttg gcggtggcgt cgtagctgtc agccggttgc gcgtcgcgca
     8581 cggtgggcgc gtgagggcca gagagggggg tggacatgcg atcaggctct ttctcaaacc
     8641 gccagcaggc gctcaagctg cgtggccagt tcgtgggcgg cctcatccca tgaagccgaa
     8701 acgccgattg tagaaaga
//
LOCUS       DAOMVO010000081         8706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2088_length_8706_cov_12.602936, whole genome shotgun sequence.
ACCESSION   DAOMVO010000081 DAOMVO010000000
VERSION     DAOMVO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_8706_cov_12.602936"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PKV30_05670"
     CDS             <1..1502
                     /locus_tag="PKV30_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HRL29740.1"
                     /translation="LEEPPEYLKFVLATTDPQKVPVTVLSRCLQFNLRPMAPETVLQH
                     LTHVLAAENVAAEPQALRLLARAARGSMRDALSLTDQAIAFGSGQLDEATVRQMLGSV
                     DRSVVFSLIGALAAGDGRAVVELSDQLRAAGLSAATALEDMASVLQRMAVHQAVPGMA
                     VDDTDPDAQETARLAALLPADETQLLYSLCIHGRAELGLAPDEYAGLTMVLLRLLPFK
                     HAGGAPAEKKTLKSPEPRPAPAVAAAQPAVPAPQPQASVTSAPASPAAAEDPPADDRG
                     FAPPDAEPADEPFFVADDAEGDVHLPPAPQRAHPPLQPMPVRQLPARPAFEENRPPVQ
                     AGQAQEATEIVAVKVREGADSQQPRPRAVLDAPPGVVPGAHGDFWHTLVQDLVAREAV
                     AALVRELALQSDLVARAGDQWTLRVESETLGQSGARDRLQAALADAGHAVRLQVEIGP
                     VADSPSRRNAIAAAQRLKAAEALLMADPFVQEMVRDFGAKIVPGSIKPL"
     gene            1556..1891
                     /locus_tag="PKV30_05675"
     CDS             1556..1891
                     /locus_tag="PKV30_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRL29741.1"
                     /translation="MFNKGQLAGLMKQAQAMQDNLKKAQDELALMEVEGESGAGLVKV
                     LMTAKHDVKRVTIDPSLLADDKDMLEDLVAAAFNAAVRKAEELSAEKMGKLMPAGMPG
                     MPPGMKFPF"
     gene            2012..2602
                     /gene="recR"
                     /locus_tag="PKV30_05680"
     CDS             2012..2602
                     /gene="recR"
                     /locus_tag="PKV30_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRL29742.1"
                     /translation="MPDTHALDTLIQSLRRLPGVGVRSAQRMAFHLLQHDREGAQVLA
                     RALQEAVAQVRHCARCHTFTESEICATCADPRRDASKLCVVETPADQAAMERTAAFKG
                     RYFVLMGKLSPLDGIGPREIGVQKLIERATDGVVQEVILATNFTAEGEATAHVIGQAL
                     KSRGLNVTRLARGVPAGSELEYVDLGTIAHALVDRR"
     gene            2675..3097
                     /locus_tag="PKV30_05685"
     CDS             2675..3097
                     /locus_tag="PKV30_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRL29743.1"
                     /translation="MNSSSLHSFSIAHWCILIAALLPLLSSYIAKWGQLGKPRRDGGY
                     DNADPRAWLARQGDWRARANAAQANSFEGLPFFIGAVLAALQLGAPQGRVDLLALLYV
                     MLRLGYIVAYVGGMATMRSAVWALAFAVNVAILFQGWR"
     gene            3261..4298
                     /locus_tag="PKV30_05690"
     CDS             3261..4298
                     /locus_tag="PKV30_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL29744.1"
                     /translation="MGGRRAVLRGLAAATLASCALVRAQERPEKPKLSIAVGGRTTVY
                     YLPLTIADQLGFFAHEGLEVDLQDHAGGGLALQSLLQGRADVAAGGFEHTILLRQRGV
                     NCRAFALLGRAPQLVFGVSTRALPEFRDPSQLKGRRVGISAPDSSTHWFARLVLARAG
                     LAATDVEYVGVGTSTAAVMALREGRIDAIASIDPVISMLEFRGEIRVLADTRSPRGTQ
                     ELYGGPMPGGCIYAPQAFVVRYPQTVQALTNAAVRALKWLQTAGPSDIVRAVPESYMY
                     GDRAIYLASLEKARTALCPDGLMTEEAVATAHRVVAQYYSGSAPVRPQAPGATYTNDF
                     ARRAKLKFQVA"
     gene            complement(4333..6045)
                     /locus_tag="PKV30_05695"
     CDS             complement(4333..6045)
                     /locus_tag="PKV30_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL29745.1"
                     /translation="MKWYQGLALVVSVALAGCAAPGGGADGASDGASAATSTARTAPR
                     MPRSGEMPDVAVSELRGGQVAALEAPADLWERVRRGFAMPDLQGELVERHEQWYTTRP
                     DYIERMVDRSRLYIFHIVEELELRGMPTELALLPYIESAFNPQAVSSAKAAGMWQFMP
                     GTGRDFQLTQNMLRDDRRNVLGSTRAALDYLQKLHDMFGDWHLALAAYNWGQGNVKRA
                     IERNQAAGLPTGYNDINMPAETRNYVPKLQAVKNIIANPQRMGAVLPLIENHPFFDTV
                     DITQDIDVDVAARLAEVRVEDFKALNPSQRKPVIFAAGTPQILLPWNNADTFKQNLAK
                     AAPGSLASWTAWVATDTLPSRAVASRFGMDEDDFREMNNIPRGMVIKSGSTVVVRRSN
                     GAAQAVASHVVNNAQLAYTPEIVLRRTSVRARKGDSIASVAARYDLPAATVAGWNHTN
                     ARAGLKRGQAVVLYLPVRAAAAAARDGVEAAARRERSQTATRGAVRASHLSESRAERA
                     KASGADKGKAKNQRAGKNDRRADAKPASKSAKPSPKDSPGKSAGKGGAKAKAEPKASG
                     KNKR"
     gene            complement(6042..6812)
                     /gene="gloB"
                     /locus_tag="PKV30_05700"
     CDS             complement(6042..6812)
                     /gene="gloB"
                     /locus_tag="PKV30_05700"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRL29746.1"
                     /translation="MELIALPAFNDNYLWLLHDGQQALVVDPGDAAPVRAALERLALR
                     LASILVTHHHADHTGGVAELRAETGAQVFGPARERIPEPYTPLQGGDVVQTLGVSFRV
                     IDVPGHTAGHIAYFADDVAGAPLLFCGDTLFSGGCGRLFEGTPAQMLASLDALAALPG
                     DTRVCCAHEYTLGNLRFARAVEPGNQALADYEQHCQRLRAGGQPTLPSSIGTERAINP
                     FLRSREPAVIQSVRSHAPQATDEVAVFAALRQWKNEFK"
     gene            6898..7722
                     /locus_tag="PKV30_05705"
     CDS             6898..7722
                     /locus_tag="PKV30_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL29747.1"
                     /translation="MHEWFQTPPGQYVLAWERAQFDAALADVFGYHALQIGLADIDAL
                     AANRMPHRWLAMTSAPGAPDEDPTPATVAAGDEAPPPPRIALVTDPTALPFAEASLDL
                     VVLPHTLELSADPHASLREVQRVLVHEGRVAIAGLNPASLWGLRQYRAHLLRGGGQGQ
                     LYLPDAGEFIGHWRLRDWLRLLQFELESLSFGCYAPAVRSARNLARFGWMDRVGARWW
                     PIFGAAYFMVAVKRSAGPKLVGAAWKKSPIPANAPVSIAGRAGLASAGGQKDHESH"
     gene            7709..8149
                     /gene="rnhA"
                     /locus_tag="PKV30_05710"
     CDS             7709..8149
                     /gene="rnhA"
                     /locus_tag="PKV30_05710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRL29748.1"
                     /translation="MNRIEMYTDGACKGNPGPGGWGVLLRAPGLEKELFGGEPLTTNN
                     RMELTAVIEGLQALKRPCEVDLYLDSQYVRQGITEWVAGWKARGWRTSTKQPVKNVDL
                     WQKLDALVAEGGHRIQWHWVRGHAGHEGNERADALANRGVPGRR"
     gene            8255..>8706
                     /locus_tag="PKV30_05715"
     CDS             8255..>8706
                     /locus_tag="PKV30_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRL29749.1"
                     /translation="MKLSTSLIAAVALAGAIGAAWWFQRPSQSKPATDGPARVASAPA
                     AGGTAAGPVVVESALVRVRPLSEDVEAVGSLRSRQGTVVRAEVGGRVTQINFRDGQRI
                     RRGQLLVQLDDRLQRAQVQQAQAELSIAETNHRRNSELVSKGFISRRGV"
BASE COUNT         1294 a         3014 c         3053 g         1345 t
ORIGIN      
        1 cgctggagga gccgcccgaa tacctcaagt tcgtgctcgc caccaccgat ccgcaaaagg
       61 tgccggtgac cgtgctgtcg cgctgcctgc aattcaacct gcggccgatg gcgcccgaga
      121 cggtgctgca gcacctgacc cacgtgctgg ccgccgaaaa cgtggcagcc gagccgcagg
      181 cgctgcgcct gctggcgcgc gcggcgcgcg gctcgatgcg cgacgccctc agcctgaccg
      241 accaggccat cgccttcggc tccggccagt tggacgaggc cacggtgcgc cagatgctgg
      301 gcagcgtcga ccgcagcgtg gtgttcagcc tgatcggcgc gctggcggcg ggcgacggcc
      361 gggcggtggt cgagttgtcc gaccagttgc gcgcggctgg cctgtcggcc gccaccgcgc
      421 tggaagacat ggccagcgtg ctgcagcgaa tggccgtgca ccaggccgtg cccggcatgg
      481 cggtggacga caccgacccc gacgcgcagg aaaccgcgcg cctggccgcg ctgctgcctg
      541 ccgacgagac gcaactgctg tacagcctgt gcatccacgg ccgcgccgag ctgggcctgg
      601 cgcccgacga atacgcgggc ctgaccatgg tgctgctgcg cctgctgccc ttcaagcatg
      661 cgggtggcgc cccggctgaa aaaaaaactc tgaaatcgcc tgaaccgcgg cccgcgccgg
      721 ccgtggcggc ggcgcagcca gcggtgcctg cgccccagcc gcaggccagt gtgaccagcg
      781 cgcctgcgtc gcccgcagcc gctgaagacc ctcccgccga tgaccgcggc ttcgccccgc
      841 cggacgccga gccggccgat gagcccttct tcgttgccga cgacgcggaa ggcgatgtcc
      901 atctgccccc cgccccgcag cgcgcgcacc cgccgctgca gccgatgccc gtgcgccaac
      961 tgcctgcgcg cccggctttt gaagaaaatc ggccaccagt gcaagcagga caagcgcagg
     1021 aagctactga aatagtagcg gtcaaggtgc gcgaaggcgc cgattcgcag caaccccggc
     1081 cgcgcgccgt gctcgatgcg ccacccggcg tggtccccgg cgcgcacggc gacttctggc
     1141 acacgctggt gcaggacctg gtcgcgcgcg aagcggtggc ggcgctggtg cgcgaactgg
     1201 ccctgcagtc cgacctggtg gcgcgcgctg gcgatcaatg gaccttgcgt gtggaaagcg
     1261 agacgctcgg ccaatcgggc gcgcgcgacc gcttgcaggc tgcgctggcc gacgccggcc
     1321 acgccgtgcg cctgcaggtc gagatcggcc cggtggccga cagcccttcg cgccgcaacg
     1381 ccattgccgc cgcccagcgc ctaaaggccg ccgaggccct gctcatggcc gaccccttcg
     1441 tgcaggagat ggtgcgcgac tttggggcga aaatcgtacc cggcagcatc aagccgctgt
     1501 gaccgcgggt cgtggcgaca acgcactgat tttttgacga caaggaaagc attcgatgtt
     1561 caacaaagga caactcgccg gcctgatgaa gcaggcccag gccatgcagg acaacctgaa
     1621 gaaggcgcag gacgaactgg cgctgatgga agtggaaggc gaatccggcg ctggcctggt
     1681 caaggtgctg atgaccgcca agcacgacgt caagcgcgtc accatcgacc ccagcctgct
     1741 ggccgacgac aaggacatgc tcgaagacct ggtcgccgcc gccttcaacg ccgccgtgcg
     1801 caaggccgag gagctgtcgg ccgagaagat gggcaagctg atgccggccg gcatgcccgg
     1861 gatgccgccc ggcatgaagt ttccgttttg acccgatgac caaatgacct cggcctgcgg
     1921 ccttcgatga cggcgcggcg cgccatgacc gcgttggcgt catttcgtca ttgaagccgc
     1981 gccagcggcg aagtcattgc gtcattcaaa catgcccgac acccacgccc tcgacacgct
     2041 gatccagtcg ctgcggcgcc tgcccggcgt cggcgtgcgc tcggcgcagc gcatggcgtt
     2101 tcatttgctg cagcacgacc gcgagggcgc gcaggtgctg gcgcgcgcgc tgcaggaggc
     2161 ggtagcgcag gtgcgccatt gcgcgcgctg ccacaccttc accgaaagcg agatctgcgc
     2221 cacctgcgcc gatccgcggc gcgacgcgag caagctgtgc gtggtcgaga cgccggccga
     2281 tcaggcggcg atggaacgca cggccgcctt caagggccgc tacttcgtgc tgatgggcaa
     2341 gctcagcccg ctcgacggca ttggcccgcg cgagatcggc gtgcaaaagc tgatcgagcg
     2401 cgccaccgac ggcgtggtgc aggaggtgat cctggccacc aacttcaccg ccgagggcga
     2461 agccaccgcg cacgtcatcg gccaggcgct gaaaagccgc ggcctcaacg tcacgcggct
     2521 ggcgcgcggc gtgccggcgg gcagcgagct ggaatacgtc gacctgggca ccatcgcgca
     2581 tgcgctggtg gaccggcgct gacccatttt caggttactc ttgtttttat agctgctcgc
     2641 gcttgtcaat caagcgctgg cggccttttt tgatatgaat tccagctctc tccacagttt
     2701 cagcatcgcc cactggtgca tcctgatcgc ggcgttgctg ccgttgttga gttcctacat
     2761 cgccaagtgg ggccagctcg gcaaaccgcg ccgcgacggc ggctacgaca acgccgatcc
     2821 gcgcgcctgg ctggcgcgcc agggcgactg gcgtgcgcgc gccaacgcgg cgcaggccaa
     2881 cagcttcgag ggcttgccgt tcttcatcgg cgcggtgctg gcggccctgc aactgggcgc
     2941 gccgcagggt cgggtcgatc tgctggcgct gctgtacgtg atgctgcggc tgggctacat
     3001 cgtggcgtac gtgggcggca tggcgaccat gcgcagcgcg gtctgggcgc tggcgttcgc
     3061 ggtcaacgtc gcgatcctgt ttcagggctg gcgctgagct tgtaagcggg tgtcaccgac
     3121 gtaccctgct tggtggacgg tggcggctta gggtctacac acaaacccga tgcgggccgc
     3181 tacctatgcc aacggccggg ggcttgggct accgtcggcg catggacttt gccgtgcgcg
     3241 ccccttcagc gacatcgccc atgggcgggc gccgcgcggt gctgcggggc ctggctgccg
     3301 cgacgctggc gtcgtgcgcg ctggtgcgcg cgcaggagcg gcctgaaaag cccaagctca
     3361 gcatcgccgt cggcggcaga acgacggtgt attacctgcc gctcacgatt gccgatcagc
     3421 tcggtttttt cgcccacgaa gggctggagg tcgatttgca ggaccacgcc ggcggcggcc
     3481 tggcgcttca gtcgctgctg caaggccgtg ccgatgtggc ggctggcggc ttcgaacaca
     3541 cgatcttgct gcgccaacgc ggcgtcaact gccgcgcttt cgcgctgctc gggcgcgcgc
     3601 cgcagttggt gtttggcgtc agcacgcgcg cgctgccgga gtttcgcgat ccgtcgcagc
     3661 tcaagggtcg ccgcgtgggc atctcggcgc cggattcgtc cacccactgg ttcgcgcggc
     3721 tggtgctggc gcgcgccggg ctggcggcca cggatgtcga gtacgtgggc gtcggcacct
     3781 cgacggcggc ggtgatggct ctgcgcgaag gccgcatcga cgccattgcc agcatcgacc
     3841 cggtcatcag catgctggag tttcgcggcg agatccgggt gctggccgac acgcgttcgc
     3901 cgcgcggcac gcaggagctg tatggcgggc ccatgccggg cggctgcatc tatgcgccgc
     3961 aggccttcgt ggtgcgctat ccgcaaaccg tgcaggccct caccaacgcc gcggtgcggg
     4021 cgctgaaatg gctgcagacc gccggcccga gcgacatcgt gcgcgccgtg cccgagtcct
     4081 acatgtacgg cgaccgcgcc atctacctgg cgtcgctgga gaaagcgcgc accgcgctgt
     4141 gccccgacgg cctgatgacc gaggaagcgg tggccacggc gcaccgcgtg gtggcgcagt
     4201 actacagcgg cagcgccccg gtgcgccccc aggcgccggg cgccacctac accaacgatt
     4261 tcgcgcgccg cgccaagctg aagtttcagg tggcttgagg gccgcggcca cgcctggacg
     4321 cgggcgccac agtcagcgct tgttcttgcc gctggccttg ggttcggcct tggccttggc
     4381 gccgcctttg ccagccgact tgcccgggct gtccttgggc gagggctttg ccgacttgct
     4441 ggccggcttg gcatcggcgc ggcgatcgtt cttgcccgcg cgctggtttt tcgccttgcc
     4501 cttgtcggcg ccgctggcct ttgcccgctc ggcgcgcgat tcagacaagt gcgaggcccg
     4561 cacggcgccg cgcgtggcgg tctggctgcg ctcgcgccgc gccgctgcct cgaccccatc
     4621 gcgtgcagcg gcggccgcgg cgcgcaccgg caggtacagc accacggcct ggccgcgctt
     4681 caagcccgcg cgggcgttgg tgtggttcca gccggccacg gtggcggccg gcaggtcgta
     4741 gcgggccgcc acgctggcaa tcgaatcgcc cttgcgcgcg cgcaccgagg tgcggcgcag
     4801 cacgatctcg ggcgtgtagg ccagttgcgc gttgttcacc acgtggctgg ccaccgcttg
     4861 cgcagcgccg ttgctgcgcc gcaccaccac ggtcgagccg ctcttgatca ccatgccgcg
     4921 cgggatgttg ttcatctcgc ggaaatcgtc ctcgtccatg ccgaagcggc tggccacggc
     4981 gcgcgagggc agggtgtcgg tggcgaccca ggccgtccac gacgccagcg agccgggcgc
     5041 cgccttggcc aggttctgct tgaaggtgtc ggcgttgttc cacggcagca ggatctgcgg
     5101 cgtgccggcg gcaaagatca ccggcttgcg ctgcgacggg ttgagcgcct tgaagtcttc
     5161 cacccgcacc tcggccagcc gcgccgccac gtcgacgtcg atgtcctgcg tgatgtcgac
     5221 ggtgtcgaaa aacgggtggt tttcgatcag cggcagcacg gcgcccatgc gctgcgggtt
     5281 ggcgatgatg ttcttcaccg cctgcagctt gggcacgtag ttgcgcgttt cggccggcat
     5341 gttgatgtcg ttgtagccgg tgggcaggcc cgcggcctgg ttgcgctcga tcgcgcgctt
     5401 gacgttgccc tggccccagt tgtaggccgc cagcgccagg tgccagtcgc caaacatgtc
     5461 gtgcagcttc tgcagatagt cgagcgcagc gcgggtcgag ccgagcacgt tgcgccggtc
     5521 gtcgcgcagc atgttctgcg tcagctggaa gtcgcgcccg gtgccgggca tgaactgcca
     5581 catgccggcg gccttggcgc tgctgacggc ctgcgggttg aaggccgatt cgatgtacgg
     5641 cagcagcgcc aattcggtcg gcatgccgcg cagttccagc tcttcgacga tgtggaagat
     5701 gtacaggcgc gagcggtcga ccatgcgctc gatgtagtcg ggccgcgtgg tgtaccactg
     5761 ctcatggcgc tcgaccagct cgccctgcag gtcgggcatg gcaaagccgc gccgcacgcg
     5821 ctcccacagg tcggcgggtg cttcgagcgc ggcaacctgg ccgccacgca actcggacac
     5881 cgccacgtcc ggcatctcgc cagaacgcgg catgcggggc gcggtgcgtg cggtggatgt
     5941 ggcggccgaa gcgccgtctg acgcaccgtc tgcgccaccg ccgggcgcgg cacagccggc
     6001 caatgccacg ctgacgacca gcgccagccc ttgataccac ttcatttgaa ctcgttcttc
     6061 cattgacgaa gggcggcgaa caccgccact tcgtcggttg cttggggtgc gtgcgagcgc
     6121 accgactgga tcaccgcagg ctcgcggctg cgcagaaagg ggttgatcgc ccgctcggtg
     6181 ccgatcgacg acggcagcgt gggctggccg cccgcgcgca accgctggca atgctgctcg
     6241 tagtcggcca gcgcctggtt gcccggctcg accgcgcgcg caaagcgcag gttgccgagg
     6301 gtgtattcat gcgcgcagca cacgcgcgtg tcgccgggca gcgcggccag cgcatcgagc
     6361 gaggccagca tctgcgccgg cgtgccttcg aacaggcgcc cgcagccgcc tgaaaacagc
     6421 gtgtcgccgc aaaacagcag cggcgcgcca gccacatcgt cagcgaaata cgcgatgtgc
     6481 cctgccgtgt ggcccggcac gtcgatgacg cgaaagctga cgcccagggt ctgcaccacg
     6541 tcaccgccct gcaacggcgt gtacggctcg gggatgcgct cgcgcgccgg cccgaacacc
     6601 tgcgccccgg tttccgcgcg caattcggcc acgccgccgg tgtgatcggc gtggtggtgc
     6661 gtgactagaa tgctggcgag ccgcagggcc agccgttcca gcgccgcgcg caccggtgcc
     6721 gcgtcgcccg ggtcgaccac cagggcttgc tggccgtcgt gcaacaacca caggtagttg
     6781 tcgttgaagg cgggcagtgc gatcaattcc atgtgcggcg gattataaaa accgcagcag
     6841 gcgctgccgc ccgtgcaacc tatctttaca tcgcccgtgc cgccgccgac gcttcctttg
     6901 catgagtggt ttcagacccc gcccggccag tacgtgctgg cgtgggagcg ggcgcagttc
     6961 gacgccgcgc tggccgacgt ctttggctac cacgcgctgc agatcgggct ggccgacatc
     7021 gatgcgctgg cggccaaccg catgccgcac cgctggctgg ccatgacaag cgcgccgggc
     7081 gcgccggatg aagacccgac gcccgcgacc gtggccgcgg gcgacgaagc gccgccacca
     7141 ccacgcatcg ccctcgtcac cgaccccacg gcgctgccgt ttgccgaagc cagcctcgac
     7201 ctggtggtgc tgccgcacac gctggagctg agcgccgacc cgcacgccag cctgcgcgag
     7261 gtgcagcgcg tgctggtgca cgagggccgg gtggcgattg ccgggctcaa cccggccagc
     7321 ctgtggggcc tgcggcagta ccgcgcgcac ctgctgcgcg gcggcggcca gggccagctg
     7381 tacctgcccg acgcgggcga attcatcggc cactggcgcc tgcgcgactg gctgcggctg
     7441 ctgcaattcg agctggaaag cctctcgttc ggctgctatg cgccggcggt gcgcagcgcg
     7501 cgcaacctgg cgcgctttgg ctggatggac cgcgtgggcg cgcgctggtg gccgatcttc
     7561 ggtgccgcat acttcatggt cgccgtcaag cgcagcgccg gccccaagct ggtgggcgcg
     7621 gcgtggaaga aatcgccgat accggcgaac gctcctgttt caatagctgg ccgcgctgga
     7681 ctggcaagcg ctggcggcca aaaagaccat gaatcgcatt gagatgtaca ccgacggcgc
     7741 ctgcaagggc aaccccggcc ccggcggctg gggcgtgctg ctgcgcgcgc ccggccttga
     7801 aaaagaattg ttcggcggcg agccgctgac caccaacaac cgcatggaac tcacggccgt
     7861 gatcgaaggc ctgcaagccc tcaagcgccc gtgcgaggtc gacctgtacc tggacagcca
     7921 gtacgtgcgc cagggcatca ccgaatgggt ggccggctgg aaggcgaggg gctggcgcac
     7981 ctcgaccaag cagccggtga aaaatgtcga cctgtggcaa aagctcgatg cgctggtcgc
     8041 cgagggcggt caccgcatcc agtggcactg ggtgcgcggc cacgccgggc atgaaggcaa
     8101 cgagcgcgcc gacgcgctgg ccaaccgggg cgtgccgggg cggcgctgac ggcgccgctt
     8161 taaaggtcag cgcgtacagc aaggcgcgcc cggcgatgct acattttgaa aaggtcggcg
     8221 ccgccggggc gccacagtgg taccgatccg acccttgaag ctctcgacct ccctcatcgc
     8281 cgccgtggca ctggcgggcg ccatcggcgc ggcctggtgg tttcagcgcc cgtcccagag
     8341 caagccggcg accgacggcc cggcccgggt ggccagcgcg ccggccgccg gcggcacggc
     8401 cgcaggcccg gtggtggtcg aatcggcgct ggtgcgcgtg cggcccttga gcgaagacgt
     8461 cgaagccgtc ggctcgctgc gctcgcgcca gggcaccgtg gtgcgggccg aagtcggcgg
     8521 ccgggtcacg cagatcaact tccgcgacgg ccagcgcatt cgccgtggcc agctgctggt
     8581 gcagctggac gaccgcctgc agcgcgccca ggtgcagcag gcgcaggccg aattgtcgat
     8641 tgccgagacc aaccaccgcc gcaacagcga gctggtgtcc aagggcttca tcagccgccg
     8701 tggcgt
//
LOCUS       DAOMVO010000082         8614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2116_length_8614_cov_15.293375, whole genome shotgun sequence.
ACCESSION   DAOMVO010000082 DAOMVO010000000
VERSION     DAOMVO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2116_length_8614_cov_15.293375"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..293
                     /locus_tag="PKV30_05720"
                     /pseudo
     CDS             69..293
                     /locus_tag="PKV30_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
     gene            complement(372..1799)
                     /gene="ffh"
                     /locus_tag="PKV30_05725"
     CDS             complement(372..1799)
                     /gene="ffh"
                     /locus_tag="PKV30_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRL29750.1"
                     /translation="MATALTDKLSGLVKKISGQARITESNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKAMGQEVLGSLKPGQALVGIVNRELAATMGEGVADLDLAAQPPAV
                     ILMSGLQGSGKTTTTAKLARHLIQKRKKKVLTVSGDVYRPAAIEQLKTVTAQAGADWF
                     PSTPDQKPLDIARAALDHAKKHFYDVLIVDTAGRLAIDEALMAEIKTLHGALKPVETL
                     FVVDAMQGQDAANTAKAFKEALPLTGIVLTKMDGDSRGGAALSVRQVTGAPIKFAGVS
                     EKIDGLEVFDAERHAGRILGMGDVVALVEQVTANVDIEAAKKLADKVKSGSGFDLQDF
                     LGQLQQMKQMGGLGSLMDKLPSQIAGKATDADLARAEKDIKRKEGIINSMTPQERRKP
                     ELLKATRKRRIAAGAGVHVQEVNRLLKEFEQMQGMMKQFKGGAMAKLMRKMGGGKGLG
                     GMLGGGGMPPLPPGFGGPGGPKRPF"
     gene            1963..2772
                     /gene="ccsA"
                     /locus_tag="PKV30_05730"
     CDS             1963..2772
                     /gene="ccsA"
                     /locus_tag="PKV30_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRL29751.1"
                     /translation="MILASASPVGLLLGAAAALAYAVPAVAARAIGAVTARRVLLLAW
                     LLHGLLLAWNLFGGAPRFGFAPALSITVWLVLTAYVVESQLYPQMRARWALAGFGALT
                     IVLALLFPGAPLPATSSPLLPLHWALGIAAYGLFAAAVAHGWLMTRAEAQMRHASPVA
                     EGGVPLLTLERLMFRFVGAGFLLLTLTMLAGIFFGEHLYGAAHTGWRWDHKRIFTLLS
                     WLAFATLLVGRSLFGWRGRRAVHMLYIGAGLLLLGYAGSRFVLEVLLGRTA"
     gene            2769..2993
                     /locus_tag="PKV30_05735"
     CDS             2769..2993
                     /locus_tag="PKV30_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HRL29752.1"
                     /translation="MKLLIVLLVVLAGVWLWRRGRRLSAPRANRAAGAQALPMVRCQR
                     CGVHVPGNEVVTGREGAYCSAAHRRESEGA"
     gene            3072..4766
                     /locus_tag="PKV30_05740"
     CDS             3072..4766
                     /locus_tag="PKV30_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRL29753.1"
                     /translation="MAHVAGMVESDGATGFARLWTAFMSARILIGLAILGLHLSLHLL
                     GRPTSGWLLAWCGAYVVLAAAVRLLGKPSLPGRPFDPQWLPTVGVDLVFFAVLQLQPA
                     TQPINYTPLLALPVLMAAIMGSRPLALGTAALVTLMLLGHAAWTMGDAAVASAADMGQ
                     AGLTGAGLLLLAFLTNQMAARLAREESIARGNRIEAQVQALVNNLVVESLPDGVLVVD
                     SNYLVRTANPAAHVMLGSDEEVTPHVFSLRDNPAWLQLMHIAQLTFADSPVDAADVIL
                     HHEDRMTSRLQVRTQRTPPIGDGGISLCVMFLQDLREAEARLRTEKLAAMGRMSAAVA
                     HEIRNPLAAISQANALLDEDLVQPAQRRLTTMVRQNAERLQHIVEDVLDIARAQGQAD
                     AAEMHTLALDAETAAFCAEWVQQHGATPRVRVALNTPEVQVQFAREHLRRILVNLLDN
                     AARYASERAGAIQVATHAVRHGPAVLMVWSDGAAMEPAVRRHLFEPFFSSESRSSGLG
                     LFICRELCERHGASVGYERTSREQNGQIVEGNEFFVSFRRAHSGHSTFNLEDTQFP"
     gene            4763..6301
                     /locus_tag="PKV30_05745"
     CDS             4763..6301
                     /locus_tag="PKV30_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRL29754.1"
                     /translation="MNSPETVPGAARVLVVDDEPDLRTLYELTLLREGHHVDAAGSLA
                     EARERLASGAFDVVITDMRLPDGLGLELLRDLATTQRKERCIMITAYGSAENAVEALK
                     CGAFDYLTKPVDLKQFRGVVASALQAPERAAAAPRATASPAAPAASAPIADATPAAAS
                     PAADPVAQGALARLVGDSAAMRAVKHRIAKVARSMAPVLVHGESGTGKELVAGAVHAC
                     SHRSSGPFVPVNCGAIPETLLEAEFFGARKGSYTGAAQDREGFFQAARGGTLFLDEIG
                     DLPLAMQAKLLRAIQERRVRALGDTQEEAVDVRIVSATHKDLAAEVQAGRFRQDLFYR
                     LNVIEIVVPPLRERREDLPALCQALLARIAADSGAPVVSLSARQMAEIAAAPLDGNVR
                     ELENLLHRAVALGEAGDSGFGELPAPVHQAPAAINIKAPDAVPPTAEPDLAAEPTASL
                     PPNLESWLDAQERAVLVRALGETGFNRTAAAARLGLNLRQMRYRMMRLGITAPSDDAG
                     DDAG"
     gene            6291..6884
                     /gene="ampD"
                     /locus_tag="PKV30_05750"
     CDS             6291..6884
                     /gene="ampD"
                     /locus_tag="PKV30_05750"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HRL29755.1"
                     /translation="MPADTTSQDAWRGGWWRGAEPLPSPNFGPRPAGAPIDLIVVHSI
                     SLPPGEFEGDFVQALFTNRLDWDVHPYFQQIRGLEVSAHFYVRRDGAAWQFVSCDDRA
                     WHAGASSFCGRDNCNDYSVGIELEGLEGGHFEPAQYATLARLCTALAAHYPIEAVAGH
                     EHIAPGRKQDPGPGFDWRRLQQFLSWPDQRFPRGTLA"
     gene            7062..>8614
                     /locus_tag="PKV30_05755"
     CDS             7062..>8614
                     /locus_tag="PKV30_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HRL29756.1"
                     /translation="MQTARGAAPALSTPTRFPATPAAPAATAAPHTLTHYQIMRRNGA
                     VVAFEPSKIAVAMMKAFLAVHGTQGAASASVREVVDGLTQAVVRALMRSRPGGGTFHI
                     EDVQDQVELGLMRGGHHEVARAYVLYRERRAQERAQQQAEVATPASTLRVLQKGELVA
                     LDQQALEALFSQACANLDADVKPEPILAETLRNLYDGVPVEEVYKAAILAARTLIEKE
                     PDYTYATARLLLHTIAAEVLGETVGLPELAARYADYFPQFIKKGVDNELLDERLQQFN
                     LARLGAALKPERDLKFDYLGLQTLYDRYFLHVRKTRIELPQAFFMRVAMGLALNEIDR
                     EARAIEFYEVLSSFDFMSSTPTLFNSGTLRSQLSSCYLTTVPDDLDGIYESIKENALL
                     SKFAGGLGNDWTRVRALGSHIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVCTY
                     LETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMRRVMEKGTWTLFSPSDVPD
                     LHDKFGADF"
BASE COUNT         1299 a         2949 c         2923 g         1443 t
ORIGIN      
        1 tttgctctgt tgttgatagc tgcccgcgct ggatatgcgg gcaccggcgg cctaagactc
       61 cttgaaacaa gaccgccggc ggcgtgctgc tgaacttcga ggtggccgat gtcgatgccg
      121 agtacgcccg cgcgcagcag acgggcctca agatcgtgct gccgctgcga gacgaggatt
      181 tcggccagcg gcacttcatc gcgcaagggc cggagggcgt gctgatcgac gtcatcacgc
      241 ccatcgagcc cagcgcggcg ttcaaggcgc tgtacctgga aggcgctggg taatcctgaa
      301 ttgatagctg ctcgcgcttg tctgtcaagc gctggcggcc tatttctttc aaagagcggc
      361 ggcgcgccgc gtcaaaacgg ccgcttcggc ccgcccggcc cgccaaagcc cggcggcagc
      421 ggcggcatgc caccgccgcc cagcatgccg cccaggccct tgccgccgcc catcttgcgc
      481 atcagcttgg ccatggcgcc gcctttgaac tgcttcatca tgccctgcat ctgctcgaac
      541 tccttcagca ggcggttcac ttcctgaacg tgcacaccgg cgccggcggc gatgcggcgc
      601 ttgcgcgtgg ctttcagcaa ctcgggttta cggcgctctt gcggcgtcat gctgttgatg
      661 atgccttcct tgcgcttgat gtccttctcg gcgcgcgcca ggtcggcgtc ggtcgccttg
      721 ccggcgatct ggctgggcag cttgtccatc aggctgccca ggccgcccat ctgcttcatt
      781 tgttgcagtt ggccgaggaa gtcctgcaaa tcaaagccgc tgccgctctt gaccttgtcg
      841 gccagcttct tggccgcctc gatgtcgacg ttggccgtga cctgctcgac cagcgccacc
      901 acgtcgccca tgcccagaat gcggccggcg tggcgctcgg cgtcgaacac ttccaggccg
      961 tcgatctttt cgctgacacc ggcaaacttg atcggcgcac cggtgacttg acgcacagac
     1021 agcgccgcgc caccgcgcga atcgccgtcc atcttggtca acacgatgcc ggtcagcggc
     1081 agcgcttcct tgaacgcctt ggcggtgttg gcggcgtcct gaccttgcat ggcgtcgacg
     1141 acgaacagcg tttcgacggg cttgagcgcg ccatgcagcg tcttgatctc ggccatcaac
     1201 gcctcgtcaa tcgccagccg gccggcggtg tcgacgatca gcacgtcgta gaagtgcttt
     1261 ttggcgtggt cgagcgcggc gcgggcgatg tcgagcggtt tctgatcggg cgtggagggg
     1321 aaccagtcgg ccccggcctg cgcggtgacg gtcttgagct gctcgatggc ggcggggcgg
     1381 tacacgtcgc ctgacaccgt gagcaccttc ttcttgcgct tctggatcag gtggcgcgcc
     1441 aattttgcag tggtagtggt cttgcccgag ccttgcaggc cgctcatcaa aatcaccgcc
     1501 ggcggctgcg ctgccagatc caggtcggcc acgccctcgc ccatggtcgc cgccagttcg
     1561 cggttgacga tgccgaccag cgcctggccg ggcttgaggc tgcccagcac ctcctgaccc
     1621 atggccttgt ccttgacgcg ggcgatgaag tcgcgcacca cgggcagcgc cacgtcggct
     1681 tcaagcagcg ccatgcgcac ctcgcgcagc atgtcctgca cgttgctttc ggtgatgcgg
     1741 gcctggccgc tgatcttctt gaccaggcca gagagtttgt cggtgagggc ggtggccatg
     1801 tcggtagtcc agcgcgaaca agccgcgcaa ctcatgaaat cagaaggccg cggaacaggc
     1861 cattcgggtg gccgccgcgg agcgggcttg gcccggccgc tggcggcgtc ccccgtcagg
     1921 gggaaaggcg cgtgagcgcc tcaggggggt aaactttctc ccatgattct agccagcgcg
     1981 tctcctgtcg gcctgctgct cggcgccgcg gccgcgctgg cctacgcggt gccggcggtg
     2041 gcagcgcgcg ccatcggcgc cgtgacggcg cggcgcgtgc tgttgctggc ttggctgctg
     2101 cacgggctgc tgctggcctg gaacctgttt ggcggcgcgc cgcgcttcgg ctttgcgccg
     2161 gcactgtcga tcaccgtgtg gctggtgctg acggcctacg tggtcgagag ccagctgtac
     2221 ccgcaaatgc gcgcgcgctg ggcgctggcc ggctttggcg cgctgaccat cgtgctggcg
     2281 ctgctgtttc cgggtgcgcc gctgcccgcc acttcgtcgc cgctgcttcc gctgcactgg
     2341 gcgctcggca tcgccgccta cggcctgttt gccgccgccg tggcgcatgg ctggttgatg
     2401 acgcgcgccg aggcgcagat gcgccacgcc tcgccggtgg ccgagggcgg cgtgccgctg
     2461 ctcacgctgg agcggttgat gttccgcttc gtcggtgccg gcttcttgct gctgacgctg
     2521 accatgctgg ccggcatctt ctttggcgag catttgtacg gcgccgcgca caccggctgg
     2581 cgctgggacc acaagcgcat cttcacgctg ctgtcgtggc tggcctttgc caccttgctg
     2641 gtcggtcgct cgctgttcgg atggcgcggg cgccgcgcgg tgcacatgct gtacatcggc
     2701 gccggcctgc tgctgctggg ctacgccggc tcgcgctttg tgctggaagt gttgctgggg
     2761 cgcacggcgt gaaactgctc atcgtgctgc tggtggtgct ggcgggcgtg tggctgtggc
     2821 ggcgcgggcg ccggctgtcg gcaccccgcg cgaaccgcgc cgctggcgcg caagcgttgc
     2881 ccatggtgcg ctgccagcgc tgcggcgtgc acgtgccggg caacgaggtg gtgacgggcc
     2941 gcgaaggcgc ctactgcagc gccgcccatc ggcgcgaatc cgagggcgct tagatggcgc
     3001 cgcccagccc gcgcgagcgg cgcacgcgct tcgtcgcatc gcaggcgccg ggttcttcct
     3061 tgccgccatc catggcccac gtggccggca tggtcgagtc cgacggcgcc acgggctttg
     3121 cgcgcctgtg gacggccttc atgtcggccc ggattctcat cgggctggcg attctgggcc
     3181 tgcacctaag cctgcacctg ctgggccgcc ccacctccgg ctggctgctg gcctggtgcg
     3241 gcgcctatgt ggtgctggcc gcggcggtgc gcctgctggg taaacccagc ctgccggggc
     3301 ggccgttcga tccgcagtgg ctgccgacgg tgggcgtcga tctggtgttt ttcgccgtgc
     3361 tgcagctgca gccggccacg caaccgatca actacacgcc cttgctggcg ctgccggtgt
     3421 tgatggcggc catcatgggc tctcgcccgc tggcgctggg cacggcagcg ctggtcacgc
     3481 tgatgctgct cggccacgcg gcgtggacca tgggcgatgc cgccgtcgcc agcgcggcag
     3541 acatggggca ggcggggctg accggggccg gtttgttgct gctggccttt ctcaccaacc
     3601 agatggccgc gcggctggcg cgcgaggaaa gcattgcgcg cggcaaccgc atcgaggcgc
     3661 aggtgcaggc gctggtcaac aacctggtgg tcgaatcgct gccggatggc gtgctggtgg
     3721 tcgattcgaa ctacctggtg cgcaccgcca acccggcggc gcacgtcatg ctggggtcgg
     3781 acgaggaggt gacgccccac gtgttcagcc tgcgcgacaa cccggcctgg ctgcagctga
     3841 tgcacatcgc gcagctgacg tttgccgact cgccggtcga tgcggcggac gtcatcctgc
     3901 accacgaaga ccgcatgacc agccggctgc aggtgcgcac gcagcgcacg ccgcccatcg
     3961 gcgatggcgg catcagcctg tgcgtgatgt ttttgcagga tttgcgcgag gccgaagcgc
     4021 gcctgcgcac cgaaaaattg gccgccatgg gccgcatgtc ggccgccgtg gcgcatgaaa
     4081 tccgcaaccc gctggccgcc atcagccagg ccaatgcgct gctcgacgaa gacctggtac
     4141 agccagccca gcggcgcctg accaccatgg tgcggcagaa cgccgagcgg ctgcagcaca
     4201 ttgtcgaaga cgtgctcgac atcgcccgcg cgcaaggcca ggccgatgcg gccgagatgc
     4261 acacgctggc gctcgatgcc gaaaccgccg ccttttgcgc cgaatgggtg cagcagcacg
     4321 gcgccacccc gcgcgtgcgg gtggcgctga acacgccaga ggtccaggtg cagttcgcgc
     4381 gcgaacacct gcgccgcatt ctggtcaatc tgctcgacaa cgcggcgcgc tacgcctcgg
     4441 agcgcgccgg cgcgatccag gtcgcgacgc acgccgtgcg ccatggcccg gccgtgctga
     4501 tggtgtggag cgatggcgcg gccatggagc cggcagtgcg gcgccacctg ttcgagccct
     4561 ttttctcgtc cgagagccgc tcgtcgggcc tgggcctgtt catctgccgc gaactgtgcg
     4621 agcgccatgg cgccagcgtg ggctacgagc gcacctcgcg tgagcaaaat gggcagatcg
     4681 tcgaaggcaa cgagttcttc gtcagttttc ggcgcgccca cagcggccat tcgacattca
     4741 atctggaaga cactcagttc ccatgaactc ccccgagact gtgcccggcg ccgcgcgcgt
     4801 gctggtggtg gacgacgagc ccgacctgcg cacgctgtat gagctgaccc tgctgcgcga
     4861 aggccaccac gtcgatgctg ctggttcgct ggccgaagcg cgcgagcggc tggccagcgg
     4921 cgccttcgac gtcgtcatca ccgacatgcg cctgcccgac ggcctggggc tggagctgct
     4981 gcgcgacctg gcgacgaccc agcgcaagga gcgctgcatc atgatcaccg cctacggctc
     5041 ggccgagaac gcggtcgagg cgttgaagtg tggcgccttc gactacctga ccaagccggt
     5101 cgatctgaag cagtttcgcg gcgtggtggc gtcggcgctg caggcgcccg agcgcgccgc
     5161 cgccgcgcca cgcgcgaccg cctcgcccgc agcacctgcc gcgtccgcac ccatcgccga
     5221 cgcgacgcct gcggcggcca gcccggccgc cgacccggtg gcacaaggcg cgctggcccg
     5281 cctggtcggc gattcggcgg cgatgcgcgc agtcaagcac cgcatcgcca aggtcgcgcg
     5341 cagcatggcg ccggtgctgg tgcatggcga atcgggcacc ggcaaggaac tggtggcggg
     5401 cgccgtgcac gcctgcagcc accgatcaag cggccccttc gtgcccgtga actgcggcgc
     5461 gatccccgag accctgctgg aagccgagtt tttcggcgcc cgcaaaggct cgtacaccgg
     5521 cgccgcgcaa gaccgtgaag gcttcttcca ggccgcgcgt ggcggcactt tgtttcttga
     5581 cgaaatcggc gatctgccac tggccatgca ggccaagctg ttgcgcgcga ttcaggagcg
     5641 gcgcgtgcgg gcgctcggcg acacgcagga agaagcggtg gacgtgcgca tcgtcagcgc
     5701 cacgcacaag gacctggcgg ccgaagtgca ggccgggcgt tttcgccagg acttgttcta
     5761 ccgtctcaac gtgatcgaga tcgtggtgcc gccgctgcgc gagcggcgcg aagacctgcc
     5821 cgcgctgtgc caggcgctgc tggcgcgcat cgcggcagat tcgggcgcgc cggtggtcag
     5881 cctgtcggcg cggcagatgg ccgagatcgc cgccgcgcca ctggatggca acgtgcgcga
     5941 attggagaac ttgctgcacc gcgccgtggc gctgggcgaa gccggcgatt caggctttgg
     6001 cgagctgcca gcgcccgtcc atcaagcgcc ggcagctatc aatataaaag cgcccgatgc
     6061 cgtcccacca accgcagagc ctgaccttgc ggccgagcca accgcatcgc tgccgcccaa
     6121 tcttgaaagc tggctcgatg cacaagagcg cgcggtgctg gtgcgtgcgc tgggcgagac
     6181 gggcttcaac cgcaccgccg ccgccgcgcg gctgggcctg aatctgcgcc agatgcgcta
     6241 ccgcatgatg cgcctgggca tcacggcacc cagcgacgat gcgggcgacg atgccggctg
     6301 acaccacgtc tcaagacgcg tggcggggcg gctggtggcg tggcgcagag ccgctgccct
     6361 cgcccaattt cggcccgcgt ccggccggag cgcccatcga cctgatcgtc gtgcactcga
     6421 tcagcctgcc gccgggcgag ttcgaaggcg atttcgtgca ggcgctgttc accaaccggc
     6481 tcgactggga cgtgcacccc tacttccagc agattcgcgg gctggaagtg tcggcgcatt
     6541 tctacgtgcg gcgcgacggc gcggcgtggc agttcgtcag ctgcgacgac cgcgcctggc
     6601 acgctggcgc atcgagcttt tgcggccgcg acaactgcaa cgattattcg gtgggcatcg
     6661 agctggaagg tctcgaaggc ggccacttcg agccagcgca gtacgccacg ctggcgcgcc
     6721 tgtgcacggc gctggcggcg cactacccca tcgaagcggt tgcagggcac gagcacatcg
     6781 cgcccgggcg caagcaagac ccgggaccgg gcttcgactg gcgccgcttg cagcaatttc
     6841 tcagctggcc cgaccagcgc tttcctcgcg gcacgctggc ctgacagctc aggcgccgcg
     6901 cgcgtggcaa tcgcgcaaaa cacaataggt agtggttgaa ctcaagcgga acactagata
     6961 tagtgtccct gttcgccatg cgccgacgcc ataaaaagca caaagagacc cccgtcgcgc
     7021 cgcgcaaccc ccgcacgaca acagcttacc gagaggacct catgcaaact gcccgcggcg
     7081 ccgcgcccgc cctcagcaca cccacgcgct ttccggccac gcccgcggcc cccgctgcaa
     7141 cggccgcgcc gcacacgcta acgcactacc agatcatgcg gcgcaacggc gccgtggtgg
     7201 ccttcgagcc cagcaagatc gccgtcgcca tgatgaaggc ctttttggcc gtgcatggca
     7261 cgcagggcgc ggcatcggcc agcgtgcgcg aggtggtcga tggcctgacg caggccgtgg
     7321 tgcgggcgct tatgcgctcg cgtccgggcg gcggcacctt ccacatcgaa gacgtgcagg
     7381 accaggtcga actcggcctg atgcgcggcg gccaccacga ggtggcgcgc gcctacgtgc
     7441 tgtaccgcga acgccgcgcg caagagcgcg cgcagcagca ggccgaggtc gcgacgccgg
     7501 catcgaccct gcgtgtacta caaaaaggtg agctggtcgc gcttgaccag caagcgctgg
     7561 aggcactttt ttctcaagcc tgcgccaatc tggacgctga cgtcaagccc gagccgatcc
     7621 tggccgaaac gctgcgcaac ctgtacgacg gcgtgccagt ggaagaggtc tacaaggccg
     7681 ccatcctggc cgcgcgcacg ctgatcgaga aggagcccga ctacacctac gccaccgcgc
     7741 gcctgctgct gcacaccatc gcggccgagg tgctgggcga gaccgtgggc ctgcccgagc
     7801 tggccgcgcg ctacgccgac tactttccgc agttcatcaa aaagggcgtc gacaacgagc
     7861 tgctcgacga gcgcctgcag cagttcaatc tggcgcggct cggcgcggcg ctcaagcccg
     7921 agcgcgacct gaagttcgac tacctgggcc tgcagacgct gtacgaccgc tacttcctgc
     7981 acgtgcgcaa gacgcgcatc gagctgccgc aagccttctt catgcgcgtg gccatgggcc
     8041 tggcgctgaa cgagatcgac cgcgaggcgc gcgccatcga gttctacgaa gtgctgtcgt
     8101 ccttcgattt catgagcagc acgcccacgc tgttcaacag cggcacgctg cgctcgcagt
     8161 tgtcgagctg ctacctgacc accgtgccgg acgacctgga cggcatctac gaatcgatca
     8221 aggagaacgc gctgctgtcg aagtttgccg gcggcctggg caacgactgg acgcgcgtgc
     8281 gcgcgctcgg cagccacatc aagggcacca atggcgaatc gcagggcgtg gtgccgtttt
     8341 tgaaagtggt caacgacacc gctgtggccg tcaaccaggg cggcaagcgc aagggcgcgg
     8401 tctgcaccta tctggaaacc tggcacctcg acatcgagga gttcctggag ctgcgcaaga
     8461 acaccggcga tgaccgccgg cgcacgcacg acatgaacac ggccaattgg attcccgacc
     8521 tgttcatgcg ccgggtgatg gaaaaaggca cgtggacgct gttttcgccc agcgacgtgc
     8581 ccgacctgca cgacaagttc ggcgctgact tcga
//
LOCUS       DAOMVO010000083         8600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2122_length_8600_cov_11.731539, whole genome shotgun sequence.
ACCESSION   DAOMVO010000083 DAOMVO010000000
VERSION     DAOMVO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2122_length_8600_cov_11.731539"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKV30_05760"
     CDS             <1..265
                     /locus_tag="PKV30_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL29757.1"
                     /translation="LGASVLTIRHLLSPVIAWLLARAFRLGAAETTILLTFAALPTAS
                     SCYVLAARMGYNGPYVAGLVTLSTVLGVASLPFALGVLGGFAP"
     gene            complement(340..1248)
                     /locus_tag="PKV30_05765"
     CDS             complement(340..1248)
                     /locus_tag="PKV30_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29758.1"
                     /translation="MAYTLRQVLPLLTKQELELFEASRAAAVKELTARQLTAKITRAR
                     ALRDKYRDTYRRQTVATQTGPGRARKQMGGENSRTEVKAEVMAEVLQRLEAQQAKVEA
                     RLAKTDARPAVRVPKAPGVPATKTNPKAAQRNRRTTGDAPASGDADQPMRGATGTKQR
                     QIDRRDRAAEKAGYDVVTARGAKSTRAAGAAARPAAKAPRGRKDTNAITGDALPVEKL
                     MRQVRGALQQQQGEPPVEGVPTPRRGIRSEVGGSPSDNAHGAVPTNMPALAQRLNPLK
                     AEPVNKKIHASARGRQKVETAKRDAR"
     gene            complement(1516..2565)
                     /gene="queG"
                     /locus_tag="PKV30_05770"
     CDS             complement(1516..2565)
                     /gene="queG"
                     /locus_tag="PKV30_05770"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRL29759.1"
                     /translation="MCGWARELGFARVGVADVDLATAEPGLLAWLNNGFHGGMDYMAA
                     HGLKRARPAELVPGTVSVITARMDYLPQGTPADWQPVEFARLDRPTEGIVSLYARGRD
                     YHKVLRARLQKLAERIAAEVGPFGHRAFTDSAPVLEAELATRSGQGWRGKHTLVLSRE
                     GGSMFFLGEIYVDLHLPRTEPTGAHCGQCTACIDVCPTRAIVAPHRLDARRCISYLTI
                     EHDGAIDEDLRPLIGNRIYGCDDCQLICPWNKYAQTSRLADFDARDGLAGSDLPTLFG
                     WNEEEFLRRTEGGPIRRIGHVRWLRNIAVALGNALAALPAGDTRRAPLQAALQARAEH
                     PSALVREHVQWALGR"
     gene            2650..3105
                     /gene="tsaE"
                     /locus_tag="PKV30_05775"
     CDS             2650..3105
                     /gene="tsaE"
                     /locus_tag="PKV30_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRL29760.1"
                     /translation="MTWRGEDETATFARRLAALPGLRDAFIALHGDLGAGKTTFVRHL
                     LRALGIDGRVKSPTYAVVEPHTTPAGLAVSHFDFYRFNDPREWEDAGFRDLFASPGLK
                     LAEWPEKAADLLPPADLSLHLQVQADETRAVRLSAHTPLGAQLLREVTA"
     gene            3102..4583
                     /locus_tag="PKV30_05780"
     CDS             3102..4583
                     /locus_tag="PKV30_05780"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL29761.1"
                     /translation="MTQPDPARRRVEPPNSARRRALIQSGALVLLLTTHHIARGAGIV
                     AVRIWPAEDYSRVTIESDTALKTTHRMIGTPPRLAVDIEGLELNPTLRELVAKVRADD
                     PNIAGIRVGQNAPGVVRLVLDLKQDIKPQVFTLTPVAAYQHRLVFDLYPAHPPDPLEQ
                     LIAERMKDLGGPAGAAPVASAQGMPKDVTDSLGDLIAKQIERANQITRRGDTTVARAP
                     SPPPRRPLPEQTPAAGEQSTSRPAPPRPAPARGSTDRLIIVALDPGHGGEDPGAIGPG
                     GTHEKQVVLQIALKLRDRINNATVGGSPMRAFLTRDGDFFVPLGTRVEKARRVGADLF
                     ISLHADAFMREEARGASVYALSDRGASSAAARWLANKENDADRIGGVNLGVKDSLVQR
                     AMMDMSTTAQIKDSLVLGDAVLREIGGVGRLHKRNVERAGFAVLKAPDIPSVLVETAF
                     ISNPEEERKLRSESYQNDLANALINGIQKYFAANPPLARNRQV"
     gene            complement(5027..5680)
                     /locus_tag="PKV30_05785"
     CDS             complement(5027..5680)
                     /locus_tag="PKV30_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRL29762.1"
                     /translation="MDIVMQLVDFILHVDRYLGAFVAQYGAWVYALLFLIVFVETGVV
                     VMPFLPGDSLLFVVGALAGAGLINYPLACAVLLAAAILGDQCNYSIGRYFGPKVFQWE
                     DSRFFNRRAFDKAHAFYERYGGVTVILARFMPFIRTFVPFVAGVAEMSRGKFSFFNVT
                     GALIWVLGLVTAGYVFGNLPWVQANLSKIIWALILVPGLIAIYGGWKAGRAERAAAS"
     gene            5804..6085
                     /locus_tag="PKV30_05790"
     CDS             5804..6085
                     /locus_tag="PKV30_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29763.1"
                     /translation="MATDIDKALNENPVPRGKRETTVDLADSGQRKDGDASPALPHES
                     DQQPGSQSPAADQDKTPEAKDAYRDAKLGRPDTSSAPLLQQPSPQDKSG"
     gene            6120..7916
                     /gene="mutL"
                     /locus_tag="PKV30_05795"
     CDS             6120..7916
                     /gene="mutL"
                     /locus_tag="PKV30_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRL29764.1"
                     /translation="MPPTPRRPIRELPDELISQIAAGEVVERPASVVRELLDNALDAG
                     ATQITLRLSAGGVRLISVEDDGAGIAPDEMATALKRHATSKIASLAELEAVGTMGFRG
                     EALAAINSIAEVALLSRAGGQSNAYSLDGRTGELKPAARAQGTTVEVKELFYSVPARR
                     KFLKTDATELAHCIEAVRRHALARPDVGFAIWHDGKLIEQWRAHPGDAGRAQRLGDVL
                     GEDFLADSVAVDHRAGPLHITGRAGLPDAARSRADQQFAYVNGRFVRDKVIVHAARAA
                     YEDVLHGQRQPVYVLHVAIDPARVDVNVHPTKIEVRFRDGREVHQAVRHAVENALAAP
                     RAALTGQLESVADTASPAFKPFEPPALTRQAQAAIKFAERGHPLGVEELRALWQPTPA
                     AVEAAPETETTHDWPLGRAIAQLHGVYILAENHAGLVLVDMHAAHERIVYERLKNQLD
                     GARITSQPLLIPATFAATPHEVATAESAAAVLQRLGLEITPFSPKTLAVRAVPTSLAA
                     GDAVDLARSVLAELSQHEAGTVIERAQHELLATMACHGAVRANRRLTHEEMNALLRQM
                     EATERSDQCNHGRPTWRQLSMKELDALFLRGR"
     gene            complement(8052..>8600)
                     /locus_tag="PKV30_05800"
     CDS             complement(8052..>8600)
                     /locus_tag="PKV30_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRL29765.1"
                     /translation="DSPVIIQSFETSNLKYLRTKTQVRLVQLIDGNDVNADGSMDFSL
                     PWGQAYDLTVAGDSRNYGALLTPAGLADIKKYADGIGPWKPYLIPTRLTIGADGKPVD
                     LNKDGAIDERDRTVMAPTAVLDNAHKAGLFVHPYTFRSEAKRLASDYKGDPKAEYKRF
                     YELGVDGVFADYPDHAKAARDD"
BASE COUNT         1394 a         3047 c         2854 g         1305 t
ORIGIN      
        1 gctgggcgca agcgtgctga ccatccggca cctgctgtcg cccgtcatcg cctggctgct
       61 ggcgcgcgcc ttccgcctgg gcgcggccga gaccaccatc ctgctcacct tcgccgcgct
      121 gcccacagca tcgagctgct acgtgctggc cgcgcgcatg ggctacaacg ggccgtacgt
      181 ggccgggttg gtcaccctgt cgaccgtgct gggcgtggcc agcctgccgt tcgcgctcgg
      241 cgtactgggc ggttttgctc cttgattaat agctgcttgc gcttgtcaga caagcgctgg
      301 cggccgattt gatcaataaa tacggccgcc cccagcgcat caacgcgcgt cgcgcttggc
      361 cgtctcgacc ttctggcggc cgcgtgccga ggcgtggatc ttcttgttca ccggctcggc
      421 cttcagcggg ttcaggcgct gcgccagcgc cggcatgttg gtcggcacgg cgccgtgcgc
      481 gttgtcgctg ggcgagccgc ccacctcgct gcggatgccg cggcgcggcg tcggcacgcc
      541 ttccaccggc ggctcgccct gttgctgctg aagcgcgccg cgcacctgcc gcatcagttt
      601 ttcgaccggg agcgcgtcgc cggtgatggc gttggtgtcc ttgcgtccgc gcggcgcctt
      661 ggcggccggg cgcgcggcgg cgcccgcagc gcgcgtggac ttggccccgc gcgcggtcac
      721 cacgtcgtag ccggccttct cggcggcgcg gtcgcggcgg tcgatttggc gttgcttggt
      781 gccggtggcg ccgcgcatgg gctggtcggc atcgccgctc gccggcgcat cgcccgtcgt
      841 gcggcggttg cgttgcgccg ccttggggtt ggtcttggtg gccggcacgc cgggcgcctt
      901 gggcacgcgc accgccgggc gagcgtcggt tttggccaag cgggcctcga ccttggcttg
      961 ctgggcttcc aggcgctgca gcacctcggc catgacttcg gccttgactt cggtgcggct
     1021 gttctcgccg cccatctgct tgcgcgcacg ccccgggccg gtctgcgtgg ccaccgtctg
     1081 gcggcgatag gtgtcgcggt acttgtctcg cagcgcgcgg gcgcgggtga tcttggcggt
     1141 caactggcgg gcggtcagct ctttcacggc cgccgcgcgg ctggcttcaa acagttcgag
     1201 ttcctgcttg gtcagcaggg gcagcacttg tctcagggtg taggccacgg ggtcgtctcc
     1261 ttggtcttgg gctggaccgg caaggcgctt gccggcgaag tgtttccgcc gcgaggccgc
     1321 aacggcacga aagcccatgc tatgcaaatg ccgagcgcgc ggctgtcgga caaggcgccc
     1381 ttggcgtgcc cgcctgcgcg cgcggcgttg cgcggccgac gttgacgagc ggtttagaat
     1441 gctatgaaaa tggcagcttg ccgcgctgat cgggcgcggg cccaccccct gaaaaacgct
     1501 agcaccgccg tgacttcacc gccccagcgc ccattgcacg tgctcgcgca ccagcgcgct
     1561 cgggtgctcg gcgcgggcct gcagcgcggc ctgcaacggc gcgcggcgcg tatcgcctgc
     1621 gggcagcgcg gccagcgcgt tgccgagggc cacggcgatg ttgcgcagcc agcgcacatg
     1681 gccaatgcgg cggatcgggc cgccttcggt gcggcgcaga aattcttcct cattccaacc
     1741 aaacagcgtg gggaggtcgc tgccggccag gccgtcgcgg gcgtcgaagt ccgccaagcg
     1801 gctggtctgc gcgtacttgt tccaggggca gatcagctgg cagtcgtcgc agccgtagat
     1861 gcggttgccg atcagcgggc gcaggtcttc atcaatggcg ccgtcgtgct cgatcgtcaa
     1921 gtacgagatg cagcgccgcg catccagccg gtgcggcgcc acgatggcgc gcgtggggca
     1981 aacgtcgatg caggcggtgc attgcccgca gtgcgcgccg gtcggttcgg tgcgcggcag
     2041 gtgcaaatca acgtagattt cgcccagaaa gaacatcgag ccgccttcgc gcgacagcac
     2101 cagcgtgtgc ttgccgcgcc agccctggcc actgcgagtg gccagctcgg cctcgagcac
     2161 cggcgccgaa tcggtgaagg cgcggtggcc gaacggcccc acttcggccg cgatgcgttc
     2221 ggccagcttc tgcagccgcg cgcgcagcac cttgtggtag tcgcggccgc gggcgtagag
     2281 cgagacgatg ccttcggtgg gccgatcaag ccgcgcgaat tcgaccggct gccaatcggc
     2341 tggcgtgccc tgcggcaagt agtccatgcg cgcggtgatc acgctcacgg tgcccggcac
     2401 cagctcggcc gggcgcgcgc gcttcaggcc gtgcgcggcc atgtagtcca tgccgccatg
     2461 aaagccgttg ttcaaccacg ccagcagccc gggctcggcc gtggccaggt cgacatcggc
     2521 cacgcccacg cgcgcaaagc ccagctcgcg cgcccagcca cacacctgcg tccacaaagc
     2581 aagggaatcg atttgagcgc cacatccagc cacgcccaaa ttgtagaaag caccccgccg
     2641 ccccggttga tgacctggcg cggcgaggac gaaaccgcca ccttcgcgcg ccgcctggcc
     2701 gccctgcccg gcctgcgcga tgccttcatc gccctgcacg gcgacctggg cgcgggcaag
     2761 acgacgttcg tgcgccatct gcttcgcgcg ctgggcatcg acggccgcgt gaagagcccg
     2821 acctacgccg tggtcgaacc gcacaccaca cccgctggcc tggccgtgtc gcatttcgac
     2881 ttctaccgct tcaacgaccc gcgcgaatgg gaagacgccg gttttcgcga tctgtttgct
     2941 tcgccgggcc tcaagctcgc cgagtggccc gagaaggcgg ccgacctgct gccgcccgcc
     3001 gatttgtcgc tgcacctgca ggtgcaggcc gatgagacgc gcgccgtgcg cctgtcagcc
     3061 cacacgccgc tgggcgccca actgctgcgc gaggtgacgg catgacgcaa cctgatcccg
     3121 cacgccgccg cgtagagccc cccaacagcg cacgccgccg cgcgctgatt caaagcggtg
     3181 cgctcgtgct gctgctcacc acgcaccaca tcgcgcgcgg cgccggcatc gtggccgtgc
     3241 gcatctggcc ggccgaggac tacagccgcg tcaccatcga atccgacact gcgctcaaga
     3301 ccacgcaccg catgatcggc acgccgccgc gcctggcggt cgacatcgag gggctcgaac
     3361 tgaaccccac gctgcgcgaa ttggtcgcca aggtgcgcgc cgacgacccc aacatcgccg
     3421 gcatccgcgt cggacagaac gcgcccggcg tggtgcggct ggtgctggac ctcaagcagg
     3481 acatcaagcc gcaggtgttc accctgacgc cagtggccgc ctatcagcac cggctggtgt
     3541 ttgaccttta tcccgcgcac ccgcccgatc cgctggagca actgattgcc gagcgcatga
     3601 aggacctggg cggcccggcc ggcgccgcgc ccgtggccag cgcgcaaggc atgcccaagg
     3661 acgtgaccga ttcgctgggc gatttgatcg ccaagcagat cgagcgcgcc aaccagatca
     3721 cgcggcgcgg cgacaccacc gtggcgcgcg cgcccagccc gccgccccgc cggccgttgc
     3781 ctgagcaaac gcccgccgcc ggcgagcaaa gcacctcgcg cccggcgccg ccccgccccg
     3841 cgcccgcgcg cggcagcacc gatcgcctga tcatcgtcgc gctcgacccc ggccacggcg
     3901 gcgaagaccc gggcgccatc ggccccggcg gcacgcacga gaagcaggtg gtgctgcaaa
     3961 tcgcgctcaa gctgcgcgac cgcatcaaca acgccacggt gggcggcagc ccgatgcgcg
     4021 cctttctcac gcgcgatggc gacttcttcg tgccgctggg cacgcgggtg gagaaggcgc
     4081 ggcgcgtggg cgccgacctg ttcatcagcc tgcacgccga tgccttcatg cgcgaggaag
     4141 cgcgcggcgc cagcgtgtac gcgctgtcgg accgcggcgc ctccagcgcg gcggcgcgct
     4201 ggctggccaa caaggaaaac gacgccgacc gcattggcgg cgtcaacctg ggcgtgaagg
     4261 attcgctggt gcagcgcgca atgatggaca tgagcaccac cgcgcaaatc aaggacagcc
     4321 tggtgctggg cgatgcagtg ctgcgcgaga ttggcggcgt tggccgcctg cacaagcgca
     4381 atgtcgagcg cgccggtttc gcggtgctga aggcgcccga cattcccagc gtgctggtcg
     4441 agaccgcctt catcagcaac cccgaggaag agcgcaagct gcgcagcgag agctaccaga
     4501 acgacctggc caacgcgctg atcaacggca tacaaaagta ctttgccgcc aatccaccgc
     4561 tggcgcgcaa tcgacaggtc tgacgtccac cccgtggcgc tgcgcgcctc ccctcaaggg
     4621 gcgtgcctgg cggccggtgc aagcccggcc gcgggcacct cgaatgcgcg gcggctgcgc
     4681 cactagcgct gagagttact atcaattcat gagcttctcg cgcttgttcc gcctacagca
     4741 acggcccatt tcgctcaaaa acatgggcgc cacgacagct cggcaagcgc gtggtcaact
     4801 cgccacgctt cgttgtcctg aagtgcgctt tgtcacacaa acgccgccga tcgctggtgg
     4861 acgacgcttc gggtcagcgc tgtctgacaa ccccgccgcc gcagcgcgcg ttgaatgaag
     4921 actggtgcca gcggcgcggg ccgcagcgcc aggtttcttg caagttctcc tccaaagctt
     4981 gagcgggcga ttccttcaac ggatcgcccg ttttttctta tggaggtcag gacgcggcgg
     5041 cgcgctccgc acgcccggct ttccaaccgc cgtagatggc gatcaagccc ggcaccagga
     5101 tcagcgccca gatgatcttg gataagttgg cctgcaccca cggcaggttg ccaaacacgt
     5161 agcccgccgt caccagcccc agcacccaga tcagcgcccc tgtgacgttg aagaagctga
     5221 acttgccgcg cgacatctcc gccacgccgg ccacgaaagg cacgaaggtg cggatgaagg
     5281 gcatgaagcg cgccaggatc accgtcacgc cgccatagcg ctcgtaaaac gcatgcgcct
     5341 tgtcgaacgc gcggcggttg aaaaagcgcg agtcttccca ctgaaacacc ttggggccga
     5401 agtagcgccc aattgagtaa ttgcactggt cgcccaaaat cgccgccgcc agcagcaccg
     5461 cgcaggccag cgggtagttg atgagcccgg cgccggccag cgcgccgacc acgaacagca
     5521 gcgaatcgcc gggcaagaag ggcatcacca cgacgcccgt ctcgacaaac acgatcaaga
     5581 acaaaagcgc atacacccac gcgccgtact gcgccacgaa ggcgcccagg tagcggtcga
     5641 cgtgcaggat gaagtcgacc agttgcatca cgatgtccat ggcgggcgat tatccgtgcg
     5701 ccgtgccgcg ccggcccacg gcgccagggc gctctgtccg acaggtggcg cccgcgcgcc
     5761 gcgccatact gaacgcacgc accgctggat gcaaggagcc cccatggcga ccgatatcga
     5821 caaagccctc aacgaaaacc ccgtgccgcg cggcaagcgc gaaaccacgg tcgatctggc
     5881 cgacagcggc cagcgcaagg acggcgatgc gtcgcctgcg ctgccgcacg aaagcgacca
     5941 gcaaccgggc agccagagcc ccgccgccga tcaggacaag acgcccgaag ccaaggacgc
     6001 gtaccgcgac gccaaactcg gccggcccga cacgtcatct gcgccgctgt tgcagcagcc
     6061 ctcgccgcag gacaagtcag gctgacaagg caacgccgcc ggccttccta gaatgcggca
     6121 tgcccccaac gccccgccgc ccgatccgcg aactgcccga tgaattgatc agccagatcg
     6181 ccgccggcga agtggtcgag cgcccggcgt cggtggtgcg cgagttgctt gacaacgcgc
     6241 tggatgccgg ggccacgcag atcacgctgc gcctgtcggc cggcggcgtg cgcctgatca
     6301 gtgtggagga cgatggcgcc ggcatcgcgc ccgatgagat ggccaccgcg ctcaagcgcc
     6361 acgccaccag caagatcgcc agcttggccg agctggaagc ggtgggcacc atgggttttc
     6421 gcggcgaggc gctggctgcc atcaattcga tagctgaagt cgcactgctg tcgcgcgctg
     6481 gcggccaatc caatgcttat tctttggacg gccgtacggg cgagctcaag cccgcggcgc
     6541 gcgcgcaagg caccacggtg gaagtcaagg agctgtttta cagcgtgccg gcgcgccgca
     6601 agttcctgaa gaccgacgcc accgaactgg cccactgcat cgaggccgtg cgccgccacg
     6661 cgctggcgcg gcccgacgtc ggttttgcca tctggcacga cggcaagctg atcgagcaat
     6721 ggcgcgccca ccccggcgac gcaggccgcg cgcagcggtt gggcgatgtg ctgggcgagg
     6781 attttttggc cgactcggtg gccgtcgatc accgcgccgg gccgctgcac atcaccggcc
     6841 gcgccggcct gcccgacgcc gcccgctcgc gcgccgacca gcagtttgcc tacgtcaacg
     6901 gccgcttcgt gcgcgacaaa gtcatcgtgc acgccgcgcg cgcggcgtac gaagacgtgc
     6961 tgcacggcca gcgccagccc gtgtacgtgc tgcatgtggc catcgacccg gcgcgcgtgg
     7021 acgtgaacgt gcacccgacg aaaatagagg tgcgctttcg cgacggccgc gaggtgcatc
     7081 aagcggtgcg ccacgcggtc gagaacgcgc tggcggcacc gcgcgctgca ctgacggggc
     7141 agcttgaatc ggtcgccgat actgcatcac cagctttcaa gccttttgag ccgccagcgc
     7201 ttaccagaca agcgcaagca gctatcaaat ttgcagagcg cggacatccg ctgggcgttg
     7261 aagaattgcg cgcgctgtgg caacccacac ccgccgccgt cgaagccgcg cccgaaaccg
     7321 agacgacgca cgactggccg ctcggccgcg cgatcgcgca gctgcacggc gtctacatcc
     7381 tggccgaaaa ccacgccggc ctggtgctgg tcgacatgca cgcggcgcat gagcgcatcg
     7441 tctacgagcg gctgaaaaac cagctcgacg gcgcccgcat caccagccag ccgctgctga
     7501 tccccgccac ctttgccgcc acgccgcacg aagtggccac cgccgaaagc gcggccgccg
     7561 tgctgcagcg cctggggctg gagatcacgc ccttctcccc caagaccctg gccgtgcgcg
     7621 ccgtgcccac cagcctggcc gccggcgacg cggtggatct ggcgcgcagc gtgctggccg
     7681 aactgagcca gcacgaagcc ggcaccgtga tcgagcgcgc ccagcacgag ttgctggcca
     7741 ccatggcctg ccacggcgcc gtgcgcgcca accgccgcct gacgcacgaa gagatgaacg
     7801 ccctgctgcg ccagatggaa gccaccgaac gctcagacca gtgcaaccac ggccgcccga
     7861 cctggcgcca gctatcgatg aaggagctgg acgcgctgtt tctgcgcggg aggtgacatt
     7921 gggatcgcca ttccggcgaa agccggaatc cacacgaggt ttccgaccaa aacagcgcca
     7981 tttcagcggc aacggcggtc tggattccgg ctttcgccgg aatgacgggg agctcttgag
     8041 cacccgtcgc ttcagtcgtc ccgcgccgcc ttggcgtgat cgggatagtc ggcaaacacg
     8101 ccgtccacgc ccagctcgta aaagcgcttg tattcggcct tggggtcgcc cttgtagtcg
     8161 ctggccaggc gcttggcctc gctgcggaag gtgtaggggt gcacgaaaag gcccgccttg
     8221 tgcgcgttgt ccagcaccgc cgtgggcgcc atcacggtgc ggtcgcgctc gtcgatggcg
     8281 ccgtccttgt tcaagtccac cggcttgccg tcggcaccga tggtcaggcg cgtggggatc
     8341 agatagggct tccaggggcc gataccgtcg gcgtatttct tgatatcggc caggccggcg
     8401 ggcgtcagca gcgcgccgta gttgcgcgaa tcgccggcca cggtcaggtc gtacgcctgg
     8461 ccccagggca ggctgaaatc catgctgcca tcggcgttga cgtcgttgcc gtcgatcagt
     8521 tgcaccaggc gcacctgcgt cttggtgcgc aggtatttca ggttgctggt ctcgaacgac
     8581 tggatgatga ccggcgaatc
//
LOCUS       DAOMVO010000084         8539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2135_length_8539_cov_14.091938, whole genome shotgun sequence.
ACCESSION   DAOMVO010000084 DAOMVO010000000
VERSION     DAOMVO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_8539_cov_14.091938"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKV30_05805"
     CDS             <1..570
                     /locus_tag="PKV30_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL29766.1"
                     /translation="HNMVFSEESTVGAFLLRARREGLRDFGACMSPHSAWLILQGIET
                     LPLRMERHIANTEKVVRFLAEHPFVGRVGHPLLESHPSHALANKLLKHGAKGAGSVFS
                     FDIKGSREQGKKFIETLRIFSHLANVGDVRSLVIHPASTTHFRMSDEALAGAGIGPGT
                     IRLSIGLEDADDLMDDLKRALKSAEKAGA"
     gene            573..1379
                     /locus_tag="PKV30_05810"
     CDS             573..1379
                     /locus_tag="PKV30_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29767.1"
                     /translation="MKIQVNGAPLYAYTGGKPIDVAKPTVVFIHGVLNDHSVWILQSR
                     YFAHHGWNVLAIDLPGQGKSGGQPPESVEHAADTVIALLDALGVDQAALVGHSFGSLI
                     ALEAAARAPARVSHLALVGTAFPMPVSPALLENSVKAPEKAIHMVNVFSHSTLCPPPS
                     ALGPGTWLYGGSRALMRRMLASNAETNVFYTGFKACNDYASGEQAMAAVHCPTLFLVG
                     RHDQMTPPKAAKALAAHARLAKIVEVNAGHALMTEAPDEVLFALRDFLAE"
     gene            complement(1393..2709)
                     /locus_tag="PKV30_05815"
     CDS             complement(1393..2709)
                     /locus_tag="PKV30_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HRL29768.1"
                     /translation="MKLSGLPARAKTFVTDVTDFPVLPTAATLWERFQQDRLGLTASS
                     LTFTTTLALVPFFAVVLAIFTAFPIFGQLKGALEQWLIESLIPTSIARNVLDYLTQFA
                     TKANELGTVGLLVLLFTALSLVLTIDHTLNDIWRVSVKRPLGQRILVYWAALTLGPLV
                     MGASLAITSYVLTASRGWLPDLADGVNLALDTVQFVLMMLGLTLLYRFVPNTPVKWKH
                     ALAGGLFVAVCLALAKQMLGWYLSRIPTYSMIYGAFATVPILLLWIYLSWLIVLFGAV
                     IAAYLPSLLSGVARRGGGPGWEFQLATEVLQSLARVRDTATRGLTLPQLANWLKVDTL
                     QLSPVMGALQALDWVGELGTQTENEEPRYVLLFDPPRQALRPLVEKLLLKPDKALSGW
                     WENDLMSDMTVADILPAKSGAFQDEKGSGARQSSARSSHKNSKSTP"
     gene            2732..3127
                     /locus_tag="PKV30_05820"
     CDS             2732..3127
                     /locus_tag="PKV30_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HRL29769.1"
                     /translation="MPTPPAATPSAAVQATRAVAVCSLLALIALGLAWELWLAPLRPG
                     GSWLAIKVLPLLLPLPGLLKNRMYTYRWVALFVWLYFVEGVVRASGDRPPGNHLALLQ
                     IAVCLVLFAACTLHVRLRQRSAQRAAASP"
     gene            3204..4625
                     /locus_tag="PKV30_05825"
     CDS             3204..4625
                     /locus_tag="PKV30_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL29770.1"
                     /translation="MNPSIALLDALRQAVGAAHVLTDGELIAYEQDWRQRERGRALAV
                     VRPGTTDEVAAVVRACAGHGAAIVPQGGNTGLAVGSVPDASGTQVLLSLRRMDAVRVI
                     DAANLTLSVEAGCILQNLQGAAQDAGFLFPLSLAAEGSCTIGGNLATNAGGTQVLRYG
                     NARELCLGLEVVTANGDIWNGQSGLRKDNTGYDLRDLFIGSEGTLGVITAATMKLYPP
                     PAARLTAWAAVPSMQAAVDLLGLAQRFLSAGLTGFEVMNQFALSLVAKHFPQQRVPLW
                     QDAPWCVLLENSDHESETHAREQFERLLEAALEAGCATDAVVAESLSQAKALWHIRES
                     IPLAQAEEGLNIKHDISVPVSRIPAFVAETDELLARAIPGVRLVDFGHLGDGNLHYNV
                     QAPEGVNAAAFLREQEDGINMLVFDSVVRHGGSISAEHGIGSLKAAKLPHYKSPVAMQ
                     MMRAIKGALDPQGIMNPGRVLAR"
     gene            complement(4692..6389)
                     /locus_tag="PKV30_05830"
     CDS             complement(4692..6389)
                     /locus_tag="PKV30_05830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL29771.1"
                     /translation="MASFEENTPFALEPQALAATETVAINNPFAALGLAPELQAAVAD
                     LGFTEPTAVQQRAIPLALAAHEGEFNDLMVSSQTGSGKTAAFLLPVLHTLLGMQGEAA
                     AREKAAWDAKVAAALAAGEPAPKRPRRKNPLESRHFKPAVPGALVLCPTRELAQQVAA
                     DAIDLVKHCRGLRIATVVGGMPYQMQIAKLQNANLVVATPGRLLDLASGGQIKLDQVR
                     FLVMDEADRMLDLGFADALAEIHQLTAQRQQTMMFSATFAPRIQQLALRVMHEGGTRV
                     QRVQIDSPQERHANIKQELYWADSPEHKRRLLDHWLRDPSIDQAIVFACTQVECEQLS
                     VELQQTGFAAVSLHGALSQAVRNRRLSALRDGRVQILVATDVAARGIDVPTITHVINF
                     GLPMKAEDYTHRIGRTGRAGRDGLAVTLAEIRDRRRLADIEAYTQQHFRPLVIDGLEP
                     TKQFPPVTGPKEFGRGGKPGGRGPRGGGGGREGFGATRRGNARGPRQIEGKLGFGRRE
                     SSGGSYQKESFGGFDGAPRRASAGGFAGKPAASYSKPGAALRTVKAKPYSPPKGRAR"
     gene            complement(6804..7283)
                     /locus_tag="PKV30_05835"
     CDS             complement(6804..7283)
                     /locus_tag="PKV30_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRL29772.1"
                     /translation="MPHTPVRSKRSATPLAESFTHRLHTLQKLTDKVTQDAYLAATGL
                     PLGEGRCLTAVGAFAPLSVKDLARRANVDKAQASRAAQALVDKGLVEKSASSADARGV
                     ELRFTPEGQRLWQRLMTVVEERNQQIVGCLSATERAQFDRILTQLVEHARAVADEKS"
     gene            7365..7913
                     /locus_tag="PKV30_05840"
     CDS             7365..7913
                     /locus_tag="PKV30_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL29773.1"
                     /translation="MPIIDRIHHVAYRCKNAKQTVEWYQKYLDMNFILAIAENEVPST
                     KEPDPYMHIFLDAGNGNVLAFFELPTKAEMLSPADANTPAWTQHLALKVDSVDTMMKV
                     KAKMEADGIDVLGPTDHTIFKSIYFRDPSGHRLELAANTSTPAMDKALDEVKWDMLNE
                     WDRTKKAPEHARWMHDGSGKVK"
     gene            8086..>8539
                     /locus_tag="PKV30_05845"
     CDS             8086..>8539
                     /locus_tag="PKV30_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRL29774.1"
                     /translation="MHALNETHDPQLQSWVASANDGQTDFPIQNLPFAIFRRQGSKEA
                     FRGGVAIGDQIVDMAAAVKAGVFTGDALEAAQAAAEPTLNHLMGLGPQRLSHLRKQLS
                     KALQTGSAQEAALQACLVPQAQAEYALPARIGDYTDFYTSVHHATNIGK"
BASE COUNT         1465 a         2829 c         2837 g         1408 t
ORIGIN      
        1 cacaacatgg tgttcagcga ggaaagcacc gtcggcgcct ttttgctgcg cgcgcggcgc
       61 gaaggcctgc gcgactttgg cgcctgcatg agcccgcact cagcctggct gattttgcaa
      121 ggcatcgaaa cgctgccgct gcgcatggag cgccatattg ccaacaccga aaaagtggtg
      181 cgctttctgg ccgagcaccc gtttgtcggt cgtgtcggcc acccgctgct cgagtcgcac
      241 cccagccacg cgctggccaa caaactgctc aagcacggcg ccaagggtgc cggcagcgtg
      301 ttcagcttcg acatcaaggg ctcgcgcgag cagggcaaga agttcatcga gactttgaga
      361 atcttcagcc acctggccaa cgtgggtgac gtgcgcagcc tggtcatcca ccccgccagc
      421 accacgcact tccgcatgag cgacgaggcg ctggccggcg ccggcatcgg gccgggcacg
      481 atccggctgt cgatcgggct ggaagatgcg gacgatttga tggacgattt gaagcgggcg
      541 ctgaagtcgg ctgaaaaggc gggagcttga acatgaaaat ccaggtcaac ggcgcccccc
      601 tctacgccta caccggcggc aagcccatcg acgtcgccaa gcccacggtg gtcttcatcc
      661 acggcgtgct gaacgaccac agcgtgtgga ttttgcaaag ccgttacttt gcgcaccacg
      721 gctggaacgt gctggccatc gacctgccgg gccagggcaa gagcggcggt cagccgcccg
      781 agagcgtgga gcatgcggcc gacacggtga tcgcgctgct ggatgccctg ggcgtggatc
      841 aggccgcgct ggtgggccac agctttggct cgctgattgc gctggaagcc gccgcgcgcg
      901 cgccggcgcg ggtaagccat ctggcgttgg tgggcacggc cttccccatg ccggtgtcgc
      961 ccgcgctgct ggagaactcg gtcaaggcgc ccgagaaggc gatccacatg gtcaacgtgt
     1021 tttcgcacag cacgctgtgc ccgccgccgt cggccctggg gccgggcacc tggctgtacg
     1081 gcggctcgcg tgcgctgatg cggcgcatgc tcgccagcaa cgccgagacg aatgtgttct
     1141 acaccggctt caaggcctgc aacgactatg cgagcggcga gcaggcaatg gcggccgtgc
     1201 attgccccac gctgttcctg gttggccggc acgaccagat gacaccgccc aaggccgcca
     1261 aggcgctggc cgcgcacgcg cggctggcca aaatcgtcga ggtgaacgcc ggccacgcgc
     1321 tgatgaccga ggcgcccgac gaggtgctgt ttgcgctgcg cgactttctg gccgagtgac
     1381 ggggcgcgtc gttcaggggg ttgatttgct atttttatga gagcttctcg cgcttgattg
     1441 gcgtgcccca gagccctttt catcttgaaa agcgccgctc ttggcgggca ggatgtcggc
     1501 caccgtcatg tcggacatca ggtcgttctc ccaccagcct gacagcgcct tgtcgggttt
     1561 gagcagcagt ttctcgacca gcggccgcag cgcctgacgc ggcgggtcga acagcagcac
     1621 gtagcgcggc tcttcgtttt cggtctgcgt gcccagctca ccgacccagt ccagcgcttg
     1681 cagcgcgccc atcacgggcg acagctggag ggtgtcgacc ttcagccagt tggccagctg
     1741 cggcagcgtg aggccgcgcg tggcggtgtc gcgcacgcgg gccagcgact gcagcacttc
     1801 ggtcgccagc tgaaactccc accccgggcc gccgccgcgc cgcgccacgc cggagagcaa
     1861 gctgggcaga taggccgcga tgacggcacc aaacagcacg atcagccacg acaggtagat
     1921 ccacagcagc aaaatcggca ccgtggcaaa ggcgccgtag atcatcgaat aggtgggaat
     1981 gcgcgacagg taccagccca gcatctgctt ggccagcgcc aggcacacgg ccacgaacag
     2041 gccgccggcc agcgcgtgct tccacttcac cggcgtgttg ggcacaaagc ggtacagcag
     2101 cgtcagcccc agcatcatca gcacgaactg caccgtgtcc agcgccaggt tgacgccgtc
     2161 ggccaggtcg ggcagccaac cgcgcgaggc agtgagcacg tacgacgtga tggccaggct
     2221 ggcgcccatc accagcgggc ccagggtgag cgcggcccag tagaccaaga tgcgctggcc
     2281 cagcgggcgc ttgacgctga cgcgccagat gtcgttcagc gtgtggtcga tcgtcagcac
     2341 cagcgacagc gccgtgaaca gcagcaccag caggcccacg gtgcccaact cgttggcctt
     2401 ggtggcgaac tgcgtcaggt agtcgagcac gttgcgcgcg atgctggtgg gaatcaggct
     2461 ttcgatcagc cactgctcca gcgcgccctt gagctgccca aagatgggga aggcggtgaa
     2521 gatcgccagc accacggcga agaaaggcac cagcgccagc gtggtggtga aggtgagcga
     2581 gctggccgtc agccccaggc ggtcttgttg aaagcgctcc cacagcgtgg ccgccgtggg
     2641 cagcacggga aagtcggtca cgtcggtgac gaaagtcttg gcgcgcgctg gcaggccgct
     2701 cagtttcatg ctcgctatga tgccatcgcc catgccgaca ccacccgccg ccacgccttc
     2761 cgccgccgtt caggccaccc gcgccgtggc cgtgtgcagc ctgctggcgc tgatcgcgct
     2821 cggcctggcg tgggagctgt ggctggcgcc gctgcggccg ggcggctcgt ggctggctat
     2881 caaggtgctg ccgctgctgc tgccgctgcc gggcctgctg aaaaaccgca tgtacaccta
     2941 ccgctgggtg gcgctgttcg tgtggctgta cttcgttgag ggcgtggtgc gcgccagcgg
     3001 cgaccggcca cccggcaacc acttggcgct gctgcagatt gcggtgtgcc tggtcttgtt
     3061 cgccgcctgc acgctccatg tgcggctgcg ccagcgcagc gcgcagcgag cggcagccag
     3121 cccatgaatt caatggcttt caggccgcca gcgctcgccc atcgtgcgct gtcagctatt
     3181 ttattgatag taagcagctt gtcatgaacc cttccatcgc cctcctcgac gccttgcgcc
     3241 aagccgtggg cgcggcccat gtgttgaccg atggcgaact gatcgcctac gagcaggact
     3301 ggcgccagcg cgaacgcggc cgcgcactgg ccgtggtgcg gccgggcacc accgacgagg
     3361 tcgccgccgt ggtgcgcgcc tgcgccgggc acggcgccgc catcgtgccg cagggcggca
     3421 acacggggct ggcggtcggc tcggtgcccg atgccagcgg cacgcaggtg ctgctgagcc
     3481 tgcgccgcat ggacgccgtg cgcgtcatcg acgctgccaa cctgaccctc agcgtcgaag
     3541 ccggctgcat cctgcagaac ctgcaaggcg cggcgcagga tgcgggtttt ctcttcccct
     3601 tgagcctggc cgccgaaggc agttgcacca tcggcggcaa cctggccacc aacgccggcg
     3661 gcacgcaggt gctgcgctac ggcaatgcgc gcgagctgtg cctggggctg gaagtggtga
     3721 ccgcgaacgg cgacatctgg aacggccaga gcggcctgcg caaggacaac accggttacg
     3781 acctgcgcga tctgttcatc ggcagcgaag gcacgctggg cgtcatcacc gcggcgacta
     3841 tgaagctgta cccaccgccc gccgcgcggc tcacggcctg ggccgcggtg ccgtcgatgc
     3901 aggcggcggt cgatctgctg ggcctggcgc aacgattctt gagcgccggg ctgacgggct
     3961 tcgaggtgat gaaccagttt gctctgagtc tggtcgccaa gcacttcccg cagcaacgcg
     4021 tgccgctgtg gcaagacgcg ccgtggtgcg tgctgctgga aaactcggac cacgaaagcg
     4081 aaacccacgc gcgcgagcag ttcgagcgcc tgctcgaagc cgcgctcgaa gccggctgcg
     4141 cgaccgacgc cgtggtggcc gaaagcctca gccaggccaa ggcgctgtgg cacatccgcg
     4201 aaagcattcc gctggcgcag gctgaggaag ggctgaacat caagcacgac atcagcgtgc
     4261 ccgtgtcgcg catccccgcc ttcgtggccg agaccgatga gctgctggcg cgcgccatcc
     4321 ccggcgtgcg cctggtcgat ttcggtcatc tgggcgatgg caacctgcac tacaacgtgc
     4381 aggcgccgga aggcgtgaat gcggcggcgt tcttgcgcga gcaggaagat ggcatcaaca
     4441 tgctggtgtt cgattcagtc gtccgccacg gcggctcgat cagcgccgag cacggcatcg
     4501 gcagcctgaa agcggccaag ctgccgcact acaagtcgcc tgtggcgatg cagatgatgc
     4561 gcgccatcaa gggtgcgctg gatccgcagg ggatcatgaa tccagggcgg gtgctggccc
     4621 gctgatcgca ccaaaaaaaa ggccgcagcg cccagcgggc gtgcggcctt cacttggcgc
     4681 gcgcggcgcg atcagcgtgc gcggcccttg ggcggcgaat aaggcttggc cttgaccgtg
     4741 cgcagcgcag cgccaggctt gctgtagctg gccgccggct tgccggcaaa accgcccgcc
     4801 gaggcccgcc gaggtgcccc gtcgaagccg ccaaaactct ctttttgata gctgccgccg
     4861 ctggattcgc gacggccgaa acccaattta ccttcgatct ggcgcgggcc gcgcgcgttg
     4921 ccgcgacgcg tggcgccaaa gccctcacga ccaccgccac cgccgcgcgg accccggccg
     4981 cccggcttgc caccacggcc gaattccttc ggcccggtga ccggcgggaa ctgtttggtc
     5041 ggctccagcc cgtcgatgac gagcgggcgg aagtgctgct gcgtgtacgc ctcgatgtcg
     5101 gccagacggc gccggtcgcg gatttcggcg agcgtcacgg ccagaccatc gcgcccggcg
     5161 cggccggtgc ggccgatgcg gtgcgtgtag tcctcggcct tcatcggcag gccgaagttg
     5221 atcacgtgcg tgatggtggg cacgtcgatg ccgcgcgcgg cgacgtcggt ggcgaccaga
     5281 atctgcaccc ggccatcgcg caatgccgac aggcggcggt tgcgcaccgc ctggctcagg
     5341 gcgccgtgca acgacacggc ggcaaagccg gtttgctgga gttctacaga caactgctcg
     5401 cattccacct gcgtgcaggc aaacacgatg gcctggtcga tgctcgggtc gcgcagccag
     5461 tggtcgagca ggcggcgctt gtgctcgggg ctgtcggccc agtacagctc ttgcttgatg
     5521 ttggcgtggc gctcttgcgg gctgtcgatc tgcacccttt gcacgcgcgt gccgccttcg
     5581 tgcatcacgc gcagggccag ttgctggatg cgcggcgcaa aggtggcgct gaacatcatg
     5641 gtttgctggc gctgagcggt gagttggtgg atttcggcca gggcgtcggc aaagcccaag
     5701 tcgagcatgc ggtcggcctc gtccatgacg aggaagcgca cctgatccag cttgatctgc
     5761 ccgccgctgg ccaagtcgag caagcggccc ggcgtggcca ccaccagatt ggcgttttgc
     5821 agcttggcaa tctgcatctg gtacggcatg ccgccgacca cggtggcaat gcgcaggccg
     5881 cggcagtgct tgaccaggtc gatggcgtcg gccgccacct gctgggccag ctcgcgcgtg
     5941 gggcacagca ccaaagcgcc gggcacggcg ggtttgaagt ggcgcgattc cagcgggttc
     6001 ttgcggcgcg ggcgcttggg cgccggctcg cccgcggcca gcgcggcggc aaccttggca
     6061 tcccacgcgg ctttttcgcg tgcggccgct tcgccctgca tgcccagcag cgtatgcaga
     6121 acgggcagca ggaaggccgc ggtcttgccg ctgccggtct ggctgctgac catcaggtcg
     6181 ttgaattcgc cttcgtgcgc ggccagcgcc agcggaatgg cgcgttgctg cacggcggtg
     6241 ggctcggtaa agcccagatc ggcgacggcc gcctgcagct caggcgccaa gcccagcgcg
     6301 gcaaacgggt tgttgattgc tacggtttca gtagctgcca gcgcttgtgg ttcaagcgcg
     6361 aacggcgtat tttcctcaaa ggaggccata agatctctcg tgtttccctg cgcgcaacgc
     6421 caacgccttg tccatggcgt ggggcacgac gcgtcagatg gggcgcccat gaaggacgcc
     6481 cgggtttcat gaatcgacac ccaccatctg actgcgcgaa cgtttcacat gccgtggtga
     6541 acacggccca aacatccggg aaaagcaggg ctggctgtaa aaccaaaccg tgctgaagat
     6601 caaggccact gggcgagagc tgttgaatgg cccgtcgcgg tggccgcatg caagaaggaa
     6661 gatgcggctg tttactctcg cggcttgaga aaaactcttg ccacgggaac tgcgcattat
     6721 ctcagggatt gccgatgtgt gcaatgccgg tcatgacgca actattcaat tgatagctgc
     6781 tcgcgcttgc cccgcttgcg cgatcagctt ttttcgtcag caacagcgcg cgcgtgctcg
     6841 accagctgcg tcagaatgcg gtcgaactgc gcgcgctcgg tggcgctgag gcagccgacg
     6901 atctgctggt tgcgctcttc caccaccgtc atcaggcgct gccacaggcg ctgcccctcg
     6961 ggcgtgaagc gcaactcgac gccgcgcgcg tcggcgctgc tggcggattt ttcgaccagg
     7021 cccttgtcca ccagcgcttg cgcggcgcgg ctggcctgcg ccttgtccac gttggcgcgc
     7081 cgcgccaggt ccttgaccga cagcggcgca aacgcgccca ccgcggtcag gcagcgccct
     7141 tcgcccagcg gcaggccggt ggcggccagg taggcatcct gcgtcacctt gtcggtcagc
     7201 ttttgcagcg tgtgcagccg gtgcgtgaac gactcggcca gcggcgtggc cgagcgcttg
     7261 ctgcggactg gggtgtgggg catcgggctt cctttttcag gcaattggtt gcgcgcgcaa
     7321 ccaattcatc ggataatacg tccaccccaa cacggagaca agccatgccc atcatcgacc
     7381 gcattcacca cgtcgcctac cgttgcaaga acgccaagca aaccgtcgag tggtaccaga
     7441 agtacctcga catgaacttc attctggcga ttgccgagaa cgaggtgcct tccaccaagg
     7501 aacccgatcc gtacatgcac atcttcctgg atgccggcaa cggcaatgtg ctcgcctttt
     7561 tcgagctgcc gaccaaggcc gagatgctct cgcccgccga cgccaacacg ccggcctgga
     7621 cgcagcactt ggcgctcaag gtcgattcgg tcgacaccat gatgaaggtc aaggccaaga
     7681 tggaagccga cggcatcgac gtgctgggcc ccaccgacca caccatcttc aagtcgatct
     7741 acttccgcga tccttccggc caccggctgg agttggccgc caacacctcg acgcccgcga
     7801 tggacaaggc gctggacgag gtcaagtggg atatgctgaa cgagtgggat cgcaccaaga
     7861 aggcgcccga gcatgcgcgc tggatgcacg acgggtcggg caaggtgaag taagcccggc
     7921 aaggcgctgt gagagcggcc ttggccgcaa ttcagcgcat cgccgcccgt ggcgcctggt
     7981 cgcgggcaag cccgctccca caaacttccc ttgaagccct atgacgaatc cggtctggcc
     8041 gcttcgtcag gcggcttttc tcatggaatc ctgacgagaa aacgcatgca cgcactgaac
     8101 gaaacgcacg acccccaact gcaaagctgg gtcgcctccg ccaacgacgg ccagacggat
     8161 ttcccgattc agaacctgcc cttcgccatc ttccgccgcc aaggcagcaa ggaggctttc
     8221 cgcggcggcg tggcgattgg cgatcagatc gtggacatgg ccgctgccgt caaggcgggc
     8281 gtgttcaccg gcgacgcgct ggaggcggcg caggcagcgg ccgagcccac gctcaaccat
     8341 ctgatgggtt taggccccca gcgcttgtcc cacctgcgca agcagctatc aaaagcattg
     8401 caaacgggat cggcgcaaga agcggcgctg caagcctgcc tggtgccgca agcgcaggcc
     8461 gaatacgcgc taccggcccg catcggcgac tacaccgact tctacacctc ggtgcaccac
     8521 gccaccaaca tcggcaagc
//
LOCUS       DAOMVO010000085         8434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2169_length_8434_cov_17.308390, whole genome shotgun sequence.
ACCESSION   DAOMVO010000085 DAOMVO010000000
VERSION     DAOMVO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2169_length_8434_cov_17.308390"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PKV30_05850"
     CDS             complement(<1..1307)
                     /locus_tag="PKV30_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRL29775.1"
                     /translation="MTFHPGLPHPALSQLPALLLFAMHLGVAARALTRPNRTPASRAA
                     WVAVIMLLPLVGVVAYFLLGETSIGRGRAQRLSETLKRLPPPPAPAAHPPSSQASALF
                     DLGRSINGLAPVAGNRITPLGDPHAPPDQPMRDSNAAIDTLVVDIAAAREHVHISFYI
                     WLDDHHGGQMADAVCAAARRGVACRVMVDALGSRAFIRSPRWRQLQDAGVRVLATLDD
                     IPRLGHLAVGRLDLRNHRKVAVIDNRIAFCGSQNCADPEFRVAARFAPWVDILLRCEG
                     PVVRQAQHLFLSTWIAETGEQIESLVSAPPAPEVFAPGVVAQMFGTGPTTAGNAMSDM
                     FVATLYAARHELIITTPYFVPDEALLRALCAAPRRGVRTVLVLPTRNNSKLVGAACYS
                     TYAPLLEAGVEIYEYPLGLLHTKSITADRQIALVGSANMDRRSL"
     gene            complement(1624..2121)
                     /locus_tag="PKV30_05855"
     CDS             complement(1624..2121)
                     /locus_tag="PKV30_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29776.1"
                     /translation="MALLDVLQQIIGSATAPDPKQIDAVTREASRDELKSGVGEALRS
                     KETPQLAQIVADMFEKASPEQRAAILNSLTEKLGPGALAGVAGGVLAGHEGATTPAVT
                     ADKATQITPAQVRDVVTTARTADEPGVFDRVSDFYSQHPDLVKTLGAGALMIALARMK
                     QNLGR"
     gene            complement(2229..3104)
                     /gene="rarD"
                     /locus_tag="PKV30_05860"
     CDS             complement(2229..3104)
                     /gene="rarD"
                     /locus_tag="PKV30_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HRL29777.1"
                     /translation="MWASLLAYGLWGLMPLYFRLMAPAGAVEVVAHRVVWSFVLLAAL
                     LGARRQIGALWRRLDAAQLARFALAALLVSINWLTYVWAVTHGHTLDASLGYFLLPLF
                     IVALGVLLLGERLGRAEWIGVVLAALGVAWLAWASPRFPWVALVLAASFGIYSLVKKR
                     THLAPTEGMLLETALIAVPALAVLAWLGAQGELAFGHGARGTDLLLISTGLMTTVPLV
                     AFAYAAQRLDLATLGMLQYLSPTLQFLLGVFVLHEPMDARRLAGFAAVWAGLAVFSAG
                     KWARGRAVAPAAPSA"
     gene            complement(3139..3360)
                     /locus_tag="PKV30_05865"
     CDS             complement(3139..3360)
                     /locus_tag="PKV30_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRL29778.1"
                     /translation="MGIEWLRPAAFFGSIVYALIGALIFWLCFIVIDKLTPYDLWAEI
                     VEKQNRALGMVVAAMCLGISVIIAAAIHG"
     gene            complement(3391..4914)
                     /locus_tag="PKV30_05870"
     CDS             complement(3391..4914)
                     /locus_tag="PKV30_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HRL29779.1"
                     /translation="MSEPTAQRFYRAPCPACGAPVEFKSAQSTHAVCAYCQSTVVREG
                     EVLKRIGKMAELFDDHSLLQLGASGQIDGQGFTLVGRLQYAYGEGDERGTWTEWHALQ
                     TDGSSAYLSEDNGAYVYSRPVSSNGAVPPASELPVGHSVALGGQRYVVSSNQPVTLIA
                     AQGELPKLPPLGQPFTVVEVRSEGGDEASQRVLSFDYGSAPPAVTLGRGVVLADLNMS
                     GLKAESAQEGSGRQFACPNCGSPVTVKLAESKAITCPQCHTLIDLSKGIGGELRHAVQ
                     DEPVRPLIALGSVGQLNGIHWQVVGFQHRLGQSPDGDESFGWTEYLLYNAKRGFAFLV
                     DAEDGWSLVRPTTGAPKVTGNGAKAEYAGATYSRTWSYNAETSYVLGEFYWPVRRGQK
                     TFNRDYAVGERLLSLEQTGSEQTWSAGAKLDFNQVARAFKLQDKAALFRRQDAAPVSL
                     GDAGSTLIKIIVVMMLVLAGMWLLAAMFGASSSGSGSGYGRSSGGSFGGYSSGGGHK"
     gene            complement(5250..6302)
                     /locus_tag="PKV30_05875"
     CDS             complement(5250..6302)
                     /locus_tag="PKV30_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRL29780.1"
                     /translation="MALMDFIKKQFIDVLQWTEDSDGTLAWRYPMAGMEIQNGATLVV
                     RESQMALFVDEGKVADVFGPGTYKLTTQTLPVLTYLKNWDKLFESPFKSDVYFFSTRQ
                     QVDQKWGTPQPVTVRDAEFGAVRIRAFGNYAYRVVDPKKFHTEISGTREAYTVQDLDG
                     QLRGLIMQQISVAIAQSKIPFLDLAANQLLFAQALSQALNPAMEGYGLKLEAMTVQSI
                     SLPEELQKVLDQKIGMGMVGNDMGKFMQYQTAQAIPKMAEGVGAGGDAGGLAGAGLGM
                     GAGVAMGQILAQNMQSALQGTAPAAGAAAAAAPVGVKPEDVMATLEKLGELKSKGILT
                     DDEFNAKKAELLKKLV"
     gene            6441..6665
                     /locus_tag="PKV30_05880"
     CDS             6441..6665
                     /locus_tag="PKV30_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HRL29781.1"
                     /translation="MARFIRNNRRMLQKTATYYVMHITVAAAVAYAVTGDLMMAFALS
                     LLEPTVQAVAFFVHEKLWSRAASPAVAAAA"
     gene            6697..>8434
                     /locus_tag="PKV30_05885"
     CDS             6697..>8434
                     /locus_tag="PKV30_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRL29782.1"
                     /translation="MPQNDHKPTEEKRADLRRAALDYHEFPVPGKIAIAPTKQLVNQH
                     ELSLAYSPGVAAPCEEIVKDPAAAFKYTARGNLIGVITNGTAVLGLGDIGALAGKPVM
                     EGKGVLFKKFSGIDVFDIEINEKDPYKLVEIIAALEPTFGGINLEDIKAPECFIVERE
                     LRKRMKIPVFHDDQHGTAIVVGAAVVSGLKVVGKKIEDVKLVTSGAGAAALACVGLLV
                     KLGLRRENIWVTDIKGLVYDGRTELMDPDKAIYAQKTDKRLLAEVIDGADVFLGLSAG
                     GVLKGDMVARMAPRPLILALANPYPEITPEEVKAVRDDAVMATGRTDYPNQVNNVLCF
                     PYIFRGALDCGATTITDEMEIAAVHAIADLAQAEQSEVVARAYVGEKLAFGPEYLIPK
                     PFDPRLMMKIAPAVAEAAAASGVAQRPIADLDAYRDKLQTFVYASGTTMKPIIDAARN
                     ASRKRVAYAEGEEERVLRAAQIVVDDGIARPTLIGRPAIIAQRIEKFGLRLTEGVDYD
                     VVNVEHDDRYKGFWQTYHELTKRRGITVPIAKIEMRRRLSLIGAMLLRSGDVDGLICG
                     TWGTNQMHLTYID"
BASE COUNT         1404 a         2839 c         2867 g         1324 t
ORIGIN      
        1 aggctgcgcc ggtccatgtt ggccgagccg accagcgcga tctggcggtc ggcggtgatc
       61 gacttggtgt gcagcaggcc cagcgggtat tcgtagatct ccacgccggc ctccagcagg
      121 ggcgcgtagg tgctgtagca cgcggcgccg accagcttgg agttgttgcg cgtgggcagc
      181 accagcacgg tgcgcacgcc gcgccggggc gccgcgcaca gggcgcgcag cagcgcctcg
      241 tcgggcacga agtaaggcgt ggtgatgatc agctcatgcc gcgccgcgta cagcgtggcg
      301 acgaacatgt cggacatggc gttgccggcc gtggtcggcc cggtgccaaa catctgcgcc
      361 accacgccgg gcgcgaacac ctcgggcgcg ggcggggcgc tgaccagcga ttcaatttgc
      421 tcgccggtct cggcgatcca ggtgctcaag aacaggtgct gcgcctgccg caccaccggg
      481 ccttcgcagc gcagcaggat gtcgacccag ggcgcaaagc gcgcggccac gcggaattcg
      541 gggtcggcgc agttctggct gccgcaaaag gcgatgcggt tgtcgatgac ggcgaccttg
      601 cggtggttgc gcaagtcgag ccggccgacg gccaggtggc ccaggcgtgg aatgtcgtcc
      661 agcgtggcca gcacgcgcac gccggcgtcc tgcaactgcc gccagcgcgg cgagcggatg
      721 aaggcgcgcg agccgagcgc atcgaccatc acgcggcagg ccacgccgcg ccgggcggcg
      781 gcgcagacgg cgtcggccat ctggccgccg tggtggtcgt ccagccagat gtagaagctg
      841 atgtgcacgt gttcgcgcgc ggcggcgatg tcgaccacca gggtgtcgat ggcggcgttg
      901 gagtcgcgca tgggctgatc gggcggggcg tgcgggtcac ccagcggcgt gatgcggttg
      961 ccggcgacgg gcgccaagcc gttgatcgag cggccgaggt cgaacagcgc gctggcttgg
     1021 ctgctgggtg gatgcgcggc gggcgccggg ggcggcggca agcgtttcag ggtctcgctc
     1081 agccgctgcg cgcggccgcg tccgatgctg gtttcgccga gcagaaagta cgccaccacg
     1141 ccgaccagcg gcagcagcat gatgaccgcg acccaggcgg cgcgcgaggc cggcgtgcgg
     1201 ttgggccgcg tgagcgcgcg ggccgccacg cccagatgca tcgcaaacag cagcagcgcg
     1261 ggcagctggc tgagcgccgg gtgcggaaga ccggggtgga aggtcatgcg ggggccatgg
     1321 aacgctgata gttccgtgag gctaccgcaa gcgggcggtt cggcggaagc ccgcgccgcc
     1381 ttgcgcggga tgggcgcagt gcgccgaacg ctacattaaa cgcagctgct tgcgcttgcc
     1441 ggacaagcgc tggcgcccta tttttcgtat agacaccccc acaaacaata caacgccgtt
     1501 cgtcctgagc ctgtgaagga ctgcgtatga gggggaccgg cttcgacagg ctcagcccga
     1561 acggatgagg ggctgttgga ctatttgtca gccccagata taaatccaga gcgccggagc
     1621 cgttcagcgc cccaggttct gcttcatgcg cgccagcgcg atcatcagcg cgccggcgcc
     1681 cagcgtcttc accaggtcgg ggtgctgcga atagaagtcg ctcacgcggt cgaacacgcc
     1741 cggctcgtcg gcggtgcggg cggtggtcac cacgtcgcgc acttgcgcgg gggtgatttg
     1801 cgtggccttg tcggcggtca cggcgggcgt ggtggcgcct tcatggccgg ccagcacgcc
     1861 gccggccaca ccggccagcg cgccggggcc gagcttttcg gtcagcgagt tcaggatggc
     1921 ggcgcgctgc tcgggcgagg ctttttcgaa catgtcggcc acgatttgcg ccagctgtgg
     1981 cgtttctttg gagcgcagcg cttcgccgac gccgcttttc agctcgtcgc gcgaggcttc
     2041 gcgcgtgacg gcgtcgatct gcttggggtc gggcgcggtg gcgctgccaa tgatttgttg
     2101 caggacatcg agaagggcca tggtgatctc cgttcaggtg ggtgaatcaa tcggccatcg
     2161 tagccgcgcc gcatggcggc gcggcacgcg cggcgtcaac tgtcgaactt gtccgacacg
     2221 tgcggcgctc aggccgatgg cgccgcgggt gccacggccc gcccgcgcgc ccacttgccg
     2281 gcgctgaaca ccgcgagccc ggcccacacc gccgcaaagc cggccagccg ccgcgcatcc
     2341 atcggctcgt gcagcacaaa cacgcccagc aaaaactgca gcgtgggcga caggtactgc
     2401 agcatgccca gcgtggccag atccagccgc tgcgccgcat aggcaaaggc gaccagcggc
     2461 acggtggtca tcaggccggt gctgatcagc agcagatcgg tgccgcgcgc gccatggcca
     2521 aacgccagct cgccctgcgc gcccagccac gccagcacgg ccagcgcggg cacggcgatc
     2581 agcgccgttt ccagcagcat gccctcggtt ggcgccaggt gggtgcgctt cttcaccagg
     2641 ctgtagatgc cgaagctggc ggccagcacc agcgccaccc acggaaagcg cggcgaggcc
     2701 caggccagcc aggccacgcc cagcgccgcc agcaccacgc cgatccattc ggcgcggccc
     2761 aggcgctcgc ccagcagcag cacgcccagc gccacgatga acagcggcag cagaaagtag
     2821 cccaggctgg cgtccagcgt gtggccgtgc gtcacggccc agacgtaggt cagccagttg
     2881 atgctgacca gcagcgccgc cagcgcaaag cgcgccagtt gcgccgcgtc gagccgccgc
     2941 cacagcgcgc cgatctgccg ccgcgcgccc agcagggcgg ccagcagcac gaaggaccac
     3001 accacgcggt gcgccaccac ctcgaccgcg ccggccggcg ccatgaggcg aaagtacagc
     3061 ggcatcagcc cccacagccc ataggccagc agcgacgccc acacgccgcc gctcacagcg
     3121 cggcccccgc gcgggcgctc atccgtggat ggcggcggcg atgatgaccg agatgcccag
     3181 gcacatggcg gccaccacca tgccgagcgc gcggttctgc ttttcgacga tttcggccca
     3241 caggtcgtag ggcgtgagct tgtcgatgac gatgaagcac agccagaaga tcagcgcgcc
     3301 gatcagtgcg tagacgatgg agccgaagaa agcggccggc cgcagccatt caatgcccat
     3361 gagaacctcc atgcaggaaa tgggtgaaat tcacttgtgc cccccgcccg agctgtagcc
     3421 gccaaagctg ccgcccgacg agcggccgta gccgctgccc gagccgctgc tgctggcgcc
     3481 aaacatcgcc gccagcagcc acatgccggc cagcaccagc atcatcacca cgatgatctt
     3541 gatgagggtg ctgccggcgt cgcccaggct gaccggcgcc gcgtcctggc gccggaacag
     3601 cgccgccttg tcttggagct tgaaggcgcg cgccacctgg ttgaagtcga gcttggcgcc
     3661 ggccgaccag gtctgctcgc tgccggtctg ctccagcgac aggaggcgct cgcccacggc
     3721 gtagtcgcgg ttgaaggttt tctgcccgcg ccgcaccggc cagtagaact cgcccagcac
     3781 gtagctggtt tcggcgttgt agctccaggt gcggctgtag gtggcgccgg cgtattcggc
     3841 cttggcgccg ttgccggtga ccttgggcgc gccggtggtg gggcgcacca ggctccagcc
     3901 gtcttcggca tcgaccagaa aggcaaagcc gcgcttggcg ttgtagagca ggtattcggt
     3961 ccagccaaag ctctcgtcgc cgtcgggcga ttggcccagg cggtgctgaa acccgaccac
     4021 ctgccagtga atgccattca attgccccac gctgccgagt gcaatcagcg ggcgcaccgg
     4081 ctcgtcctgt acggcgtggc gcagctcgcc gccaatgccc ttgctcaggt cgatcaacgt
     4141 gtggcactgc gggcaggtga tggccttgga ttcggccagc ttcaccgtca ccgggctgcc
     4201 gcagttgggg caggcgaact ggcggccgct gccttcctgg gcggattcgg ccttcagccc
     4261 actcatgttc aggtcggcca gcaccacgcc gcgccccagc gtgacggcag gcggcgcgct
     4321 gccgtagtcg aacgacagca cgcgctggct ggcttcgtcg ccgccttcgc tgcgcacctc
     4381 gaccacagtg aacggctggc ccagcggtgg cagcttgggc aattcgccct gcgcggcgat
     4441 cagcgtcacc ggctggttgg acgacaccac gtagcgctgc ccgcccagcg ccaccgagtg
     4501 gccgaccggc agctcgctgg ccggcggcac ggcgccgttg ctgctgaccg ggcggctgta
     4561 aacgtaggcg ccgttgtcct cgctcaagta cgcgctgctg ccatcggttt gcagcgcgtg
     4621 ccattcggtc caggtgccgc gctcgtcacc ctcgccgtac gcgtattgca ggcggccgac
     4681 cagggtgaag ccctggccgt cgatctggcc gctggcgccc agctgcagca ggctgtggtc
     4741 gtcgaacagc tcggccatct tgccgatgcg cttgagcacc tcgccctcgc gcaccaccgt
     4801 gctctggcag taggcgcaga cggcgtgggt cgattgcgcg ctcttgaact cgaccggcgc
     4861 gccgcaggcg gggcagggcg cgcggtagaa gcgctgggcg gtgggctcgc tcatcgggtg
     4921 gcggctacgg ggcgcgagcc gttattcaag gaaaaagcgg ctccagcgct tatctggtaa
     4981 gcgcgaacag ctcttctttt aatagtaatt tggtcagcat tcaccgccct gatgcgcgcg
     5041 caccccatcc agccggcgcg cagggtgatt cgggtgggca ttggaaggaa aaaagggcgt
     5101 ttgcgctcgt ccatcaagcg caagcagcta tgaaaacaag agtatctggg tggtttgccg
     5161 tggaagcggc cttggccgcg atcagcgccg cagacgggcg gcgcgcccga tcgcgggcaa
     5221 gcccgctccc accaagaccg gggcgcatct caaaccagct ttttcagcag ctcagccttc
     5281 ttggcgttga actcgtcgtc ggtcaggatg cccttggact tgagttcgcc cagcttttcc
     5341 agcgtggcca tcacgtcctc gggcttcacg cccacgggcg cggcagccgc cgcagcgccc
     5401 gccgccggcg ccgtgccttg cagggccgat tgcatgttct gcgccaatat ctgccccatc
     5461 gccacgcccg cgcccatgcc gaggccagcg ccggccaggc cgcccgcgtc gccgccagcg
     5521 cccacgccct cggccatctt cggaatggcc tgcgcggtct ggtactgcat gaacttgccc
     5581 atgtcgttgc cgaccatgcc catgccgatc ttctggtcga gcactttctg caactcttcc
     5641 ggcaggctga tgctttgcac cgtcatggcc tcgagcttga ggccgtagcc ttccatcgcc
     5701 gggttcagcg cctggctgag cgcctgcgca aacagcagct gattagccgc caggtcgagg
     5761 aacggaatct tgctttgcgc aatcgccacg ctgatctgct gcatgatcag cccgcgcaac
     5821 tggccgtcca ggtcttgcac cgtgtacgcc tcgcgcgtgc cggagatttc ggtgtggaat
     5881 ttcttcgggt cgaccacccg gtaggcgtag ttgccaaagg cgcgaatgcg cacggcgcca
     5941 aactcggcgt cgcgcaccgt caccggctgc ggtgtgcccc atttctggtc gacctgctgg
     6001 cgcgtgctga agaaatagac gtcgctcttg aagggcgact cgaacagctt gtcccagttc
     6061 ttcaagtacg tcagcaccgg cagcgtctgc gtggtcagct tgtaggtgcc ggggccgaac
     6121 acgtcggcca ccttgccttc gtccacgaac agcgccatct gcgattcgcg caccaccagc
     6181 gtggcgccgt tctggatttc catgcccgcc atcgggtagc gccaggccag cgtgccgtcg
     6241 ctgtcctccg tccattgcag gacgtcgatg aactgcttct tgatgaaatc catcagggcc
     6301 atgggcgctc tccaatcagg gaaaagtgtg tggatgataa gcgccaccca tgacagggct
     6361 atctgggtct ttggcggaat caattggcca tggcaaatga gaatcactat cattaaaacc
     6421 tgttcgcaca ggagttcgtc atggcccgtt tcatccgcaa caaccgccgc atgctgcaaa
     6481 agaccgccac ctattacgtc atgcacatca cggtggcggc cgccgtggcg tatgccgtca
     6541 cgggtgacct gatgatggct tttgcgctca gcctgctgga gccgacggtg caggcggtgg
     6601 cttttttcgt gcacgaaaaa ctctggtcgc gcgccgcgtc cccggccgtg gcggccgcgg
     6661 cctagaatcg tcggcccagc cccaggagac ccgcgcatgc cgcagaacga ccacaaaccc
     6721 accgaagaaa agcgcgccga cctgcgccgc gccgcgctgg actatcacga gtttccggtg
     6781 cccggcaaga tcgccatcgc gccgaccaag cagctggtca accagcacga gctgtcgctg
     6841 gcgtattcgc ccggcgtcgc cgcgccctgc gaggagatcg tcaaggaccc ggcggcggcc
     6901 ttcaagtaca ccgcgcgcgg caacctgatc ggcgtcatca ccaacggcac cgccgtgctg
     6961 ggcctgggcg acatcggcgc gctggccggc aagccggtga tggagggcaa gggcgtgctg
     7021 ttcaagaagt tctcgggcat cgatgtgttc gacatcgaga tcaacgagaa agacccctac
     7081 aagctggtcg agatcatcgc cgcgctggag cccacgtttg gcggcatcaa cctggaagac
     7141 atcaaggcgc ccgagtgctt catcgtcgag cgcgagctgc gcaagcgcat gaagattccg
     7201 gtgttccacg acgaccagca cggcaccgcc atcgtggtcg gtgcggcggt ggtgagcgga
     7261 ctgaaagtgg tgggcaagaa gatcgaggac gtgaagctgg tcacctccgg cgccggcgcc
     7321 gccgcgctgg cctgcgtggg gctgctggtc aagctggggc tgcggcgcga gaacatctgg
     7381 gtgaccgaca tcaagggcct ggtctacgac ggccgcaccg agctgatgga cccggacaag
     7441 gcgatctacg cgcagaagac cgacaagcgc ctgctggccg aagtgatcga cggcgccgac
     7501 gtgtttctgg gcctgtcggc cggcggcgta ttgaagggcg acatggtggc caggatggcg
     7561 ccgcgcccgc tgatcctggc gctggccaac ccctaccccg agatcacgcc cgaggaagtg
     7621 aaggcggtgc gtgatgacgc cgtgatggcc accggccgca ccgactaccc gaaccaggtc
     7681 aacaacgtcc tgtgctttcc gtacatcttt cgcggcgcgc tggattgcgg ggcgaccacc
     7741 atcaccgacg agatggaaat cgccgccgtg cacgccattg ccgacctggc gcaggccgag
     7801 cagagcgagg tggtggcgcg cgcctacgtg ggtgaaaagc tggccttcgg ccccgaatac
     7861 ctgatcccta aaccgttcga cccgcgcctg atgatgaaga tcgcgccggc ggtggccgag
     7921 gccgcggcgg ccagcggcgt ggcccagcgc ccgattgccg acctggacgc ctaccgcgac
     7981 aagctgcaga ccttcgtcta cgcgtcgggc acgacgatga agccgatcat cgacgccgcg
     8041 cgcaacgcca gcaggaagcg cgtggcctat gccgagggcg aggaagagcg cgtgctgcgc
     8101 gcggcgcaga tcgtggtgga cgacggcatt gcgcgcccga cgctgatcgg ccgcccggcc
     8161 atcatcgccc agcgcatcga gaagttcggc ctgcggctga cggaaggcgt ggactacgac
     8221 gtggtgaacg tcgagcacga cgaccgctac aagggcttct ggcagaccta tcacgagctg
     8281 accaagcggc gcggcatcac ggtgccgatc gccaagatcg agatgcgccg gcgcctgtcg
     8341 ctcatcggcg ccatgctgct gcgctcgggc gacgttgatg gcctgatctg cggcacctgg
     8401 ggcaccaacc agatgcacct gacctacatc gacc
//
LOCUS       DAOMVO010000086         8410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2177_length_8410_cov_14.635428, whole genome shotgun sequence.
ACCESSION   DAOMVO010000086 DAOMVO010000000
VERSION     DAOMVO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2177_length_8410_cov_14.635428"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..2163
                     /locus_tag="PKV30_05890"
     CDS             274..2163
                     /locus_tag="PKV30_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL29783.1"
                     /translation="MRDAFRRADKARLASLLPLARGHALEPWAAYWALKARLEEASPL
                     EVQDFLTRYAGTYQQDRLRNDWLLLSGKRRDWASFSAEYPRYRMRDDREVRCYAVAAD
                     IAAGKPASKEAAAQVRRDWFAQRELDDGCLYAAGLLHQAGVLPYQELWKKARLALEAN
                     RASLARAAVALDAPDVAPEVAAIQQSPIRFLTSRAAVGTRKGQELVMLALIKLATQNA
                     DQAAQQLDSKWSVQLGAEERNWAWGVIGKWSALALSDQANGYFAKVTRDADLSDDLLA
                     WKARAALRAGDWRSVQRAVDAMQDPAADDGAWIYWKARAQLALARSDADRAAPRTALE
                     RLAANPGSSGFYEKLAREELGQPLMSPPPPAPLTAEERDAARRHIGLNRALLAIALGL
                     RGDGVREWNYWTNLHVPGGMGDRELLAAADFACARQVWDRCINASERTKSFMDFSQRF
                     PTPHRDAVVRQSQAIGLDPAYVYGLIRQESRFVMDARSGVGASGLMQVMPATARWTAR
                     KIGLDGFVPSQINDRDTNILIGTNYLKLALDDFQGSMPMAAAAYNAGPGRPRNWRNGP
                     VLEGAIWAENIPFNETRDYVKKVLSNTADYAGLFTGQPQSLKRRLGTVGPLAPGEPDM
                     SRDLP"
     gene            2257..3225
                     /locus_tag="PKV30_05895"
     CDS             2257..3225
                     /locus_tag="PKV30_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRL29784.1"
                     /translation="MTQRVLILGGSGFVGRHVVERLQRQGVAMTVPTRRLAAARHLWS
                     QPRVHVTVADVMDPPTLRRLVAEHDAVVNLIAILHGSQRQFERVHVELPRLIADAAAQ
                     AGGRRLVHISALGAAADGPSRYQRSKARGEAVLGQAAQIGTLDLTVLRPSVIFGAEDR
                     FLNLFAGLQKVFPLMPLAAADARFQPVWVRDVAEAVVNGLNDDATIGQTYECCGPEVW
                     TLRELVTAAGQWAGVAGGRGRPVIGLPRALGRLQALALELLPGTPLMSRDNLDSMKLD
                     NVCSGQAPGLQALGIDAAPLSAVGPLYLGQRGRQALLDRYRRGAGR"
     gene            3293..3838
                     /locus_tag="PKV30_05900"
     CDS             3293..3838
                     /locus_tag="PKV30_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRL29785.1"
                     /translation="MSDSLWATVWQTVAAEFSDVPDLSEGLRILLRLSLALLLGLLLG
                     YERESQGKSAGVRTHMLVAMGSALFVLLPSQMGMDIADLSRVVQGVVAGVGFLCAGTI
                     LKHNAAGGDEHVQGLTTAAGLWLTAAIGMACGLGREVTAIMSALLALAVFALIPRLVS
                     PRAAANTDTDAHKAPAADRPG"
     gene            3937..4323
                     /locus_tag="PKV30_05905"
     CDS             3937..4323
                     /locus_tag="PKV30_05905"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL29786.1"
                     /translation="MKLTPLNAPQAPLPAGGYAQAFEVQGAGKLLFISGQIPQTAGGE
                     VPDDFEAQAALVWRNVLAQLHAAGLGVDALVKVTTFLASREHAEANREARQHALGVHT
                     PALTVIITGIFDERWLLEIEAIAAAP"
     gene            4363..5058
                     /locus_tag="PKV30_05910"
     CDS             4363..5058
                     /locus_tag="PKV30_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRL29787.1"
                     /translation="MLKLYIGNKNYSSWSMRPWVLLKQAGIEFDEIMVRFDSFSAGSQ
                     FKNALTGVTPVGKVPVLVDGDLVVWDTLAIAEYVAERFPELHLWPREVPARARARSVC
                     AEMHSGFTALRSACGMNIEARLPDVGALIWRDNAAVRADVARLVQMWQALLAEHGGPL
                     LFGRFTVADAFYAPVVMRLRSFALPVPDDVRTYMDRVSALPGVKAWIDGALAEQDFLD
                     FEEPYRLRATSVR"
     gene            complement(5075..>5475)
                     /locus_tag="PKV30_05915"
     CDS             complement(5075..>5475)
                     /locus_tag="PKV30_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL29788.1"
                     /translation="SGEYAAIRERLGPFDLALLEVGAFHPAWGDMHLGPDHALEALAL
                     LGNPPLLPVHWGTFALAMHDWDQPVERLLELAPGAGAQLVMPRLGEAIEPAHAETVTP
                     WWRGAGSPLPAASRSDADSALPPQVPWPVD"
     assembly_gap    5476..5575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5579..6181)
                     /locus_tag="PKV30_05920"
     CDS             complement(5579..6181)
                     /locus_tag="PKV30_05920"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRL29789.1"
                     /translation="MGKRWQEICIWGALALLPTVSVAETYGAPAPRAEVADRDIRVQR
                     HGNAFSIDAVMHAPVPPATAWEVLTDFEHMPRYQSSLKSSQVLERSPQRVLVQQKGVA
                     RFGPFSNAFESVREMQLTPQRSIRAQQVSGSALKQMASLMTLEPEEGGTRLTYRADME
                     PETALPPLVGPAMVQHETAAQFSAMVREMVRRQGERTAAK"
     gene            6331..7653
                     /locus_tag="PKV30_05925"
     CDS             6331..7653
                     /locus_tag="PKV30_05925"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HRL29790.1"
                     /translation="MQIYMVGGAVRDRLLGLPVQDHDWVVVGATPEQMVAQGYLPVGK
                     DFPVFLHPDSREEYALARTERKSGRGYKGFTVFTGPDVTLEEDLARRDLTINSIAACA
                     DWTSADGQFDSKVDLIDPFGGQRDLQAKVLRHVTDAFREDPVRILRLARFAARFHDFS
                     IAPQTMALLREMVADGEADHLVPERVWQELSRGLMEAHPARMFDVLRECGALAVVLPE
                     LNRLWGVPQRAEYHPEVDTGVHVMMVLQMAARLGASLPVRWACLMHDLGKGTTPADVL
                     PRHIGHEQRSSKLLRTVGQRLRVPTDCTELADVVAREHGNIHRSGDLNAAALVRLLER
                     CDAFRKPARFADALLACECDARGRLGMEEDAYPQRPHLLAALAAAQGVDTASVAQAAV
                     DRGARGDAIGKAVATARAQAVQAWLNQAASETPSGDGCTAAPKEPPTR"
     gene            7869..>8410
                     /locus_tag="PKV30_05930"
     CDS             7869..>8410
                     /locus_tag="PKV30_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRL29791.1"
                     /translation="MARPISASHDLRRDEILNVAAACFAGQSYPATSMNHIAAACGTS
                     KARLYHYYESKEAILFDLLDRYTQKLLLIIGQVEATAQRRALDERASLHELIRAFLRE
                     YETSATRHAALLGSTQFLSEQQREVILDRQRDVVAAVTRFLRRAYPDRLNEHNQTAIA
                     MTLFGMINWTFTWLRPGGPL"
BASE COUNT         1253 a         2899 c         2883 g         1275 t
ORIGIN      
        1 gtcgaacttg ctcataatgg gtcgatgcgt ttttcaaaga ttctgacacc gttgcaactg
       61 ggcggcgtcg ccttgttgtg cggcgcggcg gcgctggcgc agcccggcag cccgccgcag
      121 acctatgcgc cgccggccgc gttgcccggt gcacccgcgc ccgcgccgct gccggtggct
      181 cctgtgctgc ccctgctgcc cgctgaaccc gtcgccacgc ccgttgcgcc gccgctggca
      241 accgccgcac ccagcgacag cgtgatcctg gacatgcgcg acgcctttcg ccgtgccgac
      301 aaggcgcgcc tggccagcct gctgccgctg gcacgcggcc atgcgctgga accctgggcc
      361 gcctactggg cgctgaaagc gcggctggaa gaagccagcc cgctggaagt gcaggacttc
      421 ctcacccgct acgccggcac gtatcagcaa gaccgcctgc gcaacgactg gctgctgctg
      481 tcgggcaagc ggcgcgactg ggcctctttc agcgccgaat acccgcgtta ccgcatgcgc
      541 gacgaccgcg aggtgcgctg ctacgccgtg gcggccgaca tcgccgccgg caagcccgcc
      601 agcaaggaag cggccgcgca ggtgcggcgc gactggttcg cccagcgcga actggacgac
      661 ggctgccttt acgccgccgg cctgctgcac caggccggcg tgctaccgta tcaggagctg
      721 tggaagaagg cccgacttgc gctggaggcc aatcgggcca gcctggcgcg cgccgccgtg
      781 gcgctggacg cgcccgacgt ggcgcctgaa gtcgccgcca tccagcaaag cccgatccgc
      841 tttctgacat cgcgggcggc ggtgggcacg cgcaaggggc aagagctggt gatgctcgcg
      901 ctcatcaagc tggcgacgca gaacgccgac caggcggcgc agcagctcga cagcaaatgg
      961 agcgtgcagc tcggcgccga agagcgcaac tgggcctggg gcgtgatcgg caagtggtcg
     1021 gcgctggccc tgtccgacca ggccaacggc tatttcgcca aggtcacccg cgacgccgac
     1081 ctgagcgacg acctgctggc ctggaaagcg cgcgccgccc tgcgcgccgg cgactggcgc
     1141 agcgtgcagc gcgccgtcga cgccatgcag gatccggccg ccgacgatgg cgcctggatc
     1201 tactggaagg cccgtgcgca gctggcgctg gcccgcagcg atgccgaccg cgccgcgccg
     1261 cgcaccgcgc tggagcgcct ggccgccaac cccggcagct cgggctttta cgaaaaactg
     1321 gcgcgcgaag aactcggcca gcccctgatg tcgcccccgc cacccgcgcc gctcaccgcc
     1381 gaagagcgcg acgccgcgcg ccgccacatc ggcctgaacc gcgcgctgct ggccatcgcg
     1441 cttggcctgc gcggcgacgg cgtgcgcgaa tggaactact ggaccaacct gcacgtgccc
     1501 ggcggcatgg gcgaccgcga gctgctggcc gccgccgact ttgcctgcgc gcgccaggtg
     1561 tgggaccgct gcatcaacgc cagcgagcgc accaagtcct tcatggactt cagccagcgc
     1621 tttccgaccc cgcaccgcga cgcggtggtg cgccagtcgc aggccatcgg gctcgacccg
     1681 gcttacgtct acggtctgat ccggcaggaa agccgcttcg tgatggacgc gcgctcgggc
     1741 gtcggcgcct cgggcctgat gcaggtcatg cccgccaccg cgcgctggac ggcgcgcaag
     1801 atcgggctgg acggctttgt gccgagtcag atcaacgacc gcgacaccaa catcctgatc
     1861 ggcaccaact acctcaagct ggcgctggac gacttccagg gctcgatgcc gatggccgcc
     1921 gccgcctaca acgccggccc gggccggccg cgcaactggc gcaacgggcc ggtgctggaa
     1981 ggcgccatct gggccgagaa catcccgttc aacgagacgc gcgactacgt gaagaaggtg
     2041 ctgtcgaaca ccgccgacta cgccggcctg ttcaccggcc agccgcagtc gctcaagcgg
     2101 cggctgggca ccgttggccc gctggcgccc ggcgagccag acatgagccg cgatttgcct
     2161 tgaccgccgc cttggcgccg gcacttaatt tattggtcaa atcggccgct ggcgcttgtt
     2221 ggataagcgc aagcagctat tgattcagga gtatccatga cccagcgtgt gctcatcctc
     2281 ggcggttccg gcttcgtcgg ccgccacgtt gtcgaacggc tgcagcgcca gggcgtggcc
     2341 atgacggtgc ccacacgccg cctggccgcc gcgcgccacc tgtggagcca gccgcgcgtg
     2401 cacgtcacgg tggccgatgt gatggacccg ccgacgctcc ggcgcctggt ggcggaacac
     2461 gacgcggtgg tcaacctgat cgccatcctg cacggcagcc agcggcagtt cgagcgcgtg
     2521 cacgtcgagc tgccgcgcct gatcgccgac gccgcggcgc aggcgggtgg ccggcggctg
     2581 gtgcacatca gcgcgctcgg tgcggcggcc gatggcccgt cgcgctacca gcgcagcaag
     2641 gcgcgcggcg aggccgtgct gggccaggcc gcgcagatcg gcacgctgga tttgacggtg
     2701 ctgcgcccga gcgtgatctt tggcgccgaa gaccgttttc tgaacctgtt tgccggcctg
     2761 caaaaggtgt ttccgctgat gccgctggcg gcggccgacg cgcgctttca gccggtgtgg
     2821 gtgcgcgacg tggccgaagc cgtggtcaac ggcctgaacg acgacgccac cataggccag
     2881 acctatgaat gctgcggccc cgaggtgtgg acgctgcgcg aactcgtcac cgccgccggt
     2941 cagtgggccg gcgtggcggg cggccgcggg cgccccgtca tcggcctgcc gcgcgcgctg
     3001 ggtcgtctgc aggcgctggc gctggaactg ctgcccggca cgccgctgat gagccgcgac
     3061 aacctggatt cgatgaagct cgacaacgtg tgcagcggcc aggcgccggg gctgcaggcg
     3121 ctgggcatcg acgccgcgcc gctgtcggcc gtcggcccgc tgtacctggg ccagcgcggc
     3181 cggcaggcgc tgctcgaccg ctaccggcgc ggcgccgggc gctgaagcgg cgcggcgcgg
     3241 acgcgcgctc ggccgacaga cgcgccccag ccgccatggc acgctggcgc gcatgagcga
     3301 ttcactctgg gccacggtgt ggcagacggt ggcggccgaa ttctccgacg tgcccgatct
     3361 gagcgagggc ctgcgcatcc tgctgcgcct gtcgctggcc ttgctgctgg gcctgctgct
     3421 gggttatgag cgcgaaagcc agggcaaatc ggccggcgtg cgcacccaca tgctggtggc
     3481 catgggctcg gccctgttcg tgctgctgcc gagccagatg ggcatggaca tcgccgacct
     3541 gagccgcgtg gtgcagggcg tggtggcggg tgtgggcttc ctgtgcgccg gcaccatcct
     3601 caagcacaac gccgccggcg gcgacgagca tgtgcaaggc ctgaccaccg ccgccggcct
     3661 gtggctgacg gccgccatcg gcatggcctg cggcctgggc cgcgaggtga cggccatcat
     3721 gagcgcgctg ctggcgctgg ccgtgtttgc cttgattccg cgcttggtga gcccacgtgc
     3781 tgcggccaac accgacacgg acgcccacaa agcgccagcc gccgaccgcc cgggctgagc
     3841 ggcgccgcgc ggccctcgca tcaatggccg cgccaacttg catcaatcgg ctggggttgg
     3901 cgcgctagca tgtgcccttc gccattggag agccgcatga aattgacacc gttgaacgcg
     3961 ccgcaggccc cgttgccggc gggtggctat gcgcaggcct tcgaagtcca gggcgccggc
     4021 aagctgctgt tcatcagtgg ccagattcca cagaccgccg gtggcgaggt gcccgatgat
     4081 ttcgaggcgc aagccgcgct ggtgtggcgc aacgtgctgg cgcaactgca cgccgccggg
     4141 ctgggcgtgg acgcgctcgt gaaagtcacc acctttctgg cgtcgcgtga gcacgccgag
     4201 gccaaccgtg aagcccgtca gcacgcgctc ggcgtccaca cgccggcgct gacggtgatc
     4261 atcaccggca tcttcgacga acgctggctg cttgaaatcg aagccatcgc cgccgcgccc
     4321 tgatctgaca atcgctcgtt cgcccacccc gaaagtctga ccatgctcaa gctctacatc
     4381 ggcaacaaga attactcgtc ctggtccatg cggccatggg tgctcctcaa gcaggccggc
     4441 atcgagttcg acgaaatcat ggtgcgcttc gactcgttca gcgccggttc gcagttcaag
     4501 aacgcgctga cgggcgtcac cccggtgggc aaggtgccgg tgctggtcga cggcgatctg
     4561 gtggtgtggg acacgctggc gatcgccgaa tatgtggctg agcgctttcc cgagctgcac
     4621 ctgtggccgc gcgaggtgcc ggcacgtgcc cgcgcgcgca gcgtgtgcgc cgaaatgcac
     4681 agcggcttca ccgccttgcg cagcgcctgc ggcatgaaca tcgaggcgcg cctgcccgat
     4741 gtgggcgcgc tgatctggcg cgacaacgcc gccgtgcgcg cggacgtggc gcgcctggtg
     4801 cagatgtggc aggcgctgct ggccgaacac ggcggcccgc tgctgttcgg ccgcttcacg
     4861 gtggccgacg cgttctacgc gcccgtcgtc atgcgcctgc gcagctttgc gctgccggtg
     4921 ccggacgacg tgcgcaccta catggaccgc gtgtctgcgc tgcccggcgt caaggcgtgg
     4981 atcgacggcg cgctggccga gcaggatttt ctggatttcg aggagccgta ccggctcagg
     5041 gcgacgtcgg tccgctgacg cgccggcgcg cgtgtcagtc cacgggccac ggcacctgtg
     5101 gcggcagcgc gctgtcggcg tcgctgcgcg acgcggcggg cagcggcgat ccggcgccgc
     5161 gccaccacgg cgtgacggtt tcagcgtgcg cgggctcgat ggcctccccc agccgtggca
     5221 tcaccaactg cgcgcctgcg ccgggcgcca gttcgagcaa gcgttcgacc ggctgatccc
     5281 agtcgtgcat cgccagcgca aaggtgcccc agtgcactgg cagcagcggc gggttgccca
     5341 gcagcgccag cgcttccagc gcgtgatccg gccccaggtg catgtcgccc caggcggggt
     5401 gaaaggcgcc gacttcgagc agcgccagat cgaacgggcc cagccgctcg cggatggcgg
     5461 cgtattcgcc cgacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggct
     5581 acttggcggc cgtgcgttcg ccttgccggc gcaccatttc gcgcaccatg gcggagaact
     5641 gcgccgccgt ctcgtgctgc accatggcgg ggccgaccag cggcggcagg gcggtttcgg
     5701 gctccatgtc ggcgcggtaa gtcaggcgcg tgccgccttc ctcgggctcc agcgtcatca
     5761 ggctggccat ttgcttgagc gcggaaccgc tcacctgctg ggcacggatg ctgcgctgcg
     5821 gcgtcagctg catctcgcgc accgactcga aggcgttgct gaacgggccg aagcgcgcca
     5881 cgcccttttg ctgcaccagc acgcgctgcg ggctgcgctc cagcacctgg ctgcttttca
     5941 ggctgctttg gtagcgcggc atgtgttcga aatcggtcag cacctcccac gcggttgccg
     6001 gcggcacggg tgcgtgcatc acggcgtcga tgctgaaggc gttgccgtgg cgctgcacgc
     6061 ggatgtcgcg gtcggccacc tcggcgcgcg gcgcgggcgc gccgtaggtt tctgccacag
     6121 acacggtggg cagcaaggcc aatgcccccc agatgcagat ctcttgccaa cgcttgccca
     6181 tgtttgcctt cggtgtcgcg accacaaggc gcccaatatg ccgcgccggc ggcgcgcgcc
     6241 gcatcagcca atcggcgtag ggcgtgtcgg ccaaaagctc aggcgccagg cagggtggct
     6301 ttcaggtggg caagccctaa actgcgccgc atgcaaatct acatggtcgg cggggcggtg
     6361 cgtgaccgct tgctcgggct gccggtgcag gaccacgact gggtggtggt gggcgccacg
     6421 cccgagcaga tggtggcgca gggttatctg ccggtgggca aggatttccc ggtctttttg
     6481 cacccggact cgcgtgagga atacgcactg gcgcgcaccg agcgcaagag cgggcgcggg
     6541 tacaagggct tcacggtctt caccggcccc gacgtgacgc tggaagaaga cctggcgcgg
     6601 cgcgacttaa ctatcaattc aatagctgct tgcgctgatt ggacaagcgc tgacggccaa
     6661 tttgattcaa aagtcgatct gatcgacccg ttcggcggcc agcgcgatct gcaggccaag
     6721 gtgctgcgcc acgtgaccga cgccttccgc gaagacccgg tgcgcatcct gcggctggcg
     6781 cgctttgcgg cgcgttttca cgacttttcc atcgcgcccc aaaccatggc actgctgcgc
     6841 gaaatggtgg ccgacggcga ggccgaccac ctggtgcccg agcgcgtgtg gcaagagctg
     6901 tcgcgcggcc tgatggaagc gcacccggcg cgcatgttcg acgtgctgcg cgagtgcggt
     6961 gcgctcgccg tggtgctgcc cgagctgaac cgcctgtggg gcgtgccgca gcgcgccgaa
     7021 taccaccctg aagtcgacac cggcgtacac gtgatgatgg tgctgcaaat ggccgcgcgc
     7081 ctgggcgcca gcctgccggt gcgctgggcc tgcctgatgc acgacctggg caagggcacc
     7141 acgccggccg acgtgctgcc gcgccacatc ggccacgagc agcgcagcag caagctgttg
     7201 cgcaccgtgg gccagcgcct gcgcgtgccg accgactgta ccgaactggc cgacgtggtg
     7261 gcgcgcgagc acggcaacat ccaccgcagc ggcgacttga acgccgccgc gctggtgcgc
     7321 ctgctggagc gctgcgacgc cttccgcaag ccggcgcgct ttgccgacgc gctgctggcc
     7381 tgcgaatgcg acgcgcgcgg gcgcttgggc atggaagaag acgcctaccc gcagcgcccc
     7441 catctgctgg ccgcgctggc ggcggcgcag ggcgtggaca cggccagcgt ggcgcaagcg
     7501 gcggtggatc gcggcgcgcg tggcgatgcc attggcaagg cggtggccac ggcgcgcgcg
     7561 caggcggtgc aggcgtggct caatcaggcg gcgtctgaaa cgccttcagg tgatgggtgc
     7621 acagccgcgc caaaagaacc tcctacccgt tagtcctgag cttgtcgaag gacttgggtt
     7681 gcacaaaaaa gggcttcgac aggcctgtca tgagccaagt cgaatggctc agcccgatcg
     7741 gatgatgggc tgccgaaccg ttcgtgagtc agctttcaaa aacatagctg ctcgcgctgg
     7801 tccctcaagc gctggcggcc gatttggctc cgaaaccggc atgcgctgcc gcagccgata
     7861 atccgcccat ggcccgcccc atctccgcct ctcacgacct gcggcgcgac gaaatcctga
     7921 acgtcgcggc ggcttgcttt gccggtcaaa gctacccggc caccagcatg aaccacattg
     7981 ccgccgcctg cggcacgtcg aaggcgcggc tgtatcacta ttacgaaagc aaggaagcga
     8041 tcttgttcga cctgctggac cgctacacgc aaaagctgct gctcatcatc ggccaggtcg
     8101 aggcgacggc gcagcgccgc gcactggacg agcgcgccag tctgcacgag ttgatccgcg
     8161 cctttttgcg cgagtacgaa acctcggcca cgcgccacgc cgcgctgctg ggcagcacgc
     8221 aattcttgag cgagcagcag cgcgaggtga tcctcgaccg ccagcgcgac gtggtggccg
     8281 ccgtcacgcg ctttctgcgc cgcgcctacc cggatcggct gaacgagcac aaccagaccg
     8341 ccatcgccat gacgctgttt ggcatgatca actggacctt cacctggctg cgcccgggcg
     8401 gcccgctcag
//
LOCUS       DAOMVO010000087         8203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2261_length_8203_cov_14.790501, whole genome shotgun sequence.
ACCESSION   DAOMVO010000087 DAOMVO010000000
VERSION     DAOMVO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_8203_cov_14.790501"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKV30_05935"
     CDS             complement(<1..294)
                     /locus_tag="PKV30_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29792.1"
                     /translation="MQTTMMNAPLSLNHLLERAGQIFHGNDIVSRLPDKSLKRHSYAQ
                     YYRRTRALSAALLELGLQKGECVATLCWNHHAHLEAYFGIPAAGGVMHTLNLRL"
     gene            367..963
                     /locus_tag="PKV30_05940"
     CDS             367..963
                     /locus_tag="PKV30_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29793.1"
                     /translation="METLTHEIAAHAARLVVEEGLDYGAAKRRAQKTLGLPPRTALPD
                     NDLLEAEVRAYIALFYADTQPAELAALRRLALLWMDRLAEFRPHVAGAVWNGTATRLS
                     DIHLQLFCDDPKSAEIALIDHRVAYQPRSVTGFNGAQVDALSFSSPCPALGEPIGVHL
                     MIHDHDDLRGALKPGPRGQPRGDRRALAARLQESEQTT"
     assembly_gap    1136..1235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1478..1963
                     /gene="accB"
                     /locus_tag="PKV30_05945"
     CDS             1478..1963
                     /gene="accB"
                     /locus_tag="PKV30_05945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRL29794.1"
                     /translation="MDLRKLKTLIDLVSESNVAELEITEAEGKVRIVKSNPAAQPVVY
                     GAALPAPVAAPVTATTAAPVAAAPVAAGSTPAPAAAEEPTGFVVKSPMVGTFYRAASP
                     GAKAFVEVGQQVKEGDTLCIVEAMKILNEIDAEKSGTITKVLGENGQAVEYGQPLFVI
                     E"
     gene            2141..3490
                     /gene="accC"
                     /locus_tag="PKV30_05950"
     CDS             2141..3490
                     /gene="accC"
                     /locus_tag="PKV30_05950"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRL29795.1"
                     /translation="MFKKILVANRGEIALRVQRACRELGVKAVMVYSEADKDAKYVRL
                     ADEAVCIGPAPSGQSYLSMPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFQF
                     IGPTPESIRIMGDKVSAKQAMIKAGVPCVPGSEGELPDDPTAIKRIAKSIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLHAVQTTKAEAAAAFNNPAVYMEKFLQNPRHIEIQILADK
                     HRNAVYLGERDCSMQRRHQKVIEEGPAPGIPRRLIERIGERCVAACKKIGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTEWITGVDIVRTQIMVAAGEKLPFTQRQISIKGHA
                     IECRVNAEDPFKFTPSPGRVTAWHMPGGPGVRVDSHVYNNYFVPPNYDSMIGKIIVYG
                     DTREQAIARMQTALAETVVEGIQTNIPLHRELMVDAKFMEGGTNIHYLEKWLGDRQR"
     gene            3561..4445
                     /gene="prmA"
                     /locus_tag="PKV30_05955"
     CDS             3561..4445
                     /gene="prmA"
                     /locus_tag="PKV30_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRL29796.1"
                     /translation="MYELRLICPEERVEQISDALEALEALSVSVEDADADTDAERALF
                     GEPGLPPPAQSWSRSRVVALFGSEADAHQAARLLTLQDFFDGCVVLQIEPVAEHDWVR
                     LTQSQFEPVPITERFWIVPTWHDVPPTATEVIRLDPGLAFGTGTHPTTRMCLRWIAAH
                     PPRGQRVLDYGCGSGILAIGAALHGAASVDAVDIDEAAVQSTRDNAAANGVSLVAGLP
                     DQAQGSYDTVLANILATPLKVLAPLLCGHVAAGGALVLAGVLERQTAELQAAYQPWLA
                     LEVADAEDGWVLLVGRRT"
     gene            4482..>5200
                     /locus_tag="PKV30_05960"
     CDS             4482..>5200
                     /locus_tag="PKV30_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRL29797.1"
                     /translation="MSLITRCPACATMFKVVRDQLRISGGWVRCGQCAEVFDASAHMV
                     VEGALVAAAPPPPQPAPMPRSESTVVQRESVPEAPASRAAGAPKAGVASAPVATSVEP
                     AAPVAAPPRQPSWWTPSAAWSKPSAAPSPTDDGAPEKVPQREVDQRLDQPLPDAASAP
                     SDVPAAGVPWTASELMTWRELEEAAEAERASAPPATPATLDDAPAPAQQEVSLVVEPP
                     LAQSVDPVEEVVVAEVLSPEPA"
     assembly_gap    5201..5300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5301..6025
                     /locus_tag="PKV30_05965"
     CDS             <5301..6025
                     /locus_tag="PKV30_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRL29798.1"
                     /translation="VDPVEEVVVAEVLSPEPASESEPESEPETAASPSAMEVDFVLSD
                     VGRLDDDAPPASAPAVQQEHDPLVAPAFVAQARRRAFWSSRPVRAVLWLIALLLALGL
                     ALQWVVSQRDWLAAREPRLVPLLQAVCEPLGCRLAPYRMPDAVVIDSSAFNRVNTNTF
                     RFGVTLRNMADIDVATPALELTLTDAQDQPLLRRVVSAAELGAPATLGAHGEFSGTNE
                     LKVNDIPNPGAIVGYRLMAFYP"
     gene            6083..6988
                     /locus_tag="PKV30_05970"
     CDS             6083..6988
                     /locus_tag="PKV30_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRL29799.1"
                     /translation="MATLICGSLAFDTIMSFEGRFAEQILPDQLHILNVSFLVPDLRR
                     DFGGCAGNIAYAMQQMGGAPLPMATLGNDGADYLERLRTLGIDTGFVRQIDDTYTAQA
                     MIMTDRDNNQITAFHPGAMMQAHRTRIEARADLRLGIVSPDGRDAMLEHAAQFHAAGI
                     PFVFDPGQGLPMFGGAELARFVELATWVTVNDYEGRMLCERMDVDLAELSRRVQGLVV
                     TLGGEGCDLWQDGQRTHVPAVQPEAVVDPTGCGDAWRGALLYGLEQGWPLARCAALGN
                     RLGALKIASRGPQNYRVAPSILTDL"
     gene            complement(7144..7656)
                     /locus_tag="PKV30_05975"
     CDS             complement(7144..7656)
                     /locus_tag="PKV30_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like DNA-binding protein"
                     /protein_id="HRL29800.1"
                     /translation="MATAKKAPAKKAAAKKAPAKKAPANKVAAKKAAPAKKVVAKKAP
                     AKKVLAKKVVAKKAPAKKAAPAKKAAAKKAPAKKVMAKKAPAKKAAPAKKAVAKKAPA
                     KKVAAKKAAPAKKAVAKKAPAKKAAPAKKAAPAKKAAAKKAPARKPAPPAQTTLNPQA
                     AWPFPTAAKP"
     gene            complement(7830..>8203)
                     /locus_tag="PKV30_05980"
     CDS             complement(7830..>8203)
                     /locus_tag="PKV30_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRL29801.1"
                     /translation="DESMHCNFGIDLINQIKLENPHLWTPAFKAELKGLFERAVELEY
                     RYAEDTMPRGVLGMNASMFKGYLRYIANRRATQIGLEALFPNEENPFPWMSEMIDLKK
                     ERNFFETRVIEYQSGGALSWD"
BASE COUNT         1302 a         2631 c         2658 g         1412 t
ORIGIN      
        1 caggcgcagg ttgagcgtgt gcatcacgcc gccggcggcg ggaatgccga agtaggcttc
       61 caggtgggcg tggtggttcc agcacagcgt ggccacgcac tcgccttttt gcagccccag
      121 ctcaagcaac gccgccgaca gggcgcgggt tcggcggtag tactgggcgt agctgtggcg
      181 cttgaggctc ttgtcgggca ggcgcgagac gatgtcgttg ccgtgaaaga tctgccccgc
      241 gcgctccagc agatggttga gcgacagcgg cgcgttcatc atcgtggtct gcatggcttt
      301 gtctccgatt ggggccatcg gcaactctac ggcaacgcgg cgccggcgca aggcaaaatc
      361 cggcgcatgg aaaccctgac gcacgagatc gctgcgcacg ccgcgcggct ggtggtggaa
      421 gaagggctgg actacggcgc cgccaagcgc cgcgcgcaaa agacgctggg cctgccgccg
      481 cgcaccgcgt tgcccgacaa cgatttgctc gaagccgagg tgcgggccta catcgcgctg
      541 ttttacgccg acacccagcc cgctgaactg gccgccctgc gccgcttggc cctgttgtgg
      601 atggaccggc tggcggagtt caggccgcat gtggccggcg cggtgtggaa cggcaccgcc
      661 acccgcctgt ccgacattca cctgcagctg ttttgcgacg acccgaagtc ggccgaaatc
      721 gcgctgatcg accaccgcgt ggcctaccag ccgcgcagcg tgaccggatt caatggcgcg
      781 caggtcgacg cgctgagctt ctccagccca tgccccgcac tgggcgaacc gataggcgtg
      841 cacctgatga ttcacgacca cgacgacttg cgcggcgccc tgaagcccgg gccgcgcggc
      901 cagccccgcg gcgaccgccg tgcgctggcc gcgcgcctgc aggaaagtga gcagacgaca
      961 tgagcgtgca acctgatgcc gccgatggcc gcgccgcgcc tgaatcaccc gccgcaccgc
     1021 gcaagacgcg ccgtgcggcg ctggccgcag tcgcccttgc ggcggtggcc gccggcgctg
     1081 tggtgggctg gcgccgattc gagttgagcg aagccgacat cggcagtttc tgggannnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcga gttgggaaat gcggccggcg
     1261 gtttgccgtt cagcgtgctt tttgacgccg gtgggcgcct gcgcgagcgc aagctcggtc
     1321 agttgcacga ggctgacctg aaggcgtggc gggcgggcct gcaagcctga cagggcatgt
     1381 gtgccgaccg aaaattcggc gaaattgaat caagatcacc gcttttgtgt ctaaattgat
     1441 gtaaattcgc ccgcattctc ccaatctgga agtttcaatg gatttgcgca aactgaagac
     1501 gctgatcgac ctggtgtcag agtcgaacgt ggcggagctg gaaatcaccg aggccgaagg
     1561 caaggtgcgc atcgtcaaga gcaacccggc ggcacagccg gttgtgtacg gtgcggcgct
     1621 gccggcaccc gtggcagcgc ccgtcacggc cacaaccgcc gcaccggttg cggctgcgcc
     1681 agtggcggcc ggcagcacgc ccgctcccgc ggcggccgag gaacccaccg gcttcgtcgt
     1741 caagtcgccg atggtcggca ccttctaccg cgcggccagc ccgggcgcca aggccttcgt
     1801 cgaagtcggc cagcaggtca aggagggcga cacgctgtgc atcgtcgagg cgatgaagat
     1861 ccttaacgag atcgatgccg aaaagagcgg caccatcacc aaggtgctgg gcgagaacgg
     1921 ccaggcggtc gagtatggcc agccgctgtt tgtaattgaa tgagcatccc cctgagcggc
     1981 ttacgccgct tcccccttct ctcgctgcgc gggaaggggg acgccgctgg cggtcgggga
     2041 aaccccgacc acggcggcct cgcttggcct gctccgcggc cttttgaagg gtgcgtggct
     2101 cgcgcgatgt ttgcgtctcg ctacgcaatg gataactgac atgttcaaaa aaatactggt
     2161 ggccaaccgc ggcgaaatcg cgctgcgcgt gcagcgcgcc tgccgcgaac tgggcgtgaa
     2221 ggccgtgatg gtttattccg aggccgacaa ggacgccaag tacgtgcgcc tggccgacga
     2281 ggcggtgtgc atcggaccgg cgccttcggg gcaaagctac ctcagcatgc cagccatcat
     2341 ttcggcggcc gaagtgaccg atgccgaggc gattcacccc ggctacggtt tcttgagcga
     2401 aaacgccgac tttgccgagc gggtggagaa aagcgggttt cagttcatcg gcccgacgcc
     2461 ggagtcgatc cgcatcatgg gcgacaaggt gtcggccaag caggccatga tcaaggccgg
     2521 cgtgccctgc gtgccggggt ctgaaggcga actgcccgac gacccgacgg cgatcaagcg
     2581 catcgccaag tcgattggct acccggtcat catcaaggcg gcgggcggcg gtggcgggcg
     2641 cggcatgcgc gtggtgcaca ccgaggcggc gctgctgcat gcggtgcaaa ccaccaaggc
     2701 cgaggcggcg gcggccttca acaacccggc ggtgtacatg gagaagtttc tccagaaccc
     2761 gcgccacatc gagatccaga tccttgccga caagcaccgc aacgcggtct acctgggcga
     2821 gcgcgattgc tccatgcagc ggcgccacca gaaggtgatc gaagaggggc cggcaccggg
     2881 cattccgcgc cgcctgatcg agcgcattgg cgagcgctgc gtggccgcct gcaagaaaat
     2941 cggctaccgc ggcgccggca ccttcgagtt tttgtacgag aacggcgagt tctatttcat
     3001 cgagatgaac acgcgtgtgc aggtcgagca cccggtgacc gaatggatca ccggcgtcga
     3061 catcgtgcgt acgcagatca tggtggcggc gggcgagaag ctgcccttca cgcagcggca
     3121 gatttcgatc aagggccacg ccatcgagtg ccgcgtgaat gccgaagacc cgttcaagtt
     3181 cacgccctcg cccggccgcg tcaccgcgtg gcacatgccg ggcgggccgg gcgtgcgggt
     3241 cgattcgcac gtctacaaca actacttcgt gccgcccaac tacgactcga tgatcggcaa
     3301 gatcatcgtc tatggcgaca cgcgcgagca ggccatcgcc cgcatgcaga ccgcgctggc
     3361 cgagaccgtg gtcgagggca tccagaccaa catcccgctg caccgcgaac tgatggtgga
     3421 cgccaagttc atggaaggcg gcaccaacat ccactacctt gaaaagtggc tgggtgatcg
     3481 gcagcgttga ttcgctgttt ttgatgaaaa tcggctctgg cgcttgtctg acaagcgcga
     3541 gcagctagca aaataatagt atgtacgagt tgcgcctgat ctgcccggaa gagcgcgtcg
     3601 agcagatctc cgacgcgctg gaggcgcttg aagccttgag cgtctcggtc gaagacgccg
     3661 atgccgacac cgatgccgag cgcgccctgt ttggcgaacc cggcctgccg ccgccagcgc
     3721 agagctggtc gcgctcgcgc gtggtggcgc tgtttggcag cgaggccgat gcgcaccaag
     3781 ccgcgcgttt gctgaccttg caggacttct tcgacggctg cgtggtactg cagatcgaac
     3841 ccgtggccga gcacgactgg gtgcgcctga cgcaatcgca gttcgagccg gtgccgatca
     3901 ccgagcgttt ctggatcgtg cccacctggc acgacgtgcc gccaactgcc accgaagtga
     3961 tccggcttga cccgggcctc gcctttggca ccggcacgca ccccaccacg cgcatgtgcc
     4021 tgcgctggat cgccgcacac ccgccgcgcg gccagcgcgt gctcgactac ggttgtggct
     4081 ccggcatcct ggccattggc gccgcgctgc acggcgccgc cagcgtcgac gcggtcgaca
     4141 tcgacgaagc ggccgtgcaa tccacgcgcg acaacgccgc cgccaacggc gtgtcactgg
     4201 tggccggctt gcccgaccag gcgcagggca gttacgacac cgtgctggcc aacatcctcg
     4261 ccacgccgct caaggtgctg gcgccgctgc tgtgcggcca cgtcgcagcc ggcggcgcgc
     4321 ttgtgctggc cggcgtgctg gagcgccaga cggccgaact gcaggcggcg tatcagccct
     4381 ggctggcgct cgaagtggcc gatgccgagg acggctgggt gctgctggtc ggccgccgca
     4441 cctgagccgg cagcgccaac gcgctgccta caatcgccgc catgagtctg atcacgcgct
     4501 gccccgcctg cgccaccatg ttcaaggtgg tgcgcgatca gctgcgcatc tcgggtgggt
     4561 gggtgcgctg cggccagtgc gccgaggtgt tcgacgcgtc ggcacacatg gtggtcgagg
     4621 gcgcgcttgt ggcggcggcg ccgcctccac cacagcccgc cccgatgccg cgcagcgaat
     4681 cgacggtggt gcagcgcgag agtgtcccgg aggcgccggc ctcgcgcgct gccggtgcgc
     4741 cgaaggctgg cgtggcctct gcgccggtcg caacttctgt cgagcctgct gcgccggtcg
     4801 ccgcgccgcc gcgccagcca tcgtggtgga cgccctccgc ggcctggtcg aagccgagcg
     4861 cagcaccttc gcccacagac gatggcgcgc cggaaaaagt cccgcagcgc gaggtcgacc
     4921 agcgccttga tcaaccgctg ccggacgccg cgtctgcgcc ctctgacgtg cccgccgccg
     4981 gtgtgccatg gacggcgtcc gagctgatga cctggcgcga gctggaagaa gccgccgaag
     5041 cggagcgcgc atcagcgcct ccagcgacgc cagcaacgct ggacgacgcg ccggcgccag
     5101 cacagcagga agtttcgttg gtagtcgagc ctcccctcgc gcagtccgta gacccggtgg
     5161 aagaggtcgt cgttgcggaa gtcttgtcac cagaacccgc nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn ccgtagaccc ggtggaagag gtcgtcgttg cggaagtctt
     5341 gtcaccagaa cccgcatccg aatccgagcc ggagtctgag cccgaaaccg ccgcctcgcc
     5401 atcggcgatg gaggtcgatt tcgtgctgag cgacgtgggc agactggatg acgacgcccc
     5461 tccagcatcg gcacccgctg tccagcaaga gcacgatccg ctggtcgcgc ccgcgttcgt
     5521 cgcccaggcg cgccgccgcg cgttctggtc gtcgcgcccc gtgcgtgcgg tgctttggct
     5581 gattgcgctg ctgcttgcgc tggggctggc cttgcagtgg gtggtcagcc agcgcgactg
     5641 gctggcggcg cgcgagccgc ggctggtgcc actgctgcaa gccgtttgcg agccgctggg
     5701 ttgtcgactg gcgccttacc ggatgccgga tgcggtggtg attgacagct ctgccttcaa
     5761 ccgcgtcaac accaacacct tccgcttcgg cgtcacgctg cgcaacatgg ccgacatcga
     5821 cgtggccacg cccgcgctgg agttgacctt gacggatgcg caagaccagc cgctactgcg
     5881 ccgcgtggtg agcgcggccg aactgggcgc gccggcgacg ctgggtgcgc acggcgagtt
     5941 cagcggcacc aacgagttga aggtcaacga catccccaat cccggcgcca tcgtcggcta
     6001 tcgcctgatg gccttctatc cctgacgcgg tcgcggccgt gtcgccgctg cacctttttc
     6061 attcttgtct gactgattca ccatggccac cctgatctgc ggctcgctcg cgttcgacac
     6121 catcatgagc ttcgagggcc gctttgccga gcaaatcctg cccgatcagc tgcacatcct
     6181 caacgtgtct tttctggtgc ccgacttgcg gcgcgacttt ggcggttgcg ccggcaacat
     6241 cgcctacgcc atgcagcaaa tgggcggcgc gcctttgcca atggccactc tgggcaatga
     6301 cggtgccgac tatcttgagc gcctgcgcac gctgggcatc gacaccgggt tcgtgcgcca
     6361 gatcgacgac acctacaccg cgcaggccat gatcatgacc gaccgtgaca acaaccagat
     6421 caccgccttt caccccggcg ccatgatgca ggcgcaccgc acccgcatcg aggcgcgcgc
     6481 ggatctgcgt ctgggcatcg tctcgcccga cgggcgcgat gccatgctgg aacatgcggc
     6541 gcagtttcac gccgccggca tccccttcgt gtttgacccc ggccaagggc tgccgatgtt
     6601 tggcggcgcc gaactggcgc gctttgtcga actcgccacc tgggtcacgg tgaacgatta
     6661 cgaaggccgc atgttgtgcg agcgcatgga cgtcgatctg gccgagttgt cgcgccgggt
     6721 gcagggcctg gtcgtgacgt tgggcggcga gggttgcgac ctctggcaag acggccagcg
     6781 cacccacgtg cccgccgtgc agcccgaggc cgtggtcgac ccgaccggct gcggcgatgc
     6841 gtggcgtggc gcgttgctgt acgggctgga gcagggctgg ccgctggcgc gctgcgctgc
     6901 gctgggcaac cgcttgggcg cgctcaagat cgccagccgc ggcccgcaga actaccgcgt
     6961 ggcgccgtcg atcctgaccg atctttaaac aaaaacaggc gccggcgctt gtccatcaag
     7021 cgcttccagc taccgatttc atagcgtcac agccgcgcgc gagtcaaaaa aagaagcccg
     7081 gacacgaagc atcgtgccgg gctgcgagaa ccccggctgc ttgggccggg gtgaatgcgt
     7141 cgatcagggc ttggccgcgg tcgggaaagg ccaggccgcc tgcgggttca gcgtggtttg
     7201 cgctggaggg gcaggcttgc gggcaggcgc tttcttggca gcggccttct ttgccggggc
     7261 tgcttttttc gccggggccg ctttcttggc aggcgctttc ttcgcgacgg ctttcttggc
     7321 cggtgccgct ttcttggctg ctaccttttt cgccggggct ttcttggcga cggctttctt
     7381 ggctggcgcc gctttcttcg cgggggcctt cttcgccatc acttttttcg ccggggcttt
     7441 cttggcggcg gctttcttgg ccggtgccgc tttcttcgcc ggcgccttct tggccacgac
     7501 tttcttcgct aagacctttt tggccggcgc cttcttggcc acgactttct tggccggtgc
     7561 cgctttcttg gccgctactt tgttcgcggg tgccttcttg gccggagcct tctttgccgc
     7621 agctttttta gccggtgctt tttttgcagt tgccattttt ttctccttga tcaagttgaa
     7681 ctaactatca acagcaggaa acgcttcacg cctgttggac gcgagaagcg atccatggcg
     7741 atggagagag accccatcgc catgaacacc gaggcagccc gcgcgccgct gccgggcgct
     7801 caagaagggc tgcgaaacaa gcacgggctc tagtcccacg acagcgcacc accggattgg
     7861 tactcgatga cacgcgtttc aaagaaattg cgttctttct tcaggtcgat catctcgctc
     7921 atccagggga acggattctc ttcattggga aagagcgcct ccaggccgat ctgcgtggcg
     7981 cgccggttgg cgatgtagcg caggtagccc ttgaacatgg acgcattcat gccgagcacg
     8041 ccgcgcggca tggtgtcttc ggcatagcgg tattccaact cgaccgcacg ttcgaacaag
     8101 cccttgagct cggccttgaa cgccggcgtc cacaggtgcg gattctccag cttgatctgg
     8161 ttgatcagat cgatgccgaa gttgcagtgc atcgactcgt cgc
//
LOCUS       DAOMVO010000088         8168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2285_length_8168_cov_11.960557, whole genome shotgun sequence.
ACCESSION   DAOMVO010000088 DAOMVO010000000
VERSION     DAOMVO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_8168_cov_11.960557"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKV30_05985"
     CDS             complement(<1..656)
                     /locus_tag="PKV30_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRL29802.1"
                     /translation="MRATLLRLMWLALGLGAAAPALAVPSFGDVRADFRPSETRLLDR
                     HGEPLQRLRSDAHARRGDWVRLADISPALRQALVLSEDKRFYEHSGVDWRAVSNAAWA
                     NLWHTRTRGASTLTMQLAGLLDDDLALGTGGRTLRQKIGQAVMATRLEASWRKDQILE
                     AYLNLVPFRGELVGIDALSRTLFAKAPHGLQDNEAAIAAALVRAPNARPALVAERACG
                     V"
     gene            770..1411
                     /locus_tag="PKV30_05990"
     CDS             770..1411
                     /locus_tag="PKV30_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRL29803.1"
                     /translation="MWRWFRNKAGAHPLAQPGGEIADEAEAGIDTLAEPRVWAPTVPM
                     SVPAVLDEAANRRESELRRLAALQARGELAYQYVVETAARICQTPIAAIALLDGDTLW
                     FKASVGISAETTLASHSPCLMVIEHSPAVTQIPDLRCEARFAGYPLLAAHPTTYYYAG
                     APLITSQGVPVGAVSVADDRPRELSPVQLRTLELLARQTALLLEARAPGQAPP"
     gene            complement(1603..1968)
                     /locus_tag="PKV30_05995"
     CDS             complement(1603..1968)
                     /locus_tag="PKV30_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRL29804.1"
                     /translation="MTRPTLIATLTLAALFALPAAAQHAGHAMPAAAASSPSTAAYQA
                     ANDKMHQGMTMTYTGNPDRDFLAGMIPHHQGAIDMAEVELKHGKDPKVRKLAQDIIKA
                     QKREIAQMQAWLKQMDAKR"
     gene            complement(2082..8042)
                     /locus_tag="PKV30_06000"
     CDS             complement(2082..8042)
                     /locus_tag="PKV30_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRL29805.1"
                     /translation="MSTRRTARHLAPLLCCLAAPSAHALGVTSLSPQGEVSQVRQVVA
                     KFNEDAIRFGDPKAPAPFNVQCDNASATKGQARWTSAREWVFDFADDLPPGVRCQITP
                     ISGYKSPAGAGLTSAGSYQFNTGGPFVQRIRPSPSQHIEEEQAFALMLNGDATPESVA
                     ANVWCAVDGLGERVPIRPITGEAQAALFKALGWEDAAKRAPGRYATFQCARRLTPASR
                     VQVVYGAGVATPSGVPSRIERRFGFTVREPFTASTSCERENAQAGCLPIRPIELKFSA
                     PVPRRLAELARLVGDTGEHAPIIDESTEADALIERLQFPHPLPERSAFTLRLPPDLKD
                     ASGRPLANASSFPLKIATGPMPPLAKFAAAPFGIVERFAEGPQGPALLPVTLRYVEPV
                     LKAEALHIGHLQPKSDADIIAWFTRVQRYDESLVSRKQAARDVKQPLPPSVSPETKDM
                     VETRTVSLLKGQPSVQNIELPPPPGGGERPFEVIGIPLPAGFHVLEIASQRLGQSLLN
                     DKYGAQRTMVVRTSALVTNLGVHFKLGRDNSVAWVTTLDTGKPVAGATVQVSDCHGKP
                     VASASTDARGIASFKGISPEAPRCGGGDDENFYADFRNAYFVSARADNQGAQDMAFTW
                     SSWHRGIEPWRFNVPTSNDPRPDQRAHTVFDRVLLRAGETVSMKHLLRTETAAGFGLP
                     PSGPATLVITHVGSGQEFTQPVQWRNTATGGRSAESTFAIPPAAKLGVYQVALRVPAR
                     AGEGVSGEQMLETGQFRVEEFRLPVLKGSVAPQSKDPLIAAASVPVQVQIGYVAGGPA
                     ANLPVRVSALTRNKSLRFNGYDEFSFEPPRPRAEGPQTGEDGEEESTARQDQRVVADK
                     LPVTLDRQGQGSVTIDQLKPSAGARELVVEANFADPNGEIQTLRGTAPLWPAGVIAGI
                     RTESWVSASQSAKLQALALDLNGQPKAGVPLDVKAIARITTTSRKRMVGGFYTYDNRT
                     ETKDLGTVCTGTSDTRGLLVCDVKMTQPGEVELVVSARDAQGKSAVAASSVWVTRQGE
                     IWFGGENHDRMDLLPEKKQYAAGDTARFQVRMPFRHATALVAVEREGVIETQVVELRG
                     DDPTISLKVADNWSPNVYVSVLALRGRLREVPWYSFFTWGYKSPREWWNAFWHDSKDY
                     VAPTPLVDLSKPAFRLGMAEIRVGTEAHRIDVAVKADKASYPVRGTARVSIEGKLPNG
                     QPAAGAEVAVAAVDEALLELLPNRSWDLLEAMLHRRAWGVQTATAQMEIVGRRHYGRK
                     AVPAGGDGGGAPTRELLDTLLLWQPKLQLDAQGRASIDVPLNDALTSFRIVAIADSGT
                     GLFGTGRTSIAVTQDLQIISGLPPLVRGGDAFRALIAVRNTTQAPMKVQLTPRATLLT
                     LDAQTVDVPAGEAREVTWQVTAPPQLAALRPEALLWEIEAKDLTSGARDALKVSQRVL
                     PAVPVTVQQATLVQLDGPLTQDVAAPADALPGRGGVRLSLQSKLADGLPGVRDWFARY
                     PYSCLEQKTSVAIGLRDEKLWQQTAAQLPAYLDADGLAYYFPPRAGDERRGSDILTAA
                     LLSATQEAARLNPAFAIPADSRARMIAGLTQFVEGRIERKHWSPQSDLDVRKVAALEA
                     LSRHGATRAALLSSITVAPNQWPTSAVIDWINVLKRVQGLPDQRTKLQEAEQVLRARL
                     SYQGTRMVFSTETTDHWWWLMAGGDVNSARLLLTMLDDPAWKDDLGRLASGFIARQRG
                     GAWDTTAANLWGGLALEQFSKTRESEPVSGATQAVLGDARAQVDWTKVTRLKAGDTAP
                     SVYGAPAQPGQFTGNSMLLPWPAKAVDKQPLIVTHSGSGKPWLTLQSLAAVELKAPFA
                     AGYQIKRTVTPVEQADKSLPAGQYSRGDVLRVTLDITGAADMTWVAVSDPVPGGATIL
                     GSGLGRDSAIATLGEKIGGGVQPIYEERSFEAFRAYYQYLPKGNAQIEYTVRLNNPGT
                     FQLPPTRVEALYAPEMFGESPNARVSIK"
BASE COUNT         1135 a         2693 c         2962 g         1378 t
ORIGIN      
        1 acgccgcagg cgcgttcggc caccaaggcg gggcgcgcat tgggcgcgcg caccagggcg
       61 gcggcgatgg ccgcctcgtt gtcctgcagg ccgtgcggcg ccttggcaaa cagcgtgcgg
      121 ctgagcgcgt cgatgccgac cagctcgccg cgaaacggca ccaggttgag gtaggcctcc
      181 aggatctgat ccttgcgcca gcttgcttcg agccgcgtgg ccatcacggc ctggccgatc
      241 ttctggcgca gcgtgcgccc gccggtgccc agcgccaggt cgtcgtccag caggccggcc
      301 agctgcatgg tcagagtgct ggcgccgcgc gtgcgggtgt gccacaggtt ggcccaggcg
      361 gcgttgctca cggcgcgcca gtcaacgcct gagtgttcgt aaaagcgctt gtcctccgac
      421 aacaccagcg cctggcgcag cgcgggcgag atgtcggcca gccgcaccca gtcgccgcgg
      481 cgcgcgtgcg catcgctgcg caggcgctgc agcggctcgc cgtggcgatc gagcagccgc
      541 gtttcggacg ggcggaaatc ggcgcgcaca tcaccgaagc tcggcacggc cagcgcgggc
      601 gccgccgcgc ccaggcccag cgccagccac atcagccgta acagggttgc ccgcatgcgg
      661 gcgattgtgg cagcgccgcc gggcagagaa aaaagaccgc gccttgcatg gcttctgggc
      721 aataattcac ggcatcaaca aaaagttaca tccagggcag gtcagcaaca tgtggcgctg
      781 gttccgcaac aaggcagggg ctcatccgct ggcgcagcct ggtggcgaga ttgccgacga
      841 ggccgaagcc ggcatcgaca ccctggccga gccgcgcgtg tgggcgccca ccgtgcccat
      901 gtccgtgccg gccgtgctgg acgaagcggc caaccggcgc gaatccgaac tgcggcgcct
      961 ggccgcgctg caggcgcggg gagaattggc ctatcagtac gtggtggaga ccgcagcgcg
     1021 tatctgccag acgccgatcg ccgccattgc gctgctcgat ggcgacacgc tgtggttcaa
     1081 ggccagcgtg ggcatcagcg ccgagaccac gctggcctcg cattcaccgt gcctgatggt
     1141 gatcgagcac agcccggccg tcacgcaaat acccgatctg cggtgcgagg cgcgctttgc
     1201 cggctacccg ctgctggcag cgcacccgac gacctactac tacgccggcg cgccgctcat
     1261 cacctcgcaa ggcgtgccgg tgggcgcggt gagcgtggcc gacgaccgcc cgcgcgagct
     1321 gtcgccggtg cagttgcgca cgctggagtt gctggcgcgg cagacggcgc tgttgctgga
     1381 agccagagca cctggtcaag ctcccccctg agtcggcctg cgcccgcctt cccccagggg
     1441 gacaacgctg gcgctcgggg agacccggag cgcggcgttc cggcatggcc tgcttcgcgg
     1501 ccttttgaaa gggcttggca agcgcagccc tgaacggggt tatcgaatca gaatggccgc
     1561 cagcgcttgt gcatccagcg cgagcagctc tgttttttgt agtcagcgct tggcgtccat
     1621 ctgcttgagc caggcctgca tctgcgcgat ttcgcgcttt tgcgccttga tgatgtcttg
     1681 cgccagcttg cgcactttgg ggtctttgcc gtgcttgagt tcgacctcag ccatgtcgat
     1741 ggcgccctgg tggtgcggaa tcatgccggc cagaaagtcg cggtcggggt tgccggtgta
     1801 ggtcatggtc atgccctggt gcatcttgtc gttggcggct tgatacgcgg ccgtggaggg
     1861 cgacgacgca gcggcggcgg gcatggcgtg gccggcgtgt tgcgcggcgg cgggcagggc
     1921 gaacagggcg gccagcgtca gcgtggcgat cagggtgggg cgggtcatgg tggctccttt
     1981 ctttgggtgc aaacgtgggt caggtggtgg attttgggtg tttcaggccg ccagcgcttg
     2041 ccagaaaagc gcaagcagct atcaaaaaca aagtgacacc gtcatttgat cgacacccgc
     2101 gcgttgggcg actcgccaaa catctcgggc gcgtacagcg cttcgacgcg cgtgggtggc
     2161 agctggaagg tgccggggtt gttcagccgc acggtgtatt cgatctgcgc gttgcctttg
     2221 ggcaggtatt ggtaataggc gcggaaggct tcaaagctgc gctcttcata gatcggctgc
     2281 acgccgccgc cgatcttttc gcccagcgtg gcgatggccg aatcgcgccc caggccgctg
     2341 cccagaatgg tggcgccgcc gggcaccgga tcgctgacgg ccacccaggt catgtcggcc
     2401 gcgccggtga tgtcgagcgt gacgcgcagc acgtcgccgc gcgaatactg gccggcgggc
     2461 aggctcttgt cggcttgctc aacgggcgtg acggtgcgct tgatctggta gccggcggca
     2521 aacggcgcct tcagctccac cgcggccagc gactgcagcg tgagccaggg cttgccgctg
     2581 ccgctgtggg tgacgatgag cggctgcttg tcgaccgcct tggccggcca cggcagcagc
     2641 atgctgttgc cggtgaactg accgggctgc gcgggcgcgc cgtagacgga cggtgcggta
     2701 tcgccggcct tcaggcgcgt caccttggtc caatcgacct gcgcgcgcgc atcgcccagc
     2761 acggcctggg tggcgccgct gacaggctcg ctctcgcgtg tcttgctgaa ttgctccagc
     2821 gccaggccgc cccacaggtt ggcggcggtg gtgtcccagg cgccgccgcg ctggcgggcg
     2881 atgaagccgc tggccaggcg gcccaggtcg tccttccagg ccgggtcgtc cagcatggtg
     2941 agcagcaggc gcgcgctgtt gacgtcgcca ccggccatca gccaccacca gtggtcggtg
     3001 gtttcggtgc tgaagaccat gcgcgtgccc tggtacgaca gccgcgcacg cagcacctgc
     3061 tctgcttctt gtagcttggt gcgctggtct ggcaagccct gcacgcgttt gagcacgttg
     3121 atccagtcga tcacggcgct cgtcggccac tgattcggcg ccaccgtgat gctgctcagc
     3181 agggcggcgc gcgtggcgcc gtggcgcgac agcgcttcga gcgctgcgac cttgcgcacg
     3241 tccaagtcgc tttgcggcga ccagtgcttg cgctcgatgc ggccttcaac aaactgagtc
     3301 agcccggcga tcatgcgcgc gcgcgaatcg gcggggatgg caaacgccgg gttgaggcgc
     3361 gccgcttctt gcgtggcaga gagcagagcg gccgtcagga tgtcgctgcc gcgccgctca
     3421 tcgcccgcgc gcggcgggaa gtaataggcc aggccatcgg catccagata ggccggcagt
     3481 tgcgcggcgg tttgctgcca cagcttttcg tcgcgcaggc cgatggcgac gctggtcttt
     3541 tgctccaggc acgaataagg gtagcgcgca aaccagtcgc gcacgccggg caggccatcg
     3601 gccagcttgg attgcagcga caggcgcacg ccgccgcggc cgggcagggc gtcggcgggg
     3661 gccgccacgt cttgcgtcaa ggggccatcc aactgcacca gcgtggcttg ctgcaccgtg
     3721 accggcacgg cgggcagcac gcgctggctc accttcagcg catcgcgcgc gccgctggtc
     3781 aggtccttgg cttcgatctc ccacagcaac gcttctgggc gcagcgcggc cagttgcggc
     3841 ggcgcggtga cttgccaggt gacttcgcgc gcctcgccgg cgggcacgtc gacggtttgc
     3901 gcgtcgagcg tgagcagcgt ggcgcgcggc gtcaactgca ccttcatcgg cgcctgggtg
     3961 gtgttgcgca ccgcgatcag cgcgcggaag gcgtcgccgc cgcgcaccag cggcggcagg
     4021 ccgctgatga tctgcaagtc ctgcgtgacg gcgatgctgg tgcggccggt gccgaacagg
     4081 ccggtgccgc tgtcggcgat ggcgacgatg cgaaagctgg tgagcgcgtc gttcaacggt
     4141 acgtcgatgc tcgcgcggcc ttgggcatcg agctgcagtt tgggttgcca cagcagcagc
     4201 gtgtcgagca gctcgcgcgt gggcgcgccg ccgccgtcac cgcccgccgg caccgccttg
     4261 cggccgtagt ggcggcggcc gacgatttcc atctgcgcgg tggccgtctg cacgccccaa
     4321 gcgcggcggt gcagcatggc ttcgagcaag tcccagctgc ggttgggcag cagttccagc
     4381 agtgcctcgt cgacggcggc gacggcgact tcggcgcccg cggcgggttg gccattgggc
     4441 agcttgcctt cgatgctgac gcgcgccgtg ccgcgcaccg ggtagctggc cttgtcggcc
     4501 ttcaccgcga cgtcgatgcg gtgcgcctcg gtgccgacgc ggatctcggc catgcccagc
     4561 cggaacgcgg gcttgctcaa atcgaccagc ggcgtcggcg cgacgtagtc cttgctgtcg
     4621 tgccagaagg cgttccacca ctcgcgcggg ctcttgtagc cccaggtgaa aaagctgtac
     4681 cacggcactt cgcgcagccg cccgcgcagt gccagcacgc tgacgtagac gttgggcgac
     4741 cagttgtcgg ccactttgag cgaaatcgtc ggatcgtcgc cgcgcagctc gaccacctgc
     4801 gtctcgatca cgccttcgcg ctcgacggcg accagcgccg tggcatggcg gaagggcatg
     4861 cgcacctgaa agcgcgcggt gtcgccggcg gcgtactgct ttttctcggg cagcaaatcc
     4921 atgcggtcgt ggttctcgcc gccaaaccag atttcgccct ggcgcgtcac ccacacgctg
     4981 ctggcggcca cggcgctctt gccctgggcg tcgcgcgcgc tcaccaccag ttcgacctcg
     5041 cccggctgcg tcatcttgac gtcgcacacc agcaggccgc gcgtgtcgct ggtgccggtg
     5101 cagaccgtgc ccaggtcctt ggtttcggtg cggttgtcgt aggtgtaaaa gccgcccacc
     5161 atgcgcttgc ggctggtggt ggtgatgcgg gcgatggcct tgacatcgag cggcacgccg
     5221 gccttgggct ggccgttcaa atccagcgcc agcgcctgca gcttggccga ctggctggcc
     5281 gagacccagc tttcggtgcg gatgccggca atcacgcccg ccggccacag cggcgcggtg
     5341 ccgcgcaggg tctggatctc gccgttgggg tcggcaaagt tggcctcgac caccagctcg
     5401 cgcgcgccgg cggagggctt gagctggtcg atggtcacgc tgccctggcc ttgccggtcg
     5461 agcgtgacgg gcagcttgtc ggccaccacg cgctggtctt ggcgggcggt gctttcttct
     5521 tcgccgtctt cgcccgtttg cggcccttcg gcgcgcgggc gcggcggctc aaagctgaac
     5581 tcgtcatagc cgttaaagcg cagtgacttg ttgcgcgtga gcgccgacac gcgcaccggc
     5641 aggttggccg ccgggccgcc ggccacgtag ccgatctgca cctgcaccgg cacgctggcg
     5701 gcggcgatca gcgggtcttt cgattgcggc gccacgctgc ccttgagcac cggcaggcgg
     5761 aattcttcga cgcggaactg gccggtttcc agcatctgtt cgccagacac gccttcgccg
     5821 gcgcgtgccg gcacgcgcag cgccacctgg tacacgccca gcttggcggc gggcgggatg
     5881 gcaaacgtgc tctcggcgct gcgcccgccg gtggcggtgt tgcgccactg cacgggctgc
     5941 gtgaactctt gcccgctgcc gacgtgggtg atgaccagcg tcgccgggcc gctgggcggc
     6001 aggccaaagc cggcggcggt ttcggtgcgc agcaggtgct tcatcgagac cgtttcgccg
     6061 gcgcgcagca gcacgcggtc gaacacggtg tgggcacgct ggtcgggccg cgggtcgttg
     6121 ctggtcggca cgttgaagcg ccacggctca atgccgcggt gccacgacga ccaggtgaag
     6181 gccatgtctt gcgcgccctg gttgtcggcg cgcgcgctga cgaaataggc gttgcggaag
     6241 tcggcgtaga agttctcgtc gtcaccgccg ccgcagcgcg gcgcctcggg cgaaatgccc
     6301 ttgaagctgg caatgccgcg cgcgtcggtg ctggcgctgg ccaccggctt gccgtggcag
     6361 tccgacacct gcaccgtggc gccggcgacc ggcttgccgg tgtcgagcgt ggtgacccag
     6421 gccaccgagt tgtcgcgccc cagcttgaag tgcacgccca ggttggtgac caacgcggag
     6481 gtgcgcacca ccatcgtccg ctgcgcgccg tacttgtcgt tcaacagcga ctggcccagc
     6541 cgttgcgagg cgatttccag cacgtgaaag cccgccggca gcggaatgcc gatcacctcg
     6601 aaaggccgtt cgccgccgcc gggtggcggg ggcagctcga tgttctgcac cgacggctgg
     6661 cccttcagca gcgagacggt gcgtgtttcg accatgtcct tggtctcggg gctgacgctg
     6721 ggcggcagcg gctgtttcac atcgcgcgcc gcttgcttgc gcgagaccag cgattcgtcg
     6781 tagcgctgca cgcgcgtgaa ccaggcaatg atgtcggcgt cgctcttggg ctgcaggtgg
     6841 ccgatgtgga gcgcctcggc cttcagcacc ggctcgacat agcgcagcgt gacgggcagc
     6901 agcgccgggc cttgcgggcc ttcggcaaag cgctcgacga tgccgaacgg cgcggcggca
     6961 aacttggcca gcggcggcat cggcccggtg gcgatcttga gcggaaagct gctcgcgttg
     7021 gccagcgggc ggccactggc gtccttcaaa tcgggcggca ggcgcagcgt gaaggcgctg
     7081 cgctcgggca gcgggtgggg gaactgcagg cgctcgatga gcgcatcggc ctcggtgctc
     7141 tcgtcgatga tcggcgcgtg ctcgcccgtg tcgccgacca ggcgcgccaa ctcggccagg
     7201 cggcgcggca cgggggcgct gaacttcaac tcgatagggc ggatcggcaa gcagccggcc
     7261 tgcgcgttct cgcgctcgca gctggtgctg gcggtgaacg gctcgcgcac ggtgaagcca
     7321 aagcgccgct cgatgcggct gggcacgccg ctgggcgtgg ccacgccggc gccgtacacc
     7381 acctgcacgc ggctggcggg cgtgaggcgg cgcgcacatt gaaacgtggc gtagcgcccg
     7441 ggcgcgcgct tggccgcatc ttcccagccc aatgctttga acagcgcggc ctgcgcctcg
     7501 ccggtgatgg ggcgaatcgg cacgcgctcg cccaggccat cgaccgcgca ccatacgttg
     7561 gcggccacgc tctcgggcgt ggcgtcgccg ttcagcatca gggcgaaggc ctgctcttcc
     7621 tcgatgtgct gcgaggggct ggggcggatg cgctgcacga acgggccgcc ggtattgaat
     7681 tgatagctgc ccgcgcttgt caggcctgcg ccagcgggcg atttatatcc tgaaatcggc
     7741 gtgatctgac agcgcacgcc gggcggcagg tcgtcggcaa agtcgaacac ccattcgcgt
     7801 gccgacgtcc agcgcgcctg gcctttggtg gcgctggcgt tgtcgcactg gacgttgaag
     7861 ggcgcggggg ctttcgggtc gccaaagcgg atcgcgtctt cgttgaactt ggcgacgacc
     7921 tggcgcactt gcgagacctc gccttgcggg ctgagcgagg tgacgccgag cgcgtgggcg
     7981 ctgggggcgg cgaggcagca gagcaggggg gcgaggtggc gcgcggtgcg gcgtgtggac
     8041 atgaggcgtt tgggtgatcg cggctaaagg gggagggtag caaatgagtg tggctgcgct
     8101 ttttgtgggt ggaggcgctg tcggccggga gttcgccccg gcgggcgacc tacttttctt
     8161 tgctccgc
//
LOCUS       DAOMVO010000089         8161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2290_length_8161_cov_14.667654, whole genome shotgun sequence.
ACCESSION   DAOMVO010000089 DAOMVO010000000
VERSION     DAOMVO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_8161_cov_14.667654"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKV30_06005"
     CDS             complement(<1..586)
                     /locus_tag="PKV30_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRL29806.1"
                     /translation="MNNVAKEGLEEALSRQLRSTRERLGAMLGRQGEALSPWVLRRTL
                     RELQNVADPAVSDVEGGRRAAGVAGWYGRATPEQRRDCWLLMSDQFPPDPSAIETARA
                     AYDAARGTDEEARAEVQLRKAFASPRTRLLQRFAAFPEGVRFLVDLRAELLPHLKHEK
                     RLLALDAELEDLFSTWFDVGFLELQRISWHSPAAL"
     gene            complement(629..1450)
                     /gene="lgt"
                     /locus_tag="PKV30_06010"
     CDS             complement(629..1450)
                     /gene="lgt"
                     /locus_tag="PKV30_06010"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRL29807.1"
                     /translation="MLMHPQIDPIAIKLGPLAIHWYGLTYLVAFALFFFLATRRLRHE
                     PYASITQPKPWTRQDIEDLLFWGVLGVIAGGRIGYCLFYKPAYYAAHPLEIFAIWQGG
                     MSFHGGMLGVIVAMALWAHRRERPWLQVTDLIAPCVPLGLASGRVGNFINGELWGRVA
                     DPSLPWGMVFRGAGDAPRHPSQIYQFLMEGVLLFILLWLYARKPRQMGQVSGAFLTGY
                     GLFRFIAEYFREPDAHLGLLALGMSMGQWLCIPMIVAGVLMWVWATRRQPPAPGF"
     gene            1550..3250
                     /gene="ilvD"
                     /locus_tag="PKV30_06015"
     CDS             1550..3250
                     /gene="ilvD"
                     /locus_tag="PKV30_06015"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRL29808.1"
                     /translation="MNTSKRLLNERSAHITQGKSRAPNRAMYYAMGYQEADFQKPMIG
                     VANGHSTITPCNSGLQKLADAAIAGIEEAGGNAQVFGTPTISDGMSMGTEGMKYSLVS
                     REVISDCIETCVQGQWMDGVVVIGGCDKNMPGGMMGMLRANVPSIYVYGGTILPGRLH
                     GKDINLVTVFEAVGQNAAGNMPDAELKEIERHAVPGTGSCGGMYTANTMSSAFEALGM
                     SLPYSSTMANPHDEKQNSAKESAKVLMDAIAKDLKPRDIVTKEAIENAVAVIMAIGGS
                     TNAVLHFLAIAHAAEVDWSIDDFERIRKKVPVLCDLKPSGKYMAVDLHKAGGIPQVMK
                     MLLKAGLIHGDCMTITGKTVAENLKDVPDAPPAGQDVIRPMDAPMYAQGHLAILKGNL
                     SPEGCVAKITGLKNPVITGPARVFDDEQSALQAILDGKIVAGDVMVLRYLGPKGGPGM
                     PEMLAPTGALIGAGLGESVGLITDGRFSGGTWGMVVGHVAPEAAAGGNIALIEEGDSI
                     TIDAKQLLLELHVDEATLAKRRAAWKAPAPRYTRGVQAKFAHNASSASKGAVLDDFCA
                     "
     gene            complement(3368..3622)
                     /locus_tag="PKV30_06020"
     CDS             complement(3368..3622)
                     /locus_tag="PKV30_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HRL29809.1"
                     /translation="MYLLGIGIVLLLMKWQEIGPVAAWSWWIVLAPFAAAALWWTWAD
                     WSGYTKRKAMEAEDQRKLDRINRHREAMGQKAKTAPPRRR"
     gene            3731..4042
                     /locus_tag="PKV30_06025"
     CDS             3731..4042
                     /locus_tag="PKV30_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL29810.1"
                     /translation="MKRVLLTFAALAAVTMAAPAMADEALAKSKNCMACHAVDKKLVG
                     PSYKDVAAKFAKDPGAVDMLAGKIMKGSSGVWGAVPMPPNANVSEAEAKKLAAWVMSL
                     K"
     gene            complement(4214..6196)
                     /gene="acs"
                     /locus_tag="PKV30_06030"
     CDS             complement(4214..6196)
                     /gene="acs"
                     /locus_tag="PKV30_06030"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRL29811.1"
                     /translation="MSTIESVLVENRVFPPSEATVKAARISGMDAYKALCDEAERDFD
                     GFWSRLAKENVVWSKPPTQVLDESNKPFYKWFADGQLNASANCLDKHMGTPVENKTAI
                     IFEADGGEVTKVTYRELLTRVSQFANALKSHGIQKGDRVLVYMPMTIEGIVAMQACAR
                     IGATHSVVFGGFSAKAVQERIQDGGAVAVITANYQMRGGKELPLKAIVDEALGMGGCE
                     SIKNVFVYERTPSACNMVAGRDKTFSQILEGQSTECPPEMVDAEHPLFVLYTSGSTGK
                     PKGVQHSTGGYLLWAKLTMDWTFDLKPEDIFWCTADIGWVTGHSYIVYGPLAAGATEI
                     VFEGVPTYPNAGRFWQMIERHKCSIFYTAPTAIRSLIKASEADEKVHPKSSDLSSLRI
                     LGSVGEPINPEAWMWYYKNVGGERCPIVDTFWQTETGGHMINPLPGATPLVPGSCTLP
                     LPGIQAAIVDESGNEMPNGSGGILVIKKPWPSMIRTIWNDPERFKKSYFPEELKGYYL
                     AGDGAVRDAETGYFRITGRIDDVLNVSGHRMGTMEIESALVSKTDLVAEAAVVGRPDD
                     TTGEAICAFVVLKRPLPQGDEAKQIAKELRDWVAKEIGPIAKPKDIRFGENLPKTRSG
                     KIMRRLLRSLAKGEQITQDTTTLENPAILTQLDQTY"
     gene            6379..6984
                     /locus_tag="PKV30_06035"
     CDS             6379..6984
                     /locus_tag="PKV30_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRL29812.1"
                     /translation="MSDPVQAPINRPLRPLPRLIFASRWLQLPLYLGLILAQAVYVVH
                     FIVELEHLIAAAFGSQTALQALVTSIGYKSDVPITALNETVIMLVVLALIDVVMISNL
                     LIMVIVGGYETFVSRLRLEGHEDQPEWLDHVNASVLKVKLALSIIGISSIHLLKTFIN
                     AGNYTDKVLIAQTLIHITFLFSAMAIAYTDKLMSAGEAHRH"
     gene            complement(7044..>8161)
                     /locus_tag="PKV30_06040"
     CDS             complement(7044..>8161)
                     /locus_tag="PKV30_06040"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRL29813.1"
                     /translation="GTQTNMNLNEVIANRASELLGGERGEARLVHPNDDVNKSQSSND
                     VFPTAIHVAAVEALTNHLLPAIAKLRATLAAKSRDFKDIVKIGRTHLQDATPLTLGQE
                     FSGYVAQLDHAERHVRGALPHLCELALGGTAVGTGLNAPKGYAEAAAAELARLTKLPF
                     VTAPNKFEALASCDALVYAHGTLKGLAASLMKIANDVRWLASGPRSGIGEISIPENEP
                     GSSIMPGKVNPTQSEALTMLCAQVMGNDVAINIGGASGNFELNVFRPMIAHNFLQSVR
                     LLADGMTTFNDHCAVGIEPNRARIDELVQRSLMLVTALNPHIGYDKAAQIAKKAHKEG
                     TSLREAALASGHVSGEQFDAWVVPATMTGESLSAIDK"
BASE COUNT         1412 a         2673 c         2631 g         1445 t
ORIGIN      
        1 ccagcgcggc gggcgaatgc cagctgatgc gctgcagctc cagaaaaccg acgtcaaacc
       61 aggtggagaa caaatcttcc agctcggcat cgagcgccag caggcgcttt tcgtgcttca
      121 gatgcggcaa caactcggcc cggagatcga ccagaaagcg cacgccttcg gggaaggcgg
      181 caaagcgctg cagcagccgc gtgcgcggcg aggcaaaggc cttgcgcaac tgcacctcgg
      241 cgcgcgcttc ttcgtcggtg ccgcgcgcgg cgtcgtaggc ggcgcgcgcg gtctcgatgg
      301 ccgacgggtc gggcggaaac tgatcgctca tcagcagcca gcaatcgcgc cgctgctcgg
      361 gcgtggcgcg gccgtaccag ccggccacgc cggcggcgcg gcggccgccc tcgacatcgc
      421 tgaccgccgg gtcggccacg ttctgcaact cgcgcagcgt gcgccgcaac acccagggcg
      481 agagcgcctc gccctgccgc cccaacatgg cgcccagacg ctcgcgcgtg ctgcgcagct
      541 ggcgcgacag cgcttcttcc agaccttctt ttgctacatt attcatagct gcttgcgctt
      601 gtcagtcggg cgctggagcc ctgaaacatc aaaatccggg cgcgggtggc tggcgccgtg
      661 tggcccacac ccacatcagc acgcccgcca cgatcatcgg aatgcacagc cactgcccca
      721 tgctcatgcc cagcgccagc aggcccaggt gcgcatcggg ctcgcggaag tattcggcga
      781 tgaagcggaa caggccgtag ccggtgagga aggcaccact cacctgcccc atctggcgcg
      841 gcttgcgcgc gtacagccac agcaggatga acaacagcac gccttccatc aagaactggt
      901 agatctgcga cgggtggcgc ggcgcatcac ccgcgccgcg aaacaccatg ccccagggca
      961 gcgacggatc ggccacgcgg ccccacagct cgccgttgat gaagttgccc acgcggcccg
     1021 acgccaggcc cagcggcacg cagggcgcaa tcaaatccgt cacctgcagc cacggacgct
     1081 cgcgccggtg cgcccacaac gccatggcga cgatcacgcc cagcatgccg ccatgaaagc
     1141 tcatgccgcc ctgccagatg gcgaaaattt ccagcgggtg cgcggcgtaa tacgccggct
     1201 tgtagaacag gcagtagccg atgcgcccgc cggcaatcac gcccagcacg ccccagaaca
     1261 gcaggtcttc gatgtcctgg cgcgtccacg gcttgggctg cgtgatcgac gcatacggct
     1321 cgtgccgcag gcgccgcgtg gccaggaaga aaaacagcgc gaaggcaacc agataggtca
     1381 gtccatacca gtggatcgcc agcggcccga gcttgatggc gatgggatcg atttgcgggt
     1441 gcatcagcat ggcgccgatt gtgcacggcc gctacggcgc ccgggtgttt gcgggtgcct
     1501 acaattccaa ggcacgcttt cccacgacaa ccgacccgga acctggccca tgaacacctc
     1561 caaacgcctt ctgaacgaac gcagcgccca catcacgcaa ggcaagagcc gcgcgcccaa
     1621 ccgcgccatg tactacgcca tgggctacca ggaggcggac tttcaaaagc ccatgatcgg
     1681 cgtcgccaac ggccacagca ccatcacgcc ctgcaacagc ggcctgcaga agctggccga
     1741 cgcggcgatt gccggcatcg aggaagccgg cggcaacgcg caggtgttcg gcacgccgac
     1801 catcagcgac ggcatgtcga tgggcaccga gggcatgaag tacagcctgg tcagccgcga
     1861 ggtgatcagc gactgcatcg agacctgcgt gcaaggccag tggatggacg gcgtggtcgt
     1921 catcggcggc tgcgacaaga acatgcccgg cggcatgatg ggcatgctgc gcgccaacgt
     1981 gccgtcgatc tacgtctatg gcggcaccat tctgccgggg cgcctgcatg gcaaggacat
     2041 caacctggtc accgtgttcg aggccgtggg ccagaacgcc gccggcaaca tgcccgatgc
     2101 cgagctgaag gagatcgagc gccacgccgt gcccggcacc ggctcgtgcg gcggcatgta
     2161 caccgccaac accatgtcgt cggccttcga ggcgctgggc atgagcctgc cctactccag
     2221 caccatggcc aacccgcacg acgagaagca gaactcggcc aaggaatcgg ccaaggtgct
     2281 gatggacgcc atcgccaagg atttgaagcc gcgcgacatc gtgaccaagg aggcgatcga
     2341 gaacgccgtg gccgtgatca tggccattgg cggctcgacc aacgcggtgt tgcacttcct
     2401 ggccatcgcg cacgcggcgg aagtggattg gtccatcgac gacttcgagc ggatccgcaa
     2461 gaaggtgccc gtgctgtgcg atctgaagcc cagcggcaag tacatggcgg tcgatctgca
     2521 caaagccggc ggcattccgc aggtgatgaa gatgctgctg aaggccggcc tgatccacgg
     2581 tgattgcatg acgatcaccg gcaagacggt ggccgaaaac ctgaaggacg tgcccgacgc
     2641 gccgcccgca ggccaggacg tgatccgccc gatggatgcg cccatgtacg cgcaaggcca
     2701 cttggcgatc ctgaagggca atctgtcgcc cgaaggctgc gtggccaaga tcaccgggct
     2761 gaaaaacccc gtcatcacgg gcccggcgcg cgtgttcgac gacgagcagt cggcgttgca
     2821 agccattctg gacggcaaga tcgtggccgg cgacgtgatg gtgctgcgct acctgggccc
     2881 caagggcggc cccggcatgc ccgagatgct ggcgccgact ggcgcgctga ttggcgccgg
     2941 tctgggcgaa agcgtgggcc tgatcaccga tgggcgcttt tctggcggca cctggggcat
     3001 ggtggtcggc cacgtggcgc ccgaggcggc ggccggcggc aacatcgcgc tgatcgagga
     3061 aggcgattcg atcaccatcg acgccaagca gctgctgctg gaactgcacg tggatgaagc
     3121 cacgctggcc aagcgccgcg ccgcctggaa ggcgcccgcc ccgcgctaca cgcgcggcgt
     3181 gcaggccaag tttgcgcaca acgcgtcgag cgcgagcaaa ggcgcggtgc tggacgattt
     3241 ctgcgcctga cggtcggaag ggcatcgaac gggtgacgtg ttcggcgtca cccgtttact
     3301 atcattaaag tagctgcccg cgcttgctca tcaagcgctg gtggccgttt tgacccttaa
     3361 ctaaccatca gcgccggcgc ggtggcgccg tcttggcctt ttgtcccatc gcctcgcggt
     3421 ggcggttgat gcggtccagc ttgcgctggt cctcggcttc catcgccttg cgcttggtgt
     3481 agcccgacca gtccgcccag gtccaccaca aggccgcggc cgcaaacggc gccagcacga
     3541 tccaccacga ccaggcggcc accggaccga tttcctgcca tttcatgagc aacaagacga
     3601 tcccgattcc cagcaagtac atggtctttc cttgatttac gccagttttt gctcttgggc
     3661 caagtcaacc ggccgcctta gaatggcgtt gaacgactgt agcgcaacta cccacgatgg
     3721 agatatgacg atgaagcgtg ttctgttgac ttttgccgcc ctggccgccg tcacgatggc
     3781 cgcacccgcc atggcggacg aagcactggc aaagtccaaa aactgcatgg cttgccatgc
     3841 agtcgacaaa aaactggtcg gcccctccta caaggacgtg gctgccaagt tcgccaagga
     3901 tcccggcgcg gtcgacatgc tggccggcaa gatcatgaag ggcagcagcg gggtgtgggg
     3961 cgcggtccca atgcccccca acgccaacgt gagcgaagcc gaagccaaga aactggccgc
     4021 ttgggtcatg agcctcaagt aatccgttcg cggcgccact ggcgcagcac aaaaaaagcc
     4081 cgcgaatcgc gggctttttt tcgtccgtca gcaggccgcg gagcaggcca tcccggaacg
     4141 ccgcgctcgg ggtctccccg agcgccagcg ttgtccccct ggggggaaga cgccgcaggc
     4201 ggctcagggg ggatcaataa gtctgatcca gctgcgtcag gatcgccgga ttctccagcg
     4261 tggtggtgtc ctgcgtgatc tgctcgccct tggccaggct gcgcaacagg cgccgcatga
     4321 tcttgcccga gcgcgtcttg ggcaggttct cgccaaagcg gatgtccttg ggtttggcga
     4381 tgggtccgat ctccttggcc acccagtcgc gcagctcctt ggcgatctgc ttggcctcgt
     4441 cgccctgcgg cagcgggcgc ttgagcacga caaacgcgca gatcgcctcg cccgtcgtgt
     4501 cgtcgggccg gcccaccacc gccgcttcgg ccaccaggtc ggtcttggag accagggccg
     4561 actcgatttc catcgtgccc atgcggtggc ccgacacgtt cagcacgtcg tcgatgcggc
     4621 cggtgatgcg gaagtagccg gtctcggcat cgcgcacggc gccgtcgccg gccaggtaat
     4681 agcctttcag ctcttcgggg aagtagctct tcttgaagcg ctcggggtcg ttccagatgg
     4741 tgcggatcat gctgggccag ggcttcttga tgaccaggat gccgcccgag ccattgggca
     4801 tctcgttgcc cgattcgtcc acgatggcgg cctggatgcc cggcagcggc agcgtgcacg
     4861 agcccggcac cagcggcgtg gcgcccggca gcgggttgat catgtggccg ccggtttcgg
     4921 tctgccagaa ggtgtcgacg atggggcaac gctcgccacc gacgttcttg tagtaccaca
     4981 tccaggcttc ggggttgatc ggctcgccca cgctgccgag gatgcgcagg ctgctcagat
     5041 ccgaactctt ggggtgcact ttttcatcgg cttccgatgc cttgatgagc gagcggatgg
     5101 cggtcggcgc ggtgtagaag atgctgcact tgtggcgctc gatcatctgc cagaagcggc
     5161 cggcgttcgg gtaggtgggc acgccctcga acacgatctc ggtggcgccg gcggccagcg
     5221 ggccgtagac gatgtagctg tggcccgtga cccagccaat gtcggccgtg caccagaaga
     5281 tgtcttcggg cttgaggtcg aacgtccagt ccatggtgag cttggcccac agcaggtagc
     5341 cgccggtgga gtgctgcacg cccttgggct tgccggtgga gccgctggtg tagagcacga
     5401 acagcggatg ctcggcatcg accatctcgg gcgggcattc cgtgctctgg ccttcgagta
     5461 tctggctgaa ggtcttgtcg cggccggcga ccatgttgca ggcgctgggc gtgcgctcgt
     5521 acacgaagac gttcttgatc gactcgcagc cgcccatgcc cagcgcctcg tcgacgatgg
     5581 ctttgagcgg cagttccttg ccgccgcgca tctggtagtt ggcggtgatc accgccaccg
     5641 cgccgccgtc ctgaatgcgc tcttgcaccg ccttggccga gaagccgcca aacaccacgc
     5701 tgtgcgtggc gccgatgcgc gcgcaggcct gcatggcgac gatgccctcg atcgtcatgg
     5761 gcatgtagac cagcacgcgg tcgcccttct ggatgccgtg gctcttgagc gcattggcga
     5821 actggctgac gcgcgtcagc aattcgcggt acgtgacctt ggtgacttcg ccgccatcgg
     5881 cctcgaagat gatggcggtc ttgttctcga ccggcgtgcc catgtgcttg tcgaggcagt
     5941 tggcactggc gttcagctgg ccgtcggcaa accacttgta gaacggcttg ttcgattcat
     6001 ccagcacctg cgtgggcggc ttggaccaga ccacgttttc cttcgccagg cgcgaccaga
     6061 agccgtcgaa atcgcgctcg gcctcgtcgc acaaggcctt gtacgcatcc atgccgctga
     6121 tgcgcgccgc cttcacggtg gcttcgctgg gcgggaacac gcggttttca accagaaccg
     6181 attcaatggt gctcatgtct cttgtctcct tttttcgttg caataccgtt aaggcgcgcg
     6241 ggtgcggcgc cgcaaacttt tggatgatcg ggcgaggctc ttacaaacct ctgactcgcg
     6301 cgactctagc gctgacgagg cgccctgccc gcgcgatgcc aggtcctaca atacgccgtt
     6361 ttttcaagca ccctcttcat gagcgacccc gtccaggccc ccatcaaccg cccgctgcgg
     6421 ccgctgcccc ggctgatttt cgccagccgc tggctccagt tgccgctgta cctgggcctg
     6481 atcctggcgc aggcggtgta cgtggtgcac ttcatcgtcg agctggagca cctgattgcc
     6541 gccgcctttg gcagccagac cgcgctgcag gcgttagtga ccagcatcgg ctacaagagc
     6601 gatgtgccca tcacggcgct gaacgaaacc gtgatcatgc tggtggtgtt ggcgctgatc
     6661 gacgtggtga tgatctccaa cctgctcatc atggtcatcg ttggcggcta cgagaccttt
     6721 gtcagccggc tgcggctgga agggcatgaa gaccagcccg aatggctcga ccacgtcaac
     6781 gccagcgtgc tgaaggtgaa gctggcgctg tcgatcatcg gcatcagctc gattcacctg
     6841 ctcaagacct tcatcaacgc cggcaactac accgacaaag tgctgatcgc gcagacgctg
     6901 atccacatca ccttcttgtt ctcggccatg gcgatcgcct acaccgacaa gctgatgtcg
     6961 gccggcgagg cgcatcgcca ttgagttact cgggtttttg aatgaaatgt gccgccagcg
     7021 cttgatggac aagcgcgaat agctcattta tcgatagcgc tcaacgattc accggtcatc
     7081 gtcgccggca ccacccaggc gtcgaactgt tccccgctca cgtgcccgct ggccagcgcg
     7141 gcttcgcgca ggctagtgcc ttccttatgc gctttcttgg cgatctgcgc cgccttgtcg
     7201 tagccgatgt gcgggttgag cgcggtcacc agcatcagcg agcgttgcac caattcgtca
     7261 atccgcgcgc ggttcggctc gatgccgaca gcgcagtggt cgttgaaggt ggtcatgcca
     7321 tcggccagca ggcgcacgct ctgcaggaag ttgtgcgcga tcatggggcg gaacacgttg
     7381 agctcgaagt tgcccgatgc gccgccgatg ttgatggcca cgtcgttgcc catgacctgc
     7441 gcgcacagca tggtcagcgc ctcgctctgc gtcgggttga ccttgccagg catgatcgat
     7501 gagcccggct cgttttcggg aatgctgatc tcgccgatgc cgctgcgcgg cccgctggcc
     7561 agccagcgca cgtcgttggc gatcttcatc aggctggcgg ccagcccttt cagcgtgccg
     7621 tgggcgtaga ccagtgcgtc gcagctggcc agcgcttcga acttgttggg cgcggtgaca
     7681 aaaggcagct tcgtcagtcg cgccagttcg gcggcggcgg cttccgcata gcccttgggc
     7741 gcgttcaagc ccgtgccgac ggccgtgccg cccagcgcca attcgcacag gtgcggcagc
     7801 gcgccgcgca cgtggcgctc ggcatggtcg agctgcgcca cgtagcctga gaattcctgc
     7861 cccagcgtca gcggcgtggc gtcttgcagg tgggtgcggc cgatcttgac gatgtccttg
     7921 aagtcgcgcg acttggcggc cagcgtggcg cgcagcttgg cgatggccgg cagcaggtgg
     7981 tttgtcaacg cctccaccgc cgccacgtgt atcgccgtgg ggaacacgtc gttgctcgac
     8041 tggctcttgt tcacgtcgtc gttggggtgc accaggcgcg cctcgccgcg ttcgccgccc
     8101 agcagttcgc tggcgcggtt ggcgatgacc tcgttgaggt tcatgttggt ctgcgtgccc
     8161 g
//
LOCUS       DAOMVO010000090         8052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2328_length_8052_cov_10.960860, whole genome shotgun sequence.
ACCESSION   DAOMVO010000090 DAOMVO010000000
VERSION     DAOMVO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_8052_cov_10.960860"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..295)
                     /locus_tag="PKV30_06045"
     CDS             complement(101..295)
                     /locus_tag="PKV30_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRL29814.1"
                     /translation="MIRWLIVVFLALVLLNGLAPWLKKLGIGRLPGDFHFKLFGREWS
                     IPLTSTLLLSAAAALISKLI"
     gene            complement(311..1384)
                     /locus_tag="PKV30_06050"
     CDS             complement(311..1384)
                     /locus_tag="PKV30_06050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRL29815.1"
                     /translation="MHLALYEPGLGYYANASRKFGALAASGSDFVTAPELSPLFGRAL
                     AAQVADSLAATGTDEVWEFGAGSGALAAQLIAELDALGQPLKRYTIVDVSGALRARQQ
                     ERLAAFAPRVAWAYRLPDALRGVVVGNEVLDAMPVPLLIRKSGIWHERGVSVFMPNQA
                     STPAQQAPAATDSEASQFTWADRPTALRPPIEVDGPHDYLTEIHPQAHAFVATLAEAL
                     RRAGAGAAFFIDYGFPEAEYYHPQRAMGTLVCHRAHQVDDDPLADVGAKDITAHVNFT
                     GVALAAQDAGLDVLGYTSQARFLLNCGIAQMMEAADLPTRANAMKLLAEHEMGELFKV
                     VGFATPNHGPARGFAQGDRSHRL"
     gene            1536..2309
                     /locus_tag="PKV30_06055"
                     /pseudo
     CDS             1536..2309
                     /locus_tag="PKV30_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            2307..2744
                     /locus_tag="PKV30_06060"
     CDS             2307..2744
                     /locus_tag="PKV30_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRL29816.1"
                     /translation="MNRAEPPDGRALAAPVLEADTVPPVEQGGRILTLTGLRFDANLG
                     ILDHEKQAPQPIQVDAELNLGGQPLRPRDDEIHHVLDYRKVRQIVIDECTAEHVNLLE
                     SLIGKLCQRLLGLPGVLGVRVKIAKLEIFDDCEVAIRMEAGRW"
     gene            2808..3758
                     /gene="ttcA"
                     /locus_tag="PKV30_06065"
     CDS             2808..3758
                     /gene="ttcA"
                     /locus_tag="PKV30_06065"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HRL29817.1"
                     /translation="MTEATVSLAEPDLDTAAGTGPSARSKAEREALKLEKRLCRQVGQ
                     AIADFNMIEAGDRVMVCLSGGKDSYGLLDVLMKLQARAPVRFELVAVNLDQKQPGFPA
                     EVLPNYLQQLGIEHHIETQDTYSIVKAKIPEGKTTCSLCSRLRRGILYSVARRLKCNK
                     IALGHHRDDMLQTFFLNMFFGGKLKGMPPKLTSDNGEFIVIRPLAYVDERDLVAWAAH
                     RAFPIIPCNLCGSQENLQRQVVGDMLREWQKKHPGRIENMFAALQNVVPSHLMDATLH
                     DFKHLQATGVPDEGGDKAFDEESFAAPVGQPFAGVTLVGA"
     gene            3827..4468
                     /locus_tag="PKV30_06070"
     CDS             3827..4468
                     /locus_tag="PKV30_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29818.1"
                     /translation="MLTACASGPRTIDAEVRTNAAQPPGHAVLQGAHYRFERGQLVAG
                     QPDPAKLEAMAQTALARVGAVRDDAQPRVSVQVGGYVSAYWMDDSYYGGLSNPRIALG
                     MGVGRGGFGFGMGWPLSDPSIPAYVSEVNLLMRDLQTGQIVYDTRARHDGPWHDTDNV
                     LAALFVAALEGYPQPAMSVRRVGVPLVPVTAAPAPALPTRGAAPMAPGQPAPR"
     gene            4542..5195
                     /locus_tag="PKV30_06075"
     CDS             4542..5195
                     /locus_tag="PKV30_06075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL29819.1"
                     /translation="MKATRILAIRHGETAWNVDGRLQGHRDIPLGDTGRVQARRLARA
                     LAGRGDIDAIVSSDLSRALETARIVAEAQGLPVTTDIGLRERCFGDFEGRTFAEVSAA
                     WPAEAERWRRRDPDWSPPGEQAESLLRFQERVTHAVQALAAQNMGKQLALFTHGGVLD
                     VLYRAATGLGLRDARTWALGNTAVNRLLWTPDGGLTLVGWADTMHLADESLDEQSAA"
     gene            5228..6406
                     /locus_tag="PKV30_06080"
     CDS             5228..6406
                     /locus_tag="PKV30_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HRL29820.1"
                     /translation="MEKPEIPHRVRALIGQSVDAIDTPALVVDLDAANRNLQRMAEFA
                     AKHRLRLRPHAKMHKSAEFARLQLQAGAIGLCAQTVAEAEALGAGGVNDVFISNEILA
                     VPKLRRVAALAHQLKSRGGQLAIAVDSFEGVERLAQAMQLTNAVIDVFIELDIGHGRC
                     GVASPDAAVQLAQLISRRSTGLRYAGLHAYHGAAQHLRSVDERRQALTDAAARLNAAR
                     DALAAAELAPPLVTGAGTGTFSIESASGLWGEIQPGSFLFMDRDYLQNERDPAQPQFE
                     PALFVKSQVISVGEKHAVCDAGHKSHAIDSGLPTVRTLPGQARLEFANGGDEHGVLKP
                     LDYGGALPTLPELGETVWLIPGHCDPTVNLHEALIVVRDGIAAGRVEAVVPVQARGVW
                     "
     gene            complement(6613..7092)
                     /locus_tag="PKV30_06085"
     CDS             complement(6613..7092)
                     /locus_tag="PKV30_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL29821.1"
                     /translation="MKPDDTDWELLATLQRDASPSNQALAEQVRISPPTALRRVRRLH
                     EEGLIERQVALLDEDRIAALQGHGLTAIVEVTLDRQGAEHLDAFERRAVQDDAVRQCY
                     RVAPGPDFVLIVRVHDMPAYQALAERLFTQDANVRNVRSFFSVKRAKFETQVPLARG"
     gene            7247..>8052
                     /locus_tag="PKV30_06090"
     CDS             7247..>8052
                     /locus_tag="PKV30_06090"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903607.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRL29822.1"
                     /translation="MCGIVGAVSTRNIVPILVQGLQRLEYRGYDSCGVAVHAADASGP
                     GTRLQRLRGIARVADLAAQAESQPLAGHTGIAHTRWATHGVPAVTNAHPHVSRGPVPG
                     GMGGDEAAARPARVTLVHNGIIENHETLRAELLARGYFFASQTDTEVIVHLIDSLYDG
                     DLLAAVQAAVRRLRGSYSLAVLHRDEPQRIVGAREGSPLVLGLGADGKENFLASDAMA
                     LAGVTDQIVYLEEGDVVDVQLAKHWIVGRAGQRVQRPVKTVQAMSGAAELG"
BASE COUNT         1287 a         2775 c         2755 g         1235 t
ORIGIN      
        1 ttcatgctcc tgataacgaa gcatcctcat aattcgatga cacgccctag cgcaatagga
       61 gaaagcgcga gcagctatcc ttttaggact ggttggcggc tcaaatcagc ttcgaaatca
      121 aggcggcagc ggcactgagc agcaacgtgc tggtcagcgg aatcgaccat tcgcgcccga
      181 acagcttgaa atggaagtcg cccggcaggc ggccgatgcc cagcttcttc agccacggcg
      241 ccaggccgtt gagcagcacc agggccagaa aaacgacgat cagccagcgg atcatggggc
      301 gggcccgcgg ttacagccgg tggctgcggt cgccctgggc gaagccgcgc gccgggccat
      361 ggttcggcgt cgcaaagccc accaccttga acagctcgcc catctcatgc tcggccagca
      421 gcttcatggc gttggcgcgc gtgggcaggt cggccgcttc catcatctgg gcgatgccgc
      481 agttcagcag aaagcgcgcc tggctggtgt agcccagcac gtcgaggccc gcgtcctgcg
      541 cggccagcgc cacgccggtg aagttgacgt gcgcggtgat gtccttggcg cccacgtcgg
      601 ccagcgggtc gtcgtccacc tggtgcgcgc ggtggcacac cagcgtgccc atggcgcgct
      661 gcgggtggta gtactcggcc tcggggaaac cgtagtcgat gaagaaggcc gcgcccgcgc
      721 cggcgcgccg cagcgcctcg gccagcgtgg cgacgaacgc gtgcgcctgc gggtggatct
      781 cggtcaggta gtcgtgtggg ccgtctacct cgatgggcgg gcgcagcgcg gtagggcggt
      841 cggcccaggt gaattgactt gcttctgaat ccgtagctgc tggcgcttgc tgggcgggcg
      901 ttgaagcctg atttggcatg aaaacgctca ctccgcgttc gtgccatata ccgcttttcc
      961 gaatcagcag cggcacgggc atggcgtcca gcacctcgtt gcccaccacc acgccgcgca
     1021 gcgcgtcggg caggcgatag gcccaggcca cgcgcggggc aaaggcggcc aggcgctcct
     1081 gctggcgcgc ccgcagcgcg cccgacacat ccacgatggt gtagcgcttc aggggctggc
     1141 ccagcgcgtc cagctcggcg atcagctgcg ccgccagcgc gcccgaaccg gcgccgaact
     1201 cccacacttc gtcggtgccc gtcgcggcca ggctgtcggc cacctgcgcc gccagcgcgc
     1261 ggccgaacag gggcgacagc tcgggcgcgg tgacgaagtc gctgcccgat gccgccagcg
     1321 cgccgaactt gcgcgacgcg ttggcgtagt agcccaggcc gggctcgtac agcgccaggt
     1381 gcatgaagcg ctcaaagccg atccagccgc ccgccgcatg aatatcggcc gcgatgcgcg
     1441 ccgccagctc gcccgacaaa gaggctggcg ccgcgccgct cgcgccgggg gcgctggtta
     1501 cactcgattc cgtttggttc accccctgat tgtccttgaa caccgctgcc ccgcttcgca
     1561 ccgtgctcgt caccggcgcc ggccgccggc tcggccgcgc catcgcgctg gcctgcgccc
     1621 aggcgggctg gcgcgtcgcc gtgcattacc accagtcgcg cgccgatgct ctggaaaccg
     1681 cagctgcctg cgcccagtgg gcgcccgctg gaggggtttt tgatgccgat ctggcggacg
     1741 aggcgcaaac ccgcgcgctg ctgccccgcg tggtggccga gctgggcgcc gtgcacgccg
     1801 tggtcaacag cgccgcgctg ttcgagtttg acgatgcggc cagcttcagc acggcgctgc
     1861 tcgaccgcca cacgcacacc aacaccgccg cgcccgtgct gctggcgcag gcgctggccg
     1921 agcacctggc cgcgcgcggc gcgccgtccg ccagcggctg cgtggtcaat ctgctggacc
     1981 agaagctgtt caaccccaac ccggattttt tgagctacac gctcagcaaa gcggcgctgc
     2041 agagcgccac cacactgctg gcgcaggcgc tggcgccgcg cgtgcgcgtg gtcggcgtgg
     2101 cgccgggcct cacgctgacc agccatttgc tgagcgacga gcgctttgcc gagctgcacc
     2161 agcaaagccc gctgggccgc tcatccacgc cggaagacgt ggcggcggcc gtggtgttcg
     2221 cgctgggcaa tgcctccatc acgggcgcca cgctgctggt cgatggcggc cagcacctga
     2281 tgcgcttcga gcgcgacttt tccatgatga atcgggctga accgcccgat gggcgggcgc
     2341 tggcagctcc tgttttggaa gcggatacag tgccgccggt cgagcagggc ggccgcattc
     2401 tgacgctgac cggcctgcgc ttcgacgcca acctgggcat cctcgaccac gaaaaacagg
     2461 cgccgcagcc cattcaggtg gatgcagaac tgaacctggg cggccagccc ctgcgcccgc
     2521 gcgacgacga gatccaccac gtgctggact accgcaaggt gcgccagatc gtgatcgacg
     2581 aatgcacggc cgagcacgtc aacctgctgg aaagcctgat cggcaagctg tgccagcgcc
     2641 tgctgggcct gcccggcgtg ctgggcgtgc gcgtgaagat cgccaagctg gagatctttg
     2701 acgactgcga agtggccatc cggatggaag ccgggcgctg gtaggccgcg gcccggcgcc
     2761 gccgggtgcg ctgcgggtga aaggcgcatc tgggacaatt gaaggctatg accgaagcca
     2821 ccgtttccct ggccgaaccc gatctggaca ccgcagcggg caccggcccg tccgcccgtt
     2881 ccaaggccga gcgcgaggcg ctcaagctgg aaaagcgcct gtgccgccag gtcgggcagg
     2941 ccattgccga cttcaacatg atcgaggcgg gcgaccgcgt gatggtgtgc ctgtcgggcg
     3001 gcaaggacag ttatggcctg ctcgacgtgc tgatgaagct gcaggcgcgc gcgccggtgc
     3061 gctttgagct ggtggcggtc aacctcgacc agaagcagcc gggctttccg gccgaggtgc
     3121 tgccgaacta cctgcaacaa ctgggcatcg agcaccacat cgagacgcag gacacctaca
     3181 gcatcgtcaa ggccaagatc cccgagggca agaccacgtg cagcctgtgc tcgcgcctgc
     3241 gccggggcat tctgtacagc gtggcgcggc ggctcaagtg caacaagatc gcgctcggcc
     3301 accaccgcga cgacatgctg cagaccttct tcctcaacat gttctttggc ggcaagctga
     3361 agggcatgcc gcccaagctg accagcgaca acggcgagtt catcgtcatc cgcccgctgg
     3421 cctacgtgga cgagcgcgac ttggtcgcct gggccgcgca ccgcgccttc cccatcatcc
     3481 cgtgcaacct gtgcggcagc caggagaacc tgcagcgcca ggtggtgggc gacatgctgc
     3541 gcgagtggca gaaaaagcac ccgggccgca tcgagaacat gtttgccgcg ctgcagaacg
     3601 tcgtgccttc gcacctgatg gacgccacgc tgcacgactt caagcacctg caagcgaccg
     3661 gcgtgcccga cgagggcggc gacaaggcgt ttgacgagga atcgtttgcc gcccctgtcg
     3721 gccaaccgtt tgccggcgtg acgctggtgg gcgcttgatt tttggatcga attcgacgga
     3781 ggcgcttatg tggcttgcgc gagcagctct tgtgttgata gcaaccgtgc tgacggcctg
     3841 cgccagcggc ccgcgcacca tcgacgccga agtgcgcacc aacgccgcgc aaccgccggg
     3901 ccacgcggtg ctgcagggcg cgcactaccg cttcgagcgc ggccagctgg tggcgggtca
     3961 gcccgatccc gccaagctcg aagccatggc gcagacggcg ctggcgcgcg tcggcgcggt
     4021 gcgtgacgat gcgcagccgc gcgtcagcgt gcaggtgggt ggttacgtca gcgcctactg
     4081 gatggacgat tcgtactacg gcgggctgag caacccgcgc atcgcgctgg gcatgggcgt
     4141 ggggcgcggc ggctttgggt tcggcatggg ctggccgctg tccgaccctt cgattcccgc
     4201 ctacgtgagc gaggtcaacc tgctcatgcg cgatctgcag accggccaga tcgtctacga
     4261 cacgcgcgcc cgccacgacg gcccctggca cgacaccgac aacgtgctgg ccgccctgtt
     4321 cgtggccgcg ctggagggct acccgcagcc cgcaatgtcg gtgcggcgcg tgggcgtgcc
     4381 gctggtgccg gtgacggctg cccccgcgcc cgcgctgccc acccgcgggg ccgcgcccat
     4441 ggcaccgggc cagccggcgc cgcgctgagg ctgttgcgcg ctgcccgctg aatgcccatt
     4501 ggggtatccg gccgcagcct gccccgtggc atgatggcgc catgaaggcc acccgcattc
     4561 ttgcgatccg ccatggcgaa accgcctgga acgtggatgg ccgcctgcag ggccaccgcg
     4621 acattccgct gggcgacacc ggccgcgtgc aggcgcggcg cctggcgcgg gcgctggcgg
     4681 ggcgcggcga catcgacgcc atcgtcagca gcgacctgag ccgcgcgctg gaaaccgcgc
     4741 gcatcgtggc cgaggcgcag ggcctgccgg tcacgaccga catcggcctg cgcgaacgct
     4801 gctttggcga tttcgagggc cgcacctttg ccgaggtcag cgccgcctgg ccggccgagg
     4861 ccgaacgctg gcgccggcgc gaccccgact ggtcgccgcc cggcgagcag gccgaatcat
     4921 tgctccgatt tcaagagcgc gtcacgcacg ccgtgcaagc gctggcggcc caaaacatgg
     4981 gcaagcaact ggcgctgttc acgcacggcg gcgtgctcga cgtgctgtac cgggccgcca
     5041 ccggcctcgg cctgcgggac gcgcgcactt gggccttggg caacaccgcc gtcaaccgcc
     5101 tgctgtggac gcccgacggc ggcctgacgc tggtcggctg ggccgacacc atgcatctgg
     5161 ccgacgagtc actggacgag caaagcgcgg cctgacgccg cgccgaaaaa aagaaagaaa
     5221 ggctgccgtg gagaaacccg agattccaca ccgggtgcgt gcactcatcg gccagagcgt
     5281 cgacgccatc gacacgccgg cgctggtggt cgacctggac gccgccaacc gcaacctgca
     5341 gcgcatggcc gaatttgccg ccaagcaccg gctacgcctg cggccgcacg ccaagatgca
     5401 caagagcgcc gaattcgcgc gcctgcagct gcaggccggc gccatcggcc tgtgcgcgca
     5461 aaccgtggcc gaggccgagg cgctgggcgc cggcggcgtc aacgacgtct tcatcagcaa
     5521 cgaaatcctg gctgtgccca agctgcgccg cgtggccgca ctggcccacc agctcaaaag
     5581 ccgcggcggc cagttggcga ttgctgtgga cagcttcgag ggcgtcgagc ggctggcgca
     5641 ggccatgcag ctgaccaacg cggtgatcga cgtcttcatc gagctcgaca tcggccacgg
     5701 ccgctgcggc gtggcatcgc ccgacgcggc ggtgcagctg gcacaactga tttcgcgccg
     5761 ttccaccggc ctgcgctacg ccggcctgca cgcctatcac ggcgcggcgc agcacctgcg
     5821 cagcgtggac gagcgccgcc aggccttgac cgatgctgcc gcgcgcctga acgccgcgcg
     5881 cgatgcgctg gcggcggccg agctggcgcc gccgctggtc accggcgccg gcaccggcac
     5941 cttttccatc gaatcggcca gcgggctgtg gggcgaaatc cagcccggca gcttcttgtt
     6001 catggaccgc gactacctgc aaaacgagcg cgacccggca cagccgcagt tcgagccggc
     6061 gctgttcgtc aagtcgcagg tcatcagcgt gggcgaaaaa cacgccgtgt gcgacgccgg
     6121 ccacaagagc cacgccatcg acagcggcct gccgacggtg cgcaccttgc caggccaggc
     6181 gcggctggaa tttgccaacg gcggcgacga acacggcgtg ctcaagccgc tcgactacgg
     6241 cggcgcgctg cccaccctgc ccgagctggg cgaaaccgtg tggctgattc ccggccactg
     6301 cgacccgacg gtgaatttgc acgaggcgct gatcgtggtg cgcgatggta ttgcggcggg
     6361 ccgggtcgag gcggtggtgc cggtgcaggc gcgcggcgtg tggtgaaggt agcttgtcca
     6421 ctcactattt gatagctttc tgcgcttgat ggacgtgcgc tagcggcctg ttctggcaaa
     6481 aaatcagacg tattgagcgc tgaaaattcg ctgaagggac atgctccctt tggtcttctt
     6541 tcgcgtcctg cgcgaaccct ttgcgtcctt cgcgtccggc tgttgggttg ttcggcaacg
     6601 ccctgaccgc gctcagcccc gcgccagcgg cacctgtgtc tcgaacttcg cccgcttgac
     6661 cgagaagaag ctgcgcacgt tgcgcacgtt ggcgtcttgg gtgaacaggc gctcggccag
     6721 tgcctgatac gccggcatgt cgtgcacgcg cacgatcagc acaaagtcag gccctggcgc
     6781 cacgcggtag cactggcgca cggcatcgtc ctgcacggcg cggcgctcga aggcatccag
     6841 gtgctcggcg ccctggcggt ccagcgtcac ctcgacgatg gccgtcaggc cgtggccttg
     6901 cagcgcggcg atgcggtctt cgtccagcag cgccacctgg cgttcgatca ggccttcctc
     6961 atgcaaccgc cgcacgcgcc gcagcgcggt ggggggcgaa atgcgcactt gctcggccag
     7021 cgcctggttg cttgggctgg cgtcgcgttg cagggtcgcc agcagctccc aatcggtgtc
     7081 gtctggtttc atgtgactcg ctattctgat agaaaaattc aagtttgttt gagtttgttg
     7141 ataaatttca tataagcacc aaatgagatg atgaaatttc gagatcgttt tcataacatc
     7201 agcgcttcgc cgcgaagcgc ggtggccagt ccaaaggaaa tctcccatgt gcggcatcgt
     7261 cggcgcggtc tccacgcgca acatcgttcc catcctggtg cagggtctgc agcggctcga
     7321 ataccgcggc tacgactcgt gcggcgtggc ggtgcacgcg gccgatgcgt ccggccccgg
     7381 cacgcgcctg cagcgcctgc gcggcatcgc tcgcgtggcc gatctggcgg cgcaggccga
     7441 gtcgcaaccg ctggccggcc acacaggcat cgcgcacacg cgctgggcca cgcacggcgt
     7501 gccggcggtg accaacgcgc acccgcacgt cagccgcggc ccggtgccgg gcggcatggg
     7561 cggcgacgag gccgcggcgc gtcccgcccg cgtgacgctg gtgcacaacg gcatcattga
     7621 aaaccacgaa acgctgcgcg ccgagctgct ggcgcgcggc tacttctttg ccagccagac
     7681 cgacaccgaa gtcatcgtgc acctgatcga cagcctgtac gacggcgatc tgctcgccgc
     7741 ggtgcaggcg gcggtgcggc gcctgcgcgg cagctattcg ctggccgtgc tgcaccgcga
     7801 cgagccgcag cgcatcgtcg gcgcgcgcga aggctcgccg ctggtgctgg ggctgggggc
     7861 cgacggcaag gagaacttcc tcgccagcga tgccatggcg ctggccggcg tgaccgacca
     7921 gatcgtctat ctggaagagg gcgacgtggt cgacgtgcag ttggccaagc actggatcgt
     7981 aggccgcgcc ggccaacgcg tgcagcgccc cgtcaagacc gtgcaggcca tgagcggcgc
     8041 ggccgagctg gg
//
LOCUS       DAOMVO010000091         7989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2354_length_7989_cov_13.715906, whole genome shotgun sequence.
ACCESSION   DAOMVO010000091 DAOMVO010000000
VERSION     DAOMVO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_7989_cov_13.715906"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..467
                     /gene="rplO"
                     /locus_tag="PKV30_06095"
     CDS             36..467
                     /gene="rplO"
                     /locus_tag="PKV30_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRL29823.1"
                     /translation="MELNTIKPADGAKRVRRRVGRGIGSGLGKTAGRGHKGQKSRAGG
                     YHKVGFEGGQMPLQRRLPKRGFKSASLKYNAEVTLSDLERLGAAEVDLLVLKQAGLVR
                     AIAKVVKVIKSGELTKAVKLTGVGATAGAKAAIEAAGGSLA"
     gene            494..1813
                     /gene="secY"
                     /locus_tag="PKV30_06100"
     CDS             494..1813
                     /gene="secY"
                     /locus_tag="PKV30_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRL29824.1"
                     /translation="MATSGPQIAKTGKYGDLRRRLVFLLLALVVYRIGAHIPVPGINP
                     DQLRQLFQGQQGGILNLFNMFSGGALSRFTVFALGIMPYISASIIMQLMTYVVPTFEQ
                     MKKEGEAGRRKITQLTRYGTLALALFQSFGIAVALETSQGLVVSPGWGFRMTAVVSLT
                     AGTMFLMWLGEQITERGLGNGISILIFAGIAAGLPNAVGGLMELVRTGAMSVIIALFI
                     VALVCLVTYFVVFIERGQRKILVNYARRQVGNKVYGGQSSHLPLKLNMAGVIPPIFAS
                     SIILLPATVVGWFSAGDSMRWLKDLAGTLTPGQPIYVMLYAAAIIFFCFFYTGLVFNS
                     RETADNLKKSGAFIPGIRPGDQTARYIDKILMRLTLAGAIYITAVCLLPEFLILRYNV
                     PFYFGGTSLLILVVVTMDFMAQVQNYMMSQQYESLLKKANFKTTLNG"
     gene            1880..1993
                     /gene="rpmJ"
                     /locus_tag="PKV30_06105"
     CDS             1880..1993
                     /gene="rpmJ"
                     /locus_tag="PKV30_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRL29825.1"
                     /translation="MRVSASVKKICRNCKIIRRKGVVRVICTDPRHKQRQG"
     gene            2030..2395
                     /gene="rpsM"
                     /locus_tag="PKV30_06110"
     CDS             2030..2395
                     /gene="rpsM"
                     /locus_tag="PKV30_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRL29826.1"
                     /translation="MARIAGINIPPHKHAEIGLTAIFGIGRSRARKICEACGIPYDKK
                     IKDLTDGDQEKIRDAIAQFIIEGDLRRETTMNIKRLMDIGCYRGFRHRRGLPMRGQRT
                     RTNARTRKGPRKAAQALKK"
     gene            2413..2817
                     /gene="rpsK"
                     /locus_tag="PKV30_06115"
     CDS             2413..2817
                     /gene="rpsK"
                     /locus_tag="PKV30_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRL29827.1"
                     /translation="MARAPASNAAQRVRKKVRKNISDGIAHVHASFNNTIITITDRQG
                     NALSWASSGGQGFKGSRKSTPFAAQVASEVAGRAAMEQGIKNLDVEIKGPGPGRESSV
                     RALAALGIRITSISDVTPVPHNGCRPQKRRRI"
     gene            2953..3576
                     /gene="rpsD"
                     /locus_tag="PKV30_06120"
     CDS             2953..3576
                     /gene="rpsD"
                     /locus_tag="PKV30_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRL29828.1"
                     /translation="MARYLGPKAKLSRREGTDLFLKSARRSISDKAKFDTKPGQHGRT
                     SGQRTSDYGLQLREKQKVKRMYGVLEKQFRRYFAEAERRRGNTGANLLFLLESRLDNV
                     VYRMGFGSTRAEARQLVSHKAVTVNGQPVNIPSYLVKTGDVIAVREKSKKQTRVLEAM
                     QLAGQVGFPSWVEVSTDKVEGTFKKVPDRDEFGADINESLIVELYSR"
     gene            3689..4681
                     /gene="rpoA"
                     /locus_tag="PKV30_06125"
     CDS             3689..4681
                     /gene="rpoA"
                     /locus_tag="PKV30_06125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRL29829.1"
                     /translation="MQTQLLKPKAINVEPKGPNKAEVTLEPFERGYGHTLGNALRRVL
                     LSSMIGYAPTEVTIAGVLHEYSSIDGVQEDVVNILLNLKGVVFKLHNRDEVTLSLRKD
                     GEGVVTAADIQTPHDVEIINPEHVIATLSQGGKLDMQIKVEKGRGYVPGTLRRYADEP
                     TKSIGRIVLDASFSPVKRVSYAVENARVEQRTDLDKLVMEIETNGAINAEDAVRASAK
                     ILVEQLAVFAQLEGSELAAFDQPAQRSGAATFDPILLRPVDELELTVRSANCLKAENI
                     YYIGDLIQRTENELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPSGLDKR"
     gene            4894..5280
                     /gene="rplQ"
                     /locus_tag="PKV30_06130"
     CDS             4894..5280
                     /gene="rplQ"
                     /locus_tag="PKV30_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRL29830.1"
                     /translation="MRHGNGLRKLNRTSAHRQAMLRNMMNSLIEHEAIKTTVPKAKEL
                     RRVVEPMITLAKNDSVANRRLAFARLRDDASVAKLFTELGPRYNARPGGYTRILKMGF
                     RVGDNAPMAFVELVDRPEADGEQAAE"
     gene            5303..5379
                     /locus_tag="PKV30_06135"
     tRNA            5303..5379
                     /locus_tag="PKV30_06135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5337..5339,aa:Met,seq:cat)
     gene            complement(5568..6062)
                     /locus_tag="PKV30_06140"
     CDS             complement(5568..6062)
                     /locus_tag="PKV30_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29831.1"
                     /translation="MIKPRLTSLLIAASALSACAAGQNAIETRAPAATSTATTGADCP
                     DVTRLHAAQLHGSWEVELVQAGQRGQLTLRQHPEFSASLRGEFRYGGQRSIASGDVEG
                     GEFNLDESRDGKTLFAFWSGQLVPAACGAEIRGKWQPLAREGQPVLAESDFILRRARG
                     GTGW"
     gene            complement(6059..>7989)
                     /locus_tag="PKV30_06145"
     CDS             complement(6059..>7989)
                     /locus_tag="PKV30_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL29832.1"
                     /translation="VEGQRIAAITFTNKAAAEMRERARELVGKPAGEVLICTFHALGV
                     RLLREDGAALGLKPQFSILDSDDVLGLIKDCGATTDAATARQWQWAISRWKNAGLDAA
                     AALAQAAGEGERQTAQLMARYEERLAAYQAVDFDDLIGLPLRMLQKHEQIAQKWQARL
                     AHVLVDEYQDTNATQYELMKLLVGARGSFTAVGDDDQSIYGWRGATLDNLKRLPHDYP
                     ALKVIKLEQNYRSTGAILRAANNVIGPNPKLFPKTLYSELGEGEPVRVVDCDHEAHEA
                     DRAVARIQSLRQTSPHKEWRDFAILYRANHQARVFEQALRRVQIPYKVSGGQSFFDRA
                     EIKDLCAWLRLWINEDDDPAFLRAVTTPKRGIGHQTLATLGEFASKSRLSLFGALFSH
                     SLSAALPARAVGSLHEFGRELNDQRHRAQHTVGHEAARAFLTDWLKAIGYEKHLYDSS
                     DSEGQAAARWTNVLEFCDWMAGRCGGQIDDAAGVTVTAERKTLLEVAQTISLISTLNE
                     REQDPNVVTLSTLHAAKGLEWPHVVLAGVIEGLLPFRPDEDAGAAAQAARIEEERRLM
                     YVGITRAQRTLAVSWTRRRKKGREIVAAQPSRFIAEMALDKTTVRDDPREKLKALRAE
                     FAQRASEQAARSPQGTTP"
BASE COUNT         1428 a         2583 c         2574 g         1404 t
ORIGIN      
        1 gactacctgg tcaagatcgt ctgaggaatc ggatcatgga actcaacacc atcaaacctg
       61 ccgacggcgc caagcgcgtg cgccgccgcg tggggcgtgg catcggctcc ggcctgggca
      121 agaccgctgg tcgcggccac aagggccaga agtcgcgtgc cggcggctat cacaaggttg
      181 gcttcgaagg cggccagatg ccgctgcagc gtcgcctgcc caagcgcggc ttcaagtcgg
      241 cctcgctcaa gtacaacgcc gaagtaaccc tgtcggatct cgagcgcctg ggtgctgcgg
      301 aggtggacct gctggtactc aagcaggccg gtctcgtgcg cgcgatcgcc aaggtcgtca
      361 aggtcatcaa gtcgggcgaa ctcaccaagg ccgtcaagct gacgggcgtg ggggcgaccg
      421 cgggtgccaa ggctgccatc gaggcggctg gcggctcgct ggcctgagtg tttcaagtca
      481 gaggtattga gtagtggcca ccagcggacc gcaaatcgcg aagaccggca agtacggcga
      541 cctgcgtcgc cgcctggtct tcctgctgct cgcgctggtc gtgtaccgca tcggcgcgca
      601 cattcctgtg cccggcatca acccggacca gctgcgtcag ctgttccagg gccagcaggg
      661 cggcatcctg aacctgttca acatgttctc gggtggtgca ctgtcgcgtt tcacggtgtt
      721 cgccctgggc atcatgccct acatctccgc atcgatcatc atgcagttga tgacctatgt
      781 ggtgccgacc ttcgagcaga tgaagaagga aggcgaggca gggcgtcgca agatcaccca
      841 gctgacgcgc tacggcacgc tggcgctggc gctgttccag tcgttcggca tcgccgttgc
      901 gctggaaacg tcgcagggcc tggtggtcag cccgggctgg ggtttccgca tgaccgcggt
      961 ggtcagcctg acggccggca ccatgttcct gatgtggctg ggcgagcaga tcaccgagcg
     1021 gggcttgggc aacggtatct cgatcctgat cttcgccggc atcgccgcag gcctgccgaa
     1081 cgcggtcggt ggcttgatgg aattggtgcg caccggtgcg atgagcgtca tcatcgcgct
     1141 gttcatcgtg gccttggtgt gcctggtgac ctacttcgtg gtgttcatcg agcgcggcca
     1201 gcgcaagatt ctggtcaact atgcgcggcg ccaggtgggc aacaaggtgt atggtggcca
     1261 gtcgtcgcac ctgccgctga agctgaacat ggccggtgtg atcccgccga tctttgcgtc
     1321 gtcgatcatc ctgctcccgg ccaccgtcgt gggttggttc agcgccggtg attcgatgcg
     1381 ctggttgaag gaccttgccg gtaccttgac accgggccag ccgatctatg tcatgctgta
     1441 cgcggcggcc atcatcttct tctgcttctt ctacacgggc ctggtgttca acagccgtga
     1501 gacggcagac aacctgaaga agagcggtgc gttcattccc ggcattcgtc caggtgatca
     1561 gacggcgcgc tacatcgaca agatcctgat gcgcctgacc ttggctggtg cgatctacat
     1621 cacggcggtg tgcctgctgc cggagttcct gatcctgcgc tacaacgtgc cgttctactt
     1681 cggcggcacc tcgttgctga ttctggtggt ggtgaccatg gatttcatgg cccaggtgca
     1741 gaactacatg atgagccagc aatacgaatc gttgctcaag aaggccaact tcaagacaac
     1801 gctcaacggc tgacggaaat tggtgttcag tggtgcctgc gtggcgccgg ctgaacaaag
     1861 aaagttttag gagagtgaaa tgagagtttc agcttcggtc aagaagattt gccgcaactg
     1921 caagatcatc cgccgcaagg gcgtcgtgcg cgtgatctgc accgacccgc gccacaagca
     1981 gcgccagggt tgatgggtta gcgcccgcta gagttcaaga ggatagacca tggcacgtat
     2041 tgctggtatc aacattccgc cgcacaagca cgccgagatc ggcctgacgg cgatcttcgg
     2101 catcggtcgt tcgcgcgcgc gcaagatctg cgaagcctgc ggcattccct acgacaagaa
     2161 gatcaaggac ttgacggacg gcgatcagga gaaaatccgt gacgccatcg cccagttcat
     2221 catcgaaggc gacctgcgcc gcgaaaccac gatgaacatc aagcgcctga tggacatcgg
     2281 ctgctaccgc ggtttccgtc atcgccgcgg cctgcccatg cgcggccaac gcacgcgcac
     2341 caacgcacgc acccgcaagg gcccgcgcaa ggccgcgcag gcgctgaaga aataatcaag
     2401 gaacgctaag acatggccag agcacccgcc agcaacgccg cgcagcgcgt acgcaagaaa
     2461 gtccgcaaga acatttcgga cggcattgcc cacgtgcacg catcgttcaa caacaccatc
     2521 atcaccatca ccgaccgcca gggcaacgcc ttgtcgtggg catcgtcggg cggccagggc
     2581 ttcaagggtt cgcgcaagtc gacgcctttc gcggcccagg tggcctccga agtggccggc
     2641 cgtgcggcga tggaacaagg catcaagaac ctggatgtcg agatcaaggg ccccggcccc
     2701 ggtcgcgagt cttcggtgcg tgcactggcc gcactgggca tccgcatcac gtccatctcc
     2761 gacgtgacgc cggtgccgca caacggttgc cgtccgcaga agcgccgtag gatctaaaca
     2821 acgaagggtc aacacggcat tgctgcgcat gccgtgtcct tttttgtttt tcgttaagcc
     2881 caccgccgtc cgcttgcatg cagacggctc ccccggcaag cggccggggc agacaacttc
     2941 aaggaaaccc acgtggcacg ttacctcggc cccaaagcca aactttcccg ccgtgaaggc
     3001 accgacctgt tcctgaagag cgcacgccgc tcgatcagcg acaaggccaa gttcgacacc
     3061 aagcccggcc agcacggccg cacttcgggc cagcgcacgt ccgactacgg cctgcagctg
     3121 cgcgagaagc agaaggtcaa gcgcatgtac ggcgtgctgg agaagcagtt ccgccgctac
     3181 ttcgcggaag ccgagcgccg ccgtggcaac accggtgcca acctgctgtt cctgctcgag
     3241 tcgcgcctgg acaacgtggt gtaccgcatg ggcttcggct ccacccgcgc cgaagcgcgc
     3301 cagctggtgt cgcacaaggc cgtcacggtg aatggccagc cggtgaacat cccgtcgtat
     3361 ctggtcaaga ccggcgacgt gatcgccgtg cgcgagaagt cgaagaagca gacccgcgtg
     3421 ctggaggcca tgcaactggc cggccaggtg ggctttccgt cctgggtcga ggtcagcacc
     3481 gacaaggtcg agggcacctt caagaaggtg ccggatcgcg atgaattcgg tgccgacatc
     3541 aacgaatcgc tgatcgtcga actctattcg cgctgatcct tgagtttcag cgcgccatct
     3601 catccgaccc gccgccctgg cgggtttggc gcttcaccag ccttaccggt gtaacgagcc
     3661 gggggtattg agaggaagtc taggaaatat gcaaacccaa ctgctgaaac ccaaagcgat
     3721 caacgtcgag cccaagggcc ccaacaaggc cgaggtcacg ctcgagccgt tcgagcgcgg
     3781 ctatggccac accctgggca acgcgctgcg ccgcgtgttg ctgtcgtcca tgatcggcta
     3841 cgcgccgacc gaggtgacga ttgccggcgt gctgcacgag tactcgtcca tcgacggcgt
     3901 gcaggaagac gtggtcaaca tcctgctgaa cctgaagggc gtggtcttca agctgcacaa
     3961 ccgcgatgaa gtcacgctga gtctgcgcaa ggatggcgaa ggcgtggtca ccgcggccga
     4021 catccagacc ccgcacgacg tcgagatcat caaccccgag cacgtcattg ccacgctgtc
     4081 gcaaggcggc aagctcgaca tgcagatcaa ggtcgagaag ggccgcggct acgtgcccgg
     4141 cacgctgcgc cgctacgccg acgagccgac caagtcgatt ggccgcatcg tgctggacgc
     4201 ttccttctcg cccgtcaagc gcgtgagcta cgcggtcgaa aacgcccgcg tcgagcagcg
     4261 caccgatctc gacaagctgg tgatggagat cgagaccaac ggcgccatca acgccgaaga
     4321 cgccgtgcgt gcgtcggcca agatcctggt cgagcagctg gcggtgttcg cgcagctgga
     4381 aggcagcgaa ctcgccgcct tcgaccagcc ggcacagcgc agcggcgccg ccacgttcga
     4441 cccgatcctg ctgcgtccgg tggacgagct ggagctgacg gtgcgttcgg ccaactgcct
     4501 gaaggccgag aacatctact acatcggcga cctgatccag cgtaccgaga acgagttgct
     4561 caagaccccg aacctgggtc gcaagtcgct caacgagatc aaggaagtgc tggcttcgcg
     4621 tggcctcacc ctcggcatga agctggagaa ctggccgccg tccggtctgg acaagaggta
     4681 aacaagctcc ccctgaggcg ctggcgcgcc ttccccctga gggggacggc gccagtggcc
     4741 gggccaagcc cgttccacgg cgccctcgca tggcctgctc cgcggccatc tgtttttttt
     4801 aagtagtccg ccctttcaaa agggcacacc agtgcaaggg ccgtacctga tccggcggtc
     4861 ctgttaataa agaaacctga aaggaaaagc accatgcgtc acggcaacgg ccttcgcaaa
     4921 ctcaaccgca ccagcgcgca ccgccaggcg atgctgcgca acatgatgaa ctcgctgatc
     4981 gagcacgagg ccatcaagac caccgtgccc aaggccaagg aactgcgccg cgtggtcgag
     5041 cccatgatca cgctggccaa gaacgacagc gtggccaacc gccgcttggc cttcgcccgc
     5101 ctgcgcgacg acgccagtgt ggccaagctg ttcaccgaac tgggcccacg ctacaacgcc
     5161 cgtccgggcg gctacacgcg catcctgaag atgggcttcc gcgtcggcga caacgccccc
     5221 atggcgtttg tcgagctggt ggatcgcccc gaagcggatg gcgaacaagc ggctgaataa
     5281 tctgttataa tttcaggctt accgcgcgat ggagcagtct ggtagctcgt tgggctcata
     5341 acccaaaggt cggaggttca aatccttctc gcgcaaccaa atacctgaca agaagcccgc
     5401 tgatcacagc gggctttttt gtggccggtc gcagcagctc aggctacttc gccggccgtc
     5461 gatgcttcta tattgatagt tgcttgcgca cgtcgggact tgttttcaag aagaaatggc
     5521 ctgaaatcgc tgtggaacgt gcgcgaggcg ctatagatgg agaagcgtta ccagcccgtg
     5581 ccaccgcgtg cgcggcgcag gatgaagtcg ctttcagcca gcacaggctg cccttcgcgc
     5641 gccagcggtt gccacttgcc gcggatctcg gcgccgcagg cagcggggac caactggccg
     5701 ctccagaagg cgaacagcgt cttgccgtcg cgcgattcgt ccaggttgaa ttcgccgcct
     5761 tcgacgtcgc ccgaggcaat cgaccgctgc ccgccgtaac ggaactcgcc gcgcaggctg
     5821 gcgctgaatt cggggtgctg gcgcagcgtg agctggccgc gctggcccgc ctgcaccagc
     5881 tccacctccc agctgccgtg caactgtgcg gcatgcaggc gcgtgacgtc ggggcaatcc
     5941 gcccctgtgg tggcggtcga tgttgcggca ggcgcacggg tttcaatggc gttttggcct
     6001 gcggcacagg cagataaagc gctggcagct atcaataatg aagtcaatcg cggcttgatc
     6061 atggggtagt tccctgcggg ctgcgcgccg cctgctccga cgcgcgctgc gcgaactcgg
     6121 cgcgcagcgc cttgagtttc tcgcgcgggt cgtcgcgcac ggtggtcttg tcgagcgcca
     6181 tttcggcgat gaagcggctc ggctgcgcgg cgacgatctc gcggcccttc ttgcggcgcc
     6241 gcgtccagct cacggccagg gtgcgctgcg cgcgcgtgat gccgacgtac atcagccggc
     6301 gctcttcctc gatgcgcgcg gcctgcgcgg cggcgccggc gtcctcgtcg gggcggaagg
     6361 gcagcagccc ctcgatcacg cccgccagca ccacgtgcgg ccattccagg cccttggccg
     6421 cgtgcagggt ggacagcgtg accacgttcg ggtcctgctc gcgttcgttc agcgtcgaga
     6481 tgagcgagat agtctgcgcc acctccagca gcgtcttgcg ctcggcagtc acggtgacgc
     6541 cggccgcgtc gtcgatctgg ccgccgcagc gcccggccat ccagtcgcag aactccagca
     6601 cgttggtcca gcgcgcggcg gcctggccct cgctgtcgct gctgtcgtac aagtgctttt
     6661 cgtagccgat ggccttcagc cagtcggtga ggaaggcgcg tgccgcctcg tgccccacgg
     6721 tgtgctgcgc gcggtggcgc tggtcgttca actcgcgccc gaactcgtgc aggctgccga
     6781 cggcgcgcgc gggcagggcg gcgcttaagc tgtgcgagaa cagcgcgcca aacagcgaca
     6841 ggcgtgactt ggacgcgaac tcgcccagcg tggccagcgt ctgatggccg atgccgcgct
     6901 tgggcgtggt cacggcgcgc agaaaggcgg ggtcgtcgtc ctcgttgatc cacaggcgca
     6961 gccaggcgca caagtccttg atttcggcgc ggtcgaaaaa gctctggccg cccgacacct
     7021 tgtacggaat ctgcacgcgc cgcagcgcct gctcgaacac gcgcgcctgg tggttggcgc
     7081 ggtagaggat ggcgaagtcg cgccactcct tgtgcggcga ggtctggcgc agcgactgga
     7141 tacgggccac ggcgcggtcg gcctcgtgcg cctcgtggtc gcagtcgacc acgcgcaccg
     7201 gctcgccttc gcccagttcg gaatacagcg tcttcggaaa cagcttgggg ttcggcccga
     7261 tgacgttgtt ggccgcgcgc aggatcgcgc ccgtggagcg gtagttctgc tccagcttga
     7321 tgaccttgag cgccgggtag tcgtgcggca ggcgcttcag gttgtcgagc gtggcgccgc
     7381 gccagccgta gatgctctgg tcgtcgtcgc ccacggcggt gaagctgccg cgcgcgccca
     7441 ccagcagctt catcagctcg tactgcgtgg cgttggtgtc ctggtattcg tccaccagca
     7501 catgggccag ccgcgcctgc cacttctgcg cgatctgctc atgtttttga agcatgcgca
     7561 gcggcaggcc gatcaggtcg tcgaagtcga ccgcctggta ggcggccagg cgctcttcgt
     7621 agcgcgccat cagctgcgcg gtctggcgct cgccttcgcc cgccgcctgc gccagcgcgg
     7681 cggcggcgtc caggccggcg ttcttccagc ggctgatggc ccattgccac tggcgcgcgg
     7741 tggcggcgtc ggtggtggcg ccgcagtcct tgatgaggcc gagcacgtcg tcgctgtcga
     7801 ggatgctgaa ttgcggcttc aagcccagcg ccgccccgtc ttcgcgcagc aggcgcacgc
     7861 ccaaggcgtg aaaggtgcag atcagcacct cgccggccgg cttgcccacg agttcgcgcg
     7921 cgcgctcccg catttcggcg gcggccttgt tggtgaaggt gatggcggca atgcgctggc
     7981 cctcaacgc
//
LOCUS       DAOMVO010000092         7976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2359_length_7976_cov_13.719732, whole genome shotgun sequence.
ACCESSION   DAOMVO010000092 DAOMVO010000000
VERSION     DAOMVO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_7976_cov_13.719732"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1009)
                     /locus_tag="PKV30_06150"
     CDS             complement(200..1009)
                     /locus_tag="PKV30_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29833.1"
                     /translation="MTNLLDSIRPFDKQVPLVGALMAAAVLSACGGSDDPAGTLRLGG
                     TVAGLAAGKSVELQNNGAEALRVSENGRFSFSTPLESGAAYAVTVKTQPDGQTCTVAR
                     GAGTATADVSDVAVSCVAGTAPPPSPLLSGTAALDGEWVNHKVCTPIGGGQFAQQMLK
                     LVRQSDTVVEYRSGTLIHGNATCQGAGSALVSPMGSVTLSRVESNASIAAHWGSWRLV
                     TGSTSYLVLAKPSNDKLCLLGDQNPSILPTLERVAQSAAVQDQQNACYVRR"
     gene            complement(1038..1694)
                     /locus_tag="PKV30_06155"
     CDS             complement(1038..1694)
                     /locus_tag="PKV30_06155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL29834.1"
                     /translation="MPHPSPSARFWDRIAPRYARAAIADQAGYEKTLQRVSGLLAPTH
                     TVLELGCGTGTTALRLAPHARSLLATDASPEMVAIARERLHAQPTPGLTFAVADADLA
                     TFDTAGYDRVLAFNLLHLLADLDQALAAIAHTLRPGGLFISKTPCIAEMNPLITHLAV
                     PLMRAVGKAPPLLRFDEAQLHAAIRRAGLEVIATERHGTRGKDVRAFVVARKPAAVRA
                     "
     gene            1783..2667
                     /locus_tag="PKV30_06160"
     CDS             1783..2667
                     /locus_tag="PKV30_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL29835.1"
                     /translation="MKKFDWNQLHAFLETADTGSLSAAARKLGLTQPTLSRQVAAIEA
                     SLGVTLFERVGKAMALTAAGLDLLEHARAMGAAAESLTLAATGRSQAVEGVVTVSASD
                     IVATCLLPPLVRAMHQKAPGIAIEVIASNALSDLRRREADIAIRNLKPEQPDLIARFI
                     REATAHFYAAEDWVHANGHPRSAADATRCAFVGSDRSGRYLAYLRQHGLPVAESNFSC
                     YVDHTTTHWALVQQGLGIGAMMDEIACATPGMVRVLDDVPPVRFPIWLVTHRELRTSR
                     RIRVVFDWLAEGLSAAPA"
     gene            2682..3323
                     /locus_tag="PKV30_06165"
     CDS             2682..3323
                     /locus_tag="PKV30_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HRL29836.1"
                     /translation="MSARLLSTQPLVCHPATPCAAVRGIEVALHWQHAPEPALLLIYV
                     VRGALNELRLPGRAAAPTRTDGLWQHTCFEAFVGTEGNSAYREFNFAPSADWAAYLFS
                     DERVRDVAAETAASLAPPRIVTSRSRGAVFTLGAEVPAGWLPDAADRSWSLGLSAVIE
                     SADGRLSYWALHHPAPRPDFHQRAGWAAYLPAPEISSKIGRQRTSDAREQLSK"
     gene            3335..4537
                     /locus_tag="PKV30_06170"
     CDS             3335..4537
                     /locus_tag="PKV30_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRL29837.1"
                     /translation="MQFGLERFLEDPALRAPLKGRRVALLAHPASVTRDLTHSLDALA
                     ALPDVKLRAAFGPQHGLRGDKQDNMVESPDFTDPRLGIPVFSLYGEVRRPTIAMMDTF
                     DVLLVDLQDLGCRIYTFITTLRYVLEAAAAHGKAVWVLDRPNPVGRPVEGLTLRPGWE
                     SFVGAGPMPMRHGMTMGELGDWFVDLLKLKVEYRVIEMQGWQPDAAPGFGWPLDRTWI
                     NPSPNAANLSMARAYAGTVMLEGTTLSEGRGTTRPLELFGAPGIDTRRLLADMRALAP
                     DWLRGCVLRECWFEPTFHKHVGQLCAGVQIHVEDPTHYDHAAFQPWRLQALAFKALRL
                     QQPDYPLWRDFPYEYELGKLPIDVINGSPLLREWVDDPAAAPAALDALARADEAAWLQ
                     TRAPHLLY"
     gene            4623..4973
                     /gene="arsC"
                     /locus_tag="PKV30_06175"
     CDS             4623..4973
                     /gene="arsC"
                     /locus_tag="PKV30_06175"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HRL29838.1"
                     /translation="MSPVTLYHNPNCSTSRNALALLRERGIEPVVVEYLKTPPSRDEL
                     KAIADATGEPLRALLRTRQAEYAEQGLDNPALTDDQLLDAMLATPVLINRPIVVTPQG
                     ARLGRPLERVLEVL"
     gene            4978..5643
                     /locus_tag="PKV30_06180"
     CDS             4978..5643
                     /locus_tag="PKV30_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRL29839.1"
                     /translation="MAAPAPGVAAHAAEPLNAPRSRSDLFWSFTWLALQGFGGVLAVV
                     QRELVEKKRWMTREQFIEDWAVAQIMPGPNVVNLSMMIGDRQFGLSGALAALAGMLAL
                     PTVGVLILAMLFASVADQPLAQGALRGMGAVAAGLIIATGIKLIGALRTSAMGATACA
                     LLAGATFGAIAVLRWPLLWVLVSVGGAGCAWAWVCLGRQARPPAPPPAGPALTDADSG
                     QTP"
     gene            5640..6236
                     /locus_tag="PKV30_06185"
     CDS             5640..6236
                     /locus_tag="PKV30_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRL29840.1"
                     /translation="MSLALSAADWLALFTHFLTLSLLAVGGAITAAPDMHRYLVDTRH
                     WLTEDQFNSSIAVAQAAPGPNVLFVGLMGWNVGLNAGGGPSGGWHAYALALLGMAVTM
                     AGMLLPSSVLTYSATRWAHKNRELRAVRAFKTGMAPLVVALLIATGWLLTAAHNQPAR
                     DWPLWGLTAASALIVWRTKLHLLWLIGAGALLGMLGLV"
     gene            complement(6453..7211)
                     /locus_tag="PKV30_06190"
     CDS             complement(6453..7211)
                     /locus_tag="PKV30_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRL29841.1"
                     /translation="MRADDSLTTVRTLLRDARHIAALTGAGISAESGVPTFRDAQTGY
                     WARFKPEDLATEAAFRRDPERVWDWYAERRAGLQDVQPNAGHLALADFERRHPGRLTL
                     ITQNVDGLHQRAGSSGVLALHGNIFEDRWLDDCALARRPQDACNTEQALPGRPPRCGQ
                     CGNMLRPGVVWFGEMLPPAAFAAAERAAAQCEVMLVVGTAGAVYPAAGLAHQARAAGA
                     QVVIVNPHPSELDDVAHHVLVGTAATLLPQLLGS"
     gene            complement(7222..7887)
                     /locus_tag="PKV30_06195"
     CDS             complement(7222..7887)
                     /locus_tag="PKV30_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL29842.1"
                     /translation="MATPARTSATAAATRGRRSYRHGDLRRALLDAGVALAREGGPDA
                     VVLREATRRAGVVPNAAYRHFAGRQALLDGVRIVALDQLALAMEQQMAVAAQVAEPAD
                     RARALLRAVGVAYVGFAESQPGLFRTAFSGTAAPAAPVTEPVDLPANGRDPLALLGSA
                     LDAMVAADVLPAARRPGAEYLAWSTVHGFAVFLLDGPLRSVTADQRDALGERLLAMVE
                     RGL"
BASE COUNT         1230 a         2775 c         2733 g         1238 t
ORIGIN      
        1 aaaggcgccg cccacgctgt atgtaatcga ggacagcaca tgcaacacaa ccggcatggg
       61 cgcatgggaa aaccggggtt ccgacagcgg ctgcatcagt gccaattggc ccaaacgcac
      121 gaggccggcc agcacgggca cggcgctcat cgccagcagc agatgaagcg gacccacgcg
      181 cgcggcagcc ctcatcgaac taccgtctca catagcacgc gttctgctgg tcctgcaccg
      241 ccgcgctttg cgccacacgt tccagcgtcg gcaggatgga ggggttctga tcccccagca
      301 ggcacaactt gtcgttgctc ggtttggcca gcaccagata gcttgtgctt ccggtgacca
      361 aacgccagct gccccagtgc gctgcgatgg aagcattgct ctcgacgcga gaaagcgtca
      421 cgctgcccat cggactgacc agcgcgctgc ccgccccctg gcaggtggcg ttgccatgaa
      481 tcagcgtgcc gctgcggtat tcgaccaccg tgtcgctctg gcgcaccagt ttcagcatct
      541 gctgcgcgaa ctggccacca ccgatgggcg tgcagacctt gtggttgacc cattcgccgt
      601 ccagcgccgc ggtgcccgac agcagcggtg acgggggcgg ggccgtgcca gccacgcaac
      661 tgacggcgac atcgctcacg tcggccgtgg ccgtgcccgc cccgcgcgcc acggtgcagg
      721 tctggccgtc aggctgcgtc ttcaccgtca ccgcataggc cgcgccgctc tccagcgggg
      781 tgctgaagct gaagcgcccg ttctcgctga cgcgcaaggc ctcggcgcca ttgttttgca
      841 gctccacgct ctttcccgcg gccaacccgg caacggtgcc gcccaggcgc agcgtgccgg
      901 ccgggtcgtc actgcccccg caagcagaaa gcacggctgc cgccatcaag gcgccgacca
      961 gcggcacttg cttatcgaac gggcgaatgg aatccagaag gttcgtcatg atttgcgttc
     1021 tatccacagt gaaaaaatca ggcgcgcacc gcagccggct tgcgcgccac cacaaaggcg
     1081 cgcacgtcct tgccgcgcgt gccgtggcgt tcggtggcaa tcacctccag cccggcgcgc
     1141 cggatcgccg cgtgcaactg ggcctcatca aaacgcagca gcggcggcgc cttgcccacc
     1201 gcgcgcatca gcggcacggc caggtgggtg atcagcgggt tcatctcggc gatgcacggc
     1261 gtcttggaga tgaacagccc gcccggccgc agcgtgtgtg caatggcggc cagcgcctgg
     1321 tccaggtcgg ccagcaggtg cagcaggttg aatgccagca cgcggtcgta gccggcggtg
     1381 tcgaacgtcg ccaggtcggc atcggcgacg gcaaacgtca gacccggcgt gggctgggcg
     1441 tgcaggcgct cccgcgcgat ggcgaccatc tcgggtgaag cgtcggtggc cagcaaactt
     1501 ctggcgtgcg gcgccagccg caacgccgtg gtgccggtgc cgcagcccag ctccagcacg
     1561 gtgtgcgtgg gtgccagcag gccgctgaca cgctgcagcg tcttctcgta accggcttgg
     1621 tcggcgatgg cggcgcgggc gtacctgggc gcgatgcggt cccagaagcg cgctgaagga
     1681 gaggggtgcg gcatggcgat tgatttcacg atccgcggat gctatccata tgcgttatcg
     1741 catggaatat cggaaatctg tattggtcat atactttttt gcatgaaaaa attcgactgg
     1801 aatcagttgc atgcgtttct ggaaacggcc gataccggct cgctgtcggc cgccgcgcgc
     1861 aagctggggc tgacgcagcc gacgctcagc cgacaggtgg cggccatcga ggcgagcctg
     1921 ggcgtgacct tgttcgagcg cgtgggcaag gccatggcgc tgaccgccgc cggcctggat
     1981 ctgcttgagc acgcacgcgc catgggcgcg gcggccgagt cgttgacgct ggcggcaacc
     2041 ggccgatccc aggcggtgga aggggtggtc acggtatcgg ccagcgacat cgtggcgacc
     2101 tgcctgctgc cgccgctggt gcgcgccatg caccagaagg cgcccggcat cgccatcgag
     2161 gtgattgcct ccaacgcgct gagcgacttg cggcggcgcg aagccgacat cgcgatccga
     2221 aacctcaagc ccgagcagcc cgacctgatc gcgcgcttca tccgcgaggc cacggcgcat
     2281 ttctacgccg ccgaggactg ggtgcacgcc aacggccacc cacgcagcgc cgcggacgcc
     2341 acccgctgcg cctttgtggg cagcgaccgc tcgggtcgct acctggccta tctgcgccag
     2401 cacggcctgc ccgtggctga atcgaacttc agttgctatg tggaccacac gaccacccac
     2461 tgggcgctgg tgcagcaggg cctgggcatc ggcgcgatga tggacgagat cgcctgcgcc
     2521 acgcccggca tggttcgcgt tctggacgat gtgccgccgg tgcgattccc gatctggctg
     2581 gtcacgcacc gcgagctgcg cacctcgcgc cgcatccgcg tggtgttcga ctggctggcc
     2641 gaggggctgt cggccgcgcc cgcctgacag aatacgcagc catgtccgcg cgcctgctgt
     2701 ccacgcaacc gcttgtctgc cacccggcca cgccgtgcgc ggcggtgcgg gggatcgagg
     2761 tggcactgca ttggcagcac gcgcctgagc cggcactgct gctgatctat gtcgtgcgcg
     2821 gtgcgctgaa cgaattgcgt ttgcccggcc gcgcggctgc acccacccgc accgacggcc
     2881 tgtggcaaca cacctgcttt gaggccttcg tcggcacgga gggcaactcg gcttaccgcg
     2941 agttcaactt cgcgccatcg gccgactggg cggcctacct attcagcgac gagcgggtgc
     3001 gcgatgtggc ggctgaaacg gccgcgtcgc tggcgcctcc gcgcatcgtc accagccgct
     3061 cgcgcggcgc ggtgttcacg ctgggcgccg aggtgccggc cggctggctg cccgacgcgg
     3121 cagaccggtc ctggtcgctg ggcctgtcgg cggtcatcga gtcggccgat gggcgcttgt
     3181 cgtactgggc gcttcaccac ccggcgccgc gccctgattt tcaccagcgc gcgggctggg
     3241 ctgcatacct gccggcgcca gagatttcaa gcaaaattgg ccgccagcgc acgtcagacg
     3301 cgcgcgagca gctatcaaaa taaaagcatt gacgatgcaa ttcggactcg aacgttttct
     3361 tgaagacccg gccctgcgcg cgcccttgaa gggacgccgc gttgcgctgc tggcgcaccc
     3421 cgcctccgtc acgcgcgact tgacgcattc gctcgatgcc ctggccgctt tgcccgatgt
     3481 gaagctccgc gcggccttcg gcccccagca tggcctgcgc ggcgacaagc aggacaacat
     3541 ggtcgagtcg cccgacttca ccgatccgcg tctgggcatt cccgtgttca gtttgtacgg
     3601 cgaggtgcgc cgccccacga tcgccatgat ggacacgttt gacgtgctgc tggtcgatct
     3661 gcaagacctg ggctgccgca tctacacctt catcaccacg ctgcgctatg tgctcgaagc
     3721 cgccgcggca catggcaagg cggtttgggt gctggatcgc cccaacccgg tcggccgccc
     3781 ggtggaagga ctgacgctgc ggccaggctg ggaaagcttc gtcggcgccg gccccatgcc
     3841 gatgcgccac ggcatgacga tgggcgagct gggcgactgg ttcgtcgacc tgttgaagct
     3901 caaggtcgag taccgcgtga tcgagatgca aggctggcag cccgacgccg cgcccggctt
     3961 cggctggcca cttgatcgca cctggatcaa ccccagcccc aacgccgcca acctgtcgat
     4021 ggcgcgcgcc tatgccggca ccgtgatgct ggaaggcacg acgctcagcg aaggccgtgg
     4081 caccacgcgc ccgctggagc tgtttggcgc gcccggcatc gacacgcgcc gcctgctggc
     4141 cgacatgcgg gcgctggcgc ccgactggct gcgcggctgc gtgctgcgcg aatgctggtt
     4201 cgaaccgacc tttcacaagc acgtcggcca gctctgcgcc ggcgtgcaga ttcatgtgga
     4261 agacccaacg cattacgacc acgccgcgtt ccagccgtgg cggctgcagg cgctggcctt
     4321 caaggcgctg cgcctgcagc agccggacta cccgctgtgg cgtgactttc cctacgagta
     4381 cgagctgggc aagctgccca tcgacgtcat caacggcagc ccgctgctgc gcgaatgggt
     4441 ggatgaccct gccgccgcgc ccgccgcgct ggatgcactg gcgcgcgccg acgaggcggc
     4501 gtggctgcaa acgcgcgcgc cgcatctgct gtattgaaag aaaatataag tcaaaacggc
     4561 cgctggcgca cgccaggcaa gcgcaagcag ctatcaattt catagaaaac ggaaaacggt
     4621 cgatgagccc cgtcaccctg taccacaacc cgaattgcag cacctcgcgc aacgcgctgg
     4681 cgctgctgcg cgagcgcggc atcgagcccg tggtcgtcga atacctgaag acgccgccca
     4741 gccgcgacga gctgaaggcc attgccgacg ccaccggcga gccgctgcgc gcgctgctgc
     4801 gcacgcgcca ggccgaatac gccgagcagg ggctggacaa ccccgcgctg accgacgacc
     4861 agttgctcga cgccatgctg gccacgcccg tgctcatcaa ccgccccatc gtcgtcacgc
     4921 cgcagggcgc gcgcctgggc cggccgctgg agcgcgtgct cgaagtgctg tagcgccatg
     4981 gccgcgcccg cccctggcgt tgccgcccac gccgccgaac cgctgaacgc cccgcgttcg
     5041 cgcagcgacc tgttctggtc cttcacctgg ctcgcgctac aaggctttgg tggcgtgctg
     5101 gcggtggtgc agcgcgagct ggtcgagaaa aaacgctgga tgacgcgcga gcagttcatc
     5161 gaggactggg cggtggcgca gatcatgccc ggccccaacg tggtcaacct gtcgatgatg
     5221 atcggcgacc gccaattcgg cctttcgggc gcgctggcgg cgctggccgg catgctggcc
     5281 ttgccgacgg tgggcgtgtt gatcctggcc atgctgtttg ccagcgtggc cgaccagccg
     5341 ctggcgcagg gcgcgctgcg cggcatgggc gcggtggcgg cggggctgat catcgccacc
     5401 ggcatcaagc tgatcggcgc gctgcgcacc agcgccatgg gcgccacggc ctgcgcgctg
     5461 ctggcggggg ccacctttgg cgccatcgcc gtgctgcgct ggccgctgct gtgggtgctg
     5521 gtcagcgtgg gcggcgcggg ctgcgcctgg gcctgggttt gcctggggcg gcaggcgcgg
     5581 ccgccagcgc cgccaccggc cggcccggcg ctgaccgatg ccgattcggg gcagacgcca
     5641 tgagcctcgc gctgtctgcc gccgactggc tggcgctgtt cacgcacttt ctcacgctgt
     5701 cgctgctggc cgtgggcggc gccatcaccg cggcgcccga catgcaccgc tacctggtcg
     5761 acacccgcca ctggctgacc gaggaccagt tcaattcgtc catcgccgtg gcgcaggccg
     5821 cgcccggccc caacgtgctg tttgtcggcc tgatgggctg gaacgtcggc ctgaacgcgg
     5881 gcggcgggcc atctggcggc tggcacgcct acgcgctggc gctgctgggc atggcggtga
     5941 ccatggccgg catgctgctg ccgtcgagcg tgttgaccta cagcgccacg cgctgggcgc
     6001 acaagaaccg cgagctgcgc gcggtgcgtg ccttcaagac cggcatggcg ccgctggtgg
     6061 tggcgctgct gatcgccacc ggctggctgc tcacggccgc gcacaaccag ccggcgcgcg
     6121 actggccgct gtgggggctg acggccgcca gcgcgctgat cgtgtggcgc accaagctgc
     6181 acctgctgtg gctgatcggc gctggggcgc tgctggggat gctggggctg gtgtaagtcc
     6241 attcaggttc actgaaggca ctcaccgcag aggcgcagag aacgcagagc ctcgcagagg
     6301 aaaaccaccg gaattctcgg ggaagctctg cgttccctgc gcctctgcgg tgaatcgttg
     6361 tgcgacctga gacaaggcgc cgatcatgcc aaatcggccg ctggcgcttg atggacaagc
     6421 gcgggcagct attcaatcca tagcgaatga cttcaagaac cgagcagttg cggcagcagc
     6481 gtcgccgccg tgcccaccag cacgtggtgc gccacgtcgt ccagctcgct cgggtgcggg
     6541 ttgacgatga ccacctgcgc acccgccgcg cgcgcctggt gcgccaaccc cgccgccggg
     6601 tacacggcgc ctgcggtgcc gacgaccagc atcacctcgc actgcgcggc cgcacgttcg
     6661 gcggcggcaa aggcagcggg cggcagcatc tcgccaaacc acaccacgcc cgggcgcagc
     6721 atattgccgc actggccgca gcgcggcggc cggccaggca gcgcttgctc ggtgttgcag
     6781 gcatcctgcg ggcgacgcgc cagcgcgcag tcatcgagcc agcggtcttc aaaaatgttt
     6841 ccgtgcagtg ccagcacgcc gctgctgccg gcacgctggt gcaggccatc gacgttctgc
     6901 gtgatcagcg tcaggcgccc cgggtggcgg cgctcgaagt cggccagcgc caggtgaccg
     6961 gcgttcggct gcacgtcttg cagcccggcg cggcgctcgg cataccagtc ccacacgcgc
     7021 tcggggtcgc ggcgaaaggc ggcttcggtc gccagatctt cgggcttgaa gcgcgcccaa
     7081 tagccggttt gcgcatcgcg gaaggtgggc acgcccgatt cggcgctgat gccggcgccg
     7141 gtcagcgcgg cgatgtgccg ggcatcgcgc agcagcgttc gcacggtggt cagtgaatcg
     7201 tcggcgcgca tcgtgattcg gtcaaagtcc gcgttcgacc atggcgagca ggcgttcgcc
     7261 cagcgcgtcg cgctgatccg ccgtcacgct gcgcagcggg ccgtcgagca ggaagaccgc
     7321 gaagccgtgc accgtgctcc aggccaggta ttcggcgcct ggccggcgcg cggcgggcag
     7381 cacatcggcg gcgaccatgg catcgagcgc gctgcccagc agcgccagcg ggtcgcgccc
     7441 gttggccggc agatcgaccg gctcggtcac cggcgccgca ggcgcggcgg tgccggagaa
     7501 cgcggtgcga aacaggcccg gctggctttc ggcaaaaccc acgtaggcca cgccgacggc
     7561 gcgcagcagc gcgcgcgcgc ggtcggccgg ctcggccact tgcgcggcga cggccatttg
     7621 ctgctccatc gccagcgcca gctggtccag cgcgacgatg cgcaccccgt ccagcaaggc
     7681 ctgccggccg gcaaagtgcc ggtaggcggc gttgggcacc acgccagcgc ggcgcgtggc
     7741 ctcgcgcagc accaccgcgt cgggcccgcc ctcgcgcgcc agcgccacgc cagcatccag
     7801 caaggcacgc cgcaaatcgc cgtggcgata gctgcggcgc ccgcgcgtgg cggcggccgt
     7861 ggcagaggtg cgggcgggtg tagccatgtg cggggcaggg ttttatgttg gcgccatcca
     7921 tcctagggca gaatgatgtg gacgttgtac acatcacaac gccctaccgt gaaaca
//
LOCUS       DAOMVO010000093         7967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2362_length_7967_cov_11.723079, whole genome shotgun sequence.
ACCESSION   DAOMVO010000093 DAOMVO010000000
VERSION     DAOMVO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_7967_cov_11.723079"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKV30_06200"
     CDS             <1..585
                     /locus_tag="PKV30_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRL29843.1"
                     /translation="AKSMFDAAQVLGAAVRHISQRDGAALRQSGLEFNVSLILGGQIA
                     GEGMRLFLVYSAGNFIEATAETPYFQIGESKYGKPVLDRVITPETPLDEAAKCALVSM
                     DSTLKSNLSVGLPLDLVVYEVDRLQSDRVVCIDQDNPYFTMLHKSWGQRLREVFDSIE
                     DPQWNGGATEVPLRVPSARYQVLKKITQADERVI"
     gene            684..>1418
                     /locus_tag="PKV30_06205"
     CDS             684..>1418
                     /locus_tag="PKV30_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL29844.1"
                     /translation="MSLIVFSHGNSFPASTYRVMLDSLRARGFTVHAIEKYGHDPRYP
                     VTDNWPHLVQQLADFAREQRQAGGEAPYLVGHSLGGILSLMCAARHPELARGVVMLDS
                     PVLGGWRAGAIGVAKHTPLMKRLSPSVVSRKRRHQWDDREAVFQHFRGKKLFAAWDEQ
                     VLHDYVNHGTLDAEGTWQLAFDREVESRIYDTLPHNIGTLLRAQPLKCPLAFIGGLQS
                     AEMKQVGGLENTARIAKGRVMMIDGSH"
     assembly_gap    1419..1518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1519..2075)
                     /locus_tag="PKV30_06210"
     CDS             complement(<1519..2075)
                     /locus_tag="PKV30_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRL29845.1"
                     /translation="MNNQCSLFADEAIELVRDAEGGICYHPACVPEDVAAGWLAALLP
                     QVPWRSERRPMYDRIVDVPRLMASVALNDPDRPPCIDAALAAVQAVAPAPYTRVGLNL
                     YRGAQDSVAMHNDRTHDLAPGQPIAILSLGAPRDMLIRAKQGGASQRVRLDPGSVLVM
                     SHASQHTHEHGIPKTADGAGPRISLA"
     gene            complement(2187..2768)
                     /locus_tag="PKV30_06215"
     CDS             complement(2187..2768)
                     /locus_tag="PKV30_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL29846.1"
                     /translation="MSASSPAAGPTLWLLRHAPVLAAPGLCYGRTDLPADPAATRRAA
                     ESLALQLPRGTVVRTSPLQRCELLALDLQALRPDLAFKTDARLAEMDFGAWEGQPWGH
                     IPRAAFDAWLADFADAPPGEGGETVRALMARVGAAWDDWRASGADALWVTHAGVMRAA
                     LLLARGVRLPASAADWPAADLPFGEVLALQPTP"
     gene            complement(2765..3568)
                     /locus_tag="PKV30_06220"
     CDS             complement(2765..3568)
                     /locus_tag="PKV30_06220"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRL29847.1"
                     /translation="MQALRHFLLALQFFTRIPITGRLADWVGYSPAMLRASAGHFPGV
                     GWVVGAVAAAVLALALYLLPPGPLGALVAAGLATAATVWLTGAFHEDGLADTADGLGG
                     SASKERALAIMKDSRIGSYGAVTLVLVVGLKIALTALLAERGAALAAIAVLYAHVLSR
                     AAPLDVMWGLPYIGDADGSKSKPVAESITRESIWTGLAWALPALGLMWWALGLWQIAV
                     AIVPWMIALGQMRRLLLRRLEGFTGDTLGATQQLCELALLLGVALVVPR"
     gene            complement(3797..4834)
                     /gene="cobT"
                     /locus_tag="PKV30_06225"
     CDS             complement(3797..4834)
                     /gene="cobT"
                     /locus_tag="PKV30_06225"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRL29848.1"
                     /translation="MSLALPAIADLHDAALTAALQHKIDRKTVPYGALGRLGALAQRL
                     GLILNTTEPRLQAPQLVVFAADHGVAARGVSAYPSDVTWQMVGNFLAGGAAVSVLARQ
                     HGIAQTVVDCGVRHDFDAAHGLLVKKIAPGTADFSQGPAMSAAQRDEALVNGMEIVKS
                     LPGNALLLGEMGIGNTSPASCLLARLAGIDVGEVTGAGTGLDEAGIARKTAVLREALA
                     ANAGATEPLDALAALGGFEIATMVGSVLQAARERRVIVVDGFIASSAVLVASRIAPHV
                     LQRCVFAHQSDERGHARLLQVLGAEPLIDLGLRVGEGSGAALAWPLLESACRIINEMA
                     SFESAGVSDKG"
     gene            complement(4867..5862)
                     /locus_tag="PKV30_06230"
     CDS             complement(4867..5862)
                     /locus_tag="PKV30_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRL29849.1"
                     /translation="MRLEGRRSSGSGRLRRWAVNRLPWLAGAAVLGASLPLWQAWVPT
                     GIGRWLADLAVHWQWPYALIGVGSAALWLALRARGERGGAAAMLAAALVLAALNLSWT
                     PLPRLPRAEAAAAVPIKLASFNVHLDNRAFDAIANWASAEQPDVLALVEVTPDMQPLL
                     ARLAARYPHTALHARADPFGLAVFSRHPLQGAQLHDDGRLPPRWRATVKAPGAPFTLT
                     VVHPMPPISAADRAERDALFDALAQQPASTRAQVIMGDFNSTPWSAGLRRVGDAGWAR
                     ATTLHPTFALGHSLPLDHILATRAHWRVADAGMGPWLGSDHRPVWALLHAVRGDP"
     gene            5861..6625
                     /locus_tag="PKV30_06235"
     CDS             5861..6625
                     /locus_tag="PKV30_06235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL29850.1"
                     /translation="MSTVHTAAQQGYSRHADAYASGRPDYPGALQDWLRDALGLGPAR
                     TVADVGAGTGKFTPLLLATGADVVAVEPVEAMRARIAPHERLRVQAGKAEATGLSSGS
                     LGAIVCAQAFHWFSSRATLDEFARVLKPGGRLGLVWNVRDEPVDWVAELTRIVAPYEG
                     DAPRFWKQTWREAFPHPALGPAHETVFEHEHVGPPAQVIVDRFMSVSFTAALTADEQA
                     RVRAQIEALIASHPALRGREVLRFPYQTRALWFARH"
     gene            complement(6619..>7967)
                     /locus_tag="PKV30_06240"
     CDS             complement(6619..>7967)
                     /locus_tag="PKV30_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRL29851.1"
                     /translation="YFQALAARAVPDVRMNPLLLKPEADTRSQVVLMGQVSCELSALP
                     WRGRSERVWPQIAAALDALRAENDVIVIEGAGSPAEINLMASDVVNLRVARHAGARCL
                     LVTDIDRGGAFAHLYGTWALMAPEDRARIAGFVLNKFRGDAALLAPAPEQLEQLTGVP
                     TVAVVPMRLGHGLPEEDGVFDDRAVLAPSPAGGGQASGQATPALSLSKGGEGTRPRHI
                     AVVAYPRISNLDEFQPLKNVPGLTLSWARTPAALYGADTLILPGSKATAADLAWLRAQ
                     GLDAAIAEHAARGGRVLGICGGLQMLGEALIDVHGIEAQGNAPGLGLLPLVTSFEPDK
                     TVRRTQTRFGDVVGAWGALSAVPIGGYEIHAGQTAPHPAMVAKGDVPREVIPGIAWQN
                     GGGNVLGVYLHGLFEDAAVLQALFGAGAPSLDAVFDQLAANVERAFAPGVLQGLIQ"
BASE COUNT         1254 a         2828 c         2671 g         1114 t
ORIGIN      
        1 gccaagagca tgttcgacgc cgcccaggtg ctgggcgcgg cggtgcgcca catcagccag
       61 cgcgacggcg ccgcgctgcg ccagtcgggg ctggaattca acgtctcgct catcctgggc
      121 ggccagatcg cgggcgaagg catgcgattg tttctggtct attcggccgg caacttcatc
      181 gaggccacgg ccgaaacgcc ctactttcag attggcgaat cgaaatacgg caagcccgtg
      241 ctcgaccgcg tgatcacgcc cgagacgccg ctggacgaag ccgccaagtg cgcgctggtg
      301 tcgatggatt cgacgctcaa gtccaacctg tcggtcgggc tgccgctgga cctggtggtg
      361 tacgaggttg atcggttgca gtccgaccgc gtggtctgca tcgaccagga caacccctac
      421 ttcaccatgc tgcacaagag ctggggccag cgcctgcgcg aggtgttcga cagcattgaa
      481 gacccgcagt ggaacggcgg cgccaccgag gtgccgctgc gcgtgccctc ggcgcgctac
      541 caggtgctga aaaaaatcac ccaggccgac gagcgggtga tctgacccgt caccctggga
      601 cgcgtcgggc ccacgcccag cgcagccaac acaccggctg ggcggcactg gtgtaataag
      661 cgtgttccac tgtctgcgtc tccatgagcc tgatcgtttt ctcccacggc aacagctttc
      721 ccgccagcac ctaccgcgtg atgctggaca gcctgcgcgc acgcggtttc accgtgcacg
      781 ccatcgaaaa atacggtcac gacccgcgct accccgtcac cgacaactgg ccgcacctgg
      841 tgcagcagct ggccgacttt gcgcgcgagc aaaggcaggc cggcggcgag gcgccctacc
      901 tggtcggcca ttcgctgggc ggcatcctga gcctgatgtg cgccgcgcgc caccccgagc
      961 tggcgcgtgg cgtggtgatg ctcgattcac ccgtgctggg cggctggcgc gccggcgcga
     1021 tcggcgtggc caagcacacg ccgctgatga agcggctgtc gcccagcgtg gtcagccgca
     1081 agcgccgcca ccagtgggac gaccgcgagg cggtgttcca gcactttcgc ggcaagaagc
     1141 tgtttgcagc gtgggacgag caggtgctgc acgattacgt caaccacggc acgttggacg
     1201 ccgaaggtac gtggcaactg gcgttcgacc gcgaggtcga atcgcgcatc tacgacacgc
     1261 tgccgcacaa catcggcacg ctgctgcgcg cgcagccgct gaagtgcccg ctggccttca
     1321 tcggcgggtt gcagtcggcc gagatgaagc aggtgggcgg gctggagaac accgcccgca
     1381 tcgccaaggg gcgggtgatg atgatcgacg gctcgcacnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnngc caggctgatg cgcgggccgg cgccatcggc agttttggga
     1561 atgccgtgct cgtgcgtgtg ctggctggcg tggctcatca ccagcacgct gccggggtcg
     1621 agccgcacgc gctggctggc gccgccctgc ttggcgcgga tcagcatgtc gcgcggcgcg
     1681 ccgagcgaga gtatggcgat cggctgaccg ggcgccaggt cgtgcgtgcg gtcgttgtgc
     1741 atggccacgc tgtcttgcgc gccgcggtac aggttgaggc cgacgcgcgt gtagggcgcg
     1801 ggcgccaccg cctgcaccgc cgccagcgcg gcgtcgatgc agggcgggcg gtcggggtcg
     1861 ttcagcgcca cgctggccat caggcgcggc acgtcgacga tgcggtcgta catcggccgc
     1921 cgttcgctgc gccacggcac ctgcggcagc agcgcggcca gccagccggc cgccacgtct
     1981 tcgggcacgc aggccgggtg gtagcagatg ccgccttcgg catcgcgcac cagttcgatt
     2041 gcttcgtcgg caaacaatga gcactggttg ttcatccagt gatcttaacg ctgcgtcggc
     2101 cgcgcttcaa tgacgccgtg ggagcggact tgcccgcgat gcgactgatc tctggccgcg
     2161 ccggcccatc gcgggcaagc ccgctcctac ggcgtcggct gcagcgccag cacttcgcca
     2221 aacggcaggt cggcggccgg ccaatcggcg gcgctcgcgg gcaggcgcac gccgcgcgcg
     2281 agcagcagcg cggcgcgcat cacgccggcg tgggtgaccc acagcgcatc ggcgccgctg
     2341 gcacgccagt cgtcccacgc ggcaccgacg cgggccatca gcgcgcgcac cgtttcgccg
     2401 ccctcgcccg gcggcgcatc ggcgaagtcg gccagccagg cgtcgaaagc ggcgcgcggg
     2461 atgtggcccc agggctggcc ttcccaggcg ccgaagtcca tctcggccag gcgcgcatcg
     2521 gttttgaatg ccaaatcagg ccgcagcgct tgcagatcaa gcgccagcag ctcacatctt
     2581 tgtagcggcg aagttctgac caccgtgccg cgtggcagtt gcaacgccaa gctctcggcg
     2641 gcgcggcgcg tggcggcggg gtcggccggc agatcggtgc ggccgtaaca caggccgggc
     2701 gcggccagca ccggcgcgtg gcgcagcaac cagagcgtgg ggccggcggc cggcgacgag
     2761 gcactcatct cggcacgacc agcgccacgc cgagcagcaa ggccagttcg cacagttgct
     2821 gcgtggcgcc gagtgtgtcg ccggtaaagc cttccaaacg ccgcaacaac aagcgccgca
     2881 tttggcccag cgcgatcatc cacggcacga tggcgacggc gatttgccac agcccgagcg
     2941 cccaccacat caagcccagc gccggcagcg cccaggccaa gccggtccaa atactctccc
     3001 gcgtgatcga ctccgccacg ggcttgctct tgctgccgtc cgcgtcgccg atgtagggca
     3061 agccccacat cacatccagc ggcgccgcgc gcgacagcac gtgcgcgtac agcaccgcta
     3121 tggccgccaa cgccgcgcca cgctcggcca gcagcgccgt cagcgcgatc ttcaaaccca
     3181 ccaccagcac cagcgtgacc gcgccgtagc tgccgatgcg cgagtccttc atgattgcca
     3241 gcgctctttc tttcgaagcg ctgccgccca gcccgtcggc ggtatcggcc aggccgtctt
     3301 catgaaaagc gccggtcagc cacaccgtgg cggccgtcgc cagcccggcc gccaccaacg
     3361 cgcccagcgg ccctgggggc agcaggtaaa gcgccaacgc cagcacggcc gccgccaccg
     3421 cgcccaccac ccagccgacg ccggggaaat gccccgcact ggcgcgcagc atggccgggc
     3481 tgtatccgac ccaatccgcc agccgcccgg tgatggggat gcgggtgaag aactgcagcg
     3541 cgagcaggaa gtggcgcagg gcttgcaagg gcttcatggg gccggatgcg ctgaacgaat
     3601 accggacgcg aaggacgcaa gagatacgcg aaggacgcga aaggaattca tatttttata
     3661 gctgcttgcg ctggctgcac aagcaccagc acggtatttc attgaaaatc cccatggaca
     3721 gtttactttt tcaggctgtt ctttcgcgtc cttcgcgaac ccttcgcgta cggctgtttc
     3781 ggggccgcaa aacgcttcaa cccttgtccg acacgcccgc cgattcaaag ctcgccatct
     3841 cgttgatgat gcggcaggcc gactccagca gcggccaggc cagcgccgcg ccgctgccct
     3901 cgcccacgcg caggccgagg tcgatcagcg gctcagcacc cagcacctgc aacagccgtg
     3961 cgtggccgcg ctcgtccgac tggtgcgcaa acacgcagcg ctgcagcacg tgcggcgcga
     4021 tgcggctggc caccagcacc gcgctgctgg cgatgaagcc atcgaccaca atcacgcgcc
     4081 gttcgcgcgc tgcttgcaac acgctgccga ccatcgtcgc aatctcgaag ccgcccagcg
     4141 cggccagcgc atccagcggc tcggtcgcgc ccgcgttggc ggccaatgcc tcgcgcagca
     4201 ccgccgtctt gcgggcgatg ccggcttcat ccagccccgt gccggcacca gtgacttcgc
     4261 ccacgtcgat gccggccagg cgcgccagca ggcaactggc gggcgaggtg ttgccaatgc
     4321 ccatttcgcc cagcagcagc gcgttgccgg gcaggctttt gacgatctcc atgccgttga
     4381 ccagcgcttc atcgcgctgc gcggcgctca tcgccgggcc ctggctgaag tcggcggtgc
     4441 cgggggcgat cttcttcacc agcaggccgt gcgcggcgtc aaagtcgtgc cgcacgccgc
     4501 aatcgaccac cgtctgcgca atgccatgct ggcgcgccag cacgctcacc gcagcgccgc
     4561 cggccaaaaa gttgcccacc atctgccagg ttacgtcact cggatacgcc gacacgccgc
     4621 gcgcggccac gccatggtcg gcggcaaaca cgaccagttg cggcgcctgc aggcgcggct
     4681 cggtcgtgtt caggatcaaa cccagccgct gcgccagcgc ccccagccgg cccagcgcac
     4741 cgtacggcac ggtcttgcgg tcgatcttgt gttgcagggc ggcggtcagc gcggcatcgt
     4801 gcaggtcggc gatggcaggc agggcaagag acatggcgat ccaatttcca aaggtcaaac
     4861 aaaaagtcaa gggtcaccgc gcaccgcgtg cagcagcgcc cacacaggcc ggtggtcgga
     4921 gcccagccac ggccccatgc ccgcatcggc cacgcgccag tgcgcgcgcg tggccagaat
     4981 gtggtcgagg ggcaggctgt gccccaacgc aaaggtgggg tgcaaggtgg tggcgcgcgc
     5041 ccagcccgca tcgcccacgc ggcgcaggcc ggcgctccac ggcgtgctgt tgaagtcacc
     5101 catgatcacc tgcgcgcggg tagacgcagg ctgctgggcc agtgcgtcga aaagcgcgtc
     5161 gcgctcggcc cggtcggcag cggaaatcgg cggcatgggg tgcacgaccg tgagcgtgaa
     5221 tggcgcgccg ggcgccttca cggtggcgcg ccagcgcggc ggcaggcgcc cgtcgtcatg
     5281 tagttgtgcg ccctgcagcg gatggcgcga gaacacggcc agcccgaacg gatcggcccg
     5341 cgcgtgcagc gccgtgtgtg ggtagcgcgc ggccagccgg gcgagcagcg gctgcatgtc
     5401 gggcgtgact tcgaccagcg ccagcacgtc tggctgctcg gcgctcgccc agttggcgat
     5461 ggcgtcgaac gcgcggttgt ccaggtgcac gttgaaactg gcgagtttga tgggcaccgc
     5521 cgcggcggcc tcggcgcgcg gcaagcgcgg cagcggcgtc cagctgaggt tgagcgccgc
     5581 cagcaccagc gccgccgcca gcatggccgc cgcgccaccg cgttcgccac gcgcgcgcaa
     5641 cgccagccac agcgccgcgc tgccgacgcc gatcagcgcg tacggccatt gccaatgcac
     5701 cgccagatcg gccagccagc gcccgatgcc cgtcggcacc cacgcctgcc acaatggcag
     5761 cgaagcgccc agcaccgccg cccccgccag ccacggcagg cggttgacgg cccagcgccg
     5821 cagcctgccg ctccctgacg accgacgccc ttcaagccgc atgtccaccg ttcacaccgc
     5881 cgcgcagcaa ggctacagcc gacacgcgga cgcctacgcg tcgggccggc ccgactaccc
     5941 cggcgccttg caagactggc tgcgcgatgc gctcggcctg gggccggcgc gcaccgtggc
     6001 cgacgtgggc gcgggcaccg gcaaattcac acccttgctg ctggccaccg gcgccgatgt
     6061 ggtggcggtg gagccggtcg aggccatgcg cgcgcgcatc gcgccgcacg aacgcctgcg
     6121 cgtgcaggcc ggcaaggccg aggcgacggg cttgtccagc ggctcgctcg gcgccatcgt
     6181 ctgcgcgcag gccttccact ggttcagcag ccgcgccacg ctggacgagt tcgcacgcgt
     6241 gctcaagccc ggtggccggc tgggcctggt gtggaacgtg cgcgatgagc cggtcgattg
     6301 ggtggccgag ctgacgcgca tcgtcgcgcc ctacgaaggc gatgcgccgc gcttttggaa
     6361 gcagacctgg cgcgaggcgt ttccgcaccc cgccctcggg ccggcgcacg agacggtgtt
     6421 cgagcacgaa cacgtcggcc cgcccgcgca ggtgatcgtg gaccgcttca tgtcggtcag
     6481 cttcaccgcc gcgctgacgg ccgatgaaca ggccagggtg cgcgcgcaga tcgaggcgct
     6541 gatcgcgtcg caccctgcgc tgcgcgggcg tgaggtgctg cgttttccgt atcagacgcg
     6601 ggccttgtgg ttcgcgcgtc actgaatcaa cccttgcagc acacccggcg cgaaggcgcg
     6661 ctcgacattg gccgccagct ggtcaaacac cgcatctagc gatggcgccc cagcgccaaa
     6721 cagcgcctgc agcaccgccg catcctcgaa caggccgtgc aagtacacac ccagcacgtt
     6781 gccgccgccg ttctgccacg cgatgccggg gatgacttcg cgcggcacat cgcccttggc
     6841 gaccatggcg gggtgcggcg cggtctgacc ggcgtggatc tcgtagccgc cgatcggcac
     6901 agccgagagc gcgccccaag cgcccaccac gtcgccaaag cgcgtttgcg tgcggcgcac
     6961 ggtcttgtcg ggctcgaagc tggtgaccag cggcagcagg ccgaggcccg gcgcgttgcc
     7021 ttgcgcttca ataccgtgca cgtcgatcaa cgcctcgccc agcatttgca ggccgccgca
     7081 gatgcccagc acgcggccgc cgcgcgcggc atgttcggca atcgccgcat cgagcccctg
     7141 cgcgcgcagc caggcgaggt cggcggccgt cgccttgctg ccaggcagga tgagggtgtc
     7201 ggcgccgtac agcgcggcgg gcgtgcgcgc ccagctcaaa gtcaagccgg gcacgttctt
     7261 cagcggctgg aattcgtcga ggttgctgat gcgcgggtag gcgacgacgg cgatgtggcg
     7321 cggccgcgtg ccctcacccc ccttcgacaa gctcagggca ggcgtggcct gccccgacgc
     7381 ctgccctccc ccagcggggg agggagccaa gaccgcgcgg tcgtcgaaca caccatcttc
     7441 ttccggcaac ccatgcccca agcgcatggg caccaccgcc acggtgggca cgccggtgag
     7501 ttgctccagt tgctcgggcg ccggcgccag cagcgccgca tcgcccctga acttgttgag
     7561 cacaaagccg gcaatgcgcg cgcgatcttc cggcgccatc agcgcccacg tgccgtacag
     7621 gtgcgcaaac gcgccgccgc ggtcgatgtc ggtcaccagc aggcagcgcg caccggcgtg
     7681 gcgcgccacg cgcaggttga cgacgtcgct ggccatcagg ttgatctcgg ccggcgagcc
     7741 ggcgccttcg atcacgatca cgtcgttttc tgcgcgcaag gcatctagcg cggcggcgat
     7801 ctgcggccac acgcgctcgc tgcggccgcg ccacggcaag gccgagagtt cacagctgac
     7861 ctgccccatc agcaccacct gactgcgcgt gtccgcctcg ggcttgagca agagcgggtt
     7921 catgcgcacg tcgggcacgg cgcgcgcggc cagggcctgg aagtact
//
LOCUS       DAOMVO010000094         7955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2368_length_7955_cov_16.606456, whole genome shotgun sequence.
ACCESSION   DAOMVO010000094 DAOMVO010000000
VERSION     DAOMVO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_7955_cov_16.606456"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="PKV30_06245"
     CDS             <1..1595
                     /locus_tag="PKV30_06245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228527.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRL29852.1"
                     /translation="RTALTCATRFGICAKCYGRDLGRGGLVNQGEAVGVIAAQSIGEP
                     GTQLTMRTFHIGGAASRAAVASSVEAKSNGVIGFNPTMRYVTNSKGELVVISRSGEIV
                     LHDEHGRERERHKVPYGATLTVKADQQIKAGTILANWDPLTRPIITEFAGQVRFENVE
                     EGLTVAKQVDEVTGLSTLVVIDPKRRGSAKIVRPQVKLVDAGGNEVKIPGTDHSVTIG
                     FQVGALIQVRDGQDVGPGEVLARIPVEGQKTRDITGGLPRVAELFEARSPKDKGTLAE
                     LTGTVSFGKETKGKIRLQITDPDGKVWEELIPKEKNILVHEGQVVNKGESIVDGPADP
                     QDILRLLGMEELARYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRVVVENPGDSGYI
                     AGEQVERSEMLDTNDALRAEGKLPATYTNLLLGITKASLSTDSFISAASFQETTRVLT
                     EAAIMGKRDELRGLKENVIVGRLIPAGTGLAYHQARKVKDQMDEAERRAIADAEAAEL
                     AGEVEVVEGGESASVDEGASAD"
     gene            1669..2253
                     /locus_tag="PKV30_06250"
     CDS             1669..2253
                     /locus_tag="PKV30_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRL29853.1"
                     /translation="MFDSSWWLGLAAALLFFWGVGGYNRLVRLRAAVTSAFAALDEQL
                     VRQLVWVQGCLPDAMRGGVPTLPGELQDDVTAAWARLQAASEQFAVALAHARAQPIDV
                     QRMASLVMAHEALRTAWGATLAEAVPADAVPSSERLQARWLRLLHQSLPLRTAFNDAA
                     QSYNQAIAQFPASLLARLFGFKPAGTVTRLADGR"
     gene            2258..>3452
                     /gene="rsmB"
                     /locus_tag="PKV30_06255"
     CDS             2258..>3452
                     /gene="rsmB"
                     /locus_tag="PKV30_06255"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRL29854.1"
                     /translation="MGSPPHHGPAAPPLWRQLQVAARVVQGVRQGRSLSTQLSRVEPA
                     LRPGAQALSFHALRWLGLAEALRGQLARRAPPPAADALLCTSLALAQPEAQAPYEVFT
                     LVDQAVEAAKRDAALRPQAAFINACLRRFLRERAALMEAVAQDPVARWNHPPWWIERL
                     QRDHPAHWAAILATAQQPAPMDLRVNLQRTTVADFCARLAAHGIGAGALGGTAVRLQR
                     PQAVTDIPGFSDGHASVQSATAQRAASLLLGDSMDGAPLVLDACAAPGGKTAHLLEHC
                     PHARVVALEIDPARSQRIGENLMRLGLQADLRVADAADVTSWWRGELFDAILLDAPCS
                     ASGIVSRHPDVRWLRRASDVAQLAAQQDRLLRALWPLLQPGGRLLYCTCSVFLEEGQD
                     RIQTFL"
     assembly_gap    3453..3552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3553..3987
                     /locus_tag="PKV30_06260"
     CDS             <3553..3987
                     /locus_tag="PKV30_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HRL29855.1"
                     /translation="VEDALHKGIPVHFVAEADVMRERWYWYDRNVASAQRYMRVAYQP
                     LTRRWRLNTSSEPIVNSGLGVSLTQNYDSLSEVMAAVQRIGRWKIASAGDLESGGRYT
                     LRFRFRLDASQLPRTLQLGTVGQSDWAVSVERRIDLTQELAR"
     gene            3984..6290
                     /locus_tag="PKV30_06265"
     CDS             3984..6290
                     /locus_tag="PKV30_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL29856.1"
                     /translation="MSGAEPALQVQSDTPHKRRARWALWVAIALLVTIGLVLVFLLAQ
                     ATNNRLLYERYYTRLFAINVAVAGVLALVIGWGIYRLLARLRRGKFGSRLLIKLAAIF
                     GLVGVLPGLLIYVVSYQFVSRSIESWFDVRVEGALESGLTLARTSLDTQARDFGNQLR
                     AGSATLAETPEALVALPLDRLRTQLGASDVTLWAASGRLIASAGQSRYQLTPERPSSQ
                     MLRQLRAQGFATQIEGLDEPAPAGSVPEARIRALVLVSARSTGLLGEQWLLQAVQPLP
                     TALVTHALAVQEANREYQERALGRDGLRRMYIGTLTLSLFLAVFGAVLLAVVLGKQLA
                     TPLLMLADGVRQVAAGDLTPKVILPGRDELVGLTRSFADMTQQLADARAAVDSSMREV
                     DAARAHLQTILDNLTAGVIVLDRHGAIQSANPGATRILRAPLAASTGRPLGSLPALEG
                     FAADVSTQFSEFEGERLEHGLENWQKSFELYGQGGGSLANDAVTLIARGAALPGGQRL
                     LVFDDISEIVSAQRAQAWGEVARRLAHEIKNPLTPIQLSAERLEHKLADKLPAADRAL
                     LTKSVRTIVDQVDAMKRLVNEFRDYARLPSAELKPVDLNALVSEVLLLYGDAGEAGQN
                     SHVPVRFECDDACPPILGDAQQLRQVIHNLVQNAIDASEGQIRAGAQAQVVVRTQWLD
                     ALQRVRFSVVDSGHGFSDAILKRAFEPYVTTKAKGTGLGLAVVKKIAEEHGARIDVSN
                     RIIDGEICGAQVSLSFQVASMAAGASSG"
     gene            6314..7024
                     /locus_tag="PKV30_06270"
     CDS             6314..7024
                     /locus_tag="PKV30_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL29857.1"
                     /translation="MANILVVDDELGIRDLLSEILNDEGHTVELAENAAQARAARQGA
                     RPDLVLLDIWMPDTDGVTLLKEWRGTGLLTMPVIMMSGHATIDTAVEATRIGAQSFLE
                     KPITLQKLLKAVDQALARDVPQRKSPADGLEINAPAPMSPVAELTVESAMMGGQTLPT
                     AVDLSPQGQQTFDLDKPLRDARDAFEKAYFEFHLAKEGGSMTRVAEKTGLERTHLYRK
                     LKQLGVDLSRGRRSAVVS"
     gene            7059..7134
                     /locus_tag="PKV30_06275"
     tRNA            7059..7134
                     /locus_tag="PKV30_06275"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7092..7094,aa:Phe,seq:gaa)
     gene            complement(7208..>7955)
                     /locus_tag="PKV30_06280"
     CDS             complement(7208..>7955)
                     /locus_tag="PKV30_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577238.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRL29858.1"
                     /translation="TFWREFGAVLKEGLGEDHANRDRIAKLLRYASTTTDAQTVSLAD
                     YKARMKDGQKAIYYITADTLAAAKNSPQLEVFKKKGIEVLLMTDRVDEWSLSYLREFD
                     GTPLQSVAKGAVDLGELQDEAEKKAFDAAAEHVKPLLEKLKEALKDQVEDVRVTTRLV
                     DSPACLVVKDAGMSMQLARMLKQAGQPVPDSKPVLEVNPEHPLVKKMESHPPHFDDLA
                     HILFDQALLSEGGLPEDPAAYVRRVNALLA"
BASE COUNT         1302 a         2528 c         2679 g         1346 t
ORIGIN      
        1 tgcgcaccgc gctgacctgc gccacgcgct tcggcatctg cgccaagtgc tacggacgcg
       61 acctgggtcg cggcggtctg gtgaaccagg gcgaggccgt gggcgtgatt gccgcgcagt
      121 cgatcggcga gccgggcacg cagctgacga tgcgcacctt ccacatcggt ggcgccgcat
      181 cgcgcgccgc cgtggcgtcg agcgtggaag ccaaatccaa cggcgtgatc ggcttcaacc
      241 cgaccatgcg ctacgtcacc aacagcaagg gcgaactggt cgtgatttca cgctccggcg
      301 agatcgtgct gcacgacgag cacggccgcg agcgcgagcg ccacaaggtg ccctacggcg
      361 ccacgctgac ggtgaaggcc gaccagcaga tcaaggccgg caccatcctg gccaactggg
      421 atccgctgac gcgccccatc atcaccgagt tcgccggtca ggtgaggttc gagaacgtcg
      481 aggaaggtct gacggtcgcc aagcaggtgg acgaggtcac gggcctgtcc acgctggtgg
      541 tgatcgatcc caagcgccgc ggctcggcca agatcgtgcg tccgcaggtc aaactggtgg
      601 atgcgggcgg caatgaggtc aagatccccg gcaccgatca ctcggtgacg attggcttcc
      661 aggtcggcgc gctgatccag gtgcgcgacg ggcaggacgt gggccccggc gaggtgctgg
      721 cgcgcattcc ggtcgaaggc cagaagacgc gcgacatcac gggcggcttg ccgcgcgtgg
      781 ccgagctgtt cgaggcacgt tcgcccaagg acaagggcac gctggccgag ctgaccggca
      841 ccgtgtcgtt cggcaaggag accaagggca agattcgcct gcagattacc gaccccgatg
      901 gcaaggtgtg ggaagagctg atccccaagg aaaagaacat cctggtgcac gaaggccagg
      961 tggtcaacaa gggcgagagc atcgtcgacg gcccggccga cccgcaggac atcctgcgcc
     1021 tcttgggcat ggaagaactg gcgcgctaca tcgtcgacga agtgcaggac gtctaccggc
     1081 tgcagggcgt gaagatcaac gacaagcaca tcgaggtcat cgtgcgccag atgctgcgcc
     1141 gcgtggtggt cgagaacccg ggcgattccg gctacatcgc cggcgagcag gtcgagcgca
     1201 gcgaaatgct ggacaccaac gacgcgctgc gcgccgaagg caagctgccc gcgacctaca
     1261 ccaacctgct gctgggcatc accaaggcca gcctgtccac cgacagcttc atcagcgccg
     1321 cgtcgttcca ggaaaccacg cgcgtgctga ccgaagccgc catcatgggc aagcgcgacg
     1381 agctgcgtgg cctgaaggag aacgtcatcg ttggccgcct gatccccgcg ggcaccggcc
     1441 tggcctatca ccaggcacgc aaggtgaagg accagatgga cgaggccgag cgccgcgcca
     1501 tcgccgatgc cgaagcggcc gaactggcgg gtgaagtgga agtcgtcgaa ggcggcgaat
     1561 ccgccagtgt cgatgagggc gcttcggccg attgagctgt tgctgcgttg ttgatagcgc
     1621 cctctccttg ctgaacaagc gggagagggc gttttgtttg catgaacgat gtttgattca
     1681 agctggtggc tgggcctggc ggcggcgctg ctgttcttct ggggcgtggg tggatacaac
     1741 cgcctggtgc ggctgcgcgc ggccgtgacc agcgcctttg cggcgctgga cgagcaactg
     1801 gtgcgccagc tggtgtgggt gcaagggtgc ctgcccgatg ccatgcgcgg cggcgtgccc
     1861 acgttgccgg gcgagctgca ggacgacgtc accgccgctt gggcacgcct gcaagccgcc
     1921 agcgagcagt tcgccgtcgc gctggcccat gcgcgggcgc agccgatcga cgtgcagcgc
     1981 atggccagtc tggtgatggc gcacgaggcg ctgcgcaccg cgtggggcgc cacgctggcg
     2041 gaagcggtgc ctgccgacgc cgtgccatcg tcggagcgtt tgcaagcgcg ttggttgcgc
     2101 ttgctgcacc agtcgctgcc gctgcgcacc gccttcaacg atgctgccca aagctacaac
     2161 caggccatcg cgcagtttcc agcctcgctg ctggcgcgct tgttcggctt caagccggcg
     2221 ggaacggtga cccgcctcgc cgatgggcgc tgacgccatg ggttcaccgc cccatcacgg
     2281 cccggcagcg ccgcctttgt ggcgccaact ccaggtcgcc gctcgtgtgg tgcagggggt
     2341 ccggcaaggc cgctcgctgt caacacaact gagtcgtgtg gagccggccc tgcgcccagg
     2401 tgcgcaggca ctgtcgtttc acgcgttgcg ctggctcggt ctggccgagg ccttgcgcgg
     2461 gcaactggcg cgccgcgcgc cgccccctgc tgccgatgca cttttgtgca ccagcctggc
     2521 gctggcgcag cccgaggcgc aggcgcccta cgaggtgttc acgctggtcg atcaggcggt
     2581 ggaagcggcc aaacgggatg cggcgctgcg gccacaggcg gccttcatca acgcctgcct
     2641 gcgccgattt ctgcgcgagc gggctgcgtt gatggaggcc gtggcgcagg acccggtggc
     2701 gcgctggaac catccgccgt ggtggatcga acgcttgcag cgcgatcatc cggcgcactg
     2761 ggccgccata ctggcgacgg cccagcagcc tgcgccgatg gatttgcgcg tcaatctgca
     2821 acgcaccacc gtggcggatt tctgcgcgcg cctggcagcg cacggtatcg gcgccggcgc
     2881 gctcggcggc accgccgtgc gtctgcagcg gccacaggcg gtgacggaca ttcccggctt
     2941 ttccgacggc catgcgtccg tccaatccgc gacggcgcag cgcgccgcca gcctgctact
     3001 gggcgacagc atggacggcg cgccgctggt gctggacgca tgtgcggcac cgggcggcaa
     3061 gacggcgcat ctgctcgagc attgcccgca cgctcgcgtc gtcgcactcg agatcgaccc
     3121 cgcacgcagt cagcgcatcg gcgagaacct gatgcgcctg ggcttgcagg ccgatctgcg
     3181 cgtggccgat gcggccgacg tgacctcgtg gtggcgcggc gaactgttcg acgccatcct
     3241 gctcgacgcg ccttgcagcg cctcaggcat cgtgagccgc cacccggacg tgcgctggct
     3301 gcgtcgggca tccgatgtgg cgcagctggc ggcgcagcaa gatcgcttgc tgcgggcgct
     3361 ctggccgctg ctccaacccg gtggacgcct gctgtactgc acgtgctcgg tgtttctcga
     3421 agaaggtcag gaccgcatcc aaacgtttct tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nngtcgaaga cgccctgcac aagggcattc cggtccattt cgtcgccgag
     3601 gcggacgtga tgcgcgaacg ctggtactgg tacgaccgca acgtcgcctc ggcgcagcgc
     3661 tacatgcggg tcgcctacca gccgctgaca cggcgctggc ggctcaacac ctcatcagaa
     3721 ccgatcgtca acagcggcct gggcgtcagc ctgacgcaga actacgattc cttgtccgaa
     3781 gtgatggccg ccgtacagcg catcgggcgc tggaagatcg ccagcgcagg cgatctggag
     3841 agtggtgggc gctacaccct gcgctttcgc ttccggctcg atgccagcca gttgccgcgc
     3901 accctgcaac tgggcaccgt tggccagtcg gattgggcgg tgtcggttga acgccgcatc
     3961 gacctgacgc aggagttggc ccggtgagcg gcgccgaacc cgcgctgcag gtccagagcg
     4021 acacgccgca caagcgcaga gcgcgctggg cgctgtgggt ggccatcgcg ctgctggtga
     4081 ccatcgggct ggtgctggtg tttctgctgg cccaagccac caacaaccgc ctgctttacg
     4141 agcgctacta cacccgcctg tttgccatca acgtggcggt ggccggcgtg ctggcgctgg
     4201 tgatcggctg gggcatttac cggctgctgg cgcgcttgcg gcgcggcaag ttcggcagtc
     4261 gtttgctgat caagctggcg gcgatcttcg ggcttgtggg ggtgttgccg gggctgctga
     4321 tctacgtcgt gtcgtatcag ttcgtatcgc gttccattga atcgtggttc gatgtaaggg
     4381 tggagggcgc gttggagtcc ggcctgacgc tggcccgaac atcgctcgac acgcaggcgc
     4441 gcgacttcgg caaccaattg cgcgccggct cagccacgct ggctgaaacg ccggaggcgc
     4501 tggtcgcgct gccgctcgat cgtctgcgca cgcaactcgg ggcttccgat gtcacgctgt
     4561 gggcggcgtc gggccgactg atcgccagcg cgggccagtc gcgctatcag ttgacgccgg
     4621 agcggcccag ttcgcagatg ctgcgccagt tgcgtgcgca aggctttgcc acccagatcg
     4681 aaggcctgga cgagcccgcg cccgccggca gcgtgcctga ggcgcgcatc cgggcgctgg
     4741 tgctggtcag tgcccgttca acgggcctgc tgggcgagca atggttgctg caagcggtgc
     4801 agccgctgcc caccgcgctg gtcacgcatg cgctggcggt gcaggaggcc aaccgcgaat
     4861 accaggagcg cgcgctgggt cgcgatgggc tgcggcgcat gtacatcggc accctcacgc
     4921 tcagcctgtt tctggccgtg ttcggcgcgg tgctgctggc ggtggtgctg ggcaagcaac
     4981 tggccacgcc gctgctgatg ctggccgacg gcgtgcggca agtggccgca ggcgacctga
     5041 cgcccaaggt cattttgccg gggcgcgatg agttggtcgg cctgacgcgc tcgtttgccg
     5101 acatgacgca gcagctggcc gatgcgcgcg cggccgtcga ttcgagcatg cgcgaggtcg
     5161 atgcggcgcg tgcgcacctg caaaccattc tcgacaacct gacggccggc gtgatcgtgc
     5221 tggaccgtca tggcgccatt cagtccgcca atcccggcgc cacccgaatc ctgcgcgcgc
     5281 cgctggcggc gagcaccggc cggccgttgg gcagcctgcc ggcgctggag ggatttgctg
     5341 ccgacgtgtc aacacagttt tccgagttcg agggtgagcg gctggagcat ggactggaga
     5401 actggcaaaa gtcgttcgag ctctacggcc agggcggcgg cagcctcgcc aacgatgccg
     5461 tgacgctgat cgcccgtggt gccgcgctgc ccggtgggca gcggctgctg gtgttcgacg
     5521 atatttccga gatcgtttcg gcacagcgcg cgcaggcttg gggcgaggtg gccaggcggc
     5581 tggcgcacga gatcaagaat cccctgacgc ccatccagtt gtcggccgaa cggctggagc
     5641 acaagctggc ggacaaattg ccggcggccg accgggcatt gctgaccaag tcggtgcgca
     5701 ccatcgtcga tcaggtggac gcgatgaagc gtctggtcaa cgagttccgt gactacgcac
     5761 gcctgccctc ggccgaactg aagccggtcg accttaacgc gctcgtcagt gaagtgctgc
     5821 tgctgtacgg cgacgcaggc gaggcgggtc agaattcgca tgtccccgtg cggttcgaat
     5881 gcgatgacgc ctgtccgccg atcctgggcg acgcgcagca attgcggcag gtcattcaca
     5941 acctggttca gaacgcgatc gacgcgtcag aggggcagat acgcgctggc gcgcaggcgc
     6001 aggtggtggt gcgcacccag tggctcgacg ccttgcagcg cgtgcgcttc agcgtggtcg
     6061 acagcgggca tggtttttcc gacgcgatcc tcaagcgggc gtttgaaccc tacgtcacca
     6121 ccaaggcaaa gggcaccggg ctcggtctgg cggtcgtcaa gaaaatcgcc gaagagcatg
     6181 gcgcccgcat cgatgtatcc aatcgcatca tcgatggcga gatttgtggc gcgcaagtat
     6241 cgttatcatt ccaagtggca tccatggcgg caggcgcttc ttctggctga agccaatcac
     6301 acccaacaat cctatggcga acattctggt ggtcgacgat gaacttggca ttcgggacct
     6361 cttgtccgag atcctcaatg acgaaggcca caccgtggag cttgccgaaa acgccgccca
     6421 ggcacgcgcc gcgcggcaag gcgcgcgtcc tgacctggtg ctgctggaca tctggatgcc
     6481 tgacacggac ggcgtgacgc tgctcaagga atggcgcggc accggcctgc tcaccatgcc
     6541 ggtcatcatg atgagcggtc atgccaccat cgacacggcg gtcgaagcga cccgcatcgg
     6601 cgcacagtcg tttcttgaaa aaccgatcac gctgcaaaag ctgttgaagg cggtggatca
     6661 ggcgcttgcg cgcgacgtgc cgcagcgcaa gtcgcctgcc gatggactgg aaatcaacgc
     6721 tccggcaccg atgagcccgg tcgctgaact gacggtcgaa tccgccatga tggggggtca
     6781 gaccttgccc acggcggtcg acctcagccc gcaaggccag cagacgtttg atctggacaa
     6841 accgctgcgc gatgcgcgcg atgcgttcga aaaagcgtat ttcgagttcc acttggccaa
     6901 ggaaggcggc tccatgacgc gggtagcgga aaagaccggc ctggagcgca cacacctgta
     6961 tcgcaagctg aaacaactcg gcgttgacct gtcgcgcggg cgtcgcagcg ccgtcgtctc
     7021 gtaacgatct ggagcagtgc tagaatagaa ggcttaatgg cccggtagct cagttggtag
     7081 agcagcggat tgaaaatccg cgtgtcggtg gttcgattcc gccccaggcc accaaaccaa
     7141 aaaaaagcca acctgcacag gttggctttt ttcgtcttgc gccgccaggg cgcaagtcac
     7201 atggctctta tgccagcagc gcgttcaccc tgcgcacata cgccgccggg tcttcaggca
     7261 aaccgccttc cgacagcagc gcctgatcaa acaggatgtg cgccaggtcg tcgaaatgcg
     7321 gcgggtggct ttccattttt ttcaccagcg ggtgttccgg attcacctcc agcacgggct
     7381 tgctgtcggg cacgggctga ccggcctgct tcagcatgcg ggccagttgc atgctcatgc
     7441 cggcgtcctt caccaccagg caggcgggcg aatcgaccag gcgcgtggtg acgcgcacgt
     7501 cttccacctg atccttcaag gcttctttca gcttctccag cagcggcttg acgtgctccg
     7561 ccgctgcgtc aaaggccttc ttctcggcct cgtcctgcaa ctcgccaaga tccaccgcgc
     7621 ccttggcgac cgattgcagc ggcgtgccgt cgaactcgcg caggtaagac agcgaccact
     7681 cgtccacgcg gtcggtcatc agcagcacct cgatgccctt tttcttgaag acttcgagct
     7741 gcgggctgtt cttggccgcc gccagcgtgt cggcggtgat gtagtaaatg gccttctggc
     7801 cgtctttcat gcgcgccttg tagtcggcca gtgagacggt ctgtgcgtcg gtggtggtcg
     7861 acgcgtagcg tagcagcttg gcgatgcggt cgcggttggc gtggtcttcg cccaggcctt
     7921 ccttcagcac ggcgccgaac tcgcgccaga aggtg
//
LOCUS       DAOMVO010000095         7888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2391_length_7888_cov_13.327716, whole genome shotgun sequence.
ACCESSION   DAOMVO010000095 DAOMVO010000000
VERSION     DAOMVO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2391_length_7888_cov_13.327716"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /gene="kdsA"
                     /locus_tag="PKV30_06285"
     CDS             complement(<1..565)
                     /gene="kdsA"
                     /locus_tag="PKV30_06285"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRL29859.1"
                     /translation="MKLCGFDVGLSQPFFLIAGPCVVESEQLQMDVAGQLKEITAGLG
                     IPFIFKSSYDKANRSSGTSFRGPGMDKGVEILAKVKRELNVPLLTDVHTEAEIPAVAQ
                     VVDVLQTPAFLCRQTDFIRAVAQSGKPVNIKKGQFLAPHDMKNVIDKARAAAREAGLP
                     EDSFMACERGASFGYNNLVSDMRSLAIM"
     gene            complement(562..2247)
                     /locus_tag="PKV30_06290"
     CDS             complement(562..2247)
                     /locus_tag="PKV30_06290"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRL29860.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFITTRMKKANNFTTGQIYKSVLEKE
                     RRGDYLGKTVQVIPHVTNEIQDFIKRGAGFGTAGAVDVAIVEIGGTVGDIESLPFLEA
                     VRQLSLKHGPNNSAFVHLTYLPWIAAAGELKTKPTQHTVQKLREIGIQPDALLCRADR
                     PIPEEEREKISLFTNVAEWGVISMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPAN
                     LKRWDALVHEVEHPQHEVRIAMVGKYVELSDSYKSVNEALRHAGMHNHARVKIDHIDS
                     ETITPASVAQLAQYDAILVPGGFGSRGVEGKIATARYAREHQVPYLGICLGMQVATIE
                     YARHVAGLADANSTEFDPATPHPVIALITEWKDKDGSVQKRDAKSDLGGTMRLGAQSA
                     DVQAGTLAHRIYGDVVTERHRHRYEANVNYLDKLRDAGLVISALTQREHLTEIVELPQ
                     GTHPWFIGVQFHPEFKSTPWDGHPLFNGFIEAALAHQAARAGHDAAKTLKAVK"
     gene            2462..3703
                     /gene="coaBC"
                     /locus_tag="PKV30_06295"
     CDS             2462..3703
                     /gene="coaBC"
                     /locus_tag="PKV30_06295"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRL29861.1"
                     /translation="MNAADTSGELAHRHIVLGLSGGAACFKAAEFCRALVKAGATVQV
                     VMTEAACQFITPVAMQALSGRPVYVSQWDARPGNNMAHINLSREADAIFVAPASADFI
                     AQLAQGRAGELLPLLCLARPIARVPLLIGPAMNREMWAHPATQRNLRQIAEDGAVVLG
                     VARGDQACGEFGHGRMLEASELLEELIAYLAPKPLLGQRVLVTAGPTFEAIDPVRGIT
                     NLSSGKMGFAIARAAREAGADVTLIAGPVHLPTPRGVQRIDVDSARNMHAAAVQQAQA
                     AHIFIATAAVADWRPATQAEHKIKKDGSGEAPQMSFVENPDILAEIAHSPRAQSGELY
                     CVGFAAESENLEANAQAKRARKGVPLLVANIGPATFGRDDNALLLVDDEGVLELPHAS
                     KRVLAQQLIAEIARRVSGLEA"
     gene            3700..4146
                     /gene="dut"
                     /locus_tag="PKV30_06300"
     CDS             3700..4146
                     /gene="dut"
                     /locus_tag="PKV30_06300"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRL29862.1"
                     /translation="MNLDVMILDERLRAQMPAYATPGSAGMDLRACLDAPLTLPPGAW
                     QLVPTGMAIHLADPGYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSP
                     TAFTIEPMERIAQLLIVPVAQATFRVVDSFEASARGTSGYGSTGRG"
     gene            4298..4771
                     /locus_tag="PKV30_06305"
     CDS             4298..4771
                     /locus_tag="PKV30_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRL29863.1"
                     /translation="MQISQRLVSSLAVVTAAAALVACAAPGPHSNQYPNTPTTSYPSN
                     PGYAQFGRVTNVEYVRGGQSQGIAGAVVGAAVGGLAGNQVGGGSGRTAATVVGVVGGA
                     LIGRALEQNMNRGTVDHYRVTVQFDNGGTRQFDYAQAPNVQIGDRVRADGDQLYR"
     gene            complement(4841..5365)
                     /locus_tag="PKV30_06310"
     CDS             complement(4841..5365)
                     /locus_tag="PKV30_06310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRL29864.1"
                     /translation="MEISPQSVVALTWTLKDSLADTLDELQEPVEFLVGGDDLLPAIS
                     AALQGHEAGATLDLNLEPEDAFGDFDEELVFLAKRDQLPDGLEEGMLLDAAALPADIA
                     SEAPSDAIFTITEIYPEHVVLDANHPLSGISLRLHLKVVAVRAATEEELERGSVGTGF
                     FRLSPMAPGSPHVH"
     gene            5422..6546
                     /locus_tag="PKV30_06315"
     CDS             5422..6546
                     /locus_tag="PKV30_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRL29865.1"
                     /translation="MDVDQPLPLLGGLSPARFMQRHWQKKPLLVRQAVAGIKPLLSRA
                     ELFALAADAAVESRLIVRGADGWQLKRGPLARRALPPLAQRDWTLLVQGVDLHDERAH
                     ALLQQFRFVPDARLDDLMISYASDGGGVGPHFDSYDVFLLQTRGRRRWRIGRQKNLSL
                     VEGLPLKILADFQPEQEHVLEPGDMLYLPPRWAHDGVAEGECMTYSVGFRQPSTDEVV
                     RELLQRVADDANDLVGDAAYRDPDQPATDQPGQLPPAMLEFARSAIEKALNDPRHLSL
                     LLGEWLTEPKPSVWFDAGDGAGDASPLQVVLDRRTRMLYAAQHVFINGEGFSASGQDA
                     RLMRTLADQRRLTAAQCARLSAGARDLLSQWQAAGWLHER"
     gene            6536..7042
                     /locus_tag="PKV30_06320"
     CDS             6536..7042
                     /locus_tag="PKV30_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29866.1"
                     /translation="MNDDTSSAGELPSGTFIGRDAFRERVRAGLSVAAAEGWAELVLC
                     DADFHDWPLGERAVVEALTAWVRRAQRLVMLAKTYDEVPRQHAQFVEWRRLWSHKIEC
                     RQCKEADASALPSALWSPVWAVQRLDRLHSNGVCGTDPGRRKALREEIDGWLARSTSG
                     FPAYTLGL"
     gene            7229..7639
                     /locus_tag="PKV30_06325"
     CDS             7229..7639
                     /locus_tag="PKV30_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29867.1"
                     /translation="MNKSLVLAALIAAASLAACGKKEEPAPAPAPAAAEAANAAEQAA
                     SAADAAKDAAATAAGAAMSEAASAAGAAASATGAAVAAGADAAGAAANAAAAQAATAA
                     TTAATAAAAGATNAAAGAANAAADAANAAASAAK"
BASE COUNT         1245 a         2546 c         2672 g         1425 t
ORIGIN      
        1 gcatgatggc caggctgcgc atgtcgctga ccaggttgtt gtagccaaag ctggcgccgc
       61 gctcgcaggc catgaagctg tcttcgggca ggcctgcctc gcgcgcggcg gcgcgggcct
      121 tgtcgatgac gttcttcatg tcgtggggcg cgaggaactg gcccttcttg atgttgaccg
      181 gcttgcccga ctgcgccacc gcgcggataa agtcggtctg gcggcacaga aaggcgggcg
      241 tctgcagcac atcgaccact tgcgcgacgg cgggaatttc ggcctcggtg tgtacgtcgg
      301 tcagcagggg cacgttcagt tcgcgcttga ccttggcgag gatttcaacg cccttgtcca
      361 tgcccgggcc gcgaaagctg gtgcccgacg agcggttggc cttgtcgtag ctgcttttga
      421 agatgaaggg gatgccaagg ccggccgtga tttctttcag ttgcccggcc acgtccatct
      481 gcagctgttc gctctcgacc acgcaggggc cggcaatcag aaagaaaggc tggctcagcc
      541 cgacgtcgaa gccgcacaac ttcatttgac cgccttcagt gtcttggccg cgtcgtggcc
      601 ggcgcgcgcc gcttggtgcg ccagtgccgc ctcgatgaag ccgttgaaca gcgggtggcc
      661 atcccacggc gtgctcttga actccgggtg aaactgcacg ccgatgaacc acgggtgcgt
      721 gccttgcggc agctcgacga tctcggtcag gtgctcgcgc tgcgtgaggg cagagatcac
      781 caggcccgca tcgcgcaatt tgtccaggta gttgacgttg gcctcgtagc ggtggcggtg
      841 gcgctcggtc accacgtcgc cgtagatgcg gtgcgccagc gtgccggcct gcacgtcggc
      901 gctttgcgcg cccaggcgca tggttccccc aaggtcggac ttcgcatcgc gcttttgcac
      961 gctgccgtcc ttgtccttcc actcggtgat gagcgcgatg acggggtgcg gcgtggcggg
     1021 gtcgaactcg gtgctgttgg cgtcggccag gccagcgacg tggcgcgcgt actcaatggt
     1081 ggcgacctgc atgcccaagc aaatgcccaa gtacggcacc tggtgctcgc gcgcatagcg
     1141 ggcggtggcg atcttgccct cgaccccgcg cgaaccgaaa ccgcccggca ccagaatggc
     1201 gtcgtactgc gccagttggg ccacgctggc gggcgtgatg gtttccgaat cgatgtggtc
     1261 gatcttcacg cgcgcgtggt tgtgcatgcc ggcgtggcgc agcgcctcgt tgaccgactt
     1321 gtagctgtcg gacagctcga cgtacttgcc caccatggcg atgcgcacct cgtgctgcgg
     1381 gtgctccacc tcgtgcacca gcgcgtccca gcgcttcagg ttggcgggcg gcgtgttcag
     1441 gcgcagcttg tcgcagatca ggccgtccag cccttgctcg tgcagcatgc gcggcacctt
     1501 gtaaatggtg tccacgtccc acatgctgat gacgccccat tcggccacgt tggtgaacag
     1561 gctgatcttt tcgcgctctt cctcggggat cgggcggtcg gcgcggcaga gcagggcgtc
     1621 gggctggatg ccgatttcgc gcagcttttg cacggtgtgc tgggtcggct tggttttcag
     1681 ctcgcccgcc gccgcgatcc acggcaggta ggtcaggtgc acgaaggccg agttgttcgg
     1741 cccgtgcttc aagctgagct ggcgcaccgc ttccagaaac ggcagcgatt cgatgtcgcc
     1801 caccgtgccg ccgatctcga caatcgccac gtccaccgcg ccggccgtgc caaaaccggc
     1861 gccgcgcttg atgaagtcct ggatctcgtt ggtgacgtgc ggaatcacct gcaccgtctt
     1921 gccgaggtag tcgccgcggc gctctttttc gagcacgctc ttgtagatct ggccggtggt
     1981 gaagttgttg gccttcttca tccgcgtggt gatgaagcgc tcgtaatggc ccagatcgag
     2041 gtcggtttcg gcgccatcgt cggtgacgaa cacctcgccg tgctgaaacg gcgacatggt
     2101 gcccggatcg acgttgatgt acggatcgag cttgatgagg gtgactttga ggccgcgcga
     2161 ttcgaggatc gcggccaggg aggccgaggc gattcccttg cccagggaag acaccacacc
     2221 gccggtgacg aagacgaatt tggtcatgtc aggacgaagg cgcaatacct tcgctcggtg
     2281 ggaaatttcg attatagatg tcgccccggg acacggcagc attgatccga gtgcttcgat
     2341 ttcttgtggt ttactatcga ataaatagct gctcgcgctt gtctgtcaag cgccagcgcc
     2401 tgttttgact ctgaaaattc gcccgaccgc cagccattca gcctctgcta cattgcccgc
     2461 catgaacgca gcggacacat cgggcgaact ggcccatcgc cacatcgtgc tgggcctgtc
     2521 gggcggcgcg gcgtgcttca aggcggcgga attttgccgc gcgctcgtca aggccggcgc
     2581 caccgtgcag gtggtgatga ccgaggcggc ctgccagttc atcacgccgg tggccatgca
     2641 ggcgctgtcg gggcggccgg tgtacgtgtc gcaatgggat gcgcggccgg gcaacaacat
     2701 ggcgcacatc aatttgagcc gcgaggccga cgccatcttc gtcgcgcccg ccagcgccga
     2761 cttcatcgcg caactcgcgc agggcagggc gggcgagctg ctgccgttgc tgtgcctggc
     2821 gcggcccatc gcgcgcgtgc ccttgctgat cgggccggcc atgaaccgcg agatgtgggc
     2881 gcaccccgcc acgcagcgca acctgcgcca gattgccgag gacggcgcgg tggtgctggg
     2941 cgtggcccgg ggtgaccagg cgtgcggcga attcggccac ggccgcatgc tggaggcgag
     3001 cgagttgctg gaagagctga tcgcctacct ggcgcccaag ccgctgctcg gccagcgcgt
     3061 gctggtcacc gccgggccga cgtttgaggc catcgacccg gtgcgtggca tcaccaacct
     3121 gtcgtccggc aagatgggtt tcgccattgc ccgcgccgcg cgcgaggcgg gcgccgacgt
     3181 gacgctgatc gccggcccgg tgcatctgcc gaccccacgc ggcgtgcagc gcatcgacgt
     3241 ggattcagcg cgaaacatgc acgcagcagc cgtccaacaa gcgcaggcag ctcacatctt
     3301 catagcaacg gcagcggtcg ccgattggcg acccgccacg caggcagagc acaagatcaa
     3361 gaaagatggc tctggcgagg cgccgcaaat gtcgtttgtc gagaacccgg acatcctggc
     3421 cgagatcgcg cattcgccgc gcgcgcaatc gggcgagctg tattgcgtcg gctttgccgc
     3481 cgagagcgaa aacctggaag ccaacgcgca agccaagcga gcgcgcaagg gcgtgccgct
     3541 gctggtggcc aacatcggcc ccgccacgtt tggcagggac gacaacgcgc tgctgctggt
     3601 cgatgacgag ggtgtgcttg agttgccgca cgccagcaaa cgcgtgctgg cgcagcaact
     3661 gatcgccgag attgcgcggc gcgtgtcggg gttggaggca tgaacctcga cgtgatgatc
     3721 ctcgacgagc gcctgcgcgc gcaaatgccg gcttacgcta ccccgggcag cgcggggatg
     3781 gatttgcgcg cctgcctcga tgcgccgttg acgctgccgc ccggcgcctg gcaactggtg
     3841 cccaccggca tggcgattca cctggccgac cccggctatg cggcgctgat cctgccgcgc
     3901 tcgggattgg gtcacaagca cggcatcgtg ctgggcaatc tggtgggctt gatcgacagc
     3961 gattaccagg gccagctgat ggtgagcgcc tggaaccgca gccccaccgc gttcaccatc
     4021 gagcccatgg agcgcatcgc gcaactgctc atcgtgcccg tcgcacaggc gacgttcagg
     4081 gtggttgatt cgttcgaggc ctcggcgcgc ggcaccagcg gctatggctc aaccggccgc
     4141 gggtaggcct gttcctacgc ggacagcgct gtgtcgctga caagggcaag cgcacgaggc
     4201 gcgtggcgtt cgtgccgctg ggtcgttcaa atatccacgt gatgcgttaa tcacccaggc
     4261 gttcagatgg atgcctgaat cttcaggaga gaatcaaatg caaatttccc aacgtctggt
     4321 gtcttccctt gccgtggtta ccgcagccgc cgcgctggtg gcgtgcgccg cgccaggccc
     4381 gcactccaac caatacccca acaccccgac gaccagctac ccgtcgaacc ctggctacgc
     4441 gcagtttggc cgcgtgacca acgtcgagta cgtgcgcggc gggcagtcgc aagggattgc
     4501 tggtgccgtc gtcggcgctg ccgtgggcgg tctggccggc aaccaggtgg gcggtggctc
     4561 gggccgtacc gctgccacgg tggtcggtgt ggttggcggc gcgttgattg gccgcgcgct
     4621 ggagcagaac atgaaccgcg gcaccgtcga ccactatcgc gtcactgtgc agttcgacaa
     4681 cggcggcacg cgccagttcg actacgcgca ggcgcccaac gtgcagattg gcgaccgcgt
     4741 gcgcgccgat ggcgatcagc tgtaccgctg aaatcggccg ccatgggccc ccatgaaaaa
     4801 cggcaccgaa gtgtgccgtt tttctttgcc gatcaagcca tcaatggacg tgcgggctgc
     4861 caggcgccat cgggctcagc cggaagaacc ccgtgccgac gctgccgcgc tccagctctt
     4921 cttcagtcgc cgcgcgcacc gccacgacct tcaaatgcag gcgcaaagag atgcctgaca
     4981 gcgggtggtt ggcgtccagc accacgtgct ccgggtagat ctcggtgatg gtgaagatcg
     5041 cgtcgctcgg cgcctcgctg gcaatgtcgg ccggcaaggc cgctgcatcg agcagcatgc
     5101 cttcctccag gccgtctggc agttggtccc gcttggccag aaagaccagc tcctcgtcga
     5161 agtcgccgaa ggcgtcttcc ggctccaggt tcaggtcgag cgtggcgccc gcttcgtggc
     5221 cttgcagcgc ggcgctgatg gcgggcagca gatcgtcgcc gccgaccaag aattcaacgg
     5281 gctcctgcaa ctcgtccagc gtatcggcca ggctgtcctt cagggtccag gtgagggcga
     5341 cgacggattg ggggctgatt tccatggcgc cgattgtcgc atcgccggca gcgggtaggc
     5401 ggctttgcgt atgcttgcgc catggatgtc gatcaaccct tgccattgct gggcggcctg
     5461 tcgccggccc gcttcatgca gcgccattgg cagaaaaagc cgttgctggt gcgccaggcc
     5521 gtggcgggca ttaagccgct tttgtcgcgc gccgagctgt tcgcgctggc cgctgacgcg
     5581 gccgtggaat cgcggctgat cgttcgcggc gccgacggct ggcaactcaa acgcgggccg
     5641 ctggcgcgcc gcgccttgcc gccgctggcg caacgcgact ggacgctgct ggtgcagggc
     5701 gtcgatctgc acgacgagcg cgcgcacgcc ttgctgcaac agtttcgctt tgtgccggat
     5761 gcgcgcctcg acgatttgat gatcagctac gccagcgacg gcggcggcgt cggcccgcat
     5821 ttcgacagct acgacgtgtt tttgctgcag acgcgcggcc ggcggcgctg gcgcatcggg
     5881 cggcagaaga acctgagcct ggtcgaaggg ctgccgctga aaattctggc tgatttccag
     5941 cccgagcaag aacacgtgct ggagccgggc gacatgctct acctgccgcc gcgctgggcg
     6001 cacgacggcg tggcagaggg tgaatgcatg acctactcgg tcggctttcg ccaaccgtcg
     6061 accgatgaag tggtgcgcga gttgctccag cgcgtggccg atgacgcgaa cgacctggtg
     6121 ggcgatgccg cgtaccgcga cccggatcag ccggcgaccg accagcccgg gcagttgccg
     6181 ccggccatgc tcgaatttgc acgctcggcg atagaaaagg ccctgaacga tccgaggcac
     6241 ctgtcgctgc tgctgggcga atggttgacc gaacccaagc ccagcgtctg gttcgacgcc
     6301 ggcgatgggg caggtgatgc gtcacccctg caagtggtgc tggatcggcg cacgcgcatg
     6361 ctctacgccg cgcagcacgt gttcatcaac ggcgaaggtt tcagcgccag cggccaggat
     6421 gcgcgcctga tgcggacgct ggccgatcag cgccgcttga ccgctgcgca atgcgcgcgc
     6481 ctgagcgcgg gcgcgcgcga tttgttgagt caatggcaag ctgcagggtg gttgcatgaa
     6541 cgatgacact tcctccgccg gcgaactgcc gtccggcacc ttcatcgggc gcgatgcgtt
     6601 tcgggagcgc gtgcgtgctg gcctgagcgt tgcggcggcc gagggttggg ccgaactggt
     6661 gctgtgcgac gccgattttc acgactggcc gctgggcgag cgggcggtgg tcgaggcgct
     6721 caccgcctgg gttcgccggg cgcagcgcct ggtgatgctg gccaagacct acgacgaggt
     6781 accgcgccag cacgcgcagt tcgtcgaatg gcggcgcctg tggtcgcaca agatcgagtg
     6841 ccgacagtgc aaggaggcgg atgcgtccgc gctgcccagc gcgctctggt cgcccgtgtg
     6901 ggcggtgcag cgcctggacc gactgcacag caacggcgtg tgcggcaccg acccgggccg
     6961 gcgcaaggcg ctgcgcgaag agatcgacgg ctggctggcg cgcagcacca gcgggtttcc
     7021 cgcctacacc ctggggttgt gacagttggt ttgaatcgtg gcgcgggcgc atcagggttt
     7081 atcacgattg cggttacaca agctataatt tctggttgag cggaaagagt ttgagtgctc
     7141 ttctctcaat cagggcgccc ggtgtttgtt gtcatatcag cgtcttggtg ctttccggta
     7201 aaaccaattt tcgacaagga ccattgaaat gaacaaatcg cttgtactgg ccgccctgat
     7261 tgccgccgct tccctggctg cctgcggcaa gaaggaagag ccggcacctg caccggctcc
     7321 ggctgctgct gaagccgcca acgctgctga gcaagccgct tctgccgccg acgccgccaa
     7381 ggacgctgct gccacggccg ctggcgctgc catgagcgaa gccgcctcgg ccgctggtgc
     7441 cgctgcttcc gccaccggcg ctgccgtggc cgccggtgcc gacgccgctg gcgcagctgc
     7501 caacgccgcc gctgcccaag ccgctactgc tgccaccacc gccgcaacgg ctgccgccgc
     7561 tggcgccacc aacgccgctg cgggcgctgc caacgcggcc gccgatgctg ccaacgctgc
     7621 tgccagcgct gccaagtaaa tcaggcgcaa tttccgccag tcgtgcgcat tgccgcgcgg
     7681 cgggtgggaa tcttcaagca gccaccttcg ggtggctttt ttcttggcga tgacggcggc
     7741 tgaccgcgcg atggggtctg tgcgaattca aacgtccagc caaccgcgcc catggcgctc
     7801 ggccagcagc acatcggctt cgccgcatcc cagcaccgct gcaaacgcgc ggctcaccag
     7861 ctcgggtcgg ttgttgcgct cgctcagg
//
LOCUS       DAOMVO010000096         7838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2420_length_7838_cov_16.642940, whole genome shotgun sequence.
ACCESSION   DAOMVO010000096 DAOMVO010000000
VERSION     DAOMVO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_7838_cov_16.642940"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..819
                     /locus_tag="PKV30_06330"
     CDS             169..819
                     /locus_tag="PKV30_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRL29868.1"
                     /translation="MAATKKAAIQLSTPAYWADACTHLAKRDRVMKRLIPRFGDACLQ
                     SRGDAFVTLSRSIVGQQISVKAAQSVWNRFEALPKRLTPAHVLKLKVDDMRAAGLSAR
                     KVEYLVDLALHFDSGRIHVKQWDGMDDEAIIAELVAIRGIGRWTAEMFLMFHLLRPNV
                     LPLDDVGLINGISLNYFSGEPVSRSEAREVAEAWAPWRSVATWYMWRSLEPLPVAY"
     gene            873..1847
                     /locus_tag="PKV30_06335"
     CDS             873..1847
                     /locus_tag="PKV30_06335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRL29869.1"
                     /translation="MAKRTFLDFEQPIAELENKIEELRYVQNESAVDISEEIDQLDKK
                     SLRLTKDIYADLSPWQITKIARHPERPYTLDYVRECFTDFVEMHGDRHFADDQSIVGG
                     LARFNGNACMVIGHQKGRDTKERTLRNFGMVRPEGYRKALRLMKTAEKFKLPVFTFVD
                     TPGAFPGIDAEERGQSEAIGRNIYEMAQLEVPIITTIIGEGGSGGALAIAVADQVLML
                     QYSIYSVISPEGCASILWKTSDKAQDAAEALGITAHRLKALGLVDKIVSEPVGGAHRD
                     HRQMAAFLKRALGEAWRQVSDLKPKELLDRRYERLMSYGRFNDTKAER"
     gene            complement(2116..2406)
                     /locus_tag="PKV30_06340"
     CDS             complement(2116..2406)
                     /locus_tag="PKV30_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HRL29870.1"
                     /translation="MWKLGKLFAMFRKELLLAWAVLRDPRTPKSAKLAVVLAVLYVVS
                     PIDLVSDFIPILGWLDDGLIAYVLLQVAFKFLPPDLHAALRSKVAARTRPVA"
     gene            2529..3497
                     /gene="tilS"
                     /locus_tag="PKV30_06345"
     CDS             2529..3497
                     /gene="tilS"
                     /locus_tag="PKV30_06345"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRL29871.1"
                     /translation="MDAFAAERPALPLAVALSGGADSTALLLACAARWPGRIEAWHVH
                     HGLQAAADDFEQHCVALCQRLGVPLHVRRVDARAAPGQSPEDAARRHRYEAFDALALA
                     SPARAAIKTVVIAQHADDQIETVLLALSRGAGLPGLAAMPARWVRGEVAYARPLLDVP
                     GAAIRAWLHRRGERWIEDPSNADERFTRNRIRARLLPAIEAVFPASRSTFARSARHAA
                     EAQLILNEVAAQDLAACGEPPCLKALQALSAPRQANALRHWLASAHAQSPSSAQLAEL
                     QSQIMAATTRGHRIDLKVGTGQVQRQADAIVWIAAPHASKPEPGAG"
     gene            3568..4836
                     /locus_tag="PKV30_06350"
     CDS             3568..4836
                     /locus_tag="PKV30_06350"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRL29872.1"
                     /translation="MALIVHKYGGTSMGSTERIAQVAKRVAKWARAGHQMVVVPSAMS
                     GETNRLLGLAKELAPGKPGADYYREQDMLAATGEQASSALLAIALQAEGQAAVSYAGW
                     QVPIKTSNAYTKARIDSIDDRRVRADLDGGKVVIITGFQGVDEGNNITTLGRGGSDTS
                     AVAVAAALKADEVLIYTDVDGVYTTDPRIVPEARRLLTVSFEEMLEMASLGSKVLQIR
                     SVEFAGKYKVPMRVLSSFTPWDIDLAEEARSGTLITFEEDDQMEQAVVSGIAFNRDEA
                     KISVLGVPDKPGIAFQILGTVADANIEVDVIIQNVSKDGRTDFSFTVHRNDYARTIDL
                     LKDKVVPALGAADVIGDPKICKVSIVGIGMRSHVGVAAKMFRVLSEEGINIMMISTSE
                     IKTSVVIDEKYMELAVRALHRAFDLDQPVA"
     gene            4853..4945
                     /locus_tag="PKV30_06355"
     tRNA            4853..4945
                     /locus_tag="PKV30_06355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4887..4889,aa:Ser,seq:gct)
     gene            5261..6433
                     /locus_tag="PKV30_06360"
     CDS             5261..6433
                     /locus_tag="PKV30_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRL29873.1"
                     /translation="MCVLIAASAWWLLVQVDRATGSLGLVYAVPPSLTHGAAMVLGFM
                     PLFFAGFLFTAGPNWLRMPHPEARPLLPPLGLQAAGWLLWLAGAHLNMALALVGLAAA
                     WIGLTWSVVLFWRLVLGSDVADRVHAKTVGVGGVVGVLCLAGVGLALAAGQPALALVL
                     VRTALWGFIVVTFVAVAHRMIPFFTSSVLPMIEIWRPFWVLWLMLGIAAFEVLALWVD
                     WALPPPRWWTLLVMAVEIAVGAVLIWLAVVWGLVQSLKIRLLAMLHVGFVWFGAALIY
                     SALSQGLWLATGQPVLGLGALHALSMGFLGSIMVAMVTRVSCGHSGRALVADDLVWGL
                     FWALQGAVLVRLIAAVQHAPAWLTTLAAALWAAVVIAWALRYARWYGQPRTDGKPG"
     gene            6399..7049
                     /locus_tag="PKV30_06365"
     CDS             6399..7049
                     /locus_tag="PKV30_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRL29874.1"
                     /translation="MDNRAPTANPADRPLAPLGALRPAEVAELAQALIQTRQSTLPKR
                     LAGPGPDAAQTQTILAAAASAPDHHELLPWRFVVVPEAARAALADAFAAALLQRDAAA
                     TPQQQAQAREKAFRAPLLLLAVVRLRDDDAEIEPHERIVSAGCALQNVLLMAHALGFG
                     GALTSGKALASAPLRRLFDLHDDEQALCFVNIGTVAEPKRARARPQAAQYVSVLQP"
     gene            7130..>7838
                     /gene="queC"
                     /locus_tag="PKV30_06370"
     CDS             7130..>7838
                     /gene="queC"
                     /locus_tag="PKV30_06370"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRL29875.1"
                     /translation="MHTRALVLFSGGQDSTTCLADALTRFEHVETIGFDYGQRHHVEL
                     AQRTVVLREMRSRFLDWAARLGDDHLIDVGVLGQISETSLTRELAFEMQASGLPNTFV
                     PGRNLVFLTLAGALAYRRGLDTLVTGVCETDYSGYPDCRDDTMKAMQLALSLGMDRRL
                     LIDTPLMWRDKAQTWQLAHALGQQADAQGGGDALVDLIVEHTHTCYAGDRTQCHAWGY
                     GCGACPACELRAAGWARW"
BASE COUNT         1267 a         2601 c         2651 g         1319 t
ORIGIN      
        1 cgcgccgccg ccaagcaagc gaagaatttc gccgaagccg accgcatccg cgccgagctt
       61 ctggcagccg gcatcgtgct gaaggattcg cccacgggca ccacctggga ggcggcgcag
      121 tgacgcagtt tcaagtgttt ttggcctctg gcgcttgcag gtaaagcggt ggcagctaca
      181 aaaaaagcag caattcaact gtccacgccc gcctactggg cggacgcctg cacgcacctg
      241 gccaagcgcg accgtgtgat gaagcgcctc atcccgcgct ttggcgatgc ctgcctgcag
      301 tcgcgcggcg atgcgtttgt cacgctctcg cgctctatcg tcgggcagca gatttccgtc
      361 aaggccgcgc agtcggtgtg gaaccgattc gaggccttgc ccaaacgcct gacgcccgcg
      421 cacgtgctga agctcaaggt ggacgacatg cgcgccgccg gcctgtcggc gcgcaaggtc
      481 gaatacctgg tcgatctggc gctgcacttc gactcgggcc gcatccatgt gaagcaatgg
      541 gacggcatgg acgacgaggc catcattgcc gagctggtgg ccattcgcgg catcggccgc
      601 tggacggccg agatgttcct catgttccat ctgctgcgcc ccaacgtgct gccgctcgac
      661 gacgtgggcc tgatcaacgg catcagcctg aactacttca gcggcgagcc ggtcagccgc
      721 agcgaggcgc gcgaggtggc cgaagcctgg gcgccgtggc gcagcgtggc cacttggtac
      781 atgtggcgct cgctggaacc gctgccggtg gcttattgag ggctggccac atccggtaac
      841 atcaactggt tgtcgttttc aaggagccca tgttggccaa acgaaccttt cttgacttcg
      901 agcagcccat cgcggagctt gagaacaaga tcgaagaact gcgctacgtg cagaacgaga
      961 gcgccgtcga tatcagcgaa gaaatcgacc agctcgacaa gaagagcctg cgcctcacca
     1021 aggacatcta tgccgatctg tcgccctggc agatcacgaa aatcgcgcgc caccccgagc
     1081 ggccgtacac gctcgactac gtgcgcgagt gcttcaccga ttttgtcgag atgcacggcg
     1141 atcgccactt tgccgacgac cagagcatcg tcggcggtct ggcgcgcttc aacggcaacg
     1201 cctgcatggt gattggccac cagaagggcc gcgacacgaa agagcgcacc ctgcgcaact
     1261 tcggcatggt gcggcccgag ggttatcgca aggcgctgcg gctgatgaag acggccgaga
     1321 agttcaagct gccggtgttc acctttgtcg acacgccggg cgccttcccg ggcatcgacg
     1381 ccgaggagcg cggccagtcc gaagccattg gccgcaacat ctacgagatg gcgcaacttg
     1441 aggtgcccat catcaccacc atcattggcg aaggcggctc gggcggcgcg ctggcgattg
     1501 cggtggccga ccaggtgctg atgctgcagt actcgatcta ctcggtgatc agccccgaag
     1561 gctgcgcgtc gattttgtgg aagaccagcg acaaggcgca ggacgcggcc gaggcgctgg
     1621 gcatcaccgc gcaccggctg aaggcgctgg gtctggtgga caagatcgtc agcgagccgg
     1681 tcggcggtgc gcaccgcgat catcggcaga tggcggcttt cctgaagcgc gctctgggcg
     1741 aggcgtggcg gcaggtgagc gacctgaagc cgaaggaact gctcgaccgc cgctatgagc
     1801 ggctgatgag ctacggccgc ttcaacgata ccaaggccga gcgctgaaga ggcgccgcgc
     1861 catcacatga caagggccgc gcgatgcggc ccttggtgtt ggtggcggtt tcatggctga
     1921 aaccacaaac catccgaccc cgttcgtcct gagcctgtcg aaggacttgg tgcaaaagac
     1981 cacggcttcg acaagctcag cccgaacggg tgtggcgttg tcgggctatt gatccgtcct
     2041 gccatgcaaa ctatcgtttt ggtagctgct cgcgcttgat agacaagcgc tggcggccca
     2101 aaacttgtaa aaaactcaag ccaccggccg cgtgcgcgcc gccaccttgc tgcgcagcgc
     2161 cgcgtgcaga tcgggcggca gaaacttgaa cgcgacctgc agcagcacgt aggcgatcag
     2221 gccatcgtcc agccagccga ggatggggat gaagtccgac accagatcga tcgggctcac
     2281 cacatacagc accgccagca ccaccgccag cttggccgac ttgggcgtgc gcggatcgcg
     2341 cagcacggcc caggccagca gcagttcctt gcggaacatt gcaaacagtt ttcccagttt
     2401 ccacatggcg cgatcctttt cgatcaaaga gggcacaggc cgtataacgt gggggcgccc
     2461 cgcccgatga caatgccgcc gtgaccgatt ctgccgcccc caccgatttc gcgctgctgg
     2521 acgccgccat ggatgcgttt gccgccgagc ggccagcgct gccgctggcg gtggccttga
     2581 gcggcggcgc tgattcaaca gcgctgctgc tggcctgcgc ggcgcgctgg ccagggcgca
     2641 tcgaagcctg gcacgtgcac cacgggctgc aagcggcagc cgacgacttt gagcagcatt
     2701 gcgtggcgct gtgtcagcgc ctgggcgtgc cgctgcatgt gcgccgcgtc gatgcgcgcg
     2761 ccgcgcccgg ccagagcccg gaagacgccg cgcgccgcca ccgctatgag gcttttgatg
     2821 ccctggcgct cgccagtcca gcgcgagcag ctatcaaaac cgtagtcatc gcacagcacg
     2881 ccgacgacca gatcgaaacc gtgctgctgg cgctttcacg cggcgccggc ctgcccgggc
     2941 tggcggccat gccggcgcgg tgggtgcgcg gcgaagtggc gtatgcgcgc cccttgctcg
     3001 acgtgcccgg cgccgccatt cgcgcctggc tgcatcggcg cggcgaacgc tggatcgaag
     3061 acccgagcaa cgcggacgag cgcttcaccc gcaaccgcat tcgtgcgcgc ctgctgccgg
     3121 ccatcgaggc ggtgttcccc gccagccgca gcacgtttgc ccgcagcgcc cggcacgcgg
     3181 ccgaggcgca actgatcctg aacgaggtcg cggcgcagga cttggccgct tgcggtgagc
     3241 cgccatgcct gaaggcgctg caggcgttgt cggcgccgcg ccaggcgaat gcgctgcgcc
     3301 actggctggc cagcgcgcac gcccagtcgc ccagcagcgc gcaactggcc gagctgcaat
     3361 cgcaaatcat ggccgccacc acgcgcggtc atcgcattga cctgaaggtg ggcaccgggc
     3421 aggtgcaacg ccaggcggac gccatcgtgt ggatcgccgc gccgcacgcc tcaaagccag
     3481 agcccggcgc cggctaaaat gcgcggtttg ccgctggcgc cccaccgggc tgttcggcgc
     3541 accgctttct cagcaagatt tcatgccatg gcattgatcg ttcacaaata cggcggcacc
     3601 tcgatgggtt cgaccgagcg catcgcccag gtcgccaagc gcgtggccaa atgggcgcgc
     3661 gccggccacc agatggtggt ggtgcccagc gccatgagcg gcgaaaccaa ccgcttgctc
     3721 ggcctggcca aggaactggc gcccggcaag cccggcgccg actattaccg cgagcaggac
     3781 atgctggccg ccactggcga gcaggcctcg tcggcgctgc tggccatcgc gctgcaggcc
     3841 gagggccagg ctgccgtcag ctacgccggc tggcaggtgc cgatcaagac cagcaacgcc
     3901 tacaccaagg cccgcatcga cagcatcgac gacaggcgcg tgcgcgccga cctcgatggc
     3961 ggcaaggtgg tcatcatcac cggctttcaa ggcgtggacg aaggcaacaa catcaccacg
     4021 ctggggcgcg gcggctccga tacgtcggcg gtggcggtgg cggcggcgct caaggccgac
     4081 gaggtgctga tctacaccga cgtcgatggc gtctacacca ccgacccgcg catcgtgccc
     4141 gaggcgcgcc ggctgctcac cgtgagcttc gaggaaatgc tggagatggc cagcctgggc
     4201 tccaaggtgc tgcaaatccg ctcggtggaa tttgcgggca agtacaaggt gcccatgcgc
     4261 gtgctctcca gcttcacgcc gtgggacatt gacctggccg aagaagcccg ctcgggcacg
     4321 ctgatcactt tcgaggaaga cgaccaaatg gaacaagccg ttgtttccgg cattgccttc
     4381 aaccgcgacg aagcgaagat ttccgtgctg ggcgtgcccg acaagccggg catcgcgttt
     4441 cagattctgg gcacggtggc cgacgccaac atcgaagtag acgtcattat ccagaacgtc
     4501 agcaaggatg ggcgcaccga tttctcgttc accgttcacc gcaacgacta cgcccgcacc
     4561 atcgacctgc tgaaagacaa ggtggtgccc gcgctgggcg cggctgatgt catcggcgac
     4621 ccgaagatct gcaaggtgag catcgtcggc atcggcatgc gcagccacgt gggtgtggcg
     4681 gccaagatgt tccgcgtgct gagcgaggag ggcatcaaca tcatgatgat ctccaccagc
     4741 gagatcaaga cctcggtcgt gatcgacgaa aaatacatgg aactcgccgt gcgcgccctg
     4801 caccgcgcct tcgatctgga ccagcccgtg gcataatcgc cgtcttcgcg ctggaaacgt
     4861 gaccgagtgg ccgaaggtgc tcccctgcta agggagtatg gggtgtagag cctcatcgag
     4921 ggttcgaatc cctccgtttc cgccagcgat tgaacatcaa cgggccttta cggcccgttt
     4981 ctttttttct gtgtttgagt cgaaggccaa gacagcgcca cagacccttg atcggcaagg
     5041 tcttgaaccc gatcaatgag gcgcggaccc cgatgcgggt acaacgcagg ccatgacagc
     5101 gccccctcca gcgccggcga gcacgccgcc gcgcagcata ccgatcggtt cagccgcgcc
     5161 tgcggcaccc aacccttcag tcaaacctcc ttctgttacg ggcccgtggc aatgggccaa
     5221 cctgttgcgg gcgccgcacc ggctggcgtt tttcctggcg atgtgcgtgt tgatcgccgc
     5281 cagcgcgtgg tggctgctgg tgcaggtcga tcgggccacg ggctcgctgg ggctggtgta
     5341 cgcggtgccg ccatcgctca cgcacggggc ggcgatggtg ctgggcttca tgccgctgtt
     5401 ttttgccggc ttcctgttca ccgccgggcc gaactggctg cgcatgccgc accccgaggc
     5461 gcggccgctg ctgccgccgc tcggcctgca ggccgctggc tggcttctgt ggctggccgg
     5521 cgcgcatctg aacatggcgt tggccctggt gggcctggcg gcggcgtgga tcggcttgac
     5581 gtggtcggtc gtgctgttct ggcgcctggt gctgggcagc gacgtggcgg accgcgtgca
     5641 cgccaagacc gtgggcgtgg gcggtgtcgt gggcgtgctg tgcctggccg gcgtggggct
     5701 tgcgctggcg gcgggtcaac ccgccctcgc gctggtgctg gtgcgcacgg cactgtgggg
     5761 cttcatcgtc gtcaccttcg tggcggtggc gcaccgcatg attccgttct tcacctccag
     5821 cgtgctgccg atgatcgaga tctggcggcc gttctgggtg ctgtggctga tgctgggcat
     5881 cgccgctttc gaggtgctgg cgctgtgggt cgattgggcc ctgccgccgc cgcgctggtg
     5941 gacgctgctg gtgatggcgg ttgagatcgc cgtcggcgcc gtgctgatct ggctggccgt
     6001 ggtgtgggga ctggtgcaaa gcctgaagat ccgcctgctg gcgatgctgc acgtcggctt
     6061 tgtgtggttt ggcgccgcgc tcatctacag cgcgctgtca caggggctgt ggctggccac
     6121 cgggcagccg gtgctggggc tgggcgcgct gcacgcgctg tcgatggggt ttctgggctc
     6181 gatcatggtc gccatggtca cgcgcgtgtc gtgcgggcac agcgggcgcg cgctggtggc
     6241 cgacgatctg gtgtggggcc tgttctgggc gctgcagggc gcggtgctgg tgcggctgat
     6301 cgccgccgtg cagcacgcgc cggcctggct gaccacgctg gcggccgcgt tgtgggcggc
     6361 ggtcgtcatc gcctgggcgc tgcgttacgc tcgctggtat ggacaaccgc gcaccgacgg
     6421 caaacccggc tgaccggcct ctcgcaccgc ttggcgcgct gcgccctgcc gaggtggccg
     6481 aactggcgca ggcgctcatc cagacgcgcc agagcacgct gcccaagcgc ttggccgggc
     6541 ctggcccgga cgcggcgcag acgcagacca ttctggccgc cgccgccagc gcccccgatc
     6601 accacgagct gctgccctgg cgcttcgtcg tggtgcccga agccgcgcgc gccgcgctgg
     6661 ccgacgcttt tgccgccgcc ctgctgcagc gcgacgccgc cgccacgccc cagcagcagg
     6721 cgcaggcgcg cgagaaggcg tttcgcgcgc cgctgttgct gctggccgtg gtgcgcctgc
     6781 gcgatgacga tgccgaaatc gagccgcacg agcgcatcgt ctcggccggc tgcgcgctgc
     6841 agaacgtgtt gctgatggcg cacgcgctcg gctttggcgg cgcgctgacc agcggcaagg
     6901 cgctggcctc ggcgccgctg cgccgcctgt tcgatctgca tgacgatgag caggcgctgt
     6961 gcttcgtcaa catcggcacc gtcgccgagc ccaagcgtgc gcgtgcgcgg ccgcaggcgg
     7021 cgcagtatgt gtcggtgctg cagccatgat taatgcataa aacatagctg ctcgcgcttt
     7081 tctctcaagc gctggcggcc gatttgattc agattcttcg agcctcacca tgcacacccg
     7141 cgcgctcgtg ctgttctccg gcggacagga ttccaccacc tgcctggccg atgcgctgac
     7201 gcgtttcgag catgtcgaga ccatcggctt cgactacggc cagcgccatc acgtcgagct
     7261 ggcgcagcgc accgtcgtgc tgcgcgagat gcgctcgcgc ttcctcgact gggcggcgcg
     7321 gctgggtgac gatcacctga tcgacgtcgg cgtgctgggc cagatcagcg agacctcgct
     7381 cacgcgcgag ctggccttcg agatgcaggc cagcggcctg cccaacacct tcgtgcccgg
     7441 gcgcaacctg gtcttcctga cgctggccgg agcgctggcc taccggcgcg ggctggacac
     7501 cctcgtcacc ggcgtgtgcg agaccgacta ttccggctac cccgactgcc gcgacgacac
     7561 catgaaggcg atgcagctcg cgctcagtct gggcatggac cggcgcctgc tgatcgacac
     7621 gccgctgatg tggcgcgaca aggcgcagac ctggcaactg gcgcatgcgc tgggccagca
     7681 ggccgatgcg caaggcggcg gcgatgcgct ggtcgatttg atcgtcgagc acacgcacac
     7741 ctgctacgcc ggcgaccgca cgcagtgcca cgcgtggggc tacggctgcg gcgcttgccc
     7801 ggcgtgcgaa ctgcgcgccg ccggctgggc gcgctggc
//
LOCUS       DAOMVO010000097         7824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2423_length_7824_cov_12.269790, whole genome shotgun sequence.
ACCESSION   DAOMVO010000097 DAOMVO010000000
VERSION     DAOMVO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2423_length_7824_cov_12.269790"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="PKV30_06375"
     CDS             complement(<1..2212)
                     /locus_tag="PKV30_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HRL29876.1"
                     /translation="MDLVYLLLLPFAGSLLAALMPTHARTAAAGWAALVSLAALVWVA
                     LLFPTLQTGQVLHEEYRWIPSAGINFSVRMDGFAWMFAMLVTGIGLLVALYARYYMSK
                     NDPVPRFFSFFLAFMGAMLGVVLSGNVVQLVFFWELTSLFSFMLIGYWYHRKDARRGA
                     RMALTVTGAGGLAMLAGMLMLGHIAGSYQLDVILGAGERIRAHPLYTPMLVLVLLGAF
                     TKSAQFPFHFWLPRAMAAPTPVSAYLHSATMVKAGVFLLARLWPALSGTEQWFWLVGG
                     AGVVTLVLGAYFAMFQNDLKGLLAYSTISHLGLITLLLGLNRDLAAVAAVFHIMNHAT
                     FKASLFMAAGIIDHETGTRDMRRLSGLWKAMPITGTLAFVASAAMAGVPLMNGFLSKE
                     MFFAETVDLSSTPLLDYGLPVAATLAGIFAVVYSLRFSVGVFLGPPARQLPLEPHEPV
                     RWMRVPIEILVLLCIVVGIFPQWSIGPALDVAARPVVGGAMPPFSLAIWHGFNKPLVM
                     SLIALGGGIALYLLLRRGVVAGRFKHAPLLGLNGQRLFESTLYGLDRASRWALAVLST
                     YRLQPQMLLMVLAAIVFASVALWAGGISWGDRPRVPGNLEFALLWVLGCVAALAAANQ
                     AKFHRLAALILLGAVGLAVSLTFLWFSAPDLALTQLTVEVVTTVLFLLGLRWLPKRRP
                     EEAEHLGLRVRARRARDFVVSLVAGGGMAVLAYAMMTRPAPQSISPFFIDRALPE"
     gene            2471..3133
                     /locus_tag="PKV30_06380"
     CDS             2471..3133
                     /locus_tag="PKV30_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL29877.1"
                     /translation="MRILLVEDDTMIGEVVHDALRAEHYAVDWVKDGAMAQTALATAP
                     YDLLLLDLGLPRQDGLTVLRALRAQKQRLPVLIATARDAVADRVAGLDAGADDYIVKP
                     YDLDELLARIRALLRRADGRAEPAYEWGEVRIVPATREVTLKGEPVPLSAREWAVLEP
                     LLARPGRVLSRQQLEEKLYSWRDDISSNAVEVYIHGVRKKLGAGLIENQRGLGYRVPP
                     GP"
     assembly_gap    3161..3260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3423..4760
                     /locus_tag="PKV30_06385"
     CDS             3423..4760
                     /locus_tag="PKV30_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL29878.1"
                     /translation="MKHEPKPAPSLRARLTGALLLAVLAFAALQAAVTYRTARAETEA
                     LFDAQMQRIALSLSGSLGAGALSDDAPAADTPAAREMIIQIWRADGVMLYRSPQGRLL
                     PPQTVIGFSDTVAGGEPYRIYALRTATQVVQVAQQTEARGRMAGQLALRAVLPVALLA
                     PVLMLIVWWVVGRAIGPIERVRRQVAARRPDDLAPLPTAGLPAEVRPLVGEMNGLLTR
                     LSAAWDALTHFSADAAHELRSPLAALRLQAQSLQRAPDDATRAIATERLLAGIDRATR
                     LVEQLLALARQEGAGEGAELVSLDLTALARNALADAEPEAARHAIALTLDAPTAHVVL
                     RADEAALAVLLRNLLGNALRHTSPGGQVRVGVREEASVIDLTVEDSGPGIAPDERARV
                     LDRFYRVPGTPGHGSGLGLAIVRAIAERHGAALTLDASPTLGGLRVMLRWPAV"
     gene            complement(4735..5550)
                     /locus_tag="PKV30_06390"
     CDS             complement(4735..5550)
                     /locus_tag="PKV30_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL29879.1"
                     /translation="MADLNERDSALRPEQPSIAIDDAFMRFRLPGSRASLNTGHIPSL
                     WISHGDALRVEAGPWSVTAQAVYVAPHCLHVVDGRGQLFQGVSLRGFRWPADVEPRPV
                     MALSPRALNLFVDHLQRQDGSIAHELAALLGFVPLRFSPAMSLLAERMVGDLMHRLSQ
                     TEASILTGMERTAMLKQFRRESGMSFRTFKTSAGVQAALRLMAVGRSVSEAAMASGFA
                     DLAHFSRHCRAATGYSPRQGLQYLDRALAMARAGQKRLAAADMPLITRQASAA"
     gene            5664..6245
                     /locus_tag="PKV30_06395"
     CDS             5664..6245
                     /locus_tag="PKV30_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29880.1"
                     /translation="MHKPDSQQSARLRVIGGLMLAVALTACGGSDDDDVPAPAPTPAP
                     SPSPSPSPSPSPSPSPSPANPDLASCPNFFPGFTSDTAYLQCMTGTYTGTLVSGAACK
                     LTFKGPGKGFSYDSAGISYSAPDAAFGNSIYGKSPDPVGQLEVIVNYDIVPNVSTVNY
                     KLHFSYLFGQLKTKTITVQQGNQGATSSCRIEL"
     gene            6340..>6533
                     /locus_tag="PKV30_06400"
     CDS             6340..>6533
                     /locus_tag="PKV30_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRL29881.1"
                     /translation="MNSLQLKSKKLVAALLAAGVLGGAGVVAVEHAHTPARAQAVTAQ
                     VAPAANTAAPTVAMPNFSAIT"
     assembly_gap    6534..6633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6634..7753
                     /locus_tag="PKV30_06405"
     CDS             <6634..7753
                     /locus_tag="PKV30_06405"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRL29882.1"
                     /translation="RGQGSGFIVSPDGLILTNAHVVRGADEVTVKLTDRREFKAKVLG
                     QDDKTDVAVLKIDATGLPTLPLGNTRTLAVGEWVLAIGSPFGFENSVTAGVVSAKGRS
                     LPDEGMVPFIQTDVAVNPGNSGGPLINASGQVVGINSQIYSRSGGYQGVSFAIPIELA
                     LHVKDQIVAHGKVEHARLGVSVQEVNQAFADSFKLPKPEGALVSQVEPGSAADKAGLK
                     SGDVIVRVDGQPVVASGDLPARIGMAKPGDSVKLEVWRQGKAEQLTAQLGGAKGKTTK
                     TADAGPANQGKLGLALRPLQPQEKAEAKVNGGLVVEQASGAAARAGVQPGDVVVSVNG
                     QPVTDIEQVRGLVAKADKSVALLIQRDGQQLFVPVRIG"
BASE COUNT         1314 a         2713 c         2558 g         1039 t
ORIGIN      
        1 cctcgggcag ggcgcggtcg atgaagaagg gcgagatgct ctgcggcgcc ggccgggtca
       61 tcatggcgta ggccagcacc gccatgccgc cgccggcgac cagtgagacg acgaagtcgc
      121 gcgcccggcg cgcccgcacg cgcaggccca ggtgctcggc ctcttccggc cggcgcttgg
      181 gcagccagcg caggcccagc aggaacagca ccgtggtcac cacctccacc gtcagctgcg
      241 tcagcgccag gtcgggcgcc gagaaccaca ggaaagtgag gctcaccgcc aaccccaccg
      301 cgcccagcaa aatgagcgcc gccaggcggt ggaacttggc ctggttggcg gcagccagcg
      361 cggccacgca gcccagcacc cacagcagcg caaattcaag gttgcccggc acgcgcgggc
      421 ggtcgcccca gctgatgccg ccggcccaca gcgccacgct ggcaaaaaca atggccgcca
      481 gcaccatcag cagcatctgc ggctgcaggc gataggtcga cagtaccgcc agcgcccagc
      541 ggctggcgcg atcgagcccg tacagcgtgc tttcaaacag gcgctgcccg ttcaggccca
      601 gcagcggcgc gtgcttgaag cgccccgcca ccacgccgcg ccgcagcagc aaatacagcg
      661 cgatgccgcc gcccagcgcg atcaggctca tcaccagcgg cttgttgaag ccatgccaga
      721 tcgccaggct gaagggcggc atggccccgc ccaccactgg ccgcgccgcc acgtccagcg
      781 ccgggccgat ggaccattgc ggaaagatgc cgaccacgat gcacagcagc accagtatct
      841 cgatcggcac gcgcatccag cgcaccggct cgtgcggctc cagcggcaac tggcgcgccg
      901 gcggccccag aaaaacgccg accgaaaagc gcagcgagta caccaccgca aagatgccgg
      961 ctagcgtggc cgccaccggc aggccgtagt cgagcaaggg cgtgctgctc aagtccaccg
     1021 tttcggcgaa gaacatctct ttcgacaaaa agccgttcat cagcggcacg ccggccatgg
     1081 ccgcgctggc gacaaaggcc agcgtgccgg tgatgggcat cgccttccac aggcccgaca
     1141 ggcggcgcat gtcgcgcgtg ccggtttcgt ggtcgatgat gccggccgcc atgaacagcg
     1201 aggccttgaa ggtcgcgtgg ttcatgatgt ggaacaccgc cgccaccgcc gccaggtcgc
     1261 ggttcagccc cagcagcagg gtgatcaggc ccaggtggct gatggtcgaa taggccagca
     1321 gccctttcaa atcgttctgg aacatggcga agtacgcccc cagcaccagc gtgaccacgc
     1381 ccgccccgcc gaccagccag aaccactgtt cggtgcccga gagcgccggc cacagccgcg
     1441 ccagcagaaa cacgcccgcc ttgaccatgg tggccgagtg caggtaggcc gacaccggtg
     1501 tcggcgccgc catggcgcgc ggcagccaga agtggaaggg gaactgcgcg ctcttggtga
     1561 acgcgcccag cagcaccagc accagcatcg gcgtgtacag cgggtgcgcg cggatgcgct
     1621 cgcccgcgcc caggatcacg tccaactggt agctgccggc aatgtggccc agcatcagca
     1681 tgccggccag catggccagc ccgcccgcgc ccgtcacggt gagcgccatg cgcgcgccgc
     1741 gccgcgcgtc cttgcggtgg taccagtagc cgatcagcat gaagctgaac aggctggtca
     1801 gctcccagaa gaacaccagc tgcaccacgt tgcccgacag caccacgccc agcatggcgc
     1861 ccatgaaggc gaggaagaac gagaaaaagc gcggcaccgg gtcgttcttg ctcatgtagt
     1921 agcgcgcgta cagcgccacc agcaggccga tgccggtcac cagcatggcg aacatccagg
     1981 caaagccgtc catgcgcacc gagaagttga tgccggctga tggaatccag cggtattcct
     2041 cgtgcagcac ctggccggtc tgcagcgtgg ggaacagcag cgccacccac accagcgccg
     2101 ccaggctgac cagcgccgcc cagcctgcgg ccgccgtgcg cgcgtgcgtg ggcatcagcg
     2161 cggcgagcag gctgccggca aacggcagca gcagcaggta gacgaggtcc atcgaggcgg
     2221 tcattgtctt ttaggttgtc gcccaccggg tcaccgaccg ggacaaggca tgcaagaaag
     2281 gacgaggcgg gggtcgaaaa acaagacgcg ggcagcgccg ggcgctgtcg cggtgcccac
     2341 tataccaatc tgcaacaaaa accagcccgg tcggcgccaa tcaagcggca ccagctacca
     2401 gcgtgatagc aaaaaaggcc cacgcagcgg cgcctgacgc ccggcgcggc ccgcttggca
     2461 caatcgtgcc atgcgcatcc tgctggtgga agacgacacg atgatcggcg aggtggtgca
     2521 cgacgccctg cgcgccgagc actacgccgt cgactgggtg aaagacggcg ccatggccca
     2581 gaccgcgctg gccaccgcgc cctacgacct gctgctgctc gacctgggcc tgccgcgcca
     2641 ggacggcctg acggtgttgc gcgcgctgcg cgcccagaag cagcgcttgc cggtgctgat
     2701 cgccaccgcg cgcgacgccg tggccgaccg cgtggcgggg ctggacgccg gcgctgacga
     2761 ttacatcgtc aaaccctacg acctggacga gttgctggcc cgcatccgcg ccctgctgcg
     2821 ccgcgccgac ggccgcgccg agccggcgta cgaatggggc gaggtgcgca tcgtgcccgc
     2881 cacgcgcgag gtcaccttga aaggcgagcc ggtcccgctg tcggcgcgcg agtgggccgt
     2941 gctggagcct ctgctggcgc gccccggccg cgtgctgtcg cgccagcagc tggaagaaaa
     3001 gctctactcg tggcgcgacg acatcagcag caacgcggtc gaggtctaca tccacggcgt
     3061 gcgcaagaag ctgggtgcgg gcttgattga gaaccagcgg gggctgggtt accgggtgcc
     3121 gccggggcca tgacgtcgct gcgctccatg acctcgctgc nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn tcgctgctcg gcggcttcaa tgaagaacga gcggcgcagt
     3301 gggccggctt tggtcatggc ggcgcagccg ccgtcatcga agcgcagcga ggtcaattcg
     3361 tcattgctac ttaaagaata gctgctcgcg cttgacagac aagcgctgga ggccaatttc
     3421 tcttgaaaca tgaaccaaaa cccgccccct cgctgcgcgc ccgcctgacc ggcgcgctgc
     3481 tgctggccgt gctggcgttt gcggcgctgc aggcggccgt cacctaccgc accgcgcgcg
     3541 ccgagaccga ggcgctgttc gacgcgcaaa tgcagcgcat cgcgctgtcg ctctcgggca
     3601 gcctgggcgc cggcgccttg agcgacgacg cgcccgccgc cgacacgccc gcggcgcgcg
     3661 agatgatcat ccagatctgg cgcgccgacg gcgtgatgct ctaccgcagc ccacagggcc
     3721 gcctgctgcc gccgcagacg gtgatcggtt tttccgacac cgtggccggc ggcgagccgt
     3781 accgcatcta cgccctgcgc accgccacgc aggtggtgca ggtggcgcag caaaccgaag
     3841 cgcgcgggcg catggccggc caactggcgc tgcgcgcggt gctgccggtg gcgctgctgg
     3901 cgccggtact gatgctgatc gtttggtggg tcgttggccg cgccatcggc cccattgaac
     3961 gcgtgcgccg ccaggtcgcc gcgcgccggc ccgacgacct ggcgccgctg cccacggccg
     4021 gcctgccggc cgaggtgcgc ccgctggtgg gcgagatgaa cggcctgctc acgcgcctgt
     4081 cagccgcgtg ggacgccctg acccacttct ccgccgacgc cgcgcacgag ctgcgctcgc
     4141 ccctcgccgc gctgcgcctg caggcgcaaa gcctgcagcg cgcgcccgac gacgccacgc
     4201 gcgccatcgc caccgagcgc ctgctggccg gcatcgaccg cgccacccgg ctggtcgagc
     4261 aactgctggc gctggcgcgt caggaagggg caggcgaggg cgccgagctc gtgtcgctgg
     4321 acctgaccgc gctggcgcgc aacgcgctcg ccgacgccga gcctgaagcc gcgcgccatg
     4381 ccatcgcctt gacactggac gcgccgacag cgcacgtcgt gctgcgcgcc gacgaagccg
     4441 cgctggccgt gctgctgcgc aacttgctgg gcaacgcgct gcgccacacg tcgccgggcg
     4501 gccaggtgcg cgtcggcgtg cgcgaagagg ccagcgtgat cgacctgacg gtggaagaca
     4561 gcggccccgg catcgcgccc gacgagcgcg cccgcgtact cgaccgcttc taccgtgtgc
     4621 ccggcacgcc cggccacggc agcggcctgg gcctggccat cgtgcgcgcg attgccgagc
     4681 ggcatggcgc ggcgctaacg ctggatgcct cgcccacgct gggcgggctg cgcgtcatgc
     4741 tgcgctggcc tgccgtgtga tcagaggcat gtctgctgct gccagccgct tctgcccggc
     4801 ccgcgccatg gccaaggcgc gatcgaggta ctgcaagcct tgccgtggcg aatagccggt
     4861 ggcggcacgg caatggcgcg agaaatgcgc cagatcggca aaaccgcttg ccatggccgc
     4921 ctctgacacc gagcgcccga cggccatcag gcgcagcgcg gcctgaacgc cggccgacgt
     4981 cttgaacgtc cgaaagctca tgcccgattc gcgccggaac tgcttgagca tggcggtgcg
     5041 ctccatgccg gtcaggatgc tggcctcggt ctggctcagg cggtgcatca ggtcgccgac
     5101 catgcgttcg gcgagcagcg acatggcggg tgaaaagcgc agcggcacga agccgagcag
     5161 ggcggccagt tcgtgcgcga tcgagccatc ctggcgctgc aggtggtcga caaacaaatt
     5221 cagggcccgc ggcgacaggg ccatcactgg ccgtggctcg acgtccgccg gccagcgaaa
     5281 gccgcgcaaa ctcacaccct gaaacaactg cccccgcccg tccacgacat gaaggcagtg
     5341 cggtgcaacg tagaccgcct gcgcggtgac gctccacggg ccagcctcga cgcgcagcgc
     5401 atcgccatgc gatatccaga ggctcgggat gtgcccggta ttcaggctgg cgcgactgcc
     5461 cggcagacga aaccgcatga aggcatcgtc gatggcgatg cttggctgct ccggccgcag
     5521 cgcgctatca cgttcattca agtcggccat gcagcggcag tttatcgacg cctgagcgcc
     5581 acggatgcac gttcgttcaa gtcacgcgca tcgatctcgg acagcatgcg gccttgtctt
     5641 ttcacgtttt tttggaggaa accatgcaca aacctgacag tcaacagtcg gcgcgcctgc
     5701 gcgtcattgg cggcctgatg cttgcggtcg cgctgaccgc gtgcggcggt agcgatgacg
     5761 acgacgttcc cgcgcccgcc ccgacgccgg cgcccagccc gtccccatca cccagtccat
     5821 cgccgtcgcc gtcgcccagc ccttcgccgg ccaaccccga cctggcgtcg tgccccaatt
     5881 tcttccctgg cttcacgtcc gacacggcgt acctgcaatg catgaccggc acctacaccg
     5941 gcaccctcgt cagcggcgcg gcctgcaagc tcacgttcaa aggcccgggc aagggcttca
     6001 gctacgactc ggccggcatc agctactcgg cgcccgacgc ggcctttggc aattcgatct
     6061 acggcaaatc gcccgatccg gtcggccagc tcgaggtcat cgtcaactac gacattgttc
     6121 ccaacgtcag caccgtcaac tacaagctgc acttcagcta cctgttcggg cagctcaaga
     6181 ccaagaccat caccgtgcag caaggcaacc agggcgccac gtccagctgc cgcatcgagc
     6241 tgtagggcgc atcgcgggtc taagcccccg ctaagtgttc accgccacat tcctgccatc
     6301 ccgccgcaat tcttggcggc ttaacgaaag gaatgtgaga tgaactcttt gcagctcaag
     6361 tccaagaagc tggtggccgc cttgctggcc gctggcgtgc tcggcggcgc gggcgtggtg
     6421 gcggtggagc acgcgcacac cccggcgcgc gcgcaagccg tgaccgccca ggtggcgccc
     6481 gccgccaaca cggcagcgcc caccgtggcc atgcccaact tcagcgccat cacnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgcggc caaggctcgg gcttcatcgt
     6661 cagccccgac ggcctgatcc tgaccaacgc ccacgtggtg cgcggcgccg atgaagtcac
     6721 cgtcaagctg accgaccggc gcgagttcaa ggccaaggtg ctgggccagg acgacaagac
     6781 cgacgtcgcc gtgctcaaga tcgacgccac cggcctgccc acgctgccgc tcggcaacac
     6841 gcgcacgctt gccgtgggcg aatgggtgct ggccatcggc tcgccgttcg gttttgaaaa
     6901 cagcgtgacc gccggcgtgg tgagcgccaa gggtcgctcg ctgccggacg agggcatggt
     6961 gcctttcatc cagaccgacg tggcggtgaa ccccggcaat tcgggcggcc cgctgatcaa
     7021 cgccagcggg caggtggtgg gcattaactc gcagatctac agccgctcgg gcggctacca
     7081 gggcgtgtcg tttgccatcc cgatcgagct ggcgctgcac gtcaaggacc agatcgtggc
     7141 gcacggcaag gtcgagcacg cgcggctggg tgtgtcggtg caggaagtga accaggcgtt
     7201 tgccgattcc ttcaagctgc cgaaacctga aggcgcgctg gtgtcgcagg tcgagcccgg
     7261 cagcgcggcc gacaaggcgg ggctgaagtc gggcgacgtg atcgttcgcg tcgatgggca
     7321 gccggtggtg gcgtcgggcg acttgccggc gcgcattggc atggccaagc cgggcgacag
     7381 cgtcaagctg gaagtctggc gccagggcaa ggccgagcag ctgaccgctc aactcggtgg
     7441 cgccaagggc aagaccacca agacggccga cgccggcccg gcgaaccaag gcaagctggg
     7501 cctggcgctg cgcccgctgc agccgcagga aaaagccgaa gccaaggtca acggcggcct
     7561 ggtcgtcgag caggccagcg gcgccgccgc gcgtgccggc gtgcagccgg gcgacgtggt
     7621 ggtgtcggtc aacggccagc cggtgacaga catcgagcag gtgcgcggcc tggtcgccaa
     7681 ggccgacaag tcggtggcgc tgctgattca gcgcgatggg caacagctct tcgtcccggt
     7741 gcgcatcggc tgacggcgcg catcgcgcca cagcccggcc cgccgctcca cgggccgccc
     7801 cgcaccgttt gcatgccgcc gttt
//
LOCUS       DAOMVO010000098         7819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2426_length_7819_cov_11.615018, whole genome shotgun sequence.
ACCESSION   DAOMVO010000098 DAOMVO010000000
VERSION     DAOMVO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_7819_cov_11.615018"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKV30_06410"
     CDS             <1..209
                     /locus_tag="PKV30_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HRL29883.1"
                     /translation="TADMVKPGAVVIDVGMNRDDAGKLCGDVDFAGVKEVAGWITPVP
                     GGVGPMTRAMLLVNTIEAAEQASA"
     gene            209..2356
                     /locus_tag="PKV30_06415"
     CDS             209..2356
                     /locus_tag="PKV30_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL29884.1"
                     /translation="MRRARPTALLLSLCVAAPGALAQDAAPEEADDDGALAPVVVRGS
                     RAGADRWQSPASMDVVNADDLKAGRPQISLSESLARVPGLVVRDRQNFAQDTQLSVRG
                     FGSRASFGVRGLKLYVDGIPASAPDGQGQTSHFPLSAAERIEVVRGPFAALYGASSGG
                     VLMLTTEDGRWPGEWRAGFTAGSDGLWRLSAQALGRTAPPDQLGWSYALSASGFSTDG
                     ARPQSAAAQGTANLKLTRQSGADRLVLLFNHHALRADDPLGLSRAEFDTHPRQTTPNA
                     LAYDTRKTVRQTQLGAAWEHRLNAEQRLELMGWLGQRGVWQYQAIPPAAQAAPGSAGG
                     VIDLDRAYGGAHARWRLDRPMGDATLGLVAGLTWSRQREQRAGYENFIGPHLGVLGRL
                     RRDETNLADAAEPYVQAEWRTPGWTLAGGLRHSHVRFTSRDHYIAPGNGDDSGRTRYS
                     GISPVIGGRVQLAESLQAFASVGGGFETPTLNEAAYSPGAQAGLNRGLSASRQQSAEI
                     GLRGRHGWGGWTATLFDVRTQDEIVPAQNLGGRTTFQNAGRTHRHGLELSADAEFGPV
                     TLTGAATFIEARFRDGYSKCAAPPCANPADVVPAGRRIPGIARQQLYAQLDWRLAWAQ
                     GAVLGLEARHTGRVPVNDANSDFAGAATVLALSLRHDITRGAWRLQPFLRVDNLTNRR
                     YAGSVIVNEGNGRFFEPAAGRSIFVGLEVRARR"
     gene            2607..4637
                     /locus_tag="PKV30_06420"
     CDS             2607..4637
                     /locus_tag="PKV30_06420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRL29885.1"
                     /translation="MANPLLDFTDLPLFDQVRPEHVGPAIDQLLAESEAALTAAVAPD
                     LPADWTTLSRTLDVATEKLGRAWGVVGHLNAVMDTPELRAAYNAALPKVTEFWTNLGS
                     DERLYAKYKKIDPATLNAEQRQAHKNALRGFVLGGAELQGAAKERYATIQEEQAALGQ
                     KFSENALDATDSYAYYATEAELAGVPPDVQAAARAAAEADGKGGYKLTLKLPSYLPVM
                     QFAHDGALRQRLYRAYVTRASDQAEGDAKKFDNAPLIQGILALRKEEAQLLGYANFGE
                     VSVVAKMAESPRQVIDFLRQLAAKARPYAERDMADLRAFAKDELKLADPQPWDWAYVG
                     EKLKEARYAFSEQELKQYFQAPKVLQGLFKIVETLFEVAIRRDSAPVWHPTVEFYRIE
                     RDGKLLGQFYLDPSARAGKRGGAWMDDVRARWLRPDTGQLQTPVAQLVCNFAEGVDGK
                     PPLLTHDDVITLFHEFGHGLHHMLTQVNEHDVSGISGVEWDAVELPSQFMENFCWEWD
                     VLRHMTAHVDTGAPLPRALFDKMIAAKNYQAGMQTVRQIEFALFDMLLHTEHDPKADF
                     QPLLDSVRQEVAVVQPPPFARTAQTFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEES
                     VTPDGLPSVETGRKYRREILEAGGSRPAMESFKAFRGREPSLDALLRHQGMA"
     gene            4697..5335
                     /locus_tag="PKV30_06425"
     CDS             4697..5335
                     /locus_tag="PKV30_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRL29886.1"
                     /translation="MSPAVLTAAVTIGLGALLLAGTAQAQVYRVVGPDGRVTYSDKPT
                     AQPARDAAPSSAGGSSNTALPYTLAQTAQRYPVTLYTSKECVPCTTGRNLLINRGIPF
                     SEKTVESNDDIAALQRLASAGSLPLLTIGGQQLKGYSDSEWTQYLDAAGYPKTSQLPS
                     SYRRPAAAPLVAARATPAPAASVAEAASAPAAPAQPSVAPPRSSTNPSGIRF"
     gene            complement(5418..6338)
                     /locus_tag="PKV30_06430"
     CDS             complement(5418..6338)
                     /locus_tag="PKV30_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HRL29887.1"
                     /translation="MRSAPWRIGLIGYGEVGKIFAAALVGQAGVASVGAWDLKFARPD
                     TAAAERDHAARAGVQAYDSAASLCAASDLVISAVTASNTLAVAEAAAPHMAPGALLLD
                     LNSASPGTKQQCAALIGGAGAHYVEAGVMTSVPPYGIRVPMLLGGARAAELAPVLAGW
                     GMDVRAVSEEIGVASAIKMCRSVMIKGLEALVIESYSTARAYGVESHVLPTLAETFPG
                     IDWEKQGAYFFSRVVQHGQRRAEEMRESARTVGEAGFEPFMARAIADKQQWVADQAQR
                     GVFAGLPGGAAWQDYADRLLAARHEGGETG"
     gene            complement(6413..7375)
                     /locus_tag="PKV30_06435"
     CDS             complement(6413..7375)
                     /locus_tag="PKV30_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29888.1"
                     /translation="MIHRRKLLLVASAAVIAPWALAAFPSKAVRIVVPNAPGGAADIT
                     ARAVGEHMASTLGQPVVIENKPGAGGVVAGQTVASAAPDGHTLLLISSGTAVSQALFK
                     SLPFDTLKAFTPVAPLATFDLVIVANEGGEFKMLADLIKFAKANPGKLNIGTPNIGTT
                     QHLAAELFKTAAGMDAQVVPFKGTPEVINAIRGGQIQAGLDILSPLLAQIQGKALRAL
                     AVTGAKRSRVLPDVPTAQEAGVKGLNAASWNGLAVPARTPADVVAQLNKAVGAALADA
                     GVRQKLEALNLDPHPGTPQDAAAMLSGDIQRWGDVIARAKIERQ"
     gene            7549..>7819
                     /locus_tag="PKV30_06440"
     CDS             7549..>7819
                     /locus_tag="PKV30_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRL29889.1"
                     /translation="MARASKDLSVEITGSKQEIAVVRLTRSAKRNALSDGLILALRDA
                     FQNLPATVRAAVLDGEGEHFCAGLDLSELKERDAGEGVHHSRMWHA"
BASE COUNT         1284 a         2845 c         2556 g         1134 t
ORIGIN      
        1 tgacggccga catggtcaag cctggcgcgg tcgtcatcga cgttggcatg aaccgcgacg
       61 acgctggcaa gctgtgcggc gacgtggact ttgccggcgt gaaagaagtc gccggctgga
      121 tcacccccgt gcccggcggc gtcggcccca tgacgcgcgc catgctgctg gtcaacacca
      181 tcgaagcggc ggaacaagcc agcgcctgat gcgccgcgcg cggccgaccg ccttgctgct
      241 gtcgctgtgc gtggcggcgc ctggggcgtt ggcgcaagac gccgcgccgg aagaagccga
      301 tgacgacggc gccctcgccc ccgtggtggt gcgcggcagc cgcgcgggcg cggatcgctg
      361 gcagtcgccg gcttcgatgg atgttgtcaa cgccgacgac ctgaaagccg gccgcccgca
      421 gatcagcctg tccgaatccc tcgcccgcgt gccgggcctg gtggttcgcg accggcagaa
      481 cttcgcccaa gacacccaac tgtcggtgcg cggctttggc agccgcgcca gttttggcgt
      541 gcgcggcctc aagctgtacg tggacggcat cccggccagc gcgcccgacg gccagggcca
      601 gaccagccac ttccccttga gcgcggccga gcgcatcgag gtggtgcgcg gcccgtttgc
      661 ggcgctctac ggcgccagca gcggcggcgt gctgatgctc accaccgaag acggccgctg
      721 gccgggcgaa tggcgcgccg gcttcaccgc cggcagcgac ggcctgtggc gcctgtcggc
      781 ccaggcgctg ggccgcaccg cgccgcccga tcagctcggc tggtcttacg ccctgtcggc
      841 cagcggcttc agcaccgacg gcgcgcgccc gcaaagcgct gccgcgcagg gcacggccaa
      901 cctcaagctc acgcgccaaa gcggcgccga ccggctggtg ctgctgttca accaccatgc
      961 gctgcgggcc gacgacccgc tcggcctgtc gcgcgccgaa ttcgacaccc acccgcgcca
     1021 gaccacgccc aacgcgctgg cctacgacac ccgcaagacc gtgcgccaga cgcagctggg
     1081 cgccgcgtgg gagcaccgcc tgaacgccga gcagcggctg gagctgatgg gctggctggg
     1141 ccagcgcggc gtgtggcaat accaggccat cccgcccgcc gcgcaggctg cgcccggctc
     1201 ggccggcggc gtgatcgacc tggaccgcgc ctacggcggc gcccacgcgc gctggcggct
     1261 cgaccgcccg atgggcgacg ccacgctggg cctggtcgcc ggcctgacgt ggagccgcca
     1321 gcgcgagcag cgcgccggct acgagaactt catcggcccg cacctgggcg tgctgggccg
     1381 cctgcggcgc gatgaaacca acctggccga cgcggccgaa ccctacgtgc aggccgaatg
     1441 gcgcacgccg ggctggacgc tggccggcgg cctgcgccac tcgcacgtgc gcttcacatc
     1501 gcgcgaccac tacatcgcac ccggcaacgg cgacgacagc gggcgcacgc gctacagcgg
     1561 catcagcccc gtcatcggcg ggcgtgtgca actggccgaa tcgctgcagg cgtttgccag
     1621 cgtgggcggc ggtttcgaga cacccacgct caacgaggcg gcctattccc ccggcgcaca
     1681 agccggcctg aaccgcggcc tgagcgccag ccgccaacaa agcgccgaaa tcggcctgcg
     1741 cgggcgccac ggctggggcg gctggaccgc cacgctgttc gacgtgcgca cccaagacga
     1801 gatcgtgccg gcgcagaacc tgggcggccg caccaccttc cagaacgcag ggcgcacgca
     1861 ccgccatggg ctggagctgt cggccgatgc cgaattcggc cccgtcacgc tgaccggggc
     1921 cgccaccttc atcgaggcgc ggtttcgcga tggctacagc aagtgcgccg ccccgccctg
     1981 cgccaacccg gccgacgtgg tgcccgccgg ccggcgcatt cccggcatcg cgcgccagca
     2041 gctgtatgcg cagctcgact ggcgcctggc ctgggcgcag ggcgccgtgc tgggcctgga
     2101 agcgcgccac accggccgcg tgcccgtcaa cgacgccaac agcgactttg ccggcgccgc
     2161 caccgtgctg gcgctcagcc tgcggcacga catcacacgc ggcgcctggc gcctgcagcc
     2221 gtttttgcgc gtcgacaacc tgaccaaccg ccgctacgcc ggctcggtga tcgtcaacga
     2281 gggcaatggc cgcttcttcg agccggcggc gggtcgttcg atctttgtcg ggctggaagt
     2341 gcgggcgcgg cggtgagcgc cgcctaatac cgaataccaa ataccggccg cgaaggacgc
     2401 aaagcattcg cgaaggacgc aaaagaacag ccaaaaggtt tttcttgaat cttcttgtct
     2461 tctgcgaaac ctctgcgccc ggctggtcgg acactgaaca atttcggaca aaataacgct
     2521 ccagcgcatg tccaaccagc gccagcagct atcatttcca tacttgcgtg cttgacattg
     2581 cacgccctgc ccccaactga cgcgccatgg ccaatcccct cctcgacttc accgacctcc
     2641 ccctgttcga tcaggttcgc cctgaacacg tgggccccgc cattgaccag ttgctggccg
     2701 aatccgaagc ggcgctgacc gccgccgtgg cgcccgatct gcccgccgac tggaccacgt
     2761 tgtcgcgcac gctcgacgtg gcgaccgaaa agctcggccg cgcctggggc gtggtcggcc
     2821 acctgaacgc cgtcatggac acgcccgagc tgcgcgccgc ctacaacgcc gcgctgccca
     2881 aggtgaccga gttctggacg aatctcggca gcgacgagcg gctgtacgcc aaatacaaaa
     2941 agatcgaccc cgccacgctg aacgccgagc agcgccaggc gcacaagaac gcgctgcgcg
     3001 gcttcgtgct gggcggcgcc gaattgcagg gcgcggccaa agagcgctac gccaccatcc
     3061 aggaagagca ggccgcgctg ggccagaagt tcagcgagaa cgcgctcgac gccaccgaca
     3121 gctacgccta ctacgccacc gaggccgagc tggccggtgt gccgcccgac gtgcaagccg
     3181 ccgcccgcgc cgccgctgaa gccgatggca agggcggcta caagctcacc ctcaagctgc
     3241 ccagctacct gccggtgatg cagttcgcgc acgatggcgc gctgcgccag cggctgtacc
     3301 gcgcctacgt cacccgcgcg tccgaccagg ccgagggcga cgctaaaaag ttcgacaacg
     3361 cgccgctgat ccagggcatc ctggcgctgc gcaaggaaga agcgcagttg ctcggctacg
     3421 ccaacttcgg cgaagtctcg gtggtggcca agatggccga atcgcccagg caagtgatcg
     3481 acttcctgcg ccagctggcc gccaaggcca ggccctacgc cgagcgcgac atggccgatc
     3541 tgcgcgcctt cgccaaggat gaactgaagc tggccgaccc gcaaccctgg gactgggcct
     3601 acgtcggcga aaagctcaag gaagcgcgct acgccttcag cgagcaagag ctgaagcagt
     3661 actttcaggc gcccaaggtg ctgcaagggc tgttcaagat cgtcgagacg ctgttcgagg
     3721 tcgccatccg ccgcgacagt gcgccggtgt ggcaccccac ggtcgagttc taccgcatcg
     3781 agcgtgatgg aaagctgctc ggccagttct acctcgaccc cagcgcgcgc gccggcaagc
     3841 gcggcggcgc ctggatggac gacgtgcgcg cccgctggct gcgccccgac accggccagc
     3901 tgcaaacgcc cgtggcgcaa ctggtatgca actttgccga aggcgtcgat ggcaaaccgc
     3961 cgctgctgac gcacgacgac gtcatcaccc tgttccacga attcggccac ggcctgcacc
     4021 acatgctgac ccaggtgaac gagcacgacg tgtcgggcat cagcggcgtt gagtgggacg
     4081 cggtggagct gcccagccag ttcatggaaa acttctgctg ggaatgggac gtgctcaggc
     4141 acatgacggc ccacgtcgac accggcgcgc cgctgccgcg cgcgctgttc gacaagatga
     4201 ttgccgccaa gaactaccag gccggcatgc agaccgtgcg ccagatcgag ttcgccctgt
     4261 tcgacatgct gctgcacacc gagcacgacc ccaaggccga cttccagccg ctgctcgaca
     4321 gcgtgcggca ggaagtggcc gtggtgcagc cgccgccctt cgcccgcacg gcgcagacct
     4381 tcagccacat ctttgccggc ggctacgcgg ccggctacta cagctacaag tgggccgagg
     4441 tgctgagcgc ggacgcgtat gccgcattcg aggaaagcgt cacgcccgac ggcttgccca
     4501 gcgtcgaaac cgggcgaaaa taccggcgcg agattctgga agctggcggc agccgcccgg
     4561 ccatggaatc gttcaaggcc ttccggggcc gcgagcccag cctggatgcg ctgctgcgcc
     4621 accagggcat ggcctgacaa cgccccgccg attccccgca ccgctggagt acttgcctgt
     4681 gccgcatcgc ccgcttttgt cccccgccgt tctcaccgcc gctgtcacca tcggtttggg
     4741 cgccctgctg ctggccggca ccgcccaggc gcaggtctac cgcgtggtgg ggcccgatgg
     4801 ccgcgtcacc tattccgaca aaccgacggc ccagccggcg cgcgacgcgg cgcccagcag
     4861 cgcgggcggc agcagcaaca ccgccctgcc ctacaccctg gcgcaaaccg cgcagcgcta
     4921 cccggtcacc ctgtacacca gcaaggaatg cgtgccctgc accacggggc gcaacctgct
     4981 gatcaaccgc ggcattccgt tcagcgagaa aaccgttgaa tcgaacgacg acatcgccgc
     5041 gctgcagcgc ctggccagcg ccggcagcct gccgctgttg accattggcg gccagcagct
     5101 caagggttat tcggacagcg aatggacgca gtacctggat gcggcgggct acccgaagac
     5161 ctcgcaactg cccagcagct accgccgccc cgcggcagcg cccttggtcg ccgcgcgcgc
     5221 taccccggcg cccgcggcat cggtggctga agccgcatcg gcaccggctg cgcccgcaca
     5281 gcccagcgtg gcgccgccgc gcagcagcac caacccgagc ggcatccggt tctgatttcg
     5341 cttctgattt gatagctgct cgcgcttgtc agacaagcgc tggcggccga aatggcttga
     5401 aaatcgagac tggctcgtca accggtctcg ccgccctcat ggcgcgccgc cagcagccga
     5461 tccgcatagt cctgccacgc ggcgccgccc ggcagacccg cgaacacccc gcgttgcgcc
     5521 tgatccgcca cccactgctg cttgtcggca atggcgcgcg ccatgaaggg ctcgaaaccc
     5581 gcctcgccca ccgtgcgcgc cgactcgcgc atctcctcgg cgcggcgctg gccgtgctgc
     5641 accacgcggc tgaagaagta cgcgccttgt ttctcccagt cgatgccggg aaaggtctcg
     5701 gccagtgtcg gcagcacgtg cgactcgaca ccgtaggcgc gcgcggtgct gtagctctca
     5761 atcaccagcg cctccagccc cttgatcatc acgctgcggc acatcttgat ggcgctggcg
     5821 acgccgatct cctcgctcac ggcgcgcacg tccatgcccc atccggccag caccggcgcc
     5881 agttcggccg cacgcgcgcc gcccagcagc atcggcacgc gaatgccgta gggcggcacc
     5941 gaagtcatca cgccggcctc gacgtagtgc gcgccggcgc caccgatcaa cgcagcgcat
     6001 tgctgcttgg tgccgggcga tgcggaattg agatcaagca gcagcgcgcc cggcgccatg
     6061 tgcggcgcgg ccgcttcggc caccgccagc gtgttcgatg ccgtcacggc cgagatcacc
     6121 aggtcgctgg ccgcgcacag cgacgccgcc gagtcgtacg cctgcacgcc cgcgcgcgcc
     6181 gcgtgatcgc gctcggcggc ggcggtgtca ggccgggcga atttcagatc ccacgcgccg
     6241 accgacgcca cgccggcctg gccgacgagc gcggcggcaa agatcttgcc gacctcgcca
     6301 tagcctatga ggccgatgcg ccagggtgct gatcgcatgt cgaatgctcc actattcata
     6361 gcttcttgcg cttgtcaatc aagcgctggc ggcacttttc gcttgaaaac actcattgcc
     6421 gctcgatctt ggcgcgcgca atcacgtcgc cccagcgctg gatgtcgccg ctcagcatgg
     6481 ccgccgcgtc ctgcggcgtg ccggggtgcg gatcgaggtt gagcgcttcg agcttttgcc
     6541 gcacgccggc gtcggccagg gcggcgccga cggccttgtt cagttgcgcc accacgtcgg
     6601 ccggcgtgcg tgcgggcacc gccaggccat tccacgacgc ggcgttcaga cccttgacgc
     6661 ccgcctcttg cgccgtcggc acgtcgggca gcacgcgcga gcgcttggcg ccggtcaccg
     6721 ccagcgcgcg cagcgccttg ccctggattt gcgccagcag cgggctcagg atgtcgagcc
     6781 cggcctggat ctggccgccg cggatggcgt tgatgacctc cggcgtgccc ttgaagggca
     6841 ccacctgcgc atccatgccg gccgcagtct tgaacagctc ggccgccagg tgctgcgtgg
     6901 tgccgatgtt gggcgtgccg atgttgagct tgcccggatt cgccttggcg aacttgatca
     6961 ggtcggccag cattttgaac tcgccccctt cattggcgac gatgacgaga tcgaacgtgg
     7021 ccagcggcgc gaccggggtg aaggctttga gcgtatcgaa cggcagcgac ttgaacagcg
     7081 cttggctcac cgccgtgccg ctggaaatca gcagcagcgt gtggccgtcg ggcgccgcgc
     7141 tggccaccgt ctgcccggcg acgacgccgc cggcgcccgg cttgttctcg atcaccaccg
     7201 gctggccgag cgtgctggcc atgtgctcgc cgactgcgcg cgccgtgatg tcggccgcac
     7261 cgccgggcgc attgggcacc acgatgcgca ccgccttgct cggaaaggcg gccagcgccc
     7321 acggcgcgat cacggcggcg ctggcgacca acaggagttt tcgtctgtga atcatgcgtc
     7381 tgtctccttg aaaacgggcc cgtgcccaac gacggcgcac gctctgctgg cattgtgacg
     7441 gccgcttggg gcttcggttg tatcaagggt aaaccccgat caaacggcgt ggtcggcggg
     7501 ttgaatttag ttattcacaa taatcataat cgagccaaag gagacaccat ggcccgagcc
     7561 agcaaggact tgtcggttga gatcaccggc agcaagcaag agatcgccgt cgtgcgcctg
     7621 acgcgcagcg ccaagcgcaa cgcgctgtcg gacggcctga tcctggcatt gcgcgacgca
     7681 ttccagaacc tgcccgccac cgtgcgcgcc gccgtgctgg atggtgaagg cgagcacttc
     7741 tgcgcaggcc tcgatttgag cgagctgaaa gagcgcgacg ccggcgaagg cgtgcaccac
     7801 tcgcgcatgt ggcatgcgg
//
LOCUS       DAOMVO010000099         7798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2437_length_7798_cov_14.054243, whole genome shotgun sequence.
ACCESSION   DAOMVO010000099 DAOMVO010000000
VERSION     DAOMVO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2437_length_7798_cov_14.054243"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /gene="rpoH"
                     /locus_tag="PKV30_06445"
     CDS             complement(<1..878)
                     /gene="rpoH"
                     /locus_tag="PKV30_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRL29890.1"
                     /translation="MTASVSAPAHAVAAANPWAVVPPLGNLDAYITAVNRLPMLTQEE
                     EQRFARELRDTGNIEAAGKLVLSHLRVVVSTSRQYLGYGLPHADLIQEGNVGLMKAVK
                     RFDPDQGVRLVSYALHWIKAEIHEYILKNWRLVKVATTKAQRKLFFNLRSMKQGFKAE
                     DAEQDITHRDVLSDAQIDVVARELNVKPEEVREMETRLSGGDILLDPAPSDDGEQAFG
                     PIAYLSDAHHEPTAMIESAQRDMLATEGIATALKSLDERSRRIVTERWLNVNDNGSGG
                     MTLHELAAEYGVSAER"
     gene            complement(1098..2096)
                     /locus_tag="PKV30_06450"
     CDS             complement(1098..2096)
                     /locus_tag="PKV30_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29891.1"
                     /translation="MKSIAARAIQASASALFLAFFGVSALAQAPGSSGYPTKPIRLVV
                     PFTPGGSTDVLARAIGQELTKAWGQSVVVENVPGAGGAIGADKVAKAAPDGYTLLMGH
                     IGTLAVNPSLYPRLPYNPVKDFAPVAWVARVPNVLVVHPSVAARNVKELVALAKSRPG
                     QLNYGSGGNGSAANLATEFLKLQTGTSLVHIPYRGTAPAVTDLMAGQIQLMFTGLPAL
                     VVPLKGGQLRALAVSSPQRLEALPGVPTVAESGYPGFEADQWYGVVAPAGTPRDVVAR
                     LNAQINEALAAPELKTRLAAEGAIATPTSPEAFGQLIASEITRWKPVIAGGRVKAD"
     gene            complement(2105..3016)
                     /locus_tag="PKV30_06455"
     CDS             complement(2105..3016)
                     /locus_tag="PKV30_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL29892.1"
                     /translation="MDLKQLSYFVRVAELGSFTRAAAALGVAQPALSRQIRLLEVELR
                     QNLLQRNGRGAVPTEAGALLLEHGRGILHQVARATDELSRLRGGLAGRVALGLPPSVA
                     RVLAVPLTRAIQAQLPGAQLSITEGLSAGMQEQLDNGRLDIAVLYNAQPLPGIELTPL
                     AHEELLLVQAAADAPAARGPIGLRELARLPLIIPSRPNAIRMQVEIEMTAAGCQPDVV
                     LEIDGVPAILDLVAGGAGCAVLSRNAVASAPRPAAFSVRRIKTARAPTLQIRLYTAVS
                     ALRPTTRTQQAALALVRASAAERFDAL"
     gene            3190..4581
                     /locus_tag="PKV30_06460"
     CDS             3190..4581
                     /locus_tag="PKV30_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HRL29893.1"
                     /translation="MSTIAPDPRGATDVQRFLNEHPFSRYQWVIFILCFLIVLMDGFD
                     TAAIGFIAPSLITEWGIGKSALGPVLSAALFGLAFGALTAGPLADRFGRKGVLTAAVV
                     VMGAASLISATSGNLTELTIWRFITGLGLGAAMPNAVTLINEYCPDSKRSFITNSMFC
                     GFPVGSAFGGFFAAWLIPNYGWRALLILGGVVPLLLVVLMLWLLPESVRYMVLRQQPA
                     ERIRKVLVRISHDARHASAFVLNEVRVGAAAATDAARKTGLALILSREFLLGTLMMWL
                     AYFMGLVIVYGLVNWMPVLFKDAGIAPARAAFIAAMFQLGGVGAIFFGLLMDRFNPNR
                     IIGIGYFVTCIAVALIGQAVGGGEGLLVAAVALAGLIMNASQSSMQALAANFYPTAGR
                     ATGVSWMLGIGRFGGIAGSFLVAELARRHVPLPQVFVVVGIPGLIAAAALFIKDRFGR
                     PQEAVPVQFAGAH"
     gene            4622..5650
                     /locus_tag="PKV30_06465"
     CDS             4622..5650
                     /locus_tag="PKV30_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL29894.1"
                     /translation="MIIDCHGHYTTAPKALENWRNQQIAGIKDPAAKPRVADLKISDD
                     ELRESIETNQLRLMKERGSDLTIFSPRASFMAHHVGDFEVSRTWAAICNELCYRVSQL
                     FPDHFIPAAMLPQSPGVDPATCIPELEKCVKEYGNVGINLNPDPSGGHWTSPPLTDRH
                     WYPIYEKMVEYDIPAMIHVSTSCNACFHTTGAHYLNADTTAFMQCLQGDLFKDFPTLK
                     FVIPHGGGAVPYHWGRFRGLAQEMKKPLLQDHLLNNIFFDTCVYHQPGIDLLTKVIPV
                     DNILFASEMIGAVRGVDPETGHYYDDTKRYIEASTIVVGDDRHKVYEANARRVYPRLD
                     AALKAKGK"
     gene            5653..6339
                     /gene="ligK"
                     /locus_tag="PKV30_06470"
     CDS             5653..6339
                     /gene="ligK"
                     /locus_tag="PKV30_06470"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HRL29895.1"
                     /translation="MYELGVVYRNIQRTDAATADALAPFGSATVHEAMGRVGLLKPYM
                     RPIYSGSQVSGTAVTVLLQPGDNWMLHVAAEQIQPGDIVVAGVTSDCTDGYFGDLLAT
                     SFKARGARALVIDAGVRDVRMLQHEIQFPVWSKAISAKGCIKATLGSVNVPVVCAGQI
                     VHPGDVIVADDDGVVCVPRAIAAKTLQAATAREANEGAKRAEFASGTLGLDYYKMREP
                     LAKAGLRYID"
     gene            6479..7420
                     /locus_tag="PKV30_06475"
     CDS             6479..7420
                     /locus_tag="PKV30_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL29896.1"
                     /translation="MSKPTTGPFEKTPGWLDWHEGPSKPRFKLPPGSVDAHCHVFGPG
                     AEFPYAPERKYTPCDASKEQLFALRDHLGFARNVIVQATCHGADNRAMVDACIASNGK
                     ARGVATVKRDVSDAELQRLHAAGVRGVRFNFVKRLVDFTPKDELMDIATRIHQLGWHV
                     VIYFEAVDLPELWDFFTALPNTVVVDHMGRPDVSKPVYGPEFALFIKFMREHPNVWSK
                     VSCPERLSVTGPKALNGEQAAYRDVVPFARNIVEQFPDRVLWGTDWPHPNLKDHMPDD
                     GLLVDFIPHIAPTAELQRKLLVDNPMRLYWPEEKAAR"
     gene            7426..>7798
                     /locus_tag="PKV30_06480"
     CDS             7426..>7798
                     /locus_tag="PKV30_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRL29897.1"
                     /translation="MAARQAPQSAPMWPADWRVLHASFVTETAGSTIWRVRLVGAPGT
                     AVAKVLKPAADAADELRAAHYLRWRAREGAVRLRAEDGLMLLLEDGGVAPLESLPDAA
                     AMPVAADVLARLHQPRPAPAAL"
BASE COUNT         1267 a         2625 c         2594 g         1312 t
ORIGIN      
        1 atgcgctcgg cgctgacgcc gtattcggct gccagctcgt gcagcgtcat gccgccggag
       61 ccgttgtcgt tcacgttcag ccagcgctcg gtcacgatgc ggcggctgcg ctcatccagg
      121 cttttcagcg cggtggcgat gccttcggtg gccagcatgt cgcgctgggc cgattcgatc
      181 atcgccgtcg gctcgtggtg cgcgtccgac aggtaggcga tggggccaaa ggcctgctcg
      241 ccgtcgtcgc tgggcgccgg gtcgagcagg atgtcgccgc ccgacaggcg cgtttccatc
      301 tcgcgcactt cctcgggctt cacgttcagc tcgcgcgcca cgacgtcgat ctgcgcgtcg
      361 gacagcacat cgcggtgcgt gatgtcttgt tcggcgtcct cggccttgaa accctgcttc
      421 atggaccgca ggttgaagaa cagcttgcgc tgcgccttgg tggtcgccac cttgaccagg
      481 cgccagttct tgaggatgta ctcgtggatc tcggccttga tccagtgcag cgcgtagctg
      541 accaggcgca cgccctggtc gggatcgaag cgcttgacgg ccttcatcag gccgacgttg
      601 ccctcttgaa tcaggtcggc gtgcggcagg ccgtagccca ggtactggcg cgaggtggaa
      661 acgaccaccc gcaggtgcga cagcaccagc tttccggcgg cttcgatgtt gccggtgtcg
      721 cgcaactcgc gcgcaaagcg ctgctcttcc tcctgcgtca gcatcggcag gcggttgacg
      781 gccgtgatgt aggcgtcgag gttgcccaag ggcggtacga ctgcccacgg attggccgcg
      841 gccacggcat gggcaggggc ggaaaccgaa gcggtcatgg gtgaatgtcc tttcatattg
      901 ctcaagtatt ttagcactct cttggagtga gtgccaaagg caaagttcaa cccgacttca
      961 cactttagcg gcgctgcggc gcaggcggcg tcagacaagt tcgatgggcg gcgattttca
     1021 agcaaaaaag ggtgctggcg cttgcccaac aagcgcaagc agctatcata aagatagtgc
     1081 ttacgtgggg tagttgctca atccgccttg acccgcccgc cggcaatcac cggcttccag
     1141 cgggtgattt cgctggcgat cagctggcca aaggcctcgg gcgaggtggg cgtggcgatg
     1201 gcgccttcgg ccgccaggcg ggttttcagc tcgggcgcgg cgagcgcctc gttgatctgg
     1261 gcgttgagcc tggccaccac gtcgcgcggc gtgccggccg gggcgaccac gccgtaccac
     1321 tggtcggcct cgaagcccgg gtagccgctt tcggccaccg tcggcacgcc gggcagcgcc
     1381 tccagccgct gcgggctgga gaccgccagc gcgcgcagtt gcccgccctt gagcggtacc
     1441 accaacgccg gtaggccggt gaacatgagc tggatctgcc ccgccatcaa atcggtcacc
     1501 gccggcgccg tgccgcggta ggggatgtgc accagcgacg tgccggtctg cagcttgagg
     1561 aattcggtcg ccaggttggc cgcgctgccg ttgccgcccg agccgtagtt gagctggccg
     1621 ggccgggact tggccagcgc caccagttcc ttgacgttcc tggccgccac cgacgggtgc
     1681 accaccagca cgttgggcac gcgcgccacc caagcgacgg gggcaaagtc cttcaccggg
     1741 ttgtagggca gcctggggta caggctgggg ttgacggcca gcgtgccgat gtggcccatc
     1801 agcagcgtgt agccgtcggg cgcggccttg gcgaccttgt cggcaccgat cgcgccgcca
     1861 gccccgggca cgttctcgac caccacgctc tggccccagg ccttggtcaa ttcctgcccg
     1921 atggcgcgcg ccagcacgtc ggtcgagccg ccgggcgtaa agggcacgac caggcggatc
     1981 ggcttggtgg ggtagccgct ggagccgggc gcctgggcca gcgctgatac accaaaaaat
     2041 gcaagaaaaa gcgcactggc gcttgcctgg attgcgcgag cagctattga tttcatagtc
     2101 tctctcaaag ggcgtcaaag cgttctgccg cgctggcgcg caccaaggcc aacgccgcct
     2161 gctgcgtgcg cgtggtcggg cgcagcgcgc tcacggcagt gtagaggcga atctgcagcg
     2221 tcggcgcgcg cgctgtcttg atgcgccgca cgctgaacgc ggccgggcgc ggcgcgctgg
     2281 ccaccgcgtt gcgcgacagc acggcgcagc ccgcgccacc ggccaccagg tcgaggatgg
     2341 ccggcacgcc gtcgatctcc agcaccacat cgggctggca gccggcggcg gtcatctcga
     2401 tctcgacctg catgcgaatg gcgttgggcc ggctggggat gatgagtggc agccgcgcca
     2461 gctcgcgcag gccgatcggc ccgcgcgcgg cgggcgcgtc ggcggcggcc tgcaccagca
     2521 gcagctcttc gtgggccaat ggcgtcagct cgatgcccgg cagcggctgg gcgttgtaga
     2581 gcacggcgat gtcgagccgg ccgttgtcca gctgctcctg catgccggcc gacaggcctt
     2641 cggtgatgga cagctgcgca cccggcaact gggcctggat ggcgcgcgtg agcggcaccg
     2701 ccagcacgcg ggcgacgctg ggcggcaggc cgagcgccac gcgcccggcg agcccgccgc
     2761 gcaggcgcga cagctcgtcg gtggcgcgcg ccacctggtg caggatgccg cggccgtgct
     2821 ccagcagcag cgcgccggcc tcggtcggca ccgcgccgcg gccgttgcgc tgcaacaggt
     2881 tctggcgcaa ttccacctcc agcaggcgaa tctggcggct gagcgccggc tgcgccacgc
     2941 ccagcgcggc ggcggcgcgc gtgaagctgc ccagctcggc cacgcgcacg aagtagctca
     3001 gctgtttcag gtccatcggc ggctcccggt tagcgcatac gcgcatgacg ttgatcaagc
     3061 tatagcaagt ggttatagct gatagtgggc gcgggcgctt cactggcgcg gctgcgggtt
     3121 caacaatcgg taccgagaca aggggcgccg tgaatccagc cgagcgcccg acaacggaga
     3181 caaaccctca tgagcacaat tgccccggac ccgcgcggcg ccaccgacgt ccagcgcttc
     3241 ttgaacgaac acccgttctc gcgctaccag tgggtgatct tcatcctgtg ctttttgatc
     3301 gtgctgatgg acggctttga caccgccgcc atcggcttca tcgcgccctc gctcatcacc
     3361 gaatggggca tcgggaagtc ggcgctcggc ccggtcttga gcgcggcgct gttcggcctg
     3421 gcctttggcg cgctgaccgc cggcccgttg gccgaccgct ttggccgcaa gggcgtgctg
     3481 acggcggcgg tggtggtgat gggcgcggcc tcgctgattt cggccacgtc cggcaacctg
     3541 accgagctga ccatctggcg cttcatcacc ggcctggggc tgggcgcggc gatgccgaac
     3601 gccgtcacgc tgatcaacga atactgccct gacagcaagc gatccttcat caccaacagc
     3661 atgttctgcg gctttccggt cggctcggcc tttggcgggt tcttcgccgc ctggctgatt
     3721 cccaactacg gctggcgcgc gctgctgatc ctgggcggcg tggtgccgtt gttgctggtg
     3781 gtgctgatgc tctggctgct gcccgaatcg gtgcgctaca tggtgctgcg ccagcagccg
     3841 gccgaacgca tccgcaaggt gctggtgcgc atctcgcacg acgcccgcca cgccagcgcc
     3901 ttcgtgctga acgaagtgcg tgtgggcgcc gcggccgcca ccgacgcggc gcgcaagacc
     3961 ggcctggcgc tgattctgtc gcgcgaattt ctgctcggca cgttgatgat gtggctggcc
     4021 tacttcatgg gcctggtcat cgtctacggc ctggtcaact ggatgccggt gctgtttaag
     4081 gacgccggca tcgccccggc gcgcgccgcc ttcatcgcgg ccatgttcca actcggcggc
     4141 gtgggcgcca tcttcttcgg gctgctgatg gaccgcttca acccgaaccg catcattggc
     4201 atcggctact tcgtcacctg catcgcggtc gccctgatcg ggcaggcggt gggcggcggc
     4261 gaagggctgc tggtggcggc ggtggcgctg gccggcctca tcatgaacgc gtcgcaatcg
     4321 tccatgcagg cgctggcggc caacttctac ccgacggcgg gccgcgcgac tggcgtatcg
     4381 tggatgctgg gcatcggccg attcggcggc attgccggct ccttcctggt ggctgagctg
     4441 gcgcgccgac acgtgccgct gccccaggtg ttcgtggtgg tgggcattcc gggcctgatc
     4501 gccgccgcgg cgctgttcat caaggaccgc ttcggccgcc ctcaggaggc ggtgccggtc
     4561 cagtttgccg gcgcgcactg aaagcctggc gcaccgcagt caaaaagagg agcgttgacc
     4621 catgatcatc gattgccacg gccactacac cacggcgccc aaggcgctgg agaactggcg
     4681 caaccagcag atcgccggca tcaaggaccc ggccgccaag ccgcgcgtcg ccgacctgaa
     4741 gatcagcgac gacgaactgc gcgagtcgat cgagaccaac cagctcaggc tgatgaaaga
     4801 gcgcggctcg gatctcacca tcttcagccc gcgcgccagc ttcatggcgc accacgttgg
     4861 cgacttcgag gtcagcagga cctgggcggc gatctgcaac gagctttgct atcgggtgtc
     4921 gcagctgttc cccgatcact tcattcccgc cgccatgctg ccgcagtcgc ctggcgtcga
     4981 tccggccacc tgcatccccg agctggaaaa gtgcgtcaag gaatacggca acgtcggcat
     5041 caacctgaac cccgatccat cgggcggcca ctggaccagc ccgccgctga ccgatcggca
     5101 ctggtatccc atctacgaaa agatggtgga gtacgacatc cccgccatga tccacgtcag
     5161 caccagctgc aacgcgtgct ttcacaccac cggcgcgcat taccttaacg ccgacaccac
     5221 ggccttcatg cagtgtctgc agggcgatct gttcaaggat ttcccgacgc tcaagttcgt
     5281 catcccgcac ggcggcggcg cggtgccgta tcactggggg cgctttcgcg gcctggcgca
     5341 ggagatgaaa aagccgctgc tgcaagatca cctgctgaac aacatcttct tcgacacctg
     5401 cgtgtaccac cagccgggca tcgatttgct gaccaaggtg atcccggtgg acaacatcct
     5461 gtttgccagc gaaatgatcg gcgccgtgcg tggcgttgac ccggagacag gccattacta
     5521 cgacgacacc aagcgctaca tcgaggcctc gaccattgtc gttggcgacg atcgccacaa
     5581 ggtctacgaa gccaacgcgc gccgcgtgta cccgcgcctg gatgcggcac tgaaagccaa
     5641 ggggaaataa gcatgtacga attgggcgtc gtttaccgca acatccagcg caccgatgcg
     5701 gccaccgccg atgcattggc ccccttcggc agcgccaccg tgcacgaggc catgggccgc
     5761 gtcggcttgc tgaagcccta catgcggccg atttactcag gcagccaggt cagcggcacg
     5821 gcggtcaccg tgctgctgca gccgggcgac aactggatgc tgcacgtggc ggccgagcag
     5881 attcagccgg gcgacatcgt ggtcgccggc gtcacctctg attgcaccga cggctacttc
     5941 ggcgacctgc tggccaccag cttcaaggcg cgcggcgcga gggcattggt gatcgatgcg
     6001 ggcgtgcgcg acgtgcgcat gttgcagcac gagatccagt ttccggtctg gagcaaggcc
     6061 atcagcgcca agggctgcat caaggcgacg ctgggcagcg tgaacgtgcc cgtggtctgc
     6121 gcggggcaaa tcgtgcaccc gggcgacgtg atcgtggccg acgacgacgg cgtggtctgc
     6181 gtgccgcgcg ccatcgccgc caagacactg caagccgcca ccgcgcgcga agccaacgaa
     6241 ggcgccaagc gcgccgagtt cgcctcgggc acgctgggac tcgactatta caagatgcgc
     6301 gagccgctgg ccaaggcggg gttgcgctac atcgactgat tgaacacaaa cagccggacg
     6361 cagaggacgc aaaggattcg caaaggacgc aaaaaagaaa accaaaaatt ttggctgttc
     6421 cttttgcatc ctctgcgcaa cttctgcgtc cggtattcgg catctgattt caaacaccat
     6481 gagcaaaccc accaccggcc cgttcgagaa aacccccggc tggctcgact ggcacgaagg
     6541 cccgagcaag ccacgcttca agctgccgcc cggcagcgtt gacgcgcatt gccacgtgtt
     6601 cggccccggt gccgagttcc cctacgcgcc cgagcgcaag tacacgccgt gcgatgcgtc
     6661 gaaggaacag ctgttcgcgc tgcgcgatca cctcggcttt gcgcgcaacg tgatcgtgca
     6721 ggccacctgc cacggcgccg acaaccgcgc catggtcgat gcctgcatcg catcgaacgg
     6781 caaggcgcgc ggcgtggcga cggtgaagcg cgatgtgagc gacgccgagc tgcaacgcct
     6841 gcacgccgcc ggcgtgcgcg gcgtgcgctt caacttcgtc aagcggttgg tcgatttcac
     6901 gcccaaggac gagctgatgg acatcgccac gcgcatccat caactcggct ggcatgtggt
     6961 gatctacttt gaggcggtcg atctgcccga gctgtgggac tttttcaccg cgctgcccaa
     7021 cacggtggtg gtcgaccaca tgggccgccc cgacgtgagc aagccggtct atggccccga
     7081 gttcgcgctg ttcatcaagt tcatgcgcga acaccccaac gtgtggagca aggtgagctg
     7141 ccccgagcgc ctgtccgtca ccggccccaa ggcgttgaac ggcgagcagg ctgcctaccg
     7201 cgacgtggtg cccttcgcgc gcaacatcgt cgagcagttt cccgaccgcg tactgtgggg
     7261 caccgactgg ccgcacccca acctgaagga ccacatgccc gacgatggcc tgctggtcga
     7321 ctttatcccg cacatcgcgc ccacggccga gctgcagcgc aagctgttgg tcgacaaccc
     7381 gatgcgcctg tactggcctg aagaaaaggc cgcgcgttga tccccatggc cgcacgccaa
     7441 gccccgcaat cggcgcccat gtggcccgcc gactggcggg tgctgcacgc cagcttcgtc
     7501 accgagacgg cgggcagcac catctggcgc gtgcggctgg tcggcgcgcc tggcacggcg
     7561 gtggccaagg tgctcaagcc agcggccgac gccgccgacg agttgcgcgc cgcgcactac
     7621 ctgcgctggc gcgcgcgcga gggcgcggtg cgcctgcggg ccgaagacgg actgatgctg
     7681 ttgctggaag acggcggtgt ggccccgctg gaatcactgc ccgacgccgc tgcgatgccc
     7741 gtggccgccg acgtgctggc gcgtttgcac cagccgcgac cagcaccagc ggccctgc
//
LOCUS       DAOMVO010000100         7610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2530_length_7610_cov_16.475447, whole genome shotgun sequence.
ACCESSION   DAOMVO010000100 DAOMVO010000000
VERSION     DAOMVO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_7610_cov_16.475447"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PKV30_06485"
     CDS             complement(<1..829)
                     /locus_tag="PKV30_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HRL29898.1"
                     /translation="MKHYIGAGNPMRAQFDARAPYQAKHFAWNCDANGVGTVSLNRPE
                     RKNPLTFDSYGELRDLFNGLRYATDVKVVVVTGEGGNFCSGGDVHDIIGPLTKMTMPE
                     LLEFTRMTGDLVKAMRGCPQPVIGAIDGVCAGAGAMMALACDLRYGTDKTKTAFLFTR
                     VGLAGADMGACALLPRVIGQGRASELLFTGRAMTAQEGLAWGFFNALHDSGDLLVKAQ
                     AMAAELAAGPTFAHGMTKTMLSQEWAMTIEQAIEAEAQAQAICMQTQDFKRAYEAFAA
                     "
     gene            complement(826..1290)
                     /locus_tag="PKV30_06490"
     CDS             complement(826..1290)
                     /locus_tag="PKV30_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRL29899.1"
                     /translation="MDLEARAHAEHPDELRLWLRLLTCTQLIEKQVRTDLREQWGTTL
                     PRFDLMAQLEREPEGMRMNELSRRMMVTGGNITGITDQLEAEGLVERAEVEGDRRAYL
                     VRLTPKGRQQFNAMAQAHEAWIVDAFASLSARDIQTLHALLGKVKDHFQETA"
     gene            complement(1295..3736)
                     /locus_tag="PKV30_06495"
     CDS             complement(1295..3736)
                     /locus_tag="PKV30_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HRL29900.1"
                     /translation="MNIVCIGGGPAGLYFALLMKQHNPAHEITVVERNKPYDTFGWGV
                     VFSDATMDNMRQWDTVTAGQIEQAFNHWDDIELDFKGEVIRSGGHGFVGIGRKKLLNI
                     LQARCEQLGVKLVFETDVNSDAEYPGADLIIASDGINSKIRTLHQDVFQPELVVRPNR
                     YIWLGTNRLYDAFTFVFEKTEHGWFQAHIYKFDESTTTFIVECPEHVWLAHGLDKATQ
                     DESIAFCEKLFAGTLRGYPLMTNARHLRGSAWLNFQRVKCEQWWLKNEHGAHLVLMGD
                     AVHTAHFAIGSGTKLAIEDAIELTRLFIEMGDAPDTIGDVLTHYQATRSVEALKLQNA
                     AWNAMEWFEVCGERYCDTLEPPQFMYSMLTRSQRISHENLRLRDAAWLEGYEDWFART
                     RTAPVSSEPNRPPALVNKARAASVSGATVPPMLTPFTVRGLTLKNRIVVSPMAQYSAQ
                     DGVVGDWHLMHLGARAVGGAALVMVEMTSPTHRHGFDGRISPGCPALESDAQQAAFKR
                     IVDFVHASSDAAIGIQLGHSGPKGSTQLGWEAIDEPLAQDNWPLVAASAVPYGAQNQT
                     PQSLTRADMDALRDAFVAATRRADAAGFDWLELHCAHGYLLASFISPLTNQRTDEYGG
                     SLENRCRYPLEVFGAMRAAWPQDKPMSVRISAHDWAPGGTTPADALAMAKLFKAAGCD
                     VIDVSSGQTTRAAKPVYGRMYQTPFADRIRNEAGIATMAVGAISEADHANSIIAAGRA
                     DLCAVGRPHLADPAWTLHEAAKLQSQAIAWPRQYLSGRDQMYREQAKQKAALAPVSRA
                     PAASDLVANVAASTV"
     gene            3899..4243
                     /locus_tag="PKV30_06500"
     CDS             3899..4243
                     /locus_tag="PKV30_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HRL29901.1"
                     /translation="MLYKFKSPEAADVIMLKPSAEEILKIIGKSPGPTGIVTAAQAAS
                     AVAALKAEIAQREARGQGEAAPTNADDARAQAEKDQVAHQELVSLRQRAAPFIELLER
                     SAAAGKDVVWGV"
     gene            complement(4340..4738)
                     /locus_tag="PKV30_06505"
     CDS             complement(4340..4738)
                     /locus_tag="PKV30_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29902.1"
                     /translation="MTSPPLRIVDSITELRPHDAGCVAISGSHGGISSAQYALAARPL
                     IAVFNDAGVGKDRAGLAALPFLQAHGIAACAVRHDSARIGEARSTLDEGVIGHCNALA
                     EALGASPGLACSVLVGRLTAQAAAEKSNVK"
     gene            complement(4809..5798)
                     /locus_tag="PKV30_06510"
     CDS             complement(4809..5798)
                     /locus_tag="PKV30_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRL29903.1"
                     /translation="MTKLFKLTLAAGAAVLAVGAHAQAFPAAGKTITLIVPFAAGGPT
                     DKVARDLAEALRKPLGGTTIVVDNAAGAGSTIGTAKAARATPDGYTLLVTHVGMATTP
                     TLYRNPGYKYDEFEYLGLINEVPMVVVAKPQMAANTWAELNTWIGQNKGKVNLANAGL
                     GSASHLCGLMLQNALKSDMTTVPYKGTAPAMTDLIGGQVDIMCDQTTNTTSQVEGKKI
                     KAYAMSTAKRITTPAVYKDLPTLDEAGLKGFNVSIWHGLYAPKGTPAAVVKTLNDALK
                     VALKDPEFIKKEEGLGAVVVTDGRVNPAEHKKFVASEVAKWGAVIKAAGQYAD"
     gene            complement(5938..7188)
                     /locus_tag="PKV30_06515"
     CDS             complement(5938..7188)
                     /locus_tag="PKV30_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29904.1"
                     /translation="MIDYFDALETRTPAEREAALMAALPRQIAHAQAHSAAMGEILAG
                     FDAAAITSRAALAQLPVTRKSELLARQQASRAAGRDAFGGFSAIRYGAAMPRLYASPG
                     PIYEPEGTGRDYWRAARAMFAAGFRAGDLAHNAFSYHMTPGAFIMESGAHAVGCTVFP
                     AGTGQTEQQLQAIAELKPQAYLGTPSFLRILVEKAQEAGVDIRSLAKGLVGGEAFPPS
                     LRDWFTERGMAIYQSYATADVGLIAYETASREGLVLDEGVIVEIVRPGTGDPVGEGEV
                     GEIVVTVLGPDYPLIRFGTGDLSAVLPGACPTGRTNTRIKGWLGRADQTTKIRGMFVH
                     PGQVAEIVKRFPEVSRARLVVSGEMANDQMKLLVESAAAQGLSERVAEVVRDVTKLRG
                     DVEVVAPGSLPNDGKVIEDARSYK"
     gene            complement(7434..>7610)
                     /locus_tag="PKV30_06520"
     CDS             complement(7434..>7610)
                     /locus_tag="PKV30_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29905.1"
                     /translation="KYADYGYIMESGRVVMDGRASDLASNEDVKEFYLGVGGGERKSF
                     KDVKSYKRRKRWLA"
BASE COUNT         1165 a         2516 c         2556 g         1373 t
ORIGIN      
        1 tggccgcgaa cgcctcgtac gcgcgcttga agtcctgcgt ctgcatgcag atggcctgcg
       61 cttgcgcttc ggcctcgatg gcttgctcga tggtcatggc ccactcttgc gacagcatgg
      121 tcttggtcat gccatgcgca aacgtggggc cggcggccag ctcggcggcc atggcttgcg
      181 ctttgacgag caaatcgccg ctgtcgtgca gcgcgttgaa gaagccccag gccagtcctt
      241 cttgcgccgt catcgcgcgg ccggtgaaca gcagttcgct cgctcgcccc tgcccgatca
      301 cccgcggcag cagcgcgcag gcgcccatgt cggcgccggc caaaccgacg cgggtgaaca
      361 ggaaagccgt cttggtcttg tcggttccat agcgcaggtc gcaagccagc gccatcatcg
      421 cgcccgcacc ggcacagacg ccatcgatgg cgccaatgac gggctgcggg cagccgcgca
      481 tggctttcac cagatcgccc gtcatgcgtg tgaattccag cagctcgggc atcgtcattt
      541 tggtgagcgg gccaatgatg tcgtgcacgt cgccgcccga gcagaagttg ccgccctcgc
      601 ccgtcaccac gaccaccttc acgtcggtcg cgtagcgcag gccgttgaac aagtcgcgca
      661 gctcgccata gctgtcgaag gtgagcgggt tcttgcgctc gggtcggttg aggctgacgg
      721 tgccgacgcc gttcgcgtcg cagttccagg cgaagtgttt cgcctggtag ggcgcgcggg
      781 cgtcgaactg ggcgcgcatg gggttgcccg cgccgatgta gtgcttcatg ccgtctcctg
      841 aaaatggtct ttcaccttgc cgagcaaggc gtgcagggtt tgaatgtcgc gtgcgctcaa
      901 ggacgcgaag gcgtccacga tccatgcctc gtgcgcctgc gccatggcgt tgaattgctg
      961 gcggcccttg ggggtcagcc gcaccaggta ggcgcgccgg tcgccctcga cctcggcgcg
     1021 ctcgaccaga ccttcggcct ccagctggtc ggtgatgccg gtgatgttgc cgcccgtcac
     1081 catcatgcgg cgcgacagct cgttcatgcg catgccctcg ggctcgcgct cgagctgcgc
     1141 catcaggtcg aagcgcggca gcgtggtgcc ccactgctcg cgcagatcgg tgcgcacctg
     1201 cttttcaatc agctgcgtgc aggtgagcag gcgcagccac aggcgcagct catcggggtg
     1261 ttcggcgtgg gcgcgggctt ccagatccat cgcgtcacac cgtgctggcc gccacgttcg
     1321 ctaccaaatc agaagctgct ggcgctcgac tgacgggcgc cagcgccgct ttttgcttcg
     1381 cctgctcgcg gtacatctga tcgcgcccac tcaggtactg gcgcggccac gcaatggcct
     1441 ggctttgcag cttggcggct tcgtgcagcg tccacgccgg gtcggccagg tgcgggcggc
     1501 ccacggcgca caggtcggcg cggccggcag caatgatgct gttggcgtga tcggcctcgc
     1561 tgattgcccc gaccgccatg gtggcgatgc ccgcttcgtt gcggatgcgg tcggcaaacg
     1621 gcgtctggta catgcggccg tacaccggct tggcggcgcg cgtggtctgg ccggatgaga
     1681 cgtcgatcac gtcgcagccc gcggccttga acagcttggc catcgccagt gcatcggccg
     1741 gcgtggtgcc gccgggtgcc cagtcgtggg cggagatgcg cacgctcatc ggcttgtctt
     1801 gcggccaggc ggcgcgcatg gcgccgaaca cctccagcgg gtagcggcag cggttttcca
     1861 gcgagccgcc gtactcgtcc gtgcgctgat tggtcagcgg gctgatgaag gacgccagca
     1921 ggtagccgtg cgcgcagtgc agctccagcc agtcgaagcc ggcggcatcg gcacggcgtg
     1981 tggcggccac gaaggcatcg cgcagcgcgt ccatgtcggc acgcgtgagc gattgcggcg
     2041 tctggttctg cgcgccatac ggcacggcgc tggcggcgac cagcggccag ttgtcttggg
     2101 ccagcggttc gtcaatcgcc tcccagccga gttgcgtcga gcccttgggc ccactatgcc
     2161 cgagctggat gccgatggca gcgtcgctgc tcgcgtgcac gaaatcgacg atgcgcttga
     2221 aggccgcctg ctgcgcatcg ctctccagcg ccgggcagcc ggggctgatg cggccgtcaa
     2281 agccgtggcg gtgcgtgggc gaggtcatct cgaccatcac cagcgctgcg ccgccgacgg
     2341 cgcgtgcgcc caggtgcatc aagtgccagt cgccgaccac gccgtcttgc gcgctgtact
     2401 gcgccatcgg cgagacgacg atgcggttct tgagcgtcag cccacgcacc gtgaaagggg
     2461 taagcatggg cggcaccgtt gctcctgaaa cagaagctgc tcgcgcttta tttacaagcg
     2521 ctggaggccg atttggttca gaactaacgg gcgccgtccg cgtgcgcgca aaccaatctt
     2581 catagccttc cagccacgcc gcatcgcgca gacgcaggtt ctcgtggctg atgcgctggc
     2641 tgcgcgtgag catgctgtac atgaactgcg gcggctccag cgtgtcgcaa tagcgctcac
     2701 cgcagacctc gaaccattcc atcgcattcc aggcggcgtt ctgcagcttg agcgcctcga
     2761 cgctgcgcgt ggcctggtag tgcgtcagca catcgccaat ggtgtcgggc gcatcgccca
     2821 tttcgatgaa cagacgcgtc agctcgatcg cgtcctcgat cgccagcttg gtgcccgagc
     2881 cgatggcgaa gtgcgccgtg tgcaccgcat cgcccatcag cacaaggtgc gcgccgtgct
     2941 cgttcttcaa ccaccactgc tcgcacttca cgcgctgaaa gttcagccag gccgagccgc
     3001 gcaggtggcg cgcgttggtc atcaacgggt agccgcgcag cgtgccggca aacagctttt
     3061 cgcagaaggc aatcgattcg tcctgcgtcg ccttgtcgag cccgtgcgcc agccacacgt
     3121 gctcggggca ctcgacgatg aaggtggtgg tgctttcgtc gaacttgtag atgtgggcct
     3181 gaaaccagcc gtgctcggtc ttctcgaaca cgaaggtgaa ggcgtcgtac aggcggttgg
     3241 tgccgagcca gatgtagcgg ttggggcgca ccaccagctc gggctggaac acgtcctggt
     3301 gcagcgtgcg gatcttggag ttgatgccgt cgctggcgat gatgaggtcg gcgccggggt
     3361 attcggcgtc cgaattgaca tcggtctcga acaccagctt gacgcccagt tgctcgcagc
     3421 gcgcctgcag gatgttgagc agcttcttgc gcccgatgcc gacaaagccg tggccgccgg
     3481 agcggatcac ctcgcccttg aagtccagtt cgatgtcgtc ccagtggttg aaggcctgct
     3541 cgatctggcc cgcggtcacc gtgtcccact ggcgcatgtt gtccatggtg gcgtcggaga
     3601 acaccacgcc ccagccaaag gtgtcgtagg gcttgttgcg ctcgaccacg gtgatctcgt
     3661 gcgccgggtt gtgctgcttc atcagcagtg cgaagtacag gccagcgggg ccgccgccga
     3721 tgcagacgat gttcatgatc gaatagttta tatgtcaatt aaatggcgtc aagtggtttg
     3781 atcaaacgcg tcgcgctttc gtccgacgca ccgggcaggc cggcggcggc acaatggcag
     3841 cagtgcgcca tgcactgcgc cagcgcggct ggcgccctgc ctagaccgga gaaaacgaat
     3901 gctctacaaa ttcaaatcgc cagaagccgc cgacgtgatc atgctgaaac cctcggccga
     3961 ggagatcctg aaaatcattg gcaagtcgcc tgggcccacc ggcatcgtga cggccgcgca
     4021 ggctgcgtcc gcggtggcgg cgctgaaggc cgagatcgcc cagcgcgagg cgcgcggcca
     4081 gggcgaggcg gcgcccacca acgccgacga tgcgcgcgca caggccgaga aggaccaggt
     4141 cgcgcaccag gaactggtgt cgctgcgcca gcgggcagcg cccttcatcg agctgctgga
     4201 gcgcagcgca gcggcgggca aggatgtggt ctggggcgtg tgaaacggcg ccgatcaggc
     4261 ggctcggtcg gctgacggca ctgaacagaa cactatcgat aacatagctg ctcgcgctta
     4321 tccaccaagc gccagcggcc tatttgacgt tagatttctc tgcggcagct tgcgccgtca
     4381 gcctgcccac caacacactg cacgccagcc ctggcgatgc gcccagggct tcggccagcg
     4441 cgttgcagtg gccgatcacg ccctcatcca gcgtactgcg cgcctcgccg atgcgggcgc
     4501 tgtcgtggcg cacggcgcag gccgcgatgc cgtgggcttg aagaaacggc agcgcggcca
     4561 gacccgcccg atccttgccg acgccagcgt cgttgaagac cgcgatcaac ggccgcgctg
     4621 ccagcgcgta ctgcgccgag ctgatgccgc cgtgcgagcc gctgatggcg acgcagccgg
     4681 catcgtgcgg gcgcagctcg gtgatcgaat cgacgatgcg cagcggtggt gatgtcatgg
     4741 cttgagaaaa gcaaaaaagc cgcgcgagtg tggccaccgc gcggcttttt ttgttgccct
     4801 tcaggcgatc agtcggcgta ctgccccgcg gccttgatga cggcgcccca cttggcgact
     4861 tcggaagcca cgaacttctt gtgctcggcc gggttgacgc gcccgtcggt caccacgacc
     4921 gcgcccaggc cttcttcctt cttgatgaat tccgggtcct tcagcgcgac tttcagggcg
     4981 tcgttcagcg tcttgaccac ggcggccggc gtgcccttgg gcgcgtacag gccgtgccag
     5041 atcgagacgt tgaagccctt caggccggcc tcgtccagcg tcggcaagtc cttgtacacg
     5101 gccggcgtgg tgatgcgctt ggcggtgctc atggcgtagg ccttgatctt cttgccctcg
     5161 acctggctgg tggtgttggt ggtctggtcg cacatgatgt cgacctggcc gccgatcaga
     5221 tcggtcatgg cgggcgccgt tcccttgtac ggcacggtgg tcatgtcgga cttgagcgcg
     5281 ttctgcaaca tcagcccgca caggtgcgac gcgctgccga ggccggcgtt ggccaggttg
     5341 accttgccct tgttctggcc gatccaggtg ttcagctcgg cccaggtgtt ggcggccatc
     5401 tgcggcttgg cgaccaccac catcggcact tcgttgatca ggccgaggta ttcaaactcg
     5461 tcgtacttgt agccggggtt gcggtacagc gtgggcgtgg tggccatgcc gacgtgcgtg
     5521 accagcagcg tgtagccgtc cggcgtggcg cgcgcggcct tggcggtgcc gatggtgctg
     5581 ccggcgccgg ccgcgttgtc gaccacgatg gtggtgccac ccagcggctt gcgcagcgct
     5641 tcggccaagt cgcgcgccac cttgtcggtc gggccacccg cggcaaacgg cacgatcagg
     5701 gtgatggtct tgccggcggc gggaaaagcc tgggcgtgcg cgcccaccgc cagcacggcg
     5761 gcgccagcgg cgagggtcag cttgaacagc ttggtcatgt gaaggaggct ccttgtaaat
     5821 tgacaaccgg acgatggtac cgacagacaa cggcccccac catccgggcg tttaccgagc
     5881 gcgtcggggt ttgcccttgc ccgaacgcaa aactcgggta aggcgtgccg ttttggctta
     5941 cttgtagctg cgcgcgtcct caatcacctt gccgtcgttg ggcaggctgc cgggcgccac
     6001 cacttccaca tcgccacgca gcttggtcac atcgcgcacc acctccgcca cgcgttcgga
     6061 caaaccctgc gcggcggccg attcgaccag cagcttcatc tggtcgttgg ccatctcgcc
     6121 gctgaccacc aggcgcgcgc ggctcacttc gggaaagcgt ttgacgatct cggccacctg
     6181 gccggggtgc acgaacatgc cgcgaatttt ggtggtctgg tcggcgcggc cgagccagcc
     6241 cttgatgcgc gtgttggtgc ggccggtggg gcaggcgccg ggcagcacgg cggacagatc
     6301 gccggtacca aagcggataa gcgggtagtc ggggccgagc acggtgacca cgatctcgcc
     6361 cacttcgcct tcgcccaccg gatcaccggt gccggggcgc acgatttcga cgatcacgcc
     6421 ctcgtccagc accaggcctt cgcgcgacgc ggtttcatac gcgatcaggc cgacgtcggc
     6481 cgtggcgtag ctctggtaga tggccatgcc gcgctcggtg aaccagtcgc gcaacgaggg
     6541 cggaaacgcc tcgccgccga ccagcccctt ggccaggctg cggatgtcca cgccggcctc
     6601 ttgcgccttc tcgaccagga tgcgcagaaa gctgggcgtg cccaggtagg cctgcggctt
     6661 caactcggcg atcgcctgca gttgctgctc ggtctggccc gtaccggcgg ggaacacggt
     6721 gcagcccacc gcgtgcgcgc cgctttccat gatgaaggcg ccgggcgtca tgtggtagct
     6781 gaaggcgttg tgcgccaggt cgccggcgcg aaagccggcg gcgaacatgg cccgggccgc
     6841 gcgccagtag tcgcgccccg tgccctcggg ctcgtagatg gggcccgggc tggcgtacag
     6901 gcgcggcatg gcagcgccgt agcggatggc ggaaaaaccg ccgaacgcat cgcgcccggc
     6961 cgcgcgcgag gcctgctggc gcgccagcag ttcgctcttg cgcgtgacgg gcagctgggc
     7021 cagcgccgcg cgcgatgtga tcgcagcggc atcgaagccc gccagaatct cgcccatcgc
     7081 cgcgctgtgc gcctgcgcgt gcgcgatctg ccgcggcagc gcggccatca gcgccgcctc
     7141 acgctcggcc ggcgtgcgcg tttcgagtgc gtcgaagtag tcgatcattg catcatcctt
     7201 ggcttgcaat tactcttgat ttgatagctg cttgcgcttg atggacaagc gccaggggcc
     7261 gatttgattc acaggccgtg aacagaccgg ggtggccctc acccctgccc tctcccagag
     7321 ggagaggggg tttccttgca gaccgcctct tccagtgcac ccgcggacgg ctctgccgcc
     7381 cgctgggtgc gtccccctca gggggaaggc gcgtcagcgc ctcaggggga gcgtcaagca
     7441 agccagcgct tgcgccgctt gtagctcttg acatccttga agctcttgcg ttcgccgccc
     7501 ccgacgccca ggtagaactc cttcacgtcc tcgttgctcg ccagatcgct cgccctgccg
     7561 tccatcacca cgcggccgct ttccatgatg tagccgtagt cggcgtactt
//
LOCUS       DAOMVO010000101         7594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2542_length_7594_cov_15.037008, whole genome shotgun sequence.
ACCESSION   DAOMVO010000101 DAOMVO010000000
VERSION     DAOMVO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_7594_cov_15.037008"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="metG"
                     /locus_tag="PKV30_06525"
     CDS             complement(<1..1124)
                     /gene="metG"
                     /locus_tag="PKV30_06525"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRL29906.1"
                     /translation="MSAPRQIFVTTALPYANGNFHIGHIMEYIQADIWVRYQRMQGHA
                     VNFVGADDAHGAPIMIAAEKAGKTPEQFVADIQAGRKPYLDGFLISHDNWHSTHSPEN
                     TELAQAIYKDLKAGGLIESRTIEQFFDPEKGMFLPDRFIKGECPNCHAKDQYGDNCEV
                     CGAVYAPTELIEPYSALSGAKPELKTSEHFFFKLSDARCIDFLKQWTTSGAVQPEVLN
                     KITEWIAPGDDGQPKMGDWDISRDAPYFGIEIPEAPGKYFYVWLDAPIGYLASLKNLL
                     EKRGESYDAYMAQPDLEQYHFIGKDIITFHTLFWPAMLKFSGRKVPNKVFVHGFLTVN
                     NGEKMSKSRGTGLDPLKYLSLGMNPEWLRYYIAAKLSGRN"
     gene            1268..2362
                     /gene="apbC"
                     /locus_tag="PKV30_06530"
     CDS             1268..2362
                     /gene="apbC"
                     /locus_tag="PKV30_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HRL29907.1"
                     /translation="MSIQQPDVLQALSAVIDPNTGNDFVSTRQLKNLQVDANGDVAFD
                     VELGYPAKSQIPALRQALVAAARGVPGVGNVSANLATHITAHAVQRGVPLLPQVKNIV
                     AVASGKGGVGKSTTAANLALALAAEGARVGLLDADIYGPSMPIMMGVSGRPESLDGQN
                     MEPLENYGVQVMSIGFLVDPDEAMIWRGPMATQALDQLLRQTSWKELDYLIVDMPPGT
                     GDIQLSLSQRVPMTGAVIVTTPQDIALADAKKGVAMFEKVGVPILGLVENMAVHVCSN
                     CGHVEHIFGAEGGKRYAAQKGIDYLGALPLSLAIREQADSGRPSVIADPDGEAARSYK
                     AIARQVAIRIAAKAKDFSARFPAINISRDT"
     gene            2530..3267
                     /locus_tag="PKV30_06535"
     CDS             2530..3267
                     /locus_tag="PKV30_06535"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRL29908.1"
                     /translation="MNRFDLDWLSQWSGLADGRLAPLLGTVVALLALMLMWNLARRPR
                     DPRAVAQAYDASSDRFSQTAPINEEQVQLLRYLQQAFPDGAVLFRPRLARFLTVRKSS
                     HRLGAQQRLADAQVDFLVCADDGKPLFAFEVDAFRHPDDADIARNAAEKNRMLKSADI
                     RLIRLKGALPNWPPAEILRMRLLAAQRTPAAEGRPSGFSPSGFGPSSFAHTDFAHSRS
                     ANSDVMGLSRLMGMPATDDPWESVRKR"
     gene            complement(3362..4930)
                     /locus_tag="PKV30_06540"
     CDS             complement(3362..4930)
                     /locus_tag="PKV30_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HRL29909.1"
                     /translation="MTPEVAPPPERFNFAEHLLALNANRAGKPAFVDEQGTVTYGELA
                     ERTRRMASALKSLFLRREERVLVLMHDGCDWPVAFLGALYAGLVPVAVNTLLTADDYA
                     YMLEHSRAQAVLVSGALLPTLTAAMVKSDHEVQKVIVSQPQAPLHPSEVEFEAFLKAH
                     AQLDKPAATGADDPAFWLYSSGSTGRPKGTVHSHANPYWTCELYGKGVLDLRESDVCF
                     SAAKLFFAYGLGNALTFPMSVGATTLLMGERPTPEATFKRWTGQVGGLKPTVFYGAPT
                     GFAGMLAHPALPKREDVAMRLVSSAGEALPAEIGERFKAHFGVDIVDGIGSTEMLHIF
                     LSNLPHKVRYGTTGWPVPGYEVELRGDDGGPVPDGEPGDLYIAGPSAAMMYWGNRSKT
                     RDTFQGGWTKSGDKYVRNSDGSYTYGGRSDDMLKVSGIYVSPFEVEATLVQHPAVLEA
                     AVVGKPDTDGLTKTKAFVVLKAGAEASEDELKAFVKDRLAPYKYPRFIEFVNELPKTA
                     TGKIQRFKLREQAP"
     gene            5162..6088
                     /locus_tag="PKV30_06545"
     CDS             5162..6088
                     /locus_tag="PKV30_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL29910.1"
                     /translation="MSTELQERPPTDAAPAEPPRHPFLVALGERVRNLRSRRGMTRKA
                     VAQAAEVSERHLANLEYGTGNASILVLLQVAQALHCDLSELLGDVTTSSPEWLMIREL
                     LEHRSEPDLRRARQQLSELFHAPGAADRARTTRIAVIGLRGAGKSALGQRLAQDLGYA
                     FIELSREIEQFAGCSINEIHNLYGTNAYRRYERRALEEAIQIYPEVVIATPGGLVADA
                     ANFNLLLQHCYTIWLQADPGDHMGRVAAQGDMRPMAASAEAMEDLKRILAGRSAFYAK
                     ADLRFNTSDYDSEEAAFRALRQSVQEATGIKA"
     gene            6178..>7594
                     /gene="boxC"
                     /locus_tag="PKV30_06550"
     CDS             6178..>7594
                     /gene="boxC"
                     /locus_tag="PKV30_06550"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HRL29911.1"
                     /translation="MPAPQVEYRTDPSQYRHIKLSFDGATAQLAIDIDENAGLRPGYK
                     LKLNSYDLGVDIELHDALQRIRFEHPEVKTVVVTSAKDKVFCSGANIFMLGVSSHGWK
                     VNFCKFTNETRNGIEDSSQHSGLKFIAAVNGACAGGGYELALACDEIILVDDRNSSVS
                     LPEVPLLGVLPGTGGLTRVTDKRHVRHDLADIFCTSVEGVRGQRAVDWRLVDAVAKPQ
                     VFNDTVAKRASELAAGSHRGAGQGVALTPIERQIDGDTISYKNVSIDISRGKRNATFL
                     VKAPQGDQPKDIAGIEAAGVNWWPLAMARELDDAILHMRTNELDIGTWILKTEGDSAA
                     VLAADATMEAHREHWFVNETIGMLRRTFARLDVSSRSLFALIEPGSCFAGTLAELVFC
                     ADRAYMLDDENAASITLDSFNFGLLPMVNGQSRLARRFYEEAAPMEAVRAAAGKPLGP
                     KEAEKLGLITAAPDDIDWADEV"
BASE COUNT         1348 a         2441 c         2496 g         1309 t
ORIGIN      
        1 tcgttgcggc ccgacagctt ggcggcgatg tagtagcgca gccattcggg attcatgccc
       61 aggctcaaat acttgagcgg gtcgagcccg gtgccgcggc tcttgctcat cttttcgccg
      121 ttgttcacgg tgagaaagcc gtgcacgaag accttgttcg gcaccttgcg gcccgagaac
      181 ttgagcatgg ccggccagaa cagcgtgtgg aaggtgatga tgtccttgcc gatgaagtga
      241 tattgctcca gatcgggctg cgccatgtag gcgtcatagc tctcgccgcg cttttccagc
      301 aggtttttca ggctggccag gtagccgatg ggcgcgtcga gccagacata gaaatacttg
      361 cccggtgcct cgggaatctc gatgccgaag tagggcgcgt cgcggctgat gtcccagtcg
      421 cccatcttgg gctggccgtc gtcgccgggg gcgatccatt cggtgatctt gttcagcacc
      481 tcgggctgca cggcgccgct ggtggtccac tgcttcaaaa agtcgatgca gcgcgcatcg
      541 gacagcttga agaagaagtg ctcgctggtc ttcagctcag gcttggcgcc gctcagggcc
      601 gaatacggct cgatcagctc ggtcggcgca tagacggcgc cgcacacttc gcagttgtcg
      661 ccgtactggt ccttggcgtg gcaattcggg cactcgccct tgatgaagcg gtcgggcaaa
      721 aacatgccct tctcggggtc gaaaaactgc tcgatggtgc gcgactcgat caggccaccc
      781 gccttcaagt ccttgtagat ggcctgcgcc agttcggtgt tctcggggct gtgcgtgctg
      841 tgccagttgt catggctgat cagaaagccg tccagatacg gcttgcggcc ggcctggatg
      901 tcggccacga actgctcggg cgtcttgccg gctttttcag ccgcaatcat gatcggcgcg
      961 ccgtgcgcat cgtcggcgcc gacaaagttg accgcgtggc cctgcatgcg ctggtaccgc
     1021 acccagatgt cggcctggat gtattccatg atgtggccga tgtggaaatt gccgttggca
     1081 tacggcagcg cggtggtgac aaagatctgg cggggggcgg acatgcggcg cggctctttc
     1141 ggggagggaa cccggcattt taggcagtgg ccggtctatt catcggcggc gggtgaggcc
     1201 atggccgcgc acccggggcg ccccggcagc ccgcgactag actagcggct ccaccggaag
     1261 agagcacatg agcatccagc aaccagacgt cttgcaggcc ctgtcggccg tgatcgaccc
     1321 caacacgggc aacgattttg tctccaccag gcagttgaag aacctgcagg tcgatgccaa
     1381 cggcgacgtg gccttcgacg tcgagctggg ctacccggcc aagagccaga ttccggcgct
     1441 gcgccaggcg ctggtggcgg ccgcgcgcgg cgtgcccggc gttggcaacg tgtcggccaa
     1501 cctggcgacc cacatcaccg cccacgcggt gcagcgcggc gtgccgctgc tgccgcaggt
     1561 gaagaacatc gtggccgtgg cctcgggcaa gggcggcgtc ggcaaaagca ccaccgccgc
     1621 caacctggcg ctggcgctgg cggccgaagg cgcgcgcgtc ggcctgctgg acgccgacat
     1681 ctacggcccc agcatgccca tcatgatggg cgtcagcggg cggccggaaa gcctggacgg
     1741 ccagaacatg gagccgctgg aaaactacgg cgtgcaggtc atgtcgatcg gctttctggt
     1801 cgacccggac gaggccatga tctggcgcgg ccccatggcc acgcaggcgc tcgatcagct
     1861 gctgcggcaa accagctgga aagagctgga ttacctcatc gtcgacatgc cgcccggcac
     1921 cggcgacatc cagctctccc tctcgcagcg cgtgccgatg accggcgccg tcatcgtcac
     1981 cacaccgcag gacatcgccc tggccgacgc gaaaaaaggc gtggccatgt tcgagaaggt
     2041 cggcgtgccg atcctcgggc tggtcgagaa catggcggtg cacgtgtgca gcaactgcgg
     2101 ccacgtcgag cacatcttcg gcgccgaggg cggcaagcgc tacgcggccc aaaaaggcat
     2161 cgactacctg ggcgcgctgc cgctgtcgct ggcgatccgc gagcaggccg attcgggccg
     2221 cccgagcgtc atcgccgacc ccgacggcga ggccgcgcgc agctacaagg ccattgcgcg
     2281 ccaggtggcg atccgcattg ccgccaaggc caaggatttt tcggcgcgct ttccggccat
     2341 caacatctcg cgcgatacct gattttgctt ttgttttgat agctgctcgc gcttgtccag
     2401 aaagcgctgg cgggcgattt ttatcggacc gcgtgacggg tgattaggtc atttggcatc
     2461 accaaaaagg gtgatagcgc gggccgttca agtggcgtaa ggtgtacccc aaatcaggga
     2521 gtacaaacaa tgaaccgctt cgatctggac tggctgtcgc aatggtcggg cctggcagat
     2581 ggccggctgg cgccgctctt gggcaccgtg gtcgcgctgc tggcgctgat gctgatgtgg
     2641 aacctggcgc gccgcccgcg tgacccgcgc gcggtggcgc aggcgtacga cgccagcagc
     2701 gaccgctttt cgcaaaccgc ccccatcaat gaagaacagg tgcaactgct gcgttatctg
     2761 cagcaggcgt ttcctgatgg cgcggtgctg tttcggccgc gcctggcgcg ctttctgacg
     2821 gtgcgcaaaa gcagccaccg actgggcgct cagcagcgcc tggccgacgc gcaggtcgat
     2881 ttcctggttt gtgccgacga tggcaagccg ctgtttgcgt tcgaggtgga tgcgtttcgc
     2941 cacccggacg atgcggacat tgcgcgcaac gcggccgaga agaaccggat gctcaagagc
     3001 gccgacattc gcctgattcg cctgaaaggc gcgttgccga actggccgcc ggccgagata
     3061 ttgcgcatgc gcttgctggc ggcgcagcgc acgccggccg ccgaaggcag gccgtccggc
     3121 ttcagcccat cgggctttgg cccgtcaagc tttgcccaca ccgatttcgc gcattcgcgc
     3181 agcgccaatt cggacgtgat gggtctgtcc cgcctgatgg gcatgccggc cacggacgac
     3241 ccttgggaat cggtgcgcaa gcgctgagcg aggcgattta cttcaaaata gatagctgct
     3301 cgcgcaagag tatcgggcgc tggcggctgt ttttatctga aaaaggcgag tctgcagcgc
     3361 ttcagggcgc ctgctcgcgc agcttgaagc gctggatctt gccggtggcg gtcttgggca
     3421 attcgttcac gaactcgata aagcgcgggt atttgtaggg cgcgaggcgg tccttgacga
     3481 aggctttcag ttcgtcctcc gacgcctcgg cgccggcctt cagcaccacg aaggccttgg
     3541 tcttggtcag gccgtcggtg tcgggcttgc cgacgacggc agcttccagc acggcggggt
     3601 gctgcaccag cgtggcctcg acctcgaacg gtgacacata gatgccgctc accttcagca
     3661 tgtcgtctga gcggccgccg taggtgtagc tgccgtcgct gttgcgcacg tacttgtcgc
     3721 ccgacttggt ccagccgccc tggaaggtgt cgcgtgtctt gctgcggttg ccccagtaca
     3781 tcatggcggc gctggggccg gcgatgtaga ggtcgcccgg ctcgccgtcg ggcacggggc
     3841 cgccgtcgtc gccgcgcagc tcgacctcgt agcccggcac cggccagccg gtggtgccgt
     3901 agcgcacctt gtgcggcagg ttggacagga agatgtgcag catctcggtg gagccgatgc
     3961 catcgacgat gtcgacgccg aaatgcgcct tgaagcgttc gccgatctcg gccggcagtg
     4021 cttcgccggc cgacgagacc aggcgcatcg ccacgtcttc gcgtttgggc agcgcggggt
     4081 gcgccagcat gccggcaaag ccggtgggcg cgccgtagaa cacggtcggt ttcaggccgc
     4141 cgacttggcc cgtccagcgc ttgaaggtcg cctccggcgt gggccgctcg cccatcagca
     4201 gcgtggtggc gcccacgctc atcggaaagg tgagggcgtt gcccaggccg taggcaaaga
     4261 acagcttggc ggccgagaag cacacgtcgc tttcgcgcag atccagcacg cccttgccat
     4321 acaactcgca ggtccagtac gggttggcgt gcgaatgcac cgtgcccttc gggcggccgg
     4381 tggagccgct ggaatagagc cagaaggccg ggtcgtcggc gccagtggca gcgggcttgt
     4441 ccagctgcgc gtgggctttc aagaacgcct caaactcgac ctcggacggg tgcagcggcg
     4501 cctgcggttg cgagacgatc actttctgca cctcgtggtc gctcttgacc atcgccgccg
     4561 tcagagtcgg aagcagcgca cccgacacca gcaccgcctg ggcgcgcgaa tgctccagca
     4621 tgtaggcgta gtcgtcggcc gtgagcagcg tgttgacggc caccggcacc aggccggcgt
     4681 acagcgcgcc caaaaaagcc accggccagt cgcagccgtc gtgcatcagc accagcacgc
     4741 gttcttcgcg gcgcaaaaac aggcttttaa gcgccgacgc catgcggcgc gtgcgctcgg
     4801 ccagttcgcc ataggtaacc gtgccttgct catcgacgaa ggcaggcttg cccgcgcggt
     4861 tcgcgttgag cgcaagcaga tgctcggcaa agttgaagcg ctcggggggc ggggcgactt
     4921 cgggtgtcat ggcatgtctc cgggtttatc catcagtgca aacagccgga cgcgaaagtg
     4981 acgcgaaggc cgcgaaagaa aaagaaaaaa acctctgaat tctttcgcgt ccttcgcgta
     5041 aggctgtggg tcggctggtc ttctcgcgcc ccacattgat cttttgcagg tcgcgtggtg
     5101 aaatgaatta ttgtgcgtgt atcgtaagat gcattaaagt gcatgtcaag atgagttccc
     5161 catgagcact gaacttcaag aacgcccgcc gaccgacgcc gcgccggccg agccgccgcg
     5221 ccaccccttt cttgtcgcgc tgggcgagcg cgtacgcaac ctgcgctcgc gccgcggcat
     5281 gacgcgcaag gccgtggcgc aagcggccga ggtgtcagag cgacacctgg ccaacctcga
     5341 atacggcacc ggcaacgcct cgatcctggt gctgctgcag gtggcccagg cgctgcattg
     5401 cgacttgagc gagctgctgg gcgacgtgac cacctcgtcg cccgaatggc tgatgattcg
     5461 cgaactgctg gagcaccgca gcgagcccga tctgcgccgt gcgcgccagc agttgagcga
     5521 gttgttccac gcccccggcg ccgccgaccg cgcgcgcacc acgcgcatcg cggtgatcgg
     5581 cctgcgcggt gccggcaagt cggccctggg ccaacggctg gcgcaggatt tgggctacgc
     5641 cttcatcgaa ctcagccgcg agatcgagca gtttgccggt tgcagcatca acgaaatcca
     5701 caacctgtac ggcaccaacg cctaccgccg ctacgagcgc cgcgcgctgg aagaggcgat
     5761 ccagatctac cccgaagtcg tgatcgccac gcccggcggc ctggtggccg atgcggccaa
     5821 cttcaacctg cttttgcagc actgctacac catctggctg caggccgacc cgggcgacca
     5881 catgggccgc gtggcggcgc agggcgacat gcggccgatg gccgccagcg ccgaggcgat
     5941 ggaggatttg aagcgcatcc tggccgggcg ttcggctttc tacgccaagg ccgatctgcg
     6001 cttcaatacc agcgattacg acagcgagga ggcagccttc cgcgcgctgc gccagagcgt
     6061 gcaggaggcg acgggcatca aagcgtgaca tgcaaaataa tgcattgaca tggatgggaa
     6121 atgtgaatta ttattcatgt catccgatcc gatgagacaa cccgccaagg agccgccatg
     6181 cccgcccccc aagtcgaata ccgcaccgac cccagccaat atcgccacat caagctgtcg
     6241 tttgacggcg ccaccgcgca gctggccatc gacatcgacg aaaacgccgg cctgcgcccc
     6301 ggctacaagc tgaagctgaa cagctacgac ctgggcgtgg acatcgaact gcacgacgcg
     6361 ctgcagcgca tccgcttcga gcaccccgag gtcaagaccg tggtcgtcac cagcgccaag
     6421 gacaaggtgt tctgctcagg cgccaacatc tttatgctgg gcgtgtccag ccacggctgg
     6481 aaggtgaact tctgcaagtt caccaatgag acgcgcaacg gcatcgagga ctcgagccag
     6541 cacagcggcc tgaaattcat cgccgccgtc aacggcgcct gcgccggcgg cggctacgag
     6601 ttggcactgg cctgcgacga gatcatcctg gtcgacgacc gcaacagcag cgtcagcctg
     6661 cccgaagtgc cgctgctcgg cgtgctgccc ggtaccggcg gcctgacgcg cgtgaccgac
     6721 aagcgccatg tgcggcacga tttggccgac atcttttgca ccagcgtcga aggcgtgcgc
     6781 ggtcagcgcg cggtcgattg gcggctggtc gatgcggtgg ccaagccaca ggtgttcaac
     6841 gacaccgtgg cgaagcgggc cagcgaactg gcggccggca gccaccgtgg cgcgggccaa
     6901 ggcgtggcgc tcacgccgat cgagcgccag atcgacggcg acaccatcag ctataaaaat
     6961 gtaagcatcg acatcagccg tggcaagcgc aacgccacgt ttttggtcaa ggcgccgcag
     7021 ggcgatcaac cgaaagacat cgctggcatc gaagccgccg gcgtcaactg gtggccgctg
     7081 gccatggcgc gcgagctgga cgacgccatc ctgcacatgc gcaccaacga gctggacatc
     7141 ggcacctgga ttctgaaaac cgaaggcgac tcggctgcgg tgctggccgc cgacgccacg
     7201 atggaagcgc accgcgaaca ctggttcgtg aacgaaacca tcggcatgct gcgccgcacc
     7261 ttcgcgcggc tcgatgtgtc ctcgcgcagc ctgtttgcgc tgatcgagcc ggggtcgtgc
     7321 tttgccggca cactggccga gctggtgttc tgcgccgacc gcgcctacat gctcgatgac
     7381 gagaacgccg ccagcatcac gctggacagc ttcaactttg gcctgctgcc gatggtcaac
     7441 ggccagtcgc gcctggcgcg ccgcttttac gaagaagccg cgcccatgga agccgtgcgc
     7501 gccgccgccg gcaagccgct cggccccaag gaagccgaaa agctgggcct gatcaccgcc
     7561 gcgcccgacg acatcgactg ggccgacgaa gtgc
//
LOCUS       DAOMVO010000102         7482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2597_length_7482_cov_13.166420, whole genome shotgun sequence.
ACCESSION   DAOMVO010000102 DAOMVO010000000
VERSION     DAOMVO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_7482_cov_13.166420"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKV30_06555"
     CDS             complement(<1..187)
                     /locus_tag="PKV30_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRL29912.1"
                     /translation="MNSTDAKRILETALICAQQPMPLREFRVLFDDQVGPDTLKCLLA
                     ELQDDWAQRGVELVQVAS"
     gene            complement(316..1302)
                     /locus_tag="PKV30_06560"
     CDS             complement(316..1302)
                     /locus_tag="PKV30_06560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRL29913.1"
                     /translation="MADDGAGAESRSFALPAAQHGARLDRALADLVPEFSRNYLSQLV
                     ADGAVRLNGATATKPAHKVRAGDQLVIELRPTPMSQAFVPQAMDLNVLYEDEHLAVID
                     KPAGLVVHPAPGNWSGTLLNGLLARDAKAALLPRAGIVHRLDKDTSGLMVVARSRPAM
                     DALVARIAAREVRREYLALAHRAWSGPAEREVDAAIGRDPRNRLRMAVVDLDRGAGKP
                     AQTLFQLLQNTEQGCLVRCLLRTGRTHQIRVHMAHLGHPLVADALYGGAPAAGLTRQA
                     LHARRLAFAHPITGQALDFSAPLPADLAAALLAWGLTMPADDEMTRAAAAAR"
     gene            1389..2192
                     /locus_tag="PKV30_06565"
     CDS             1389..2192
                     /locus_tag="PKV30_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRL29914.1"
                     /translation="MQRARLSAASAGVLTLAALLAACSSKPVDKTAGWSPNRIHAEAK
                     DELNSGAYDKAIPLYESLEGRAAGTPLAQQAQLDKAYAQFRDGQKPEALATLDRFMRL
                     HPASPAIDYALYMKGVINFNDELGLFSFLSRQDLSERDQKAAKEAFESFNELVTRFPD
                     SRYAPDARARMRYTVNALAQYEVHVARYYYTRGAYVAAINRAQQAIADYRDAPALEEA
                     LYILTQSYDKLGMNDLRDDSKRVLDQNYPNSAYLTRGFRGADKPWWQIW"
     gene            complement(2480..4531)
                     /locus_tag="PKV30_06570"
     CDS             complement(2480..4531)
                     /locus_tag="PKV30_06570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRL29915.1"
                     /translation="MSSTPLAQAVQSTFAPGGPLAQAAEGFVARDGQTQLAQAVARTI
                     GQGGVLVAEAATGVGKTFAYLVPALLSGERVLVSTATKTLQDQLFGRDLPRLARALGV
                     PARMALLKGRSSYLCLHRLEFAGESSDLRMTGARHALSKIERWSKTTRTGDLAELPGL
                     DERSPVIPLVTSTRENCLGADCPKFRACHVNLARREAMAADVVVINHHLFFADLAIRE
                     SGMAELLPTVRVAIFDEAHQLNETGIQFLGQQLATGQLLDFARDVLGAGLQQARGLCD
                     WTQLASGVEMAARDLRMAVGQVRGASRLRWTAEAPDGVEAVAWQASLARLHTALTDAA
                     AALDTVSELAPDFVRLHERALALAARVQRFAAPCDVALVRWADVSQSLRLVESPLDIA
                     DTVRTRLLAAPEGEGPARAWVFVSATLGDDARLSWFTQPCGLQDAEVLRIGSPFDYAR
                     QAALYVPAHIARPADPAHSGDVARLAGDAAARLGGRTMVLTTTLRALRSIGDALAERF
                     PVSSGIEVLVQGALPKRVLMDRFRAGNAGGQRGCVLVASASFWEGVDMPGDALQLVII
                     DKLPFPPPGDPLVEARCRRIESEGRSAFADYSVPEAAVALKQGAGRLIRRETDTGLLV
                     ICDTRLTQMGYGRRLLAALPPMRRITNPAEFDAALAALRDAPVGEQGNWASNRPPAHV
                     Q"
     gene            complement(4528..4731)
                     /locus_tag="PKV30_06575"
     CDS             complement(4528..4731)
                     /locus_tag="PKV30_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRL29916.1"
                     /translation="MTANLHSPERQLIELRIEHADLDASIDRLAEQSPVDELMLQRLK
                     KRRLALRDAMARLEAALDPKEPA"
     gene            4822..5622
                     /locus_tag="PKV30_06580"
     CDS             4822..5622
                     /locus_tag="PKV30_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRL29917.1"
                     /translation="MEKGYRLSAATGLHKGDREYQQDQVGLFAHPRVTGCVMGVVADG
                     MGGRTGGRKASDQVLLTSRQLFERYAPDSEDAAAVLRQIGQEAHLVVKLTAIAAEQEP
                     HTTMAAFLINPGGECHWVHAGDSRLYHFRGATLVRRTKDHSYVQALVDQGEMTEEEAA
                     IHPKSNVLLGCLGTEEEPRFEPHFIPQLQIGDSLLACSDGLWHYFTTEELGSVLNSLP
                     PREASEFLVDKARARAMGGGDNLSLAIVKVEPLVVNKPVVGSAFAPLR"
     gene            complement(5664..6077)
                     /locus_tag="PKV30_06585"
     CDS             complement(5664..6077)
                     /locus_tag="PKV30_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29918.1"
                     /translation="MKRLFACLSLCLLLAACASPSLHDERADERAADAAAAQALAASA
                     PPVSEALDPWLQAQRARIETARADATQRFEADEKACWRRFAVNACVRAARADRRSVTD
                     RLRQEELAVNEVERQRTSQARLRQLEEKQRSAAPQ"
     gene            complement(6074..7174)
                     /gene="zapE"
                     /locus_tag="PKV30_06590"
     CDS             complement(6074..7174)
                     /gene="zapE"
                     /locus_tag="PKV30_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRL29919.1"
                     /translation="MSSVREAYQADLKARGYQSDPAQLRAIDALQRCADDWAAYKDKR
                     SNSLKKLFNKPDIPKGVYMYGGVGRGKSFLMDCFYNAVPIRRKTRLHFHEFMREVHRE
                     LAGLKGTVNPLDELGKRMARKFKLICFDEFHVADITDAMILHRLLAALFDNGVGFVTT
                     SNFKPDDLYPGGLHRERILPAIELLNQRMEVVNVDNGTDYRRRTLEQVELYHTPLGPE
                     ADAAMNEAFTRLASGGDEDPVLHIEAREIKARRRAGGVVWFDFKQLCGGPRSQNDYLE
                     IASQFHTLLLSDVPHMPVRMASEARRFTWLVDVLYDRRVKFILSAEVAPELLYTEGPL
                     AHEFPRTVSRLNEMQSAEYLALPHRTVDTQLT"
     gene            complement(7247..>7482)
                     /locus_tag="PKV30_06595"
     CDS             complement(7247..>7482)
                     /locus_tag="PKV30_06595"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="E3 component of 2-oxoglutarate dehydrogenase
                     complex; catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRL29920.1"
                     /translation="LGDTTGMVKFLADAATDEILGVHIVGPMASELIAEACVAMAFRA
                     SSEDIARICHAHPSLSESTKEAALAVDKRTLNF"
BASE COUNT         1185 a         2563 c         2548 g         1186 t
ORIGIN      
        1 cgctggccac ctgcaccagc tcgacgccgc gctgcgccca gtcgtcctgc agctcggcca
       61 gcaggcattt cagcgtatcg gggccgacct ggtcgtcaaa cagcacgcga aactcgcgca
      121 gcggcatggg ctgctgcgcg cagatcaggg cggtttcaag aatgcgtttg gcgtccgttg
      181 agttcacggc gtgggtgcaa aagggatgtg cggggcgacc ggcgtgcgct gggtgccgaa
      241 tgacgaaatg acttcgctgc ggcgcagcgt caatgacgga atgaccagca ccggatcggc
      301 ggttgagtca ttttgtcatc gcgcggccgc agccgcgcgg gtcatttcgt catcggcagg
      361 cattgtaagt ccccatgcca gcagcgctgc cgccaggtcg gccggcagcg gcgcgctgaa
      421 atccagcgcc tggccggtga tcgggtgcgc gaacgccagc cggcgcgcgt gcagcgcctg
      481 ccgcgtcaac ccggctgcag gcgcgccgcc gtacagcgcg tcggccacca gcgggtgccc
      541 gagatgggcc atatgcaccc ggatctggtg cgtgcggccg gtgcgcagca ggcagcgcac
      601 caggcagccc tgctccgtgt tttgcagcag ctgaaacaag gtctgcgccg gcttgcccgc
      661 gccccggtcc agatcgacga ccgccatgcg caggcggttg cgcgggtcgc ggccaatcgc
      721 ggcatcgacc tcgcgctcgg ccgggccgct ccaggcgcgg tgcgccagcg ccaggtattc
      781 gcgccgcacc tcgcgcgcgg cgatgcgcgc caccagcgca tccatcgcgg gacgcgagcg
      841 ggcgaccacc atcagcccgc tggtgtcctt gtcgagccgg tgcacgatgc cggcgcgcgg
      901 tagcagtgcg gccttggcgt cgcgcgccag caggccgttg agcagcgtgc cgctccagtt
      961 tccgggcgcg gggtgcacca ccaggccggc cggcttgtcg atgacggcca agtgctcgtc
     1021 ttcgtacagc acgttcaagt ccatcgcctg cggcacgaag gcctggctca tcggcgtggg
     1081 gcgcagctca atgaccagct gatcgcccgc gcgcaccttg tgcgccggct tggtcgccgt
     1141 ggcgccattc aggcgcacgg cgccgtcggc gaccagctgg ctcaggtagt tgcgcgagaa
     1201 ctcgggcacc aagtcggcca gcgcccgatc cagccgcgcg ccgtgctgtg cggccggcag
     1261 cgcaaagctg cgcgactcgg cgccggcgcc gtcgtcggcc agctcgtcgt cggcctctgg
     1321 cgcgggtacg gccaaaaaag ggcccgccga tataatggaa tgtgcttgat tcaacaggaa
     1381 tgcgactgat gcaacgtgcc cgattatcgg ccgccagcgc gggggttttg acattggccg
     1441 cactgctggc ggcttgctcg tccaagccgg tcgacaagac cgccggctgg agccccaacc
     1501 gcatccacgc cgaagccaag gacgagctca attccggcgc ctacgacaag gcgatcccgc
     1561 tgtacgagtc gcttgaaggc cgcgctgccg gcacgccgct ggcgcagcag gcgcagctcg
     1621 acaaggccta cgcccagttc cgcgacggcc agaagcccga ggccctggcc acgctcgacc
     1681 gcttcatgcg cctgcacccg gccagcccgg cgatcgatta cgcgctgtac atgaagggcg
     1741 tgatcaactt caacgacgaa ctggggctgt tctccttcct gtcgcggcag gacttgtccg
     1801 agcgcgacca gaaggcggcc aaggaagcgt ttgaatcgtt caacgaactg gtcacgcgct
     1861 ttcccgattc ccgctacgcg ccggacgccc gcgcgcgcat gcgctacacc gtcaatgcgt
     1921 tggcgcaata cgaagtgcac gtggcgcgct actactacac gcgcggcgcc tacgtggcgg
     1981 ccatcaaccg cgcgcagcag gcgattgccg actaccgcga cgcgccggcg ctggaagagg
     2041 cgctgtacat cctcacccag tcgtacgaca agctcggcat gaacgatctg cgcgacgaca
     2101 gcaagcgcgt gctcgaccag aactacccga acagcgcgta tctgacgcgc ggcttccggg
     2161 gggcggacaa gccgtggtgg caaatttggt gacatccccc tgagcggctg acgccgcttc
     2221 ccccttctct cgcttcgctg cgctacccgg gaagggggac gcacccagcg ggcgggccaa
     2281 gccttccgcg ggcgcccgcg cgtggcctgc tccgcggccg tttgagttgc tactgtttgg
     2341 atagctgctg gcgcttgata gacgtgcgct gcggcccaat ttaccctgtg gaagcggcct
     2401 tggccgcgat ggagcgattg atctggagct gacgcaccat cgcgggcaag cccgctccta
     2461 cgacgatagc tgcttgcgct tactggacgt gcgctggcgg cctatttgat gcccaattgc
     2521 cctgctcgcc gaccggagca tcgcgcagcg ccgccagcgc ggcgtcgaac tcggcgggat
     2581 tcgtgattcg ccgcatcggc ggcagcgcgg cgagcaggcg gcggccgtag cccatttggg
     2641 tcaggcgggt gtcgcagatg accagcaggc cagtgtcggt ttcgcggcgg atcaggcggc
     2701 cggcgccttg cttcaaggcc acggccgcct cgggcaccga atagtcggca aaggcgctgc
     2761 ggccctcgct ttcgatgcgg cggcagcgcg cctcgaccag cggatcgccc ggaggcggaa
     2821 aaggcagctt gtcgatgatg accagctgca gcgcgtcgcc gggcatgtcg acgccttccc
     2881 agaacgaggc cgaggcgacc agcacgcacc cgcgctgacc gcccgcgttg ccggcgcgaa
     2941 agcggtccat cagcacgcgc ttgggcaaag ccccttgcac cagcacctcg atgcccgagc
     3001 tgaccggaaa gcgctccgcc aacgcgtcgc caatgctgcg cagcgcacgc agcgtggtcg
     3061 tcagcaccat ggtgcgcccg cccaggcgcg ctgcggcatc gccggccaga cgcgccacgt
     3121 cgccgctgtg cgccgggtcg gccgggcgcg cgatgtgggc gggcacgtac agcgcggcct
     3181 ggcgcgcgta gtcgaacggg ctgccgatgc gcagcacctc ggcgtcctgc aggccgcacg
     3241 gctgcgtgaa ccacgacagg cgcgcgtcgt cgcccagcgt ggcagagacg aacacccagg
     3301 cgcgcgccgg cccctcgcct tccggcgccg ccagcaagcg cgtgcgaacc gtatcggcga
     3361 tgtcgagcgg cgattcgacc aggcgcagcg actggctgac atcggcccag cgcaccagcg
     3421 ccacgtcgca cggcgccgcg aaacgctgca cacgcgcagc cagcgccagc gcgcgctcgt
     3481 gcaggcgcac gaagtcgggg gccagttcgc tcacggtgtc gagcgcggcc gcggcatccg
     3541 tcagtgccgt gtgcagacgc gccaggctgg cctgccacgc gacggcctcc acgccatcgg
     3601 gcgcttcagc cgtccagcgc aggcggctgg cgccgcgcac ctggcccacg gccatgcgca
     3661 ggtcgcgcgc ggccatctcc acgccgctgg ccagttgcgt ccagtcgcac aggccgcgcg
     3721 cctgctgcag gccggcaccg agcacgtcgc gcgcgaagtc gagcagttgg cccgtcgcca
     3781 gttgctgtcc gagaaactga atgcccgtct cgttgagctg gtgcgcctcg tcgaagatcg
     3841 ccacgcgcac cgtgggcagc aattcggcca tgccagattc gcggatcgcc aggtccgcaa
     3901 agaacaggtg gtggttgatg accaccacgt cggccgccat ggcttcgcgc cgcgccaggt
     3961 tgacgtggca ggcgcggaac ttggggcagt cggcgcccag gcagttctcg cgcgtgctgg
     4021 tgaccagcgg gatcacgggc gagcgttcgt ccagccccgg cagttcggcc aggtcaccgg
     4081 tgcgcgtggt tttcgaccat cgctctattt ttgagagcgc gtgacgcgcg cctgtcatgc
     4141 gcaaatcgct gctttcgccc gcaaactcca gccggtgcag gcacaggtag ctgctgcgcc
     4201 ctttcagcag cgccatgcgc gcgggcacgc ccagtgcacg cgccaggcgg ggcagatcgc
     4261 ggccgaacaa ctggtcttgc aaggtcttgg tggccgtcga aaccagcacg cgctcgcccg
     4321 acagcagcgc aggcaccaga taggcaaagg tcttgccgac gccggtggcg gcctcggcca
     4381 ccagcacgcc gccttgcccg atggtgcgcg ccaccgcctg cgccagttgc gtctggccgt
     4441 cgcgcgcgac aaagccctcg gccgcctgcg ccagcgggcc gccaggggca aaggtggatt
     4501 gcaccgcctg ggccagcggc gtgctgctca tgcgggctct ttgggatcga gcgcggcttc
     4561 gagccgcgcc atcgcgtcgc gcagggccag gcggcgtttc ttgagccgct gcagcatcag
     4621 ctcgtcgacc ggcgattgct cggccaggcg gtcgatcgag gcgtcgagat cggcgtgctc
     4681 gatgcgcagc tcgatcagtt gccgttcggg ggaatgcagg ttggccgtca cttcaaagct
     4741 acaaaaaaga aagtcgcgcg cgacttccgt tcaataatac ggcttcgacg tccacccacg
     4801 gccaccgtgc ccgatgaaaa catggaaaaa ggctatcgac tcagcgccgc caccggactg
     4861 cacaagggcg accgcgaata ccagcaggat caggtcggct tgttcgcgca cccgcgcgtg
     4921 accggctgcg tgatgggcgt ggttgccgac ggcatgggcg ggcgcaccgg cggccgcaag
     4981 gcgtcggacc aggtgctgct gacctcgcgc cagctcttcg agcgctatgc ccccgatagc
     5041 gaagacgcgg cggcggtgct gcgccagatc ggccaggagg cgcatctggt ggtcaagctg
     5101 acggccatcg ccgccgagca ggagccccac accaccatgg cggctttcct catcaacccg
     5161 ggcggtgaat gccactgggt gcacgccggc gattcgcgcc tgtaccactt tcgcggcgcc
     5221 acgctggtcc ggcgcaccaa ggaccattcc tacgtgcagg cgctggtcga ccagggcgag
     5281 atgaccgagg aagaagcggc gatccacccg aagtccaacg tgctgctggg ttgcctgggc
     5341 accgaggaag agccgcgctt cgagccgcat ttcatcccgc aactgcagat tggcgattcg
     5401 ctgctggcct gcagcgacgg gctgtggcat tacttcacca ccgaagagct gggttcggtg
     5461 ctcaactcgc tgccgccgcg cgaggcgagc gagttcctgg tcgacaaggc acgcgcgcgc
     5521 gccatgggcg gcggcgacaa cctgtcgctg gccatcgtca aggtggagcc gctggtggtc
     5581 aacaagccgg tggtcggctc ggccttcgcg ccgttgcgct gagtgcgtca cgccgctgcc
     5641 caccctgggc ggcggcgctt caactattgc ggcgcggcgc tgcgctgttt ttcttccagc
     5701 tgacgcaggc gcgcctggct ggtgcgctgg cgctccacct cgttcacggc cagctcttcc
     5761 tggcgcagcc ggtcggtcac gctgcgccgg tcagcgcgcg cggcacgcac gcaggcgttg
     5821 acggcaaagc gccgccagca ggctttttca tcggcttcga aacgctgcgt tgcgtcggcg
     5881 cgcgcagtct cgatgcgcgc acgctgcgcc tgcagccacg gatcgagcgc ttcgctgacg
     5941 ggcggcgcgc tcgcagccaa tgcctgcgcg gcggcggcat cggccgcgcg ctcgtcagcg
     6001 cgttcgtcgt gaagcgaggg cgacgcgcaa gcagccaaca acaagcacag cgacaggcag
     6061 gcgaacaggc gtttcatgtc agttgagtat cgacggtgcg gtggggcagc gcaaggtatt
     6121 cggccgactg catctcgttc agccggctca cggtgcgcgg aaactcatgc gccagcgggc
     6181 cttcggtgta cagcagctcg ggcgccactt cggccgacag gatgaacttg acgcgccggt
     6241 cgtacagcac gtcgaccagc caggtgaagc ggcgcgcttc cgacgccatg cgcactggca
     6301 tgtgcggcac gtcggacagc agcagggtgt gaaactgact ggcgatttcc aggtagtcat
     6361 tctgcgagcg cgggccgccg cagagctgct tgaaatcgaa ccacaccacg ccgcccgcgc
     6421 gccggcgcgc cttgatctcg cgcgcctcga tgtgcagcac cgggtcctca tcgccgcccg
     6481 aggccaggcg cgtgaaggcc tcgttcatgg cggcgtcggc ttcgggcccg agcggcgtgt
     6541 gatacagctc gacctgctcc agcgtgcgac ggcggtagtc ggtgccgttg tcgacgttga
     6601 ccacttccat gcgctggttc agcaactcga tggcaggcag gatgcgctcg cggtgcaggc
     6661 cgccggggta caggtcgtcg ggcttgaagt tgctggtggt gacaaagccc acgccgttgt
     6721 cgaacagcgc cgccagcagg cggtgcagga tcatggcgtc ggtgatgtcg gcgacgtgga
     6781 actcgtcgaa gcagatcagc ttgaacttgc gcgccatgcg cttgcccaat tcgtccagcg
     6841 ggttgacggt gcccttcagc ccagccagtt cgcggtgcac ctcgcgcata aattcgtgaa
     6901 agtgcagccg cgtcttgcgg cggatcggca cggcgttgta gaagcaatcc atcagaaagc
     6961 tcttgccgcg gccgacgccg ccgtacatgt acacgccctt ggggatgtcg ggcttgttga
     7021 acagcttctt caagctgttc gagcgcttgt ccttgtacgc cgcccaatcg tccgcgcagc
     7081 gctgcagcgc gtcaatggcg cgcagttgcg ccgggtcgct ctggtagccg cgcgccttca
     7141 ggtcggcctg gtaggcttca cgcacactgc tcacggccgc tcaaccgata ctattatttt
     7201 aatagctgct tgcgcttgat acacaagcgc tgcagcccgt tttttattag aagttgagcg
     7261 tgcgcttgtc caccgccagc gccgcttcct tggtcgattc gctcaaggat gggtgcgcgt
     7321 ggcagatgcg ggcgatgtct tcgctgctgg cacggaaggc catcgccacg caggcctcgg
     7381 cgatcagctc ggacgccatg gggccgacga tgtgcacgcc cagaatctcg tccgtcgccg
     7441 catcggccag gaacttgacc atgcccgtgg tgtcgcccag cg
//
LOCUS       DAOMVO010000103         7426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2629_length_7426_cov_16.548230, whole genome shotgun sequence.
ACCESSION   DAOMVO010000103 DAOMVO010000000
VERSION     DAOMVO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_7426_cov_16.548230"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKV30_06600"
     CDS             complement(<1..850)
                     /locus_tag="PKV30_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29921.1"
                     /translation="MSNLWDLSHIQPQPSSVLAGDTIAAMFWNAVAQRGPDVWLRKKD
                     LGIWRSWTWQQTGEAAREIAGGLMQLGFAAGDTASILSNTVVEWVMADLAVLSCGGVS
                     NGIYPTDAASQVQYLCEDSRTSVLFVEDDEQLDKALEVRAQLPGLKKIVVFDMKGLRD
                     LDDAGVISLDDLRALGREYNQQHPDELPRRVAALRPDDVAILVYTSGTTGKPKGAMHT
                     HGGLVYTVRGYNTLISRDQADECMCFLPLCHIAERMGGEYFSLYTGARLNFVENPDTV
                     PENVREI"
     gene            complement(853..1668)
                     /locus_tag="PKV30_06605"
     CDS             complement(853..1668)
                     /locus_tag="PKV30_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29922.1"
                     /translation="MNATQTIAASADPSSAGAILKLNNVESAYGPIKAIRGVSLQVRQ
                     GEIATVLGSNGAGKSTILKTISGIIDPRKGSIEFKGADITARDPAYVVQQGLSHVPEG
                     REVFPLMSVRENLLMGAYTRKDRDGVARDIEQVFSYFPILKERMSQDAGLLSGGQQQM
                     LAISRALMAKPDLMLLDEPSLGLSPKLTKEIFEIVVRINRERGTTILLVEQNANMALN
                     AADYGYVLENGRIVMEDTCERLREKEDIKEFYLGMKDEGVRGERRWKKKKTWR"
     gene            complement(1665..2480)
                     /locus_tag="PKV30_06610"
     CDS             complement(1665..2480)
                     /locus_tag="PKV30_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL29923.1"
                     /translation="MSDILLSAKNLSVRFGGVLAVNNVSFDVRRGEVFTLIGPNGAGK
                     TTVFNLISRIYTPTSGEIDYAGPQGTVKLTDQPPHRVASLGIARTFQNIELFEHATVL
                     HNLLIGRHTHRQTGLWSEMFFTPATRRAELQAREHVERVIDLLDLQHHRDSMVAGLPY
                     GVRKVVELARALCAEPRLLLLDEPSSGLNVEETEDMAFWIQDIVHELGITVLMVEHDM
                     TLVSRVSDRVVAMNQGQVLAVGTPREVQSDPGVVEAYLGSIDDVSSLRRVAAQ"
     gene            complement(2477..3553)
                     /locus_tag="PKV30_06615"
     CDS             complement(2477..3553)
                     /locus_tag="PKV30_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL29924.1"
                     /translation="MRFIFKTDYDQDIRLAKHGGQVFWYSALLLFMLAAPWLLPEYWL
                     AQLTFVLIYAVIGVGLMLLAGFTGQFSLGHAAFVGVGAYAQAVLTNAGLPFPLAMVCA
                     ALLAAAAGVVVGLPALRLKGIYLGIATLAFGFIVEEIFARWESVTGGNAGMHVKAPAI
                     FGWTIDSGEGFYFICLITAVLATLAIVNLLRSSTGRAFVAIRDSEVSAQSMGIHLARY
                     KTLAFALSAALAGIGGALYAHKMRFLSPDQFSIIQSIDLLLMVVIGGLGSVHGAFLGA
                     IFLIAMPQIISMVKDYLPDAIGQAPGLQAVVYGSVLIAFVLFEPLGLYGRWLKVRTWL
                     QMFPFYRRGMFKRQKSFQKSDRLK"
     gene            complement(3557..4432)
                     /locus_tag="PKV30_06620"
     CDS             complement(3557..4432)
                     /locus_tag="PKV30_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL29925.1"
                     /translation="MQFLQLVISGIAQGCIYGLIALGFVLIYKATETVSFAQGDLMML
                     GAFAGLAGMTMLGFPFWLAVISAIGAMALLGLVTERLVIRPILGQPAFSIVMLTIGLG
                     YVGRGLITMIPGIGTETHTLPVPYKDQSWTLGGLVVSMEQTAVIVVTAVLCAALYALF
                     KYSKLGLAMQASSQNQLAAYYMGIPVKRLNGLVWALAAAVAAIAGLLLAPITFVHANM
                     GFIGLKAFPAAVVGGFGSLPGAIVGGLVIGIVESLSGFYLPEGFKDIAAYIVVLIMLM
                     VKPNGLFGEKLRKKV"
     gene            4564..5364
                     /locus_tag="PKV30_06625"
     CDS             4564..5364
                     /locus_tag="PKV30_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRL29926.1"
                     /translation="MTVELEKQGDVTVVTLVRPDVRNAVDGVTAQALHQAFVDFDQDD
                     TARVAVLHGAHGHFCAGWDLKSGAEMARDSAVHVDALLSQLDYASADAQPLGPMGPSR
                     LLLSKPVIAAIEGAAVAGGMELALWCDLRVMAEDAYFGIYCRRFGVPLIDGGTVRLPR
                     LIGQSRALDLFLTGRRVDAPEALAMGLANRMVGAGQALDAAVALARQIAAFPQDTLRA
                     DRLSALTQWDWPLAEALHREWTRGSQRLADALQGAARFASGAGRRGEF"
     gene            5473..6678
                     /locus_tag="PKV30_06630"
     CDS             5473..6678
                     /locus_tag="PKV30_06630"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRL29927.1"
                     /translation="MTEAYVFDAIRTPRGKGKKDGSLHEVKPVDLLAGTLTALKERHD
                     FDTAAVDDVVMGVVSPIGEQGSVIAKVAALKAGWDWQCAGVQLNRFCASGLEAVNMAA
                     QKVRSGWEDLVVAGGVESMSRVPIGADGGAWAQDPATNSATDFVPQGVGADLIATLEA
                     FSREDVDRFAVTSQQRAAAAREAGRFKGSVVPVTDFLGQTILEEDEFIKPRTTIEALA
                     GLKPSFEQLGAMGFDAVAISRYPQVERITHVHHAGNSSGIVDGAAAVLIGNEAAIKTH
                     GFKPRARIVATALSGADPTIMLTGPMPATRKALAKAGLKIEDIDLFEVNEAFAAVPMK
                     FMKEFNVPHEKVNVNGGAIALGHPLGATGAIILGTLIDELHRRHLRYGLATLCVGGGM
                     GIATIVERI"
     gene            6739..>7426
                     /locus_tag="PKV30_06635"
     CDS             6739..>7426
                     /locus_tag="PKV30_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL29928.1"
                     /translation="MKLKTLRYALTDGIASITFDEENSPVNTMCLQWQDDLQTVTDQV
                     VKDKDAIRGIVLASAKKTFFAGADLKGVMRMTGADAPKVFAEIERIKKNFRTLETLGK
                     PVVSCLNGAALGGGWEVALVGHHRIAVSDPKIQFGLPEVTIGLMPGGGGITKMTRLLG
                     LMGAQPYVLESKLFNPIEAQQMGVVHQLVDSADELIPAARAWIESVQGDADAATHPWD
                     RKDYKMPGGTP"
BASE COUNT         1344 a         2429 c         2432 g         1221 t
ORIGIN      
        1 cgatctcgcg cacgttctcg ggcacggtgt cggggttctc gacgaagttg agccgggcgc
       61 cggtgtagag cgaaaaatac tcgcccccca tacgctcggc gatgtggcac agcggcagaa
      121 agcacatgca ttcgtcggcc tggtcgcgcg agatcagcgt gttgtagccg cgcacggtgt
      181 agacgagtcc cccgtgcgtg tgcatcgcgc ccttgggctt gccggtggtg cctgatgtgt
      241 agaccaggat cgccacgtcg tcgggccgca gcgccgccac gcggcgcggc agttcgtccg
      301 gatgctgctg gttgtattca cgacccagcg cgcgcagatc gtccaggctg atcactccgg
      361 cgtcgtccag gtcgcgcagg cccttcatgt cgaacacgac gatcttcttc aggcccggca
      421 gttgcgcgcg cacctccagc gccttgtcga gctgctcgtc gtcttcgaca aacagcaccg
      481 aggtgcgcga gtcttcgcac aggtactgca cctggctggc cgcgtcggtc gggtagatgc
      541 cgttcgatac gccgccgcaa gacaacacgg ccaagtcggc catcacccat tcgaccaccg
      601 tgttcgaaag gatggaggcc gtgtcgcctg ctgcaaagcc cagctgcatc aagccgccag
      661 caatttcgcg agccgcctcg ccggtctgct gccaggtcca gctgcgccag atgcccaagt
      721 ctttcttgcg cagccacaca tccgggccgc gctgggccac ggcgttccag aacatggcgg
      781 caatggtgtc gccggcgagc acgctgctgg gctgcggctg gatgtgtgaa aggtcccaaa
      841 gattcgacat gcttatctcc aggtcttctt cttcttccac cggcgctcgc cgcgcacacc
      901 ctcatccttc atgccgaggt agaactcctt gatgtcttcc ttctcgcgca ggcgctcgca
      961 ggtgtcttcc atgacgatgc ggccgttttc cagcacatag ccatagtcgg cggcgttgag
     1021 cgccatgttg gcgttctgct cgaccagcaa gatggtggtc ccgcgctcgc ggttgatgcg
     1081 caccacgatc tcgaaaattt ccttggtcag cttcgggctc aggcccaggc tgggctcgtc
     1141 cagcagcatc aggtccggct tggccatgag cgcgcggctg atcgccagca tttgttgctg
     1201 gccgccggag agtaggccgg catcttgcga catgcgctct ttcagaatgg ggaagtaact
     1261 gaacacctgc tcgatgtcgc gcgccacgcc gtcgcggtcc ttgcgggtgt aggcgcccat
     1321 cagcaggttc tcgcgcaccg acatcagcgg aaacacctcg cgcccctcag gcacgtgcga
     1381 cagaccttgc tgcacgacat aggccgggtc gcgcgcggtg atgtcggcgc ccttgaactc
     1441 gatgctgccc ttgcgcggat cgatgatgcc ggagatggtt ttgaggatgg tgctcttgcc
     1501 cgcgccgttg ctgccgagca ccgtggcgat ctcgccctgt cgcacctgca ggctgacgcc
     1561 gcgaatggcc ttgatcgggc cataggcgct ctcaacgttg ttgagcttca aaatggcccc
     1621 agcgcttgat ggatcagcgc tggcagctat tgtttgagta gcgttcattg cgccgccacc
     1681 ctccgcaaag aggacacgtc gtcaatcgaa cccagatacg cctcgacgac ccccgggtcg
     1741 ctctgcacct cgcgcggcgt gcccacggcc agcacctggc cctggttcat ggccaccacg
     1801 cggtcggaca cgcgcgacac cagcgtcatg tcgtgctcca ccatcagcac ggtgatgccc
     1861 aactcgtgca cgatgtcctg aatccagaac gccatgtctt cggtttcctc gacgttcagg
     1921 cccgacgatg gctcgtccag cagcagcagg cgcggctcgg cgcacagcgc gcgcgccagc
     1981 tcgaccacct tgcgcacgcc gtaaggcagg ccggccacca tcgagtcgcg gtggtgttgc
     2041 agatcgagca ggtcgatcac gcgctcgacg tgttcgcgcg cctgcaactc ggcgcggcgc
     2101 gtggcgggcg tgaagaacat ctcgctccac aaccccgtct ggcggtgtgt gtggcggccg
     2161 atcagcaggt tgtgcagcac cgtggcgtgc tcgaacaact cgatgttctg gaacgtgcgc
     2221 gcgatgccga gcgaggcgac ccggtgcggc ggctggtcgg tgagcttgac cgtgccttgc
     2281 gggccggcgt agtcgatctc gccgctggtg ggcgtgtaga tgcggctgat gaggttgaac
     2341 accgtagtct tgccggcgcc gttgggaccg atgagcgtga acacctcgcc gcgccgcacg
     2401 tcgaagctga cgttgttgac cgccagcacg ccgccgaagc gcacgctcag gtttttggct
     2461 gaaagcagga tgtcgctcat ttgaggcgat ccgacttttg aaaggacttc tgccgcttga
     2521 acatgccacg gcggtagaac gggaacatct gcagccaggt gcgaaccttc agccagcggc
     2581 cgtacagccc cagcggctcg aacagcacaa acgcgatcag caccgaaccg tagacaacgg
     2641 cctgcaagcc cggcgcctgg ccaatggcgt cgggcaggta gtccttcacc atcgagatga
     2701 tctggggcat ggcgatgaga aagatggcgc ccaggaacgc gccatgcacc gagcccagcc
     2761 cgccgatcac caccatcagc agcagatcga tcgactggat gatgctgaac tgatcgggcg
     2821 acagaaaacg catcttgtgc gcgtacagcg cgccgccgat gccggccagc gcggccgaca
     2881 gcgcgaacgc cagcgtcttg tagcgcgcca ggtgaatgcc catgctctgc gccgacacct
     2941 ccgaatcgcg gatggcgaca aaggcgcggc cggtggaaga gcgcagcagg ttgacgatgg
     3001 ccagcgtcgc cagcacggcg gtgataaggc agatgaaata aaaaccttcg cccgaatcga
     3061 tcgtccagcc gaagatggcc ggagccttga cgtgcatgcc ggcgttgccg ccggtcacgc
     3121 tttcccagcg tgcaaagatc tcctcgacga tgaagccgaa cgccagcgtg gcgataccga
     3181 ggtagatgcc cttcaggcgc agcgccggca gccccaccac cacacccgcc gccgctgcca
     3241 gcagcgcggc gcacaccatg gccagcggaa acggcagccc ggcattggtc agcaccgcct
     3301 gcgcgtacgc gcccacgccc acgaaggcgg cgtgcccgag cgaaaactgc cccgtgaagc
     3361 ccgccagcag catcagcccg acgccgatca cggcatagat caacacgaag gtcagctgcg
     3421 ccagccagta ttcgggcagc agccagggcg ccgccagcat gaacagcagc agcgccgaat
     3481 accagaacac ctgtccgcca tgcttggcca ggcgaatgtc ctggtcgtag tcggtcttga
     3541 agatgaagcg catcgttcag accttcttgc ggagtttctc gccgaacagg ccgttgggtt
     3601 tgaccatcaa catgatcagc accacgatgt acgccgcaat gtccttgaag ccttcgggca
     3661 agtaaaagcc ggacagcgat tcgacgatgc cgatgaccag cccgcccacg atggcgcccg
     3721 gcaggctgcc aaagccgccc accacggcgg cgggaaaagc cttcaggccg atgaagccca
     3781 tgttggcgtg cacgaaggtg atgggcgcga gcagcaggcc ggcaatcgcc gccaccgctg
     3841 cggccagcgc ccacaccagc ccgttcaggc gcttgaccgg aatgcccatg taataggccg
     3901 ccaactggtt ctgcgacgag gcctgcatgg ccaggcccag cttgctgtac ttgaacagcg
     3961 catacagcgc ggcgcacagc acggcggtga cgacgatcac cgccgtctgc tccatgctga
     4021 cgaccaggcc gcccagcgtc caggactgat ccttgtaggg cacgggcaag gtgtgcgtct
     4081 cggtgccgat gccgggaatc atggtgatca gcccgcggcc gacataaccc aggccaatgg
     4141 tcagcatgac gatcgagaac gccggctggc cgaggatggg gcgaatcacc agccgctcgg
     4201 tcaccagccc cagcagcgcc atggcgccga tggccgagat caccgccagc cagaacggaa
     4261 agcccagcat cgtcatgccg gccaggccgg caaaggcgcc cagcatcatc aggtcgccct
     4321 gcgcgaagct gacggtttcg gtggccttgt agatcagcac aaagccgagc gcgatcaggc
     4381 cgtagatgca gccttgcgcg ataccgctga tgaccaattg cagaaactgc acgtgcctgg
     4441 cgcccgccgg ggcggggagg ttcatgaaag atgcccgact ctaatcagca ccgcgcgcgc
     4501 cgggtgcccc gaaaaaccct aagctgtcgc atgggcgata gcccgcgttc aagggcactg
     4561 gggatgacgg ttgagctgga aaagcagggc gatgtgaccg tggtcacgct ggtgcggccg
     4621 gatgtgcgca acgctgtcga cggtgtcacg gcgcaggcgc tgcaccaggc gtttgtcgat
     4681 ttcgatcagg acgacaccgc gcgcgtggcc gtgctgcatg gcgcgcacgg tcacttctgc
     4741 gccggctggg atctgaaatc cggcgccgaa atggcgcgcg actcggcagt ccatgtggat
     4801 gcgctgctgt cccaattgga ctacgccagc gccgacgcgc agccgcttgg cccgatgggg
     4861 ccatcgcgcc tgctgttgtc caaacccgtc atcgccgcca tcgaaggcgc ggcggtggcg
     4921 ggcggcatgg agctcgccct gtggtgcgac ttgcgcgtga tggccgagga cgcgtacttc
     4981 ggcatctact gccgccgctt tggcgtgccg ctgatcgacg gcggcacggt gaggctgccg
     5041 cgtctgatcg gccagagccg cgcgctggac ttgttcctga ccgggcgccg ggtcgatgcg
     5101 cccgaagcgc tggcgatggg cctggccaac cgcatggtcg gcgctggtca ggcgcttgat
     5161 gccgccgtcg cgctggcgcg gcagatcgcc gcctttccgc aagacacctt gcgcgccgac
     5221 cgcctgagcg cgctcacgca atgggactgg ccgctggccg aggccctgca ccgcgagtgg
     5281 acgcgcggca gccagcgcct ggccgacgcg ctgcagggcg cggcgcgctt tgccagtggc
     5341 gcgggccggc gtggcgagtt ttgatggcgg cagcggtgct tgtcgccccg cagacaggcg
     5401 ctggtctatt tcgccgatca aatgcttggt aaattcctat actgatccga ttcacctgcc
     5461 tggagccccg ccatgaccga agcctatgtg tttgacgcca tccgcacgcc gcgcggcaag
     5521 ggcaagaagg acggcagcct gcacgaggtc aagccggtcg atctgctggc cggcacgctg
     5581 acggcgctca aagaacgtca cgacttcgac accgccgccg tggacgacgt ggtgatgggc
     5641 gtggtctcgc cgattggcga gcagggctcg gtcatcgcca aggtcgcggc gctcaaggcc
     5701 ggctgggact ggcaatgcgc cggcgtgcag ctcaaccgct tctgcgcctc gggcctggag
     5761 gcggtgaaca tggcggcgca gaaggtgcgc tcgggctggg aagacctggt ggtggccggc
     5821 ggcgtggaga gcatgagccg cgtgccgatt ggcgcagacg gcggcgcctg ggcgcaagac
     5881 ccggcgacca acagcgcgac cgatttcgtg ccgcagggcg tgggggccga cctgattgcc
     5941 acgctggaag ccttttcgcg cgaggatgtg gaccgctttg ccgtcacctc gcagcagcgc
     6001 gcggccgcgg cgcgcgaggc ggggcgcttc aagggctccg tggtgcctgt caccgacttc
     6061 ctcggccaga ccatcctgga ggaagacgaa ttcatcaagc cgcgcaccac gatcgaggcg
     6121 ctggcgggcc tcaagccatc gttcgagcaa ctcggcgcga tgggtttcga cgcggtcgcc
     6181 atcagccgtt acccgcaggt cgagcgcatc acgcatgttc accacgccgg caactcgtcg
     6241 ggcatcgtcg atggcgcggc ggcggtgctg atcggcaacg aggcggccat caagacgcac
     6301 ggcttcaagc cgcgcgcgcg catcgtcgcc acggcgctgt cgggcgccga tccgaccatc
     6361 atgctcaccg gccccatgcc cgccacgcgc aaggcgctgg cgaaggcggg gctcaagatc
     6421 gaggacatcg acctgttcga agtgaatgag gcctttgccg ccgtgccgat gaagttcatg
     6481 aaggaattca acgtgccgca tgagaaggtc aacgtcaacg gcggcgccat cgccctgggc
     6541 catccgctgg gcgcgaccgg cgcgatcatt ctgggcacgc tgatcgacga actgcaccgc
     6601 cgccacttgc gctacggcct ggccacgctg tgcgtgggcg gcggcatggg gattgccacc
     6661 atcgtcgaaa gaatctgaat caaacaggcc tccagcgctt gatgggcaag cgaaagcagc
     6721 tatcaaattc atagaatcat gaaactcaag accttgcgct acgcgctgac ggacggcatc
     6781 gccagcatca ccttcgacga ggaaaactcg cccgtcaaca ccatgtgcct gcagtggcag
     6841 gacgatctgc agaccgtgac ggatcaggtg gtgaaggaca aggacgccat tcgcggcatc
     6901 gtgctggcct cggccaagaa gaccttcttt gctggtgctg acctcaaggg cgtgatgcgc
     6961 atgaccgggg ccgacgcgcc caaggtgttt gccgagatcg agcgcatcaa gaagaacttc
     7021 cgcacgctgg aaacgctggg caagccggtg gtgagctgcc tcaatggcgc ggcgctgggt
     7081 ggcggctggg aagtggcgct cgtcggccac catcgcattg ccgtcagcga cccgaagatc
     7141 cagttcggcc tgcccgaagt caccatcggc ctgatgcccg gcggcggtgg catcaccaag
     7201 atgacgcgcc tgcttggcct gatgggcgcg caaccctatg tgctggagag caagctgttc
     7261 aatcccattg aagcgcagca gatgggcgtg gtgcaccaac tggtcgacag cgcggacgag
     7321 ttgatcccgg ctgcccgcgc ctggatcgaa tcggtgcagg gcgacgcgga tgccgccacg
     7381 cacccgtggg accgcaagga ttacaagatg cccggcggca cgccga
//
LOCUS       DAOMVO010000104         7355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2663_length_7355_cov_18.677945, whole genome shotgun sequence.
ACCESSION   DAOMVO010000104 DAOMVO010000000
VERSION     DAOMVO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2663_length_7355_cov_18.677945"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="atpD"
                     /locus_tag="PKV30_06640"
     CDS             <1..1162
                     /gene="atpD"
                     /locus_tag="PKV30_06640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRL29929.1"
                     /translation="ATLGRIMDVLGNPIDERGPVSHEHLASIHRKAPSYDELSPSQEL
                     LETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMMELINNIAKAHSGLSVFAGVGERT
                     REGNDFYHEMAESGVVKLDDLPESKVAMVYGQMNEPPGNRLRVALTGLTIAESFRDEG
                     RDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGRLQERITSTKVGSITS
                     IQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIAALGIYPAVDPLDSTSRQLDPLVVG
                     EEHYNVARAVQGTLQRYKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFHVAE
                     VFTGSPGKYVSLKDTINGFKMIVGGECDAMPEQAFYMVGTIDEAMEKAKKMA"
     gene            1190..1609
                     /locus_tag="PKV30_06645"
     CDS             1190..1609
                     /locus_tag="PKV30_06645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRL29930.1"
                     /translation="MANTIHVDVVSAEESIFSGEATFVALPGEAGELGIYPRHTPLIT
                     RIKPGAVRIQKTDGGEEFVFVAGGILEVQPTVVTVLSDTAIRGKDLDEAKASEARKDA
                     EEALKNAKNEIDMARATSELAILAAQIAALRKYREKR"
     gene            1737..2336
                     /locus_tag="PKV30_06650"
     CDS             1737..2336
                     /locus_tag="PKV30_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRL29931.1"
                     /translation="MFSGKSRAGEKVQIKAAALLITEGALVELSAADAREVVSYMRPE
                     RIKAGEVVIREGESTRNDFMALVLDGEVTVENSVAAAHDSMVVSILGPGSLIGDMGII
                     DGGPRSATCTASTDLALAVLTREALTRLMDTHPSVAARLLLAMSKRIADHLRETNRKL
                     MTFAQVSKALQQELDAAHGVNKRLLDQLAAQAGKPTPAG"
     gene            complement(2346..2891)
                     /locus_tag="PKV30_06655"
     CDS             complement(2346..2891)
                     /locus_tag="PKV30_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRL29932.1"
                     /translation="MFQTLTRLIRHRWLDHTDAARAVPPQLAEQLRRRVHASEQRHSG
                     EVRICVEGGLPTSYLWRHLRDGVAMPDIVRQRALMMFSKLRVWDTSDNNGVLIYLLLA
                     EHAIELVADRGVARYVDPAVWQTVVAHLSDELRQGHFEQGLTQALEEVSAVLVAHFPD
                     SGADGVNTPGPNQLPDEPVLR"
     gene            complement(2896..3834)
                     /locus_tag="PKV30_06660"
     CDS             complement(2896..3834)
                     /locus_tag="PKV30_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRL29933.1"
                     /translation="MRPAHVLKALIAIFFIVIGAGTAHAQQLLPVPPLTGHVVDTSGT
                     LQPAEIAALDAKLAKLEQDKGSQVVVLMVPTTAPEDIAAFANRVGNTWKIGRKDVGDG
                     VIVLVAVKDRRMRIEVAKTLEGAIPDIAAKHIIDEAMAPHFRTGDYAGGLDAAIDQLG
                     ARIRGEALPPVTPPTLGRGDRGDGGFGFGIFEIAILLFILVPVISAVARGIFGRKLGA
                     LATGAGVGGVAFIVTASVVLAVVAGIVALLFALFSGMAAALPQARGRNGGWGGPVILP
                     PSGGGWGGGGGGFGGGGGFGSGGGGDFGGGGASGGW"
     gene            complement(3872..4498)
                     /locus_tag="PKV30_06665"
     CDS             complement(3872..4498)
                     /locus_tag="PKV30_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRL29934.1"
                     /translation="MKSWLIKIGAMLALAASLTGCGYNDFQRLDETTKSAWSEVVNQY
                     QRRADLIDNLVNTVKGEAKFEQDTLNQVIAARAKATSIQVTPETLNNPEAFKQFQAAQ
                     GELSSALSRLMVTVERYPDLKANKAFADLRVALEGTENRIAVARNRYIEAVKEYNVLA
                     RSFPTNLTGMIFGYKPKENFTVANEAAISNAPKVDFGVGPGPSPAASR"
     gene            4666..5112
                     /locus_tag="PKV30_06670"
     CDS             4666..5112
                     /locus_tag="PKV30_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRL29935.1"
                     /translation="MPKSRYRAAQIGGSADEVETAFYEALHNADLEQLMACWADEDEI
                     VCVHPGGPRLVGAAAIRQAFEQMFSVGAIHATPERLRRIEALGAAVHNVLERVDQQTA
                     EGMRRAWVVATNVYLKTAHGWRLVAHHASPGRSEVAEVTQHAQLLH"
     gene            5372..6361
                     /locus_tag="PKV30_06675"
     CDS             5372..6361
                     /locus_tag="PKV30_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL29936.1"
                     /translation="MAYRAPAWLPGGQLQTIWPALHARRVMGAPIDWRRERWDTPDGD
                     FVDVDWLQPSDNDEAARPLLVLFHGLEGSSRSHYAEAFAGWARTHGLRLAVPHFRGCS
                     GELNRAPRAYHSGDWAEVDWLLQRFHARQQASGGGALLAVGVSLGGNALLRWAGEAGH
                     TAAQRVGAVAAICAPLDLAAGGRAIGRGFNRQVYTRMFLRTMKTKAAAQLAQHPGLFD
                     AKRLGAARDLYAFDDVFTAPLHGFAGADDYYRRAAAKPLLADIRVPALALNALNDPFV
                     PADSLPRASEVGRHVTLWQPREGGHVGFAQGALPPGDVFWLPRAVGGWLLDRA"
     gene            6386..6928
                     /locus_tag="PKV30_06680"
     CDS             6386..6928
                     /locus_tag="PKV30_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HRL29937.1"
                     /translation="MDDIVKQAMAKWPNVPHAYGWLGLDARGNWYLRDAAAQALGGFA
                     SGVPGAKGALLQHEALMAFIQRNYLADEAGQWFFQNGPQRVFVELEAAPWIWRVAADG
                     AVSAHDGRPAEVREVFTDELGRVFMRTDRGFGLVHTQDVALVADAVEAGRWQPQPVAS
                     ADLPAREGYVLSPQAANGAP"
     gene            complement(7040..>7355)
                     /locus_tag="PKV30_06685"
     CDS             complement(7040..>7355)
                     /locus_tag="PKV30_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL29938.1"
                     /translation="SPAAAAAPAAVVVAAAPAAAAPAADPAVGKALYDKSCVTCHGAG
                     VAGAPKFGDKAAWAPFIATGMDAMLKVAINGKGAMPPRGASTATDDELRAAIQYMVDH
                     AK"
BASE COUNT         1212 a         2509 c         2471 g         1163 t
ORIGIN      
        1 cgccacgctg ggccgcatca tggacgtgct gggcaacccg atcgacgagc gcggtccggt
       61 cagtcacgag cacttggcct ccatccaccg caaggcgccc agctacgacg agctgtcgcc
      121 ctcgcaggag ctgctggaaa ccggcatcaa ggtgattgac ctgatctgcc cgttcgccaa
      181 gggcggcaag gtcggcctgt tcggcggcgc cggcgtcggc aagaccgtga acatgatgga
      241 actcatcaac aacatcgcca aggcgcactc cggtctgtcg gtgtttgccg gcgtgggcga
      301 gcgtacccgc gaaggcaacg acttctatca cgagatggcc gaatccggcg tcgtcaagct
      361 cgacgacctg cccgagtcca aggtggccat ggtctacggc cagatgaacg agccgccggg
      421 caaccgcctg cgcgtggcgc tgaccggcct gaccatcgcc gaatccttcc gtgacgaagg
      481 ccgcgacgtg ctgttcttcg tcgacaacat ctaccgctac acgctggccg gcaccgaagt
      541 gtccgcgctg ctgggccgca tgccttccgc cgtgggctac cagccgacgc tggccgagga
      601 aatgggccgc ctgcaggagc gcatcacctc caccaaggtc ggctccatca cgtcgatcca
      661 ggccgtctac gtgccggccg acgacctgac cgacccgtcg cccgccacca ccttcgccca
      721 cctggactcc accgtggtgc tgtcgcgtga cattgcggcg ctgggtattt acccggccgt
      781 ggacccgctg gactccacca gccgccagct cgatccgctg gtggtgggcg aggagcacta
      841 caacgtggcc cgcgccgtgc agggcacgct gcagcgctac aaggaactgc gcgacatcat
      901 tgcgattctg ggcatggacg aactgagccc cgaagacaag ctggccgtgg cccgcgcacg
      961 caagatccag cgtttcctgt cgcagccgtt ccacgtggcc gaggtgttca ccggctcgcc
     1021 cggcaagtac gtgtcgctga aagacaccat caatggcttc aagatgatcg tgggtggcga
     1081 gtgcgatgcc atgcccgagc aggccttcta catggtgggc accatcgacg aagcgatgga
     1141 aaaagccaag aagatggcct aaggcggcct cgaaccagga gcgccacaca tggccaacac
     1201 cattcacgtt gacgtggtca gcgccgaaga gtcgatcttc tcgggcgagg ccacgttcgt
     1261 ggccctgccc ggcgaagctg gcgagctggg catctacccg cgccacacgc cgttgatcac
     1321 gcgcatcaag ccgggcgccg tgcgcattca aaagaccgat ggcggcgaag aattcgtctt
     1381 cgtggccggc ggcattctgg aagtgcagcc caccgtcgtc accgtgctgt ccgacaccgc
     1441 gatccgcggc aaggacttgg acgaagccaa ggcgtccgaa gcgcgcaagg acgccgaaga
     1501 ggccttgaaa aacgccaaga acgagatcga catggcgcgc gccacgtcgg aactcgcgat
     1561 cctggcggcc cagatcgccg cgctgcgcaa gtaccgcgag aagcgttgaa tctcgcgcgg
     1621 tcgcgcggga cggcaaccgc tccactcacg tcatcacaga aggcccgccc atgcgggcct
     1681 ttgttttgac aactaatgcc ttcgttcgcg gacacggcgg tgcctaaaat catcggatgt
     1741 tttcaggcaa gtcacgggcg ggcgaaaagg tccagatcaa ggcggctgcg ctgctgatca
     1801 ccgagggtgc gctggtcgaa ttgagcgccg ccgacgcccg cgaagtggtc agctacatgc
     1861 ggcccgagcg gatcaaggct ggtgaggtgg tgatccgcga gggcgaatcc acgcgcaacg
     1921 acttcatggc gctggtgctc gatggcgagg tcacggtcga gaactcggtt gccgcggcgc
     1981 acgacagcat ggtggtgtcg atcctgggtc ccggcagcct gattggcgac atgggcatca
     2041 tcgacggcgg cccgcgctcg gccacctgca ccgcatcgac cgacctggcg ctggccgtgc
     2101 tcacgcgcga agcgctgacc cggttgatgg acacgcaccc cagcgtggcg gcacgcctgc
     2161 tgctggccat gtccaagcgc attgccgacc atctgcgtga aacaaaccgg aagctgatga
     2221 cgtttgcgca ggtcagcaag gcgctgcagc aagagttgga tgctgcgcat ggggtcaaca
     2281 agcggctgct cgatcaactg gccgcgcagg ccggcaaacc cacacccgct ggctgactgc
     2341 gcgggtcagc gcaacaccgg ctcgtcgggc aattgattcg gccccggtgt gttcacccca
     2401 tcggcgcccg agtccgggaa gtgcgccacc agcacggccg acacttcctc cagcgcctgc
     2461 gtcagccctt gctcgaagtg gccctggcgc aattcgtcgc tcaggtgcgc caccacggtc
     2521 tgccacacgg ccgggtcgac gtagcgcgcc acgcctcggt cggccaccag ctcaatcgcg
     2581 tgttcggcca gcagcagata gatcagaacg ccgttgttgt ccgatgtgtc ccagacgcgc
     2641 agcttggaaa acatcatcag cgcccgctgg cgcacgatgt ccggcatcgc cacgccgtcg
     2701 cgcaggtggc gccacaggta gctggtgggc aggccgcctt cgacgcagat gcgcacctcg
     2761 ccgctgtggc gctgttcgct ggcgtgcacg cggcggcgca actgttccgc cagctgcggc
     2821 ggcacggcgc gcgcggcatc ggtgtggtcg agccagcggt ggcgaatcaa gcgggtcaga
     2881 gtctggaaca tgcgctcacc acccgccgct ggcgccgccg ccaccaaaat cgccgccgcc
     2941 gccagagccg aagcccccgc cgccaccaaa tccaccgccg ccaccgcccc aaccgccgcc
     3001 agacggcggc aggatgaccg gcccgcccca accgccattg cgcccacgcg cctgcggcag
     3061 cgccgccgcc atgccgctga acagtgcaaa cagcagcgcc acgatgcccg ccaccacggc
     3121 cagcaccacg ctggcggtga cgatgaaggc cacgccgccg acgccggcgc cggtagccag
     3181 cgcacccagc ttgcggccga aaatgccgcg cgccacggcg ctgatgaccg gcaccaggat
     3241 gaacagcagg atagcgatct cgaaaatccc gaagccgaaa cctccatcgc cccggtcgcc
     3301 gcgccccagc gtgggcggcg tcaccggcgg cagcgcctcg ccgcggatgc gcgcgcccaa
     3361 ctgatcgatc gccgcatcca gcccgcccgc atagtcgccg gtgcgaaagt gcggcgccat
     3421 cgcctcgtcg atgatgtgct tggcggcgat gtcggggatg gcgccttcca gcgtcttggc
     3481 gacctcgatg cgcatgcggc ggtctttgac cgccaccagc acgatcacgc cgtcgccgac
     3541 gtccttgcgg ccgatcttcc acgtgttgcc gacgcggttg gcaaaggcgg cgatgtcttc
     3601 cggcgccgtg gtcggcacca tcaggaccac cacctgcgag cccttgtcct gctccagctt
     3661 cgccagcttg gcgtccagcg cggcgatctc ggccggctgc agcgtgcccg atgtgtcgac
     3721 cacgtgcccc gtcagcggcg gcacgggcag caactgctgc gcgtgcgccg tgcccgcacc
     3781 gataactata aagaagatag ctatcagcgc tttcaggacg tgcgcgggac gcaatttagg
     3841 cgatcaaatg ttcaggggcg agacgccgat ttcagcgcga tgcggcgggc gacggccccg
     3901 ggccaacgcc gaagtccacc ttgggcgcgt tcgaaatcgc cgcctcattc gccaccgtga
     3961 agttctcctt cggcttgtag ccgaagatca tgcccgtcag gttggtcggg aagctgcgcg
     4021 ccagcacgtt gtattccttc accgcctcga tgtagcggtt gcgcgccacg gcaatgcgat
     4081 tctcggtgcc ttccagcgcc acgcgcaggt cggcaaaggc cttgttggcc ttcaggtcgg
     4141 ggtagcgctc caccgtcacc atcaggcggc tcaacgcgct ggagagctca ccttgcgcgg
     4201 cctggaactg cttgaacgcc tccgggttgt tcagcgtctc gggcgtcacc tgaatgctgg
     4261 tggccttggc gcgggcggcg atcacctggt tgagcgtgtc ctgctcgaac ttggcctcgc
     4321 ccttgacggt gttgaccagg ttgtcgatca aatcggcgcg gcgctggtac tggttgacca
     4381 cttcgctcca tgcggatttg gtggtttcat ccaggcgctg aaagtcgttg tagccgcaac
     4441 cggtcagcga tgcggccagg gccagcatgg cgccgatctt gatgagccag cttttcatgg
     4501 ggttcctcca gagagagttg atccgaacag gcgcggaagt tagcacacct gccgatgccg
     4561 atctgtaggc cgagcgcccg tggcgaggca gcggcccgcg ttgaggtggc agcgcatggc
     4621 gtgggcacgg cacaatcagg ccacgcctgc aaccgacgaa tcgccatgcc caagtcccgc
     4681 taccgcgccg cccagattgg cggctccgcc gacgaggtcg agacggcgtt ctacgaggcg
     4741 ctgcacaacg ccgacctgga acagctcatg gcctgctggg ccgacgagga cgagatcgtc
     4801 tgcgtgcacc cgggcggccc gcggctggtc ggcgcggcgg ccatccggca ggcgttcgag
     4861 cagatgttca gcgtcggcgc catccacgcc acgcccgagc ggctgcgccg tatcgaagcg
     4921 ctgggcgcgg ccgtgcacaa cgtgctggag cgcgtcgatc agcaaaccgc cgaaggcatg
     4981 cgccgcgcct gggtggtggc caccaacgtg tacctgaaaa ccgcgcacgg ctggcgcctg
     5041 gtggcgcacc acgccagccc ggggcgcagc gaagtggccg aggtgacgca gcacgcgcag
     5101 ctgctgcatt gaagttggcg gcatgcgcgg cgcatgaaga ttccagtcaa aaacggcccc
     5161 ggcgcttgcc tggcaagcgt gagcagctat ggtttttgca aatgaccgcg cctgcggcgc
     5221 atgaccgtgg ctggcgcctt cgatgacggc gctgcgcgcc atgacgggaa gtctggtcat
     5281 tcgctcatcg aagcgcagcg acgtcatttc gtcatcaaaa atggccccag tgcttgactg
     5341 acaggcgcga gcagctattg tttttgaagt gattgcctac cgcgcccccg cctggctgcc
     5401 cggcggccag ctgcaaacca tctggccggc gctgcacgcg cggcgcgtga tgggcgcgcc
     5461 catcgactgg cggcgcgagc gctgggacac gcccgacggc gactttgtcg atgtggattg
     5521 gctgcagccg tctgacaacg atgaggcggc caggccgctg ctggtgttgt tccacggcct
     5581 ggaaggctcg tcgcgcagcc actacgccga ggcgtttgcc ggttgggcgc gcacgcacgg
     5641 gctgcgtctt gccgtgccgc actttcgcgg ctgctcgggc gagctgaacc gcgcgccgcg
     5701 tgcctaccac tcgggcgact gggccgaggt cgactggctg ctgcagcgct ttcacgcccg
     5761 tcagcaggcc agcggtggcg gcgcgctgct ggcggtcggc gtatcactgg gcggcaatgc
     5821 gctgctgcgc tgggccggcg aggcgggtca caccgcagcg cagcgcgttg gcgccgtggc
     5881 ggcgatttgc gcgccgctcg atctggcggc cggcgggcgc gccatcgggc gcggcttcaa
     5941 ccggcaggtc tacacccgca tgtttttgcg caccatgaaa accaaggccg ccgcccaact
     6001 ggcgcagcac ccgggcctgt tcgacgccaa gcgcctgggt gccgcgcgtg atttgtacgc
     6061 cttcgacgat gtgttcaccg cgccgctgca cggctttgcc ggcgccgacg actactaccg
     6121 ccgcgccgcc gccaagccgc tgctggccga catccgcgtt cccgcactgg cgctgaacgc
     6181 gctgaacgac cccttcgtgc ccgccgactc gctgccgcgc gccagcgagg tcggccgcca
     6241 cgtcacgctg tggcagccgc gcgagggcgg tcacgtcggc tttgcccaag gtgcgctgcc
     6301 gccgggcgac gtgttctggt tgccgcgcgc agtgggcggc tggctgcttg accgcgcatg
     6361 acgaacgcac gcccataatg gcccgatgga cgacatcgtc aagcaggcca tggccaaatg
     6421 gcccaacgtg ccgcacgcct acggctggct ggggctggac gcgcgcggca actggtatct
     6481 gcgcgacgcc gccgcgcagg cgctgggcgg ttttgccagc ggcgtgccgg gcgccaaggg
     6541 tgcgctgctg cagcacgagg cgctgatggc gttcatccag cgcaactatc tggccgacga
     6601 ggccgggcaa tggtttttcc agaacgggcc gcagcgcgtg tttgtcgaac tcgaggccgc
     6661 gccctggatc tggcgcgtgg cggccgatgg tgcggtgagc gcgcacgatg ggcggccggc
     6721 cgaggtgcgc gaggtgttca ccgacgagct gggtcgcgtc ttcatgcgca ccgacagggg
     6781 ctttggcctg gtgcacacgc aggacgtggc gctggtcgcc gacgccgtgg aagccggccg
     6841 ttggcaaccg cagccggtgg cgagcgccga tctgccggcg cgcgagggct acgtgctgag
     6901 tccgcaagcc gccaacgggg caccttgatg actcatcatt tgatagctgc tcgcgcttga
     6961 tggacatgcg ccggcgcctg attcgacatg aaaaaagccg ctgacacagc ggcttttttt
     7021 gtgggcgagg cagcccggct tacttggcgt gatcgaccat gtactggatg gccgcgcgca
     7081 gctcgtcgtc ggtggcggtc gaggcgccgc gcgggggcat ggcgcccttg ccgttgatgg
     7141 cgactttcag catggcgtcc atgccggtgg cgatgaaggg tgcccacgcg gccttgtcgc
     7201 cgaacttggg cgcgccggcc acacccgcgc cgtggcaggt aacgcacgac ttgtcgtaca
     7261 gcgccttgcc gaccgccggg tcggccgcgg gcgcggctgc agccggtgca gcagccacca
     7321 cgaccgcggc cggcgcggcc gctgcagcgg gcgac
//
LOCUS       DAOMVO010000105         7300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2698_length_7300_cov_16.345618, whole genome shotgun sequence.
ACCESSION   DAOMVO010000105 DAOMVO010000000
VERSION     DAOMVO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2698_length_7300_cov_16.345618"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PKV30_06690"
     CDS             <1..1019
                     /locus_tag="PKV30_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRL29939.1"
                     /translation="AEHGGALPQLDSRALVVLRETLAFYSPLADFAFAMQGLGSGAIS
                     LGGTPAQQAAYLPAVARGDKIAAFALSEPDAGSDVAAMKTRAACENQTSAGGQNHSES
                     TDWKLTGTKTWISNGGLADFYCVFAKTDRLAGSRGITAFIVDKGAPGLDDSAHIHVMA
                     PHPLATLKLTDCAVSGDMPLGEVNGGFKLAMQTLDIFRASVAGAALGMARRALAEAID
                     YARRRQMFGQTLADFQLTQAKLGEMAALVDAGALLTYRAAWMRDTQQGSLKERSVAAA
                     MAKMTATENAQRVIDMALQMHGGRGVEVGAKVESLYRDIRSLRIYEGATEVQQLIIGK
                     AVLS"
     gene            1016..2638
                     /locus_tag="PKV30_06695"
     CDS             1016..2638
                     /locus_tag="PKV30_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL29940.1"
                     /translation="MTTPSAQTDRFVHDRLPPRDQWPELRYDLPELRIADQANLVERL
                     LDGAAARGWADRPLLRSPQITFTYAETRERVDRIANYLVHELKLEPGNRVLLRGGNSI
                     GMALSWLAVVKAGLIAVATMPLLRATELSKVIDKAQPVAAICDARLLQELEQAQQAFP
                     ALQHVLRFNSPDDPSDLGQLVAHQAATFTACPTSTDDIALLAFTSGTTGAPKAVVHTH
                     RDVLAACEAWPRHVLRPTSDDIVTGSPPLAFTFGLGGLLLFPMWAGASIYYPDANYNP
                     ETMVRQMSEQGVTTCYTSPTFYRQLAPHMKAIGVPSLRTCVSAGEALPDATRQLWKQA
                     TGIEMLDGIGATEMFHIFISSAGADVRRGAVGKVVPGYHAKVVDEQGAEVPRGTVGKL
                     AVIGPTGCRYMDDERQRRYVPDGWNRPGDAFVQDEDDYFHYQARDDDMIITSGYNVGG
                     PEVEDCLLTHPAVAECGVIGKPDDERGMIIKAVVVLKPGHAGDAAMVKTLQDHVKASI
                     APYKYPREVAFVTTLPRTETGKLQRFRLRQTP"
     gene            2635..3033
                     /locus_tag="PKV30_06700"
     CDS             2635..3033
                     /locus_tag="PKV30_06700"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL29941.1"
                     /translation="MTHTFLQPPGWAPAKGYANGVAARGTLVFVGGQIGWNAQQQFET
                     DDFIEQTRQTLQNVVAVLKEAGAGPEHMVRMTWYVIDRVEYNARLKELGGVYREVIGK
                     HFPAMTCVEVAALMEARAKVEIEVTAVLPD"
     gene            3089..4240
                     /locus_tag="PKV30_06705"
     CDS             3089..4240
                     /locus_tag="PKV30_06705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRL29942.1"
                     /translation="MIATFSASPHRASAERLVFLDWLRIYAFASVLAGHKFHEPVALA
                     AQAETAWLRWPARLLWPFIEGGGVGVVVFFLISGYIITQVLQRESAPEFLLKRAFRIY
                     PLYLVAVGIEFLILRSHGQGVGWQTLLQQMLLVGDFFGTPYALAGVEWTLRLEILFYL
                     LMAGLRTLGLTRMDRAGPLAITYVLLIAALHFCGPLASHTAWTRGYLSLFMPFLLLGS
                     MLWLYEKDAVNRSTMLAFILLTLVAYRVGLHAWQPRWLDAYFGVLGIAVFTCCWAVRQ
                     WLPAPAWLLALSELTYAVYLLHNWLFDVFLQAFQRLGLRDVYAALATLALLGAVCWLL
                     VRCVERPGVRWGRTLASRMSSQAPALTPPEHHGRVAASSRTPPPASSRS"
     gene            complement(4167..5627)
                     /locus_tag="PKV30_06710"
     CDS             complement(4167..5627)
                     /locus_tag="PKV30_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL29943.1"
                     /translation="MLQRAATETLTEQLAGRFAARIRDRLLAPGTRLPSVRQCAAQHQ
                     VSPSTVVAAYDQLLAGGLVEARRNRGFYVRDWARKVPPALAESAQAATETRAAVDPTE
                     ARRLSAPISAATLMRGMFGADGKPQPGMGVLPPDWLDNDFMAAALRRAASGSGLRDSS
                     VRYGDPRGDPQLRQVLAARLQAQGIAATPAQIVTTVGATQALDIVSRTLLKPGDTVMV
                     EEPGWSVEFARLSALGMRLLPVPRGPDGPDLAVMARYCEAHAGTAQAPRLFVSVSVLH
                     NPTGYSLSAASAHRMLQLAQQRDFHIVEDDTYHHLAPEHATRLAQLDGLARTIYVSGF
                     AKILAPAWRVGFLAAPPDLVEPLMDTKLLATLTTPSLLERALAQCIEQGHLRRHAERV
                     RAGLDAARIRSVRLAQSAGCTFMSEPAGMFGWIDTGVDTEPLSLRMLDEGYLLAPGAL
                     FHAQRAPSTLMRINFATTQEAAFWTKLQRARDALAA"
     gene            5707..6315
                     /locus_tag="PKV30_06715"
     CDS             5707..6315
                     /locus_tag="PKV30_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL29944.1"
                     /translation="MPWSEFTALLLLATAVSFTPGPNTTLSTAIAANRGLRGALRFVC
                     AVPVGWSALLVLCALGVGALLLAVPALRWAVLLAGVAYMLWLASRLAGSATLAQADAG
                     RLDVGFWQGVLLQFVNIKAWMLALSIVAGWLAGRPDWLARFALVLPVMVAYAFFSNLL
                     YAAIGSLLRGWLAHGRRLLWFNRVMALALVATAAWMLWSAST"
     gene            6453..>7300
                     /locus_tag="PKV30_06720"
     CDS             6453..>7300
                     /locus_tag="PKV30_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL29945.1"
                     /translation="MLFGFWLGVLGVTCFAITLPATRLATGTAADPQLSPAFVTAGRA
                     ALAGLLSAAFLLIARSPWPTGAQWRALSLSMAGNVIGFPLLLAWALRSVTATHAAVIL
                     ALLPLATAAVAAWALHQRARPAFWACAVLGSALVVVFAWMRTGAHGGFGLTSADWLLA
                     GAVVAASVGYVYGAKVTPALGAERVICWVCLGALPITLPIALWLWPANAGDIRPSAWA
                     GFAYVGTVSMWAGFFAWYRGLDWGGALRVSQTQLLQPFLAMLFAWPLLGERLDAVSIG
                     FALAVVA"
BASE COUNT         1009 a         2417 c         2586 g         1288 t
ORIGIN      
        1 cggccgagca cggcggtgcg ttgccgcaac tcgactcacg cgcgctggtg gtgctgcgcg
       61 agacgctggc gttttattcg ccgctggccg actttgcgtt tgccatgcaa ggcctgggca
      121 gcggcgcgat ttcgctcggc ggcacgccgg cgcagcaagc ggcgtatctg cccgcggtgg
      181 cgcgcggcga caagatcgcg gcctttgcac tgagtgagcc ggatgcgggc tcggatgtcg
      241 cagctatgaa aacaagagct gcttgcgaaa atcagaccag cgctggcggc caaaatcatt
      301 ctgaatcgac cgattggaaa ctcaccggca ccaagacctg gatcagcaac ggcggcctgg
      361 ccgatttcta ttgcgtgttc gccaagaccg accgcctggc cggcagccgc ggcatcaccg
      421 ccttcatcgt cgacaagggc gcgccgggcc tggacgacag cgcgcacatc cacgtgatgg
      481 cgccgcatcc gctggccacg ctgaagctga ccgactgcgc cgtgtctggc gacatgccgc
      541 tgggcgaggt gaacggtggc ttcaagttgg cgatgcaaac gctcgacatc ttccgcgcca
      601 gcgtggccgg cgctgcgctc ggcatggcgc gccgcgcgct ggccgaagcc atcgactacg
      661 cgcgccgccg ccagatgttc ggccagacgc tggcggactt ccagctcacg caagccaagc
      721 tgggcgagat ggcggcgctg gtcgacgccg gcgcgctgct gacctaccgc gccgcgtgga
      781 tgcgcgacac gcagcaaggc agcttgaaag agcgcagcgt cgctgcggcg atggccaaga
      841 tgacggcgac cgaaaacgcg cagcgcgtga tcgacatggc gctgcagatg catggcgggc
      901 gcggtgtcga ggttggtgcc aaggtcgaat ctctctaccg cgacatccgc agcctgcgca
      961 tctacgaagg cgcgacggaa gtgcaacaac tcatcatcgg aaaggctgtg ctttcatgac
     1021 aacgcccagc gcccagaccg accgcttcgt ccacgaccgt ctgccgccgc gcgatcagtg
     1081 gcccgagttg cgttacgacc tgccggagtt gcgcatcgcc gaccaggcca acctggtcga
     1141 gcgcctgctc gatggcgccg cggcgcgcgg ttgggccgac cggcccttgc tgcgctcgcc
     1201 gcagatcacc ttcacctacg ccgaaacgcg cgagcgggtc gatcgcatcg ccaactacct
     1261 tgtccacgag ctgaagctgg agcccggcaa ccgcgtgctg ctgcgcggcg gcaactcgat
     1321 tggcatggcg ctgtcgtggc tggcggtggt caaggccggg ctgatcgcgg tggccaccat
     1381 gccgctgctg cgcgccaccg agttgagcaa ggtgatcgac aaggcgcagc cggtggccgc
     1441 catttgcgac gctcgcttgc ttcaagaact ggagcaggca cagcaggcat ttccggcgct
     1501 gcagcatgtg ttgcgcttca actcgccgga cgatccgagc gatcttggtc agctggtggc
     1561 gcatcaggca gccacgttca cggcctgccc gacttccacc gacgacatcg ccctgctcgc
     1621 cttcaccagc ggcaccaccg gcgcgcccaa ggccgtggtg cacacgcacc gcgacgtgct
     1681 ggcggcctgc gaggcctggc cgcgccacgt gctcaggccg acatcggacg acatcgtcac
     1741 cggttccccg ccgctcgcct tcaccttcgg gctgggtgga ttgctgctgt ttccgatgtg
     1801 ggctggcgcg tcgatctact accccgacgc caactacaac cccgagacca tggtgcgcca
     1861 gatgagcgag cagggcgtca ccacctgcta cacctcgccc actttctatc gtcagctggc
     1921 gccgcacatg aaagccatcg gcgtgcccag cctgcgcacc tgcgtcagcg cgggcgaggc
     1981 gctgcccgac gccacgcgcc agttgtggaa gcaggccacg ggcatcgaga tgctggatgg
     2041 catcggcgcc accgagatgt tccacatctt catctcctcg gccggcgcgg atgtgcggcg
     2101 cggcgcggtg ggcaaggtgg tgccgggtta ccacgccaag gtggtggatg agcagggcgc
     2161 cgaggtgccg cgcggcacgg tgggcaagct ggccgtcatc ggccccaccg gttgccgcta
     2221 catggacgac gagcgccagc gccgctatgt gcccgatggc tggaaccgcc ccggcgacgc
     2281 cttcgtgcag gacgaggacg attacttcca ctaccaggcg cgcgacgacg acatgatcat
     2341 cacatcgggc tacaacgtgg gcgggccgga ggtggaagac tgcctgctga cgcatccggc
     2401 ggtggccgag tgcggcgtga tcggcaagcc cgacgacgag cgcggcatga tcatcaaggc
     2461 cgtggtggtg ctgaagcccg gacacgcggg cgacgcggcc atggtcaaga cgctgcaaga
     2521 ccacgtcaaa gccagcatcg cgccctacaa atacccgcgc gaagtggcat tcgtcaccac
     2581 gctgccgcgc accgagaccg gcaagctgca gcgctttcgt ttgaggcaaa ccccatgacg
     2641 cacacctttt tgcaaccgcc cggctgggcg cccgccaagg gctatgccaa cggcgtggcc
     2701 gcgcgcggca cgctggtgtt tgtcggcggc cagatcggct ggaacgccca gcagcagttc
     2761 gagaccgacg atttcatcga acaaacgcgc cagacgctgc aaaacgtcgt tgcggtgctg
     2821 aaagaagcgg gcgccggccc cgagcacatg gtgcgcatga cctggtacgt gatcgaccgt
     2881 gtggagtaca acgcgcgcct gaaagaactg ggcggcgtat accgcgaggt gatcggcaag
     2941 cacttccccg ccatgacctg cgtcgaggtg gcggcgctga tggaagcgcg cgccaaggtg
     3001 gagatcgagg tgacggccgt gctgccggac tgacggcggg cagcacgttc gggaccgagc
     3061 cgttctgagg ccggataatc ggcacccaat gattgcgacg ttttcggcca gccctcaccg
     3121 agcttccgcc gaacggttgg tctttctcga ctggctgcgc atttacgctt tcgccagcgt
     3181 gctggcgggc cacaagtttc atgaacccgt ggcgctggcg gcgcaggccg agaccgcgtg
     3241 gctgcgctgg cctgcgcgcc tgctctggcc gttcatcgag ggcggcggcg tgggggtggt
     3301 ggtgttcttc ctgatctccg gttacatcat cacgcaggtg ttgcagcgcg agagtgcgcc
     3361 cgagtttctg ctcaagcgcg cgtttcgcat ctacccgctc tatctggtgg cggttggcat
     3421 cgagttcttg atcctgcgtt cccacggcca aggtgtcggc tggcagacac tgctgcagca
     3481 aatgttgctg gtgggcgact tcttcggcac gccgtatgcg ctggccggcg tcgaatggac
     3541 gttgcgactc gagatcctgt tttatctgct gatggcaggt ttgcgcacgc ttgggctgac
     3601 gcggatggat cgcgcagggc ccctggcgat cacgtatgtg ctgttgatcg cggcccttca
     3661 tttctgcggg ccactggcct cgcacaccgc ctggacgcgg ggctacctgt cgctgttcat
     3721 gcctttcctg ttgcttggct cgatgctgtg gctgtacgaa aaagacgcgg taaaccggtc
     3781 aaccatgctg gccttcatcc tgttgacgct ggtggcttac cgtgtgggcc tgcatgcttg
     3841 gcagccgcgg tggctggatg cctatttcgg cgtgctgggc atcgcggtgt tcacttgttg
     3901 ctgggccgtg cgtcaatggc tgccggcgcc tgcctggctg ctggcgctgt cggagttgac
     3961 ctatgcggtc tacttgctgc acaactggct gttcgacgtc tttctgcagg ccttccaacg
     4021 gcttggattg cgtgacgtgt acgccgccct ggccacgctg gcactgctgg gggccgtgtg
     4081 ttggcttctc gtgcgttgcg tcgagcggcc aggcgtcagg tggggacgca cgctggcgtc
     4141 acgcatgagc agccaggcgc cggccctcac gccgccagag catcacgggc gcgttgcagc
     4201 ttcgtccaga acgccgcctc ctgcgtcgtc gcgaagttga tgcgcatcag cgtgctgggc
     4261 gcgcgctggg cgtggaacag cgcgccgggc gccagcaggt agccttcatc cagcatgcgc
     4321 agcgacagtg gttcggtgtc gacgccggtg tcgatccagc cgaacatgcc ggccggctct
     4381 gacatgaacg tgcagcccgc cgattgcgcc agccgcacgc tgcggatgcg cgcggcgtcc
     4441 agcccggcgc gcacgcgctc ggcgtggcgg cgcaggtggc cttgctcgat gcattgcgcc
     4501 agcgcgcgct ccagcaggct gggcgtggtc agcgtggcca gcagcttggt gtccatcagc
     4561 ggctcgacca ggtcgggcgg cgccgccaga aagcccacgc gccaggccgg cgccagaatc
     4621 ttggcaaagc cgctgacgta gatggtgcgc gccagcccgt cgagctgcgc cagccgcgtg
     4681 gcgtgctcgg gcgccaggtg gtggtaggtg tcgtcctcga cgatgtgaaa gtcgcgctgc
     4741 tgcgccagtt gcagcatgcg gtgcgcgctg gcggccgaca ggctgtagcc cgtggggttg
     4801 tgcagcacgc tgacgctgac gaacaggcgc ggcgcttgtg ccgtgccggc atgcgcctcg
     4861 cagtagcggg ccatcacggc caagtcgggc ccgtccggcc cgcgcggcac gggcagcagg
     4921 cgcatcccca aggccgacag gcgcgcgaat tcgaccgacc agccgggctc ttcgaccatc
     4981 accgtgtcgc cgggtttgag cagcgtgcgg ctgacgatgt cgagcgcctg tgtggcgccc
     5041 acggtggtga cgatttgcgc cggtgtggcg gcgatgccct gcgcctgcag ccgcgctgcc
     5101 agcacctggc gcagttgcgg gtcgccgcgc gggtcgccat agcgcacgct gctgtcgcgc
     5161 aggccgctgc cgctggcggc gcggcgcaag gcggcggcca tgaagtcgtt gtcgagccag
     5221 tcgggcggca gcacgcccat gccgggctgc ggcttgccgt cggcgccgaa catgccgcgc
     5281 atcagcgtgg ccgcgctgat cggcgcgctc agccggcgcg cctcggtggg gtcgacggct
     5341 gctcttgttt cagtagctgc ttgcgccgat tctgcaagcg ctggcggcac ttttcgtgcc
     5401 caatcgcgga cgtagaagcc gcggttgcgc cgcgcctcca ccaggccgcc agccagcaac
     5461 tggtcgtacg ccgccaccac ggtggacggg ctgacctggt gctgcgccgc gcactggcgc
     5521 acgctgggca ggcgcgtgcc gggcgccagc aaacggtcgc ggatgcgggc ggcaaagcgg
     5581 ccggccagtt gctcggtcag ggtttcggtg gcggcgcgtt gcagcatggc cgcgaccata
     5641 cagtttgtga tctgcgcttg tcaactgtat tgatggtgtg tggtctgaca acgctagagt
     5701 ggcgccatgc cctggtccga attcactgcc ctgctgttgc tggcgactgc cgtcagcttc
     5761 acgccggggc ccaacaccac gctgtcgacc gcgattgccg ccaaccgcgg cttgcgcggc
     5821 gcgctgcgat ttgtgtgcgc ggtgccggtg ggctggtcgg cgctgctggt gctgtgcgcg
     5881 ctgggggtcg gcgccttgct gctggcagta ccggcgctgc gctgggcggt gctgctggcc
     5941 ggcgtggcct acatgctgtg gctggcctcg cgcctggccg gcagcgccac gctggcgcag
     6001 gccgatgcgg gccggctcga cgtcggcttt tggcaaggcg tgctgctgca gttcgtcaac
     6061 atcaaggcgt ggatgctggc gctgtccatc gtcgccggct ggctggccgg gcggcccgat
     6121 tggctggcgc gcttcgcgct ggtgctgccg gtgatggtgg cgtatgcgtt tttcagcaac
     6181 ctgttgtatg ccgccatcgg ttcgctgctg cgcggctggc tggcgcatgg gcggcgcctg
     6241 ctgtggttca accgcgtgat ggcgctggcc ttggtggcca cggccgcgtg gatgctttgg
     6301 agtgcgagca catgatccca cctgagtcgc ctgcggcgcc ttccccccag ggggacgccg
     6361 ccagtggccg ggcagagccc gttccacggc ggcacgcgca tggcctgctc cgcggccttc
     6421 tgaattttca ggcgccaacg ggcgatgcgc gcttgctgtt cggcttctgg ctcggcgtgc
     6481 tgggcgtgac ctgctttgcc atcacgctgc cggccacgcg gctggcgacg ggcacggcgg
     6541 ccgatcccca actgtcgccg gccttcgtca ccgctggccg cgccgcgttg gcggggctgt
     6601 tgtcggcggc cttcctgctc atcgcgcgct cgccctggcc gaccggcgcg cagtggcgcg
     6661 cgctgtcgct gtcgatggcc ggcaacgtca tcggctttcc gctgctgctg gcctgggcgc
     6721 tgcgcagcgt gacggccacg cacgcggcgg tgattctggc gctgctgccg ctggccacgg
     6781 ccgccgtcgc ggcctgggcg ctgcaccagc gcgcgcggcc ggcgttctgg gcctgcgccg
     6841 tgctcggcag cgcgctggtg gtggtgttcg cgtggatgcg caccggcgcg catggcggat
     6901 ttggcctgac ctcggccgac tggctgctgg ccggcgccgt cgtcgcggcg tcggtcggct
     6961 atgtgtacgg cgccaaggtc acgcccgcgc tcggcgccga gcgggtgatc tgctgggtct
     7021 gcctgggcgc gctgcccatc acgctgccca tcgcgctgtg gctgtggccg gccaatgcgg
     7081 gcgacattcg ccccagcgcc tgggccggct tcgcatacgt cggcacggtg tcgatgtggg
     7141 ccggcttctt cgcctggtac cgcgggctgg actggggcgg cgcgctgcgc gtgagccaga
     7201 cgcagctgct gcaacccttt ttggccatgc tgttcgcctg gccgctgctg ggcgagcggc
     7261 tggatgcggt gtcgatcggc tttgcgctgg cggtggtggc
//
LOCUS       DAOMVO010000106         7231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2735_length_7231_cov_15.802397, whole genome shotgun sequence.
ACCESSION   DAOMVO010000106 DAOMVO010000000
VERSION     DAOMVO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_7231_cov_15.802397"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1200)
                     /locus_tag="PKV30_06725"
     CDS             complement(40..1200)
                     /locus_tag="PKV30_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRL29946.1"
                     /translation="MKPSLNWKLSALAVAVALSACGGGDDDKNPLGINAVKVIGDSLS
                     DGGTFSGIPGGARIASVQGSSDEPNVLWVERVAKAYELAPLCPVYKFNGTTFAANTQA
                     GCTNFAIGGARINNPAASGGAAAPLSIGKQLTDAAAKGWGAKDLVLVDGGGNDAADVV
                     GAYLGAAADQGAAYQALLLTLIPATTLQTVLAGANGPENAGGLYMQALADNFAGTIKA
                     QALDKGAKQVVVANIPTITYTPRFQAVLDQIGASAAGPAGRAQAEALFKGWVNAFNQR
                     LAANFSGESRVKVVDLATRFTDQVLNPAKYNLTNVTLPVCGAAWVTVVPQRSFAECTA
                     SALSATTPPPGAPSGSGWWQRYLFADGFHPTPYGHQLFANQVLDLLDDADWH"
     gene            1299..2372
                     /locus_tag="PKV30_06730"
     CDS             1299..2372
                     /locus_tag="PKV30_06730"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRL29947.1"
                     /translation="MNTAPRTHRIAVIPGDGIGQEVMPEALRALRAAEGKFDFKLDLT
                     PITWASCDHYAKTGQMMPDDWKAQLETQDAILFGAVGWPATVPDHISLWGSLLKFRRE
                     FDQYINLRPVRLFEGVPCPLAGRKPGEIDFLVVRENTEGEYTNLGGVMYAGTEREIVV
                     QETVMSRFGADRVLKFAFEQAAARPRQKLDVATKSNGIAISMPWWDGRADAVHQSYPH
                     IAVEKHHIDILTARFVLQPQRFDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPER
                     KFPSLFEPVHGSAPDIFGQGIANPVAMIWSAAMMLQFLGEQAAHDAMLKAIEQVLAKG
                     PRTPDLGGKASTTEMGEALAALI"
     gene            complement(2559..4025)
                     /gene="dacB"
                     /locus_tag="PKV30_06735"
     CDS             complement(2559..4025)
                     /gene="dacB"
                     /locus_tag="PKV30_06735"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRL29948.1"
                     /translation="MTASSAWSQPTLPPEIAQALAKAGVPTSAVAMVVAPLPPPPGTV
                     TRVPEPVNPSGERNAAPAAASMALPPPRLSWQPDVPMNPASVMKLVTTYAGVDQLGPS
                     HFWRTRVYTQGYIKDGVLNGNLLIQGSGDPKLVVERLQELMRAIQDKGVRQINGDILL
                     DNTIFRLPAHDAAAFDDDPLRPYNVGPDGLLLNFKALVFKFIPDPANHRVLVQSEPPM
                     AGVAIPTEVPAVGGACGNWRGRLAADFTDPNRVSFSGRYPTSCGEQNWSIAYVEPASY
                     APRVIEAMWRGAGGALNGQVKWSDRPATGQPLVTGFSLPLADIIADINKFSNNVMAQQ
                     LFLTLSARDGRASLAESQNTLRRWWRDRFGLRTTPIVENGSGLSRNERITAASLAALL
                     QQAAGHPNGAVFEHSLSIAGVDGTTRRMAARSPSSAAIGNARLKTGTLRDVTAIAGYA
                     YGLSGRNYAVVGIINHPNAGAARPALDRLVEWAVRDAP"
     gene            4216..5214
                     /locus_tag="PKV30_06740"
     CDS             4216..5214
                     /locus_tag="PKV30_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL29949.1"
                     /translation="MRLSPRATGIAAAVITVAIWTGFIVIGRASAGRTLLPFDIGLLR
                     VLGASAVLLPWGWWLTRGRRDTASSSLLGLSPLPLRQTVLAGFFGGFIYAVLCYAGFF
                     YAPAAHASVLMPGSLPLWTALLAALVLRDRITPARALGLACIVVGDLVVGGASLLQAF
                     NGGSVWKGDVIFMSAAFCWSVYSVLARKFGLEAVRATIAITAFAFFTYVPLFTLLTAS
                     GVLPTHLASAPWGEIAFQALFQGVGSVVISGITFVQMVRAFGPVKSTMITALVPGLSA
                     LGAVLLLGEPLGWNLLAGLALVTAGILFGVRAARQTTTDLIAARACPASAGAPKQS"
     gene            5226..5924
                     /gene="tsaB"
                     /locus_tag="PKV30_06745"
     CDS             5226..5924
                     /gene="tsaB"
                     /locus_tag="PKV30_06745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRL29950.1"
                     /translation="MNLLALDTSTDQLSVAVQRADGQRFAHTGAGGAQASASLIPAVQ
                     ALLAQAGLSLSDLSAIAFGRGPGSFTGLRTACSVAQGLAWGAGVPVLPIDTLLAVAEA
                     ARSAHGATHVLALLDARMNEVYAAEYAWRDAAGWRPLSNIAVLAPEAVPTPGAGAVLA
                     GNAFAAYADRLPAALQALPRIAALPSADALLNLAPALLAAGAAVPAEQALPLYIRDKV
                     AQTTAERSAAGLAR"
     gene            5938..6432
                     /gene="rimI"
                     /locus_tag="PKV30_06750"
     CDS             5938..6432
                     /gene="rimI"
                     /locus_tag="PKV30_06750"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRL29951.1"
                     /translation="MSAVLKPAPSSAVEAHLEPMTPERLPAVLAVEQSVYSHPWTRGN
                     FTDTLAAGHHALCLLGGDELIGYFVAMRGFEEVHLLNITVAPAHQRQGWALVLLDALV
                     LWSRAQGAQWLWLEVRASNTRAQQIYLKRGFARVGLRKAYYPAGHGLREDAVVMNLRL
                     ADGA"
     gene            6429..>7231
                     /locus_tag="PKV30_06755"
     CDS             6429..>7231
                     /locus_tag="PKV30_06755"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRL29952.1"
                     /translation="MSLALDERQRAMLAEMGIKLWAPSAPRVAEVAPEKEAARAGETS
                     AGAEFDSKPTPTASLQTAAEAPIVRPPVRAIAAPLPTAPAAPVPTALGPRPEGVADMD
                     WPQLEATVAACRACGLCAGRTQTVFGVGSRTADWMVIGEAPGENEDRQGEPFVGQAGK
                     LLDNMLAAIGLSRQVGDTMNSVAAGGDSVRGDGPKDSKQGVYIANVLKCRPPGNRNPQ
                     PDEITQCEPYLRRQVALVQPKIILAMGRFAVQSLLQTTEPIGKLRGRVH"
BASE COUNT         1085 a         2478 c         2520 g         1148 t
ORIGIN      
        1 ggcccgcggg ccgctttttt tatggcgcgc gcgcaggcgt caatgccagt cggcgtcgtc
       61 cagcaggtcc agcacttggt tggcgaacaa ctggtggccg tagggcgtgg ggtgaaaacc
      121 gtcggcaaac aggtagcgct gccaccagcc gctgccgctg ggcgcaccgg gcggcggcgt
      181 ggtggccgac agcgcgctgg ccgtgcattc ggcaaagctg cgctgcggca ccaccgtgac
      241 ccaagctgcg ccgcacaccg gcagcgtgac gttggtcagg ttgtacttgg cagggttcag
      301 cacctggtcg gtgaagcgcg tcgccaaatc caccaccttg acgcgcgact caccgctgaa
      361 attggccgcc agacgctggt tgaaggcgtt gacccagccc ttgaacagcg cttcggcctg
      421 cgcgcggccc gcggggccgg ccgccgaagc gccgatctgg tccagcacgg cctggaagcg
      481 cggcgtgtag gtgatggtgg gaatgttggc caccaccacc tgcttggcgc ccttgtccag
      541 cgcctgcgcc ttgatggtgc cggcaaagtt gtcggccagc gcctgcatgt acaggccacc
      601 ggcgttttcg gggccgttgg cgccggccag cacggtttgc agcgtggtgg ccggaatcag
      661 cgtgagcagc agcgcctggt aggcggcgcc ctgatcggcc gctgcgccca ggtaggcacc
      721 caccacatcc gctgcatcgt tgccgccgcc atcgaccagc accagatcct tggcgcccca
      781 gcccttggcg gcggcatctg tcagctgctt gccgatcgac agcggcgccg ccgcgccacc
      841 gctggccgcc gggttgttga tgcgcgcgcc gccgatggcg aagttggtgc aaccggcctg
      901 cgtgttggcc gcaaacgtgg tgccgttgaa cttgtagacc gggcacagcg gcgccagctc
      961 gtacgccttg gccacgcgct cgacccacaa cacgttcggc tcgtcggacg agccctgcac
     1021 gctggcaatg cgcgcgccgc ccggaatgcc ggagaaggtg ccgccgtcgc tgagtgaatc
     1081 accaatcacc ttgacggcgt tgatgccgag cgggttcttg tcgtcgtcgc caccgccgca
     1141 ggcggacagc gccacggcca ccgccagggc ggacagcttc cagttgagag agggcttgat
     1201 gtgcacgtga atgactcctc gggtgtgaat cggccatcgt aggcagtgcg gcgcgcgggc
     1261 gggttaggga ttacccaagc ggcgctatgc ttgccggcat gaacacagcg ccccgcaccc
     1321 accgcattgc cgtcatcccg ggcgacggca ttggccagga agtcatgcct gaggcgctgc
     1381 gcgcgctgcg agcggctgaa ggcaaattcg acttcaagct ggatctgacc cccatcacct
     1441 gggccagttg cgaccactac gccaagacgg gacaaatgat gcccgacgac tggaaggccc
     1501 agctcgagac gcaggatgcc atcctgttcg gcgccgttgg ctggccggcc acggtgcccg
     1561 atcacatctc gttgtggggc agtctgctga agtttcggcg cgagttcgac cagtacatca
     1621 acctgcgccc ggtgcgcctg ttcgagggcg tgccgtgtcc gctggcgggg cgcaaaccgg
     1681 gcgagatcga cttcctcgtc gtgcgcgaga acaccgaagg cgaatacacc aacctcggcg
     1741 gcgtgatgta cgccggcacc gagcgcgaaa tcgttgtgca agagaccgtg atgagccgct
     1801 ttggcgccga ccgcgtgctg aagttcgcct tcgagcaggc cgccgcgcgc ccgcgccaaa
     1861 agctcgacgt ggcgaccaag agcaacggca tcgccatcag catgccgtgg tgggacggcc
     1921 gcgccgacgc ggtgcatcaa agctacccgc acatcgcggt cgagaagcac cacattgaca
     1981 tcctcaccgc gcgcttcgtg ctgcagccgc agcgcttcga cgtcgtggtg gcgagcaacc
     2041 tgttcggcga catcctgagc gacctggggc cggcgtgcac cggcaccatc ggcctggcgc
     2101 cctcggccaa cctgaacccc gagcgcaagt tcccgagcct gttcgagccg gtgcacggca
     2161 gcgcgcccga catcttcggc caaggcattg ccaacccggt ggccatgatc tggtcggccg
     2221 cgatgatgct gcagtttctg ggcgagcagg ccgcgcacga cgccatgctg aaagccatcg
     2281 agcaggtgct ggcgaaaggg ccgcgcacgc cggatctggg gggcaaggcg tccaccacgg
     2341 agatgggtga agcgttggca gcattgatct aggactataa aaacaagagc tgctcgcgct
     2401 tgttaagcaa gggcaagagc ctgatttgat caaaaattca gatttagagt gaccgatgac
     2461 aaatgactgc gcctgcggcg catgacaaat gactgagcgc accattcggc gcggcacccc
     2521 tgtcatttgt catggagcgc agcgacgtca aggttttctc acggcgcgtc tcgcaccgcc
     2581 cattccacca gccgatccag cgccggccgc gccgcgccgg cgttggggtg gttgatgatg
     2641 ccgaccaccg cgtagttgcg gcccgacagg ccgtaggcgt agccggcaat cgccgtcacg
     2701 tcgcgcaagg tgccggtttt cagccgcgcg ttgccgatgg cggccgacga ggggctgcgc
     2761 gcggccatgc ggcgcgtggt gccgtccacg ccggcgatcg acagcgaatg ctcgaacacc
     2821 gcgccattcg ggtggccggc cgcttgttga agcagcgccg ccagtgacgc cgccgtgatg
     2881 cgctcgttgc gcgacaggcc cgagccgttt tcgacgatgg gcgtggtgcg caggccgaag
     2941 cggtcgcgcc accagcggcg cagcgtgttt tgcgattcgg ccaggctggc gcggccgtcg
     3001 cgcgccgaca gggtcagaaa cagctgctgc gccatcacgt tgttcgagaa cttgttgatg
     3061 tccgcaatga tgtcggccag cggcagcgaa aaacccgtca ccagcggctg gccggtggcc
     3121 gggcggtcgg accacttgac ctggccgttc aacgcgccgc cggcgccgcg ccacatcgcc
     3181 tcgatcacgc gcggcgcgta gctggcgggc tcgacgtagg caatgctcca gttctgctcg
     3241 ccgcaactcg tcgggtagcg gcccgagaag ctgacgcggt tggggtcggt gaaatcggcc
     3301 gccagccgcc cacgccagtt gccgcaggcg ccgcccaccg cgggcacttc ggtgggaatg
     3361 gccacgccgg ccattggcgg ctcgctttgc accagcacgc ggtgattggc cgggtcgggg
     3421 atgaacttga agaccagcgc cttgaagttg agcagcagcc cgtccggccc gacgttgtag
     3481 ggccgcagcg ggtcgtcgtc aaaggcggcg gcgtcgtgcg cgggcaggcg gaagatggtg
     3541 ttgtcgagca ggatgtcgcc gttgatctgg cgcacgccct tgtcctggat ggcgcgcatc
     3601 agctcctgca gccgttcaac caccagcttg gggtcgccgc tgccctggat cagcaggttg
     3661 ccattcagca ccccatcctt gatgtagccc tgcgtgtaga cccgcgtgcg ccagaaatgg
     3721 ctcggcccga gctggtcgac accggcgtag gtggtcacca gcttcatgac cgatgcgggg
     3781 ttcatcggca cgtcgggctg ccacgacagg cgcggcggcg gcagcgccat gctggcggcc
     3841 gcaggcgcgg cgttgcgctc gccactcggg ttgaccggct cgggcacgcg cgtgacggtg
     3901 ccgggtggcg gcggcagcgg cgccaccacc atcgccacgg cgctggtcgg cacgccggcc
     3961 ttggcgagcg cctgggcgat ctcgggcggc agcgtgggct gcgaccaggc gctggacgct
     4021 gtcaaggcac cgaacaaggc aggaatcagt tggcggaggg caaaacggca gcacatcacg
     4081 tccgcgagtc tagcggcgtg cgtaggggcg cggcggccgc gggccggccg gccgtggcgc
     4141 gtgcccggac tttgtcacgg ctcggcgcgg ccacgcgcgc ggatgccggg acgaggggca
     4201 gccgataatc ccctcatgcg tctttctccc cgtgcgaccg gcatcgcggc ggccgtgatc
     4261 acggtggcca tctggaccgg gttcatcgtc atcggccgcg cgtcggccgg gcgcacgctg
     4321 ctgccgttcg acatcggctt gctgcgcgtg ctgggcgcca gcgcggtgct gctgccctgg
     4381 ggctggtggc ttacgcgcgg ccggcgcgat acggcgtcgt cttcgctgct gggcctgtcg
     4441 ccgctgccgc tgcgccagac ggtgctggcg ggctttttcg gcggcttcat ctacgcggtg
     4501 ctgtgctacg cgggtttttt ctacgcgccg gcggcgcacg cctcggtgct gatgccgggc
     4561 agcctgccgc tgtggacggc gctgctggcc gcgctggtgc tgcgcgatcg catcacgccg
     4621 gcgcgcgcgc tagggctggc ctgcatcgtg gtgggcgatc tggtggtggg cggcgcaagc
     4681 ctgctgcagg ccttcaacgg cggcagcgtg tggaagggcg atgtgatctt catgagcgcg
     4741 gcgttttgct ggtcggtcta cagcgtgctg gcgcgcaagt tcgggctgga ggcggtgcgc
     4801 gccaccatcg ccatcacggc gtttgccttc ttcacctacg tgccgctgtt cacgctgctg
     4861 acggccagcg gcgtgctgcc cacgcatctg gccagcgcac catggggcga gatcgcgttt
     4921 caggcgctgt tccagggcgt gggctcggtg gtgatttcgg gcatcacctt cgtgcagatg
     4981 gtgcgcgcct tcgggccggt caaatcgacc atgatcacgg cgctggtgcc cggcctgtcg
     5041 gcgctgggcg cggtgctgct gctcggcgag ccgctgggct ggaacctgct ggcgggtctg
     5101 gccctcgtca ccgccggcat tctgtttggc gtgcgcgcgg cgcggcagac aactactgat
     5161 ttgatagctg ctcgcgcttg tccagcaagc gctggcgccc caaagcaatc atgacgcacg
     5221 tcgacctgaa cctgcttgcg ctcgacacca gcaccgatca gctgtcggtg gcggtgcagc
     5281 gcgccgatgg ccagcgcttt gcccacaccg gcgccggtgg cgcgcaggcg tcggccagct
     5341 tgatccccgc cgtgcaggcc ttgctggcgc aggccggtct gtcgctgagc gatttgagcg
     5401 ccatcgcctt cgggcgcggg ccgggctcgt tcaccggcct gcgcaccgcc tgctcggtgg
     5461 cgcaggggct ggcctggggc gcgggcgtgc cggtgctgcc catcgacacc ctgctggccg
     5521 tggccgaagc cgcgcgcagc gcgcatggcg ccacgcacgt gctggcgctg ctcgacgcgc
     5581 gcatgaacga ggtctacgcc gctgaatacg cgtggcgcga cgcggccggc tggcggcctt
     5641 tgagcaacat cgccgtgctg gcgcccgaag ccgtgcccac gcccggtgcg ggcgccgtgc
     5701 tggccggcaa cgcgtttgcc gcctacgccg atcgcctgcc cgccgcgctg caggcgctgc
     5761 cgcgcatcgc cgcgctgccc agcgccgacg ccctgctcaa cctggcgccc gcgctgctcg
     5821 ccgccggcgc cgccgtgccg gccgagcagg cgctgccgct ctacatccgc gataaagtgg
     5881 cgcagaccac cgccgaacgc agcgccgccg gcctggcccg ctgaccccat ccaccccatg
     5941 agcgccgtcc tgaagcccgc cccctccagc gccgtcgagg cacatctgga gccaatgacg
     6001 cccgagcggc tgccggccgt gctcgccgtc gagcaaagcg tgtacagcca cccgtggacg
     6061 cgtggcaact tcaccgatac gctggccgcc ggccaccacg cgctgtgcct gctgggcggc
     6121 gacgagctga tcggctactt cgtcgccatg cgcggctttg aggaagtgca tttgctcaac
     6181 atcaccgtcg cgccggcgca ccagcgccag ggctgggcgc tggtgctgct cgacgcgctg
     6241 gtgctgtggt cgcgcgcgca gggcgcgcag tggctgtggc tggaagtgcg cgccagcaac
     6301 acgcgcgcgc agcagatcta cctcaagcgc ggctttgccc gcgtcggctt gcgcaaggcc
     6361 tattaccccg ccggccacgg cctgcgcgaa gacgccgtgg tcatgaacct gcggctggcg
     6421 gacggcgcat gagcctggca ttggacgagc gccagcgcgc catgctggct gagatgggca
     6481 tcaagctgtg ggcgcccagc gcgccgcgcg tggcggaagt tgctcctgaa aaagaagctg
     6541 ctcgcgcagg tgagacaagc gctggcgccg aatttgattc aaagccgacg ccgacagcaa
     6601 gcctgcagac cgccgccgaa gcgcccatcg tgcggccacc cgtgcgcgcc attgccgcgc
     6661 cgctgccgac cgcgccggca gcccccgtgc ccaccgcgct cggcccgcgc cccgaaggcg
     6721 tggccgacat ggactggccg cagctcgaag ccaccgtcgc cgcctgccgc gcctgcggcc
     6781 tgtgcgccgg ccgcacgcaa accgtgttcg gcgttggcag ccgcaccgcc gactggatgg
     6841 tgatcggcga agcgccaggc gaaaacgagg accgccaagg cgagcccttc gtcggccagg
     6901 ccggcaagtt gctcgacaac atgctggccg ccatcggcct ctcgcggcag gtgggtgaca
     6961 ctatgaattc agtagctgct ggtggtgatt cagtaagggg cgacggccca aaagactcaa
     7021 aacaaggcgt ctacatcgcc aacgtgctca agtgccgccc gcccggcaac cgcaacccgc
     7081 aacccgacga aatcacccag tgcgagccct acctgcgccg tcaggtcgcg ctggtgcaac
     7141 cgaagatcat cctcgccatg ggccgctttg ccgtgcagtc gctgctgcaa accaccgaac
     7201 ccatcggcaa actgcgcggc cgcgtgcacc a
//
LOCUS       DAOMVO010000107         7218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2743_length_7218_cov_13.337987, whole genome shotgun sequence.
ACCESSION   DAOMVO010000107 DAOMVO010000000
VERSION     DAOMVO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2743_length_7218_cov_13.337987"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="PKV30_06760"
     CDS             complement(<1..1487)
                     /locus_tag="PKV30_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRL29953.1"
                     /translation="MQGAGVISPAAVAVDARPPAPADAGRGDPAWTLLDEQDEWLEFS
                     RPADAAEPGVWESTVVFEGMHCAACAGTIEQALRATPGVRAADVSAASHRGRVVWDES
                     ATQPSRWMNAVLRTGYRPLPAHDALATERRLAETRKMTWRLGVAGLCMMQVMMYATPT
                     YFTAPGEIEPDLVNLLRWAQWVLSVPVVLFSCQPFFRNAWLDLKLRRVSMDLPVALGM
                     LITFVVSSLGTFEPAGVFGREVYFDSLTMFVFFLLTGRWLELRLRDRTAGALEALMHR
                     LPDSVQRRDAAGAWERVSVRRIRAGDVLQVLPGEAFPADGVLINGQTLADEALLTGES
                     TPLARAAGERVIAGSHNLSAPVEMRVEQAGGDTRYAQIVALMAQAATSKPQIALLADR
                     MAKPFLIFVLLAAGLACAWWWPSDPGHALMIAVAILVVTCPCALSLATPAAMLASAGA
                     LARNGVLVRRLQALEALSHVDTVVFDKTGTLTRDAFVLEDVRLREG"
     gene            1645..2088
                     /locus_tag="PKV30_06765"
     CDS             1645..2088
                     /locus_tag="PKV30_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRL29954.1"
                     /translation="MYDRILVATDGSKLSKKAVDSAISLAALCGAELIALKVVPRYPQ
                     SYFEGSIPLSADDVARVEKQWAEDGQGVVDAVKKAGEAKGVKVKPILAKSDVVSDALI
                     AAAKRHKADIIVMASHGRKGVKRLLLGSETQQVLTHSEIPVLVLR"
     gene            complement(2208..2825)
                     /locus_tag="PKV30_06770"
     CDS             complement(2208..2825)
                     /locus_tag="PKV30_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRL29955.1"
                     /translation="MSLRSLLLLLVGAAIVAFIGVNWSAMTVPTDLNLIFTEIRAPLG
                     LVLLGLMALLSAVFVGLIAYTQGTVLMETRRHAKELSAQRELADKAEASRFTDLRAHL
                     DREVTRLSESIANNTRETLSRVDRAEMGLRDRPVDLEIGRLVQAVENHNRDLHSRVDR
                     LEMGLREGLVGYVPAPTSVRPAITGTPVPPSASVDDDPIGTTVPR"
     gene            complement(2936..4498)
                     /locus_tag="PKV30_06775"
     CDS             complement(2936..4498)
                     /locus_tag="PKV30_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HRL29956.1"
                     /translation="MSNQNLFVALRNAFPADLDSTAVETDDGLVYSWRDLDRATAMVA
                     NLLQSLDIPKSKDGVPPRVAVQVEKSVEAMVLYLATLRAGFVFLPLNTAYQSGEIEYF
                     IGNAAPAVVVCSGKNFPWVSPIAFKAGTRHVFTLNDDRSGSLLERAAHHSDVQTPAVR
                     RADDLAAILYTSGTTGRSKGAMLSHGNLLSNAVMLKDYWGWEKGDVLIHALPIFHVHG
                     LFVAIHGALLNGSKMLWHGKFDPKKVIADMPRATVFMGVPTLYVRMLAEPSLTKERAA
                     HMRLFISGSAPLLIETFNDWRQRTGHTILERYGMSETIMLTSNPYRADPRYGDQSERR
                     GGTVGFPLPGVSVRVRGDDGADLPAGDIGAIQVAGPNVFGGYWQMPEKTKEEFTGDGF
                     FKTGDVGKQDAHGYVTIVGRSKDLIISGGYNVYPAEIESVLNDMPGVQESAIVGVPHP
                     DFGEVGVAVVTPKPGAQLDGDALVAALKSQLANFKIPKRCFIEAELPRNTMGKVQKNL
                     LRERYQGLFSAA"
     gene            complement(4523..5323)
                     /locus_tag="PKV30_06780"
     CDS             complement(4523..5323)
                     /locus_tag="PKV30_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL29957.1"
                     /translation="MSGHVHLRRSDVDARVAVVTLSYRAKFNAMSRAMWRELAEVFGR
                     VQASDAWRCVLLQGEGGHFCAGGDIAEYPDFRFDAAQLRAFHEGDVWGGLSAMLDCDV
                     PIVAAIAGNCMGAGMEIASCCDIRVAADDAKFGAPIAKLGFPMAPREAQLVARVAGEL
                     TAREMLLEAGVLEAAEMKSRGFLHRVLAADALHAHALERAARIASLAPGAARLNKQTF
                     RALKTAPALEGQAQPAIENIVSTAYDYADSAEHREGIDAFLAKRPARF"
     gene            complement(5334..6317)
                     /locus_tag="PKV30_06785"
     CDS             complement(5334..6317)
                     /locus_tag="PKV30_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL29958.1"
                     /translation="MSLSISRRHALIAASAACALPLAARAQAAWPAKPVTLIVPFPAG
                     GGTDAFARPLATQFSKLTGKALVIDNRGGAGGTLGASVAAKAPADGYTLFMGGAHHVV
                     APSMYPKLDYDIEKDFVPLIQLASVPQVLVVNSKRANTGSYKEFIELIRKNPGKLDYA
                     SAGAGSVHHLAGELFKQQTKTSITHIPYRGAGPALQDLISGTVDMAFDGLGSSAGHIK
                     GGRIKALMVAGNKRNPAIPDVPSAAEVGLPAYDVHTWYGLWAPKGTPPEVQAAAIEAF
                     RKAAQDPEAKTVWAGQGADFPNVTGAQFGKLIHDEIRKWADVVKASGAKLE"
     gene            complement(6358..>7218)
                     /locus_tag="PKV30_06790"
     CDS             complement(6358..>7218)
                     /locus_tag="PKV30_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HRL29959.1"
                     /translation="DVGFLELQRISWHSPAALVEKLIRYEAVHDIKSWADAKNRLDED
                     RRCYAFFHPRLPGEPLIFVEVALLQGMADSITPLLDEEGAADDLDKATTAIFYSISNT
                     QTGLKGVSFGDSLIKRVVEELKGEFPQLKVFATLSPIPGLRAWLGKNAEAVLQAMPPR
                     ARQALARELGQPELEASALLKALDGVPELGEKSALRRWLLGAAARYLGRDLDARGRPL
                     DAVARFHLGNGARVERLNWLGDPSPKGLKQSYGLMVNYLYDLKRLDKHRALLAQGKVP
                     VSGAMESLQD"
BASE COUNT         1204 a         2453 c         2376 g         1185 t
ORIGIN      
        1 ccctcgcgca aacgcacgtc ttccagcacg aaggcatcgc gcgtcagcgt gcccgtcttg
       61 tcgaacacca cggtgtcgac gtgcgacagc gcttccagcg cctgcaggcg gcgcaccagc
      121 acgccgttgc gcgccagcgc accagcactg gccagcatgg ccgctggcgt ggccagcgac
      181 aaggcgcagg ggcaggtcac caccaatatc gccaccgcaa tcatcaatgc gtgccccggg
      241 tcgctgggcc accaccacgc acaggcgagg cccgcggcca gcaacacgaa gatcagaaac
      301 ggcttcgcca ttcgatcggc cagcagcgcg atctgcggct tgctggtggc ggcctgcgcc
      361 atcagcgcca cgatttgcgc atagcgcgtg tcgccgccgg cttgctccac acgcatctcg
      421 accggcgcgc tcaggttgtg gctgcccgca atcacccgct cgcccgcagc gcgcgcgagc
      481 ggcgtcgatt cgccggtgag cagtgcttcg tcggccagcg tctgcccgtt gatcagcacg
      541 ccgtcggccg gaaacgcctc gcccggcagc acctgcagca catcgcccgc gcgtatgcgg
      601 cgcaccgaca cgcgctccca ggcgcctgct gcatcgcggc gctgcacgct gtcgggcagg
      661 cggtgcatca gcgcctccag cgcgccggcg gtgcggtcgc gcaggcgcag ctccagccag
      721 cgcccggtca gcaggaaaaa cacgaacatg gtgagcgaat cgaaatacac ctcgcgcccg
      781 aacacgcccg cgggctcgaa ggtgcccagc gagctgacca cgaaggtgat caacatgccc
      841 agcgccaccg gcagatccat gctgacgcgc cgcagcttca agtccagcca cgcgttgcga
      901 aagaacggct ggcacgagaa cagcaccacc ggcaccgaca gcacccactg cgcccagcgc
      961 agcaggttca ccagatcggg ctcgatctcg cccggcgccg tgaagtaggt cggcgtggcg
     1021 tacatcatca cctgcatcat gcacaggccc gccacgccca ggcgccacgt catcttgcgg
     1081 gtttcggcca ggcggcgctc ggtggccagg gcatcgtgcg cgggcagcgg ccggtagccg
     1141 gtgcgcagca cggcgttcat ccagcgcgac ggttgcgtgg cgctctcgtc ccacaccacg
     1201 cggccacggt ggctggcggc gctgacgtcg gccgcgcgca cgcccggcgt ggcacgcaag
     1261 gcctgctcga tggtgccagc gcaggccgcg cagtgcatgc cttcgaacac gacggtcgat
     1321 tcccacaccc caggctcggc cgcatcggcc gggcggctga attcaagcca ctcgtcctgc
     1381 tcgtccagca gcgtccacgc ggggtcgcca cgacccgcat cggccggagc cggcgggcgc
     1441 gcatcgaccg ccacggccgc tggagaaatc acgcctgcgc cttgcatcac gcccggcact
     1501 gtacgccggt cgccggtggc gcagacttga catccgtcaa taaccaccgc ccttcatccg
     1561 ggtaagttcg gttatcgtag gtttggatac acaacgcggc cgccggacgc gaaccatcca
     1621 gcccgacccc gaaggagaac ccgcatgtac gaccgcatcc ttgtcgccac cgatggctcc
     1681 aaactgtcca agaaggccgt cgacagcgcc atcagcctgg ccgccctgtg cggagccgaa
     1741 ctgattgcgc tgaaagtggt gccgcgttat ccgcagagct atttcgaagg ctcgattccg
     1801 ctgtcggccg acgatgtggc gcgcgtcgag aagcaatggg ccgaggacgg ccagggcgtg
     1861 gtcgacgcgg tcaagaaggc gggtgaggcc aagggcgtga aggtcaagcc catcctggcc
     1921 aagtcggacg tcgtgtccga cgcgctgatc gccgccgcga agcggcacaa ggccgacatc
     1981 atcgtcatgg cctcgcacgg ccgcaaaggc gtcaagcgcc tgctgctggg cagcgaaacc
     2041 cagcaggtgc tgacgcactc cgagatcccg gtgctggtgc tgcgctgatc acggcgcttg
     2101 caactttttt tatagctgct cgcgctcagc tggcaaggga ttcaggccgt tttggcctaa
     2161 aacgcccatc tgacaagcgc gacgcgctat gaatgccaga gtgaggttca gcgcggcacc
     2221 gtggtgccaa tcggatcgtc gtcaacgctt gcagacggcg gcaccggcgt gccggtgatc
     2281 gcgggacgca cgctggtcgg cgcgggcaca tagcccacca agccttcacg cagacccatc
     2341 tccagccggt ccacccgcga atgcaggtcg cggttgtggt tttcaaccgc ttgcaccagg
     2401 cgcccgattt ccagatcaac cgggcgatca cgcaggccca tctcggcgcg gtcgacgcgc
     2461 gacagcgtct cgcgcgtgtt gttggcgatg ctttccgaca ggcgcgtgac ctcacggtcc
     2521 aggtgcgcgc gcaggtcggt aaagcgcgac gcctcggcct tgtcggccag ctcgcgctgg
     2581 gcagacaact ccttggcgtg gcggcgcgtt tccatcagca ccgtgccttg cgtgtaggcg
     2641 atcaggccga caaacaccgc cgaaagcagc gccatcaggc ccagcagcac caggccgagc
     2701 ggcgcgcgaa tctcggtgaa gatcaggttc agatcggtcg gcaccgtcat ggccgaccag
     2761 ttgacgccga tgaaggccac gatggccgcg ccgaccagca gcagcagcag ggatcgcagg
     2821 gacatttgga gttccttacc gtatggattt cctggtggcc gacgcggggc gacggccgtg
     2881 tgccgaagag tgtaacggcg ccaaaaagcg cgccatgtca gccaacgccg tcaaatcaag
     2941 cggcgctgaa aagaccctgg tagcgctcgc gcagcaggtt tttctgcacc ttgcccatgg
     3001 tgttacgggg cagttcggcc tcgatgaagc agcgtttggg gatcttgaag ttggccagtt
     3061 gcgacttgag cgcggccacc agcgcatcgc catccagctg cgcgcccggc ttgggcgtga
     3121 ccaccgccac gccgacttcg ccgaagtccg ggtgcggcac gccgacgatg gccgattcct
     3181 gcacgccggg catgtcgttc agcacgcttt cgatttccgc cgggtagacg ttgtagccgc
     3241 cactgatgat caagtccttg ctgcggccaa caatggtgac gtagccgtgc gcgtcctgct
     3301 tgcccacatc gccggtcttg aagaagccgt cgccggtgaa ctcttccttt gtcttctcgg
     3361 gcatctgcca gtagccaccg aacacgttcg gccccgccac ctgaatggcg ccgatatcgc
     3421 ctgcgggcag gtcggcgcca tcatcgccgc gcacccgcac gctgacgccc ggcagcggaa
     3481 agcccaccgt gccgccgcga cgctcgctct gatcgccgta gcgcgggtcg gcccggtacg
     3541 ggttgctggt cagcatgatg gtctcgctca tgccgtagcg ctccagaatc gtgtggccgg
     3601 tgcgctggcg ccagtcgttg aaggtttcga tgagcagcgg cgccgagccg ctgatgaaca
     3661 ggcgcatgtg cgcggcccgt tctttggtaa gcgacggctc ggccagcatg cgcacgtaga
     3721 gtgtcggcac gcccatgaac acggtggcac gcggcatgtc ggcgatcact tttttgggat
     3781 cgaatttgcc gtgccacagc atcttcgatc cgttcagcag cgcgccgtgg atggccacga
     3841 acaagccgtg cacgtggaag atgggcagcg cgtgaatcag cacatcgccc ttttcccaac
     3901 cccagtagtc cttcagcatc accgcgttcg acagcaggtt gccgtgcgac agcatcgcgc
     3961 ccttgctgcg gccggtggtg ccgctggtgt acaggatggc ggccaggtcg tcggcccgcc
     4021 gcaccgccgg cgtctgcacg tcgctgtggt gcgccgcgcg ctccagcagg ctgccgctgc
     4081 gatcgtcgtt cagcgtgaac acgtggcgcg tgcccgcctt gaaggcgatg gggctgaccc
     4141 acggaaagtt cttgccgctg cacaccacca ccgccggcgc ggcattgccg atgaagtact
     4201 cgatctcgcc gctctggtaa gcggtgttga gcggcaagaa cacaaagccc gcgcgcagtg
     4261 tggccaggta caacaccatc gcctcgaccg acttctcgac ctgcaccgcc acgcgcggcg
     4321 gcacgccgtc tttcgacttc gggatgtcga gcgactgcag cagattcgcg accatggccg
     4381 tggcgcggtc aaggtcgcgc catgaataga ccaggccgtc gtcggtctcg acggcggtgc
     4441 tgtccaggtc ggcggggaag gcgttgcgca gggcgacaaa gaggttctgg ttcgacatgg
     4501 tcaatcggat gaaagtcggg gtttaaaaac gcgccgggcg cttggcgaga aaggcgtcga
     4561 tgccctcccg atgttcggcg ctgtcggcgt agtcgtaggc cgtagatact atgttttcaa
     4621 tagctggttg cgcttgtccc tcaagcgctg gagccgtttt caatgctctg aacgtctgtt
     4681 tgttcaaacg cgcggcaccg ggggccagtg aggcgatgcg cgcggcgcgc tccagtgcat
     4741 gggcgtgcag cgcgtcggcc gccagcacgc ggtgcagaaa gccgcgcgac ttcatctcgg
     4801 cggcttccag cacgccggct tccagcagca tctcgcgtgc cgtcaactcg ccggccacgc
     4861 gcgccaccag ttgcgcctcg cgcggcgcca tgggaaagcc cagcttggca atcggcgcgc
     4921 cgaacttcgc gtcatcggcc gccacgcgga tgtcgcagca gctggcgatt tccatgccgg
     4981 cgcccatgca gttgccggcg atggcggcga cgatgggcac atcgcaatcc agcatcgccg
     5041 acaagccgcc ccacacgtcg ccttcgtgaa aggcgcgcag ttgcgccgcg tcgaagcgga
     5101 aatcggggta ttcggcaatg tcgccgccgg cgcagaagtg tccgccctcg ccttgcagca
     5161 gcacacagcg ccaggcgtcg ctggcctgga cacgcccaaa cacctccgcc aactcgcgcc
     5221 acatggcgcg cgacatggcg ttgaatttgg cgcgatagct cagcgtgacg acagccacgc
     5281 gggcgtcgac gtcgctgcgg cgcaaatgca catggcccga caaggcgcca gcctcactcc
     5341 agcttggcgc cggacgcctt caccacgtcg gcccacttgc ggatctcgtc gtggatcagc
     5401 ttgccgaact gcgcgccggt gacgttggga aagtccgcgc cctgcccggc ccacacggtc
     5461 ttggcttcgg ggtcttgcgc ggccttgcga aaggcctcga tggcggcggc ctgcacctcg
     5521 ggcggcgtgc ccttgggcgc ccacaggccg taccaggtgt gcacgtcgta cgcgggcagg
     5581 ccgacttcgg cggcgctcgg cacgtcgggg atggcggggt tgcgcttgtt gcccgccacc
     5641 atcagcgcct tgatgcgacc gcccttgatg tggccagcgg acgaacccag gccgtcaaac
     5701 gccatgtcca cggtgccgga gatcaaatcc tgcagcgcag ggccggcacc gcggtagggg
     5761 atgtgggtga tcgaggtttt ggtctgctgc ttgaagagct cgcccgccag atggtgcacc
     5821 gagccggcac cggccgaggc gtagtcgagc ttgccggggt tcttgcggat cagctcgatg
     5881 aattccttgt agctgccggt gttggcccgc ttgctgttca ccaccagcac ctgcggcacg
     5941 ctggccagct ggatcagcgg tacgaagtct ttctcgatgt cgtagtcgag cttggggtac
     6001 atgctggggg cgaccacgtg gtgcgcaccg cccatgaaca gcgtgtagcc gtcggccggc
     6061 gccttggccg ccaccgaggc gcccagcgtg ccgcccgcgc caccacggtt gtcaatgacc
     6121 agcgccttgc ccgtgagctt ggaaaactgc gtcgccagcg ggcgcgcaaa agcatcggtg
     6181 ccgccgccgg ccggaaacgg cacgatcagc gtgaccggct tggccggcca ggcggcttgc
     6241 gcgcgcgcgg ccaatggcag ggcgcaggct gcggatgcgg cgatgagcgc gtggcgccgg
     6301 ctgatggaaa ggctcatggg gtttgtctcc tgtcgtggtt ctcgaacatg ccagcggtca
     6361 atcttgcagg ctttccatgg cgccggatac ggggactttt ccctgtgcca gcagcgcgcg
     6421 gtgcttgtcc aggcgcttga ggtcatacag gtaattcacc atcaggccgt acgactgctt
     6481 gaggcccttg ggcgacgggt cgccgagcca gttcagtcgc tcgacgcgcg cgccattccc
     6541 taagtgaaag cgcgccacgg cgtccagcgg gcggccgcgg gcgtcgaggt cgcggcccag
     6601 atagcgcgcg gcggcgccca gcagccagcg gcgcagggcg gatttttcgc ccagctcagg
     6661 cacgccgtcc agcgccttca gcaacgcgct cgcctccagt tcgggctggc ccagctcgcg
     6721 cgccagcgcc tgccgcgcgc gcggcggcat ggcctgcagc accgcttcgg cgttcttgcc
     6781 cagccacgcg cgcaggccgg ggatgggcga gagcgtggca aacaccttga gctgcggaaa
     6841 ttcgcccttg agttcttcca ccacgcgctt gatgagcgaa tcgccaaagc tcacgccttt
     6901 caggccggtc tgcgtgttgc tgatggaata aaagatggcg gtggtggcct tgtccaggtc
     6961 gtcggcggcg ccttcttcgt ccagcagcgg cgtgatgctg tccgccatgc cttgcagcag
     7021 cgccacctcg acaaaaatca gcggctcgcc gggcaggcgc gggtgaaaga aggcgtagca
     7081 gcgccggtct tcgtcgagcc ggttcttggc gtcggcccag ctcttgatgt cgtgcaccgc
     7141 ctcgtacctg atcagctttt caaccagcgc ggcgggcgaa tgccagctga tgcgctgcag
     7201 ctccagaaaa ccgacgtc
//
LOCUS       DAOMVO010000108         7125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2795_length_7125_cov_15.493069, whole genome shotgun sequence.
ACCESSION   DAOMVO010000108 DAOMVO010000000
VERSION     DAOMVO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_7125_cov_15.493069"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..493
                     /locus_tag="PKV30_06795"
                     /pseudo
     CDS             170..493
                     /locus_tag="PKV30_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
     gene            complement(551..850)
                     /locus_tag="PKV30_06800"
                     /pseudo
     CDS             complement(551..850)
                     /locus_tag="PKV30_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930232.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            1040..1471
                     /locus_tag="PKV30_06805"
     CDS             1040..1471
                     /locus_tag="PKV30_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL29960.1"
                     /translation="MPNAAPPRALFHLAYNVTDLDTARRFYGGVLGCREGRSTDTWVD
                     FDFFGHQISLHLGEPFKTAPTGHVGDHLVPMPHLGLILQRADWQAMAKRLQAAGTDFV
                     IAPQLRFAGQPGEQWTMFFCDPFGNPIEIKGFEDLASVYAA"
     gene            complement(1511..2527)
                     /locus_tag="PKV30_06810"
     CDS             complement(1511..2527)
                     /locus_tag="PKV30_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRL29961.1"
                     /translation="MKLATYKDGSRDGQLLVVSRDLTTAHYATGIAQRLQQALDDWNF
                     ISPQLQDLYDALNAGRARHPFPFDAAQCMAPLPRAYQWADGSAYLNHVELVRAARGAE
                     VPESYFTDPLMYQGGSDDFLGARDDIRVADEAFGIDFEAELAVVTGDVPMAATPEQGL
                     EAIRLLMLVNDVSLRNLIPDELAKGFGFFQGKPSTAFGPVAVTPDELGDAWQEGRVHL
                     TLQISWNGRKVGLVDAGPDMRFHFGQLIAHAAKTRRLRAGSIIGSGTVSNPGTEKDGR
                     RDWPKGYACIAEKRAMETIQDGAPQTGYMKFGDSVRIEVKGKDGESVFGTIDQKVVES
                     GTGA"
     gene            2939..3754
                     /locus_tag="PKV30_06815"
     CDS             2939..3754
                     /locus_tag="PKV30_06815"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRL29962.1"
                     /translation="MPQRTVVAPRPAPPRAIRAEPLRVAVSAAPPTPEPEAESSPPAS
                     PAPSAPATPVAPAPTQLPGPAMLDYRVSGSARGIPFEADAQLIWRPGAGRYTAEWTVR
                     LPLVGARTQRSEGALTPAGLAPERYAEQARGERAAHFDAAGGRIRFSANTPDATLEPG
                     AQDRLSVSLQLAGLLAAAPARYPPGSVITLQTTGVREAGPWRWEVHEDETLQIDGQAV
                     PCAKLVRQPRGEYDNRIELWLARTLGHLPARLRVIQAGGDVADQVLRTLPAER"
     gene            3883..4164
                     /locus_tag="PKV30_06820"
     CDS             3883..4164
                     /locus_tag="PKV30_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HRL29963.1"
                     /translation="MNTLYDSDSYAVVHLLPDAPAGVPAQEGKPVLARHGFEIVDKRA
                     GKEVYLDGLWAELFQQRIAAWQENTPTQEEVEDTLEGYAELAQNPVVVH"
     gene            complement(4463..5533)
                     /locus_tag="PKV30_06825"
                     /pseudo
     CDS             complement(4463..5533)
                     /locus_tag="PKV30_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
     gene            5582..6427
                     /locus_tag="PKV30_06830"
     CDS             5582..6427
                     /locus_tag="PKV30_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRL29964.1"
                     /translation="MHQALRLILTLSAAWLLTACAAPARPLSAPEQARLAALLPADAL
                     LVGEQHDAPEHQQLQRQIVDWLAARGDLAALAMEMAERPHTTAGLPRDASESAVRAAL
                     AWDEAGWPWATYGPVVMAAVRAGVPVRGANLPRADMRRAMADTTLDARLPPAMLAEQE
                     SRIREGHCHALPESQIRPMTRIQIARDVAMAQTLTEAAAHGKAVLLVAGNGHVQRGLG
                     VPQHLPAGFESKVLSVQARQSQAAMNTEASDTPRRAGADLVWSTPTLPPRDHCAAFRP
                     AAAPR"
     gene            complement(6440..6907)
                     /locus_tag="PKV30_06835"
     CDS             complement(6440..6907)
                     /locus_tag="PKV30_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29965.1"
                     /translation="MTATSLKALMLAGATLLGAGATLALATAAQAQAYVNVQIGTPPP
                     PPRVEVMPAARPGYVWAPGHYQWAHNNYIWRRGHWVADRPGYTYVGPTWVSRGDHWVY
                     QPERWDMRPVRAQPPRQWDNRGRPPYGHPGWRDNDRDGVPNRYDRRPNDPYRR"
BASE COUNT         1116 a         2541 c         2407 g         1061 t
ORIGIN      
        1 ggcagcttgc cgttcttggc cttcatctgc aggtcgagca ccgcatcgtt cgcgccgcga
       61 cgggtgatgt tgaacgtgtt gtagcggctg tagtcgatgg ttttctggct cacgggcgtc
      121 tcctgatgat gatggcttga tcgtaagggc tgtgtcgata atcgaccgca tgagcgccgc
      181 gccaccgtcc gcctgcaccg tcggcctgca gaccgatggc cccgtcagca tcgtcacgct
      241 gcagcggccc gagcggctga acgccatcaa cgccgatttg ctgcgcgatt tgaacgcgca
      301 actgcagact cctcaggccg acgccgccac gcgcgccatc gtgctgaccg gcgccggccg
      361 cgctttttgc gctggcgatg atttgaaagc cttcgacgat caatccgcca cgccggccag
      421 catggcgcag atgtgcgaaa acgtgcagcg catcacgcgc acgttgatgg gcggccccaa
      481 gctgctggtg ggcgccgtgc acggctacgc cgtgggcgca ggcttcgagt ggctgctgaa
      541 ttgcgacctt aggccgcaca cccaggatgg tggccagcgc accgggcgtc aggccctgat
      601 cgagcgccag aaagtaggcg cactaatagc cgcccaccag cagcagcccg gtgccggcca
      661 cttgggcgcg cgtgcccggc tcaggcagcc agcgccgctc gcgccagccc agcagccaga
      721 gaaacaccag cgccacgcca aagcgcagcg ccagcaggac gaagggagag gcgtcgtcga
      781 gcccccagcg agaaaaaatg gcgccgctgc tccaaagcag cacgaacagc gccgtcggcc
      841 atgcattcat gaggattcac ctgtcgagga aacacacgct cgcgcccgcc gctatggtgc
      901 gggggccgcg agagcgaagt ggctgcgctt agccgcgcac gccgcgcaac ggcggggcag
      961 caggcggggg cgccgacagg tgatgcaagg tcatgcgcac atgatacggc ggcgccggcc
     1021 gtatcggtat gcttgcgcca tgcccaacgc cgccccgccc cgcgcccttt ttcatctggc
     1081 ctacaacgtc accgacctcg acaccgcgcg acgcttctac ggcggcgtgc tgggctgccg
     1141 cgaaggccgc agcaccgaca cctgggtcga tttcgacttc ttcggccacc agatttcgct
     1201 gcacctgggc gagcccttca agaccgcgcc cacgggccac gtgggcgatc acctggtgcc
     1261 gatgccgcac ctgggcctga tcctgcagcg tgccgactgg caggccatgg ccaagcggct
     1321 gcaggcggcc ggcaccgatt tcgtcattgc gccgcagctg cgctttgccg gccagccggg
     1381 cgaacagtgg acgatgtttt tctgtgaccc gttcggcaac ccgattgaga tcaagggttt
     1441 cgaggacctg gcgagcgtgt acgcggcgta gcgcgctatt cctgaattta tagctgctcg
     1501 cgcttatcca tcaagcgcca gtgcccgatt cgaccacttt ttgatcgatg gtgccaaaca
     1561 ccgattcgcc atccttgccc ttgacctcga tgcgcacgct gtcgccgaac ttcatgtagc
     1621 cggtctgcgg cgcgccgtcc tggatcgtct ccatggcgcg cttttcggcg atgcaggcgt
     1681 agcccttggg ccagtcgcgc cgcccgtctt tctcggtgcc ggggttgctg acggtgccgc
     1741 tgccaataat gctgccggcg cgcaggcggc gcgttttggc ggcgtgcgcg atcagctggc
     1801 cgaagtgaaa gcgcatgtcc gggccagcgt cgacgaggcc gaccttgcgg ccgttccagc
     1861 tgatctgcag cgtcaggtgc acgcggcctt cttgccacgc gtcacccagt tcgtcaggcg
     1921 tcacagccac agggccgaag gcggtggagg gcttgccttg aaaaaagccg aagcccttgg
     1981 ccagttcgtc cgggatcagg tttcgcaggc tgacgtcgtt gaccagcatc agcaggcgga
     2041 tcgcttccag cccctgctcc ggcgtggcgg ccatcggcac gtcgcccgtc accacggcca
     2101 actcggcctc gaagtcgatg ccaaaggctt catcggccac gcggatgtcg tcgcgcgcgc
     2161 ccagaaagtc gtcgctgccg ccttggtaca tcagcgggtc ggtgaagtaa ctctcgggca
     2221 cctcggcgcc gcgcgcggcg cgcaccagct cgacgtggtt caggtaggcc gagccgtcgg
     2281 cccactggta ggcgcgcggc agcggcgcca tgcactgcgc ggcgtcgaaa ggaaaagggt
     2341 ggcgcgcgcg gccggcgttc agcgcgtcgt acaggtcttg caactgcggc gagatgaagt
     2401 tccagtcgtc cagcgcctgc tgcaggcgct gcgcgatgcc ggtcgcataa tgggccgtcg
     2461 tcaggtcgcg cgagacgacc agcagttgtc cgtcgcgcga gccgtccttg taggttgcca
     2521 gtttcatggg tgataagtcg cgtgaatccc gcctgcgcgc ggtgccgctg gctggcctgc
     2581 gggaaacagg tgcggccacc gttggcaggt gtcgtcgccg cccgaagcaa tcgattctag
     2641 gcgagccgtt tcgtggcccg gcgcggtcat gagcgaccgg ccgggcgcgc gcccgtccct
     2701 cgcgctcgtg gcggcggtgg tgctggcgca tgggttgttg ttgcgaccag cgccgccgga
     2761 tcacccgggt cgtgtcgcgc caccggccgc gcccgcgttg gcgctgaggg tggttgatcg
     2821 gccgatcgca gcagctgaaa cgcgaaccgc cacgccgccg gcgcgccccg ttgcagcaac
     2881 cgtcgtcccg gtggctgcgc cgcctgcgcc tagaaagacc atcaccgccg ctcggccaat
     2941 gccgcagcgc actgtggtcg caccgcgccc ggcgcccccg cgcgcgatcc gcgccgagcc
     3001 cttgcgcgtg gcggtttcag ctgcgccgcc gacgcccgag ccggaggcgg aatcgtcgcc
     3061 ccccgcgtca ccagcgccgt ctgcgcccgc cacgccagtc gcccccgcgc cgacgcagct
     3121 gcccgggccg gccatgctcg actaccgcgt cagcggcagc gcgcgcggca tcccgttcga
     3181 ggccgacgcg caactcatct ggcgccccgg cgccggccgc tacaccgccg agtggacggt
     3241 gcgcctgccg ctggtcggcg cgcgcaccca gcgcagcgag ggcgcgttga cgcccgccgg
     3301 cctggcgccc gagcgctacg ccgagcaggc gcgcggcgag cgcgcggcgc atttcgatgc
     3361 cgctggtggg cgcatccgct tctcggccaa cacgcccgat gcgacgctgg agccgggtgc
     3421 gcaggatcgg ctcagcgtgt cgctgcaatt ggccgggctg ctggctgcag cgcccgcgcg
     3481 ctatccgccg ggcagcgtca tcaccctgca aaccaccggc gtgcgcgaag ccgggccgtg
     3541 gcgctgggaa gtgcatgaag acgaaacttt acaaatcgat gggcaggccg tgccgtgcgc
     3601 caaattggtg cgtcaaccgc gcggcgagta cgacaaccgc atcgaactgt ggctggcgcg
     3661 cacgctgggc cacctgccgg cgcgcctgcg cgtgattcag gcgggcggcg acgtggccga
     3721 tcaagtcttg cgcacgctgc cggctgagcg ttgaacccgc cgcgctcgcg cccttggctg
     3781 acatcgcgcc ggtacgctca cgtacacctt gaaagcccct gatgcgttgt catataggga
     3841 gcaagattgc tctgaacccc cccggaaagg ctcaaacccc tgatgaacac gctctacgac
     3901 tccgacagct acgcggtcgt tcacctgctg ccggacgcgc cggccggcgt gcccgcgcaa
     3961 gaaggcaaac ccgtcctggc gcgccacgga ttcgagatcg ttgacaaacg cgcgggcaaa
     4021 gaggtttatc tggacggcct gtgggccgag ttgttccagc agcgcattgc cgcctggcag
     4081 gaaaacacgc ccacgcagga agaggtggaa gacacgctgg aaggctatgc cgaactggcg
     4141 cagaacccgg tggtggtgca ctgatcgacc gcacccgcga agcggccccg tgcccgcgtc
     4201 gcgccccaag gcccgagcca cccgccgggc ctttttctat tgttttgata gctgatcgcg
     4261 cttgcccgac aagcgctggc ggctatattc atgacgaaat gacctcgctg cgcttcaatg
     4321 accgaatgac ttggggcttt tcggtcatgg cgcgcagcgc cgtcatcgaa ggccgccggc
     4381 cgaggtcatg cgccacaggc gtgtcatcaa aatccgtagc tgctggcgct ggagaggcaa
     4441 gcactgaagc cgcttttccc ttttattccc tgggcatcac ccagcgccga tagatcggct
     4501 cggccagcag cacagcgacc agtcggcaga cctcgaaagc cgtcaccacc gccacgccca
     4561 gttgcagcac cttggcggtg attgccatct cggcgatgcc accgggcgcg gtggccagca
     4621 tcagcgtcac cgggtgcagt ccgctcgccc acgccagcgc ggcgccaaac agcgcgctgg
     4681 cgccgatcat ggccagcgtg cccagtgcgg cggccgccag ccagcgcggc gcggcctgcg
     4741 gaaaccggcg cgtgaagcgc acgcgcaggc tgacgccgat gaccagttgc gccgcgttgg
     4801 tcagcgccgt cggcacgctg gacaggtgga catcggccat catcaaaccc atcgccgcca
     4861 gcagcgggcc gatgaaccac gggttggcgc gccccgtgag ccgcatcagc gccgcgccgg
     4921 cgcccgtggt cagcgccagc cacagcacgc ccggccattc ggccacgcgc gcggccggcg
     4981 gcgccacatc gagcgcctgc agttgccaat gctgcttggc ccattgcatg gccagcggca
     5041 cgctgagcac cgccatcgcc atgcgcacgc tgtgcgcagc ggccaccagg tcggtgcggg
     5101 cgccgcagcg ctcggccagc aacgtcatct acgacgcgcc gccgacggcg cccgaaaagt
     5161 acatcgtcgc cctggtttcc gcagcagacc agccctgaag atcggcgccc agcgcgcccc
     5221 gcaagaccaa gccgaagccc aggcccagca gcagcgccca gccaatgccg agcgcgacag
     5281 cccaccacag gctcagcacc agacgcgtca cctcgggcgt gaagtacagc cccagcgcgg
     5341 cgcctatggc ccactgcccg gcgttgcgca gcgccgcttc gctgtgcgtc ggcgcgcccg
     5401 ccagggtggc cagcgcggtc gccagcagcg ggccgatcat ccacggcacc ggcaggccca
     5461 gcgccaccgc cgcgcgcgcc gccagccagg ccagcagcag cgtgccaagc gtgcgcgggg
     5521 cgcggcgggc aatggcgaaa gcgactcggg catggcgcgt gtgggcgctt agtatggcgc
     5581 gatgcaccaa gcccttcgcc tcatcctgac gctcagcgcc gcatggctgc tgacggcctg
     5641 cgccgcgccg gcacggccgc tgtccgcgcc cgagcaggcg cgcctggccg cactgctgcc
     5701 ggccgatgcg ctgctggtcg gcgagcagca cgatgcgccg gagcatcagc aattgcagcg
     5761 ccagatcgtc gactggctgg ccgcgcgcgg cgatctggcc gcgctggcca tggaaatggc
     5821 cgaacgcccg cacaccaccg ccggcctgcc gcgtgatgcc agcgaaagcg cggtgcgcgc
     5881 cgcattggcg tgggacgagg ccggctggcc ctgggccact tacggcccgg tggtgatggc
     5941 cgccgtgcgt gcgggcgtgc cggtgcgggg cgccaatttg ccgcgtgccg acatgcgccg
     6001 cgccatggct gacacaacgc tggatgcgcg cctgccgccc gcgatgctgg ccgagcaaga
     6061 aagccgcatc cgcgaaggcc actgccacgc actgcccgag tcgcagattc gccccatgac
     6121 gcgcatccag atcgcgcgcg acgtcgccat ggcgcaaacg ctcaccgagg cggccgccca
     6181 cggcaaagcc gtgctgctgg tggccggcaa cgggcatgtg cagcgcggct tgggtgtgcc
     6241 gcagcacttg ccggcgggtt ttgaatccaa agtgctgtcg gtgcaggcga gacaatcgca
     6301 agcagctatg aataccgaag catccgacac gccacggcgc gccggcgccg acctggtctg
     6361 gtcgacaccg acgctgccgc cgcgcgatca ttgcgcggcg tttcgcccgg ctgcggcgcc
     6421 gcgctgaccg ccaacggtct cagcgccgat acgggtcgtt cggccggcgg tcgtaacggt
     6481 tgggcacgcc gtcgcggtcg ttgtcgcgcc aaccggggtg gccgtagggc ggccggccac
     6541 ggttgtccca ctgacgcggc ggctgcgcgc gcaccggccg catgtcccag cgctcgggct
     6601 gatagaccca gtgatcgccg cgcgacaccc aggtcggccc gacgtaggtg tagcccgggc
     6661 gatcggccac ccagtggcca cggcgccaga tgtagttgtt gtgcgcccat tgatagtggc
     6721 ccggcgccca cacgtagccg gggcgcgctg ccggcatcac ctcgacgcgc ggcggcggtg
     6781 ggggcgtgcc gatctgcacg ttgacgtagg cctgtgcctg cgccgccgtc gccagcgcca
     6841 gggtcgcacc ggcgcccagc agcgtggcac cggccagcat cagggccttg agggaggttg
     6901 cggtcatggt cttttccttc gaaaaactga acacgaccat tcaacgcgcc agcccggcca
     6961 tctgctgtaa accgcttgca aagctttgaa aagccgggtg tccgcccgct caccaggtgt
     7021 cgacgaagcg cccgccttcg acaggccgca cccgcgccga cgccagcccg cgcgccagcc
     7081 aggcgcgcgt gtcggccggg tcgatcacgg cgtcgatctc cagcg
//
LOCUS       DAOMVO010000109         7063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2835_length_7063_cov_14.525400, whole genome shotgun sequence.
ACCESSION   DAOMVO010000109 DAOMVO010000000
VERSION     DAOMVO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_7063_cov_14.525400"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..178)
                     /locus_tag="PKV30_06840"
     tRNA            complement(103..178)
                     /locus_tag="PKV30_06840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Glu,seq:ttc)
     gene            complement(206..281)
                     /locus_tag="PKV30_06845"
     tRNA            complement(206..281)
                     /locus_tag="PKV30_06845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:ggc)
     gene            complement(317..1723)
                     /gene="gltX"
                     /locus_tag="PKV30_06850"
     CDS             complement(317..1723)
                     /gene="gltX"
                     /locus_tag="PKV30_06850"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRL29966.1"
                     /translation="MTDKTDSTPRVRTRFAPSPTGFIHLGNIRSALYPWAFARAQGGD
                     FILRIEDTDLERSTQAAVDVIIEGMAWLGLDHDEGPFYQMQRMDRYKQVLGELQAAGH
                     VYPCYMSVAELDALRERQMAAKQKPRYDGTWRPEPGKTLPPVPEGVQPVLRFKNPQGG
                     VVAWDDKVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDIDMRITHVIRGDDHVNN
                     TPRQINIFRALGYEPPVYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGFLPDAMVNYL
                     ARLGWSHGDDEIFSRVQFLDWFNLDHLGRSAAQFDEAKLRWVNAQHLKAMDDEALARL
                     VAAQLEARGITPDERLPAICGLFKDRCDTTVVLTDWAQAYYADVSPSAADLGQHVSDA
                     IKPAIATLRDKLAGADWTLPGIGAAIKSTLTEHGLKMPQLAMPVRVLVMGARQTPSVD
                     AVLALHRREKVLARLQGA"
     gene            complement(1775..2989)
                     /locus_tag="PKV30_06855"
     CDS             complement(1775..2989)
                     /locus_tag="PKV30_06855"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRL29967.1"
                     /translation="MTVERTLPPDLHPDTLAVRVAIDRSQYGENSEALYLTTGFVQPN
                     SATSAERFQSGEGYTYARTSNPTVTAFERRLAALEGTQAAIGAASGMGAILMMIMGLL
                     KSGDHVVLSRSMFGSTLNLFAKEFGKFGVETTFVSQTDVAEWRAAVKPHTKLLFAETP
                     TNPLTEVCDIAALASIAHDAGALLAVDNAFATPVLQKPAALGADIVMHSGTKYLDGQG
                     RVMAGALCGSQALITEKFAPVVRTAGMVLSPFNAWVVLKGLETLGIRVKAQCEAALAM
                     ARWLEAHPAVARVYYPGLPSHPQHDLAMRQQNGLGGGVLAFVLAGDTPEQARARAFHV
                     IDSCRMLSIATNLGDTKTIISHPATTSHGRLTEEHRQAAGITQGVIRLAVGLEYVPDI
                     QADLSRGLDSVQ"
     gene            complement(3060..4565)
                     /gene="purF"
                     /locus_tag="PKV30_06860"
     CDS             complement(3060..4565)
                     /gene="purF"
                     /locus_tag="PKV30_06860"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRL29968.1"
                     /translation="MCGIVGVVSNAPVNQLIYDALLLLQHRGQDAAGIVTQQDRKFHM
                     HKAKGMVRDVFRTRNMRALPGHSGLGQVRYPTAGDAYNEEEAQPFYVNAPFGIVLVHN
                     GNLTNADALKAELFTTDHRHINTESDSEVLLNVLAHELGRATRGAALGPDEVFAAVSA
                     VHRRIRGSYAVIAQIAGRGMLAFRDPFGIRPLNQGRSADGGVMLASESVALEGTGFMP
                     ERDVAPGEAVFVTLDGTVHTRQCAPQAQLSPCIFEYVYLARPDSVLDGISVYQARMNL
                     GETLAKRVISTVPPDQIDVVIPIPESSRPSATELARLLGKPYREGFVKNRYVGRTFIM
                     PGQAVRKKSVRQKLNAIASEFAGRNVLLVDDSIVRGTTSKEIVQMAREAGARKVYLAS
                     AAPPVRYPNVYGIDMPTKDELVAHGRSVEEIRQIIGADALIYQDVDAMKRAIGQLNPS
                     LRGFDASCFDGVYVTGDITEGDIDRMNQQRVKTDEGEAEGSRLTLPNAFVA"
     gene            complement(4639..5190)
                     /locus_tag="PKV30_06865"
     CDS             complement(4639..5190)
                     /locus_tag="PKV30_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRL29969.1"
                     /translation="MTLSAIDWLALTVLIASLLLGAWRGLLYEVLSLAGWVVAFLAAR
                     WAAPVVGDWLPMGESSEPLRYAAGFALVFIATAFLCGMLAALARHAAKAIGVRPVDRL
                     FGAVFGVLRGVLVLLVLAALVSLTPLRDEAWWRQSQSAPWLEMALMHLHPWLPDALGR
                     QQPMTPPAGEPVASMAVPLTRHS"
     gene            complement(5242..6240)
                     /locus_tag="PKV30_06870"
     CDS             complement(5242..6240)
                     /locus_tag="PKV30_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL29970.1"
                     /translation="MAFFKFRQRGQPQPEPTRGRGRKNEPAAPGPQETIDTLRRRARH
                     RLIGAAVLVGLAIIGFPLLFDTQPRPVAVNAPITIPDKDKVAPLRTPDPVPAAASLGE
                     REEMVSGAVAPRGGERTASASAAAVAPARRASAEKPAAPTRDARPDAAAADARAKAEE
                     AARLKAEEAARAKAAQAKREEEAKAAQARRDDDRSQAQTDAQAKREEAARAKREQEAR
                     AKREAELRAKREEAARARALLEGRGTPSADKPAAADAKGGRFIVQVGAFADDSAVREA
                     RQKAERAGVKTYTQTVETSAGKRTRVRAGPFSSREDADKAAGALKKAGLGGSVLSL"
     gene            complement(6252..>7063)
                     /locus_tag="PKV30_06875"
     CDS             complement(6252..>7063)
                     /locus_tag="PKV30_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRL29971.1"
                     /translation="ELLGDTREKIGFEKAGILRTGRPAIVSDPVPPQSVIDRATEIGA
                     DLWLLGRDFNYSGDKQQWAWAGRGRRYAGLAYPALRGANQLINASGVLAALTAMRQQL
                     PVTAQAVRNGLALVDLPGRFQVVPGQPALVLDVAHNPHSVAALALNLDAMGFYPTTHA
                     VFGAMADKDLTPMLQRMDPLVDRWYFTDLPTPRAASGAALQAAWQAVTKRTDAVSAVF
                     STPLDALHAAAAKAQAADRIVVFGSFYTVGGVLEHGTPRLQAGAAAQGPRG"
BASE COUNT         1061 a         2348 c         2440 g         1214 t
ORIGIN      
        1 ccgcgacccc cggcgtgaca ggccggtatt ctaaccgact gaactaccgc tccgcgtcgg
       61 tgtgacgttt gctctcttgc gagagccaag tgccacaaaa cttggcgacc ctacggggat
      121 tcgaaccccg gtactcaccg tgaaagggtg atgtcctagg cctctagacg atagggtcaa
      181 aacctagaat cttcttcgac aaccttggtg gaggtaagcg ggatcgaacc gctgacctct
      241 tgcatgccat gcaagcgctc tcccagctga gctatacccc ctctcgggtg tcgagccttg
      301 cattatatgt cgaaaatcag gcgccttgca gtcttgccaa gactttttcg cggcgatgca
      361 acgccagcac ggcatcgacc gacggcgtct ggcgcgcgcc catgacgagc acgcgcaccg
      421 gcatggccag ttgcggcatt ttcaggccgt gctcggtcaa ggtgctcttg atggcggcgc
      481 caatgcccgg cagcgtccaa tccgcgcccg ccagcttatc gcgcagcgtg gcgatggccg
      541 gcttgatggc gtcgctgacg tgctggccga ggtcggccgc gctcggcgag acatcggcgt
      601 aataagcctg cgcccagtcg gttaacacca ccgtcgtgtc gcagcggtcc ttgaacaggc
      661 cgcagatggc gggcaggcgc tcgtcgggcg tgatgccgcg cgcctcaagc tgcgcggcca
      721 ccagccgcgc cagcgcctcg tcgtccatcg ccttcaagtg ctgggcgttg acccagcgca
      781 gcttggcttc gtcgaattgc gcggcgctgc gacccaagtg gtcgaggttg aaccagtcga
      841 gaaactgcac gcggctgaag atctcgtcgt cgccgtggct ccagcccaga cgcgccaggt
      901 agttgaccat ggcgtcgggc aaaaaaccct cgtcgcggta ttgggtgacg ggcttggcgc
      961 cgttgcgctt gctcatcttt tcgccctgct cgttcagcac ggtgggcagg tgcgcgtaca
     1021 cgggaggctc gtaacccagg gcgcggaaga tgttgatctg gcgcggcgtg ttgttgacgt
     1081 ggtcgtcacc gcggatcacg tgggtgatgc gcatgtcgat gtcatcgacg acgacgcaga
     1141 agttgtacgt cggcgtgcca tccggccgcg caatcaccag gtcgtccagc tcgtcgttgc
     1201 tgatctcgat gcggcctttg accttgtcgt cccacgccac cacgccgcct tgcgggttct
     1261 tgaagcggag cacgggctgc acgccttcgg gcacgggcgg cagcgtcttg ccgggttcgg
     1321 gccgccaggt gccgtcgtag cgcggttttt gcttggcggc catctggcgc tcgcgcaggg
     1381 cgtcgagttc ggccacgctc atgtagcaag ggtagacgtg gccggcggcc tgcaattcgc
     1441 caaggacttg cttgtagcga tccatgcgct gcatttgata gaacgggcct tcgtcgtggt
     1501 cgaggccgag ccaggccatg ccttcgatga tgacgtcgac cgccgcttgc gtggagcgct
     1561 ccaggtcggt gtcttcgatg cgcaggatga agtcgccgcc ctgcgcgcgc gcaaaggccc
     1621 acgggtacag cgccgagcgg atgttgccca ggtggatgaa gccggtgggc gatggcgcaa
     1681 agcgcgtgcg cacgcggggg gtggaatcgg ttttatcggt catatgggcc tctggcgctt
     1741 gtgtatccag cgcgagcagc tatcaattca gtagttattg aacactgtcc aggccacgcg
     1801 aaaggtcggc ctggatgtcg ggcacgtact ccagccccac cgccagccgg atcacgcctt
     1861 gcgtgatgcc ggcggcttgc cggtgttctt cggtcagccg gccgtgcgag gtggtcgcgg
     1921 ggtggctgat gatggtcttg gtgtcgccca ggttggtggc aatcgacagc atgcggcagc
     1981 tgtcgatgac gtgaaacgcc ctggcgcgcg cctgctcggg tgtgtcgccc gccagcacga
     2041 aggccagcac gccaccgccc agcccgttct gctggcgcat cgccagatcg tgctgcgggt
     2101 gcgagggcaa gccggggtag tagacgcgcg ccacggccgg gtgcgcctcc agccagcgcg
     2161 ccatggcaag cgccgcctca cactgcgcct tgacgcgaat gcccagcgtc tccagccctt
     2221 tcaacaccac ccaggcgttg aaaggcgaca acaccatgcc ggccgtgcgc accacgggcg
     2281 cgaacttttc cgtgatcagc gcctggctgc cgcacagtgc gccggccatc acgcggcctt
     2341 gcccgtcgag gtacttggtg ccggaatgca tgacgatgtc ggcgcccagc gcggcaggct
     2401 tctgcagcac cggcgtggca aaggcgttgt ccaccgccag caatgcgcct gcgtcgtgcg
     2461 ctatggaagc cagagcagcg atgtcgcaca cctcggtcag cgggttggta ggcgtttcgg
     2521 caaacagcag cttggtgtgg ggcttgacgg cggcgcgcca ctcggccacg tcggtctgcg
     2581 agacgaaggt ggtctcgacg ccgaacttgc cgaattcctt ggcgaacagg ttcagcgtgg
     2641 agccgaacat cgagcgagac agcaccacgt ggtcgccgct tttcagcagc cccatgatca
     2701 tcatcaggat cgcgcccatg ccgctggccg cgccgatggc ggcttgtgtg ccctccagcg
     2761 cggccaagcg ccgctcgaaa gcggtcacgg tggggttgga agtgcgcgcg taggtgtagc
     2821 cctcgccgct ctggaaccgc tcggccgacg tcgccgaatt gggctgcacg aagccggtgg
     2881 tgaggtacag cgcctcggaa ttctcgccgt actggctgcg gtcgatcgcc acccgcacgg
     2941 ccagcgtgtc ggggtgcaga tcgggcggca aggtgcgttc cacggtcatc gaaatactct
     3001 gttttttata gctgcttgcg cttgatggac aagcgccgga ggccgaaatg ccttgaaaat
     3061 caggcgacga aggcgttggg cagcgtcagg cgcgagccct cggcttcgcc ctcgtccgtc
     3121 ttcacgcgct gctgattcat gcggtcgatg tcgccttcgg taatgtcgcc ggtcacgtac
     3181 acgccgtcga agcacgaggc atcgaagccg cgcagcgacg ggttcagctg gccgatggcg
     3241 cgcttcatgg cgtccacgtc ctgatagatc agcgcgtcgg cgccgatgat ctggcggatt
     3301 tcttccacgc tgcggccgtg cgcgaccagc tcgtccttgg tcggcatgtc gatgccgtag
     3361 acgttgggat agcgcaccgg cggcgcagcg ctggcgaggt acaccttgcg cgcaccggct
     3421 tcgcgcgcca tttgcacgat ctctttcgac gtcgtgccgc gcacgatgga atcatcgacc
     3481 agcagcacgt tgcggccggc aaattcactg gcgatggcgt tgagcttttg ccgcaccgat
     3541 ttcttgcgca ccgcctgccc cggcatgatg aaggtgcggc ccacgtagcg gttcttgacg
     3601 aagccttcgc ggtacggctt gccaagcagg cgcgccagtt cggtggcgct gggccggctg
     3661 gattcgggga tcgggatcac cacgtcgatc tggtctggcg gcacggtgga aatcacgcgc
     3721 ttggccagcg tctcgcccag gttcatgcgc gcctggtaga ccgagatgcc gtccagcacc
     3781 gaatcgggcc gcgccagata gacgtattca aaaatgcacg gcgacagctg cgcctgcggc
     3841 gcgcactggc gcgtgtgcac cgtgccatcg agcgtgacaa acaccgcctc gcccggcgcc
     3901 acgtcgcgct cgggcatgaa gccggtgcct tccagcgcca cgctttcgct ggccagcatc
     3961 acgccgccat cggcgctgcg cccctggttg agcgggcgga tgccgaacgg atcgcgaaac
     4021 gccagcatgc cgcgcccggc gatctgggcg atcacggcgt aagagccgcg aatgcgccgg
     4081 tgcacggcgc tgacggcggc aaacacctcg tccggcccca gcgcggcgcc gcgcgtggcg
     4141 cgccccagct cgtgcgccag cacgttgagc agcacttcgg aatcgctctc ggtgttgatg
     4201 tggcggtggt cggtggtgaa cagctcggcc ttcaacgcgt cggcgttggt caggttgccg
     4261 ttgtgcacca gcacgatgcc gaacggcgcg ttgacgtaga agggttgcgc ttcttcctcg
     4321 ttgtacgcgt cgccggccgt cgggtagcgc acctggccca ggccgctgtg gccgggcagc
     4381 gcgcgcatgt tgcgcgtgcg aaacacgtcg cgcaccatgc ccttggcctt gtgcatgtgg
     4441 aacttgcggt cctgctgcgt gacgatgccg gccgcgtcct ggccgcggtg ctgcagcagc
     4501 aacagcgcgt cgtagatcag ctggttgacg ggtgcgttgc tgacaacgcc gacgatgccg
     4561 cacatggtgg gggttccggg ctcaagatgg gtgaatggtc gattgtcggg ccgaaagcga
     4621 ttcaccggct cgccccgatc acgaatgacg agtcaatggc acggccattg acgcgaccgg
     4681 ttcgccggct ggcggtgtca tcggctgctg cctgcccaac gcatctggca accaagggtg
     4741 gaggtgcatc agcgccattt caagccaggg cgcgctttgc gactggcgcc accacgcctc
     4801 gtcgcgcagc ggcgtcagcg acaccagcgc ggccagcacc agcagcacca aaacgccgcg
     4861 cagcacgccg aacacggcgc caaacaggcg atccaccggc cgcacgccga tcgccttggc
     4921 cgcgtggcgc gccagcgcgg ccagcatgcc gcacaagaaa gcggtggcaa tgaacaccag
     4981 cgcaaagccg gccgcgtagc gcagcggctc ggacgattcg cccatcggca accaatcgcc
     5041 caccaccggc gccgcccagc gcgcggccag aaaagcgacc acccagccgg ccagcgacag
     5101 cacctcgtac agcaggccgc gccaggcgcc cagcagcagc gaggcgatca gcacggtcag
     5161 cgccagccag tcgatggcgg acagggtcat gcggcgcttt cgcggtgggg cggccgcagc
     5221 ggtggcaagg cgcgcttcac gtcacaacga cagcaccgag ccgcccaggc cggctttttt
     5281 cagcgcgccg gcggccttgt cggcgtcttc gcggctgctg aacgggccgg cgcgcacgcg
     5341 ggtgcgcttg ccggcggacg tttccacggt ctgggtgtag gttttgacgc cggcccgctc
     5401 ggccttctgg cgcgcctcgc gcacggcgct gtcgtcggca aacgcgccca cctgcacgat
     5461 gaagcggccg cccttggcat cagcggcggc tggcttgtcg gctgaaggcg tgccgcggcc
     5521 ttccagcagg gcgcgggcgc gcgcggcttc ctcgcgtttg gcgcgcaact cggcctcgcg
     5581 cttggcgcgg gcttcctgct cgcgcttggc gcgcgccgct tcttcacgct tggcctgggc
     5641 atcggtctgc gcctggctgc ggtcgtcgtc gcgccgggcc tgggcggcct tggcttcttc
     5701 ttcgcgcttg gcctgggcgg ctttggcgcg cgcggcttcc tcggccttca ggcgggcggc
     5761 ttcttcggcc ttggcgcgcg catcggcggc tgcggcgtct ggccgcgcat cgcgggtcgg
     5821 ggcggcgggt ttttcggcgg acgcgcggcg cgccggcgcg acggccgctg cgctggcgct
     5881 ggcggtgcgt tcgccgccgc gtggcgccac ggcgccgctc accatctcct cgcgctcgcc
     5941 caggctggcg gcggccggca ccgggtcggg cgtccggagg ggcgccacct tgtccttgtc
     6001 gggaatggtg atgggcgcat tcaccgccac cgggcgcggc tgggtatcga acagcaacgg
     6061 aaagccgatg atggccagcc ccaccagcac cgccgcgccg atcaggcggt ggcgcgcgcg
     6121 gcggcgcagc gtgtcgatgg tttcctgcgg gccgggcgcg gccggttcat tcttgcggcc
     6181 acggccgcgg gtcggctcgg gttggggctg gccgcgctgg cgaaacttga aaaatgccat
     6241 gaattcctgg gtcagccgcg ggggccctgc gcggcggcgc cggcttgcag gcgcggggtg
     6301 ccgtgctcca gcacgccgcc cacggtgtag aacgatccga agacgacgat tctatctgcc
     6361 gcctgcgcct tggccgccgc cgcgtgcaag gcatcaagag gggtcgaaaa cacggctgag
     6421 accgcgtccg tgcgcttcgt taccgcctgc caggcggcct gcagcgcggc gccgctggcc
     6481 gcgcgtgggg tgggcaaatc ggtgaagtac cagcggtcaa ccagcgggtc catgcgctgc
     6541 agcatcggcg tcaaatcctt gtcggccatg gcgccaaaaa cggcgtgcgt ggtggggtaa
     6601 aaacccatcg catcgaggtt gagcgccagc gccgccaccg aatgcgggtt gtgcgccacg
     6661 tccagcacca gcgccggctg gccgggcacg acctgaaagc gcccgggcag atcgaccagc
     6721 gccagcccgt tgcgcaccgc ttgcgccgtc accggcagct gctggcgcat cgccgtcaat
     6781 gccgccagca cgcccgatgc gttgatgagt tggttggcgc cgcgcagcgc gggatacgcc
     6841 agcccggcgt agcgccgacc gcgcccggcc caggcccatt gctgcttgtc gcctgaatag
     6901 ttgaagtcgc gccccagcag ccacaggtcg gcgccgatct cggtcgcgcg gtcgatcacg
     6961 ctttgcggcg gcaccgggtc gctgacgatg gcgggccggc cggtgcgcag gatgccggct
     7021 ttctcgaagc cgatcttctc gcgcgtatcg cccagcagtt cgg
//
LOCUS       DAOMVO010000110         7023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2862_length_7023_cov_12.522675, whole genome shotgun sequence.
ACCESSION   DAOMVO010000110 DAOMVO010000000
VERSION     DAOMVO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_7023_cov_12.522675"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..985
                     /locus_tag="PKV30_06880"
     CDS             173..985
                     /locus_tag="PKV30_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL29972.1"
                     /translation="MNAPEAPFALPGAGLRAAATQARAAAQVQPLIALDDVTVRYGAV
                     HALQGVVLRIAPGDSVALIGGNGSGKSTLLRVVHGLIAPSSGQCQAPPRAAQAMLFQR
                     PWMLRASARHNIAVGLWLRGAPWRAAQARALQALQRVQLADVADRGARGLSGGQQQRL
                     ALARAWAQQPQLLLLDEPTASLDPHAKHEVEALMQEFSSAPTADSEAASAPLTMLFAS
                     HNLGQVKRLARRVVYLEQGRVLADLPTEDFFNRALLRSVSPQADWFLKGELS"
     gene            982..1812
                     /locus_tag="PKV30_06885"
     CDS             982..1812
                     /locus_tag="PKV30_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL29973.1"
                     /translation="MSFITSFWPLAHARQALVAIGLVAFSVGASAQSIVVASTTSTEQ
                     SGLFGYLLPEFKKATGIEVKVVALGTGQALDTARRGDADAVFVHDVQAEQKFVAEGFG
                     VKRYPVMYNDFILVGPKNDPAGVRGKDIVEGLKKLAAANAAFISRGDKSGTHAAELRF
                     WDQAGLKDAKGSGYKECGCGMGPALNMAASTGAYVLADRGTWLNFKNRADLAILVEGD
                     KRLFNQYGVMVVNPAKHPQVKAAEAQKFVDWLVSPAGQSVIAGYKIGGEQLFFPNAEK
                     "
     gene            1967..2272
                     /locus_tag="PKV30_06890"
     CDS             1967..2272
                     /locus_tag="PKV30_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29974.1"
                     /translation="MPTPTSITQCLLGLGLAAALLPAAAQTTVLINPGDQIEGDRLAV
                     FNTWKNALDASLRKVGAANVQTRFSSDATADLSATRANTFDVIVAPAPTIGSAVRHG"
     gene            2282..3157
                     /locus_tag="PKV30_06895"
     CDS             2282..3157
                     /locus_tag="PKV30_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL29975.1"
                     /translation="MGLADTARTVLVAPADSPISDLAQSQGKRVGMPGQDSVVTYLLR
                     GEVQANNMTLRKKYAAVYQTRYQDALLQCLQLRRCDVVAVEHSVAKRWIAAGDKVKIV
                     WQSREVPGQSVAVRKGANLSVDALRSALMTELPKASAVSYTDARTLKDFDYVSSLGYF
                     TPRVLAGATLVEDPAMIETLMAGGAQYIDTRTQAEFDRGHVPGARLVPYVEKSAKDPD
                     YDAAHDQFKPQDLGADKSKALIFACNGAECWKSFKASHAAMKAGYSKVYWFRTGFPAW
                     RASGRKVDGDAAKVG"
     gene            complement(3166..4272)
                     /gene="modC"
                     /locus_tag="PKV30_06900"
     CDS             complement(3166..4272)
                     /gene="modC"
                     /locus_tag="PKV30_06900"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HRL29976.1"
                     /translation="MNAAALQVRARVAFGSGPDAFTLDVDLPLPGAGITALFGPSGCG
                     KTTLLRAVAGLVRPQPGRVVVGDQMWQDDAAGVWLPTHRRALGYVFQEASLFAHLLVQ
                     GNLDFGLKRVPAAERRIALDQAIELLGIGHLLARRPSQLSGGERQRVAIARALATSPR
                     LLLMDEPLAALDAARKAELLPYFERLQRELRIPLLYVSHSLDEVARLATHMVLLDAGR
                     VSANGPTGELLTRLDLPLAHGDHAGAVIDGALAAIDQRWNLLEVRFAGGVLHCVQAAG
                     SPPRQVGERLRLRVLARDVSLALAPASDTSILNVLPATVHSLTDDGPAQTLVALDLAG
                     STLLARVTRKSAAALRLSAGQRVFAQVKGVALLD"
     gene            complement(4269..4946)
                     /gene="modB"
                     /locus_tag="PKV30_06905"
     CDS             complement(4269..4946)
                     /gene="modB"
                     /locus_tag="PKV30_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRL29977.1"
                     /translation="MLDATSLAAIRLSIELSVVTTLSLLVLGTPLAWWLARGRSRWRL
                     PIRSLVALPLVLPPSVLGFYLLVTLGANGPIGQITQSLGWGTLAFTFWGLLIGSLVYS
                     LPFVVQPLINAFEAVGERPLEAAATLRAGPLDAFWHVALPLARPGYLTGAVMGFAHTL
                     GEFGVVLMIGGNIPGQTRVISVQIYDHVEALEYTQAHWLAGGMLVFSFGVLLTLHWLQ
                     PPTRRRA"
     gene            complement(5060..5818)
                     /gene="modA"
                     /locus_tag="PKV30_06910"
     CDS             complement(5060..5818)
                     /gene="modA"
                     /locus_tag="PKV30_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL29978.1"
                     /translation="MRFNLPRLLTLTAALALSAAAQAEVVQIAVAANFTAPARALAEI
                     FARTTGHEAKLSFGATGAFYTQIKNGAPFDILLAADDERPIRLEKEGDTVPGSRFTYT
                     IGQLVLWSAKPGLVDGEGAVLKGGNFNKIAIANPKLAPYGAAAVETMDKLGLAAALTP
                     KLVTGESIGQTYNFIATGNAELGFVALAQVLDGGKMKSGSMWVVPAKYHAPIIQDAAI
                     LKRAAANPAAKAWMALMRSPNTQDFIRSYGYEVK"
     gene            5927..6682
                     /locus_tag="PKV30_06915"
     CDS             5927..6682
                     /locus_tag="PKV30_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRL29979.1"
                     /translation="MGKSLVSAAALGHGPADKRMEILHLVGRHGSISQAARSAGVSYK
                     AAWQAIHTLTNLAGEPLVDSSVGGAGGGGARLTPAGERLLAAAEQLDSARRRVLARVN
                     GAESAALAGPRTSMRNHLRAVVERLESDGARDPMVRVLLGLAGGGTLASIITRESAEL
                     LGLAAGLPVLALCKATAVRVHAAEAAAPMGGNRLPGRVLRVSRGVQRDEVVVTLAGDQ
                     QLVGFAARPNRLRAGSRTDAVLDDSAVVLALTS"
     gene            complement(6770..6895)
                     /locus_tag="PKV30_06920"
     CDS             complement(6770..6895)
                     /locus_tag="PKV30_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29980.1"
                     /translation="MNTPSSKPACAARVVMLELISALPAGAPMDDALFPLLWATG"
BASE COUNT         1174 a         2400 c         2446 g         1003 t
ORIGIN      
        1 ccaccgccat cgcgctggaa accagcaagg gcgatctgcc gctggcgctg ggcctggggc
       61 tggtgctgct caccgtggtg ctgctgctga acacgctgat cgcgctggtg cgccactggc
      121 gcgagcgggt cgacggcctg gccgccccgc tggtgcgcaa ctggttgccc gcatgaacgc
      181 gccagaggcc cccttcgcgc tgccgggcgc cggcttgcgg gccgccgcga cgcaggcgcg
      241 cgcggcggcg caggtacaac cgctgattgc gctggacgac gtgacggtgc gctacggcgc
      301 cgtgcacgcg ctgcagggcg tggtgctgcg catcgcgccg ggcgacagcg tggcgctgat
      361 cggcggcaat ggctcgggca aaagcacctt gctgcgcgtg gtgcacggcc tgatcgcgcc
      421 cagcagcggc cagtgccagg cgccgccgcg cgccgcgcag gccatgctgt ttcagcgccc
      481 gtggatgctg cgcgccagcg cgcgccacaa catcgccgtc ggcctgtggc tgcgcggcgc
      541 gccgtggcgc gcggcccagg cgcgcgcgct tcaggcgctg cagcgcgttc agctggccga
      601 tgtggccgat cgcggtgcgc ggggcctgtc gggcgggcag cagcagcggc tggcgctggc
      661 ccgcgcctgg gcgcagcaac cgcaactgct gctgctcgac gagccgaccg ccagcctcga
      721 cccgcacgcc aagcacgagg tcgaggcgct catgcaagag ttttccagcg cgccgacggc
      781 agacagcgaa gcggcgagcg caccgctgac catgctgttc gccagccaca acctgggcca
      841 ggtcaaacgg ctggcgcggc gcgtggttta cctggagcag gggcgcgtgc tggccgacct
      901 gccgacggag gatttcttca accgcgcgct gctgcgcagc gtgtcacccc aggccgattg
      961 gttcctcaaa ggagagttgt catgagtttt atcacgtctt tttggccgtt ggcgcacgcc
     1021 aggcaagcgc tggtagctat tggtttggta gcgttttcgg tgggcgcctc ggcgcagtcc
     1081 atcgtggtgg cgtccaccac atcgaccgag caatcgggct tgttcggcta cctgctgccc
     1141 gagttcaaga aggccaccgg cattgaggtc aaggtggtgg cgctgggcac cggccaagcg
     1201 ctggacacgg cgcggcgcgg cgatgccgac gcggtgttcg tgcacgacgt gcaggccgag
     1261 cagaagttcg tcgccgaggg ttttggcgtc aagcgctacc cggtgatgta caacgacttc
     1321 atcctggtcg ggccgaagaa cgacccggcc ggcgtgcgcg gcaaggacat cgtcgaaggg
     1381 ctgaagaagc tggccgccgc caacgccgcg ttcatctcgc gcggcgacaa gagcggcacg
     1441 cacgcggcgg aattgcgctt ctgggaccag gccggcctga aggacgccaa gggcagcggc
     1501 tacaaggaat gcggctgcgg catgggcccg gcgctcaaca tggcggcatc gaccggcgcc
     1561 tatgtgctgg ccgaccgcgg cacctggctc aacttcaaga accgcgccga tctggccatc
     1621 ctggtcgaag gcgacaagcg cctgttcaac cagtacggcg tgatggtggt caaccccgcc
     1681 aagcacccgc aggtgaaggc ggccgaggcg cagaagttcg tcgactggct ggtatcgccc
     1741 gcggggcagt cggtcatcgc cggctacaag atcggcggcg agcagctttt tttccccaac
     1801 gccgagaagt gacgtgggcg gcggcgggcg gtgttgctgg tcggcagcgc gcccgcgccg
     1861 tgcccgggtg gcccccgatc gtgaactttt taacggtgaa ccccccgtga accggggctt
     1921 gtgcacggcc ttgatgaata atttccgcct acttcggaga ctcatcatgc cgacccccac
     1981 atcgatcacg caatgcctgc tgggcctggg cttggcggcg gcgctgctgc cagccgcggc
     2041 gcagaccacg gtgctgatca acccgggcga ccagatcgaa ggcgatcggc tggccgtgtt
     2101 caacacctgg aagaacgcgc tggacgccag cttgcgcaag gtgggcgctg ccaacgtgca
     2161 aacgcgcttt tcaagcgacg ccacggctga tttgagcgcc acgcgcgcca acaccttcga
     2221 cgtcatcgtg gcacctgcgc cgaccatcgg cagcgctgtg cgccacggct aagtgccggt
     2281 gatgggcctg gccgacaccg cgcgcaccgt gctggtggcg ccggccgaca gccccatcag
     2341 cgacctggcc caaagccagg gcaaacgcgt cggcatgccg gggcaagaca gcgtggtgac
     2401 ctacctgctg cgcggcgaag tgcaggccaa caacatgacg ctgcgcaaga agtacgcggc
     2461 ggtctaccag acgcgctacc aggacgcgct gctgcaatgc ctgcagctgc gccgctgcga
     2521 cgtggtggcg gtcgagcaca gcgtggcgaa gcgctggatc gccgccggcg acaaggtcaa
     2581 gatcgtctgg caaagccgcg aggtaccggg ccagagcgtg gcggtgcgca agggcgccaa
     2641 cctgtcggtc gacgcgctgc gttcggcgct gatgaccgag ttgccgaaag ccagcgccgt
     2701 cagctacacc gatgcgcgca cgctcaagga ttttgactac gtctcgtcgc tcggctactt
     2761 cacgccgcgc gtgctggccg gcgccacatt ggtcgaagac ccggccatga tcgaaaccct
     2821 gatggccggc ggcgcgcagt acatcgacac gcgcacgcag gccgaattcg accgcggcca
     2881 tgtgccgggc gcgcgcctgg tgccctacgt cgagaaaagc gccaaggacc cggactacga
     2941 cgccgcgcac gaccagttca agccgcagga cctgggcgcc gacaagtcga aggcgctgat
     3001 ctttgcctgc aacggcgccg aatgctggaa gagcttcaag gccagccacg ccgccatgaa
     3061 ggccggctac agcaaggtct actggttccg caccggcttt ccggcctggc gcgcctcggg
     3121 ccgcaaggtg gatggcgacg cggccaaggt gggctgaaag cgcccttagt ccagcagcgc
     3181 cacgcctttc acctgcgcaa acacgcgctg ccccgccgac agccgcaacg cagctgccga
     3241 cttgcgcgtg acgcgcgcca gcagcgtgct gccggccaga tccagcgcca ccagcgtttg
     3301 cgccgggcca tcgtcggtca gcgaatgcac cgtggccggc aacacgttga ggatgctggt
     3361 gtcgctggcc ggcgccagcg ccaggctgac gtcgcgcgcc agcacgcgca ggcgcaggcg
     3421 ctcgcccacc tggcgcggcg ggctgccggc ggcctgaacg cagtgcagca cgccgccggc
     3481 aaagcgcacc tccagcaaat tccagcgctg gtcgatggcg gccagcgcac catcgatcac
     3541 ggcgcccgcg tggtcgccgt gcgccagcgg caggtcaagc cgcgtcagca actcgcccgt
     3601 ggggccgttg gcgctcacgc ggccggcgtc cagcaacacc atgtgcgtgg ccagccgtgc
     3661 cacttcgtcc agcgagtggc tgacgtagag caacgggatg cgcagctcgc gctgcaggcg
     3721 ctcgaagtag ggcagcagct cggccttgcg cgcggcatca agcgcggcca gcggctcgtc
     3781 catcagcagc aggcgcgggc tggtggccaa ggcgcgcgca atcgccacgc gctggcgttc
     3841 gccgcccgac agctgcgacg gccggcgtgc cagcaggtgg ccaatgccga gcagttcgat
     3901 ggcctgatcg agcgcaatgc gccgctctgc cgccggcacg cgcttcaggc cgaagtcgag
     3961 gttgccttgc accaacaggt gcgcaaacag gctggcctcc tgaaacacat agcccagcgc
     4021 gcgccggtgc gtgggcagcc agacgcccgc agcatcgtct tgccacatct ggtcgccgac
     4081 cacgacgcgc cctggctgcg ggcgcaccag cccggccacc gcgcgcagca gcgtggtctt
     4141 gccgcagccc gaggggccga acagcgccgt gatgccggcg cccggcagcg gcagatcgac
     4201 atcgagcgtg aacgcatccg gcccgctgcc gaacgccacg cgggcgcgca cctgcagtgc
     4261 ggccgcgttc atgcgcggcg cctcgtcggc ggttgcagcc agtgcagcgt cagcagcacc
     4321 ccaaagctga acaccagcat gccgccggcc agccagtgcg cctgcgtgta ttccagcgcc
     4381 tcgacgtggt cgtagatctg caccgaaatc acgcgcgtct ggccggggat gttgccgccg
     4441 atcatcagca ccacgccgaa ttcgcccagc gtgtgcgcaa agcccatcac ggcgcccgtc
     4501 aggtagcctg gccgcgccag cggcagcgcg acgtgccaga aggcatccag cggcccggcg
     4561 cgcaaggtgg cggcggcttc cagcgggcgc tcgccgacgg cttcaaacgc gttgatcagc
     4621 ggctgcacca caaagggcag cgaatacacc agcgagccga tcagcaagcc ccagaaggtg
     4681 aaggccagtg tgccccagcc gagcgactgc gtgatctgcc cgatggggcc gttggcgccc
     4741 agcgtgacca gcaggtaaaa gcccagcacc gagggcggca gcaccagcgg cagcgcgacc
     4801 agcgagcgaa tcggcagccg ccagcgcgag cgcccgcgcg ccagccacca ggccagcggc
     4861 gtgcccagca cgagcagcga cagcgtggtg acgaccgaca actcgatcga caaacggatg
     4921 gcggcaaggc tggtggcgtc gagcatgggt caggtgatgg cgcccgcgaa gaaccacggc
     4981 gcgctataca catgatagct gctcgcgctt gatgggcaag cgccagcggc ataaaacgct
     5041 ttgaatcgtg ggcgcgggcc tatttcacct cgtagccgta gctgcggatg aagtcctggg
     5101 tattgggtga acgcatcagc gccatccagg ccttggcggc cgggttggcc gcggcgcgtt
     5161 tcaagatcgc ggcgtcctgg atgatgggcg cgtggtactt ggccggcacc acccacatcg
     5221 agccgctttt catcttgccg ccatccagca cctgcgccag cgcgacaaag cccagctcgg
     5281 cattgccggt ggcgatgaag ttgtaggtct ggccgatgct ctcgcccgtc accagcttgg
     5341 gagtcagcgc cgccgccagg cccagcttgt ccatcgtctc gaccgccgcc gcgccgtagg
     5401 gtgcgagctt cgggttggcg atggcgatct tgttgaaatt gccgcctttc agcaccgcgc
     5461 cctcgccatc gacaaggccc ggtttggccg accacagcac cagctgcccg atcgtgtacg
     5521 taaagcgcga gcccggcacg gtgtcgcctt ctttttcgag ccgaatcgga cgctcgtcgt
     5581 cggccgcaag caggatgtcg aacggcgcgc cgttcttgat ctgggtgtag aacgcgccgg
     5641 tggcaccgaa ggacagcttg gcctcgtgcc cggtggtcct ggcgaagatc tcggccaacg
     5701 cgcgcgccgg cgcagtgaag ttggcggcca cggcgatctg taccacctcg gcctgcgcgg
     5761 cggcggaaag cgccagcgcg gcggtcaggg tcagcaggcg gggaaggttg aaacgcatga
     5821 acggagctcc tcgatcagtg aagcagcgct ttgcgtgctt cgctattcat aaatggaata
     5881 gcgagtgtag ccgtgttcgc tcagccaccg cgccacaatc gtggccatgg gcaagtcact
     5941 cgtttccgcc gccgcactcg gacacggccc ggcggacaag cgcatggaaa ttctgcacct
     6001 ggtgggccgg catggctcca tctcgcaggc ggcgcgcagc gccggcgtca gctacaaggc
     6061 ggcttggcag gccatccaca cgctcaccaa cctggccggc gagccgctgg tagacagcag
     6121 cgtgggcggc gccggcggcg gcggtgcgcg cctgacaccg gcgggcgagc gcctgctggc
     6181 cgccgccgaa cagcttgaca gcgcgcggcg ccgcgtgctg gcgcgcgtca acggtgcaga
     6241 aagcgcggcg ctcgccggcc cgcgcaccag catgcgcaac cacctgcgcg ccgtggtcga
     6301 gcggctggag agcgatggcg cgcgcgaccc catggtgcgc gtgctgctgg ggctggccgg
     6361 tggcggcacc ctggcctcga tcatcacccg cgaaagcgcc gagctactgg gcctggccgc
     6421 cggcttgccg gtgctggcgc tgtgcaaggc gacggcggtg cgcgtacacg ccgcagaggc
     6481 ggcggcgccc atgggaggca atcggctgcc cgggcgcgtg ctgcgcgtgt cgcgtggtgt
     6541 gcagcgcgat gaagtggtgg tcacgctggc gggcgatcag caactggtcg gctttgccgc
     6601 ccgcccgaac cggctgcgcg ccggcagccg caccgatgcc gtgctggacg acagcgcggt
     6661 ggtgctggcg ctgacgagct gaaggcgcct gccgtcagct gaattcgaac gaaaacaggc
     6721 gccagcgctt gtccagaaag cgcgagcagc tataaaaata gcagttcgat caacccgttg
     6781 cccacagcaa cggaaacagc gcgtcgtcca tcggcgcgcc ggccggcaag gcgctgatca
     6841 gttccagcat gaccaccctc gccgcgcacg ctggcttaga agatggcgtg ttcatgctca
     6901 aaaccctgcc tggcgcttgc ccatcaatcg ctggcagcta tgaaaaaacg agcggtctga
     6961 atgcccagcc aggtcagcgc cagcgagccg agcaggtgca gcgcggccgt cgacagcgcc
     7021 gcg
//
LOCUS       DAOMVO010000111         7022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2863_length_7022_cov_13.306158, whole genome shotgun sequence.
ACCESSION   DAOMVO010000111 DAOMVO010000000
VERSION     DAOMVO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2863_length_7022_cov_13.306158"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKV30_06925"
     CDS             complement(<1..445)
                     /locus_tag="PKV30_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRL29981.1"
                     /translation="MEISKAELKSAAAASAGSDTSELRGAEILVKALQAEGVKYVWGY
                     PGGAVLHIYDAFYKQDTIQHVLVRHEQAAVHAADGYARATGDVGVALVTSGPGVTNAV
                     TGIATAYMDSIPMVIITGQVPTAAIGLDAFQECDTVGITRPIVKHN"
     gene            736..1302
                     /locus_tag="PKV30_06930"
     CDS             736..1302
                     /locus_tag="PKV30_06930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRL29982.1"
                     /translation="MATEQELSDFLKSVEKRAFKRSVYHVRDEDAALDIVQDSMMKLA
                     EHYGDKPAAELPLLFQRILSNSTLDYFRRQKTRNAVFSNMSDFESPDGDGDFDLLEAF
                     AGASDSQQIESAEDSTRRAQILHEIELQIQALPNRQREAFLMRYWEEMDVAETAVAMG
                     CSEGSVKTHCSRAVQALAKALRARGILP"
     gene            1299..1760
                     /locus_tag="PKV30_06935"
     CDS             1299..1760
                     /locus_tag="PKV30_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HRL29983.1"
                     /translation="MNTNYRQLHTDNLQNRFGLRVAARLSAGTEGLPHDIGERLRVAR
                     EQAVARRKQPAAVLRQRAALAASRNGNTATLSFGDDGLGFWGRLTSAALVLVMAAGLI
                     AINVIQDDDRTMEVADLDAALLTDDLPPEAYADPGFLQYLKTGAQSAGTSR"
     gene            1801..2406
                     /locus_tag="PKV30_06940"
     CDS             1801..2406
                     /locus_tag="PKV30_06940"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HRL29984.1"
                     /translation="MLPALVLALSTWALAPAWAQGVGDGLPAVPAAGAIEWRSLSAEQ
                     RAALQPLEKSWRTLGADQQRKWVALTQNYHRMTPEERSTLQSRMTEWVNLTPAQRTQA
                     RLNFGEVRKVPADEKRAKWEEYQALPVEERERLANDRPKPPIGAAPALRPAPADRLLR
                     PVRAPVPAATPSAPVSATPPAPRVNRNTLLPQPPASASQPR"
     gene            2521..3015
                     /locus_tag="PKV30_06945"
     CDS             2521..3015
                     /locus_tag="PKV30_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRL29985.1"
                     /translation="MRAPSLRRRMACWLYEGTLLFGVVVLPGLIFGMLTNTRHALDNR
                     HGLQALIFGVLALYFGYFWTKGQTLPMKTWHIRMVDRAGQPVRWPRALLRYLLAWLWF
                     LPPLAAMAPFKLSTGEVTVLMLGWIVLWALLSRFQRDQQFWHDAWAGTRLIDVQPDLA
                     RRRQ"
     gene            3071..3493
                     /locus_tag="PKV30_06950"
     CDS             3071..3493
                     /locus_tag="PKV30_06950"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRL29986.1"
                     /translation="MNSTPPAHGTTAPEVVNPQKARTGFSRVWHATGYSIEGLKAGWG
                     EPAFRQEAIAAIVLLPVAFWLGRSWTETALLAGSVLFVMIVELLNTGIETAIDRIGPE
                     WHDLSKRAKDMGSAAVLLSLLLAGGIWLAALYTHFIQP"
     gene            3490..4086
                     /locus_tag="PKV30_06955"
     CDS             3490..4086
                     /locus_tag="PKV30_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRL29987.1"
                     /translation="MSPRIEPAFSICVYCGSRPGVDPAFAAVAAQVGHWIGRHGGQLV
                     YGGGGGGLMGIVADAALTASGRVVGVIPRALVELEHAHRGCTELIVVETMHERKRIMA
                     ERCDAFLALPGGIGTFEEFFETWTWRQLGYHDKPTGLLNQNGYYDALLGFLRHSVASG
                     FMGESQMALIEVDPDWQNLLQTLVRTAGFPSPTELEKI"
     gene            complement(4153..4362)
                     /locus_tag="PKV30_06960"
     CDS             complement(4153..4362)
                     /locus_tag="PKV30_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HRL29988.1"
                     /translation="MNEDTIAGNWKQFKGKVKEQWGKLTDDDLDVIAGKRDQMVGRMQ
                     ERYGIAKDEAEKQLSDWETRSNYRW"
     gene            complement(4541..5740)
                     /gene="hemW"
                     /locus_tag="PKV30_06965"
     CDS             complement(4541..5740)
                     /gene="hemW"
                     /locus_tag="PKV30_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRL29989.1"
                     /translation="MNDVQHYLRPGTLQLPALPPLSLYVHLPWCLKKCPYCDFNSHER
                     RAGVDAAAQQTRYVDALMADLEASLPLVWGRPVISVFIGGGTPSLFEPEAIDRLIAGV
                     RARLPLAPGCEITLEANPGTFERERFAAFRAAGVTRLSVGVQSFNDAHLKAIGRVHDG
                     AQARAALEEAARAFDTFNLDLMYALPGQSLAELDQDLRTALSFKPPHLSIYHLTVEPN
                     TVFAKFPPRLPDDDTAWDMLDRITETTAQVGLERYEVSAYARAGHRCLHNLNYWQFGD
                     YLGIGAGAHGKLSFPHRIVRQVRHRQPELYMERALAGDAVAQAHEVARADLPFEFMLN
                     ALRLKDGLSLLDYTEKTGMPPSSIGLALQKAEQSGWLACDGAGFKPTPRGFDFLSDLQ
                     QLFLPSP"
     gene            complement(5737..6336)
                     /gene="rdgB"
                     /locus_tag="PKV30_06970"
     CDS             complement(5737..6336)
                     /gene="rdgB"
                     /locus_tag="PKV30_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRL29990.1"
                     /translation="MKIVLASHNSGKLAELQAMLAPLGVELVRQGDLGIGEADEPFHT
                     FVENALAKARHAARASGLPALADDAGLCVDAFGGLPGVDTAFYATQFGYEKGDANNVR
                     ALLEQMQGIDNRRAALVSTLVALRHADDPEPLIAVGRVVGEIARERRGTGGFGFDPVM
                     FIPEFGQTFAELPPDVKNAHSHRGRAAAAMVELMRQRWL"
     gene            complement(6381..>7022)
                     /gene="rph"
                     /locus_tag="PKV30_06975"
     CDS             complement(6381..>7022)
                     /gene="rph"
                     /locus_tag="PKV30_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRL29991.1"
                     /translation="GSVLIEFGQTKVLCTASVEEKVPPHKRGSGEGWVTAEYGMLPRA
                     THTRGDREAARGKQSGRTQEIQRLIGRSLRAVFDLRKLGERTIHLDCDVIQADGGTRT
                     ASITGAFVAAQDAVNWLLQRGAIAESPILQPVAAISVGIVRGVPLLDLDYAEDSACDT
                     DMNVVMTGAGHFVEVQGTAEGAAFSRAEMDRLLQLAEKGIGELIELQRKSLSK"
BASE COUNT         1161 a         2352 c         2268 g         1241 t
ORIGIN      
        1 agttgtgctt gacgatgggg cgcgtgatgc ccacggtgtc gcattcctgg aaggcatcca
       61 gcccgatggc ggcggtcggc acctggccgg tgatgatcac catcgggatc gaatccatgt
      121 acgccgtggc gatgccggtg accgcgttgg tcacgccggg gccggaggtg accagcgcca
      181 cgcccacatc gccggtggcg cgcgcgtagc cgtcggcggc gtgcaccgct gcctgctcgt
      241 ggcgcaccag cacgtgctgg atggtgtcct gcttgtagaa agcgtcgtaa atgtgcagca
      301 ccgcgccgcc cgggtagccc cagacgtact tcacgccttc ggcctgcaag gccttgacga
      361 ggatttcggc accacggagt tcggatgtgt cgctgccggc ggatgcggct gcggccgact
      421 tgagctcggc tttggagatt tccattttta gatcaacctt tcgagaattt ctctgacgaa
      481 aaaccttggg tgccccttgc cagcccttgt ggagccgctt caggacttga gaccacgcgc
      541 catgggcgga cttgagatgc cttccgccgg acggtgaccc gtttgccttt ttgactgcgg
      601 tgcggattat cgcactgcaa catggcttgc cggcgtccgg cgcccaaatt cttcaggacc
      661 cgggtgccat aatcgcgccc aaatcgcccg ccgggccaca ccgtggccgg ccctcttccc
      721 gccgcaacag acctcttggc caccgaacag gaactctccg actttctcaa aagcgtcgag
      781 aaacgggcgt tcaagcgctc ggtctaccac gtacgcgacg aggacgcggc gctggacatc
      841 gtgcaggaca gcatgatgaa actggccgag cactacggcg acaagccggc ggccgagttg
      901 cccctgttgt ttcagcgcat cctgtcgaac agcacgctgg attacttccg gcgtcagaag
      961 acgcgcaacg ccgtgttctc gaacatgagc gacttcgaat cgcccgacgg cgacggtgat
     1021 ttcgacctgc tggaagcctt tgccggcgcc agcgattcgc agcaaatcga gagcgccgag
     1081 gattcgacgc ggcgcgccca gatcctgcac gagatcgaat tacagattca ggccctgccc
     1141 aaccgtcaac gggaggcgtt tctgatgcgt tactgggagg aaatggatgt tgccgaaacg
     1201 gcggtggcca tggggtgttc cgagggcagc gtcaagacgc actgctcccg cgcagtccag
     1261 gcgctggcca aggcattgcg agccagggga atcctgccat gaacacgaac taccgccagt
     1321 tgcacaccga taacctgcaa aaccgcttcg gcctgcgcgt ggccgcccgc ctgtctgccg
     1381 gtaccgaggg cttgccgcac gacatcggcg agcgcctgcg ggtggcgcgc gaacaggccg
     1441 tggcgcggcg caagcagccg gcggcggtgc tgcgccagcg cgccgccctg gcggcgtcgc
     1501 gcaatggcaa caccgccacg ctgagctttg gcgacgacgg actgggtttc tggggtcggc
     1561 tcacgtcggc ggcgctggtg ctggtcatgg cggccggctt gatcgccatc aatgtgatcc
     1621 aggacgacga ccgcaccatg gaggtggccg atctcgacgc tgccctgctg acggacgact
     1681 tgccgcccga ggcgtatgcc gaccccggct ttctgcaata cctgaaaacc ggcgcccaaa
     1741 gcgctggcac cagccgttaa gctgcggtgc cgatcgagtt tcccgtactg aatgcgctat
     1801 ttgctgccgg ccctcgtcct tgcgctgtcc acctgggcgc tggcaccggc gtgggcgcaa
     1861 ggtgttgggg atggcctgcc cgctgtaccc gcggcgggtg ccattgagtg gcgcagcctg
     1921 agtgccgagc agcgcgccgc cctgcaaccg ctcgaaaagt catggcgcac cctgggcgcc
     1981 gaccagcagc gcaagtgggt cgccctgacg caaaactacc accgcatgac gcctgaggag
     2041 cggtccacgc tgcagagccg catgaccgaa tgggtcaacc tgacgccagc ccagcgcacg
     2101 caggcgcggc tcaactttgg cgaggtgcgc aaggtgccag ccgacgagaa gcgcgccaag
     2161 tgggaggaat accaggccct gcctgtcgag gagcgcgagc gcctggccaa cgaccggccc
     2221 aagccaccga tcggcgccgc ccccgccctg cgcccggcgc cggccgatcg gctgctgcgc
     2281 cccgtgcgtg cccccgtgcc ggcggccacg cccagtgcgc cggtgagcgc cacgccgccc
     2341 gccccgcgcg tgaaccgcaa tacactgctg ccccagccgc cagcgtcagc gtcgcaacca
     2401 cgctgatcta gcgcacgctg cgccggcctt gtcctggcgc ggtaccttgg cagcgttttt
     2461 ttttgcatga ccccatccgt cacccactcg gctcctgacc ctgcggtcgc gcgcgcatcg
     2521 atgcgcgcac cgtcgctgcg ccgccgcatg gcgtgttggc tgtatgaggg caccttgttg
     2581 tttggcgtcg tcgtgctgcc cggtttgatc ttcggcatgc tcaccaacac ccggcacgcg
     2641 ctcgacaacc gccacgggtt gcaggccttg atcttcggcg tgctggcgct ctacttcggc
     2701 tatttctgga ccaagggtca gaccctgccc atgaaaacct ggcacatccg catggtggac
     2761 cgcgcaggcc agcccgtgcg ctggccgcgc gccttgctgc gttacctgct ggcgtggctg
     2821 tggtttctgc cgccgctggc cgccatggcg ccgttcaagc tgtcgacagg cgaggtcact
     2881 gtgctgatgc tgggctggat tgtgctgtgg gcgctgctca gccgattcca gcgcgaccag
     2941 cagttctggc acgacgcctg ggccggcacg cggctcatcg atgtgcagcc agacctggcg
     3001 cggcgccgcc aatgacggat caggcggcgt gcttcgccga aaagacaccg tcgtgctcga
     3061 caatcgacgc atgaattcga caccgccagc ccacggcacc accgcgccag aagtcgtcaa
     3121 cccacagaag gcccggacag gcttttcgcg cgtctggcac gccaccggct attccatcga
     3181 ggggctgaag gccggctggg gtgaaccggc gttccggcag gaagccattg ccgccatcgt
     3241 gctgctgccg gtcgcgttct ggctcggccg cagctggact gaaaccgcac tgctggccgg
     3301 ctcggtgctg ttcgtgatga ttgtggaact gctcaacacg ggcatcgaaa ccgccatcga
     3361 ccgcatcggg cccgaatggc acgacctctc caagcgcgcc aaggacatgg gatcggccgc
     3421 ggtgctgctg agcctgctgc tggccggcgg catctggctg gccgcgctct acacccactt
     3481 cattcagcca tgagcccacg cattgagccc gccttctcca tctgcgtcta ttgcggctct
     3541 cgccccggcg tcgatccggc gtttgcagcc gtggcggccc aggtgggcca ctggatcggc
     3601 cgccacggcg gtcaactggt gtacggcggc ggtggcggcg gcctgatggg catcgttgcc
     3661 gatgcggcgc tgacggccag cggccgggtg gtgggcgtca ttccgcgcgc gctggtcgaa
     3721 ctggagcacg cacaccgcgg ctgcaccgaa ttgatcgtgg tcgagacgat gcacgagcgc
     3781 aagcgcatca tggccgagcg ctgcgatgct ttccttgccc tgcccggcgg cattggcacg
     3841 tttgaggagt tcttcgagac ctggacctgg cgccagttgg gctaccacga caagccgacc
     3901 ggcctgctca accagaacgg ctactacgac gccctgctcg gctttttgcg ccacagcgtg
     3961 gcaagcggat tcatgggcga gtcacaaatg gcattgatcg aagtcgatcc cgattggcaa
     4021 aacctgctcc agaccctggt gcgaacggcg ggatttccgt ccccaaccga gctcgaaaaa
     4081 atctgaagtc tgatcgctgc acaagaaaaa gcgcgccgaa gttcggcgcg ctttttctca
     4141 agagtgggcg aattaccaac gatagttgga acgcgtttcc caatcggaca gttgcttttc
     4201 agcttcgtcc ttggcgatgc cgtagcgctc ttgcatgcgg ccgaccatct ggtcgcgctt
     4261 gccggcgatg acgtccaggt catcatcggt cagcttgccc cattgctcct tgaccttgcc
     4321 cttgaattgc ttccagttgc cagcgatggt gtcttcattc atggcgattg ctccttctat
     4381 tgagggttgt cacaagtgct tcgtgccttc acgaaatgga tgagtgcctt gcgatgactg
     4441 gactttagat tcgccgaagc gcgcacgcag taggtgcagg caggcgccgc gcgtcagcac
     4501 aggactaccc ggcgctgtgt ctttcgacgc tgcaaaagat ctacggcgag ggcaggaaca
     4561 actgctgcag gtcgctcaag aagtcaaagc cgcgcggcgt tggcttgaac cccgcgccgt
     4621 cgcacgcgag ccaacccgat tgctctgctt tttgaagcgc caggccaatg ctgctgggcg
     4681 gcatgccggt tttttcggtg taatcgagca ggctgaggcc gtccttcaac cgcagcgcgt
     4741 tgagcatgaa ttcaaaaggt aagtcggcgc gcgccacttc gtgcgcttgc gccacggcat
     4801 cacccgccag ggcacgctcc atgtacagct cgggctggcg gtgccgcacc tgccgcacga
     4861 tgcggtgcgg aaagctcagc ttgccgtgcg cgccggcgcc aatgcccaga tagtcgccaa
     4921 actgccagta gttgaggttg tgcaagcagc gatgcccggc gcgcgcatag gccgagacct
     4981 catagcgctc cagccccacc tgcgccgtcg tctccgtgat gcggtccagc atgtcccacg
     5041 cggtgtcgtc gtccggcagg cgcggcggga acttggcgaa caccgtgttc ggctccacgg
     5101 tcaggtgata gatcgacagg tgcggcggct tgaacgacag cgcggtgcgc aagtcttgat
     5161 ccagttcggc cagcgactgc cccggcaacg cgtacatcag gtcaaggttg aaggtgtcga
     5221 aggcgcgcgc ggcttcttcc agcgccgccc gcgcctgcgc gccgtcgtgc acgcgaccga
     5281 tggccttcag gtgcgcatcg ttgaagctct gcacacccac cgacaggcgc gtgacgcccg
     5341 ccgcgcgaaa ggccgcgaag cgctcgcgct caaaggtgcc ggggttggcc tccagcgtga
     5401 tttcgcaccc tggcgccagc ggcaggcgcg cccgcacgcc ggcgatcagg cggtcaatcg
     5461 cttctggttc aaacaggctc ggcgtgccgc cgccgatgaa cacgctgatc acgggccggc
     5521 cccagaccag cggcaagctg gcttccagat cggccatcaa tgcatccacg tagcgggtct
     5581 gctgggcagc cgcatcgaca cccgcgcgcc gctcgtgcga gttgaaatcg cagtacgggc
     5641 actttttcag gcaccacggc aggtgcacgt acagcgacag cggtggcagc gcgggcaact
     5701 gcagcgtgcc ggggcgcagg tagtgctgaa cgtcgttcac agccagcgct gacgcatcag
     5761 ttcgaccatg gcggcagcgg cgcggccgcg atggctgtgg gcgttcttca cgtcgggcgg
     5821 cagttcggca aaggtctggc cgaactcggg gatgaacatc acggggtcga acccgaagcc
     5881 gccggtgccg cgccgctcgc gcgcaatctc gccgacgacg cggccgacgg cgatcagggg
     5941 ctcagggtca tccgcgtggc gcagcgcgac caatgtgctg accagggcgg cgcgccggtt
     6001 gtcgatgccc tgcatctgct ccagcagcgc gcgcacgttg ttggcgtcgc ctttctcgta
     6061 accgaactgc gtggcgtaga aggccgtgtc cacgccgggc agcccaccga aggcgtcgac
     6121 acacaaacca gcgtcgtcgg ccagcgccgg caagccgctg gcgcgcgccg catggcgcgc
     6181 cttggccagc gcgttttcga caaaggtgtg aaacggctcg tcggcctcac cgatgcccag
     6241 gtcgccctgc cgcaccagtt ccacgcccag cggcgccagc atggcctgca gctcagccaa
     6301 cttgccgctg ttgtgcgatg ccaggacgat tttcatgtca aatatggctc cagcgcttgc
     6361 ctatcaagcg caagcagcta ctatttagat agtgatttgc gctgtagctc gatcaactcg
     6421 ccgatgcctt tttcggccag ttgcagcaag cgatccatct cggcgcgcga aaatgccgcg
     6481 ccttcggccg tgccctgcac ttccacgaaa tgaccggcgc cggtcatcac cacgttcatg
     6541 tcggtgtcgc aggccgagtc ttcggcgtag tccagatcga gcagcggcac gccgcgcacg
     6601 atgccgaccg agatggcggc caccggctgc agaatcggcg attcggcgat cgcgccgcgc
     6661 tgcagcagcc agttgacggc atcctgcgcc gccacgaacg cgcccgtgat gctggcggtg
     6721 cgcgtgccgc cgtcggcctg gatgacgtcg cagtcgaggt gaatggtgcg ttcgcccaac
     6781 ttgcgcaggt cgaacacggc gcgcaggctg cggccgatca ggcgctggat ctcctgcgtg
     6841 cgcccgctct gcttgccgcg cgcggcctcg cggtcgccgc gcgtgtgggt ggcgcggggc
     6901 agcatgccgt attcggccgt cacccaacct tcgccgctgc cgcgcttgtg cggcggcact
     6961 ttttcttcca ccgacgcggt gcacagcacc ttggtctggc cgaactcgat cagcaccgag
     7021 cc
//
LOCUS       DAOMVO010000112         6918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2931_length_6918_cov_12.218126, whole genome shotgun sequence.
ACCESSION   DAOMVO010000112 DAOMVO010000000
VERSION     DAOMVO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2931_length_6918_cov_12.218126"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKV30_06980"
     CDS             complement(<1..1036)
                     /locus_tag="PKV30_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29992.1"
                     /translation="MRRLSRGCCRIIVFIFFVIIHRKRDRLLAQLLDQHAAKLRQVAR
                     HVLGADEFINDLDRWCLWLVDAPPALLRSSSLVEGRLKAVRAYRSASKRDRTRQLAAT
                     PRFFGEIRQPDARYVLIPRHSSERREFVPMGYLDPDVICGDAKMCVPTATLLHFGILT
                     SSMHNAWVRAVCGRLKSDFRYSAAIVYNNFPWRFWQQDSAANQPPESVDQARTAIESA
                     AQGVLGARAQFPGSSLADLYDPLTMPPALLKAHQKLDAAVDKAYQLAGGPGATPTTPS
                     AWLFCSRSTNASPACCRPRARQADEARACASRRRRRDGVARQRGARASARAAAASRTP
                     GRCRLAARRGL"
     gene            complement(1061..1447)
                     /locus_tag="PKV30_06985"
     CDS             complement(1061..1447)
                     /locus_tag="PKV30_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL29993.1"
                     /translation="MSEPSALTAADRAFGLACPHCGLRAMSAPRKWVTGWGSVRACQS
                     CGQRVEVDGLRVMAVTAPFILGCLALAVWAGVGGRFSPVLALWCLSALALLGFLGYVL
                     AVPLRRVGRTDPQAVAQARRLAGEQG"
     gene            complement(1540..4632)
                     /locus_tag="PKV30_06990"
     CDS             complement(1540..4632)
                     /locus_tag="PKV30_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRL29994.1"
                     /translation="MALPVSDAPVRRPYTPPPPPPPPAPRKEVDPAVRQPALHQGKRD
                     APPPERYVQHEVERGENLTEISRRYQTTVPMLEAANPQIKNVDAIEIGQKVNVPIGAD
                     YGREPTRDVVEPGQTLTDMARQHAGVSAQDIARANRHEIPNADRIHAGQEVWVPADRP
                     ATPLDQKVQATDAAVAKVDRAQQAYDTLEHGTNRAIRDEMYEGIQSAKGNLKTAVQAE
                     VDERVKTIQPAGGKPTEADYAAAGNQVKDRYQSDPAATQKLDTALKSLADDRYRASPQ
                     GQAEAIVSKARGAGDAPKQVASLNDSLKTAPPKVRDAVMNSAQGKQLIQDAAQWATDP
                     LAGGDALKADENARQEFGGAGQQVPGGKTMERLEQLTRDLDPALAAQLTSQAMPTVEK
                     YLGEFQAQFGGQPLGPSGMANMLKVLDRGVDTPAGQANIERMAKLGVYDRDALRLHVS
                     GGGSPAYAIALSRQPGIDSATVLQQAYDGVAAYRAQITETAKDYSVHMEELGWLVQNH
                     GASMAPEQLQKAIADYTAEKNKSEPGWEATGKALQDKLAEQGATLQTQLDALRALPAG
                     TPGRDDAIAGALNDPAVGLALNTAWEKKPELLQGAKGDGLLGFFADPTVKGSAKLSDT
                     GRKLFTDVATQYMKHNVAGALETFNPADPASVAATREAVGTLRNSRLAAVLGVSPKEM
                     DAAVQALETAMPEAGDSADDVARKMSTLNGELDEIGEAVRGRLGFDPADAHRGMKAFD
                     KSTVAGQAIRGLGMLLGGVGLLASTGTAMSDPSLKNWLKAAGDGLGLTQKGLELAVGM
                     GKINEGSLLGKAGASLVGRVLGAGMAVFDVWSAVESAQQGDYTSAGLYGAGAVGGVTA
                     ALFTGPIGWAGLAVVGISAAGLWVWNGVKESRKHEPDSDGGTAMRFLQHAGLNESAAR
                     ALTDQSGDGHSVMPLLNRYAELKGMKLEDPAQQKKFIDWINGMQPEHLAALRDNLHET
                     ADDIDGDPAKFNATADGDRYVVPDIADRPWFTRMGDHGPESVAQLEAQLQVLQIRVLG
                     "
     gene            complement(4916..5950)
                     /gene="bioB"
                     /locus_tag="PKV30_06995"
     CDS             complement(4916..5950)
                     /gene="bioB"
                     /locus_tag="PKV30_06995"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRL29995.1"
                     /translation="MTLATTAEQPITLHKRPAPNTGADRWSVEQIEALLNLPFNDLLW
                     RAQQVHRQHFDANEVELATLLSVKTGGCPEDCGYCPQSVHYDTGVKAEKLMESGAVKK
                     AAEAAKAAGATRFCMGAAWRAPKDRDIDAVAELVRAVKDVGLEACVTLGMLADGQAET
                     LQRAGLDYYNHNLDCAPDFYGEVISTRDYQDRLDTLDRVRGAGVKVCSGGIVGMGESR
                     RQRAGLIAQLANLPEVPESVPINHLVKVEGTPLAEQPDLDPFEFVRMIAAARITMPTA
                     RVRLSAGRQQMGEAVQALCLLAGANSIFYGEKLLTTGNPDVSADRTLLTRLGMRARAG
                     VPGVAGFAEI"
     gene            complement(5982..6509)
                     /locus_tag="PKV30_07000"
     CDS             complement(5982..6509)
                     /locus_tag="PKV30_07000"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRL29996.1"
                     /translation="MLIEHHYPTPLGDMVALFTPPGLALLEFSENTKRLDREWRDVER
                     YAGAAAELGADARTQALGAEIDAYFKGLLQDFDTQLDLVGTPFQKSVWQGLLAIPYGQ
                     TRSYGQQAHQINHPTATRAVAAANGQNKIAVIVPCHRVIGSDGRLTGYGGGLPRKEWL
                     LQLERSGAVPRDLFP"
     gene            complement(6512..>6918)
                     /locus_tag="PKV30_07005"
     CDS             complement(6512..>6918)
                     /locus_tag="PKV30_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRL29997.1"
                     /translation="FMKVPTARFGEHRMFEQIGLPIEGLGDGFSWKSKDLEHSGQLAR
                     DGWQKAKAAILDGEHFLVVLDEITYPLMYGWLPLNDVLQTLEQRPRDVHVCLTGRRCP
                     QELIDIADTVTEMTLVKHAFKAGVPAQRGIED"
BASE COUNT         1024 a         2297 c         2374 g         1223 t
ORIGIN      
        1 aaaggccgcg gcgcgccgca agccggcatc ggcccggcgt ccgtgatgcg gccgcagcgc
       61 gtgccgaagc ccgcgcgccg cgctgccgag ctacgccgtc acgccgccgc cttcggcttg
      121 cgcaggcgcg cgcttcgtcg gcctgccgcg ccctcggccg gcagcaggct ggtgatgcgt
      181 tggtagagcg tgaacagaaa agccacgcgc tcggcgtcgt tggcgtagct cctgggccgc
      241 cggccagttg gtaggccttg tccacggcgg cgtcgagctt ttgatgggcc ttgagcagcg
      301 cgggcggcat ggtcagcggg tcgtataggt cggccaggct gctgccgggg aactgggcac
      361 gagcgccgag cacgccttgt gctgcggatt cgatagctgt tcgcgcttga tccacggact
      421 ctggaggctg gtttgctgct gaatcctgct gccagaagcg ccaggggaag ttgttgtaaa
      481 cgatggcggc ggagtagcgg aaatcgctct tcaaccgacc gcagacggcg cgcacccagg
      541 cgttgtgcat ggaactggta aggatgccga agtggagaag cgtcgctgtc ggtacgcaca
      601 ttttggcgtc accgcagatg acgtccgggt cgagatatcc catcggaaca aattcgcgtc
      661 gctctgatga atggcgcgga atcagtacgt atcgcgcatc gggttgacgg atctcgccaa
      721 aaaaacgggg tgtcgccgca agttggcgcg tgcggtcacg cttgcttgcg ctccgatagg
      781 cgcgaactgc tttcaggcgc ccctcgacaa ggctgctgct gcgcagtaac gcgggcggag
      841 cgtcgaccag ccataaacac caccgatcca agtcattgat gaactcgtca gcgccgagga
      901 catggcgcgc aacctggcgc agtttcgcgg catgctggtc gaggagctga gccagcagcc
      961 tatcgcgttt gcgatggatg atgacgaaga agatgaagac gatgatgcgg cagcagccgc
     1021 gcgatagccg gcgcacccga caagcgacgc gcggcgcctg ctagccctgt tcgccagcca
     1081 gccgccgcgc ctgagcgacc gcttgtggat cggtccgccc gacgcggcgc agcggcacag
     1141 ccagcacata ccccagaaag cccaacaatg ccagtgcact caggcaccaa agcgccagca
     1201 caggcgaaaa ccggccgccg acgcccgccc acacggccag cgccaggcag cccagaatga
     1261 aaggcgcggt gacagccatg acgcgcaggc cgtcgacctc gacgcgctga ccgcacgact
     1321 ggcaagcgcg cacgctgccc cagccggtga cccatttgcg cggcgcactc atggcgcgca
     1381 agccgcaatg cgggcaggcc aggccgaacg cacgatcggc agcggtcagc gcagacggct
     1441 cagacatgcg gcggtcctgt gacggcacgc ggcgcggcct gacggcggtg gtgaagctgc
     1501 gcgtggcgca tcatgttcgg tattgtcggc gctgcgcgct caccccagca cccggatctg
     1561 cagcacttgc aactgtgctt cgagctgagc gacggactcc ggcccgtgat cacccatgcg
     1621 ggtgaaccac gggcggtcgg cgatgtcggg cacgacgtag cgatcgccgt cggccgtcgc
     1681 gttgaatttc gccggatcgc catcgatgtc gtcggcggtt tcgtgcaggt tgtcgcgcag
     1741 cgcggccaga tgctcgggct gcatgccgtt gatccagtcg atgaattttt tctgctgcgc
     1801 cgggtcttcc agcttcatgc ctttcagctc ggcatagcgg ttcaacaagg gcatcacgct
     1861 gtggccgtcg ccgctctggt cggtcagcgc gcgagcggcg ctctcgttga ggccggcatg
     1921 ctgcaggaag cgcatggcgg tgccgccatc gctgtcgggc tcgtgtttgc gcgactcctt
     1981 gacgccgttc cagacccaga gcccggccgc cgaaatgccg accaccgcca gccccgccca
     2041 accgatgggc ccggtgaaca gggccgcagt gacaccgccc acggcgccgg caccgtacag
     2101 accggcactg gtgtagtcgc cctgctgcgc tgactcgacg gccgaccaca catcgaacac
     2161 cgccatgccg gcgcccagca cgcgccccac cagcgaggcg ccggccttgc caagcagcga
     2221 gccctcgttg atcttgccca tgccaacggc cagttccaac cctttttgtg tcaggcccag
     2281 gccatcgccg gccgccttga gccagttctt gagcgacggg tcgctcatcg cggtgcccgt
     2341 agaggccagc aggcccacgc cgcccagcag catgcccagg ccccggatgg cctggccggc
     2401 gacggtactc ttgtcgaagg ccttcatgcc acggtgggcg tcggccgggt caaagcccag
     2461 gcgcccgcgc accgcctcac cgatctcgtc caactcgccg ttgagcgtgc tcatcttgcg
     2521 cgcgacgtcg tcggccgaat cgccggcctc gggcatggcg gtttccagcg cctgaacggc
     2581 agcgtccatc tccttgggcg acacgccgag caccgccgcc agccgtgaat tgcgcagcgt
     2641 gccaacggct tcacgcgtgg cggcgacgct ggccggatcg gcggggttga acgtctccag
     2701 cgcaccggcg acgttgtgct tcatgtactg cgtggcgacg tcggtgaaca gcttgcggcc
     2761 ggtgtcgctc agcttggccg aacccttgac ggtggggtcg gcaaaaaagc ccagcagccc
     2821 gtcgcccttg gcgccttgca gcagctcggg ctttttctcc caggcggtgt tgagcgccag
     2881 gcccacggcc gggtcgttca acgcccccgc gatggcgtcg tcgcgccccg gcgtgccggc
     2941 cggcagcgcg cgcagcgcgt cgagctgcgt ctgcagcgtg gcgccctgct cggccagctt
     3001 gtcctgcagc gccttgcccg tggcttccca cccgggttcg ctcttgttct tttcagcggt
     3061 gtagtcggca atcgcctttt gcaactgctc cggcgccatc gaagcgccgt ggttctggac
     3121 cagccagccg agctcttcca tgtggacgct gtagtccttg gcggtctcgg tgatctgggc
     3181 gcggtaggcg gcgacgccgt cgtaggcctg ctgcagcacg gtcgcggaat cgatgcccgg
     3241 ctgccgggac agcgcaatgg cataggccgg gctgccgccg cccgacacgt gcaggcgcag
     3301 cgcatcgcgg tcgtacacgc ccagcttggc catgcgctcg atgttggcct ggccggccgg
     3361 cgtgtcgacg ccgcggtcaa gcaccttcag catgttggcc atgcccgaag ggccgagggg
     3421 ctggccgccg aactgcgcct ggaactcgcc caggtacttt tcaacggtag gcatggcctg
     3481 gctggtcagc tgcgcggcca gtgcggggtc aaggtcgcgc gtcagctgct ccagccgctc
     3541 catggtcttg ccgccgggca cttgctggcc ggcgccgccg aactcctggc gtgcgttctc
     3601 atcggccttg agggcgtcac cgcctgccag cgggtcggtg gcccattgag cagcatcctg
     3661 tatcagctgc ttgccttggg cgctgttcat cacggcgtcg cgcactttgg gcggggcggt
     3721 tttcaagctg tcgttgagcg aggcgacctg tttgggcgca tcgccggcgc cgcgggcctt
     3781 cgacacgatg gcctcggcct ggccctgcgg gctggcgcgg tagcggtcgt cggccaggct
     3841 cttgagggcc gtgtcgagct tttgcgtcgc cgccggatcg gactggtagc ggtccttgac
     3901 ctggttgccg gcggcggcgt agtcggcctc ggtgggcttg ccacccgcag gctggatcgt
     3961 cttcacacgc tcgtcgacct cggcctgcac ggcggttttc aggttgccct tggcgctctg
     4021 gatgccctcg tacatctcgt cgcgaatcgc gcggttggtg ccgtgctcca gcgtgtcgta
     4081 ggcctgctgc gcgcggtcga ctttggcaac ggcggcgtca gtggcctgca ctttttggtc
     4141 cagcggcgtc gccggacgat cggccggcac ccagacttcc tgcccggcgt gaatgcggtc
     4201 ggcgttggga atttcatgcc ggttggcgcg cgcgatgtcc tgcgccgaca cgccggcgtg
     4261 ttggcgcgcc atgtcggtca gcgtctgccc gggttcgacc acgtcgcgcg tcggctcgcg
     4321 gccgtagtcg gcgccgatgg gcacgttgac cttctggcca atttcgatgg cgtcaacgtt
     4381 cttgatctgc gggttggccg cttcgagcat cggcaccgtg gtctggtagc ggcgcgagat
     4441 ttcggtcagg ttctcgccgc gctccacctc gtgctgcaca tagcgctcgg gcggcggtgc
     4501 atcgcgcttg ccctggtgca gggcaggctg gcgcaccgcc ggatcgacct ccttgcgcgg
     4561 cgccggcggt ggcggcggag ggggtggggt gtatggacga cggacgggag cgtcggaaac
     4621 cgggagcgcc atggtgtgcc ttcaaggtgg aagcacgtgc gccgccaact gcaccgcacg
     4681 cgccgacatg cggcccctgg ggccagaaac cgcgtcaacg gcaggcagtc taaaaaagtg
     4741 agcaatgcac ggatagttgg tgcggttacc gttggtattc gcaccagcgc gcgcgtcatc
     4801 aagatgtcac gcagccgtgg atcgaagcgc aggcaaacgc gccatacagc gcttcgactg
     4861 ctataccaag catagctgct cgcgctctat tgacgcgcgc tgaagcgcaa aagacttaaa
     4921 tctcagcgaa cccagcaacc cccggaacac cggcacgggc ccgcatgccc aggcgcgtca
     4981 gcagtgtgcg gtcagcgctg acgtcggggt tgccggtggt cagcagcttc tcgccataga
     5041 agatgctgtt ggcgccggcc agcaggcaca gcgcctgcac tgcctcaccc atctgctggc
     5101 gacccgcgga caggcgcacg cgtgcggtgg gcatggtgat gcgcgcggcg gcgatcatgc
     5161 gcacgaactc gaacgggtcc agatcaggct gctcggccag cggcgtgcct tcgaccttga
     5221 ccaggtggtt gatgggcacg ctttcgggca cctcaggcag gttggccagt tgcgcgatca
     5281 ggccggcgcg ctggcggcgc gattcgccca tgccgacgat gccgcccgag cacaccttga
     5341 cgccggcgcc acgcacgcga tccagcgtgt cgagccgatc ctggtagtcg cgcgtgctga
     5401 tgacctcgcc gtagaaatcg ggcgcgcagt cgaggttgtg gttgtagtag tcgagcccgg
     5461 cacgctgcag cgtctcggcc tggccgtcgg ccagcatgcc gagggtgacg caggcctcca
     5521 gccccacgtc cttcaccgcg cgcaccagct cggccacggc atcgatgtcg cggtccttag
     5581 gcgcgcgcca ggcggcgccc atgcaaaagc gtgtggcgcc ggcggccttg gcggcttcgg
     5641 cggctttttt aacggcgccg ctttccatca gcttctcggc cttgacgccg gtgtcgtagt
     5701 gcaccgactg cgggcagtag ccgcagtcct cggggcagcc gccggtcttg acggacaaca
     5761 gcgtggccag ttcgacctcg ttggcgtcga agtgctggcg gtgcacttgt tgtgcgcgcc
     5821 acagcaggtc gttgaagggc agattgagca gcgcctcgat ctgctcgacc gaccagcggt
     5881 cggcgccagt gttgggcgcg ggccgcttgt gcagggtgat gggttgttcg gcggtcgtgg
     5941 ccaatgtcat gttggtggct ttccttgtcg tgtggtgggg ttcaggggaa caggtcgcgc
     6001 ggcacggcgc cgctgcgctc cagttgcaac agccattcct tgcgcggcag gcccccgccg
     6061 tagccggtca ggcgcccgtc gctgccgatg acgcggtggc acggcacgat gacggcgatc
     6121 ttgttctggc cgttggcggc ggccacggcg cgtgtggctg tggggtggtt gatctggtgc
     6181 gcctgctggc cgtagctgcg cgtctggcca tacgggatcg ccagcagccc ctgccacacg
     6241 ctcttctgaa acggcgtgcc gaccagatca agctgggtgt cgaagtcctg gagcaggcct
     6301 ttgaagtacg cgtcgatctc cgcgcccagg gcctgcgtgc gcgcatcggc gcccagctcg
     6361 gccgccgcgc cggcgtaacg ctcgacgtcg cgccattcgc ggtccagccg cttcgtgttt
     6421 tcgctgaact ccagcagcgc cagacctggc ggcgtgaaca gcgccaccat gtcgccgagc
     6481 ggcgtggggt aatggtgttc gatgagcacg attagtcctc gatgccgcgc tgtgctggca
     6541 cgccggcctt gaaggcgtgc ttgaccagcg tcatctcagt cacggtatcg gcgatgtcga
     6601 tcagctcttg cgggcagcgc cggccggtga ggcacacgtg gacatcgcgc gggcgttgct
     6661 ccaaagtttg cagcacgtca ttcagcggga gccagccgta catgagcgga taggtgattt
     6721 cgtccagcac caccagaaag tgctcgccat ccaggatggc cgccttggcc ttttgccagc
     6781 cgtcgcgcgc gagttggccg gaatgctcca ggtccttgct cttccagctg aaaccgtcgc
     6841 ccagcccttc gatgggcaga ccgatctgct cgaacatgcg gtgctcgcca aagcgcgccg
     6901 tgggcacttt catgaact
//
LOCUS       DAOMVO010000113         6916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2932_length_6916_cov_16.150561, whole genome shotgun sequence.
ACCESSION   DAOMVO010000113 DAOMVO010000000
VERSION     DAOMVO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_6916_cov_16.150561"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2441)
                     /locus_tag="PKV30_07010"
     CDS             complement(<1..2441)
                     /locus_tag="PKV30_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL29998.1"
                     /translation="MFKWLLDNSLTNRLLVIIASVVLMAYGAFTLSRTPVDVFPDLNK
                     PTVTIMTEAGGMAAEEVEQLITFPLETTMNGLPGVESVRSTSSAGLSFLYVTFDWSTD
                     IFRARQLVSERLSSMEEGIAEGVVPRMGPISSIMGEIMQIAIPVDPSKISPMAVREYA
                     DWVLRPRLLSVPGVAQVIPIGGEVRQFQVQPNTARMAELGITHDQLESALKGYSSNTS
                     GGFLELNGREYLIRNLGRTSRLDDLKNLALAAKGGQPILLRQIAEVTFAAALKRGDAG
                     FEGKPAVILGIQKQPTADTIALTKSIEDALVGLKASLPAGMEAPRVTFRQASFIEASI
                     TTLQGKLIGASIFVAVILFFFLGTLRPTVIALTAIPVSIFITALVFKYFGLSINTMTL
                     GGLAIAIGGLVDDAVVGVENVLRRLKADRANHPHSRLHPIEIVAHATMEVRSAILYAT
                     VIIVLVFIPLFALPGLEGKLFVPLGIAFIVSTLASLIVSVTVTPVLSFYLLPRMKNLD
                     HGDTKVLAWLKARYRSGLQGVLDRPKAAMAAAGAAVLVAAVAVPFFPTTFLPPFNEGT
                     LLIGLRLNPGVTLTESSALARQAEVLVKQVPEVTHVGRRSGRAELDEHAEGVHVSELD
                     VGLKPTAELTRSMDEIKADIRKRLINLPAALEIGQPISHRIDHMLSGVRSQIAIKIFG
                     EDLDALRGQADALRAKLATIPGIADLQIEKQVLAPQIKVRIDYAAAAQYGVPAPQVLS
                     ALQALVEGEKVTQIVEGGRRFALVVKLPESARSVEGLGQILIETPNGRIPLSKIAAIE
                     DGDGPNQISRDDGKRR"
     gene            complement(2443..3561)
                     /locus_tag="PKV30_07015"
     CDS             complement(2443..3561)
                     /locus_tag="PKV30_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRL29999.1"
                     /translation="MKRASLHRLAWLGVVLALVGPLTAQAGEGHDHGDAPAAPSANGP
                     QRQPDGSVFLPKPAQRQLGVRTMVAAEAELPRSVELNGKVLMDPNAGGKVQPLNAGRI
                     EPGPRGLPNPGQVVRKGEVLAYVVPSTAPIERSNQSAQLAELRAAKSLADKRVTRLKE
                     LSDTVPRKDIEAAESEAASLAERMAAVGGGLATREALVAPVSGVIASAHVVAGQVVDA
                     RELLFEIIDPSRLRIEALAFEPSLAANVGSATLAVGDQRVPLEYLGAARSLREQALPL
                     GFRAQGAALNGLAVGQPVRVFVQTKQKVKGIAVPVASLMKNPANQTVVWVKTSPERFE
                     PRSITTEALDGVNVAVTSGLKAGDRIATQGATLINQVR"
     gene            complement(3558..4118)
                     /locus_tag="PKV30_07020"
     CDS             complement(3558..4118)
                     /locus_tag="PKV30_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30000.1"
                     /translation="MKSSHSLAALCLASVLLGNALPVIAGDGHDHGDTAPAATGTALP
                     RFAAVSETFELVGVLDGKQVTLYLDRFVDNTPVRGAQIELEIDGTKFKAEAHGDDAYE
                     VVLKEAPKPGVLPITATVTAGAEVDLLAGELDLHEAAHTDEPAHELSWKEVGGWASGG
                     LAVLAVLVFGGRRLMAARQVRAGGAA"
     gene            complement(4177..5484)
                     /locus_tag="PKV30_07025"
     CDS             complement(4177..5484)
                     /locus_tag="PKV30_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRL30001.1"
                     /translation="MPAGRWHHAARATLATVASTALTAALMGVAIEARAQTPAAPSEP
                     TPPTLRAAFETAWARQPEAQALAARRDAARAQRDAAQAWTPEPVAMELSTKTDRLNRN
                     LGTREVEVGVAIPLWLPGERGRSQALADAEGRAIESRTTAAQLRVAAAVREAWWAWQR
                     ARVELDAARGQLDNVQRIAADVGKRLRAGDLARADQHQADGAVAAAEAAVAQAEGALT
                     AAWQQLRPLVAMPAAAAPSAAPGADAEPEPSTATGPADGLDTHAELLSLKDRSAVADG
                     VAALAATQSRASPELTIAATRDRGTYGESYQQTFTVGVRIPFGAGPRHDARVASARAE
                     AVELQAQMALERARLAGEREAARARTDAARAQLAAADRRAQLARESRGFFDKSFRLGE
                     SDLPTRLRIEAEAADAERQAVRARIELAAAISAWRQALGLLPQ"
     gene            complement(5667..6029)
                     /locus_tag="PKV30_07030"
     CDS             complement(5667..6029)
                     /locus_tag="PKV30_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30002.1"
                     /translation="MRRWLAILMLALLPLQFSWAAVAAYCGHETGQHVQHLGHHEHQH
                     TDEATADQGSAPADQSAPAGFDFDCGHCHGTCASMPAPMGDISPLALASHPVTPVEGT
                     VRTLAQSPPERPQWLPLA"
     gene            complement(6093..6803)
                     /locus_tag="PKV30_07035"
     CDS             complement(6093..6803)
                     /locus_tag="PKV30_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRL30003.1"
                     /translation="MEAKAQLLQPTRLLDFTHPAIEALVEKRGWRPLPMYDRIGAVYD
                     FVRNEIAFGYNAGDELPASAVLADGIGQCNTKGTLLMALLRAVGIACRFHGFTIDKPL
                     QKGAITGLAYVLAPRRIIHSWVEVAFEGRWVNLEGFILDAPYLTSLQRRFPVRQRFCG
                     YGAATPDLSAPGVEWRGQDTYIQKDGIADDFGVFDSPDEFYARHGSNLSGLKRWLFTQ
                     VIRHRMNAQVRRIRAEKW"
BASE COUNT         1049 a         2312 c         2442 g         1113 t
ORIGIN      
        1 cgccgcttgc cgtcgtcacg gctgatctgg ttggggccgt cgccgtcctc gatcgccgca
       61 atcttcgaca gcgggatgcg cccgttcggc gtctcgatca ggatctggcc caggccttcg
      121 accgaacgcg ccgactcggg cagcttgacc acgagtgcga agcgccggcc tccctcgacg
      181 atctgggtga ccttctcccc ctcgaccagc gcctgcaatg cggagagcac ctgcggcgcc
      241 ggcacgccgt actgcgccgc ggcggcgtag tcgatgcgca ccttgatctg cggcgccagc
      301 acctgcttct cgatctgcag gtcggcgatg cctgggatcg tggccagctt ggcccgcagg
      361 gcatctgcct ggcctcgcag cgcgtcgagg tcctcgccga agatcttgat cgcgatctgc
      421 gagcgcacgc ccgagagcat gtggtcgatg cggtgcgaga tgggctggcc gatctccagc
      481 gcagccggca ggttgatcag gcgcttgcgg atgtcggcct tgatctcgtc catgctgcgc
      541 gtcagctcgg cggtgggctt gaggcccacg tcgagctcgc tgacgtgcac gccttccgcg
      601 tgttcgtcca gctcggcccg gccgctgcgg cgaccgacgt gcgtcacctc gggcacctgc
      661 ttgaccagta cctcggcctg gcgtgccaag gccgacgact ccgtcaaggt gacacccggg
      721 ttcaggcgca ggccgatcag cagcgtgccc tcgttgaacg gcggcaggaa ggtcgtcggg
      781 aagaacggca ccgccactgc ggccaccagc acggccgcac cggccgccgc catggcggcc
      841 ttgggccggt ccagcacgcc ctgcaggccg ctgcggtagc gtgccttcag ccaggccagg
      901 accttggtgt caccgtggtc caggttcttc atccgcggca gcaggtagaa gctgagcacc
      961 ggcgtcaccg tcaccgagac gatcagcgaa gccagcgtcg agacgatgaa ggcgatgccc
     1021 agcggcacga acagcttgcc ctccagcccc ggcagtgcaa acagcgggat gaagacgagc
     1081 acgatgatga ccgtcgcata gaggatggcc gatcgcactt ccatcgtcgc gtgcgcgacg
     1141 atctcgatcg ggtgcagccg gctgtgcgga tggttggccc ggtcggcctt cagccgccgc
     1201 agcacgttct cgacacccac caccgcatcg tcgacgagac cgccgatggc gatggccagg
     1261 ccgcccagcg tcatcgtgtt gatcgacagg ccgaagtact tgaagaccag cgcggtgatg
     1321 aagatcgata ccggaatggc cgtcagcgcg atgacggtcg gccgcaaggt gccgaggaag
     1381 aagaacagga tcaccgcgac gaagatcgag gcgccgatca gcttgccctg cagcgtcgtg
     1441 atcgaggcct cgatgaagct ggcctgacgg aaggtgacgc gaggcgcctc catgccggcc
     1501 ggcagcgaag ccttcagtcc gacgagggcg tcttcgatgg acttggtgag cgcgatggtg
     1561 tcggcagtcg gctgcttctg gatgcccagg atcaccgccg gcttgccctc aaatcctgcg
     1621 tcaccgcgct tgagtgccgc ggcgaacgtg acctcggcga tctggcgcag gaggatgggt
     1681 tggccgccct tggccgcgag cgcgaggttc ttcaggtcgt cgagccggga cgtgcggccg
     1741 aggttgcgga tcaggtactc gcggccgttc agctccagga agccacccga ggtgttcgac
     1801 gagtagccct tgagcgcgct ttcgagctgg tcgtgcgtga tgcccagctc ggccatgcgc
     1861 gcggtgttcg gctgcacctg gaactgccgc acctcgccgc cgatcgggat gacctgcgcg
     1921 acgcctggca ccgagagcag gcgcggtcga agcacccagt cggcgtactc gcgcaccgcc
     1981 atcgggctga tcttgctcgg atcgaccgga atggcgatct gcatgatctc gcccatgatc
     2041 gagctgatcg ggcccatgcg cggcaccacg ccttcggcga tgccttcctc catcgaggac
     2101 agccgctccg acaccagctg ccgggcgcgg aagatgtcgg tgctccagtc gaaggtgacg
     2161 tagaggaagg acaggccggc cgacgaggtc gagcgcacgc tttcgacgcc gggcagaccg
     2221 ttcatggtcg tctccagcgg gaaggtgatg agctgctcca cctcctcggc ggccatgccg
     2281 ccggcctcgg tcatgatggt gacggtcggc ttgttgaggt cggggaacac gtccaccggg
     2341 gtgcgcgaga gcgtgaacgc gccgtaggcc atcagaacga cgctggcgat gatcaccagc
     2401 agccggttcg tcaggctgtt gtcgagtagc cacttgaaca tgtcagcgca cctggttgat
     2461 cagggtggcg ccttgggtgg cgatgcggtc cccggccttc aggcccgatg tgaccgcgac
     2521 gttcacacca tcgagggcct cggtcgtgat gctgcgaggc tcgaagcgct cgggcgaagt
     2581 cttgacccag acgacggtct ggttggccgg gttcttcatc agcgacgcga ccgggaccgc
     2641 gatgcccttg accttctgct tcgtctgcac gaagacgcgc acgggttgtc ctacggccaa
     2701 gccgttcagg gccgcaccct gagcgcggaa gcccagcggc agcgcttgct cgcgcaggct
     2761 gcgcgccgca cccaggtact ccagcggcac acgctggtca ccgactgcca gcgtggcact
     2821 gccgacattg gccgcgagcg agggctcgaa ggccagtgcc tcgatgcgca agcggctcgg
     2881 gtcgatgatc tcgaacagca gttcgcgggc atcgaccacc tgaccggcca ccacatgggc
     2941 cgaggcgatc acgcccgaca ccggggccac cagcgcttcc cgcgttgcca ggccgccgcc
     3001 aacggccgcc atgcgctcgg ccaggctggc tgcctcgctt tcggccgcct cgatgtcctt
     3061 gcgcggcacg gtatccgaca actccttcag ccgcgtcacg cgcttgtcgg cgagcgactt
     3121 ggccgcgcgc aactcggcca attgcgcgga ttgattcgat ctctcgatgg gcgcggtcga
     3181 gggcaccacg taggccagca cctcgccctt gcgcaccacc tgtcccgggt tgggcaggcc
     3241 gcgtggaccg ggctcgatgc ggccggcgtt cagcggctgc accttgccgc cggcgttggg
     3301 atccatcagc accttgccgt tcagctcgac cgagcgcggc agctcggcct cagcagccac
     3361 catcgtacgt actcccagct ggcgctgggc cggcttgggc aggaacacgc tgccgtcggg
     3421 ctggcgttgg gggccgtttg cgctcggcgc agcgggtgcg tcgccgtggt catggccttc
     3481 cccggcctgc gcggtcagtg ggcccacgag tgccagcacc acgccgagcc aggccaggcg
     3541 atgcaaagaa gcgcgcttca tgctgcacct ccggcgcgaa cctggcgggc ggccatcagg
     3601 cgtctcccgc caaataccag cacggcaagg acggccaggc cgccggaggc ccatccgccg
     3661 acctccttcc atgaaagttc atgcgcaggc tcgtcggtgt gggcggcctc gtgcaagtcg
     3721 agttcgccgg ccagcagatc gacctccgcc ccagcggtca cggtcgcggt gatcggcagc
     3781 acgccgggct tgggcgcttc cttgagcacc acttcatacg cgtcgtcgcc atgggcctcg
     3841 gccttgaact tggtcccgtc gatctcgagt tcgatctgcg cgccacgcac cggggtgttg
     3901 tcgacgaatc ggtcgaggta gagcgtgacc tgcttgccat cgagcacgcc gacgagctcg
     3961 aaggtctcgg acaccgccgc aaagcggggc agcgcggtgc ccgtggcggc cggggcggtg
     4021 tcgccgtggt catggccatc gccggcgatg accggcaggg cgttgccgag cagcacggat
     4081 gcaaggcaga gcgcagccag ggagtgggaa gacttcatgg gatgggattc ctgatggggt
     4141 tcgctgagcg tgttcgtggg ggcgtgtgcc gtcggctcac tgcggcagca ggcccagggc
     4201 ctgccgccac gccgagatgg ctgcggcgag ttcgatgcgg gcgcgcacgg cctgtcgctc
     4261 ggcgtcggcg gcctcggcct cgatgcgcaa gcgcgtgggc aggtcggatt cgcccaggcg
     4321 gaaggacttg tcgaagaagc cgcgcgattc gcgcgccagt tgggcgcgac gatcggcagc
     4381 ggccagctgc gcccgcgccg cgtcggtgcg cgcgcgagcg gcttcgcgct cgcccgccag
     4441 gcgggcacgt tccaatgcca tctgcgcctg cagttcgacc gcctcggcgc gcgcagaggc
     4501 cacgcgggca tcgtgccggg gaccggcgcc gaaggggatg cgcacaccga ccgtgaaggt
     4561 ctgctggtag gactctccgt aagtgccccg atcgcgggtg gcggcgatgg tgagttccgg
     4621 gctggcgcga gactgcgtgg ccgcgagtgc agccacgccg tcggcgacgg cggagcggtc
     4681 cttcaacgag agcagttcgg catgcgtgtc caggccgtct gcaggaccgg tcgccgtgga
     4741 cggctcgggt tcggcgtcgg cgccgggcgc ggcgctgggt gcggcagccg ctggcatggc
     4801 gacgagggga cgaagctgct gccaggcggc cgtgagcgcg ccttcggcct gcgccacggc
     4861 cgcttcggcc gccgcgacgg cgccgtcggc ctggtgctgg tcggcacggg ccaggtcacc
     4921 ggctctgagc cgcttgccga catcggcggc aatgcgttga acgttgtcga gctggccgcg
     4981 tgcggcatcg agctccacac gggcccgctg ccacgcccac cacgcttcgc gcacagcggc
     5041 agccacgcgc agctgtgccg ccgtggtacg gctctcgatg gcacggccct cggcgtcggc
     5101 cagggcctgg ctgcggccac gttcgcccgg cagccacaat gggatggcca cgccgacctc
     5161 gacctcacgg gtgccgaggt ttcggttcag gcggtccgtc ttggtggaca gctccatcgc
     5221 cactggctcg ggcgtccatg cttgggccgc gtcccgctgc gcgcgtgccg cgtcgcgccg
     5281 cgctgccagc gcttgcgctt cgggctgccg cgcccaggcg gtttcgaagg ctgctcgcag
     5341 cgtgggcggg gtcggctcgc tgggtgcagc aggggtctgc gcacgcgctt caatcgcgac
     5401 gcccatcagc gctgcggtga gtgccgtact ggctacggtc gccagggtgg cgcgggcggc
     5461 gtgatgccag cgcccggccg gcatgattct tcttcggtgc atccgatgct ccagtgttga
     5521 aaacaaccca caggagatcg gcgagagtcg acgctgagga actcagggtc ggatcgggcc
     5581 tcgagcggcg ccgctgaccc atgtgtcggg cggcgcgcac gcaggcgcga acgggcttca
     5641 gaaggagaag gaacccgcct cgccgatcag gcgaggggca gccactgagg gcgttcgggc
     5701 gggctttgcg cgagggttcg cacggtgcct tcgacaggcg ttaccgggtg ggaggccaga
     5761 gccagcggcg aaatgtcgcc catgggcgcg ggcatgctgg cgcaggtgcc gtggcaatgg
     5821 ccgcagtcga aatcgaaccc agccggcgcg ctctgatctg ccggcgcgct gccctggtcg
     5881 gccgtggcct catccgtgtg ctggtgctcg tgatggccca ggtgctgaac atgctggccg
     5941 gtttcgtgcc cgcaatacgc cgccactgcc gcccagctga actggagcgg cagcagggcg
     6001 agcatgagga tcgcgagcca acggcgcatg aaaagagtat agcagctaca gagtttgaga
     6061 cgggttcgcc gggctgatcg ccgtgaccgg catcaccact tttcggcgcg gatgcgtcgg
     6121 acctgggcgt tcatgcggtg ccggatgacc tgcgtgaaga gccaacgctt gaggccggag
     6181 aggttcgagc cgtggcgcgc atagaactcg tccgggctgt cgaacacgcc gaagtcgtca
     6241 gcgatcccgt ccttctggat gtacgtgtcc tggccgcgcc attcaacgcc aggcgccgac
     6301 aggtcgggcg tggccgcgcc gtagccgcag aaccgctgtc ggaccgggaa gcgccgctgc
     6361 aggctggtca ggtagggtgc atcgaggatg aagccttcca ggttcaccca ccgtccctcg
     6421 aaggccactt cgacccaact gtggatgatg cgtcgcggtg ccagcacata ggcgagcccc
     6481 gtgatcgcac ccttctgcag cggcttgtcg atggtgaaac cgtggaagcg gcaggcaatg
     6541 cccacggcgc gcagcaaggc catcagcagc gtgcccttgg tgttgcactg gccgatgcca
     6601 tcggccagca ccgccgatgc cggcaactcg tcgccagcgt tgtagccgaa ggcgatctcg
     6661 ttgcgcacga agtcgtagac ggcgccgata cggtcataca tcggcaacgg ccgccacccc
     6721 cgcttctcga ccagcgcttc gatggcgggg tgcgtgaaat cgagcagccg cgtgggctgt
     6781 agcagttggg ctttggcctc catggtcgcc tcagcttcgt tcggggtttc gcaacaggcg
     6841 caggccgttg aagaccacca acaggctggc ccccatgtcg gcgaacacgg ccatccacat
     6901 gctggcgttg tcgaac
//
LOCUS       DAOMVO010000114         6907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2940_length_6907_cov_14.130765, whole genome shotgun sequence.
ACCESSION   DAOMVO010000114 DAOMVO010000000
VERSION     DAOMVO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2940_length_6907_cov_14.130765"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="bamB"
                     /locus_tag="PKV30_07040"
     CDS             <1..1068
                     /gene="bamB"
                     /locus_tag="PKV30_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HRL30004.1"
                     /translation="GCAGGSARPKPAELPPNAALVGVRQAWTARLPAVGFPLQVNVLG
                     QQVTLAGTDGTIVTLDAASGRETGRASAGAPLSAGVGSDGRVSAVVTRDNRVVALEGG
                     RELWRHKLATQSYTAPLVAGGRVFVLGADRSLSAFDGRNGARLWHLDRPAEPLVLRQA
                     GMLQAVGNTLVAGMAGRLVGVDPDTGTVRWEAPLASARGTNDVERLIDLVGGVSRDGD
                     VLCARSFQTAVGCVDTASGNLKWTKPASGATGLAGDGQRLFGTESDGRVLAWRAETGD
                     RAWATDALQWRQLTGPLVLGRSVIVGDSTGLVHMLSREDGSPLNRLATDGSGIAATPV
                     VAGNTLVVVTRSGGVYGFVPE"
     gene            1299..2639
                     /gene="der"
                     /locus_tag="PKV30_07045"
     CDS             1299..2639
                     /gene="der"
                     /locus_tag="PKV30_07045"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRL30005.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTKSRDAIVADFAGLTRDRHYGQ
                     GHLGKREYIVIDTGGFEPTAESGIFKEMAKQTRQAVAEADVVIFVVDARAGLSAQDHD
                     IADYLRRLGKPCLLVANKAEGMREGAQLAEFYELGLGEVLPVSAAHGQGVRSMLEAAL
                     DALDLPEPEEDAEPDPDAPIRLAVAGRPNVGKSTLINTWLGEERLVAFDMPGTTRDAI
                     SVPFERAGKKFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIESANVVLLLLDATQGVT
                     EQDAHIAGFILDAGRAVVLAVNKWDAVDAYQRELVQRSIETRLAFLKFAEIHFISAKK
                     RQGLGPLWGSIIRAHKAAMRKMPTPQLTRLLLEAVQFQQPKRSGMYRPKLRYAHQGGM
                     NPPVIVVHGNSLEGVTDAYKRFLEGRFRKAFDLTGTPLRVEMKTARNPYADKES"
     gene            2784..3035
                     /gene="hfq"
                     /locus_tag="PKV30_07050"
     CDS             2784..3035
                     /gene="hfq"
                     /locus_tag="PKV30_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRL30006.1"
                     /translation="MSNKGQLLQDPFLNTLRREHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTIVPGRAVNFSSAGDTPDSEEK"
     gene            3118..4335
                     /gene="hflX"
                     /locus_tag="PKV30_07055"
     CDS             3118..4335
                     /gene="hflX"
                     /locus_tag="PKV30_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRL30007.1"
                     /translation="MPATANPERPAAVLVGVDLGAPHFDRELEELGLLAQTAGMQPVA
                     RITCKRQAPDAALFVGSGKADEIRLLAETQGAQEVLFDQALSPVQQRNLERAIGLPVN
                     DRTLLILEIFAQRARSHEGKLQVELARLQYLSTRLVRRWSHLERQQGGIGGRGGPGET
                     QIELDRRMIGESIKRTKERLAKVKRQRQTQRRQRERQGAFNISLVGYTNAGKSTLFNA
                     LVKARAYAADQLFATLDTTTRQLYLGDGQGAGRSVSLSDTVGFIRDLPHGLVDAFEAT
                     LQEAADADLLLHVVDAANPNHLEQIDEVQRVLREIGAAEVPQLLVFNKLDGLDAARRP
                     HALKDTLAQDGRELTRVFVSARTGEGLAELRAELAARAQSMVDTAAPAAEDARGLLPI
                     GHNHGLHPQEFNE"
     gene            4332..>4896
                     /locus_tag="PKV30_07060"
     CDS             4332..>4896
                     /locus_tag="PKV30_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HRL30008.1"
                     /translation="MTARTSAPRLAHWSGRLRGMFNLNDPQWGRGDGASGQDNPRPDD
                     QRPADPPHAPPPPPPPPRGNGPNQGPPDLDELWRDFNRKLTGLFGGGRGRGPGNGGLG
                     GGGGFQPDMKGAGIGVGMIAAVAVVIWLASGIFIVREGEQAVVTTFGKYDSTRNAGLN
                     WRMPFPIQRHETVRVTQIRSVDVGRDSV"
     assembly_gap    4897..4996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4997..5860
                     /gene="hflK"
                     /locus_tag="PKV30_07065"
     CDS             <4997..5860
                     /gene="hflK"
                     /locus_tag="PKV30_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRL30009.1"
                     /translation="ETVRVTQIRSVDVGRDSVIKSTGLRESAMLTQDENIVEIKFAVQ
                     YRLNDARAYLFESKSPDDAVVQAAESAVREVVGKMSMGDAMADERDQIAPRVRDLMQT
                     ILDRYRVGIEVVAVNMQQGGVRPPEQVQAAFDDVLKAGQERERVKNEAQAYANDVVPR
                     AAGTAARLKQEADGYKARIVAQAQGDTQRFSSVLTEYQKAPQVTRERMYLDAMQEIYS
                     GVNKVIIDSRAAGNLLYLPIDKLMQSAGAPAAAVPGPAAQAPAPAPAAQAPAADARTR
                     DNARSRERETR"
     gene            5881..6768
                     /locus_tag="PKV30_07070"
     CDS             5881..6768
                     /locus_tag="PKV30_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRL30010.1"
                     /translation="MNKVGFIGTTVLIVLALLASTMFVVDQRQFGVVYQLGQIQKVIT
                     EPGLNFKLPPPFQNVSYIDKRLLTLDSMDTEPMLTAEKQRVVIDWYVRWRITDPQAYI
                     RNVGLDENAGAMQLNRVVRNAVQEEINRRTVRELISTRRETLMADVKREVTEAVKGDK
                     PWGIDIVDVRITRADYVESITESVYRRMQAERQRVANELRSTGAAEGEQIRADADRQR
                     EVTVANAARDAEKVRGEGDAEATRIYGEAFGKDPQFAEFYRSLDAYKASIGKKGDVLV
                     IDPASSDFFKNMRVAPTRR"
     gene            6774..>6907
                     /locus_tag="PKV30_07075"
     CDS             6774..>6907
                     /locus_tag="PKV30_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRL30011.1"
                     /translation="MDGSTWLAALGLALILEGLLPFIAPSAWRQAFTQLMQMADGQLR
                     "
BASE COUNT         1185 a         2333 c         2299 g          990 t
ORIGIN      
        1 ggttgtgccg gcggttccgc gcggcccaag ccggccgagc tgccgcccaa tgcggcgctg
       61 gtcggcgtgc gccaggcctg gacggcgcgc ctgcccgccg tgggctttcc gctgcaggtc
      121 aacgtgctcg gccagcaggt cacgctggcg ggcaccgacg gcaccatcgt cacgctggat
      181 gccgccagcg gccgcgaaac cgggcgcgcc agcgccggcg cgccgctgtc ggccggtgtc
      241 ggcagcgatg gccgcgtgag cgccgtggtg acgcgcgaca accgcgtggt ggcgctggaa
      301 ggcgggcgcg agctatggcg ccacaagctg gccacgcaga gctacaccgc gccgctggtg
      361 gccggcggcc gcgtgttcgt gctgggcgcc gaccgttcgc tgagcgcctt cgatggccgc
      421 aacggtgcgc gcctgtggca cctggaccgc ccggccgagc cgctggtgct gcgccaggcc
      481 ggcatgctgc aggccgtggg caacacgctg gtggcgggca tggcggggcg gctggtgggc
      541 gtcgaccccg acaccggcac agtgcgctgg gaggcgccgc tggcctcggc ccgcggcacc
      601 aacgacgtgg agcgtctgat cgatctggtg ggcggcgtga gccgcgacgg cgacgtgctg
      661 tgtgcgcgct cgttccagac cgccgtgggc tgcgtcgaca ccgccagcgg caacctgaag
      721 tggaccaagc ccgccagtgg tgccactggc ctggccggtg acggccagcg cctgttcggc
      781 accgaatccg acggccgcgt gctggcctgg cgcgccgaaa ccggcgaccg cgcctgggcc
      841 accgatgccc tgcaatggcg ccagctcacc ggcccgctgg tgctgggccg ctccgtcatc
      901 gtgggcgaca gcaccggcct ggtgcacatg ctgtcgcgcg aggacggcag cccgctgaac
      961 cgcctggcca ccgatggctc gggcatcgcc gccacgccgg tggtggcggg caacacgctt
     1021 gtggtggtca cgcgcagcgg tggcgtgtac ggcttcgtgc ctgagtgact ttgattcaat
     1081 aaaaatagct gcttgcgctt ggtatacaag gatttcaggt atgaaagtgc ctgaaatcgg
     1141 cttgtaacaa gcgcaagcag ctatcatttt gttgaggcag atccggcgta cctgtgatgg
     1201 ccgccgagcc cgtactattg cagcttcaac gccattggcg caggccggtc tgacccgacc
     1261 gccgcgcggc gcctgcctcc ccctcgcgaa ccgtcgccat gaaacccgtt atcgccctgg
     1321 tgggccgccc caacgtgggc aaatccacgc tgttcaaccg gctgaccaag agccgcgatg
     1381 ccatcgtggc cgactttgcc gggctgacgc gcgaccgcca ctacggccag ggccacctgg
     1441 gcaagcgcga atacatcgtc atcgacaccg gcggcttcga gcccacggcc gagtccggca
     1501 tcttcaagga gatggccaag cagacccgcc aggccgtggc cgaggcggac gtggtcatct
     1561 tcgtggtcga cgcgcgtgcc ggcctgtcgg cgcaggacca cgacatcgcc gattacctgc
     1621 ggcgcctggg caagccctgt ctgctggtgg ccaacaaggc cgagggcatg cgcgagggcg
     1681 cgcagctggc cgagttctac gagctgggcc tgggcgaggt gctgccggtg tcggctgcgc
     1741 acggccaggg cgtgcgcagc atgctcgagg ccgcgctgga cgcactggac ctgcccgagc
     1801 ccgaggaaga cgccgagccc gatcctgacg cgcccatccg cctggccgtg gcggggcggc
     1861 ccaacgtggg caagtccacc ctcatcaaca cctggctggg cgaggagcgc ctggtcgcgt
     1921 tcgacatgcc cggcaccacg cgcgacgcca tcagcgtgcc gttcgagcgc gccggcaaga
     1981 aattcgagtt gatcgacacc gccggtctgc gccgcaaggg caaggtgttc gaggcgatcg
     2041 agaagttctc cgtcgtcaag acgctgcagg ccatcgaatc ggccaacgtc gtgctgctgc
     2101 tgctggacgc cacgcagggc gtgaccgagc aggacgcgca catcgccggc ttcatcctcg
     2161 acgccggccg cgccgtggtg ctggcggtga acaaatggga cgcggtggat gcttatcagc
     2221 gcgagctggt gcagcggtcg atcgagacgc ggctggcgtt cctgaaattt gccgagatcc
     2281 atttcatctc ggccaagaag cggcaaggcc tgggcccgct gtggggctcg atcatccgcg
     2341 cgcacaaggc cgccatgcgc aagatgccga cgccgcagct cacgcgcctg ctgctggaag
     2401 cggtgcagtt ccagcagccc aagcgcagcg gcatgtaccg acccaagctg cgctatgcgc
     2461 atcagggcgg catgaacccg ccggtgatcg tggtgcatgg caattcgctc gaaggcgtga
     2521 ccgacgccta caagcgcttc ctggaagggc gttttcgcaa agcttttgac ctgacaggca
     2581 cgccgctgcg ggttgaaatg aaaacagcgc ggaaccctta cgccgacaaa gagtcctagg
     2641 gtggcgccgg ggccttgtgt gtaggccgag atcgccaatg cgccccaagc cgggcgtttt
     2701 ctgtctcttg acatgcgttt agccaacccc atgtgttaac ttccatcctc tttcaacgat
     2761 tcaccgaaca cggagaaaat atcgtgagca acaaagggca actcctacaa gacccgtttc
     2821 tgaacaccct gcgtcgtgag cacatcccgg tgtccattta cctggtcaac ggcatcaagc
     2881 ttcagggtca gatcgaatcc tttgaccagt acgtggtgct gctgcgcaac acagtcacgc
     2941 aaatggtcta caagcacgcc atttcaacca tcgtgccggg ccgcgccgtc aacttctcca
     3001 gcgccggcga cacgccggat tctgaagaaa aataagcgcc agcgcgcgtc cagcctgcgc
     3061 gagcagctct atttaatata gctaccgcgc gcggcacatc agactgtcat caccccattg
     3121 cccgccaccg ccaaccctga acgtcccgct gccgtcttgg tcggcgtgga cttgggcgcc
     3181 ccccatttcg accgcgagct ggaagagctg ggcctgctgg cgcaaacggc cggcatgcag
     3241 ccggtcgcgc gcatcacctg caagcgccag gcgccggacg cggcgctgtt cgtcggcagc
     3301 ggcaaggcgg atgaaatccg cctgctggcc gagacccaag gcgcgcaaga ggtgctgttc
     3361 gaccaggccc tcagcccggt gcagcagcgc aatctggagc gcgccatcgg cctgccggtc
     3421 aacgaccgca cgctgctgat tcttgaaatc ttcgcccagc gcgcccgcag ccacgagggc
     3481 aagctgcagg tcgaactggc gcgcctgcaa tacctcagca cgcgcctggt gcgccgctgg
     3541 agccacctgg agcgccagca gggcggcatc ggcggccgcg gcggccccgg cgagacgcag
     3601 atcgaattgg accgccgcat gatcggcgaa tcgatcaagc gcaccaagga gcgcctcgcc
     3661 aaggtcaagc gccagcgcca gacgcagcgc cgccagcgcg agcgccaggg cgcgttcaac
     3721 atttcgctgg tcggctacac caacgccggc aagtccacgc tgttcaacgc gctggtcaag
     3781 gcgcgcgcct atgcggccga ccagttgttt gccacgctcg acaccaccac gcgccagctg
     3841 tacctgggcg atgggcaggg ggccgggcgc tcggtgtcgc tgtcggacac cgtgggcttc
     3901 atccgcgacc tgccgcacgg cctggtcgac gcctttgagg ccacgctgca agaggcggcc
     3961 gatgccgacc tgctgctgca cgtggtcgat gccgccaacc ccaatcacct ggaacagatc
     4021 gacgaagtgc agcgggtgct gcgcgagatc ggtgctgccg aggtgccgca actgctggtc
     4081 ttcaacaagc tcgacgggct ggacgccgcg cgccggccgc acgcgctgaa agacacgctg
     4141 gcgcaggatg gccgcgagct gacgcgggtg tttgtcagcg cgcgcacggg cgaagggctg
     4201 gccgagctgc gcgccgagct ggccgcgcgc gcgcaatcga tggtcgacac cgctgccccg
     4261 gcggccgaag acgcccgtgg gctgttgccg attgggcaca atcacggctt gcaccctcaa
     4321 gaattcaacg aatgaccgca cgcacgagcg ccccgcggct ggcccactgg tcaggccgcc
     4381 tccgaggcat gttcaacctg aacgacccgc aatgggggcg tggcgatggc gcgtccggcc
     4441 aggacaaccc gcgccccgac gaccagcgcc cggccgatcc gccacatgct ccgccaccac
     4501 ccccgccgcc gccgcggggc aacggcccca accagggccc gcccgatctg gacgagctgt
     4561 ggcgcgactt caaccgcaag ctcaccggcc tgttcggcgg tggccgcggg cgcggccccg
     4621 gcaatggcgg cctgggtggt ggcggtggtt ttcagcccga catgaagggc gccggcatcg
     4681 gcgtcggcat gatcgcggcg gtggccgtgg tgatctggct ggccagcggc atcttcatcg
     4741 tgcgcgaagg cgagcaggcc gtggttacca ccttcggcaa gtacgactcg acgcgcaacg
     4801 ccggcctgaa ctggcgcatg ccgttcccca tccagcggca cgagacggtg cgcgtgacgc
     4861 agatccgctc ggtcgacgtc ggccgcgaca gcgtcannnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnngaga cggtgcgcgt gacgcagatc cgctcggtcg acgtcggccg
     5041 cgacagcgtc atcaagagca ccggcctgcg cgaatcggcc atgctgacgc aggacgagaa
     5101 catcgtcgag atcaagttcg ccgtgcagta ccgtctgaac gacgcccgtg cctacctgtt
     5161 tgagagcaag agccccgacg atgccgtggt gcaggccgcc gaaagcgccg tgcgcgaggt
     5221 ggtcggcaag atgagcatgg gcgacgccat ggccgacgag cgcgaccaga tcgcgccgcg
     5281 cgtgcgcgac ctgatgcaga ccatcctgga tcgatatcgt gtaggcatcg aggtggtggc
     5341 cgtgaacatg cagcagggcg gcgtgcgccc gcccgagcag gtgcaggccg cgttcgacga
     5401 cgtgctgaaa gccggccagg agcgcgagcg cgtcaagaac gaggcgcagg cctacgccaa
     5461 cgacgtggtg ccgcgcgccg ccggtaccgc cgcgcgtctc aagcaagagg ccgacggcta
     5521 caaggcccgc atcgtggcgc aggcgcaggg cgatacgcag cgcttctcgt cggtgctgac
     5581 cgaataccag aaggcgccgc aggtcacgcg cgagcgcatg tacctggacg ccatgcagga
     5641 aatctacagc ggtgtgaaca aggtcatcat cgacagccgc gcggccggca acctgctgta
     5701 tttgccgatc gacaagctca tgcaatcggc cggcgcgccg gcggctgcgg tgcccgggcc
     5761 ggccgcccaa gcgcctgccc cggcgccggc cgcacaggcg ccagcggccg acgcccgcac
     5821 gcgcgacaac gcccgcagcc gcgagcgcga gacgcgctga tcgagggagc acacgacaaa
     5881 atgaacaaag tcggattcat cggcaccacc gtcctcatcg tgctggcctt gctggcctcg
     5941 accatgttcg tggtcgatca gcgccagttt ggcgtcgtct accagctggg ccagatccag
     6001 aaggtgatca ccgagccggg gctcaacttc aagctgccgc cgccgttcca gaacgtcagc
     6061 tacatcgaca agcgcctgct cacgctcgac agcatggaca ccgagcccat gctgacggcc
     6121 gagaagcagc gcgtcgtcat cgactggtac gtgcgctggc gcatcaccga tccacaggcc
     6181 tacatccgca acgtgggcct ggacgagaac gccggcgcca tgcaactcaa ccgcgtggtg
     6241 cgcaacgccg ttcaggaaga aatcaaccgc cgcaccgtgc gcgaattgat ctcgacccgc
     6301 cgcgagacgc tgatggccga tgtcaagcgc gaggtgaccg aagccgtcaa gggtgacaag
     6361 ccctggggca tcgacatcgt cgacgtgcgc atcacgcgcg ccgactacgt ggaatcgatc
     6421 accgaatcgg tgtaccgccg catgcaggcc gagcgccagc gtgtggccaa cgagttgcgc
     6481 tccaccggcg ccgccgaagg cgagcagatc cgcgccgatg ccgaccgcca gcgcgaggtg
     6541 acggtggcca acgccgcgcg cgacgccgag aaagtgcgcg gcgaaggcga tgccgaagcc
     6601 acgcgcatct atggcgaggc ctttggcaag gacccgcagt tcgccgagtt ctaccgcagc
     6661 ctggacgcct acaaggccag catcggcaag aagggcgacg tgctggtgat cgacccggcg
     6721 tcgtcggact tcttcaagaa catgcgcgtc gcgccgaccc gccgctgaac accgtggacg
     6781 gcagcacttg gctggccgcg ctgggcctgg cgctgatcct tgagggcctg ctgcccttca
     6841 tcgcgcccag cgcttggcgc caggccttca ctcagctgat gcagatggcg gacgggcagc
     6901 tgcgctt
//
LOCUS       DAOMVO010000115         6834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_2996_length_6834_cov_15.549491, whole genome shotgun sequence.
ACCESSION   DAOMVO010000115 DAOMVO010000000
VERSION     DAOMVO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_6834_cov_15.549491"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /locus_tag="PKV30_07080"
     CDS             <1..2298
                     /locus_tag="PKV30_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HRL30012.1"
                     /translation="GLLSTRARLNASFAHIPARPGQLAFVSQSGALVTAMLDWAEARQ
                     IGFSHFVSLGEHADVDFGDMLDYLGGDADTRAILLYIESIDQARKFMSAARSAARNKP
                     VIVIKAGRSAQGQQAAASHTGALAGADNVVDAAIARAGMLRVDTLEQLFLAAEILTRY
                     QAPIGDRMTVLTNGGGVGVLAADAAATEDVPLADLSAPLKRALDQVLPANWSHGNPVD
                     IIGDAPAERYVDALKALGQHPADTGTLLFIHAPTAIVPPTDIARAMIPVIRPAGQQPL
                     PLVSAWVGGPSVAEARRLFVDAGIACYDLPEQAVMAIGMLQRYARNRAELSEAPPAAQ
                     LHNGSTPDVARVRALMQSVLASGRDMLTEPEAKAVCEAYHIPVVATRTVPADADAAAA
                     EAARIGFPVVLKILSEDISHKSDVGGVVLNLNDEGDVRAAAQAMLVRVDKSHPDARVD
                     GFTVQKMVKMKQAQELIIGASIDPTFGPVILFGQGGTAVEVVADSAMALPPLNAPLAR
                     ALIERTRVARLLHGYRDVPPADIDAVIATLMAVSQLLADVPEVAELDINPLIVNHEGA
                     IALDARVRVSRQQPAGAAHFAIQPYPGELTEVIDWKGQPLTLRPIRPEDEARHRAFID
                     DLDAEDLRMRYFYSRRVMERSELARLVQVDYTREMAFIATVPGADGEEKTLGVARAIA
                     DPDNLGAEFGIILRPELKGSGLGRILLDKLIRYQRAAGTERLVATVLTENRRMRELAR
                     ALGFSEHAQPGDAGTLALALDLQRG"
     gene            2371..3609
                     /locus_tag="PKV30_07085"
     CDS             2371..3609
                     /locus_tag="PKV30_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRL30013.1"
                     /translation="MNRTQSLLRLSAWALGAATLLAAPALHAQDAPPAVDPAQFSACL
                     NELKAAPAFSRITPATFVRATDGLQADPSVLVLLNRQPEFSMPVWDYIAVLVDDERVA
                     DGRAAHARWRDALQKIEQQSGVAPNVVIGVWGVESNFGQNLGGRPLVQSLATLSCFGR
                     RQSYFRGEFAAALRILQEGHIAPEKLVGSWAGAFGQTQFMPSTFFRSAVDFDGDGRRD
                     IVDSVPDALASTAKFLQNAGYRRGEPWGFEVRLPPGLDTSDASRKNKRPIDAWRSAGV
                     TLADGSPLPGSLPSAGLMIPARGGPAFLVGRNFDTLYSYNASENYALAIAQLSNLVAN
                     PGGSVAFVTPWPTDDAGLSRAQNRELQTLLLARGHDIGSADGMIGAKTREAIKAEQQK
                     LGMKPDGRAGQKLLAALRRG"
     gene            3638..4288
                     /locus_tag="PKV30_07090"
     CDS             3638..4288
                     /locus_tag="PKV30_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30014.1"
                     /translation="MTPADHSPDTAARPRWRALAGPLLAAGYAVWCLALLWLQPRSLL
                     AIASFLLPCYLFICSWLWRRGWRPVAVGVALGLPALLAWAYPRYAARAEIFYVAEYAL
                     VYATLCAWFASSLAGTPVITRVARRVHPLTPDMQAYTVRLTRGWALYFALMAALSLLV
                     FALLGLPAWAFFTVVLSPISLASFLIGEHLLRYRWHPEFDRASLRHTIRTWREGGA"
     gene            complement(4302..5330)
                     /locus_tag="PKV30_07095"
     CDS             complement(4302..5330)
                     /locus_tag="PKV30_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRL30015.1"
                     /translation="MKKSFAVPACLAGLSLLAPLAHAQSSVQLSGLADMYVGSMRMAG
                     DAERKNTVGNNGMTTSWFGVTGTEDLGNGLKAGFAFTSFLRMSNGSYGRFVNDTFWSR
                     DANVSLSGNFGTVRLGRWMAPNFLPTILFNPFGDSFTFSPLVLHANVPLFNGTGWTGT
                     TPADTGWSSQIAYTTPSFGGLRGTLQYQFRDGAASGKNWGGNLIYMNGPLGLTAFYER
                     AQMSNPVAANFADGSVRTDWMLGGFYDFQVVKLFLTYGQAKNDVSNAKLKTAQLGASV
                     PIGAGKLLASYAQTRNNLLDAKRQTLTVGYDYNLSKRTDLYANVMHDRITNRSSGTSY
                     GIGLRHRF"
     gene            complement(5672..>6834)
                     /gene="glmS"
                     /locus_tag="PKV30_07100"
     CDS             complement(5672..>6834)
                     /gene="glmS"
                     /locus_tag="PKV30_07100"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRL30016.1"
                     /translation="VQRPVKTVQAMSGAAELGPYRHYMQKEIFEQPRAIADTLEGVEA
                     IVPELFEAANNHAPGEGAYPTFQSIRQVLILACGTSYYSGCVAKYWIESIAKLPCQVE
                     IASEYRYRESVPDPATLVVTITQSGETADTLSALRHAQSLGMRHTLTVCNVATSAMVR
                     ECKLAYITRAGIEIGVASTKAFTTQLAGLFLLTLALAKSRGLLTQEQEHDHLKAMRHL
                     PSALQAVLALEPQIITWAEQFAGKENALFLGRGLHYPVAQEGALKLKEITYIHAEAYA
                     AGELKHGPLALVTSSMPVVTVAPNDALLEKLKSNMQEVRARGGVLYALADDDTHIQSA
                     DGLHVIRMPGHYGPLSPILHVVPLQLLAYHTAVARGTDVDKPRNLAKSVTVE"
BASE COUNT         1047 a         2298 c         2345 g         1144 t
ORIGIN      
        1 gggctgctca gcacccgcgc gcggctgaac gcgagctttg cgcacattcc ggcgcggccg
       61 ggccagctgg cgtttgtctc gcaatcgggc gcgctggtca ccgccatgct ggactgggcc
      121 gaggcgcggc agatcggctt ttcgcacttc gtctcgctgg gcgagcacgc cgatgtcgac
      181 tttggcgaca tgctcgacta cttgggcggc gacgccgaca cgcgcgccat cctgctgtac
      241 atcgaatcga tcgaccaggc gcgcaagttc atgtcggccg cgcgctcggc ggcgcgcaac
      301 aagccggtca tcgtcatcaa ggccggccgc agcgcgcagg gccagcaggc cgccgcctcg
      361 cacaccggcg cgctggccgg cgccgacaac gtggtcgatg ccgccatcgc ccgcgccggc
      421 atgctgcgcg tggacacgct ggagcagttg tttctggcgg ccgagatcct gacccgctac
      481 caggcgccca tcggcgaccg catgacggtg ctgaccaacg gcggcggcgt cggcgtgctg
      541 gcggccgacg cggcggccac cgaagacgtg ccgctggccg atttaagcgc gcccctgaag
      601 cgcgcgcttg atcaggtgct gcccgccaac tggtcgcacg gcaacccggt ggacatcatt
      661 ggcgacgcgc cggccgagcg ctacgtcgac gcgctgaagg cgctcggcca gcacccggcc
      721 gataccggca cgctgctgtt catccacgcg cccaccgcca tcgtgccgcc gaccgacatt
      781 gcgcgcgcca tgattccggt catccgacct gctgggcagc agccactgcc gctggtcagc
      841 gcctgggtcg gcggcccgtc ggtggccgag gcgcggcgtc tcttcgtgga tgccggcatc
      901 gcctgctacg acctgccgga gcaggccgtg atggccatcg gcatgctgca gcgctacgcg
      961 cgcaaccgcg ccgagctgag cgaggcgccg cccgccgcgc aattgcacaa cggcagcacc
     1021 cccgacgtgg cgcgcgtgcg cgccctgatg caaagcgtgc tggccagcgg ccgcgacatg
     1081 ctgaccgagc ccgaagccaa ggcggtgtgc gaggcgtacc acatccccgt ggtggccacg
     1141 cgcacggtgc cggccgatgc cgacgcggca gcggccgagg cggcgcgcat cggctttccg
     1201 gtggtgctca agattctttc ggaagacatt tcgcacaagt ccgacgtcgg cggcgtggtg
     1261 ctgaacctga acgacgaagg cgacgtgcgc gccgccgcgc aggccatgct ggtgcgcgtg
     1321 gacaagagcc accccgacgc gcgcgtggac ggtttcaccg tgcagaagat ggtgaagatg
     1381 aagcaggcgc aagagctgat catcggcgcc agcatcgacc cgacctttgg ccccgtgatc
     1441 cttttcggac agggcggcac ggcggtcgag gtggtggccg acagcgccat ggcgctgccg
     1501 ccgctcaacg ccccgctggc gcgcgcgctg atcgagcgca cgcgcgtcgc caggctgctg
     1561 cacggctacc gcgacgtgcc gccggccgac atcgatgccg tcatcgccac gctgatggcc
     1621 gtgtcgcaac tgctggccga cgtgcccgag gtggccgagc tggacatcaa cccactcatc
     1681 gtcaaccacg aaggcgccat cgcgctcgac gcccgcgtgc gcgtgagccg ccagcagccg
     1741 gccggcgcgg cgcacttcgc catccagccc tacccgggcg agttgacgga ggtgatcgac
     1801 tggaagggcc agccgctcac gctgcgcccg atccggccgg aagacgaggc gcgccatcga
     1861 gccttcatcg acgacctgga cgccgaagac ctgcgcatgc gctacttcta cagccgccgc
     1921 gtgatggagc gcagcgaact ggcgcgcctg gtgcaggttg actacacgcg cgagatggcc
     1981 ttcatcgcca ccgtgcccgg cgccgatggt gaggagaaaa cgctgggcgt ggcgcgcgcc
     2041 attgccgacc cggacaatct gggcgctgag ttcggcatca tcctgcggcc tgagctgaaa
     2101 ggcagcggcc tcggccgcat cctgctggac aagctgatcc gctaccagcg cgccgccggc
     2161 accgagcggc tggtggcgac cgtgctgacc gagaaccgcc gcatgcgcga gctggcgcgt
     2221 gcgctcggct tcagcgagca cgcgcagcca ggcgacgccg gcacgctggc gttggcgctg
     2281 gatctgcaac gcgggtgacg aaacgtgtgt tcatgtatcc attcaccctc tggcgcacga
     2341 ctgatgtgcg caatcagcta tcatttttga atgaacagaa cccaaagcct tcttcgcctg
     2401 tctgcttggg cgctgggcgc cgccacgctg ctggccgcgc cggcgctgca cgcgcaggat
     2461 gcgccgcctg ccgttgaccc ggcgcagttc agcgcatgct tgaacgagtt gaaagccgcg
     2521 cccgccttca gccgcatcac gcccgccacc tttgtgcgag cgaccgacgg cctgcaggcc
     2581 gatccgtcgg tgctggtgct gctcaaccgc cagcccgagt tcagcatgcc ggtgtgggat
     2641 tacatcgccg tgctggtcga cgacgagcgc gtggccgatg gccgcgcagc gcacgccagg
     2701 tggcgagatg cgcttcaaaa aatagagcaa caaagcggcg ttgcgcccaa cgtggtgatc
     2761 ggcgtgtggg gtgtggagag caacttcggc cagaacctgg gcggccgccc gctggtgcag
     2821 tcgctggcca cgctgtcgtg ttttggtcgc cggcaaagct actttcgcgg cgagttcgcg
     2881 gcggcgctgc gcatcttgca agagggccac atcgcgcccg aaaaactggt cggctcgtgg
     2941 gccggcgcct tcgggcagac gcagttcatg cccagcacct tttttcgcag cgcggtcgat
     3001 ttcgatggcg acgggcggcg cgacatcgtt gattcggtgc cggatgcgct ggcatccacg
     3061 gccaagtttt tgcagaacgc gggctacagg cgcggcgagc cctggggctt tgaagtccgt
     3121 ctgccgcccg gcctggacac ttcggacgcc agccgcaaga acaagcgccc gatcgacgcc
     3181 tggcgcagcg ccggcgtgac gctggccgac ggctcgccgc tgcccggcag cctgcccagc
     3241 gcggggctga tgattccggc gcggggcggg cccgccttcc tggtcggccg caacttcgac
     3301 acgctgtaca gctacaacgc gagtgaaaac tacgcgctgg cgattgcgca gctgtcgaac
     3361 ctggtggcca accctggcgg cagcgtggcc ttcgtcacgc cctggccgac cgacgacgcc
     3421 ggcctttcgc gcgcgcagaa ccgcgagctg caaaccctgc tcttggcgcg cggccacgac
     3481 atcggcagtg ccgacggcat gatcggcgcc aagacgcgcg aagccatcaa ggccgagcag
     3541 caaaagctgg gcatgaagcc cgacggccgc gccgggcaaa agctgctggc ggcgctgcgg
     3601 cgcggctgac gcgcgctcgc gtcgctatga ttttggggtg acgcccgccg accattcacc
     3661 cgacaccgcc gcgcgcccgc gctggcgcgc gctggccggc ccgctgctgg ccgcgggcta
     3721 cgcggtgtgg tgcctggcgc tgctgtggct gcagccgcgt tcgctgctgg ccatcgccag
     3781 ttttctgttg ccgtgctacc tgttcatctg ctcgtggctg tggcggcgcg gctggcggcc
     3841 ggtggcggtg ggggtggcgc tggggctgcc cgcgctgctg gcctgggctt atccgcgcta
     3901 tgcggcgcgc gcagaaattt tctacgtcgc cgaatacgcg ctggtctacg ccacgctgtg
     3961 cgcctggttc gccagttcgc tggccggcac gccggtcatc acgcgggtgg cgcggcgcgt
     4021 gcacccgctc acgcccgaca tgcaggccta caccgtgcgg ctgacgcgcg gctgggcgct
     4081 gtactttgcg ctgatggcgg cgctgtcgct gctggtcttt gcgctgctgg gcctgccagc
     4141 ctgggccttc ttcaccgtgg tgctgtcgcc gatttcgctg gcgagctttt tgatcggcga
     4201 gcacctgctg cgctaccgct ggcacccgga gttcgaccgc gcctcattgc gccacaccat
     4261 ccgcacctgg cgcgagggcg gcgcctgagg cgccgcgctg atcagaaccg gtggcgcagc
     4321 ccgatgccgt agctggtgcc gctgctgcgg ttggtgatgc ggtcgtgcat cacgttggcg
     4381 tacaggtcgg tgcgcttgga caagttgtag tcgtagccca cggtgagcgt ctggcgcttg
     4441 gcgtcgagca ggttgttgcg cgtctgcgcg tacgaagcca gcagcttgcc ggcgccaatg
     4501 ggcaccgagg cgcccagttg cgcggtcttc agcttggcgt tgctgacgtc gttcttggcc
     4561 tggccgtagg tcaggaacag cttgacgacc tggaagtcgt agaagccgcc cagcatccag
     4621 tcggtccgca cggagccatc ggcaaagttg gcggccaccg gattgctcat ctgcgcgcgc
     4681 tcgtagaacg cggtcaggcc gagcgggccg ttcatgtaga tcaggttgcc gccccagttc
     4741 ttgccgctgg ccgcaccatc gcggaactgg tattgcagcg tgccgcgcag gccgccaaaa
     4801 ctgggcgtgg tgtaggcgat ctggctcgac cagccggtgt cggccggcgt ggtgccggtc
     4861 cagccggtgc cgttgaacag cggcacgttg gcgtgcagca ccagcggcga gaaagtgaac
     4921 gaatcgccga acgggttgaa caggatggtg ggcaggaagt tgggcgccat ccagcggccc
     4981 aggcgcacgg tgccgaaatt gcccgacagg ctgacgttgg cgtcgcgcga ccagaaggtg
     5041 tcgttgacaa agcgcccgta gctgccgttg ctcatgcgca ggaacgaggt gaaggcgaag
     5101 ccggccttca ggccattgcc caggtcttcg gtgccggtca cgccaaacca cgaagtggtc
     5161 atgccgttgt tgcccacggt gttcttgcgc tcggcgtcgc cggccatgcg catggagccc
     5221 acgtacatgt cggccaggcc ggacagttgt accgaacttt gcgcgtgcgc caacggcgcc
     5281 agcagcgaca ggccagccag acaggccggc acggcaaagg attttttcag catggattcc
     5341 ctcttttaaa acgactctga tctcaaacgc gggccccgga tcggtgcacg cacgcgcgaa
     5401 ccaggcacac gcggcacatg gcagcaacat tcatgccatg ccagtgcgta ttccgcagca
     5461 ccgtgtctgc caagaattta ataaaaaatg cctccagcgc atgtctgaca agcgcgggcc
     5521 gctaccaaaa atatagtgtt gtggcattga ttcaacacca ccgccaggcg cggtctgatc
     5581 gcattgcggc atgcacgcag tacgcgctgc gctcggctcg aaccgacgca gattcaggcg
     5641 caagcgggca agccccttcg cctgaagtcg ctcactccac cgtcacgctc ttcgccagat
     5701 ttctgggctt gtccacatcc gtcccccgcg ccaccgccgt gtgataagcc agcaactgca
     5761 gcggcaccac gtgcaggatg ggcgacagcg ggccgtagtg gccgggcatg cggatcacgt
     5821 gcaggccatc ggccgactgg atgtgcgtgt cgtcgtcggc cagcgcgtac agcacgccgc
     5881 cgcgcgcgcg cacttcctgc atgttgcttt tcagcttttc cagcaacgcg tcgtttggcg
     5941 ccacggtgac caccggcatg ctgctggtga ccagcgccag cgggccgtgc ttgagttcgc
     6001 cggcggcata cgcctcggcg tggatgtagg tgatttcttt cagcttcagc gcgccttctt
     6061 gcgccaccgg gtagtgcagg ccgcggccga ggaacagcgc gttctcttta ccggcaaact
     6121 gctcggccca ggtgatgatt tgcggctcca gcgccagcac ggcctgcagc gcgctgggca
     6181 ggtggcgcat ggctttcagg tggtcgtgct cctgctcttg ggtcagcagc ccgcgtgatt
     6241 tggcgagcgc cagcgtcagc aaaaacaggc cggcgagttg cgtggtgaag gccttggtgc
     6301 tggccacgcc gatctcgatg ccggcgcgcg tgatgtaggc cagcttgcat tcgcgcacca
     6361 tggcgctggt ggcgacgttg cacaccgtca gcgtgtgccg catgcccagc gattgcgcgt
     6421 ggcgcagcgc cgacagcgtg tcggcggttt cgcccgactg cgtgatggtg acgaccagcg
     6481 tggccgggtc gggcaccgat tcgcggtagc ggtattcgct ggcgatctcg acctggcagg
     6541 gcagcttggc gatggattcg atccagtatt tggccacgca acccgagtag tagctggtgc
     6601 cgcaggccag gatcagcacc tggcgaatcg actggaacgt ggggtaggcg ccctcgcccg
     6661 gcgcgtggtt gttggccgcc tcgaacagct cgggcacgat ggcctcgacg ccttccagcg
     6721 tgtcggcaat ggcgcgcggc tgctcgaaaa tttccttttg catgtagtgg cggtacgggc
     6781 ccagctcggc cgcgccgctc atggcctgca cggtcttgac ggggcgctgc acgc
//
LOCUS       DAOMVO010000116         6816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3009_length_6816_cov_16.883449, whole genome shotgun sequence.
ACCESSION   DAOMVO010000116 DAOMVO010000000
VERSION     DAOMVO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_6816_cov_16.883449"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /gene="mreD"
                     /locus_tag="PKV30_07105"
     CDS             <1..512
                     /gene="mreD"
                     /locus_tag="PKV30_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRL30017.1"
                     /translation="PRGQPLLLPASPAFIWGSLLVALMLNLLPLGRVLWMPDFLAVVL
                     VFWNIHQPRRIGLGVAFVFGLLLDVHQASLLGQHALAYTVLIYCAIMIHRRILWFSVP
                     GQALQLLPLFALTHAITIVLRLIGGAVFPGWPVLLSPVLEAALWPIVSVLLLAPQRRA
                     PNPDANRPL"
     gene            776..2734
                     /gene="mrdA"
                     /locus_tag="PKV30_07110"
     CDS             776..2734
                     /gene="mrdA"
                     /locus_tag="PKV30_07110"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRL30018.1"
                     /translation="MTEIRNVEADLVRFRVRLAVVALGVVAAFGLLAWRLYTLQVLRH
                     EELAQRAETNRTAVVPIVPHRGEILDRNGVVLATNYKAYTLELTPSRLGRTTDEAIDA
                     IAQVVEVTPRDRRRFKRLQEDSRNFESLPIRMRLSDEEVARFAAQRYRFPGVEIKARL
                     FRTYPMGETGAHVVGYIGRINQREKERIEDSDDAANYRGTDYIGKLGVESSYEKVLHG
                     TTGVEQMETSAGGYAVRRLASHPSTPGDSVRLSIDIQLQKLIEEMYGERRGALVAIDP
                     QNGEILAFVSKPTYDPNLFVEGIDQETWDELNNSLDKPLLNRAIRGTYPPGSTYKPFM
                     ALAALETGKRSTGTTIMDGGSWTFAGHTFRSGHALGAVDLHRSIVKSSNVYYYQLAND
                     MGVNAIHDFMKPLGFGQVTGVDIPGETRGVLPSTDWKRATYKRPEQQKWYGGETISLG
                     IGQGYNNFTMLQLANATATLAAGGVHHVPHLVKARQDPLSLQFKDLPLAPPENLGYKP
                     ENVAAVKRGMVGVTQEGTSRGVMAGAAYLSGGKTGTAQAVTIGQKSRYNAARLSEYQR
                     DHSLYIAFAPADNPRIAIAAIVENAGFGAAHAAPLVRRVMDYWLAGVYPSEADIAALQ
                     RGAATAPIGTPRRVADVVPAQPGAAAAP"
     gene            3017..4546
                     /locus_tag="PKV30_07115"
     CDS             3017..4546
                     /locus_tag="PKV30_07115"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRL30019.1"
                     /translation="MNDDTKKCPVTHLTTDFGAPVPTNRNSLTAGPRGPLLAQDVWLN
                     EKLANFVREVIPERRMHAKGSGAFGTFTVTNDITQYTRAKIFSEVGKKTEMFARFTTV
                     AGERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFIRDPRQFPDLNKAVKRDPRT
                     NLRSARNNWDYWTLLPEALHQVTIVMSDRGIPKSYRHMHGFSSHTYSFWNKDGQRFWV
                     KLHFRTQQGIANLTDAESAELIGKDRESHQKDLFEAIEKGDFPKWKMYIQVMPEVEAE
                     KYRLHPFDLTKVWYKGDYPLIEVGEFELNKNPENFFADVEQSAFAPSNLVPGISVSPD
                     KMLQARLFNYADAQRYRLGVNHQQIPVNRARCPVHSNHRDGQGRVDGNYGGLPHYEPN
                     SFGQWQAQPEFMEPPLKIHGDAAFWDQHQDDTNYFEQPGKLFRLMKPEQQEALFGNTG
                     RAMGDAPEFIKLRHIRNCNAADPAYGAGVAKALGIDLAKALASKEADPMFGNPLVPLP
                     A"
     gene            complement(4621..>6816)
                     /locus_tag="PKV30_07120"
     CDS             complement(4621..>6816)
                     /locus_tag="PKV30_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL30020.1"
                     /translation="AVRAALAELRAAHPDLTFTEAFDFVTPVEEEYHASLKMLGEGAL
                     LAVLVVWLFLRNGRATLVSAVALPMSILPALIGMYFFGFSINVVSLLALSLVIGILVD
                     DAIVEVENIVRHLRMGKSPYQAAMEAADEIGLAVIATTFTLIAVFLPTAFMSGVVGKF
                     FKQFGWTASLAVFASLVVARMLTPMMAAYLMKPLAQGGEDPRWLRVYGRWAAWCVRHR
                     FITLLGALAFFVGSLALVPLLPTAFITPDDNSQTQVYLELPPGATLQETEQTAEAARA
                     LLLKNQYIKSVYTTVGGGSAGGDPFSASSADVRKATLTIQLAERGDRPRKQIVEAELR
                     QLISGLPGVRSKIGLGGSGEKYILVLQGDDPHALTQAARAVESDLRTLPGLGSITSTA
                     SLVRPEIAVKPDFARAADLGVTSSAIADTLRVATLGDYDAQLPKLNLPQRQIPIVVKL
                     DADARKDLGVLSRLHVPGSKGPVPLSQVASVSLSGGPAVINRYDRARNVNLEVELSGQ
                     PLGDVAAKAKELPAVKQLPAGVRVVEVGDAEVMGELFMGFALAMLTGILCIYLVLVLL
                     FHHVLHPVTILAALPLSLGGAFVGLLLAHKSLSMPSLIGLIMLMGVATKNSILLVEYA
                     ILARREHGLKRFEALMDACHKRARPIIMTTLAMGAGMLPIAIGWGGSDPSFRSPMAVA
                     VIGGLITSTFLSLLVIPAVFTYVDDFAEWVKRRWHRGGHGAPASMPQQS"
BASE COUNT         1246 a         2384 c         2159 g         1027 t
ORIGIN      
        1 tgccgcgcgg ccaaccgctg ctgctgccgg ccagccccgc cttcatctgg ggcagtctgc
       61 tggtggcgct gatgctgaac ctgctgccgc tcgggcgcgt gctgtggatg cccgactttc
      121 tggccgtggt gctcgtgttc tggaacatcc accagccgcg ccgcatcggc ctgggcgtgg
      181 ccttcgtgtt cggcctgctg ctcgacgtgc atcaggcctc gctgctcggc cagcacgcgc
      241 tggcctacac ggtgctgatc tactgcgcga tcatgattca ccgccgcatc ctgtggttct
      301 cggtgccggg gcaggcgctg cagctgctgc cgctgttcgc gctgacgcac gccatcacca
      361 tcgtgctgcg gctgatcggc ggcgcggtgt ttccgggttg gccggtgctg ctgtcgccgg
      421 tgctcgaagc cgcgctgtgg cccatcgtca gcgtgctgct gctggcgccg cagcggcgtg
      481 caccgaatcc cgatgcgaat agacctctgt aaccccctga gtcgccttcg gcgccttccc
      541 ccaaggggga caacgctggc gctcgggggg accccgagcg cggcgttccg gggcggcctg
      601 ctccgcggcc attggggggg cttgctgcgc agcccgtgcg agcggttcgt gcaactccgc
      661 agggggtttt gactcgaatc gtttgccatg caccgttctt tgatcgaaaa tcgccactgg
      721 cgcttttggg acaagcgcga gcagctattt atttgatagt gtttcacgtt tcaccatgac
      781 cgaaatccgc aacgtcgaag ccgatctggt gcgctttcgc gtgcggctgg cggtggtcgc
      841 gctgggcgtg gtggcggcgt tcgggctgct ggcctggcgg ctgtacacgc tgcaggtgct
      901 gcggcacgaa gaactggcgc agcgcgccga gaccaaccgc acggcggtgg tgccgatcgt
      961 gccgcaccgc ggcgaaatcc tcgaccgcaa cggcgtcgtg ctggccacca actacaaggc
     1021 ctacacgctg gaactcacgc cgtcgcgcct gggccgcacg accgacgagg cgatcgacgc
     1081 catcgcgcag gtggtcgagg tcacgccgcg cgaccgccgc cgcttcaagc gcctgcagga
     1141 ggattcgcgc aacttcgaat cgctgccgat ccgcatgcgc ctgtccgacg aagaggtggc
     1201 gcgctttgcc gcccagcgct accgctttcc gggcgtcgag atcaaggcgc gcctgttccg
     1261 cacctacccg atgggcgaga ccggcgcgca cgtggtcggc tacatcggcc gcatcaacca
     1321 gcgcgaaaaa gagcgcatcg aggattccga cgacgccgcc aactaccgcg gcaccgacta
     1381 catcggcaag ctgggcgtgg agagcagcta cgagaaggtg ctgcatggca ccaccggcgt
     1441 cgagcagatg gaaacctcgg ccggcggcta cgcggtgcgc cggctggcca gccacccgtc
     1501 gacgccgggc gattcggtgc ggctgtcgat cgacatccag ctgcaaaagc tcatcgaaga
     1561 gatgtacggc gagcggcgcg gcgcgctggt cgccatcgac ccacagaacg gtgagatcct
     1621 ggccttcgtc agcaagccca cgtacgaccc caacctcttt gtcgaaggca tcgaccaaga
     1681 aacctgggac gagctgaaca actcgctcga caagccgctg ctcaaccgcg cgatccgcgg
     1741 cacttacccg cccggctcca cctacaagcc gttcatggcc ctggccgcgc tggaaaccgg
     1801 caagcgcagc accggcacca ccatcatgga cggcggctcg tggacgtttg ccggccacac
     1861 cttccgctcg ggccacgcgc tgggcgcggt cgatctgcac cgcagcatcg tcaagtccag
     1921 caacgtgtac tactaccagc tggccaacga catgggtgtc aacgccatcc acgacttcat
     1981 gaagccgctg ggttttggcc aggtcaccgg cgtcgacatc ccgggcgaaa cgcgcggcgt
     2041 gctgccttca accgactgga agcgcgccac ctacaagcgg cctgagcagc agaagtggta
     2101 cggcggcgag accatctcgc tcggcatcgg ccagggctac aacaacttca ccatgctgca
     2161 gctggccaac gccacggcca cgctggcggc gggtggcgtg caccacgtgc cgcacctggt
     2221 caaggcgcgg caagacccgc tcagcctgca gttcaaggac ttgccgctgg cgccgcccga
     2281 gaacctgggc tacaagcccg agaacgtggc cgccgtcaag cgcggcatgg tcggcgtcac
     2341 gcaggaaggc acctcgcgcg gcgtgatggc gggcgcggcc tacctgtcgg gcggcaagac
     2401 cggcaccgcg caggccgtca ccatcggcca gaagagccgc tacaacgccg cgcgcctgtc
     2461 tgaataccag cgcgaccact cgctttacat cgcctttgcg ccggccgaca acccgcgcat
     2521 cgccatcgcc gcgattgtcg agaacgccgg ctttggcgcc gcccacgccg cgcccctggt
     2581 gcgccgggtg atggattact ggctggccgg cgtctacccg agcgaagccg acatcgccgc
     2641 cctgcagcgc ggcgccgcca ccgcgcccat cggcacgccg cgccgcgtgg ccgacgtggt
     2701 gcccgcgcag ccgggcgccg ctgccgcgcc ttgacatagg ccatgaccgg cgctggcgcg
     2761 ccatgatctt gctaccgctc aatgacggcg cctgcgggcg ccatgaccca agtcatgcgc
     2821 ccacgggtgc gtcatccagg ccgcaggcct ggtcatgcgc gccagcgcgc agtcgtgcgg
     2881 ggcgtgtttg aaaacgccta ttcaccccat aaaaacgatt gacgtgaact tggcatgggg
     2941 ctcgcctaaa atggccgcgt ggggcgcctg cggcctcagc gatttttgtg tccacccacc
     3001 cccgaaggag cccccgatga acgacgacac caagaagtgc cccgtgaccc acctcacgac
     3061 cgacttcggc gcccccgtgc ccaccaaccg caacagcctc accgccggcc cgcgcggccc
     3121 gctgctggcg caggacgtgt ggctgaacga gaagctggcc aacttcgtgc gcgaggtgat
     3181 tcctgagcgg cgcatgcacg ccaagggctc cggcgcgttc ggcaccttca cggtgacgaa
     3241 cgacatcacc caatacaccc gcgccaagat cttcagcgaa gtcggcaaga agaccgagat
     3301 gttcgcccgc ttcaccaccg tggccggcga gcgcggcgcg gccgatgccg agcgcgacat
     3361 ccgcggcttt gcgctcaagt tctacaccga agaaggcaac tgggacatgg tgggcaacaa
     3421 cacgcccgtg ttcttcatcc gcgacccgcg ccagttcccc gatttgaaca aggccgtgaa
     3481 gcgcgacccg cgcaccaacc tgcgctcagc ccgcaacaac tgggactact ggacgttgct
     3541 gcccgaagcg ctgcaccagg tcaccatcgt gatgagcgac cgcggcatcc ccaagagcta
     3601 ccgccacatg cacggcttca gctctcatac gtacagcttc tggaacaagg acggccagcg
     3661 cttctgggtc aagctgcact tccgcacgca gcagggcata gcgaacctga cggatgccga
     3721 atcggccgag ctgatcggca aggatcgcga gagccaccag aaagacctgt tcgaggccat
     3781 cgaaaaaggc gacttcccca agtggaagat gtacatccag gtcatgcccg aagtcgaagc
     3841 cgagaagtac cgcctgcacc cgttcgattt gaccaaagtt tggtacaagg gcgactaccc
     3901 gctgatcgaa gtcggcgagt tcgagctgaa caagaacccc gagaacttct ttgccgatgt
     3961 cgagcaaagc gccttcgccc ccagcaacct ggtgcccggc atcagcgtca gccccgacaa
     4021 gatgctgcag gcgcgcctgt tcaactacgc cgacgcgcag cgctatcgcc tgggcgtgaa
     4081 ccaccagcag attccggtca accgcgcgcg ctgcccggtg cacagcaacc accgggacgg
     4141 ccagggccgc gtcgacggca actacggcgg cctgccgcac tacgagccca acagctttgg
     4201 ccagtggcag gcgcagcccg agttcatgga gccgccgctc aagatccacg gcgacgccgc
     4261 cttctgggac cagcaccagg acgacaccaa ctacttcgag caaccgggca agctgttccg
     4321 cctgatgaag ccggagcagc aagaagccct gttcggcaac accggccgcg ccatgggcga
     4381 cgcgcccgag ttcatcaagc tgcgccacat ccgcaactgc aacgcggccg atccggccta
     4441 cggcgcgggc gtggcgaaag cgttggggat tgatctggcg aaggcgctgg cgtcgaagga
     4501 agccgacccc atgttcggca acccgctggt gccgctgcca gcgtaatttg tggatgaaat
     4561 aggctgctgg cgcttgtctg tcaagcgcca gcagctattc aaaaaatagc gaacagcttg
     4621 tcaagattgc tgcggcatcg acgccggcgc gccatgacca ccgcggtgcc agcgccgttt
     4681 cacccattcg gcgaagtcat cgacataggt aaacaccgcc ggaattacca gcaggctcaa
     4741 gaaagtggag gtgatcaacc ccccaatcac cgccaccgcc atcggcgagc gaaagctggg
     4801 gtcggagccg ccccagccga tggcaatggg cagcatgccg gcgcccatgg cgagcgtggt
     4861 catgatgatg gggcgcgcgc gcttgtggca cgcatccatc agggcctcga agcgtttcag
     4921 cccgtgctcg cgccgcgcca ggatggcgta ctcgaccagc aagatcgagt tcttggtcgc
     4981 cacgcccatc agcatgatca aaccgatcag cgagggcatc gacagcgact tatgcgccag
     5041 cagcagcccg acaaacgcgc cgcccagcga cagcggcagc gccgccagaa tcgtgaccgg
     5101 gtgcagcacg tggtggaaca gcagcaccag caccaggtag atgcacaaaa tgcccgtcag
     5161 catggccagc gcaaagccca tgaacagctc gcccatcacc tcggcgtcgc ccacctcgac
     5221 cacgcgcacg ccggccggca gttgcttgac ggcgggcaac tccttggcct tggccgccac
     5281 gtcgcccagc ggctggcctg aaagctcgac ctccaggttg acgttgcgcg cgcggtcgta
     5341 gcggttgatg acggcgggcc cgcccgacag gctgaccgac gccacctgcg acaacggcac
     5401 cggccccttg ctgcccggca cgtgcaggcg cgacagcacg cccagatcct tgcgcgcatc
     5461 ggcatcgagc ttgacgacga tggggatctg ccgctgcggc agattcagct tgggcagttg
     5521 cgcgtcgtag tcgcccagcg tggccacgcg cagcgtgtcg gcgatggcgc tgctggtcac
     5581 gcccaggtcg gcggcgcgcg cgaagtcggg cttgacggca atctcgggcc gcaccaggct
     5641 ggccgtgctg gtgatgctgc cgagacccgg cagcgtgcgc aaatcgctct cgaccgcgcg
     5701 cgcggcctgc gtcagcgcgt gcgggtcgtc gccttgcagc accaggatgt acttctcgcc
     5761 cgagccgccc aggccaatct tgctgcgcac gccgggcaag ccgctgatga gctggcgcaa
     5821 ctcggcttcg acgatctgct tgcgcggccg atcaccgcgc tcagccagct gaatggtgag
     5881 cgtggccttg cgcacatcgg cactgctggc cgaaaacgga tcgccgccgg ccgagccgcc
     5941 gcccacggtg gtgtacacgc tcttgatgta ttggttcttg agcagcagcg cgcgcgccgc
     6001 ctcggccgtc tgctctgttt cctgcagcgt cgcgccgggc ggcagctcca gatagacctg
     6061 cgtttgcgag ttgtcgtcgg gcgtgatgaa ggcggtgggc agcagcggca ccagcgccag
     6121 cgagccaacg aagaaggcca gcgcgcccag cagcgtgatg aagcgatgcc gcacgcacca
     6181 cgccgcccag cggccgtaca cacgcagcca gcgcgggtct tcgccgcctt gcgccagcgg
     6241 cttcatcaaa tacgccgcca tcatcggcgt cagcatgcgc gccaccacca gcgaggcaaa
     6301 caccgccagc gacgccgtcc agccgaactg cttgaagaac ttgccgacca cgccgctcat
     6361 gaaggcggtg ggcaagaaca ccgcaatcag cgtgaaggtg gtggcaatca ccgccaggcc
     6421 aatctcgtcg gccgcctcca tcgccgcctg gtacggcgac ttgcccatgc gcaggtggcg
     6481 cacgatgttc tcgacctcga cgatggcgtc gtccaccaga atgccgatga cgagcgacag
     6541 cgccagcagc gacaccacgt tgatcgagaa gccgaagaaa tacatgccga tcagcgccgg
     6601 caagatcgac atcggcagcg ccaccgccga cacaagggtc gcgcgcccgt tgcgcaagaa
     6661 cagccacacc accagcaccg ccagcagcgc gccttcgccc agcatcttga gcgaggcgtg
     6721 gtattcctct tccaccggcg tgacgaaatc gaacgcttcg gtaaaggtca ggtcggggtg
     6781 cgcagcgcgc agttcggcca gcgcggcgcg cacggc
//
LOCUS       DAOMVO010000117         6752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3038_length_6752_cov_15.364193, whole genome shotgun sequence.
ACCESSION   DAOMVO010000117 DAOMVO010000000
VERSION     DAOMVO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_6752_cov_15.364193"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKV30_07125"
     CDS             complement(<1..225)
                     /locus_tag="PKV30_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRL30021.1"
                     /translation="MPVWRQYYVLTKPRVVQLIVFCALIGMVLAVPGLPDWAALQRML
                     VACAGIWLVAGAAAAFNCLIEKTIDAKMKRT"
     gene            complement(342..1583)
                     /locus_tag="PKV30_07130"
     CDS             complement(342..1583)
                     /locus_tag="PKV30_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRL30022.1"
                     /translation="MGRFARRKGVASVTEQALYDLSPALRLMGMGLVVAMGPLAWLWL
                     RGRGQSPARRLQALTLLTLFLTFDLVLFGAFTRLTDSGLGCPDWPGCYGKASPVGARE
                     HIAAAQQAMPTGPVTHGKAWVEMVHRYLATGVGVLIIVLALATWLQWRRSRVAQGQRA
                     DGSASIVHPGWPLFTLLWVCAQGAFGALTVTMKLFPAIVTLHLLGGVGLLVLLAAQAE
                     RYRQAAGAPAELLGHGTRTLLWAATLMVGVQIALGGWVSTNYAVLVCNTFPMCQGSWW
                     PAMDFAQGFELWRPLGTSADGSPLEFHALTAIHYTHRLFAYAVFALLSGLAWRLHQQG
                     RLARQRRWIVGLAALQLVTGLSNVVLDWPLVAAVLHTGGAAALMTVLTWALCASRAAS
                     QFDSKTIAARAIPTSAGGLKT"
     gene            complement(1687..2322)
                     /locus_tag="PKV30_07135"
     CDS             complement(1687..2322)
                     /locus_tag="PKV30_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30023.1"
                     /translation="MPSPGGAALQDERRTRAGRWKMLLLALVCAAPVIASYLSYYVLR
                     PGARTGFGELIEPQRPIPAVAATDLNGQPFDLPALRGQWLLVSVSGGACDADCQNNLY
                     LQRQLRESMGKNKDRIDWLWLVGDDARVPDSLRPGLSQATVVRVPADALARWLAPAAG
                     HQLSEHLYVVDPMGHWMMRFPAHLDKAGAGKAKRDLDRLLRASASWDQAGR"
     gene            complement(2366..3046)
                     /locus_tag="PKV30_07140"
     CDS             complement(2366..3046)
                     /locus_tag="PKV30_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRL30024.1"
                     /translation="MALTASLGRWQLSRAAEKEALQAAIAERQRLPALDGASLAQALS
                     DDQARELLHRAITLRGQWLADRTVFLDNRPMARQTGFYVLTPLRLTGSDRVVLVQRGW
                     APRHQVDRSLLPPVQTPTGAVQVSGRIAAHPPRVYQLGQEQGGAIRQNLDLDAWRAET
                     GLPLAPVLVVQTGAASDGLQRDWPEAASGVEKHYGYAFQWFGLCTLIGLLLLWFQVVR
                     PLYRARRA"
     gene            3113..3358
                     /locus_tag="PKV30_07145"
     CDS             3113..3358
                     /locus_tag="PKV30_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRL30025.1"
                     /translation="MRAMKQVLFALAFAGILGSLASALVFMLKRDKPGEKPDAERASR
                     MMRALALRVGLSVLLFICVLVAWKLGYIRPTGISAGA"
     gene            complement(3435..4316)
                     /locus_tag="PKV30_07150"
     CDS             complement(3435..4316)
                     /locus_tag="PKV30_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRL30026.1"
                     /translation="MSTTAHGHTPYYYVPAESRHPVMAAIGLFFMILGAGMWVNGAGW
                     APYAFFFGMAWWLFVLYQWFGDAIRESEGGLYSRKIDLSYRWSMSWFIFSEVMFFGAF
                     FTALWWARAHSVPALGSLENALLWPDFKAVWPSLAAGATGSPAGIVEPFQTMGPFWLP
                     TINTALLLTSGVTLTIAHHALLANHRSRCITFMWITVILGATFLCVQGYEYAHAYSDL
                     NLKLSSGVYGSTFYMLTGFHGFHVLVGMLMLLFITLRLMKGHFTPERHFGFEGAAWYW
                     HFVDVVWLGLFILVYWM"
     gene            complement(4407..4637)
                     /locus_tag="PKV30_07155"
     CDS             complement(4407..4637)
                     /locus_tag="PKV30_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HRL30027.1"
                     /translation="MNPSSESAPPTKPSFLRSLRLVAWGFLGVRKNSEYQRDLAQVNP
                     FHVILAGIIGVLLLVLLLVGLVNWAVSGAPAG"
     gene            complement(4634..5248)
                     /locus_tag="PKV30_07160"
     CDS             complement(4634..5248)
                     /locus_tag="PKV30_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRL30028.1"
                     /translation="MRLRTENLRMVVKLAVVVTGMFAFGYALIPLYRAICEATGINIL
                     SLSERQVPGNGTAGVAARSAMRNTQVDRTRVITVEFDANSRGPWEFKPAQRSIQVHPG
                     ELATVMYEFQNVQPRRMAAQAIPSYAPRQAAPHFNKLECFCFTQYTLDPGERKQWPVA
                     FIIDPRISKDVTTITLSYTFFEVGGKTPAAPLASAKSMPTGVGT"
     gene            complement(5274..5381)
                     /locus_tag="PKV30_07165"
     CDS             complement(5274..5381)
                     /locus_tag="PKV30_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HRL30029.1"
                     /translation="MTTEEQRKRNVRLGLILGSVAVVFFVGFIVRMALL"
     gene            complement(5404..>6752)
                     /locus_tag="PKV30_07170"
     CDS             complement(5404..>6752)
                     /locus_tag="PKV30_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HRL30030.1"
                     /translation="PAFVGFANWMIPLQIGASDMAFARMNNFSFWLLIPAGIMLVGSF
                     FMPGGAPAAGWTLYAPLTLQMGMSMDTSIFAMHIMGASSIMGAINIIVTILNMRAPGL
                     TLMKMSMFCWTWLITAYLLIAVMPVLAGAITMTLTDRHFGTSFFNPAGGGDPIMYQHI
                     FWFFGHPEVYIMILPAFGIVSQVVPAFARKRLFGYASMVYATSAIAILSFIVWAHHMF
                     TTGMPVTGQLFFMYATMLIAVPTAVKIFNWIATMWRGSMSFETPMLWAVGFIFVFTIG
                     GFTGLIPAVAPIDTQIQDTYYIVAHFHYVLVAGSLFAMFAGYYYWSPKWTGVMYNETR
                     GKIHFWWSMIAFNLTFFPMHFLGLAGMPRRYADYPMQFADFNMIASVGAFFFGFAQVY
                     FFLFVVVPMMRGQGAKAPQRPWEGAEGLEWEVPSPAPFHTFENPPKLDATATRVIG"
BASE COUNT         1268 a         2232 c         2257 g          995 t
ORIGIN      
        1 ggtgcgcttc atcttggcgt cgatggtttt ttcgatcagg cagttgaagg ccgccgccgc
       61 accggccacc agccagatgc cggcgcaggc gacaagcatg cgctgcaggg ccgcccagtc
      121 cggcaggcct ggcacggcga gcaccatgcc gatgagggcg cagaagacaa tcagttgcac
      181 cacgcgcggc ttggtcagca cgtagtactg gcgccagacg ggcatggggg gaagcgcgcc
      241 cccacgctcc gccgctgcgc gcgctgcgct gccccccgag ggggctgccc cgccttgggg
      301 cggcccggcg gcggggcgga cggtgtcggg tgcgctggtc ttcatgtttt caggccccca
      361 gcgcttgtgg ggattgcgcg agcagctatc gtttttgaat caaactgcga tgccgcgcgg
      421 ctggcgcaca aggcccaagt cagcaccgtc atcagcgccg ccgcaccgcc ggtgtgcagc
      481 acggcggcga ccagtggcca gtcgagcacc acgttcgaca ggccggtaac cagttgcagc
      541 gccgccaggc ccacgatcca gcggcgctgg cgcgcgaggc ggccctgctg atgcagacgc
      601 caggccaggc cgctcagcag ggcgaacacg gcgtaggcga acaggcgatg cgtgtagtga
      661 atggcggtga gcgcgtgaaa ttcaagcggc gaaccatcgg ccgaggtgcc cagcgggcgc
      721 cacagctcaa agccctgcgc aaagtccatg gcgggccacc aactgccctg gcacatcggg
      781 aaggtgttgc acaccagcac cgcgtagttg gtgctgaccc agccgcccag cgcaatctgc
      841 acgcccacca tcagcgtggc ggcccacagc agcgtgcgcg tgccatggcc cagcaattcg
      901 gccggcgcgc cggcggcttg tcggtaccgc tcggcctgcg ccgccagcag caccaacaga
      961 cccacgccgc ccagcaggtg cagcgtgacg atggcaggga acagcttcat cgtcacggtg
     1021 agcgcaccga aagcgccctg cgcgcagacc cacagcaggg tgaacagggg ccagcccggg
     1081 tgcacgattg aggcgctgcc atccgctcgc tgaccttggg cgacgcgact gcgccgccac
     1141 tgcagccagg tcgccagcgc cagcacgatg atcagcacgc cgaccccggt ggccaggtag
     1201 cggtgaacca tctccaccca ggccttgccg tgcgtgaccg ggccggtcgg catggcttgc
     1261 tgcgcggcgg cgatgtgttc gcgcgcgccc acggggctgg ccttgccgta gcagcccggc
     1321 cagtcggggc agcccaggcc cgaatcggtc aggcgcgtga aggcgccgaa cagcaccagg
     1381 tcgaaggtca agaacagcgt gagcagggtc aacgcctgca ggcggcgcgc cggcgactgc
     1441 ccgcgcccgc gcagccacag ccaggccagc ggccccatgg ccaccaccag ccccatgccc
     1501 atcaggcgca gcgcgggcga gaggtcgtac agggcttgtt cggtcaccga ggccacaccc
     1561 ttgcgccgcg cgaagcgccc catggccgcg cagcaggcca cgccggcacg ccgtgccgcg
     1621 ggcttggccc ggccactggc gttgctcccc tggggggacg gtaccgcagg cgactcagaa
     1681 ggcggctcat cttcctgcct gatcccagct ggccgacgcg cgcagcaagc gatccagatc
     1741 gcgcttggcc ttgccggcac cggctttgtc gaggtgcgcg ggaaagcgca tcatccaatg
     1801 ccccatcggg tcgaccacgt acagatgctc cgacagttgg tgccctgcgg ccggcgccag
     1861 ccagcgcgcc agcgcatcgg ccggcacgcg caccacggtg gcctggctga ggcctgggcg
     1921 caggctgtcg ggcacgcgcg cgtcgtcgcc gaccaaccag agccagtcga tgcggtcctt
     1981 gttcttgccc atgctctcgc gcaactgcct ctgcaggtac agattgttct ggcaatccgc
     2041 gtcgcaagcg ccaccggaca cgctgacgag cagccactgg ccgcgcagag cgggcagatc
     2101 gaacggctgg ccgttgagat cggtggcggc gacagcaggg atggggcgct gcggctcgat
     2161 cagttcgcca aagccggtgc gagcgcccgg gcgcagcacg taatagctga ggtacgaggc
     2221 gatcaccggc gcggcgcaca ccagcgccag cagcagcatc ttccagcggc cggcgcgcgt
     2281 gcggcgctcg tcctgcagcg cggcaccgcc gggtgagggc atgtcgtgca ccgtcaggcc
     2341 aagcggccgg tcgaggtgat gaggttcagg cgcggcgggc gcggtagaga ggtctgacaa
     2401 cttgaaacca cagtaaaagc aggccgatca acgtgcacag accgaaccac tggaaggcgt
     2461 agccgtagtg tttttcgacg ccggaagccg cctcgggcca gtcgcgttgc aagccgtccg
     2521 aagcggcgcc ggtctgcacc accagcacgg gggccagcgg caaaccggtt tcggcacgcc
     2581 acgcgtccag gtcgagattt tgccggatcg cgccgccctg ttcctgaccg agttgataga
     2641 ccctgggcgg gtgtgctgcg atgcggcccg acacctgcac cgcgccggta ggcgtctgca
     2701 cgggcggcag caagctgcga tcgacctggt ggcgcggcgc ccagccgcgc tgcaccagca
     2761 ccacccggtc actgcccgtc aggcgcagcg gcgtcagcac gtaaaaaccg gtctggcgcg
     2821 ccatgggccg gttgtcgaga aagaccgtgc ggtcggccaa ccattggccg cgcagggtga
     2881 tggcgcggtg caaaagctcg cgcgcctggt cgtcggacaa cgcttgggcc aagctggcgc
     2941 cgtccagcgc cggcaagcgc tggcgctcgg cgatggcggc ctgcaaagcc tctttctcgg
     3001 ccgcgcgcga cagctgccag cggccgagcg acgccgtcag cgccatggcc gccagcgcca
     3061 ccgcccacac cagcgcgcgg cgccaggccg ttgggattcg tctcacggga taatgcgggc
     3121 catgaaacaa gtgctgttcg cgctggcgtt tgcaggcatt ctgggcagcc tggcgtcggc
     3181 gctggtgttc atgctcaaac gcgacaagcc gggtgaaaaa ccggacgcag agcgtgccag
     3241 ccgcatgatg cgggcgctgg cgctcagggt cgggttgtcg gtgctgctgt tcatttgcgt
     3301 gctggtggcc tggaagctgg gctacatccg cccaacagga atttccgccg gcgcctgagg
     3361 gccacctgcc cacgcgccag aaaaaagagc gccgcgaggg cgctcttttc cttgtcgcaa
     3421 gctgcgaggc cccgtcacat ccagtacacc agaatgaaca ggcccagcca caccacgtcg
     3481 acgaagtgcc agtaccaggc cgcgccttca aagccgaaat ggcgctcggg cgtgaagtgc
     3541 cccttcatca ggcgcagcgt gatgaacagc agcatcagca tgcccaccag cacgtgaaag
     3601 ccgtgaaagc cggtcagcat gtagaaggtc gagccgtaca cgcccgagct gagcttgagg
     3661 ttgaggtcgc tgtaggcgtg ggcgtattcg taaccctgca cgcacaggaa ggtggcgccc
     3721 agaatcaccg tgatccacat gaaggtgatg cagcggctgc ggtggttggc cagcagcgcg
     3781 tggtgggcga tggtcagcgt gacgcccgaa gtcagcagca gcgcggtgtt gatggtcggc
     3841 agccagaacg ggcccatggt ctggaacggc tcgacgatgc cggccggcga tcctgtggcg
     3901 cctgccgcca agctgggcca cacggccttg aagtcgggcc acagcagcgc gttctccagg
     3961 ctgcccagcg ccggcaccga gtgcgcgcgc gcccaccaca aggcggtgaa gaaggcgccg
     4021 aagaacatca cctcggagaa gatgaaccag ctcatgctcc agcggtaaga gaggtcgatc
     4081 ttgcggctgt acaagccgcc ctcgctctcg cggatggcgt cgccgaacca ctggtagagc
     4141 acgaacagcc accaggccat gccgaagaag aatgcgtacg gcgcccagcc ggcgccgttc
     4201 acccacatgc cggcaccgag gatcatgaag aacaggccga tggcggccat caccgggtgc
     4261 cgcgattcgg ccggaacgta gtaatacggc gtgtggccgt gtgctgtggt ggacatcttc
     4321 acttcactcc tgtactctca atttaatagc tgcttgcgct tgttcagctt gcgccggagg
     4381 cctgtttggc ttgaaattca cccatttcat cccgccggcg caccggacac cgcccagttg
     4441 accagcccga ccagcaacag caccagcagc aacacgccga tgatgccggc caggatcaca
     4501 tgaaacgggt tcacctgcgc caggtcgcgc tggtattcgc tgttcttgcg cacgcccaga
     4561 aagccccacg ccaccaggcg cagcgagcgc aagaacgatg gcttggtcgg cggcgccgac
     4621 tcactcgacg ggttcatgtg cccaccccgg ttggcatcga cttcgcgctg gccagcggcg
     4681 ctgcgggcgt cttgccgccc acctcaaaga aggtgtacga caacgtgatg gtggtcacgt
     4741 ctttcgagat gcgcggatcg atgatgaacg ccaccggcca ctgcttgcgc tcgcccggat
     4801 cgagcgtgta ctgcgtgaag cagaagcact ccagcttgtt gaagtgcggc gccgcctggc
     4861 gcggcgcgta gctgggaatg gcttgcgccg ccatgcgtcg tggctgcacg ttctggaact
     4921 cgtacatcac cgtggccagc tcgcccggat gcacctgaat agaccgctgc gccggcttga
     4981 actcccacgg cccgcgcgaa ttggcgtcga actcgacggt gatgacgcgg gttcgatcga
     5041 cctgcgtgtt gcgcatggcc gagcgcgccg ccacgcctgc cgtgccattg cccggcacct
     5101 ggcgctcgga cagcgagagg atgttgatgc cggtggcttc gcagatcgcg cggtaaagcg
     5161 gaatcagcgc atagccgaag gcaaacatgc ccgtcacgac caccgcgagc ttgaccacca
     5221 ttctgagatt ttcggtgcgc aaacgcatcg cccaataccc ggtgcgtcag ccgctacagc
     5281 agcgccatgc gcacgatgaa gccgacgaag aacacgaccg cgaccgagcc caggatcagc
     5341 cccaggcgga cgttgcgctt gcgttgctct tcggtggtca tcatgccgcg ggcgaatgat
     5401 ccatcaaccg atcacgcggg tcgccgtggc gtccagcttg ggcgggttct cgaaggtgtg
     5461 gaacggtgcc ggcgacggca cttcccactc caggccttcg gcgccttccc acggacgctg
     5521 cggcgccttt gcgccctgac cacgcatcat cggcaccacc acgaacagga agaaatacac
     5581 ctgcgcgaag ccgaagaaga aggcacccac cgaggcgatc atgttgaagt cggcgaactg
     5641 catcgggtag tcggcataac ggcgcggcat gccggccaga cccaggaagt gcatcgggaa
     5701 gaacgtgagg ttgaacgcga tcatcgacca ccagaagtgg atcttgcccc gcgtctcgtt
     5761 gtacatcacg ccggtccact tgggtgacca gtagtagtag cccgcaaaca tggcgaacag
     5821 cgagccggcc accaacacgt agtggaagtg ggccacgatg tagtaggtgt cctggatctg
     5881 cgtgtcgatc ggcgccacag ccgggatcag gccggtgaag ccaccgatgg tgaacacgaa
     5941 gatgaaacca accgcccaca gcatcggcgt ctcaaacgac atcgagccac gccacatggt
     6001 ggcgatccag ttgaagatct tcacggccgt cggcaccgcg atcagcatgg tcgcgtacat
     6061 gaagaacagc tggcccgtga ccggcatgcc ggtggtgaac atgtggtgcg cccacacgat
     6121 gaagctcagg atggcaatgg ccgaggtggc gtacaccatc gacgcgtagc cgaacagccg
     6181 cttgcgcgcg aaggccggca ccacctggct gacgatgccg aaggccggca agatcatgat
     6241 gtagacctcg gggtgaccga agaaccagaa gatgtgctgg tacatgatcg gatcgccgcc
     6301 gccggcggga ttgaagaagc tggtgccgaa gtggcgatcg gtcagcgtca tggtgatggc
     6361 gccggccagc acgggcatca cggcgatcag caggtaggcg gtaatgagcc aagtccagca
     6421 aaacatggac atcttcatca gcgtcaggcc cggcgcgcgc atgttcagga tggtgacgat
     6481 gatgttgatc gcccccatga tcgagctggc gcccatgatg tgcatggcga agatgctggt
     6541 gtccatcgac atgcccattt gcagcgtcag cggcgcatac agcgtccagc ccgcagcggg
     6601 ggcgccgccc ggcatgaaga acgagcccac cagcatgatg ccggccggaa tcagcagcca
     6661 gaagctgaag ttgttcatgc gcgcgaacgc catgtcactg gcgccgatct gcagcgggat
     6721 catccagttc gcgaagccca caaaggccgg ca
//
LOCUS       DAOMVO010000118         6749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3040_length_6749_cov_14.307589, whole genome shotgun sequence.
ACCESSION   DAOMVO010000118 DAOMVO010000000
VERSION     DAOMVO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3040_length_6749_cov_14.307589"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKV30_07175"
     CDS             <1..231
                     /locus_tag="PKV30_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HRL30031.1"
                     /translation="VQQWLHAELEKPLDPAEVARAASTPEMAAEMYLASLLVADEQSF
                     MERAYLDELARQLKLEPALQQRLQNQLVTAGA"
     gene            441..935
                     /locus_tag="PKV30_07180"
     CDS             441..935
                     /locus_tag="PKV30_07180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL30032.1"
                     /translation="MGRPRMLWQINRMDAPVALPVPVQGVAARRIADTWHGPRDGGTR
                     RHEGTDIFAARGTPVRSSTVGIVAAIREGGIGGKQVWVLGPARQRHYYAHLDGWAPEL
                     ATGDVVQPGTLLGFVGTTGNAQGTPPHLHYGIYGAEGALNPWPLLQAGAAASTPGAAA
                     QPRR"
     gene            1033..3069
                     /locus_tag="PKV30_07185"
     CDS             1033..3069
                     /locus_tag="PKV30_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRL30033.1"
                     /translation="MTRPPHDPHPRALGALRGAWRRWALWGALVLVVAGLLATLVWLA
                     RGYEIEQVQRDLERDSADAAQALRQRLARNLQDLHSLATTPADPVGWWSAASDVLHEH
                     REWLRLQWRGPALRLQAAADSAFFPPLPPAAPDGEAAAAADIALACNLARKSGSAAYA
                     PSRYVPRPGGLGEEVMELCLPILNGAQVQGYVIATYTLEGLLTLLPPGLTRGQSVALT
                     ELDGTRLAVLGAPRRGTRVFVAQQVLDLPGAPMVLRLEGARQLPDVFPNVLTALVTGL
                     SIALVTVLALLAGDFRRRQRAEAELAEALAFRKAMEDSLVTGLRARDLEGRITYVNPA
                     FCAMVGYGADELIGRGAPAPYWPPERAHEYGERLALRYGGQASAREGVESVFMHQDGT
                     RFPVLIFEAPLLTSGGQQTGWMSAVMDVTAQRRAEESSRASHERLQASARLATVGEMA
                     SLMSHELNQPLAVIASYANGSLNLLQGASAADAHATLGDVRTALARIAEQAGRAGKVI
                     HSVRDLVRQRSTAREAVTPRALFDTVLPLVQLQARKLGATVQLEVPPELPAVWCEPTM
                     IEQVLLNLARNGLQAMDEVAGARVLRLQARALPVTALGFVSSVEFSVADTGGGISEAV
                     AAELFTPFFTTKAEGMGLGLSLCRTVIEQHGSALQHQPQSPSGTVFSFRLPLAR"
     gene            complement(3121..3531)
                     /locus_tag="PKV30_07190"
     CDS             complement(3121..3531)
                     /locus_tag="PKV30_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HRL30034.1"
                     /translation="MRALELYDYAVIRVVPRVEREEFVNAGVILSCQASGFLAAAIAL
                     DEARLQALDAGVDLPAVQRHLAAVVAICEGAPGSGPIGQLPLRARFHWLTARRSAIIQ
                     TSPVHTGRCTDGASALQHIMRRMVWPPEPRNLAK"
     gene            complement(3518..>4261)
                     /locus_tag="PKV30_07195"
     CDS             complement(3518..>4261)
                     /locus_tag="PKV30_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HRL30035.1"
                     /translation="GGSMPAIVEGDDDGLYVLKFRGAGQGVRALIAEILAGGIARALG
                     LNVPEIVLAELDVELARTEPDPEIQDLMRASGGLNVALDYLPGSANFDPAADQVDANT
                     ASRIVWFDALVGNVDRSARNTNLLVWHGKPWLIDHGATLTFHHGWQAPVADAGKPFAA
                     IADHVLLPAASALAQVDAELAARLTPDVLRGVVDEVPESFLALSAQDQPPGSALATAA
                     AQRQSYLHYFSARVADRARWLEGAERARA"
     assembly_gap    4262..4361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4469..5422)
                     /locus_tag="PKV30_07200"
     CDS             complement(4469..5422)
                     /locus_tag="PKV30_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30036.1"
                     /translation="MQVKDDPLQPRARAVLGQVSDMDLRLLQVFKAVVECGGLSAAEL
                     ELNIGTSTVSRHIKDLETRLGLTLCRRGRAGFALTAEGQRVYDETLRLLAAVDGFRAG
                     VDDIHQRMGGTLAVALFDKTASNPAARIHAAIDAFTRAAPDVQLHLHVGSIPAIERGV
                     MDGSFAIGVIPEHRASASLRYDRLFGETMHLYCGHDHALFNSERLALDWSALRARKDF
                     AGLGYHSPNMALAHGRRLPRAATAFEQEAIATLILSGRFVGFLPDHYAEAFVQRGQMR
                     AVNSALLHYACDFVSVLRRSPQPSRAAQAFHQCLLQAHGVN"
     gene            5528..>6749
                     /locus_tag="PKV30_07205"
     CDS             5528..>6749
                     /locus_tag="PKV30_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL30037.1"
                     /translation="MSVTATQPAAAHTAPRQDAAWLDAHWMPFTANRQFKRDPRMIVA
                     AQGAYFTDGDGRQVFDGLSGLWCTGLGHGRREIAEAVGKQAAQLDYSPGFQFGHPLSF
                     ELANKLKAFTPAGLDYVFFTASGSEAADTALKMARAYWRLKGQGGKQLLVGREKGYHG
                     VNFGGISVGGMVGNRKLYGTAVQADHLPHTQPPMGTFAKGQPAEGGKQLADRLLDLIA
                     LHDASNIAAVIVEPFSGSAGVVIPPVGYLQRLREICTQHDILLIFDEVITGFGRCGSR
                     TGADAFGVVPDIMTFAKQVTNGVQPLGGVIASKAIYDTFMAAGGPDYMLEFAHGYTYS
                     AHPVACAAGIAAMDLLEREDGVGRVKALAPHFEVAVHSLKGAKHVLDIRNYGLAAGLT
                     IAPAPGEPAKRPYEI"
BASE COUNT          994 a         2326 c         2342 g          987 t
ORIGIN      
        1 gtgcagcaat ggctgcacgc cgagctggaa aagccgctcg accccgccga agtcgcgcgc
       61 gccgccagca cgcctgaaat ggcggccgag atgtacctgg ccagcctgct ggtggccgac
      121 gaacaaagct tcatggaacg cgcctacctg gacgaactgg cgcgccagct caagctggaa
      181 ccggcactgc agcagcgcct gcagaaccag ctggtcactg ctggcgcttg agcgtgcggc
      241 gcgctgaatg gcggcgcttt tcgctattta ttggatagct tcttgcgctt gatggacaag
      301 cgctgagggc ctttttgatg cctgaacacc ggtcacccgc cgcgccacgc tgggcccacc
      361 gcctgcggcg cgcgctgacc ggcgcgctgt ggctggtggc gctgtggtgg ttcggcggct
      421 ggctgtggga ccagcccttc atgggccgcc cgcgcatgct gtggcagatc aaccgcatgg
      481 acgcccccgt cgcactgccg gtgccggtgc aaggcgtggc cgcgcgccgc atcgccgaca
      541 cctggcacgg cccgcgcgat ggcggcacgc ggcggcatga aggcaccgac atctttgccg
      601 cgcgcggcac gccggtgcgc agcagcaccg tgggcatcgt cgcggcgatc cgcgaaggcg
      661 gcatcggcgg caagcaggtg tgggtgctgg gcccggcgcg ccagcgccac tactacgcgc
      721 acctggacgg ctgggcgccc gagctggcca cgggcgacgt ggtgcagccc ggcaccctgc
      781 tcggctttgt cggcaccacc ggcaatgcgc agggcacgcc gccgcatttg cactacggca
      841 tttacggcgc cgaaggggcg ctgaacccgt ggccgctgct gcaggccggg gctgcggcgt
      901 cgacgccggg cgcggccgcc caacctcgcc gctaagccaa ccgcggcgcg cggtctgcgt
      961 ggaaaatcca ggctcgctgc ggtatccgca gccgccccca cccttgcccg cccaccccac
     1021 gcccgacccg cgatgacgcg cccaccccat gatccgcacc cgcgcgcgct gggcgcgctg
     1081 cgcggcgcgt ggcggcgctg ggcgctgtgg ggcgcgctgg tgctggtggt ggcggggctg
     1141 ctggcgacgc tggtgtggct ggcgcggggg tatgagatcg agcaggtgca gcgcgatctg
     1201 gagcgcgaca gcgccgatgc cgcgcaggcg ctgcgccagc ggctggcgcg caatctgcag
     1261 gatttgcaca gcctggccac cacgccggcc gacccggtcg gatggtggag cgcggcgtcg
     1321 gacgtgctgc acgagcaccg cgagtggctg cgcctgcagt ggcgcggccc ggcgctgcgc
     1381 ctgcaagccg cggccgacag cgccttcttt ccgccgctgc cgcccgccgc gccggatggc
     1441 gaagccgccg ccgcggccga tatcgcgctg gcctgcaacc tggcgcgcaa gagcggcagc
     1501 gccgcctacg cgcccagccg ctacgtgccg cgccccggcg ggctgggcga ggaggtgatg
     1561 gagctgtgcc tgccgatcct gaacggcgcg caggtgcagg gctacgtcat cgccacctac
     1621 acgctggagg gcctgctgac gctgctgccg cccgggctga cgcgcggcca gagcgtggcg
     1681 ctgaccgaac tcgacggcac ccgcctggcg gtgctgggtg caccgcgccg tggcacgcgc
     1741 gtgttcgtgg cgcagcaggt gctggatttg cccggcgcgc cgatggtgct gcggctggag
     1801 ggcgcgcgcc agctgcccga cgtgtttccc aacgttctga cggcactggt caccggcttg
     1861 tcgatcgcgc tggtcacggt gctggcgctg ctggcgggcg acttccggcg ccgccagcgt
     1921 gccgaggccg agctggccga ggcgctcgcg tttcgcaagg cgatggagga ttcgctggtc
     1981 accggcctgc gcgcgcgcga cctggaaggc cgcatcacct acgtcaaccc ggcgttttgc
     2041 gccatggtgg gctatggcgc cgacgagctg atcggccgtg gcgcgcccgc gccctactgg
     2101 ccgcccgagc gcgcgcacga atacggcgag cggctggcgc tgcgctacgg cggccaggcg
     2161 tcggcgcgcg aaggcgttga atcggtgttc atgcaccagg acggcacccg ctttccggtg
     2221 ctgatcttcg aggcgccgct gctcaccagc ggcggccagc aaaccggctg gatgagcgcg
     2281 gtgatggatg tcaccgccca gcgccgggcc gaggaatcat cgcgcgccag ccacgagcgc
     2341 ctgcaggcca gcgcgcgcct ggccacggtg ggcgagatgg cctcgctgat gagccacgag
     2401 ctgaaccagc cgctggccgt gatcgccagc tacgccaacg gctcgctcaa cctgctgcag
     2461 ggcgcgtcgg ccgccgatgc gcacgccacg ctgggcgacg tgcgcaccgc gctggcgcgt
     2521 attgccgagc aggccgggcg cgccggcaag gtgattcaca gcgtgcgcga cctggtgcgc
     2581 cagcgcagca ccgcgcgcga ggcggtgacg ccgcgcgcgc tgttcgacac cgtgctgccg
     2641 ctggtgcaat tgcaggcgcg caagctgggc gccacggtgc agctcgaggt gccgcctgaa
     2701 ttgcccgccg tgtggtgcga gccgaccatg atcgagcagg tgctgctcaa cctggcgcgc
     2761 aacggcctgc aggcgatgga cgaggtggcg ggcgcgcgcg tgctgcggct gcaggcgcgc
     2821 gcgctgccgg tcacggcgct gggctttgtg tcgtcggtcg agttcagcgt ggccgacacc
     2881 ggcggcggca tcagcgaagc ggtggcggcc gaattgttca cgccgttttt caccaccaag
     2941 gccgagggca tgggcttggg cctgtctttg tgccgcacgg tgatcgagca gcacggcagc
     3001 gcgctgcagc accaaccgca gtcgcccagc ggcacggtgt tcagcttcag gctgccgctg
     3061 gcgcgctgaa ttgcttttgt tttgatagct gcttgcgctt gactggcaag cgccggcggc
     3121 ttatttggcc aaatttcgcg gctctggcgg ccacaccatg cgccgcatga tgtgctgcag
     3181 ggcgctggcg ccgtcggtgc agcggccggt gtgcacgggc gaggtctgga tgatggcgct
     3241 gcgccgcgcc gtcagccagt gaaagcgtgc gcgcagcggc aactggccga tcggcccgct
     3301 gcccggggcg ccttcgcaaa tggcgacgac ggcggccagg tgacgctgca ctgccggcag
     3361 atcgacgccg gcgtccagcg cctgcagtcg cgcctcgtcc agcgcgatgg cggcggccag
     3421 aaagccgctg gcctggcacg acaggatcac gccggcgttg acgaactcct cgcgctccac
     3481 gcgcggcacc acgcggatca cggcgtagtc gtacagctca agcgcgcgca cgctcggccc
     3541 cctcaagcca gcgcgcgcgg tcggccacgc gcgcgctgaa gtagtgcagg taggactgcc
     3601 gctgcgccgc cgccgtggcc agcgcgctgc ccggcggctg gtcttgcgcc gacagggcga
     3661 gaaacgattc gggcacctcg tccaccacgc cgcgcagcac atcgggcgtg aggcgtgcgg
     3721 ccagttcggc atcgacctgc gccaacgcgc tggcggcggg cagcagcacg tggtcggcga
     3781 tggcggcaaa cggcttgccc gcgtcggcca ccggcgcctg ccagccgtgg tgaaaggtga
     3841 gcgtggcccc gtggtcgatc agccacggct tgccgtgcca caccagcaag ttggtgttgc
     3901 gcgcgctgcg gtcgacgttg ccgaccagcg cgtcgaacca gacgatgcgc gaggcggtgt
     3961 tggcatccac ctgatcggcg gcggggtcga agttggccga gccgggcagg tagtccagcg
     4021 ccacgttcag gccgccgctg gcgcgcatca ggtcctggat ctcggggtcg ggctcggtgc
     4081 gggcgagttc cacgtccagc tcggccagca cgatctcggg cacgttgagg cccagcgcgc
     4141 gggcgatgcc gccggccaga atttccgcga tcagcgcccg cacgccctgg ccggcgccgc
     4201 gaaacttgag cacgtacaaa ccgtcgtcgt cgccttcgac aatggccggc atcgagccgc
     4261 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcagagga cgcaaaaaaa
     4381 ttcaaagaaa accttttggc tgttctttcg cgtccttcgc gaatcctttg cgtccttcgc
     4441 gtacggtttt cggtcttcga ctgctccttc aattcacccc gtgcgcctgc agcagacact
     4501 ggtgaaacgc ctgtgccgca cgcgacggtt gcggcgagcg gcgcagcacg ctgacgaagt
     4561 cgcatgcata gtgcagcagc gccgaattca ccgcgcgcat ctggccgcgc tgcacgaagg
     4621 cttcggcgta gtgatccggc agaaagccga caaagcggcc agacaggatc agcgtggcaa
     4681 tcgcctcctg ctcgaaggcg gtggccgcgc gcggcaggcg gcggccgtgc gccagcgcca
     4741 tgttgggcga gtggtagccc agcccggcaa agtcctttct ggcccgcagc gctgaccagt
     4801 ccagcgccag gcgctctgaa ttgaagagcg catggtcgtg gccgcaatac agatgcatgg
     4861 tctcgccaaa cagccggtcg tagcgcaggc tggccgaggc gcggtgctcg gggatgacgc
     4921 cgatggcaaa gctgccgtcc atcacgccgc gctcgatggc cgggatgctg cccacgtgca
     4981 ggtgcagctg cacgtcgggc gccgcgcgcg tgaaggcatc gatggcggcg tggatgcgcg
     5041 cggccgggtt gctggccgtc ttgtcgaaca gcgccaccgc cagcgtgccg cccatgcgct
     5101 ggtgtatgtc gtccaccccg gcgcgaaagc cgtccaccgc ggccagcagg cgcagcgtct
     5161 cgtcgtacac gcgctggccc tcggccgtca gcgcaaagcc cgcgcgcccg cgccggcaca
     5221 gcgtgaggcc caggcgcgtt tccagatcct tgatgtgccg gctgacggtg ctggtgccga
     5281 tgttcagctc cagctcggcc gccgacaagc cgccacactc gaccaccgcc ttgaagacct
     5341 gcaacaggcg caggtccatg tcgctgacct ggccgagcac ggcgcgggcg cggggctgga
     5401 gcggatcgtc ttttacttgc atgaatgcgc aaccaaactt gaatatcggt ctatttgaca
     5461 gattaagcac tgctttgaca atgaccgatt cgccgcattc cggttccaga tttcgaggag
     5521 acaagccatg agcgtgaccg ccacccaacc ggccgccgcc cacaccgccc cgcgccagga
     5581 cgccgcctgg ctcgacgcgc actggatgcc cttcaccgcc aaccgccagt tcaagcgcga
     5641 cccgcgcatg atcgtggcgg cgcaaggcgc ctacttcacc gacggcgatg ggcgccaggt
     5701 gttcgacggc ctctcgggcc tgtggtgcac ggggctcggc catggccgac gcgagattgc
     5761 cgaagcggta ggcaagcagg ccgcgcagct cgactattcg cccggcttcc agttcggcca
     5821 tccgctgtcg ttcgagctgg ccaacaagct gaaggccttc acgcccgccg ggctggacta
     5881 cgtcttcttc accgccagcg gctccgaagc cgccgacacc gcgctgaaga tggcccgcgc
     5941 ctactggcgc ctgaagggcc aaggcggcaa gcagctgctg gtggggcgcg agaagggcta
     6001 ccacggcgtt aactttggcg gcatctcggt gggcggcatg gtcggcaacc gcaagctgta
     6061 cggcaccgcc gtgcaagccg atcacctgcc gcacacgcag ccgcccatgg gcacctttgc
     6121 caagggccaa ccggccgaag gcggcaagca gctcgccgac cgcctgctgg acctgatcgc
     6181 gctgcacgac gccagcaaca tcgccgccgt catcgtcgag cccttcagcg gctcggccgg
     6241 cgtggtgatt ccgcccgtgg gctacctgca gcgcctgcgc gaaatctgca cccagcacga
     6301 catcctgctg atcttcgacg aggtcatcac cggctttggc cgctgcggca gcaggacggg
     6361 ggccgatgcg tttggcgtgg tgcccgacat catgaccttc gccaagcaag tcaccaacgg
     6421 cgtgcagccg ctgggcggcg tgatcgcatc aaaagcgatt tacgacacct tcatggccgc
     6481 cggcgggccg gactacatgc tggaatttgc ccacggctac acctacagcg cgcaccccgt
     6541 cgcctgcgcc gccggcatcg ccgcgatgga tttgctggag cgcgaagacg gcgtgggtcg
     6601 cgtgaaagca ctggcgccgc acttcgaggt tgccgtgcac agcctgaaag gggccaagca
     6661 cgtgctcgac atccgcaact acggactggc tgccggcctc accatcgccc ccgcacccgg
     6721 tgagcccgcc aagcgcccct acgaaatcg
//
LOCUS       DAOMVO010000119         6725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3054_length_6725_cov_16.313643, whole genome shotgun sequence.
ACCESSION   DAOMVO010000119 DAOMVO010000000
VERSION     DAOMVO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3054_length_6725_cov_16.313643"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKV30_07210"
     CDS             complement(<1..683)
                     /locus_tag="PKV30_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRL30038.1"
                     /translation="MFKLTSLNLNGIRSATTKGVEAWLERHAPDCICVQEMKAQHADI
                     EGRFEVLAGMRGHFHLATAKKGYSGVGIYTRHEPSEVIAGYGSPEFDEEGRYLEARFD
                     TPQRKFSVISCYFPSGSSGPERQEAKYRFLAEFYPVLHRLKAERDFVLCGDVNVAHQQ
                     IDLKNWKSNQKNSGFLPEERAWVTRVIDELQLVDVYRRLHPETTDEAYTWWSNRGQAY
                     AKNVGWRLD"
     gene            699..1367
                     /gene="pyrE"
                     /locus_tag="PKV30_07215"
     CDS             699..1367
                     /gene="pyrE"
                     /locus_tag="PKV30_07215"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRL30039.1"
                     /translation="MTTDRDALAGEFVQFAIDAGVLRFGDFKTKAGRQSPYFFNAGLF
                     DDGAKLARLAQFYARAIAQSGLEFDVIFGPAYKGIPLGAGVAMELARLGLVKPFAYNR
                     KEAKDHGEGGTLVGAPLRGRVLIVDDVISAGTAVRESIALIKAAGATPHAVAIALDRQ
                     EKATENGADVPWSAVQYVRDKLGLQVVSIANLAQLLQHLGQSDNFLPHFEAVSAYRAR
                     YGVD"
     gene            1373..2074
                     /locus_tag="PKV30_07220"
     CDS             1373..2074
                     /locus_tag="PKV30_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRL30040.1"
                     /translation="MNRLPPLRALAPAARALRWLAAACVLVAASGAALAQTTTTTSIY
                     TCVDAQGRRLTSDRPILACNDREQRELNSSGTTRRVIQPTMTASEREAREARERDAAV
                     ERQRAQNIIRRDQALITRYPDKAAHEAGRKEAMAQTQTVVDAANARIAELAAERKALD
                     DEMEFYKKDPSKAPPKVRRGIEDNAQAVEAQQRAIAGQQEERNRINARFDEEAARLKP
                     LWQAKAADAAAPAKR"
     gene            complement(2142..3614)
                     /gene="gatB"
                     /locus_tag="PKV30_07225"
     CDS             complement(2142..3614)
                     /gene="gatB"
                     /locus_tag="PKV30_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRL30041.1"
                     /translation="MSAKLIQGYEVVIGFETHAQLQTNSKIFSRASTAFGAAPNTQAC
                     AVDLALPGALPVMNREAVHCAIKLGLALGSHIAPRSIFARKNYFYPDLPKGYQISQFE
                     IPVVQGGEVAFFVPSTSSGQAALEKKIVRLVRAHLEEDAGKSLHEDYHGMSGIDLNRA
                     GTPLLEIVTEPDMRSSAEAVAYAKELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGD
                     KLGTRREIKNLNSFKFMQQAIDFEIRWQIEQIEDGHAIQQATVLFDPDTGETRAMRSK
                     EDSADYRYFPDPDLPPLCISEQWIEETRGQMPELPRKMAERFVADYGLPEYDATTLTQ
                     SPAMAAYFEQAAQTSGQPKLASNWVMGEISKRLNAEDIGIDAAKVSAAQLAQLIGRIQ
                     DGTISNAAARQVFEALWSGEGAEVDAVIAAKGLKQMNDSGALEKIIDEVIAANPANVE
                     QFKAGKDKAFNALVGQVMKASQGKANPAQVNELLRARLAG"
     gene            complement(3616..5079)
                     /gene="gatA"
                     /locus_tag="PKV30_07230"
     CDS             complement(3616..5079)
                     /gene="gatA"
                     /locus_tag="PKV30_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRL30042.1"
                     /translation="MSALHDLTVAQLAAKLKAREVSAVEAAQHFLARSRQHADLGAYL
                     AQDEAVTLAQAQAADARLAAGDAPLLAGVPIAHKDIFVTTDFPTTAGSKMLAGYRSPF
                     ESTVTSLFQRQGMVCLGKLNCDEFAMGSANENSAYGPVKNPWDVSRVPGGSSGGSAAA
                     VAARLTPAATGTDTGGSIRQPASFCGLTGIKPTYGRASRYGMIAFASSLDQAGPMART
                     AEDCALLLSAMCGPDPDRDSTSLDVPAEDFTRALNGDLKGLRIGVPKEFFGDGLAPDV
                     RAAGEAALAELQKLGATRVDISLPRTELSIPAYYIIAPAEASSNLSRFDGVRYGHRAR
                     HYTDLEDMYKKTRAEGFGDEVKRRIMTGTYVLSHGYYDAYYLQAQKIRRMIADDFQRA
                     FQQCDVIAGPVAPSVARRIGECADDPLADYLADVYTLPASLAGLPGMSVPAGFGEGGM
                     PVGLQLIGNYLQESKLLNVAHQLQRATDFHLRKPEGY"
     gene            complement(5082..5381)
                     /gene="gatC"
                     /locus_tag="PKV30_07235"
     CDS             complement(5082..5381)
                     /gene="gatC"
                     /locus_tag="PKV30_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRL30043.1"
                     /translation="MALTDQDIGRIAHLARLELSADESKRMLTRLNGFFGTVEAMCAV
                     DTQGVEPLTQPIAAVQDVHLRLRDDVASEPDDRAANQRSAPAVEDGLYLVPKVIE"
     gene            5584..6627
                     /locus_tag="PKV30_07240"
     CDS             5584..6627
                     /locus_tag="PKV30_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRL30044.1"
                     /translation="MFGAFRRYFSTDLAIDLGTANTLIYVRDKGIVLDEPSVVAIRHE
                     GGPQGKKTIQAVGAEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHPR
                     GLFKPSPRIIICVPCGSTQVERRAIRESALGAGASEVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSARVGGDKFDDAIINYIRRNYGMLIGDPTAEM
                     IKKEIGSAFPGSEVKEMEVKGRNLSEGVPRSFTISSNEILEALTEPLNSIVSEVKKAL
                     EETPPELGADIAERGMMLTGGGALLRDLERLLAEETGLPVLVAEQPLTCVVRGCGLAL
                     ERMERLGNIFTNE"
BASE COUNT         1125 a         2168 c         2268 g         1164 t
ORIGIN      
        1 tagtccagcc gccagccgac gtttttggcg taggcctggc cgcggttgct ccaccaggtg
       61 taggcctcgt cggtggtctc ggggtgcagg cggcggtaga cgtcgaccag ttgcagctcg
      121 tcgatcacgc gggtgaccca ggcgcgctct tcgggtagga agcctgagtt tttctggttg
      181 ctcttccagt tcttcaaatc gatttgctgg tgcgccacgt tgacgtcgcc gcacagcaca
      241 aagtcgcgct cggccttcag gcggtgcagc acggggtaga actcggccag aaagcggtat
      301 ttggcctctt gccgctcggg gccggacgag ccgctgggga agtagcagct gatgaccgag
      361 aactttcgct gcggcgtgtc gaagcgcgcc tcgaggtagc gcccctcctc gtcgaactcg
      421 ggcgagccgt agccggcgat cacctcgctc ggctcgtggc gcgtgtagat gccgacgccc
      481 gaatagccct ttttggcggt ggccaggtga aagtgcccgc gcatgccggc cagcacctcg
      541 aaacggcctt cgatgtcggc gtgctgcgcc ttcatctcct gcacgcaaat acaatccggc
      601 gcgtgccgct ccagccaggc ttcgacgccc ttggtggtgg ccgagcgaat gccattcagg
      661 ttcaggctgg tgagtttgaa cacaagggga tttcctgtat gaccacggac cgcgatgcgt
      721 tggcgggtga attcgtgcag ttcgccatcg acgccggcgt gctgcgcttt ggcgatttca
      781 agaccaaggc gggccggcaa agcccctact ttttcaacgc cgggctgttt gacgacggcg
      841 ccaagctggc gcgcctcgcc caattctacg cacgggccat cgcgcagtcg gggctggaat
      901 tcgacgtcat cttcggcccc gcgtacaagg gcatccccct gggtgcgggg gtcgcgatgg
      961 agctggctcg gctaggatta gtaaaaccat ttgcctacaa ccgaaaggaa gccaaggacc
     1021 atggcgaagg cggcacgctg gtcggcgcgc cgttgcgtgg tcgggtgctg atcgtggacg
     1081 acgtgatttc tgccggcacc gcggtgcgcg agtcgattgc gctgatcaag gcggccggcg
     1141 ctacgccgca cgcggtggcc atcgcgctcg accggcagga gaaggcgacc gagaatggcg
     1201 ccgacgtccc ttggtcggcc gtgcaatatg tccgcgacaa gctggggttg caggtggttt
     1261 ccatcgccaa tctggcgcag ttattgcagc acctgggaca gagtgacaat tttttgccgc
     1321 atttcgaagc cgtgtcagcc taccgggccc gctacggcgt tgattgactg acatgaaccg
     1381 cttgccgcct cttcgtgccc ttgcccctgc cgcgcgcgcc ctgcgctggc tggcggccgc
     1441 gtgcgtgctg gtggcggcca gcggcgcggc gctggcgcaa accaccacca ccaccagcat
     1501 ctacacctgc gtcgatgccc agggccggcg cctgacgtcc gaccggccga ttctggcctg
     1561 caacgaccgc gagcagcgcg aactcaacag cagcggcacc acgcgccgcg tgatccagcc
     1621 gaccatgacg gccagcgagc gcgaggcgcg tgaagcgcgc gagcgcgacg ccgcggtgga
     1681 gcgccagcgc gcgcaaaaca tcatccggcg cgaccaggcg ctgatcacgc gctaccccga
     1741 caaggccgcg cacgaggccg gccgcaagga agccatggcg cagacgcaaa ccgtggtcga
     1801 cgccgccaat gcccgcatcg ccgaactggc ggccgaacgc aaggcgctgg acgacgagat
     1861 ggagttctac aagaaagacc cgtcgaaggc gccgcccaag gtgcgccgcg gcatcgagga
     1921 caacgcgcag gccgtcgagg cgcaacagcg cgccatcgcc ggccagcagg aagagcgcaa
     1981 ccgcatcaac gcccgcttcg acgaggaagc cgcgcgcctg aagccgctgt ggcaggccaa
     2041 ggcggcggat gccgcagccc ccgccaagcg ctgatttttt gaatcaaatc agccgccagc
     2101 gcttgtccag caagcgctgg cagctctcat aaacagagca atcaacccgc cagccgggcg
     2161 cgcaacaact cgttcacctg cgccggattg gccttgccct ggctggcttt catcacctgg
     2221 ccgaccagcg cgttgaaggc cttgtccttg ccggccttga actgctcgac gttggccgga
     2281 ttggcggcga tgacctcgtc gatgatcttc tccagcgcgc cggagtcgtt catttgcttc
     2341 aagcccttgg cggcgatgac ggcgtcgacc tcggcgccct cgcccgacca cagcgcctcg
     2401 aacacctgcc gcgcggcggc gttgctgatg gtgccgtcct ggatgcggcc gatgagctgc
     2461 gccagttgcg cggccgacac cttggcggcg tcgatgccga tgtcctcggc gttcagccgc
     2521 ttggagattt cgcccatcac ccagttgctg gccagcttgg gctggccgct ggtctgggcc
     2581 gcttgctcga agtacgcggc catcgcgggc gattgcgtca gcgtcgtcgc gtcgtactcg
     2641 ggcagaccgt agtccgccac aaagcgctcg gccatttttc gcggcaactc gggcatttgt
     2701 ccacgtgttt cctcgatcca ttgctctgaa atgcagagcg gtggcaagtc gggatcgggg
     2761 aagtagcggt agtcggccga atcttccttg ctgcgcatgg cacgggtttc gccggtgtcg
     2821 gggtcgaaca gcacggtggc ctgttgaatg gcgtggccgt cctcgatctg ctcgatctgc
     2881 cagcgaatct cgaagtcgat cgcctgctgc atgaacttga agctgttcag gttcttgatc
     2941 tcgcgccgcg tgccgagctt gtcgccgggc ttgcgcacgc tgacgttggc gtcgcagcga
     3001 aagctgcctt cctgcatgtt gccgtcgcaa atgccgatcc aggtgacgat cttgtgcagt
     3061 tccttggcat aagccacggc ctcggcggac gagcgcatgt cgggctcggt cacgatttcc
     3121 aacagcggcg tgccggcgcg gttcaggtcg atcccgctca tgccgtggta gtcctcgtgc
     3181 agcgacttgc cggcgtcttc ttcgaggtgc gcgcgcacca ggcggacgat ttttttctcg
     3241 agagccgcct gtcctgagct tgtcgaaggg acaaagaacg ccacttcgcc gccctgcacc
     3301 accggaatct cgaactggct gatctggtag cccttgggca gatcggggta gaagtagttc
     3361 ttgcgggcaa aaatgctgcg cggcgcaatg tgggagccaa gggccaggcc caatttgata
     3421 gcgcagtgca ccgcctcgcg gttcatcacc ggcagcgcgc cgggcagcgc cagatccacc
     3481 gcgcaggcct gcgtgttggg cgcggcgcca aaagcggtgc tggcgcggct gaagatcttg
     3541 ctgttcgttt gcaactgggc gtgcgtctcg aagccgatga cgacttcgta gccctggatc
     3601 aacttggctg acatatcaat agccctccgg cttgcggagg tggaagtcgg tcgcccgctg
     3661 cagctggtgc gccacgttca gcaatttcga ctcttgcagg taattgccga tgagctgcag
     3721 gcccaccggc atgccgcctt cgccaaagcc ggccggcacg ctcatgccgg gcaggccggc
     3781 aagcgaggcg ggcagcgtgt agacgtcggc caggtaatcg gccagcgggt cgtcggcgca
     3841 ttcgccgatg cggcgcgcca cgctgggcgc aaccgggccg gcgatgacgt cgcactgctg
     3901 gaaggcgcgc tgaaaatcgt cggcgatcat gcggcgaatc ttctgcgcct gcaggtagta
     3961 ggcgtcgtag tagccgtgcg agagcacgta ggtgccggtc atgatgcggc gcttgacctc
     4021 gtcgccaaag ccttcggcac gcgttttctt gtacatgtcc tccaggtcgg tgtagtgccg
     4081 ggcgcggtgg ccgtagcgca cgccgtcgaa gcgcgacagg ttggagctgg cttcggccgg
     4141 cgcgatgatg tagtaggcgg ggatggacag ctcggtgcgc ggcagcgaga tgtcgacccg
     4201 cgtggcgccg agcttttgca gctcggccag cgccgcttcg ccggcggcgc gcacgtcggg
     4261 cgccaggccg tcgccgaaaa actccttcgg cacgccgatg cgcaggcctt tcagatcgcc
     4321 attcagcgcg cgggtgaagt cttctgccgg cacgtcgagc gaggtggaat cgcggtccgg
     4381 gtcgggcccg cacatggccg acagcagcag tgcgcagtct tcggcggtgc gcgccatcgg
     4441 gccggcctgg tcgaggctgg aggcaaaggc aatcatgccg taacggctgg cgcggccgta
     4501 ggtcggcttg atgccggtca ggccgcaaaa actggccggt tggcggatcg agccgccggt
     4561 gtcggtgccg gtggcggcgg gcgtcaggcg cgcggccacc gccgcggcgc tgccgccgga
     4621 cgagccgccg ggcacgcgcg acacatccca cgggttcttc accgggccgt aggccgagtt
     4681 ctcgttggcc gagcccatgg cgaactcgtc gcagttcagc tttccaagac aaaccatgcc
     4741 ctggcgctgg aacaggctgg tcaccgtgct ctcaaaagga gagcgatagc cggccagcat
     4801 cttcgagccg gcggtggtcg ggaaatcggt ggtgacgaag atgtccttgt gcgcgatggg
     4861 cactccggcc agcagcggcg cgtcgcccgc ggccaggcgc gcatcggccg cctgggcctg
     4921 cgccagcgtc acggcttcgt cttgcgccag gtaggcgccc aggtcggcgt gctggcggct
     4981 gcgcgccaga aagtgctgcg cggcttcaac cgccgacacc tcgcgcgcct tcagcttggc
     5041 ggccaactgg gccaccgtca ggtcatgcaa ggcgctcatg cttattcgat cactttcgga
     5101 accagataca gcccatcttc caccgccggc gcgctgcgct ggttggcggc gcggtcgtcc
     5161 ggctcgctgg ccacgtcatc gcgcaggcgc aagtgcacgt cctgcacggc agcgatgggt
     5221 tgcgtgagcg gctcgacgcc ctgcgtgtcg acggcgcaca tggcctcgac ggtgccgaag
     5281 aagccgttca gacgggtgag catccgctta ctttcgtccg cactcaactc aagccgcgcc
     5341 aaatgggcga tgcggccgat gtcctgatcg gttaaagcca tgaaaaacgc actggaaacc
     5401 agatgtaatt tgaacttttg gcctcaggcg ccaaaccagc gcgcaagggg gatgcgttat
     5461 tatcctgcgt ttagtgcaac ggcccggaaa cgggccgtat ttgcacgaat tcacgcctaa
     5521 ttcgcatcga atcagggggt tacggcgtct ggtgcttgcc ggcaacccca caaaggacac
     5581 tgcatgtttg gagctttccg tcggtatttc tcgaccgacc tggccattga cctcggcacc
     5641 gccaacaccc tgatctacgt gcgcgacaag ggcatcgtgc tggacgaacc gtcggtcgtc
     5701 gccatccggc acgaaggcgg cccgcagggc aagaagacga ttcaggccgt gggcgccgaa
     5761 gccaaggcca tgctgggcaa ggtgccgggc aacatcgagg cgatccgccc gatgaaggac
     5821 ggcgtgatcg ccgacttcac cgtcaccgag cagatgctca agcagttcat ccgcatggtg
     5881 cacccgcgcg gcctgttcaa gcccagcccg cgcatcatca tctgcgtgcc ttgcggctcg
     5941 acgcaggtcg agcgccgcgc gatccgcgaa tcggcgctgg gcgcgggcgc aagtgaggtg
     6001 tacctgatcg aggagccgat ggccgccgcc atcggcgccg gcctgccggt gtctgaggcc
     6061 agcggctcga tggtggtcga catcggcggc ggcacgaccg aggtgggcgt gatctcgctc
     6121 ggcggcatgg tctacaaggg cagcgcccgc gtgggcggcg acaagttcga cgacgccatc
     6181 atcaactaca tccgccgcaa ctacggcatg ctgatcggcg acccgacggc ggagatgatc
     6241 aagaaagaga tcggcagcgc cttccccggc agcgaagtca aggagatgga ggtcaagggc
     6301 cgcaacctct ccgaaggcgt gccgcgcagc ttcaccatct caagcaacga gatcctggaa
     6361 gcgctgaccg agccgctcaa cagcatcgtg tctgaagtga agaaggcgct ggaagaaacc
     6421 ccgcccgagc tcggcgccga cattgccgag cgcggcatga tgctgaccgg cggcggcgcc
     6481 ctgctgcgcg acctggagcg cctgctggcc gaagaaaccg gcctgcccgt gctggtggcc
     6541 gagcagccgc tgacctgcgt ggtgcgtggc tgcgggctgg cgctggagcg gatggagcgc
     6601 ctgggcaaca ttttcacgaa tgaataggac gccccctgtg gcgctgcgcg ccttccccca
     6661 ctgggggaca acgctggcgg ccggggaaac cccggccacg gcgttcccgg gtggcctgct
     6721 ccgcg
//
LOCUS       DAOMVO010000120         6632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3105_length_6632_cov_12.350616, whole genome shotgun sequence.
ACCESSION   DAOMVO010000120 DAOMVO010000000
VERSION     DAOMVO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_6632_cov_12.350616"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /gene="xseA"
                     /locus_tag="PKV30_07245"
     CDS             complement(<1..994)
                     /gene="xseA"
                     /locus_tag="PKV30_07245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRL30045.1"
                     /translation="MNTGFEPLPPATAPRVWPVGALTRAVASALEARFNPVAVRGEVL
                     GFTRAGSGHCYFALKDEAGQLRCAMFRRAAGLLGFNPREGDQVEVRGRLTVYEPRGDL
                     QLVVESMQRAGQGALFEQFLRLKERLEAEGLFDAAAKRPLPAWPRGIGLVTSRGAAAL
                     HDVLTALARRAPHVPVLLAPAAVQGAAAPAELRAALQKLYLLAQAGKAPAADLALNST
                     PPIDVILLVRGGGSIEDLWAFNDEALARTIAASPVPVICGVGHETDFSIADFVADLRA
                     PTPTAAAELVSAPRDQALRELQAQHERLQRALRRRLDGEAQRLDGLHGRLARPAD"
     gene            1376..2029
                     /locus_tag="PKV30_07250"
     CDS             1376..2029
                     /locus_tag="PKV30_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRL30046.1"
                     /translation="MLSIIQAAGWPIWPLILCSILALAFIVERFISLKNSRVLGSGLV
                     DEAIGISRTAVPAPETVNQLAQHSALGEVLAAGWRAINNNPRCSPDEMRAAMEAAGRT
                     VTHRLERYLPALATIASAAPLLGLLGTVIGMIEIFGSQAPSGALSGGNPAQLAHGISV
                     ALYNTAFGLIVAIPALIFWRYFRARVDGYTLELELSAERFARHLEPLCPGRSAEAKR"
     gene            2026..2439
                     /locus_tag="PKV30_07255"
     CDS             2026..2439
                     /locus_tag="PKV30_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRL30047.1"
                     /translation="MKFRPHPPEEPEINLIPFIDVLLVILIFLMLTTTYSKFTELQVT
                     LPTASADAARDRPKEIVVAVAADGRYAVNKQPLAGNSVEVVANALRAVAAKDSVLIIS
                     ADAAAPVQAAVTAMDAARRVGLTQITFAAKTGASP"
     gene            2633..3700
                     /gene="lpxK"
                     /locus_tag="PKV30_07260"
     CDS             2633..3700
                     /gene="lpxK"
                     /locus_tag="PKV30_07260"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRL30048.1"
                     /translation="MLRAFIEHRLRAAWQRRGPLAVLLWPLSRLYGALAARQRAVDAS
                     KAVRLSVPVVVVGNVIAGGAGKTPTTLAIVQHLKARGWRPGIVSRGHGRASSDCREVG
                     PGADPQDVGDEPLLLRRRADVPVFVAPRRAEAGRALLAAHPACDILVCDDGLQHVALA
                     RDVEICVFDARGIGNGWLLPAGPLREPWPRAVDLVLYTEAPPKPVGAGLPAMAAPAEH
                     EFPIAGKPAPTTFTATRHLADHALRTDGTRVPLAELRGQPLTAVAGIARPEAFFTMLR
                     AAGLTLADTIALPDHYDFNSWSRLPDKRQGLICTEKDALKLWRHEPNALAVPLVLDVP
                     PAFFSALDERLAAQGYHPRSP"
     gene            3697..>4031
                     /locus_tag="PKV30_07265"
     CDS             3697..>4031
                     /locus_tag="PKV30_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL30049.1"
                     /translation="MISHIDHVVLTVADIERSVAFYQRVLRMEAVTFGSGRRALKFGQ
                     QKINLQTLGQELRNRAGVGSGDLCLITEWALPDVMAHLAAEGVPLLEGPVTRSGAQGP
                     IESLYFNDP"
     assembly_gap    4032..4131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4227..4436
                     /locus_tag="PKV30_07270"
     CDS             4227..4436
                     /locus_tag="PKV30_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRL30050.1"
                     /translation="MDPKLLELLVCPVTKGPLDFDRDKAELISRSARLAYPVRDGIPV
                     MLEGEARTLSDEELEALPPRTPLAG"
     gene            4436..5197
                     /gene="kdsB"
                     /locus_tag="PKV30_07275"
     CDS             4436..5197
                     /gene="kdsB"
                     /locus_tag="PKV30_07275"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRL30051.1"
                     /translation="MFTVLIPARLASTRLPNKPLADIGGKPMVVRVAEQAEKSGAAQV
                     VVAADAPEILAACSAHGVRAVPTRADHPSGSDRLAEACALLGLMGDDVVVNVQGDEPL
                     IEPALIQAVADLLSKEPLAHMGTAAHAIKSVADFVNPNVVKVVTDARGLAMTFSRAPL
                     PWWRDGFAEGITQLPTQPAPLRHIGIYSYRAGFLRAFPTLSPAPLEQCEALEQLRALW
                     HGHRIAVHVAPHAPGPGVDTPEDLARVQALFAQAA"
     gene            5328..5984
                     /gene="adk"
                     /locus_tag="PKV30_07280"
     CDS             5328..5984
                     /gene="adk"
                     /locus_tag="PKV30_07280"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRL30052.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQQYGIPQISTGDMLRAAVKAGTP
                     LGLQAKSVMESGGLVSDDLIINLVKERIAQPDCANGFLFDGFPRTIPQAEAMKAAGVK
                     LDYVLEIDVPFDAIIERMSGRRSHPASGRTYHVKFNPPKAEGVDDVTGEPLVQRDDDK
                     EDTVKKRLDVYTQQTRPLVDYYSQWAAKEPAAAPKYRKISGTGSVDDITARALQALAS
                     "
     gene            complement(6044..6286)
                     /locus_tag="PKV30_07285"
                     /pseudo
     CDS             complement(6044..6286)
                     /locus_tag="PKV30_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
     gene            6263..6604
                     /locus_tag="PKV30_07290"
     CDS             6263..6604
                     /locus_tag="PKV30_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30053.1"
                     /translation="MHAGGAQGAFRIAAEEAQARWDKACNEFLKNALVVVVMAGFLAI
                     KFWTPATPAQPVQATPPPAAAQAAKPTAPPAAAKTAKKPPPKPSKPRTVEQCLKIADE
                     HVMVRCLERAP"
BASE COUNT          994 a         2340 c         2207 g          991 t
ORIGIN      
        1 cgtcggccgg gcgcgccagg cggccgtgca gcccgtcgag ccgctgcgcc tcgccgtcca
       61 ggcggcggcg cagggcgcgt tgcaggcgct cgtgctgggc ctgcagctcg cgcagggcct
      121 gatcgcgcgg tgcgctgacc agctcggcag cggcggtcgg cgtgggtgcg cgcaggtcgg
      181 cgacgaaatc ggcgatgctg aaatcggtct cgtgccccac cccgcagatc accggcaccg
      241 ggctggcggc gatggtgcgg gccaaggcct cgtcgttgaa ggcccacaaa tcctcgatcg
      301 agccgccgcc gcgcaccagc aggatgacgt cgatgggcgg cgtgctgttc aatgccaaat
      361 cggctgctgg cgctttacca gcctgcgcga gcagatacaa cttttgtagc gcggcgcgca
      421 gctcggccgg cgccgccgca ccttgtaccg ccgccggcgc cagcagcacc ggcacgtgcg
      481 gcgcgcgccg tgccagcgcc gtcagcacgt cgtgcagcgc cgctgcgccg cgcgaagtga
      541 ccaggccgat accgcgcggc caggccggca gcgggcgctt ggcggcagcg tcgaacaggc
      601 cttcggcctc gagccgctct ttcagccgca gaaactgctc gaacagcgcg ccctgccccg
      661 cgcgctgcat cgattcgacc accagttgca ggtcgccgcg cggctcgtac accgtcaggc
      721 gcccgcgcac ctcgacctga tcgccctcgc gcgggttgaa gcccagcagc ccggcggcgc
      781 ggcggaacat ggcgcagcgc agctggccgg cctcgtcctt cagcgcgaag tagcaatggc
      841 cgctgccggc gcgcgtgaag cccaagactt cgccgcgcac cgccacgggg ttgaagcgcg
      901 cctccagtgc gctcgccacg gcgcgggtca gcgcgccaac tggccacaca cggggcgccg
      961 ttgctggggg cagcggctca aatccagtgt tcatgcggtc tggcgaacca ttttgcggcg
     1021 gcgggcggcg ccagtactcc acaacgggcg gcgagcgggg ccggcgctgc atgcatgcgc
     1081 cgtaaagcac agtgccgtgg tgcaagtcct tgtttttaaa cggttttcag ctgccttttg
     1141 cttcatcaat ctgtcacatc cggcgccagg actgggctgc gggcgcggcg caggcgtttt
     1201 cttcacaaag ttatccacag aatttgtgcg ttcccgcggt ggctgacaaa tggggtgctg
     1261 gtgtggtctt gttggcggcg aaggcggcgg cgggggcgct ggcggccggg gcgcatggca
     1321 cgctgcgcca taatcgcgcg catctttgca tggccacgtg gcccgaaaga agcccttgct
     1381 ttccatcata caagccgctg gctggccgat ctggccgctc atcctgtgtt ccatcctggc
     1441 cttggcgttc atcgtcgagc gcttcatcag cctcaaaaac agccgcgtgc tgggcagcgg
     1501 gctggtggac gaggcgattg gcatctcgcg caccgcggtg cccgcgcctg aaaccgtgaa
     1561 ccagctggcg cagcattcgg cgctgggcga ggtgctggcc gccggctggc gcgccatcaa
     1621 caacaacccg cgctgcagcc cggacgagat gcgcgccgcc atggaagcgg ccggccgcac
     1681 cgtcacgcac cggctggagc gctatttgcc cgcgctggcc accatcgcct cggcggcgcc
     1741 gctcctgggc ctgctgggca cggtgatcgg catgatcgag atttttggct cgcaggcgcc
     1801 gtcgggcgcg ctgtcgggcg gcaacccggc gcaactggcg cacggcattt cagtggcgct
     1861 gtacaacacg gcgttcggcc tgatcgtggc gatcccggcg ctgatcttct ggcgctactt
     1921 ccgcgcccgg gtcgacggct acacgctgga gctggagctg tccgccgagc gctttgcgcg
     1981 ccacctggag ccgctgtgcc ccggccgcag cgcggaggcc aagcggtgaa gttccgcccg
     2041 cacccgccgg aggagccgga gatcaacctg atcccgttca tcgacgtgct gctggtgatc
     2101 ctgatcttct tgatgctgac gaccacgtac agcaagttca ccgagctgca ggtcaccctg
     2161 cccaccgcca gcgccgacgc cgcgcgcgac cggccgaagg aaatcgtcgt ggccgtggcc
     2221 gccgacgggc gctatgccgt caacaagcag ccgctcgccg gcaacagcgt cgaggtggtg
     2281 gcgaatgcgc tgcgcgccgt ggcggccaag gacagcgtgc tcatcatcag cgccgacgcc
     2341 gccgcgccgg tgcaggccgc cgtgaccgcg atggacgcgg cacggcgcgt ggggctgacg
     2401 cagattacgt ttgcagcgaa gacgggggca tccccctgag cggctgacgc cgcttcgccc
     2461 ttctttcttc gggaaggggg acgcacccag cgggcgggcc aagcccttcc gcgggtgccc
     2521 gcgcatggcc tgctccgcgg cgttctgacc cgtgccagac gcggtgacga actggctatg
     2581 ctgcgcatgg actgatcggc gccatccgca gccgcatttc gcctcaccaa ccatgcttcg
     2641 cgccttcatc gaacaccgcc tgcgcgccgc ctggcagcgg cgcgggccgc tggccgtgct
     2701 gctttggccg ctgtcgcgcc tgtacggcgc gctggcggcg cggcagcggg cggtggatgc
     2761 gtccaaggcc gtgcgcctgt cggtgccggt cgtggtcgtg ggcaacgtca tcgccggcgg
     2821 cgcgggcaag acgccgacca cgctggccat cgtgcagcac ctcaaggcgc gtggctggcg
     2881 gccgggcatc gtgtcgcgcg ggcacgggcg tgcgagcagc gactgccgcg aagtcggccc
     2941 cggcgccgat ccgcaagacg ttggcgatga gccgctgctg ctgcgccggc gtgccgacgt
     3001 gccggtgttt gtcgccccgc gccgcgccga ggccggccgc gcgctgctgg ccgcgcaccc
     3061 ggcgtgcgac atcctggtct gcgacgatgg cctgcagcac gtggcgctgg cgcgcgacgt
     3121 ggaaatttgc gtattcgacg cgcgcggcat cggcaacggc tggctgctgc ccgccggccc
     3181 gctgcgcgag ccgtggccgc gcgcggtgga tttggtgctc tacaccgagg cgccgcctaa
     3241 acctgtggga gcgggcttgc ccgcgatggc agcgccggcc gaacacgaat ttcctatcgc
     3301 aggcaagccc gctcccacga ccttcaccgc cacgcgccat ctcgccgatc acgccctgcg
     3361 caccgacggc acgcgcgtgc cgctggccga actgcgcggc cagccgctca ccgccgtggc
     3421 cggcatcgca cggcccgaag ccttcttcac catgctgcgc gccgccgggc tgacgctggc
     3481 agacaccatc gccttgcccg atcactacga tttcaatagc tggtcgcgct tgccagacaa
     3541 gcgccagggc ctgatttgca ctgaaaaaga cgccctcaag ctgtggcgcc acgaacccaa
     3601 cgcgctggcc gtgccgctgg tgctcgacgt gccgccagcc ttcttcagcg cgctggacga
     3661 gcgcctggcc gcgcagggct atcatccgcg cagcccatga tttcgcacat cgaccacgtc
     3721 gtcctcacgg ttgccgacat cgagcgctcc gtcgccttct accagcgcgt gctgcgcatg
     3781 gaagcggtca cctttggcag cggccggcgc gccctgaagt tcggccagca gaagatcaac
     3841 ctgcagacgc tggggcaaga gttgcgcaac cgcgccggcg tcggctcggg cgatctgtgc
     3901 ctgatcaccg agtgggcgct gccggacgtg atggcccacc tggcggccga gggcgtgccg
     3961 ttgctcgagg gcccggtgac ccgttccggc gcgcaggggc cgatcgaatc gctgtatttc
     4021 aacgacccgg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccagcgtct
     4141 ggatgccccg gcgctgatcg gcatggccct catcgtcgtc ggcgtgctgt tgatgaacct
     4201 ttcttccacc tccgcaggat attgagatgg atcccaagct gcttgaactg ctggtctgcc
     4261 ccgtcaccaa gggcccgctt gacttcgacc gcgacaaggc cgaactgatc tcgcgctcgg
     4321 cccgcttggc ctacccggtg cgcgacggca tcccggtgat gctggaaggc gaggcgcgca
     4381 cgttgtcgga cgaggaactc gaagccctgc ccccgcgcac gccgctggca ggctgatgtt
     4441 caccgtcctc atcccggcgc gcctggcttc cacccgcctg ccgaacaagc cgctggccga
     4501 catcggcggc aagccgatgg tggtgcgggt ggccgagcag gccgaaaagt cgggcgccgc
     4561 gcaggtggtg gtggcggccg acgcgcccga gattctggcc gcctgcagcg cccacggcgt
     4621 gcgcgccgtg cccacccgcg ccgaccaccc gagcggcagc gaccgcctgg ccgaagcctg
     4681 cgcgctgctg ggcctgatgg gcgacgacgt ggtggtcaac gtgcagggcg acgaaccgct
     4741 gatcgagccg gcgctgatcc aggccgtcgc cgatttgttg tcaaaagagc cgctggcgca
     4801 tatggggact gcggcgcacg ctatcaaaag cgttgcagac ttcgtcaacc ccaacgtcgt
     4861 caaggtggtc accgacgcgc gcggcctggc gatgactttc agccgcgcgc ccctgccgtg
     4921 gtggcgcgac ggctttgccg agggcatcac gcaactgccc acgcaaccgg cgccgctgcg
     4981 ccacatcggc atctacagct accgcgccgg ctttctgcgc gcctttccca cgctcagccc
     5041 cgcgccgctg gagcagtgcg aagcgctgga gcagctgcgc gcgctttggc atggccaccg
     5101 catcgccgtg cacgtggcgc cgcacgcgcc cgggcccggc gtcgacacgc ccgaggatct
     5161 ggcccgcgtg caggcgctgt tcgcgcaggc cgcctgacgc gggtttcggg gcggctccaa
     5221 cgcgcccggt tgtcagccgc gcagagggtc gcgcatgcta ttcttgattg caacgacaag
     5281 gccgcgccgc atgcagcgtg ccccgacgca ttcgattgag gagcaagatg agactgattc
     5341 ttctgggcgc ccccggcgcc ggcaagggca cgcaagcggc cttcatctgc cagcaatacg
     5401 gcatcccgca aatctccacc ggcgacatgc tgcgcgccgc cgtcaaggcc ggcacgccgc
     5461 tcggcctgca ggccaagagc gtgatggaat ccggcgggct ggtgagcgac gacctcatca
     5521 tcaacctggt gaaggagcgc attgcccagc ccgattgcgc caatggcttc ttgttcgacg
     5581 gcttcccgcg caccatcccg caggccgagg ccatgaaggc cgccggcgtg aagctcgact
     5641 acgtgctgga aatcgatgtc cccttcgacg ccatcatcga gcgcatgagc ggccgccgca
     5701 gccaccccgc cagcggccgc acctaccacg tcaagttcaa cccgcccaag gccgagggag
     5761 tcgatgacgt caccggcgag ccgctggtgc agcgcgacga cgacaaggaa gacaccgtca
     5821 aaaagcgcct cgacgtctac acccagcaga cccgccccct ggtcgactac tacagccaat
     5881 gggccgccaa ggagccagcg gccgcgccca aataccgcaa gatcagcggc accggcagcg
     5941 tcgacgacat cacggcgcgc gcgctgcagg cgctggcaag ctgaaccgac ggttgcgtgg
     6001 agaacatcag gtccatggtt ctcctcgcct cgcagtcctt gccctagatg gcgttcagaa
     6061 acgaccgaat gagctcgctg tcggcgcgct ctgccatgca atacacgtag gtcgcggtgc
     6121 tggccggctc gcccgcgatg cgcacgggca cgaagcgggg gtcggcgatg aattcggcct
     6181 ccgacaccgc gcccagcccc agcccgcgcg ccacggcttc gcggatggct tcgcggctgc
     6241 cgatttccag atcggtgcgc ggttgcacgc cggcggcgcg cagggcgcct tccgcattgc
     6301 ggccgaggaa gcccaggcgc gttgggacaa ggcctgcaac gaattcttga aaaacgccct
     6361 ggtggtcgtg gtcatggccg ggtttttagc catcaagttc tggacgcctg ccaccccggc
     6421 acagcccgtt caggccacgc cgcccccggc agccgcccag gccgccaagc ccactgcgcc
     6481 acccgccgct gcgaaaacag caaaaaaacc cccgcccaaa ccgagcaagc cacgcacggt
     6541 ggagcagtgc ctgaaaatcg ccgacgagca cgtcatggtg cgctgcctgg agcgcgcgcc
     6601 ctagggtgtg aacgctctac gtgctccctg cc
//
LOCUS       DAOMVO010000121         6624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3111_length_6624_cov_12.073984, whole genome shotgun sequence.
ACCESSION   DAOMVO010000121 DAOMVO010000000
VERSION     DAOMVO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_6624_cov_12.073984"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKV30_07295"
     CDS             complement(<1..374)
                     /locus_tag="PKV30_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRL30054.1"
                     /translation="MNSTYSALPSQPSRSERDTFGPIDVPADRLWGAQTQRSLLNFDI
                     SGERQPRELIRALALVKRASAVVNQRLGLLDEKKSNAIVRAADEVLAGQHADEFPLVV
                     WQTGSGTQTNMNLNEVIANRAS"
     gene            600..1649
                     /locus_tag="PKV30_07300"
     CDS             600..1649
                     /locus_tag="PKV30_07300"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL30055.1"
                     /translation="MSTFETLARDLATRFDLGNQATPFLRMLMAHMTNPDKGGLPGFV
                     QSLRSAGWNTLESWLGRADLATNATEEHIERAFGHNGGLIGNITGKLGLRRDTIVRAL
                     AFAVPALLGRLTPGGTVPTVLPPEATNYIGDRREWIVPATAVAAAPVAAARGGMGWLP
                     WVIAALVAALLLGWCAMQKKPADMPAAPATPASTTDAPATPTTPAPSAPVENTVPAPS
                     GTTPPPIVQATEEPAGAGILAWRLDDMPALKVYFDSGSTTITPEFNDKAKDLVDYLAA
                     NSGAKAAISGFNDPTGDPVKNGELSKNRAQTVQAALVSAGVAADRLQLRKPADTTGTA
                     ATNAASSRVEVTVFK"
     gene            1818..3371
                     /locus_tag="PKV30_07305"
     CDS             1818..3371
                     /locus_tag="PKV30_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRL30056.1"
                     /translation="MPTTIRQEDLIESVAAALQYISYYHPADYIAHLARAYEREQSPA
                     AKDAMAQILTNSKMSATGHRPICQDTGIVNVFLKVGMDVRWEGFTGSLDDAINEGVRR
                     GYNHPDNTLRASVVADPQFARKNTKDNTPAVIVTEIVPGNTVDVTVAAKGGGSENKSK
                     MAMLNPSDSIVDWVLKTLPTMGAGWCPPGMLGIGIGGTAEKAVLLAKQSLMDDLDMYE
                     LQAKAAKGDALSDVEKLRLELFDKVNALGIGAQGLGGLTTVLDVKIALYPTHAASKPV
                     AMIPNCAATRHAHFVLDGSGPVYLDPPSLDLWPKVEWAPDTEKSQRVNLDTLTKEEVA
                     SWKPGQTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVGDEAV
                     GPAGPTTATRMDKFTDMMLSQTGLIAMIGKAERGPTAIEAIKQHKSAYLMAVGGAAYL
                     VSKAIKTAKVVGFEDLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWEKRIASGE
                     FKGISLATA"
     gene            3368..4186
                     /gene="murI"
                     /locus_tag="PKV30_07310"
     CDS             3368..4186
                     /gene="murI"
                     /locus_tag="PKV30_07310"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRL30057.1"
                     /translation="MTTASVPRADAPIGVFDSGIGGLSVLRALRAALPHDDFVYFADS
                     AHAPYGERDDAHVQARSRAVLAELQAAHGIKAFVIACNTATAAAAEALRAAHPDQVIV
                     GIEPALKPALTLTRTGRIGVLATRGTLASPKFRALHHGLATRAEFVLQPCDGLANAIE
                     KEAACADGTSARALIDQYLSALGPLGADPGAIDTLVLGCTHYQLARQHLLASLAGRAV
                     TLVDPADAVASHARTRLAEAGLARPAVDRAPGAVRLCASGADAALHAAAARWLP"
     gene            complement(4267..5568)
                     /locus_tag="PKV30_07315"
     CDS             complement(4267..5568)
                     /locus_tag="PKV30_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30058.1"
                     /translation="MALFSSDVLNPGVQRRELFGWAMYDFANSGYTTVVITAVFAAYF
                     VGGVAGGADWAPLAWTVALSISYAIVMLTMPSLGAWADLHGAKKKLLIWSTAGCVVAT
                     AAMALVRPGGVLLAMVLIIVSNTFYSYGESLTAAFLPELARPESMGKVSGWGWGFGYL
                     GGMLTLVGCLAYVLHAQGQGQGASHFVPVTLLITAVVYALAASVTFALLRERAQGVSS
                     DGAASSVGAGFTRLKQTFGEARRYGDFIWLLACTVAYQGGVAVAITLAAIYAEQVIGF
                     LPQETMVLILVLNVAAAAGAFGGGHLQDRIGHKLTLGLTLLGWIATCVIAALAATKGQ
                     FWVAATIAGLSMGSCQSVGRAMVGLFAPRAQLAEFFGLWSFATRLASIVGPLAYGLIT
                     WGTGGNQRVAIACTTLLFVLGLMALAPIDVVRGRAMGQQKL"
     gene            complement(5658..6284)
                     /locus_tag="PKV30_07320"
     CDS             complement(5658..6284)
                     /locus_tag="PKV30_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30059.1"
                     /translation="MNHEDAPFEIHVHGDVKLRPDVGFDALQDALKPLWKYAGARSLA
                     DGAASLYEDEPGIRFDPQQHLLQMCWTLPGEDNFRHQLDDLCMNLNELSAEGSQIEVT
                     FYDAEFDDEDEEAERESRDDFVLLFVGPTPEAIMQAQRNLLVEDVVALMERHFDGAEL
                     GGVVSEIDKLFEGRYSALTSSLELGKPPRGPGSGTPGHGGGRRPRHLH"
     gene            complement(6301..6393)
                     /locus_tag="PKV30_07325"
     tRNA            complement(6301..6393)
                     /locus_tag="PKV30_07325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6357..6359),aa:Leu,seq:taa)
     gene            complement(6464..6537)
                     /locus_tag="PKV30_07330"
     tRNA            complement(6464..6537)
                     /locus_tag="PKV30_07330"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6503..6505),aa:Cys,seq:gca)
BASE COUNT         1201 a         2336 c         2121 g          966 t
ORIGIN      
        1 tcgctggcgc ggttggcgat gacctcgttg aggttcatgt tggtctgcgt gcccgagccg
       61 gtttgccaga ccacgagcgg aaactcgtcc gcgtgctggc cggccagcac ctcgtcggcg
      121 gcgcgcacga tggcgttcga tttcttctca tccagcaaac ccagccgctg attgaccacc
      181 gccgacgcgc gcttgaccag cgccagcgcg cggatgagct cgcgcggctg gcgctcgccg
      241 gagatgtcga aattgagcag cgagcgctgg gtttgcgcgc cccacagccg gtcggccggc
      301 acgtcaatgg ggccgaaggt gtcgcgctcg ctgcggctgg gttgggatgg cagggcggaa
      361 taagtcgaat tcatggtcaa gggtcgttct tgatggttcg ggcgcgcgca ttggcgctct
      421 tgtcctacaa aaggtgcagc gccggcgcat ggctgccgct ctgtgcgctg catagacggg
      481 cctgcgtcgc acggcgatgg cgacacccgc gtcacatcac cacgaggcag ccattgtgcg
      541 cagcgagggg ctgcggcgca attgccatac gaccaaccgg gaaaacagga gagtccacca
      601 tgtccacctt tgaaacgctt gcacgcgatc tggccacgcg ctttgatctc ggcaaccagg
      661 ccacgccctt cttgcgcatg ttgatggcgc acatgaccaa ccccgacaag ggcggtttgc
      721 ccggcttcgt gcaaagcttg cgcagcgccg gctggaacac gctcgagagc tggctcggcc
      781 gcgccgacct ggccaccaac gccaccgaag agcacattga acgcgcgttt ggccacaacg
      841 gcggcctgat cggcaacatc accggcaagc tgggcttgcg ccgcgacacc atcgtgcgcg
      901 ccctcgcctt tgccgtgccc gcgctgctgg gccgcctgac ccccggcggc acggtgccca
      961 ccgtgctgcc gcccgaagcc accaactaca ttggcgaccg ccgcgaatgg atcgtgccgg
     1021 cgacggccgt ggctgcggcc ccggtggctg cggcgcgcgg cggcatgggc tggctgccgt
     1081 gggtcatcgc cgcgctggtg gccgccttgc tgctgggctg gtgcgccatg cagaagaagc
     1141 cggccgacat gcctgccgcg cctgcgacac ccgcttcgac cacggacgcg cccgccaccc
     1201 ccacgacccc cgcgccctcg gccccggtcg aaaacacggt gccggccccc agcggcacca
     1261 cgccgccgcc gatcgtgcaa gccaccgaag agccggccgg cgccggcatc ctggcctggc
     1321 gcctggacga catgcccgcg ctcaaggtct acttcgacag cggcagcacc accatcacgc
     1381 ctgagttcaa cgacaaggcc aaggacctgg tcgactacct ggccgccaac agcggtgcca
     1441 aggccgccat ttcgggcttc aacgatccga cgggcgaccc ggtgaagaac ggcgagctgt
     1501 ccaagaaccg cgcccaaacc gtgcaggccg ctctggtgtc ggccggtgtc gctgccgatc
     1561 gcctgcaact gcgcaagccg gccgacacca ccggcaccgc cgccaccaac gccgcctcga
     1621 gccgcgtgga agtgacggtg ttcaagtaaa gcgcccttga gcgtgaagcg cgcaccgccg
     1681 cgcttcaacg ccccaacgaa aaagctcgcg ccagtgtgcg ggctttttgt cgtcccgcac
     1741 ggcttgcgcc gccgtcaggc gcctggacgc gagccggccg ataattccgg ctttgctttc
     1801 ccaccccact tcgcgccatg cccacgacca tccgccaaga agacctgatc gaatccgttg
     1861 ccgccgcgct gcagtacatc agctactacc acccggccga ctacatcgcc cacctggcgc
     1921 gcgcctacga gcgcgagcaa tcgcccgccg ccaaggacgc gatggcgcag atcctcacca
     1981 acagcaagat gagcgccacc ggccaccggc cgatctgcca ggacaccggc atcgtcaacg
     2041 tgttcctgaa agtcggcatg gacgtgcgct gggaaggctt caccggcagc cttgacgacg
     2101 ccatcaacga aggcgtgcgc cgcggctaca accacccgga caacacgctg cgcgcttccg
     2161 tcgtcgccga cccgcagttc gcgcgcaaga acaccaagga caacacaccc gccgtcatcg
     2221 tgaccgagat cgtgcccggc aacacggtcg atgtgaccgt ggccgccaag ggcggcggct
     2281 ctgaaaacaa gagcaagatg gcgatgctca accccagcga cagcatcgtc gactgggtgc
     2341 tgaaaaccct gcccaccatg ggcgccggct ggtgcccgcc gggcatgctg ggcattggca
     2401 tcggcggcac ggccgaaaaa gccgtgctgc tggccaagca aagcctgatg gacgacctgg
     2461 acatgtacga attgcaggcc aaggcggcca agggcgatgc gctgtccgac gtcgaaaagc
     2521 tgcggctgga gctgttcgac aaggtcaacg ccctcggcat cggcgcgcaa gggctgggcg
     2581 ggctgacgac ggtgctggac gtgaagatcg ccctgtaccc cacgcacgcc gcttccaaac
     2641 ctgtagcaat gatccccaac tgcgcggcga cccgccacgc acatttcgtg ctggatggca
     2701 gcggcccggt ctacctcgac ccgccctcgc tcgacctgtg gcccaaggtc gaatgggcgc
     2761 ccgacaccga gaagagccag cgcgtcaacc tggacacgct gaccaaggaa gaagtcgcca
     2821 gctggaagcc cggccagacc ctgctgctga acggcaagat gctcaccggc cgcgacgccg
     2881 cacacaagcg catccaggac atgctggcca agggcgagaa gctgccggtc gatttcacca
     2941 accgcgtcat ctactatgtc ggcccggtcg atccggtggg cgatgaagcc gtgggccccg
     3001 ccggccccac caccgccacg cgcatggaca aattcaccga catgatgctg tcgcaaaccg
     3061 gcctgatcgc catgatcggc aaggccgagc gcggccccac cgcgatcgag gcgatcaagc
     3121 agcacaagtc ggcgtacctg atggccgtgg gcggcgcggc ctacctggtc agcaaggcca
     3181 tcaagaccgc caaggtggtc ggctttgaag acctggggat ggaagccatc tacgaattcg
     3241 acgtggtgga tatgcccgtc accgtggcgg tggacgccgg cggcaccagc gcgcacatca
     3301 ccggcccggc cgagtgggaa aagcgcatcg cctcgggtga gttcaagggc atctcgctcg
     3361 ccaccgcttg accactgcat ccgtgccgcg tgccgacgcg cccatcggcg tgttcgacag
     3421 cggcatcggt ggcctgtcgg tgttgcgcgc gctgcgggcc gcgctgccgc acgatgactt
     3481 cgtctacttc gccgacagcg cgcatgcgcc ctatggcgag cgcgatgacg cgcacgtgca
     3541 ggcgcgctcg cgtgccgtgc tggccgagct gcaagccgcg cacggcatca aggccttcgt
     3601 catcgcctgc aacaccgcta cggcggccgc ggccgaggcg ctgcgcgcgg cgcacccgga
     3661 ccaggtcatc gtcggcatcg agccagcgct caagcccgcg ctgacgctca cgcgcaccgg
     3721 gcgcatcggc gtgctcgcca cgcgcggcac gctggccagc cctaagtttc gcgccctgca
     3781 ccacggcctg gcgacacgcg ccgagttcgt gctgcagccg tgcgacggac tggcgaatgc
     3841 tatagaaaaa gaagctgctt gcgcagatgg gacaagcgcc agggccttga ttgatcaata
     3901 tctttcggcg ctcggcccgc tgggcgccga cccgggcgcc atcgacacgc tggtgctcgg
     3961 ctgcacgcac taccagctgg cgcggcagca cctgctggcc agcctggccg gccgcgcggt
     4021 cacgctggtc gatccggccg acgccgtggc gagccatgcg cgcacgcgtc tggccgaggc
     4081 cggtttggca cgcccggccg ttgatcgcgc accgggcgct gtgcggctgt gcgcctcggg
     4141 cgccgatgcg gcgctgcatg cggcggccgc gcgctggctc ccctgaaaca tcagcggccc
     4201 agcgcacgca tacatgatca aaaatggctg cagcgcacgc cgaacaagcg caagcagctc
     4261 ctctttttat agtttttgtt gacccatggc gcgtccgcgc accacgtcaa tgggcgccag
     4321 cgccatcagc cccaacacga acaacagcgt ggtgcaggca atcgccacgc gctggttgcc
     4381 gccggtgccc caggtgatca ggccgtaggc cagcggcccg acgatgctgg ccagccgggt
     4441 ggcaaagctc cacaagccga agaattcagc cagctgggct cgcggcgcga acagccccac
     4501 catggcacgc ccgaccgact ggcacgagcc catgctcagg cccgcaatcg tcgccgcgac
     4561 ccagaactga cccttggtcg ccgccagcgc ggcgatgacg caggtggcga tccagcccag
     4621 cagcgtcagg cccagcgtca gcttgtggcc gatgcgatct tgcaaatgac cgcccccaaa
     4681 cgcacccgcg gcggcggcga cgttgagcac caggatgagc accatggttt cctgcggcag
     4741 gaagccgatc acctgctcgg cataaatcgc cgccagcgtg atcgccaccg ccacgccgcc
     4801 ctggtaggcc acggtgcagg ccagcagcca gatgaagtcg ccgtagcggc gcgcctcgcc
     4861 aaaggtctgc ttcaaccgcg tgaacccggc gccaacggaa ctcgccgcgc cgtcggatga
     4921 cacgccctgc gcccgctcgc gcagcagcgc gaaggtgacg ctagcagcca gcgcatacac
     4981 caccgccgtg atcagcagcg tgaccggcac gaaatgcgac gcgccctgcc cctggccttg
     5041 cgcatgcagc acatacgcca ggcagccgac cagcgtcagc atgccgccca gatagccgaa
     5101 accccagccc cagccgctga ccttgcccat ggattcgggc cgcgccagct cggggaggaa
     5161 ggccgcggtc agcgactcgc catacgagta gaaggtgttg gagacgatga tgagcaccat
     5221 cgccagcagc acgccgccgg gacgcaccag cgccatcgct gcagtggcga cgacgcaacc
     5281 cgccgtggac cagatcagca gctttttctt cgcgccatgc aggtcggccc acgcgcccag
     5341 gctgggcatg gtgagcatga cgatggcgta ggaaatggac agcgccaccg tccacgccag
     5401 cggcgcccag tcggccccgc ccgccacgcc gcccacgaag taggccgcaa acaccgccgt
     5461 gatgaccacg gtggtgtagc ccgagttggc gaagtcgtac atcgcccagc cgaacagctc
     5521 gcgccgctgc acgccggggt tcagcacgtc ggacgagaac aaggccatgc tgcgcgtctc
     5581 cagccggcgc catcggccgg ccagcgcgca gcatagcaat cagggatgac gcgccggccg
     5641 cgtgggccgg cttggcgtca gtgcaggtgg cgcgggcgcc ggccgccgcc gtgccccggc
     5701 gtgccgctgc ccggcccgcg cggcggcttg cccagttcaa gcgaactggt gagcgccgaa
     5761 tagcggcctt cgaacagctt gtcgatctcg ctgaccacgc cgccgagttc ggcgccgtcg
     5821 aaatggcgct ccatcagcgc gaccacgtcc tcgaccagca gattgcgttg cgcctgcatg
     5881 atcgcctcgg gcgtcgggcc gacaaacagc agcacgaagt cgtcgcgcga ctcgcgctcg
     5941 gcctcttcgt cctcgtcgtc gaactcggca tcgtagaagg tgacttcgat ctgcgagcct
     6001 tcggccgaca gctcgttcag gttcatgcac agatcgtcga gctgatggcg aaaattgtct
     6061 tcgcccggca aggtccagca catctgcaac aggtgctgct gtgggtcgaa gcgaatgccc
     6121 ggctcgtcct cataaaggct ggccgcacca tcggccagcg agcgggcgcc agcgtacttc
     6181 cagaggggtt tgagggcatc ctgcagggcg tcgaagccca cgtcgggacg aagtttcacg
     6241 tcgccatgca cgtgaatctc gaaaggtgca tcttcatgat tcatggcgtt tttccttctc
     6301 tggtgccccg ggccgggatc gaaccggcac gccccctttc gggaaagcgg cggattttaa
     6361 gtccgctgtg tctaccagtt tcaccaccgg ggccaagcgt gcattgtcgc aggctcggcg
     6421 ggccgccgcg cggtgacaaa aaagtgagtg ggggaagaaa aagtggaggc gcgatccgga
     6481 gtcgaaccgg actagacgga tttgcaatcc gttgcataac cgctttgcta tcgcgccgaa
     6541 gggaatacaa aaaaaggaag ccaagcttcc ttttgatgca gccgccggct tggtgaccgg
     6601 ctgcgaattt tggagcggga aacg
//
LOCUS       DAOMVO010000122         6592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3130_length_6592_cov_12.028453, whole genome shotgun sequence.
ACCESSION   DAOMVO010000122 DAOMVO010000000
VERSION     DAOMVO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3130_length_6592_cov_12.028453"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKV30_07335"
     CDS             complement(<1..922)
                     /locus_tag="PKV30_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30060.1"
                     /translation="MSDSAAATPAAAPPAERRPSWLASLKIYLQPTSLRMLALGFAAG
                     LPLLLVLGTLSFRLREAGIDRSTIGYLSWVGLAYGFKWAWAPLVDRVPLPGLNAWLGR
                     RRSWLLLSQLGVAAGLVGMALADPRAGLTAVVWCALAVAFASATQDIALDAYRIESAP
                     TEQQPALAASYQTGYRLAMIWAGAGVLWLAARAQGGTAGYDNHAWQVAYLVMAASMAL
                     GVLTVLLSPEPAPRVIAPARNAAEWVRSALVEPFADFFKRYKWQAALILALIAVYRIS
                     DVVMGIMANPFYVDMGYTKDEVAAVTKIYGV"
     gene            1014..1961
                     /locus_tag="PKV30_07340"
     CDS             1014..1961
                     /locus_tag="PKV30_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRL30061.1"
                     /translation="MCWICHAKTSHPAAADRPPWSPRRAFLLAAGSAVAAPVLAQVDV
                     GRSSSMRNLVPAEQLEQAAQQQYAQLLAEARAKGLLAGDGNPQLRRLRAIAQRLIPYT
                     AQWNERARNWRWEVNLFNSKSINAFCMPGGKIGFFTGILDQLKLSDDEVAMIMGHEMA
                     HALREHARERMAKTTATSLGLEVAASLFGLGQLGQLGANVGSQLAALKYSRDDETEAD
                     LVGLEIAARGGYNPHAAVSLWNKMTAASQRQGGTSFFSSHPSGPDRTRRLQDSIPRVE
                     GLYRQARRGAAAAPAPTQVPAPSGEPRFDTPVGRTMDAR"
     gene            1977..2612
                     /locus_tag="PKV30_07345"
     CDS             1977..2612
                     /locus_tag="PKV30_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL30062.1"
                     /translation="MPTSPLDAVSAYYSGTLQRFGATPLGVDWPNRLNQELRLVQLLK
                     VCDLSAPRSLNDVGCGYGALRTLLSRRHRRAQIDLLGTDVSPAMVAAARRRWRHRADC
                     AFEVAEGAVRLADYSLASGIFNVKLACPLPEWEALVAGTLDNLKRHSRLGYAVNFIAP
                     PAPGQASPPELYRPAAERWLDHIADTQAGAEVTLLRGYGLPEYTLLVRPAH"
     gene            2793..3311
                     /locus_tag="PKV30_07350"
     CDS             2793..3311
                     /locus_tag="PKV30_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30063.1"
                     /translation="MGYFMAYADIQIPSLLDQLNQRFAPAQPDLGGESLYGGIAEMAE
                     IQKEFAIFKKGRPLQQSMRALNLNAGNNAVRQRWLTLIEGLAKHPSNRDGENGGDAIV
                     NALVENFAAKEPLPVFFTSHDMQGKPVKESPVLITPGARPLHYLETDYLVISLPMQSR
                     QAADRMRAQRPT"
     gene            complement(3308..6493)
                     /locus_tag="PKV30_07355"
     CDS             complement(3308..6493)
                     /locus_tag="PKV30_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRL30064.1"
                     /translation="MPAGAKGLNVGGRRFCTHCGADSRDTDRFCAACGQTLSAAPAAW
                     GEVKPATILFADIVDSTRQIAELSPEQAMRQLQPAVERMVAVVEQHGGTVLRTLGDGI
                     MALFGVPRALEQHAEQACLAAVQMQRVAGTQGDDGSPRLALRIGIHTGKVASDPTEAG
                     DRRGGGAHGVAIHLASRVAAQAEPGQVLLTDDTRHLLRPGLLAARPVGAWHLKGILQP
                     VPLFALRLDVASAAVPPVASAGGRFLGRADELAQLTHALGRTRAGQGQVVCISGNAGL
                     GKTRLCAEFAQRCERAGMRVVRVQAHPLSHALPLRPARELLGALCLDCPPDLAPPEAR
                     RRIDAALAQASQPSPVNAFLLHELMGVTDDDAPPPAGLPPFRDARRVRLLSLIAQLVR
                     ADAASLRLLVVDDLHWLDAASSPILATVADAVADTHTLLLVNQRGSAPPWPRLPHAAM
                     LALGTLAPAALRGIVADRLSALAEPGARATTIAADDRAGWVDRVTERSQGNPFFAEEL
                     ARHLRRTGADPARWADELPDSIDGLIAARVDALPAADKRVLQTCAVIGKHVDAAVLAR
                     VLRPAPGTLAGALRRLCAAELLARAPDGTGLHFAHPLIQEGAYAAQLRSRTQGMHARV
                     AEVMEQRYVRAPRAGELAALMAHHREQGGDRLLAARHAVRAAQWLRAGDAAQVIAAWR
                     KVMTLLESEAETEETRQLSALAAGRIVFVGWRGGITTQEVSQLVTRALAQAAAADARL
                     PQLLHFAHARMLQASGGSADDYVHAVRQVLDMPDPPGDTGGRRALLNVALSQAYGWAG
                     LLREGLAASDAALAGLHRITPFDVDFIGYGVSHWAWSLRSRLLLRMGRLAEAKACNDR
                     VQTMLNDEPDVVIQGIFQTLRLEHDCMQARWRRAWADTQRFDLAARERNDYLRISGAY
                     FLSLVDIARHQYTSACARLHDGLQHVRRAGVAVDFEPEIGVLLAEALAARRAWPEAAR
                     AAQEAHAVAHQRANRIAECRAALVLARAALQGVSAPSSAAEPAPSSAPEPAPLCWLTH
                     AGELLDLTGADFWRPAWRAVSARSRASARAA"
BASE COUNT         1014 a         2406 c         2280 g          892 t
ORIGIN      
        1 tgacgccgta gatcttggtc accgccgcca cctcgtcctt ggtgtagccc atgtcgacgt
       61 agaacgggtt ggccatgatg cccatcacca catcgctgat gcggtagacg gcgatcagcg
      121 ccaggatcag cgccgcctgc cacttgtagc gcttgaagaa gtcggcaaac ggctcgacca
      181 gcgccgagcg cacccattcg gcggcgttgc gtgccggcgc aatcacgcgc ggcgccggct
      241 cgggcgacag cagcaccgtc agcacgccca gcgccatgct ggcggccatg accaggtagg
      301 cgacttgcca ggcgtggttg tcgtaacccg ccgtgccgcc ttgcgcgcgc gcggccagcc
      361 acagcacgcc ggcgccggcc cagatcatgg ccagccgata acccgtctgg tagctggccg
      421 ccagcgcggg ctgctgctcg gtcggggccg attcgatgcg gtaggcgtcc agcgcgatgt
      481 cttgcgtggc cgatgcaaac gccaccgcca gcgcgcacca caccaccgcc gtcagcccgg
      541 cgcgcgggtc ggccagcgcc atgccaacca gccccgccgc cacgcccagt tgcgacaaca
      601 gcagccagct gcgccgccgc cccagccacg cgttaagccc cggcagcggc acgcgatcga
      661 ccagcggcgc ccacgcccac ttgaagccgt aggccagccc gacccagctc aaataaccga
      721 tggtgctgcg gtcgatgccc gcctcgcgca atcgaaatga gagcgtgccc agcaccagca
      781 gcaagggcaa gccggccgca aagcccaaag ccagcatgcg caggctggtc ggctgcaggt
      841 agatcttcag gctggccagc cacgacggcc ggcgctcggc cggcggtgcg gcggcgggcg
      901 tggccgcggc gctgtcagac aaggactgca cccgtttgcg ccgccagcgg ggtggcgagc
      961 gggcggcact gcggtatctt gcggctgttc atccgcccta ttattcaccc gccatgtgct
     1021 ggatttgcca cgccaagact tcgcaccccg ccgccgctga ccgcccgccg tggtcgccgc
     1081 gccgcgcctt tctgctggcc gctggcagcg ccgttgccgc gccggtgctg gcgcaggtcg
     1141 atgtcggccg ctcgtccagc atgcgcaacc tggtaccggc cgagcaactg gagcaagcgg
     1201 cgcagcagca atacgcgcaa ctgttggccg aagcgcgcgc caagggcctg ctggccggcg
     1261 acggcaaccc gcagctgcgg cgcctgcgcg ccatcgcgca gcggctgatc ccctacaccg
     1321 cgcagtggaa cgagcgcgcg cgcaactggc gctgggaagt caacctgttc aacagcaagt
     1381 cgatcaacgc cttctgcatg ccgggcggca agatcggctt cttcaccggc atccttgatc
     1441 agctcaagct gagcgacgac gaagtcgcca tgatcatggg ccacgaaatg gcccacgccc
     1501 tgcgcgaaca cgcgcgcgag cgcatggcca agaccaccgc caccagcctc gggctggaag
     1561 tggcggcctc gctgttcggc ctcggccagc tggggcagct gggcgccaac gttggctcgc
     1621 agctggccgc cctcaagtac agccgcgacg acgagaccga ggccgacctg gtcggcctgg
     1681 aaatcgccgc ccgcggcggc tacaaccccc atgccgccgt cagcctgtgg aacaaaatga
     1741 ccgccgccag ccagcgccag ggcggcacca gttttttctc aagccatccg agcgggcccg
     1801 accgcacacg ccggctgcaa gacagcattc cgcgcgtcga agggctgtac cggcaggcgc
     1861 ggcgcggcgc cgctgcggcg ccagcgccca cgcaagtgcc agcgcccagc ggcgagccgc
     1921 gcttcgacac cccggtcggg cgcaccatgg acgcgcgctg acggcgccgc ccccgcgtgc
     1981 cgacctcgcc gctcgacgcc gtcagcgcct actacagcgg caccttgcag cgctttggcg
     2041 ccacgccgct gggcgtggac tggccgaacc ggctgaacca ggaactgcgc ctggtgcagc
     2101 tgttgaaagt gtgcgacttg tcggccccgc gctcgctcaa cgatgtggga tgcggctacg
     2161 gcgcgttgcg cacgctgttg agccgccgcc accgccgtgc gcaaatcgac ctgctgggca
     2221 ccgacgtatc ccccgccatg gtcgccgccg cgcgccgccg ctggcgccac cgggcggatt
     2281 gcgccttcga ggtggctgaa ggcgccgtgc gcctggccga ctattcgctg gccagcggca
     2341 tcttcaacgt caagctggct tgcccgctgc ccgagtggga agcgctggtg gccggcacgc
     2401 tggacaacct taagcgccac agccggctgg gctacgccgt caacttcatc gctccgcccg
     2461 cgcccggcca ggccagcccg cccgagctgt accgcccggc cgccgaacgc tggctggatc
     2521 acatcgccga cacgcaagcg ggcgctgaag tcacgctgtt gcgcggctat gggctgccgg
     2581 aatacacgct gctggtgcgg cccgcccact gacgcagctg ccatttctat tgttttgata
     2641 gctgcttgcg cttgtcagac ttgcgctgga ggtctttttt atcaacaatt cgcgggcgct
     2701 gaaacgctac cccgtttgtg cttgccgccg gtttgcggcg catgcaaaag tttgcacctt
     2761 tcaaacacca ccgcgaacag aggagagata ccgtgggcta tttcatggcg tatgccgaca
     2821 tccagatccc cagcctgctg gaccaactca accagcggtt tgcgccggca cagcccgacc
     2881 ttggcgggga gagcctgtac ggcggcatcg ccgagatggc cgagatccaa aaggaattcg
     2941 ccattttcaa gaaaggccgg ccactgcagc aaagcatgcg tgcgctcaac ctgaatgcgg
     3001 gcaacaacgc cgtgcgccag cgctggctga cgctgatcga gggcttggcc aagcaccctt
     3061 ccaaccgcga cggtgagaac gggggcgatg cgatcgtcaa cgcgctggtg gaaaactttg
     3121 ccgccaagga gccgctgccc gtgttcttca cctcgcacga catgcagggc aagccggtca
     3181 aggaaagccc ggtgctcatc acccccggcg cgcgcccgct gcactatctg gaaacggact
     3241 acctggtgat ttcgctgccc atgcagtccc ggcaagccgc cgaccgcatg cgcgcgcagc
     3301 gcccgaccta agccgcgcgc gccgacgccc ggctgcgtgc cgacacggcg cgccacgccg
     3361 ggcgccagaa gtcggcgccc gtgaggtcca gcagttcgcc cgcatgcgtc agccagcaaa
     3421 ggggcgccgg ctcgggggcg ctgctcggcg ccggctcggc agcgctgctc ggcgccgaca
     3481 cgccctgcag cgcagcgcgc gccagcacca gcgcggcgcg acattcggcg atgcggttgg
     3541 cgcgttggtg cgccacggcg tgggcctcct gcgcggcgcg tgcggcctcg ggccaggcgc
     3601 gccgcgcagc cagcgcttcg gccagcagca cgccgatctc gggctcgaaa tccaccgcca
     3661 cgccggcccg gcgcacgtgc tgcaagccat cgtgcaagcg cgcgcaagcg ctggtgtact
     3721 ggtggcgcgc gatgtccacc aacgacagaa agtaggcgcc cgagatgcgc aggtagtcgt
     3781 tgcgctcgcg cgctgcaaga tcgaaccgct gcgtgtccgc ccaggctcgc cgccagcgcg
     3841 cctgcataca gtcgtgctcg aggcgcaatg tctgaaagat gccctggatc acgacatccg
     3901 gttcgtcgtt cagcattgtc tgcacgcggt cgttgcatgc cttggcttcg gccaggcggc
     3961 ccatgcgcaa cagcaggcgc gagcgcaggc tccacgccca gtgcgacacg ccataaccga
     4021 tgaaatcgac gtcgaagggc gtgatgcggt gcagccccgc cagcgccgcg tcgctggcgg
     4081 ccagcccctc gcgcagcagg ccggcccagc cgtaggcctg cgacagcgcg acgttcagca
     4141 gcgctcgacg gcccccggtg tcgcccggcg ggtcgggcat gtccagcacc tgccgcaccg
     4201 catgcacgta atcgtcggcc gagccgccgc ttgcctgcag catgcgcgca tgggcaaagt
     4261 gcagcagctg cggcaggcgc gcgtcggctg cggcggcctg cgccaacgcc cgggtgacga
     4321 gttgcgacac ctcctgcgtg gtgatgccgc cgcgccaacc cacaaacacg atgcggccag
     4381 cggccagcgc gctgagctgg cgcgtttcct cggtttccgc ctcgctttcc agcagcgtca
     4441 tcaccttgcg ccaggcggca atgacctgcg ccgcgtcgcc agcgcgcagc cattgcgcgg
     4501 cgcgcacggc gtggcgagca gccagcagcc ggtcaccccc ctgctcgcgg tggtgcgcca
     4561 tcagcgctgc cagttcgcca gcacgcggtg cacgcacgta gcgctgctcc atgacctcgg
     4621 ccacccgcgc gtgcatgccc tgcgtgcggc tgcgcagttg cgcggcgtag gcgccttcct
     4681 gaatcagcgg atgagcgaag tgcaggccag tgccgtccgg cgcgcgcgcc agcagctcgg
     4741 ccgcgcacag gcgccgcagc gccccggcca gcgtgcccgg cgccgggcgc agcacgcgcg
     4801 ccagcaccgc ggcatcgacg tgcttgccga tgacggcgca agtctgcagc acgcgcttgt
     4861 cggcggcggg caaggcatcg acgcgagcgg cgatcaggcc gtcgatgctg tcgggcagtt
     4921 cgtcggccca gcgcgccggg tcggcgccgg tgcggcgcag gtggcgcgcc agttcttcgg
     4981 caaagaaagg gttgccctgg ctgcgctcgg tcacgcggtc gacccagccg gcgcggtcgt
     5041 cggcggcgat ggtggtggcg cgcgcgccgg gctcggccag cgccgacagc cggtcggcca
     5101 cgatgccgcg cagcgccgcc ggcgccagcg tacccagcgc cagcatggcg gcgtgcggca
     5161 ggcgcggcca gggcggtgcg ctgccgcgct ggttgaccag cagcagcgtg tgggtgtcgg
     5221 ccacggcgtc ggccacggtg gccaggatgg ggctgctggc ggcatcaagc cagtgcaggt
     5281 cgtccaccac cagcaggcgc aggctggcgg cgtcggcgcg caccagttgc gcgatgagcg
     5341 acagcaggcg cacccggcgc gcatcgcgaa acggcggcag gccagcgggc ggcggcgcgt
     5401 cgtcgtcggt cacgcccatc agctcgtgca gcaagaaggc attgaccggg ctgggctggc
     5461 ttgcctgggc cagcgcggcg tcgatgcggc ggcgcgcctc gggcggcgcc aagtccggcg
     5521 ggcagtcgag gcacagcgcg ccgagcagct cgcgcgccgg gcgcaggggc agcgcgtggc
     5581 tcaaggggtg ggcctgcacg cgcaccacgc gcatgccggc gcgttcgcag cgctgcgcga
     5641 attcggcgca caggcgcgtc ttgcccaggc cggcgttgcc gctgatgcac accacctgcc
     5701 cctggccggc gcgcgtgcga ccgagcgcat gggtgagctg cgccagctca tcggcccggc
     5761 ccagaaaacg cccgccagcg ctggcgaccg ggggcacggc ggcgctggcc acgtccagcc
     5821 gcagcgcgaa cagcggcacc ggctgcagga tgcccttgag gtgccaggcg ccgaccggcc
     5881 gcgcggccag caggccgggg cgcagcagat ggcgcgtgtc gtcggtcagc agcacctgac
     5941 cgggttcggc ctgggcagcg acgcggctgg ccaggtgaat ggcgacgccg tgcgcgccgc
     6001 cgccgcgccg gtcgccggct tcggtcgggt cgctggcgac cttgccggtg tggatgccga
     6061 tgcgcagcgc caggcgcggg ctgccgtcgt caccctgcgt gccggccacg cgctgcatct
     6121 gcaccgccgc caggcaggcc tgctcggcgt gctgctcgag cgcgcgcggc acgccgaaca
     6181 gcgccatgat gccgtcgccc agcgtgcgca gcaccgtgcc gccgtgctgc tcgaccaccg
     6241 ccaccatgcg ctcgacggcg ggctgcagct ggcgcatggc ctgctcgggg ctcagctcgg
     6301 cgatctgacg cgtggaatcg acgatgtcgg caaacaggat ggtggccggc ttgacctcgc
     6361 cccaggcggc gggcgcggcg ctgagcgtct ggccgcaggc ggcgcaaaag cggtcggtgt
     6421 cgcgggagtc tgccccgcaa tgggtgcaaa aacggcgacc gcccacgttc agccccttgg
     6481 cgccggcggg cacgaacgcc taacggataa cgcggaaagc ggcaagcaca tggatggcat
     6541 gctaaccgca atcgcttacg ttctttacat caaccgacgg ccaatctgca tc
//
LOCUS       DAOMVO010000123         6507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3179_length_6507_cov_16.472567, whole genome shotgun sequence.
ACCESSION   DAOMVO010000123 DAOMVO010000000
VERSION     DAOMVO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3179_length_6507_cov_16.472567"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV30_07360"
     CDS             complement(<1..242)
                     /locus_tag="PKV30_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRL30065.1"
                     /translation="MTDSKSIAACAYSASARAELDVKPVPSPCINVCRMTPDRSHCEG
                     CFRTLDELRAWRDMDDAGKRAVWALIAQRSGGARVL"
     gene            complement(239..763)
                     /locus_tag="PKV30_07365"
     CDS             complement(239..763)
                     /locus_tag="PKV30_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRL30066.1"
                     /translation="MNSLEAAVNLPEGVRRVAAALQQAGHAHLPVMLDDAARTAQQAA
                     DALGIAVGQIAKSIIFRRKSDDAAVLVITSGDRRVDEKKVEALVCADGGGKLGRADAE
                     FVKAKTGFSIGGVSPLAHAEPPVTLIDRELFRFAEIWAAAGHPHGVFKLTPDDLQKLT
                     GAPVADVTQVAATA"
     gene            complement(766..1413)
                     /locus_tag="PKV30_07370"
     CDS             complement(766..1413)
                     /locus_tag="PKV30_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRL30067.1"
                     /translation="MDWHVWLAYFLAAWLIALSPGSGAVLSMSHGLQYGVRQTTATIV
                     GLQIGLASILLVAGAGVGALLLASTTAFMVVKVLGAGYLIWLGVKQWRAPVDGAASDA
                     AAPSEPAGLSVRQRLLRGALTNATNPKGIVFMVAVLPQFIDPNKPLALQLAILLVTTL
                     AVDSVVMHGYAFLASRLRALMQSVRARKTQNRVFGGVLMGMGASLLMVDRVSKAG"
     gene            complement(1427..2347)
                     /locus_tag="PKV30_07375"
     CDS             complement(1427..2347)
                     /locus_tag="PKV30_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRL30068.1"
                     /translation="MPLPTRAKIVDVGPRDGLQNEKQPVPAAVKIELVHKLQDAGLKE
                     IEVTSYVSPKWVPQMGDNAEVMAGVKRQSGVRYSVLTPNLKGFEAAIAGPRETWPDEI
                     VVFGAASEAFSQKNINCSIAESIERFAPVVEAARAKNIYVRGAMSCTVGCPYEGDIAP
                     SRVDYLAGLMKGIGVQHVGVADTIGVGTPRKVQAAIEATLKHYAIDDVSGHFHDTYGQ
                     ALANTLAALELGVWQYDTSVAGLGGCPYAKGATGNVATEDVVYMLHGLGLDTGIDLDK
                     LIDAGQYISDFLQRPSNSRVAKALLTKRAG"
     gene            2475..3239
                     /locus_tag="PKV30_07380"
     CDS             2475..3239
                     /locus_tag="PKV30_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRL30069.1"
                     /translation="MKLALLSDIHANRQALDACLAHAEAAGAERHAFLGDLVGYGADP
                     SYVVQRVMALAARGALVIQGNHDLYATQPVGDGKSLGHASVAWTQQQLSEDERAFLAA
                     LPLTAVDGSALLAHASADAPASWRYVENPQLAALSLDAASQPPVGNADVRYVFGGHVH
                     FQTLYFRGGAGKLMPFAPTPGVPIPVPRHRHWLATIGSVGQPRDGRIEAMYAIFDEAA
                     QQLTFHRVPYDVPAAAAAIRATPLPAFFADRLELGQ"
     gene            3236..4687
                     /locus_tag="PKV30_07385"
     CDS             3236..4687
                     /locus_tag="PKV30_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HRL30070.1"
                     /translation="MKSLVPNTVIDGFKLGEMRHKGGMALIYDVAYADGRAAPFPMVM
                     KVPRMTAGDGAETIVSFEVEHQMMQALDGPHVPHLVAAGDLSVLPYLVMEYVPGRTLD
                     LWLDQVNQGVLLAPETLAQLGAAVAHAAHSLHEQNAVHLDLKPANVIIKGDGTAVLLD
                     FGLSFHAHYPDLLAEETRHAVGSPAYMSPEQVVGVRGDPRSDVFAIGVMLYELATGEL
                     PFGAPTSQGGLRQRLWMDPQPPRARRPDLPEWLQEVILRCLEPAAAERYPSAAHLAFD
                     LTHPDQVQVTERGQRLKGTGAGTHIKRWLRAAGAEYQPSPLPARQIAEVPILMVAVPH
                     RDVTDATLYSLREAVGRSLGIRPGARLAVVTVVAPGAASTTDAGKSETALHRQMLAML
                     KNWTVGLKLDGHQVSYHVLEDGDPADAIVRYAETNRVGMIVMGAATHGLQLQRFVATV
                     PIKVAMRAPCTVILVKQTMPFAELHQAGAKSEV"
     gene            complement(4855..5781)
                     /locus_tag="PKV30_07390"
     CDS             complement(4855..5781)
                     /locus_tag="PKV30_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HRL30071.1"
                     /translation="MKLLLCNRDGRPSEAWLNDLRRALPEAQVDEWQPGVPADYDAAI
                     VWNPPQQLFDEQPQLNAVFNLGAGVDKLMALKLPPDLQIVRIEDAGMAVQMAEYVCHA
                     LIRFYREFDWYAQQQKEQLWKPRRPWERDDFPVGIMGLGALGARVAQAVRQFDFPVLG
                     WSRTRRDLEGVRCYAGKDELSAFLKATRVLVCLLPLTDETRGILNREALERVQLGGYL
                     INVARGALVVEDDLRNLLEEGHLAGATLDVFEDEPLPPDHWLWTQPFVNVTPHISAQT
                     VRRDAVVQVALGVRALMRGALPAGVVKRSHGY"
     gene            complement(5778..>6507)
                     /locus_tag="PKV30_07395"
     CDS             complement(5778..>6507)
                     /locus_tag="PKV30_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRL30072.1"
                     /translation="TNVQFLRRVLATESFSQGKLDTALIQRESEHLFNQDPLGVDMAV
                     AAAVARTVLAERDAESHDPFSRTDGFRSHGVASRRIDLEYAGQPVVAKLRYLDDGLQL
                     KVGDGESAPLSFTRQPDGAIDVRYAGQRQVVHTFPRGEVVHVFGTDGATTITELDVLA
                     HAVEGAGEGGRLTAPMPGKVVSIAVKAGDKVTKGQPLAVMEAMKMEHTIAAPQDGVVD
                     EVLYAPGDQVTEGAELLRLGMGAA"
BASE COUNT         1043 a         2287 c         2128 g         1049 t
ORIGIN      
        1 tccagcaccc gcgccccacc gctgcgctgg gcgatcagcg cccagacggc gcgtttgccg
       61 gcgtcgtcca tatcgcgcca ggcgcgcaat tcgtccagcg tgcgaaagca gccttcgcaa
      121 tggctgcggt cgggtgtcat gcggcacacg ttgatgcagg gcgaaggcac cggtttcaca
      181 tccaattcgg cgcgagcgct tgccgaataa gcgcaggcag ctatgctttt tgaatccgtc
      241 atgcagtggc cgcaacctgc gtcacatcgg ccaccggcgc gccggtgagc ttttgcagat
      301 catccggcgt cagcttgaag acgccgtgcg ggtggccagc agcggcccag atttcagcga
      361 agcggaatag ctcgcgatca atcagcgtca ccggcggctc ggcatgcgcg agtggcgaca
      421 caccgccaat cgagaaaccg gtcttggcct tcacgaactc ggcgtcggcg cggccgagct
      481 tgccgccgcc atcggcgcag accagcgcct cgaccttctt ctcgtccacg cgccggtcgc
      541 cgctggtgat gaccagcacc gccgcgtcgt cgctcttgcg gcgaaagatg atgctcttgg
      601 cgatctggcc gacggcgatg cccagcgcat cggccgcctg ctgcgcggtg cgcgcggcgt
      661 cgtcgagcat caccggcagg tgcgcgtggc cggcctgctg caaggcggcg gcgacgcggc
      721 gcacaccctc gggcaagttc actgctgctt caagactgtt catgctcaac ccgccttcga
      781 cacgcgatcg accatcagca ggctggcgcc catgcccatc agcaccccgc caaacacgcg
      841 gttctgcgtc ttgcgcgcgc gcaccgactg catcagcgcg cgcaggcgcg aggccagaaa
      901 ggcgtagccg tgcatcacca cgctatcgac cgcgagcgtg gtgaccagca gaatggccag
      961 ttgcagcgcc agcggcttgt tcggatcgat gaattgcggc agcacggcga ccatgaagac
     1021 gatgcccttg gggttggtcg cattggtcag cgcgccgcgc agcaggcgct ggcgcacact
     1081 gagcccggcc ggctcggacg gtgcggccgc atcgctcgcg gcgccatcca ccggcgcgcg
     1141 ccactgcttg acgcccagcc agatcaggta acccgcgccc agcaccttga ccaccataaa
     1201 ggccgtggtc gatgccagca gcagcgcgcc cacgccggcg ccggccacca gcaggatgga
     1261 cgccagcccg atttgcagcc cgacgatggt ggcggtggtt tgccgcacgc cgtattgcag
     1321 gccatggctc atcgacagca ccgcgcccga accgggcgac agcgcgatca gccacgcggc
     1381 caaaaagtaa gccagccaca catgccaatc catgctgccc ctccgatcaa ccggcgcgct
     1441 tggtcagcag cgccttggcc acgcgcgaat tcgacgggcg ctgcagaaag tcgctgatgt
     1501 actgaccggc gtcgatcagc ttgtcgagat caatgccggt gtctagcccc aagccgtgca
     1561 gcatgtagac cacgtcttcg gtcgctacgt ttccggtagc gcccttcgca tacggacagc
     1621 cgcccagccc cgcaaccgac gtgtcgtact gccacacgcc cagctccaac gcggccagcg
     1681 tgttggccag cgcctggccg taggtgtcgt ggaagtggcc ggacacatcg tcgatggcgt
     1741 agtgcttcaa ggtcgcctcg atcgcagcct gcaccttgcg cggcgtgccg acgccgatgg
     1801 tgtcggccac gccgacgtgc tgcacgccga tgcctttcat caggccggcg aggtagtcga
     1861 cgcgcgacgg tgcgatgtcg ccctcgtacg ggcagcccac ggtgcagctc atggcgccgc
     1921 gcacgtagat gttcttagcg cgcgcggcct cgaccacggg cgcaaagcgc tcgatgctct
     1981 cggcaatcga gcagttgatg ttcttctggc tgaaggcttc gctggcggcg ccgaagacga
     2041 cgatctcgtc gggccaagtc tcgcgcgggc cggcaatggc ggcctcgaag cccttcaggt
     2101 tgggcgtcag cacgctgtag cgcacgccgg actgccgctt cacgccggcc atcacctcgg
     2161 cgttgtcgcc catctgcggc acccacttgg ggctgacgta gctggtgacc tcgatctctt
     2221 tcaggccggc gtcctgcagc ttgtgcacga gctcgatctt gacggcggcg ggcaccggct
     2281 gcttctcgtt ttgcaagccg tcgcgcgggc cgacatcgac tatttttgcg cgggtgggca
     2341 ggggcatgat gggctttctc tgtcgcggaa tgggggcacc ggtcagttta ccgatttgat
     2401 ggtccgcctg cgcggcgcca gcgggcaggc ggcgcccgta tcatggattt ctcgccaccg
     2461 acttgcgccc cgccatgaag ctcgccctgc tgtccgacat ccacgccaat cggcaggcgc
     2521 tggacgcctg cctggctcac gccgaagcgg ccggcgccga gcgccatgcc tttctggggg
     2581 atctggtcgg ctacggcgcc gacccgtcgt acgtggtgca gcgcgtgatg gcgctggccg
     2641 cgcggggcgc gctggtcatt cagggcaacc acgacctgta cgccacccag ccggtgggcg
     2701 acggcaaaag cctgggtcac gccagcgtgg cctggacgca gcagcaactc agcgaagacg
     2761 agcgcgcctt tctggccgcg ctgccgctga ccgccgtcga cggcagcgcg ctgctggcgc
     2821 acgccagcgc cgacgcgccc gccagctggc gctatgtcga gaacccgcaa ctcgccgccc
     2881 tgagcctgga cgccgccagc cagccgccgg tgggcaacgc cgacgtgcgc tatgtgttcg
     2941 gcggccacgt gcattttcag acgctgtact ttcgcggcgg cgccggcaag ctgatgccgt
     3001 ttgcgccgac gccgggcgtg ccgattccgg tgccgcgcca ccggcattgg ctggccacca
     3061 tcggctcggt cggccagccg cgcgacgggc gcatcgaggc catgtacgcc atcttcgacg
     3121 aagcggcgca gcagctgacc tttcatcgcg tgccctacga cgtgccagcc gctgccgccg
     3181 ccatccgcgc caccccgctg cccgccttct ttgccgaccg gctggagttg ggccagtgaa
     3241 atcgctggtg ccgaacaccg tcatcgacgg tttcaagctg ggcgagatgc gccacaaggg
     3301 cggcatggcg ctgatctacg atgtggccta cgccgacggc cgcgccgcgc ccttccccat
     3361 ggtgatgaag gtgccgcgca tgacggccgg cgatggcgcc gagaccatcg tcagcttcga
     3421 ggtcgagcac cagatgatgc aggcgctgga cggcccgcac gtgccgcacc tggtggcggc
     3481 gggcgatttg tcggtgctgc cctatctggt gatggaatac gtgccgggcc gcacgctcga
     3541 cctttggctg gaccaggtca accagggcgt cctgctcgca cccgaaacgc tggcgcaact
     3601 gggcgccgcc gtggcgcacg ccgcgcacag cctgcacgaa cagaacgccg tacacctcga
     3661 cctgaagccg gccaacgtca tcatcaaggg cgacggcacg gcggtgctgc tggactttgg
     3721 cctcagcttt cacgcccact acccggattt gctggccgag gaaacgcgcc acgccgttgg
     3781 ctcgccagcc tacatgtcgc ccgagcaggt ggtgggtgtg cgcggcgacc cgcgcagcga
     3841 cgtattcgcc atcggcgtga tgctgtacga gctggcgacg ggcgagctgc cctttggcgc
     3901 gccgaccagc cagggcggcc tgcgccagcg cctgtggatg gatccgcaac cgccgcgcgc
     3961 gcgccggccc gacctgcccg agtggctgca agaggtcatc ctgcgctgcc tggagccggc
     4021 ggccgctgag cgctacccct cggctgcaca cctggccttc gacttgacgc accccgacca
     4081 ggtgcaggtc accgagcgcg gccagcgcct gaaaggcacc ggcgccggca cgcacatcaa
     4141 acgctggctg cgcgccgccg gggcagagta ccagccgagc ccgctgccgg cgcgccagat
     4201 tgccgaggtg ccgatcctga tggtggccgt gccgcaccgc gacgtgaccg acgccacgct
     4261 gtactcattg cgcgaggcgg tcggccgctc gctcggcatc cgccccggcg cgcggctggc
     4321 ggtggtcact gtggtggcgc ccggcgccgc cagcacaacc gacgccggca agagcgaaac
     4381 cgcgctgcac cgtcagatgc tggccatgct gaaaaactgg accgtgggcc tgaagctgga
     4441 cggccatcag gtgtcgtacc acgtgctgga agacggcgac ccggccgacg ccatcgtgcg
     4501 ctacgccgaa accaaccgcg tcggcatgat cgtgatgggc gccgccacgc acggcctgca
     4561 gttgcagcgc tttgtcgcca cggtgcccat caaggtcgcc atgcgcgcgc catgcacggt
     4621 gattctggtc aagcagacga tgccgtttgc ggagttgcat caggccggcg cgaagagcga
     4681 agtttgagcg ttttaggcct ccagcgcttt agtatcaagc gcgagaagct attttttgaa
     4741 tagcaacccg caaaggctat ccaaccccgt tcgtcctggg cctgtcgaag gacttggtgc
     4801 tatgagcgca ggcttcgaca agctcagccc gaacggatga gggccggatg tctgtcaata
     4861 cccgtgcgac cgcttcacca cgcccgctgg cagcgcgccc cgcatcagcg cccgcacgcc
     4921 aagcgccacc tgcaccaccg catcgcgccg caccgtctgc gcgctgatgt gcggcgtgac
     4981 gttgacgaag ggctgcgtcc acagccagtg atcgggcggc agcggctcgt cttcaaacac
     5041 atccagcgtc gcgccggcca gatggccttc ttccagcagg ttgcgcaggt catcttccac
     5101 caccagcgcg ccgcgcgcca cgttgatcaa gtagccgcca agctgcaccc gctccagcgc
     5161 ctcgcggttc aggatgccgc gcgtctcatc cgtcagcggc agcaggcaca ccagcacgcg
     5221 cgtggctttg aggaaggcgc tgagttcgtc cttgcccgca tagcagcgaa caccttcaag
     5281 atcgcgtcgc gtgcggctcc agcccagcac aggaaagtca aattgccgca ccgcctgcgc
     5341 cacgcgcgcg cccaaggcgc ccaggcccat gatgccgacg gggaaatcgt cgcgctccca
     5401 cgggcggcgc ggcttccaaa gttgttcttt ctgctgctgc gcgtaccagt cgaactcgcg
     5461 gtaaaaacgg atcagcgcat ggcacacgta ctcggccatc tgcaccgcca tgcccgcgtc
     5521 ttcgatgcgg acaatctgca gatcgggcgg cagcttgagc gccatcagct tgtcgacacc
     5581 agcgcccagg ttgaacaccg cgttcaactg cggctgctcg tcaaacaatt gctgcggcgg
     5641 gttccagacg atggcggcgt cgtaatcggc cggcacgccg ggttgccatt catccacctg
     5701 cgcctcgggc aaggcgcggc gcaagtcgtt cagccatgcc tcagacggcc ggccatcccg
     5761 gttgcacagc agcagcttca cgcggcgccc atgcccaggc gcagcagctc ggcgccttcg
     5821 gtcacctgat cgcctggcgc gtacagcact tcgtcgacga cgccgtcttg cggcgcggcg
     5881 atggtgtgct ccatcttcat ggcttccatc acggccagcg gctggccttt ggtgaccttg
     5941 tcgccggcct tgacggcaat cgacaccacc ttgccgggca tcggtgccgt caggcgaccg
     6001 ccttcgcccg cgccttcgac ggcgtgggcc agcacgtcca gctcggtgat ggtggtggcg
     6061 ccatcggtgc cgaacacatg caccacctcg ccgcgtggga aggtgtgcac cacctggcgt
     6121 tggccggcgt agcgcacgtc gatggcgccg tccggctggc gcgtgaagga gagcggtgcg
     6181 gattcgccat cgcccacctt caactgcagg ccgtcgtcca ggtagcgcag cttggcgacg
     6241 accggctggc cggcgtattc caggtcgatg cggcgcgagg ccacgccgtg cgagcggaaa
     6301 ccatcggtgc ggctgaaggg atcatgagac tcggcatcgc gctcggccag cacggtgcgc
     6361 gccacggcgg cggccacggc catgtccacg cccagcgggt cctggttaaa caggtgttca
     6421 gattcgcgct ggatcagtgc cgtgtccagc ttgccctggc tgaacgattc ggtggccagc
     6481 acgcggcgca ggaactgcac gttggtc
//
LOCUS       DAOMVO010000124         6500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3183_length_6500_cov_16.903336, whole genome shotgun sequence.
ACCESSION   DAOMVO010000124 DAOMVO010000000
VERSION     DAOMVO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3183_length_6500_cov_16.903336"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PKV30_07400"
     CDS             <1..968
                     /locus_tag="PKV30_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30073.1"
                     /translation="SFRSRIYALMAGIVALVLVLVLAGWWMSGRALSATDTAYTEGLK
                     LARAIDNARSAQVNFQRQVQEWKNVLIRGGDAALRDTHWKGFEQREQLMDKELDALQA
                     ALGGMTGMEGIASAAAKTRAEHGVLGQRYRDALQKYPTIDAAAQQAIDVSVRGMDRPT
                     SAGIDTLVQNLQGLVKQRFADESAAAHSPMRNNLLLVGGVAAALMALLLGAALAMARS
                     VFNSLGAEPEHAVRATARIAGGDLTERLNAKTPKSLIGALEMMQSRLRNISLAIREVA
                     FDIESRTTAMPQGPAREPLAQTWVGGHGWCAVEGVPGEKRRNWAS"
     gene            complement(1155..1955)
                     /locus_tag="PKV30_07405"
     CDS             complement(1155..1955)
                     /locus_tag="PKV30_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL30074.1"
                     /translation="MSRPLDDILFGAPSLRAQAVLRTMNWTAAAVLLVLAAGVALQFH
                     AAGQLDARFWEFFAWPTTWSFLAKGLLGTMASAAMAAVIALTLGLVLLVGRLARWRLL
                     RWPSVAVIEFLRGTPTLLLIYVCFLVLPQVGIKLSTYWMLTLPIALSTAAVVAEVYRA
                     GVLAVPRGQTEAARSLGMTGAEVFFSIVFPQALRYIVPALVAQLVIVVKDTTFGYVVT
                     YGELMQNAKVLIANYHSLVPVYLIVALLYCLVNYAISRASRWLGRPMH"
     gene            complement(1952..2614)
                     /locus_tag="PKV30_07410"
     CDS             complement(1952..2614)
                     /locus_tag="PKV30_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446388.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRL30075.1"
                     /translation="MGDVSRLLAEYGPAFGQALLLTWKLTVLSFVPGFLLGVLVTVLR
                     LLPLPPLRFVLTGYVEIFRNIPSVALLIFIVFALPDLQVVIDYEPSVILTLALVCSAF
                     TADYLRTGINTVAGGQAEAALSLGMRPLQVITAVVLPQALRSVVQPMTSLLIALMLST
                     SLASQVPFPGRELTTLVSKIANDSAAGMAAFAVAAALYVATGLLIGWAGAMLEKKLRI
                     LR"
     gene            complement(2657..3559)
                     /locus_tag="PKV30_07415"
     CDS             complement(2657..3559)
                     /locus_tag="PKV30_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL30076.1"
                     /translation="MSIKSVLKSAVVAWVTVASGSLFAQGTPINAAAYDALVAQGPVA
                     SAATVASSTWASKIKQAGTLRLGGTQTSNLFSLLNEKDGKMRGFDAGLAQLLARYILG
                     DGAKTQFTQVTSSTREQVLINDQVDLVLATYSITPARAEKISFAGPYYTSQAAVLVKG
                     SNKAIQSYNDLAGKKVATQAGSTGPAILAQHAPKAVVQEFQTHQEALDALRQGRVDAY
                     VTDHTLLLNALSLGAGDMKLAGAPFGAQDPYGIGLPKGSDGVAFVNAFLKKVEADGIW
                     AKLWTIAIGERTGSTAVPTPPVLP"
     gene            3705..4511
                     /gene="hutG"
                     /locus_tag="PKV30_07420"
     CDS             3705..4511
                     /gene="hutG"
                     /locus_tag="PKV30_07420"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HRL30077.1"
                     /translation="MTAPSPFTLHPGTAPLLISFPHVGTHVPPALAARLTPKAREVHD
                     TDWHLPRLYDFARGLGASLLIATHSRYVIDLNRPPDGASLYPGQSVTALCPVDDFDGE
                     SIHLDVADEPGAQEIAERRAAIWQPYHDALAAELARIKAAHGVAVLWDAHSIRSVLPR
                     FFDGKLPDLNLGTADGASCAPELAQALLAVAESAPGFTAVLNGRFKGGHITRAHGRPQ
                     DGVHAVQLEMTQSSYMQEAMPFDYLPEQAARIQPTLERLLQTALAFAQRA"
     gene            4623..5669
                     /locus_tag="PKV30_07425"
     CDS             4623..5669
                     /locus_tag="PKV30_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRL30078.1"
                     /translation="MKPLAYTRAAQLPDILRQRLVILDGAMGTMIQRFKLTEAQYRGE
                     RFKDFPKDVKGNNELLSLTRPDVISAIHEQYLAAGADIIETNTFGATQVAQADYDMAH
                     LAREMNVASARLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVNDPGARNVDFEA
                     LRASYYEQVEGLVEGGADVLLVETIFDTLNAKAALFAIDEFFEKSGERLPIMISGTVT
                     DGSGRVLSGQTVTAFWYSVRHAEPLSIGLNCALGAVMMRPHIQELNRVAPDVFISAYP
                     NAGLPDPMSETGFDEPPEVTCRLVREFAAEGLVNIVGGCCGTTPDHISAIAQAVNPLK
                     PRGVHTAGFYAEAA"
     gene            5666..>6500
                     /locus_tag="PKV30_07430"
     CDS             5666..>6500
                     /locus_tag="PKV30_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRL30079.1"
                     /translation="MRNFKPNRLLAHVLLAPAAYFLIANDAWAQFDPGREATEAPGVA
                     AHFPDPAGVRFATPGFDPAQPDFTSHAQMMARLQALAESHPRSVRLGSAGASAQGRDL
                     PTVLLAEGGRWRPERPTLMLVAHQHGNEPAPGEAMLVLLERWAAPESAALLKRVNVVA
                     LPRANPDGAAAFKRVGSDGTDINRDHLLLRTPEARAMAAVATRYRPQVVLDMHEFTAG
                     ERWIKKFGGWTSTDVMLQSAMTGNLNPEIAQAGLDVFLPAIRRAVQAEGLRDDWYNTT
                     SA"
BASE COUNT         1156 a         2212 c         2150 g          982 t
ORIGIN      
        1 tgagttttcg cagccgaatc tatgccttga tggccggtat tgtcgccttg gtgttggtgc
       61 tggtgctggc cggctggtgg atgtcgggcc gcgcgctcag cgccaccgac accgcttaca
      121 ccgagggcct gaagctcgcc cgggccatcg acaacgcgcg cagcgcgcag gtcaacttcc
      181 agcgccaggt gcaggagtgg aagaacgtgc tgatccgcgg cggcgatgca gccctgcgcg
      241 acacgcactg gaaaggcttc gagcagcgcg agcagctgat ggacaaggaa ctcgacgcgc
      301 tgcaagccgc gctgggcggc atgaccggca tggaaggcat cgccagcgcc gccgccaaga
      361 cgcgcgccga acacggcgtg ctgggccagc gctaccgcga cgcgctgcaa aaatacccga
      421 ccatcgatgc cgccgcgcag caggccattg acgtctcagt gcgcggcatg gaccggccca
      481 cgtcggccgg catcgacacg ctggtgcaaa acctgcaggg cctggtcaag cagcgctttg
      541 ccgatgaatc cgccgccgcg cacagcccca tgcgcaacaa cctgctgctg gtgggcggcg
      601 ttgccgcggc gctgatggcg ctgctgctgg gcgccgcgct ggccatggcg cgcagcgtgt
      661 tcaattcgct cggcgccgag cccgagcacg cggtgcgcgc caccgcccgc atcgccgggg
      721 gcgacctgac cgagcggctg aacgccaaga cgcccaagtc gctgatcggc gcgctggaga
      781 tgatgcaaag ccgcttgcgc aacatcagcc tggcgatccg cgaggtggcg ttcgacatcg
      841 aatcgcgcac caccgccatg ccgcagggcc cggcgcgcga gccgctggcg cagacgtggg
      901 tgggggggca cggctggtgc gcggtggaag gcgtgccggg agagaagagg cgcaactggg
      961 cttcctgaga gcgcgagaga agttgctggg agatcggtag ctcgatggag gcgagcctgg
     1021 atggatcacc tcgcgcggtg gacgtttccg tcggtcgtca catgcccgcc aactcccggc
     1081 cgccgccgcg atcgaatcca caccgcttcc cctcgccggg acgcatcacc ggcgcgcgaa
     1141 aaacgtggcg atggtcaatg catcggccgg ccgagccatc ggctcgcccg tgaaatcgcg
     1201 tagttcacca ggcaatacag cagcgcgacg atgaggtaca cgggcaccag cgagtggtag
     1261 ttggcaatga gcaccttggc gttttgcatc agttcgccgt aggtgacgac gtagccgaac
     1321 gtggtgtcct tcaccacgat gaccaattgc gcgaccagtg ccgggacgat gtagcgcagc
     1381 gcctgcggga agacgatgga gaaaaacacc tcggccccgg tcatccccag gctgcgcgcc
     1441 gcttcggtct ggccgcgggg cacggcgagc acgcccgcgc gatagacctc ggccacgacg
     1501 gcggcggtgc tcaaggcgat gggcagcgtc agcatccagt acgtgctcag cttgatgccg
     1561 acctgcggca gcaccagaaa gcacacatag atgagcagca gcgtcggagt gccgcgcagg
     1621 aactcaatga ccgccacgct cggccatcgc agcagtcgcc agcgcgccag gcgcccgacc
     1681 agcagcacca acccgagcgt caatgcgatg acggccgcca tcgcggccga ggccatggtt
     1741 ccgagcagtc ctttggccag aaaggaccag gtggtcggcc aggcgaagaa ttcccagaat
     1801 cgggcgtcga gctgacctgc ggcatggaac tgaagcgcga cgcccgccgc cagcaccagc
     1861 agcacggcgg ccgcggtcca gttcatcgtc cgcaggacgg cctgggcgcg caggcttggg
     1921 gcgccgaaca ggatgtcatc cagcgggcgg ctcatcgcag gatcctcagc ttcttctcca
     1981 gcatggcgcc cgcccagccg atgagcaaac ccgtcgccac gtacagtgcc gccgccaccg
     2041 cgaacgcggc catgccggcg gcggaatcgt tggcgatctt ggacaccaac gtggtgagtt
     2101 cgcggcccgg aaacggcacc tgcgatgcca gcgaggtcga cagcatcagc gcaatgagca
     2161 gcgaggtcat gggctgcacc accgagcgca gcgcctgcgg cagcaccacc gctgtgatga
     2221 cttgcagcgg ccgcatgccc agactgagcg cggcctctgc ctggccgccg gcgacggtgt
     2281 tgatgccggt gcgcaggtag tccgccgtga acgcggagca gaccagcgcc agggtcagga
     2341 tcacgctggg ctcatagtcg atcaccacct gaagatcggg cagcgcgaac acgatgaaga
     2401 tcagcaaggc cacgctggga atgttgcgga agatctcgac gtagccggtc agaacgaagc
     2461 gcagcggtgg cagcggcaac agccggagca ccgtcaccag aacgcccagc aggaagccag
     2521 gcacaaacga caggacggtg agcttccatg tcaacaacag ggcctgcccg aaggcaggcc
     2581 cgtattcggc caggagcctg gaaacatcgc ccacgggcca gcccagaaaa aacggcggaa
     2641 tcgaccgtgc gctggatcag gggagcacgg gcggcgtcgg caccgccgtg ctgccggtgc
     2701 gctcaccaat ggcaatcgtc cacagcttgg cccagatgcc atcggcttcc accttcttca
     2761 gaaaggcatt gacgaaggcc acgccgtccg agcccttggg caatccgatg ccgtacggat
     2821 cctgcgcacc aaagggcgcg cccgccagtt tcatgtcgcc ggccccgagg ctcaaggcat
     2881 tgagcagcag cgtgtggtcg gtcacatagg cgtccacgcg gccctggcgc agcgcgtcca
     2941 gcgcttcctg gtgcgtctgg aactcctgca ccaccgcctt gggcgcgtgc tgcgccagga
     3001 tcgctggccc ggtggagccc gcctgcgtgg cgaccttctt gccggccaga tcgttgtacg
     3061 actggatcgc cttgttgctg cccttcacca gcaccgcggc ctgcgaggtg tagtaggggc
     3121 cggcgaacga gattttctcg gcgcgggcgg gggtgatgga gtaggtggcc aacaccaggt
     3181 ccacctgatc gttgatgaga acctgttcgc gcgtggacga agtcacctgc gtgaactgcg
     3241 tcttggcccc atcacccagg atgtagcgcg ccaggagctg cgccaggccg gcgtcgaagc
     3301 cgcgcatctt gccgtccttc tcgttgagca gcgaaaagag attggaagtt tgcgtgccgc
     3361 ccaggcgcag cgtgccagcc tgcttgatct tgctggccca ggtgctcgat gcgacggtgg
     3421 cagcgctggc caccgggccc tgcgccacca gcgcgtcata cgctgccgcg ttgatggggg
     3481 tgccctgtgc aaaaagcgag ccactggcga cggtgaccca tgccaccacg gcggatttca
     3541 gaactgattt gattgacatg cggccctttc aaatgacgcg ttatacaaat caatatatat
     3601 cagctgcgac atgcgcgcac cgctcgttcg gtcagcccat cgacgcgcgg gccatgcggc
     3661 aagtgtaggc ttggccttgc gaccttcagc ttgtgagcaa aaccatgacc gccccttcgc
     3721 ccttcactct ccatcccggc accgccccgc tgctgatttc cttcccccac gtcggcaccc
     3781 acgtcccgcc cgcgctggcg gcgcggctga caccgaaggc gcgcgaggtg cacgacaccg
     3841 attggcacct gccgcgcctg tacgactttg cgcgcggctt gggcgcatcg ctgctcatcg
     3901 ccacgcattc gcgctacgtg atcgatttga accgcccgcc cgatggcgcc agcctgtacc
     3961 ccggccagag cgtgacagcg ctgtgcccgg tggacgattt cgatggcgag tcgattcatc
     4021 tggacgtggc cgacgagccc ggcgcgcagg agatcgccga acgccgcgcg gccatctggc
     4081 agccttacca cgatgcgctg gcggcggaac tcgcgcgcat caaggccgcg cacggcgtgg
     4141 cggtgctgtg ggacgcgcat tcgatccgct cggtgctgcc gcgttttttt gacggcaagc
     4201 tgcccgacct gaacctgggc acggccgatg gcgccagttg cgcgcccgag ctggcgcaag
     4261 cgttgctggc cgtggccgag tcggcgcccg gcttcacggc ggtgctcaac gggcgcttca
     4321 agggcgggca catcacgcgc gcgcatgggc gcccgcaaga cggcgtgcac gcggtgcagc
     4381 tggaaatgac gcaaagcagc tacatgcagg aggcgatgcc cttcgactac ctgcccgagc
     4441 aggccgcgcg cattcagccg acgctggagc gcttgctgca gacagcgctg gcgtttgcac
     4501 agcgtgcttg aaacctcgct tgacgctatt gtgttgatag ctgcttgcgc ttgccctcag
     4561 tgcgccagcg gccaattctt ctcatcaacg cagctgcggc agcggctttc tacaatgcag
     4621 gcatgaaacc gcttgcctac acccgcgccg ctcaactgcc cgacatcctg cgccaacgtt
     4681 tggtcattct ggatggcgcc atgggcacga tgatccagcg cttcaagctg accgaggcgc
     4741 agtatcgggg cgagcgcttc aaggactttc cgaaggacgt gaagggcaac aacgaactgc
     4801 tcagcctgac gcggcccgac gtcatcagcg ccattcatga gcagtacctg gccgccgggg
     4861 ccgacatcat cgagaccaac acctttggcg ccacgcaggt cgcgcaggcc gattacgaca
     4921 tggcgcacct ggcgcgcgag atgaacgtcg cctcggcccg gctggcgcgc gcggcgtgcg
     4981 acaagtacag cacgcccgac aagccacgct ttgtcgccgg tgccttgggc cccacgccca
     5041 agacggccag catcagcccc gacgtgaacg accccggcgc gcgcaatgtc gatttcgagg
     5101 cgctgcgcgc gtcgtattac gagcaggtcg aagggctggt cgaaggcggc gccgacgtgc
     5161 tgctggtcga gaccatcttc gacaccctca acgccaaggc cgcgctgttc gccatcgacg
     5221 aattcttcga gaaaagcggc gagcgcctgc cgatcatgat cagcggcacc gtcaccgacg
     5281 gctcgggccg cgtgctgagc ggccagaccg tcaccgcctt ctggtacagc gtaagacacg
     5341 cagagccgct gtcgatcggc ctcaactgcg ctttaggcgc ggtgatgatg cgcccgcaca
     5401 tccaggagct gaaccgcgtg gcgcccgacg tgttcatcag cgcctacccc aacgccggcc
     5461 tgcccgaccc gatgagcgag accggctttg acgagccgcc tgaagtcacc tgccggctgg
     5521 tgcgcgagtt tgcggccgag gggctggtca acatcgtcgg cggctgctgc ggcaccacgc
     5581 cggaccacat cagtgcgatt gcgcaggcgg tgaatccgct caagccgcgc ggcgtgcaca
     5641 cggcgggctt ctacgcggag gcggcatgag aaatttcaag ccaaatcgcc tgctggcgca
     5701 cgtcctgctt gcgccagcag cttatttttt gatagcaaac gacgcctggg cgcagttcga
     5761 ccctggccgt gaggcgaccg aagcgccggg cgtggccgcg catttccccg acccggctgg
     5821 cgtgcgcttt gccacgccgg gtttcgaccc cgcgcagccc gatttcacca gccacgcgca
     5881 gatgatggcg cgcctgcagg ccttggccga atcgcacccg cgcagcgtgc gcctgggcag
     5941 cgccggcgcc agcgcgcagg gccgcgattt gcccaccgtg ctgctcgccg aaggcggccg
     6001 ctggcggccc gagcgaccga cgctgatgct ggtcgcgcac cagcacggca acgagccggc
     6061 gccgggcgag gccatgctgg tgctgctcga acgctgggcc gcgcccgaaa gcgcggcgct
     6121 gctcaaacgc gtgaacgtcg tcgccctgcc gcgcgccaac cccgatggcg cggccgcctt
     6181 caagcgcgtg ggctctgacg gcaccgacat caaccgcgac catctgctgc tgcgcacacc
     6241 cgaagcgcgc gccatggccg ccgtcgccac gcgctaccgc ccgcaggtgg tgctcgacat
     6301 gcacgagttc accgccggcg agcgctggat caagaaattc ggcggctgga cctcgaccga
     6361 cgtgatgctg caatcggcca tgaccggcaa cctcaacccc gagatcgcgc aggccggact
     6421 ggatgtgttt ctgcccgcca ttcgacgcgc cgtgcaggcc gaggggctgc gcgacgactg
     6481 gtacaacacg acctcggccg
//
LOCUS       DAOMVO010000125         6498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3186_length_6498_cov_12.432873, whole genome shotgun sequence.
ACCESSION   DAOMVO010000125 DAOMVO010000000
VERSION     DAOMVO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_6498_cov_12.432873"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..956)
                     /locus_tag="PKV30_07435"
     CDS             complement(66..956)
                     /locus_tag="PKV30_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30080.1"
                     /translation="MCLLERRIQRDLRHAGFADSELRAVRESPPMWAQLTAFYRADGR
                     IERVEAAFSGATGQPGLIRFFSPPAPAVCTHASYGALAHELGHALQFPPQWQPPGAFD
                     SAPAYARARELGEAHAWLNQYRLVRAKQGGAPETQQLQLIENDADFATQPVDLFARIA
                     EREAAGWSDAQILDELAVLNANMFPCGMGEGNFKTYGQCNRWDWWQATQARHPAFRDF
                     LQRLPRAPNANDQKVLMKFNLLSAPASADAQALARWADALGGCGPQGGLDALYALSCA
                     ALPGARPGVGAGAIQSSKSA"
     gene            994..1386
                     /locus_tag="PKV30_07440"
     CDS             994..1386
                     /locus_tag="PKV30_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30081.1"
                     /translation="MNRLTADDLRQLQTHAPAAPACRCGVGACDGWVSLSPERWPMAQ
                     MQAIGTLRDMAVHEPSFEELHPHGTRYESPAAPVAPHFYPYNRCDLYRCTGCQRLLLR
                     YTEAGGYYVDERVRVLAPGLPVLDDAVS"
     gene            1632..2534
                     /locus_tag="PKV30_07445"
     CDS             1632..2534
                     /locus_tag="PKV30_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL30082.1"
                     /translation="MPLSHILLGLAVVAVWGTNFVVIKYALAHLPPLALATLRFALAA
                     FPALLFIRRPAASWRNLAAYGLLIGVAQFGLLFIAMTRFISPGLASLVVQVQVFFTIG
                     MAMLLAGERVRVFQLLAMALSVAGIALIGWHAVTDGAAITPLGLVLILLAALAWACGN
                     IVSRAAGRVDALGYMVWSSLFAVPPLVALSLALEGWPAVRAGLVAADWVTWAAVLWQS
                     VGNTLFGYGAWAWLLARHPAASVTPLALLVPVFGMGASALWLAEPLPGWKISAALLVL
                     GGLALNLLWPRLRGWIVASERPAR"
     gene            complement(2552..2917)
                     /locus_tag="PKV30_07450"
     CDS             complement(2552..2917)
                     /locus_tag="PKV30_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30083.1"
                     /translation="MAGPIGTAVGAVIGAVVGGLAGKSVDETVDPTAEEAYWRESYTR
                     EPYYRDGHTYDDYAPAYGLGIGSLVADKYRERSFDDIEGSLAEEWDMYRGNSRLNWTE
                     ASPAARAAWARAQRDRGAV"
     gene            complement(3174..3614)
                     /locus_tag="PKV30_07455"
     CDS             complement(3174..3614)
                     /locus_tag="PKV30_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30084.1"
                     /translation="MNTFTADPSHFTMGGAYYPSGHVFAMFTDEAAAQAAAAQIAAVA
                     GVGAITLATPAAIQQAFAQRAADVGTGAPSVGREDQFMLRFVELAQAGKAGLLIELAD
                     AEVEAISAILNASGALLAYCYRKLVIEELVDASPRAEAAAAGKL"
     gene            3814..4398
                     /locus_tag="PKV30_07460"
     CDS             3814..4398
                     /locus_tag="PKV30_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30085.1"
                     /translation="MAALIAAAGAGAHAQVVPVYENDFESPNVTSCDASSFQSSGFTN
                     SYNGTGVPPATFQQVNTADRLCVAQPVAAGPYYDPAGTAGRFTCGFVRHNDDNWVESC
                     ATTFDPKGQAFLNVSIDWAMVQLLAQKQFDPTAAQDVVLRFFCVPAGQTMAITDAGGG
                     APANVSGVAPPTALQTSPFTVQPPPNQATNPKAI"
     gene            4423..4644
                     /locus_tag="PKV30_07465"
     CDS             4423..4644
                     /locus_tag="PKV30_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30086.1"
                     /translation="MTVPLSTCPAGSQVGMVAHIAGGNTDMAFDNLRVTASATPGGVA
                     PAAVPINAPLALLGAVLGLGWLGARRLRP"
     gene            complement(4786..5781)
                     /gene="dusA"
                     /locus_tag="PKV30_07470"
     CDS             complement(4786..5781)
                     /gene="dusA"
                     /locus_tag="PKV30_07470"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HRL30087.1"
                     /translation="MSAMSPWRLSVAPMLDWSDKHGRYLHRLITKKTRLYTEMVTTGG
                     LLHGGERRHLRYDEVEHPLALQLGGSEPADLARCAQIGARWGYDEINLNCGCPSERVQ
                     RGAFGACLMAEPQLVADGVKAMVDAVDVPVTVKHRIGIDKEESYAFVRDFVGTVADAG
                     CRVFIVHARNAWLKGLSPKENREIPPLRYELVYQLKRDFPHLTIVLNGGVTTDEQIAE
                     HLQHVDGVMLGREAYHHPWMMTAWDARFLGGAPNPLTPDEVEAAMVAYMQRELVEDGT
                     PWSAIARHMLGLHHGRRGARLWRQVWSDHKLKPLPAPEVAALARAHLASAAMAVA"
     gene            complement(5778..6191)
                     /locus_tag="PKV30_07475"
     CDS             complement(5778..6191)
                     /locus_tag="PKV30_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL30088.1"
                     /translation="MLVLDSNTISYYFRGDAQVVPRMQALPPADLGVPAIVEYELRYG
                     LLRLAPEAAEPRLAALAQLLRPMQLLPFDSECAAHAARIRAALEAAGTPIGPHDTLIA
                     ATALRHQATLVTRNVSEFGRVANLQCLNWHAEGVA"
     gene            complement(6207..6437)
                     /locus_tag="PKV30_07480"
     CDS             complement(6207..6437)
                     /locus_tag="PKV30_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRL30089.1"
                     /translation="MSQITLYLDDATQALVEQAALANGVSKSRWVADIIRKHAGHEWP
                     RECLAMAGRFADFPLREEPVAAGVADVARVGF"
BASE COUNT          967 a         2183 c         2221 g         1127 t
ORIGIN      
        1 cggcatcatg tcactctatt tttgatagct gctcgcgctg gatggacaag cgccaacggc
       61 cgttttcatg cagattttga tgactgaatc gcgcccgcgc ccacgcccgg ccgggcccca
      121 ggcaaggccg cgcagctcag cgcgtacaac gcatccagcc caccttgcgg accgcacccg
      181 cccagcgcat cggcccagcg cgccagggct tgggcatcgg cgctggccgg tgccgacagc
      241 aggttgaatt tcatcaacac cttctggtca ttggcgttgg gcgcgcgcgg caggcgctgc
      301 agaaaatcgc ggaacgccgg gtggcgcgcc tgcgtggcct gccaccagtc ccagcggttg
      361 cactggccgt aggtcttgaa gttgccctcg cccatgccgc agggaaacat gttggcgttg
      421 agcacggcca gttcatccag aatttgcgcg tcgctccagc cggccgcttc gcgctcggcg
      481 atgcgggcaa acagatcgac cggctgggtg gcaaagtcgg cgtcgttctc gatcaattgc
      541 agttgctgcg tctccggcgc gccgccctgc ttggcgcgca ccagccggta ttggttgagc
      601 caggcgtgcg cctcgcccag ctcgcgcgcg cgggcatagg ctggcgcgct gtcgaaggcg
      661 ccgggcggtt gccactgcgg cggaaattgc agcgcatggc ccagctcgtg cgccagcgcg
      721 ccgtagctgg cgtgcgtgca gacggccggc gcgggcgggc tgaaaaagcg gatcaggcca
      781 ggctgcccgg tggcgcccga gaacgccgcc tcgacgcgtt cgatgcgccc gtcggcgcgg
      841 taaaaggccg tgagctgcgc ccacatcggc ggcgattcgc gcaccgcacg caactcgctg
      901 tccgcgaagc ctgcgtggcg caggtcgcgc tggatgcggc gttcaagcaa gcacatggtg
      961 cgcccaactg tagcgcggcc ccacaatgcc ggcatgaacc gcctcaccgc cgacgacctg
     1021 cgccagttgc agacgcatgc gcccgccgcg cccgcttgcc gctgcggcgt gggcgcctgc
     1081 gacggctggg tcagcctgtc gccagagcgc tggccgatgg cgcagatgca agccatcggc
     1141 acgctgcgcg acatggccgt gcacgagccg agctttgaag agctgcaccc gcacggcacg
     1201 cgctacgaat cgccggccgc accggtggcg ccgcacttct acccctacaa ccgctgcgac
     1261 ctgtaccgct gcacggggtg ccagcgcctg ctgctgcgct acaccgaagc cggcggttat
     1321 tacgttgacg aacgcgtgcg cgtgttggcg cccgggctgc cggtgctgga cgatgcagta
     1381 tcttgattgc ttcataattc atagcttctc acgcttgatg gtcaagcgct ggcggggttt
     1441 ttgatgaaga aatgaccgca cgctccatgg cgcgcagcgc cgtcatcgaa ggcgccagcc
     1501 gaggtcatgc gccgcaggcg cgtcatcaaa aaccatagct gctcgcgctt gccagacaag
     1561 cgcgagaggc cgatttcttt taaaaccacc gtcacatgaa cgcttccctg cccaagccgc
     1621 cgtccggcgc gctgcccctg tcccacatac tgctggggct ggcggtggtg gcggtgtggg
     1681 gcaccaattt cgtcgtcatc aagtacgcgc tggcgcattt gccgccgctg gcgctggcca
     1741 cgctgcgctt tgcgctggcg gcgtttccgg cgctgctgtt cattcgccgg ccggcggcca
     1801 gctggcgcaa tctggcggcg tatgggctgc tgatcggggt ggcgcagttc gggctgctgt
     1861 tcatcgccat gacgcgcttc atctcgcccg gcctggcgtc gctggtggtg caggtgcagg
     1921 tgttcttcac catcggcatg gcgatgctgc tggcgggtga gcgggtgcgg gtgtttcagc
     1981 tgctggcgat ggcgctgtcg gtcgccggca tcgccttgat cggctggcac gcggtgaccg
     2041 atggcgcggc catcacgccg ctcgggctgg tgctgattct gctggcggcg ctggcctggg
     2101 cctgcggcaa catcgtcagc cgcgccgccg ggcgggtcga tgcgctgggc tacatggtgt
     2161 ggtcgagcct gttcgctgtg ccgccgctgg tggcgctgtc gctggcgctg gaaggttggc
     2221 ccgccgtgcg cgccggcttg gtcgccgccg attgggtgac gtgggcggcg gtgctgtggc
     2281 agtcggtcgg caacactttg ttcggctacg gggcctgggc ttggctgctg gcgcgccacc
     2341 ccgccgccag cgtgacgccg ctggcgctgc tggtgccggt gttcggcatg ggcgcctcgg
     2401 ccctgtggct ggccgagccg ttgccgggct ggaagatcag cgccgcactg ctggtgctgg
     2461 gcgggctggc cctgaacctg ctttggccgc gtctgcgcgg ctggatcgtc gcgtcggaac
     2521 ggcccgcgcg ctgagcgcag gccgagcgcg tttacactgc gccgcgatcg cgctgcgcgc
     2581 gcgcccaggc ggcacgcgcg gcggggctgg cttcggtcca gttgaggcgc gaattgccgc
     2641 ggtacatgtc ccattcctcg gccagcgagc cttcaatgtc gtcaaaactg cgttcgcggt
     2701 acttgtcggc caccaggctg ccgatgccga ggccgtaggc cggcgcgtag tcgtcgtagg
     2761 tgtggccgtc gcggtagtag ggctcgcgcg tgtagctctc acgccagtag gcctcttcgg
     2821 ccgtcgggtc aaccgtttcg tccaccgact tgccggccag cccgccgacc acggcgccaa
     2881 tcaccgctcc gacggcggtg ccgatgggcc cggccatcga gccgacggcc gcgccccgta
     2941 gcgccgcccg ccgccgcgcc cacgccggtg cccaccgggt gcgcgcccgg cgcgccggtg
     3001 atggggtcga agttgagatc cttgtcggtg ctgctcatgt ctggtctcct tcgacaaagc
     3061 gggatgcccg cgccgccgac ggcagcgcat gctttcaggg tagaagtggc gacagatgag
     3121 gctgtaggat ggccgcgcgc gccagcgcgg ggcgatgcgg ccagcgcctg cttttacagc
     3181 ttgccggctg cggcggcttc ggcgcgcggg ctggcgtcga ccagttcctc gatcaccagt
     3241 ttgcgatagc aatacgccag cagcgcgcca ctggcgttca ggatggcgct gatggcctcg
     3301 acctccgcgt cggccaactc gatcagcaaa cccgccttgc cggcctgcgc cagttcaacg
     3361 aaacgcagca tgaactggtc ctcgcgcccg accgatggcg cgccggtgcc cacatcggcc
     3421 gcgcgctggg caaacgcctg ctggatggcg gccggcgtgg ccagcgtgat ggcccccacg
     3481 cccgccaccg ccgcaatttg cgccgccgca gcctgtgccg ctgcctcatc ggtgaacatg
     3541 gcgaacacgt ggccgctcgg gtaataggcg ccacccatgg tgaagtggct ggggtcggcg
     3601 gtgaaggtgt tcatggcgtg tctttcgcag gctgtggttt gggatgaatt cagcttcgat
     3661 tgttctagca ccccacgccc gcgggtgtag gaaagcggcg gcttttgcag cggaaggcag
     3721 ccgacgtggc tgctgccgaa gactgcctag aatgttctgg aatcatgtga acaggtcagg
     3781 gagaaacgca tgaatcgacg ttgcgggcat ctcttggcgg cgctcattgc cgccgcgggc
     3841 gctggcgcac acgcacaggt ggtgccggtc tatgagaacg actttgaaag ccccaacgtc
     3901 acgagctgcg acgccagcag ctttcaaagc agcggcttca ccaacagcta caacggcacc
     3961 ggcgttccac cggccacttt ccagcaagtg aacacggccg accgcctctg cgtggcacag
     4021 ccggtcgccg ccggacctta ctatgacccg gcaggcacgg cgggcaggtt cacctgcggc
     4081 ttcgtgcgtc acaacgacga caactgggtc gagtcttgcg ccaccacctt cgatccgaaa
     4141 ggtcaggcct tcttgaacgt cagcatcgac tgggccatgg tgcagttgtt ggcgcagaag
     4201 cagtttgacc ccaccgccgc gcaggatgtg gtgctgcgct tcttctgcgt accggcgggc
     4261 cagacgatgg ccatcaccga tgcgggcggc ggtgccccag ccaatgtgtc cggggttgcc
     4321 cctccaacgg ccctgcagac gagcccgttc actgtgcaac cgccgcccaa tcaggccacc
     4381 aaccccaaag cgatttaagc cttctactgg cagacacagc gggtgacggt gccgctgtcg
     4441 acctgccccg ccggcagcca ggtcggcatg gtggcccaca tcgccggcgg caacaccgac
     4501 atggctttcg acaacctgcg cgtcacggct tctgccacgc ccggcggcgt ggcgccggct
     4561 gcggtgccga tcaatgcgcc actggccttg ctgggcgctg tactgggcct cgggtggttg
     4621 ggcgcgcggc gactgcggcc ctgaagtcgc gcgtgcgctg gcgcgcgcgt ctgctcacgc
     4681 aggcgcggcc ttcgttcgct atcgcgcaaa tagcaaagaa tgaggccaga cttgcgctgt
     4741 gcgcctgaaa cgtcctgaac gaacgcttcc aaaccggctc gacgcttacg ccacagccat
     4801 ggccgccgac gccagatgcg cgcgcgccaa cgccgccacc tcgggcgccg gcaagggctt
     4861 gagcttgtgg tcgctccaca cctggcgcca caggcgcgcg ccgcgccggc cgtggtgcag
     4921 gccaagcatg tggcgcgcaa tggccgacca gggcgtgccg tcttcaacca gttcgcgctg
     4981 catgtaggcg accatggccg cctcgacctc atccggggtg agggggttgg gcgcgccacc
     5041 cagaaagcgc gcgtcccacg ccgtcatcat ccatgggtgg tggtaagcct cgcggcccag
     5101 catcacgccg tcgacgtgct gcaggtgctc ggcaatctgc tcgtcggtgg tcacgccgcc
     5161 attcagcacg atggtcaggt gcggaaagtc gcgcttgagc tggtagacca gttcgtagcg
     5221 cagcggcggg atttcgcggt tctctttggg gctcaagccc ttgagccacg cattgcgcgc
     5281 gtgcacgatg aacacgcggc aacccgcgtc ggccactgtg ccgacgaagt cgcgcacgaa
     5341 ggcgtagctc tcttccttgt cgatgccgat gcggtgcttc acggtcaccg gcacgtcgac
     5401 cgcatcgacc atcgccttga cgccatcggc cacgagttgc ggctcggcca tcaggcaggc
     5461 gccaaacgcg ccgcgttgca cccgctcgct cgggcagccg cagttcaggt tgatctcgtc
     5521 gtagccccag cgcgcgccaa tctgcgcgca acgcgccaga tcggccggct cgctgccgcc
     5581 cagttgcagg gcgagtggat gctcgacctc gtcataacgc aagtgccgcc gttcgccgcc
     5641 gtgcagcaag ccgccggtgg tgaccatctc ggtgtacagg cgcgtctttt tcgtgatcag
     5701 ccggtgcagg tagcggccgt gcttgtcact ccaatcgagc atcggcgcaa cggacaggcg
     5761 ccagggggac atggcgctca tgccaccccc tcagcgtgcc agttcaggca ctgaaggttg
     5821 gcaacgcgcc cgaattcgct cacgttgcgt gtaaccaatg tcgcttggtg gcgcaaggcg
     5881 gtggcggcga tcagcgtgtc gtgcgggccg atgggtgtgc cggcggcctc cagtgcggcg
     5941 cggatgcggg cggcgtgggc cgcgcattcg ctgtcaaacg gcagcaattg catcggtcgc
     6001 agcaactgag ccagcgccgc caggcggggc tcggccgcct cgggcgccaa gcgcagcagg
     6061 ccgtagcgca attcgtactc aacgatggcg ggcacgccca gatcggcggg cggcagtgcc
     6121 tgcatgcgtg gcaccacctg cgcgtcgccg cgaaagtagt agctgatggt gttgctgtcg
     6181 agcacgagca tggcggcgat gttggtttaa aagcccaccc gggccacgtc ggcgacgccg
     6241 gccgccacgg gctcctcgcg cagaggaaag tcggcaaagc ggcccgccat ggccaggcac
     6301 tctcgcggcc attcgtggcc ggcgtgcttg cggatgatgt cggccaccca gcggcttttc
     6361 gagacaccat tggcgagggc agcctgttcc accagcgcct gcgtggcgtc gtccaggtac
     6421 agcgtgattt gggacatgcg gcgttccttc cggttctcgc gaacacttat atgtccaatt
     6481 atatgagtca aaagcagg
//
LOCUS       DAOMVO010000126         6460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3208_length_6460_cov_17.743013, whole genome shotgun sequence.
ACCESSION   DAOMVO010000126 DAOMVO010000000
VERSION     DAOMVO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3208_length_6460_cov_17.743013"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..2476)
                     /locus_tag="PKV30_07485"
     CDS             complement(269..2476)
                     /locus_tag="PKV30_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HRL30090.1"
                     /translation="MRADTLTDTFTALRELPRAVLHSVAPAVPRPVRHALRSGQRAMQ
                     LSQRVAWYSLLSALVLLAPPSRAQSADAENTKAESPYFFVKGAQPGVDALPLKSTDVQ
                     VNISGVIADVVVTQRYKNEGTVPIEARYIFPGSTKAAVNGLNVRIADRLVVAQIREKQ
                     QAKVEYDQAKKEGKTAALLEQHRPNVFQMNVANILPGEDVAVELRYNELLVPTDGNYQ
                     FVFPTVVGPRYAGTTSKAPSETGSGQTTAHAQAFPAQPVLRAGEVSPAAFNMKVNLAS
                     PIGIQEIRSPSHAIDTQMDAGSAAKRANVQLAANASRVANNRDFILDYRLAGSAIQSG
                     VLLHKGEKENFFLAMVQPPTAVPQAAITPRDYIFVVDISGSMHGFPLDTAKALMRQLL
                     GNLKASDTFNVLLFSGSNRFLAPNSVPATPANINAAIRTIEEMGGGGSTELLPALRRV
                     YTQPKSQELSRTVVVVTDGYVSVESEAFALVRKNLSQANVFAFGIGSSVNRHLMEGLA
                     RAGMGEPFIITKPSEAKEQAERFRKLIESPVLTSVKAKFEGLDVYDVEPQALPDVLAE
                     RPVIVFGKWREKTGSGPTRVVIEGRAANGPYTQSLPIDAQAEWQGTGALRHLWARHRI
                     AALSDEESLTGGDAQKAAITKLGLDYSLLTQYTSFIAVDKVVRNLGGQGATANQPSPL
                     PEGVSNLAVGEASALGAAVSSTPEPETWAAMLVVLAVLGAQVARRQRRQPFTA"
     gene            2728..4152
                     /gene="creD"
                     /locus_tag="PKV30_07490"
     CDS             2728..4152
                     /gene="creD"
                     /locus_tag="PKV30_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRL30091.1"
                     /translation="MNRLAAWRDSMLVKSLMLLLLLGVLCIPLWQIENLIDARGDSQQ
                     AARDELALTHVGPQKLIGPLMVVPYVESWQVAQRNERGEQIGLEPKSELRWQLQCPQQ
                     LDVNGSLVPEERYRGIFKVPFYKLSAKLGGHFAPLKPDQLPRRVKGSSIELLPPVLAL
                     ALSDPRGLEGAPKLALNGEALAFARGVPHVPEKSWLAAGVHAPLSAAAAQALVGSQPI
                     PFNLDVTLNGQTQLAIAPLGDETSAHFTSPWAHPRFGGRFLAAERTVTDSGFDARWRI
                     TALTTQAREQVRSSLHGEHARASLAGVEGLDTFDVALVQPVNVYSMSERAVKYGVLFI
                     GLVLMAVFMVELFKRLALHPVQYALVGLSIAVFFLLLIALSEKIGFALAYAGAAGASV
                     ALLAMYFSAVLGSVQRGGSLAAYVALLYGALYGLLASESNALLLGALLVFGMLAALMG
                     ATRHVNWWRLGGSDTGPAESPTPA"
     gene            4431..4676
                     /locus_tag="PKV30_07495"
     CDS             4431..4676
                     /locus_tag="PKV30_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30092.1"
                     /translation="MGAKKVRVEFVDGSGQGVGGLNVKATGCGELQTAPTGQAFFLVD
                     EENFAITVNGAEVYKGTLSNLPEKIVFKQDGGSWKAA"
     gene            complement(4758..6260)
                     /gene="creC"
                     /locus_tag="PKV30_07500"
     CDS             complement(4758..6260)
                     /gene="creC"
                     /locus_tag="PKV30_07500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HRL30093.1"
                     /translation="MSRRTRILLALIAVYAIGMAALLYQLLADLDPRYRESAEEGLIE
                     TAQLMATVIEQDVEYGALPTDRIGGIFRDLYARRFSAQIYNLHKTRVELRAYVTNRHG
                     QVVYDSTGRDLGKDYSGWRDVRNTLLGEYGARTTRDVAADKHSSVMYVGAPIRWNGEI
                     VGVVTLGKPVQSFGQFVSAARGNIISVGIVSALSALALALVLSFWLIRPLGITSDLLA
                     SLRTAWWHDEQGRRRFSPRRAWRALRAQARAAGQEVRDAIAGRNYVQDYVQQLTHELK
                     SPISAVRGAAELMQEPGMPPDQQQKFAANIARESHRMQEVVDRMMELTALESRRMLQD
                     QQRVELAPVLHELVAGARARAPRLQIVLQIQDELAVEGDAFLLRRAIGNLLDNAVEFS
                     TPAGDGSADIALTLARDGRMACITVRDHGPGVPPYAEGKVFDKFFSLARPGSPKRSTG
                     LGLSFVQEIATLHQGSVSLRNVQPGGEPVAVVERAARGARAVLRLPALKA"
BASE COUNT         1021 a         2077 c         2229 g         1133 t
ORIGIN      
        1 ggcggggcga gcgggtgagc aaggaggtgt tcatgatggt ttgggcctta agcgcctatt
       61 tggcaagcgc cagcagctat gactacggga gcattcgagt cacccggact cgctccctcg
      121 cccctctggg gagagggctg gggtgagggg cggcggcgtt ggatcaggtc gcgcggtcgg
      181 cccccagccc aaccctcccc cagcggagga gggagcaaga caccctcaca cctgccctct
      241 cccgctcgcg ggagagggag tctgagcatc acgcggtgaa cggctgccgg cgctggcgcc
      301 gcgccacttg cgcgcccagc acggccagca ccaccagcat cgcggcccag gtttcgggtt
      361 cgggcgtgct gctcaccgcc gcgcccagcg cgctggcttc gcccaccgcg aggttgctca
      421 cgccttcggg cagcgggctg ggctggttgg ccgtggcgcc ctggccgccg aggttgcgca
      481 ccactttgtc caccgcgatg aagctggtgt attgcgtgag caggctgtag tccaggccga
      541 gcttggtgat ggcggctttc tgcgcgtcgc cgccggtgag cgactcttca tccgagagcg
      601 ctgcgatgcg gtggcgtgcc cacaggtggc gcagggcacc ggtgccttgc cattcggctt
      661 gtgcgtcgat gggcagcgac tgcgtgtagg ggccgttagc agcgcggcct tcgataacga
      721 cgcgcgtcgg gccactgccc gtcttctcgc gccacttgcc gaacacgatc accggtcgct
      781 cggccagcac gtcgggcagg gcttgcggtt cgacgtcgta tacatccagg ccttcgaact
      841 tggccttcac gctggtcagc acgggcgatt cgatcagctt gcgaaagcgc tcggcctgct
      901 ctttcgcctc agagggtttg gtgatgatga acggctcgcc catgccggcg cgcgccaggc
      961 cttccatcag gtgccggttg accgacgagc cgatgccgaa ggcgaacacg ttggcttggc
     1021 tcaagttctt gcgcaccagg gcgaaggctt cgctttccac cgagacgtag ccatcggtca
     1081 cgacgacgac ggtgcgcgac agttcttgcg acttcggttg cgtgtacacg cggcgcagcg
     1141 cgggcagcag ttcggtcgat ccgcccccac ccatttcttc aatcgtgcgg atcgccgcat
     1201 tgatgttcgc cggcgtggca ggcacggaat tgggcgccag aaagcgattc gagcccgaga
     1261 acagcagcac gttgaaggtg tcgctggcct tcaggttgcc cagcaactgg cgcatcaacg
     1321 ccttggccgt atcgagcgga aagccgtgca tgctgccgga gatgtcgacc acgaagatgt
     1381 agtcgcgcgg cgtgatggcc gcctgcggca cggccgtggg tggctgcacc atggcgagga
     1441 agaagttctc tttttcgccc ttgtgcagca gcacgccgga ctggatcgcg ctgccagcca
     1501 gccggtagtc gaggatgaag tcgcggttgt tggcgacgcg gctggcattg gcggccagtt
     1561 gcacgttggc gcgcttggcg gcgctgccgg cgtccatctg cgtgtcgatg gcgtggcttg
     1621 gcgagcgaat ctcttgaatg ccgatggggc tggccagatt caccttcatg ttgaaggcgg
     1681 cggggctgac ctcgccggcg cgcagcacgg gttgagcggg gaaggcctgc gcgtgggcgg
     1741 tggtttggcc gctgccggtt tcgctcggcg ccttcgatgt ggtgccggca taacgcggtc
     1801 cgaccaccgt tggaaatacg aactggtaat tgccatcggt cggcaccagc agctcgttgt
     1861 agcgcaactc caccgccacg tcttcgccgg gcaggatgtt ggcgacgttc atctggaaca
     1921 cgttgggccg gtgctgctcc agcagggcgg cggtcttgcc ctccttcttg gcctggtcgt
     1981 attcgacctt ggcttgctgc ttttcgcgga tctgcgcgac gaccaggcgg tcggcaatgc
     2041 gcacgttcag gccgttcacc gccgccttgg tcgagccggg gaagatgtag cgcgcctcga
     2101 tcggcaccgt gccctcgttt ttgtagcgct gcgtgaccac cacgtcggca atcacgccgc
     2161 tgatgttgac ctgcacgtcg gtcgatttga gcggcagcgc atcgacgccg ggctgcgcgc
     2221 ccttgacgaa gaagtagggg ctctcggcct tggtgttctc ggcatcagcc gattgcgcgc
     2281 ggctgggcgg cgccagcagt accagcgccg acagcagcga ataccaggcc acacgctggc
     2341 tcagttgcat ggcgcgctgg ccgctgcgca gcgcgtggcg caccgggcgc ggcacggcgg
     2401 gtgccacgct gtgcagcacg gcgcgcggca gctcgcgcag cgcggtgaat gtgtcggtca
     2461 aggtgtcggc tcgcatggtc ttgttccttc tggggtgaag tgaggcgttg tgcgtggcgc
     2521 gccgccgtcc tcacatcgcc ggcagcgcat gaccgtgact gtgaaattcg cgctcgaagg
     2581 ggcgatgaag cgcgcaagaa agacgcacgc gctgtgtgaa gtcggcgtga agtgggggtg
     2641 aaacgcgggc tgcgcgcgct tcacacggtg ttcgcttgcg gcggcgagca tcggcgccgt
     2701 ttctcatcac acccttggag gaaaaccatg aaccgactcg ccgcctggcg cgattccatg
     2761 ctcgtcaaga gcctgatgct gttgctgctg ctcggggtgc tgtgcattcc gctgtggcag
     2821 atcgaaaacc tgatcgacgc gcgcggcgac agccagcagg ccgcccgcga cgagctggcg
     2881 ctgacccacg tcggcccgca gaagctgatc ggcccgctaa tggtggtgcc ctatgtcgaa
     2941 agctggcagg tggcgcagcg caatgagcgt ggcgaacaaa tcgggttgga gcccaaatca
     3001 gaactgcgct ggcagcttca gtgtccgcag caactcgatg tgaacggcag cctggtgccg
     3061 gaggagcgct accgcggcat cttcaaggtg ccgttctaca aactgtcggc caagttgggc
     3121 ggccactttg cgccactcaa gcccgatcaa ctgccgcgcc gcgtgaaggg ctccagcatc
     3181 gaactgctgc cgcccgtgct ggcgctggcg ctcagcgacc cgcgcgggct ggagggcgcg
     3241 cccaagctgg ccttgaacgg cgaggcgctg gcgtttgcgc gcggcgtgcc gcacgtgccc
     3301 gagaagagct ggctggccgc cggcgtgcat gcgccgttgt cggccgccgc cgcgcaggcg
     3361 ctggtgggca gccagccgat acccttcaac ctggacgtga ctctgaacgg ccagacccaa
     3421 ctggccattg cgccgctggg cgacgaaacc agcgcgcact tcacctcgcc ctgggcgcac
     3481 ccgcgctttg gcgggcggtt tctggcggcc gagcgcacgg tgaccgacag cggctttgac
     3541 gcacgctggc gcatcaccgc gctgaccacg caggcgcgcg agcaggtgcg ctcgtcgctg
     3601 cacggcgagc acgcgcgcgc gtcactcgcg ggtgttgaag ggctggacac cttcgacgtt
     3661 gcgctggtgc agcccgtcaa cgtctattca atgtccgagc gtgcggtgaa gtacggcgtg
     3721 ctgttcatcg gcctggtgct gatggcggtg ttcatggtcg agctgttcaa gcgcctggcg
     3781 ctgcacccgg tgcagtacgc gctggtcggc ttgtcgattg cggtgttctt tctgctcttg
     3841 atcgcgctgt cagagaagat cggcttcgcg ctggcctacg cgggcgcggc cggcgccagc
     3901 gtggcgctgc tggcgatgta cttcagcgcc gtgctgggca gcgtgcagcg cggcggctcg
     3961 ctggccgcgt acgtggcgct gctgtacggc gcgctgtatg ggctgctggc atcggaaagc
     4021 aatgcgctgc tgctgggcgc cctgctggtg ttcggcatgc tggcagcgct gatgggggcg
     4081 acgcggcatg tcaactggtg gcggctgggt ggttcagata cggggcccgc cgagtccccg
     4141 acgccggcct gattcgtcaa agccagctat aaaaaaccta gctgcccgcg cttgttggtc
     4201 ctgcgcaggc aacatgttta atcacatgtt aatcaggtga gtctttgatt catgtgcgtg
     4261 tgaaaaaagt cgtagcgggc gacgcacgtc cgctcgcaac cgagcaagtc aagcgtgcaa
     4321 tttgtcgcgc gagccgggtc gaacggtgtt caaaacgcct gcggcaatgg caaattcgag
     4381 gctcttttca accaacgctg agagcccgaa ccagggcaag gagaaaatca atgggtgcca
     4441 agaaagtgcg cgtcgaattc gtcgatggtt cggggcaagg cgtgggcggc ttgaacgtca
     4501 aggccacagg ctgcggcgag ctgcaaaccg cccccacggg ccaggccttc ttcctggtgg
     4561 acgaagagaa cttcgccatc accgtcaacg gcgccgaagt ctacaagggc acgctgtcca
     4621 acctgcctga gaagatcgtc ttcaagcaag acggcggcag ctggaaagcg gcctgaacac
     4681 acacgaattt ccaaatctcc tcctccaaag gtgatttttc acgagcggcc cttcggggcc
     4741 gttttttttg gggccgctca cgccttgagg gccggcagcc gcaacaccgc ccgcgcgcca
     4801 cgcgctgcgc gctccaccac tgccacgggc tcgccgcccg gctgcacgtt gcgcaggctg
     4861 acgctgccct ggtgcaaggt ggcgatctcc tgcacgaagg acaggcccag ccccgtgcta
     4921 cgcttgggag accccgggcg cgccagcgag aagaacttgt cgaacacctt gccctcggca
     4981 taaggcggca cgccggggcc gtggtcgcgc acggtgatgc aggccatgcg cccgtcgcgc
     5041 gcgagggtca gcgcgatgtc ggcgctgcca tcgccggcgg gcgtggagaa ctcgaccgcg
     5101 ttgtcgagca ggttgccgat ggcgcggcgc agcagaaagg catcgccttc caccgccagt
     5161 tcgtcctgaa tctggagcac gatctgcagg cgcggcgcgc gcgcgcgggc gccggccacc
     5221 agctcgtgca gcacgggcgc cagctccacg cgctgctggt cttgaagcat gcggcggctt
     5281 tccagcgccg tgagttccat catgcgatcc accacctcct gcatgcggtg cgattcgcgc
     5341 gcgatgttgg cggcgaactt ttgttgctga tcgggcggca tgcccggctc ctgcatcaac
     5401 tccgcagcgc cgcgcacggc cgagatcggg cttttcaact cgtgcgtgag ctgctgcacg
     5461 tagtcctgca cgtagttgcg cccggcaatg gcgtcgcgca cctcttgccc cgccgcccgc
     5521 gcctgcgccc gcagcgcgcg ccacgcccgg cgcgggctga agcggcggcg cccctgctcg
     5581 tcatgccacc acgccgtgcg caggctggcc agcaaatcgc tggtgatgcc caacggccgg
     5641 atcagccaga acgaaagcac cagcgccagc gccaaggcag acagcgccga cacgatgccc
     5701 accgaaatga tgttgccgcg cgccgccgag acaaactggc cgaagctctg caccggcttg
     5761 ccgagcgtga ccacgccgac gatctcgccg ttccagcgga tcggcgcgcc gacgtacatc
     5821 acgctgctgt gcttgtcggc cgccacgtcg cgcgtggtgc gggcgccgta ttcgcccagc
     5881 aaggtgttgc gcacgtcgcg ccagcccgaa tagtccttgc ccagatcgcg ccccgtggag
     5941 tcgtacacca cctggccgtg gcggttggtg acgtaggcgc gcagctcgac gcgcgtcttg
     6001 tgcaggttgt aaatctgcgc cgaaaagcgc cgcgcgtaca ggtcgcgaaa gatgccgccg
     6061 atgcggtccg tcggcagcgc gccgtattcg acgtcttgct cgatcaccgt ggccatgagc
     6121 tgcgcggtct cgatcaggcc ttcttcggcc gactcgcggt agcggggatc gagatcggcc
     6181 agcagttggt acagcaacgc ggccataccg atggcgtaca ccgcgatcag cgcgagcagg
     6241 atgcgggtgc ggcggctcat ggactgcccg tgatgaggga gggcggggca gccctcaccc
     6301 caaccctctc ccagagggag agggagaaaa gacagccgcg cgcagcagcg gcgttgatga
     6361 gactcaggcc gagcgcagcg atggcccgat ccatcttcaa cccctttctg gccgtgccga
     6421 gaagcgcagg gcgtggggcg ggcgcgcagc gcagcatgcg
//
LOCUS       DAOMVO010000127         6378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3271_length_6378_cov_17.612209, whole genome shotgun sequence.
ACCESSION   DAOMVO010000127 DAOMVO010000000
VERSION     DAOMVO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_6378_cov_17.612209"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="sucC"
                     /locus_tag="PKV30_07505"
     CDS             <1..431
                     /gene="sucC"
                     /locus_tag="PKV30_07505"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466206.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRL30094.1"
                     /translation="SKFDLAYISLDGNIGCLVNGAGLAMATMDTIKLFGAEPANFLDV
                     GGGATPEKVTEAFKIMLKNPKVKAILVNIFGGIMKCDTIATGVITASKAVDLKVPLVV
                     RMKGTNEELGKKMLAESGLPIISADTMAEAAQKVVAAVKA"
     gene            446..1339
                     /gene="sucD"
                     /locus_tag="PKV30_07510"
     CDS             446..1339
                     /gene="sucD"
                     /locus_tag="PKV30_07510"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRL30095.1"
                     /translation="MSIYVNKNTKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGESIFNIPIFASVKDAAKETGATCSVIYVPPAGAAAAIWEAVEADLDFVVCITEG
                     IPIRDMLDVRNKMKQKEAKGGKKTLLLGPNCPGIITPDEIKIGIMPGHIHKKGRVGVV
                     SRSGTLTYEAVAQLTELGIGQSTAVGIGGDPINGLKHIDVMKAFNDDPETDAVIMIGE
                     IGGPDEAEAARWCKDHMTKPVVGFIAGVTAPPGKRMGHAGALISGGADTADAKLAVME
                     ESGFKVTRNPSEMGKLLKALL"
     gene            1457..2170
                     /locus_tag="PKV30_07515"
     CDS             1457..2170
                     /locus_tag="PKV30_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRL30096.1"
                     /translation="MEDFFTAHFWVAVGQIIMIDILLGGDNAVVIALACRGLPPHQRT
                     KGIIWGTAGAIVLRVILIAFALALLQIPFLKFVGALLLIWIGVKLIAPEDEDSHANMQ
                     SSDKLWGAIKTIIVADLVMSVDNVIAIAGAAQSAGEKHQLPLVIFGLLVSIPIIVWGS
                     QLVLKLMDRFPIIITLGGMLLGWIAGTMAHTDPALVPYIPQDRAVHYAMGVAGALLVL
                     AIGKLVQSRRRGPPAAGAA"
     gene            2246..3031
                     /locus_tag="PKV30_07520"
     CDS             2246..3031
                     /locus_tag="PKV30_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRL30097.1"
                     /translation="MNFEYSALSDVGRHRANNEDAVMVDPARGIVVLADGMGGYNAGE
                     VASALAVDLIAGELGRWLEEAATVASTRDVRRAMEICVDNANRSIFDAAHTHEAYAGM
                     GTTLVMGVLHQGVFVIGHVGDSRAYRWREGMLARLTRDHSLLQEQIDIGLITEEEAAA
                     SGNRNLVTRALGVEDTVLLDLQETDVAFGDVFLLCSDGLNDMLADDDIAAVLAARTTL
                     EQTAQRLIDLANERGGRDNVSVIVVKAHEPPKKSGVMAKLLRK"
     gene            3119..3763
                     /locus_tag="PKV30_07525"
     CDS             3119..3763
                     /locus_tag="PKV30_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRL30098.1"
                     /translation="MPKLIVSIDGVVVKEFQLTKDRSTIGRRPYNDVVIDNLAVSGEH
                     AVIQMAGGQATLEDLGSTNGTYVNGKAVKKHALVNDDTVEVGKYKIKFLDGDTLSGPA
                     SLTGAGLSVPMGMDEPVSGAAVNAPVDGPSVRVLNGAAVGRQMKLTKIVTTVGKPGLC
                     VASITHKAHGYDLAQVEGQHVAAVNGVTVDGGPITLKNRDQIDLGGIRLEFVDR"
     gene            3760..6033
                     /locus_tag="PKV30_07530"
     CDS             3760..6033
                     /locus_tag="PKV30_07530"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRL30099.1"
                     /translation="MSTRPAPGAASRLWAGFRRHRKRTLATAALVLLAVLHASGIWSF
                     SALQRLDEALYDRRLQLTMPGTLDERVVIIDIDERSLARLGQWPWSRTRVAALVRELL
                     ERQQVAALGMDVVFAEPDGSSGLQHLQQLAREELRGNAAFADWLARTAPTLDYDGVLA
                     AALQSGPVALGYYFSSDRDARRSGTLPRPLAQVDALPPGMLQWDGYGGNIAPLTAAAR
                     AAGFFNSVADPDGKVRAVPLIAGFDGALYESLSLAMLRLGRGEPPLQVRRVGGAPGGA
                     VASVALGGGAQMLQMPLDTRGTALVPYRGPGGPNGGSFRYISAVDVLEGHLAPAQLKG
                     RYALLGFTTPGLMDLRATPVGEAYPGVEVHANLVSGMLDGRIAARPDYAAGYEVALLL
                     LLGAVLAIGLPMLPVGRALALGLGLIGGLLALDLSLYLGAGLVMPLATALVLTLTALA
                     VNMALGYFVESHAKRELARQFATYVPPELVRQMERNPERYSMQARAEELTVMFCDLRG
                     FTSLSETMEPLALQALLNDVLSRLTHVIRAHQGTIDKYMGDCVMAFWGAPVAMPDHAR
                     LAVDAALAMLQSMRELNAERAAQGAPPVLVGIGLNTGVMSVGNMGSDLRRAYTVIGDA
                     VNLAARLEALSRVYDVELIASEATLQRAADAGHVWQELDRVRVKGKQQAVTIHTVRAP
                     AGQGDAALRAELALWRQALRLWRDGAFAEFDTKVNTLRERNANFYLYRLYAGRVASCL
                     QTPPGPGWDGTTVFDAK"
     gene            6050..>6378
                     /locus_tag="PKV30_07535"
     CDS             6050..>6378
                     /locus_tag="PKV30_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL30100.1"
                     /translation="MRVRVLGCSGAIARDCKTTSFLIDQDTLIDAGTGVGDLTREEML
                     RIHDVFLTHSHLDHVACLPLMVDTIAAARAGQPLRVHALPATIAALRQHLFNDLIWPD
                     FTRLPSA"
BASE COUNT         1119 a         2134 c         2108 g         1017 t
ORIGIN      
        1 ccagcaagtt cgacctggcc tacatcagcc tggacggcaa catcggctgc ctggtgaacg
       61 gcgccggctt ggccatggcg accatggaca ccatcaagct gttcggcgcc gagccagcca
      121 acttcctcga cgtcggtggc ggtgccacgc ccgagaaggt gaccgaagcc ttcaagatca
      181 tgctgaagaa ccccaaggtc aaggccatcc tggtcaacat cttcggcggc atcatgaagt
      241 gcgacaccat cgccaccggt gtcatcacgg cgtcgaaagc ggtggacctg aaagtgccgc
      301 tggtggtgcg catgaaaggc accaacgaag aactgggcaa gaagatgctg gccgagtcgg
      361 gcctgcccat catcagcgcc gacaccatgg ccgaagcggc gcaaaaagtc gttgccgccg
      421 tcaaagcctg aaggagccgc aaaccatgtc catctacgtc aacaagaaca ccaaggtcat
      481 cacccagggc atcaccggca agaccgggca gttccacacc gagaagtgcc aggaatacgc
      541 caacggcaag aactgcttcg tggcgggcgt gaaccccaag aaggccggtg agtcgatctt
      601 caacatcccc atcttcgcca gcgtgaaaga cgcggccaag gaaaccggcg ccacctgcag
      661 cgtgatctac gtgcccccgg cgggcgccgc ggccgcgatc tgggaagcgg tggaagccga
      721 cctggatttc gtggtctgca tcaccgaagg catcccgatc cgggacatgc tggacgtgcg
      781 caacaagatg aagcaaaaag aggccaaggg cggcaagaag acgctgctgc tcggccccaa
      841 ctgccccggc atcatcacgc ccgacgagat caagatcggc atcatgcccg gccacatcca
      901 caagaagggc cgtgtcggcg tggtcagccg ctccggcacg ctgacctatg aagcggtggc
      961 ccaactgacc gaactcggca tcggccagtc gactgccgtg ggcatcggcg gcgacccgat
     1021 caacggcctc aagcacatcg acgtgatgaa ggccttcaac gacgaccccg aaaccgacgc
     1081 cgtgatcatg atcggcgaaa tcggcggccc cgacgaggcc gaagccgcgc gctggtgcaa
     1141 ggaccacatg acgaagccgg tggtcggctt catcgctggc gtgacggcgc ctccgggcaa
     1201 gcgcatgggc cacgccggcg cgctgatttc gggcggcgcc gacacggcgg atgccaagct
     1261 ggcggtgatg gaggagtcgg gcttcaaggt cacgcgcaac ccgtccgaga tgggcaagct
     1321 gctcaaggcc ttgctgtagc gcgaacccgc gcgcctgctt gcggcgggcg gcgcagcttg
     1381 caatacagcc ggatgccttg gcaggtcatc cggctgtatt atttgtgtcg cgtccattcc
     1441 atcgaaaaga gacaacgtgg aagacttctt caccgcccat ttctgggtcg ctgtcggtca
     1501 aatcatcatg atcgacatcc tgcttggtgg cgacaacgcc gtggtcattg cgctggcctg
     1561 tcgcggactg ccgccgcacc agcgcaccaa gggcatcatc tggggcactg ccggcgccat
     1621 cgtgctgcgc gtgatcctga tcgcctttgc gctggcgctg ctgcagattc cgtttctcaa
     1681 gttcgtcggc gcgctgctgc tgatctggat cggcgtcaag ctgattgcgc cggaggacga
     1741 agattcgcac gccaacatgc agagcagcga caagctgtgg ggcgccatca agaccatcat
     1801 cgtggccgac ctggtgatga gcgtcgacaa cgtcatcgcc atcgccggcg cggcccagag
     1861 cgcgggcgag aagcaccagt tgccgctggt catcttcggc ctgctggtga gcatcccgat
     1921 catcgtctgg ggcagccagc tggtgctcaa gctgatggat cgctttccga tcatcatcac
     1981 gctgggcggc atgctgctgg gctggattgc cggcaccatg gcgcacaccg atccggcgct
     2041 ggtgccctac atcccgcagg atcgcgcggt gcactacgcg atgggcgtcg cgggcgcctt
     2101 gctggtgctg gccatcggca agctggtgca aagccggcgc cgcggtccgc cagcggccgg
     2161 cgcggcttga aggatcgcga ttgaaagcac catcggccgc ccgtgatcag gtcgtcgtgg
     2221 ccgcttgccg gcgcgagggc ggcgcatgaa tttcgagtac agcgccctgt cggatgtcgg
     2281 gcgccaccgc gccaacaatg aagacgcggt gatggtcgac cccgcgcgcg gcatcgtggt
     2341 gctggccgat ggcatggggg gttacaacgc cggtgaagtg gccagcgcgc tggcggttga
     2401 cctgatcgcc ggcgaactgg gccgctggct ggaagaggcg gcgaccgtgg ccagcacgcg
     2461 cgatgtgcgg cgggccatgg aaatctgcgt cgacaacgcc aaccgatcca tcttcgacgc
     2521 cgctcacacg cacgaagcct acgccggcat gggcacgacg ctggtcatgg gtgtgctgca
     2581 ccagggcgtg ttcgtgattg gccacgtcgg cgattcacgc gcctaccgct ggcgcgaagg
     2641 catgctggcc aggctgacgc gcgaccattc cctgctgcag gaacagatcg atatcggcct
     2701 gatcaccgaa gaagaggcgg cggcctcggg caaccgcaat ctggtgacac gcgcgctcgg
     2761 cgtcgaggac acggtgctgc tcgatctgca ggagaccgac gtcgccttcg gcgacgtgtt
     2821 cctgctgtgc tccgatggct tgaacgacat gctggccgac gacgacattg cggccgtgct
     2881 ggccgctcgc acgacgctgg aacagaccgc acagcggctg attgacctgg ccaacgaacg
     2941 cggcggccgc gacaacgtgt cggtcatcgt cgtgaaagcg catgaaccgc ccaaaaagtc
     3001 cggggtgatg gccaaattgc tgagaaaatg agcgcaagat ggcttgcctt cggcccatga
     3061 aatcggcttc aatgggcgac ccatcaccag cacaagctac aaaaacggga gtcatttcat
     3121 gccgaagttg atcgtctcga tcgacggcgt tgtcgtcaag gaattccagc tgaccaagga
     3181 tcgctcgacc attggccggc ggccctacaa cgacgtggtc atcgacaacc tggccgtcag
     3241 tggcgagcac gccgtcatcc agatggcggg cggccaggcc acgctcgagg atctgggcag
     3301 caccaatggc acctacgtca atggcaaggc ggtcaagaaa catgcgctgg tgaatgacga
     3361 cacggtcgaa gtcggcaagt acaagatcaa gtttctggat ggcgacacct tgtccggccc
     3421 agccagtctg accggcgcgg gcctgtcggt gccaatgggg atggatgagc cggtcagtgg
     3481 tgctgctgtc aatgcgccgg tcgatggacc gagcgtgcgg gtgcttaacg gcgccgcggt
     3541 cgggcgccag atgaagctga ccaagatcgt caccacggtg ggcaagccgg ggctttgcgt
     3601 ggcctccatc acgcacaagg cgcacggcta cgacctggct caggtggagg ggcagcatgt
     3661 ggcagcggtc aacggcgtga ccgtggatgg cggcccgatc accctcaaga accgcgatca
     3721 gatcgatctg ggcggcattc gtctggagtt cgtcgaccgt tgagcacgcg cccggcaccg
     3781 ggcgcggcca gcaggctctg ggcgggcttt cggcgccatc gcaaacgcac gctggccacc
     3841 gcagcgctgg tgctgctggc cgtattgcac gcttccggaa tatggagctt ctcggcgctg
     3901 cagcgcctgg acgaagccct gtacgaccgg cgtctgcagc tgaccatgcc cggcacgctc
     3961 gacgagcgcg tggtcattat cgacatcgac gagcgcagcc tggcgcggct gggccaatgg
     4021 ccctggagcc gcacccgcgt ggccgcactg gtgcgcgagt tgctcgaacg tcaacaggtg
     4081 gccgcactgg gcatggacgt ggtgtttgcc gagccggatg gcagctccgg cctgcagcac
     4141 ctgcagcaac tggcacgtga agagctgcgc ggcaacgccg cgtttgcaga ttggctggcc
     4201 cgcacggcgc cgacgctcga ctacgacggc gtgctggccg cggcgttgca aagcggcccc
     4261 gtggcgctgg gctactactt cagcagcgac cgcgacgcgc gccgctcggg cacgctgccg
     4321 cgcccgctgg cacaggtcga tgccctgccg cccggcatgc tgcagtggga cggctacggc
     4381 ggcaacatcg cgcccttgac ggctgccgcg cgcgccgccg gcttcttcaa ctcggtggcc
     4441 gatccggacg gcaaggtgcg cgccgtgccg ctcattgccg ggtttgacgg cgcgctctac
     4501 gaatcgctgt cactggccat gctgcgcctg gggcggggcg agccgccgct gcaagtgcgc
     4561 cgcgtcggcg gcgcgccggg tggcgcggtg gcgtccgtcg cgttgggcgg cggcgcgcag
     4621 atgctgcaaa tgcccctcga cacgcgcggc accgccctcg tgccctaccg cggaccaggc
     4681 gggccgaacg gtgggtcgtt tcgctacatc tcggccgtcg acgtgcttga aggccacctc
     4741 gcgcccgccc aactcaaggg ccgttacgcg ctgttgggct tcaccacgcc gggcctgatg
     4801 gacttgcgcg ccacgccggt gggcgaggcg taccccggcg tcgaagtgca cgccaacctg
     4861 gtctccggca tgctcgacgg ccgcatcgca gcgcggcccg actatgccgc cggctatgaa
     4921 gtggccctgc tgctgctgct gggcgccgtg cttgccatcg gcctgcccat gttgccggtc
     4981 gggcgcgcgc tggcgctggg gctggggctg atcggcgggc tgcttgcgct cgatttgtcg
     5041 ctctatcttg gcgccggcct ggtcatgccg ctggccaccg cgctggtgct gacgctgacc
     5101 gcgctggccg tcaacatggc gctcggctac ttcgtcgaaa gccacgccaa gcgcgaactg
     5161 gcgcggcagt tcgccaccta cgtgccgccc gagctggtgc gccagatgga gcgcaacccc
     5221 gagcgctaca gcatgcaggc gcgcgccgaa gagctcaccg tgatgttctg cgatctgcgc
     5281 ggcttcacca gcctgtcgga aaccatggag ccgctggccc tgcaggcgct gctgaacgac
     5341 gtgctctcgc gcctcaccca cgtcatccgc gcgcaccagg gcaccatcga caaatacatg
     5401 ggcgactgcg tcatggcctt ctggggcgcg cccgtggcca tgcccgacca cgcgcgcctg
     5461 gccgtcgatg ccgcgctggc catgctgcaa tccatgcgcg agctgaacgc cgagcgcgcc
     5521 gcccagggcg cgccaccagt gctggtcggc atcgggctca acaccggcgt catgtcggtc
     5581 ggcaacatgg gctcggacct gcggcgcgcc tacaccgtca tcggcgacgc cgtgaatctg
     5641 gccgcgcggc tggaggcttt gtcacgcgtc tacgacgtcg aactcatcgc cagcgaagcc
     5701 accttgcaac gcgccgccga cgccggccac gtctggcagg aactggaccg cgtgcgcgtc
     5761 aagggcaagc aacaggcggt caccatccac accgtgcgcg cccccgccgg ccagggcgac
     5821 gctgcgctgc gcgcagaact cgccctgtgg cgccaggccc tgcgtttgtg gcgcgacggc
     5881 gcatttgctg agtttgatac aaaagttaac acgctgcgcg aacggaatgc caacttttac
     5941 ctttaccggc tgtacgccgg gcgggtagcc tcctgtcttc aaacgccgcc cggccccggc
     6001 tgggacggca ccacggtatt cgacgccaaa tgagcgcagg gagcgattca tgagggtgcg
     6061 tgtgctgggc tgctccggcg ccatcgcccg cgattgcaag acaacgtcgt tcctgatcga
     6121 ccaggacacg ctcatcgacg ccggcaccgg cgtcggcgac ctcacgcgcg aggagatgct
     6181 gcgcatccac gacgtcttcc tcacccactc gcacctcgac cacgtggcct gcctgccgct
     6241 catggtcgac accatcgccg ccgcccgtgc cggccagccg ctgcgcgtgc atgccctgcc
     6301 cgccaccatc gccgcgctgc gccagcacct gttcaacgac ctgatctggc ccgacttcac
     6361 ccgcctgccc tcggccga
//
LOCUS       DAOMVO010000128         6300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3320_length_6300_cov_15.634428, whole genome shotgun sequence.
ACCESSION   DAOMVO010000128 DAOMVO010000000
VERSION     DAOMVO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_6300_cov_15.634428"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..1295
                     /locus_tag="PKV30_07540"
     CDS             477..1295
                     /locus_tag="PKV30_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30101.1"
                     /translation="MTAPTPDALVELRDVTFGYGERPVLRDLALKVPRGKVTALMGAS
                     GGGKTTVLRLIGGQYRAQQGDVLFDGRDVGKMSEEQLYAARRRMGMLFQFGALFTDLS
                     VLENVAFPLREHTKLPEALIRDIVLMKLGAVGLRGARELMPAQVSGGMARRVALARAI
                     ALDPELVMYDEPFAGLDPISLGTAAQLIRKLNDALGITSIVVSHDVQETFLIADQVII
                     LADGRVAAQGTPDEVRASTDPLVRQFVGMAVDGPVQFHYPGPTVEQDFGIEVAP"
     gene            1292..2074
                     /gene="mlaE"
                     /locus_tag="PKV30_07545"
     CDS             1292..2074
                     /gene="mlaE"
                     /locus_tag="PKV30_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HRL30102.1"
                     /translation="MSWWRPTDVGFATRSQLADIGYAARFFWRLVGLLGPSLRRFSLV
                     RDQIHFLGNYSLAIIAVSGLFVGFVLALQGYNILQRYGSAEAVGMMVALSLLRELGPV
                     VTALLFAGRAGTSLTAEIGLMKAGEQFSAMEMMAVDPIRRIIAPRFWAGVIVVPLLTA
                     VFNAVGILGGWIVSVPMIGVDPGAFWSQMQGGVDVWADLGNGVVKSVVFGITVTFVAL
                     LQGYVAKPTADGVAHATTRTVVMASLMVLGLDFLLTALMFSF"
     gene            2085..2567
                     /gene="mlaD"
                     /locus_tag="PKV30_07550"
     CDS             2085..2567
                     /gene="mlaD"
                     /locus_tag="PKV30_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRL30103.1"
                     /translation="MGRSKNDLWVGLFVLIGLAALVFLALQSANLLSLNFQRGYRITA
                     NFDNIGGLKPKAAVRSAGVVVGRVADIRFDDERYQARIDLDMDTRFKFPKDSALKILT
                     SGLLGEQYIGIEAGASDQNLAAGDNVTATQSAVVLENLISQFLYNSAANNASSGGSGK
                     "
     gene            2667..3608
                     /locus_tag="PKV30_07555"
     CDS             2667..3608
                     /locus_tag="PKV30_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRL30104.1"
                     /translation="MSGQNNSAWRRAQWGAGVLALVVVSGCTSLPDGASRDPRDPWEG
                     YNRSMFRFNDAVDTAVLKPVATAYRDVVPQPVRTGVGNFFGNLSDAWSFVNNVLQAKP
                     EGAMSSFWRVVINSTMGLGGVLDPATEMRLERRREDFGQTLGRWGVPDGPYFVLPVLG
                     PSTVRDSAALPVDFYGSPIGHVSDVAVRNSLYGVQAVNTRANVLGATELLESAALDPY
                     TFQRDAYLQKRRNDIHDGNPPEDEERYDLEEGEAPTADAPAPAASGAAPARAPTRAPR
                     RVLRRPGARTAPNAPAAAASGVPAAPAPSASQPAPVK"
     gene            3724..4386
                     /locus_tag="PKV30_07560"
     CDS             3724..4386
                     /locus_tag="PKV30_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30105.1"
                     /translation="MTSQAPRRHFVRLTASALGALTLLAAAPWARAAEEAPDAMIQRL
                     SDDLLQTIRADKTLQTGDVDRVMAVVDSKVMPNVNFTRMTASATGPSWRKATPDQRQR
                     LQQEFKTLLVRTYAGALKQVSDQSVEVKPLRAAPNDKEVIVRTLVKSKGDPVQLDYRL
                     ERTPGQGSGWKIYDLNVLGVWLVDNYRPQFSQQLNAGGVDALINSLAERNRSNASPSG
                     KS"
     gene            4403..4705
                     /locus_tag="PKV30_07565"
     CDS             4403..4705
                     /locus_tag="PKV30_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRL30106.1"
                     /translation="MLVLPAELTHVQAQTCLNVLLQAAEAEAAPQVLVDASALTRFDS
                     SALAVLLECRRECLQDGKGFAVRGLSPRLRELAQLYGVLALLPDPAAAESAVLAAA"
     gene            4787..5752
                     /locus_tag="PKV30_07570"
     CDS             4787..5752
                     /locus_tag="PKV30_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30107.1"
                     /translation="MPAISFQSVSKVYPASGKPGSTPFQALDDVSFDIQQGEFFGLLG
                     PNGAGKTTLISILAGLTRASSGHVKVYGFDVQNDFAQARRHLGIVPQELVFDPFFTVR
                     EALRFQSGYFGLHKNDDWIDELLTGLGLADKAKANMRQLSGGMKRRVLVAQALVHRPP
                     VIVLDEPTAGVDVELRQTLWQFVDQLNRQGHTVLLTTHYLEEAEALCQRIAMLKRGQV
                     IALERTSTLLANAAASVLHFKTDSTLPPALAAQARVTGRIVELPAYAADDVERHLSAL
                     REAGVRVEDLEIRKADLEDVFLKMMGATPEAPPPAGRADLAEVEA"
     gene            5824..>6300
                     /locus_tag="PKV30_07575"
     CDS             5824..>6300
                     /locus_tag="PKV30_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL30108.1"
                     /translation="MLLKKEVLRFWRVAFQTIAAPVLTAVLYLMIFGHVLSDRVQVYP
                     GVGYVAFLVPGLVMMAVLQNAFANSASSLVQSKIMGNLVFTLLTPLSARDWFIAYVGA
                     AIARGLLVGLGVYLVTLLYAPPRVASLPWVLAFALLGAALMGVLGLLAGLWADKFDQ"
BASE COUNT          984 a         2089 c         2186 g         1041 t
ORIGIN      
        1 cagggcggcc agggcctcgg ttcgcgcgga ggcggcccac agcaagcgca tgtcgcccgc
       61 tgcattcaac gccgcgacca aagcgatgcg cgaggccgcg tcgtcctcca ccgtcgccac
      121 gcgaataggc gcgggcgcgg cggcggcggc gggcgggtcg gtgttgggca tgggccgcca
      181 tcttgaccca agccggcgtg ctgacgcatg tcgccacttc tggcgacatg tcacggcctg
      241 accggccctc gcagacggcg ctgcgggagc catgcgcagc gcggcggacg caccaacgtc
      301 cgtcggcccg gcaacgcgtg atctatgctc cacaattgat agctggcagc gcacatccgt
      361 cctgcgcaaa gccccattca ggcatgaagc atgtggccgt tctgtgcgct gtattcgcgg
      421 gatgaatcga tcccgtatca ttgaccggcc ggctccccag ccggtttttt gatgccatga
      481 cagcccccac ccccgacgcc ctggtcgagc tgcgcgacgt gaccttcggt tacggcgagc
      541 gcccggtgct gcgcgatctg gcgctcaagg tgccgcgcgg caaggtcacg gcgctgatgg
      601 gcgcgtcggg cggcggcaag acgacggtgc tgcggctgat tggtgggcaa taccgcgcgc
      661 agcagggcga cgtgctgttc gacgggcgcg atgtcggcaa gatgtctgaa gagcagcttt
      721 atgccgcgcg gcgccgcatg ggcatgctgt ttcagtttgg cgcgctgttc accgacttga
      781 gcgttttgga gaacgtggcc tttcccctgc gcgagcacac caagctgccc gaggcgctga
      841 tccgcgacat cgtgctgatg aagctgggcg cggtgggcct gcgcggcgcg cgcgaactga
      901 tgccggcgca ggtatccggc ggcatggcgc gacgcgtggc gttggcgcgg gccatcgcgc
      961 tcgaccccga gctggtgatg tacgacgagc cctttgccgg gctggatccg atctcgcttg
     1021 gcaccgcggc gcagctgatc cgcaagctga acgatgcgct gggcatcacc agcatcgtgg
     1081 tgtcgcacga cgtgcaagag acgttcttga tcgccgacca ggtgatcatc ctggccgacg
     1141 gccgggtggc ggcgcagggc acgcccgacg aggtgcgggc cagcaccgat ccgctggtgc
     1201 ggcagttcgt cggcatggcg gtggatgggc cggtgcagtt tcattacccg gggccgacgg
     1261 tggagcagga ttttggcatc gaggtggcgc catgagctgg tggcggccga ccgacgtggg
     1321 ctttgcgacg cgatcgcaac tggccgacat cggctacgcg gcgcgctttt tctggcgcct
     1381 ggtcggcctg ctggggccga gcttgcgccg cttttcgctg gtgcgcgacc agattcactt
     1441 tctgggcaac tattcgctcg ccatcatcgc ggtgtcgggg ctgtttgtcg gcttcgtgct
     1501 ggcgctgcag ggctacaaca tcctgcagcg ctacggctcg gccgaagcgg tgggcatgat
     1561 ggtggcgctg tcgctgctgc gcgagctggg gccggtggtc acggcgctgc tgtttgccgg
     1621 ccgcgcgggc acgtcgctga cggccgaaat cgggctgatg aaggcgggcg aacagttcag
     1681 cgccatggag atgatggcgg tcgacccgat tcgccgcatc atcgcgccgc gtttctgggc
     1741 cggcgtgatc gtggtgccgc tgctcacggc ggtgttcaac gccgtgggca ttctgggcgg
     1801 ctggatcgtc agcgtgccga tgatcggcgt cgatccgggc gcattctgga gccagatgca
     1861 gggcggcgtc gatgtgtggg ccgacctggg caacggcgtg gtcaagagcg tggtgttcgg
     1921 catcacggtg acctttgtcg cgctgctgca gggctacgtg gccaaaccca cggccgacgg
     1981 cgtggcgcac gcgaccacgc gcacggtggt gatggcctcg ctgatggtgc tggggctcga
     2041 cttcttgctg acggccttga tgttcagttt ttaggaagat ttctatggga cgctccaaaa
     2101 atgatctctg ggtgggcctg ttcgtgctga tcgggctggc ggcgctcgtg tttctggcgc
     2161 tgcagtcggc caatctgttg tcgctcaact tccagcgcgg ctaccgcatc acggcgaact
     2221 tcgacaacat cggcggcttg aagcccaagg cggctgtgcg cagcgcgggc gtggtggtcg
     2281 ggcgagtggc ggacatccgc ttcgacgacg agcgctacca ggcgcggatt gacctcgaca
     2341 tggatacgcg cttcaagttt ccaaaagaca gcgcgctcaa gatcctgacc agcggtttgc
     2401 tgggcgagca atacatcggc atcgaggccg gcgcgtcgga ccaaaacctg gcggccggcg
     2461 ataatgtcac cgccacacag tcggccgtgg tgctggagaa cctgatcagc cagtttctct
     2521 acaacagcgc cgccaacaac gcatcctctg gcgggagtgg caaataagcg gtgcatgcgg
     2581 cccaatcggg acgtggcgca cgcaaatggc cgcgcgatgc gcggcagcag caaaatgaag
     2641 accggatggg acaccaaggt agacggatga gcgggcagaa caacagcgcc tggcggcgcg
     2701 cgcaatgggg cgccggcgtg ctggcactgg tggtggtgag cggctgcacc agcctgccgg
     2761 acggcgcatc acgcgacccg cgcgatccgt gggaaggcta caaccgcagc atgttccgct
     2821 tcaacgacgc ggtcgacacg gcggtgctca agccggtggc cacggcgtat cgcgacgtgg
     2881 tgccgcagcc ggtgcgcacc ggcgtcggca acttcttcgg caacctgagc gacgcctggt
     2941 cgttcgtcaa caacgtgctg caggccaagc cggaaggggc catgtcttcc ttctggcgcg
     3001 tggtcatcaa cagcaccatg ggcttgggcg gcgtgctcga cccggccacc gagatgcggc
     3061 tggagcgccg gcgcgaggat ttcggccaga cgctcggccg ctggggtgtg cccgacggcc
     3121 cgtatttcgt cttgccggtg ctcggcccct cgacggtgcg cgattcggca gccttgccgg
     3181 tggatttcta cggcagcccg atcggtcacg tctcggacgt ggcggtgcgc aattcgctgt
     3241 acggggtgca ggccgtcaac acccgcgcca atgtgctcgg cgcgaccgag cttctggagt
     3301 cggccgcgct cgacccctac accttccagc gcgacgccta cctgcaaaag cgccgcaacg
     3361 acatccacga tggcaatccg cccgaggacg aagagcgcta cgacctggaa gaaggcgaag
     3421 cgcccacggc cgatgcgccg gcccctgccg cttcgggcgc cgcgcccgcg cgtgcgccaa
     3481 cgcgcgcgcc gcgccgcgtg ttgcgccgac cgggtgcgcg caccgcgccc aacgcccccg
     3541 ccgcagcggc ttccggtgtg cctgcggcgc ccgcgccgtc ggcgtcacag cccgcgccgg
     3601 tcaaataagg ctgccgggca acgtcgccag cggcacaact tgtaacagcg ccggggaacc
     3661 gcccggcgca actgtattca aaagacccaa gggcgcgccg tgcgcccgga ggacaccaga
     3721 cccatgacat cgcaagcccc tcgccgccac tttgttcgac tgaccgccag tgcgctgggc
     3781 gcgctgacgc tgctggccgc cgcaccctgg gcgcgggcgg ccgaagaggc gcccgatgcc
     3841 atgatccagc gcctgtcgga cgacttgctg cagaccattc gcgccgacaa gacgttgcag
     3901 accggcgacg tcgaccgcgt gatggcggtg gtcgacagca aggtcatgcc caacgtcaac
     3961 ttcacgcgca tgaccgcatc ggccaccggc ccgagctggc gcaaggccac gccagaccag
     4021 cgccagcgcc tgcagcagga gttcaagacc ctgctggtgc gcacctatgc cggcgcgctc
     4081 aagcaggtga gcgaccagtc ggtcgaagtg aagccgctgc gcgccgcgcc caacgacaag
     4141 gaagtcatcg tgcgcacgct ggtcaagagc aagggcgacc ctgttcaact cgactatcgg
     4201 ctggagcgca cacccggcca gggcagcggc tggaagatct acgacctcaa tgtgctgggc
     4261 gtctggctgg tcgacaacta ccgcccgcag ttctcgcagc agctcaatgc gggcggcgtg
     4321 gacgcgctca tcaattcgct ggccgagcgc aatcgcagca acgccagccc cagcggcaag
     4381 agctgattca caacgttccc ccatgctggt gctgcccgcc gaactcacgc acgtgcaggc
     4441 gcagacctgc ctgaacgtgc tgctgcaggc cgccgaagcc gaggcggcgc cgcaggtgct
     4501 ggtcgatgcc tcggcgctga cgcgcttcga ctcgtcggcg ctggcggtgc tgctggaatg
     4561 ccgccgcgaa tgcctgcagg acggcaaggg ctttgccgtg cgcggcttgt cgccgcgcct
     4621 gcgtgaactg gcgcagctct acggcgtgct ggccttgctg cccgatcccg ctgcggctga
     4681 gtcggcggtg ctggcggccg cctgagcccg cgcgccgtgc ggcgccggca tcaaccttgc
     4741 gaccagccgg atgacggcaa aaacgtaaaa tggcgggttg cccatgctgc ccgccatttc
     4801 ctttcagtcg gtgtccaagg tttacccagc ctcgggcaaa cccggctcca cccctttcca
     4861 ggcgcttgat gacgtcagct ttgacatcca gcagggcgag ttctttggcc tgctcggccc
     4921 caacggcgcc ggcaagacga cgctgatctc gatcctggcg ggcctcacgc gcgccagctc
     4981 gggccacgtc aaggtgtacg gcttcgatgt gcaaaacgac ttcgcccagg cgcgccgcca
     5041 tctgggcatc gtgccgcaag aactggtgtt cgaccccttc ttcaccgtgc gcgaggcgct
     5101 gcgctttcaa tccgggtatt tcggcctgca caagaacgac gactggatcg acgaactgct
     5161 gaccggtctc ggcctggccg acaaggccaa ggccaacatg cgccagctgt cgggcggcat
     5221 gaagcgccgc gtgctggtgg cgcaggcgct ggtgcaccgg ccgcccgtca tcgtgctcga
     5281 tgaacccacg gccggcgtcg atgtcgagct gcgccagacg ctgtggcagt tcgtcgacca
     5341 gctcaaccgc cagggccaca ccgtgctgct gaccacccac tacctcgaag aggccgaggc
     5401 gctgtgccag cgcatcgcca tgctcaagcg cggccaggtg atcgcgctgg agcgcaccag
     5461 cacgctgctg gccaacgccg ccgccagcgt gctgcacttc aagaccgaca gcacgctgcc
     5521 gcccgcgctg gccgcgcagg cgcgcgtcac gggccgcatc gtcgaactgc cggcctatgc
     5581 ggcggacgat gtcgagcgtc acctgagcgc gctgcgcgaa gccggcgtgc gcgtggaaga
     5641 cctggaaatc cgcaaggccg acctggaaga tgtgttcctc aagatgatgg gcgctacgcc
     5701 tgaggcgccg ccgcccgccg gccgcgccga cttggccgag gtggaggcat gagcgggcaa
     5761 gctcacgccc ccctcgcaag gccgcccttg caggccgtgg cggcacccaa cgacggctgg
     5821 aagatgctgc tcaagaagga agtgctgcgc ttttggcgcg tcgccttcca gaccatcgcc
     5881 gcgccggtgc tgacggcggt gctgtacctg atgatcttcg gccacgtgct gagcgatcgc
     5941 gtgcaggtgt atccgggcgt cggctacgtc gcctttctgg tgccgggcct ggtgatgatg
     6001 gccgtgctgc agaacgcctt tgccaacagc gcgtccagcc tggtgcaaag caagatcatg
     6061 ggcaacctgg tgttcacgct gctcacgccg ctgtcggcgc gcgactggtt cattgcctac
     6121 gtgggcgcgg ccattgcgcg cgggctgctg gtggggctgg gcgtgtacct ggtcacgctg
     6181 ctgtacgcgc cgccgcgcgt cgcctcgctg ccgtgggtgc tggcgttcgc gctgctcggc
     6241 gcggcgctga tgggcgtgct gggcctgctg gccgggctgt gggccgacaa gttcgaccag
//
LOCUS       DAOMVO010000129         6267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3355_length_6267_cov_15.824050, whole genome shotgun sequence.
ACCESSION   DAOMVO010000129 DAOMVO010000000
VERSION     DAOMVO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3355_length_6267_cov_15.824050"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKV30_07580"
     CDS             <1..668
                     /locus_tag="PKV30_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRL30109.1"
                     /translation="VVKKPGYAPHEDFTPLGGFATFVNGLAVSPGTPVKSVPEFVEWV
                     KAQGGKGSVGIPAPASTPEFLVQLIGKRYGLDLVAVPYKGSAPMMADMLGNQIPAGVG
                     SVQDFIEYQRAGKLQMIAVLGAQRQAAMPDVPTLAELGFKGLEDTPFYGIWAPKGTPT
                     TFINGFSQALDKVVAMPDVRKSLTDMGLTVGYMSPQQLDKRERAYTQVWSRIIKDSGF
                     QPQ"
     gene            complement(877..1554)
                     /locus_tag="PKV30_07585"
     CDS             complement(877..1554)
                     /locus_tag="PKV30_07585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL30110.1"
                     /translation="MGTLYLVRHGQASFGADDYDQLSDLGRRQSVRLGQYLRERYGDG
                     LRFNAVLTGTLRRQIGTWQGIAEGGGFDAPHVSWPGLNEYDSHAVIAAIHNEPLPKPD
                     TPELYRHHFRLLRDGLTAWMQGRVQPVGMPSYVDFRAGVIGALDHVRQNFENEVLLVS
                     SGGPIATAVAHVLDAPFEASIELNLRIRNTAVTEFAFNPKRHQLITYNTLPHLDAPPY
                     RDWLTHA"
     gene            complement(1794..2756)
                     /locus_tag="PKV30_07590"
     CDS             complement(1794..2756)
                     /locus_tag="PKV30_07590"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HRL30111.1"
                     /translation="MSTLPKLGEIEAASAVVYREFPPTPQYRWALLSERLGTDCWVKH
                     ENHTPVGAFKIRGGLTFFDALARRGELPREVVSATRGNHGQSIGWAARTHGVACTIVV
                     PRGNSVEKNAAMRALGVHLIEHGDDFTEASDHAAQLAEARGALRVPSFHADLVSGVAT
                     YWWEFLKAVPQLDVAYVPIGLGSGACAAIAAKLALGHKARIVGVVSRHATTYADSLAA
                     GRVVEAPVSTQIADGMAVRRADAAALAAMTGRLDHVVQVSDDEVAGAMRALYADTHNA
                     AEGAGAAALAAALQGRSNLRGQCVGIALTGANVDASVFAEVLGR"
     gene            complement(2753..3388)
                     /locus_tag="PKV30_07595"
     CDS             complement(2753..3388)
                     /locus_tag="PKV30_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL30112.1"
                     /translation="MFTLNELLLFAAAALVLVLTPGPNMVYCVSRSLTQGPRAGLLSL
                     AGVVLGFLMHLFAAALGLTALLLAVPMTFDVIRIAGALYLLWMAWQAVRSGGAAPFQA
                     RRLPHDGPKKLFAMGFMTNVLNPKGAMFYLAFFPQFLHPERGSVLLQSLLLGAIQIAV
                     SAGINALLVLFAGGVSGFLNRSPRRVNAQRYLMGSVLGLLALRILNEKKPT"
     gene            complement(3388..4095)
                     /locus_tag="PKV30_07600"
     CDS             complement(3388..4095)
                     /locus_tag="PKV30_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL30113.1"
                     /translation="MHDRARLDHLVVMAGTLAEGAAWCEATLGVLPGAGGEHPLFGTH
                     NRLLSVASDAFPLAYLEIIAINPEAAGARQTSAGGLKRWFDMDDEALRAQLRRHGPRL
                     IHWVARVPDVQDAAARLAMLEIDRGEVLRANRMTPAGLLEWQITVRPDGRRLFDGCLP
                     TLIEWAGVHPATDMPPSGLTLRSLTLRHPRAAALGEACTAVGLADVAIAPDVHASIDV
                     VLATPRGEVVLSSAEGG"
     gene            complement(4101..5003)
                     /locus_tag="PKV30_07605"
     CDS             complement(4101..5003)
                     /locus_tag="PKV30_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRL30114.1"
                     /translation="MRARPFAQIDVFTAAPGYGNPVAVVLDGAGLDDAAMQRFARWTN
                     LSETTFVRPPTDPQADYALRIFTPGGELPFAGHPTLGSCFAWLAAGGQPKSADRIVQQ
                     CAKGLIPIRRDGERLAFAAPPSVRSDPAPALLADIAQALGVEPAQILRAQRLDNGPVW
                     LTLQLDSADTVLALEPDHARLAALGQKVGVIGVYPAPSAIELVSRSSREARAFGTPAP
                     APLIEVRAFAAPIGVPEDPVTGSLNASLAQWLIEAGELPPRYVAAQGTALGRAGRVHV
                     ERDAEGQVWIGGDCVGVIQGQVTL"
     gene            complement(5069..6253)
                     /locus_tag="PKV30_07610"
     CDS             complement(5069..6253)
                     /locus_tag="PKV30_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL30115.1"
                     /translation="MAERAHAMNPSAIREILKVTERPGILNFAGGLPSPETFPIEAMR
                     AACASVLAEGSVVAKPSLQYASSEGLPELRQWVSAELGKQGATVSPDQVLITTGSQQG
                     LDLIAKVLIDKDSRLLVESPTYLGALQAFTPMQPAVESIESDSGGVCPSALREALQKG
                     ERPRFIYLLPNFQNPTGRTMDEARREAVIAVCREHGLPVVEDNPYGELWFDAPPPKPL
                     LARWPEGVVYLGSFSKILAPGLRLGYIVAPPALYPKLLQAKQAADLHTPGFNQRVVAE
                     VIKDGFLDQHVPTIRARYHAQRDAMLAALARELGPTGAEWTQPVGGMFVWVRLPERLN
                     AQALLPKAVDAGMAFVPGAPFYAGEPDARALRLSFVTSTPEQIDKGMAALGRVVQQAM
                     CE"
BASE COUNT         1039 a         2107 c         2146 g          975 t
ORIGIN      
        1 aggtggtcaa gaagcccggc tacgcgccgc acgaggactt cacgccgctg ggcggcttcg
       61 ccaccttcgt caacggtctg gcggtgtcgc ccggcacgcc ggtcaagtcg gtgcccgagt
      121 tcgtcgagtg ggtgaaggcg cagggcggca agggctcggt cggcatcccg gcaccggcct
      181 cgacgcccga gttcctggtg cagttgatcg gcaagcgcta cgggctggac ctggtggcgg
      241 tgccctacaa gggcagcgcg ccgatgatgg ccgacatgct gggcaaccag attccggccg
      301 gcgtcggctc ggtgcaggac ttcatcgaat accagcgcgc cggcaagctg cagatgattg
      361 ccgtgctcgg cgcccagcgc caggccgcca tgcccgatgt gcccacgctg gccgagctgg
      421 gcttcaaggg gctggaagac acgcccttct acggcatctg ggcgcccaag ggcacgccca
      481 ccaccttcat caacggcttc agccaggcgc tggacaaggt ggtcgccatg cccgacgtgc
      541 gcaagtcgct cacagacatg gggctgacgg tgggctacat gagcccgcag cagctcgaca
      601 agcgcgagcg cgcctacacg caggtgtggt cgcgcatcat caaggacagc ggcttccaac
      661 ctcaatgatg ttgggctgag tcggagcagc cggacgcaaa ggacgcagaa attacgcaga
      721 ggacgcagaa gaaagtccag aaaacttgtt tttggtttct tttgcgtcct ctgcgaatcc
      781 tttgcgtcct ctgcgtccgg ctgtttgtat ttcaagcaaa aaaatcgccc aacgcttgcc
      841 agatcagcgc aagcagctac gacaaacgaa gcatcctcaa gcgtgcgtca gccagtcgcg
      901 gtacggcggc gcgtccaggt ggggcaaggt gttgtaggtg atgagctggt ggcgcttcgg
      961 gttgaaggcg aactcggtca ccgcggtatt gcggatgcgc aggttcagct cgatgctggc
     1021 ttcgaacggc gcgtccagca cgtgcgccac ggcggtggca atcgggccgc cgctgctgac
     1081 cagcaacact tcgttctcga agttctgccg cacatgatcg agcgcgccga tcacgccagc
     1141 gcgaaagtcg acgtagctgg gcatgcccac aggctgcaca cggccctgca tccaggccgt
     1201 gaggccgtcg cgcagcaggc ggaagtggtg gcggtacagc tcgggcgtgt cgggtttggg
     1261 cagcggctcg ttatgaatcg cggcgatgac ggcgtggctg tcgtattcgt tcaggccggg
     1321 ccacgagaca tgcggcgcat cgaacccgcc gccttcggcg atgccctgcc aggtgccgat
     1381 ctgacggcgc agcgtgccgg tcagcacggc gttgaagcgc aagccatcgc cataccgctc
     1441 gcgcaggtac tgacccagcc gcacgctctg gcgccggccc agatcgctca gctggtcgta
     1501 gtcgtcggca ccaaagctgg cctggccgtg gcgcacgagg taaagcgttc ccatgcgcgg
     1561 attgtggcgc gcgctcgggc gggccggagg gcgtggccgt cgtgcacgcg acagggctta
     1621 ttttttgaat gaaatcggcc cctggcgctt ggtggacaag cgcgagcagc tacgtttttt
     1681 atagtccacc cacaacgccc cctacctgtt cgggctgagc ttgtcgaagc ctgctcctct
     1741 gagatccagt ccttcgacaa gctcaggacg aaggatgtga atggcctttg ggtttagcga
     1801 ccaagcactt cagcaaacac cgaggcatca acattggcgc ccgtgagcgc gatgcccacg
     1861 cattgcccgc gcagatttga gcgcccctgc agcgcggcgg ccaacgccgc tgcgccggca
     1921 ccttcggcgg cgttgtgcgt gtcggcatag agggcgcgca tggcgccggc cacttcgtcg
     1981 tcgctcacct gcaccacgtg atccagccgc ccggtcatgg cagccagcgc ggcggcatcg
     2041 gcgcggcgca cggccatgcc atcggcaatc tgcgtcgaca ctggcgcctc gaccacgcgg
     2101 ccggccgcca gcgaatcggc ataagtcgtc gcatgacggc tgaccacgcc gacgatgcgc
     2161 gccttgtggc ccaaggccaa cttcgcggcg atggccgcgc aggcgccgga gccgaggccg
     2221 atgggcacgt aggccacatc cagctgcggc acggccttta agaactccca ccaatacgtc
     2281 gccacgccgc tcaccagatc ggcgtgaaag ctcggcacgc gcagcgcgcc gcgcgcttca
     2341 gccagttgcg cggcgtggtc gctggcttcg gtgaagtcgt cgccgtgctc gatgagatgg
     2401 acgcccagcg cgcgcatggc ggcgttcttc tcgaccgaat tgccgcgcgg cacgacgatg
     2461 gtgcaggcca cgccgtgcgt gcgcgctgcc cagccaatgc tctggccgtg gttgccgcgc
     2521 gtggcgctga cgacttcgcg cggcagctcg ccacggcgcg ccagcgcatc gaaaaaggtc
     2581 agcccgccgc gaatcttgaa cgcgccgacg ggggtgtggt tctcgtgctt gacccagcaa
     2641 tcggtgccca gtcgctccga cagcagcgcc cagcggtatt gcggcgtggg tggaaattcg
     2701 cggtagacga cggcggaagc ggcttcgatc tcgcccagtt tgggcaaggt gctcatgtcg
     2761 gctttttttc gttcaggatg cgcagcgcca gcaagcccag cacgctaccc atcagatagc
     2821 gctgcgcgtt gaccctgcgc gggctgcggt tcaagaagcc cgacacgccg ccggcaaaca
     2881 gcaccagcag cgcgttgatg ccggcgctga ccgctatctg gatcgcgccc agcagcaggc
     2941 tttgcagcag cacgctgccg cgctcggggt gcagaaactg cggaaagaaa gccaggtaga
     3001 acatggcccc tttggggttc agcacgttgg tcatgaagcc catggcaaac agctttttgg
     3061 gcccgtcgtg cggcagcctg cgggcctgaa acggcgccgc gccaccggac cgcaccgcct
     3121 gccacgccat ccacaacagg tacagcgcgc cggcgatgcg gatgacgtca aacgtcatcg
     3181 gcaccgccag cagcagcgcc gtcaggccca gcgcggcggc aaacaggtgc atcagaaagc
     3241 ccagcaccac gccggccagc gacaagaggc cggcgcgcgg cccttgcgtc aggctgcgcg
     3301 agacgcaata gaccatgttg ggcccgggcg tcagcaccag caccagcgcg gcggcggcaa
     3361 acagcagcag ttcgttgagg gtgaacatca gccgccctcc gccgatgaca agaccacctc
     3421 gccacgcggc gtggccagca ccacgtcaat gctggcgtgg acatccggcg cgatggccac
     3481 gtcggccagc ccgaccgctg tgcacgcctc gcccagcgcc gccgcgcgcg ggtggcgcag
     3541 cgtcagcgac cgcagcgtga ggccgctggg cggcatgtcg gtggccgggt gcacgccggc
     3601 ccattcgatc agtgtcggca ggcagccatc gaacaggcgg cggccgtcgg gccgcaccgt
     3661 gatctgccat tcgagcagcc cggcgggcgt catgcggttg gcgcgcagca cctcgccgcg
     3721 gtcgatttcc agcatcgcca gccgcgccgc cgcgtcctgc acgtcgggca cgcgcgccac
     3781 ccagtggatc aggcgcgggc cgtggcggcg caactgcgca cgcaaggctt cgtcgtccat
     3841 gtcaaaccag cgtttcaggc cgccagcgct tgtctggcgt gcgccagcag cttctggatt
     3901 gatagcaatg atctccaggt aggcgagcgg aaacgcatcc gacgccacgg acagcaagcg
     3961 gttgtgggtg ccgaacagcg ggtgctcgcc accggcgccc ggcagcacgc ccagcgtggc
     4021 ttcgcaccac gcggcgccct cggccagcgt gcccgccatc accaccagat ggtcgaggcg
     4081 cgcgcggtcg tgcagggcgg tcacagcgtc acctgaccct ggatcacgcc cacgcagtcg
     4141 ccgccgatcc agacttgtcc ttcggcgtca cgttcgacat gcacccgacc ggcgcggccc
     4201 agcgccgtgc cctgcgcggc gacgtagcgc ggcggcagct cgcccgcctc gatcaaccac
     4261 tgcgccagac tggcgttgag gctgccggtc accgggtctt cgggcacgcc gatgggcgcg
     4321 gcaaaggcgc gcacctcgat cagcggcgct ggtgcgggtg tgccgaatgc gcgcgcctct
     4381 cggctcgacc ttgataccaa ttcgatagct gatggcgctg gatacacgcc gatcacaccg
     4441 actttctgcc ccagcgcggc cagccgtgcg tgatccggct ccagcgccag cacggtgtcg
     4501 gcgctgtcca gctgcagggt cagccagacg gggccgttgt cgagccgctg cgctcgcaaa
     4561 atctgcgccg gctccacgcc cagcgcctgc gcgatgtcgg ccagcagcgc cggcgccggg
     4621 tcgctgcgca cgctgggcgg cgcggcaaag gccagccgtt cgccatcgcg gcgaatcggg
     4681 atcagcccct tggcgcattg ctgcacgatg cggtcggccg acttgggttg cccgcccgct
     4741 gccagccacg cgaaacacga gcccagcgtc gggtggccgg caaacggcag ctcgccgccc
     4801 ggcgtgaaga tgcgcaacgc gtagtcggct tgtgggtcgg tcggcggccg cacgaaggtg
     4861 gtttcggaca aattggtcca gcgtgcaaag cgctgcatgg ccgcgtcgtc gaggcccgca
     4921 ccgtccagca ccacggccac cgggttgccg tagccgggcg cggcggtgaa gacgtcgatc
     4981 tgggcgaagg ggcgcgcgcg catggtcatc cggtcactct caaatcaaga gctgcctgcg
     5041 cttgtttgac aagcgctggc ggcctgattc attcacacat cgcttgttga accacgcgcc
     5101 ccagcgccgc catgcccttg tcgatctgct cgggcgtcga ggtgacaaag gacaggcgca
     5161 gcgcgcgcgc atccggctcg ccggcgtaga acggcgcgcc gggcacgaag gccatgccgg
     5221 catcgacggc cttcggcaac agcgcttgcg cgttcagcct ttcgggcagg cgcacccaga
     5281 cgaacatgcc gccaacgggc tgcgtccact cggcgcccgt ggggcccagt tcgcgcgcca
     5341 gggcggccag catggcgtcg cgctgggcgt ggtagcgggc gcggatggtg ggcacgtgtt
     5401 ggtcgagaaa gccgtccttg atcacctcgg ccaccacgcg ctggttgaag ccgggcgtgt
     5461 gcagatcggc cgcctgcttg gcctgcagca gcttggggta gagcgcgggc ggcgccacga
     5521 tgtagcccag gcgcaggccg ggcgcgagga ttttgctgaa cgagccaagg tacaccacgc
     5581 cttcgggcca gcgcgccagc agcggcttgg gcggcggtgc gtcaaaccac agttcgccat
     5641 acgggttgtc ttcgacgacg ggcagaccgt gttcgcggca gacggcgatc acggcttcgc
     5701 gccgcgcttc gtccatggtg cggccggtcg ggttctggaa gttgggcagc aggtagatga
     5761 agcgaggacg ctcacctttt tgaagcgcct cgcgcaaggc ggatgggcac acgccgcccg
     5821 aatcgctctc aatgctttcc accgcaggct gcatcggcgt gaaggcctgc aaagcgccca
     5881 gataggtggg cgattcgacc agcaggcggc tgtccttgtc gatcagcacc ttggcgatca
     5941 aatccagccc ctgctggctg ccggtggtga tcaacacctg atcgggcgac acggtggcac
     6001 cctgcttgcc cagctcggcg gagacccact ggcgcagctc gggcaggcct tcgctggacg
     6061 cgtactgcag cgacggcttg gcgaccacgc tgccttcggc cagcacgctg gcgcaggcgg
     6121 cgcgcatggc ttcgatgggg aaggtctcgg gcgacggcag gccgccggca aagttgagga
     6181 tgccggggcg ctcggtcacc ttcaggatct cgcggatcgc cgacgggttc atggcgtggg
     6241 cgcgttcggc cagttgccag ggggtgg
//
LOCUS       DAOMVO010000130         6258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3364_length_6258_cov_19.716428, whole genome shotgun sequence.
ACCESSION   DAOMVO010000130 DAOMVO010000000
VERSION     DAOMVO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3364_length_6258_cov_19.716428"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKV30_07615"
     CDS             <1..548
                     /locus_tag="PKV30_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL30116.1"
                     /translation="LVGVLVGQGRQLDHVALVDIVAAGFDAGLRFGESLQADMVALPV
                     GAPQRFCVVASPDYLAHHGAPAEPAQLTAHACINLRFPSGRLYAWQFARGGRAFDVQV
                     QGSFTANDQTALLQAAEAGLGLAYVYAGHAVPLIAQGRLAAVLDDWLPPAEQLYLYYP
                     SRRLQPAGFQALLDAVKAAVD"
     gene            complement(622..1170)
                     /locus_tag="PKV30_07620"
     CDS             complement(622..1170)
                     /locus_tag="PKV30_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ecotin family protein"
                     /protein_id="HRL30117.1"
                     /translation="MSTATWPLSLPTIARLAAPLAAAVLAAACAQSAPPPPAPIPSTP
                     TPAAPGDVLGAFPASMPGHQRHVVKLPPLDNEHLHRVELLGGKTLKVDCNRQSMDGHF
                     SPREVPGRNYPYWVFSSKGQVMSTRMGCPPGSDRQQFVSAETLLVPYNSRIPLVVFVP
                     EGFEFRSRPFDTRAAGLPAPAR"
     gene            1645..2841
                     /locus_tag="PKV30_07625"
     CDS             1645..2841
                     /locus_tag="PKV30_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30118.1"
                     /translation="MTPAERRASLSLAGIFALRMLGLFLVLPVFALEAARLPGGNDPA
                     LVGLAMGIYGLTQAVMQVPLGLASDRVGRKKIIVIGLIVFALGSVVAALAGGVQMLIV
                     GRAIQGAGAVSAAVTALLADLTRDEVRTKGMALVGISIAAMFALSLVAAPPLAGAIGL
                     GGLFWITAALALAGIAVVLWWTPAEPVQHTDVPRGSLADVWLHPDLWRLDLGVFVLHT
                     VQMAMWVVVPGLLVQAGLPKSQHWHVYLPAVLASFLMLGGLFAAERRGQLRAVLRIGI
                     ALLFVVQVALWLVAPRAPGLWTLGTLMFFFFIAFNLLEATQPSLISRLAPAHARGAAL
                     GVYNTLQSLGLFAGGALGGWLVKAGGPQAVLVATSALALLWLLAGWAQVVPAATQPSA
                     ARASAP"
     gene            complement(2873..4156)
                     /locus_tag="PKV30_07630"
                     /pseudo
     CDS             complement(2873..4156)
                     /locus_tag="PKV30_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            4251..4775
                     /gene="ssb"
                     /locus_tag="PKV30_07635"
     CDS             4251..4775
                     /gene="ssb"
                     /locus_tag="PKV30_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRL30119.1"
                     /translation="MASVNKVILVGNLGRDPEMRSFPSGDQVANVTLATTDKWKDKQS
                     GEPREHTEWHRLVFNGRLAEIAGQYLRKGSQIYVEGSIRTRKWQDQATGQDRYATEVR
                     VDQMQMLGSRQGMGGPSDDGGGGYGGGGDGGGYDAPRRAAPAPRAAPAPRAPAPAASK
                     PGSGFDDMDDDIPF"
     gene            4984..5877
                     /locus_tag="PKV30_07640"
     CDS             4984..5877
                     /locus_tag="PKV30_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL30120.1"
                     /translation="MTHTVSRRHLLGAAAFAPLVARAQDTAPAPIFGQPGKDVIWAPT
                     PDAMVDRMLRLAGLRPRDFVIDLGAGDGKIVIAAAKAGARGLGIEYNPELVTLARQRA
                     QAAGVAEHARFEKADIFESDFSRASIVTLYLLPRLNLRLRHRLLALTPGTRVVSHSFD
                     MGDWRPDEVTNAGGATAFLWLVPANFGGEWALRFPLADGSEARGLLEVDQRFQQIGGA
                     AVFRDFTASLRQPLLAGDRVRFALTDAEGQLREFDGRIAGRELIGTVTQGDARVPFRA
                     DRTDAAIPPIGGSEPVRPPRD"
     gene            complement(5957..>6258)
                     /locus_tag="PKV30_07645"
     CDS             complement(5957..>6258)
                     /locus_tag="PKV30_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRL30121.1"
                     /translation="LVVVNAGPGGKDDPGVVDGYEGERLAERLAKLPVPAEFKHFVRG
                     KSTVDEIEALVNHLDVSVLVIGLRRRTAVGKLLLGSTAQEILMTVSCPVLCVKAA"
BASE COUNT          945 a         2199 c         2191 g          923 t
ORIGIN      
        1 agcttgtggg cgtcctcgtc gggcagggcc ggcagctcga tcacgtggcg ctggtcgaca
       61 tcgtggccgc cggcttcgat gccgggctgc gttttggcga aagcctgcag gccgacatgg
      121 tggcgctacc ggtgggcgcg ccgcagcgct tctgcgtcgt ggcgtcgccg gactacctgg
      181 cgcatcacgg cgcgcccgcc gagccggcgc agctgacggc acacgcgtgc atcaacctgc
      241 gctttccgag cgggcgcctg tacgcgtggc agtttgcgcg cggcggtcgc gcgttcgacg
      301 tgcaggtgca gggcagcttc accgccaacg accagacggc gctgctgcag gcggccgagg
      361 ccgggctggg cctggcctac gtctacgccg gccacgccgt gccgctgatc gcgcaggggc
      421 gcctcgccgc cgtgctggac gactggctgc cgccggccga gcagctgtac ctgtactacc
      481 cgagccggcg cctgcagccg gcggggtttc aggcgctgct ggatgcggtc aaggccgctg
      541 tcgactgacg ggttcttgaa gaaaaaggcc accagcgctt gatggacaag cgcaagcagc
      601 tatcaaaata aaagcgtcga gtcaacgcgc cggcgccggc agaccggcgg cgcgggtgtc
      661 gaacgggcgc gagcggaact cgaagccctc cggcacgaac accaccagcg ggatgcggct
      721 gttgtagggc acgagcagcg tttcggcgct gacgaactgc tggcggtcgc tgcccggcgg
      781 gcagcccatg cgcgtgctca tgacctgacc cttgctgctg aacacccaat acgggtagtt
      841 gcggccgggc acctcgcgcg gcgagaagtg gccgtccatg ctctggcggt tgcagtccac
      901 cttcagcgtc ttgccgccca gcaactcgac gcggtgcagg tgctcgttgt ccagcggtgg
      961 cagcttgacc acgtggcgct ggtggccggg catgctggcc ggaaaggcgc cgagcacgtc
     1021 gcctggcgcg gcgggcgtgg gcgtcgaggg gatcggcgcg ggcggcggcg gtgccgactg
     1081 cgcgcacgcc gccgccagca cggcggcggc cagcggcgcg gccaggcgag caatggtggg
     1141 caggctgagc ggccaggtgg ctgtggacat ggaaaagacc tttcgtattc gtttgcacag
     1201 ccagcaagcc taagccggcg acggcgcgag tggcgcgcac gggcgccggg cacgcgtgtc
     1261 ggccgatgcg tacaaggggc ctgaagtcgg cccgtggcgc gcggtttcaa aggtggtggc
     1321 acagcggcat ggtggctgtg ggagcggcct tggccgcgat gccggccgca cggtggcgca
     1381 acagcctgat cgcggccacc gccgctccac ggtgacggcc tgaaacagag accaagcggt
     1441 taaaaacgcc tccagcgctt gccaatcaag cgcaagcagc tatcaaaatc agagtcttcg
     1501 tgatgccccc gatcgggtgc agacggatca ccgacaatag ccgcgtcgcg ggctggcccc
     1561 gccgcccacc gcatcgcgat cccgatgtct gccatttccg acgcgccccg ctccccccct
     1621 tccgctcccg ccgccacaca ccgcatgacg ccggccgagc gccgcgccag cctgtcgctg
     1681 gcgggcatct tcgcgctgcg catgctgggc ctgttcctgg tgctgccggt gttcgcgctg
     1741 gaggcggcgc gcctgcccgg cggcaacgac ccggcgctgg tcggcctggc catgggcatc
     1801 tacggcctga cgcaggcggt gatgcaggtg ccgctggggc tggcgtcgga ccgcgtgggg
     1861 cgcaagaaga tcatcgtcat cggtctcatc gtctttgcgc tcggcagcgt ggtggcggcg
     1921 ctggcgggcg gtgtgcagat gctgatcgtc ggccgcgcca tccagggcgc gggcgcggtg
     1981 tcggccgccg tcacggcgct gctggccgac ctgacgcgcg acgaagtgcg caccaaaggc
     2041 atggcgctgg tgggcatcag catcgcggcg atgtttgcgc tgtcgctggt ggccgcgccg
     2101 ccgctggccg gggcgattgg gctgggcggc ttgttctgga tcaccgccgc gttggcgctg
     2161 gccggcatcg ccgtggtgct gtggtggacg ccggccgagc ccgtgcaaca caccgacgtg
     2221 ccgcgcggca gcctggccga cgtatggctg catcccgacc tgtggcgact ggatctgggc
     2281 gtgttcgtgc tgcacacggt gcagatggcg atgtgggtgg tggtgccggg gctgctggtg
     2341 caggcggggc taccaaaatc gcagcactgg catgtgtatc tgccggcggt gctggcttct
     2401 ttcctgatgc tgggcggctt gttcgcggcc gagcggcgcg gccagttgcg cgccgtgctg
     2461 cgcatcggca tcgcgctctt gtttgtggtt caggtcgcgt tgtggctcgt ggcgccgcgc
     2521 gcaccggggc tgtggacgct gggcacgctg atgttcttct tcttcatcgc cttcaatttg
     2581 ctggaagcca cgcagcccag cctgatctcg cgcctggcgc cggcccacgc gcgcggcgcg
     2641 gcgctggggg tttacaacac gctgcaatcg ctggggctgt ttgccggcgg cgctttaggc
     2701 ggctggctgg tcaaggccgg ggggccgcag gcggtgctgg tggcgacgtc ggcgctggcg
     2761 ctgctgtggc tgctggcggg ctgggcgcag gtggtgccgg ctgcgactca gccatctgct
     2821 gctcgggcat ccgcgccgta gcatccggcg ccagccgcgc caccagctgg tcggcaatcg
     2881 tggcgtacag cggccggctg accatgcgcg acaccagact ggccagcagc gcgcacgaca
     2941 tcagccccag cagcatggcg tagccgtcga ccatctccat cacgatgatc atggcggtga
     3001 tcggcgtttg cgtcatcgcg gccagaaagc ccgccatgcc cagcgcgatc agcacggtgg
     3061 cgtggtcggg cgccagcagc agcgccacgt cgtgccccag gctggcgccg atcgacaggc
     3121 tgggcgtgaa cagcccgccc ggcgcgcccg accaggccga cagccaggtg gcgacgaatt
     3181 tcagcagtgt ccacagcagc ggcacgctgg ccccgtcgcc cgccagcagc gtgcgcgtgt
     3241 gcgcgtggcc gccgcccacg gcgctggcct gcgtcaggat gccgatcacc gccaccgcca
     3301 gcccacaggc ggcggcaaag cggatcgggt gcttgcggcg aaaggcgctg aaacgatccc
     3361 gaccgccaaa cagcgacgtg atcaagagcc gcgcaaacag cccgccgatc aagccacaga
     3421 ccagcgccag cgccagcccc ggcagcagca cgccccagcc cacgcgctcg atgcggatgc
     3481 ggccgaagta cgacaggttg ccgaacgccg ccaccgccac caggccggcc atcacgatgg
     3541 cgcccagcat caggccggac gagcgcgtct ccagccggcg cgacagctcc tcgatggcaa
     3601 acacgatgcc gcccagcggt gtgttgaaag ccgccgcaat gccggccgcg ctgccggcca
     3661 ccagcagctc gcgcgcgctg atgcccgaac gcggcgacag aaagcgccgc gccgcgtaca
     3721 tcacgccggc ggcgatctgc accgacgggc cttgccggcc aatcgacagc cccgccagca
     3781 gcccgccaaa cacggccagc gccttggcca gcgaaatctt cagcgacacc agcgccgagc
     3841 gcgaaggcgc gtccagccgc ggatcgagcg ccgccagcgc ctgcggcggc cccgagccgc
     3901 cggcgccggg cgcaaagcgc atcgtcgccc acaccaccaa cgccgtcagc gccggcgtcc
     3961 acagcagcgg ccagaagcgc cattcgccgg acacggcctt gaaaatctgc tcgcccgcgt
     4021 cggccagcag cgtgaagacc accaccgaca gccccgccag cagcgcgaac aggccaacca
     4081 ccgcgcggtc gatccacacg cgcgggtcga tcacctcgct gcgcacctgg cccaggaaat
     4141 ccgggtcgtc cttcatgcgc ggcattctgg catgcgtgcc ggggggcttg cggcacaatg
     4201 gcgggttccg ttcaaccgct tgttcacctt cacctttaca ggaactcgcc atggcctccg
     4261 tcaacaaagt catcctcgtc ggcaacctgg ggcgcgaccc cgagatgcgc tctttcccca
     4321 gcggcgacca ggtcgccaac gtcacgctgg ccaccaccga caagtggaaa gacaagcaaa
     4381 gcggcgagcc gcgcgagcac accgaatggc accgcctggt cttcaatggc cgcttggctg
     4441 aaatcgccgg gcaatacctg cgcaaaggca gccagatcta cgtcgaaggc agcatccgca
     4501 cccgcaaatg gcaagaccag gcgaccggcc aggaccgcta cgccaccgaa gtgcgcgtcg
     4561 accagatgca gatgctgggc agccgccagg gcatgggcgg gccgtcggac gatggcggtg
     4621 gcggctacgg cggtggtggt gatggcggtg gctacgacgc cccacgccgc gcggcgcccg
     4681 ccccgcgcgc ggcccctgca ccgcgcgcac cggcgcctgc cgcgtcaaag cccggctcgg
     4741 ggtttgacga catggacgac gacatcccgt tctgacgctc cccctgaggc gctgacgcgc
     4801 cttccccctg tgggggacaa cgctggcggc cggggggacc ccggccacgg cgttccggca
     4861 tggcctgctc cgcggccttc tgagcggctt gctgcgcagc ctgggatgaa gtgggcgatc
     4921 atgtttttga tgaaacaggc ctccagcgct tgtctatcaa gcgcgaacag ctatcatttt
     4981 gatatgaccc acaccgtctc ccgccggcac ttgctggggg ccgccgcatt cgccccactc
     5041 gtggcgcgag cgcaggacac ggcgccagcc cccatctttg gccagcccgg caaggacgtg
     5101 atctgggcgc ccacacccga tgccatggtc gatcgcatgc tgcggctggc gggtctcagg
     5161 ccgcgcgatt tcgtgattga cctgggcgcg ggcgacggca agatcgtcat cgccgccgcc
     5221 aaggccggtg cgcgcgggct gggcattgaa tacaaccccg agctggtgac cttggcgcgg
     5281 cagcgcgcgc aggcggccgg cgtggccgag cacgcgcgct tcgaaaaggc cgacatcttt
     5341 gaatccgact tctcgcgcgc cagcatcgtc acgctgtacc tgctgccgcg cctgaacctg
     5401 cgcctgcgcc atcgcctgct ggcgctcacg ccgggcacgc gcgtggtgtc gcactcgttc
     5461 gacatgggcg actggcggcc cgacgaagtc accaacgcgg gcggggcgac ggcctttttg
     5521 tggctggtgc cggccaactt tggcggtgaa tgggcgctgc gctttccgct cgccgatggc
     5581 agcgaggcgc gcggtttgct ggaggtcgac cagcgctttc agcaaatcgg cggcgccgcc
     5641 gtgttccgcg atttcaccgc cagcctgcgc cagccgctgc tggccggcga ccgcgtgcgc
     5701 tttgcgctga ccgatgccga gggccagctg cgcgagttcg acggccgcat cgccgggcgc
     5761 gagctgatcg gcacggtgac gcaaggcgat gcgcgcgtgc cgtttcgcgc cgaccgcacc
     5821 gatgcggcca tcccacccat cggcggcagc gagccggtgc ggccgccccg cgattgattt
     5881 tcaagccaaa tcggcccctg gcgcttgtgt atcaagcgcc agcagctatc aaaaacagag
     5941 tattcgcgga taggccctag gccgctttca cgcacagcac ggggcacgac accgtcatca
     6001 gaatctcctg cgccgtgctg cccagcagca gcttgcccac ggcggtgcgg cggcgcaggc
     6061 cgatcaccag caccgacacg tccaggtggt tgaccaacgc ctcgatctcg tcgaccgtgc
     6121 tcttgccgcg cacgaagtgc ttgaactcgg cgggcaccgg cagcttggcc aggcgctcgg
     6181 ccagacgctc gccctcgtag ccgtcgacga cgccggggtc gtccttgccg cccgggccgg
     6241 cgttgaccac caccagcg
//
LOCUS       DAOMVO010000131         6255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3366_length_6255_cov_13.942581, whole genome shotgun sequence.
ACCESSION   DAOMVO010000131 DAOMVO010000000
VERSION     DAOMVO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_6255_cov_13.942581"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKV30_07650"
     CDS             complement(<1..368)
                     /locus_tag="PKV30_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRL30122.1"
                     /translation="MWHPGTLQPIGPDDLAPLFPMSLIEQEVTTEREVEIPEPVREVF
                     RLWRPAPLYRAHRLEKVLGTPAKIFYKYEGVSPAGSHKPTSAIPQAFYNAQAGIKRLT
                     TETGAGQWGSSLAMAGQLFGL"
     gene            527..1045
                     /locus_tag="PKV30_07655"
     CDS             527..1045
                     /locus_tag="PKV30_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HRL30123.1"
                     /translation="MRISLSWRRHLGTLAMVAVALLGAHAWQTRQVPSGPAPDFSALA
                     VSTQDGAVLGLAQWRAAHPGQPVALHFWAEWCAICKLEQHSVTRVASDWPVLTVAMQS
                     GDVAAVRRTLAQRGLNWPTVIDADGALAARYGFRAVPAFVAIDPAGRISATSVGYTSE
                     IGMRLRLWWARL"
     gene            complement(1135..2583)
                     /gene="sbcB"
                     /locus_tag="PKV30_07660"
     CDS             complement(1135..2583)
                     /gene="sbcB"
                     /locus_tag="PKV30_07660"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HRL30124.1"
                     /translation="MAAPTFLWHDYETFGAVPRRDRPAQFAAIRTDADLNEIGEPMML
                     YCQPALDVLPEPESCLITGITPQECLAKGVPEHEFARRINDALAEPGTIGVGYNSIRF
                     DDEVTRHLLWRNLRDPYAREWQNGCGRWDLLDVVRTAHALRPGGIEWPKGDDGHTTFR
                     LERLSAANGLVHESAHDALSDVRATIALARLVKTTNPKLFDFCLALHKKERVAQELRL
                     PTTLQDARPFLHISGMFGAARGCLAVMAPLAMHPTNKNELLAWDLSADPAELADLKPE
                     AIRERLFTPTADLPEGVTRLPVKGVHLNKSPMVIGNLKTLTDAQAERYGIDLAQVERH
                     FERLRALPDMSALWPQVYARPPAGTPVDVDEDLYGGFVGNADRRRLNDVLRLSPAELS
                     LARTGFDDARLGELVWRYRARNFPDTLNETDQARWAEHRAGRLIRGDAGALTLQAFFD
                     RIDGLAESADDERAQGVLEALYEWGEHIAPEV"
     gene            complement(2615..3502)
                     /locus_tag="PKV30_07665"
     CDS             complement(2615..3502)
                     /locus_tag="PKV30_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30125.1"
                     /translation="MAAIDTLDWRLLQVFLVAAEAGSLGRAAAALGDSQPTLSRRLAE
                     LEAALGQALFERSPRGLSPTAAGSALLGPARRMQQQTRQMALAMERHAGTLAGTVRVT
                     ASEMVSQHLLLPALRPLREQHPEIQIELAPGDGVADLLRREADVAVRMFRPREPALIA
                     RRLADLPLGLYAHRDYLARHGKVMPASLAQHHWIGADKGDETRRGFAQAGHPVAREFF
                     AIRTDHSALAWQAVCEGLGIGVGLRQVAERNADLVRVLAGIAIPPMACWLVVHRELRG
                     TPRLRAVADALAAALGRAA"
     gene            3607..4575
                     /locus_tag="PKV30_07670"
     CDS             3607..4575
                     /locus_tag="PKV30_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRL30126.1"
                     /translation="MSSARPPLHFILGDGVIGQAVADELARLGQLLTLASRSGTKGAS
                     PHALTRVDALDAAALRAATRGCSHLYLTLGLPYVTAVWQRDWPRVMQATIDAALAHDA
                     VLVFFDNVYAYGPLPLRVPMREDHPLQPPSCKGAVRASLGRLLARAQHERGLKWVIGR
                     SADFYGPGVRQSALFVAAIERQLNGHAAQWLGHPDARHSFTYTLDAARGLVRLALDED
                     AWHRAWHLPTTAPAPTPRQLLMQSARLLHAPERIQVLPSALLGVLKVFMPTLREYSEM
                     LYQNRQDYVFSSEAFMARYPDFVVTSYEEGVARMVQSLQSPSKKPA"
     gene            complement(4679..>6255)
                     /locus_tag="PKV30_07675"
     CDS             complement(4679..>6255)
                     /locus_tag="PKV30_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL30127.1"
                     /translation="YALDMGALLAGTKYRGDFEQRLKGVLKNLKDKPGAILFIDEIHT
                     LIGAGAASGGTLDASNLLKPALSSGQLKCIGATTFTEYRGIFEKDAALSRRFQKIDVV
                     EPTVPETVEILKGLKSRFEEHHSVKYATAALQAAAELSAKYITDRHLPDKAIDVIDEA
                     GAAQRIALASKRKKTIGKHEIEEIVAKIARIPPASVSNDDRSKLQTLERDLKSVVFGQ
                     DKALDVLAAAVKMARSGLGKADKPIGSFLFSGPTGVGKTEAAKQLAYIMGVELIRFDM
                     SEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVSKKPHSVLLLDEIEKAHPDIFNVLLQ
                     VMDHGTLTDNNGRKADFRNVIIIMTTNAGAETLNKSSIGFTNARQAGDEMADIKRLFT
                     PEFRNRLDAIVSFKPLDENVILRVVDKFLLQLETQLVEKKVEVTFNDTLRKYLAKKGF
                     DPLMGARPMQRLIQDTIRKALADELLFGRLTEGGRLTVDLDDKDEVLLDITPTPKRER
                     ARGAEPAEPEEEVAAD"
BASE COUNT          955 a         2092 c         2119 g         1089 t
ORIGIN      
        1 agcccgaaca actggcccgc catggcgagc gatgagcccc actgcccggc gccggtttcg
       61 gtggtcaggc gcttgatgcc ggcctgcgcg ttgtagaagg cctgcggaat ggccgaggtg
      121 ggcttgtggc tgccggcggg gctgacgcct tcgtacttgt agaaaatctt ggccggcgtg
      181 cccagcacct tctccagtcg atgggcgcgg tacaacggcg cggggcgcca caggcggaac
      241 acttcgcgca ccggctcggg gatctcaacc tcgcgctcgg tggtcacttc ctgttcgatc
      301 aggctcatcg gaaacagcgg cgccaggtcg tccgggccaa tcggctgcag ggtgcccggg
      361 tgccacaccg gcggcagggc ggccggcaga tcggcctgaa tgttttacca gaacttgggc
      421 aactggcttt cctgcagcaa gtacttggtc tgtgtgctca tggggggtgt ctcctccggt
      481 gtggtctaaa gtcgagcagt atgcctgcgg tcaaacacgg acttccatgc ggatttcact
      541 gagctggcgc cgtcacctcg gcaccttggc gatggtggcg gttgcgctgc tgggcgcgca
      601 cgcctggcag acgcgccagg tgccgagcgg gccggcgccc gatttcagcg cgctggccgt
      661 cagcacgcag gacggcgcgg tgctcggcct ggcgcagtgg cgcgccgccc acccggggca
      721 gccggtggcg ctgcatttct gggccgagtg gtgcgcgatc tgcaagctgg agcagcacag
      781 cgtcacccgc gtggcaagcg actggccggt gctgacggtg gccatgcagt cgggcgacgt
      841 ggcggcggtg cgccgcaccc tggcgcagcg cgggctgaac tggcccacgg tgattgacgc
      901 cgatggcgca ctcgccgcgc gctacggttt tcgcgccgtg cctgcgtttg tcgccatcga
      961 tccggcgggc cgcatcagcg cgaccagcgt gggctacacc agcgagatcg gcatgcggct
     1021 gcggctgtgg tgggcaaggc tttgacgcag gtgcgattca agccgaaaat gtgaaccaaa
     1081 agtgccgcca gcgcttgatt gacaagcgcg agcagctatc ttttcaatag ttcttcagac
     1141 ttcgggggcg atgtgttcgc cccattcgta caaggcttcc agcacgcctt gcgcgcgctc
     1201 atcgtcggcc gattcagcca agccgtcgat gcggtcgaag aaggcctgca gcgtcagcgc
     1261 cccggcatcg ccgcggatca accgacccgc gcggtgctcg gcccagcgcg cctgatctgt
     1321 ctcgttcaga gtgtcgggga agttgcgtgc ccggtagcgc cacaccagct cacccagccg
     1381 cgcatcgtca aaaccggtgc gcgccagcga aagttcagcc ggcgagaggc gcagcacgtc
     1441 gttcaggcgg cgccgatctg cattgccgac gaagccgccg tacaggtcct cgtcgacatc
     1501 caccggcgtg ccagcgggcg ggcgcgcata cacctgcggc caaagcgcgc tcatatcggg
     1561 cagcgcgcgc aggcgctcga agtggcgctc gacttgcgcc agatcgatgc cgtaacgctc
     1621 ggcctgggcg tcggtcaagg tcttgaggtt gccgatgacc atgggcgact tgttcaggtg
     1681 cacgcccttg acgggcagcc gcgtcacgcc ttcgggcaaa tccgccgtgg gggtgaacag
     1741 ccgctcgcgg atcgcctcgg gcttcaggtc agccagctcg gccgggtcgg ccgacaagtc
     1801 ccaggccagc aattcattct tgttggtcgg gtgcatggcc aacggcgcca tcaccgccag
     1861 gcagccgcgc gcagccccga acatgccgct gatgtgcaga aagggccgtg cgtcttgcag
     1921 cgtggtcggc aggcgcagct cttgcgccac gcgctccttc ttgtgcagcg ccaggcagaa
     1981 gtcaaacagc ttggggttgg ttgttttgac cagccgcgcc agcgcaatgg tggcgcgcac
     2041 gtccgacagc gcgtcgtgcg ccgactcgtg caccaggccg ttggcggcgc tcaagcgttc
     2101 gagccggaag gtggtatggc catcgtcgcc cttcggccac tcgatgccgc ccggccgcag
     2161 cgcgtgggcg gtgcgcacca cgtcgagcag atcccagcgg ccgcaaccgt tttgccactc
     2221 gcgcgcgtag gggtcgcgca ggttgcgcca cagcaggtgg cgcgttacct catcgtcaaa
     2281 gcggatcgag ttgtagccga cgccgatggt gccaggctca gccagcgcgt cgttgatgcg
     2341 gcgcgcaaac tcgtgttccg gcacgccttt ggccaggcat tcctgcggcg tgatgccggt
     2401 gatgaggcag ctttcgggct cgggcagcac atccagcgcc ggctggcagt acagcatcat
     2461 cggctcgccg atctcgttca agtcggcgtc ggtgcggatg gccgcgaact gcgccggccg
     2521 gtcgcgccgc ggcacggcgc caaaggtttc gtagtcgtgc cagagaaagg tgggggcggc
     2581 catggcgtca agcataacca gcgcgacgtc accctcaggc ggctcggccc agcgctgccg
     2641 ccaacgcgtc ggccaccgcg cgcagccgtg gcgtgcctcg cagctcgcgg tgcaccacca
     2701 gccagcaggc catgggcggg atggcgatgc cggccagcac gcgcaccagg tcggcgttgc
     2761 gctcggccac ctgccgcagg ccgacgccga tgcccagccc ctcgcacacg gcctgccagg
     2821 ccagagctga atgatcggtg cggatggcga aaaattcacg cgccaccggg tgaccggcct
     2881 gcgcaaagcc gcgccgcgtc tcatcgccct tgtcggcgcc gatccagtgg tgctgcgcaa
     2941 gcgacgctgg catcactttg ccgtggcgcg ccagatagtc gcggtgcgcg tacaggccca
     3001 gcggaagatc ggccaggcgg cgcgcgatca gcgccggctc gcgcgggcgg aacatgcgca
     3061 cggcgacgtc ggcttcgcgc cgcagcagat cggccacgcc gtcgccgggt gccagctcga
     3121 tctggatctc cgggtgctgc tcgcgcagcg gccgcaacgc gggcagcagc aggtgctggc
     3181 tcaccatctc gctggcggtg acgcgcaccg tgccggccag ggtgccggcg tggcgctcca
     3241 tggccagcgc catctggcgt gtctgctgct gcatgcggcg cgccgggccg agcagcgcgc
     3301 tgccggccgc cgtcggcgac aggccgcgcg gcgaccgctc aaacagcgcc tgaccgagtg
     3361 ccgcctccaa ttcggccagc cgtcggctca gggtgggctg gctgtcgccc agcgccgccg
     3421 cggcgcggcc cagcgagccg gcttcggcgg ccaccaaaaa aacctgcagc aggcgccagt
     3481 ccagggtgtc gatcgcggcc atgcgtcatg cactggtgaa tgagggtcat gccatggtag
     3541 tgcttcccga acgtcgtggg ctgcaccacc atggcgccac ttcgttgtca attaaaggag
     3601 gcacgcatgt catccgcccg gccacccctt cacttcatcc tcggcgacgg cgtcatcggc
     3661 caggccgtgg ccgatgaact ggcgcgcctg ggtcagctgc tcacgctggc cagccgcagc
     3721 ggcacaaaag gcgcttcacc acacgccctt acacgggtcg atgcgctgga cgccgccgcc
     3781 ttgcgcgcgg ccacgcgcgg ctgcagccat ctctacctga cgcttggcct gccctacgtc
     3841 accgccgtgt ggcagcgcga ctggccgcgc gtgatgcagg ccaccatcga cgccgctctg
     3901 gcccacgacg cggtgctggt gttcttcgac aacgtgtacg cctacgggcc gctgccgctc
     3961 agggtgccga tgcgcgagga tcatccgctg cagccgccat cgtgcaaggg cgccgtgcgc
     4021 gccagcctgg gccgcctgct ggcgcgggcg caacacgaac gcgggctgaa atgggtgatc
     4081 gggcgcagtg ccgatttcta cgggccgggc gtgcgccagt cggcgctgtt tgtcgccgcc
     4141 atcgaacgac agctgaacgg ccacgcggcg caatggctgg gccacccgga tgcgcgccac
     4201 agcttcacct acaccctgga cgctgcgcgc ggcttggtgc gcctggcctt ggacgaggat
     4261 gcttggcatc gcgcctggca cctgcccacc accgcaccgg caccgacgcc gcgccagttg
     4321 ctcatgcaat cggcgcgcct gctacacgcg cccgagcgca ttcaggtgct gccatcggcg
     4381 ctgctcggcg tgctcaaggt cttcatgccc accctgcgtg aatacagcga aatgctgtac
     4441 cagaaccggc aggactatgt gttcagcagc gaggccttca tggcacgcta cccggatttt
     4501 gtcgtcacgt cgtatgaaga aggtgtggcg cgcatggtgc aatcactgca atcgccatca
     4561 aaaaagcccg cgtgacagcg ggcctcgtga acaggagttt ttgaagaaat caggctccag
     4621 cgcttgtcca tcaagcgcgg caagctacaa ataaagtagc ttgccgcctc atgatggatc
     4681 agtcggccgc gacctcttcc tcaggttcgg ccggctcggc accccgcgcg cgctcgcgct
     4741 tgggcgtggg cgtgatgtcg agcaacacct cgtccttgtc gtccagatcc accgtcaggc
     4801 ggccaccttc ggtcaggcga ccgaacagca gctcgtcggc cagcgccttg cggatcgtgt
     4861 cctgaatcag gcgctgcatc gggcgcgcgc ccatcagcgg atcgaagccc ttcttggcca
     4921 gatacttgcg cagcgtgtcg ttgaaggtga cttcgacctt cttctcgacc agttgcgttt
     4981 ccagctgcag caggaacttg tcgaccacgc gcaggatgac gttttcatca agcggcttga
     5041 agctgacgat ggcgtcgagc cgattgcgga actccggggt gaacaggcgc ttgatgtcgg
     5101 ccatctcgtc gcccgcctgg cgcgcgttgg tgaagccgat gctgctcttg ttcagcgttt
     5161 cggcacccgc attcgtcgtc atgatgatga tgacgttgcg gaagtcggcc ttgcgcccgt
     5221 tgttgtcggt cagcgtgccg tggtccatca cctgcagcag cacgttgaag atgtccgggt
     5281 gcgccttctc gatttcgtcc agcagcagca cgctgtgcgg cttcttgctg acggcctcgg
     5341 tgagcaaacc gccctggtcg aagccgacgt agccgggagg cgcgccgatc aggcggctca
     5401 cggcatggcg ctccatgtac tccgacatgt cgaagcggat caactcgacg cccatgatgt
     5461 aggcgagttg cttggccgcc tcggtcttac caacgccggt gggacccgag aacaagaagc
     5521 tgccaatcgg cttgtcggcc ttgccgagcc ctgagcgcgc catcttgacg gcggcagcca
     5581 gcacgtcgag ggccttgtct tggccgaaca ccacgctttt caggtcgcgc tccagcgtct
     5641 gcagcttgct gcggtcgtcg ttgctcacgc tcgcgggcgg aatgcgcgcg atcttggcca
     5701 cgatctcttc gatctcgtgc ttgccgatgg tcttcttgcg cttgctcgcc agggcgatgc
     5761 gctgcgccgc gccggcctcg tcgatgacgt cgatggcctt gtcgggcagg tgacggtcgg
     5821 tgatgtactt ggccgacagc tcggcggcgg cctgcagggc ggcggtggcg tacttcacgc
     5881 tgtggtgctc ctcgaagcgg ctcttgaggc ccttgaggat ttccaccgtc tcgggcaccg
     5941 tcggctcgac gacgtcgatc ttctggaagc gccgcgacag cgccgcgtcc ttctcgaaga
     6001 tgccgcggta ttcggtgaac gtggtcgcgc cgatgcactt gagctggccg ctggagagcg
     6061 cgggcttgag cagattggac gcgtccagcg tgccgcccga cgccgcgccg gcgccgatca
     6121 gggtgtggat ttcgtcgatg aacaggatgg cgcccggctt gtccttgagg tttttcagca
     6181 cgcctttcag ccgctgctca aagtcgccgc ggtacttggt gccggccagc agcgcgccca
     6241 tgtcgagcgc gtaga
//
LOCUS       DAOMVO010000132         6213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3390_length_6213_cov_12.536375, whole genome shotgun sequence.
ACCESSION   DAOMVO010000132 DAOMVO010000000
VERSION     DAOMVO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_6213_cov_12.536375"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            624..863
                     /locus_tag="PKV30_07680"
     CDS             624..863
                     /locus_tag="PKV30_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30128.1"
                     /translation="MQADGQPCHHGPLRLLTGPRRIEVGWWPDAAEAASKPAARDYFI
                     AQTAAAELLWIYRERATAAQPSDAAPRWFLQGLYA"
     gene            856..4602
                     /locus_tag="PKV30_07685"
     CDS             856..4602
                     /locus_tag="PKV30_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRL30129.1"
                     /translation="MPELSRKEEARRAEAARRKNNVVALPRRSPRTRQGGDRPDYAEL
                     HCLTHFSFQRGASSPEDLVRRAWALGYTALAITDECSVAGVVRAWKERNDSQKALQAG
                     EADDDLQRLHASRTALGAHEPLKLLVGSEFRFEGQGTLVVLARDLYGWGNLCEFITHA
                     RRAAPKGGYRLPPLADALVALQGCELLWSPMRGTLDTPESIASCADWISAAGRFDSKI
                     TLLGELNGAADDALWLSQLQHLKEATGCSLAAAGDVHLHVRAAKRLHDVMTAIRIGKP
                     VAECGFELHANGERHLRSRERLAEVVPPDLLAATLAVAQRCTFTLEEVKYNYPSEALI
                     EGLTATETLRRVTYAGAAERYPHGVPPDIERLIEKELALIAECRYEMFFLTVHHIVCF
                     AKSQGILCQGRGSAANSVVCWCLGITAADPEKSSPLLERFISKARQNEPPDIDVDFEH
                     ERREEVIQYIYKEYGRHRAALAATVICYRTRSALRDVGKALGVDERLIDAFAKDHHWF
                     DEGLAVHRLGELAEGVGASLTPRLAALWLELTEQLRGAPRHLSQHVGGFVLTETPLHR
                     LVPLEPAAMEGNAEWKASAHEEPRAPAFTSAAMAQVAQGLPPLPALRQVIQWDKDDLE
                     ALSLMKVDVLALGMLTAMRRCIDMVERRRGEPFGRSDIQSECPRTYEMIRRADTVGTF
                     QIESRAQMSMLPRLKPTTFYDLVVEVAIVRPGPISGGMVHPYLKARERARRGLPIEYE
                     RSKHDMPGQKPRLAKALGRTLGIPIFQEQVMELAMIAADYTAEGADRLRRDMAAWKKK
                     GGLEPHRDKLMAGMLRNGYQRAFAERLFDQIKGFGEYGFPESHAYSFALIAYESSWLK
                     CHEPEAFVAAMLNSQPMGFYGPAQLVQDAKRHGVVVLPPDVTMSDWDCALEEESGSEA
                     KKMAEKMVEKMGSDQTYFESNPPFPLVNQTQAAINSGAFTSSAALRERVGVRGLFAPS
                     PSGRGLGRGQTTFVASAADPHPSLPPAGEGETCRPAVRLGLRLVQGLNEGAARRIEAA
                     RADQPFASIDDLARRAQLDTRQLNQLAAADALLSLAGHRRQQVWQSSALKAAPEFLRD
                     APIDEAPLELAAAPEGEEIVHDYASIGLTLRRHPVALLRPRLQRWRLKSAEELKAMPH
                     GQQVRACGIVIGRQQPGTAKGTIFVTLEDETGSVNVIVWKSVRVDEDQRNALLRSRLL
                     AVQGEWQRDKDSGGHVRHLIARRFVDLTPLLGRIAGLTTSRDFH"
     gene            4760..5188
                     /locus_tag="PKV30_07690"
     CDS             4760..5188
                     /locus_tag="PKV30_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30130.1"
                     /translation="MTVMNPLYLYDDEIARLRAAVRRIRFDRFNMMLNQFHVNGKRAL
                     LHACAHPAFAALRWDIDRAALSAGIVPHAAPNPHVTLSSNANGLRIPSMRTSIQWPVD
                     EVSLVWSQRTQETGQRQYVVLERYTANASDWQPSQLDFSF"
     gene            5255..5563
                     /locus_tag="PKV30_07695"
     CDS             5255..5563
                     /locus_tag="PKV30_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRL30131.1"
                     /translation="MTAAAVGLFEAKTHLSEYVARAEKGEEVIIMRHNKPVAKIVPLH
                     AKGDPAREDIPPFIQRTHDMGVPKIDLRQINKLLGDLDVEEFLKLQTRLEAERLHGKS
                     "
     gene            5569..5994
                     /locus_tag="PKV30_07700"
     CDS             5569..5994
                     /locus_tag="PKV30_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL30132.1"
                     /translation="MKLPDTNLLIHAANPLSPDHPIARHALDEAVREPAGIGLAWLPL
                     VGFLRVSTMARVMPNPLSVADALADVQGWLGHPNARLLQPGARHIDILARLLLECGTA
                     GNLTNDAHIAAIAIEHNAEILTFDKDFAKFTGLRYQLLS"
     gene            6054..>6213
                     /locus_tag="PKV30_07705"
     CDS             6054..>6213
                     /locus_tag="PKV30_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRL30133.1"
                     /translation="MSVADIRKSFLDFFESKGHTVVPSSPLVPGNDPTLMFTNSGMVQ
                     FKDVFLGTD"
BASE COUNT         1146 a         2054 c         1979 g         1034 t
ORIGIN      
        1 tgcgcggcat cgataccgcg ccctggcgcg ccgaaagcgt cagctttctt cccgaagacc
       61 tgcgcatcgg cgatccgctg cacgtgtttg tcgaacgcgt cggtgcccgg ctggggccgg
      121 ccagcgtgca agcgcccgtg ctgcgcgccg accatcggcc cgagtgcatg cagggctggc
      181 agccggcgat tgagtgtgcg ttatcgtcga aaaaagatgc gggccatctg tctccctccc
      241 ccgctggggg agggttgggg tgggggccag cctcgtcacc cccgtcaacg tcgccagccc
      301 ctcaccctcc ttctgcaagc tcaggacagg ccaagcctct ccccagaggg gcgagggagc
      361 aaggccgaag ctgcgaaacg cagctgactg tgaccggcct tgcgccgcgc gatctgcctt
      421 cctcactctc ccaccgcgcg ggagcgggca ggggtgaggg cagtcttcct ccttcctcca
      481 gaggggaaag atcggaagga gagtcgcctc cgtcgccccc gccaggcgct cgggtttctc
      541 gccccacaaa ggcgccgcag cacaatcacc tgcccgacgc cctgacccct acctggctgc
      601 tgcgcgagcc gctgccgctc gacgtgcagg cggacgggca gccttgccac catggcccgc
      661 tgcgcctgct gaccggtccg cgtcgcatcg aggtcggctg gtggcccgat gctgccgaag
      721 ctgcatcgaa gcctgccgcg cgcgattact tcatcgccca gaccgccgcc gccgagctgc
      781 tgtggatcta ccgcgaacgc gccactgctg ctcaacccag cgatgccgcg ccgcgctggt
      841 tcctgcaagg cctgtatgcc tgagctgtcg cgcaaggaag aggcccgccg cgccgaggcc
      901 gcgcgtcgca agaacaacgt ggttgcgctg cccaggcgat caccgcgaac ccgccagggg
      961 ggcgatcgac cggactatgc cgagttgcac tgcctcacgc acttcagctt ccagcgcggc
     1021 gcctccagcc ccgaagatct ggtgcggcgc gcctgggcac tgggttacac cgcgctggcc
     1081 atcaccgacg aatgctcggt ggccggcgtg gtgcgggcgt ggaaggagag aaacgattct
     1141 cagaaggcgc tgcaggctgg cgaggcggac gacgatcttc agcgtctgca cgcctcgcgc
     1201 acggcactgg gcgcgcacga gccgctcaag ctgctggtcg gcagtgaatt ccgtttcgaa
     1261 ggacaaggca cgctcgttgt cctcgcgcgc gatctgtacg gctggggcaa cctgtgcgaa
     1321 ttcatcaccc acgcgcgccg ggccgcgccc aagggcggct accgcctgcc gccgctggcc
     1381 gatgcgctgg tggcgctgca gggttgcgag ctgctgtggt cccccatgcg aggcacgctg
     1441 gatactcctg aatcgatagc ttcttgcgct gactggataa gcgctgcggg ccgatttgat
     1501 tcaaaaatca ccctgctggg cgaactgaat ggcgccgccg acgatgccct gtggctgtcg
     1561 cagttgcagc acctgaagga agccaccggc tgctcgctgg ccgccgcggg cgacgtgcac
     1621 ctgcacgtgc gcgccgccaa gcgcctgcac gacgtgatga ccgccatccg catcggcaag
     1681 ccggtggccg aatgcggctt cgagctgcac gccaacggcg aacgccacct gcgctcgcgc
     1741 gagcggctgg ccgaggtggt tccgcccgac ttgctggccg ccacgctggc cgtggcgcag
     1801 cgctgcactt tcacgctgga agaggtcaag tacaactacc ccagtgaagc gctgatcgag
     1861 ggtctgacgg cgaccgagac cttgcgccgg gtcacctacg caggcgcggc cgagcgttat
     1921 ccgcacggtg tgccgcccga catcgagcgc ctgatcgaga aggaactggc gctcattgcg
     1981 gaatgccgct acgaaatgtt ctttctcacc gtgcaccaca tcgtgtgctt cgccaagagc
     2041 cagggcatcc tgtgccaggg gcgtggttcg gccgccaact cggtggtgtg ctggtgtctc
     2101 ggcatcaccg ctgccgaccc tgagaaatcc agcccgctgc tggagcgttt catcagcaag
     2161 gcgcgccaga atgaaccgcc cgacatcgac gtcgatttcg agcacgagcg acgcgaagag
     2221 gtcatccaat atatctacaa ggaatacggc cgccaccgcg ccgcgctggc cgccaccgtc
     2281 atctgctaca gaacccgaag cgccctgcgc gacgtgggca aggcgctggg cgtggatgaa
     2341 cgcctgatcg acgccttcgc caaggatcac cactggttcg acgagggcct ggccgtgcac
     2401 cgcctgggcg agctggcaga gggcgtgggc gccagcctca cgccgcgctt ggccgcgctg
     2461 tggctggagc tgaccgagca actgcgcggc gcgccgcgcc atctgagcca gcatgtgggc
     2521 ggcttcgtgc tgacggaaac acccttgcac cgtctggtgc cgctggagcc cgcggccatg
     2581 gagggcaatg ccgagtggaa agccagcgcg catgaagagc ctcgtgcacc ggctttcacc
     2641 agcgcggcga tggcgcaggt ggcgcaaggc ctgccgccgc tgcccgcgct gcgccaggtc
     2701 atccagtggg acaaggacga tctggaagcg ctgagcctga tgaaggtcga tgtgctcgcg
     2761 ctgggcatgc tcaccgccat gcggcgctgc atcgacatgg tcgaacgtcg gcgcggcgag
     2821 ccgttcggcc gctccgatat tcagagcgaa tgcccgcgaa cctacgagat gatccggcgc
     2881 gccgacaccg tgggcacctt ccagatcgaa agccgcgcgc agatgagcat gctgccgcgc
     2941 ctgaagccca ccacgttcta cgacctggtg gtcgaagtcg ccatcgtgcg gcccgggccc
     3001 atcagcggcg gcatggtgca cccgtatctg aaggcgcgcg aacgggcgcg gcggggcttg
     3061 ccgatcgaat acgagcgctc gaaacacgac atgccgggtc agaagccgcg tttggccaag
     3121 gcgctgggac gcacgctggg tatccccatc tttcaggagc aggtgatgga actggccatg
     3181 atcgcggccg attacaccgc tgaaggtgcc gaccggctgc gccgtgacat ggcggcgtgg
     3241 aagaaaaagg gcggcctgga gccgcatcgc gacaagctca tggccggcat gctgcgcaac
     3301 ggctatcagc gggcatttgc cgaacgcctc ttcgatcaga tcaagggctt cggcgaatac
     3361 ggctttcctg aaagccacgc ctacagcttt gcgctgatcg cgtatgaaag cagctggctc
     3421 aagtgccacg agcccgaggc ctttgtggcg gccatgctca attcgcagcc catgggcttc
     3481 tacggccccg cgcagctggt gcaagacgcc aagcggcatg gcgtggtggt gttgccgccc
     3541 gatgtgacga tgagcgactg ggattgcgcg cttgaagaag aatcggggtc agaagcgaaa
     3601 aaaatggcgg aaaaaatggt ggaaaaaatg gggtcagatc aaacttattt tgaatcgaat
     3661 ccgccatttc cccttgtcaa tcaaacgcaa gcagctatca attcaggtgc attcacttcc
     3721 tctgccgcgt tgcgggagag ggttggggtg agggggctgt tcgctccctc accctctgga
     3781 cgagggttgg ggagggggca gaccacgttt gttgcaagcg ctgctgaccc ccatcccagc
     3841 cttcccccag cgggggaagg agaaacttgc cgccccgccg ttcgcctggg cctgcgtctg
     3901 gtgcaaggct tgaatgaagg cgccgcccgc cgcatcgaag ctgcccgcgc cgatcagccc
     3961 tttgccagca tcgacgattt ggcgcgccga gcccaactcg acacccgcca gctgaatcag
     4021 cttgccgcgg cggatgcctt gctcagcctg gccggccacc gacgccagca ggtgtggcag
     4081 agctcggcct tgaaggcagc gcccgaattt ctgcgcgacg cacccatcga cgaagctcct
     4141 cttgaactgg ccgccgcgcc agaaggcgag gaaatcgtgc acgactacgc ctcgatcggc
     4201 ctcacattgc ggcgccatcc ggtggcgttg ttgcgcccgc gattgcagcg ctggcgcctg
     4261 aaaagcgcgg aagaattgaa ggcgatgccg cacggccagc aagtgcgcgc ctgcggcatc
     4321 gtcattggcc gccagcagcc gggcacggcc aagggcacca tcttcgttac gctggaagac
     4381 gaaaccggct cggtcaacgt catcgtctgg aaaagtgtgc gggtcgacga agaccagcgc
     4441 aacgcgctgt tgcgctcgcg cctgctggcg gtgcagggcg agtggcaacg cgacaaggac
     4501 agcggcggcc atgtgcgcca ccttattgcg cggcgctttg tcgacctcac cccgctgctc
     4561 ggccgcattg ccggtctgac gactagccgg gactttcatt gattgccgcg aggcgtgcca
     4621 tgcctccact gcacccagcc gccaaacgca tcttcatttg ctcactggcc agccttgaag
     4681 ctcggcgggc cattgggcaa ctgcggcgtg aggtgcgatg gccggccgat ctgtggtgga
     4741 cggagccgca ccgctgcaca tgaccgtgat gaacccgctg tatttgtacg acgacgaaat
     4801 tgcaaggcta cgcgctgcgg tgcggcgcat ccgcttcgac cgattcaaca tgatgctcaa
     4861 ccagtttcat gtcaacggca agcgggcgct cctgcatgcg tgtgcgcacc ctgcgttcgc
     4921 ggcgctgcgc tgggatatcg accgtgccgc gcttagcgca ggcatcgtgc cgcatgccgc
     4981 acccaacccg cacgtcactt tgtccagcaa cgccaatggg ttgcgaattc cttcgatgcg
     5041 cacgagcatc caatggcccg ttgacgaggt aagtttggta tggtcacaac ggacgcagga
     5101 aacggggcag cgccaatacg tcgtgcttga acgatatacg gccaacgcga gcgattggca
     5161 gccatcgcaa ctggatttca gcttctgagc agggatcaag cacgtaaacg gctaaaatct
     5221 ggttgattca accaatttcc acaaaaggcc caccatgacc gccgccgccg ttggcctgtt
     5281 cgaagccaaa acacatctgt ctgaatacgt cgcccgcgct gaaaaaggcg aggaggtgat
     5341 catcatgcgg cacaacaagc cggtggcgaa gatcgtgccg ctgcacgcca agggcgatcc
     5401 ggcgcgcgag gacattccgc cgttcattca gcgcacccat gacatgggcg ttccgaaaat
     5461 cgatctgcgc cagatcaaca agctgctggg cgatctggat gtggaggaat tcctcaagtt
     5521 gcagacgcgg cttgaggcag agcggctgca cggcaagtct tgatgtcgat gaaactgccc
     5581 gacaccaacc tgctgatcca cgccgccaat ccgctcagcc ccgatcaccc gattgcccgg
     5641 cacgcactcg acgaagcggt gcgcgagccg gctggcatcg gcctggcatg gctgcccttg
     5701 gtggggtttc tgcgcgtgtc cactatggcg cgcgtcatgc ccaacccgct gtcggtcgcc
     5761 gacgcgctgg ccgatgtgca aggatggctg gggcacccga acgcgcgcct gctgcaaccg
     5821 ggcgcgcgcc acatcgatat cctcgcgcgc ctgctgctgg aatgtggcac ggccggcaac
     5881 ctgaccaacg acgcccacat cgccgccatc gccatcgagc acaacgccga aatcctcacc
     5941 ttcgacaaag atttcgcgaa gttcaccggc cttcgctatc aattactgag ctgatcgcgc
     6001 ctgatagaca agcgctagac cccgaaaaga ccatgaaaag caaccaactt cccatgtccg
     6061 tcgccgacat ccgcaagtcc ttcctcgact tctttgaaag caagggccac accgtggtgc
     6121 cgagcagccc gctggtgccc ggcaacgacc cgacgctcat gttcaccaac agcggcatgg
     6181 tgcagttcaa ggacgtgttc ctcggcaccg aca
//
LOCUS       DAOMVO010000133         6182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3419_length_6182_cov_12.858169, whole genome shotgun sequence.
ACCESSION   DAOMVO010000133 DAOMVO010000000
VERSION     DAOMVO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3419_length_6182_cov_12.858169"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKV30_07710"
     CDS             complement(<1..187)
                     /locus_tag="PKV30_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30134.1"
                     /translation="MKPLLLTTCLPLAAAVLGAVALPASAQDRIYRCGNEYTNQIKGR
                     SDCKLVEGGNVTIVRGTA"
     gene            complement(349..1764)
                     /gene="glnA"
                     /locus_tag="PKV30_07715"
     CDS             complement(349..1764)
                     /gene="glnA"
                     /locus_tag="PKV30_07715"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRL30135.1"
                     /translation="MAKSVADVMKLVSENECKFVDFRFTDTRGKEQHVTVPVSHFDED
                     KFSEGHAFDGSSIAGWKGIEASDMLLMPDPVTAHIDPFYEEPTVVLTCDVLEPGDGKA
                     YERDPRSLARRAEAYLKASGIGDTAFFGPEPEFFVFDGVRWSTEPGHSFYKIDEYEAP
                     WNSGEKLEGGNRGHRPTVKGGYFPVPPVDSTHDMRAEMVLILEAMGIPVEVFHHEVAG
                     AGQMEIGTKFSTLVERADWTQRLKHVVWNVADSYGKTATFMPKPYHGDNGSGMHVHQS
                     IWKGGKNLFAGKGYAGLSDTALHYIGGIIKHARALNAITNPTTNSYKRLVPHFEAPVK
                     LAYSSRNRSASIRIPHVASDKARRIEARFPDPMMNPYLGFSALMMAGLDGIENKIHPG
                     EAATKDLYHLPPEEDKLVPTVCHSLDQALEELDKDRAFLTKGGVFTDSMLDAYIELKM
                     TEVNRVRVQVNPAEYDMYYSL"
     gene            complement(2099..3022)
                     /locus_tag="PKV30_07720"
     CDS             complement(2099..3022)
                     /locus_tag="PKV30_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRL30136.1"
                     /translation="MQTPDSSAPTFGLIVIGDEILSGKRADKHLPKFIELLAERGLSL
                     GWAEYVGDEPARITATLKRAFASGDVVFCTGGIGATPDDHTRQCAAAALGVPLALHPE
                     AERLIHERMRDTAREKGLPYEPDRPDNRHRLNMGMFPEGARIVPNPYNKIPGFALAGR
                     AGDADPAAGPLPRGDAAPSGGSAPRAAGERGGMIYFVPGFPVMAWPMIEWALDTDWSQ
                     CFRHDAWQERSVVIYGAMEATLTPVMERVEAAHPGVKVFSLPSVDHPEHGAHIDLGVK
                     GAPAAVQAAYSDLRAWLVPFGLRYGPELVRP"
     gene            complement(3009..3878)
                     /locus_tag="PKV30_07725"
     CDS             complement(3009..3878)
                     /locus_tag="PKV30_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HRL30137.1"
                     /translation="MFDAFARALAQAFLPRVIGMTLLPLLAMTALALLLGWLWWGAAV
                     AWMQAALQGWSVLDWLNGHLSVDRPGGLAAWLAPLFVVVLATPVIVLAALTTVSLWLT
                     PALTRLVAERRFPQLARQRGASMASSIGWSAGSLLVAVGALIVSLPLWLIPPLVLILP
                     PLIWGWLTYRVMSFDALADYASADERRAILKSHRWPLLAIGVLCGYMGAAPAVVWASG
                     VLFAALFVVLVPLAIWIYTFVFVFSSLWFAHYCLAALAERRLGEPPPAAGALPPADAL
                     TSITDVSWRDANS"
     gene            complement(3986..4966)
                     /locus_tag="PKV30_07730"
     CDS             complement(3986..4966)
                     /locus_tag="PKV30_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRL30138.1"
                     /translation="MLDTLTDTFAAAQQWLFEAAVQPIAFAVGAGNLLEDGFTATGWL
                     LVGLMQIAVLLLVIAPLERLWPVEPLRDRHAVGIDVLYTLIHRLGLFKLIMFFSVEGW
                     LTSGIGWLRAHGLPTLQIDQLWPGVTDVAWVSFIIYLIVFDLVGYGVHRAQHRFHWWW
                     QLHAVHHSQRQMTMWSDNRDHLLDSVITDVIFVVVAILIGVGPSQFVALVAVTQLLES
                     LQHANLRWSWGRVGSRLLVSPSFHRRHHAVALGHEPGSTVRDPYGENFGVLFPWWDML
                     FGSADWRPGLEPTGINDQLAGRDYGRGFWRQQWLGLKRLVGADRPRRAVL"
     gene            complement(5135..5944)
                     /locus_tag="PKV30_07735"
     CDS             complement(5135..5944)
                     /locus_tag="PKV30_07735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRL30139.1"
                     /translation="MRVLIACVLIAAGACPTSASAQKATESCKPVYLTFDTGHMGVAP
                     LVADVLKQRRVRATFFAAQEATKEGDGTLGKHWAPWWKARAAEGHEFASHTWDHAYWQ
                     ADLPPAQPGGEARFRIRPSSGAQSGKTLSWTAAQYCANLQLAADRLVSITQRKVLPLF
                     RAAGGKTSPKLLAAAEGCGFKHVGWAPAGFLGDELPSGQYPNDALLKKALQDIRPGDI
                     LMAHLGIWSRQDPWAPAVLAPLVDGLQARGFCFKTLREHPDFQPWIAAHGG"
     gene            complement(5955..>6182)
                     /locus_tag="PKV30_07740"
     CDS             complement(5955..>6182)
                     /locus_tag="PKV30_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRL30140.1"
                     /translation="ISKIDYVDFKAVDTFPAPSGPDCMDISADGSLIMVGSRWAGKLT
                     LIDVAKRKVVHQVKVGKSPHGVWTLDHARRQ"
BASE COUNT         1085 a         2036 c         2066 g          995 t
ORIGIN      
        1 gcgccgtacc gcgcacgatg gtcacgttgc cgccttccac cagcttgcag tccgagcggc
       61 ccttgatctg gttggtgtat tcgttgccgc agcggtagat gcggtcttgc gccgacgcgg
      121 gcagcgccac ggcgcccagc acagcggcgg cgaggggcag gcaggtggtc agcagcaaag
      181 gtttcatggg tgtcggccgg gcgccacgcg gtcacgcggc agtgcagcaa aagttcaagt
      241 atcgagcaaa tagagcgcct tgaacacgcg gtgttcccgc cccggcagca aaaagggcgg
      301 ccaaagccgc cctttttgcc cgatttcagg ccaatcgttg tgatcgcatc acagcgagta
      361 gtacatgtcg tattcggccg ggttgacctg cacgcgcacg cggttcactt cggtcatctt
      421 cagctcgatg taggcgtcga gcatcgaatc ggtgaacacg ccgcccttgg tcaggaaggc
      481 gcggtctttg tccagctctt ccagcgcctg gtcgaggctg tggcacacgg tgggcaccag
      541 cttgtcttct tcgggcggca ggtggtacag gtccttggtg gcggcttcgc cggggtggat
      601 cttgttctcg atgccgtcca ggcccgccat catcagcgcc gagaagccca ggtaggggtt
      661 catcatcggg tcggggaagc gcgcctcgat gcggcgcgcc ttgtcgctcg ccacgtgcgg
      721 gatgcggatc gaggccgagc ggttgcggct ggagtaagcc agcttgaccg gcgcctcgaa
      781 gtgcggcacc aggcgcttgt agctgttggt ggtcgggttg gtgatggcgt tcagcgcccg
      841 ggcgtgcttg atgatgccgc cgatgtagtg cagcgccgtg tcgctcaaac ccgcgtagcc
      901 tttgcccgcg aacaggttct tgccgccctt ccagatcgac tggtgcacgt gcatgccgct
      961 gccgttgtcg ccgtggtagg gcttgggcat gaaagtagcg gttttgccgt agctgtcggc
     1021 cacgttccac accacgtgct tcaggcgctg cgtccagtcg gcgcgctcga ccagcgtgct
     1081 gaacttggtg ccgatctcca tctggccggc gcccgccact tcgtggtgga acacctcgac
     1141 cgggatgccc atggcttcga ggatcagcac catctcggcg cgcatgtcgt gcgtcgaatc
     1201 gaccggcggc acgggaaagt agccgccctt gacggtgggc cggtggccgc ggttgccgcc
     1261 ttcgagcttt tcgcccgagt tccacggcgc ctcgtactcg tcgatcttgt agaagctgtg
     1321 gcccggctcg gtgctccagc gcacgccgtc gaagacgaag aactcgggct cggggccgaa
     1381 gaaggcggtg tcgccaatgc ccgacgcctt caggtaggcc tcggcgcggc gcgccagcga
     1441 gcgcggatcg cgctcatacg ccttgccgtc gcccggctcc agcacgtcgc aggtcagcac
     1501 cacggtgggt tcttcgtaga acgggtcgat gtgggcggtg acggggtcgg gcatcaacag
     1561 catgtcggag gcttcgatgc ccttccagcc ggcgatggag gaaccgtcga acgcgtgccc
     1621 ttcgctgaac ttgtcctcgt cgaagtgcga gaccggcacg gtaacgtgct gctccttgcc
     1681 gcgggtgtcg gtaaagcgga aatcgacgaa cttgcactcg ttttcgctca cgagcttcat
     1741 gacgtcggcg acggacttgg ccatcagggt tctcctagag gtggttcaaa tgaaacgggt
     1801 tgcgggggga tttgcagcat ccgtgccagc aaaggcgtga ttgtgccaac agacatggac
     1861 agccatgaac agccatacgc gaaggacgcg aaggatccgc gaaggacgcg aaagaataca
     1921 aaaacaatga aattggcttg cagcgcttgt gtggcaagcg cgagcagcta tgtttttatg
     1981 agtgagtcaa tttcagtctt tttcgcgtcc ttcgcaaaac ctttgcgtcc ttcgcgtatg
     2041 gtttttcttt tactcgcccc aaacaccccg tttcggtgca tgcaccaaaa caatgcattc
     2101 agggccgcac caactcgggg ccgtagcgca gcccgaaagg caccaaccag gcgcgcaggt
     2161 cggagtaggc ggcctgaact gcagcgggcg cgcccttcac gcccaggtcg atgtgggcgc
     2221 cgtgctccgg gtgatcgacg ctcggcaggc tgaacacctt gacgcccggg tgcgcggctt
     2281 caacgcgttc catcacgggg gtcagcgtgg cctccatggc gccgtagatg acgaccgagc
     2341 gctcttgcca ggcatcgtgg cgaaagcact gcgaccaatc cgtgtcgagc gcccattcga
     2401 tcatcggcca ggccatgacg ggaaaccccg gtacaaaata aatcatgccc ccacgctccc
     2461 ccgctgcgcg tggtgcgctg ccccccgagg gggctgcatc cccccttggg agcggcccgg
     2521 cggcgggatc tgcatctccc gcccgcccag ccagcgcaaa ccccggaatc ttgttgtaag
     2581 ggttgggcac gatgcgtgcg ccttcaggga acatgcccat gttcagcctg tgccggttgt
     2641 cgggccgatc tggctcgtag ggcaggcctt tttcgcgcgc cgtatcccgc atgcgctcat
     2701 ggatcaggcg ctcggcctcc ggatgcagcg ccagcggcac gcccaaggcg gcggcggcgc
     2761 actggcgggt gtggtcgtcc ggcgtggcgc cgatgccgcc ggtgcagaac accacgtcgc
     2821 cgctggcgaa ggcgcgtttg agcgtggcgg tgatgcgcgc cggctcgtcg cccacgtact
     2881 cggcccagcc caacgacaag ccgcgctcgg ccagcagttc gatgaacttg ggcaggtgct
     2941 tgtcggctcg cttgccgctg aggatttcgt caccaatgac gatgaggcca aaggtggggg
     3001 cagaagagtc aggagtttgc atcgcgccag gaaacgtcgg tgatggaagt gagtgcgtcc
     3061 gccggtggta gtgccccggc ggcgggcggc ggttcgccca gccgccgctc agccagcgct
     3121 gccagacagt aatgcgcaaa ccacagcgac gagaacacga agacaaaggt gtagatccag
     3181 atggccagcg gcaccagcac cacgaacagc gcggcaaaca gcacgcccga ggcccacacc
     3241 accgccggtg cggcgcccat gtagccgcac agcacaccga tggccaagag cggccagcgg
     3301 tggcttttga ggatggcgcg gcgctcgtcg gcgctggcgt agtcggccag cgcgtcgaag
     3361 ctcatcacgc gataggtcag ccagccccag atgagcggcg gcaggatcag caccagcggc
     3421 ggaatcagcc acagcggcag gctgacgatg agcgcgccga ccgccaccaa cagcgagccg
     3481 gccgaccagc cgatggacga cgccatcgac gcgccgcgct gccgcgccag ttgcggaaag
     3541 cgccgctcgg ccaccaagcg cgtgagcgcg ggcgtgagcc acagcgacac cgtggtcagc
     3601 gccgccagca cgatgaccgg cgtggccagc acgacgacga acagcggcgc cagccacgcc
     3661 gccaggccgc cgggtcggtc aaccgacagg tggccgttca gccagtccag caccgaccag
     3721 ccctgcagcg ccgcctgcat ccacgccacg gccgcgcccc accagagcca gccgagtagc
     3781 agcgccagcg ccgtcatggc cagcagcggc agcagcgtca tgccgatgac gcgcggcaga
     3841 aaggcctggg ccagcgcgcg ggcgaaggcg tcgaacaccg gcttcagcac agcgcgcgcc
     3901 acaccatcgt tttgggcaaa tcgttcatgg tcaaattatc ctccggcgct tgtctgacaa
     3961 gcgcaagcag ctattaaaaa catagtcaca agactgccct gcgcggccga tccgcgccca
     4021 ccaggcgctt cagccccagc cactgctggc gccagaagcc gcgcccgtag tcgcgccccg
     4081 cgagttgatc gttgatgccg gtcggctcca gccccgggcg ccagtcggcg ctgccaaaca
     4141 gcatgtccca ccacggaaac agcacaccaa aattctcgcc atacggatcg cgcacggtgc
     4201 tgccgggctc gtgccccagc gccaccgcgt ggtggcggcg gtgaaagctc gggctgacca
     4261 gcagccggct gcccacgcgg ccccacgacc agcgcaggtt ggcgtgctgc aggctttcca
     4321 gcaactgcgt caccgccacc agcgcaacga attgcgacgg cccgacgccg atcaggatgg
     4381 cgacgacgac aaagatcacg tcggtgatga ccgagtcgag caggtggtcg cggttgtcgc
     4441 tccacatcgt catctgccgc tggctgtggt gtacggcgtg caactgccac caccagtgaa
     4501 agcggtgctg cgcgcggtgc acgccgtagc cgaccaggtc gaacacgatc aggtaaatga
     4561 tgaagctgac ccaggccacg tcggtcacgc cgggccacag ctggtcgatc tgcagcgtgg
     4621 gcaggccgtg cgcgcgcagc cagccgatgc cgctggtgag ccagccttcg acgctgaaga
     4681 acatgatcag cttgaacagg cccagccggt ggatcagcgt gtaaagcacg tcgatgccca
     4741 cggcgtggcg gtcgcgcagc ggctcgacgg gccacagacg ctcaagcggc gcaatcacca
     4801 gcagcagcac ggcgatctgc atcagcccga ccagcagcca gcccgtggcg gtgaagccgt
     4861 cttcgagcag gttgccggca ccgacggcaa aggcgatggg ctgcacggcg gcttcaaaca
     4921 gccactgttg cgcggcggcg aaggtgtcgg tcagggtgtc cagcatgtct tcaagcaaaa
     4981 catgcgcacc gctcgcgggg tgtgcagccc aacgggccca gtggccgcgg agcaggccat
     5041 gcgggggcac ccatggccgg ggtctccccg gacatcgggt gcgtcccccc gctgggggga
     5101 aggcgccgaa ggcggctcag gggggagcgt catgctatcc gccgtgcgcg gcgatccagg
     5161 gctgaaaatc cgggtgctcg cgcagcgtct tgaagcagaa gccgcgcgcc tgcaggccat
     5221 cgaccagcgg cgccagcacg gccggcgccc acggatcctg acgcgaccag atgcccagat
     5281 gcgccatcag aatgtcgccc gggcgaatgt cctgcagcgc ttttttcagc agcgcgtcgt
     5341 tggggtactg gccgctcggc agctcgtcgc ccagaaagcc ggccggcgcc cagccgacgt
     5401 gcttgaagcc gcagccctcg gccgccgcca gcagcttggg cgatgtcttg ccgccggcgg
     5461 cgcgaaacag cgggagcacc ttgcgctggg tgatcgacac cagccgatcg gccgccagtt
     5521 gcaggttggc gcagtactgc gcggcggtcc agctcagcgt cttgccggat tgcgcgccgg
     5581 acgaggggcg gatgcgaaag cgcgcctcgc cgcccggttg cgcgggcggc aaatcggcct
     5641 gccagtaggc gtggtcccag gtgtggctgg caaattcgtg cccttcggcg gcgcgcgcct
     5701 tccaccacgg cgcccagtgc ttgcccagcg tgccgtcgcc ctctttggtc gcctcttgcg
     5761 cggcaaagaa ggtggcgcgc acgcggcgct gcttcaacac atcggccacc agcggcgcca
     5821 cgcccatgtg gccggtgtcg aaggtcaggt acaccggctt gcaggattca gtggcttttt
     5881 gggcgctggc gcttgtaggg caagcgccag cagctatcaa aacgcaagca atcagaacgc
     5941 gcatgtgccc tgccctattg ccgccgcgca tggtccagcg tccacacgcc gtgcggcgac
     6001 ttgccgacct tgacctggtg caccaccttg cgcttggcga cgtcgatcag cgtcagcttg
     6061 cccgcccagc gcgagccgac catgatgagc gagccatcgg ccgagatgtc catgcagtcg
     6121 ggccccgacg gcgcaggaaa ggtgtcgacc gccttgaaat ccacgtagtc gatcttgctg
     6181 at
//
LOCUS       DAOMVO010000134         6052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3504_length_6052_cov_17.275471, whole genome shotgun sequence.
ACCESSION   DAOMVO010000134 DAOMVO010000000
VERSION     DAOMVO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_6052_cov_17.275471"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKV30_07745"
     CDS             complement(<1..667)
                     /locus_tag="PKV30_07745"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRL30141.1"
                     /translation="MTSSRSHPAHRARWALLPLLCALCALPAEAQTSGSAGERKAAPT
                     AAKKQAPRKPARKPAARPAPKAAPAPAPVEPAETAAPASAPAAPPVASAPTGPREADF
                     IVAVVNTEPVTNNEVRARMARMQRQLNERGQPMPPSAALQKEVLERLIAERAQLQYAR
                     DMGLKIDDAALTQAELSIARQNQLQTVEQLYQRIQSEGIAVKDFRDDVRNQVLLARLR
                     EREI"
     gene            complement(804..3242)
                     /locus_tag="PKV30_07750"
     CDS             complement(804..3242)
                     /locus_tag="PKV30_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HRL30142.1"
                     /translation="MRILDPSAVQASLANPLFSTDASRPARAASSAGLRPVALAVLTL
                     LAAPALAQTSAPLQLQATPLLAEKLPANVNAPSVVLGDRISGRTELETVIEGNAELRQ
                     PGMVARGDKLVYDQATDVVTATGGVRVNREGDRYTATSGKIKVDAVEGFFLQPTYEFI
                     ANGAHGQAERLQLLDRDRSTVFQGTYTTCQRQDGADWRPDWILRADRLELDREDDEGR
                     AHGAVLEFKGVPILPMPTMSFPLGERRKSGWLPPTIGLDNKSGLNLSVPYYWNIAPNR
                     DATFTPTVMLRRGVDVQGEFRYLENNYNGRINANLMPNDRLRDGRDRWGYFVRHNGTH
                     DTGIAAIGNVGVGLNLNRVSDNDYWRDFTGRGLSLTTRLLANDGAVSWGRGDFSASVR
                     ALKWQTLQDVSAPIVPPYDRLPQVAARWGRINDRGFDYSVEGDFTRFRADSFWTRQPN
                     ADRTFMQAQVARPFTRPWGFFTPKVQLHASQYQFDRPLSDGRTSAGRVLPTLSLDGGL
                     VFERSASYFGRAFTQTLEPRLKYVYTPYRDQSLLPNYDTGAYDFNFATIWAENAFAGH
                     DRIVDNNLVTAGLTTRLLDPDTGVEAVRLGIAQRYRFSGQQVVLPGGTPTAKGWSDIM
                     LGASLNWHESWGFDSVIQYNPDTGRNTRTTLQARYSPGPYRTVSMAYRHQRDLNSKYI
                     DFGWQWPLSGLLGGRDELDNKAPARRAGGNGSCSGRWYGVGRLNYSIQEKRLVDGILG
                     LEYDAGCWIGRVVVEKLNSSISSSTKRIMFQIEFVGLSRLGTNPLRTLRNSIPRYQVL
                     GEQTTPPSRFSAYE"
     gene            3291..4406
                     /locus_tag="PKV30_07755"
     CDS             3291..4406
                     /locus_tag="PKV30_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRL30143.1"
                     /translation="MSDAPPAPPTPSVSTAPAAVAPTWDDPARAEAFAAWLARVAPRE
                     GLRPGSLRIASADASFRRYLRLDAEDGATRIVMDAPPTHENCAPFVHVAGLMQAAGLN
                     VPRVLDWDEPQGFMLLTDLGSRTMMEEIDRDRPENNRPRYSQAVDALLKWQQASRPGD
                     LPPYDEALLRRELALFPDWYLAKHRGVAVEGALRETLDKTFDLIVARNLAAPQVYVHR
                     DFMPRNLMASSDDTLGVLDFQDAVYGPASYDIASLMRDAFLTWDEEFVLDITVRYWER
                     ARKLGLMDFEDWHSDFGSFWRAVEWMGLQRHLKVAGIFARLTLRDGKPKYLADAPRFI
                     AYIRHTAGRYRELTPLLKAIDQIEGTEAASAWAFGRV"
     gene            4773..4862
                     /locus_tag="PKV30_07760"
     CDS             4773..4862
                     /locus_tag="PKV30_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HRL30144.1"
                     /translation="MPSLQPWSLALLMTGVSLLATRTRRRSAD"
     gene            4916..>6052
                     /locus_tag="PKV30_07765"
     CDS             4916..>6052
                     /locus_tag="PKV30_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRL30145.1"
                     /translation="MSLFQWQERPASSLAGGGVIAPDERLPWPQTAAMGVQHVIAMFG
                     ATVLAPILMGFDPNLAIFMSGIGTLIFYFITGGKVPSYLGSSFAFIGVVIAATAYAGK
                     GPNANIGVALGAIIACGLVYTLIGAVVQAIGTGWIERLMPPVVTGAVVAVIGLNLAGI
                     PIKNMAASNFDSWMQLVTFVCVALVAVFTRGMVQRLLILVGLLLATLIYLLLANGMGL
                     GKPMDFSGIVNAPWLGWPKFTAPVFDSGAMLLIAPVALILVAENLGHIKAVQAMTGRN
                     LDPYIGRAFIGDGIATMVSGAAGGTGVTTYAENIGVMAATRIYSTAVFVVAALMALLL
                     GFSPKFGAFIQAIPLPVMGGVSIVVFGLITIAGAKIWVDNKVDFT"
BASE COUNT          884 a         2049 c         2088 g         1031 t
ORIGIN      
        1 cgatctcgcg ctcgcgcagg cgcgccagca gcacctggtt gcgcacgtcg tcgcgaaaat
       61 ccttgacggc aatgccctcg gactggatgc gctggtagag ctgctcgacg gtctgcaact
      121 ggttctggcg tgcgatggac agctcggcct gcgtcagcgc ggcgtcgtcg atcttcaggc
      181 ccatgtcccg cgcatattgc agctgggcgc gctcggcgat caggcgttcc agcacttcct
      241 tttgcagcgc ggcgctgggc ggcatcggct ggccgcgctc gttgagctgg cgctgcatgc
      301 gcgccatgcg cgcgcgcacc tcgttgttgg tgaccggctc ggtgttgacc accgcgacga
      361 tgaaatcggc ctcgcgcggg ccggtgggcg cgctggcgac cggcggtgcg gccggcgcgg
      421 atgcaggtgc ggcggtctcg gccggttcca ccggcgcagg ggccggcgcg gccttgggcg
      481 ccgggcgcgc cgctggcttg cgcgccggtt tgcgcggcgc ctgcttcttc gccgccgtgg
      541 gcgccgcctt gcgctcgccg gcgctgccgc tggtttgcgc ctcggctggc aacgcgcaca
      601 gcgcgcacag caggggcagc agtgcccagc gggcgcggtg ggcgggatgg gagcgtgatg
      661 aggtcatggt tggaatggct tcgctgtgcg cagcacaaat ggccgcggag ccggccgtgc
      721 cagcggccgc cgcggagcgg actgtgcccg gccgctggcg gcgttgcccg tccggggcaa
      781 gggcgtgcag cgactcaggc gggttactca tacgctgaga agcggctcgg cggcgtggtt
      841 tgctcgccca gcacctgata acgcggaatg ctgttgcgca aggtgcgcag cgggttggtg
      901 cccaggcgcg acaggccgac gaattcgatc tggaacatga tgcgcttggt cgacgagctg
      961 atggagctgt tgagcttttc caccaccacg cggccgatcc agcagccggc atcgtattcg
     1021 agacccagga tgccgtcgac cagccgcttc tcctggatgc tgtagttcag gcggccgacg
     1081 ccgtaccagc ggccgctgca gctgccgttg ccgccggcgc gccgggctgg ggccttgttg
     1141 tccagctcgt cacgcccgcc gagcagaccg ctcagcggcc attgccagcc gaagtcgatg
     1201 tacttgctgt tcaggtcgcg ctggtggcga tacgccatgc tgacggtgcg atacggcccc
     1261 ggcgaatagc gcgcctgcag cgtcgtgcgg gtgttgcggc cggtgtccgg gttgtactgg
     1321 atcacgctgt cgaagcccca gctctcgtgc cagttgagcg aagcgcccag catgatgtcg
     1381 gaccagccct tggccgtcgg cgtgccgccc ggcagcacca cctgctggcc cgaaaaacga
     1441 tagcgctgtg cgatgcccag ccgcaccgcc tcgacgccgg tatcggggtc gagcaggcgc
     1501 gtggtcaggc cggcggtgac caggttgttg tcgacgatgc ggtcgtggcc ggcaaaggcg
     1561 ttttcggccc agatggtggc aaagttgaag tcgtacgcgc cggtgtcgta gttgggcagc
     1621 aggctctggt cgcggtacgg cgtgtagacg tacttcaggc gcggctccag cgtctgcgtg
     1681 aaggcgcggc caaagtagct ggcgctgcgc tcgaacacca gcccgccgtc gaggctgagc
     1741 gtgggcagca cgcggccggc cgaggtgcgg ccgtcgctca aggggcggtc aaactggtat
     1801 tggctggcgt gcagttgcac cttgggcgtg aaaaagcccc acggccgcgt gaacggccgc
     1861 gccacctgcg cctgcatgaa agtgcggtcg gcattgggct ggcgggtcca gaaactgtcg
     1921 gcccggaaac gcgtgaaatc gccttcgacc gaatagtcaa acccgcggtc attgatgcgg
     1981 ccccagcgcg cggccacctg cggcagccgg tcgtagggcg gcacgatggg cgcgctgacg
     2041 tcttgcagcg tctgccactt gagcgcgcgc acgctggccg aaaagtcgcc gcggccccag
     2101 ctcacggcgc cgtcgttggc cagcaggcgc gtggtcagcg acaggccgcg ccccgtgaag
     2161 tcgcgccagt agtcgttgtc gctcacgcgg ttgaggttga ggcccacgcc gacgttgccg
     2221 atggccgcga tgccggtgtc gtgcgtgccg ttgtggcgca cgaagtagcc ccagcggtca
     2281 cggccgtcgc gcaggcggtc gttcggcatc aggttggcgt tgatgcggcc gttgtagttg
     2341 ttttcgaggt agcggaactc gccctgcacg tcgactccgc gccgcagcat caccgtgggc
     2401 gtgaaggtgg cgtcgcggtt gggcgcgatg ttccagtaat agggcaccga caggttcagg
     2461 ccgctcttgt tgtcgagccc gatggttggc ggcagccagc cggacttgcg ccgctcgcca
     2521 agcggaaagc tcatggtcgg catcggcagg atcggcacgc ccttgaactc gagcaccgcg
     2581 ccgtgcgcgc ggccctcgtc gtcctcgcgg tccagctcca gccggtcggc gcgcaggatc
     2641 cagtcgggcc gccagtcggc gccgtcctgg cgctggcagg tcgtgtaggt gccttgaaag
     2701 acggtggaac ggtcgcggtc cagcaactgc agccgctcgg cctggccgtg cgcgccgttg
     2761 gcgatgaact cgtaggtcgg ctgcaggaag aagccttcca ccgcatccac cttgatcttg
     2821 cccgaggtgg cggtgtagcg gtcgccctcg cggttgacgc gcacgccgcc ggtggccgtc
     2881 accacatcgg tcgcctggtc gtacaccagc ttgtcgccgc gcgccaccat gccgggctgg
     2941 cgcagctcgg cgttgccttc gatcaccgtc tccagctcgg tgcggccgct gatgcgatcg
     3001 cccagcacca ccgaaggcgc gttgacgttg gccggcagct tttcggccag cagcggcgtg
     3061 gcctgcaact gcagcggcgc ggacgtctgc gccagcgcgg gcgccgccag cagcgtgagc
     3121 accgccagcg ccaccgggcg cagtccggcg gaagacgccg cccgcgcggg gcgcgacgca
     3181 tcggtcgaga aaagagggtt agccagcgaa gcctgcactg cggatgggtc aagaatgcgc
     3241 aagcgctggc ccggtgcggg aggcggctcg ctctatagaa tcgattatcc atgagcgacg
     3301 cgcccccggc ccccccgaca ccttccgttt ccactgcccc ggccgccgtc gcccccacct
     3361 gggacgaccc ggcgcgcgcc gaggcctttg ccgcctggct ggcgcgcgtg gccccgcgcg
     3421 agggcttgcg gcccggcagc ctgcgcatcg cctcggccga cgccagcttc cgccgctacc
     3481 tgcggctgga cgccgaagac ggcgccaccc gcatcgtgat ggacgcgccg cccacgcacg
     3541 agaactgcgc gcctttcgtg catgtcgccg gcctgatgca ggccgccggg ctgaatgtgc
     3601 cgcgcgtgct ggattgggac gagccgcagg gcttcatgct gctgaccgac ctgggcagcc
     3661 gcacgatgat ggaagagatc gaccgcgatc ggcctgaaaa caaccgcccg cgctacagcc
     3721 aagccgttga tgcgctattg aaatggcagc aagccagtcg cccgggcgac ctgccgccct
     3781 atgacgaagc cctgttgcgg cgcgagctgg cgctgttccc cgactggtat ctggccaagc
     3841 accgcggcgt ggccgtggaa ggcgcgctgc gagagacgct ggacaagacc ttcgacctca
     3901 tcgtggcgcg caacctggcc gcgccgcagg tctacgtgca ccgtgacttc atgccccgaa
     3961 acctgatggc gtcatcggac gacacgctcg gcgtgctcga cttccaggac gccgtctacg
     4021 gtcccgccag ctacgacatc gccagcctga tgcgcgacgc cttcctgacg tgggacgagg
     4081 aattcgtgct cgacatcacc gtgcgctact gggagcgcgc gcgcaagctt ggcctgatgg
     4141 actttgaaga ctggcacagc gatttcggca gcttctggcg cgcggtcgaa tggatgggcc
     4201 tgcagcggca tctgaaagtc gccggcatct tcgcgcgcct gacgctgcgc gacggcaagc
     4261 ccaagtacct ggccgacgcg ccacgcttca tcgcctacat ccgccacacc gccggccgct
     4321 accgcgagtt gacgccgctg ctcaaagcca tcgaccagat cgaaggcacc gaggcggcca
     4381 gcgcgtgggc gttcgggcgt gtgtgaggtc tttctaagtc aaattggcct cttgcgtttg
     4441 ccaggcaagc gcaagcagct ataaatttga tacttgccat cgcaccgcgc cccgtgcctc
     4501 gctgtacaag acgctgcatt gaccgactca gcgccaagcc ccacgatgcg gcgcatgaca
     4561 ccctctcgcc caccgctcat accgtccgca tcgtctgcct gccggagatg gctggccacg
     4621 gcgctgttgc taccgctctg cggcgccgcg acggcgcaga cgtacaaatc ccccgattgc
     4681 gcggcgctcg cgggcacgct caacatccac tggcgcgcca tcgacgtgac gcctcggcct
     4741 ttgcgccgct gcgggccgtc cccgtggccg ccgtgccgtc gctgcaaccg tggagcctgg
     4801 cgctgctgat gaccggcgtt tcgctgctgg caacacgcac gcggcgccgc tcggccgact
     4861 gaaaccgcga agcctctacc atcgcgccac ccagaattca acaaggagcg cgaggatgag
     4921 tctttttcaa tggcaagaac ggcccgccag cagcctggcc ggcggcggcg tgatcgcccc
     4981 cgacgagcgc ctgccctggc cgcaaaccgc cgccatgggc gtacagcacg tcatcgccat
     5041 gttcggcgcc acggtgctgg cgccgatcct gatggggttt gaccccaacc tggcgatctt
     5101 catgagcggc atcggcacgc tcatctttta cttcatcacc ggcggcaagg tgcccagcta
     5161 cctgggttcg tcgttcgctt tcatcggtgt ggtgatcgcc gccaccgcct acgccggcaa
     5221 ggggcccaac gccaacatcg gcgtcgcttt aggcgccatc atcgcgtgcg ggctggtcta
     5281 cacgctgatt ggcgccgtgg tgcaggccat cggcaccggc tggatcgagc ggctcatgcc
     5341 gccggtggtc accggcgccg tcgtggcggt gattggcctc aacctggccg gcatcccgat
     5401 caagaacatg gcggccagca acttcgacag ctggatgcaa ctggtcacct ttgtctgcgt
     5461 ggcgctggtg gcggtgttca cgcgcggcat ggtgcagcgg ctgctgattc tggtcggcct
     5521 gctgctggcc acgctcattt acctgttgct ggccaacggc atgggcctgg gcaagccgat
     5581 ggatttttcg ggcatcgtca atgcgccgtg gctgggctgg ccgaagttca ccgcgccggt
     5641 gttcgacagc ggcgccatgc tgctgatcgc gccggtggcg ctgatcctgg tggccgagaa
     5701 cctgggccac atcaaggccg tgcaggccat gacgggccgc aacctcgacc cgtacatcgg
     5761 ccgcgccttc atcggcgacg gcatcgccac catggtgtcg ggcgccgccg gtggcacggg
     5821 cgtgaccacg tatgccgaga acatcggcgt gatggccgcc acgcgcattt actccaccgc
     5881 cgtgttcgtg gtggcggcgc tgatggcgtt gcttctgggc ttttcaccca agttcggcgc
     5941 cttcatccag gccattccgc tgccggtgat gggcggcgtc agcatcgtgg tgttcggcct
     6001 catcaccatt gccggcgcca agatctgggt cgacaacaag gtcgatttca cc
//
LOCUS       DAOMVO010000135         6045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3510_length_6045_cov_12.299332, whole genome shotgun sequence.
ACCESSION   DAOMVO010000135 DAOMVO010000000
VERSION     DAOMVO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3510_length_6045_cov_12.299332"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKV30_07770"
     CDS             complement(<1..637)
                     /locus_tag="PKV30_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRL30146.1"
                     /translation="MSSEALPVAPPAVQADLAAATTDAPLRSSENALAVLEVDLSRQL
                     RFAANKVVITNQRLLSRFPNETGWQSWDLRQGLTLTHHDHAGVGSLELRDAAGQLAVW
                     RFTLGHNPAALRFLGQFEQAMAALAPGGAVSRLPAEAADAEEPDEVAEDKPPSTWTLF
                     RLWRFAKPYQWQLLAGFLLTLAATAAQLVPPYLTMPLMDKVLIPFQNGQQID"
     gene            866..2992
                     /gene="cphA"
                     /locus_tag="PKV30_07775"
                     /pseudo
     CDS             866..2992
                     /gene="cphA"
                     /locus_tag="PKV30_07775"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
     gene            3072..5741
                     /gene="cphA"
                     /locus_tag="PKV30_07780"
     CDS             3072..5741
                     /gene="cphA"
                     /locus_tag="PKV30_07780"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRL30147.1"
                     /translation="MEVTRIRALRGPNLWSRRTAIEAVVTCESAERDLRTTPEFEKRL
                     REMFPSVGGLHANKPSTPISMAHALELTALALQAEAGCPVTFSRTTPTEIEGTFQVAV
                     QYTEEAVGRLAFEKAQQLCRAAAEGGSFDLAATLAELRELDEDERLGPSTGAIVDAAI
                     ARGIPIKRLTRGSLVQLGWGSQQRRIQAAEVDDTSAIAESIAQDKDLTKKLLHSAGVP
                     VPLGRPVADVEDAWTAAQEIGLPVVVKPQDGNQGKGVTVGITERSHFDVAYKAAAEYG
                     GVMVERFLPGHDFRLLVVGDRLVAAARRDPPLVIGDGQHTVRELVAEVNKDPRRGEGH
                     GTSLTKIRIDAIAEARLQAQELTPDSVPAKGQRVILRNNANLSTGGTATDVTDSVHPD
                     VAARAIEAAQTVGLDICGVDVVCETVIKPLEEQNGGIVEVNAAPGLRMHISPSFGKGR
                     DVGAAVMDHMYPQGGNGRVPVIAVTGTNGKTTTVRLTAHLLKAQGLRVGMTNTDGVYV
                     NGRQIDSGDCSGPRSARNVLAHPDVDAAVLETARGGLLREGLAFDQCDVAIVTNLGGG
                     DHLGLNYITTLEDLKVLKRVIVLNVAPTGTAVLNAADPAVVAMAPHCPGKVTFFAQDG
                     HHPVIETHRAQGQRVVFIDHGDIVCVEGAFMKRLPLARVPLTRKGQIGFQVENTMASV
                     AAAWALATPWEAIQKGLSSFISDLHGVPGRFNVFDHAGATVIADYGHNPDAIAALVAG
                     VENMPARRRSVVISGAGDRRDEDIRGQTRILGASFDDVILYQDACQRGRADGEVLALL
                     REGLQGAARTTHVEEITGEFIAIDRALGQLQAGDLCLILIDQVEEALAHIQRRVDEVA
                     NAQPKPAPRAAAKTVRKPRRSKIATTVALGLRL"
     gene            complement(5905..>6045)
                     /locus_tag="PKV30_07785"
     CDS             complement(5905..>6045)
                     /locus_tag="PKV30_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRL30148.1"
                     /translation="ARAAKAWKDIWGCGQGIGAIKQVMPAAELIARLKREYAEARQRL
                     AL"
BASE COUNT         1040 a         2077 c         2024 g          904 t
ORIGIN      
        1 tgtcgatctg ctggccgttc tggaacggga tcagcacctt gtccatcagc ggcatggtca
       61 ggtagggcgg caccagctgg gcggcggtgg ccgccagcgt cagcagaaag ccggccagca
      121 gttgccactg gtagggcttg gcgaagcgcc acagtcgaaa cagcgtccag gtggagggcg
      181 gtttgtcttc ggcgacttcg tcgggttctt cggcgtcggc ggcctccgcc ggcaagcgcg
      241 acaccgcccc ccccggcgcc agagcggcca tggcctgctc gaactgcccc agaaagcgca
      301 gcgcggcggg gttgtgcccc aaggtgaagc gccagaccgc caactggccc gccgcatcgc
      361 gcaattcgag cgagccgacg ccggcgtgat cgtggtgcgt gagcgtcagg ccctgacgca
      421 aatcccagct ctgccagcct gtttcattcg gaaaccgcga cagcagccgc tgatttgtaa
      481 taaccacctt attggcggca aaacggagtt gacgtgataa gtcaacttcc agcaccgcca
      541 gcgcgttctc agatgagcgc aggggtgcgt cggtggtggc ggcggcgagg tcagcctgta
      601 cagccggtgg ggcgacggga agggcttctg aggacatgag atccgaaaaa tcgggtcggt
      661 gaacgccggg ccgacaaccc tcttgaacaa cgatccaagg aaagcgagcg cgctccacat
      721 gaggcgaatc gaccagaacg agcccggctc gtccagcggt ggcggattgt atccccgccc
      781 ccttttggcc acggcagcca cgcccgaacc cgccggggcc gcggccagcc ccagccggac
      841 cttccccgac atcgtgatcc gccgcatggc gcacctgcgc ggccccaacg tgtggaccta
      901 ccgcccggtg atcgaggcgg tggtggacat aggcgagctg gaagacttcc cctccaacac
      961 gctgcccggc ttctaccagc ggctgaccgg ctggctgccg ggcctgatcg agcaccgctg
     1021 cagcgtcggc cggcgcggcg gctttctgat gcggctgcgc gacggcacct ggcccggcca
     1081 catcctggaa cacgtggcgc tggagctgca aacccaggcc ggcatgaaaa ccggctttgg
     1141 caaggcgcgc atgacgcacg agcgcggcgt ctacaaggtg gtcatccgca cgcgcgacga
     1201 agccgtgggc cgcaccgcgc tcgaatccgc gcgcgacctg gtcatggccg ccatcaacga
     1261 caccccgtat gacattggcg ccaccatcgc caagctgacc gacatggtcg accgccggtg
     1321 cctgggcccc agcacggcct gcatcgtcga cgccgccacc gagcgcggca ttccgcacct
     1381 gcgcctgaac gacggcaacc tggtccagtt gggccagggc gcggcgcagc gccgcatctg
     1441 gaccgccgaa accgaccgca ccagtgccat tgccgaaggc atttcgcgcg acaaggattt
     1501 gaccaagcag ctgctggccg ctgctggcgt gccggtgccc gaaggccgca cggtggaatc
     1561 ggccgaagcg gcgtgggacg cggccgaaga catcggcctg ccggtggtcg tcaagccgag
     1621 cgatggcaac cacgcgcgcg gcgtgtcgct caacctgaag acgcgcgaag aagtgatgca
     1681 ggcgtttgtc gccgcagaag ccgaaggctc cgaagtcatt gtcgaaagct tcatccccgg
     1741 ccaggaacac cgattgctgg tggtgggcgg caaggtggcg gccgccacgc gcggcgagat
     1801 cattcgcgtc ggcggcgacg gcaagtcgac catcgcgcag ctgatcgaga cccaggtcaa
     1861 caccgacccg cgccgtggcg aagaagaaac cctgccgctg gaaaccctgc gcgtggacga
     1921 cccggtgctg ctgctgctgc tgcagcgcca gtcgctgacg ccgcaaagcg tgctgccgcg
     1981 cggccaatcc gcagtggtgc agcgcaccgg caacatggcc atcgatgtca ccggccaggt
     2041 gcaccccgac atcgccgaac agatggcgct ggccgcccgc gtggtgggct tggacatcgc
     2101 cggcatcgac ctggtggtgg aagacatcgc caaacccctg gccccgcaag gcggcgccat
     2161 tgtcgaggtg aacgccggcc ccggccttct gatgcatctg aaacccgccg tggggcgcgc
     2221 gcagccggtg ggcgaagcca tcgtcgagca cctgtttccg ggtcacgaat ccgggcgcgt
     2281 gcccatcgtc ggcctgatcg gcgacgggca gagcgcgttg gctccgcgcc tgatcgctgc
     2341 gctgctgcag ttgaatgggc agcagaccgc ccttgcctgc cgcgacggcc tgttcctggg
     2401 cgcgcgccag ttgcagaaaa ccgatgcccg cggcatggac gctggcgagc gcatgctgat
     2461 caaccgcacg gtcgaggcgg ccgtgctgga aacatccgcg cgccacatcc tgaccgaggg
     2521 cctgccctac gatcgctgcc aggtcggcgt ggtcatggcc atgccgggcg ccagcgggct
     2581 ggacgacctg tacatcaccg acgacgacca gttgccgaac atcgtgcgca cccaggtcga
     2641 cgtggtgctg ccgcagggcg cggcggtgct caacgccgac gatgccgagg tgctggaact
     2701 ggccaactac agcgacggcc aggtgattct gtacagcctg gacaccgaca gcccgtcggt
     2761 ggccgcgcac cggtcgcgca agggccgcgc ggtgctggcg cgcggcaacg acatcgtact
     2821 ggccacgggt ggtgacgaaa ccgtgctgct ttcgttgtct gcgccggatt tctcttcctt
     2881 ggccagcgac ctgcctgccg acgtgcggcc ccacgtgctg gcggcggtcg ccgccgcctg
     2941 ggcgctgggc gtgccgacca gcctgattcg tggcggtttg ttgcgatttg atgccgctcc
     3001 ggcggccagc gctgtccact gattgggccg ctcgcccgtt gcatctctca gattcagtct
     3061 ttaggactta tatggaagtt acccgcatcc gcgcgctgcg cggccccaac ctgtggagcc
     3121 gccgcacggc catcgaggcc gtcgtcacct gtgaaagcgc cgagcgcgac ctgcgcacca
     3181 cgcctgaatt tgaaaaacgc ctgcgcgaga tgttcccctc ggtcggcgga ctgcacgcca
     3241 acaagcccag cacgcccatt tccatggcgc acgcgctgga gctgacggcg ctggcgctgc
     3301 aggccgaagc cggctgcccg gtcaccttca gccgcaccac gccgaccgag attgaaggca
     3361 ccttccaggt cgcggttcag tacaccgaag aagccgtcgg ccgcttggcg tttgaaaaag
     3421 cgcagcaact gtgccgcgct gccgccgaag gtggcagctt tgacttggcc gccactttgg
     3481 ccgagttgcg cgagctggac gaggacgagc gcttgggccc ctcgaccggc gccatcgtcg
     3541 acgccgccat cgcgcgcggc attcctatca agcgcctgac ccgcggcagc ctggtccagc
     3601 tgggctgggg cagccagcag cgccgcatcc aggccgccga agtggacgac accagcgcca
     3661 tcgccgagtc gatcgcgcaa gacaaggatt tgaccaagaa gctgctgcac tcggccggcg
     3721 tgcccgtgcc gctgggccgc cccgtcgcgg atgtggaaga cgcctggacg gcggcgcagg
     3781 agatcggcct gccggtggtc gtcaagccgc aggacggcaa ccagggcaag ggcgtgacgg
     3841 tcggcatcac cgagcgcagc cacttcgacg tggcctacaa ggccgcggcc gagtatggcg
     3901 gtgtgatggt ggagagattc ctgcccggtc acgattttcg tttgctggtg gtgggcgacc
     3961 gcctggtcgc cgccgcgcgg cgcgatccgc cactggtcat cggcgacggc cagcacacgg
     4021 tgcgcgagct ggtggccgag gtcaacaagg acccgcgccg cggcgagggc catggcacgt
     4081 cgctgaccaa gatccgcatc gacgccattg ccgaggcacg tctgcaggcg caggaactga
     4141 cgcccgattc ggtgcccgcc aagggccagc gcgtcatcct gcgcaacaac gccaacctgt
     4201 ccaccggcgg caccgccacc gacgtgacgg acagcgtaca ccccgacgtc gccgcgcgcg
     4261 ccatcgaggc ggcgcagacc gtggggttgg acatctgcgg cgttgacgtg gtgtgcgaaa
     4321 ccgtcatcaa accgttggaa gagcagaacg gcggcatcgt cgaagtcaac gccgccccgg
     4381 gcctgcgcat gcacatcagc ccctcgttcg gcaagggccg cgacgtcggc gcagccgtga
     4441 tggaccacat gtacccgcaa ggcggcaacg ggcgcgtgcc ggtgatcgcc gtcaccggca
     4501 ccaacggcaa gaccaccacc gtgcgcctga ccgcgcacct gctgaaggcg caaggcctgc
     4561 gcgtgggcat gaccaacacc gacggcgtgt acgtcaatgg ccgccagatc gattcgggcg
     4621 attgctccgg cccacgcagc gcacgcaacg tgctggcgca ccccgatgtc gatgccgccg
     4681 tgctggaaac cgcgcgcggt ggcctactgc gcgaaggctt ggcattcgac cagtgcgatg
     4741 tcgccatcgt caccaacctg ggcggcggcg atcacctggg cctgaactac atcaccacgc
     4801 tggaagacct gaaggtgctc aagcgcgtga tcgtgctcaa cgtggcgccc accggcaccg
     4861 ccgtgttgaa cgccgccgac cccgcagtgg tggcgatggc gccgcactgc cccggcaagg
     4921 tcaccttctt cgcgcaggac ggccaccacc ccgtcatcga gacgcatcgc gcgcaaggtc
     4981 agcgcgtggt cttcatcgac catggcgaca tcgtctgtgt cgaaggcgcc ttcatgaagc
     5041 gcctgccgct ggcgcgcgtg ccgctcacgc gcaagggtca aatcggcttt caggttgaaa
     5101 acactatggc ctctgtggcc gccgcctggg cgttggccac gccgtgggaa gcgatccaga
     5161 agggcctgtc cagtttcatc agcgatttgc acggcgtgcc tggccgcttc aacgttttcg
     5221 accacgccgg cgccaccgtc atcgccgact acggccacaa cccggacgcc atcgcggcgc
     5281 tggtggccgg tgtcgagaac atgccggcgc gccgccgctc tgtggtcatc agcggcgcgg
     5341 gcgatcggcg cgacgaggac attcgcggcc agacacgcat cctgggcgcg tcgttcgacg
     5401 acgtgatcct gtatcaggac gcctgccagc gcggccgagc cgatggcgaa gtgctggcgc
     5461 tgctgcgcga gggcctgcag ggcgcggcgc gcaccacgca cgtggaagag atcacgggcg
     5521 aattcatcgc cattgaccgc gcgctgggcc aactgcaggc gggcgatctg tgcctgatcc
     5581 tgatcgatca ggtggaagaa gcactggcgc acattcagcg gcgcgtggac gaggtggcga
     5641 acgcgcagcc caagccagcg ccgcgcgccg ccgccaagac cgtgcgcaag ccgcgccgca
     5701 gcaagattgc caccaccgtg gcgctgggtt tgcggctctg acattcgcac cacgcatgaa
     5761 aaagcccgcc gattggcggg cttttttgtg gccgggtgat cggcaggaaa tcacattttc
     5821 atagcggtgc gcgctggttg cacaacggtt tcaagccaaa tcaccttgaa acccttgcgt
     5881 gccaagcgcg agcagctctg attttcagag cgccagccgc tggcgcgcct cggcgtattc
     5941 gcgcttcaaa cgcgcgatga gctcagccgc cggcatcacc tgcttgatgg cgccaatgcc
     6001 ctggccgcag ccccagatgt ccttccacgc cttggccgcg cgcgc
//
LOCUS       DAOMVO010000136         6037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3515_length_6037_cov_12.852558, whole genome shotgun sequence.
ACCESSION   DAOMVO010000136 DAOMVO010000000
VERSION     DAOMVO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3515_length_6037_cov_12.852558"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /gene="xrtH"
                     /locus_tag="PKV30_07790"
     CDS             <1..515
                     /gene="xrtH"
                     /locus_tag="PKV30_07790"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HRL30149.1"
                     /translation="RFFAIFLVIQAVLFGVEMLNPVQVAVVHPWTGWLAKASAAIVHV
                     IDPSVISYGRVLQSAKTGFGVSIEAGCNGVEATIVLVAAMLAFPSSWKMKLWGIGLGF
                     IAVQAVNLIRIVSLFFLGQWNFKVFEFAHLYMWQALIMLDVLVVWLLWMRYVAKHDPK
                     PAVTNAAAAA"
     gene            526..1167
                     /locus_tag="PKV30_07795"
     CDS             526..1167
                     /locus_tag="PKV30_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30150.1"
                     /translation="MRATLIGRFLLLAIVWLAVLLPLWYWAAKWFAAPPIWLAGTVMK
                     SLFSWVDGFAQEGVNAVLHTLVQVRMQGPTGDALGELAPEVSYPTYGYGLVLLWAMLL
                     ASRTERWWLKGLVGSLLLIPVQAWGICFQWLRDVVILSGPSGAAYLQYPRWVNDVVAY
                     GYQFGFLMLTPVAPIMLWLMFNKRFVAALWLEAALDEVPEQRVQASTVDSRNS"
     gene            complement(1220..2716)
                     /locus_tag="PKV30_07800"
     CDS             complement(1220..2716)
                     /locus_tag="PKV30_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRL30151.1"
                     /translation="MKPSLHLRRLPLVRPLVWAAALTTLGVVPAQAQSLLQLYDTAHG
                     FDAPYQSALANAQAAQARADQARAGLLPQVGLQMGAQRNWAETSVAGNTGSRSFNVLN
                     GSVVGSQPLYRPANRIAWDQGKRAAEAAQVQLSGTEQDLIVRLSQAYFEALAAEDALN
                     FVRTLKAAVAVQLEAAKRNFEVGNATITDSREAEARFDLATAQEIAAENDLRVKRLAL
                     DQLVGLVGTQPQPLAQPIVLPQAQPQDVNTWVDWAVNQHPAVVQSRMALDIARLETAK
                     ARTGHQPTVDLQASVGRSRYPDGNPSVSAAPNASYRGTNAGIGVVLNWPLFAGFAIEN
                     RVRETVSLEERARADLDNIQRTVAQGTRAAFFGVQSGHGQVAALEAAERSSLTSLEAN
                     QLGYQVGVRINIDVLNAQSQLYQTRRDLARARYDVLMGLLRLKQASGTLTPADLLPIN
                     QMLAVPGAPAPAVVTPLSAPPAAEAASAPAGTGRKPGSAPRPGRADRR"
     gene            complement(2916..3260)
                     /locus_tag="PKV30_07805"
     CDS             complement(2916..3260)
                     /locus_tag="PKV30_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL30152.1"
                     /translation="MIEQVTPSQLPAWLQAAADGGALPLLLDVREPAEWQAASVRVGG
                     AELLQLPMQSIPPRLSELDPERPVAVLCHHGGRSMQVAMFLAQHGFERVANVAGGIDA
                     WSLQVDPSVPRY"
     gene            complement(3305..3979)
                     /locus_tag="PKV30_07810"
     CDS             complement(3305..3979)
                     /locus_tag="PKV30_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRL30153.1"
                     /translation="MNVEQARFNMIEQQIRPWDVLDAHVLDLLAAVRREDFVPPQHRA
                     LAFADLQIPLKDSEAAVAAGQVMLEPRIEARLLQDLAVAKHEKVLEIGTGSGFMAALL
                     GHRAQRVLTLEIDNDLAQTAHANLQRAGVLNVEVRVADGASADLSADGPFDAILLSGS
                     VAQLPEALLAMLKPGGRLMGIVGDDPIMRATLVYQSPAGAVVTQPWDENVPRLLNFPE
                     PSRFRF"
     gene            complement(4020..>6037)
                     /locus_tag="PKV30_07815"
     CDS             complement(4020..>6037)
                     /locus_tag="PKV30_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL30154.1"
                     /translation="GFTINMITLMALSLCVGLLIDDAIVVRENIVRHAQMGKNSFAAA
                     MDGTQEIGLAVLATTLSIVAVFLPIGFMQGIIGKFFHEFGLTIVAAVLISMFVAFTLD
                     PMLSSVWHDPAVQTHGKPQGAPVTLYDKTLGRVTGWFDRATDRLAGGYERLLRWALKH
                     KLITLLIAGAIFALSVAMVRLLGTEFVPQADFSETNVAFYTPQGSSLQATEAKARQVD
                     DIIRGFPEVRYTVTTINSGVAAGKTNANLYIRLVDRHQRTRSAQQLAAAFRERLRAVP
                     GITVTQAGLLEPVGGQKQIEFSLQGPDQAELERLARPLMEQLRAIPGLVDLDSSSKPD
                     KPTVELTVKREAASELGLSTAQIATPLRTLVAGTITGNWRAPDDQTYDVIVRLAPESR
                     TTLQDLERLPLIAGLNADGTPRIVRLNQVATLTEATGTNQINRRAMLREIQITANVYG
                     RTTGEVSGDIRTLLDKIAFPPGYGFTFGGATKNMQESFAYALQALAMAIIFIYMILAS
                     QFKSFLQPMALMTSLPLTLIGVVLALMMFGSSMSMFSIIGIVMLMGLVTKNAILLVDF
                     AIRAREGRAGETGHSTPLAREPALLAAARVRLRPILMTTLAMVFGMVPLAFAVSEGSE
                     QRAPMGQAVIGGVITSSLLTLVVVPVVYCYLDDLAGWFKRRLRTSAA"
BASE COUNT          935 a         1976 c         2099 g         1027 t
ORIGIN      
        1 ttcgcttctt cgccatcttc ctcgtcatcc aggccgtgct gttcggcgtg gagatgctca
       61 acccggtgca ggtggccgtg gtgcacccgt ggaccggctg gctggccaag gccagcgcgg
      121 ccatcgtgca cgtcatcgac cccagcgtga tctcgtacgg ccgcgtgctg caaagcgcca
      181 agacgggctt tggcgtgtcg atcgaggccg gctgcaacgg cgtcgaggcg accatcgtgc
      241 tggtcgcggc catgctggcg tttccgtcga gctggaagat gaagctgtgg ggcatcggcc
      301 tgggcttcat cgcggtgcag gcggtcaacc tgattcgcat cgtcagcctg ttcttcctgg
      361 gtcaatggaa cttcaaggtg ttcgagtttg cgcacctcta catgtggcag gcgctcatca
      421 tgctcgacgt gctggtggtg tggctgctgt ggatgcgcta tgtggccaag cacgacccca
      481 agccggccgt gaccaacgcg gcggccgctg cctgaaggcc ccgccatgcg cgccaccctg
      541 atcggccgat tcctgttgct ggccatcgtc tggctggccg tgctgttgcc gctgtggtac
      601 tgggccgcca agtggtttgc cgcgccgcct atctggctgg ccggcacggt gatgaaaagc
      661 ctgttcagct gggtcgacgg cttcgcgcaa gagggcgtca acgccgtgct gcacacgctg
      721 gtgcaggtgc gcatgcaggg gccgacgggc gatgcgctgg gcgaactggc gccggaggtc
      781 agctacccga cctacggata cggcctggtg ctgctgtggg ccatgctgct ggcctcgcgc
      841 accgagcgct ggtggctcaa ggggctggtc ggcagcctgc tgctgattcc ggtgcaggcc
      901 tggggcatct gctttcagtg gctgcgcgac gtggtgatcc tgtcgggccc gtcgggcgcg
      961 gcctacctgc agtacccgcg ctgggtcaac gacgtggtgg cctatggtta ccagttcggc
     1021 tttctgatgc tgacgccggt ggcgccgatc atgctgtggc tgatgttcaa caagcgcttt
     1081 gtcgccgccc tgtggctcga agcggcgctg gacgaggtgc ccgaacagcg cgttcaggcg
     1141 tccacggtgg acagcagaaa ttcctgaatt gatagctgct cgcgcttgat ggacaagcgc
     1201 tggcggccat ttcaatgctt caacgccgat ccgcgcggcc cggtcggggc gccgaacccg
     1261 gcttgcggcc agtgcccgcg ggcgcactgg ccgcctcggc ggccggcggc gcggacagtg
     1321 gcgtcaccac cgccggcgca ggggcacccg gcaccgccag catctggttg atgggcagca
     1381 gatcggccgg cgtcagcgtg ccgctggcct gcttcaggcg cagcaggccc atcagcacgt
     1441 cgtagcgcgc gcgcgccaaa tcgcgccgcg tctggtagag ctggctttgc gcgttcagca
     1501 cgtcgatgtt gatgcgcacg ccgacctggt agccgagttg gttggcctcc agcgacgtca
     1561 ggctggagcg ctcggccgct tccagcgccg ccacctggcc atggcctgat tgcacgccga
     1621 agaaggcagc gcgcgtgccc tgcgccacgg tgcgctggat gttgtcgaga tcggcgcgcg
     1681 cccgctcttc cagcgacacc gtctcgcgca cgcggttctc tatggcaaaa ccggcaaaca
     1741 gcggccagtt cagcaccacg ccgatgccgg cgttggtgcc gcggtagctg gcgttgggcg
     1801 cggcgctgac tgaagggttg ccatcggggt agcggctgcg ccccacgctg gcctgcagat
     1861 cgacggtggg ctggtggccg gtgcgcgcct tggcggtttc aagccgggca atgtcgagcg
     1921 ccatgcgcga ctgcaccacc gccgggtgct ggttgacggc ccagtcgacc caggtattga
     1981 catcttgcgg ctgcgcctgc ggcagcacga tgggctgcgc cagcggctgc ggctgggtgc
     2041 cgaccaggcc taccaattgg tcgagcgcca ggcgcttgac gcgcaagtcg ttctcggccg
     2101 cgatctcctg cgcggtggcc aggtcgaagc gcgcctcggc ctcgcggctg tcggtgatgg
     2161 tggcgttgcc gacctcgaag ttgcgcttgg ctgcttcgag ctgcacggcc actgcggctt
     2221 tcagggtgcg cacgaagttg agcgcgtcct cggccgccag ggcttcgaaa tacgcctgcg
     2281 acagccgcac gatcaggtcc tgctcggtgc cagagagttg cacctgcgcg gcctcggcgg
     2341 cgcgcttgcc ctggtcccac gcgatgcggt tggccggccg gtacagcggc tgcgagccga
     2401 ccacagagcc gttcagcacg ttgaacgagc ggctgccggt gttgccggcg acgctggttt
     2461 cggcccagtt gcgctgcgct cccatctgca agccgacctg cggcaacagg ccggcgcgcg
     2521 cctggtcggc ccgcgcctgc gccgcctgcg cgttggccag cgccgactgg tagggcgcgt
     2581 cgaagccgtg cgccgtgtcg tacagctgaa gcagcgattg cgcctgcgcg ggcacaaccc
     2641 ccaacgtggt caaagcggcc gcccaaacga gcggacgaac aagcggcaga cgcctgaggt
     2701 ggagcgaggg tttcatttca gttgtccata gcgctgcgcg ccgtacgcgg tgcatgaaac
     2761 gtcagcggta cacgatgaca aatgaccgcg ctctggcgag cgcatgacaa atgactgaac
     2821 gcacgcaccg gcgcggcgcc ttgatgattt gtcatggtgc gccagcgccg gtcattgagc
     2881 gcagcgaagt cattgcagcg ggtccgggag ccatatcaat aacgggggac ggacggatcc
     2941 acctgcagcg accaggcgtc gatgccgcca gccacattgg ccacgcgctc gaagccgtgc
     3001 tgcgccagga acatcgccac ctgcatgctg cgcccgccgt ggtggcacaa cacggccacg
     3061 gggcgctcag ggtccagctc gctcaggcgc ggcgggatgc tttgcatggg cagttgcagc
     3121 aactcggcgc cgcccacccg cacgctggcg gcttgccatt cggctggctc gcgcacgtcc
     3181 agcagcagcg gcagcgcgcc accgtcggcg gcggcctgca gccaggccgg cagctggctc
     3241 ggggtgactt gctcgatcat ggtcaaatca ggctctggcg cttgtctggc gggcggtggt
     3301 tgcttcaaaa acggaagcgc gacggttcgg gaaagttgag caggcgcggc acgttttcgt
     3361 cccacggctg cgtcaccacg gcgccggccg gcgattggta gaccagcgtg gcgcgcatga
     3421 tcgggtcgtc gccgacgatg cccatcagcc ggccgcccgg cttcagcatg gccagcagtg
     3481 cctcgggcaa ctgggccacc gagccggaca gcaggatggc gtcgaacggg ccatcggccg
     3541 acaggtcggc acttgcgcca tcggccaccc gcacctccac gttcagcacg ccggcgcgct
     3601 gcaggttggc gtgcgcggtt tgcgccaaat cgttgtcgat ctccagcgtc agcacgcgct
     3661 gcgcgcggtg accgagcagc gcggccatga agccggagcc ggtgccgatt tccagcacct
     3721 tctcgtgctt ggccacggcc aggtcttgca gcagtcgcgc ttcgatgcgc ggctccagca
     3781 tcacctggcc ggcggcgacg gcggcttcgc tgtccttcag cggaatctgc aggtccgcaa
     3841 aggccagcgc ccggtgctgc ggcggcacga agtcttcgcg ccgaacggcg gccagcaggt
     3901 cgagcacgtg ggcgtcgagc acgtcccagg gacgaatctg ctgctcgatc atgttgaagc
     3961 gggcttgttc gacgttcatg tgaagtcctt gaagagctgg ggcgggcaaa ccttgaattt
     4021 taggcggcag acgtgcgcaa ccggcgcttg aaccagccgg ccagatcgtc caggtagcaa
     4081 tacaccacgg gcaccaccac cagcgtcagc agcgacgagg tgatgacacc gccgatgacg
     4141 gcctgcccca tcggcgcgcg ctgctcggag ccttcgctga cggcaaaggc cagcggcacc
     4201 atgccgaaca ccatcgccag cgtggtcatc aaaatcggcc gcagccgcac gcgcgcggcg
     4261 gccagcaggg cgggctcgcg cgccagcggc gtgctgtggc cggtttcgcc ggcgcggcct
     4321 tcgcgcgcgc ggatggcgaa atcgaccagc aaaatcgcgt tcttggtcac cagccccatc
     4381 agcatgacga tgccgatgat cgagaacatc gacatgctgc tgccaaacat catcagcgcc
     4441 agcaccacgc cgatcagcgt gagcggcagc gaggtcatca gcgccatcgg ctgcaagaag
     4501 ctcttgaact ggctggccag gatcatgtag atgaagatga tcgccatggc cagcgcctgc
     4561 agtgcgtagg caaacgattc ctgcatgttc ttggtggcgc cgccaaaggt gaagccgtag
     4621 ccgggcggaa aggcgatctt gtccagcagg gtgcggatgt cgcccgacac ctcgcccgtg
     4681 gtgcggccgt agacgttggc ggtgatctgg atctcgcgca gcatggcgcg gcggttgatc
     4741 tggttggtgc cggtggcctc ggtcagcgtg gcgacctggt tcaggcgcac gatgcgcggc
     4801 gtgccgtccg cgttcaggcc ggcgatgagc ggcaggcgct ccaaatcttg cagcgtggtg
     4861 cgcgattcgg gcgccagccg cacgatgacg tcatacgtct ggtcgtccgg cgcgcgccag
     4921 ttgccggtga tggtgccggc caccagcgtg cgcagcggcg tggcgatttg cgcggtggac
     4981 aggccgagct ccgacgcggc ttcgcgcttg acggtcagtt ccaccgtcgg cttgtcgggc
     5041 ttgctgcttg aatccagatc gaccaggccg gggatggcgc gcaactgctc catcagcggg
     5101 cgcgccagcc gctccaactc ggcctggtca ggcccttgca gcgagaactc gatctgcttt
     5161 tgcccgccca ccggctcgag caggccggcc tgcgtgaccg tgatgccggg caccgcgcgc
     5221 agccgttcgc ggaaggcggc cgccagttgc tgcgcgctgc gcgtgcgctg gtgccgatcc
     5281 accagccgga tgtacaggtt ggcgttggtc ttgcccgccg ccacgccgct gttgatggtg
     5341 gtgacggtgt agcgcacttc agggaagccg cggatgatgt cgtccacctg ccgcgccttg
     5401 gcctcggtgg cctgcagcga cgagccttgc ggggtgtaga aggcgacgtt ggtctcggag
     5461 aaatccgcct gaggcacgaa ctcggtgccc aagaggcgca ccatcgccac gctgagcgca
     5521 aagatcgcgc ccgcgatcag cagcgtgatg agcttgtgct taagcgccca gcgcagcagc
     5581 cgttcgtagc cgccggccag ccggtcggtc gcgcggtcga accagccggt gacgcggccc
     5641 agcgtcttgt cgtacagcgt gacgggcgcc ccctgcggct tgccatgcgt ttgcaccgcc
     5701 gggtcgtgcc acacggacga cagcatcggg tcgagcgtga aggcgacaaa catcgagatc
     5761 agcaccgccg ccacgatggt caggccgaac tcgtgaaaga acttgccgat gatgccctgc
     5821 atgaagccga tgggcagaaa caccgccacg atcgacagcg tggtggccag caccgccaga
     5881 ccgatctctt gcgtgccgtc catggcggcg gcaaaactgt tcttgcccat ctgcgcatgg
     5941 cgcacgatgt tctcgcgcac cacgatggcg tcgtcgatca agaggcccac gcacaggctc
     6001 agcgccatca gcgtgatcat gttgatggtg aagccga
//
LOCUS       DAOMVO010000137         6032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3520_length_6032_cov_13.801071, whole genome shotgun sequence.
ACCESSION   DAOMVO010000137 DAOMVO010000000
VERSION     DAOMVO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3520_length_6032_cov_13.801071"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="hemA"
                     /locus_tag="PKV30_07820"
     CDS             complement(<1..1022)
                     /gene="hemA"
                     /locus_tag="PKV30_07820"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRL30155.1"
                     /translation="MAVWALGLNHHTAPLDLRGRFAFALDQMAPALAGLRQSHARHPE
                     AAILSTCNRTEVYCASDNVQIDHTLGWLAQAGDVPAHALRDHAYVLQNDQAARHAFRV
                     ASGLDSMVLGEPQILGQMKSAVRAAEDAGALGQTLNQLFQRSFAVAKQVRSSTEIGAH
                     SISMAAAAVRLAGNLFEDLREIKVLFVGAGEMIELAATHFAAKSPKSIAIANRTLERG
                     EKLAGRLGGEVMRLADLPERLHEFDAVVSCTASTLPIIGLGAVERALKKRRHRPIFMV
                     DLAVPRDIEPEVKALQDVYLYTVDDLAQVVQTGHANRQAAVAQAEAIIDAGVQSFMHW
                     VDQRDPA"
     gene            1101..1373
                     /locus_tag="PKV30_07825"
     CDS             1101..1373
                     /locus_tag="PKV30_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30156.1"
                     /translation="MDLVDFIWHLVGFVLPALALAPAVVLIARFLGRKSPSARSLQAQ
                     LAINFAVCVAVLLAGLALTGHDGRMLTYAALVLASATTQVLLSRRS"
     gene            complement(1378..2112)
                     /gene="ugpQ"
                     /locus_tag="PKV30_07830"
     CDS             complement(1378..2112)
                     /gene="ugpQ"
                     /locus_tag="PKV30_07830"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRL30157.1"
                     /translation="MTDWPYPFWIAHRGAGRLAPENTLAAFRHGAAHGYRMAECDAKL
                     SADGVPFLLHDSTLERTTSGAGMAGDLAWGALSQLDAGSWHSRAFAGEPIPTLENLAR
                     WCLANKHLIDIEIKPTPGLEEKTGQVVAETAARLWQGAAVPPLLTSFRPESLLGALQA
                     APSLPRGLLLDTLWDGCFGYAEQLKCAAIVCNHALWDAALVAKVHAMGMRALSYTVND
                     EWAAERLIALGTDGIVTDRVDLFSPA"
     gene            complement(2196..3227)
                     /locus_tag="PKV30_07835"
     CDS             complement(2196..3227)
                     /locus_tag="PKV30_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRL30158.1"
                     /translation="MKAAFITGHGGNDVVQVGERPGPERAPGEVRVRLHAATLNRVDL
                     YMRDSGAGITHRLPQILGLDGAGVIDEIDASDALLQPGQRVLLHPGIACGRCEFCLRG
                     GDVLCAHYQLIGEHRDGTFAQFVSVPARNVLPLPDDWSFAEGAAIGVNHLTAWRMLVT
                     QAQMQPWETVLIFGIGGGVSLAGLQLAKALGARCIVTSRHAGKLEQARALGADHVIDA
                     SREDIAKTVLAATGGRGVDVVFENVGEAVWPAAMKSLVRGGRLVTCGATSGDQPGADL
                     RRLFIRQLRVIGSTLGTVSELHDLLAFCQRHGVRPVIEQTFALDQVHAALDRLESGEQ
                     FGKIALAID"
     gene            complement(3289..4311)
                     /gene="ugpC"
                     /locus_tag="PKV30_07840"
     CDS             complement(3289..4311)
                     /gene="ugpC"
                     /locus_tag="PKV30_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRL30159.1"
                     /translation="MAAIEFNNVIKRYGTGPKANQVIHGVNAEVKDGEFVVIVGPSGC
                     GKSTLLRMVAGLEEITDGTISIGGRVVNQLEPAERDIAMVFQNYALYPHMSVFDNMAY
                     GLKIKKVPTDEINARVDKAARILELGSFLQRKPRELSGGQRQRVAMGRAIVRQPQVFL
                     FDEPLSNLDAKLRAQTRLEIEKLHRELGITSLFVTHDQVEAMTLAQRMMVMNGGRMEQ
                     FGTPEEVYHRPATTFVASFIGAPPMNLLTGVEGVREGVVLGVRPEHLRVVDSGGWPLQ
                     VETVEMLGAERLIYGHLGGAQGPWTILRMHETEAPPASGQTLHVVPLEDRVHGFDAAS
                     GRRIES"
     gene            complement(4315..5172)
                     /gene="ugpE"
                     /locus_tag="PKV30_07845"
     CDS             complement(4315..5172)
                     /gene="ugpE"
                     /locus_tag="PKV30_07845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HRL30160.1"
                     /translation="MIENRPGTTAIAHTVLLLGVLAVGFPVYLAFVASTQTSAEIVQN
                     VPMSLLPGSHLVDTYRLALFGGETSYGSRIPPAGRMLWVSFVTAIVIAVGKIAISMLS
                     AFAIVYFRFPFRGLVFWMIFITLMLPVEVRILPTYKVVADLNMLNSYTGLTLPLIASA
                     TATFLFRQFFLTVPDELTEAARIDGAGPLRFFKDVLLPLSKTSMAALFVIQFIYGWNQ
                     YLWPLLMTTTEDMYPIVMGIKRMFAGGDAANEWNVIMATAILAMIPPALVVVLMQKWF
                     VKGLVDTEK"
     gene            complement(5210..6028)
                     /gene="ugpA"
                     /locus_tag="PKV30_07850"
     CDS             complement(5210..6028)
                     /gene="ugpA"
                     /locus_tag="PKV30_07850"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HRL30161.1"
                     /translation="MVLVFFFWPAGQAIYQSFLREDAFGTSTEFVGIDNFVELFNDAS
                     YLASFKTTLIFSVLVAVLGLVISLMLAVMADRVIKGATGYKTLLIWPYAVAPAVAGVL
                     WLFMFAPSIGVVAWALRRVGFDWNHLLNGTHAMALIVMAAVWKQISYNFLFFLAGLQS
                     VPKSLIEAAAIDGAGPWRRFWTVQFPLLSPTTFFLLVMNVIYAFFDTFGIVDAATHGG
                     PGKDTAILVYKVYFDGFKGLDLGGSAAQSVVLMVIVIALTVFQFRYVEKKVNYS"
BASE COUNT         1032 a         1952 c         2029 g         1019 t
ORIGIN      
        1 gccgggtcgc gctggtcgac ccagtgcatg aagctttgca cgccggcgtc gatgatggct
       61 tcggcctgcg cgacggcggc ctggcggttg gcgtggccgg tttgcacgac ctgggccagg
      121 tcgtcgacgg tgtacaggta cacgtcctgc agcgccttga cctcgggctc gatgtcgcgc
      181 ggcacggcca ggtcgaccat gaagatcggg cggtggcggc gctttttcag cgcgcgttcg
      241 acggcgccca ggccgatgat gggcaaggtg ctggcggtgc agctgacgac ggcgtcgaac
      301 tcgtgcaggc gctcgggcag gtcggctagg cgcatcactt caccacccaa gcggccggcc
      361 agtttttcgc cacgctccag cgtgcggttg gcgatggcga tggattttgg gctcttggcg
      421 gcaaagtgag tggccgccaa ctcgatcatc tcgccggcgc cgacaaacag caccttgatc
      481 tcgcgcaggt cttcaaacag gttgccggcc aggcgcacgg cggcggcggc catgctgatg
      541 gaatgggcgc cgatttcggt cgatgaacgc acctgcttgg cgacggcaaa gctgcgctga
      601 aagagctggt tcagcgtttg gcccagcgcg cccgcatcct cagcggcgcg cacggcgctt
      661 ttcatctggc ccaggatctg cggctcgccc agcaccatgc tgtcgagtcc gctggcaacg
      721 cgaaaggcgt ggcgggcggc ctggtcgttt tgcagcacgt aggcgtggtc gcgaagggcg
      781 tgcgcgggca cgtcacccgc ctgcgccaac cagcccaggg tgtggtcgat ctgcacgttg
      841 tccgacgcgc aatacacctc ggtgcggttg caggtggaga ggatggcggc ttcaggatga
      901 cgggcatgcg actggcgcag cccggccagc gcgggcgcca tctggtcgag cgcgaacgca
      961 aaccgcccgc gcagatcgag cggtgcggtg tggtgattga gtcccaatgc ccagacggcc
     1021 atggcggcga ttataaaatt gagggcgcgt tcgtggttcc cgatgggctt cggacttgac
     1081 ccgcatcaag aacccttccc atggatctcg tggatttcat ctggcacctg gtcggttttg
     1141 tgctgccagc gctggccttg gcgccggcgg tggtgctgat cgcgcggttc ttgggtcgaa
     1201 aaagcccgtc cgcgcgctct ctgcaagcgc agctagctat caatttcgca gtctgcgtgg
     1261 cggtgctgct ggccggcctg gcgctgaccg ggcacgacgg ccgcatgctg acctacgccg
     1321 cgctggtgct ggcaagcgcg acgacgcagg tgctgctgtc gcggcgcagt tgaccgctta
     1381 agccgggctg aacaaatcca cccggtcggt cacgatgccg tcggtgccca gggcgatcag
     1441 gcgctctgca gcccattcgt cgttcacggt gtagctcagc gcgcgcatgc ccatggcgtg
     1501 cactttggcg accagcgccg cgtcccacag cgcgtggttg cagacgatgg cggcgcactt
     1561 cagttgttcg gcgtagccga agcagccgtc ccacagcgtg tcgagcagca gcccgcgcgg
     1621 cagggacggg gcggcttgca gcgcgcccag cagcgattcg gggcggaagg aggtgagcag
     1681 cggtggcacg gctgcgcctt gccacaggcg ggcggcggtt tctgcaacga cttggccggt
     1741 tttttcttcc aggccaggcg tcggcttgat ctcgatgtcg atcaggtgct tgttggccag
     1801 gcaccagcgc gccaggttct caagcgtcgg aatcggttcg ccggcaaacg cgcgcgaatg
     1861 ccagctgccg gcatccagct gcgacagcgc gccccacgcc agatcacccg ccatgccggc
     1921 gccgctggtg gtgcgctcca gcgtgctgtc gtgcagcaga aagggcacgc cgtcggcgga
     1981 cagcttggcg tcgcattcgg ccatgcggta gccgtgcgcg gcgccgtgtc gaaacgcggc
     2041 cagcgtgttt tccggcgcca gccggccagc gccgcggtgg gcgatccaga aggggtaggg
     2101 ccaatccgtc atcgcgcaaa gatatcaaaa atcatagctg ctcgcgctgg acagacaagc
     2161 gctggaggcc catttggctt gaattctcgc tgccatcagt cgatcgccag cgcgatcttg
     2221 ccgaactgct cgcctgattc gagtcggtcg agcgcggcgt gcacctgatc cagtgcgaag
     2281 gtctgctcga tgaccgggcg cacgccgtgg cgttggcaga aagcgaggag gtcgtgcagc
     2341 tcactcaccg tgccgagcgt ggagccgatc acgcgcagct ggcggatgaa caggcggcgc
     2401 aaatccgcgc ccggttggtc gccgctggtg gcgccgcagg tgaccaggcg gccgccgcgc
     2461 accagcgact tcatggcggc gggccagacg gcttcgccca cgttctcgaa cacgacatcg
     2521 acaccgcgac cgcccgtggc ggccagcacg gttttggcga tgtcttctcg gctggcgtcg
     2581 atcacatgat cggcgccgag cgcgcgggct tgttcgagct tgccggcgtg gcgcgaggtg
     2641 acgatgcagc gagcgcccag cgccttggcc aattgcagcc cggccagcga cacaccgccg
     2701 ccgatgccga agatcagcac cgtctcccaa ggctgcatct gcgcttgcgt caccagcatg
     2761 cgccaggcgg tcaggtggtt cacgccgatg gccgcgcctt cggcaaagct ccagtcatcc
     2821 ggcagcggca gcacattgcg cgccggcacg ctgacgaact gcgcaaaggt gccgtcgcgg
     2881 tgctcgccga tcagctggta gtgcgcgcac agcacgtcgc cgccgcgcag gcagaactca
     2941 cagcgcccgc aggcgatgcc ggggtgcagc agcacgcgct ggccgggctg caacagcgca
     3001 tcgctggcgt cgatctcgtc aatcacgccc gcgccatcca gccccaggat ttgcggcaag
     3061 cggtgcgtga tgccggcgcc gctgtcgcgc atgtacaggt cgacccggtt gagcgtggcc
     3121 gcgtgcagcc gcacccgcac ttcgcccggt gcgcgctcag ggccggggcg ctcgcccacc
     3181 tgcaccacgt cgttgccgcc atggccggtg atgaaggctg ctttcatgtc attttggccg
     3241 ccagcgcttg ccagacaagc gcaagcagct atcaaaaata tagttttgtc aagactcgat
     3301 ccggcgcccg ctggcggcgt cgaagccgtg cacgcggtct tccagcggca ccacgtgcag
     3361 cgtctgtccc gacgcgggtg gcgcttcggt ctcgtgcatg cgcagaatcg tccacgggcc
     3421 ttgcgcgccg cccaggtggc cgtagatcag ccgctcggcg cccagcattt ccaccgtttc
     3481 gacctgcagc ggccagccgc ccgaatcgac gacgcgcagg tgctcgggcc gcacgcccag
     3541 caccacgccc tcgcgcacgc cttcgacacc cgtgagcagg ttcatcggcg gcgcgccgat
     3601 gaagctggcg acaaaggtgg tcgccgggcg gtggtacacc tcttccggcg tgccgaactg
     3661 ctccatgcgg ccgccgttca tcaccatcat gcgctgcgcc agcgtcatgg cctcgacctg
     3721 gtcatgcgtg acgaacaggc tggtgatgcc cagttcgcgg tgcagttttt cgatctccag
     3781 ccgcgtctgc gcgcgcagct tggcgtcgag gttcgagagt ggttcgtcga acaggaagac
     3841 ttgcggctgc cgcacgatgg cgcggcccat ggccacgcgc tggcgctgcc cgcccgacag
     3901 ctcgcgcggc ttgcgctgca gaaagctgcc caactccaga atcctggccg ccttgtccac
     3961 gcgcgcgttg atctcgtcgg tgggcacctt cttgatcttc aggccgtagg ccatgttgtc
     4021 gaacacgctc atgtgcgggt agagcgcgta gttctgaaac accatggcga tgtcgcgctc
     4081 ggccggctcc agctggttga ccacgcgccc accgatgctg atggtgccgt cggtgatttc
     4141 ctccagcccc gccaccatgc gcagcagcgt cgatttgccg caacccgaag ggccgacgat
     4201 gacgacgaat tcgccgtcct tgacttcggc gttgacgcca tggatgacct gattggcctt
     4261 ggggccggtg ccgtagcgct tgatgacgtt gttgaattcg atcgccgcca ttcgttattt
     4321 ctcggtgtcc acaaggccct tgacgaacca tttctgcatc agcaccacca ccagcgcggg
     4381 cgggatcatc gccaggatgg cggtggccat gatgacgttc cattcgttgg ccgcatcgcc
     4441 gccggcaaac atgcgcttga tgcccatcac gatggggtac atgtcctcgg tggtggtcat
     4501 cagcagcggc cacaggtact ggttccagcc gtagatgaac tggatgacga acagcgccgc
     4561 catgctggtc ttcgacagcg gcagcagcac gtccttgaaa aagcgcagcg ggccggcgcc
     4621 gtcgatgcgc gccgcctcgg tcagctcgtc gggcaccgtg aggaagaact ggcggaacag
     4681 gaaggtggcg gtggccgagg cgatcagcgg cagcgtcagg cccgtgtagc tgttcagcat
     4741 gttcaggtcg gccaccacct tgtacgtggg caggatgcgc acttccaccg gcagcatcag
     4801 cgtgatgaag atcatccaga acaccaggcc gcggaacgga aagcggaagt acacgatggc
     4861 gaacgccgac agcatcgaaa tggcgatctt gcccacggcg ataacgatgg cggtgacaaa
     4921 gctcacccac agcatgcggc ccgcgggcgg aatgcggctg ccatagctgg tttcgccgcc
     4981 gaacagcgcc aggcggtagg tgtcgaccag gtgcgagccg ggcagcaacg acattggcac
     5041 gttctgcacg atctcggccg aggtttgtgt ggaggcgaca aacgccaggt agaccggaaa
     5101 gccgaccgcc agcacgccca gcagcagcac ggtgtgggcg atggcggtgg tgccggggcg
     5161 gttctcgatc atggctggcg tccgcggggg gcttgcaggg caggcaggct catgagtagt
     5221 tgaccttttt ctcgacgtag cggaactgga acaccgtgag cgcgatgacg atcaccatca
     5281 gcaccaccga ttgcgcggcc gagccgccca ggtccagccc tttgaagccg tcgaaataca
     5341 ccttgtagac cagtatggcc gtgtccttgc ccggcccgcc gtgcgtggcc gcatcgacga
     5401 tgccgaaggt gtcgaaaaac gcatagatca cgttcatcac cagcaggaag aacgtggtgg
     5461 gcgaaagcag cggaaactgc accgtccaga agcgccgcca ggggccggca ccgtcgatgg
     5521 cggcggcttc aatcaggctc ttgggcaccg actgcaggcc ggccaggaag aacaggaagt
     5581 tgtatgaaat ctgcttccac accgccgcca tcacgatcag cgccatggcg tgcgtgccgt
     5641 tcagcaggtg gttccagtcg aagcccacgc gccgcagcgc ccaggccacg acgccgatgg
     5701 agggtgcgaa catgaacagc cacagcacgc ccgccaccgc cggcgccacc gcgtagggcc
     5761 agatcagcag cgtcttgtag ccggtggcgc ccttgatgac ccggtcggcc atcacggcca
     5821 gcatgagcga aatgaccagg cccagcaccg ccaccagcac cgaaaagatc agcgtggtct
     5881 tgaaggaggc gaggtagctg gcgtcgttga acagttccac gaagttgtcg atgccgacaa
     5941 attcggtgct ggtgccgaag gcgtcttcgc gcaaaaagct ctggtaaatc gcctgccccg
     6001 ccggccagaa gaaaaagacc agcacgatga cc
//
LOCUS       DAOMVO010000138         6016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3540_length_6016_cov_15.004529, whole genome shotgun sequence.
ACCESSION   DAOMVO010000138 DAOMVO010000000
VERSION     DAOMVO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_6016_cov_15.004529"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKV30_07855"
     CDS             <1..542
                     /locus_tag="PKV30_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30162.1"
                     /translation="LVYRVGQGGTPATRDLAVALSHVVRHAAQLAVSSRNWSLWGSSA
                     GARMVAAIGTHGTAAYGGDELPRPAAVVMAYTGHSEIARQGETDPPKLDDLQDFITCY
                     NPENRHERVATERFKFFSYEELMARDKASLDIFWLKDDSLDNLDDLPPPDVLQQEIIE
                     HLEAALASFRDVAVGLRSS"
     gene            complement(726..1427)
                     /locus_tag="PKV30_07860"
     CDS             complement(726..1427)
                     /locus_tag="PKV30_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL30163.1"
                     /translation="MSAPQTVLITGAAGHLGQAVAATFRQRGARLVLADRRLDALKAA
                     FDHADDVLLLPIDLMDRDGVQAGVQQALDRFGAIDACCHIAGGFRMGESVHETSAATW
                     DFLMDLNARTLINVASAVVPAMIQRGRGRIVTIGAGAALKGGAHMGAYAASKSALIRL
                     TESMSAELKHQGINVNCVMPSIIDTPDNRAAMPDADASRWVAPAALADVIAFLASDGA
                     AAIHGAAIPVTGLVD"
     gene            complement(1424..2323)
                     /gene="phhA"
                     /locus_tag="PKV30_07865"
     CDS             complement(1424..2323)
                     /gene="phhA"
                     /locus_tag="PKV30_07865"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRL30164.1"
                     /translation="MLQQLKEHVKHGLAAGTDQRPERPDWTIDQGWSQYTAQEHAVWK
                     TLFERQSKLLPGRACDEFVRGMQDLPIGPDQIPNFEQLSETLSQRTGWQIVAVPGLVP
                     DDVFFEHLAHRRFPAGHFIRKPHELDYLEEPDVFHDVFGHVPMLMNPAIADYIQAYGE
                     GGLRAKRLGVLEKLARVYWYTVEFGLVKQPDGLRVYGAGIASSATETVFSVEDDSPNR
                     VAFDLERVMRTNYRIDDFQETYFVLDNLNDLLELARIDFAPLYERVADASDFEPGDIL
                     PTDTVLTRGSGRYHAAKRDGAGA"
     gene            complement(2341..2649)
                     /locus_tag="PKV30_07870"
     CDS             complement(2341..2649)
                     /locus_tag="PKV30_07870"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRL30165.1"
                     /translation="MKLNATQLQPLLADLPQWTHHDERGGILKREFVFHDFAQAFGFM
                     GQIAVHAEKRNHHPEWFNVYNRVAVTLTTHDVGGLSMNDIDMARLMDRVAAALAQQAV
                     "
     gene            complement(2793..3665)
                     /locus_tag="PKV30_07875"
     CDS             complement(2793..3665)
                     /locus_tag="PKV30_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRL30166.1"
                     /translation="MDTTQDIKQSTQSPLANAWAELQAHADRGEPLEPDAYRLAFADP
                     EFLLRREVRGIRFQLELLKPELGLQEAGIHNTIVVYGGARFVAPDVAEQLLASARAGG
                     DAQAMALAERAMRTSRYYTQAREFGRIVATYSLRQPQEEKMFICTGGGPGVMEAANRG
                     AHDVGVPSVGLNIALPHEQETNPYVTPQLAFKFHYFALRKMHFLMRARALVAFPGGFG
                     TLDELFEVLTLVQTRKAQKVPIVLFGSDYWRRLLNLQMLVDEGAIALEDLDLYQMADS
                     PEDAWDIIRRFYGL"
     gene            complement(3781..4335)
                     /gene="efp"
                     /locus_tag="PKV30_07880"
     CDS             complement(3781..4335)
                     /gene="efp"
                     /locus_tag="PKV30_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRL30167.1"
                     /translation="MKIAQEIRAGNVIMHGKDPMIVLKTEYARGGRGAATVRMKLKAL
                     LNNMGTEVVFKADDKMEQVILDHKECTYSYFADPMYVFMDGEYNQYEVEAENMGDALN
                     YLEDGMAVEVVFYDGKAISVELPTSVEREITWTEPAVKGDTSGKVLKPAKIATGYEVA
                     VPLFVAQGDKIEIDTRTGEYRKRV"
     gene            complement(4468..5607)
                     /gene="earP"
                     /locus_tag="PKV30_07885"
     CDS             complement(4468..5607)
                     /gene="earP"
                     /locus_tag="PKV30_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HRL30168.1"
                     /translation="MPKSVPLLWDIFCRVIDNHGDLGVCWRLSCDLAARGQQVRLWVD
                     DASALHWMASGALQGEVPDVRVLPWTQPIDPALLASLPASDVWIEAFGCDIAPDFIAS
                     CADSSSTGGRFDSRTAPTWINLEYLSAEPWVERMHALPSPVQHGPAAGRTKWFFYPGF
                     TPHTGGLLREQDLAARQQAFDGAAWLQAQGIDARGERIASLFCYEPPPLGALLRQLDT
                     ATTPTRLLVTAGRAARAVARCLEDENDLQRLSGQREQLSLSYLPHLTQRAYDELLWAC
                     DLNFVRGEDSLVRALWAGAPLVWHIYPQPEDDAHHAKLGAFLDWLQAPASLRRFHHVW
                     NGIEAGPLPEIDPLGWRACVQAARQRLLEQPDLGTQLIGFVAQKR"
     gene            5650..>6016
                     /locus_tag="PKV30_07890"
     CDS             5650..>6016
                     /locus_tag="PKV30_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRL30169.1"
                     /translation="MSQPPSAPEAGAPEPTDEQTPAATGHSPELLAQVAALPGLPGVY
                     RYYDAAGNVLYVGKARHLKKRVSSYFVKNHGGTRIGHMVGKIARLDTTVVRSEAEALI
                     LENNLIKQLAPKYNILFRDD"
BASE COUNT         1027 a         1907 c         2012 g         1070 t
ORIGIN      
        1 tcctcgtcta ccgcgtgggc caaggtggca cacccgctac gcgcgacctc gcggtcgcgc
       61 tgtcgcatgt cgtgcgccat gccgcccagc tggccgtgtc atcgcgaaac tggtcgctgt
      121 ggggcagctc ggccggggcg cggatggtgg cggccatcgg gacgcatggg acggccgcgt
      181 atggcggcga cgagctgccg aggccggcag cggtcgtcat ggcctacacc ggacactcgg
      241 agatcgccag acaaggtgaa acagatcccc ccaagctgga cgatttgcag gatttcatca
      301 cctgctacaa cccggaaaat cgacacgagc gcgtagcgac cgaacggttc aagttcttca
      361 gctacgaaga attgatggct cgcgacaaag ccagtctcga catcttctgg ctcaaggatg
      421 acagcctgga caacctcgac gatctgccgc cgccggatgt gctgcagcag gagatcattg
      481 agcatctcga agcggcatta gcctcgttcc gggatgtggc ggttgggctg cggagtagct
      541 gagtctttgg gtgattggaa ctatccggaa actccggata gttcgcggaa gcccgcagca
      601 acccgcgcca gtgctcacaa gctcgggagg ccagtcggct catggtcaac tgtccgccac
      661 cattcatctt gcgaggctgt tcactgattt ttcagtgaac agccttttct ctttcaggct
      721 tcgcttcagt ccaccagccc cgtcaccgga atcgccgcgc cgtggatggc ggcggcgcca
      781 tcggatgcca ggaaggcgat cacatcggcc agggcggccg gggccaccca gcgcgacgcg
      841 tcggcgtctg gcatggcggc gcggttgtcg ggtgtgtcga tgatggaggg catgacgcag
      901 ttgacgttga tgccctggtg cttcaattcg gccgacatcg attcggtcag gcgaatgagc
      961 gcgctttttg acgcggcgta ggcgcccatg tgggcgcccc ctttcaacgc cgcgccggcg
     1021 ccgatggtga cgatgcggcc gcggccgcgc tggatcatgg ccggcacaac cgcgctggcg
     1081 acgttgatca aggtgcgggc gttcaagtcc atgaggaagt cccaggtggc ggcactggtt
     1141 tcatgcaccg actcgcccat gcgaaagccg ccggcgatgt ggcagcaggc gtcgatggcg
     1201 ccaaagcggt cgagcgcctg ctgaacgccg gcttgcacgc catcccgatc catgagatcg
     1261 atcggcagca gcaagacgtc gtctgcgtgg tcgaacgccg ccttcaacgc gtccaggcgt
     1321 cgatccgcca gcaccaggcg cgcgccacgt tgccgaaaag tggcggctac ggcctggccg
     1381 agatggccgg cggcgccggt gatgagcacg gtctgcggcg cgctcatgca cccgctccgt
     1441 cgcgcttggc ggcgtggtag cggccgctgc cgcgcgtcag cacggtgtcg gtgggcagga
     1501 tgtcgcccgg ctcgaagtcg cttgcgtcgg ccacgcgctc gtacagcggc gcaaagtcga
     1561 tgcgcgccag ttccagcagg tcgttgaggt tgtccagcac gaagtaggtt tcctggaagt
     1621 cgtcgatgcg gtagttggtg cgcatcacgc gctccagatc gaaggcaacg cggttcggtg
     1681 agtcgtcctc gacggagaaa acggtttcgg tggcggacga tgcaatgcct gcgccataga
     1741 cgcgcaggcc gtccggctgc ttcaccagcc cgaactcgac cgtgtaccag tagacgcgcg
     1801 ccagcttctc cagcacgccc aggcgcttgg cgcgcaggcc gccttcgcca taagcctgga
     1861 tgtagtcggc aatcgccggg ttcatcagca tcggcacgtg gccgaacacg tcgtggaaca
     1921 cgtcgggctc ttccaggtaa tccagctcgt gcggtttgcg gatgaagtgg ccggccggaa
     1981 agcggcggtg cgccaggtgt tcaaagaaca cgtcgtcagg caccagcccc ggcaccgcga
     2041 cgatctgcca gccggtgcgc tggctcagcg tttcagacaa ctgctcgaag ttgggaattt
     2101 gatccgggcc aatcggcaaa tcctgcatgc cgcgcacgaa ctcgtcgcag gcgcggccgg
     2161 gcagcagctt gctctggcgc tcgaacagcg tcttccacac cgcgtgttcc tgcgccgtgt
     2221 actggctcca gccctggtcg atggtccagt cgggccgctc ggggcgttga tcggtgccgg
     2281 cggccaggcc gtgtttaacg tgttccttga gctgctgcag catgctttgg tctcctgcct
     2341 tcaaaccgct tgctgggcca gcgcggcggc gacgcggtcc atcagccgtg ccatgtcgat
     2401 gtcgttcatc gacaggccgc cgacgtcgtg cgttgtcagc gtgacggcaa cgcggttgta
     2461 gacgttgaac cactccgggt gatggttgcg cttttcggcg tgcaccgcga tctggcccat
     2521 gaagccgaac gcctgcgcga agtcgtgaaa gacgaattcg cgcttcagga tgccgccgcg
     2581 ttcatcgtgg tgcgtccatt gcggcaaatc cgccagcaag ggttggagct gggtcgcgtt
     2641 gagcttcatg gggtcacctt ttgagcgtga atcgggcaca aggcaacgaa tttagatgcc
     2701 aatgcccgca aacatcctgc gaatcatggc aaaaaaaccg gagcgcgcag gcatcgcgct
     2761 tgccagcgct gcgcggcgcc tgccggccac cttcacaggc cgtaaaagcg ccggatgatg
     2821 tcccaggcgt cttcgggcga atcggccatc tggtacaggt ccaggtcttc cagcgcgatg
     2881 gcgccttcat cgaccagcat ctgcagattg agcaggcggc gccagtagtc gctgccgaac
     2941 agcacgatgg gcaccttttg cgccttgcgg gtctgcacca gcgtcagcac ctcgaacaac
     3001 tcatcgagcg tgccaaagcc gcccggaaac gccaccagcg cccgcgcccg catcaaaaaa
     3061 tgcatcttgc gcagcgcgaa gtaatggaac ttgaaggcca gttgcggcgt gacgtagggg
     3121 ttggtttcct gctcgtgcgg caacgcgatg ttcaggccca ccgacggcac accgacgtcg
     3181 tgcgcgccgc ggttggccgc ttccatcaca ccggggccgc cgccggtgca gatgaacatc
     3241 ttttcttcct gcggctggcg caggctgtag gtggcgacga tgcggccgaa ctcgcgcgcc
     3301 tgcgtgtagt agcgcgaggt gcgcatggcg cgctcggcca gcgccatcgc ctgcgcatcg
     3361 ccgccggcgc gcgcgctggc cagcaactgc tcggccacgt cgggcgcgac gaagcgcgca
     3421 ccgccgtaca ccacgatggt gttgtggatg ccggcctctt gcaggcccag ctcgggcttg
     3481 agcagttcca gctgaaagcg gatgccgcgc acttcgcggc gcagcagaaa ctcagggtcg
     3541 gcaaaggcca ggcggtaggc atcgggctcc agcggctcgc cgcggtcggc gtgcgcctgc
     3601 aactcggccc aggcgttggc caggggggat tgggtggact gcttgatgtc ttgcgtcgta
     3661 tccatggtcg ctattgtatt aagagctgct cgcgcttgtc tgacaagcgc cggagacgaa
     3721 aaaggccccg aaactcggag cctttcttgc gcacggcggt cggctcaaag ccggcggcct
     3781 ttacacgcgc ttgcggtatt cgccggtgcg cgtgtcgatc tcgatcttgt cgccttgcgc
     3841 cacaaacagc ggcaccgcca cttcgtagcc ggtggcgatc ttggcgggct tgagcacctt
     3901 gcccgaggtg tcgcccttga cggccggctc ggtccaggtg atttcacgct cgacgctggt
     3961 cggcagctcg accgagatgg ccttgccgtc gtagaacacc acttcgacgg ccatgccgtc
     4021 ttcgaggtag ttcaacgcgt cgcccatgtt ctcggcttcg acttcgtact ggttgtactc
     4081 gccgtccatg aagacgtaca tcgggtcggc aaagtaggag taggtgcatt ccttgtggtc
     4141 gaggatgacc tgctccatct tgtcgtcggc cttgaagacg acttcggtgc ccatgttgtt
     4201 gagcagcgct ttcagcttca tgcgcacggt ggcggcgcct cggccgccgc gggcgtattc
     4261 ggtcttcagg acgatcatcg gatccttgcc gtgcatgatg acgttgccgg cgcggatttc
     4321 ctgggcgatt ttcatggtgt ggtgttccag tgtggggttg agcgtggccg ctcgtgcacc
     4381 gggttttgag tggcgaggcg cgctgcggcg aatggcgaaa cgtcgagccg accatgcagc
     4441 cggcgatttt tcgcaaagcc ttgaattcta acgcttttgg gcgacgaagc cgatcagctg
     4501 cgtgcccaag tcgggctgct ccagcaggcg ctggcgggcc gcttggacgc aggcgcgcca
     4561 gccgagcggg tcgatttcgg gcagcggacc ggcttcgata ccgttccaca cgtggtgaaa
     4621 gcggcgcagc gaggcgggcg cctgcagcca gtcgagaaag gcacccagct tggcgtggtg
     4681 cgcgtcgtct tcgggctgcg ggtagatgtg ccacaccagc ggcgcgccgg cccacagcgc
     4741 gcgcaccagc gaatcctcgc cgcgcacgaa gttgaggtcg cacgcccaca gcagttcgtc
     4801 gtaggcgcgc tgcgtcaaat ggggtaagta tgaaagtgat agctgctcgc gctggccaga
     4861 caagcgctgg aggtcatttt catcttcaag acagcgtgcc acggcgcgcg ccgcgcggcc
     4921 ggcggtgacg agcaggcgcg tgggcgtcgt ggccgtgtcc agctgccgca gcagcgcgcc
     4981 gagcgggggc ggctcgtagc agaacagcga ggcgatccgc tcgccacgcg catcaatgcc
     5041 ttgtgcttgc agccacgcgg cgccatcgaa cgcttgctgg cgcgcggcca agtcctgctc
     5101 gcgcaacagg ccgccagtgt gcggcgtgaa gccggggtag aaaaaccact tggtgcgccc
     5161 cgctgccggc ccgtgctgta cgggcgaggg cagggcgtgc atgcgctcga cccagggctc
     5221 ggcgctgagg tattccaggt tgatccaagt gggggcggtt cttgaatcaa atcggcctcc
     5281 agtgcttgag gagtcagcgc aggaggctat gaaatcagga gcaatatcgc agccgaaggc
     5341 ttcgatccag acatccgatg caggcagcga agccagcagg gcggggtcga tcggttgcgt
     5401 ccacggcagc acgcgcacgt cgggcacttc accttgcagc gcgccggacg ccatccagtg
     5461 cagcgcgctg gcatcgtcca cccacagccg cacctgctgc ccgcgcgccg ccaggtcgca
     5521 cgacaggcgc cagcacacgc ccagatcgcc gtggttgtcg atgacgcggc agaagatgtc
     5581 ccacagaagc ggcacggatt tgggcacgcg gcgattgtgc cccggggccg ccttcgtaaa
     5641 ctcgcggcca tgagccagcc gccctcagcg cccgaagccg gcgcgcccga gcccaccgac
     5701 gaacaaacgc ctgccgccac cggccacagc cccgagctgc tggcccaagt ggccgcgctg
     5761 cccggcctgc cgggcgtgta ccgctattac gacgccgccg gcaacgtgct gtacgtgggc
     5821 aaggcgcggc atttgaagaa gcgcgtgtcg agctacttcg tcaagaacca tggcggcacg
     5881 cgcatcggcc acatggtggg caagatcgcg cggctcgaca ccaccgtggt gcgctctgag
     5941 gccgaggcgc tgattcttga gaacaacctc atcaagcagc tggcgcccaa gtacaacatc
     6001 ctgttcaggg acgaca
//
LOCUS       DAOMVO010000139         5987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3563_length_5987_cov_15.457350, whole genome shotgun sequence.
ACCESSION   DAOMVO010000139 DAOMVO010000000
VERSION     DAOMVO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_5987_cov_15.457350"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..808)
                     /locus_tag="PKV30_07895"
     CDS             complement(41..808)
                     /locus_tag="PKV30_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30170.1"
                     /translation="MSTLSTQDLTIRFGGHVAVNAVTCSFEPGTLTAIVGPNGAGKTT
                     YFNLISGQLKASGGTVKLGGRDLSGLSPSARTKAGLGRAFQLTNLFPNLSVLENVRLA
                     VQAHLHDGLNLWSIWSDHRDLTRRAHEILESVALNDKQHMPVASLPHGDQRKLEVALL
                     MALDPQVYMFDEPTAGMSHDEAPVILNLIRALKQDKTKTILLVEHKMDVVRELADRII
                     VLTNGTLVADGDPAEVIASPVVQEAYLGVTKEDEVAA"
     gene            complement(946..2127)
                     /locus_tag="PKV30_07900"
     CDS             complement(946..2127)
                     /locus_tag="PKV30_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRL30171.1"
                     /translation="MQRRTLIALAALATAIGAPALAQSEIKIAHVYSKTGPLEAYGKQ
                     TQAGLMMGLNYATGGTMAVGGKKITVIEKDDQGKPDLGKSLLATAYSDDKVDLAVGPT
                     SSGVALAMLPVAEEYKKILLVEPAVADSITGDKWNKYIFRTGRNSSQDAISNAVAIDK
                     PGTTIATLAQDNAFGRDGVKAFKDAVKTAKLVHEEYLPAATTDFTAGAQRLIDKLKDQ
                     PGRKIIWIVWAGAGNPFKIADMDLKRHGIEIATGGNILPAMAAYKNLPGMEGAAYYYF
                     GIPKNPVNEAMVAAHYKEYKTPPDFFTAGGFSAAMAIVTALKATGGDTNTNKLIKTME
                     GMSFDTPKGKMTFRKEDHQAMQSMYHFKIKNDPAFAWGVPELVREIKPEEMNVPIRNK
                     R"
     gene            complement(2336..3832)
                     /locus_tag="PKV30_07905"
     CDS             complement(2336..3832)
                     /locus_tag="PKV30_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRL30172.1"
                     /translation="MDTPPARSCALPRDAASILELAARSMFDLFASASEGMLLVDREA
                     RVVWINDQYRRFLPALGFPREEDFVGHAVSDVVQNTQMHQVLRSGKPILIDLLSNRAG
                     TFVVSRIPLRDEAGEVIGALGIVLFDQASHNLQPLIAKFAQMQHDLHEAQRQLADKKA
                     ASAHGSRQSRYTFASFVGTSPAAVEVKRQARRAAQSGSPVLLLGETGTGKELLAHAIH
                     AASGRASGPFVSVNIAAVPDTLLEAEFFGVAPGAYTGAERKGRVGKFKLADGGTLFLD
                     EIGDMPPGLQAKLLRALQEGEIEPLGSNQLVPFDARVVAATSRDLPALVASGQFREDL
                     YYRLNVLPVRVPPLRERRGDIPALIEVLGEDMAHRGSPPPELSASAVALLAAQQWRGN
                     IRELRNVLEQAALRADSDRLDAGQMEALLRQSGLAQIQPALPPEPAPPDASALLRPLA
                     EQVAELEQRAIAAAMQTTGGNKLAAARLLGISRATLYERLDARQPRAA"
     gene            complement(3930..4199)
                     /locus_tag="PKV30_07910"
     CDS             complement(3930..4199)
                     /locus_tag="PKV30_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRL30173.1"
                     /translation="MNLIIWLIVGGILGWLASIVMKRDAQQGVLLNVVVGIVGAFLGG
                     FLLSPLLGAGTINMNDFSVMGLVVSFLGAVILLAIVNLFKRGSVR"
     gene            4371..4598
                     /locus_tag="PKV30_07915"
     CDS             4371..4598
                     /locus_tag="PKV30_07915"
                     /inference="COORDINATES: protein motif:HMM:NF021512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HRL30174.1"
                     /translation="MSGIASHLPQFEDASRALFAGDASAFDACIDACIAAWPGGVPAH
                     LRWLARDAFGELTSLRSMTSASPSVTRPAGA"
     gene            4747..>5987
                     /locus_tag="PKV30_07920"
     CDS             4747..>5987
                     /locus_tag="PKV30_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL30175.1"
                     /translation="MARPAVSAQDASRPLWRIYLAFLGPMVLANILQSLSGSVNAVYI
                     GQMLGTQALAAVAGMFPIVFFFIALIIGVGAGASVLIGQAWGAREPAKVKAIAGTALG
                     LGGLIGIAAAVLGELFARPVLQALGTPAEVLPDATAYARVMMLTMPLLLVFILYTQLL
                     RGVGDTLTPLYALMLSTGIGLVLTPALIRGWGGLPQLGVVSAAVAGLVSFAVALTFLV
                     IYLQRKRHPLAPDAALRRALWIDPALLKGVLRIGVPTGVQMVTISLAELAILGLVNRY
                     GADATAAYGAVNQIVNYVQFPALSIAITASILGAQFIGAGRADRLGALVKTGLLLNVL
                     LTGALIVAGYLLSPWLLGFFLTSEPVRQMAEHLLHLMLWGSLVFGFQAVIAGVMRASG
                     TVLIPVAISVGSILLVQLPAA"
BASE COUNT          896 a         1907 c         2046 g         1138 t
ORIGIN      
        1 ctcaccccaa ccctctccca gagggaaaaa taccggacgc tcatgccgcc acctcgtctt
       61 ctttcgtcac gcccaggtac gcttcctgaa ccaccggcga tgcgatcact tcggccgggt
      121 cgccatcggc caccagcgtg ccgttggtga gcacgatgat gcggtcggcc agctcgcgca
      181 ccacgtccat cttgtgctcg accagcagga tggttttggt cttgtcctgc ttcagcgcac
      241 gaatcagatt caggatgacc ggcgcctcgt cgtggctcat gccggcggtg ggttcgtcga
      301 acatgtagac ctgcggatcg agcgccatca gcagcgccac ttcgagcttg cgctggtcgc
      361 cgtgcggcag gctggccacg ggcatgtgct gcttgtcgtt cagcgccacc gattcaagga
      421 tctcgtgcgc gcggcgcgtg aggtcgcggt ggtcgctcca gatgctccac aggttcagcc
      481 cgtcgtgcag gtgcgcctga acggccaggc gcacgttctc cagcacgctc aggttgggga
      541 acaggttggt gagctgaaac gcccggccca gtcctgcctt ggtgcgcgcc gacggcgaca
      601 gccccgacaa atcacgcccg cccagcttta cggtgccgcc gctggccttg agctggcccg
      661 agatcaggtt gaagtaggtc gtcttgccgg cgccattcgg tccgacgatg gccgtcagcg
      721 tgccaggctc gaagctgcac gtgaccgcgt tcacggccac gtggccgcca aagcggatgg
      781 tcagatcctg ggtagatagg gtgctcatga ttcaatagct gcttgcgcag attgcacggg
      841 cgctggcggc actttgggtt tagattttga caacacgatc ccacctcagc gccccagggc
      901 gctgggattc ggttcaatgg tgtgaagtga acgaaacggc gtcgatcagc gcttgttgcg
      961 gatcggcacg ttcatctctt caggcttgat ctcgcgcacc agctccggca cgccccaggc
     1021 gaaggccggg tcgttcttga tcttgaagtg gtacatgctc tgcatggcct ggtggtcttc
     1081 cttgcggaag gtcatcttgc ccttgggcgt gtcgaagctc atgccttcca tggtcttgat
     1141 gagcttgttg gtgttggtgt cgccgccggt cgccttcagt gcggtgacga tggccatggc
     1201 cgccgagaag ccgccggcgg tgaagaagtc gggcggcgtc ttgtattcct tgtagtgcgc
     1261 ggccaccatg gcttcgttca ccgggttctt ggggatgccg aagtagtagt aggccgcgcc
     1321 ttccatgccg ggcagattct tgtaggcggc catggcgggc aggatgttgc cgccggtggc
     1381 gatctcgatg ccgtggcgct tcagatccat gtcggcgatc ttgaaggggt tgccggcgcc
     1441 ggcccagacg atccagatga tcttgcggcc cggctggtcc ttgagcttgt cgatcaggcg
     1501 ctgcgcaccg gcggtgaagt cggtggtggc cgcgggcagg tattcctcgt gcaccagctt
     1561 ggcggtcttg acggcgtcct tgaacgcctt cacgccgtcg cggccaaagg cgttgtcctg
     1621 cgccagcgtg gcgatggtgg tgccgggctt gtcgatggcc acggcgttgc tgatggcgtc
     1681 ctggctgctg ttgcggccgg tgcggaagat gtacttgttc cacttgtcgc cggtgatcga
     1741 atcggccacg gcgggctcga ccagcaggat cttcttgtat tcctcggcca ccggcagcat
     1801 ggccagcgcc acgcccgacg acgtggggcc gacggccagg tcgaccttgt cgtcagagta
     1861 ggcggtggcc agcaggctct tgcccagatc gggcttgccc tgatcgtcct tctcgatcac
     1921 ggtgatcttc ttgccgccca cggccatggt gccgccggtg gcgtagttca ggcccatcat
     1981 caggccggcc tgtgtctgct tgccgtaggc ctccagcggg ccggtcttgc tgtagacgtg
     2041 ggcgatcttg atttcgcttt gggccagcgc gggcgcgccg atggcggtgg cgagagcggc
     2101 gagggcgatc agggtgcggc gttgcatggg gtgtctcctg tcaatgcaaa tgagggggcc
     2161 tgcaccctta gcgcacagag cgtgccaacg tgcaagtgat tgatttaatt gacgggtcga
     2221 atgtgatcgc ccgctaagtg actgaaataa atacaaatta atcgcctgaa aatcaaacaa
     2281 atgatcgaaa tttgttcagg gtaaacccgc caacgcggat cgagcgaaag gccgttcagg
     2341 cagcgcgcgg ctggcgcgcg tccagccgct cataaagcgt cgcgcgtgag atgcccagca
     2401 gtcgcgccgc cgccagcttg ttgccgccgg tggtctgcat cgccgcagcg atggcgcgct
     2461 gctccagctc ggccacctgc tcggccagag ggcgcaacag ggcgctggcg tcgggcggtg
     2521 cgggttcggg cggcagcgcg ggctggatct gggccaggcc ggattggcgc agcagggcct
     2581 ccatttgccc ggcgtccaga cggtcggaat cggcacgcag cgcggcctgc tccagcacgt
     2641 tgcgcagctc gcgaatgttg ccgcgccatt gctgcgctgc cagcagggcc acggcgctgg
     2701 cgctcagctc gggcggcggg ctgccgcggt gcgccatgtc ttcgcccagc acctcgatca
     2761 gcgccgggat gtcgccgcgc cgctcgcgca gcggcggcac acgcactggc agcacattca
     2821 ggcggtagta caggtcttcg cgaaactggc cgctggccac cagcgccggc aggtcgcggc
     2881 tggtggcggc caccacgcgg gcgtcaaaag gcaccagctg gttgctgccc agcggctcga
     2941 tctcgccttc ctgcagcgcg cgcaacagct tggcctgcag gccgggcggc atgtcgccga
     3001 tttcgtccag aaacagcgtg ccgccgtcgg ccagcttgaa tttgccgacg cggcccttgc
     3061 gctcggcgcc ggtgtaggca ccgggggcga cgccgaagaa ctcggcctcc agcaaggtat
     3121 cgggcaccgc cgcgatgttg acgctgacaa acggcccgct ggcgcgcccc gacgcggcat
     3181 ggatggcgtg cgccagcagt tccttgccgg tgccggtttc gcccagcagc agcaccgggc
     3241 tgcccgattg cgccgcgcgc cgggcctggc gcttgacttc caccgcggcc gggctggtgc
     3301 ccacgaagct cgcaaaagtg tagcgagatt gccttgatcc atgtgcgctg gcggcctttt
     3361 tgtctgccaa ctggcgctgc gcctcgtgca aatcgtgctg catctgcgca aacttggcga
     3421 tcagcggctg caggttgtgc gaggcctggt cgaacagcac gatccccagg gcgccgatga
     3481 cttcgcccgc ctcgtcgcgc agcggaatgc ggctgacgac gaaggtgccg gcgcggttgc
     3541 tcagcaggtc gatcaggatt ggcttgccgc tgcgcagcac ctggtgcatc tgcgtgttct
     3601 gcaccacgtc ggacacggcg tggccgacaa agtcttcctc acgtggaaag cccagcgccg
     3661 gcagaaagcg gcggtactgg tcgttgatcc acaccacgcg cgcttcgcgg tcaaccagca
     3721 gcatgccctc gctggcgctg gcgaacaaat cgaacatcga gcgcgccgcc agttccagaa
     3781 tgctggcggc gtcgcgcggc agggcgcagg atcgggcggg cggggtgtcc atggcgagcc
     3841 ggcatggtag cgcgcccgcc cgcccatgaa aaaagccgcg cgaggcaagc ctcgcgcggc
     3901 tttttcgaga tgaagagtgt tcgcgtcgat cagcgcacgc tgccgcgctt gaacaggttg
     3961 acgatggcca gcaggatcac cgcgcccagg aaggacacga ccaagcccat gacggagaag
     4021 tcgttcatgt tgatggtgcc ggcgcccagc agcggcgaca gcagaaagcc gcccaggaac
     4081 gcgccgacga tgccgacgac cacgttcagc aaaacgcctt gctgcgcatc acgcttcatc
     4141 acgatgctgg cgagccagcc cagaatgcca ccgacgatca gccagatgat gaggttcatg
     4201 ttggttctcc gagtttgcga aaggtttgaa aaggtttcac gcgatccgac gtcttgtgtc
     4261 cgtcgtgaaa ccgattgttc agcccgcgcc ggccgcgcat cgtcagcccg ccgcgctcac
     4321 agttgtagga cgttgcaacg gcgcgttgac ggtgcgggga gtatcgtttc atgagcggca
     4381 tcgccagcca cctgccccag ttcgaggacg cttcgcgcgc gctgttcgcg ggcgacgcca
     4441 gcgcgttcga cgcctgcatc gacgcctgca tcgccgcctg gccgggcggc gtgccagcgc
     4501 acctgcgctg gctggcgcgc gatgcgtttg gcgagctgac ctcgctgcgc tcgatgactt
     4561 cggcttcgcc ttccgtgacg cgccctgcgg gcgcatgact tcagtcatgg cgcggtgcgc
     4621 cgtcatccaa ggcgcagcct tgggtcatgc gccgaaggcg cggtcatcgc tccataattg
     4681 atgaaacaaa aaggccgtca gcgcgtgctt atcttgcgcc agcagctatc acgaaagtag
     4741 tgaaccatgg caagaccggc ggtctctgcg caagacgcct cgcgcccctt gtggcgcatt
     4801 tacctggcgt tcctcggccc catggtgctg gccaacatcc tgcaatcgct gtcgggcagc
     4861 gtcaacgcgg tctacatcgg gcagatgctg ggcacgcagg cgctggcggc ggtggcgggc
     4921 atgttcccca tcgtgttttt cttcatcgcg ctcatcattg gcgtgggcgc gggcgcttcg
     4981 gtgctgatcg gccaggcctg gggcgcgcgt gagccggcca aggtgaaggc gattgccggt
     5041 accgcgctgg ggctgggcgg gttgatcggc atcgcggcgg cggtgctggg cgagttgttc
     5101 gcgcgcccgg tgctgcaggc gctcggcacg ccggccgagg tgctgcccga cgccacggcc
     5161 tacgcccgcg tgatgatgct gaccatgccg ctgctgctgg tgttcatcct ttacacgcag
     5221 ctgctgcgcg gcgtgggcga cacgctgacg ccgctgtacg cgctgatgct gtccaccggc
     5281 attggcctgg tgctgacgcc agcgctgatc cgcggctggg gcggcctgcc gcagctgggc
     5341 gtggtcagcg cggcggtggc cgggctggtg tcgtttgcgg tggcgctgac gtttctggtc
     5401 atctacctgc agcgcaagcg ccacccgctg gcgcccgacg ccgccttgcg ccgcgcgctg
     5461 tggatcgacc cggcgctgct caaaggcgtg ctgcgcattg gcgtgccgac cggtgtgcag
     5521 atggtcacca tctcgctggc cgagctggcg attctgggcc tggtcaaccg ctacggcgcc
     5581 gacgccacag cggcgtatgg cgcggtgaac cagatcgtca actacgtgca gtttccggcg
     5641 ctgtcgattg ccatcacggc gtcgatcctc ggcgcgcagt ttatcggcgc cggccgtgcg
     5701 gatcggctgg gcgcgctggt caagacgggc ttgctgctca acgtgctgct gaccggcgcg
     5761 ctgatcgtgg cgggctacct gctgtcgccg tggctgttgg gctttttcct gaccagcgag
     5821 ccggtgcgcc agatggccga acacctgctg cacctgatgc tctggggcag cctggtgttc
     5881 gggtttcagg ccgtgattgc cggggtgatg cgcgccagcg gcaccgtgct gatcccggtg
     5941 gcgatttcgg tcggcagcat cttgctcgtg cagttgccgg ccgccaa
//
LOCUS       DAOMVO010000140         5938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3605_length_5938_cov_16.368519, whole genome shotgun sequence.
ACCESSION   DAOMVO010000140 DAOMVO010000000
VERSION     DAOMVO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_5938_cov_16.368519"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKV30_07925"
     CDS             <1..743
                     /locus_tag="PKV30_07925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRL30176.1"
                     /translation="DLVLDSFLGSGTTAAVAHKMGRRWIGIEMGEHARTHCLPRLEKV
                     IAGEGGGISRAVGWGQPAADGTPWNAAQQGGGFRFMRLGEPIFDAAGHIHPAVRFATL
                     AAFVWQQETGRAYDPQDPTTGARPGTPLLGIHYENMSCPRLPDERESPFSSESRETAP
                     STPAPPQPRPRAAIYLLFNGILGDKRPDSGNVLTRDVLAALLALHAQSPAPQAPLIVY
                     GEACRLGPARLAQARVTFRHIPHDVRAG"
     gene            749..2482
                     /locus_tag="PKV30_07930"
     CDS             749..2482
                     /locus_tag="PKV30_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRL30177.1"
                     /translation="MTTHDMRAWLDELRALPRETEWLEFKRAEKSFSFEQMAQYVSAL
                     ANEANLARREEGWLVMGVEDRIDPASGLRPASGTQFATSAEEINRVKESIAAQTAPPV
                     TLETPTEVRVPGADSQLARVLLWRIPAAPRGMPVACKGHWWGRAGEKLGALAVHKLDT
                     LRVQSAMHDWSAGLVTDDWTLLEPAAIARARELFARRYASKPDLVSQMRSQSDVEFLH
                     CLRLAVDDRLTRAALVLLGRSVAASHLGGATPRIGWQLVDHRGDHLTHQHFELPLVLG
                     IDALIARIRIIEVNLLPPREVAPLNLPNYDDWVIREALHNCVAHQDYAQGGRVLVTES
                     PDTLRFFNYGEFLPGTLERVLLARQPEQRYRNACLANAMVELDLMETMNRGVKGMFRK
                     QRERFFPLPDYELTAQPATVSVTIHGRTLDERYVQALMLNTDLSLEDAILLDRVQKAR
                     SVPSAALQRLRGRGLIEGRGKKTRISAAVAVATGTEVAYVNQKGPEAQDYKDAVCRLL
                     ALTPQPRSKIDDLLLPKLAMWIPDAPKRKEYVKGLLAEMARQGTIRNIGKPTRGALWA
                     LIAPMSAKENQ"
     gene            2583..5273
                     /locus_tag="PKV30_07935"
     CDS             2583..5273
                     /locus_tag="PKV30_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRL30178.1"
                     /translation="MPTFPLKSYQQAALDTLTAFTQAAQVKGAPLAFGELAGRAYNPD
                     PFGPDVPCVCLRIPTGGGKTLLAAHAVPLLARHWRASDAPVAVWLVPSETIRAQTLTA
                     LQTPGHPYRQPLADAYGDALRVVALDELAQIAPPDWGRAAVVVVATIQSFRIDDTDKR
                     NVYSFSERFERHFKAAESAQPRDLAALRDLPDAVVTEGEAAQDGRALLRGFVGEPRQS
                     LANWLALQRPIVIVDEAHNTKTDKSFTALKRLNPACLLELTATPIAARTNVLYHVSAQ
                     ELAAESMIKLPIALAEHPEGWPQAVFGAVQTQRALEAAALKDEAAGTQYVRPIVLFQA
                     QNEGQAVPPEALRRHLIDELHLPAEQIVVATGSTRELDGLDLQRRDCPVRFVITVQAL
                     REGWDCPFAYVLCSLQALSSATAVEQLLGRVLRMPYATRRGREALNRAYAHVCAAEFS
                     SAAHALADRLINGMGFEALDVASMLAPAASLPLFEENQAPALSQQALIATNFEAITPT
                     DELTALPGVHIHTIAGAPQVVVQGHIGSELEAKLLTGVRGAKKQESVREQIAQHNALV
                     AAQTAPAARGAVFLPVPPLAYRDDSQGELWPLEREALLESVEIDLLTPAAIDLPGFQV
                     VQQSHLFEIYLKDEHVKLRPADAAQMAMDYGSSSITEQDLVRWLDQSLYQKLPWLTAG
                     QRGAWFAAVVRRLVHERGVPLVTLAQARFTLARALEAKADDLRDAAAARQFRQQVLGG
                     GWRVEPDWARPHVFEPGRYPAPAGSRYAGRYEFRKHYYPVLADLKDGGEEFLCAQLID
                     LHPKVRHWVRNLASAPAGFALPTSRGWFYADFVAELTDDRVALLEYKGAHLANDPGEI
                     EKRQIGELWARQSDGKAVFGWLMLTQGGKSLAQQLDEVLV"
     gene            5282..>5938
                     /gene="lipB"
                     /locus_tag="PKV30_07940"
     CDS             5282..>5938
                     /gene="lipB"
                     /locus_tag="PKV30_07940"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRL30179.1"
                     /translation="MRMLGRVDYAPTFQAMKDFTAQRTPDEREQLWVCEHSPVFTLGL
                     AGKPEHVLNAGDIPVVQTDRGGQVTYHGPGQVVAYPLIDLRPRGIFVKEYVYRLEEAV
                     IRTLAHFGVTGHRVGGAPGIYVRLDDPFSHAVLLQKPQLRAPGSPAPEPDFTGLGKIA
                     ALGIKVSNHRAYHGVALNAAMDLEPFARINPCGYAGLKTVDLSTIGVSASWDEAAHEL
                     A"
BASE COUNT         1044 a         1919 c         1993 g          982 t
ORIGIN      
        1 gcgacctcgt cctcgacagc ttcctcggct ccggcaccac ggcggcggtg gcgcacaaga
       61 tggggcggcg ctggatcggc atcgaaatgg gcgaacacgc ccgcacgcac tgcctgccgc
      121 ggcttgaaaa ggtcatcgct ggcgaaggcg gcggcatcag ccgcgccgtc ggctggggcc
      181 agccggccgc cgacggcacg ccgtggaacg ccgcgcaaca aggcggcggc tttcgcttca
      241 tgcgcctggg cgagccgatc ttcgacgccg ccggccacat tcaccccgcc gtgcgctttg
      301 ccaccctggc cgccttcgtc tggcaacagg aaaccggccg cgcctacgac ccgcaagacc
      361 ccactaccgg cgcccggccc ggcacgccgc tgctcggcat tcactatgaa aacatgagct
      421 gcccgcgctt gccggacgag cgcgaaagcc ctttttcatc agaatcccgt gagaccgcgc
      481 ccagcacccc tgcgccgcca cagccccgcc cacgcgccgc catctacctg ctgttcaacg
      541 gcatcctggg cgacaagcgc ccggacagcg gcaacgtgct cacccgcgac gtgctcgccg
      601 ccctgctggc cctgcacgcc caaagccccg cgccgcaagc cccactcatc gtctatggcg
      661 aagcctgccg cctggggccc gcccgtctgg cgcaggcgcg cgtgacgttc aggcacattc
      721 cgcatgatgt gcgggcgggg tgagccagat gacgacgcat gacatgaggg cttggctgga
      781 tgaattgcgc gccctgccgc gcgagacgga gtggctggag ttcaagcgcg ctgaaaagtc
      841 gttcagcttc gaacagatgg cgcagtacgt gtcggcactg gcgaatgagg cgaatctggc
      901 ccggcgcgag gagggttggc tggtgatggg cgtggaggac cgcatcgacc cagccagcgg
      961 cctgcgcccg gcatctggaa cccagttcgc gacgagcgcg gaggagatca accgcgtcaa
     1021 ggaatccatc gctgcgcaaa ccgcgccgcc ggtgactttg gaaacgccga cggaagtgcg
     1081 cgttccaggg gccgacagcc agctggcgcg cgtgctgctg tggcgcattc ccgccgcgcc
     1141 gcgtggcatg ccggtggcgt gcaagggtca ttggtggggc cgcgcgggcg agaagctggg
     1201 cgcgctggca gtacacaagt tggatacctt gcgcgtgcag tcggcgatgc acgactggtc
     1261 tgccggcctg gtaacggacg actggacgct gctggagcca gcggccatcg cccgtgcgcg
     1321 tgaactgttt gcgcgtcgat atgcctcaaa accggatttg gtgtcgcaga tgcggagcca
     1381 atcggatgtg gagtttctgc attgcctgcg ccttgcagtc gatgatcgcc tgacgcgtgc
     1441 ggcgctggtt ctgctgggcc gttccgtggc tgcgtcgcat ctgggtggcg ccacgccgcg
     1501 cattggttgg cagttggtcg atcatcgagg tgatcattta acgcaccagc atttcgagtt
     1561 gccactggtc ttgggcattg acgccctgat cgcgcgcatt cgcatcatcg aagtcaactt
     1621 gttgcctccc cgcgaggtgg cgccgctgaa cctgcccaat tacgacgatt gggtgattcg
     1681 cgaagccctg cacaactgcg tggcacacca ggactacgcc cagggtggcc gggtgcttgt
     1741 cacggaaagc cccgataccc tgcgattctt caattacggt gaattccttc ccggcacgct
     1801 ggaaagggtg ttgctggcgc gtcagcccga gcaacgttac cgcaacgcct gcctagccaa
     1861 tgccatggtg gaactggatt tgatggaaac gatgaaccgg ggcgtgaaag gcatgttccg
     1921 caagcagcgc gagcgcttct ttccactgcc ggactatgag ctgacagcgc agccagcgac
     1981 cgtgagcgtg accattcatg gccgcacact ggatgagcgg tacgtgcagg ctttgatgct
     2041 caatacagac ctcagcctgg aagacgcaat cctgctggac agagtgcaaa aggcgcgttc
     2101 tgtgccgtct gcggcattgc agcgcctgcg cggccggggc ttgattgaag ggcgcggcaa
     2161 aaaaactcga atttcggccg ccgtggccgt tgccaccggc accgaggtgg cttacgtgaa
     2221 tcaaaaaggc ccagaggcgc aggattacaa ggacgcggtg tgccggcttc tcgcgctaac
     2281 gccgcagcct cgcagcaaga ttgatgactt gcttctgccc aaactcgcca tgtggattcc
     2341 agacgcgccc aagcgcaaag agtatgtcaa gggactgctc gcagaaatgg cccgacaggg
     2401 caccatcagg aacattggca aaccaacaag gggcgcgctg tgggcgctga ttgcgccaat
     2461 gagcgccaaa gaaaaccagt aaatccacca atcactcaaa aaaacctcaa ttaaatcaac
     2521 aacttgcatt ggcgaaacga aagctcgaat cagtctttgc tccaatccaa gtcctcttgg
     2581 ccatgcccac cttccccctc aagtcctacc agcaagccgc cctcgacacc ctcacggcct
     2641 tcacccaggc cgcgcaggtc aagggcgcgc cgctggcgtt tggcgagttg gcagggcggg
     2701 cgtacaaccc cgacccgttc gggccggatg tgccgtgcgt gtgcttgcgc attcccaccg
     2761 gcgggggcaa gacgctgctg gcggcgcatg cggtgccgct gttggcccgc cactggcgcg
     2821 ccagcgacgc gccagtggcg gtgtggctgg tgcccagcga gacgatacgc gcgcagacgc
     2881 tcacggcgct gcaaacgccg ggccacccgt atcgccagcc gctggccgat gcctatggcg
     2941 atgcgctgcg cgtggtggcg ctcgatgaac tggcgcagat tgccccgccg gactgggggc
     3001 gcgcggccgt ggtggtggtc gccaccatcc agagttttcg catcgacgac accgacaagc
     3061 gcaacgtgta cagcttttcg gaacgcttcg agcggcattt caaggcggcg gaaagcgcgc
     3121 agccgcgtga tctggcggcg ctgcgcgact tacccgacgc cgtggtgacc gaaggcgaag
     3181 cggcgcagga tggccgcgcc ttgctgcgcg gctttgtcgg cgagccgcgc cagagcctgg
     3241 cgaactggct ggcgctgcag cggcccatcg tcatcgtcga tgaggcgcac aacaccaaga
     3301 cggacaagag ctttaccgcg ctcaagcggc tgaaccccgc gtgtctgctg gagctgacgg
     3361 ccacgccgat tgcggcgcgc accaacgtgc tctaccacgt cagcgcgcag gagctggcgg
     3421 ccgaaagcat gatcaagctg ccgattgcct tggccgagca ccccgaaggc tggccgcagg
     3481 cggtgtttgg cgccgtgcag acgcaacgcg cgctggaggc cgcggcgctg aaggacgaag
     3541 cggcgggcac gcaatacgtg cgccccatcg tgctgtttca ggcgcagaac gaggggcagg
     3601 ccgtgccgcc cgaagcgctg cgccggcatt tgatcgacga gttgcacttg cctgccgagc
     3661 agatcgtggt ggccaccggc agcacgcgcg agctagacgg gctggatttg cagcggcgcg
     3721 actgcccggt gcgctttgtc atcacggtgc aagcgctgcg cgagggctgg gattgcccgt
     3781 ttgcctatgt gctgtgctcg ctgcaagcgc ttagcagcgc gacagcggtg gagcagttgc
     3841 tgggccgcgt gctgcgcatg ccctacgcca cacggcgcgg gcgcgaggcg ctcaaccgtg
     3901 cttatgcgca tgtgtgcgcg gcggaatttt ccagcgcggc gcatgcgctg gcggatcggc
     3961 tgatcaacgg catggggttt gaggcgctgg acgtagcctc gatgctggcg ccggcggcat
     4021 cgctgccact gtttgaagaa aatcaggctc cagcgcttag ccaacaagcg ctgatagcta
     4081 ccaatttcga agcaatcacc cccacggacg aactgaccgc gctgcccggc gtacacatcc
     4141 acaccatcgc cggcgcgccg caggtggtgg tgcagggcca catcggcagc gagcttgaag
     4201 cgaagttgct gaccggggtg cgcggcgcca agaagcagga atccgtgcgc gagcagattg
     4261 cgcagcacaa cgcgcttgtg gccgcgcaaa ccgcgccggc ggcgcgcggc gcggtgtttt
     4321 tacccgtgcc gccgctggcg tatcgggacg actcgcaagg cgagctgtgg ccgctggagc
     4381 gcgaggcgct gctcgaatcg gtcgagatcg acctgctcac gcccgccgcc atcgacctgc
     4441 ccggctttca ggtggtgcag caaagccacc tgttcgagat ttacctgaag gacgagcacg
     4501 tcaagctgcg cccggccgat gcggcgcaga tggcgatgga ctacggcagc agcagcatca
     4561 ccgagcaaga tttggtgcgc tggctcgacc aatctctgta tcagaagctg ccgtggctga
     4621 cggcgggcca gcgcggcgcg tggtttgccg ccgtggtgcg ccgcctggtg catgaacgcg
     4681 gcgtgccgct cgtgacgctg gcgcaggcgc gcttcacgct ggcgcgggcg ctggaagcca
     4741 aggctgacga tttgcgcgac gcggcggccg cgcggcagtt ccgccagcag gtgctgggcg
     4801 gcggctggcg ggtggagccc gactgggcgc gtccgcacgt gttcgagccg ggccgatacc
     4861 ccgcgcccgc cggcagccgc tacgccgggc gctatgaatt tcgcaaacac tactacccgg
     4921 tgctggccga cttgaaggac ggtggcgaag aatttctatg cgcgcaattg atcgatttgc
     4981 accccaaggt gcggcactgg gtgcgcaacc tcgccagcgc gcccgccggc tttgcgctgc
     5041 ccacttcgcg cggttggttc tacgccgact tcgtggccga gttgaccgac gaccgcgtgg
     5101 cgctgctcga atacaagggc gcgcacttag ccaacgaccc cggcgagatt gaaaagcgcc
     5161 agatcggcga gttgtgggcg cgccagagcg acggcaaggc ggtgttcggc tggctgatgc
     5221 tgacgcaggg cggcaagagc ctggcgcagc aactggatga ggtgctggtg tgagcctgga
     5281 catgcgcatg ctaggccgcg tggactacgc gcccaccttc caggccatga aggatttcac
     5341 cgctcagcgc acgccggatg agcgcgaaca gctatgggtt tgtgagcatt caccggtttt
     5401 cacgctcggc ctggccggca agcccgagca tgtgctgaac gccggtgaca tccctgtggt
     5461 gcagaccgac cgcggcgggc aggtgactta tcacggcccc ggtcaggtgg tggcctatcc
     5521 gctgatcgac ctgcgcccgc gcggcatctt cgtcaaggaa tacgtgtatc ggctggaaga
     5581 agcggtgatc cgcacgctgg cgcacttcgg cgtgaccggg caccgcgtgg gcggcgcgcc
     5641 cggcatttac gtgcggctgg atgacccctt cagccacgcc gttctgctgc aaaaaccgca
     5701 gctgcgtgcg cccggcagtc ctgcgccaga gcccgatttc accggtctgg gcaagatcgc
     5761 agcgctgggc atcaaggtca gcaaccaccg cgcctaccac ggcgtggcgc tgaacgcggc
     5821 gatggacctg gagccctttg cgcgcatcaa cccttgtggt tatgcggggc tgaaaacggt
     5881 cgatctttct acaatcggcg tttcggcttc atgggatgag gccgcccacg aactggcc
//
LOCUS       DAOMVO010000141         5934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3607_length_5934_cov_17.132165, whole genome shotgun sequence.
ACCESSION   DAOMVO010000141 DAOMVO010000000
VERSION     DAOMVO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_5934_cov_17.132165"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..271)
                     /locus_tag="PKV30_07945"
     tRNA            complement(196..271)
                     /locus_tag="PKV30_07945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Arg,seq:ccg)
     gene            complement(290..943)
                     /locus_tag="PKV30_07950"
     CDS             complement(290..943)
                     /locus_tag="PKV30_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRL30180.1"
                     /translation="MARRIKQDAQATREALLDAAERVFELRGVSHTSLAHIAEAAGLT
                     RGAVYWHFKDKAALFNAMMERVTLPLEKELEGLAQCTGDPIDALVQRLMHSIAQIASD
                     ARTQRVLHIATMMVEHVEDLWPVRERHAQVDSANVERVMQAFEAASALREQALPAPAR
                     QLADGFHALVRGLVHSWLLRRGFDLEATARVAVRAYLLGIGLPVHGDAAVAPARNQG"
     gene            1152..2435
                     /locus_tag="PKV30_07955"
     CDS             1152..2435
                     /locus_tag="PKV30_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL30181.1"
                     /translation="MQTSAPRRSMAFRLVPLGTSVLLAALLAACGDKQQPAAGGAGGG
                     APPPPEVAVVTVQPGTVKLSTELPGRLEPSRVAQVRARATGILNKRVFTEGSDVRAGQ
                     VLYQIDSAPYQATQQSAQAQLAQAQAQMANASAMVTRYRPLVAANAVSKQEFDAAVAA
                     EKAAQAQVAAARAAVRTADISLGYATVTAPISGRIGRSLVTEGALVSATEGTQLATIQ
                     QINPLYLNITQSAAEVLKLREALQAGKLARASGAEAARVQVLLENGQVYGQPARLLFT
                     DLSVDPTTGQVNLRAEVPNPGGLLMPGMYVRARVEQAEIANAILLPQQAVTRGTKGDS
                     VMVVAPDGAVAPRPIKIGGQKGQDWIVTEGLKPGEQVMVEGAMKLMMGAKVVKPVPWV
                     PKTASAQAQQAPAATKPAASAPAAAASAPASAASN"
     gene            2455..5628
                     /locus_tag="PKV30_07960"
     CDS             2455..5628
                     /locus_tag="PKV30_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL30182.1"
                     /translation="MARFFIDRPIFAWVIALFIIVLGATSITRLPVAQYPSVAPPTVV
                     LSAAYPGASAQTLEDSVLSVIEREMNGAPGLAYMESVAQANGTGSITLSFEPGTNLDL
                     AQVEVQNRLARATPRLPSVVNQQGVRVEKSQSNFLMFTMLSSSEPGADVTRIADYAAR
                     NVQPELQRLPGIGQVQLFGAERAMRIWLDPAKLQSYGLTPADVNAAIAAQNAQVSAGS
                     IGDLPLAPDQATTATVIVPGQLSTVEQFGNITLRAQTGGAAVRLKDVARIELGGQSYA
                     TAARLNGQPSVGMGIQLAPGANALAVAKAVKKRMAELEAFFPAGVSYTIPYDSSTFVQ
                     ISIEQVVHTLFEAVALVFIVMFIFLQNWRYTLIPTIVVPVALLGTLAVMLVAGFSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLSPVQATRKAMGQISGAIIGITVVLI
                     VVFVPLAFFSGSIGNIYRQFSLVMAVSIAFSAFLALSLTPALCATLLKPVEAGHHHEK
                     KGFFGWFNRGFTRLTSRYEGWVGALLKRGGRMMIVYLAIGAAVGWMYTRLPTSFLPQE
                     DQGYIIVNIQLPAGATLERTQAVVEQVEGYLLKQPEVANMVAVMGFSFSGQGQNAALS
                     FVSLKPWDERKGAEHGADAVAGRAMGALSGVKDAFIFSLSPPPIPELGVSAGFNLRLQ
                     DRSSQGHDALLAARNQLLGMTAQSKVVSGVRPNGLEDAPQLRVDINRDQAAAQGVSFS
                     AINSAISTALGSSYVNDFPNAGRMQRVVVQADAAARMQPEDILRLTVQNSAGQAVPLS
                     AFATTSWVTGPMQTVRYNGYPAMRIEGAAAPGQSTGQAMAEMEALAAKLPPGFGIEWT
                     GQSREEKLAGNQAILLYAFAILAVFLALSALYESWSIPLAVILVVPLGVLGVLIGVNM
                     RGLTNDVYFQIGLITIIGLSAKNAILIIEFAKDLQAQGRSVLQAALEAAHLRFRPILM
                     TSLAFTAGVVPLAIASGASSASQRAIGTGVIGGMVVGTVLSVFLVPVFFLVVRSIFKG
                     GKREQEMAQQHARSAGVGSGEPVEPTP"
     gene            5655..>5934
                     /locus_tag="PKV30_07965"
     CDS             5655..>5934
                     /locus_tag="PKV30_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HRL30183.1"
                     /translation="MKARALVRWLATPLCVATLAACSFVPKYEQPAAPVSATWPDSAG
                     AQVEGGVAAADLPWQQFVHDAQLREVIQLALENNRDLRVAVQNIEQARA"
BASE COUNT          975 a         2056 c         1984 g          919 t
ORIGIN      
        1 agacaagcgc gagcagctat caaaaacaca gcggccaacg aaaaaagcct tggcgccagc
       61 caaggctttt gcagcgtgat caaaaggccg cggagcaggc catccgggaa cgccgtggtc
      121 ggggtccccc cgaccaccag cgttgtcccc ctccggggga aggcgcgtca gcgcctcagg
      181 ggggagcgta aagtctggcg cggctggcag ggatcgaacc tgcgaccctt ggcttcggag
      241 gccaatactc tatccactga gctacagccg cttgcagaac agccggcgat tatccctgat
      301 tgcgcgcggg cgccaccgct gcatcgccgt gcaccggcag gccgatgccc agcagatagg
      361 cgcgcacagc cacgcgcgcc gtggcttcga ggtcaaaacc gcgccgcagc aaccagctgt
      421 gcaccagtcc gcgcaccagc gcatgaaagc cgtcggccag ttgccgcgcg ggcgccggca
      481 gcgcttgttc acgcagggcg ctggcggcct cgaaggcctg catcacgcgc tcgacattgg
      541 cgctgtcgac ctgcgcatgg cgctcgcgca ccggccacaa atcctcgaca tgctcgacca
      601 tcatggtggc gatgtggagc acgcgctggg tgcgcgcgtc gctggcgatc tgcgcgatgg
      661 agtgcatcag gcgctgcacc agcgcgtcga tcgggtcgcc ggtgcactgg gccagccctt
      721 ccagctcttt ttcaagcggc agcgtcacgc gctccatcat ggcgttgaac agcgccgcct
      781 tgtccttgaa atgccagtac accgcgccgc gcgtcagccc cgccgcctcg gcgatgtggg
      841 cgagcgaggt gtgcgacacg ccgcgtagct cgaacacgcg ctcggcggcg tcgagcaagg
      901 cttcgcgcgt ggcctgggcg tcttgcttga tgcggcgcgc catggtgtca gcaggaaatg
      961 ggctggatcg acatcagagt cagcactcta acacatacat tcacgcatgt atgtataatt
     1021 cgccgctttg ggcgcgtgtc cgcaagggat tttgactacg atcattcccc ggcgttgtga
     1081 tctgtggctg tttctggccg cacggcctgg tgcccgcaac ccgtgcccgc gtccctcagc
     1141 gagaaaagaa catgcaaacc tccgctccgc gccgctcgat ggctttccgt cttgttcccc
     1201 tgggcaccag cgtgctgctg gcggccctgc tggccgcctg cggtgacaag cagcaacccg
     1261 ccgccggcgg cgctggcggc ggcgccccgc caccgcccga agtggcggtg gtcaccgtgc
     1321 agcccggcac cgtgaagctg agcaccgaac tgcccggccg gctggagccc tcgcgcgtgg
     1381 cccaggtgcg cgcgcgcgcc accggcatcc tgaacaagcg cgtgttcacc gaaggcagcg
     1441 acgtgcgcgc gggccaggtg ctgtaccaga tcgattcggc cccttaccaa gcgacgcagc
     1501 aaagcgcgca ggcgcaactg gcacaggcgc aggcgcaaat ggccaacgcc agcgccatgg
     1561 tcacccgcta tcgcccgctg gtggcggcca atgcggtgag caagcaggaa tttgatgcgg
     1621 cagtggccgc cgaaaaagcc gcgcaggcgc aggtggccgc cgcgcgcgcc gccgtgcgca
     1681 cggccgacat cagcctgggt tatgccaccg tcacggcgcc gatctcgggc cgcatcggcc
     1741 gctcgctggt gaccgagggc gcgctggtca gcgccaccga gggcacgcag ctggccacca
     1801 tccagcagat caacccgctg tacctcaaca tcacgcagtc ggcggccgag gtgctgaagc
     1861 tgcgcgaagc gctgcaggcc ggcaagctgg cgcgcgccag cggcgccgaa gccgcccgcg
     1921 tgcaggtgct gctggaaaac ggccaggtct acggccagcc ggcgcgcctg ctgttcaccg
     1981 acctgagcgt cgaccccacc accggccagg tcaacctgcg cgctgaggtg cccaacccgg
     2041 gcggcctgct gatgcccggc atgtacgtgc gggcgcgcgt cgagcaggcc gaaatcgcca
     2101 atgcgatcct gctgccgcag caggccgtga cgcgcggcac gaaaggcgac agcgttatgg
     2161 tggtggcgcc cgatggcgcc gtggcgccgc gcccgatcaa gatcggcggc cagaaaggcc
     2221 aggactggat cgtcaccgag ggcctcaagc ccggcgagca ggtgatggtg gaaggcgcca
     2281 tgaagctgat gatgggcgcc aaggtggtca agcccgtgcc ctgggtgcca aaaacagcct
     2341 cggcgcaagc ccaacaagcg ccagcagcta caaaacctgc agcaagcgca ccggcagccg
     2401 ccgcgtcggc gccggccagc gcagcgtcga actaaaagaa gaggctgagc gcacatggca
     2461 cgctttttca ttgatcggcc catcttcgcg tgggtgatcg ccctgttcat catcgtgctg
     2521 ggcgccacgt ccatcacgcg gctgccggtg gcgcaatacc cgtcggtggc gccgcccacg
     2581 gtggtgcttt ccgccgccta ccccggcgct tcggcgcaga cgctggaaga ctcggtgctc
     2641 tcagtgatcg agcgcgaaat gaacggcgcg ccgggcctag cctacatgga gtcggtggcg
     2701 caggccaacg gcaccggcag catcaccctc tcgttcgagc cgggcaccaa cctcgatctg
     2761 gcgcaggtgg aggttcaaaa ccgcctggcc cgcgccacgc cgcggctgcc ctcggtggtc
     2821 aaccagcagg gcgtgcgggt cgagaagtcg caatccaact tcctcatgtt caccatgctg
     2881 tcctcgtccg agccaggcgc ggacgtgacg cggatcgccg actacgcggc gcgcaacgtg
     2941 cagcccgaac tgcagcgcct gcccggcatc ggccaggtcc agctgttcgg cgccgagcgc
     3001 gccatgcgca tctggctcga tccggccaag ctgcaaagct acggcctgac gccggccgac
     3061 gtcaacgccg ccatcgcggc gcagaacgct caggtgtcgg cgggcagcat tggcgacctg
     3121 ccgctggcgc ccgaccaggc caccaccgcc acggtgatcg tcccgggcca gctgtccacc
     3181 gtcgagcagt tcggcaacat cacgctgcgc gcgcagaccg gcggcgccgc cgtgcgcctg
     3241 aaggacgtgg cgcgcatcga actgggcggc cagagctacg ccaccgcagc gcgcctgaac
     3301 ggccagccca gcgtcggcat gggcattcag ctggcgcccg gcgccaacgc actggcggtc
     3361 gccaaggcgg tgaaaaagcg catggcagag ctggaggcct tcttcccggc cggtgtgagc
     3421 tacaccatcc cctacgacag ctccaccttc gtgcagatct cgatcgagca ggtggtgcac
     3481 accttgttcg aggcggtggc gctggtgttc atcgtcatgt tcatcttctt gcagaactgg
     3541 cgctacacgc tgatccccac catcgtggtg ccggtggcgc tgctgggcac gctggcggtg
     3601 atgctggtgg cgggcttctc gatcaacgtg ctgaccatgt tcggcatggt gctggtgatc
     3661 ggcatcgtgg tggacgacgc catcgtggtg gtcgagaacg tcgagcgcat catgagcgag
     3721 gaagggctct cgccggtgca ggccacgcgc aaggcgatgg ggcagatctc gggcgccatc
     3781 atcggcatca ccgtggtgct gatcgtggtg ttcgtgcccc tggccttctt ctcgggctcg
     3841 atcggcaaca tctaccgcca gttctcactg gtgatggcgg tgtcgatcgc gttctcggcc
     3901 ttcctggcgc tgtcgctcac gccggccttg tgcgccacgc tgctcaaacc ggtagaggcc
     3961 ggccaccacc acgagaaaaa aggtttcttt ggctggttca accgcggctt cacgcgcctg
     4021 acctcgcgct acgagggctg ggtcggcgcg ctgctcaagc gcggcggccg catgatgatc
     4081 gtctacctgg ccatcggcgc cgcggtgggc tggatgtaca cgcgcctgcc cacttcgttc
     4141 ctgccgcagg aagaccaagg ctacatcatc gtcaacattc aactgccggc gggcgccacg
     4201 ctggagcgca cgcaggcggt ggtcgagcag gtcgagggct acctgctcaa gcagcccgag
     4261 gtggccaaca tggtggcggt gatgggcttc tcgttctcgg gccaggggca gaacgcggcg
     4321 ctgtcgtttg tctcgctcaa accgtgggac gaacgcaagg gggccgagca cggcgccgac
     4381 gccgtggccg gacgcgccat gggcgcgctc tccggggtca aggacgcctt catcttctcg
     4441 ctgagcccac cgcccattcc cgaactgggc gtcagtgccg gcttcaacct gcgactgcag
     4501 gaccgcagca gccagggcca tgacgccttg ctggccgcgc gcaaccagtt gctgggcatg
     4561 accgcgcaaa gcaaggtggt ctccggcgta cggcccaacg gcctggaaga tgcgccgcaa
     4621 ttgcgcgtcg acatcaaccg cgaccaggcc gccgcgcagg gcgtcagctt ctcggccatc
     4681 aacagcgcca tctcgaccgc gctcggttcg tcctacgtca acgacttccc caacgccggc
     4741 cgcatgcagc gggtggtggt acaggccgac gccgctgcgc gcatgcagcc ggaggacatc
     4801 ctgcgcctga cggtgcaaaa cagcgccggg caggcggtgc cgctgtcggc ctttgccacc
     4861 accagctggg tcaccggccc gatgcagacc gtgcgctaca acggctaccc ggccatgcgc
     4921 atcgaaggtg ccgcggcgcc gggccagagt accggccagg cgatggccga gatggaggcg
     4981 ctggcggcca agctgccgcc cggcttcggc atcgaatgga ccggccagtc gcgcgaggaa
     5041 aagctggccg gcaaccaggc gatcctgctt tacgccttcg ccatcctggc ggtgttcctg
     5101 gcgttgtcgg cgctgtacga gagctggtcg atcccgctgg cggtgatcct ggtggtgccg
     5161 ctgggcgtgc tgggcgtgct gatcggcgtg aacatgcgcg ggctgaccaa cgacgtgtac
     5221 ttccagatcg gcctgatcac catcatcggc ctgtcggcga agaacgccat cctgatcatc
     5281 gagttcgcca aggacttgca ggcccagggc cgcagcgtgc tgcaggcggc gctggaagcg
     5341 gcgcacctgc gcttccgccc catcctgatg acctcgctcg ccttcaccgc cggcgtggtg
     5401 ccgctggcca ttgcctcggg cgccagctcg gccagccagc gcgccatcgg caccggcgtg
     5461 atcggcggca tggtggtggg caccgttctt tccgtgttcc tggtgccggt gttcttcctg
     5521 gtcgtgcgct ccatcttcaa gggtggcaag cgcgagcagg aaatggccca gcagcacgca
     5581 cgcagcgccg gtgtcggcag cggcgaaccc gtggagccca caccgtgatc cccttcaatt
     5641 cccccttgcc cgcgatgaaa gcacgcgccc tcgttcgctg gctggcgacg cccttgtgcg
     5701 tggccacgct cgccgcctgc tcgttcgtgc ccaagtacga acaacccgcc gcccccgtca
     5761 gcgccacctg gcccgattcg gccggcgcgc aggtggaagg cggcgttgcc gccgccgacc
     5821 tgccgtggca gcagttcgtg cacgacgcgc agctgcgcga agtgatccag ctcgcactgg
     5881 agaacaaccg cgacctgcgc gtggcggtgc agaacatcga gcaggcgcgc gcgc
//
LOCUS       DAOMVO010000142         5903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3636_length_5903_cov_13.674419, whole genome shotgun sequence.
ACCESSION   DAOMVO010000142 DAOMVO010000000
VERSION     DAOMVO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3636_length_5903_cov_13.674419"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKV30_07970"
     CDS             complement(<1..859)
                     /locus_tag="PKV30_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30184.1"
                     /translation="MKLRQLAHLTALAEQGSFSRAADCLHLTQSALSRSLQTLEDELG
                     ARLIDRAGKRSTLTPLGEAVLARARRITLEAAEIQRSAELLRQGEAGNIRVGLGSGPG
                     ALLMTPLLTHVAAHYPQVRVAIARGSTELQVAQLRQRELDALVVDARRISYASDLVIE
                     PLTELRGGFVSRAGHPLAQLASVSFAQLMAYPVATTPLSDEVARMLVAHYGPGADPAR
                     MAALVCEDVGSLLDAAAETDAVFLGVIAAARSGIEAGRLAELRMAPPLAGGARFAYVR
                     LAGRTEAPVM"
     gene            973..1965
                     /locus_tag="PKV30_07975"
     CDS             973..1965
                     /locus_tag="PKV30_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL30185.1"
                     /translation="MPFVRRHLIQFAAASLATLALGAAAQSYPTKPVTLMVPYSAGGL
                     SDNIARRVNVPLAATLGQPAIIENLGGASGSIAAQKVVNAPSDGYMVFQGSPNELILA
                     PLAIPSIKYKPEDFRQVHRVALAPMAVFARANLPVKNADELAAYAAKVAKEGKPLTYA
                     SVGVGSFYHLLGGKMSKDLGIPMTHVPYKGGADAFRDLVGDQVDIFITPYSLAQVEFV
                     KQGKVKAVAALTPQRQRLLPDTPTTAESKALKNFQYEIGTGYFVKRDTPEPVVQKLHQ
                     ALQKTLTDTQFKAGLFAMGQEVSPSQTLSEADKAYKDETAAYQSIAKAIGLNPQ"
     gene            1974..3194
                     /locus_tag="PKV30_07980"
     CDS             1974..3194
                     /locus_tag="PKV30_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRL30186.1"
                     /translation="MNAALPVAHHATLDALQASADDFVAVRRDIHQHPELGYEEFRTS
                     ELVAERLASWGYTVTRGLGGTGVVGQLRRGDGARRLGLRADMDALPIHEATGLPWAST
                     HAGRMHACGHDGHTAMLLSAARHIAQHGRFSGTLNLIFQPAEEGLGGARQMMDDGLFE
                     RFPCDAIFGMHNMPGHRQGQLLLRDGATMASSDGLTITLTGKGSHGAMPHHGIDPVVA
                     GASIVLGLQSIVARNVDPQAMAVITVGAFQAGQAHNVIPNEAVLKLSVRSLDRDVRAT
                     LLRRIEELIDAQARSFNLTWTMECVTGYPVLVNTAAETDFARQVAVELVGADNVVPQA
                     APLSGSEDFAFMLEQVPGSYLLIGNGDNSGEPHNGGHGARMVHNPGYDFNDANLPIGA
                     AYWALLTERFLTED"
     gene            complement(3234..4121)
                     /locus_tag="PKV30_07985"
     CDS             complement(3234..4121)
                     /locus_tag="PKV30_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL30187.1"
                     /translation="MARPNDSSRRDQPDTQWLERMYNNRMRVPDHGDYFARWAAESAL
                     VRRSLGGEVEVPYGDAARERLDAFAAPRVGRGARAPLLVFIHGGYWRSLDKSYHSFVA
                     GAAHALGAATVVPNYSLCPQARISDITLQMAHAVAWAWRHARSLNADPRRISVVGHSA
                     GGQLAAMMLACAWDVLDPSLPRQVVRRALSVSGLYDLEPLMHTPSLQEVLRLDPNQVA
                     ACSPARLPAPARGELWALVGGDESGEYLRQQRLIRQAWGRERVPLAQALPGLNHFSVV
                     DALVDPKQRAHRLLRQLIA"
     gene            4344..4416
                     /locus_tag="PKV30_07990"
     tRNA            4344..4416
                     /locus_tag="PKV30_07990"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4377..4379,aa:Ala,seq:cgc)
     gene            complement(4504..5337)
                     /locus_tag="PKV30_07995"
     CDS             complement(4504..5337)
                     /locus_tag="PKV30_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRL30188.1"
                     /translation="MTVWIDTHCHLDAPEFSADVAAVRARAAAKNVAICVVPAVEASA
                     FEGVRALAHAGGDAYALGIHPLYVERAHDEDLLRLDEALTRHAGDPRLVAVGEIGLDY
                     FVPELAAPGPLRKRQEHFYREQLKLARRHGLPVIVHVRRSVDQVLKRLRDIGVAGGIA
                     HAFNGSRQQADAFLGMGFKLGFGGAVTYDRSTRLRALAAELPPEAIVMETDAPDIPPH
                     WLYATAEQRAAGAPQGRNQPAELPRIAGEVARLRGVSSGEWAATTTANALAALPRLAR
                     L"
     gene            5489..>5903
                     /locus_tag="PKV30_08000"
     CDS             5489..>5903
                     /locus_tag="PKV30_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HRL30189.1"
                     /translation="MAPAGDAPADPMADALPGPQETAAPSPTRRQRLRAALAHPGLMW
                     VAVALLGGALAFTAGPWGRTAPRKLTQDDINAAVMHTLTNKVLPSQAARAAATVGPAL
                     VRVEGLVAGKKDRKGHAEMGRGVGTGVVIIDNGTIL"
BASE COUNT          974 a         2103 c         1976 g          850 t
ORIGIN      
        1 gcatcaccgg cgcctcggtg cggccggcca ggcgcacgta ggcaaagcgc gcgccccccg
       61 ccagcggcgg cgccatgcgc agctccgcca gccggccggc ctcgatgccg ctgcgtgcgg
      121 cggcgatcac ccccaggaac acggcgtcgg tttcggccgc cgcgtccagc aggctgccga
      181 cgtcctcgca caccagcgcc gccatgcgtg ccgggtcggc accagggccg tagtgcgcca
      241 ccagcatgcg cgccacctcg tccgacagcg gcgtggtggc caccgggtag gccatcagct
      301 gcgcgaagct gaccgaggcc aattgcgcca gcgggtggcc ggcgcggctg acgaagccgc
      361 cgcgcagctc ggtcagcggc tcgatcacca ggtcgctggc gtagctgatg cggcgcgcgt
      421 ccaccaccag cgcgtccagc tcgcgctggc gcagctgtgc cacctgcagc tcggtcgagc
      481 cgcgcgcgat ggccacgcga acctgagggt agtgtgccgc cacgtgggtc agcagcggcg
      541 tcatcagcaa cgcgcccggg cccgagccca gcccgacgcg gatgttgccg gcctcgccct
      601 ggcgcagcag ctcggcgctg cgctggatct cggccgcctc cagcgtgatg cggcgcgcgc
      661 gcgccagcac cgcctcgccg agcggcgtca gagtgctgcg cttgccggcg cggtcgatca
      721 ggcgcgcgcc cagttcgtcc tccagcgtct ggaggctgcg gctgagcgcc gactgcgtca
      781 ggtgcaggca atcggcggcg cgcgagaacg agccttgttc agccagtgcg gtcaggtggg
      841 caagttggcg cagcttcata tttatgacat taaatcattc atacgttgat ttcaattcat
      901 ttgacacatg atctgacgtg caacatattg ccggtctgcc gcattccatt ccaacgatcc
      961 cggagacaag ccatgccatt cgtccgccgc catctcatcc agttcgccgc cgcctcgctg
     1021 gccaccctcg ccctgggcgc cgccgcgcaa agctacccga ccaagcccgt cacgctgatg
     1081 gtcccctatt ccgcgggcgg cctgtcggac aacattgcgc gccgcgtcaa cgtgccgctg
     1141 gcggcgacgc tcggccagcc cgccatcatc gaaaacctgg gcggcgccag cggctcgatc
     1201 gcggcgcaga aagtcgtcaa cgcgccgtcc gacggctaca tggtgtttca gggctcgccc
     1261 aacgaactga tcctggcgcc gctggcgatc ccgtcgatca agtacaagcc ggaagacttc
     1321 cgccaggtgc accgcgtcgc gctggcgccg atggccgtgt tcgcgcgtgc caacctgccc
     1381 gtgaagaacg ccgacgagct ggccgcttat gccgccaagg tcgccaagga aggcaagccg
     1441 ctgacctacg ccagcgtcgg cgtcggctct ttctaccacc tgctgggcgg caagatgagc
     1501 aaggaccttg gcattccgat gacgcacgtg ccgtacaagg gcggagccga tgccttccgc
     1561 gacctggtgg gtgaccaggt ggacatcttc atcacgccct acagcctggc gcaggtcgaa
     1621 ttcgtcaagc aaggcaaagt caaggccgtg gcggcgctga cgccgcagcg ccagcgcctg
     1681 ctacccgaca cgccgaccac cgccgagagc aaggcgctga agaacttcca gtacgagatc
     1741 ggcaccggct acttcgtcaa gcgcgacacg ccggaaccgg tagtgcagaa gctgcatcag
     1801 gcgctgcaga agacgctgac cgacacccag ttcaaggccg gcctgttcgc catgggccag
     1861 gaagtctcgc cgtcccagac gctgtccgaa gccgacaagg cctacaagga cgagaccgcc
     1921 gcctaccagt cgatcgccaa ggccatcggc ctgaacccgc agtgagcccg gccatgaacg
     1981 cagccctgcc cgtcgcccac cacgccacgc tggacgcgct gcaggccagc gcggacgatt
     2041 tcgtcgccgt gcggcgggac atccaccagc acccggaact cgggtacgaa gagttccgca
     2101 ccagtgagct ggtggccgaa cgcctggcca gctggggcta caccgtcacg cgcggcctgg
     2161 gcggcacggg cgtggtcggc cagctgcgac gcggcgacgg cgccaggcgt ctgggcctgc
     2221 gcgccgacat ggacgcgctg ccgatccacg aggccaccgg cctgccctgg gccagcaccc
     2281 atgcgggacg catgcacgcc tgcggccacg acggccacac cgccatgctg ctgtcagcgg
     2341 cgcggcacat cgcgcagcac gggcgctttt cgggcacgct gaacctgatc ttccagccgg
     2401 ccgaggaagg tctgggcggc gcccgccaga tgatggacga cggtctgttc gagcgctttc
     2461 cgtgcgacgc catctttggc atgcacaaca tgcccggtca ccgccagggc cagctgctgc
     2521 tgcgcgacgg cgccaccatg gcctcgtccg acggactcac catcacgctg accggcaagg
     2581 gcagccatgg cgccatgccg caccacggta tcgacccggt ggtggcgggc gcctccatcg
     2641 tgctgggatt gcagagcatc gtcgcgcgca acgtcgaccc gcaggcgatg gcggtcatca
     2701 cggtgggcgc gttccaggcc ggccaggctc acaacgtgat ccccaacgag gcagtgctga
     2761 agctcagcgt gcgctcgctc gaccgtgacg tgcgcgccac cctgctgcgc cgcatcgagg
     2821 agctgatcga cgcccaggcc agatccttca acctgacgtg gaccatggaa tgcgtgaccg
     2881 gttacccggt gctggtcaac acggcggccg aaaccgattt cgcgcgccag gtggccgtgg
     2941 agctggtcgg cgccgacaac gtggtgccgc aggccgcgcc gctgtcgggc agcgaagact
     3001 ttgccttcat gctggaacaa gtgcccggca gctacctgct gatcggcaac ggcgacaact
     3061 ccggcgagcc gcacaacggc ggccacggcg cccgcatggt gcacaacccg ggctatgatt
     3121 tcaacgatgc caatcttccg atcggcgccg cctactgggc actgctgacc gagcgcttcc
     3181 tgaccgaaga ttgatcgctt agggcgccgg cgccggtccg cccggcggcc acgtcaggcg
     3241 atgagctgac gcagcaaccg gtgcgcgcgc tgcttcgggt cgaccagcgc gtcgacgacg
     3301 ctgaaatggt tcagccccgg cagagcctgg gccaggggca cgcgctcgcg cccccaggcc
     3361 tggcgaatca ggcgctgctg gcgcaggtac tcgccgcttt cgtcgccgcc gaccagcgcc
     3421 cacagctcgc cacgtgccgg cgccggcagc cgcgccgggc tgcacgccgc cacctggttg
     3481 ggatccagcc gcagcacctc ctgcaagctg ggcgtgtgca tcagcggctc caggtcgtac
     3541 agacccgaca cgctgagcgc gcgccggacg acctgccgtg gcagcgaggg atccagcacg
     3601 tcccaggcgc aggccagcat catcgccgcc agctggccgc cagccgaatg accgaccacg
     3661 ctgatgcgcc gcggatcggc gttgagcgag cgcgcgtggc gccaggccca ggccacggcg
     3721 tgcgccatct gcagcgtgat gtcactgatg cgcgcctgcg ggcacaggct gtagttgggc
     3781 accaccgtgg ccgcacccag cgcatgcgcg gcgccggcaa cgaagctgtg atacgacttg
     3841 tcaagcgaac gccagtagcc gccgtgaatg aacaccagca gcggcgctcg ggcgccccta
     3901 cccacgcgcg gtgcagcgaa ggcgtccagc cgctcacgcg cggcatcgcc ataggggacc
     3961 tccacctcgc cccccaacga ccgccgcacc agcgccgatt cggccgccca gcgcgcgaag
     4021 tagtcgccat ggtcagggac gcgcatgcgg ttgttgtaca tgcgctccag ccactgggtg
     4081 tcgggctgat cgcggcggga agaatcgttg gggcgggcca tgtgaagcga tattgtcgca
     4141 aacagcgcag cggtcggcgc ccggcggccg gccagcgctt tcaatcggtg agggttggaa
     4201 cctgcacgaa taaggccgtt ggcccactac agacgggcgc caccagctat cgttttggaa
     4261 gccaccacaa gaaaaagcgc gtcatgccgc agccggcgcg actcgactat gctggcaaag
     4321 cgttaaacta ctcatggctt tcgggggggg tagctcagct gggagagcgc cgcgttcgca
     4381 atgcggaggt cgggagttcg atcctcctcc tctccacaaa aagtccttat ttcatgcggc
     4441 ctgcgggcct tttttgttgg ggcaaccacg agctgcctca ctgtgaaggg tacttgtcgc
     4501 gattcaaagt cgcgcaagac gcggcagcgc cgccaacgca ttcgccgtgg tcgtggctgc
     4561 ccactcgccg cttgaaacgc cccgcaaccg cgccacttcg cccgcgatgc gcggcagctc
     4621 ggctggctgg ttgcgcccct gaggcgcccc cgctgcacgc tgctcagcgg tggcgtagag
     4681 ccagtgcggc gggatgtcgg gggcatcggt ttccatcacg atggcttcgg gcggcaactc
     4741 ggccgccagc gcgcgcaggc gcgtggagcg gtcgtaggtg acggcgccgc cgaaaccgag
     4801 cttgaaaccc atgcccagaa aagcatcagc ctgctgccgg ctgccgttga acgcatgagc
     4861 gatgccgccc gccacgccaa tgtcgcgcaa gcgtttcagc acctgatcga ccgagcgccg
     4921 cacgtgcacg atcacgggca gaccatgtcg gcgcgccagc ttgagttgct cgcggtagaa
     4981 atgctcttgc cgcttgcgca gcggacccgg ggcggcgagt tcgggcacga agtagtcgag
     5041 gccgatttcg cccactgcca ccaggcgcgg gtcgcccgca tggcgcgtca gcgcctcgtc
     5101 caggcgcagc aggtcctcat cgtgcgcgcg ctcgacgtac agcgggtgaa tgccgagcgc
     5161 gtaggcgtcg ccgccggcgt gcgccaacgc ccgcacacct tcgaaggcgc tcgcttcgac
     5221 ggcgggaacg acgcagatcg ctacgttttt tgcagctgcc cgcgcccgca cggcggcgac
     5281 atcggccgaa aactcggggg catccagatg gcagtgggtg tcgatccaga ccgtcatgcg
     5341 cgcatcatgc cccagggcgc tggtgacgtt gacgcggctt gcagtggcgc ggcgggcgcc
     5401 cgtcgtgata ccgtgtgatc tctggtcctg aagtcgaggg tgaaccatgc gtcgaaccgc
     5461 gctttacagt cgatcccgcc cgccctgcat ggcaccggca ggcgacgccc cggccgaccc
     5521 gatggcggac gctttgcccg gcccccaaga gacggccgcg ccgtcgccca cgcgccggca
     5581 acggctgcgc gccgcgctgg cgcacccggg cctgatgtgg gtggcggtcg cgctgctggg
     5641 cggcgcgctg gcctttactg ccggcccctg gggccgcacg gcgccgcgca agctgacgca
     5701 ggacgacatc aacgccgccg tcatgcacac gctgaccaac aaggtgctgc cctcgcaggc
     5761 ggcgcgcgcg gccgccactg tgggcccggc gctggtacgc gtcgagggtc tggtggccgg
     5821 caagaaagac cgcaagggcc acgccgaaat gggccgcggc gtgggcacgg gcgtggtgat
     5881 catcgacaac ggcaccatcc tga
//
LOCUS       DAOMVO010000143         5883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3660_length_5883_cov_13.531572, whole genome shotgun sequence.
ACCESSION   DAOMVO010000143 DAOMVO010000000
VERSION     DAOMVO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_5883_cov_13.531572"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKV30_08005"
     CDS             <1..185
                     /locus_tag="PKV30_08005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HRL30190.1"
                     /translation="IQFDAAANPGNSGGPLVTMDGQVVGIVTAILNPTPARTFLGIGF
                     AVPIENAAAAAGQPPF"
     gene            214..1233
                     /locus_tag="PKV30_08010"
     CDS             214..1233
                     /locus_tag="PKV30_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL30191.1"
                     /translation="MTDADIRRFEPNPATAELMEQILYEVKRVVVGQDRFLERVMVAL
                     LAGGHLLVEGVPGLAKTLTVKTLAGVLQGQFKRIQFTPDLVPADLIGTRIYNQRDGDF
                     STALGPVFTNLLLADEINRAPAKVQSALLEVMQERQVTIAGQTHFVPEPFLVMATQNP
                     IETEGTYPLPEAQVDRFMMKVLVGYPTDEEEFVIAQRVTGPRIDVNPVASMEQLAALQ
                     RECRQVYVDPSLVQYAVKLVTATRAPAEVGLKDLAPMITFGASPRATIGLIEGAKALA
                     MMRGRTYALPEDMSDLVPDVLRHRLVLSYEALSEGLAADPVVLRIMAKVPPPPKPLAH
                     DRLAA"
     gene            1432..2469
                     /locus_tag="PKV30_08015"
     CDS             1432..2469
                     /locus_tag="PKV30_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRL30192.1"
                     /translation="MIRWLRREKTPPPHAVPNPPRAETATPNAENILRRLEWSVLRRL
                     DGLLLGDYRTLMRGAGLDLADLREYQMHDDVRHIDWNVTARMQTPHVRVFTEDREMAA
                     WFLLDLSPSVDFGSGPQRKRQVSAEFVAVLARLLTRHGNRVGALLYAAGVEKMIPARA
                     SRQHVLHLLHAMQSVQPAATTKQEAARARQSSAGGTKLSELLLAAANTIRRRSTIFVV
                     SDFISEPGWEKPLGQLATRHEVVAVRLTDPLELALPDLGLLTLRDAETGEQLLVDTHS
                     PAFRRRFAAIAAQREAELRGSLARAGVDTLELSTDDDLASAIVRFTDLRRQRARVAAG
                     GGMPAHLRKTA"
     gene            2491..3585
                     /locus_tag="PKV30_08020"
     CDS             2491..3585
                     /locus_tag="PKV30_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRL30193.1"
                     /translation="MNFTWPAFLKWPAGVPQPNFLWPQFLWLLLLLPLLVLLYWWLMR
                     RKNKLAVRYASLAIVKEAMGRGPGWRRHVPPLLMLLALAAMLLASARPVAVVALPSNQ
                     QTIILAMDVSGSMRATDVEPNRLVAAQNAAKAFLKDLPRDVRVGIVAFAGSAQVAQLP
                     TVNRDDLVTAIDRFQLQRATATGNAIVISLATIFPNAGIELQQLSFDRMRGGMGRSLD
                     QPPPKQNEFIPVPPGSYNSAAIIMLTDGQRTTGVDPIEAAKLAADRGVKVYTVGVGTV
                     DGETIGFEGWSMRVRLDEETLKAIAQKTAAEYYYAGTAQDLQKVYQNLSSRLTLEKKE
                     TEVTSLFALLGAVLVLLAGGLSMWWFNRLL"
     gene            3685..4668
                     /locus_tag="PKV30_08025"
     CDS             3685..4668
                     /locus_tag="PKV30_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRL30194.1"
                     /translation="MTTQPASAPPDWLYLVAQATQAPSGHNSQPWLFKLNPDSIEVHP
                     DLRRSLPAVDPEHRELYVSLGCAVENLCLAATEKGYASQVTVADSGVITVALTHNPGI
                     SPDPLAAQIGLRQSNRRVYSGCLLSEHTIAQLAAAAQGEGVGFHLYPRDTPAFEAIAE
                     FVYRGNTVQMHDAAFKTELKSWMRFNRKHQDSTLDGLSYAVFGAPNLPAFLSRPIMAM
                     QINAATQNKNDSKRIQSASHLALFTTASDSLREWVALGRSLQHFWLTATALGVACAHL
                     NQPNEVPRLAQEMAASLQLGAERPTVLLRLGYADKLPYSKRRPVSEVLMPS"
     gene            complement(4731..5186)
                     /locus_tag="PKV30_08030"
     CDS             complement(4731..5186)
                     /locus_tag="PKV30_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30195.1"
                     /translation="MVNLLVLKAGDDTGPGVAVVAAESGRINVFTSALHRPVPLAWLS
                     ALLLAGCASAPTPVPEAKQTAELPTSLAQRIATSVRDRPQAIWSLRYQGQPAFYVQAP
                     CCDQFNTLHDAAGATICVPDGGFIGRGDGKCSAPLPPSDQMRLVWERAR"
     gene            5185..5637
                     /gene="yiaA"
                     /locus_tag="PKV30_08035"
     CDS             5185..5637
                     /gene="yiaA"
                     /locus_tag="PKV30_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HRL30196.1"
                     /translation="MSMQPGRPSGAFVAASWAALAIGAIVYLIGLWNAQMQLNEKGYY
                     LTLLAYGLFSAISLQKSVRDRAEGIPVTGMYYNLCWFSVVLVLLLLTIGLWNAKLADS
                     EKGFYAMAFLLALFGAVAVQKNVRDLAATGGSAPMLRRRERPDTPPSA"
     gene            complement(5665..>5883)
                     /locus_tag="PKV30_08040"
     CDS             complement(5665..>5883)
                     /locus_tag="PKV30_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HRL30197.1"
                     /translation="RALVTQPACVLADEPTGNLDRGTADGVFMLMLELAANHGTAFVL
                     VTHDDAMAARCGRALRLVQGRLAAPEAR"
BASE COUNT         1015 a         2041 c         1866 g          961 t
ORIGIN      
        1 tgatccagtt cgacgccgcc gccaaccccg gcaactcggg cgggccgctg gtgaccatgg
       61 acgggcaggt ggtgggcatc gtcaccgcca tcctcaaccc gacgccggcg cgcacgtttt
      121 tgggcatcgg ctttgccgtg ccgattgaaa acgccgccgc ggccgctggc cagccgccgt
      181 tctgatcact cttattttcg gagcgccctg cgcatgactg acgccgacat ccgccgtttt
      241 gaacctaatc ccgccaccgc cgagctgatg gagcaaatcc tctacgaggt caagcgcgtg
      301 gtggtcgggc aagaccgctt tttggagcgc gtgatggtcg cgctactcgc cggcggccac
      361 ctgctggtcg agggcgtgcc gggcctggcc aagacgctga cggtgaagac gctggccggt
      421 gtgctgcaag gccagttcaa gcgcatccag ttcacgcccg acctggtgcc ggccgatttg
      481 atcggcacgc gcatctacaa ccagcgcgac ggcgatttca gcactgcgct gggccccgta
      541 ttcaccaacc tgctgctggc cgacgagatc aaccgcgcgc ccgccaaggt gcaaagcgcg
      601 ctgctggaag tcatgcagga gcgccaggtc accatcgccg gccagacgca tttcgtgccc
      661 gagccgttcc tcgtcatggc cacgcagaac cccatcgaga ccgaaggcac ctatccgctg
      721 cccgaggcgc aggtcgaccg cttcatgatg aaggtgctgg tcggctaccc gaccgacgag
      781 gaggagttcg tcatcgccca gcgcgtgacc ggcccgcgca tcgacgtcaa ccccgtggcc
      841 agcatggaac agctggctgc gctgcaacgt gaatgccggc aggtgtacgt cgacccctca
      901 ctcgtgcagt acgccgtcaa gctggtgacc gccacgcgcg cgccagccga ggtcgggctg
      961 aaggatctgg cgcccatgat cacctttggc gccagcccgc gcgccaccat cggcctgatc
     1021 gaaggcgcca aggcgctggc catgatgcgc gggcgcacct acgccctgcc cgaggacatg
     1081 agcgacctgg tgcccgacgt gctgcgacat cgcctggtgc tgtcgtacga ggcgctgtcc
     1141 gaagggctgg ccgccgaccc ggttgtgctg cgcatcatgg ccaaggtgcc gccgccgccc
     1201 aagccgctgg cgcacgacag gctggcggcg tgacggcggc gccacggtga tgtcactggc
     1261 gacccaactc ccccgccatt ccggcgcaag ccggaatcca gacggcgtgt ccacaaccaa
     1321 gcctcgcttg gattccggct tgcgccggaa tgacggggat gggatgggat gcgccgcaca
     1381 tccatctatc gaagaatctc ctgccgcgca tcgtcgccgc agcaccgccc catgatccgc
     1441 tggttgcgcc gcgaaaaaac cccgccgccc cacgcggtgc ccaacccgcc gcgcgctgaa
     1501 accgccacgc ccaacgccga gaacatcctg cgccgcctcg aatggtcggt gctgcgccgg
     1561 ctggatggcc tgctgctggg cgactaccgc acgctgatgc gcggtgcggg gctcgatctg
     1621 gccgatctgc gcgaatacca gatgcacgac gacgtgcgcc acatcgactg gaacgtgacg
     1681 gcgcgcatgc agaccccgca cgtgcgcgtg ttcaccgaag accgcgagat ggcggcgtgg
     1741 tttctgctgg acttgagccc ctcggtcgat ttcggctccg gcccacaacg caaacgccag
     1801 gtgtcggctg agtttgttgc cgtgctggcg cggctgctca cgcggcacgg caaccgcgtc
     1861 ggcgcgctgc tgtatgccgc gggggtcgag aagatgattc ccgcacgcgc cagccgccag
     1921 cacgtgctgc atctgctgca cgccatgcag tcggtgcagc cggcggccac tacaaaacaa
     1981 gaagctgctc gcgcgcgcca gtcaagcgct ggcggcacaa aactgtctga attgttgctg
     2041 gcggcggcca acaccatccg ccgccgctcg accatcttcg tggtgtcgga tttcatcagc
     2101 gaaccggggt gggaaaagcc gctcggccag ctcgccacgc ggcacgaagt ggtggccgtg
     2161 cgcctgaccg acccgctgga gctggcgctg cccgacctcg gcctgctgac cctgcgcgat
     2221 gccgaaaccg gcgagcaact gctggtcgac acccacagcc cggcgtttcg ccgtcgcttt
     2281 gccgccattg ccgcccagcg cgaagccgag ttgcgcggtt cactggcgcg cgccggcgtc
     2341 gacacgctgg agctgtcgac cgacgacgat ctggccagcg ccatcgtgcg ctttaccgac
     2401 ctgcgccgcc agcgggcccg cgtggccgcc ggcggcggaa tgcccgcgca cctgcgcaaa
     2461 accgcctgat cgcgcgacaa tggggccgcc atgaatttca cctggcctgc cttcctcaag
     2521 tggcctgcgg gcgtgccgca gccgaatttt ctgtggccgc agttcttgtg gctgctgctg
     2581 ctgttgccgc tgctggtgct gttgtactgg tggctgatgc ggcgcaagaa taagctcgcc
     2641 gtgcgctacg cgagcctggc gatcgtgaaa gaggccatgg gccgcggccc cggctggcgc
     2701 cgccatgtgc caccgctgtt gatgctgctg gcgctggcgg ccatgctgct ggccagcgcg
     2761 cggccggtgg cggtggtcgc actgccctcc aaccagcaga ccatcatttt ggccatggac
     2821 gtctcgggca gcatgcgcgc caccgacgtc gagccgaacc gtctggtggc ggcgcaaaac
     2881 gcggccaagg cgttcctgaa agacttgccg cgcgacgtca gggtcggcat cgtcgccttt
     2941 gccggctcgg cgcaggtggc gcagttgccc accgtcaacc gcgacgacct ggtcaccgcc
     3001 attgaccgct ttcagctgca gcgcgccacg gccacgggca acgccatcgt gatctcgctg
     3061 gcgacgatct ttcccaatgc cggcatcgag ctgcagcagc tcagctttga ccgcatgcgc
     3121 ggcggcatgg gccgctcgct agaccagccg ccgcccaagc agaacgaatt catccccgtg
     3181 ccgccgggct cgtacaactc ggccgccatc atcatgctga ccgacggcca gcgcaccacc
     3241 ggcgtcgacc cgatcgaagc cgccaagctg gctgccgacc ggggcgtcaa ggtctacacc
     3301 gtcggcgtgg gcacggtgga tggcgaaacc atcggcttcg aaggctggtc gatgcgcgtg
     3361 cggctggacg aagaaacgct gaaagccatc gcgcagaaaa cggccgccga gtactactac
     3421 gccggcaccg cacaagactt gcagaaggtc taccagaacc tctcaagccg cctgacgctg
     3481 gagaaaaagg aaaccgaagt cacatcgctt tttgcactgc tgggcgccgt gctggttctg
     3541 ctggctggtg ggctttccat gtggtggttc aaccgtttgc tgtaagccgg ccggctacct
     3601 ggcgaagtgc gacccgggcg tttagttagc ggctacacgc cttgacgccc gacgaaagat
     3661 gaacggagtt tttttgcatt catcatgaca acccaacccg caagcgcccc acccgactgg
     3721 ctctacctag tggcacaggc cacgcaagcc ccctcaggcc acaacagcca accgtggctc
     3781 ttcaagctca atccggacag catcgaggtt caccccgacc tgaggcgatc attgcctgcg
     3841 gtcgatcccg agcaccggga gctgtatgtc tcgctaggct gtgcggttga aaacctctgc
     3901 cttgccgcca cagagaaagg ctatgccagc caggtgacgg tggccgattc cggcgtcatc
     3961 accgtggcgc tgacccacaa cccaggcatc tcgcccgacc cgttggcagc ccagattggc
     4021 ttgcggcaaa gcaaccgccg ggtctatagc ggctgcttgc tctcagagca caccatcgct
     4081 cagttggcgg ccgctgcaca gggggaaggc gtcggctttc acctctaccc gcgtgacacc
     4141 ccggcgtttg aagcaatcgc ggaattcgtc taccggggca acaccgtgca gatgcacgac
     4201 gcagctttca aaaccgagct gaaaagctgg atgcgcttca accgaaagca ccaggacagc
     4261 acgctggacg gcttgagcta cgccgttttc ggcgcaccga acttgcccgc cttcctatct
     4321 cggccgatca tggccatgca gatcaacgcg gccacgcaga acaagaacga cagcaagcgg
     4381 atccaatccg cgtcgcacct cgcgctgttc accaccgctt cggattcgct gcgcgaatgg
     4441 gttgccctgg ggcgatcgct gcagcacttc tggctcaccg ccaccgcgct cggcgtggcg
     4501 tgcgcgcacc ttaaccagcc caacgaagtg cctcgccttg cgcaggagat ggccgcaagc
     4561 ctacagctgg gcgcggaacg cccgacagtg ctgcttcgcc tgggctacgc agacaagttg
     4621 ccgtactcca agcgccggcc agtcagcgaa gtgctaatgc caagctgaaa aaccggcggc
     4681 gcctgccacg cggccgcagt gccttcagcc agcttgccaa ggcggcccac tcagcgggcg
     4741 cgctcccaca ccagccgcat ctggtcagag ggcggcaacg gtgccgaaca cttgccgtcg
     4801 ccgcgtccga tgaagccgcc gtcgggcacg cagatcgtgg cgccggccgc gtcgtgcaag
     4861 gtgttgaact ggtcacagca cggcgcctgc acgtagaacg ccggttgacc ttgatagcgg
     4921 agtgaccaga tcgcctgcgg gcgatcccgc accgatgtcg cgatacgttg cgccaaggat
     4981 gtcggcaatt cagccgtctg tttcgcctcc ggcacgggcg tgggcgcgct ggcgcagcct
     5041 gccagcaaca aggcgctcag ccaagccaac ggaacaggac gatgcagcgc ggaggtgaag
     5101 acgttgatgc gcccggattc tgccgcgacg acggccacac cgggccccgt atcatcacca
     5161 gctttcaaaa cgaggaggtt caccatgtcc atgcaacccg ggcggccgtc tggcgccttt
     5221 gtggccgcgt cctgggccgc gttggccatc ggcgccatcg tttacctgat cgggctttgg
     5281 aacgcccaga tgcagctcaa cgaaaaaggc tactacctca ccctgctggc ctacggcctg
     5341 ttctcggcca tctcgctgca gaaatcggtg cgcgaccgcg ccgaggggat accggtgacc
     5401 ggcatgtact acaacctgtg ctggttctcg gtcgtcctgg tgctgttgct gctgaccatc
     5461 ggcctgtgga acgccaagct ggccgacagc gaaaaaggct tttacgcgat ggcgtttttg
     5521 ctggcgctgt tcggcgccgt ggcggtgcaa aagaacgtgc gcgatctggc cgccaccggc
     5581 ggcagcgcgc ccatgctgcg ccgccgagag cggccggata cgccgccgtc ggcctgaagc
     5641 tctgcggcgc gctgatcgtc gctgtcagcg cgcctcaggc gccgccagcc gcccctggac
     5701 cagcctgagc gccctgccgc agcgcgccgc catcgcgtcg tcgtgcgtga ccagcacgaa
     5761 ggcggtgccg tggttggcgg ccagttccag catcagcatg aacacgccat cggccgtgcc
     5821 gcgatccagg ttgccggtcg gctcgtcggc cagcacgcaa gcgggttgcg tgaccagcgc
     5881 gcg
//
LOCUS       DAOMVO010000144         5837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3707_length_5837_cov_20.533207, whole genome shotgun sequence.
ACCESSION   DAOMVO010000144 DAOMVO010000000
VERSION     DAOMVO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3707_length_5837_cov_20.533207"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKV30_08045"
     CDS             complement(<1..309)
                     /locus_tag="PKV30_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL30198.1"
                     /translation="MTDAPTPDSQTPANAGGYGEGAIQILEGLEAVRKRPGMYIGDTS
                     DGTGLHHLVFEVVDNSIDEALAGYCDDIVVTIHSDNSISVVDNGRGIPTGVKMDDKHE
                     P"
     gene            complement(409..1542)
                     /gene="dnaN"
                     /locus_tag="PKV30_08050"
     CDS             complement(409..1542)
                     /gene="dnaN"
                     /locus_tag="PKV30_08050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRL30199.1"
                     /translation="MIVLKTTQNQLLSALQSVAGIVERRHTLPVLANVLLRKSGETVQ
                     LTTSDLEIQIRTAAQLGGDSGNFSTTVGARKLIDILRTMPADQTVSLESQASKLILKG
                     GKSKFTLQTLPAEDFPLVQEAPSFGTAFKVPQKTLKNLLDQVAFAMAVHDIRYYLNGI
                     LFVAEGRQLSLVATDGHRLAFSSATLDTDVPNKQEVILPRKTVIELQRLLSDASVPEG
                     QDAPMIEMQFAPNQAKFSFGAMEFVTKLVEGKFPDYNRVIPKNHKNSVTLGRAPLLAS
                     LQRTAILTSEKFKGVRLNFEPGTLRVASNNAEQEEAVDELDIDYGGDAIEIGFNVTYL
                     IDALANMSQQEMIKIELQDANSSALVTLPDDTNFKYVVMPMRI"
     gene            complement(1674..3101)
                     /gene="dnaA"
                     /locus_tag="PKV30_08055"
     CDS             complement(1674..3101)
                     /gene="dnaA"
                     /locus_tag="PKV30_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRL30200.1"
                     /translation="MQTEISADVAADAGQNLWPACLDVLAQELPQQQFNTWIKPLTAR
                     VAADQSKLTLFVGNRFKLDWIRAQYGARIAGILESLHGQRVDVELALAPRATPVKPTI
                     PEFPSPAEAAPTAPPAEPMAAPAPDEEASASGAFRSRLNGALTFDTLVEGSANRMARA
                     AALHVAGAPGQLYNPLFIYGGVGLGKTHLMHAVGNQLVQARPGAKVLYIHAEQFVSDV
                     VKAYQRKTFDQFKDRYHSLDLLLIDDVQFFANKDRTQEEFFNAFEALLAKKSHIIMTS
                     DTYPKGLADIHERLVSRFDSGLTVALEPPELEMRVAILVSKAAAEGAEMPEEVAFFVA
                     KNVRSNVRELEGALRKILAYSRFNQKEISIALAKEALRDLLSIQNRQISVENIQKTVA
                     DYYKIKVSDMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVR
                     KIAGERQQLTELNQQLHVLEQTLKG"
     gene            3437..3571
                     /gene="rpmH"
                     /locus_tag="PKV30_08060"
     CDS             3437..3571
                     /gene="rpmH"
                     /locus_tag="PKV30_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRL30201.1"
                     /translation="MKRTYQPSKTRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            3631..4029
                     /locus_tag="PKV30_08065"
     CDS             3631..4029
                     /locus_tag="PKV30_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRL30202.1"
                     /translation="MRHLRTRDQFQALLSERPVARTAHFVLHRLRPELADAGAAARTR
                     LFCAAEVAWIGAMVPKRWARRAVTRNAIRRQIYAVTGSLQPPLPAAVHLVRLRAAFAP
                     AQFPSASSAALKQAVRAELLDLFAQPERRA"
     gene            4026..4313
                     /gene="yidD"
                     /locus_tag="PKV30_08070"
     CDS             4026..4313
                     /gene="yidD"
                     /locus_tag="PKV30_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRL30203.1"
                     /translation="MMRALLVGVVKGYRLLLSPWLGSACRFEPTCSLYAIGALERHGA
                     LAGSYLTLRRLARCHPWCEGGCDPVPTQAPGLFTGLIGAKRSDPHSPRQPS"
     gene            4310..>5837
                     /gene="yidC"
                     /locus_tag="PKV30_08075"
     CDS             4310..>5837
                     /gene="yidC"
                     /locus_tag="PKV30_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRL30204.1"
                     /translation="MNDIRRTILWVIFAFSLVMLWDQWQVYNGHKATFFPSASNVAAR
                     ASAPASAAASATPPAASATAVPAATSTSAPPSGAPAAAALPRERIEVQTDVLKLVFDT
                     EGGAIVKSEFLKYDETGQTNQPFVLLDTSANRVYQAQSGLIGGPFPTHKTPMKFSGER
                     SLADGANELVLRFESPEQGGVRLIKTYTLKRGAYVIDVRHEVVNQSGQAINPQLYVQL
                     VRDGNKPPGESSFYSTFTGPAFYSDAKKFQKVEFSDIEKNKAEFVTSSPDGWVAMVQH
                     YFASAWLLPDGKPRENFARKIDQNLYAAGMITPIGAIAPGATAAVDARLFIGPQEEKV
                     LESIAPGLELVKDYGFFTILSKPLYWLLDKLHGFIGNWGWSIVALVVLLKIAFYWFNA
                     KAYQSMAKMKAINPKVMDMRERYKDNPQQMQQEMMRIYREEKVNPMGGCLPILIQIPV
                     FIALYWVLLSSVEMRGAPWILWIKDLSVRDPYFILPIVMTLTTLLQTALNPLPPDPMQ
                     A"
BASE COUNT          956 a         1888 c         1847 g         1146 t
ORIGIN      
        1 gggctcgtgc ttgtcgtcca tcttcacgcc ggtggggatg ccgcggccgt tgtcgaccac
       61 gctgatcgag ttgtcggagt ggatggtgac gacgatgtcg tcgcaatagc cggccagtgc
      121 ctcgtcgatc gagttgtcga ccacctcgaa caccaggtgg tgcaggccgg tgccgtcgct
      181 ggtgtcgccg atgtacatgc ccgggcgctt gcgcaccgcc tccaggcctt ccaggatctg
      241 gatggcgcct tcgccgtagc cgccagcgtt ggcgggggtt tgggagtcgg gcgtgggggc
      301 gtcggtcatg aagtcttccg ggcagctgct tttaaatgaa aaatgccttc agcgcttgac
      361 tggcaagcgc gagcagctat caaaaacata gtgtctgtcg atggcgcatc aaatgcgcat
      421 cggcatcacc acatacttga agttggtgtc gtcgggcagg gttacaaggg ccgagctgtt
      481 ggcgtcctgc agctcgatct tgatcatttc ctgctggctc atgttggcca gcgcgtcgat
      541 caaataggtg acgttgaagc cgatctcgat ggcgtcgccg ccatagtcga tgtcgagttc
      601 gtccaccgcc tcttcctgct cggcgttgtt ggacgccacg cgcagcgtgc cgggctcgaa
      661 attcagacgc acgcccttga atttctcgct ggtcaaaatc gcggtgcgtt gcaggctggc
      721 caacagcggc gcgcggccca gtgtgacgct gttcttgtgg ttcttgggga tgacgcggtt
      781 gtagtcgggg aacttgcctt cgaccagctt ggtgacgaac tccatggcgc caaagctgaa
      841 cttggcctgg ttgggcgcaa actgcatctc gatcatcggc gcgtcctggc cttcaggcac
      901 gctggcgtcg gacagcaggc gctgcaactc gatcacggtc ttgcgcggca ggatgacttc
      961 ctgcttgttc ggcacgtcgg tgtcgagcgt ggcgctggaa aacgccagcc ggtgaccgtc
     1021 ggtcgccacc aggctgagct ggcgcccttc ggccacgaac aggatgccgt tcaggtagta
     1081 gcggatgtcg tgcaccgcca tggcaaacgc cacctggtcg agcaggttct tcagcgtttt
     1141 ttgcggcact ttgaaggcgg tgccgaagct gggggcttcc tgcaccagcg ggaagtcttc
     1201 ggccggcagc gtctgcagcg tgaacttgga tttgccgccc ttgaggatca gcttggacgc
     1261 ctgcgattcc aggctcaccg tctggtcggc cggcatggtg cgcaggatgt cgatcaactt
     1321 gcgcgcgccc acggtggtgg aaaagttgcc gctgtcgccg cccagttgcg ccgcggtgcg
     1381 gatctggatt tcaaggtcgc tggtggtcag ctgcaccgtc tcgccgcttt tgcgcagcag
     1441 cacgttggcc agcaccggca gcgtgtggcg acgttcgaca atgcccgcga ccgactgcag
     1501 cgccgacagc agctggtttt gggtggtctt cagaacaatc atgtcttcct cttgatttcc
     1561 gtcttttctg gcgcgcggcg cgtgatttga agcggcccat gaggcagcgg cgtgttgcgc
     1621 gcaggcaacc ccgtattgtc gccgcttttg gggccgttcc ggcccagctg gcctcagcct
     1681 ttcagggtct gctccagcac gtgcagctgc tggttcagct cggtcagttg ctggcgctcg
     1741 ccggcgatct tgcgcactgc gtgcagcacc gtggtgtggt cgcgcccgcc aaacaattcg
     1801 ccgatttcgg gcaggctttt ttgcgtcaac tccttggcca agtacatggc gatctggcgc
     1861 ggccgggcaa tgctggccgg gcgcttcttg ctgtacatgt ccgagacctt gatcttgtag
     1921 tagtcggcca cggttttctg gatgttctcg acgctgatct ggcggttctg gatcgacagc
     1981 aggtcgcgca gcgcctcctt ggccaaggcg atggaaatct ctttctggtt gaagcgcgaa
     2041 taggccagta tcttgcgcag cgcgccctcc agctcgcgta cgtttgagcg cacgtttttg
     2101 gcgacgaaaa acgccacttc ttcaggcatc tcggcacctt cggcggctgc cttgctgacc
     2161 agaatcgcca cgcgcatttc cagttcaggc ggctccagcg ccaccgtcag gcccgaatcg
     2221 aagcgcgaca ccaggcgctc gtgaatgtcg gccagcccct tggggtaggt gtcgctggtc
     2281 atgatgatgt ggctcttctt cgccagcagc gcctcgaaag cgttgaagaa ctcttcctgc
     2341 gtgcgatcct tgttggcaaa gaactgcacg tcgtcgatca gcagcaggtc gagcgagtgg
     2401 tagcggtcct tgaactggtc gaacgtcttg cgctgataag ccttgaccac atccgacaca
     2461 aactgctcgg catggatgta gagaaccttg gcgcccggcc gcgcctggac cagctgattg
     2521 cccaccgcgt gcatcaggtg cgtcttgccc aaaccgacgc cgccgtaaat gaacagcggg
     2581 ttgtacaact ggcccggcgc gcccgccacg tgcagcgccg cagcgcgcgc catgcggttg
     2641 gcagagcctt cgaccagggt gtcaaacgtc agcgcgccat tcaggcggct gcgaaaagcg
     2701 ccggatgccg acgcctcttc gtcgggcgcg ggcgccgcca tgggctcggc aggtggcgcc
     2761 gtcggcgccg cttctgcggg cgagggaaat tcagggatcg taggtttgac gggcgttgcg
     2821 cggggggcaa gcgctaactc aacgtcgaca cgctgaccgt gcagcgactc cagaatcccc
     2881 gcgatgcgcg cgccgtactg ggcgcgaatc caatcgagct tgaagcggtt gccgacgaac
     2941 agcgtcagct tggactgatc ggccgccacc cgcgccgtca ggggcttgat ccaggtattg
     3001 aactgctgct gcggcaattc ttgcgccagc acatccaggc aggcgggcca caggttctgg
     3061 cctgcatcgg ccgccacgtc ggcggaaatt tcagtctgca tgaaggggtt tgaccttgtg
     3121 gataagcgat gccgccgaag ccggctgatt gtaggcggca aggccattat ccacaaggcc
     3181 ggccgcgggc tgcgcgggtg cattgcggca tccagcggct gcggccaaac tcctgcaatg
     3241 aggcggtctt ggacttatcg ccaagatttg cgataatgcc gggttttcct tgctctgttg
     3301 actttcagtc cggtcaggtg gctggtgggc cggtcaaacg cgcggtgggc agctttttgc
     3361 cacagccacc gcgcccggcg aacgccatgc acagtcagcg agcccatccc accatttgcc
     3421 ctaaggacga tttgtcatga aacgcactta ccaaccctcc aagacgcgtc gcgcacgcac
     3481 ccacggtttc ctggtccgca tgaagacgcg cggcggccgc gcggtcatca acgcgcgccg
     3541 tgccaagggc cgcaagcgtc tggctgtctg agccccggcg cagccgtcgg cgtgggtgat
     3601 gcggtcggcg ccgacacgcg cgctgtcgct ctgcgccatc tgcgcacacg cgatcagttt
     3661 caggcgctgt tgtcggaacg ccccgtggcg cgcaccgctc actttgtctt gcatcgcttg
     3721 cgacccgagc tggccgacgc tggcgctgcg gcgcgcacgc ggcttttctg cgccgctgaa
     3781 gtcgcctgga tcggcgccat ggtgcccaag cgttgggcac gccgcgccgt cacgcgcaat
     3841 gccattcgcc gccagatcta cgccgtgaca ggcagcctgc agccgccatt gccggccgcc
     3901 gtgcacttgg tgcgtttgcg cgccgcgttc gcgccggcgc agtttccaag cgccagttcc
     3961 gccgcgctga agcaagccgt gcgcgctgaa ttgctcgact tgttcgcgca acctgaaagg
     4021 cgcgcctgat gcgtgcgttg ctcgtcggcg tggtcaaagg ctatcgcctg ctgctctcgc
     4081 cctggctcgg ctcggcctgc cgcttcgagc ccacctgctc gctgtacgcc attggcgcgc
     4141 tcgagcgcca cggcgcgttg gctggcagct acctgacgct gcgccgcctg gcgcgctgcc
     4201 acccgtggtg cgagggcggc tgcgacccgg tgcccacgca agcgcccgga cttttcaccg
     4261 gtttgatcgg cgccaagcga tccgacccgc attcccctcg tcagccgtca tgaacgacat
     4321 tcgccgcacc attctctggg tgatctttgc cttctcgctg gtcatgctgt gggaccaatg
     4381 gcaggtctac aacggccaca aggccacctt cttcccgagc gcctccaacg tggctgcgcg
     4441 cgccagcgca ccggcttctg ctgctgcctc ggccacccca ccagccgcca gtgccacggc
     4501 cgtgcccgcc gccaccagca cttctgcgcc gccttctggc gcgcctgccg ccgcagcgtt
     4561 gccgcgcgag cgcatcgagg tgcagaccga cgtgctcaag ctggtgttcg acaccgaggg
     4621 cggcgccatc gtcaagagcg agttcctcaa gtacgacgaa accggccaga ccaaccagcc
     4681 tttcgtgctg ctcgacacca gcgccaaccg cgtctatcag gcccagtcgg gcctgattgg
     4741 cgggcccttc cccacgcaca agacgccgat gaagttcagc ggcgagcgca gtctggccga
     4801 tggcgccaac gagctggtgc tgcgcttcga atcgcccgag cagggcggcg tgcgcctgat
     4861 caagacctac acgctcaagc gcggcgccta cgtgatcgac gtgcgtcacg aagtggtcaa
     4921 ccagagcggc caggccatca acccgcagct gtacgtgcaa ctggtgcgcg acggcaacaa
     4981 gccgccgggc gagtcgtcgt tttattccac cttcaccggc ccggcgttct actccgacgc
     5041 caagaagttc cagaaggtcg aattcagcga catcgaaaag aacaaggccg agttcgtcac
     5101 ctcttcgccc gacggctggg tcgccatggt gcagcactac ttcgccagtg cctggctgct
     5161 gcccgacggc aagccgcgcg agaacttcgc gcgcaagatc gaccagaacc tttacgcggc
     5221 cggcatgatc acgcccatcg gcgcaatcgc tccgggcgcc acggcggctg tcgacgcgcg
     5281 cctgttcatc ggcccgcagg aagagaaggt gctggaaagc atcgcccccg gcctggagct
     5341 ggtgaaggac tacggcttct tcaccatcct gtccaagccg ctgtactggc tgctcgacaa
     5401 gctgcacggc ttcatcggca actggggctg gtccatcgtc gcgctggtgg tgctgctgaa
     5461 aattgccttc tactggttca acgccaaggc ctatcagagc atggccaaga tgaaggccat
     5521 caaccccaag gtgatggaca tgcgcgagcg ctacaaggac aacccgcagc agatgcagca
     5581 ggagatgatg cgcatctacc gcgaggaaaa ggtcaacccg atgggtggct gcctgccgat
     5641 cctgattcag atcccggtgt tcatcgcgct gtactgggtg ctgctgtcgt ccgtcgaaat
     5701 gcgcggcgcg ccgtggatct tgtggatcaa ggacttgtcg gtgcgcgacc cgtacttcat
     5761 cctgcccatc gtcatgacgc tcaccacgct gctgcagacc gcgctcaacc cgctgccgcc
     5821 cgatccgatg caggcca
//
LOCUS       DAOMVO010000145         5819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3726_length_5819_cov_15.455413, whole genome shotgun sequence.
ACCESSION   DAOMVO010000145 DAOMVO010000000
VERSION     DAOMVO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_5819_cov_15.455413"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKV30_08080"
     CDS             <1..930
                     /locus_tag="PKV30_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30205.1"
                     /translation="ASTLAVLMVLRFFIGMGLGGELPVASTYVSERVPVHERGRIVVL
                     LESFWALGWILAALIAYFVMPRFDKDMGWRVALLLGALPAVYALYLRRKLPDDKPTAP
                     PSARLPWTERLAALWAPAHRRNTLMLWILWFTVVFSYYGMFLWLPSVMIGKGFALIKS
                     FEYVLLMTLAQLPGYFTAAWLIERMGRKFVLVVYLLGTAASAWAFGNATTEGMLLLFG
                     ALLSFFNLGAWGALYAYSPENYPAPIRASGVGTAAAVGRVGGILGPLTIPYLGAQGWS
                     TGAIFALFAVTIAIGAAAVALLGRETKGELAVS"
     gene            1046..1621
                     /locus_tag="PKV30_08085"
     CDS             1046..1621
                     /locus_tag="PKV30_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRL30206.1"
                     /translation="MSLNLWDYAPEDLEVTVSELLIATAASSDALIDDAVREVLHELR
                     QHLGMDVIFLSEIRGGQRLFKHVDARPGAEVIATGGGAPVEQSFCQFVLGGRLPGLVH
                     DAATHPAAPQLPATPFRVGAHLSTPIVLADGRVCGTLCSFSFAGDESLSEKDLGKLEC
                     VARVAAKRIDTRLRKGGDAELAAWQAPSGGA"
     gene            complement(1801..2871)
                     /locus_tag="PKV30_08090"
     CDS             complement(1801..2871)
                     /locus_tag="PKV30_08090"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRL30207.1"
                     /translation="MNALAHLLGTPFPLLQAPMAGVQGSALAIAVGHAGALGSLPCAM
                     LAPDALRAELQALQASGLPHNVNFFCHTPPEPDAASEAAWRAALKPYYDELGLDAASI
                     PAGPGRRPFDADSAALLAEFKPRVVSFHFGLPAPDLLAQVKSWGSKVLASATTVREAL
                     WLEEHGADAVIAQGLEAGGHRGNFLSMDISEQMGTFALLPQIAATVRVPVIAAGGIAD
                     AAGVRAAMALGAAGVQVGTAYLCADEATTTALHRAALQSEAARHTTLTNVFTGRPARG
                     IVNRVMREMGGSGKTHFATGLSRAAPAFPLATNAMAPLRAAAEKAGRTDFSPLWSGQN
                     ATACLSAPAADITRALAAGFKD"
     gene            2963..5617
                     /gene="uvrA"
                     /locus_tag="PKV30_08095"
     CDS             2963..5617
                     /gene="uvrA"
                     /locus_tag="PKV30_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRL30208.1"
                     /translation="MAKTSNFPQLPGRPNASGGFVAVRGAREHNLKDVDVAIPRDALV
                     VFSGVSGSGKSSLAFGTLFAEAQRRYLESVAPYARRLIDQAGVPDVDAIDGLPPAVAL
                     QQARGVGNARSSVGSVTQLANLVRLMYSRAGAYPAGQPMLYAEDFSPNTPQGACPTCH
                     GLGHVYEVTEELMVPDPSLSIRERAIASWPPAWHGQNLRDILVTLGYDVDKPWRDLPK
                     KDRDWILYTEESPTVPVYPGFTPAQTRAAVKAKLEPGYMGTFTGARRYVLHTFANSQS
                     ALMKRRVARYMEGRLCPTCHGKRLKPEALAVTFAGVDIAQLEQLSLDRVAALLQPIAE
                     GDFGTHAAGAEVGGEATRRDRAERAASGRPTHAAAPDVRRTSALSEEKRLAAQRLAEG
                     VVARLGTLRQLGLGYLTLDRATPTLSTGELQRLRLATQLSALLFGVVYVLDEPSAGLH
                     PADSEALYDALDRLRDAGNSVYVVEHDLELMRRAQWLVDVGPGAGEKGGRVLYSGAPD
                     GLRAVTDSQTARYLFDEMPAPPSRQRAAQGWLELIDIRRHNLHGVDARIPLSTLTAIT
                     GVSGSGKSSLIAQALPELVLAHLGHEPEEEPSSDEAEGPSTIERTHGRLGGDVERIQR
                     LVQVDQKPIGRTPRSNLATYTGLFDPVRKLFAATPEAKRLRYDAGRFSFNVAKGRCET
                     CEGEGFVSVELLYMPSVYAPCPACHGARYNDDTLRVRWQDRSIADVLRMTVDEAHEFF
                     FDEEPVRRPLRLLRDIGLGYLRLGQPATELSGGEAQRIKLATELQRSQRGRTLYVLDE
                     PTTGLHAADTDRLLVQLQRLVDDGNTVVMIEHDMRVVAQADWVIDVGPGAGDAGGRIV
                     AAGTPAKVAKARGSRTAPFLAAELARAE"
     gene            complement(5624..>5819)
                     /locus_tag="PKV30_08100"
     CDS             complement(5624..>5819)
                     /locus_tag="PKV30_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30209.1"
                     /translation="GARIDHAYDDLYYLERACQAQVLAQSTGRPLAPVAADLAARVCA
                     QTLGERLQSELSFEALRRRL"
BASE COUNT          855 a         1980 c         2047 g          937 t
ORIGIN      
        1 gccagcacgc tggcggtgct gatggtgctg cgctttttca tcggcatggg tctcggcggc
       61 gagctgccgg tggccagcac ctatgtgtcc gagcgcgtgc cggtgcacga gcgcgggcgc
      121 atcgtggtgc tgctggaaag cttctgggcg ctgggctgga ttctggcggc gctgatcgcc
      181 tacttcgtca tgccgcgctt cgacaaggac atgggctggc gcgtggcgct gctgctgggc
      241 gcgctgccgg ccgtctacgc gctgtacctg cgccgcaagc tgccggacga caagcccacc
      301 gcgccgcctt cggcccgcct gccatggacc gaacggctgg ccgcgctgtg ggcacccgcg
      361 caccgccgca acacgctgat gctgtggatt ttgtggttca ccgtggtgtt ctcgtactac
      421 ggcatgtttt tgtggctgcc cagcgtgatg atcggcaagg gctttgccct catcaagagc
      481 tttgaatacg tgctgctgat gacgctggcg caactgcccg gctacttcac cgcggcctgg
      541 ctgatcgagc gcatgggccg caagttcgtg ctggtggtgt acctgctcgg caccgcggcc
      601 agcgcctggg cgtttggcaa cgcgacgacc gaaggcatgc tgctgctgtt tggcgcgctg
      661 ctgtccttct tcaacctggg tgcctggggc gcgctgtacg cgtattcgcc cgaaaactac
      721 ccggcgccga tccgcgccag cggcgtcggc acggcggcgg cggtggggcg cgtgggtggc
      781 atcctggggc cgctgaccat tccgtacctg ggcgcgcagg gctggagcac cggcgccatc
      841 tttgcgctgt ttgccgtcac catcgccatc ggcgcggcgg cggtggcact gctggggcgc
      901 gagaccaagg gcgagctggc ggtttcttga gtcgtttggt ttgctccaaa aacaagagct
      961 gctcgcgcgc gctggataag cgaaggcggc ctgttcttta tagaatgggt gcgcggtgtt
     1021 gttgcggcct ttccttcgtc acgccatgag tctgaatttg tgggactacg cgcccgaaga
     1081 ccttgaagtc acggtgagcg aactgctcat cgccaccgcc gccagcagcg acgccctgat
     1141 cgacgacgcc gtgcgcgagg tgctgcacga gctgcgccag cacctgggca tggatgtgat
     1201 ctttctgtcc gaaatccgcg gtggccagcg cctgttcaag cacgtcgatg cccggcccgg
     1261 cgccgaagtc atcgccacgg gaggcggcgc gccggtcgaa caatcgtttt gccagttcgt
     1321 gctgggcggc cgcctgccgg gcctggtgca cgacgccgcc acgcacccgg cagcgccgca
     1381 gttgccggcc acgccatttc gcgttggcgc gcacctcagc acgcccatcg tgctggccga
     1441 cgggcgcgtg tgcggcacgc tgtgctcatt cagctttgcc ggcgacgaat cgctcagtga
     1501 gaaggatttg ggcaagctcg aatgcgtggc ccgcgtggcg gccaagcgca tcgacacgcg
     1561 gctgcgcaaa ggcggcgatg ccgaactggc ggcgtggcaa gccccctccg gcggggcttg
     1621 atcggcgcgg acgaggcttc gaatcggctg tgggagcggg cttgcccgcg atggagcggc
     1681 cggggttatc gaagtgcccc atcgcggcca aggccgctcc cacagtgcgg ggtttggctt
     1741 acgctgctgg ttctattttg atagctactc gcgcttgctg tgcctgcgct ggagcctgat
     1801 tcaatcttta aaacctgcgg ccagcgcgcg cgtgatgtcc gccgccggcg cgcttaggca
     1861 agcggtggcg ttctgcccgc tccacagcgg cgagaagtcg gtgcggccgg ctttttcagc
     1921 cgccgcacgc agcggggcca tggcgtttgt ggccagcgga aaggctggcg ccgcgcggct
     1981 cagtccggtg gcgaaatgcg tcttgccgct gccgcccatc tctcgcatca cccggttgac
     2041 aatgccgcgc gccggccggc cggtgaagac gttggtcagc gtggtgtgac gggctgcttc
     2101 gctttgcagg gcggcgcggt gcaaggctgt ggtggtcgcc tcatccgcgc acagataggc
     2161 cgtgcccacc tgcacgcccg ccgcgcccaa cgccatcgcc gcgcgcacgc cggccgcatc
     2221 ggcaatgccg ccggcggcga tgaccggcac gcgcacggtg gcggcgattt gcggcaacag
     2281 ggcgaaggtg cccatctgct cgctgatgtc catgctcaaa aaattgccgc gatggccgcc
     2341 ggcttccagg ccttgcgcga tgacggcgtc ggcgccgtgc tcctcaagcc atagcgcctc
     2401 gcgcactgtg gtggcggacg ccagcacttt tgatccccag gacttgacct gcgccagcaa
     2461 atcgggcgcc ggcagcccga agtgaaagct gaccacgcgc ggcttgaact cggccagcag
     2521 cgcggcgctg tcggcgtcga acgggcgccg acccgggccg gcggggatgc tggccgcatc
     2581 caggcccagt tcgtcgtaat aaggcttcag ggctgcgcgc caggccgcct cgctcgccgc
     2641 gtcgggctcg ggcggcgtgt ggcagaagaa gttgacgttg tggggcaggc cgctggcctg
     2701 cagcgcctgc aactcggcgc gcagcgcatc gggcgccagc atggcgcaag gcagcgaacc
     2761 cagcgcgccc gcgtggccca ccgcgatcgc gagcgcgctg ccttgcacgc cggccatggg
     2821 cgcttgcagc aagggaaatg gggtgccaag aaggtgtgcc agcgcattca tggcgacgat
     2881 tctggcacgc gcgttgtgga tcggcagccg ggcggtgaat gcagaatcgg tggcatcccg
     2941 gtttcccagc aggcaacact ccatggccaa gacgtcgaac ttcccacagt tgcccggtcg
     3001 cccgaatgcc agcggcggct tcgtcgcagt gcgcggcgcg cgcgagcaca acctcaagga
     3061 tgtggatgtc gccatcccgc gcgatgcgct ggtggtgttc tcgggcgtgt cgggctcggg
     3121 caagtcgtcg ctggcctttg gcaccctctt tgccgaggcg cagcggcgct atctggagtc
     3181 ggtggcgccc tacgcgcggc ggctgatcga ccaggccggc gtgcccgatg tggacgccat
     3241 cgacggcctg ccgcccgccg tggcgctgca gcaggcgcgc ggtgtgggca acgcgcgctc
     3301 gtcggtcggc agcgtcacgc aactggcgaa tctggtgcgg ctgatgtatt cgcgtgccgg
     3361 ggcctacccg gccgggcagc ccatgctgta cgcggaggat ttttcgccca acacgccgca
     3421 gggggcgtgt cccacctgtc acgggctggg ccatgtgtac gaggtgaccg aagaactgat
     3481 ggtgcccgac ccctcgctct cgattcggga gcgcgccatc gcctcgtggc cgcccgcgtg
     3541 gcacggccag aacctgcgtg acatcctcgt cacgctcggc tacgacgtgg acaagccctg
     3601 gcgcgatttg cccaagaaag accgcgactg gatcctctac accgaagaat cgcccaccgt
     3661 gccggtgtac cccggcttca cgccggcgca gacgcgcgcc gccgtgaagg ccaagctgga
     3721 gccgggctac atgggcacct tcaccggcgc gcggcgctac gtgctgcaca cctttgccaa
     3781 cagccagagc gcactgatga agcgccgcgt ggcgcgctac atggaagggc gcctgtgccc
     3841 cacctgccac ggcaagcgcc tcaagcccga ggcgctggcg gtgacctttg ccggcgtgga
     3901 cattgcgcag ctggagcagc tgtcgctcga ccgcgtggcc gcgctgctgc agccgattgc
     3961 ggagggcgac ttcggcaccc acgccgctgg cgctgaagtc ggtggcgagg ccacccgccg
     4021 cgaccgcgcc gagcgcgccg ccagcggccg ccccacgcac gccgccgcgc ccgatgtgcg
     4081 gcgcacctcg gcgctgtcgg aagaaaagcg cctggccgcc cagcgcctgg ccgaaggcgt
     4141 ggtggcgcgg ctgggcaccc tgcgccaact cggcctgggc tacctcacgc tggaccgcgc
     4201 cacgccgacg ctctccaccg gcgagttgca acgcctgcgc ttggcgacgc aactcagcgc
     4261 gctgctgttc ggcgtggtct acgtgctgga cgaaccttcc gccggcctgc acccggccga
     4321 cagcgaggcg ctgtacgacg cgctggatcg gctgcgtgat gcgggcaatt cggtctacgt
     4381 ggtcgagcac gatctggagc tgatgcggcg cgcgcagtgg ctggtcgatg tcggccccgg
     4441 cgcgggcgag aagggcgggc gcgtgctcta cagcggcgcg cccgacggtt tgcgcgcggt
     4501 gaccgattcg cagacggcgc gctatctgtt cgacgaaatg cccgcgccgc caagccgcca
     4561 gcgcgcggcg caaggctggc tggagttgat cgacattcgc cgccacaacc tgcacggtgt
     4621 agatgcgcgc atccccttaa gcacgctcac ggcgatcacg ggcgtatcag gctccggcaa
     4681 atccagcctg atcgcgcaag cgctgcccga actggtgctg gcgcatctgg gccacgagcc
     4741 agaagaagaa cccagcagcg acgaagccga agggccaagc accatcgaac gcacgcacgg
     4801 gcgcctgggc ggcgatgtgg agcgcatcca gcgcctggtg caggtggacc agaagcccat
     4861 cggccgcacg ccacgctcca acctcgccac ttataccggg ctgttcgatc ccgtgcgcaa
     4921 gctgtttgcc gccacgccag aggcgaagcg cctgcgctac gacgccggcc gcttctcgtt
     4981 caacgtggcc aagggccgct gcgaaacctg cgagggcgaa ggcttcgtca gcgtcgagct
     5041 gctctacatg ccgagcgtat acgcgccatg ccccgcctgc cacggcgcgc gctacaacga
     5101 cgacacgctg cgcgtgcgct ggcaagaccg cagcatcgcc gacgtgctgc gcatgacggt
     5161 ggacgaggcg catgagttct tctttgacga agagcccgtg cgccgcccgc tgcgcctgct
     5221 gcgcgacatt ggcctgggct atctgcgcct gggccagccg gcgaccgaac tctcgggcgg
     5281 cgaggcgcag cgcatcaagc tcgcgactga gttgcagcgc agccagcgag gccgcacgct
     5341 gtacgtgctg gatgaaccca ccaccggtct gcacgccgcc gacaccgacc ggttgctggt
     5401 gcaactgcag cgcctggtcg atgacggcaa caccgtggtg atgatcgagc acgacatgcg
     5461 cgtggtggca caggccgact gggtgatcga cgtggggccg ggcgcgggcg acgcgggtgg
     5521 gcgcatcgtc gccgctggca cgccggccaa ggtggcgaag gcgcggggca gccgcaccgc
     5581 gcctttcctc gccgccgaac tggcgcgcgc tgaatgactc gcctcagagc cggcgcctga
     5641 gcgcctcgaa agacagctcc gactgcagcc gctcgcccag cgtctgcgcg cagacccgtg
     5701 cggccaggtc ggcggccacg ggcgccagcg gcctgccggt cgattgcgcc agcacctgcg
     5761 cctggcaggc gcgctccagg tagtacaggt cgtcgtaggc gtggtcgatg cgcgcgccg
//
LOCUS       DAOMVO010000146         5744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3818_length_5744_cov_12.175426, whole genome shotgun sequence.
ACCESSION   DAOMVO010000146 DAOMVO010000000
VERSION     DAOMVO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3818_length_5744_cov_12.175426"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKV30_08105"
     CDS             <1..552
                     /locus_tag="PKV30_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRL30210.1"
                     /translation="GGRFGDRGDRPGGPGGRFGDRPPREDRGPRLGDTAFRAQRDAVE
                     QAQAQLRKLAAQAHGEALTQLLGAWADRQPDQVPSVQELGRAVSPVVRTSWTRAIGTG
                     AGAADQAAESLLRLEMAAEVPTPADQLDARRALQLKLLTRRNDPPPAQTWGQDVATVL
                     AAPHDEATARRLQNALKGLLRNK"
     gene            827..1303
                     /locus_tag="PKV30_08110"
     CDS             827..1303
                     /locus_tag="PKV30_08110"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRL30211.1"
                     /translation="MSRWYEHKIPPPVIDVAAAGLMWWLARAVPAAQLWPPGAWPLGL
                     GAALGIALAGGLIALAGVLEFHRAHTTVNPMAPQKASALVTTGVYRITRNPMYLGMLL
                     VLAGWAVWLGNAAAFVGLPLFVAVLNLLQIAPEERVLRERFGDAFTRYAARVRRWI"
     gene            1409..3136
                     /locus_tag="PKV30_08115"
     CDS             1409..3136
                     /locus_tag="PKV30_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30212.1"
                     /translation="MSGPDRVEARPRIPQRDPRDSVVPAAQIGAAAPATWDSARIAAN
                     PQKAQTRAMEGFQKAAGVAQGGANARALADAPGNARAAMNRQDIADPKVQKELRSSLL
                     HLGNAIEDQRFDPNTSSKFREQLKALATASPDTETFKGALAHVRAAAQVLDQNQLAPG
                     TKLAFDPERGTHLSSTGLPTIDKAHLDADLYYKTADGKLHVDSSKSSINAVTSEAQHT
                     LKKTDPDDSQMGRQKGWREGGTVAEPRRLGINAMDASKGFNGLMDDRKLGVVKDAVGH
                     PAERSIVLGDRAYSPNELDRIDEAAKKALPGYIEDARKAHVASGQPESTFKPPYGDFV
                     KEKMATPEAAMKSFDIKAGKAVPAPRPAAGFEMPTARQGGLIGGAATFGISTVVAARD
                     GRITGEEAAQIGRQTAVGAGAGALTAAGERAVTPMVDRAVGRTIQTSAERVAATRLGQ
                     SAVTASTTGAVTRTVATRALGSTGVGAVVATGISAYENRDGLARGDSKAIGNVAADAT
                     VAVGSIAAATATGAAIGSVVPVAGTLVGGAIGLAVGVGVAYGAEISGARDWLAGKASK
                     GVDWVKSWF"
     gene            3278..3949
                     /locus_tag="PKV30_08120"
     CDS             3278..3949
                     /locus_tag="PKV30_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30213.1"
                     /translation="MTIYPDEPPTPTSDAIRAWYATLLGAMSRGVRPDQGVFVAPLPP
                     LVASARATDFRTAHWAMDAATDAGEAEEQDHWCARAFFSQCQTHCALLFAGDLAEWDG
                     GAVLWIDGAPMAVPRASDGSSRIDDAGEWLDPHRFAVPLGGLHAHPRATVKLTSHGLG
                     DVRGLWVYDTATHTARTVLPPDDQAWHHPVAAMVGGQLTVYATPQDRQDGRVAWREDA
                     RRVTG"
     gene            complement(3939..4163)
                     /locus_tag="PKV30_08125"
     CDS             complement(3939..4163)
                     /locus_tag="PKV30_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30214.1"
                     /translation="MLGAALGIAQQQAAHPAKAVKARVLTGVEHRVVKQAACLDHIDN
                     GGHVIGVLLANDRHQPVLVDVKLRVLQLSP"
     gene            complement(4342..5145)
                     /locus_tag="PKV30_08130"
     CDS             complement(4342..5145)
                     /locus_tag="PKV30_08130"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRL30215.1"
                     /translation="MFNCHAGLAALCLFAGITAHAHVPFLKPSQFNIENPRLHVESAF
                     TEYPFQADFAMDSPNFALTAPDGTVTPLKASARTKAAVYLEPAIDAAGTYRVSTGVRT
                     GPLYKAVETAGGKLYFADDMKRVSGKPTSMMYYSSADVYLAKGEPAYAPRPRNQGVEI
                     VPLTSPNRVSVGSPLRLTVLQDGQPAPRARVIVAYDNEHYQKRRIEDLYDVENVRASN
                     ITADENGQFSITPTHGGLLYLFVTLHKRVQPDRWESHNAALTLEAALQP"
     gene            complement(5251..>5744)
                     /locus_tag="PKV30_08135"
     CDS             complement(5251..>5744)
                     /locus_tag="PKV30_08135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRL30216.1"
                     /translation="GAWTADQFDPRLNWGDVEWIKNRWGGKIIIKGIMEPEDGRLAVD
                     SGADALIVSNHGGRQLDGAPSAIAALPAIVDAVGKQIEVHMDSGIRSGQDVLKAWALG
                     ARGTYIGRAFLWGLGAAGEAGVSKALQIIHKELDTTMAFCGHTKIGTVDQSIFRPGTY
                     PTA"
BASE COUNT          933 a         1916 c         2000 g          895 t
ORIGIN      
        1 ggcggccgtt ttggtgatcg tggtgaccgc cccggcggcc ccggtggccg ctttggcgac
       61 cgcccgccgc gcgaagaccg tggcccgcgc ctgggcgaca ccgccttccg cgcccagcgc
      121 gatgccgtcg agcaggcgca ggcccaattg cgcaagcttg ccgcgcaagc gcacggcgag
      181 gcgctgaccc aactgctggg cgcctgggcc gaccgccagc ccgatcaggt gccgagcgtg
      241 caagagcttg gccgcgccgt gagccccgtc gtgcgcacgt cgtggacgcg ggccattggc
      301 accggcgccg gcgccgccga ccaggcggcc gaatcgctgc tgcggttgga gatggccgcc
      361 gaggtgccga cgcccgccga ccagctcgac gcgcgccgcg cgctgcagct caaactgctg
      421 acgcgccgca acgacccgcc cccggcccaa acctggggcc aggacgtcgc cactgtgctg
      481 gccgcgccgc acgacgaggc caccgcgcgg cgtctgcaaa atgccctgaa gggcttgctg
      541 cgcaacaagt aggggtgctg gactggcagc cagcccaaaa caacgaccca acacccgttc
      601 gtcctgagct tgtcgaagga cttggtgata cgtgacatgc caagcggctt cgacaggctc
      661 agcccgaacg gatggcgagg tcggggtttt taagggggaa tcgctggggg tgggagcggc
      721 ccaggctgga gcttgtgggg caaaatggcc tccagcgctt gatgcacaag cgcgagcagc
      781 tattcaattc atagtcaccg agcaaacctt aagccgcacc caagccatgt cccgctggta
      841 cgaacacaag atcccgccgc cggtcatcga tgtggccgca gccggcctca tgtggtggct
      901 ggcgcgcgcc gtacccgctg cgcaactctg gccgcccggc gcctggccgc tgggcctggg
      961 cgcggcgttg ggcatcgccc tggcgggcgg cctgatcgcg ctggccggcg tgctcgaatt
     1021 ccatcgcgcg cacaccaccg tcaatcccat ggcgccacag aaggcgagcg cactcgtcac
     1081 caccggcgtc taccgcatca cccgcaaccc gatgtatctc ggcatgctgc tggtgctggc
     1141 cggctgggcc gtgtggctgg gcaacgcggc cgccttcgtc ggcctgccgc tgttcgtggc
     1201 ggtgctgaac ctgctgcaaa tcgcgccgga agagcgcgtg ctgcgggagc gctttggcga
     1261 tgccttcacg cgctacgcgg cgcgggtgcg acgctggatc tgagcccgct ccgcctgact
     1321 ggtaacgatc ctagctggga gtcctccgga ctgttcagcc gcacagtgcg cgcgtccaat
     1381 agggcggtct gaaattgaga ggatgcccat gtccggacct gatcgagtgg aggcgcgccc
     1441 gcgcattccg cagcgcgatc cacgcgactc cgtcgtaccc gccgcacaga tcggcgccgc
     1501 ggccccagcc acctgggaca gtgcccgtat cgccgccaac ccgcaaaagg cacaaacgcg
     1561 cgccatggag ggctttcaga aggcggccgg tgtcgcacag gggggtgcca atgccagggc
     1621 actggccgac gcacccggca atgcgcgcgc agccatgaac cggcaagaca ttgccgatcc
     1681 caaggtccag aaggagctca ggtcgtcgct gctccatctg ggcaacgcga tcgaagacca
     1741 gcgtttcgac cccaatacgt cgagcaaatt tcgcgagcag ctcaaggcgc tggcgaccgc
     1801 cagcccggat accgagacgt tcaagggggc gctggcccat gtgcgtgcgg cggcccaggt
     1861 gctcgaccag aaccaactgg cgccgggcac caagctggcc tttgatcccg agcgcggcac
     1921 ccatctgtcg tccaccggtc tgccgaccat cgacaaagcc catctcgacg ccgaccttta
     1981 ttacaagacg gcggacggaa agctccacgt ggacagctcc aagagcagca tcaatgccgt
     2041 gacctcggaa gcgcagcaca cgctcaagaa gaccgatcct gacgattcgc agatgggcag
     2101 gcaaaagggc tggcgcgagg gtgggaccgt ggccgagccg cggcgcttag gcattaacgc
     2161 catggacgcc agcaagggct tcaatggcct gatggacgac agaaagcttg gcgtggtgaa
     2221 agacgcagtg ggccatcctg ccgagcgctc catcgtcctg ggtgaccgcg cgtactcgcc
     2281 caacgaactg gatcgcatcg acgaggcggc gaagaaggct ctgcccggat acatcgagga
     2341 cgcgcgcaag gcacacgtcg catccggcca gccggagtcg accttcaagc caccctatgg
     2401 agacttcgtc aaggaaaaga tggccacgcc tgaggcggcc atgaagagct tcgacatcaa
     2461 ggcgggcaaa gccgtgccgg ctccgcgacc ggccgccggc ttcgagatgc ccacggcgcg
     2521 acagggcggc ctaattggcg gtgctgccac tttcggcatc tcgaccgtgg tggcggcgcg
     2581 ggacggccgg atcaccggtg aagaagccgc gcagatcggc cgccagacgg cggtgggggc
     2641 tggtgcgggc gcgttgacgg cggccggtga acgtgccgtc acgccgatgg tggatcgggc
     2701 cgtgggcagg acgattcaga cctctgccga gcgagtcgcg gcgacccggc tggggcagtc
     2761 ggcggtcacg gcttcgacca ccggcgcggt gacccgcact gtcgccacgc gagcgctggg
     2821 ctccacgggc gtcggcgctg tcgtcgcgac cggcatctcc gcctacgaga accgcgacgg
     2881 cctggcgcgc ggggactcga aggcgatcgg caacgtggcg gccgacgcga cggtggcggt
     2941 gggctcgatc gcggccgcta cagccacagg cgccgcgatc ggcagcgtcg tgccggttgc
     3001 gggcaccctg gtcggcggcg cgatcggcct ggcggttggc gttggggtgg cctacggtgc
     3061 cgaaatctcg ggcgccagag attggcttgc cggcaaggcc agcaaaggtg tcgactgggt
     3121 caagtcctgg ttctgagaac gggttgcacg ccattggcca aggccggtcg caccgcgctt
     3181 gtcgactgcg cattctcgaa ctttggcgca gccgccggct cgagcggggc gctcgaaggt
     3241 gggatgatgc cgggctcagg tacaccgcgg atgtgacatg acgatttatc ccgacgaacc
     3301 gcccacgccg acctcggacg ccattcgcgc ctggtacgcc acgctgctgg gtgccatgag
     3361 ccgtggcgtg cggccggatc agggcgtgtt cgtcgcaccc ttgccgccct tggtggcgtc
     3421 ggcacgcgcc accgattttc ggaccgccca ctgggccatg gatgccgcca ctgatgcggg
     3481 cgaggcagaa gagcaggatc actggtgcgc gagggccttc ttttcacagt gccagacgca
     3541 ctgcgcactg ctgttcgcag gcgacctggc cgaatgggac ggtggggccg tgctctggat
     3601 cgatggcgcc ccgatggctg tgccgcgcgc ttccgacggg agcagccgca tcgacgacgc
     3661 aggcgaatgg ctggacccgc accgcttcgc ggtgccattg ggcgggttgc atgcgcatcc
     3721 tcgggccacc gtcaagctca cctcccatgg tctgggcgac gtacgaggcc tctgggttta
     3781 cgacaccgca acccacaccg cgcgcaccgt gctgccgccc gacgaccagg cctggcacca
     3841 tcccgtggcg gccatggtcg gcgggcagct gaccgtttac gccacgccgc aggaccggca
     3901 ggatgggcga gtcgcctggc gcgaggacgc gcgtcgcgtc acgggctgag ttgcaagacg
     3961 cgcagcttga cgtccaccag caccggctga tgccggtcat tggccagcag tacgccgatg
     4021 acgtgtccgc cgttgtcgat gtggtcaaga caggcggcct gtttgaccac ccgatgttcg
     4081 acgccagtca ggacgcgagc tttgacggcc ttggccgggt gcgcggcctg ctgctgtgcg
     4141 atgccgagcg ccgcacccaa catggggtgt tgccaccgcc cggcgaacgc tggtcagcgc
     4201 ccgtggcgag gcacgccaac ggccgactgc aggtctgcgc gagcgccgac gccgagcgcg
     4261 cccgtcaccc agcctggacc gcagtcgttt aagccgtcag cgcttgacaa acaagcgcca
     4321 gcagctaccg attgaatagc atcaaggttg cagcgccgct tccagcgtca gcgcggcgtt
     4381 gtggctttcc cagcggtcgg gctgcaccct cttgtgcagc gtgacgaaca ggtacagcag
     4441 gccgccatgc gtcggcgtga tgctgaactg gccgttctcg tcggccgtga tgttgctggc
     4501 gcgcacgttc tcgacgtcgt acaggtcttc gatgcggcgc ttctggtaat gctcgttgtc
     4561 gtaggcgacg atgacgcgcg cccgcggcgc cggctggccg tcttgcagca ccgtcagccg
     4621 cagcgggctg cccacgctta cgcggttggg cgatgtgagc ggaacgatct cgacgccctg
     4681 gttgcgcggc cgcggcgcat aggccggctc gcccttggcc aggtacacgt cggcgctgct
     4741 gtagtacatc atcgacgtcg gcttgccgct cacgcgcttc atgtcgtcgg caaagtagag
     4801 cttgccgccg gccgtctcga cggccttgta cagcggcccg gtgcgcacgc cggtgctgac
     4861 gcggtaggtg ccggcggcgt cgatggcggg ctccaggtag acggcggcct tggtcctggc
     4921 gctggccttc agcggcgtga cggtgccgtc gggcgcggtc agcgcgaagt tcggcgaatc
     4981 catggcgaag tcggcctgga aagggtattc ggtaaaggcc gattcaacgt gcaggcgcgg
     5041 gttctcgatg ttgaactggc tgggcttgag aaagggcacg tgggcgtgcg ccgtgatgcc
     5101 ggcaaaaagg caaagcgcgg ccaggccggc gtggcagttg aacacgggtg atctgtccaa
     5161 tgggaacgaa aaaagcgatg gcatggcgcc atcgcgcaaa tggctggcgg ccgcgcgcgc
     5221 tggcaggcgc ggcggcaagc tggcgcggcc tcaggctgtc gggtaggttc cgggcctgaa
     5281 gatgctctga tccaccgtgc cgatcttggt atgcccgcag aacgccatgg tggtgtcgag
     5341 ttccttgtgg atgatctgca gcgccttgct cacgccggct tcgcccgcag cgcccaggcc
     5401 ccacaggaag gcgcggccga tgtaggtgcc gcgtgcgccg agggcccagg ctttcagcac
     5461 atcctgaccg ctgcgaatgc ccgaatccat gtgcacttcg atctgttttc ccacggcatc
     5521 gacaatggcc ggcagcgcgg cgatggccga cggcgcgccg tccagctggc gcccgccgtg
     5581 gttgctgacg atgagcgcat cggcgcctga atcgacggcc aggcgcccgt cttccggctc
     5641 catgatgccc ttgatgatga tcttgccgcc ccagcggttc ttgatccact ccacgtcgcc
     5701 ccagttcagg cgcgggtcga actggtcggc cgtccaggcg ccca
//
LOCUS       DAOMVO010000147         5734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3830_length_5734_cov_15.959148, whole genome shotgun sequence.
ACCESSION   DAOMVO010000147 DAOMVO010000000
VERSION     DAOMVO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3830_length_5734_cov_15.959148"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PKV30_08140"
     CDS             <1..146
                     /locus_tag="PKV30_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL30217.1"
                     /translation="TSMTVNVGVYAERFNNQGHYIKVTEAQLTYVAIDDAGKPRPVPV
                     QQL"
     gene            complement(213..698)
                     /locus_tag="PKV30_08145"
     CDS             complement(213..698)
                     /locus_tag="PKV30_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL30218.1"
                     /translation="MSKHTAAPPLPFEPEFVEGLRQVFEQRIPFNQVIGLKLFEMAAD
                     HVVGRVDMRPELVGHFSYNRLHGGVISATLDAMGGMAVMAAIGARHMDEPPARRLERF
                     VKLGTIDLRIDYLRQGIGDFFTAHAEVLRLGSRVASTRMEFRGADGTLIATGAAAYIV
                     S"
     gene            complement(713..1234)
                     /locus_tag="PKV30_08150"
     CDS             complement(713..1234)
                     /locus_tag="PKV30_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRL30219.1"
                     /translation="MQLIACTHVAHAQAILDILNHAIVTSTALYDYQPRSLTNMRTWF
                     DAKAAGGFPVIGGVDDAGTLLGFASYGTWRAFPAYKYSVEHSVYVREDQRGKGLARLL
                     MQALIDAARAQGKHVMVGAIDAANAGSIALHEKLGFTPAGTVRQAGFKFGRWLDVAFY
                     QLILDTPAQPLDG"
     gene            complement(1313..2077)
                     /locus_tag="PKV30_08155"
     CDS             complement(1313..2077)
                     /locus_tag="PKV30_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRL30220.1"
                     /translation="MSDILTHQDAGVLTLTFNRVERKNSINVAMYDALATALEKAAGD
                     AATRVVVIQGHETIFSAGNDIEDFLKNKPAGMDSPVFRFLRGIATFPKPLVAAVCGPA
                     VGVGTTLLFHCDLVYAGDNAAFSMPFVNLGLVPEAASSLLVPQMFGYHRAAEALLLGE
                     PFMAEAALEVGLVNRVVPPSEANALAQAQARKLAAKPLSSLIATKQLMKGGQQKAVLE
                     RMGEEGAQFGRMLGEPAAREAMTAFMEKRKPDFSKC"
     gene            complement(2156..3025)
                     /locus_tag="PKV30_08160"
     CDS             complement(2156..3025)
                     /locus_tag="PKV30_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL30221.1"
                     /translation="MTRPSLMSRHPFDEAVALTPVAATAQERGREAPAHFDGQPHPAY
                     ANMVGPFGGVTAAQMLQSVLQHPQLRGEPVALTVNFAAAVADAPFRVDARPVRTNRST
                     QHWTMALLQTDEHGVDSTVITATAVTAARRDTWSAVDSPMPTVARPADIARALPPKRV
                     RWIERYDMRPVVGPIPEQWDGQEADSLTQLWLRDDPPRPLDFLSLAAMADVFFPRVWR
                     RRAVMTPVGTVSMTVYFHASRAELAATGEGYLLGQARSQSFFNGFFDQSAELWNEAGH
                     LLATTHQIVYYKE"
     gene            complement(3092..3994)
                     /locus_tag="PKV30_08165"
     CDS             complement(3092..3994)
                     /locus_tag="PKV30_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL30222.1"
                     /translation="MSAENANFTRLQTPGGGQVAARLFEPDRAPSMQVVIGAAMGVPQ
                     RRYAEFAGWLAGQGVRVLTFDYRGHGDSLKLLQGKSLRHVRATLNDWRADYEAAVQHL
                     HTLAPDVPLLLIGHSLGAQLPGLFERTDHIAGLLGVATGSGYWRHNAPQLRRRALFMW
                     HGLVPVLTPLFGYFPGKRIGAVGDLPAGVIRQWRRWCLHPRYSGADGAAALARYGAVR
                     FPIRALSITDDEMMTLPGTQSLLALYANAPQRIDRVHPAEHGAERIGHLGWFQPRFAD
                     TLWPYTLKALRELAGKSAVAAAGA"
     gene            complement(4077..5285)
                     /locus_tag="PKV30_08170"
     CDS             complement(4077..5285)
                     /locus_tag="PKV30_08170"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRL30223.1"
                     /translation="MTKQVQDAYIVAATRTPIGRSHKGYFRNTRPDDLLAHVLREALK
                     QAPGLDPAAIEDVISGCAIPEAQQGLNVARIGAVLAGYPKSVGGITVNRFCASGLSAV
                     AMAADRIRVGESEVMVAAGVESMSMVPMMGNSPSLSPSIFGNTDNVDDYGIAYGMGLT
                     AEKVAQQWKVSRAAQDEFALESHQRAMKAIASGEFKDEITPVTVTERSVNLKTAEVTQ
                     TQRTVDIDEGPRPDTSLEGLAKLRTVFAARGSVTAGNSSQTSDGAGALILASEAAVKK
                     YGLTPLARFVSYASRGVPPHIMGIGPIEAIPAALKSAGLTKDQIDWYELNEAFAAQSL
                     AVINTLGLDKAKVNPMGGAIALGHPLGATGAIRSATVVHALRRHKLKYGMVTMCVGMG
                     QGAAGIFERV"
     gene            complement(5306..>5734)
                     /locus_tag="PKV30_08175"
     CDS             complement(5306..>5734)
                     /locus_tag="PKV30_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRL30224.1"
                     /translation="MAKVGMSALDSRKLGYLLWSDVIVPHKDELLYVAINEAKAMYRA
                     GYRPPMKRQFAVAGRDGKATIQGQLVNMRDGGFISQHDFYVSSLIANVLTGGDVDPGT
                     RVDEEYLMTLERQAFTTLLGHPKTHERIMGMLSTGKPVRN"
BASE COUNT          925 a         1855 c         1973 g          981 t
ORIGIN      
        1 gcacctcgat gacggtgaac gtgggcgtgt acgccgagcg cttcaacaac cagggccact
       61 acatcaaggt gaccgaggcg cagctgactt acgtggcgat cgacgacgcc ggcaagccgc
      121 gcccggtgcc ggtgcagcag ctctgagcat tttcaagccc aatgagccgc cagcgcttga
      181 ttgacaagcg cgaacagcta tcaatttaat agtcaagaaa cgatgtaagc cgccgcgccg
      241 gtagcgatca gcgtgccatc ggcgccgcga aactccatgc gcgtgctggc cacgcgcgag
      301 cccaggcgca gcacttcggc gtgcgcggtg aagaagtcgc cgatgccttg gcgcagatag
      361 tcgatgcgca agtcgatggt gccgagcttg acgaagcgct ccagccggcg cgcgggcggc
      421 tcgtccatgt ggcgcgcgcc gatcgcggcc atcaccgcca tgccgcccat cgcgtcgagc
      481 gtggcgctga tgacgccgcc gtgcaagcgg ttgtagctga agtggccgac cagctcgggc
      541 cgcatgtcga cgcggcccac cacgtggtcg gccgccatct cgaacagctt caggccaatc
      601 acctggttga acggaatgcg ctgctcgaaa acctggcgca gaccttcgac gaactccggc
      661 tcgaacggaa ggggtggcgc ggcggtgtgt ttgctcatgc ttggttcttg actcaaccat
      721 cgagcggctg cgccggcgtg tcgagaatga gttgatagaa ggccacatcc agccagcgcc
      781 cgaacttgaa gccggcctgc cgcaccgtgc cggcgggcgt aaagccgagc ttttcatgca
      841 acgcgatgct gccggcgttg gcggcgtcga tggcgccgac catcacgtgc ttgccctgcg
      901 cgcgcgcggc gtcgatcagc gcctgcatca gcagccgcgc cagccctttg ccgcgctggt
      961 cctcgcgcac atagaccgag tgttcgacgc tgtacttgta ggccggaaag gcgcgccagg
     1021 tgccgtagct ggcaaagccc agcaacgtgc ccgcgtcatc caccccgccg atcaccggaa
     1081 agccgcccgc cgccttggca tcgaaccagg tgcgcatgtt ggtcagcgag cgcggctggt
     1141 agtcgtacag cgcggtcgag gtgacgatgg cgtggttgag gatgtccaga atcgcctgcg
     1201 cgtgcgcaac gtgggtgcag gcgatgagct gcatgtcggc gggaaaatgc tatttaaaac
     1261 tgagctgctc gcgcttgata cacaagcgct ggcgacctaa atgacctgaa tttcagcact
     1321 tgctgaaatc cggcttgcgc ttctccataa acgccgtcat cgcttcgcgc gcggccggct
     1381 cgcccagcat gcggccgaac tgcgcgccct cctcgcccat gcgttccagc acggcctttt
     1441 gctggccgcc tttcatgagc tgcttggtgg cgatcaggct ggacagcggc ttggccgcca
     1501 acttgcgcgc ttgcgcctgc gccagcgcgt tggcttccga aggcggcacg acgcggttga
     1561 ccaggcccac ttccagcgct gcctcggcca tgaagggctc gcccagcagc aacgcttcgg
     1621 ccgcgcggtg gtagccgaac atctgcggaa ccagcaggct gctggccgcc tcgggcacca
     1681 gccccagatt gacgaaaggc atcgagaacg ccgcgttgtc gcccgcgtaa accagatcgc
     1741 aatgaaacag cagcgtggtg cccacgccca ccgccggccc gcacacggca gccacgagcg
     1801 gcttggggaa ggtggcgatg ccgcgcagga agcggaacac gggcgaatcc atgccggcgg
     1861 gcttgttctt cagaaagtcc tcgatgtcgt tgccggcgct gaagatggtt tcgtggccct
     1921 ggatgacgac gacgcgcgtg gccgcatcgc ccgccgcttt ttcaagcgcg gtggcgagcg
     1981 cgtcgtacat ggcgacgttg atggagttct tgcgctccac gcggttgaag gtgagcgtga
     2041 ggacgccggc gtcttggtgg gtgaggatgt cggacatggt tcttctctgg gtggaaacta
     2101 tgaaataaat agctgcttgc gcctgccaga caagcgctgg cggcctgttt gactgctatt
     2161 ccttgtagta gacgatctgg tgcgtggtcg ccagcagatg gccggcttcg ttccacagct
     2221 cggcgctctg gtcgaagaag ccgttgaaga aggactgcga gcgcgcctgg ccgagcaagt
     2281 agccctcgcc cgtcgccgcc agttcggcgc ggctggcgtg gaagtacacc gtcatcgaca
     2341 cggtgccgac aggcgtcatc acggcgcggc ggcgccatac gcgggggaag aacacgtcgg
     2401 ccatcgcggc cagactgagg aaatcaagcg ggcgcggcgg gtcgtcgcgc agccagagtt
     2461 gcgtgaggct gtcggcctcc tggccgtccc actgctcggg aatcgggccg accacggggc
     2521 gcatgtcgta gcgctcgatc cagcgcacgc gcttgggtgg cagggcgcgc gcaatgtcgg
     2581 ccgggcgcgc cacggtgggc atgggcgaat cgacggcgct ccaggtgtcg cgccgcgcgg
     2641 ccgtgaccgc cgtggcggtg atgacggttg aatcgacgcc atgctcatcc gtttgcagca
     2701 gcgccatcgt ccagtgctgg gtggatcggt tggtgcgcac gggccgcgcg tcgacgcgaa
     2761 acggcgcgtc ggccaccgcg gcggcgaagt tcacggtgag cgccaccggc tcgccccgca
     2821 gctgcggatg ttgcagcacg ctttgcagca tctgcgcggc cgtcacgccg ccgaaggggc
     2881 cgaccatgtt ggcgtaggcg gggtgcggct ggccgtcgaa gtgggccggc gcctcgcgcc
     2941 cgcgctcttg cgcggtggcg gccacgggcg tgagcgcgac ggcttcgtcg aaggggtgcc
     3001 gggacatcag gctggggcgc gtcatcggtg gcgtggctcg agtggatggg ctgcgcgtga
     3061 cattagcatg ggtcggcgcg agcgctgtcg ctcaggcgcc tgcggcggcc acggccgact
     3121 tgccggcaag ttcgcgcaat gccttgagcg tgtacggcca cagcgtgtcc gcaaagcgag
     3181 gctgaaacca gccgaggtga ccgatgcgct cggcgccgtg ctcggccggg tgcacgcggt
     3241 cgatgcgctg cggcgcgttg gcgtacaagg ccagcaggct ttgcgtgccg ggcagcgtca
     3301 tcatctcgtc gtcggtgatc gacagcgcgc gaatgggaaa gcgcaccgcg ccatagcgcg
     3361 ccagcgcggc cgcgccatcg gcgccgctgt agcgcgggtg caggcaccag cggcgccact
     3421 ggcggatcac gccagccggc aagtcgccca cggcaccaat ccgcttgccg ggaaagtagc
     3481 cgaacagtgg tgtcagcacg ggcaccaggc cgtgccacat gaagagcgcg cgacgccgca
     3541 actgcggcgc gttgtggcgc cagtagccgc tgccggtggc cacgcccagc aggccggcga
     3601 tgtgatcggt gcgctcgaac aagcccggca gctgcgcgcc caggctgtga ccaatgagca
     3661 gcaggggcac atcgggcgcc agggtgtgca ggtgctgcac ggcggcttcg taatcggcgc
     3721 gccagtcgtt gagcgtggca cgcacatgcc gcaggctctt gccttgcagc agtttcagcg
     3781 aatcgccgtg accacgatag tcgaaggtga gcacacgcac gccctgcccg gccagccacc
     3841 cggcaaactc ggcatagcgc cgctgcggca cgcccatggc ggcgccgata acgacttgca
     3901 tcgatggcgc gcggtccggt tcaaacagac gcgcagcgac ctggccgccg ccgggcgttt
     3961 gcaagcgcgt gaagttggcg ttctccgcgc tcatgatttc agttttgata gcgcttcgcg
     4021 cagatggggc aagcgccaac ggctgaaaac aacgtgatct gagcgcgggc agtcgatcaa
     4081 acccgctcaa aaatccccgc cgcgccctgc cccatgccca cgcacatcgt caccatgccg
     4141 tacttcagct tgtggcggcg cagcgcgtgt acaacggtgg ccgagcggat ggcgcccgtc
     4201 gcgcccagcg ggtggccgag cgcaatcgcg ccgcccatcg ggttgacctt ggccttgtcg
     4261 aggccgagcg tgttgatgac ggcgagcgat tgcgcagcga aggcttcgtt caactcgtac
     4321 cagtcgatct ggtctttggt gaggccggcc gacttgagcg ccgccggaat cgcctcgatc
     4381 gggccgatgc ccatgatgtg gggcggcacg ccacggctgg catagctgac gaagcgcgcc
     4441 agcggcgtca ggccgtattt cttcaccgcc gcttcgctgg ccagaatcag cgcgccggcg
     4501 ccgtcgctgg tctgcgagct attgcccgcc gtcaccgagc cgcgcgcggc gaacacggtg
     4561 cgcagcttgg ccaggccttc cagcgaggtg tcggggcgcg ggccttcgtc gatgtccacg
     4621 gtgcgctggg tttgcgtcac ttcagcggtt ttcaggttga ctgatctttc ggtcaccgtg
     4681 acgggcgtga tctcgtcctt gaactcgccc gaagcaatcg ctttcatggc gcgctggtgc
     4741 gactccagcg cgaattcgtc ctgcgcggcg cgcgagactt tccactgctg cgccaccttc
     4801 tcggccgtca ggcccatgcc gtaggcaatc ccgtagtcgt ccacgttgtc ggtgttgccg
     4861 aagatgctgg gcgacagact gggcgagttg cccatcatgg gcaccatgct catgctttcc
     4921 acaccggcgg cgaccatcac ttcggattcg cccacgcgga tgcgatccgc cgccatcgcc
     4981 accgccgaca ggcccgaggc gcagaagcgg ttgacggtga tgccgcccac gctcttgggg
     5041 tagccggcca gcaccgcacc gatgcgcgcc acgttcaggc cctgttgcgc ctcggggatg
     5101 gcgcagccgc tgatgacgtc ttcgatggcg gcggggtcga gcccgggcgc ctgcttgagc
     5161 gcttcgcgca gcacgtgcgc gagcaggtcg tcggggcgcg tgttgcggaa gtagcccttg
     5221 tgcgagcgcc cgatgggcgt gcgcgtggcg gcgacgatgt aggcgtcttg aacttgtttg
     5281 gtcatgatgt gttcttcctc gccaatcaat tcctcaccgg cttgcccgtg ctgagcatgc
     5341 ccatgatgcg ttcgtgcgtc ttggggtggc ccagcagcgt ggtgaaggcc tggcgctcca
     5401 gcgtcatgag gtattcctcg tccacccgcg tgcccggatc aacgtcgccg ccggtcagca
     5461 cattggcgat caggcttgac acgtagaagt cgtgctggct gatgaaaccg ccgtcgcgca
     5521 tgttgaccag ttggccctga atggtggcct tgccgtcgcg gccggccacg gcaaactggc
     5581 gcttcatggg cgggcgatag ccagcgcggt acatcgcctt ggcttcgttg atggcgacgt
     5641 agagcagctc gtccttgtgc ggcacgatca cgtcgctcca cagcaggtag ccgagcttgc
     5701 gcgagtcgag cgcgctcatg ccgaccttgg ccat
//
LOCUS       DAOMVO010000148         5705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3857_length_5705_cov_15.189381, whole genome shotgun sequence.
ACCESSION   DAOMVO010000148 DAOMVO010000000
VERSION     DAOMVO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_5705_cov_15.189381"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1199)
                     /locus_tag="PKV30_08180"
     CDS             complement(105..1199)
                     /locus_tag="PKV30_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL30225.1"
                     /translation="MTGFAAPSPAIRASGLEGATHSMLGLGALVDEMAGQGIAAAELL
                     QGTGLVPQQLADPKALMSTTQKVTVLRNVQRLSAVPDVGLRAGCRQRLSDFGVWGYAL
                     ASSATFGDAVVLGIRHIKLAGPVLEKRFRVEGDRAIFEGCDVLQLGDILPLSSEFWFS
                     SILKLGQTLLGSPGPLPGSLLRLPYPRPAHAAAYERVFGGAIEFDAGAMEWHFDAAAL
                     RQPLPNANPITAGLCAQFCERMVESLPEEEDSLARRIRAACLSSRGQFPSADEMAQRL
                     GLSLRTLQRRLADAGKSYQAIIDEVRASLALEFLEHTSLPVAEIAARTGFSEVASFRK
                     AFRKWTGNAPSHYRERAGDPPSLPSPVRSP"
     gene            1371..2837
                     /locus_tag="PKV30_08185"
     CDS             1371..2837
                     /locus_tag="PKV30_08185"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRL30226.1"
                     /translation="MNDRLAATAAPPARHAALFERQRRAFLQAPCPSAAQRRDDLRRL
                     KRELLRHQDVLAEAMSQDFGYRAPAESKMLDILGSTLEINHALRHLGRWMKPRRRSTE
                     LLFLTNRLRVTYQPKGVVGVIVPWNFPIYLALGPLAAALAAGNRVMVKMPEATPATNA
                     CLRRLLAEAFSEDQVAVVGEELLDPGAFTALPFDHIVFTGSPAVGRTVMRTAAEHLTP
                     VTLELGGKSPAVVLPGADVAEAATRIVHGKAANCGQICVAPDYALVPRASVKAFAQQA
                     QAAFARLYGQRVQGHPDYTALVNERHRQRIAALLDDARAQGATVIACAEGGTGRQMPL
                     HIVTGLTPRMRIAQEEIFGPILPVLAYDTLDDAIAIIQGGPRPLALYAFGHDAAARRR
                     LLAHTHSGGVTVNDWGWHVLNHDAPFGGVGNSGMGTYHGEEGFRELSHARTVFLRHRF
                     FPIGLFYPPFGNAVQRLVLRLWLGAGDPALQTPRKNTP"
     gene            2834..>3300
                     /locus_tag="PKV30_08190"
     CDS             2834..>3300
                     /locus_tag="PKV30_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRL30227.1"
                     /translation="MNTLEEFDYVISGAGSAGCALAARLSEDPGTSVCLLEAGGPDKS
                     VLIHAPAAVVAMLPTKINNYGYDTVPQPGLNGRRGYQPRGRVLGGSSSINAMLYVRGN
                     RWDYDHWAALGNPGWSYDEVLPLFRRSEHNEQFTDAFHGQGGPLNVTYPRHDSP"
     assembly_gap    3301..3400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3401..3709
                     /locus_tag="PKV30_08195"
     CDS             <3401..3709
                     /locus_tag="PKV30_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRL30228.1"
                     /translation="SSPFDGIRGPMLYPTRLDDPAGMAADIRRRADTQYHPVGTCRMG
                     PASDPMAVVDAQLRVHGIAGLRVADASIMPTLIGGNTNAPAIMIAEKAADLIRAHAPL
                     "
     gene            3738..5684
                     /locus_tag="PKV30_08200"
     CDS             3738..5684
                     /locus_tag="PKV30_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRL30229.1"
                     /translation="MRIRHTLVLAALGALPLAHAHEPQPTTPATAQANRAVAAAYDLA
                     QPQDLEDASRGLVATLPGGVIRDAQGNIAWDGGQFAFIRGAAPDSVNPSLWRQEQLNN
                     QAGLFKVTDGIWQIRGYDLANMTLVQGKTGWIVIDPLLTAETAKATLAFAMQQLGHKP
                     VVAVIYTHSHVDHFGGVRGVVDEADVRAGKVSIIAPEGFMEHAVAENVLAGNAMTRRA
                     QYQFGTPLAFGERGAVGSGLGKTVSVGSMGLIPPTDTVRQTGEERVVDGVRIQFLMAN
                     GSEAPAEFAFYFPDLKALCLSEVVTANMHNIYTIRGAQMRDALGWSKYINRMIDAFPQ
                     AQVAFRSHHWPVWGADRIRQHLAHQRDAYRFIHDQAVGLLNQGRKMDDIANATFFPQG
                     LKNDFSTHGYYGTLSHNLRAVYNFYLGYYDANPATLDPLPVAESSQRYVKAMGGIDAV
                     LKQARTAFDAGDYRWAAVLGNHAVMAEPGNEAARMQQAATLEQMGYQAESGVWRNAYL
                     TGAQELRQGAKPVRLSTQGPDMVRGMTLEMIFDFLAVRLDRQKVDGLTLATQVNFTDA
                     NETYALELSNAVLNNTKGRQLSHPDATLSLTRAAFFKMLLAKVPLPKLIEAGEAKIEG
                     NPKALGAVLTNLMEFDPLFNIVTP"
BASE COUNT          912 a         2084 c         1860 g          749 t
ORIGIN      
        1 aaagcccacg agcaggcgga tggggctggc cccccaggac aaggcaggca gagaggcagc
       61 gatggcggcg gaaccgatca gccagtggcg gcggggaagg gacatcatgg actccgcaca
      121 ggggatggaa ggctcggggg atcgcctgcg cgctcccggt agtggctggg cgcgttgccc
      181 gtccatttgc gaaacgcctt gcgaaagctc gccacctcgg aaaacccggt ccgggctgcg
      241 atttccgcca ccggcagcga ggtgtgctcc aggaactcca gggccagcga ggcgcgtacc
      301 tcgtcgatga tagcttggta gcttttgccc gcgtcggcca gccgccgctg caaggtgcgc
      361 agcgacaggc ccaggcgctg ggccatctcg tcggcgctgg ggaactgccc ccggctgctc
      421 aggcaggcgg cgcggatgcg gcgcgccagg ctgtcctcct cctcgggcag gctctccacc
      481 atgcgctcgc agaactgcgc gcacaggccc gccgtgatcg ggttggcgtt gggcaagggc
      541 tggcgcagcg cggcggcgtc gaagtgccac tccatcgccc cggcgtcgaa ctcgatggcg
      601 ccgccgaaca cgcgctcgta ggccgccgca tgggccgggc gcgggtaggg cagccgcagc
      661 aggctgccgg gcagcggccc cggactgccc agcaaggtct ggccgagctt caggatggag
      721 ctgaaccaga actcgctgga cagcggcagg atgtcgccca gttgcagcac gtcgcagccc
      781 tcgaagatgg cgcggtcgcc ctcgacgcga aagcgcttct ccagcaccgg gccggcgagc
      841 ttgatgtggc gtatgcccag caccaccgca tcgccaaagg tggcgctgct ggccagggcg
      901 tagccccaga cgccgaaatc gctcaggcgc tggcggcagc cggcgcgcag gcccacgtcg
      961 ggcacggcgg acaggcgctg cacgttgcgc agcaccgtga ccttctgcgt ggtggacatc
     1021 agggccttgg ggtcggccag ttgctgcggc accaggcccg tgccttgcag cagctcggcg
     1081 gcggcgatgc cctggccggc catttcatcg accagggcac ccaggccgag catgctgtgg
     1141 gtggcaccct ccagtccgct ggcccggatg gccggggaag gcgcggcgaa ccctgtcatc
     1201 gtgaacctcg ttgcatcttg atacgcgcat tgtactcctg gccgcgcacc cccaggtaaa
     1261 ccccgaaagg cgcgtgtttg gcgcgtttgc cccccggcat ggcgtctttg gccctcgcgc
     1321 cgggccggtg cgctgccgac actggcggcc aaaacacacc ggagacacgc atgaacgacc
     1381 gccttgcggc cacggcagcc ccgccagcgc gccacgccgc cctgttcgag cgccagcggc
     1441 gcgccttcct tcaagcgccc tgccccagcg ccgcccagcg ccgggacgac ctgcgccgcc
     1501 tcaagcgcga actgctgcgc caccaggacg tgctggccga ggccatgagc caggacttcg
     1561 gctaccgcgc accggccgaa tccaagatgc tcgacatcct gggctcgacg ctggagatca
     1621 accacgccct gcgccacctc gggcgctgga tgaagccgcg caggcgctcg accgaactgc
     1681 tgttcctgac caaccgcctg cgcgtgacct accagcccaa gggcgtggtc ggcgtgatcg
     1741 tgccctggaa ctttcccatc tacctggcgc tggggccgct ggccgccgcg ctggccgcgg
     1801 gcaaccgggt gatggtgaag atgcccgagg ccacgcccgc caccaacgcc tgcctgcgcc
     1861 gcctgctggc cgaggcgttc agcgaggacc aggtggcggt ggtgggcgag gaactgctcg
     1921 atccgggcgc cttcaccgcc ctgcccttcg accacatcgt gttcaccggc tcgcccgccg
     1981 tgggccgcac cgtgatgcgc accgccgccg agcacctgac gccggtgacg ctggagctgg
     2041 gcggcaagtc gcccgccgtg gtgctgcccg gcgccgacgt cgccgaggcg gccacgcgca
     2101 tcgtccacgg caaggcggcc aactgcgggc agatctgcgt cgcgcccgac tacgcgctgg
     2161 tgccccgcgc cagcgtgaaa gccttcgccc agcaggcgca ggccgcgttc gcccgcctgt
     2221 acggccagcg cgtgcagggc caccccgact acaccgcgct ggtcaacgag cgccaccgcc
     2281 agcgcatcgc cgcgctgctg gacgacgcgc gcgcccaggg cgccacggtc atcgcctgcg
     2341 ccgagggcgg cacgggccgg cagatgccgc tgcacatcgt gaccggcttg acgccgcgca
     2401 tgcgcatcgc gcaggaggaa atcttcggcc ccatcctgcc ggtgctggcc tacgacacgc
     2461 tggacgacgc catcgccatc atccagggcg gcccgcgccc gctggcgctg tacgccttcg
     2521 gccacgacgc ggcggcgcgc cggcggctgc tggcgcacac gcactcgggc ggcgtcacgg
     2581 tcaacgactg gggctggcac gtgctgaacc acgacgcgcc cttcggcggc gtgggcaact
     2641 cgggcatggg cacctaccac ggcgaggagg gcttccgcga gctttcgcac gcgcgcaccg
     2701 tgttcctgcg ccaccgcttc tttcccatcg gcctgttcta cccgcccttc ggcaacgccg
     2761 tgcagcgcct cgtgctgcgg ctgtggctgg gcgcgggcga cccggcgctg caaacccctc
     2821 gaaaaaacac cccatgaaca cccttgaaga attcgactac gtcatctccg gcgcgggctc
     2881 ggcgggctgt gcgctggccg cccgcctgtc cgaagacccc ggcaccagcg tctgcctgct
     2941 ggaagcgggc ggccccgaca agagcgtgct gatccacgcc ccggcggcgg tggtggcgat
     3001 gctgcccacc aagatcaaca actacgggta cgacaccgtg ccgcagccgg gcctgaacgg
     3061 gcggcgcggc taccagccgc gcggcagggt gctgggcggc tccagctcca tcaacgccat
     3121 gctctacgtg cgcggcaacc gctgggacta cgaccactgg gccgcgctgg gcaaccccgg
     3181 ctggtcgtac gacgaggtgc tgccgctgtt caggcgcagc gagcacaacg agcagttcac
     3241 cgacgccttc cacggccagg gcggcccgct gaacgtgacc tacccgcgcc acgacagccc
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccagcccct tcgacggcat
     3421 tcgcggcccg atgctgtacc ccacacgcct cgacgacccc gccggcatgg ccgcagacat
     3481 ccgccgccgc gccgacaccc agtaccaccc cgtgggcacc tgccgcatgg gccccgccag
     3541 cgacccgatg gcggtggtgg atgcgcaact gcgcgtgcac ggcatcgccg gcctgcgcgt
     3601 ggccgacgct tccatcatgc ccacgctgat cggcggcaac accaacgccc ccgcgatcat
     3661 gatcgccgag aaggccgccg acctgatccg cgcacacgcc ccgctttgaa accacacacc
     3721 aaccaggaga catcaccatg agaatccgcc acaccctcgt tctggccgcg ctgggcgcgc
     3781 tgccgctcgc ccacgcccac gaaccgcagc ccaccacgcc cgccaccgcc caggccaacc
     3841 gcgccgtcgc cgccgcctac gatctggccc agccgcagga cttggaggac gcctcgcgcg
     3901 gcctggtagc cacgctgccc ggcggggtga tccgcgacgc ccagggcaat attgcctggg
     3961 acggcgggca gttcgccttc atccgggggg ccgcgcccga cagcgtcaac cccagcctgt
     4021 ggcgccagga acagctcaac aaccaggccg gcctgttcaa ggtgaccgat ggcatctggc
     4081 agattcgcgg ctacgacctc gccaacatga cactggtgca gggcaagacc ggctggatcg
     4141 tgatcgaccc gctgctgacc gccgagacgg cgaaagccac gctggccttc gccatgcagc
     4201 aactgggcca caagcccgtg gtggccgtga tctacacgca cagccacgtg gaccacttcg
     4261 gcggcgtacg cggcgtggtc gatgaagccg acgtgcgcgc aggcaaggtg tccatcatcg
     4321 cgcccgaagg cttcatggag cacgccgtgg ccgagaacgt gctggccggc aacgccatga
     4381 cgcggcgcgc ccaataccag ttcggcaccc cgctggcctt tggcgagcgc ggcgcggtcg
     4441 gctcgggcct gggcaaaacc gtctccgtgg gctccatggg cctgatcccg cccaccgaca
     4501 ccgtgcgcca gaccggcgag gagcgcgtgg tggacggcgt gcgcatccag ttcctgatgg
     4561 ccaacggctc ggaagccccg gcggaattcg ccttctactt ccccgacctg aaggcgctgt
     4621 gcctgtccga ggtggtcacg gccaacatgc acaacatcta caccatccgc ggcgcgcaga
     4681 tgcgcgatgc gctgggctgg agcaagtaca tcaaccgcat gatcgacgcc ttcccgcagg
     4741 cgcaggtggc cttccgcagc caccactggc cggtgtgggg cgcggaccgc atccgccagc
     4801 acctggcgca ccagcgcgac gcctaccgct tcatccacga ccaggccgtc ggcctgctca
     4861 accaggggcg caagatggac gacatcgcca acgccacctt cttcccgcag gggctgaaga
     4921 acgacttctc cacccacggc tactacggca ccctcagcca caacctgcgc gccgtctaca
     4981 acttctacct gggctactac gacgccaacc ccgccacgct ggacccgctg ccggtggcgg
     5041 aatcgtcgca gcgctacgtg aaggccatgg gcggcatcga cgccgtgttg aagcaggcgc
     5101 gcaccgcctt cgacgcgggc gactaccgct gggccgccgt gctgggcaac cacgccgtga
     5161 tggccgagcc gggcaacgag gccgcccgca tgcagcaggc cgccacgctg gagcagatgg
     5221 gctaccaggc cgaatcgggc gtgtggcgca acgcctacct gacgggcgcg caggagctgc
     5281 gccagggcgc caagcccgtg cgcctgagca cccaggggcc ggacatggtg cgcggcatga
     5341 cgctggagat gatcttcgac tttctcgccg tgcggctgga ccgccagaag gtggacggcc
     5401 tgacgctggc gacgcaggtc aacttcaccg atgccaacga aacctacgcg ctggagctgt
     5461 cgaacgcggt gctcaacaac accaagggcc gccagctctc ccacccggac gccacgctgt
     5521 cgctcacgcg cgcggccttc ttcaagatgc tgctcgccaa ggtgccgctg cccaagctga
     5581 tcgaggcggg cgaggcgaag atcgagggca accccaaggc gctgggggcc gtgctgacga
     5641 acctgatgga gttcgacccc ttgttcaaca tcgtcacgcc gtgacggggt gcgctacata
     5701 aaaaa
//
LOCUS       DAOMVO010000149         5696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3867_length_5696_cov_16.100514, whole genome shotgun sequence.
ACCESSION   DAOMVO010000149 DAOMVO010000000
VERSION     DAOMVO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_5696_cov_16.100514"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKV30_08205"
     CDS             complement(<1..379)
                     /locus_tag="PKV30_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRL30230.1"
                     /translation="MKNPVSRFRLATLPLALCCALPALAQTPATETVAQLSAPQLPET
                     VVTAIRTVQPLTDVLADVTLLDEAQMQRSGAVNIADLLQRQPGLELARNGGPGTATSL
                     FMRGGENRFTAVYIDGVRVDTQSG"
     regulatory      complement(473..738)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            818..1069
                     /locus_tag="PKV30_08210"
     CDS             818..1069
                     /locus_tag="PKV30_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HRL30231.1"
                     /translation="MTNPPLIEQIAERVERLLVRHDELRRTNDLLTQQVQALTHERDN
                     LKSRLMAARARVDSLIDRLPAASALLPGESAQGDKESTA"
     gene            1066..1395
                     /locus_tag="PKV30_08215"
     CDS             1066..1395
                     /locus_tag="PKV30_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRL30232.1"
                     /translation="MKQIEVKIMGQAYLLGAPEGGEERLQQAVQRVDAAMCKIRDAGK
                     IKARDRIAVLAALNLAFELAEQPKVAAAPASANADVPALSVAEQATLGALLRRLDQAL
                     GDDGRLL"
     gene            1960..2784
                     /locus_tag="PKV30_08220"
     CDS             1960..2784
                     /locus_tag="PKV30_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL30233.1"
                     /translation="MPIEPLLILELAALGLVTGFLAGLLGIGGGMIMVPFISAILSGR
                     GVEPGLAVKMAIATSMATIIFTSVSSVRAHHKKGAVRWDIVKALAPGIVLGAMVASLG
                     VFALLKGASLYFVFAAFVAFSGSQMFLDKKPSPTRQLPGTAGLLGAGGVIGALSGLVG
                     AGGGFVSVPFMTWCNVAIHNAVATSAALGFPIALANVVGYVIAGQGLQGVPPYSFGYL
                     WLPALVVIASCSVLMAPLGARAAHALPVKKLKRAFAVVLFTLAIYMLYKGMLAVGA"
     gene            complement(2865..3551)
                     /locus_tag="PKV30_08225"
     CDS             complement(2865..3551)
                     /locus_tag="PKV30_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL30234.1"
                     /translation="MQTSLAVTALVMGLAGGPHCIAMCGAACAGIGQAAAPRSTQAIT
                     MFQLGRVVGYSALGALAAATMQGLGWLTVQSAALRPAWTMVHVAAVLMGLMLLVQARQ
                     PVWLETGARRVWARVRAATQSMGLAAPLGLGVAWALLPCGLLYSALLVAALAGSAAGG
                     ALVMALFALGSGVSLWAGPWLLLRLGQNGRGAWSMRVAGLALMLTSAWGLYMGLVHDQ
                     APWCVVPSTH"
     gene            complement(3666..5048)
                     /gene="hemN"
                     /locus_tag="PKV30_08230"
     CDS             complement(3666..5048)
                     /gene="hemN"
                     /locus_tag="PKV30_08230"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRL30235.1"
                     /translation="MPAITPELLTKFDVAGPRYTSYPTADRFVEAVGEPGYLQALDQR
                     RTGPAAMALPLSLYVHIPFCESLCYYCACNKIITKHHERSAPYLRYLSKEVDLHVQHI
                     GAGQSVSQLHLGGGTPTFFSDDELSELMAMLRRNFNLVPGGEYSIEVDPRTVTAERLA
                     HLARLGFNRLSFGVQDFDPIVQKAVHRVQPAEQVFALVEAARGIGFESINVDLIYGLP
                     KQTPESFDRTLAQVAELRPDRIALYAYAHLPERFKPQRRIIAIDLPGAPNKVAMLARS
                     LAAFEQSGYVYVGMDHFALPNDALAVAKRQGRLHRNFQGYSTQPDCDLIGLGVSAIGK
                     VGATYSQNAKSLEEYQDLLDQGHLPVVRGLAVSRDDLLRRAVIMALMCQGELQFESIE
                     LAHLIDFQDYFAAELDTLRGMQDQGLVTVSDSAIQVTPMGWFFVRGVAMVFDKYLQAD
                     RTRARFSKII"
     gene            5230..>5696
                     /locus_tag="PKV30_08235"
     CDS             5230..>5696
                     /locus_tag="PKV30_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRL30236.1"
                     /translation="MTPQTIKVACSNCNLRELCMPLGLSDGDVDKLDDLVTTRRKIKR
                     GAALFSNGDRFNALYAIRTGFFKTCVATADGRDQVTGFQMAGEIIGLDGIVSDRHSCD
                     AVALEDAEVCVMPYDRIEELSREVPALQSHVHKIMSREIVREHGVMLLLGSMRA"
BASE COUNT          952 a         1897 c         1896 g          951 t
ORIGIN      
        1 tgcccgattg cgtatcgacc cgcacgccat cgatgtagac ggcggtgaag cggttctcgc
       61 ccccgcgcat gaacaggctg gtggcggtgc cggggccacc gttgcgcgcc agttccagcc
      121 ccggctggcg ttgcagcaga tcagcgatgt tgacggcgcc gctgcgctgc atttgcgctt
      181 cgtccagcag cgtgacgtcg gccagcacgt cggtcagggg ctgcacggtg cggatggcgg
      241 tgaccacggt ttcaggcagt tgcggggcgc tgagttgcgc cacggtttcg gtggcaggcg
      301 tttgcgccag cgcaggcagc gcgcagcaca gcgccagcgg caaggtggca agccggaatc
      361 gggaaacggg atttttcatg acgggaacac gggtaacaca cccctgccgg cttccccgcc
      421 agcgtctggg gcaaacatgt ccgcgccgcg cgggggccaa cagcccgttg cgcgacgcgt
      481 cctcctcgtt ggccggtatc cgggctggcg aaggtcgctc catggtcgcc tcgccttccc
      541 ggccttgatg acaaggccag tggcagagtg aggctggagc cggcgccccg gcgtgattcg
      601 ccagcgcgcc gttcgcttac cgttgcgggg gcagcgcagg ttaggtgcca atcacggtga
      661 tcggctgccc tcctgcttcc cgttgaactg cggccgtgag agtcacggcg cgcgagcacc
      721 aacgcgggtg attgtacggg tcgaccccgc gccgctcagg cgcccccgtt tcacgcaccc
      781 tacaatcccc aggcttgccc gacccgagtg cgccccgatg acgaacccgc ccctgatcga
      841 acagattgcc gagcgcgtcg agcgcctgct tgtgcgccac gacgaactgc gccgcaccaa
      901 cgacttgctg acccagcagg tgcaggcgct gacgcacgag cgcgacaacc tcaagtcgcg
      961 cctgatggcg gcgcgcgcgc gcgtcgattc gctgatcgac cgcctgccgg ccgccagcgc
     1021 cctgctgccc ggcgagtcgg cccagggcga caaggaaagc acggcatgaa gcagatcgaa
     1081 gtcaagatca tgggccaggc ctacctgctg ggcgcgcccg aaggcggtga ggagcgcctg
     1141 cagcaagcag tgcagcgcgt cgatgcggcg atgtgcaaga tccgcgacgc cggcaagatc
     1201 aaggcgcgcg accgcatcgc ggtgctggcg gcgctgaatc tggcgtttga gctggctgag
     1261 cagcccaagg tcgccgccgc acccgcgtcg gcaaacgccg atgtgcccgc tttgagcgtg
     1321 gccgaacagg cgacgctggg cgcacttctg cggcggctcg atcaggcgct gggcgacgac
     1381 ggcaggctgt tgtagtcggg ggccgctggc cctcacccca accctctccc agagggagag
     1441 ggagaaattc ccgaagcgcc gcgcctcacc tcaccgcatt gcatcgcgct cgatcgactt
     1501 cgctgaaagc aaagaagtgc ccgcggtagc cgcggaactg gctttgccag gccgctggct
     1561 gcgtcccccc ctgcgcagca aaggggggaa gccgcgtcag cggctcaggg ggggtaaaat
     1621 ctgtcctgtc tgcgatgcgc gccgggcttt atatttcctt gaaccaatgc tcatctgagc
     1681 acgggcttgg aacatcgtcc agccagcgtg atcgtctcgc gtcagatgaa cccaaggctg
     1741 gcactgacct cgcccacctg aaccctgcgt tcaggatgac ggtccggcgg cattcgcaga
     1801 caccttattc ggcgttcccc cctgaggcgc tgacgcgcct tcccccctgt cgtacggggg
     1861 gacaacgctg gtggccgggc ccagcccggc cacggcgttt ctgggatggc ctgctccgcg
     1921 gccgtgtgat ttctttgtgt ttctccgccc gcgccgcaaa tgcccattga acccctgctg
     1981 attcttgaac tggccgcgct tggcctcgtc accggctttc tggccgggct gctgggcatt
     2041 ggcgggggca tgatcatggt gccgttcatc tcggccatcc tgagcgggcg cggggtggag
     2101 ccggggctgg ccgtcaagat ggcgattgcg acgtcgatgg cgacgatcat cttcacctcg
     2161 gtcagcagcg tgcgcgcgca ccacaagaag ggcgcggtgc gctgggacat cgtcaaggct
     2221 ctggcaccgg gcatcgtgct gggggccatg gtggcgagcc tgggcgtgtt cgcgctgctc
     2281 aagggcgcgt cgctctactt cgtgttcgcg gccttcgtgg cgttttcggg ctcgcagatg
     2341 tttcttgaca agaagccgtc gcccacgcgc cagttgccgg gcacggcggg gctgctgggc
     2401 gcgggcgggg tgatcggtgc gctgtcgggc ctggtgggcg caggcggcgg tttcgtgtcg
     2461 gtgccgttca tgacctggtg caacgtggcc attcacaacg ccgtggccac cagcgcggcg
     2521 ctgggttttc cgattgcgct ggccaatgtg gtgggctacg tgatcgccgg ccagggcttg
     2581 caaggggtgc cgccctactc ttttggctac ctgtggctgc cggcgctggt ggtgattgcc
     2641 agctgcagcg tgctcatggc gccgctgggc gcacgcgcgg ctcatgcgct gccggtgaaa
     2701 aagctcaagc gcgcctttgc ggtggtgctg ttcacgctgg cgatctacat gctctacaag
     2761 ggcatgttgg ccgtgggcgc ttgattgcgt tttagatcaa acagggcgcc agcgcttgtc
     2821 catcaagcgc gagcagctat aaaaacaaga gtatccgccg cctttcagtg cgtcgacggt
     2881 accacgcacc atggcgcctg atcgtgcacc aggcccatgt acagacccca ggcggaggtg
     2941 agcatcaagg ccaggccggc cacgcgcatg ctccaggcgc cgcgcccgtt ctgccccagg
     3001 cgcagcagca gccacgggcc cgcccacagc gatacgccgg aaccgagcgc gaacagcgcc
     3061 atcaccaggg ccccgcccgc cgcgctgccc gccagcgccg ccaccagcag cgccgaatac
     3121 agcaggccgc acggcagcaa cgcccacgcc acacccaggc ccagcggcgc ggccagcccc
     3181 atcgactgcg tggccgcacg cacccgggcc cagacgcggc gcgcgccggt ttccagccac
     3241 accggctggc gcgcctgcac cagcagcatc aagcccatca gcacggcggc cacgtgcacc
     3301 atggtccacg ctggccgcag cgccgccgat tgcacggtga gccaacccag gccctgcatg
     3361 gtggccgccg ccagcgcgcc cagcgccgaa tagccgacca cgcgccccag ttgaaacatc
     3421 gtgatcgcct gcgtgctgcg cggcgccgcc gcctgcccga tgccggcgca ggccgcgccg
     3481 cacatggcga tgcagtgcgg gccgccggcc aggcccatca ccagggcggt cactgcgagg
     3541 gaggtttgca tggcttggat tcaatgacga aatgacgcca ccgctgacgc ggcggcatga
     3601 cgaaatgacg gcctgtccgg tcagttcgtc atccatgccg cgccagcggc gaagtcattc
     3661 tgtcatcaga tgatttttga aaagcgcgcg cgcgtgcggt cggcctgcag gtacttgtcg
     3721 aacaccatcg ccacgccgcg cacgaagaac cagcccatgg gcgtgacttg aatggcgctg
     3781 tcgctcaccg tcaccaggcc ctgatcctgc atgccgcgca gcgtgtccag ctcggcggca
     3841 aagtagtcct gaaaatcgat caggtgcgcc agctcgatcg attcgaactg cagctcgccc
     3901 tggcacatga gcgccatgat gacggcgcgg cgcaacaggt catcgcgcga cacggccaag
     3961 ccgcgcacca cgggcagatg gccctggtcg agcagatcct gatactcctc cagcgacttg
     4021 gcgttctggc tgtaggtggc gcccaccttg ccgatggccg agacgccgag gccgatcaaa
     4081 tcgcaatcgg gctgcgtgct gtagccctgg aagttgcggt gcagccggcc ctggcgcttg
     4141 gccacggcca gcgcgtcgtt gggcagcgcg aaatggtcca tgccgacgta cacataaccc
     4201 gactgctcaa acgccgccag cgagcgcgcc agcatcgcca ccttgttggg cgcgcccggc
     4261 aaatcgatgg cgatgatgcg ccgctgcggc ttgaagcgct cgggcaagtg cgcgtaggcg
     4321 tagagcgcga tgcgatccgg ccgcagctcg gccacctgcg ccagcgtgcg gtcaaacgat
     4381 tcgggtgtct gcttgggcag gccatagatc aagtcaacat tgatggattc aaagccgatg
     4441 ccgcgcgccg cctcgaccag cgcaaacacc tgttcggccg gctgcacgcg gtgcacggcc
     4501 ttttgcacga tggggtcgaa atcctgcacg ccaaaggaga ggcggttgaa gcccaggcgc
     4561 gccaggtgcg ccagccgctc ggcggtcacg gtgcgcgggt cgacctcgat cgagtattcg
     4621 ccgcccggca ccaggttgaa attgcgacgc agcatggcca tcagctccga caactcatcg
     4681 tcgctgaaga aggtgggcgt gccgccgccc aggtgcaact ggctcaccga ctggccggca
     4741 ccgatgtgct gcacgtgcag gtcgacctct ttgctcagat agcgcagata gggcgcgctg
     4801 cgctcgtggt gcttggtgat gatcttgttg caggcgcagt aatagcacag cgattcgcag
     4861 aacggaatgt gcacgtacag cgacagcggc agcgccatgg cggcggggcc ggtgcggcgc
     4921 tggtcgagcg cttgcagata accgggctcg ccaaccgcct cgacaaaacg gtcggctgtc
     4981 gggtaagacg tgtagcgggg acctgcgacg tcgaatttgg tcagcagctc aggggtgatc
     5041 gcgggcattt ttggacattc cttctagcgt ggccgtactg tgaaccgacg gcgcccgcgc
     5101 ttccttgact atcatcaagg tatccgagtt gattcgtcgg gatttgccat agatcaaacc
     5161 gtcgggccaa cccggtcccc tcccctaaaa tggccgtttc cccccggcgc gctgttgacc
     5221 tttgcctcaa tgacacccca aaccatcaaa gtcgcctgct ccaactgcaa cctgcgtgag
     5281 ctgtgcatgc cactgggctt gtcggacggc gatgtggaca agctggacga tctggtcacc
     5341 acgcgccgca agatcaagcg tggcgcggcc ctgttttcca atggcgaccg tttcaacgcg
     5401 ctgtatgcca tccgcaccgg cttcttcaag acctgcgtgg ccaccgcgga cgggcgcgat
     5461 caggtgaccg gctttcagat ggcgggcgaa atcatcgggc ttgacggcat cgtgagcgac
     5521 cgccacagct gcgacgccgt ggcgctggaa gacgccgagg tttgcgtcat gccctacgac
     5581 cgcattgaag aactctcgcg cgaagtgccg gcgctgcaaa gccacgtgca caagatcatg
     5641 agccgcgaga tcgtgcgcga gcacggcgtg atgctgctgc tgggcagcat gcgcgc
//
LOCUS       DAOMVO010000150         5693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3874_length_5693_cov_13.135154, whole genome shotgun sequence.
ACCESSION   DAOMVO010000150 DAOMVO010000000
VERSION     DAOMVO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3874_length_5693_cov_13.135154"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKV30_08240"
     CDS             complement(<1..369)
                     /locus_tag="PKV30_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRL30237.1"
                     /translation="MPTSQKPAAKSVPAKAAAKPAAKSAARKTAAPDEVEELSTKKAA
                     APAKKAGRPAKGAAAAEKSTPAKRGRPGKAGAKEVEDEDLSDIEADLEGEPGAEAAPA
                     EGVVEKVKPLRMKISKAKERA"
     gene            complement(673..2502)
                     /gene="dnaG"
                     /locus_tag="PKV30_08245"
     CDS             complement(673..2502)
                     /gene="dnaG"
                     /locus_tag="PKV30_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRL30238.1"
                     /translation="MSIPQPFIQELLARTDVVEIVGHYVQLKKTGANFSGLCPFHSEK
                     SPSFTVSPSKQFYHCFGCGKNGNAIGFLMDHTGAGFVEVVHDLAQQYGLQVPEDERSP
                     QERARAAQETEKRHTLTDVLARAGEGYKKHLKDSPRAVDYLKKRGLSGQVARDFGLGY
                     APEGWRTLASVFPDYADPLLDESGLVIVGEDDPSTGSGQAKRYDRFRDRIMFPIRSVK
                     GEVIGFGGRVLGDEKPKYLNSPETPVFHKGRELYGLFEARSALREHGFALVTEGYMDV
                     VALAQLGFANAVATLGTACTPEHVQKLFRFTDQVVFSFDGDSAGRRAARKALEAALPF
                     ASDTRSVKFLFLPAEHDPDSFIRTQGSEAFGQAIKDAVPLSRFLLDAAAEDCDMQTAE
                     GRSHFAANARPLWQLLPAGALAQQLLVEIAAQVQIGMHELEQLWGIRRAPQRSYEKDS
                     YSRLSDKRQSPKTFENRPARPLRRPPAGRCTRALQIVLTEPSAWDRLSQDEHVLLCDL
                     PAPLGPLFTWLAGQALDHGPQPWSALREALRGHEHEDWAVEEVQKLPPDIVSEPDELQ
                     GILRTERREQLKRRMDAALAGGDMARYQALLRELAEPPPAAPA"
     gene            2587..4230
                     /locus_tag="PKV30_08250"
     CDS             2587..4230
                     /locus_tag="PKV30_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRL30239.1"
                     /translation="MPGIFDTDLPRTEANFAPFTPLAFIQRTAEVYPDKLAIVHGDLR
                     QSWGQTYARCRQLANALQQAGIGKNDTVAVMLPNTPPMVEAHFGVPMAGAVLNTLNTR
                     LDPEAVAFMLDHGQAKALIVDPEFASIVKKALALRESKAPLLVIDVEDALYGEGQKIG
                     SITYDPFVAQGDPKFEWKYPADEWDAIALNYTSGTTGNPKGVVYHHRGAATNAVSNIL
                     EWDMPKHAVYLWTLPMFHCNGWCFPWTVAARAAVNVCLRRVEAKAIFDAIRNHGVTHY
                     CGAPIVHGLLVNAPDEMKQGVPAGVKCMVAGAAPPASMIEGMEQMGFDLTHVYGLTEV
                     YGPATVCAKHDAWNELDIGERARLNARQGVRYHLQRDARVLDPTTMQPVPWDGETMGE
                     IMFRGNIAMKGYLKNPKATEEAFAGGWFHSGDLAVQYPDGYIKIKDRSKDIIISGGEN
                     ISSIEVEDVLYRHPAVLAAAVVAKPDERWGETPCAFLELKPGAEVTQADIVAHCKQHL
                     AGFKVPRAIVFGELPKTSTGKIQKFELRKQAGSAAAIDV"
     gene            complement(4298..5209)
                     /locus_tag="PKV30_08255"
     CDS             complement(4298..5209)
                     /locus_tag="PKV30_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30240.1"
                     /translation="MNIETLDLNLLRLFDAIWRRRSVSQAAEELGLSQPAASQGLSRL
                     RHQLGDVLFHRAPGGVQPTPRADQLAVAIQSALSLLEGALEAPADQFRPRESRRVFRL
                     HLSDIGEARFLPALMALLRREAPLTRIETSPLPQPDIAPALHAGRIDLAIGYLREVTD
                     TSMRPLLSDVYVVMLRRDHPMARRWRRLKTAPGHDALGQLEFATVRSHADTLRILHLL
                     GLQQRVRLTAANFLSLPMTVRTTDLAVLMPHSVALGISPQSDFAIFDPVLPMRELTVA
                     MHWSRRFEHDPAQRWLRERIAALFPGQ"
     gene            5293..>5693
                     /locus_tag="PKV30_08260"
     CDS             5293..>5693
                     /locus_tag="PKV30_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HRL30241.1"
                     /translation="MEVSFAHEIESLRLGAGQTFYGEGILAITKGLLQSGVAYVGGYQ
                     GAPVSHLMDVLVQAKGLMAELGVHVEACSNEASAAAMLGASIHYPLRGAVTWKSIVGT
                     NVAADALSNLSSPGVTGGVLIVVGEDYGEGA"
BASE COUNT          928 a         1823 c         1961 g          981 t
ORIGIN      
        1 ggcgcgttcc ttggccttgc tgatcttcat gcgcagcggc ttgacctttt cgaccacgcc
       61 ttcggccggc gcggcctcgg ctccgggctc gccttccagg tcggcctcga tgtccgacag
      121 gtcttcgtcc tcgacctcct tggcgcccgc cttgcccgga cggccgcgct tggccggcgt
      181 cgatttttca gccgcagcgg cgcccttggc ggggcggccg gccttcttgg cgggcgcggc
      241 ggctttcttg gtgctcagtt cttcgacttc gtcgggcgct gccgtcttgc gggcagcgga
      301 cttggccgcc ggcttggcgg cggctttggc gggaacggac ttggcggcgg gcttctggct
      361 ggtcggcatc aatgcacctc aaaggctgtg agcggccatg cgtgcgcggg caagcgcgat
      421 ctggcggctc gggatcaatc aacaaaacgc tttttcacca acgaacgccg cagcggctgg
      481 gctgaccggc gtcggggcaa gatgggtggg cgaacatttg gggtgcagtc cttgcgctag
      541 gtggccgtcc ctggggctgg ggaatcgcgc ccatctgggt cgcgtggccg gttcggaggt
      601 gctgctgtcg ctcttgccgg tgacaagctt tcaattatag cgcgatcgcc tgatttcaag
      661 ccctgtttcg ggctatgcag gcgcagcagg tggcggctcg gccagttcgc gcagcagcgc
      721 ctggtagcgc gccatgtccc cgcccgccag cgcagcgtcc atgcggcgct tgagctgttc
      781 gcgccgctcg gtgcgcaaga tgccctgcag ctcgtcgggc tcggacacga tgtcgggcgg
      841 cagtttttgc acttcctcga cggcccagtc ttcgtgctcg tgcccgcgca gggcttcgcg
      901 cagcgcgctc cagggttgcg ggccgtggtc cagcgcttgg cccgccagcc aggtgaacag
      961 gggccccagc ggcgccggca agtcgcacag cagcacgtgt tcgtcctggc tcaggcgatc
     1021 ccacgcgctg ggctcggtca gcacgatctg cagcgcgcgc gtgcagcgcc cggcgggcgg
     1081 gcggcgcagc gggcgggcgg gcctattttc aaacgtttta gggctctggc gcttgtctga
     1141 caagcgcgag tagctatctt tttcatagct tctttggggc gcacggcgaa tgccccacag
     1201 ctgctccagc tcgtgcatgc cgatctgcac ctgcgccgcg atctcgacca gcaactgttg
     1261 cgccaaggcg ccggcgggca acagttgcca cagcgggcgc gcgttggcgg caaagtgcga
     1321 gcggccctcg gcggtctgca tgtcgcagtc ctccgctgcc gcgtcgagca gaaagcgcga
     1381 cagcggtacc gcgtccttga tggcttgccc gaacgcctcg ctgccttggg tgcggatgaa
     1441 gctgtcgggg tcgtgctcgg ccggcagaaa caagaacttg acggagcgcg tgtcgctggc
     1501 aaagggcagg gcggcttcca gcgccttgcg cgcggcgcgg cggccggctg agtcgccatc
     1561 aaagctgaac accacctgat cggtgaagcg aaacagcttt tgcacgtgct cgggcgtgca
     1621 ggccgtgccc agcgtggcga cggcgttggc aaagccgagc tgcgccagcg ccaccacgtc
     1681 catgtagcct tcggtgacca gcgcaaagcc gtgttcgcgc agcgcgctgc gggcctcgaa
     1741 caagccgtac agctcgcgtc ccttgtgaaa gacgggcgtt tcgggcgagt tcaggtactt
     1801 gggcttttcg tcgcccagca cgcggccgcc gaagccgatg acctcgccct tgacgctgcg
     1861 gatcgggaac atgattcggt cgcggaagcg atcgtagcgc ttggcctgtc ctgagcctgt
     1921 cgaaggatcg tcctcgccca cgatgaccag gccggattcg tccagcagcg gatcggcgta
     1981 gtccgggaac acgctggcca gcgtgcgcca gccttcgggc gcgtagccca ggccgaagtc
     2041 gcgcgccacc tggcccgaca gcccgcgctt cttgagatag tcgacggcgc gcggcgagtc
     2101 cttcaggtgc ttcttgtacc cctcaccggc gcgcgccagc acatcggtga gcgtgtggcg
     2161 tttttcagtt tcttgcgccg cgcgggcgcg ctcttgcggg ctgcgttcgt cctcgggcac
     2221 ctgcaagccg tattgctgcg ccaggtcgtg caccacctcg acaaagccgg cgcccgtgtg
     2281 atccatcaga aagccgatgg cgttgccgtt cttgccgcag ccgaagcagt gatagaactg
     2341 cttggacggg ctgacggtga acgagggcga cttttcgctg tggaacgggc acaggcccga
     2401 aaaattggcg cccgttttct tgagctgcac gtagtgcccg acgatctcga ccacgtcggt
     2461 gcgggccaga agctcctgga tgaacggttg cgggatgctc accggtcgat tgtggcgcaa
     2521 gccaactttc agtccgccgg cagcgcgctg cctagaatgg tttgcacaac acacaggaga
     2581 cacgcaatgc ccggcatttt cgacaccgac ctgccacgca ccgaggcgaa tttcgccccc
     2641 ttcacaccgc tggctttcat ccagcgcacg gccgaggtgt acccggacaa gctggccatc
     2701 gtgcatggcg atctgcgcca aagctggggc cagacctacg cgcgctgccg ccagctggcc
     2761 aacgccttgc aacaggccgg catcggcaag aacgacaccg tggccgtgat gctgcccaac
     2821 acgccaccga tggtcgaagc gcacttcggc gtgccgatgg cgggcgcggt gctgaacacg
     2881 ctgaacacgc ggctcgaccc cgaggcggtg gccttcatgc tcgaccacgg ccaggccaag
     2941 gcgctgatcg tggatccgga atttgccagc atcgtcaaga aggcgctggc gctgcgcgag
     3001 tcgaaggcgc cgttgttggt gatcgatgtc gaggatgcac tctacggcga gggccagaag
     3061 atcggcagca tcacttacga cccctttgtt gcgcaggggg atccgaagtt cgagtggaaa
     3121 taccccgccg acgaatggga cgctatcgcg ctcaactaca cctcgggcac caccggcaac
     3181 cccaaaggcg tggtctatca ccaccgtggc gcggcgacca atgcggtgag caacatcctc
     3241 gaatgggaca tgcccaagca cgcggtctat ctgtggacgc tgccgatgtt ccactgcaac
     3301 ggctggtgct tcccgtggac ggtggcggcg cgcgcggccg tcaacgtgtg cctgcggcgc
     3361 gtggaagcca aggccatctt cgacgccatt cgaaaccacg gcgtgacgca ctactgcggc
     3421 gcgcccatcg tgcacggcct gctggtgaat gctccggatg agatgaagca gggcgtgccg
     3481 gcgggtgtga aatgcatggt ggcgggcgca gcgccgccgg cctcgatgat cgagggcatg
     3541 gagcagatgg ggttcgacct gacccacgtg tacggcctga cggaggttta cggccctgcc
     3601 accgtgtgcg ccaagcacga cgcgtggaac gaactggaca ttggcgaacg cgcccgcctg
     3661 aacgcgcgcc agggcgtgcg ctaccacctg cagcgcgacg cgcgcgtgct cgaccccacg
     3721 acgatgcagc ccgtgccgtg ggacggcgaa accatgggcg agatcatgtt ccgcggcaac
     3781 atcgccatga agggctacct gaagaacccc aaggcgaccg aggaagcctt tgccggcggc
     3841 tggttccact caggcgatct ggcggtgcag tacccggacg gctacatcaa gatcaaggac
     3901 cgcagcaagg acatcatcat ctcgggcggc gagaacattt cgtccatcga ggtcgaagac
     3961 gtgctgtacc gccacccagc ggtgctggcc gccgccgtgg tggccaagcc ggacgagcgc
     4021 tggggcgaaa ccccctgcgc cttcctggaa ctgaagcctg gcgccgaggt cacgcaggcc
     4081 gacatcgtgg cgcactgcaa gcagcacctg gccggcttca aggtgccgcg cgccattgtg
     4141 tttggcgagt tgcccaagac gtcgaccggc aagatacaga agtttgagct gcgcaagcag
     4201 gccggcagcg cggcggccat tgatgtgtag attgtgccgc tggcgcgcgt ctgtattggt
     4261 gcttaagcta ttgatagagt agcgaacagc caccctgtca ctgacccgga aacagcgccg
     4321 caatccgctc gcgcagccag cgttgggcgg ggtcgtgctc gaagcggcgg ctccagtgca
     4381 tggcgacggt gagttcgcgc atcggcagca ctgggtcgaa gatggcgaag tcgctctgcg
     4441 gcgaaatgcc cagcgccacc gaatgcggca tcagcaccgc gaggtcggtg gtgcgcaccg
     4501 tcatcggcag cgacaagaag ttggcggccg tcaggcgcac gcgctgctgc aagcccagca
     4561 gatgaagaat gcgcagcgtg tcggcgtgcg agcgcacggt ggcaaattcc agttgcccca
     4621 aggcgtcgtg gcccggtgcg gttttcagcc gacgccagcg ccgcgccatg gggtggtcgc
     4681 gccgcaacat caccacgtac acgtcgctga gtagcggccg catcgaggtg tcggtcactt
     4741 cgcgcaggta gccgatggcc aggtcgatgc ggccggcgtg cagcgccggc gcgatgtcgg
     4801 gctgcggcag tggtgaagtc tcgatgcgcg tgagtggtgc ctcgcgccgc agcagggcca
     4861 tcagcgcggg cagaaagcgc gcttcgccga tgtcggaaag atgcaggcgg aacacacgcc
     4921 gtgattcgcg tggcctgaac tgatcggcgg gcgcctccag cgcgccttcg agcagcgaca
     4981 gggccgactg gatcgccacc gccaactgat cggcccgcgg cgtgggttgc acgccaccgg
     5041 gcgcgcggtg gaacagcacg tcgcccaact gatggcgcag gcggctcaag ccctggctgg
     5101 cggccggctg cgacaggccc agctcctcgg cagcctggct cacgctgcgc cgccgccaga
     5161 tggcgtcgaa caggcgcagc aggttcaggt ccagggtttc gatattcatt cagtgcatgt
     5221 tgcttatcac gttcattgta ttgatgaatg tgccgacgcg cgccacactg cgcggcagga
     5281 gacacaacac gcatggaagt ttcgtttgcg cacgagatcg agtcgctgcg cctgggcgct
     5341 gggcagactt tttatggcga gggcatcctg gccatcacca agggcctgct gcagtcgggc
     5401 gtggcctacg tgggcggcta ccagggcgcg ccggtgtcgc acctgatgga cgtgctggtg
     5461 caggccaagg gcttgatggc ggaattgggt gtgcacgtcg aagcctgttc caacgaagcc
     5521 tcggccgctg ccatgctggg cgcgtcgatc cactatccgc tgcgcggcgc ggtgacgtgg
     5581 aagagcatcg tcggcaccaa cgtggcggcc gatgcgctgt ccaacctgtc gtcgcccggc
     5641 gtgaccggcg gcgtgctgat cgtggtgggc gaggactatg gcgagggcgc cag
//
LOCUS       DAOMVO010000151         5654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3918_length_5654_cov_12.697803, whole genome shotgun sequence.
ACCESSION   DAOMVO010000151 DAOMVO010000000
VERSION     DAOMVO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_5654_cov_12.697803"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /gene="rmuC"
                     /locus_tag="PKV30_08265"
     CDS             complement(<1..1411)
                     /gene="rmuC"
                     /locus_tag="PKV30_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRL30242.1"
                     /translation="MNGSSWPDWVFWLALAALALNLLLVLVLLLKRPRRPDDVASRGE
                     VQQLIGAAVGQHGERLERELRQEIGVSAREGRQELSHALATFQDAMVRQGAEGLRTQN
                     AQVDALGAQLTQLRGTLGDTLVQQLQALGLGMAQQAAEATRTQNAQIDAFAQQLAHLR
                     GSLSETLTQQLQQLSETNARRMTEVRGTLEQQLAQLQAANTAKLDEMRQTVDEKLQST
                     LQARLGESFKQVADRLEQVHKGLGEMQTLAQGVGDLKHLLTNVKTRGMFGEAQLGALL
                     EQVFAPEQYAAQVCTRPGSRNAVDFAIKLPGRGAADSGAASAVWLPIDAKFPNEDYER
                     LLDAQQRADAVGAEAAAKGLEARIRLEARGMADKYVEPPHTTDFAILFLPTEGLYAEV
                     LRRPGLMEALQRDHRVTLAGPTTLLAMLNSLQMGFRTLALEKRSSEVWQVLGAVKTEF
                     GKFGGMLDKVKSQTQTVLNT"
     gene            complement(1480..2475)
                     /locus_tag="PKV30_08270"
     CDS             complement(1480..2475)
                     /locus_tag="PKV30_08270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRL30243.1"
                     /translation="MNTAARPQVLVSRAVFPEVIDKLALHFDVEANQADDIWLKDEFI
                     RRLGGKQGVFTTGSERIDAEVLQACPGLKICANMAVGYNNFDVPAMTAAGVLATNAPG
                     VLTETTADMGFALLMAAARRVTEGERFLRDGKWDRWAYDMLIGQDVHGATLGIIGMGR
                     IGQAIARRGALGFGMKVIYHNRSRLPESDEAEVKARYVSKEELLKTADHVVLVVPYSK
                     ESHHTIGAAELALMKPTAVLVNIARGGIVDDVALAKALREGRIAAAGLDVFEGEPKVH
                     PDLLACTNVVLTPHIGSATVPTRQAMANLAADNLIGYLTKGEAVTPLNPEVFARR"
     gene            complement(2503..3012)
                     /locus_tag="PKV30_08275"
     CDS             complement(2503..3012)
                     /locus_tag="PKV30_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRL30244.1"
                     /translation="MTAETETGAISVWRIATDTPDYTADDLSGAGARATGGRWNARGT
                     PLVYASTSRALACLETVVHLGGGIALPLNRYLVRIDIPAAAWAARTVFDGDQNVGWDA
                     LPSGRVSIAWGTAWAAQGDSLLAQVPSVIVPEEHNLLVNPAHPHAAQLRVTKLRRWLY
                     DPRALRLPA"
     gene            complement(3044..3541)
                     /locus_tag="PKV30_08280"
     CDS             complement(3044..3541)
                     /locus_tag="PKV30_08280"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRL30245.1"
                     /translation="MVASLSSSAAPKVSDRLGSYHLHGPATAAYWLSPMERSQRVKQG
                     VPAREVVTLADAMGLPRDQFMRVLGLARSTVERKITQRGPLSQAESEKLVGVERLIGQ
                     VDAMVREAGAAPDDFDAARWFARWMADPVPALGGVAPQELLDTADGREAVSTLLYQMK
                     SGVYA"
     gene            complement(3719..4918)
                     /locus_tag="PKV30_08285"
     CDS             complement(3719..4918)
                     /locus_tag="PKV30_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRL30246.1"
                     /translation="MTTTQQLLIAGGGIGGLAAALAVARAGWEVRLYERAAEFTEVGA
                     GVQLGPNVTRILQAWGLEEELRAVAAFPDRLQVRNATSGADLGTLALGARALQRYGAP
                     YATIHRADLHRILCMAAGSAPNVHLNLDHWLAGYTDDGKVVTLNTVAGQQIEGDALLG
                     ADGVWSRVRQQLLNDGPPRVTGHLAYRTMLCQSALPAALRSTQVTAWLGPRLHVVQYP
                     VRGGEWMNVVAIVHGDPPADAQGWDHRANAADLARAMGATCAPLRELIAAVTDATVND
                     HAWRLWALADRPPVEGAEHMACGLVALAGDAAHPMRPYLAQGAGMAIEDAAELARALA
                     MDAVDVPTRLKRYALARWQRCARVQARSIRNGQIFHSTGLMGLGRDASIKLLGERILD
                     VPWLYAG"
     gene            complement(5047..>5654)
                     /locus_tag="PKV30_08290"
     CDS             complement(5047..>5654)
                     /locus_tag="PKV30_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HRL30247.1"
                     /translation="AFLTGFTLSLSLIVAIGAQNAFVLRQGLRREHVGAVVAVCGLLD
                     VALMAAGVFGLGALVQSSPRALTVIAWAGAAVLAVYGLQALKRALAPGQLLASQAGQG
                     APRAQVVRQVLAISLLNPHVYLDTVVLVGAVGAGQPAALRPVFLAGAGAASALWFAAL
                     GYGARLLTPLFARPAAWRVLDLAVAALMFGIAWQLIASRLQ"
BASE COUNT          872 a         1970 c         1898 g          914 t
ORIGIN      
        1 gcgtgttcag caccgtctgc gtctgcgact tcaccttgtc cagcatgccg ccgaacttgc
       61 cgaactccgt cttcaccgcg cccagcactt gccacacctc gctgctgcgc ttttccagcg
      121 ccagcgttct gaaacccatc tgcaggctgt tgagcatggc cagcagcgtg gtcggcccgg
      181 ccagcgtcac gcggtggtcg cgctgcagcg cttccatcag gccggggcgg cgcagcactt
      241 cggcgtacag cccctcggtc ggcaagaaca agatcgcgaa gtcggtggtg tgcggcggct
      301 cgacgtactt gtcggccatg ccgcgcgctt cgagccggat gcgcgcctcc aaccctttgg
      361 ccgctgcctc ggcgcccacg gcgtcggcgc gctgctgcgc gtccagcagg cgttcgtagt
      421 cttcattggg aaacttcgcg tcgataggca gccagacagc gctggccgct cctgaatccg
      481 cagcaccgcg gcccggcagc ttgatggcga aatccaccgc gttgcggctg cccggccgcg
      541 tgcagacttg ggccgcgtac tgctcgggcg cgaacacctg ctccagcagc gcgcccagtt
      601 gcgcctcgcc gaacatgccg cgcgtcttga cgttggtcaa caggtgcttc aaatcgccga
      661 cgccctgcgc cagcgtctgc atctcgccca gccccttgtg cacctgctcc agccggtcgg
      721 ccacctgctt gaacgattcg cccaggcgcg cctgcagggt ggattgcaat ttttcgtcca
      781 ccgtctggcg catctcgtcg agtttggcgg tgttggcggc ctgcagctgg gccagctgct
      841 gctccagcgt gccgcgcacc tcggtcatgc ggcgcgcgtt ggtctcgctc aactgctgca
      901 actgctgcgt gagcgtctcc gacagcgagc cgcgcaggtg cgcgagctgc tgcgcaaagg
      961 cgtcgatctg cgcgttttgc gtgcgcgtgg cctcggccgc ctgctgcgcc atgccaaggc
     1021 ccagcgcctg caactgctgc accagggtgt cgcccagcgt gccgcgcaac tgtgtcagct
     1081 gcgcgcccag cgcgtcgacc tgcgcgttct gggtgcgcag tccctcggcg ccttggcgca
     1141 ccatcgcgtc ctgaaacgtc gccagcgcgt gcgacagctc ctgccgccct tcgcgcgcgc
     1201 tgacgccaat ctcttgtcgc agctcacgct ccagccgctc gccgtgctgg ccgacggccg
     1261 cgccgatcag ctgctgcacc tccccgcgcg aggccacgtc gtcgggtcgg cgcgggcgct
     1321 tcaacaacag caccagcaca agcagcaggt tcagggccag cgcggccagg gccagccaga
     1381 aaacccagtc gggccacgaa gaaccgttca tattactcct gattcaatag cttcctgcgc
     1441 tttatggatg ggcgctggct gcatttttta taaattaaat cagcgccgtg cgaacacttc
     1501 aggattcagt ggcgtcactg cctcgccctt tgtcaagtag ccgatcaggt tgtccgccgc
     1561 cagattcgcc atggcttggc gcgtgggtac ggtggcgctg ccgatgtggg gggtgagcac
     1621 gacgttggtg caggccagca ggtcggggtg gactttgggt tcgccctcga acacatccag
     1681 ccccgctgcg gcgatgcggc cttcgcgcag ggccttggcc aacgccacgt cgtcgacgat
     1741 gccgccgcgc gcgatgttga ccagcacggc tgtgggcttc atcagcgcca gttcggcggc
     1801 gccgatggtg tggtgcgatt ccttcgaata cggcacgacc agcaccacat ggtccgcggt
     1861 tttgagcaac tcttctttgc tgacgtagcg ggctttgact tcggcctcgt cactttcagg
     1921 caggcgcgag cggttgtgat agatcacctt catgccaaag cccagcgccc cgcgccgcgc
     1981 aatggcctgc ccgatgcggc ccatgccgat gatgccgagc gtggcgccgt gtacgtcttg
     2041 accgatgagc atgtcgtagg cccagcggtc ccactttcca tcgcgcaaaa agcgttcgcc
     2101 ctcggtcacg cggcgggcgg cggccatgag cagggcaaag cccatgtcgg cggtggtttc
     2161 ggtcagcacg ccgggcgcgt tggtggccag cacgccggca gcggtcatgg cgggcacgtc
     2221 gaagttgttg tagccgacgg ccatgttggc gcagattttc aggccggggc aggcttgcag
     2281 cacctcggcg tcgatgcgtt cgctgccggt ggtgaacacg ccttgctttc cgcctaagcg
     2341 gcggatgaat tcatccttga gccagatgtc gtcggcctga ttggcttcga cgtcgaagtg
     2401 cagggccagc ttatcgatga cttcagggaa gacggcgcgg gagacgagga cttgggggcg
     2461 tgcggcggtg ttcatgcggc cattctaggg atggccttgc gctcacgccg gcaggcgcag
     2521 cgcgcgcggg tcgtacagcc agcggcgcag cttggtcacg cgcaattgcg cggcgtgcgg
     2581 gtgcgcgggg ttgacgagca ggttgtgttc ttcgggcacg atgaccgagg gcacctgcgc
     2641 cagcaggctg tcgccctgcg cggcccaggc ggtgccccag gcgatgctga cgcgcccgga
     2701 gggcaaggcg tcccaaccga cgttttgatc accatcgaac acggtgcgcg cggcccacgc
     2761 ggctgcgggg atgtcgatgc gtaccagata gcggttgagc ggcaacgcga tgccgccgcc
     2821 taagtgcacc acggtttcaa ggcaggccag cgcgcgcgag gtgctggcgt agaccagcgg
     2881 cgtgccgcgc gcgttccagc ggccgccagt ggcgcgcgca ccggcgccgc tcaggtcgtc
     2941 tgcggtgtag tcgggcgtgt cggtggcgat gcgccagacg ctgatggcgc ctgtttcggt
     3001 ctcggcagtc actatgattt cgagagcttc tcgcgcttgt ccatcaagcg tacacgcccg
     3061 atttcatttg atacagcagc gtgctcacgg cttcgcggcc gtcggcggta tcgagcagct
     3121 cttgcggcgc cacgccgccc agcgccggca ccgggtcggc catccagcgg gcgaaccagc
     3181 gggcggcgtc gaaatcgtcg ggcgcggcgc cggcttcgcg caccatggcg tcgacctggc
     3241 caatcagacg ctcgacgccg accagctttt cgctctcggc ctgcgacagc gggccgcgct
     3301 gggtgatctt gcgctcgacc gtggagcgcg ccagccccag cacccgcatg aactggtcgc
     3361 gcggcaggcc catggcatcg gccagcgtga cgacctcgcg cgccggcacg ccttgcttga
     3421 cgcgctgcga acgctccatg ggcgacagcc agtacgccgc cgtggcgggg ccgtgcaggt
     3481 ggtagctgcc caggcggtca gacaccttgg gcgcggcaga ggaagaaagg gatgcgacca
     3541 tgaccatctc catcaatgga gataaataat atacccaatt gagacaaatg acaagcgcgc
     3601 ggttcgatga cgaaatgatt tcgctgcgct tcaatgacgg aatgaccgag gggcagtcat
     3661 ggcgcgcagc gccgtcatca atggcgtcag ccgaggtcat gcgccgccag gcgcgtcatc
     3721 accccgcgta cagccacggc acatccagaa tccgctcgcc cagcagcttg atcgacgcgt
     3781 cgcgccccag gcccatcagg cccgtggaat gaaagatctg cccattgcgg atcgagcgcg
     3841 cctgcacgcg agcgcagcgc tgccagcgcg ccagcgcgta gcgcttgagg cgcgtgggca
     3901 cgtcgaccgc gtccatggcc agcgcgcggg ccagttcggc cgcgtcttcg atggccatgc
     3961 cggcgccctg cgccaggtac ggccgcatgg ggtgcgcggc atcgcccgcc agcgcgacca
     4021 ggccgcacgc catatgctcg gcgccctcca ccggtggccg gtcggccagc gcccacaggc
     4081 gccaggcgtg gtcgttgacg gtggcatcgg tcacggcggc gatcagctcg cgcagcggcg
     4141 cgcaggtggc gcccatggcg cgcgccagat cggctgcgtt ggcgcggtgg tcccagcctt
     4201 gcgcgtcggc cggcggatcg ccgtgcacaa tggcgaccac gttcatccac tcgccgccgc
     4261 gcaccgggta ttgcacgacg tgcaggcgcg ggcccagcca ggcggtgact tgcgtgctgc
     4321 gcagcgccgc gggcagcgcc gactggcaca gcatggtgcg gtaggccagg tgcccggtca
     4381 cacgcggcgg gccgtcgttc agcaactgct ggcgcacgcg gctccacacg ccgtcggcgc
     4441 ccagcagcgc gtcgccctcg atctgctggc cggccacggt attcagcgtg accaccttgc
     4501 cgtcgtcggt gtagccggcc agccagtggt caaggttgag gtgcacattc ggcgcgctgc
     4561 ccgccgccat gcacaggata cggtgcagat cggcgcggtg gatggtggcg tagggggcgc
     4621 catagcgctg cagcgcgcgc gcgcccagcg ccaaggtgcc caggtccgcg ccgctggtgg
     4681 cgttgcgcac ctgcagccga tcagggaaag cagccacggc gcgcagttct tcctccaaac
     4741 cccaggcctg caggatgcgc gtcacgttgg ggccgagttg cacgcctgcc cccacttcgg
     4801 tgaactccgc cgcgcgttcg tacaggcgca cctcccaccc cgcgcgcgcc accgccagcg
     4861 ccgccgccag gccgccgatg ccaccgccgg caatcagaag ctgttgtgtt gtcgtcatgc
     4921 gccgattgtg ccgccgtgcg cggcgcggtc ggatgacgca gcgcaagcgt gtgcgacgcc
     4981 attcgattca agcaaaaagt gccgccagcg cttgtgggaa aagcgcgagc agctatggtt
     5041 tttgcattat tgaagccgtg acgcgatcaa ctgccaggcg atgccgaaca tcagcgccgc
     5101 caccgccaga tcgagcacgc gccacgctgc cggccgcgca aacagcggcg tgagcagccg
     5161 cgcgccatac cccagcgccg caaaccacaa cgcgctggcc gcacccgctc cggccagaaa
     5221 aaccggccgc agcgccgccg gctggcccgc acccacggcg ccgaccagca ccacggtgtc
     5281 cagatagacg tgcgggttga gcaggctgat cgccagcact tgccgcacca cctgcgcgcg
     5341 gggcgcgccc tgccccgctt gcgatgccag caactgtccc ggcgccagag cgcgcttcag
     5401 tgcctgcagg ccatacaccg ccagcaccgc cgcgccggcc caggcgatga cggtgagcgc
     5461 gcgcggcgat gattgcacca gcgcgcccag accgaacacg cccgccgcca tcagcgccac
     5521 atccagcagc ccacacacgg ccaccaccgc gcccacatgc tcgcgccgca agccctggcg
     5581 cagcacgaag gcgttctgcg cgccgatggc gacgatgagg gagaggctga gggtgaagcc
     5641 ggtgaggaag gcgg
//
LOCUS       DAOMVO010000152         5647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3928_length_5647_cov_15.345672, whole genome shotgun sequence.
ACCESSION   DAOMVO010000152 DAOMVO010000000
VERSION     DAOMVO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3928_length_5647_cov_15.345672"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKV30_08295"
     CDS             complement(<1..683)
                     /locus_tag="PKV30_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL30248.1"
                     /translation="MIDTWIAELPHGMRLSCRSAGERGRPVLMFLHGFPEAAFAWDEL
                     LAHFAAPEHGGYRCVAPNLRGYEQSSRPADVSAYHAKHLAQDIAALIAAECGPQGQLV
                     ALIAHDWGGAVAWNLAATRPELMQRLVIINSPHPQTFWRELRQNPAQQAASAYMNFLV
                     RPDAEALLAENDFARLWPFFDLMGASGAAAQQQAATALSDNAIGPGDAAQALALPDGA
                     GWMTDALRA"
     gene            891..1301
                     /locus_tag="PKV30_08300"
     CDS             891..1301
                     /locus_tag="PKV30_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRL30249.1"
                     /translation="MGIRIVRLGSPRAADEGVRIGTVRRPPRGVAKARFAADDWYDVW
                     YPNLSPTPDVMKLALSTHSEPDAAKADKDWALFTRLFRKEMAEPDAARTLDVLAALSQ
                     QTNFSVGCYCADEARCHRSILRALLGERGATLIA"
     gene            complement(1344..2051)
                     /gene="yihA"
                     /locus_tag="PKV30_08305"
     CDS             complement(1344..2051)
                     /gene="yihA"
                     /locus_tag="PKV30_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRL30250.1"
                     /translation="MPDSSPRPPRPNATAPASDAGRAALGWLHTARFLTTAAQLNQLP
                     ALDVPEIAFVGRSNAGKSTAINTLTQQNQLAFASKKPGRTQHINLFALGRQGSTDAVL
                     ADLPGYGYAAVPLADKQRWQRVMANYLVTRENLSGVVMLCDPRHGIKELDEALLEVIR
                     PRVEQGLPFLILLTKADKLNRTEQAKALQIAKLQAGGGDVILFSALKRQGVEDAALWL
                     RKVVEDATPEAESPASA"
     gene            2166..2783
                     /locus_tag="PKV30_08310"
     CDS             2166..2783
                     /locus_tag="PKV30_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL30251.1"
                     /translation="MKSLAALLTASLLSVHAFAADAPAAPKVDLAKGAASYAAVCVAC
                     HAADGNSTVPLQPSLAQQHPEYTFKQLLEFKSGARKDPIMQGFAATLSEADARNIAWW
                     LASQKAKPGFAKEKDLVMTGERIFRGGIPDRHIPACAGCHSPNGAGIPAQYPRLSGQH
                     ADYTVKQMVAFRDGTRANNAVMQGVAAKMLDREIKAVSDYIAGLR"
     gene            3052..5112
                     /locus_tag="PKV30_08315"
     CDS             3052..5112
                     /locus_tag="PKV30_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRL30252.1"
                     /translation="MRFAIALLTVICIASVIGTVIKQHEPAVNYTNQFGPFWAELFLA
                     VKLNAVYSAWWFLLILAFLVISTSLCIARNAPKFIADMRTYKENIREGSLKAFHHKAQ
                     GDLPQAPAAEAQRIGQLLATGGWKVRLQERDTPAGKGWMVAAKAGTANKIGYIAAHSA
                     IVLICLGGLFDGDLFVRALTWVGGKTVYSGGGLVSDVKPEHRLPASNPTFRGNLVVSE
                     GTASSTAILAQSDGILLQELPFAVELKKFIVEYYSTGMPKLFASDIVIHDLETGAKQD
                     ARVEVNHPASFKGINIYQSSFDDGGSSVKLRAVPMNGHAQPFEIDGTIGGASQITNGD
                     DKLTLELTALRVINVENLSDDRPGAASGADVRKVDLGHALTQRLGAADKTVTQRQLRN
                     VGPSITYKLRDAAGQAREFHNYMLPVDTGDGAAVFLLGVRNTPAEQFRYLRVPADDEG
                     NIDGFVRLRAALDDPAQRERAVRRYVAKAVDPARPELGDQLAASAARALGLFAGAEEV
                     KGQRASGLQAISDFMEAAVPEAERERAAEVLLRILNGSLFELNALAREGAGLKPLPES
                     EATQRYMTQAVLALSDAPLYPAPMAFELKDFKQVQASVFQVARAPGQNVVYLGCLLLI
                     LGVFAMLYIRERRLWVWLAPAGADGSRATMALSSNRKMMDTDREFDHLRQKLLHEQPE
                     GAKA"
     gene            5109..>5647
                     /locus_tag="PKV30_08320"
     CDS             5109..>5647
                     /locus_tag="PKV30_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HRL30253.1"
                     /translation="MNTTTAPANQGATLTLGDSFLARRTWGDWLFALLVVVGAVFAFQ
                     KYHAAMDVYEKGILIGTVPVMIWLGWFWRPLRTLMLVVAALSLLAIWLYGGGDLRRAD
                     SVFLLKYFLSSQSAILWMSMLFFMSTAFYWGGMAIRGQQDALEGLGSKIAWVAVGLAL
                     IGTMVRWYESHQIGPDIGH"
BASE COUNT          916 a         1873 c         1933 g          925 t
ORIGIN      
        1 tgcgcgcgca gggcgtcggt catccagccg gcgccgtcgg gcagcgcaag ggcctgggcg
       61 gcgtcgccgg ggccgatggc gttgtcggac agcgcggtgg cggcctgttg ctgcgcagcc
      121 gcgccgctgg cgcccatcag gtcgaagaag ggccacaggc gggcaaagtc gttttcggcc
      181 agcagcgcct cggcgtcggg gcggaccaag aagttcatgt acgcgctggc ggcctgctgc
      241 gcggggtttt gccgcaactc gcgccagaag gtctgcgggt gcggcgagtt gatgatgacc
      301 agccgctgca tcaactcagg ccgcgtggcg gccaggttcc aggccacggc gccgccccag
      361 tcgtgcgcga tgagtgcgac gagctggccc tgcggcccgc attcggcggc gatcagcgcg
      421 gcgatatcct gcgccaggtg cttggcgtgg taggccgaca cgtcggcggg gcggctggat
      481 tgctcgtagc cgcgcaggtt gggcgccacg cagcggtagc cgccgtgttc gggcgctgca
      541 aaatgtgcaa gcaactcgtc ccacgcgaag gcggcttcgg gaaaaccgtg caggaacatc
      601 agcaccggcc gcccgcgctc gccggccgag cggcagctca agcgcatgcc gtgcggcagc
      661 tcggcgatcc aggtgtcgat catgattgct ccttgatttg tagctgctgg cgcttgatgg
      721 acaagcgcca gtggcctgtt tgattcaaat aatgggcgat gcggcgacat cgtaggtgcc
      781 gcgccgggcg cgcgccgtct cgcgggtgac gatggcatcg ggtattctgg gtcaaaaatg
      841 cctccagcgc ttgtctatca agcgtcacca gctcctgaaa cgatagtgcc atgggcattc
      901 gcatcgttcg actgggctcg ccgcgagcgg cggacgaggg ggtgcgcatt ggcaccgtgc
      961 ggcgcccgcc gcgcggcgtg gccaaggcgc gcttcgccgc cgacgactgg tacgacgtgt
     1021 ggtaccccaa cctgtcgccg acgcccgatg tgatgaagct ggcgctgtcg acccacagcg
     1081 agccggatgc cgccaaggca gacaaggact gggcgttgtt cacgcgcctg ttccgcaagg
     1141 agatggcaga gcccgacgcg gcacgcacgc tggatgtgct ggcggcgctg tcgcagcaga
     1201 cgaatttttc ggtcggctgc tattgcgccg acgaagcgcg ctgccaccgc tccatcctgc
     1261 gcgcgctgct gggcgagcgc ggcgcgacat tgatcgcctg aagcgctggc cctatcaaaa
     1321 aatgagctgc ccgcgcttgc ccatcaagcg ctggcgggcg attcggcttc aggcgtggcg
     1381 tcttccacga ccttgcgcag ccacagcgcc gcgtcttcca cgccctggcg cttgagggcc
     1441 gaaaacagaa tcacgtcgcc gccgccggcc tgcagcttgg cgatctgcag cgccttggcc
     1501 tgctcggtgc ggttgagctt gtcggccttg gtcagcagga tcaagaacgg caagccttgc
     1561 tcgacgcgcg ggcggatgac ttcgagcagc gcctcgtcca gctccttgat gccgtggcgc
     1621 gggtcgcaca gcatgaccac gcccgacagg ttctcgcgcg tgacgaggta gttggccatc
     1681 acgcgctgcc agcgctgctt gtcggccagc ggcacggccg cgtagccgta gccgggcaaa
     1741 tcggccagca cggcgtcggt gctgccctgg cggccgagcg cgaacaggtt gatgtgctgc
     1801 gtgcggccgg gctttttcga cgcaaaggcc agctggttct gctgcgtcag cgtgttgatg
     1861 gcggttgact tgccggcgtt ggagcggccg acgaaggcga tctccggcac gtcgagcgcc
     1921 ggcaactggt tgagctgcgc ggcggtggtc agaaagcgcg cggtgtgcag ccagcccagc
     1981 gcggcgcgcc cggcgtcgga ggcgggtgcg gtggcgttcg ggcggggcgg gcgagggctg
     2041 gagtcgggca tgtcggcggt ggcccggccg gatgtgcggg aatcgcgtgg gagcacgggt
     2101 gggccattgt agaatcgtga ggttcagcgc cccacgattc ccaactctcc ataagacgtc
     2161 tgcccatgaa gtcgcttgcc gccctcctga ccgcatcgct gctcagcgtg cacgcctttg
     2221 ccgccgatgc gccggcagcc cccaaggtcg atctggccaa gggcgcggcg agctacgccg
     2281 ccgtgtgcgt ggcctgccat gcggccgacg gcaactccac ggtgccgctg caacccagcc
     2341 tggcccagca gcaccccgag tacaccttca agcaactgct cgagttcaag agcggcgccc
     2401 gcaaggatcc gatcatgcaa ggctttgccg ccacgctgag cgaagccgac gcgcgcaaca
     2461 tcgcgtggtg gctggcgtcg caaaaggcca agcccggttt cgccaaggag aaagacctgg
     2521 tgatgaccgg cgagcgcatc ttccgcggcg gcattcccga tcgccacatt cccgcctgcg
     2581 cgggctgcca cagccccaac ggcgccggca ttccggcgca atatccgcgc ctgtcgggcc
     2641 agcatgccga ctacacggtc aagcagatgg tggccttccg cgacggaacg cgcgccaaca
     2701 acgccgtgat gcagggcgtg gccgccaaga tgctcgaccg tgagatcaag gcggtgtccg
     2761 actacattgc cggcctgcgc tgagcgcgga ccgggcttcg tcgcctcgtc agccgcgccc
     2821 ggcttgccgg cgcggctttt gttttttggg caccggacgt gacccaggtc aagaaaaaag
     2881 acgatccagt gaacctgcac ccgacgcgcg cgctccaatg acgttcgcct ccatgcgccc
     2941 aggcgccccg atgtttcaag accgcgcatg accgccaccg acagcaccca aggttacgcc
     3001 ccccgacgcg gctctgaagc ctggcgccgc agcgtcgagc tgctgtcgtc gatgcgcttt
     3061 gccatcgcgc tgctcaccgt catttgcatc gcctcggtca tcggcaccgt catcaagcag
     3121 cacgaaccgg cggtcaacta caccaaccag ttcgggccgt tctgggccga gctgtttctg
     3181 gccgtcaagc tcaacgcggt ctacagcgcc tggtggtttt tgctgatcct ggcttttctg
     3241 gtcatcagca cgtcgctgtg catcgcgcgc aatgcgccca agttcatcgc cgacatgcgc
     3301 acctacaagg agaacatccg cgaaggcagc ctgaaggcgt tccaccacaa ggcccagggc
     3361 gatttgccgc aggcaccggc cgccgaggca cagcgcatcg gccagctgct ggccaccggc
     3421 ggctggaagg tgcgcctgca agagcgcgac acgcccgccg gcaagggctg gatggtcgcc
     3481 gccaaggccg gcaccgccaa caagatcggc tacatcgccg cgcacagcgc catcgtgctg
     3541 atctgcctgg gcggcctgtt cgatggcgac ctgttcgtgc gcgcgctgac ctgggtcggc
     3601 ggcaagacgg tgtattcggg cggcggcctg gtgtccgacg tcaagccaga gcaccgactg
     3661 ccggccagca accccacttt tcgcggcaac ctggtggtgt cggaaggcac ggcctccagc
     3721 accgccatcc tggcgcagtc cgacggcatc ctgctgcaag agctgccctt cgcggtcgag
     3781 ctgaagaagt tcatcgtcga gtactactcc accggcatgc ccaagttgtt cgccagcgat
     3841 atcgtgatcc acgatctgga aaccggcgcc aagcaggatg cgcgcgtcga ggtcaaccac
     3901 ccggcctcgt tcaagggcat caacatctac cagagcagct ttgacgacgg tggctccagc
     3961 gtcaagctgc gcgcggtacc gatgaacggc cacgcccagc cgttcgagat cgacggcacc
     4021 atcggcggcg cgtcgcaaat caccaacggc gacgacaagc tcacgctcga attgaccgcg
     4081 ctgcgcgtca tcaacgtcga gaacctgagc gacgaccggc cgggcgccgc atcgggcgcc
     4141 gacgtgcgca aggtcgatct gggccacgcg ctcacgcagc ggctgggcgc ggccgacaag
     4201 acggtgacgc agcgccagtt gcgcaacgtc ggccccagca tcacctacaa gctgcgcgac
     4261 gcggcgggcc aggcgcgcga attccacaac tacatgttgc ccgtggacac cggcgacggc
     4321 gccgcggtct tcctgctggg cgtgcgcaac acgccggccg agcaattccg ctacctgcgc
     4381 gtgccggcgg acgatgaagg caacatcgac ggctttgtgc ggctgcgcgc ggcgctggac
     4441 gacccggccc aacgcgagcg cgcggtgcgc cgctatgtgg ccaaggccgt cgatccggcg
     4501 cggccggagc tgggtgatca actggccgcg tcggccgcgc gtgcgctggg tctgtttgcc
     4561 ggcgccgaag aggtcaaggg ccagcgcgcg tcgggcctgc aggcgatctc cgacttcatg
     4621 gaagccgccg tgcccgaagc cgaacgcgag cgcgccgccg aggtgctgct gcgcatcttg
     4681 aacggcagct tgttcgagct gaacgcgctg gcacgcgaag gcgcgggcct gaagcccttg
     4741 cctgaaagcg aagccaccca gcgctacatg acgcaggccg tgctggcgct gtcggatgcg
     4801 ccgctgtacc cggcgccgat ggccttcgag ctgaaggact tcaagcaggt gcaagccagc
     4861 gtgttccagg tcgcgcgtgc acccggccag aatgtggtct atctgggctg cctgttgctg
     4921 atcctgggtg tttttgcgat gctgtacatc cgcgagcggc gcctgtgggt gtggctggcg
     4981 ccggccggcg ccgatggcag ccgcgccacc atggcgctgt cgtccaaccg caaaatgatg
     5041 gacaccgacc gcgagttcga ccacctgcgc cagaaactgc tgcacgagca gcctgaagga
     5101 gccaaggcat gaacaccacc actgcccccg ccaaccaagg cgccacgctg acgctgggcg
     5161 acagttttct cgcgcgccgc acttggggcg actggctgtt cgcgctgctg gtggtggtgg
     5221 gcgcggtgtt cgcgttccag aagtaccacg cggcgatgga tgtgtacgaa aagggcatcc
     5281 tgatcggcac cgtgcccgta atgatctggc tcggctggtt ctggcgaccg ctgcgcacgc
     5341 tgatgctggt ggtggcggcg ctgtcgctgc tggcgatctg gctgtacggc ggcggcgatc
     5401 tgcggcgcgc cgactcggtg tttttgctca agtacttttt gtccagccag tcggccatcc
     5461 tctggatgag catgctgttc ttcatgagca cggccttcta ctggggcggc atggccatcc
     5521 gcggccagca ggatgcgctg gaaggtctgg gcagcaagat tgcctgggtg gcggtgggcc
     5581 tggcgctcat cggcaccatg gtgcgctggt acgaaagcca ccagatcggc cccgacattg
     5641 gccacat
//
LOCUS       DAOMVO010000153         5639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3942_length_5639_cov_15.797994, whole genome shotgun sequence.
ACCESSION   DAOMVO010000153 DAOMVO010000000
VERSION     DAOMVO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_5639_cov_15.797994"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..927
                     /gene="xrtQ"
                     /locus_tag="PKV30_08325"
     CDS             22..927
                     /gene="xrtQ"
                     /locus_tag="PKV30_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase Q"
                     /protein_id="HRL30254.1"
                     /translation="MNTSLLTRSPRLLNTAMRIDRAPALLWIGLHTAALWPTWRWMAA
                     RVADGSDEPLGLLALAVLGALLWSVRRDLRCAPRLPWLALAVVGTVGATVLRSATSPL
                     VAALLAIAAWACGLLAFLPDVRRGAMRVAGGVLWPGRRHDIATLPVLGLAVLALPLIA
                     SLQFYAGYPLRVVTAEASRWLLSPWFAVLREGSSLNVNGLLVIVDAPCSGVQMAWAGY
                     FTACAVALLKGIGNRAFALRLPAVSVAVLAGNVVRNTLLVGLEGAGRAPGEALHQVIG
                     LLMLAAVCGAVGWVMSRPVRATQNY"
     gene            968..1528
                     /locus_tag="PKV30_08330"
     CDS             968..1528
                     /locus_tag="PKV30_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30255.1"
                     /translation="MKSFNTFHHRVLAKTVLAASALACALAGALPANDAAALARAAAE
                     MPATWQGEPVRPLALSAVEQRFAERFPGSVARLTDGRQIVVLRQVHQPTRMLHPAADC
                     YRGLGYRIEAERLERSTVAEDAPVQRCFTARKHGVALRVCEQIEDARGQRFADTSAWY
                     WAAVMGKSEGPWRAVTVTRPLTEGLM"
     gene            1610..3034
                     /locus_tag="PKV30_08335"
     CDS             1610..3034
                     /locus_tag="PKV30_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRL30256.1"
                     /translation="MTTVTTSLPPLLRRLVQLAVGVLLVIAIGAGALWAVLRIALAPA
                     AGEWATEIGRGPFALQASVPQLVWLATTPWIGERLHGVRVATRLGPVTLGWEPDSPSN
                     PAPALVLHCEPCTVPLPAGVGQPALTLPAAQLTLSRTLTAQNDQALDGLLLLGARALA
                     PDAEAPLLTAHWQARRAGPGWAVRLNWGEHPVRDWLALLAPQLPELARARIDGTLALS
                     ADLQLPERTLQLAPVLQGVSVQGLGTEAWAHLHSSCGPRVAVDARGWLARAVLAAEDQ
                     RFDEHPGYDLEELLTTLHTNQQRGAIARGGSTLTQQLAKLMVTGGERTLARKLREWLY
                     AVEMEQTLGKARILQLYLNLAPWGETAEGRLVCGAEAAARHHFNVPAQRLSPRQAITL
                     AAMLQNPTREAERWASEGSVDRERLVWIADQIRGVPVRQRRALAAQLRAEQAVVAAAS
                     IRSLSVAGTAPHASTVRLAGAAVR"
     gene            complement(3127..3594)
                     /locus_tag="PKV30_08340"
     CDS             complement(3127..3594)
                     /locus_tag="PKV30_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30257.1"
                     /translation="MSRCAPRTPRAVAALAWLLAAACHAQPGTAPMMAATAAALQVPP
                     ALQGRWTPVSKSLSGAGPLSLSAHTLTWSPCGPAPRAITAQNGPDGWLLTLPGQTGCR
                     LGGDPVTQLRAKPNPRNACEMELSVFSAALQTPPNERLAWGAYTRQGCAPAAR"
     gene            complement(3591..4013)
                     /locus_tag="PKV30_08345"
     CDS             complement(3591..4013)
                     /locus_tag="PKV30_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30258.1"
                     /translation="MKLLMNWALPIALGVMPVAAAAAAVTPAERAAIVASATQAAARE
                     LKLDAARLRLMPEQLKRQSDWAFLTAKLQTPAGQRFDYAGTDRHEAAQAGAVSDLCAA
                     LLRREGAAWKLVDIAVGPTDVAWEAWATTHKAPAELFR"
     gene            complement(4305..>5639)
                     /gene="safA"
                     /locus_tag="PKV30_08350"
     CDS             complement(4305..>5639)
                     /gene="safA"
                     /locus_tag="PKV30_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SafA/ExsA family spore coat assembly protein"
                     /protein_id="HRL30259.1"
                     /translation="RGDSLSAIASRHGVSLKSLIAANPQIANPQRIYPGDTITVPGGM
                     GGAAPGGAQPPASMRLSQDGLNMVKKYEGLFTKAYVCPAGVLTIGYGHTGPDVKPGQR
                     ITEAQAEQLLRQDMSKFEAAVKRQVKVPLTQGQFDALTSFTFNCGEGALKKSTLLKKL
                     NAGDYAGAQAEFQKWNKGGGRVLPGLVKRRAEEAQMFGSRAPQGGAAPGSPAPATPPS
                     GQASAPAASGSHTVKRGDTLWAIARANGVPLNELIAANPQIKNPNLIYPGQKVNIPVG
                     GNAPGPTGGTTGPSGPSTPTPAGNASGARTAQIAESFLNRNASELKRSGDLPMNPNVP
                     SNVCCANFVSAVLEKNGLLKKGEHTDSVSQLDATLRGKGWKSVSMANAKPGDVVIMKR
                     GGVSHTEIVAKNENGKITLVGSNNRNADGSQRITYDSSTWWHSKVSGILTPP"
BASE COUNT          734 a         1964 c         2042 g          899 t
ORIGIN      
        1 gcgcttaagg cccaaaccat catgaacacc tccttgctca cccgctcgcc ccgcctcctc
       61 aataccgcca tgcgcatcga tcgcgccccc gcgctgttgt ggatcggcct gcacaccgct
      121 gccctgtggc ccacctggcg ctggatggcg gcgcgcgtgg ccgacggatc ggacgagccg
      181 ctcggcctgc tcgcgctggc cgtgctgggc gcactgctgt ggtcggtgcg gcgcgatctg
      241 cgctgcgcgc cgcgcctgcc ctggctggcg ctggccgtgg tgggcacggt gggcgcgacg
      301 gtcctgcgtt cggcaacgtc gccgctggtg gcggcgctgc tggccattgc cgcctgggct
      361 tgcggcttgc tggcttttct gcccgacgtg cggcgcggcg ccatgcgcgt ggccggcggc
      421 gtgctgtggc cggggcggcg gcacgacatc gccacgctgc cggtgctggg gctggcggtg
      481 ctggcgctgc cgctcatcgc ctcgctgcag ttctacgccg gctacccgct gcgtgtggtc
      541 accgccgaag ccagccgctg gctgctgtcg ccgtggtttg ccgtgctgcg tgaaggcagc
      601 agcttgaacg tgaacggcct gctggtcatc gtcgatgcgc cgtgttcggg cgtgcagatg
      661 gcgtgggcgg gctacttcac ggcgtgcgcg gtggcgctgc tgaaaggaat cggaaaccgc
      721 gcgtttgcgc tgcggctgcc ggcggtcagc gtggcggtgc tggccggcaa cgtcgtgcgc
      781 aacacgctgc tggtggggct tgaaggcgcg ggccgcgcgc ccggcgaggc gctgcaccaa
      841 gtcatcgggc tgctgatgct tgcagcggtg tgcggcgccg tcggctgggt catgtcgcgc
      901 cccgtgcgcg cgacgcaaaa ctactaaatc aatagctgct tgcgcagtct ggacgggcga
      961 caacaccatg aaaagcttca acaccttcca tcaccgcgtg cttgccaaga ccgtgctggc
     1021 cgccagcgcg ttggcctgcg cactggccgg cgcgctgcct gccaacgacg ccgccgcgct
     1081 tgcgcgcgcc gccgccgaga tgccggccac ctggcaaggc gagccggtgc ggccgctggc
     1141 cttgtcggcg gtcgagcagc gctttgccga gcgctttccc ggcagcgtgg cgcgcctgac
     1201 cgacggccgg cagatcgtgg tgctgcgcca ggtgcaccag cccacgcgca tgctgcatcc
     1261 ggcggcggac tgctatcgcg ggctgggtta tcgaatcgag gctgaacgac tggaacgaag
     1321 cacggtggct gaagacgccc ccgtgcagcg ctgcttcacc gcccgcaagc acggcgtcgc
     1381 gctgcgcgtg tgcgagcaga tcgaagacgc gcgcggccag cgctttgccg acacctccgc
     1441 ctggtactgg gcggcggtga tggggaaaag cgaagggccg tggcgcgccg tcacggtcac
     1501 aaggccgctg accgaaggtc tgatgtgaaa agcggccccg gcgtttgaat agcaagcgca
     1561 ggcagctatc aaaaacatag aaatcaaccg cttcacccag gagttcgcca tgaccaccgt
     1621 cacgacttct ctcccgccac tgctgcgccg cttggtgcaa ctggctgtcg gcgtgctgct
     1681 ggtgatcgct atcggcgcgg gcgcgctgtg ggccgtgctg cgcatcgcgc tcgcgccggc
     1741 cgcgggcgaa tgggcgaccg aaatcgggcg cggccccttc gcgctgcagg ccagcgtgcc
     1801 gcaactggtg tggctggcca ccacgccgtg gatcggcgag cgcctgcacg gcgtgcgcgt
     1861 ggccacccgg ctgggccccg tcacgctggg ctgggagccg gacagcccga gcaaccccgc
     1921 gcccgcgctg gtgctgcatt gcgagccgtg caccgtgccc ctgcccgccg gcgtcggcca
     1981 gccagcgctg acgctgccgg ccgcgcagct cacgctgtcg cgcaccctca cggcgcagaa
     2041 cgatcaggcg cttgacggct tgctgctgct gggcgctcgc gcccttgcac ccgatgccga
     2101 agcaccgctg ctcaccgccc actggcaagc gcgccgcgcc ggccccggct gggccgtgcg
     2161 cttgaactgg ggcgagcacc cggtgcgcga ctggctggcc ttgctggcgc cgcagttgcc
     2221 cgaactggcc cgcgcgcgca tcgacggcac gctggccctc agcgccgatc tgcaactgcc
     2281 cgagcgcacg ctgcaactgg cgccggtgct gcaaggcgtg tcggtgcaag gcctgggcac
     2341 cgaggcctgg gcgcacctgc attcgtcgtg cggcccgcgc gtggcggtcg atgcacgcgg
     2401 ctggctggcg cgcgccgtgc tggcggccga agaccagcgc tttgacgaac accccggcta
     2461 cgacttggaa gagctattga ctacgctgca caccaaccag cagcgcggcg ccatcgcgcg
     2521 cggcggcagc accctcacgc agcagctcgc caagctgatg gtcaccggcg gcgagcgcac
     2581 gctggcgcgc aagctgcgcg aatggctgta cgcggtggag atggagcaga cgttgggcaa
     2641 ggcgcgcatc ctgcagctgt acctgaacct cgcaccgtgg ggcgaaaccg ccgagggccg
     2701 cctggtgtgc ggcgccgaag ccgccgctcg ccaccacttc aacgtgcccg cacagcgcct
     2761 gtcgccccgc caggccatca ccctggccgc catgctgcaa aaccccacac gcgaggccga
     2821 gcgctgggcc agcgaaggca gtgtcgaccg cgagcggctg gtgtggatcg ccgatcagat
     2881 tcgcggcgtg ccggtgcggc agcggcgcgc gctggcggcg caactgcggg ccgagcaagc
     2941 tgtggtggcg gccgcgtcca tcaggagttt gtctgtggcc ggcacggcac cgcatgcgtc
     3001 aaccgtgcgt ctggcgggcg cggcggtgcg ctgacagcgc cacggcgcgg cgattcgcac
     3061 cgaccccgcc gttgctgccg acgcgcagcg cgctctgcgc ctctgcggtg catgccttgt
     3121 gtggcttcag cgcgccgctg gggcgcagcc ctggcgcgtg tacgcacccc aggccagtcg
     3181 ctcgttgggt ggcgtctgca atgcggcgct gaagaccgac agctccatct cgcacgcgtt
     3241 gcgcgggttg ggcttggcgc gcagctgggt gaccgggtcg ccgcccagcc ggcagccggt
     3301 ctggccgggc agcgtcagca gccagccgtc ggggccgttt tgcgcggtga tggcgcgcgg
     3361 cgccgggccg cagggcgacc aggtcagggt gtgcgcgctc aggctcagcg ggccggcgcc
     3421 ggacaaggac ttgctgaccg gcgtccagcg gccctgcagc gcgggcggca cctgcagcgc
     3481 agcggccgtg gcggccatca tgggggcggt acccggctgc gcgtggcagg cagcggccag
     3541 cagccacgcc agtgcggcga cggcgcgggg ggtgcgcggc gcgcagcggc tcatcggaac
     3601 agctccgcgg gcgccttgtg cgtggtggcc caagcttccc atgccacatc ggtcgggccg
     3661 acggcaatgt cgaccagctt ccacgccgcg ccttcgcggc gcagcagcgc ggcgcacagg
     3721 tcggacacgg cgccggcctg cgcggcctca tgccggtcgg tgccggcgta gtcgaagcgc
     3781 tggccggcgg gggtttgcag cttggcggtc aagaaagccc agtcgctctg ccgcttgagc
     3841 tgctcgggca tcaggcgcag ccgcgcggca tcaagcttca gctcgcgcgc ggcggcctgc
     3901 gtggcgctgg cgacgatggc cgcgcgctcg gcgggggtga cggccgctgc ggcggcagcc
     3961 accggcatca cgcccagggc gatgggcagc gcccaattca tcagcaattt catgtcaaca
     4021 ccttgtggag tttgcgcacg tcgcattcaa ccatcgtccg gcgaagccgg tgcgacaaaa
     4081 cgtagtcgct gcatgcacta tcaaaaaacg agctgcccgc gctgaataga caagcgccgg
     4141 cagcagtttt attcagaaga agccgccctg aaaagcgact ctgaccgtcc gggctgcgca
     4201 gcaagcccgg ccacgaggcc gcggagcagg ccacgccgga acgccgcggc cggggtcccc
     4261 ccggccgcca gcgttgtccc cctgggggga aggcgcgcag cgcctcaggg gggggtcaat
     4321 atcccgctga ccttgctgtg ccaccaggtg ctgctgtcgt aggtgatgcg ctggctgccg
     4381 tcggcgttgc ggttgttgga gccgaccagg gtgattttgc cgttctcgtt tttggccacg
     4441 atctcggtgt gcgacacgcc gccgcgcttc atgatgacca cgtcgccggg cttggcgttg
     4501 gccatgctga ccgacttcca gcccttgccg cgcagcgtgg cgtcgagctg cgagaccgag
     4561 tcggtgtgct cgcccttctt gagcaggccg tttttctcca gcacggccga gacaaagttg
     4621 gcgcagcaca cgttgctggg cacgttcggg ttcatgggca gatcgccgct gcgcttcaac
     4681 tcggacgcgt tgcggttcag aaaactctct gcaatctgcg ccgtgcgcgc gccgctggcg
     4741 ttgccggcgg gcgtgggtgt gctcgggccg ctggggccgg tcgtgccgcc ggttggcccc
     4801 ggtgcgttgc cgccgacggg gatgttgacc ttctgccccg ggtagatcag gttggggttc
     4861 ttgatttgcg ggttggcggc gatcagctcg ttcagcggca cgccgtttgc gcgcgcaatg
     4921 gcccacagcg tgtcgccgcg cttcactgtg tggctgccgc tggcggcggg cgcactggcc
     4981 tggccgctgg gcggcgtggc gggcgctgga gatcccggcg ccgcgccacc ttgcggcgcc
     5041 cggctgccga acatctgcgc ttcttcggcg cgccgcttga ccagcccggg cagcacgcgg
     5101 ccgccgccct tgttccactt ctggaactcg gcctgcgcgc cggcgtagtc gcccgcgttc
     5161 agctttttca gcaaggtcga cttcttcagc gcgccttcgc cgcagttgaa ggtgaagctg
     5221 gtcagcgcat caaactgccc ctgcgtcagc ggcaccttga cctggcgctt gacggcggcc
     5281 tcgaacttgc tcatgtcctg gcgcagcagc tgctcggcct gcgcctcggt gatgcgctgg
     5341 ccgggcttga cgtccggccc ggtgtggcca taaccgatgg tcagcacgcc ggccgggcag
     5401 acatacgcct tggtgaacag gccctcgtac ttcttgacca tgttcagccc gtcctggctc
     5461 aggcgcatgc tggccggcgg ctgcgcgcca cccggcgccg cgccgcccat gccgcccggc
     5521 accgtgatgg tgtcgccggg gtagatgcgc tgcgggttgg cgatctgcgg gttggcggca
     5581 atcagcgact tcagcgacac gccgtggcgc gacgcaatgg ccgagagcga gtcgccgcg
//
LOCUS       DAOMVO010000154         5638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3944_length_5638_cov_14.141322, whole genome shotgun sequence.
ACCESSION   DAOMVO010000154 DAOMVO010000000
VERSION     DAOMVO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3944_length_5638_cov_14.141322"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKV30_08355"
     CDS             <1..479
                     /locus_tag="PKV30_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HRL30260.1"
                     /translation="KQRLFERIDAEIGPMRERYQHLIAHPEEVEQLLLRGAEKARAIA
                     TPFIRELRHAVGLRPLQAAAQAGQAAEATKTAATGSFKQYRETDGRFYFKLVDAAGQV
                     LLQSLGFDSPRDAGQAVRRLQTEGAAALPPLAAQLEQTGAPSPALLNALSTLQIAP"
     gene            588..1193
                     /locus_tag="PKV30_08360"
     CDS             588..1193
                     /locus_tag="PKV30_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL30261.1"
                     /translation="MAIYVIDDHPLMREAVVMLLRRVRPGSDVVELSQLSDLDRAVAT
                     KGAPELFCLDLKLPDTVGVSGVKLLKGLYPEIPLAVISATPAADVEEWCREAGADVYV
                     EKSAGASEIAAALRAMLLTDSQFGDLEDGEAKAETKLSKRQKQLIVMLDVGLSNREIA
                     ERLGISEHTVKVHLWRLFRRLGVKSRTQTLHFARMNGLLNS"
     gene            complement(1135..2577)
                     /locus_tag="PKV30_08365"
     CDS             complement(1135..2577)
                     /locus_tag="PKV30_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL30262.1"
                     /translation="MPRNPPASKAVDPIIRYSHADWAEAAMMASVMKGQRAALWPAVA
                     VVVVLAATCYPHVPRWELALMVVVSMVVTVVRLRFVQGYGRLAKTDRIDVQLSYLHRY
                     RGFWILNGVAWGMWPLLFYGRLPAAPTVACWMLTAGVGGVAIAWMSAHLRTTRAFLMS
                     FLLSLAISVGLAFAVRPHTLLQQADYWVPVLLGLYWLLLLRIVRHLHELYRSSIDLSY
                     HNARLIYSLQEQTRVAQEASRFKDRFLAGAAHDLKQPVSALSIYAEWLSSEPELVDEL
                     GPKILQSTRAINALFDSLFDLVKLDAGRLETDMQTLQVRALLDDLEVQFEPMATQKGL
                     QLRVRAIDAALVSDRIMLRRIVGNLVANAIRYTAQGGVLLTARPRGQAISFEVWDTGI
                     GIPDHEQSQIYEEFYKVPTSGTEEGFGLGLAIVRRLSERLGYTVSLRSRAGRGTVFRV
                     LVPPLPAPNPSLRNSAARSSGQSAGSASGS"
     gene            complement(2618..3715)
                     /gene="aroC"
                     /locus_tag="PKV30_08370"
     CDS             complement(2618..3715)
                     /gene="aroC"
                     /locus_tag="PKV30_08370"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRL30263.1"
                     /translation="MSGNTLGKLFAVTNFGESHGPAIGCVIDGCPPGLSLSEAVIQPE
                     LDRRRPGTSKFVTQRNEADQVEILSGVYQGQTTGTPICLLIRNTDQRSKDYGDIAQSF
                     RPGHADYTYWRKFGVRDPRGGGRSSARLTAPTVAAGAVARKWLHERHGTTFRGCMTHV
                     GELAIGFEDWAHVPNNPFFAPVADVSAIEDYMNALRKAGDSCGARLRVTAQHVPVGLG
                     EPIYDRLDADIAHAMMGLNAVKGVEIGDGFASVAHRGSMHGDSLRPGGAFESNHDGGV
                     LGGISSGQALEVALAIKPTSSILVPRESLNTAGETVEVITKGRHDPCVGIRATPIAEA
                     LLALVVMDHALRHRAQCADVAVPSGFGPRSA"
     gene            complement(3746..4201)
                     /locus_tag="PKV30_08375"
     CDS             complement(3746..4201)
                     /locus_tag="PKV30_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HRL30264.1"
                     /translation="MSWKTLVALRAHLDREGLAGGLRFLNERVPHRFTGVYRLVDGTM
                     HNVGLADKRGEIAPEALLAVPLTSSFCQFVLRDGQFASARTGQDARLDGHPYQGVLNT
                     YVGLPLTHTGEDLRGTLCHFDFDPVDVSQEELEFLRRAALLLPRYLPRG"
     gene            complement(4256..4723)
                     /locus_tag="PKV30_08380"
     CDS             complement(4256..4723)
                     /locus_tag="PKV30_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRL30265.1"
                     /translation="MKVRDILALKGNALYTITPEDSLADAAAVMAQKDIGSLVVMSHE
                     RVVGILTFREVIEATVRNGGSFGTTTVYRAMNAGPLICTLETELDEVRRMMVDHHARY
                     LPVIDKQMLQGVISFYDVAKTVVDRQDFENRMLKAYIKDWPEEDSKNTEPNFP"
     gene            4843..>5638
                     /locus_tag="PKV30_08385"
     CDS             4843..>5638
                     /locus_tag="PKV30_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL30266.1"
                     /translation="MPGHADPGFDTLSIHAGAQPDPATGARAVPIHLSTSFVFDSSDH
                     AVSLFNLERSGHVYSRISNPTNAVLEQRVAALEGGIGAIAVASGQAALHLAIATLMGA
                     GGHIVASTALYGGSQNLLHYTLRRFGIETTFVKPGDLDGWRAAIRPNTRLLFGETVGN
                     PGLEVLDIPAVGQIAHDAGLPLLVDSTFTTPYLLKPFDHGADLIYHSATKFLSGHGTV
                     IGGVLVDGGSFDWDKSGKFAELTEPYDGFHNMVFSEESTVGAFLLRA"
BASE COUNT          966 a         1902 c         1841 g          929 t
ORIGIN      
        1 ccaagcagcg gctgttcgag cgcatcgatg ccgaaatcgg ccccatgcgc gagcgctacc
       61 agcacctgat cgcgcaccca gaagaagtcg agcagttgct gctgcgcggc gccgaaaaag
      121 cccgcgccat cgccacgccc ttcatccgcg agctgcgcca cgccgtgggt ttgcggccat
      181 tgcaggctgc agcgcaagcg gggcaagcgg cagaagctac taaaaccgca gcaaccggca
      241 gcttcaagca gtaccgcgag accgacgggc gcttttactt caagctcgtc gacgcggccg
      301 gtcaggtgtt gctgcaaagc ctcggcttcg attcgccgcg cgacgcgggg caagccgtca
      361 ggcgcctgca aaccgagggc gcggcggcgc tgccacctct ggctgcgcag cttgagcaga
      421 caggcgcgcc ctcgccggcc ttgctgaatg cgctttctac gctacagatt gcgccgtgaa
      481 tgtgaagttt tcgggcttgt gagccgagcc ccgccgcccc gacggcgata cccgaccccg
      541 tgatgcacaa tggatcgtaa ttaatagaag ctctgggaga attgggtttg gccatttacg
      601 tcatcgacga tcaccccctc atgcgcgagg ccgtggtcat gctgttgcgg cgcgtgcggc
      661 cgggttccga cgtggtcgaa ctgtcgcagt tgtccgatct ggaccgtgcc gtggccacca
      721 aaggcgcgcc agagctgttc tgccttgacc tgaagctgcc cgacacggtg ggcgtctccg
      781 gcgtcaagct gctcaagggc ctgtaccccg agattccgct ggccgtgatc tccgccacgc
      841 cggctgccga cgtcgaggaa tggtgccgcg aagccggcgc cgacgtctac gtcgagaaat
      901 ccgccggcgc gagcgaaatc gccgccgccc tgcgcgccat gctgctcacc gacagccagt
      961 ttggcgacct ggaagacggc gaggccaagg ccgaaaccaa gctctccaag cgccaaaagc
     1021 agctgatcgt gatgctggac gtaggcctgt ccaaccgcga gatcgccgag cgcttaggta
     1081 tcagcgagca caccgtgaag gtgcacctgt ggcggctgtt ccggcgtctc ggcgtcaaga
     1141 gccggacgca gaccctgcac tttgcccgga tgaacgggct gctgaattcc tgagcgatgg
     1201 gtttggcgcc ggcagcggcg gcaccagcac cctgaacacg gtgccgcgcc cggcgcgcga
     1261 gcgcagcgac acggtgtagc ccagccgttc ggacagccgc cgcacgatcg ccaggcccag
     1321 accaaagcct tcctctgtgc cgctggtcgg caccttgtag aactcctcat agatctggga
     1381 ctgctcatga tcgggaatgc cgatgccggt gtcccagacc tcgaagctga tcgcctggcc
     1441 gcgcggccgc gccgtcagca gcactccgcc ctgcgccgtg tagcggatgg cgttggccac
     1501 caggttgccg acgatgcggc gcaacatgat gcggtcggat accaaagccg catcgatggc
     1561 gcgaacgcgc aattgcaagc ctttctgggt cgccatcggc tcgaactgca cctccagatc
     1621 gtccagcagc gcgcgcacct gcaaggtctg catgtcggtc tctaagcggc cggcatcgag
     1681 cttgaccagg tcgaacaacg agtcgaatag ggcgttgatg gcccgcgtcg actgcagaat
     1741 cttggggccc agttcgtcca ccagttcagg ctcgctgctc agccattcgg cgtagatgct
     1801 caaggcgctg accggctgct tgagatcatg cgccgcgccg gccagaaagc ggtccttgaa
     1861 gcgcgaggcc tcttgcgcca cgcgcgtctg ctcttgcagc gaatagatca gccgcgcgtt
     1921 gtggtagctc aggtcgatgc tgctgcggta cagctcgtgc aggtggcgca cgatgcgcaa
     1981 caacagcaac cagtacaggc cgagcagcac cggcacccag tagtcggcct gctgcaacag
     2041 ggtgtggggc cgaacggcga aagccaagcc cacgctgatc gccagcgaga gcaagaacga
     2101 catcagaaac gcccgcgtgg tccgcaggtg ggccgacatc caggcgatgg ccacgccacc
     2161 cacaccggcc gtcagcatcc agcaggccac ggtcggggcc gccggcagcc ggccatagaa
     2221 cagcagcggc cacatgcccc aggccacgcc gttcagtatc cagaagccgc ggtaacggtg
     2281 caggtagctc agttgaacgt cgatgcggtc ggtttttgcc aggcgtccgt agccttgcac
     2341 gaaccgcaaa cgcaccacgg tgaccaccat cgacaccacc accatgagcg ccagctccca
     2401 gcgcggcacg tgcgggtagc aagtggccgc cagcaccacc accaccgcga cggcgggcca
     2461 caaggcggca cgctggccct tcatcaccga ggccatcatg gcggcctcgg cccagtccgc
     2521 gtgcgagtag cggatgatcg ggtcgaccgc cttgctggcc ggtgggttgc gcggcatcgg
     2581 tctgccttgt gcggtcagcg gatgtggata acggccttca ggcgctgcgc gggccgaagc
     2641 cgctcggcac cgccacgtcg gcgcactgcg cgcggtggcg cagtgcgtgg tccatcacga
     2701 ccagggccag cagcgcttcg gcaatcggcg tggcgcggat gccgacgcag gggtcgtggc
     2761 ggcccttggt gatcacttcc acggtttcgc cggccgtgtt caaggattcg cgcggcacca
     2821 ggatcgagct ggtgggcttg atggccaggg ccacttccag agcctgcccg ctgctgatgc
     2881 cgcccagcac gccgccgtcg tggttgctct cgaaagcacc gcccggccgc agcgaatcgc
     2941 cgtgcatgct gccgcggtgc gcgacgctgg caaagccgtc gccaatttcc acgcccttca
     3001 ccgcgttcag gcccatcatg gcgtgcgcga tgtcggcgtc aaggcggtcg tagatcggct
     3061 cgccgaggcc cacgggcacg tgctgcgccg tcacgcgcag gcgcgcgccg cacgaatcgc
     3121 cggccttgcg cagcgcgttc atgtagtcct cgatggccga cacatcggcc acgggggcga
     3181 agaaggggtt gttcggcacg tgcgcccaat cctcgaagcc gatggccaac tcgcccacgt
     3241 gcgtcatgca gccgcgaaac gtggtgccat ggcgctcatg cagccacttg cgcgccacgg
     3301 cacccgccgc caccgtgggc gcggtcagcc gcgccgacga gcgcccgccg ccgcgcgggt
     3361 cgcgcacgcc gaatttgcgc cagtaggtgt agtcggcatg gccggggcga aagctttgcg
     3421 cgatgtcgcc atagtccttg ctgcgctggt cggtgttgcg gatcagcagg cagatcggcg
     3481 tgccggtggt ctggccttgg tacacgccgg acaggatctc gacctgatcg gcctcgttgc
     3541 gctgggtgac gaatttcgag gtaccggggc ggcgccgatc cagctcgggc tggatgaccg
     3601 cctcgctcaa gctgagcccc ggcggacagc cgtcaatgac gcagccaatg gccgggccgt
     3661 gggattcgcc gaaattggtg acggcaaaca gcttgccgag ggtgttgccg ctcatgcggc
     3721 cgattatcgc aacccgtgga tgggctcagc cgcgcggcag atagcgcggc aacaggagtg
     3781 cggcacggcg cagaaactcg agctcctcct gcgacacgtc gaccgggtcg aagtcgaagt
     3841 ggcacagcgt gccgcgcaga tcctcgcccg tgtgcgtgag cggcagtcca acgtaggtgt
     3901 tgagcacgcc ttggtagggg tggccgtcaa ggcgcgcatc ctgcccggtg cgggccgacg
     3961 cgaactgacc atcgcgcaac acaaactggc aaaaactgct ggtcagcggc acggccagca
     4021 aggcttcagg cgcgatttcg ccgcgcttgt cggccaggcc gacgttgtgc atggtgccgt
     4081 ccaccagccg atacacgcca gtgaatcggt gtggcacccg ctcgttcaaa aagcgcaggc
     4141 cgccggccag tccttcacgg tccagatggg cgcgcagcgc caccagtgtt ttccatgaca
     4201 aggattgtca cggatagtgc cgtcggcccc tgccgagctt gtttcgcccg ccgattcagg
     4261 ggaaattggg ctccgtgttc ttgctgtctt cctcaggcca gtccttgatg taggccttga
     4321 gcatgcggtt ctcgaaatcc tgccggtcga ccactgtctt ggcgacgtcg tagaagctga
     4381 tcacgccctg cagcatctgc ttgtcgatca ccggcaggta gcgtgcgtgg tggtcgacca
     4441 tcatgcggcg cacctcgtcg agttcggtct ccagcgtgca gatcagcggg ccggcgttca
     4501 tcgcgcggta caccgtggtg gtgccgaagc tgccgccgtt gcgcaccgtc gcctcgatga
     4561 cttcgcggaa cgtgagaatg cccaccacgc gctcgtgcga catcaccacc agcgagccga
     4621 tgtccttttg ggccatcacc gcggccgcgt cggccaggct gtcttcgggc gtgatggtgt
     4681 acagggcgtt gcccttgagg gccaggatgt cgcggacttt catggggctt gaggccagcg
     4741 cctgcggccg gcgcggcgcg gtgtgcggtt gacgcgcttg aatatagccc acaataaaac
     4801 catcgaacgg ccccgcccgg ggcgcacacc acaggagagc ccatgcccgg ccacgccgac
     4861 cccggtttcg acaccctcag catccacgcg ggcgcgcaac ccgaccccgc caccggcgcg
     4921 cgcgcggtgc cgatccatct gtccacctcg ttcgtgttcg attcgagcga tcacgccgtc
     4981 tcgctgttca atctggagcg ctcgggccac gtctacagcc gcatcagcaa cccaaccaac
     5041 gcggtgctgg agcagcgcgt ggcggcgctt gagggcggca tcggcgcgat cgctgtcgcc
     5101 agcggccaag ccgcgctgca cctggccatt gccacgctga tgggcgcggg cggccacatc
     5161 gtcgccagca cggcgctgta cggcggcagc cagaacctgc tgcactacac gctgcgccgt
     5221 ttcggcatcg agacaacctt tgtcaaaccc ggcgacctgg acggctggcg cgccgccatc
     5281 cggcccaaca cgcggctctt gtttggcgag acggtgggca acccggggct ggaggtgctc
     5341 gacatccctg ccgtcgggca aattgcgcac gacgccggct tgccgctgct ggtcgattcg
     5401 acctttacca cgccgtacct gctcaaaccc ttcgaccacg gcgctgacct gatttaccac
     5461 tcagccacca aattcctctc cggccacggc accgtgattg gcggcgtgct ggtcgatggc
     5521 gggagtttcg actgggataa gagtgggaaa ttcgccgagc tgaccgagcc ctacgacggc
     5581 ttccacaaca tggtgttcag cgaggaaagc accgtcggcg cctttttgct gcgcgcgc
//
LOCUS       DAOMVO010000155         5631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3953_length_5631_cov_14.734397, whole genome shotgun sequence.
ACCESSION   DAOMVO010000155 DAOMVO010000000
VERSION     DAOMVO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_5631_cov_14.734397"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKV30_08390"
     CDS             <1..314
                     /locus_tag="PKV30_08390"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520636.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRL30267.1"
                     /translation="DEFLKFFDGFSIGSNDLTQLTLGLDRDSGMELLAADFDERDPAV
                     KAMLQRAIQACKAQGKYVGICGQGPSDHPDFARWLADEGIASISLNPDSVIDTWTLLA
                     T"
     gene            339..602
                     /locus_tag="PKV30_08395"
     CDS             339..602
                     /locus_tag="PKV30_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRL30268.1"
                     /translation="MSNVPNTIKREAASAVWVRATLLFVWVLASFGMAFFARDLGQTV
                     AGWPLAFWLAAQGSVLVFIGIVVVYAWWMNRHEAATLAGREGD"
     gene            605..2656
                     /locus_tag="PKV30_08400"
     CDS             605..2656
                     /locus_tag="PKV30_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HRL30269.1"
                     /translation="MPGAPRADLWRLHRHLLLFVLGFIAFLLLMHSAERNGLSRDWIG
                     PIFLFLSIMLYACIGIYSRTTDPEDFYVAGRRIPAMYNGMATAADWMSAASFISLSGA
                     LYLQGFSGTAAQPGGLAYVLGWTGGFCLVGLLIAPRLRAMALYTVPDFYAVRFGGRWP
                     RLLAALGAVVCSFMYVVAQIYGIGLIASRLTGVQFEIGILLGLGGVLLCSFLGGMRAI
                     TWTQVVQYVVLLLAFLIPVSWLAYKQLGNPLAPLAYGAQLPKIAQMEERLINDPAERE
                     VRAAFAARAQEYAARLNDPATALAREHQRLNERIRTLKAQGADFSLVMAARRELAELP
                     RDVASARDLWGRAMSEAQTRSGPLGGLPRQGQAFAGDPNGTPDDQRAFQDSRLNFLAL
                     MFCLMVGTASLPHLLTRFYSTASVAETRRSVGWSLVFIALLYLSTPALAVLVKYEVLN
                     SLVGSSFDQLPNWIAQWAHVDPALISVQDVNGDGVLQFGEIRLGTDMIMLATPELGGL
                     PYVVSGLVAAGGLAAALSTADGLLLTISNALVHDAMPTDGGQVRVTDNHVILSKFMLL
                     AAAMLAASVAALKPAAILMLVTASFSLAAATFFPSLVLGLFWRGTTARGAVAGMLAGV
                     GLTVGYMLYAAPSLRAWWGLAPDGLWWGIQPISAAFFGVPVGFMTIALVSWWDGRRGV
                     G"
     gene            complement(2705..4660)
                     /locus_tag="PKV30_08405"
     CDS             complement(2705..4660)
                     /locus_tag="PKV30_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRL30270.1"
                     /translation="MFDFIRRHTKLIMGVLFLLIIPSFVLFGLEGYTRFNDGGTKVAT
                     VDGKPITQTEWDNAHRMEVDRLRATNPSLDLSLLDSPALKRASLERLVQERVLAAAAA
                     DEHLMATDARLAADLERDPAIAALRRADGSLDMERYRQLLAAQGLTPEGFEANVRRDL
                     SSRQVLAGVLDSGFASQAQADVAMNAFLERREVRIVPFTPAGYAAKVNPTEAELQAYY
                     QTHVARYQAPESAKVEYIVLDLDSVKKAVSVSEQDLRTYYDQNAATFGAPEQRRASHI
                     LITAPKDAPAAEREKAKSTATALLAQLRQAPGTFADVARKSSQDDASAASGGDLGLFQ
                     RNKGIDPAIAQAAFGLAKAGDLSDLVESDFGYHIVQLAQIQPAAVPPFEQMRAKLEDQ
                     LRTQQAQAKFSELAEEFRNGVYEQSDSLKPVADKLKLTIQTADNVTRTPAPGASGALA
                     NPKFLGALFAADAIDKKRNTEAIDLGGNQLASGRVVSHSAAHARPFAEVQSEVRTAFV
                     NQRAAELARQDGQSRLKAWSDQPASATSLPAAVVLSRDEAQGQPEALVEAVLRADPAK
                     LPSFVGVDLGAGGYALVRVDKVLPRADQTPEQQAQSRTRYDQLWGVAEARSYYDLLKA
                     RYKAEILVPAVAAPTAQSGTPAAAASR"
     gene            complement(4742..4817)
                     /locus_tag="PKV30_08410"
     tRNA            complement(4742..4817)
                     /locus_tag="PKV30_08410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4782..4784),aa:Val,seq:tac)
     gene            complement(4838..5110)
                     /locus_tag="PKV30_08415"
     CDS             complement(4838..5110)
                     /locus_tag="PKV30_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRL30271.1"
                     /translation="MNKTELIEHIANNADISKAAATRALEATIGAVKTTLRKGGTVSL
                     VGFGTFAVGKRAARSGRNPRTGATIKIKAAKVPKFRPGKALKDALN"
     gene            complement(5412..>5631)
                     /locus_tag="PKV30_08420"
     CDS             complement(5412..>5631)
                     /locus_tag="PKV30_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL30272.1"
                     /translation="TLGGSSLLYMLIQRGAAASVTSLMYLVPPTTAAMAWLLFGEPIT
                     LTTVVGIAITALGVSLVVRPAPASSGGR"
BASE COUNT          863 a         1846 c         1890 g         1032 t
ORIGIN      
        1 ccgacgaatt tttgaagttc ttcgacggct tctccatcgg ctccaacgac ctgacgcagc
       61 tcaccctggg cctggatcgc gacagcggca tggaactgct ggccgccgac ttcgacgagc
      121 gcgacccagc cgtcaaggcc atgctgcagc gcgcgattca ggcctgcaag gcacagggca
      181 agtacgtcgg catctgcggc caaggcccga gcgaccaccc agacttcgcg cgctggctgg
      241 ccgacgaggg catcgcctcg atctcgctaa accccgacag cgtgatcgac acctggacgt
      301 tgctggcgac ttgagtcgga tggcacactg aacgatcaat gtcgaacgtg ccgaacacta
      361 taaaaagaga agctgctagc gcagtctggg tgcgagctac gctgcttttt gtttgggtat
      421 tggcatcgtt cggcatggcc ttcttcgcgc gcgatctggg tcagaccgtg gccggctggc
      481 cgctcgcctt ctggctggcc gcgcagggca gcgtgctggt gttcatcggc atcgtcgtgg
      541 tctacgcgtg gtggatgaac cgtcacgaag ccgcaacact ggccggacgc gagggcgact
      601 gaccatgccg ggcgcaccgc gcgccgacct gtggcggctg caccgccatc tgctgctatt
      661 cgtgctcggc ttcatcgcct ttctgctgct gatgcactcg gccgagcgca acggcctgtc
      721 gcgcgactgg atcgggccga tttttttgtt tctctcgatc atgctgtacg cctgcatcgg
      781 gatctactcg cgcaccaccg acccggagga cttctacgtc gccggccgcc gcattcccgc
      841 catgtacaac ggcatggcca cggcggccga ctggatgagc gccgcctcgt tcatcagcct
      901 gtcgggcgcg ctctatctgc aaggtttctc gggcacggcg gcgcagcccg gggggctggc
      961 ctacgtgctg ggctggaccg gcggcttctg cctggtcggg ctgctcatcg cgccgcgcct
     1021 gcgcgccatg gcgctgtaca cggtgcccga cttttatgcc gtgcgcttcg gcggccgatg
     1081 gccgcgcctg ctggccgcgc tgggtgcggt ggtctgctcg ttcatgtatg tggtggcgca
     1141 gatctacggc atcgggctca tcgcttcgcg gctgaccggc gtgcagttcg agatcggcat
     1201 cctgctcggt ttgggcggcg tgctgctgtg cagctttctg ggcggcatgc gcgccatcac
     1261 ctggacgcag gtggtgcagt acgtggtgct gttgctggcg ttcctgattc cggtgtcgtg
     1321 gctggcgtac aagcaactgg gcaacccgct ggcgccattg gcgtatggcg cgcagctgcc
     1381 caagatcgcg caaatggaag agcgcctgat caacgacccg gccgagcgcg aagtgcgcgc
     1441 tgcctttgcg gcgcgcgcgc aggaatacgc agcgcgactg aacgatccag ccaccgcgct
     1501 ggcgcgcgag caccaacggc tcaatgaacg catccgcacg ctgaaggcgc aaggcgccga
     1561 tttttcgctg gtgatggcgg cgcggcgcga actggccgag ctaccgcgcg acgtcgccag
     1621 cgcgcgcgac ttgtggggcc gcgcgatgag cgaggcgcaa acgcgctcgg gcccgctggg
     1681 tggcctgccg cgtcagggcc aggccttcgc cggcgatccg aacggcacgc ccgacgacca
     1741 acgcgcattc caggattcgc gtctgaactt cctggcgctg atgttctgcc tgatggtcgg
     1801 cacggccagc ctgccgcact tgctgacgcg cttttacagc accgccagcg tggctgaaac
     1861 gcggcgctcg gtcggctggt cgctggtgtt cattgcattg ctctacctca gcacgccagc
     1921 gctggcggtg ctggtgaagt acgaggtgtt gaacagcctg gtcggcagca gcttcgatca
     1981 gttgccgaac tggatcgccc agtgggcaca cgtcgacccg gcgctgatct cggtgcagga
     2041 cgtgaacggc gacggcgtcc tgcagtttgg cgaaatccgg ctgggcaccg acatgatcat
     2101 gctggccacg cccgagttgg gcggcctgcc ctacgtggtg tcgggcctgg tggcggcggg
     2161 cggtttggcg gccgcgctgt ccaccgccga cggcctgctg ctgaccatca gcaatgcgct
     2221 ggtgcacgat gccatgccca ccgacggcgg ccaggtgcgg gtgaccgaca accacgtcat
     2281 cctgtccaag ttcatgctgc tggcggcggc catgctggcg gcgtcggtgg cggcgctgaa
     2341 gccagcagcg atcctgatgc tggtgacggc atctttctcg ctcgcggcgg ccaccttttt
     2401 cccctcgttg gtcctgggcc tgttctggcg cggcaccacg gcgcgcggtg ccgtggcggg
     2461 catgctggcg ggcgtggggc tgaccgtggg ctacatgctg tacgcggcgc cgtcgctgcg
     2521 cgcttggtgg ggcctggcac cggacggctt gtggtggggc atccagccca tctcggcggc
     2581 tttttttggc gtgccggtgg gcttcatgac gatcgcgctg gtgtcgtggt gggacgggcg
     2641 gcgcggcgtc ggctgacgcc caaaaaaaag cgccccgaag gacgctgttt ttcgagattc
     2701 ggcgtcagcg cgacgcagcc gccgctggcg taccagattg cgccgtgggc gcagccacgg
     2761 ccggcacaag aatttcggcc ttgtaacggg ctttcagcag gtcgtagtag ctgcgcgctt
     2821 cagcgacacc ccacaactgg tcatagcgcg tgcggctttg cgcctgctgc tcgggtgtct
     2881 gatcggcacg cggcaggacc ttgtcaacgc gcaccagcgc gtagccaccc gcccccagat
     2941 cgacgccaac gaaggacggc agtttggcgg gatcggcacg cagcacggct tcgaccagcg
     3001 cttccggctg gccttgcgct tcgtcgcgcg agagcaccac agcggctggc aatgaggtgg
     3061 ccgacgcggg ctgatcgctc cacgccttca ggcgcgactg accgtcctgg cgcgccagct
     3121 cggcagcgcg ttggttgaca aaggcggtgc gcacctcgga ctgcacttcg gcaaacggcc
     3181 gtgcgtgggc ggcgctgtgc gacaccacgc ggcccgacgc cagttggttg ccgcccagat
     3241 cgatcgcctc ggtgttgcgc ttcttgtcta tcgcatcggc ggcgaacagc gcgcccagaa
     3301 acttgggatt cgccagcgcg ccgctggcgc cgggcgccgg cgtgcgggtc acgttgtcgg
     3361 ccgtctggat ggtgagcttg agcttgtccg ccaccggctt caagctgtcc gactgctcgt
     3421 aaacgccgtt gcgaaactcc tcggccagtt cgctgaactt ggcctgcgcc tgctgcgtgc
     3481 gcaactggtc ctcgagcttg gcgcgcatct gctcgaatgg cggcaccgct gccggctgaa
     3541 tctgggccag ctggacgatg tggtagccga aatcgctttc gaccagatcg ctcaagtcgc
     3601 ccgccttggc caggccgaag gcggcctgcg cgatcgccgg atcgatgccc ttgttgcgct
     3661 gaaacaggcc caggtcgccg ccgctggccg cgctggcatc gtcttgcgac gacttgcgcg
     3721 ccacgtcggc aaaagtgccc ggcgcctggc gcagttgcgc cagcaaggcg gtggccgtgc
     3781 tcttggcttt ctcgcgttca gccgccggcg catccttggg cgccgtgatc aggatgtggc
     3841 tggcgcgccg ctgctcgggc gcgccaaagg tggcggcatt ctggtcgtaa taggtgcgca
     3901 agtcctgctc gctcacgctc acggccttct tcacgctgtc cagatccagc acgatgtact
     3961 cgaccttggc cgattcgggc gcctggtagc gcgcgacgtg cgtctggtag taggcttgca
     4021 gctcggcctc cgtcggattg accttggcgg cgtagccagc tggcgtgaac ggcacgatgc
     4081 gcacttcgcg ccgctccaga aacgcattca ttgccacatc ggcctgcgcc tgactggcga
     4141 aaccgctgtc cagcacaccc gccagcacct ggcgcgacga cagatcgcgc cgcacgttgg
     4201 cctcgaatcc ttcgggtgtg agcccctgcg cggccagcag ctggcggtaa cgctccatgt
     4261 ccagactgcc gtcggcgcgg cgcagcgcgg caatggccgg gtcacgctcc aggtcggccg
     4321 ccagccgcgc atcggtcgcc atcaggtgct cgtcggccgc tgcggcggcc agcacgcgct
     4381 cttgcaccaa acgctccagc gaggcgcgct tgagcgcggg cgaatccagc aacgacaggt
     4441 ccagcgaggg gttggtggcg cgcaggcgat ccacttccat gcggtgcgcg ttgtcccatt
     4501 cggtctgcgt gatgggcttg ccgtcgacag tggccacctt ggtgccgcca tcgttgaacc
     4561 gcgtatagcc ttcgaggccg aacagcacaa aagaaggaat gatcagcaaa aacagcacac
     4621 ccatgatgag cttggtgtgc cggcggatga agtcgaacat gcgcgaaact ctcgatcgat
     4681 acggtcgcgt gaaaatgaaa aaggcgaacc ggggttcgcc tttgcgcaac gggtagggac
     4741 ttggtgggtg ctgaggggct cgaacccccg acctacgcct tgtaagggcg ccgctctacc
     4801 aactgagcta agcaccccac ctgactgagc aaaaagatca gttcagcgcg tccttcaacg
     4861 ctttgccagg acggaacttg ggcaccttgg ctgccttgat tttaatggtt gcgccggtgc
     4921 ggggattgcg acccgagcgc gccgcacgct tgccgacggc aaaggtgcca aaacccacca
     4981 gcgacacggt gccgcccttg cgcagcgtgg tcttgacggc gccgatggtg gcttccagcg
     5041 cgcgggtggc tgcggccttg gaaatgtcgg cgttgttggc gatgtgctcg atcagctcgg
     5101 ttttgttcac gaagttgtcc ctctctcaag aagattcaag aatccggaaa gcgactttgg
     5161 tgtcggtcgg cgatctggcc tgcccccgcg gtctgcttga cgcgccacag acggcttggc
     5221 gatcggtgcg gacccggcga gagcgcccgg ccacgtcgac aaacccctgt cggcgagcga
     5281 tgcattctag acgcaattgc ctgctgtaaa gcccgtgcat cccctgcttg tgcgatgacg
     5341 caacaacctg cggaaaaaat tccggccgcg ccgcgtgaca gtttcaacgg atggccgccg
     5401 ccgcacccgt ttcagcgccc gccgcttgac gccggcgccg gccgcaccac caggctgacc
     5461 cccagcgccg tgatggcgat gccgaccacg gtggtcagcg tgatcggctc gccaaacagc
     5521 agccacgcca tcgccgccgt ggtgggtggc accaggtaca tcaggctggt caccgacgcc
     5581 gcggcgccgc gctgaatgag catatacaac agcgagctgc cgcccagcgt c
//
LOCUS       DAOMVO010000156         5606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_3974_length_5606_cov_17.036030, whole genome shotgun sequence.
ACCESSION   DAOMVO010000156 DAOMVO010000000
VERSION     DAOMVO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_5606_cov_17.036030"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /gene="mnmA"
                     /locus_tag="PKV30_08425"
     CDS             <1..974
                     /gene="mnmA"
                     /locus_tag="PKV30_08425"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRL30273.1"
                     /translation="EIEHVNFAADYKDRVFAEFLREYSAGRTPNPDVLCNAEIKFKAF
                     LDHAMRLGAEKIATGHYARTRFNEATQKHELLKGLDPAKDQSYFLHRLTQAQLARTLF
                     PIGELHKTEVRGIASEIGLPNAKKKDSTGICFIGERPFREFLNRYLQTQPGPIKDARG
                     RQIGEHVGLSFYTLGQRQGLGIGGVKDKGAQRGGGDHAPWFVARKDMATNTLYAVQGH
                     DHPWLLSRDVVFDDTAWTSGAAPSDGAFAAKTRYRQQDAACALSAGDAPGAFCLRFAE
                     PQWAVTPGQSAVLYDGEVCLGGGVIASAVDSEEKRPPALSGQAPAAL"
     gene            complement(993..1805)
                     /locus_tag="PKV30_08430"
     CDS             complement(993..1805)
                     /locus_tag="PKV30_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRL30274.1"
                     /translation="MTPSTAWTRRAWLAGAAALLTACASPTAPPPDSAAREVRVVSSG
                     GFTAAYKILAPLYAQASGVKIVSEYGASIGNAPDSIPSRLARGEKFDIVILADSAFEK
                     LVAEGKGVAGSRVDLGRSLIGASVKKGTPKPDISTVPALKNALLNAKSVAYSASASGT
                     YLSEELFAKLGVADRMKVTAKKIMSERVGAVVARGDAELGFQQASELLPFTELDYLGP
                     IPDEVQQKVFFSAGVLQGAPNPEGAKRFIRYLASPAAAGVIESTGLTPVAKR"
     gene            complement(1850..2119)
                     /locus_tag="PKV30_08435"
     CDS             complement(1850..2119)
                     /locus_tag="PKV30_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRL30275.1"
                     /translation="MKVKGLDIVWLLLLAATAFTWWLGHSGALVPPRLGMMALVFGLA
                     WLKGLGVILEFMELRHAPPAWRWALIGGLTLIVGLILLAYWLAEP"
     gene            complement(2116..2691)
                     /locus_tag="PKV30_08440"
     CDS             complement(2116..2691)
                     /locus_tag="PKV30_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRL30276.1"
                     /translation="MSTATRTAPRLPGDLFIWYVILLEGATFCVLFGAFAFTRARHLE
                     LFSSSQQQLDLSAGAINTALLLTASWCVARAVHAVRAGARGAGLRWLIAGMLGGAGFV
                     ALKLHEYADKAAQGIGLSTNLFFDFYFILTGFHLLHVLAGLLALGLVGVGLLRQPRGA
                     PNAHALETAAAFWHLVDLLWLVLFPLVYVMR"
     gene            complement(2783..3802)
                     /locus_tag="PKV30_08445"
     CDS             complement(2783..3802)
                     /locus_tag="PKV30_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRL30277.1"
                     /translation="MPQRPDALKHLAPNWFASVMGLAGLSLAWGRAEPILGEMAGAGA
                     LVLAAAAALLFVALAVLSWIRWQHYPEALAADLKHPVRHAFIATIPVSIILLATCGVS
                     LLGTSAWLAALWWLGSLLQLFTTLWVATRWLSADKAQSLSWAALTPVLLIPVVGNVLA
                     PLAGVSLGAGAWAAAQFGIGLLLWPVLLALILARIAAQGLWAERLLPATFISIAPPAV
                     IGSAALQLGGPPLLAWMCWGVALFFLLWSLQIGRRLLAQPFSIGFWSIGFPLAAFATL
                     TLRLAQQHSAAQAPAMIALALASLVIVLLGIATWKSWREGSLLQAEPVALIAVAGSDQ
                     PPRPQ"
     gene            4107..5126
                     /locus_tag="PKV30_08450"
     CDS             4107..5126
                     /locus_tag="PKV30_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL30278.1"
                     /translation="MNHRRLALHTLATAALLAGAPFAHAQQPAAVTLLNVSYDPTREF
                     YVDFNKAFAKHWKAKTGQDVTVKQSHGGSGKQARSIIDGLQADVATLALAGDTDALVT
                     HGALIPKDWQKRLPHNSSPYTSTIVLVVRAGNPKGIKDWDDLVKPGVKVITPNPKTSG
                     GARWNYLAAWEYAKRKAGGDDAKAKAFVAQLYQNVPVLDTGARGSTITFGQRAQGDVL
                     IAWENEAHLLEKEFGAKFDIVYPSLSILAEPAVTVVDKNVDKHGTRAVAQAYLDYLYT
                     DEGQDLAGKHFYRPISDKAAAKYARQFPKLNLFTIDQAFGGWTQAGKTHFADGGQFDQ
                     IYTNK"
     gene            5160..5369
                     /locus_tag="PKV30_08455"
     CDS             5160..5369
                     /locus_tag="PKV30_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30279.1"
                     /translation="MPQPITRPLPPTALRVLAATADAWGLCMCAPLPAIRTRAAPRHA
                     AHSSPKELRQWPYWPDYPSAAIGWR"
BASE COUNT         1046 a         1971 c         1808 g          781 t
ORIGIN      
        1 tcgagatcga gcacgtcaac ttcgcggccg actacaagga ccgcgtgttt gccgaattcc
       61 tgcgcgaata ctcggccggc cgtacgccca acccggacgt gctgtgcaat gccgagatca
      121 agttcaaggc tttcctcgac cacgcgatgc gcctgggcgc cgaaaagatc gccacgggtc
      181 attacgccag gacgcgcttc aacgaagcaa cccaaaagca cgaactgctc aaaggcctag
      241 accccgccaa ggaccagagc tatttcctgc accgcctgac gcaggcgcaa ctggccagga
      301 cgctgtttcc gatcggcgag ctgcacaaga ccgaggtgcg gggcatcgcc agcgaaatcg
      361 gcttgcccaa cgcgaagaaa aaggattcaa ccggcatctg cttcatcggc gagcgaccgt
      421 tccgcgaatt cctcaaccgc tatttgcaaa cccagcccgg cccgatcaag gacgcgcgcg
      481 gccgccagat cggcgagcac gtgggcctgt ccttctacac gctgggccag cggcagggcc
      541 tgggcatcgg cggcgtgaag gacaaaggcg cgcagcgcgg cggcggcgat cacgcgccgt
      601 ggttcgtggc gcgcaaggac atggcgacca acacgctgta cgccgtgcaa ggccacgacc
      661 acccgtggct gctgtcgcgc gacgtggtgt ttgacgatac cgcgtggacg tcgggcgccg
      721 cgccttcaga cggcgccttt gccgccaaga cgcgctaccg ccagcaggac gcggcctgcg
      781 cgctgtcggc gggcgacgcg cccggcgcct tttgcctgcg ctttgccgag ccgcaatggg
      841 ccgtcacgcc gggccagagc gcggtgctgt acgacggcga ggtgtgcctg ggcggcggcg
      901 tgatcgcgtc ggccgtggat tcagaagaaa aacggcctcc agcgctttct ggacaagcgc
      961 cggcagctct ttaattcata gcgaagcggc gttcagcgct tggccaccgg cgtcagcccg
     1021 gtcgattcga tcaccccggc cgccgctggc gacgccagat agcggatgaa gcgcttggcg
     1081 ccctccggat tgggcgcgcc ctgcagcacg ccggccgaga agaagacctt ttgctgcacc
     1141 tcatccggaa tcggccccag gtaatccagc tcggtgaagg gcagcaactc gctggcctgc
     1201 tgaaagccca gttcggcatc gccgcgcgcc accacggcgc cgacgcgctc gctcatgatc
     1261 tttttcgccg tcaccttcat gcggtcggcc acgcccagct tggcaaacag ctcttccgac
     1321 aaataggtgc cgctggcgct ggccgaatag gcgaccgact tggcgttcag cagcgcgttc
     1381 ttcagcgccg gcacggtgct gatgtcgggc ttgggggtgc ctttcttgac cgacgcgccg
     1441 atcagcgagc gccccaggtc gacgcggctg cccgccacgc ccttgccttc ggccaccagc
     1501 ttttcgaagg ccgaatcggc caggatgacg atgtcgaact tctcgccgcg cgccagcctg
     1561 ctggggatgg aatccggcgc gttgccgatc gaggcgccgt attcgctgac gatcttgacg
     1621 ccgctggcct gcgcatacag cggcgccagg atcttgtagg cggcggtgaa gccgcccgag
     1681 ctgaccacgc gcacctcgcg cgcggcgctg tccggcggtg gcgcggtcgg gctggcgcag
     1741 gcggtgagca gcgcggcggc gccggccagc caggcgcggc gcgtccaggc ggtggatggg
     1801 gtcatggctt gtctcctctg tggtgcgtgg tgcaatgtgg cgtcgcgcct cagggctcgg
     1861 caagccagta ggccagcagg atcagcccga cgatcagcgt cagcccgccg atcagcgccc
     1921 agcgccaggc cggcggggcg tggcgcagtt ccatgaactc caggatcacg cccaagccct
     1981 tgagccacgc caggccaaac accagcgcca tcatgcccag ccgcggcggc accagcgcgc
     2041 cgctgtggcc cagccaccag gtgaaggcgg tggcggccag cagcagcagc cagacgatgt
     2101 cgaggccttt gactttcatc gcatcacgta gaccagcgga aacagcacca gccacagcag
     2161 atcgaccagg tgccagaagg cggctgcggt ctccagcgca tgggcgttgg gcgcgccgcg
     2221 cggctggcgc agcaggccca cgccgaccag ccccagtgcc agcaagccgg ccagcacgtg
     2281 cagcaggtga aagccggtca ggatgaagta gaagtcgaag aacaaattgg tcgacaggcc
     2341 aatgccttgg gccgccttgt cggcgtactc gtgcagcttg agcgcgacga aacccgcacc
     2401 gcccaacatg ccggcgatga gccagcgcaa gccggcgccg cgcgcgccgg cccgcaccgc
     2461 gtgcaccgcg cgcgccacgc accagctggc cgtgagcagc aacgcggtgt tgatggcgcc
     2521 ggcgctcaga tcaagctgct gttgcgagct gctgaacagc tccaggtggc gcgcccgcgt
     2581 gaaggcgaag gcgccgaaca gcacgcaaaa cgtcgcgcct tcgagcagga tgacgtacca
     2641 gatgaacaaa tcgcccggca ggcgcggtgc ggtgcgggtg gccgtgctca tggcgtggtg
     2701 tcgtgacttg tcacttctga tttcatagct gctcgcgctt gcctgacaag cgctggagcc
     2761 gaaaaatcct tcaagtcgac catcactgcg ggcgcggcgg ctggtcgctg ccggccaccg
     2821 cgatcagcgc caccggctcg gcctgcagca ggctgccttc gcgccagctc ttccaggtgg
     2881 cgatgcccag cagcacgatg accagcgacg ccagcgccag cgcgatcatg gccggcgcct
     2941 gcgccgcact gtgctgctgc gccagccgca acgtcagcgt ggcaaatgcc gccagcggaa
     3001 agccgatcga ccagaagccg atcgaaaacg gctgcgccag caagcgccgg ccgatctgca
     3061 ggctccacag caagaagaac agcgccacgc cccagcacat ccacgccagc agcggcgggc
     3121 cgcccagttg cagcgccgcc gagccaatca ccgccggcgg cgcgatgctg atgaaggtgg
     3181 ccggcagcag ccgctcggcc cacagccctt gcgcggcgat ccgcgccagg atcagcgcca
     3241 gcagcaccgg ccacaacagc agcccgatgc cgaactgcgc cgccgcccag gcgcccgcgc
     3301 cgaggctgac gccggccagc ggcgccagca cgttgccgac cacgggaatc agcagcactg
     3361 gcgtcagcgc ggcccagctt aaagactgcg ccttgtcggc gctcagccag cgcgtggcca
     3421 cccacagcgt ggtgaagagt tgcagcagcg aacccagcca ccacagcgcg gccagccacg
     3481 cgctggtgcc gagcagcgac acgccgcaag tggccagcag gatgatcgac accggaatcg
     3541 tggcgatgaa ggcatggcgc accgggtgct tcaagtcggc cgccagcgcc tcggggtagt
     3601 gctgccagcg aatccacgac agcaccgcca gcgcgacaaa cagcagcgcc gccgccgccg
     3661 ccagcaccag cgcgccggcc cccgccatct cgcccaggat cggctcggcg cgcccccacg
     3721 ccagcgacag cccggccagc cccatgacgc tggcaaacca gttcggtgcg aggtgcttga
     3781 gcgcgtcggg tctttggggc atggggcgat gatgccacgc acgcgactat gtttttaata
     3841 gctgcttgcg cagtagggac gtgcgccaaa gcccattttc acttgaagcc gcgcagcagg
     3901 cgagcaccga aggccgcgga gcaggtcacg ccggaacgcc gtgctcgggg ttcccccgag
     3961 cgccagcgtt gtccccctca gggggaaggc gccgcaggcg cctcaggggg agcgttataa
     4021 gcaaacaaga aatcaatcgt tccttttgcg gccggcggct ttcatcatcg cggctctgtt
     4081 attcagataa agctggagcc cgacccatga accaccgccg ccttgcgctg cacaccctcg
     4141 ccaccgctgc cctgctggct ggcgcaccct tcgcccacgc gcagcaaccc gccgccgtga
     4201 ccctgctcaa cgtcagctac gaccccacgc gcgagttcta cgtcgacttc aacaaggcgt
     4261 ttgccaaaca ctggaaggcc aagaccgggc aggacgtgac ggtgaagcaa tcgcacggcg
     4321 gctcgggcaa gcaggcgcgc tcgatcatcg acgggctgca agccgacgtc gccacgctgg
     4381 cgctggccgg cgacaccgat gccctcgtca cgcacggcgc gctgatcccc aaggactggc
     4441 aaaagcgcct gccgcacaac agctcgccct acacctcgac catcgtgctg gtggtgcgcg
     4501 cgggcaaccc gaagggcatc aaggattggg acgacctggt caagcccggc gtgaaggtca
     4561 tcacccccaa ccccaagacc agcggcggcg cgcgctggaa ctacctcgcc gcgtgggaat
     4621 acgccaagcg caaggccggc ggtgacgacg ccaaggccaa ggccttcgtc gcccagctgt
     4681 accagaacgt gcccgtgctc gacaccggcg cgcgcggctc gaccatcacc tttggccagc
     4741 gcgcgcaggg cgacgtgctg attgcgtggg agaacgaggc gcatctgctt gaaaaagagt
     4801 tcggtgccaa gttcgacatc gtctacccca gtctctcgat cctggccgag ccggcggtga
     4861 cggtggtcga caagaacgtc gacaagcacg gcacccgcgc cgtggcgcag gcgtatctgg
     4921 attaccttta caccgacgag gggcaggatt tggccggcaa gcacttctac cgccccatca
     4981 gcgacaaggc cgcggccaag tacgccaggc agttccccaa gctcaacctg ttcaccatcg
     5041 accaggcctt tggcggctgg acgcaagctg gcaaaacgca ctttgccgac ggcggccagt
     5101 tcgaccagat ctacaccaac aagtaagccg ctttcacccc accccaagag gagttcgcca
     5161 tgccccagcc catcacccgc ccgttgccgc ccaccgccct gcgcgtgctg gccgccaccg
     5221 ccgatgcgtg gggcctgtgc atgtgcgcgc ccttgccggc gatccgcacg cgcgccgcac
     5281 cgcgccacgc agcgcacagc tcgccaaagg aactgcgcca gtggccgtat tggccggact
     5341 accccagcgc cgcgattggt tggcgatgaa ttgacccgga gcgatttcaa gccaaaggcg
     5401 ccgccagcgc ttgaagaaca agcgcgagca gctacgattt ttgaactggg ttgccctcac
     5461 cttgcttcga caagctcggg aaagaccaac cctttcccag aagcagaggg agaaaaccca
     5521 aggccgagcg cagcgatggc ccgaatgggt ttcaaccccc ttctggccgt gccgagaagc
     5581 gcagggcgtg gggcgggcgc gcagcg
//
LOCUS       DAOMVO010000157         5484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4091_length_5484_cov_16.725732, whole genome shotgun sequence.
ACCESSION   DAOMVO010000157 DAOMVO010000000
VERSION     DAOMVO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_5484_cov_16.725732"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="PKV30_08460"
     CDS             <1..102
                     /locus_tag="PKV30_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HRL30280.1"
                     /translation="AAVRNHILQLRGIDTYQEFVDRHRRAMEKGNES"
     gene            complement(167..607)
                     /gene="fur"
                     /locus_tag="PKV30_08465"
     CDS             complement(167..607)
                     /gene="fur"
                     /locus_tag="PKV30_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HRL30281.1"
                     /translation="MDTLDELKSTGLKATLPRLKILEIFQKAEQRHMTAEDVYRLLLA
                     EHSDIGLATVYRVLMQFEQAGLLSRNHFEAGKAVYELREDEHHDHLVCLDCGHVEEFH
                     DPEIEKRQHAVAKAKGFLIADHALSLYANCTKADCPHRPAGSRR"
     gene            681..1244
                     /locus_tag="PKV30_08470"
     CDS             681..1244
                     /locus_tag="PKV30_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRL30282.1"
                     /translation="MSLTSRIPARAIAGAMLAVLTAALGACSSFEQGTRGIADALTLY
                     KPEVVQGNFVSKEQVAALQPGMSRVQVRDTLGTPLVTSVFHADRWDYVFTMRRQRVEP
                     QNFRLTVFFKGDALERFEGDEMPSETEFVQRISRERKVKVPVLEATEDQLAKFPAAPT
                     DAAPAAGDAATSTAVPALSYPPLEPGR"
     gene            1306..2124
                     /gene="dapB"
                     /locus_tag="PKV30_08475"
     CDS             1306..2124
                     /gene="dapB"
                     /locus_tag="PKV30_08475"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRL30283.1"
                     /translation="MTDSSPPIRVAIAGATGRMGQMLIEAVRASGDCQLAGALDRADS
                     PALGQDAGAFAGWASGVPVQAALDAGLQNAQVLIDFTRPEATVAHLAACRRLGVALVI
                     GTTGFTDAQKADIAAAAQDIAIVMAPNMSVGVNVTFKLLEMAAKALSTGYDIEIIEAH
                     HRHKVDAPSGTALKMGEVIADALGRELKDCAVYERHGHTGERDPSTIGFAAIRGGDIV
                     GDHTVLFAGTGERIEISHKASSRAGYAQGSLRAVRFLAGTKSGLFDMFDVLKLR"
     gene            2153..2791
                     /locus_tag="PKV30_08480"
     CDS             2153..2791
                     /locus_tag="PKV30_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRL30284.1"
                     /translation="MNLQHVFAQSDAIGKAVAILLLLMSIASWVVILWKAWLLRRAGG
                     DVVRSVAAFWQAPSLDDAASRVGGFDRGQLVLPLVQATQLPAGGTLAAAGDRAQQLTR
                     ALRDALHGIMGRLQFGQVLLATVGSIAPFVGLLGTVWGIYHALISIAGSGQISIEKVA
                     GPVGEALIMTAAGLAVAIPAVLAYNWFGRIIGRIEAELEGFARDLRELLAKH"
     gene            2920..3375
                     /locus_tag="PKV30_08485"
     CDS             2920..3375
                     /locus_tag="PKV30_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRL30285.1"
                     /translation="MAFGRLERTKGSEPMSDINVTPLVDVMLVLLVIFIITAPLLASA
                     IRLDLPKTDAAQPGDPPKFVTVVVDKAGQAFFNDKPVAQPELAAELLAAAKANPETEV
                     QLRADQGVPYGRVVEVMGAAQKAGLNRIGFVAEAPSADAAPAPTPAASN"
     gene            3519..>5484
                     /gene="leuS"
                     /locus_tag="PKV30_08490"
     CDS             3519..>5484
                     /gene="leuS"
                     /locus_tag="PKV30_08490"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRL30286.1"
                     /translation="MQEKYDHLDVEQSAQAHWRARDAYRVHEDARNARGEAKPKYYAC
                     SMLPYPSGKLHMGHVRNYTINDMLARQLRMKGYNVLMPMGWDAFGLPAENAALKNGVP
                     PAKWTYENIATMKGQMQAMGLAIDWSREVASCDASYYKWNQWLFLKMLEKGIAYRKTQ
                     VVNWDPVDMTVLANEQVIDGRGWRTGALVEKKEIPGYYLKITDYAQELLDHVQIGNPK
                     ATLSGWPDRVRLMQENWIGKSEGVRFAFTHDIRGDDGAPIGDGKMYVFTTRADTIMGV
                     TFCAVAPEHPLATQAAQNNPELAAFIERCKQGGTTEAELAARDKEGMPTGLTVTHPLT
                     GEAVPLWVGNYVLMSYGDGAVMGVPAHDERDFAFALKYGLPILQVVHVDGQEYDYTRW
                     HDWYADKAAGITINSDNFSGLSYSQAVDAVAHALAEKGLGEKKTTWRLRDWGISRQRY
                     WGTPIPMIHCATCGTVPVPEKDLPVVLPLDLVPDGSGNPLNKSEAFLNVPCPCCGQPA
                     RRETDTMDTFVDSSWYFMRYCDPTDAEAMVAGGTRYWMPMDQYIGGVEHAILHLLYAR
                     FWTKVMRDLGLVNFDEPFTRLLTQGMVLNHIFFERGAKGEMNYHPPETVTPVLDADGH
                     LVGGKLADGTLLEYGGVGKMGKTERNGVDPQ"
BASE COUNT          945 a         1857 c         1824 g          858 t
ORIGIN      
        1 gcggcggtgc gcaaccacat cctgcagttg cgcggcatcg acacctacca ggaattcgtc
       61 gaccgccatc ggcgtgcgat ggaaaaaggc aacgagtctt gatgcttttg ttttgatagc
      121 tgcttgcgct tgctggacaa gcgccaccgc cgcttttgac ttgtattcag cggcgcgagc
      181 cggccggccg gtgcgggcaa tcggccttgg tgcagttggc gtacaggctg agcgcgtgat
      241 cggcaatcag gaaacccttg gccttggcca ccgcgtgctg gcgcttctcg atctcgggat
      301 cgtgaaactc ctccacgtgg ccgcagtcca ggcacaccag atggtcgtgg tgctcgtcct
      361 cgcgcagctc gtacacggcc ttgccggcct cgaaatggtt gcgactcagc agcccggcct
      421 gctcgaactg catcagcacg cggtacacgg tggccaggcc gatgtccgaa tgctcggcca
      481 gcagcaggcg gtacacgtct tcggccgtca tgtggcgttg ctcggccttc tggaaaatct
      541 ccagaatctt caggcgcggc agcgtggctt tcaggccggt gctcttgagc tcgtctaagg
      601 tgtccattcc aatgaggtga gccgcgcgcc gtggcgccgc cgctacaatg atcggcccta
      661 ttgtgcatga gcagcgacgc atgtctctca cttcccgaat ccctgcccgc gccatcgccg
      721 gcgccatgct ggccgtgctg acggcggcgc tgggcgcctg cagcagcttc gagcaaggca
      781 cgcgcggcat tgccgacgcg ctgacgctgt acaaacccga ggtggtgcag ggcaacttcg
      841 tgtcgaaaga gcaggttgcc gctctgcagc ccggcatgtc gcgcgtgcag gtgcgcgaca
      901 cgctgggcac gccgctggtc accagcgtgt tccacgccga ccgctgggac tacgtgttca
      961 ccatgcgccg ccagcgcgtc gagccgcaga acttccgcct gacggtgttc ttcaagggcg
     1021 atgcgctgga gcgcttcgag ggcgacgaaa tgcccagcga aaccgagttc gttcagcgca
     1081 tcagccgcga acgcaaggtg aaggtgccgg tgctggaagc caccgaggat cagctggcga
     1141 aattccccgc cgccccgacc gatgccgcac ccgccgccgg cgacgcagcc accagcaccg
     1201 ctgtgccggc gcttagctat ccaccgctgg agccggggcg ctgagcgccg ccgccacgcc
     1261 ggcgcgcctg gcggccggct gcccaccaag tccttgagct tgtccatgac cgattcttcc
     1321 ccgcccatcc gcgtcgccat tgccggcgcg accggccgca tgggtcagat gctgatcgag
     1381 gccgtgcgcg cctcgggcga ttgccagctg gccggtgcgc tggaccgtgc cgacagcccc
     1441 gcgcttggcc aggacgccgg tgcctttgcc ggctgggcct cgggcgtgcc ggtgcaggcc
     1501 gcgctggacg ccggcctgca gaacgcgcag gtgctgatcg acttcacccg gcccgaagcc
     1561 accgttgcgc acctggccgc ctgccgccgg ctgggcgtgg cgctggtcat cggcaccacg
     1621 ggcttcaccg acgcgcaaaa ggccgacatt gccgccgccg cgcaagacat cgccatcgtc
     1681 atggcgccca acatgagcgt cggcgtcaac gtcaccttca agctgttgga gatggccgcc
     1741 aaggcgctgt cgaccggcta cgacatcgag atcatcgagg cgcatcaccg ccacaaggtc
     1801 gatgcgccga gcggcaccgc gctgaagatg ggcgaggtga tcgctgacgc gctggggcgt
     1861 gaactgaagg attgcgccgt gtacgagcgc cacggccaca ccggcgagcg cgatccctcc
     1921 accatcggtt ttgccgccat ccgcggcggc gacatcgtgg gcgaccacac cgtgctgttt
     1981 gccggcaccg gcgagcgcat tgaaatcagc cacaaggcca gcagccgcgc cggctacgcg
     2041 cagggcagcc tgcgcgcggt gcgcttcctg gcgggcacga aaagtggcct gttcgatatg
     2101 ttcgacgtgc tgaagctgcg ttgaacctca agagttttcc tttcaatcca agatgaacct
     2161 gcaacacgtt ttcgcgcaga gcgacgccat cggcaaggcc gtggccatcc tgctgctgct
     2221 catgtccatc gccagctggg tggtgattct gtggaaggcc tggctgctgc ggcgcgccgg
     2281 cggcgacgtg gtgcgcagcg tggcggcgtt ctggcaggcg ccgtcgctgg acgatgcggc
     2341 cagccgcgtc ggcggcttcg accgcggcca gctggtgctg ccactggtgc aggccacgca
     2401 actgccggcc ggcggcacgc tggcggcggc gggcgaccgc gcgcagcagc tcacgcgtgc
     2461 gctgcgcgat gcgctgcacg gcatcatggg gcggctgcag ttcggccagg tgctgctggc
     2521 caccgtcggc tcgattgcgc cctttgtcgg cctgctgggc accgtgtggg gcatctacca
     2581 tgcgctgatc agcatcgccg gctcgggcca gatcagcatc gagaaggtcg ccggcccggt
     2641 gggcgaggcg ctgatcatga ccgccgccgg cctggcggtg gccattccgg ccgtgctggc
     2701 ctacaactgg ttcggccgca tcatcggccg catcgaggcc gagctggaag gctttgcgcg
     2761 cgacttgcgg gaactgctgg cgaaacattg agccaccggg ctgtgctttt caacttattc
     2821 aacaccctcc ggacgccgcc gggccgtccc aaggcggccg gggcccccct ggggggcagc
     2881 gcagcacgcg aagcggcgaa gcgtgggggc aggtgactta tggcatttgg acgactcgaa
     2941 cgcaccaagg gctccgagcc catgagcgac atcaacgtca cgccgctggt cgacgtgatg
     3001 ctggtgctgc tggtgatctt catcatcacc gcgccgctgc tggccagcgc gatccggctc
     3061 gatctgccca agaccgacgc ggcgcagccg ggcgatccgc ccaagttcgt caccgtggtg
     3121 gtcgacaagg ccggccaggc cttcttcaac gacaagccgg tggcgcagcc tgagttggcg
     3181 gccgagctgc tggccgccgc caaggccaac cccgagaccg aggtgcagct gcgcgccgac
     3241 cagggcgtgc cctacggccg cgtggtcgag gtgatgggcg cggcgcaaaa ggccggcctc
     3301 aatcgcatcg gcttcgtggc cgaggcgccg agcgccgacg cggcgccagc gccgaccccc
     3361 gccgcttcca actgaggcac tgcggcgccg cgtgccgctt tccagcacga ttttggccgc
     3421 cagcgcttga tggacaagcg cgagcagcta tcaaaagagt agttcaacag atgcgccgcc
     3481 cggccggccg cacggccgcg cccgcctaca atttgcccat gcaagagaaa tacgatcatc
     3541 tggacgttga gcagtccgcc caggcgcact ggcgcgcgcg cgacgcctac cgcgtgcacg
     3601 aggacgcgcg caacgcgcgg ggcgaggcaa aacccaagta ctacgcctgc tccatgctgc
     3661 cctaccccag cggcaagctg cacatgggcc acgtgcgcaa ctacaccatc aacgacatgc
     3721 tggcgcgcca gctgcgcatg aagggctaca acgtcctcat gcccatgggc tgggacgcct
     3781 ttggcctgcc ggctgagaac gcggcgctga aaaacggcgt gccgccggcc aagtggacgt
     3841 acgagaacat cgccaccatg aagggccaga tgcaggccat ggggctggcc atcgactgga
     3901 gccgcgaagt cgccagctgc gacgccagct actacaagtg gaaccagtgg ctgtttctga
     3961 agatgctgga aaaaggcatc gcctaccgca agacccaggt tgtcaactgg gacccggtgg
     4021 acatgacggt gctggccaac gagcaggtga tcgacggtcg cggctggcgc accggcgcgc
     4081 tggtcgagaa gaaggagatc ccgggctact acctcaagat caccgactac gcgcaagaac
     4141 tgctcgacca cgtgcagatc ggcaacccca aggccacgct ctctggctgg ccggatcgcg
     4201 tgcggctgat gcaggaaaac tggatcggca agagcgaagg cgtgcgcttt gccttcacgc
     4261 acgacattcg cggcgacgac ggcgcgccga tcggcgatgg aaagatgtac gtcttcacca
     4321 cgcgcgccga caccatcatg ggcgttacct tctgcgccgt cgcgcccgag cacccgctgg
     4381 ccacgcaggc cgcgcagaac aatcccgaac tcgccgcctt catcgaaaga tgcaagcaag
     4441 gcggcaccac cgaggccgaa ctcgctgcgc gcgacaagga aggcatgccc accggcctga
     4501 cggtgacgca tccgctgacc ggcgaggcgg tgccgctgtg ggtcggcaac tacgtgttga
     4561 tgagctatgg cgacggcgcc gtgatgggcg tgcccgcgca cgatgagcgc gacttcgcct
     4621 ttgcgctgaa gtacggcctg cccatcctgc aggtggtgca tgtggacggc caggaatacg
     4681 actacacccg ctggcacgac tggtacgccg acaaggcggc gggcatcacc atcaattccg
     4741 acaatttcag cggcttgtcg tattcacagg ccgtcgatgc cgtggcgcac gcgctggccg
     4801 aaaaaggcct gggcgagaaa aaaaccacct ggcgcctgcg cgactggggc atcagccgcc
     4861 agcgctactg gggcacgccg attcccatga tccactgcgc cacctgcggc acggtgccgg
     4921 tgcccgagaa agacctgccc gtggtgctgc cgctcgacct ggtgcccgac ggcagcggca
     4981 acccgctgaa caagagcgaa gcgttcctga acgtgccttg cccctgctgc gggcagccgg
     5041 cgcggcgcga gaccgacacc atggacacgt tcgtggattc gtcgtggtac ttcatgcgct
     5101 actgcgaccc gaccgatgcc gaggccatgg tggccggcgg cacgcgctac tggatgccga
     5161 tggaccagta catcggcggc gtcgagcacg ccatcctgca cctgctgtac gcgcgcttct
     5221 ggaccaaggt gatgcgcgat ctggggctgg tgaacttcga cgagcccttc acccgcctgc
     5281 tgacgcaggg catggtgctc aaccacatct tcttcgagcg gggcgccaag ggcgagatga
     5341 actaccaccc gcccgaaacc gtgacgccgg tgcttgacgc cgacggccac ctggtcggcg
     5401 gcaagctggc cgacggcacg ctgctcgaat acggcggcgt cggcaagatg ggcaagaccg
     5461 agcgcaacgg cgtcgacccg cagg
//
LOCUS       DAOMVO010000158         5447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4124_length_5447_cov_12.377040, whole genome shotgun sequence.
ACCESSION   DAOMVO010000158 DAOMVO010000000
VERSION     DAOMVO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4124_length_5447_cov_12.377040"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..530)
                     /locus_tag="PKV30_08495"
     CDS             complement(279..530)
                     /locus_tag="PKV30_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30287.1"
                     /translation="MARLAAEARHALPERSFAYIDSQGGEHLPIHDAAHVRNAAARFN
                     QTDFESAEAKHEAARHIVAAARRWGVELSDDDTVVAASR"
     gene            626..1342
                     /locus_tag="PKV30_08500"
     CDS             626..1342
                     /locus_tag="PKV30_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRL30288.1"
                     /translation="MTADLPVLYTFRRCPYAMRARWALQAAGVAVAMREIVLRDKPPA
                     MLAASPKGTVPVLVLPGGAVIDESLEVMRWALAQNDPHGWLTPERGTLDDMLALIAAC
                     ERDFKPHLDRSKYASRFRAEWARSGGDEAAEEGAFAARHYAQALQFLELLAHALQAPA
                     AENASEPDAAACLFGTRAALADFAIVPFVRQFARQDMARFAAHAPPPVVQWMDRLLAR
                     PDFEAVMTKRPVGSWRGAPV"
     gene            1394..1750
                     /locus_tag="PKV30_08505"
     CDS             1394..1750
                     /locus_tag="PKV30_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRL30289.1"
                     /translation="MQNILMIVHAPPYGSERVLSALRVALALAGKDSDKPQLNVFLMS
                     DATVLALPNQQDASGNTLQKMLEDLIGHGVPVRLCKTCAGNRGLLDLPLISGVTIGTL
                     AELADATLAADKVITF"
     gene            1779..2921
                     /locus_tag="PKV30_08510"
     CDS             1779..2921
                     /locus_tag="PKV30_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HRL30290.1"
                     /translation="MHALNLYAGPAARRHIERHGLAPAHVGVIPAAAGGPKGLILGPL
                     DRFIFGQWLPQSAQTVNLVGASIGAWRMATACMDDPATHFQALETGYIHGDMTAPPGR
                     KLPLPSQVSATFAQNLRDMFEGHVPTILRHPRYRLHVVTSHGRHVLGREGRLRTAAGY
                     LGAALTNAASRRAMGAWIERVVFSGPAGEGVAPAPLPFDTRDFRTRHVLLTPGNFYPA
                     LQASGSIPFVLQAVHDIPGAPRGAYWDGGITDYHLHLAYNATSSVAAGAGPQSAGGTK
                     DHQAGQIVLYPHFQQAVVPGWLDKPWKRRHGATPFLDHMLVLAPNPEWVRTLPGGKLP
                     DRNDFQKLTHAERVEAWSTSVKAARQLADEFEAWLAQPAAARVQPL"
     gene            3056..3646
                     /locus_tag="PKV30_08515"
     CDS             3056..3646
                     /locus_tag="PKV30_08515"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HRL30291.1"
                     /translation="MIRSRLVLALATAGAALALVGCETYGPVYGTPVGGPVYGSPGYG
                     GPVYGQPGYSQPGYGAGAAFVCESDRGGYRECGVPGGGQAVLVRQLSESACVPGRTWG
                     QRGGAVWVNGGCRGEFAMQGGYGGGAPGYAGGAVVTCNSDEGRTATCGWDPRMGRPVL
                     IEQLSSTPCQEGVTWGQSGRGEIWVSRGCRGRFGVR"
     gene            complement(3677..4697)
                     /locus_tag="PKV30_08520"
                     /pseudo
     CDS             complement(3677..4697)
                     /locus_tag="PKV30_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(4751..>5447)
                     /locus_tag="PKV30_08525"
     CDS             complement(4751..>5447)
                     /locus_tag="PKV30_08525"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRL30292.1"
                     /translation="RGAIVIVDDGRKLGFHSWSLMPKAAICDPALTLGLPPMLTAATG
                     MDAIAHCMEVFMAPAFNPPADGIALEGLRRGWAHIERATRDGSDREARLNMMAASIHG
                     AMGFQKGLGCVHSLSHSLGGANPKLHHGTLNAVFLPAVIRFNAGAESIQKEERLACMA
                     QAMGLEKSADVAQAVHDMTARLGLPTGLAAMGVTEDMFDAVIEHALLDHCHKTNPRIA
                     SADDYRRMLNESM"
BASE COUNT          835 a         1903 c         1865 g          844 t
ORIGIN      
        1 ccttgggccg cgccttgcgt ggcggctcag ggtcaggcgc ctggcgctcg gccgcgcgca
       61 acgcggtggt ggcatgcgcg gcgccggtga tcaggctcat ccagcggacg gtcaactggg
      121 tcagaagctg gtgaatggtt tgtctccttg ttcaccacca gtgcgaacgc ccgcaggcgc
      181 catggttccc gctgattggc gtccaggttt gatcacgcca tgcgcggccc acgtcgcgcg
      241 acgccgtttc gtgtgttgaa aaaatcaagg cccgcgcttc agcgcgatgc ggcaaccacc
      301 gtgtcgtcgt cggacagctc gacaccccat cgcctggccg ccgccacgat gtggcgcgcc
      361 gcctcatgct ttgcttcggc gctctcgaaa tcggtctggt tgaagcgcgc tgccgcgttt
      421 ctgacgtgcg ccgcatcgtg aatcggcagg tgctcgccgc cctggctgtc gatgtaggca
      481 aagctgcgtt cgggcagcgc gtggcgtgct tcggcggcga gtcgggccat ggctttttct
      541 ccagtcgcgc cgaacgacga agaaccgccg accatcagcg caccgctagg atacggccac
      601 gacctgtccg caggggtgac ctgttgtgac cgccgatctg cccgtcctct acacctttcg
      661 ccgctgcccc tacgccatgc gtgcgcgctg ggcgctgcag gcggcgggtg tggcggtggc
      721 catgcgcgag atcgtgctgc gcgacaagcc gcccgccatg ctggccgcct cgcccaaggg
      781 cacggtgccg gtgctggtgc tgccgggcgg cgcggtgatc gacgagagcc tggaggtgat
      841 gcgctgggcg ctggcgcaaa acgaccctca cggatggctg acacccgagc gcggcacgct
      901 ggatgacatg ctggcgctga tcgcggcgtg cgagcgcgac ttcaagccgc atctggaccg
      961 cagcaaatac gccagccgct ttcgcgccga gtgggcacgg tcgggtggcg atgaagcagc
     1021 ggaggagggc gcgttcgcag cgcggcatta cgcccaggcg cttcaatttt tagagctgct
     1081 cgcgcacgcc ctgcaagcgc cagcggccga aaatgcatca gaacccgacg cagccgcttg
     1141 cttgtttggc acgcgcgcag ccctggccga cttcgccatc gtgcccttcg tccgccagtt
     1201 cgcgcggcaa gacatggcgc gcttcgctgc ccacgcgccg ccaccggtgg tgcagtggat
     1261 ggaccgtttg ctggcgcggc ccgacttcga agccgtgatg accaagcgcc cggttgggtc
     1321 gtggcgcggg gcgccagtct gacagctggc ccggctacga ttcaagattc tttgcaacca
     1381 cctgagcaac gccatgcaaa acatactgat gatcgtccac gccccgccgt acggcagcga
     1441 acgcgtgttg tcggccctgc gcgtggcgct ggcgctggcg ggcaaggaca gtgacaagcc
     1501 gcagctcaac gtcttcctga tgtccgacgc caccgtgctt gcgctgccca accagcaaga
     1561 cgcatccggc aacacgctgc agaagatgct ggaagacctc atcggccacg gcgtgccggt
     1621 gcgcctgtgc aagacctgcg ccggcaaccg cggcctgctc gatctgccgc tgatctccgg
     1681 cgtgaccatc ggcacgctgg ccgagctggc cgacgccacg ctggcggccg acaaggtcat
     1741 cacgttctga tggagggacg ctgcggagcc gcgccgccat gcacgccctg aacctgtacg
     1801 ccggccccgc cgcgcgccgc cacatcgagc ggcacggcct ggcgcctgcg cacgtgggcg
     1861 tgattccggc ggcggccggt ggccccaagg gcttaatcct cgggccgctg gatcgcttca
     1921 tcttcggcca atggctgccg caaagcgcgc agaccgtgaa cctggtcggc gccagcatcg
     1981 gcgcctggcg catggccacg gcgtgcatgg acgatccggc cacgcacttc caggcgctgg
     2041 aaacgggcta tatccacggc gacatgaccg cgccgcccgg ccgcaagctg ccgctgccaa
     2101 gccaagtcag cgccacgttc gcgcaaaacc tgcgcgacat gttcgaaggc catgtgccca
     2161 ccatcctgcg ccacccgcgc taccggctgc acgtggtgac ttcgcacggg cgccacgtgc
     2221 tggggcgcga ggggcgcctg cgcaccgccg ccggttacct gggcgcagcg ctcaccaacg
     2281 cggccagccg gcgcgcgatg ggggcgtgga tcgagcgcgt ggtgttttcc ggcccggctg
     2341 gcgagggcgt ggcgccggcg ccgctgcctt tcgacacacg cgacttccgc acgcggcacg
     2401 tgctgctcac gccgggcaac ttctatcccg cgctgcaggc cagcggctcg atccctttcg
     2461 tgctgcaggc ggtgcacgac attccgggcg cgccgcgcgg cgcgtactgg gacggcggca
     2521 tcaccgacta ccacctgcac ctggcctaca acgctacatc ttcagtagct gctggcgctg
     2581 gtccgcaaag cgctggcggc acaaaagacc atcaggccgg gcagatcgtg ctgtacccgc
     2641 atttccagca ggccgtggtg cccggctggc tcgacaaacc ctggaagcgc cgccacggcg
     2701 cgacgccgtt tctggaccac atgctggtgc tggcgcccaa cccggaatgg gtgcgcacgc
     2761 tgccgggcgg caagctgccg gatcgcaacg actttcagaa gttgacgcac gccgagcgcg
     2821 tcgaggcctg gagcacttcg gtgaaggccg cgcggcaact ggcggacgag ttcgaagcct
     2881 ggctggcgca gcctgcggcg gcgcgcgtgc agccgctctg agcgtccggc gatgtcgacg
     2941 gcgtctgaca agcgctcgcg gccttgtctt acaaatgctg cggccaagcg gcccacgcgc
     3001 cacggccgcc gccgcgtagc atcgcccttg tcacgcaatc aaaaaagggc tcaccatgat
     3061 ccgctcccgt ctcgttcttg ccttggccac cgctggcgcc gcgctcgcgc ttgtcggctg
     3121 tgaaacctac ggcccggtct acggtacgcc cgtcggcggg ccggtgtacg gctcgcccgg
     3181 ctacggtggc ccggtctacg gccagcccgg ctattcacaa ccgggttatg gggccggcgc
     3241 tgccttcgtg tgcgagagcg accgcggcgg ctaccgtgaa tgcggcgtgc ccggtggcgg
     3301 ccaggccgtg ctggtgcgcc agttgtccga atcggcctgc gtgccgggcc gcacctgggg
     3361 ccagcgcggc ggcgcggtgt gggtcaacgg cggctgccgc ggcgaattcg cgatgcaggg
     3421 cggttatggc ggcggtgcgc caggctatgc gggcggcgcc gtcgtgacct gcaacagcga
     3481 cgaaggccgc accgccacct gcggctggga tccgcgcatg ggccggccgg tgctgatcga
     3541 gcaactgtcg tccacgccct gtcaggaagg cgtgacctgg ggccagtcgg gtcgtggcga
     3601 gatatgggtc agccgcgggt gtcgtgggcg cttcggcgtg cgttaagcgc caagtagtga
     3661 cggcgtgccg gcgcgcctag ccgtgccggt gctgatggtg cgcatccgga aaatgcggat
     3721 gcgtgtgcgt cagcgctgta tgacgatgcg cgtggccgtg cctcaggccg ggctggactt
     3781 tcccatcgtg cgcatgccgg tggtgttcgt cgtggacatg ctcatgcgtg tgctcaagcg
     3841 cctcgtgcgc gtgttcatgc acgtgccgct cggtcagatg cagccacacg cctgcggcca
     3901 tgcatgcgcc agccagcgcc agcggcacgg tggccggctc gcccagcgcg atggccagca
     3961 acgcgcccag aaaaaggcgc gactgagaag tacgcgccgg tgcgcgccgt gcccagatgg
     4021 cgcaggccca ccacaaacag cgtcaagctg accccgtagg ccagcagccc gaccgccatc
     4081 gcgccggcca catgcggcca cggcggcagc gtggcgccca gcgcaaacgc cagcgccaga
     4141 ttgaccggtg cagccgccag ccccttgacc gaggcgatcc agctggcatc ggccagcgcc
     4201 accttgcgcg tcaggttgtt gtccagggcc cagcacaggc aagcccccag cacggcaagc
     4261 gcgggccaca cgccggcaaa gcgcgcttcg cccggccagc cgaggatgag cgcacccgcg
     4321 acgatcaaga ccatgcccca agccacgcgg cgatccacgt tttcgccgaa cgcaacccac
     4381 gccaacagcg ccgtgaacac gccctcggca ttcagcagca gcgaggcgcc cgatgcgggc
     4441 atgcgtgtca atcccagcat cagcagcacg ggagccgcta cgcccccgca tccgattgcc
     4501 gccgccagcc agggcatttc ggcgcgcgtc agatgcactt tgggcgcacc cgtcagccgc
     4561 cggtacagcc acagccccag gccggagccc aggtaaagca ggccagccag cagccagggg
     4621 ttgaccgaat gcagcaagag cttggccagc ggcgtgccgg cgccaaacag cagcgcagcg
     4681 cccagcgccg ccgccacccc cggctgccga agcgtggtgc gccaacctgg ggcgtctgcg
     4741 cgaagatcgg tcacatcgac tcgttcagca tgcgccgata gtcgtcggcg ctggcgatgc
     4801 gcgggttggt cttgtggcag tggtcgagca gggcgtgctc gatcacggca tcaaacatgt
     4861 cctcggtcac gcccatcgcc gccaggccgg tgggcaggcc caggcgcgcg gtcatgtcgt
     4921 gcacggcctg cgccacgtca gcgctctttt caagccccat cgcttgtgcc atgcacgcca
     4981 agcgctcttc tttttgaatc gattcagccc ccgcgttgaa gcggatcacg gccggcagaa
     5041 acaccgcgtt cagcgtgccg tggtgcagct tggggttggc gccgcccagg ctgtggctca
     5101 agctgtgcac gcagcccagc cctttctgga aacccatggc gccgtgaatg ctggcggcca
     5161 tcatgttcag tcgcgcttcg cggtcgctgc catcgcgcgt ggcgcgctcg atgtgcgccc
     5221 agccgcgccg caggccttcc agcgcgatgc cgtcggccgg cgggttgaag gcgggcgcca
     5281 tgaagacttc catgcagtgc gcgatggcgt ccatgccggt ggcggcggtc agcatgggcg
     5341 gcaggcccag ggtgagggcg gggtcgcaga tggccgcctt gggcatcagc gaccagctgt
     5401 gaaagcccag cttgcgcccg tcatcgacga tgacgatggc gccgcgt
//
LOCUS       DAOMVO010000159         5366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4213_length_5366_cov_12.854830, whole genome shotgun sequence.
ACCESSION   DAOMVO010000159 DAOMVO010000000
VERSION     DAOMVO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4213_length_5366_cov_12.854830"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1459
                     /locus_tag="PKV30_08530"
     CDS             140..1459
                     /locus_tag="PKV30_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HRL30293.1"
                     /translation="MTFTQHPQRALLHNEIHARPPEALAAPLAISHIVMLADEAGRHA
                     SRAHLAELLNGLHQATPEDDASYLRADLGPYTLRWERHTEFVSWTFIRPLTDGDVQAM
                     AQGAAPPCAIDVVSQGWLRALPGENLLSLHLWALPKGALNEMALVRRLLREDTLTGST
                     VISHSSDLFTDLAVHADGFSRMLVFTGPVAPGQVSPRRLGRLVQRLLEIETYRMAALL
                     GLPLAREAARQLAHAELELAQLAQAVRGATREDEPTLLDRLTRLAADLESQYAATHAR
                     FAASAAYDELLNRRLTDIAETRIEGMQSVRDFMQRRLTPAMSTCRSSDRRQAALSQRI
                     ERMSSLLRTRVEVEQQQSSRALLTAMNRRQGMQLKLQSTVEGLSVAAITYYVVGLIGY
                     LAKGARAIGWPFSPEATVAVAVPVVAVAVWWSLRRLHHRIVGHEQDA"
     gene            1536..3146
                     /locus_tag="PKV30_08535"
     CDS             1536..3146
                     /locus_tag="PKV30_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRL30294.1"
                     /translation="MTLSYDNPYRTTRLPVFARNMVATSHPLAAQAGLRMAQQGGNAV
                     DAAVAAAAAMTICEPVSNGLGSDAFCILWDGQQLHGLNAAGRAPAKWSADYFRKKYGA
                     ADAPPKRGIDSVTVPGAVSAWVQLNKRFGRLPLADVLAPAIDIAERGYAVPVVVQQKW
                     AAAVPELESQPGFSQAFMPWGRAPQVGELFRFPAAAQALKRIAASRGVAFYGGEIAHA
                     IQRFAAAQGGVIEASDFERYQPEWVDPLAIGYRGHQLHEIGPSTQGIAALMALGMLEN
                     FDMKALGADRVSAKHVQIETMKLAFADVYRSVAEPSWMAVTPAQMLDAAYLKSRARLI
                     RMNKAQDFGPGLQPQGGTIYLTAADESGMMVSFIQSNYLGFGSGCVEPEFGISLQNRG
                     HGFSLADDSPNVVAPGKRPFHTIIPAFLTKDGQPVMSFGVMGASMQPQGHVQTLVRML
                     DHGQSPQAACDAPRWRVNQGLSLNVEASMDPAIVEGLQARGHRIEAINDSYQDFGAGQ
                     FIWRLGEPGVEGYVGASDARRDGLVAGF"
     gene            3182..4102
                     /locus_tag="PKV30_08540"
     CDS             3182..4102
                     /locus_tag="PKV30_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL30295.1"
                     /translation="MPPALAGQAPAATKSRVSGLVMAALGAICFSGKAIIVKLAYRYG
                     VDAVTLIMYRMLFALPFFVVMAWWAGRGQPPLAQRDWLGVLGLGFIGYYLSSMLDFAG
                     LQYITASLERLILYLGPTLVLLLGWALRGRRITRGQLAATAVSYCGALLVFGIELRVA
                     PTGATAWGALLVFISTFTYALYLFYSGEFVQRLGSLRLVGLATAFACVCCIAQFLLVK
                     PLVAAQVAPEVIWLSVLNATLCTAVPVLLVMMAVERLGPSLASQVGMIGPLSTIAMGI
                     VILGEPFTPWLAAGTALVVAGIFMFSRAGR"
     gene            4136..4933
                     /locus_tag="PKV30_08545"
     CDS             4136..4933
                     /locus_tag="PKV30_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL30296.1"
                     /translation="MDLGIKGKWALVGGASKGLGRGCAEALAGEGVNVLMVARGAEAL
                     AQAAAEIRAACAGGTGAEVQFVAADITTPEGRAAVFAVRRDFDIVVTNAGGPPPGSFR
                     DWDREAWIKAIDANMLTPIELIKATIDGMLARGFGRIVNITSSAVKAPIDILGLSNGA
                     RSGLTGFVAGLARNSEIAAKGVTLNNLLPGKFDTDRLKGTMVGAAQKSGQSVDDVRAA
                     QQKQIPAQRFGTPAEFGAICAFLCSQQAAYLTGQNILLDGGAYPGTF"
     gene            complement(4936..>5366)
                     /locus_tag="PKV30_08550"
     CDS             complement(4936..>5366)
                     /locus_tag="PKV30_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30297.1"
                     /translation="GLFGTGKEMFASGQALMAGAKDYPGNELIQAIVPDPQAADKSAE
                     LDQARKTRDWAMARMKAKGITSAEKLTAQTLEDAREAAQLLDSKVDPAQAAQYRRWAL
                     GVADKVAMASTEGGFLGFGGTRLSDAETSLIDQLKTALRA"
BASE COUNT          816 a         1808 c         1852 g          890 t
ORIGIN      
        1 aagcgcgaag aggccttcgt cgaagagtga cggcgctcgc gcccacggct gcaacggcac
       61 cttcgggtgc cgttgttctt ttggccggcg acagatcgtg ctcgtggaca atgacgctgc
      121 cggccgccat cgcaccacca tgaccttcac ccagcacccg cagcgcgcgc tgctgcacaa
      181 cgagatccac gcccgcccgc ccgaagcgct ggcggcgccg ctggcgattt cgcacatcgt
      241 catgctggcc gacgaggcgg gccgccacgc cagccgcgcg cacctggccg aactgctgaa
      301 cggcctgcac caggccacgc cggaagacga cgccagctac ctgcgtgccg atctgggccc
      361 gtacacgctg cgctgggagc gccacactga attcgtctcc tggaccttca ttcgcccgct
      421 gaccgatggc gatgtgcagg cgatggcgca aggcgctgcg ccgccctgcg ccatcgacgt
      481 cgtgtcgcag ggctggctgc gcgcgctgcc gggcgagaac ctgctcagcc tgcacctgtg
      541 ggctttgccg aagggcgcgc tcaatgagat ggcgctggtg cgccgcctgc tgcgcgagga
      601 cacgctgacc ggctccaccg tcatcagcca cagctccgac ctgttcaccg acctggccgt
      661 gcatgccgac ggcttttcgc gcatgctggt gttcaccggc ccggtggcgc cggggcaggt
      721 gtcgccacgc cggctggggc ggctggtgca gcggctgctg gagatcgaga cctaccgcat
      781 ggccgccttg ctcggcctgc cgctggcgcg cgaggcggcc cgccaactgg cgcacgccga
      841 actggaactg gcgcaactgg cgcaagccgt gcgcggcgcc acacgcgagg acgagccgac
      901 cctgctcgat cgcctgacgc gcctggccgc cgatctggaa agccagtacg cggcgaccca
      961 tgcccgcttt gccgccagcg cggcgtacga cgaactgctc aatcgccgcc tgaccgacat
     1021 cgccgagacg cgcatcgaag gcatgcagtc ggtgcgcgac ttcatgcagc ggcgcctgac
     1081 gccggccatg agcacctgcc gctcgtcgga ccgccggcag gcggcgctgt cgcagcgcat
     1141 cgagcgcatg agcagcctgc tgcgcacccg ggtcgaggtc gagcagcagc agagcagccg
     1201 cgcgctgctg accgcgatga accggcgcca gggcatgcag ctcaagctgc agtccacggt
     1261 ggaagggttg tcggtcgccg ccatcaccta ttacgtggtc ggtttgatcg gctacctggc
     1321 caagggcgcc agggccatcg gctggccgtt cagccccgag gcgacggtgg cggtcgccgt
     1381 gccggtggtc gccgtggccg tctggtggtc gctgcggcgg ctgcaccacc gcatcgtggg
     1441 ccacgagcag gatgcgtagc acgccgccgt gatgctggtc acggtttaca accgtggtca
     1501 tcgcggccgc ggtggctgtg aagaaaatca aacccatgac cctgagttac gacaacccct
     1561 atcgcaccac ccgcctgccg gtgttcgccc gcaacatggt cgccacctcg cacccgctgg
     1621 cggcacaggc ggggctgcgc atggcgcagc agggcggcaa cgcggtcgat gccgccgtgg
     1681 ccgcggcggc cgccatgacg atctgcgaac cggtcagcaa tggcctgggc agcgacgcct
     1741 tctgcatcct gtgggacggc cagcaactgc atggcctgaa cgccgccggc cgtgcgcccg
     1801 ccaagtggag cgcggactac tttcgcaaga aatacggtgc cgccgatgcg ccgcccaagc
     1861 gcggcatcga cagcgtgacg gtgcccggcg ccgtcagcgc gtgggtgcaa ctgaacaagc
     1921 gctttggccg tctgccgctg gccgacgttc tggcgccggc catcgacatc gccgaacgcg
     1981 gctacgccgt gccggtggtg gtgcagcaga aatgggccgc tgccgtgccc gagttggaat
     2041 cgcagccagg cttctcgcaa gccttcatgc cctggggccg cgcgccgcag gtcggtgagc
     2101 tgttccgttt tcccgccgcg gcgcaggcct tgaagcgcat tgccgcctcg cgcggcgtgg
     2161 ccttctacgg cggcgagatc gcgcacgcca tccagcgctt tgccgccgcg caaggcggcg
     2221 tgatcgaagc cagcgacttc gagcgctatc agcccgaatg ggtcgatccg ctggccatcg
     2281 gctaccgcgg ccaccagctg cacgagatcg ggcccagcac gcaaggcatc gcggcgctga
     2341 tggcgctcgg catgctggaa aacttcgaca tgaaggcgct gggcgccgac cgcgtgagcg
     2401 ccaagcacgt gcagatcgag accatgaagc tggcctttgc cgacgtttac cggtccgtgg
     2461 ccgagccttc gtggatggcg gtgacccccg cgcaaatgct cgatgccgcg tacctgaagt
     2521 cgcgtgccag actcatccgc atgaacaagg cgcaggattt cgggcccggc ctgcaaccgc
     2581 agggcggcac catctacctg acggcggcgg acgagagcgg catgatggtc agcttcatcc
     2641 agagcaacta cctgggcttt ggctcgggct gcgtcgagcc ggaattcggc atcagcctgc
     2701 aaaaccgcgg ccacggcttc agcctggctg acgacagccc gaacgtggtg gcgcccggca
     2761 agcggccgtt tcacaccatc attcccgcgt tcttgaccaa ggacggccag cccgtgatga
     2821 gctttggcgt gatgggcgcg agcatgcagc cgcagggcca cgtacagacg ctggtgcgca
     2881 tgctcgacca cggccagagc ccgcaggccg cgtgcgatgc gccgcgctgg cgcgtcaacc
     2941 aaggcttgtc gctcaacgtc gaggcgagca tggacccggc catcgtggaa ggtctgcagg
     3001 cgcgcggcca ccgcattgag gccatcaacg acagctatca ggatttcggc gccggccagt
     3061 tcatctggcg cctgggcgag ccgggcgttg aaggctacgt gggcgccagc gatgcgcggc
     3121 gcgatgggct ggtggcgggc ttctgaaatc cgtcgtgacc accgccgata tcaagcaaaa
     3181 aatgcctcca gcgcttgctg gacaagcgcc agcagctaca aaaagcagag tatccgggct
     3241 ggtgatggcg gcactcggcg ccatctgttt cagcggcaag gccatcatcg tcaagctggc
     3301 ctaccgctac ggcgtcgatg cggtcacgct gatcatgtac cgcatgctgt ttgcgctgcc
     3361 gttcttcgtc gtcatggcgt ggtgggccgg gcgcggccag ccgccgctgg cgcagcgcga
     3421 ctggctcggc gtgctgggcc tgggtttcat cggctactac ctgtcgagca tgcttgactt
     3481 tgcgggcctg caatacatca ccgccagcct ggagcgattg atcttgtacc tgggcccgac
     3541 gttggtgctg ctgctcggct gggcgctgcg cgggcggcgc atcacgcggg ggcaactggc
     3601 ggccaccgcc gtcagctact gcggtgcgct gctggtcttt ggcatcgagc tgcgcgtggc
     3661 gcccaccggg gccacggcct ggggtgcgtt gctggtgttc atctccacct tcacctacgc
     3721 gctttatctt ttctacagcg gcgaattcgt gcaacgcctg ggctcgctgc ggctggtggg
     3781 gctggcgacg gcgtttgcct gcgtgtgctg catcgcgcag ttcctgctcg tcaagccgct
     3841 ggtcgcggcg caggtcgcgc ccgaggtgat ctggctgtcg gtactcaacg ccacgctgtg
     3901 cactgcggtg ccggtgctgc tggtgatgat ggcggtcgag cgcctggggc cttcgctggc
     3961 ctcgcaggtc ggcatgatcg gcccgctgtc gaccatcgcc atgggcatcg tgatcctggg
     4021 cgagcccttc acgccgtggc tggcggcggg cacggcgctg gtggtggcgg gcatcttcat
     4081 gttcagccgg gcagggcgct agagtcttgc tgttcaacaa gcaaggagca atccgatgga
     4141 tcttggtatc aagggcaaat gggcgctggt gggtggcgcc agcaaagggc tggggcgcgg
     4201 ctgcgccgag gcgctggcgg gcgagggcgt gaatgtgctg atggtggcgc gcggcgctga
     4261 agcgctggcc caggctgctg ccgaaataag agctgcctgc gcaggtggga cgggcgctga
     4321 ggtgcaattt gtggccgcag acatcaccac gcccgaaggc cgcgcggcgg tgtttgccgt
     4381 gcggcgcgac ttcgacatcg tggtcaccaa cgcgggcggg ccgccgcccg gcagctttcg
     4441 cgactgggac cgcgaggcct ggatcaaggc catcgacgcc aacatgctga cgcccatcga
     4501 gctgatcaag gcgaccatcg acggcatgct ggcgcgcggc tttgggcgca tcgttaacat
     4561 cacgtcgagc gcggtgaagg cgccgatcga catcctgggt ttgtcgaacg gtgcgcgatc
     4621 cggcctcacc ggcttcgtcg ccggcctggc gcgcaactcc gagatcgccg ccaagggcgt
     4681 gaccctcaac aacctgctgc caggcaagtt cgacaccgac cggctcaagg gcaccatggt
     4741 cggcgcagcg cagaaaagcg gccagtcggt cgacgacgtt cgggctgcgc agcaaaaaca
     4801 gattccggcg cagcgctttg gcacgccggc cgaattcggc gccatctgcg cctttctgtg
     4861 cagccagcag gcggcctacc tgacggggca gaacatcctg ctggacggcg gcgcctatcc
     4921 cggcacgttc tgagttcagg cgcgcagcgc ggtcttgagc tggtcgatca gcgacgtttc
     4981 ggcgtcgctc aggcgggtgc cgccaaagcc gagaaagccg ccttcggtcg aggccatggc
     5041 caccttgtcg gccacgccca gcgcccatcg ccgatactgc gcggcctgtg ccgggtcgac
     5101 cttgctgtcc agcaactgcg ccgcttcgcg tgcgtcttcc agcgtttgcg cggtgagttt
     5161 ttcggccgag gtgatgccct tggccttcat gcgcgccatg gcccaatcgc gcgtcttgcg
     5221 cgcttggtcg agttcggcgc ttttgtcggc ggcctgcggg tcgggcacga tggcctggat
     5281 cagctcgttg ccggggtagt ccttggcgcc cgccatcagc gcctggccgc tggcgaacat
     5341 ctccttgccg gtgccgaaca ggccgc
//
LOCUS       DAOMVO010000160         5304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4299_length_5304_cov_14.178891, whole genome shotgun sequence.
ACCESSION   DAOMVO010000160 DAOMVO010000000
VERSION     DAOMVO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_5304_cov_14.178891"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKV30_08555"
     CDS             <1..495
                     /locus_tag="PKV30_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL30298.1"
                     /translation="GSSGHLVMAALENQTGMRINHVPYKGMQQILGDLMNGNVKIGVV
                     DVSSSLALIKAGKIRPLAVSGSARMPASRDVPTMTEQGYRFDTDGWFALFAPKNTRPA
                     VVSVLNREVADALAAPELKARLLQLNLANAPRKTPKQFAQTVRDDLKVWTKIAQDNKI
                     TPDD"
     gene            507..1196
                     /locus_tag="PKV30_08560"
     CDS             507..1196
                     /locus_tag="PKV30_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRL30299.1"
                     /translation="MIARTMGAAGFGARSIAPGLMICLTIALAATFLSEHYGGPQFLY
                     ALLFGMSLNFLTPSDSARTVLRLGVALLTQVLSRDATAAGIFLGATIHDVAQVVGAGY
                     MISPEAGDMATVVKLLRVAMLLPVVALVGIYFGRREGGQSSSLLATVPWFLLVVAALV
                     IVISLHWVPPPLITAMQDTSRWCLVVAITGLGVKTSIQQLAQVGWRPIAVLLANTVFL
                     AVFAGALLARA"
     gene            complement(1198..1761)
                     /gene="phaR"
                     /locus_tag="PKV30_08565"
     CDS             complement(1198..1761)
                     /gene="phaR"
                     /locus_tag="PKV30_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRL30300.1"
                     /translation="MSPSKPTAPAKKKAPTAAEADSARVIKKYPNRRLYDTQHSSYIT
                     LTQVRELVMAHTPFVVRDAKTGEDLTRSILLQIILEQEAGGAPMFTEAMLAHMIRFYG
                     HAMQGFMGNYLEKNIQTFTEIQSRLSEQSQSLTPEMWSQFMRMQSPMMQGMMGNYLEQ
                     SKNMFLQMQEQMAKQAEQVLGAFTGKR"
     gene            complement(1858..2514)
                     /gene="ureG"
                     /locus_tag="PKV30_08570"
     CDS             complement(1858..2514)
                     /gene="ureG"
                     /locus_tag="PKV30_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HRL30301.1"
                     /translation="MTALHHIPNRTKKLPPLRVGIGGPVGSGKTTLLEMLCKTMRDRY
                     DLIAITNDIYTKEDQRLLTVSGALPAERIMGVETGGCPHTAIREDASINLEAIDRMLA
                     QFPDADIVFVESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGITKSDVFVIN
                     KTDLAPHVGASLDVMRADTERMRTTAQGRKPFFMTNLKTGAGLREVVDCIEARGLLNP
                     "
     gene            complement(2585..3289)
                     /locus_tag="PKV30_08575"
     CDS             complement(2585..3289)
                     /locus_tag="PKV30_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HRL30302.1"
                     /translation="MTAPAPLAAASLLQLIWLASPALPVGGFSYSEGLEAGVEWAGVT
                     TENAASDWISDQLHVSQARSDLPVLAQAIVAWRVGDLDRVRALNDWVLATRETSELRL
                     QTEQMGKSLADWLRNLNADRPEHLAAVDRLAALPPTYPIAFSLAVAHTGASVRDGLLA
                     SAFGWAENMVQAALKAVPLGQSAGQRILARLAGEIPAAVDAALACGDAQRQSFSPMLA
                     ILSARHEAQYSRLFRS"
     gene            complement(3286..3807)
                     /gene="ureE"
                     /locus_tag="PKV30_08580"
     CDS             complement(3286..3807)
                     /gene="ureE"
                     /locus_tag="PKV30_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HRL30303.1"
                     /translation="MLTLHKLIPQGRGLAPVLVKRAASVELDWDVRQKSRLGATDSQG
                     RAIGVFLPRGTLLRGGDVLVAEDGSLIRVIAAPQAVLRITPCAVHGSPFDLTRAAYHL
                     GNRHVPIELTPEHLQIEPDHVLADMLRAMHLTVVELQAPFEPEGGAYAAHGHGHHQAH
                     HDHGHGHDHAHHP"
     gene            3876..4142
                     /locus_tag="PKV30_08585"
     CDS             3876..4142
                     /locus_tag="PKV30_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRL30304.1"
                     /translation="MNAKPQTSASSPAAATVLKITQIGNSVGVILPKDVLARLKVGKG
                     DSLFVTEVPDGVTLRPYDDTFEHQMALARDIMKEYREVLRELAK"
     gene            4139..4540
                     /locus_tag="PKV30_08590"
     CDS             4139..4540
                     /locus_tag="PKV30_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRL30305.1"
                     /translation="MTQAANEPWVWVPLALILAVHDEQLTDHGGLQGVRDEGLLVSAL
                     ARAQHLASYGAPDVAQLAAAYAFGIARNHPFADGNKRAAFVAAQLFLRLNGHRINAPL
                     VDCVLTMLQLAAGDMDEDAYAAWLRRHLQAL"
     gene            complement(4576..>5304)
                     /locus_tag="PKV30_08595"
     CDS             complement(4576..>5304)
                     /locus_tag="PKV30_08595"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HRL30306.1"
                     /translation="IAEDLAFAESRIRKETIAAEDVLHDLGAISIMSSDSQAMGRVGE
                     VILRTWQTAHKMKAQRGALADDTARHDNFRAKRYVAKYTINPAITHGIAHEVGSVEVG
                     KWADLVLWKPAFFGVKPALILKGGSIAMAAMGDPNASIPTPQPVHYRPMFASYGGSRT
                     RSSLTFVSQAALAAGVGERFGLHKPLVAVKNTRRIGKRDMVHNAYAPHMEVDAQTYAV
                     RADGQLLTCEPAHSLPMAQRYFLF"
BASE COUNT          831 a         1714 c         1783 g          976 t
ORIGIN      
        1 ggctcgtcgg gccatctcgt catggcggcg ctggagaacc agaccggcat gcgcatcaac
       61 catgtgccct acaaaggcat gcagcagata ctgggcgacc tgatgaacgg caacgtcaag
      121 atcggcgtcg tcgatgtgtc gtcgtcgctg gcgctcatca aggccggcaa gatcaggccc
      181 ctggccgtga gcgggtcggc gcgcatgccc gcgtcgcgcg acgtgccgac catgaccgag
      241 caaggctatc gcttcgacac cgatggctgg tttgcgctgt tcgcccccaa gaacacgcgg
      301 cccgccgtcg tgtccgtgct caatcgcgag gtggccgatg cgctggcagc gcccgagttg
      361 aaggcgcgcc ttttgcaact gaacctggcc aacgcaccgc gcaagacgcc caagcagttt
      421 gcgcaaaccg tgcgtgacga cctgaaggtg tggaccaaga tcgcccagga caacaagatc
      481 acgcccgacg attgagaagg caccccatga ttgcgcgcac gatgggcgct gccggattcg
      541 gcgcgcgcag catcgccccg gggctgatga tctgcctgac catcgccctg gcggccacct
      601 tcctgtccga acactacggc ggcccacagt ttctgtacgc gctgctgttc ggcatgagcc
      661 tgaacttcct gacgccatcg gattcggcgc gcaccgtatt gcgcctgggc gtcgcgctcc
      721 tgacgcaagt gctcagccgg gacgcgacgg ccgcaggcat cttcctgggt gccaccattc
      781 acgacgtggc gcaggtggtc ggcgcgggct acatgatctc gcctgaggcg ggtgatatgg
      841 ccaccgtggt caagctgctg cgcgtggcga tgctgttgcc ggttgtggcg ctggtcggca
      901 tctatttcgg ccggcgcgaa ggcggccagt cgtccagcct gctggccacc gtgccctggt
      961 ttctgctggt ggttgccgcg ctggtcatcg tgatcagcct gcactgggtg ccgccgccgc
     1021 tcatcaccgc catgcaggac acgtcccgct ggtgcctggt cgtcgccatt accggactcg
     1081 gcgtgaaaac ctcgattcag cagctggcgc aggtgggctg gcggccgatc gcggtgctgc
     1141 tggcgaacac ggtgtttctg gcggtctttg cgggcgccct gctggcgcgc gcctgaatca
     1201 ccgcttgccg gtgaacgcgc ccagcacctg ctcggcctgc ttggccattt gctcctgcat
     1261 ctgcaggaac atgttcttcg actgctccag atagttgccc atcatccctt gcatcatggg
     1321 cgactgcatg cgcatgaact ggctccacat ctcgggcgtc aggctttgcg actgctcgga
     1381 caggcgtgac tggatctcgg tgaaggtctg gatgttcttt tccagatagt tgcccatgaa
     1441 gccctgcatg gcgtggccgt agaagcggat catgtgggcc agcatggcct cggtgaacat
     1501 cggcgcgcca ccggcctctt gttccagaat gatctgcagc aggatgctgc gcgtcaggtc
     1561 ttcacccgtc ttggcgtcgc gcaccacgaa aggcgtgtgc gccatcacca actcgcgcac
     1621 ctgcgtcagc gtgatgtagc ttgaatgctg ggtgtcgtaa agacggcggt tcgggtattt
     1681 cttgatgacg cgcgccgagt cggcttcagc ggcggtgggc gccttctttt tcgccggcgc
     1741 ggtgggtttg cttggactca tgacggcggg ccttcggaat gctgctgcac tgcaacagat
     1801 tgtaggcggc gccgacggcc agcccgcgtc aggctttccc caacaggctc cgtcgcgtca
     1861 ggggttgagc agcccgcgcg cctcgatgca atcgaccact tcgcgcaggc cggcgccggt
     1921 tttcaggttg gtcatgaaaa agggcttgcg tccttgcgcc gtggtgcgca tgcgttcggt
     1981 gtcggcgcgc atcacgtcca ggctggcgcc cacgtgcggc gccagatccg ttttattgat
     2041 gacgaacacg tcgctcttcg tgatgccggg cccgcccttg cgcggaatct tctcgcccgc
     2101 ggccacgtcg atgacgtaga tcgtcaaatc gctcagttcg gggctgaagg tggcggccag
     2161 gttgtcgccg ccgctttcga caaacacgat gtcggcgtcg ggaaactgcg ccagcatgcg
     2221 gtcgatggcc tcaaggttga tggacgcatc ttcgcgaatc gccgtgtgcg ggcagccgcc
     2281 ggtttccacg cccatgatgc gctcggccgg cagcgcgccg ctcaccgtca gcaggcgctg
     2341 gtcttccttg gtgtagatgt cgttggtgat ggcgatcagg tcgtagcgat cgcgcatggt
     2401 tttgcagagc atctccagca aggtggtctt gccactgccg accgggccgc caatgcccac
     2461 gcgcagcggt ggcagcttct tggttcggtt cgggatgtgg tgcagtgcgg tcatggtggt
     2521 ttatgaataa aaaatgccgc cagcgcttta tggatcagcg caagcagcta tcaaaaaaat
     2581 agtttcatga tcggaacagg cgcgagtact gcgcctcgtg ccgcgccgac agaatcgcca
     2641 gcatggggct gaaggactgg cgctgcgcgt cgccgcaggc cagggcggca tcgacggcgg
     2701 cggggatttc gcccgccagc cgcgccagga tgcgctggcc cgcgctctga cccagcggca
     2761 cggccttgag cgccgcctgc accatgttct cggcccagcc gaaggcgctg gccagcaggc
     2821 cgtcgcgcac gctggcgccc gtgtgcgcca ccgcgagcga gaaggcgatc gggtacgtgg
     2881 gcggcagcgc tgcgagccga tccacggcgg ccagatgctc gggccggtcg gcgttcaggt
     2941 ttcgcagcca gtcggccagc gacttgccca tctgctcggt ctgcaggcgc aactcgctgg
     3001 tttcgcgcgt ggcaagcacc cagtcgttca gcgcgcgaac acggtccaag tccccaactc
     3061 gccaggccac gatggcctgc gccagcacgg gcaggtcgct gcgcgcctgg ctcacgtgca
     3121 gttggtcgga gatccagtcg gacgctgcgt tttctgtagt gacgcccgcc cattcgacgc
     3181 ccgcctccag cccctcggag taagaaaagc cacccaccgg cagcgccggc gaagcgagcc
     3241 aaatgagctg cagcaggctg gcggcggcca gcggcgcggg ggccgtcatg ggtggtgggc
     3301 gtggtcgtga ccatggccgt gatcgtggtg cgcctggtgg tgcccgtggc catgcgccgc
     3361 atacgcgccg ccctcgggct cgaacggcgc ctgcagctcg actaccgtca ggtgcatggc
     3421 gcgcagcatg tcggccagca cgtggtcggg ctcgatctgc agatgctcgg gcgtgagttc
     3481 gatcggcacg tgccggttgc ccaggtgata ggccgcgcgc gtcaagtcga agggcgagcc
     3541 gtgcaccgcg cacggcgtga tgcgcagcac ggcctgcggc gcggcgatga cgcggatgag
     3601 cgagccgtct tcggccacca gcacgtcgcc gccgcgcagc agcgtgccgc gcggcaaaaa
     3661 tacgccgatc gcacgcccct ggctgtcggt ggcgcccaag cggcttttct ggcgcacgtc
     3721 ccagtcgagt tcgacactgg ccgcgcgctt gaccagcacc ggcgccaggc cccggccttg
     3781 cgggatgagt ttgtggaggg tgagcatggc ttgcctgaaa cgggtgtcgc gattaatata
     3841 acgaccgtta taactcgatt gcttcggagt ccgccatgaa cgccaagccc cagaccagtg
     3901 cttcgtcccc cgccgccgcc accgtcctca agatcaccca gatcggcaac tcggtcggcg
     3961 tgatcctgcc caaggacgtg ctggcgcggc tgaaagtggg caagggggat tcactgtttg
     4021 tcaccgaggt gccggatggg gtgacgctgc ggccgtatga cgacacgttc gaacaccaga
     4081 tggcgctggc gcgcgacatc atgaaagagt atcgcgaagt gctgcgcgag ctggccaaat
     4141 gacccaagcc gcgaacgagc cttgggtgtg ggtgccgctg gccctgattc tggcggtgca
     4201 cgacgagcag ttgaccgacc acggcggctt gcagggcgtg cgcgatgaag gcctgctggt
     4261 ctcggccctc gcgcgggcgc aacacctggc ctcttatggc gcgcccgacg tggcccagct
     4321 ggcggcggcc tatgcgttcg gcattgcgcg caaccacccg tttgccgatg gcaacaagcg
     4381 ggctgccttc gtggcggcgc aacttttttt gcggctcaac gggcaccgca tcaacgcgcc
     4441 gctggtggac tgcgtgctga ccatgctcca actggccgcc ggcgacatgg acgaagacgc
     4501 ctatgccgcc tggctgcggc ggcatttgca ggcgctttga agattgcgcg gcacgcacca
     4561 cagcatccat ccgatctaga acaaaaagta gcgctgcgcc atcggcaggc tgtgcgccgg
     4621 ttcgcaggtc agcagttgcc catcggcacg cacggcgtag gtttgcgcat cgacctccat
     4681 gtgcggcgca taggcgttgt gcaccatgtc gcgcttgccg atgcggcgcg tgttcttcac
     4741 cgccaccaac ggcttgtgca ggccaaagcg ctcgcccacg ccggcagcca gcgcggcctg
     4801 cgacacgaag gtgaggctgc tgcgcgtgcg cgatccgcca tacgacgcaa acatgggccg
     4861 gtagtgcacc ggctgcggcg tgggaatcga tgcgttcggg tcgcccatcg ccgccatggc
     4921 gatgctgccg cccttgagga tgagcgcggg cttgacgccg aagaaggcgg gcttccacag
     4981 caccaggtcg gcccacttgc cgacttcgac gctgcccacc tcatgcgcga tgccgtgggt
     5041 gatcgccggg ttgatcgtgt acttggcgac gtagcgcttg gcacggaagt tgtcgtggcg
     5101 cgccgtgtcg tccgccaggg cgccgcgctg ggccttcatc ttgtgcgccg tctgccaggt
     5161 gcgcaggatc acctcgccga cgcggcccat ggcctggctg tcgctgctca tgatgctgat
     5221 ggcgcccagg tcgtgcagca cgtcttcggc ggcaatcgtc tccttgcgga tgcggctttc
     5281 ggcaaaggcc aggtcttcgg caat
//
LOCUS       DAOMVO010000161         5286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4318_length_5286_cov_13.784554, whole genome shotgun sequence.
ACCESSION   DAOMVO010000161 DAOMVO010000000
VERSION     DAOMVO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4318_length_5286_cov_13.784554"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKV30_08600"
     CDS             <1..233
                     /locus_tag="PKV30_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRL30307.1"
                     /translation="FAIAILAEAALSYLGLGTQPPQPSWGRMLAEAQTLMFQAPLLAV
                     WPGVAIALSVLGLNLLGDGLRDVLDPRLQRRG"
     gene            400..663
                     /locus_tag="PKV30_08605"
     CDS             400..663
                     /locus_tag="PKV30_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRL30308.1"
                     /translation="MTTTVKLPPELEQSLRQRCAAEGRSISDVMRDALLAYLASAPTG
                     TASAWSLGADLFGRHAGPADLATARRQHLGDAWGDKHARRSAH"
     gene            683..1168
                     /locus_tag="PKV30_08610"
     CDS             683..1168
                     /locus_tag="PKV30_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL30309.1"
                     /translation="MSESAPAPQVAEAPAQYGVVPQGQVLVDSGPLLALFNGADHWHA
                     RVVAWLQAHPALALVSTWPVLTEVCALLARRVHNQAALDFLLWVERGALQVDAPQDGS
                     LAQMRRIAQRFASPPLDLAYASIAEAAERLGIRRIVSIDADFDVYRDTRGKPLTNLLR
                     A"
     gene            1168..2178
                     /locus_tag="PKV30_08615"
     CDS             1168..2178
                     /locus_tag="PKV30_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30310.1"
                     /translation="MPLLEARDLRVDLHTHRGTAAAVQGMSFTLEPGDTLGLIGESGC
                     GKSMTALALMGLAPDNATVSGSLKLEGRELIGLRDRDWRAIRGFRLAMIFQEPMTALN
                     PVHRVGAQIAEPLMLHQNLSHGAAREKAIALLERVGIPDPARRIDSFPHQFSGGQRQR
                     IMIAMALACEPDVLIADEPTTALDVTVQKQILALIRELVDERGMVLILISHDLGLIAQ
                     NVQRMMVMYGGAVVESGPAAQVFGQPAHPYTRGLLAARPSLAQRLVAHRSERLPTIKG
                     SVPDLFSLPLGCPFAGRCAYTEDACFADEPPVVPMGSAHWFRCRRADVVLDTPELIAG
                     SA"
     gene            2184..2996
                     /locus_tag="PKV30_08620"
     CDS             2184..2996
                     /locus_tag="PKV30_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30311.1"
                     /translation="MRQSPFLLEIRDVTRRYTLPRESLFKPPAEVQALRGVSLNVTVG
                     RSMGIVGESGSGKSTLARLVMALEAPSSGSICFEGRDLNALSANELRAARRDFQMVFQ
                     DPYGSLDPRRPVWRTVAEPAAALEGAKRAQQRERAAATLESVGLRADALDKFPHEFSG
                     GQRQRIAIARALITKPKLIVADEAVSALDVSVQAQVLNLMADLRDEHGVTFLFISHDL
                     AVVSHLCADVAVMKGGLIVEQGPVRQVLREPTHEYTRTLLESVPELVAPQAA"
     gene            3060..4547
                     /locus_tag="PKV30_08625"
     CDS             3060..4547
                     /locus_tag="PKV30_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30312.1"
                     /translation="MKRRDFALTLPALAALAHAPAFAQSRKDAVVMGMTLEPPGLDPT
                     AAAASAIAEVTQYNIFETLTKINADGSVTPLLAERWEVSPDLKTYTFHLRRGVKFSNG
                     EPFNAATVKYAFERAGGEKSTNKDKRTFAAMESVRPIDDHTVVIINKEPDPDFLFYMG
                     QATASMVEPKSADGNATKPVGTGPYVLDNWSKGSSIVLKASPSYRNPGAIKIKRATFR
                     FISDPAAQVAAVLSGDVDAFVRVTPRGAAQFKANPRLQTITSGSRAKTVLAINNARKP
                     LNDVRVRRAISMAIDRKAVIAGAGDGFGVPIGSHYVPGAAGYIDTTGVNPYDIEKAKA
                     LLKEAGVTTPLELTLVLPPPPYARQGGEVIVAQLAKIGIVAKVQNVEWAQWLANTYGG
                     PKNYDLTIISHVEPFDLANFTKADYYWGYNNPKFNALYDKIKTTPNVAERNKLLGEAQ
                     KMLADDAVLAWLYQPQWVTVAKRGLNGLWKDMPIFVNDLAALSWT"
     gene            4622..>5286
                     /locus_tag="PKV30_08630"
     CDS             4622..>5286
                     /locus_tag="PKV30_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRL30313.1"
                     /translation="MKTALHDLSAHDLLAGYKARDFSPVEVTQAVLAQIERWEPQLAA
                     TYLLRPEAALAQARASEARWLKGEPCGTLDGVPVTIKDNIATQGDPVPLGTKAVALTP
                     AAADAPPAARLRESGAVIVCKTTMPDYGMLSSGLSTFHKTARNPWDVTRTPGGSSAGA
                     GAAAAAGYGPLHIGTDIGGSIRLPAAWCGVVGLKPSLGRVPIDPPYMGRAAGPMTRSV
                     ADAA"
BASE COUNT          883 a         1869 c         1732 g          802 t
ORIGIN      
        1 gctttgccat cgccatcctg gccgaggccg cgctgtcgta cctgggcctg ggcacgcagc
       61 cgccgcagcc cagctggggc cgcatgctgg ccgaggcgca gacgctgatg ttccaggctc
      121 cgctgctggc cgtgtggccg ggcgtggcga tcgcgttgtc ggtgctgggg ctcaacctgc
      181 tgggtgatgg gctgcgcgat gtgcttgatc cgcggctgca acggcgcggg tgatccatga
      241 cgaaatgact tcgccgctgc gcgacatgaa tgacccaatg acccagcccc gttcatgtcg
      301 cgaagcgccg tcatcgatgc gaagcgaggt aatttcgtca tcgcttgcaa atttcaggca
      361 cgcaggtaat atattgttta ttaccaaaag gacgacgcca tgaccaccac cgtcaagctc
      421 ccgcctgaac tggagcaatc gctgcgccag cgctgcgcgg ccgagggccg cagcatcagc
      481 gacgtgatgc gcgacgcgtt gctggcgtat ctggcgagcg cgccgaccgg caccgcgtcg
      541 gcctggtcgc tgggtgccga cctgttcggc cgccacgccg gcccggccga tctggccacc
      601 gcgcgccgcc agcatctggg cgacgcttgg ggcgacaagc acgcccgccg cagcgcgcat
      661 tgaagcttct tggccgtcaa cgatgagcga aagcgccccc gccccgcaag ttgccgaagc
      721 gcccgcgcaa tacggcgtgg tgccgcaggg ccaggtgctg gtcgacagtg gcccgctgct
      781 ggcgctgttc aacggcgccg accactggca cgcgcgcgtc gtggcgtggc tgcaggcgca
      841 ccctgccctg gcgctggtca gcacctggcc ggtgttgacc gaggtgtgcg cgctgctggc
      901 gcggcgtgtg cacaaccagg ccgcgctgga ctttctgctg tgggtcgagc gcggtgcgtt
      961 gcaggtcgat gcgccgcaag acggcagcct ggcgcagatg cgccgcatcg cgcagcgctt
     1021 tgccagcccg ccgctcgatc tggcctacgc gtcgattgcc gaagccgccg agcgcttggg
     1081 cattcgccgc atcgtcagca tcgacgccga tttcgacgtc taccgcgaca cacgcggcaa
     1141 gccgctcacc aacctgctgc gcgcctgatg ccgctgctcg aagcccgcga cctgcgcgtc
     1201 gatctgcaca cccaccgcgg cacggcggcg gcggtgcagg gcatgtcgtt cacgctggag
     1261 ccaggcgaca cgctgggcct gatcggcgaa tccggctgcg gcaagtcgat gaccgcgctc
     1321 gcgctgatgg gtctggcgcc cgacaacgcc accgtcagcg gcagcctgaa gctggaaggg
     1381 cgcgagctga tcgggttgcg cgaccgcgac tggcgcgcca ttcgcggctt ccggctggcg
     1441 atgatttttc aggagccgat gaccgcgctc aaccccgtgc accgcgtagg cgcgcagatt
     1501 gccgagccgc tgatgctgca ccaaaacctg tcgcacggcg ccgcacgcga aaaagccatc
     1561 gccctgctgg agcgcgttgg catccccgat cccgcgcggc gcatcgacag ctttccgcac
     1621 cagttctcag gcggccagcg ccagcgcatc atgatcgcca tggcgctggc ctgcgagccc
     1681 gacgtgctga tcgcagacga gccgaccacc gcgctcgacg tcacggtgca aaagcaaatc
     1741 ttggcgctga tccgcgaact ggtggacgag cgcggcatgg tgctcatcct catctcgcac
     1801 gaccttggcc tgatcgcgca gaacgtgcag cgcatgatgg tgatgtacgg cggcgcggtg
     1861 gtggaaagcg gccccgcggc gcaagtcttt ggccagcccg cgcaccccta cacgcgcggt
     1921 ctgctggccg cgcgcccgag tctggcccag cgcctggtcg cgcaccgcag cgagcggctg
     1981 cccaccatca aggggagcgt gcccgacctg ttcagcctgc cgctcggctg cccgtttgcc
     2041 gggcgatgcg cgtacaccga agacgcgtgc ttcgccgacg agccgccggt agtacccatg
     2101 ggcagcgcgc actggttccg ctgccggcgt gccgacgtgg tgctggatac tcccgaatta
     2161 atagctggta gcgcttgatg gacgtgcgcc agagcccttt tctgcttgaa atccgcgacg
     2221 tgacgcggcg ctacacgctg ccgcgcgaat cgttgttcaa gccgcccgcc gaagtgcagg
     2281 cgctgcgcgg cgtatcgctc aacgtcacgg tcggccgcag catgggcatc gtgggtgaat
     2341 cgggctccgg caagagcacg ctggcgcggc tggtaatggc actggaggcc cccagcagcg
     2401 gcagcatctg cttcgaggga cgcgacctga acgcgctctc agccaacgaa ctgcgcgcgg
     2461 cgcggcgcga tttccagatg gtgtttcaag acccgtatgg ctcgctcgac ccgcgccgcc
     2521 ccgtctggcg caccgtcgcc gaacccgccg cggcgctcga gggcgccaag cgcgcccagc
     2581 agcgcgagcg cgccgccgcc acgctcgaat cggtgggctt gcgcgccgat gcgctcgaca
     2641 agtttccgca tgaattctca ggcggccagc gccagcgcat cgccattgcc cgggcgctga
     2701 tcaccaaacc caagctcatc gtcgctgacg aggcggtgag cgcgctcgat gtctcggtgc
     2761 aggcgcaggt gctcaacctg atggccgatc tgcgcgacga gcatggcgtg acctttctct
     2821 tcatcagcca cgacctggcc gtggtcagcc acctgtgcgc cgacgtggcg gtgatgaagg
     2881 gcggcctgat cgtcgagcaa ggccccgtgc gccaggtgct gcgcgagccg acgcacgagt
     2941 acacgcgcac cctgcttgaa tcggtgcccg agcttgtcgc cccgcaggcg gcttagcgcg
     3001 ttaggggcct gcgctaccat gcgcagcgtc cgacccttct cagccggaga gccctcacga
     3061 tgaaacgccg cgacttcgcc ctcaccctgc ccgcgctggc cgcgttggcc cacgcccccg
     3121 cgttcgcgca gtcgcgcaag gacgcggtcg tgatgggcat gacgctggag ccgccggggc
     3181 tcgacccgac ggccgccgcc gcgtcggcga ttgccgaagt cacgcagtac aacatcttcg
     3241 agacgctgac caagatcaac gccgatggca gcgtcacgcc gctgctggcc gagcgctggg
     3301 aagtctcgcc cgatctgaag acctacacct tccacctgcg ccgcggcgtc aagttcagca
     3361 acggcgagcc gttcaatgct gccacggtga agtacgcctt cgagcgtgcc ggcggcgaga
     3421 aaagcaccaa caaggacaag cgcacctttg ccgccatgga aagcgtgcgg cccatcgacg
     3481 accacaccgt ggtcatcatc aacaaggagc cggaccccga cttcctgttc tacatgggcc
     3541 aggcgacggc cagcatggtc gagcctaaaa gcgccgacgg caacgccacc aagcccgtcg
     3601 gcaccggccc ctacgtgctc gacaactggt cgaagggctc gtccatcgtg ctgaaggcct
     3661 cgcccagcta ccgcaatccg ggcgccatca agatcaagcg cgccaccttc cgcttcatca
     3721 gcgatccagc ggcgcaggtg gcggccgtgc tgtcgggcga cgtggatgcc ttcgtgcgcg
     3781 tcacgccgcg cggcgcggcg cagttcaagg ccaacccgcg gctgcaaacc atcaccagcg
     3841 gctcgcgcgc caagacggtg ctggccatca acaacgccag gaagccgctg aacgacgtgc
     3901 gcgtgcgccg cgccatcagc atggccatcg accgcaaggc cgtcattgcc ggcgccggcg
     3961 acggctttgg cgtgcccatc ggcagccact acgtgccggg cgcggccggc tacatcgaca
     4021 ccacgggcgt caacccctac gacatcgaaa aagccaaagc gctgctgaag gaagccggcg
     4081 tcaccacgcc gctggagctg acgctggtgc tgccgccgcc gccctatgcg cgccagggcg
     4141 gcgaagtcat cgtggcgcaa ctggccaaga tcggtatcgt ggcgaaggtg cagaacgtcg
     4201 aatgggcgca gtggctggcc aacacctacg gcggccccaa gaattacgac ctgaccatca
     4261 tcagccacgt cgagccgttc gacctggcca acttcaccaa ggccgactat tactggggct
     4321 acaacaaccc caagttcaac gcgctgtacg acaagatcaa gaccacgccc aacgtggccg
     4381 agcgcaacaa gctgctgggc gaggcgcaaa agatgctggc cgacgatgcc gtgctggcgt
     4441 ggctctatca gccgcaatgg gtgacggttg ccaagcgtgg cctgaacggg ctgtggaagg
     4501 acatgccgat cttcgtcaac gatctggcgg ctctgtcctg gacttgatct cactataaaa
     4561 acaagagctg ctcgcgcttg tcagacaagc gctggcggct ctttttactt gaagcaccat
     4621 gatgaagacc gctttgcacg acctctccgc gcacgatttg ctggccggct acaaggcgcg
     4681 tgacttctcg cccgtcgagg tcacgcaggc cgtgctggcg cagatcgagc gctgggagcc
     4741 gcagctggcc gccacctact tgctgcgtcc cgaagcagcg ctggcgcagg cccgcgcgtc
     4801 cgaagcgcgc tggttgaagg gcgagccctg cggcacgctg gacggcgtgc cggtcaccat
     4861 caaggacaac atcgccaccc aaggcgaccc ggtgccgctg ggcaccaagg ccgtcgcgct
     4921 cacgccggcc gccgccgacg cgccgcccgc cgcgcgcctg cgcgaatccg gcgccgtcat
     4981 cgtctgcaaa accaccatgc ccgactacgg catgctgtcc tcgggcctgt ccacgtttca
     5041 caagacggcg cgcaacccgt gggatgtgac gcgcacgccc ggcggcagca gcgccggcgc
     5101 cggcgcggca gcggccgcag gctacgggcc gctgcacatc ggcaccgaca tcggcggctc
     5161 gatccgcctg ccggccgcgt ggtgcggcgt cgttggcctg aagcccagct tgggccgcgt
     5221 gccgatcgac ccgccctaca tgggccgcgc cgccggcccc atgacgcgca gcgtggccga
     5281 cgccgc
//
LOCUS       DAOMVO010000162         5275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4333_length_5275_cov_11.240996, whole genome shotgun sequence.
ACCESSION   DAOMVO010000162 DAOMVO010000000
VERSION     DAOMVO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4333_length_5275_cov_11.240996"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1343
                     /gene="rodA"
                     /locus_tag="PKV30_08635"
     CDS             186..1343
                     /gene="rodA"
                     /locus_tag="PKV30_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRL30314.1"
                     /translation="MAVVFEKPSLWQRVWPLLRGFDLLLLAGVLVLAGMGLLAMYSSG
                     FDHGTRFVDHGRNMLIAAGIMFVVAQIPPQRLMSLAVPVYTVGVALLVAVALFGITKK
                     GAQRWVNVGVVIQPSELLKIATPLMLAWWFHRREGQTRPIDFVGAFMLLAIPVGLIMK
                     QPDLGTALLVMAAGLAVIFFAGLPWKVVLPPVIIGLVGIALIVAYETQLCADGLDWRV
                     LHEYQRQRVCTLLDPTRDPLGKGFHILQGMIAIGSGGMYGKGFMQGTQTHLEFIPERT
                     TDFIFAAFSEEFGLAGNLTLIVCFTFLILRGLTIAADAPTLFARLLAGALTTIFFTYA
                     FVNMGMVSGILPVVGVPLPFISYGGTAMVTLGLALGMLMSVAKSKRTLMQS"
     gene            1466..1801
                     /locus_tag="PKV30_08640"
     CDS             1466..1801
                     /locus_tag="PKV30_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRL30315.1"
                     /translation="MAGKFELKKSKNDKFFFSLLAGNGQTILSSEMYEAKANAVNGIE
                     SVKKNAAEAGRFDKLTGKDGSPYFTLKAGNGQVVGQSQMYSSEATRDAGIASVMANAP
                     GAAVDDQTA"
     gene            1935..3623
                     /locus_tag="PKV30_08645"
     CDS             1935..3623
                     /locus_tag="PKV30_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRL30316.1"
                     /translation="MNRPPTFSCRPARWLSALFAALLIACLTGCASLPQDVARPVSHA
                     LAEADGTPLGQLAAARRPAGTAPAVSGYALIDSANLAFSSRLALTQRASKTLDIQYYA
                     IHADQSTARLLREVRAAAARGVRVRILLDDFNSTGQNALVMAMAFLPGVEMRMYNPLP
                     GGRGSTVTRVLGSLHDFKRMQHRMHNKLYIADNSWGITGGRNLGDAYFGTADGSNFVD
                     MDIMSVGPVTRQMSASFDRYWNSPLAYPVQSLISPQELRTLRGTLAPPTQPGAEKAPA
                     PPQPQAAPKPADAASASASAVQAALAPPAAPGDVTDAPALPDALDLATVPLTWAPGRL
                     LVDQPSKLVPELNDEHTDDTVVDGMLHLLQRARRDALIVSPYFVPGPQMMKVFADMRA
                     RGVRVRVLTNSLASNDAPLAHVGYARYREDLIRLGVELYEMRAEQGAKLDRTVFGSSG
                     GGSKASLHSKIFILDGRVISIGSMNLDLRSKLQNTEVALVVRSTELSRDATAKIEDTL
                     DDGAWKMELSPEGRLIWRAPPDAAFGDAHSEPDASLGLRLMLQLLGPFAPDEML"
     gene            3755..5173
                     /locus_tag="PKV30_08650"
     CDS             3755..5173
                     /locus_tag="PKV30_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30317.1"
                     /translation="MGFFGKLLSGLSDRGETAAADTESTAWDFDAWNQIGREKAQRVC
                     SALEAIIDRGEEGDGEDFTDDERSEEFADAVLDEVVALNEAGQWQHARSRFDPAHMPF
                     VSHMGDVGLHAVAILGPERFLVRLDDEVLHIDGGEVTPVAGASMFALSRNRRWLVLAT
                     TDGLIVSPGFGAEPRQTVAWPEGLAVEPDTVRTLDVADDGRTLALASDQFGIWLVKDG
                     AWTALAPRPGVGDDEADESDHAASADAVRNLGTEACPVFFGSYAELKARLGGPLGLDA
                     AHAAVSPDGQWVAYGWQDAPGHYVDRVTATAIEPQGQIAARSDYPYQLRFTDDSGQLL
                     SNSRYRNAGVTVCQDLAALASGDAPQTDDYLRAYGLAELPGGRYGLAEPVAWIGGAGW
                     SHAAPLGGGQPVFTHFLGSALRGYDFDPASGRVAVASASGVLHVLDPFAEAEPGCERG
                     YRPRRELFRWIFWETLDEPIRW"
BASE COUNT          822 a         1766 c         1808 g          879 t
ORIGIN      
        1 acgcacgggc catcgctgcg ctcggccgcg cgcagcgcgc caccgctccg ggctgcgcag
       61 caagcccgct caaaaggccg cggagcaggc cattcgggaa cgccgtggtc ggggtccccc
      121 cgaccaccag cgttgtcccc cccgggggaa ggcgcgtcag cgcctcaggg gggagataat
      181 tctttatggc tgttgtgttt gaaaaaccct cgctctggca gcgcgtgtgg ccgctgctgc
      241 gcgggtttga tctgttgctg ctggcgggcg tgctggtgct ggcgggcatg gggctgctgg
      301 ccatgtattc gtcggggttc gatcacggca cgcgctttgt cgatcacggg cgcaacatgc
      361 tgatcgccgc cggcatcatg ttcgtggtgg cgcagattcc gccgcagcgg ctgatgagcc
      421 tggcggtgcc ggtttacacg gtgggcgtgg cgctgctggt ggcggtggcg ctgtttggca
      481 tcaccaagaa gggcgcgcag cgctgggtca acgtcggcgt ggtgatccag ccgagcgaac
      541 tgctcaagat cgccacgcca ctgatgctgg cctggtggtt tcatcggcgc gaggggcaga
      601 cgcggccgat cgacttcgtg ggcgccttca tgctgctggc gattcccgtc gggctgatca
      661 tgaagcagcc cgacctcggc accgccttgc tggtaatggc ggccgggctg gcggtgatct
      721 ttttcgccgg cctgccctgg aaggtggtgc tgccgcctgt gatcatcggg ctggtgggca
      781 ttgcgctgat cgtcgcctat gagacgcaac tgtgcgccga cgggctcgac tggcgcgtgc
      841 tgcacgaata ccagcgccag cgcgtgtgca cgctgcttga ccccacgcgc gatccgctgg
      901 gcaagggctt tcacatcctg cagggcatga tcgccatcgg ctcgggcggc atgtatggca
      961 agggcttcat gcagggcacg cagacgcacc tcgaattcat ccccgagcgc accaccgact
     1021 tcatctttgc cgcgttttcc gaggaattcg ggctggcggg caacctgacg ttgatcgtct
     1081 gcttcacctt tctcatcctg cgcgggctga cgattgccgc cgacgcgccg acgctgtttg
     1141 cgcgcctgct ggcgggggcg ctgaccacca tcttcttcac ctacgccttc gtcaacatgg
     1201 gcatggtgag cggcatcctg ccggtggtgg gcgtgccgtt gcccttcatc agctacgggg
     1261 gcaccgccat ggtcacgctg gggctggcgc tgggcatgct gatgtcggtc gccaagtcca
     1321 agcgcacgct gatgcagagt tgacgcgcgc gccacacgcg tttgcgcaaa tgcacaccac
     1381 gggttcgcgc ggttgaaaag cgtcttttga cacgccacaa tccgcgccag ccaaccgtgc
     1441 tttggcgacc aatctaggag ttttcatggc aggcaagttc gaactgaaga agtccaagaa
     1501 cgacaagttc tttttctcgc tcctggccgg caatggccag accatcctga gcagcgagat
     1561 gtacgaagcc aaggccaacg cggtcaatgg catcgagtcg gtgaagaaaa acgccgccga
     1621 ggccggccgc ttcgacaagc tcacgggcaa ggacggttca ccctatttca cgctgaaggc
     1681 gggcaacggc caggtcgttg gccagagcca gatgtattcg agcgaagcca cgcgcgacgc
     1741 cggcatcgcc tcggtcatgg ccaacgcgcc cggcgcggcg gtagacgacc agacggcctg
     1801 agagccccgt gttttccgtc cagaaaccgc ctgcgggcgg tttttttgcg cctgtgccgc
     1861 gtctggcgcc gtcgtccgac acgcgccgcg ccggcggcgg ccttagcatc gcggctgcac
     1921 gcccgcccac tgccatgaac cgcccgccca ccttctcctg tcgcccagcg cgctggctgt
     1981 cggccttgtt cgcggcgctg ctgatcgcgt gtctgaccgg ttgcgccagc ttgccgcaag
     2041 acgttgcgcg gccggtatcg cacgccttgg ccgaggccga cggcacgccg ctgggccaac
     2101 tggccgccgc gcgccgcccg gccggcacgg cgccagccgt ctcgggttac gcgctgatcg
     2161 actcggccaa cctggccttc agcagccgcc tggcgctgac gcagcgcgcc agcaaaacgc
     2221 tcgacatcca gtactacgcc atccacgccg accagagcac cgcccgcctg ctgcgtgaag
     2281 tgcgcgcggc cgcggcgcgc ggcgtgcgcg tgcgcatcct gctggatgac ttcaactcca
     2341 ccggccagaa cgcgctggtg atggccatgg cctttttgcc cggcgtggag atgcgcatgt
     2401 acaacccgct gccgggcggc cgcggctcga cggtgacgcg cgtgctcggc tcgctgcacg
     2461 acttcaagcg catgcagcac cgcatgcaca acaagctcta catcgccgac aacagctggg
     2521 gcatcaccgg cgggcgcaac ctgggcgacg cctacttcgg caccgccgac ggcagcaact
     2581 tcgtcgacat ggacatcatg agcgtcgggc cggtcacgcg gcagatgtcg gccagtttcg
     2641 accgctactg gaacagcccg ctggcctacc ccgtgcaatc gctgatttcg ccgcaggagc
     2701 tgcgcacgct gcgcggcacg ctggcgccgc cgacccagcc tggcgctgaa aaagcgcccg
     2761 ccccgccgca gccccaagcc gcgcccaagc ccgcggatgc cgcatcggcc agcgccagcg
     2821 ccgtacaggc cgctctggcg ccgcccgcag cgcccggcga cgtcaccgac gcgcccgcgc
     2881 tgcccgatgc gcttgacctg gccacggtgc cgctcacctg ggcacccggc cggctgctgg
     2941 tggaccagcc gtccaaactg gtgcccgagc tcaacgacga gcacaccgac gacaccgtgg
     3001 tcgacggcat gctgcacttg ctgcagcgcg cgcggcgcga cgcgctgatc gtctcgccct
     3061 acttcgtgcc cgggccgcag atgatgaagg tgtttgccga catgcgcgcc cgtggcgtgc
     3121 gcgtgcgcgt gctgaccaat tcgctcgcgt ccaacgacgc gccgctggcc catgtcggct
     3181 acgcgcgcta ccgcgaagac ttgatccgcc tgggggttga gttgtacgag atgcgcgccg
     3241 agcagggcgc caagctggac cgtaccgtgt ttggctccag cggtggcggc tccaaggcca
     3301 gcctgcattc caagatcttc atcctcgacg ggcgcgtcat cagcattggt tcgatgaacc
     3361 tggatttgcg ctccaagcta caaaacaccg aggtcgcact ggtggtgcgc agcaccgagc
     3421 tgtcgcgcga tgccaccgcc aagatcgagg acacgctgga cgacggcgcc tggaagatgg
     3481 agctgtcgcc cgaagggcga ttgatctggc gcgcaccgcc cgatgccgcg ttcggcgacg
     3541 cgcacagcga gcccgacgcc agcctgggct tgcgcctgat gctgcagctg ctgggcccgt
     3601 tcgcgccgga cgagatgctg taggccaccc accagcgccg gcacggccga tgcgcgcagc
     3661 cgatgctggc gcgacgtgga tcgacaccac gacggcctgc gctcaaagcc gttcacaatg
     3721 cgagctcatc catcggggta gaggagcgcg ttgcatggga tttttcggta agttgctgtc
     3781 gggcttgagc gaccgcggtg aaacagcagc cgctgacacg gaatcaaccg cttgggattt
     3841 cgatgcctgg aaccagattg gccgcgaaaa ggcgcagcgc gtttgttccg ccctggaagc
     3901 catcatcgac cgcggtgaag agggcgacgg cgaagacttc accgacgacg agcgcagcga
     3961 agaatttgcc gacgcggtgc tggatgaagt ggtcgcgctg aacgaggcgg gccaatggca
     4021 gcatgcacgc agccgctttg acccggcgca catgcccttc gtctcgcaca tgggcgatgt
     4081 ggggctgcat gccgtggcca ttctcggccc cgagcgtttt ctggtgcgcc tggacgacga
     4141 ggtgctgcac atcgatggtg gcgaggtcac gccggtggcg ggtgcatcga tgtttgcctt
     4201 gtcgcgcaac cggcgctggc tggtgctggc gacgacggac ggcctgatcg tgtcgcccgg
     4261 cttcggcgcc gaaccgcgcc agaccgtggc gtggccagag ggcttggcgg tcgagccgga
     4321 cacggtgcgc acgctcgacg tggcagacga tggccgcacg ctggcgctgg cgagtgacca
     4381 attcggcatc tggctggtca aggacggcgc ctggacggcg ctggcaccgc gccccggcgt
     4441 cggggacgac gaagcggatg agagcgacca tgcagccagc gcggatgcgg tgcgcaacct
     4501 cggtactgag gcgtgcccgg tgttcttcgg cagctatgcg gagctgaagg cgcgtttggg
     4561 cggcccgctg gggctggacg cggcgcacgc ggccgtttcg cccgacgggc agtgggtggc
     4621 ctacggctgg caggatgcgc cgggccacta tgtggaccga gtgaccgcca ccgccatcga
     4681 gccgcaaggc cagatcgccg cgcgcagcga ctacccgtac cagctgcgtt tcaccgacga
     4741 ttcgggccag ctgctgtcca actcgcgcta ccgcaacgcg ggcgtcacgg tgtgccagga
     4801 cctggcggcg ctggcgagcg gcgatgcgcc gcagaccgac gactacctgc gcgcctacgg
     4861 ccttgccgag ctgccgggtg ggcgctacgg cctggctgag ccggtcgcct ggatcggtgg
     4921 cgccggctgg tcgcacgccg caccgctggg cggcggccag ccggtgttca cgcactttct
     4981 gggttccgcg ctgcgcggct acgactttga cccggcgagc ggccgcgtgg ccgtggccag
     5041 cgcctcgggc gtactgcacg tgctggaccc gttcgccgag gccgagcccg ggtgcgagcg
     5101 cggctaccgc ccgcgccgcg agctgttccg ctggatcttt tgggaaacgc tggacgagcc
     5161 gatccgttgg tagatagaaa atagggccct agggcgtgtc atcaaatgag ccaaacgacg
     5221 gtggcagcca gatgtatgga gcccaggaag gcaatggcag tcttgtcgta gcgtg
//
LOCUS       DAOMVO010000163         5193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4439_length_5193_cov_16.965356, whole genome shotgun sequence.
ACCESSION   DAOMVO010000163 DAOMVO010000000
VERSION     DAOMVO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_5193_cov_16.965356"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2795)
                     /locus_tag="PKV30_08655"
     CDS             complement(<1..2795)
                     /locus_tag="PKV30_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL30318.1"
                     /translation="MFNKVLVANRGEVTLRIVRALCDLGVASVTVYADDDAQAPHVRS
                     ADAAVALGASGPAAYLDGARLIAIALQHGCDALHPGYGFLAERADFARDCAAVGVRFI
                     GPTPDQLALFGDKAAARDLAQRCGVPLVPGVSHAVTLDEARAFFAQHAPNGIVIKAVG
                     GGGGRGMRAIENEIDLSAAYERCRGEALAAFGVADVYVERLMRGARHIEVQVLGDGKQ
                     VMALGERECTLQRRFQKLVEIAPSPSLPDALRAHIVDGALTMARAVRYEGLGTFEFLA
                     DLQSADLPFVFIEANARLQVEHTITEAVTGVDLVQAQIALAAGQSLADLGLDPARAPA
                     PRGYAIQWRINAETLAVDGSATPGHGTLTRFDLPQGPGVRVDTHGFAGAAPSPHYDTL
                     LAKLIVHSPSRDFADVLRRSRRALGEVRIDGVATNLNLLRALAERPEMATQAVHTRWL
                     ESVLPELVDASEKIAARALQTSTDGRSHAENPRIAPMTDAPPGAVLAPMPARLVALSV
                     QVGDSVPAGAELAVLEAMKMQHALNAPHAGVVRAVLVEVGAYVAPGDAILMLEKADHA
                     TDITTAQQAAHDPDHIRADLQRVIDRHARTLDAARPDAMAKRHARNQRTARENIADLC
                     DEGSFIEYGALAIAAQTRRRSVEDLIANTPADGMVTGVGGVNGALFGPERARTAVMAY
                     DATVLAGTQGMRNHAKTDRILNLALQEKLPVVLFAEGGGGRPGDVDANVLAGLHVGTF
                     AAYAALSGQVPVVGIVAGRCFAGNAALVGCSDVIIATKDSNLGMGGPAMIEGGGLGVF
                     APEDVGPSGVQHANGVIDVLVADEQEAVHAARQYLGFFQGHTRDWITPDQRLLRRVVP
                     ENRLRAYDTRAAMQGLVDEGSLLPLRTGFGTGIHTALARIEGRPVGLMANNPLHLGGA
                     IDADAADKGARFMQLCNA"
     gene            complement(2865..4466)
                     /locus_tag="PKV30_08660"
     CDS             complement(2865..4466)
                     /locus_tag="PKV30_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL30319.1"
                     /translation="MTADQPLHEHLRRHAREKPDAPAIVWYGRAISWRELDGWSDALG
                     AHLQSLGVQKGEPVALFMNNCPQYVVAHYAIQKIGAIVCPCGPLNKAHELQYQLDDLK
                     ARTIIAADNLLPIVQQVRGQTALEHVLVVRYGDLLPPEPTIDLPAELQAAPAPLPADA
                     TDMLSIAQRGAQAGQAPAPQNIKMDDIALMTYTSGTTGLPKGAMLSFGNALFKTTVTA
                     QSAGIGPDHVALSVAPLYHIAGMLMGVNVPILVGATQVLLFRFDPVAVLQAIDRYKVT
                     HWYSIAPMNVASMQVPDAARYDLKSLQRNPVTSFGITFTEALAQQWQAFTQGPCLSYE
                     AAYGLSETHTMDTGMPHDAIRWGTHGQPMPGVTIRIVDPDTGVEKPAGEVGEITVRSP
                     GNFKGYWNKPEATSKALNDGWVWTGDQGKLDAEGYLTFIGRTKEMIKVSGYSVFPEEV
                     ESILIKHPAVAQAAVLAEPDVEKGEVVKAFIVKKPGAELAADELIAWSRDNMATYKVP
                     KYVEFIDVLPTTGAGKVLRRLLKAS"
     gene            4559..>5193
                     /locus_tag="PKV30_08665"
     CDS             4559..>5193
                     /locus_tag="PKV30_08665"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRL30320.1"
                     /translation="MQDTYILTLYCPDRPGIVHAVSGFLAERGGNIEEAAQFNDQGTG
                     LFFMRVQFSSPTGDVAPLREQLGALAERFAMRWQLHARAEAMRCVIFVSREGHCLNDL
                     LFRWKSGLLPLDIRAIVSNHRDFYQLAAGYNVPFHHIAVSKANKAQAEERQMEIIEAE
                     GADLVVLARYMQVLSDDMCRRLAGRAINIHHSFLPSFKGAKPYYQAHDRGV"
BASE COUNT          804 a         1773 c         1722 g          894 t
ORIGIN      
        1 gcgttgcaca gttgcatgaa gcgcgcgccc ttgtcggccg cgtcggcatc gatggcgccg
       61 cccaggtgca gcgggttgtt ggccatcagg cccacgggcc ggccttcgat gcgcgccagc
      121 gcggtgtgga ttccagtgcc aaaacccgtg cgcagcggca acagactgcc ttcgtccacc
      181 aagccctgca tggccgcgcg cgtatcgtag gcgcgcaggc ggttttcggg caccacgcgg
      241 cgcagcaggc gctggtcggg cgtgatccag tcgcgggtgt ggccctggaa gaagccgagg
      301 tactggcgcg ccgcatgcac ggcttcttgc tcgtccgcca ccagcacgtc gatcacgcca
      361 ttggcgtgct gcacgccgct cgggcccaca tcctcgggcg cgaacacgcc cagcccgccg
      421 ccttcgatca tggccgggcc gcccatgccc aggttgctgt ccttcgtcgc gatgatcacg
      481 tcgctgcacc ccaccagcgc cgcgttgccg gcaaagcagc gcccggcgac gatgccgacc
      541 accggcacct gacccgacag tgccgcatag gccgcgaaag tgcccacgtg cagaccggcc
      601 agcacgttcg catcgacatc gcccgggcgc ccgcctccgc cttcggcgaa cagcaccacg
      661 ggcagttttt cttgcagcgc caggttcagg atgcggtccg tcttggcgtg gttgcgcatg
      721 ccctgcgtgc cggccagcac ggtggcgtcg tacgccatca cggcggtgcg tgcgcgctcg
      781 gggccgaaga gcgcgccatt gacgccgccc acgcctgtca ccatgccgtc ggccggggtg
      841 ttggcgatca ggtcttccac gctgcggcgg cgcgtttgcg cggcaatcgc cagcgcgccg
      901 tattcgatga agctgccttc gtcgcacaaa tcggcaatgt tctcgcgcgc ggtgcgctgg
      961 ttgcgtgcgt ggcgtttggc catcgcgtca ggccgcgcgg cgtcgagcgt gcgtgcgtgg
     1021 cggtcgatga cgcgctgcag atcggcgcga atgtggtcgg ggtcgtgcgc ggcctgttgg
     1081 gcggtggtga tgtcggtggc gtgatcggct ttttccagca tcaggatcgc gtcgccgggt
     1141 gccacgtacg cgccgacttc gaccaacacg gcgcgcacca cgccggcgtg cggcgcattc
     1201 aaggcgtgct gcatcttcat ggcttccagc acggcgagtt cggcgccggc aggcacgctg
     1261 tcaccaacct gcaccgacag cgccaccaac cgcgccggca tgggcgccag cacagcgccg
     1321 ggcggtgcgt cggtcatcgg tgcgatgcgc ggattttcag catgacttcg gccgtcagtg
     1381 cttgtctgta aagcgcgagc agctatcttt tcagaagcat caacgagttc cggcaacacc
     1441 gattccagcc agcgcgtgtg caccgcttgc gtcgccattt cgggccgttc ggccagtgcg
     1501 cgcagcagat tcaggttggt ggccacgccg tcgatgcgca cctcacccag cgcgcggcgc
     1561 gagcggcgca gcacgtcggc gaagtcgcgg ctgggcgagt gcacgatcag cttggccagc
     1621 agcgtgtcgt agtgcggcga tggcgcggcg ccggcaaagc catgcgtatc gacgcgcacg
     1681 ccggggcctt gcggcaaatc gaagcgcgtg agcgtgccgt ggccgggtgt ggcgctgcca
     1741 tcgaccgcga gcgtttcggc attgatgcgc cactggatcg cgtagccgcg cggcgcgggc
     1801 gcgcgggccg gatccagccc cagatcggcc aggctttgcc ccgccgcaag tgcaatctgc
     1861 gcctgcacca gatcgacgcc ggtgacggct tcggtgatgg tgtgctccac ctgcaggcgc
     1921 gcgttggcct cgatgaagac gaagggcagg tcggcggatt gaagatcagc caggaactca
     1981 aacgtgccca gcccttcgta gcgcacggcg cgcgccatgg tgagcgcgcc gtccacgatg
     2041 tgcgcgcgca gcgcatcggg caggctgggg ctgggcgcaa tctccaccag cttctgaaag
     2101 cgccgctgca aggtgcattc acgctcgccc agcgccatca cctgcttgcc gtcgcccagc
     2161 acctgcacct cgatgtggcg tgcgccgcgc atcaggcgct ccacatacac atcggcaacg
     2221 ccgaaggcgg ccagcgcctc gccccggcag cgttcgtagg cggcgctcag atcaatttcg
     2281 ttttcaatcg cccgcatgcc gcgcccgcca ccgccgccca cggccttgat gacgatgccg
     2341 ttcggtgcgt gctgcgcgaa gaaggcacgt gcttcgtcca gcgtgaccgc gtgcgacacg
     2401 ccgggcacca gcggcacgcc gcagcgctgc gccaagtcgc gtgcggcggc cttgtcgccg
     2461 aacagcgcca gttgatcggg tgtcgggccg atgaagcgca cgcccaccgc cgcgcagtct
     2521 cgtgcgaagt cggcgcgctc ggcgagaaaa ccgtagccgg ggtgcagcgc gtcgcaaccg
     2581 tgctgaagcg caatggcgat caaccgcgcg ccatccaggt acgccgccgg cccgctggcg
     2641 cccagcgcca ccgccgcgtc ggccgagcgc acatgcggcg cctgcgcgtc gtcgtcggcg
     2701 tacaccgtga cgctcgccac acccagatcg cacaaggcgc gcacgatgcg cagcgtcacc
     2761 tcgccacggt tggccaccag aaccttgttg aacatgattg cttccaaatc aggagctgcc
     2821 tgcgcttgat ttacctgcgc taacggccaa aaacacttga accctcaact cgccttcaac
     2881 aagcgccgca gcaccttgcc cgcgcccgtg gtcggcagca catcgatgaa ctcgacgtac
     2941 ttgggaactt tgtaggtcgc catgttgtcg cggctccagg cgatcagttc atccgccgcc
     3001 aactcggcgc ccggcttctt gacgatgaag gccttgacta cctcgccctt ttccacatca
     3061 ggctccgcca gcaccgccgc ctgcgccacg gcggggtgtt tgatgaggat gctctccacc
     3121 tcttccggga acacgctgta gcccgagacc ttgatcatct ccttggtgcg gccgatgaag
     3181 gtgaggtagc cctcggcgtc cagcttgccc tggtcgcccg tccacaccca gccgtcgttc
     3241 agcgccttgg acgtggcctc gggcttgttc cagtagccct tgaagttgcc cggcgagcgc
     3301 acggtgattt cgcccacttc gcccgccggt ttttcaacgc ccgtgtcggg atcgacgatg
     3361 cggatcgtca cgcccggcat cggctgcccg tgcgtgcccc agcggatggc atcgtggggc
     3421 atgccggtgt ccatggtgtg cgtttcgctc aggccgtagg cggcttcgta agacaggcac
     3481 ggcccctgcg tgaacgcctg ccattgctgc gccagcgcct cggtaaaggt gatgccaaaa
     3541 ctggtaactg gattgcgctg caagctcttc aaatcgtagc gagcggcatc cggcacctgc
     3601 atgctggcga cgttcatcgg ggcgatggaa taccagtgcg tgaccttgta gcggtcaatg
     3661 gcttgcagaa ccgccaccgg atcaaagcgg aacagcagca cctgcgtcgc gcccacgagg
     3721 atgggcacgt tcacgcccat caacatgccg gcgatgtgat acagcggcgc gaccgacagc
     3781 gccacatggt ccggcccgat gcccgcggac tgcgccgtca ccgtggtctt gaagagtgcg
     3841 ttgccaaagc tcagcatcgc gcccttcggc aagccggtgg tgccgctggt gtaggtcatc
     3901 agggcgatgt cgtccatctt gatgttttgg ggcgctggcg cttgccccgc ctgcgcaccg
     3961 cgctgcgcaa ttgagagcat gtctgtggcg tcggcgggca gcggtgcggg cgcggcctgt
     4021 agttccgctg gcaagtcgat agtcggttct ggcggcagca ggtcgccata gcgcaccaca
     4081 agcacgtgct ccagcgccgt ctgcccgcgc acttgctgga cgatcggcag caggttgtcg
     4141 gcggcgatga tggtgcgcgc cttcaggtca tcaagctggt attgcagctc gtgcgccttg
     4201 ttcagcggcc cgcacgggca cacgatggcg ccgatcttct ggatcgcgta gtgcgccacc
     4261 acgtactgcg ggcagttgtt catgaacagc gccaccggct cgcccttttg cacgcccagc
     4321 gactgcaggt gcgcgcccag ggcgtcgctc cagccgtcca gctcgcgcca gctgatggcg
     4381 cggccatacc agacgatggc tggcgcatcg ggcttttcgc gcgcgtggcg gcgcaggtgt
     4441 tcgtgcaggg gctggtcggc ggtcatgggg cgtcctcggt gatcggttgc gggtcaatat
     4501 agccacgcgg cggcctgggc ggcgcgggct gccatcgcct gcgcgacaat cgcgcccgat
     4561 gcaagacacc tacatcctga ccctttactg ccccgaccgc ccgggcatcg tgcacgccgt
     4621 gtcgggcttt ctggccgagc gcggcggcaa catcgaagag gcggcgcagt tcaacgacca
     4681 aggcaccggc ctgttcttca tgcgcgtgca attcagcagc ccgaccggcg atgtggcgcc
     4741 gctgcgcgag cagttgggcg cgctggccga gcgctttgcc atgcgctggc aactgcacgc
     4801 gcgcgcagag gccatgcgct gcgtgatctt cgtcagccgc gaagggcatt gcctgaacga
     4861 tttgttgttc cgctggaaga gcggcctgct gcccttggat atccgtgcca tcgtcagcaa
     4921 ccaccgtgac ttctaccaat tggcggccgg ctacaacgtg ccgtttcacc acatcgccgt
     4981 cagcaaggcc aacaaggcgc aggccgagga gcggcagatg gaaatcatcg aggccgaggg
     5041 cgccgatctg gtggtgctgg cgcgctacat gcaggtgctg tcggacgaca tgtgccgccg
     5101 gctggccggg cgcgcgatca acatccacca cagctttttg cccagcttca agggcgccaa
     5161 gccgtattac caggcgcacg accgcggcgt gaa
//
LOCUS       DAOMVO010000164         5181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4452_length_5181_cov_15.204643, whole genome shotgun sequence.
ACCESSION   DAOMVO010000164 DAOMVO010000000
VERSION     DAOMVO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4452_length_5181_cov_15.204643"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKV30_08670"
     CDS             complement(<1..494)
                     /locus_tag="PKV30_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRL30321.1"
                     /translation="MSEQAPQRLSARTAIVGTDTPIARVLPQAERRTIGAWCFLDHAG
                     PVSFAPGGGLRVGAHPHTALQTFTWMLEGEVMHNDSLGHRQVVRPGQVNLMTAGHGIA
                     HTEESLPDQTRLHAAQLWIALPAHAADIAPRFDHYPDLPRWQQDSVDVVLLAGEWQGR
                     RAPAL"
     gene            complement(491..1105)
                     /locus_tag="PKV30_08675"
     CDS             complement(491..1105)
                     /locus_tag="PKV30_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRL30322.1"
                     /translation="MAAATPVRADDWLLTPENAVITLIDYQPDQYAGVGSIGVDELLV
                     NVITLGQIATAYGLPVVLSTVAACRAPTKSCTRRSTTRPKSTAPRSTRGKTRTFAPPS
                     TGRKKLIVAGLWTEICVALPVLDALHDGYDLYVVEDAIGGVSPAAHHAAMRRMEQAGA
                     RPITVIGLAGELQRDWGREGTDRLRAVLGSYFGGHRGLKSGGRP"
     gene            complement(1129..1434)
                     /locus_tag="PKV30_08680"
     CDS             complement(1129..1434)
                     /locus_tag="PKV30_08680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL30323.1"
                     /translation="MTASELPEVTLNEAAHRFELHVDGHTAVEVFERFPGGIAYLHTI
                     VPKALEGRGVGSKLVRHIFEYAVQNQLKVRPDCPFVKAYIDKHPEYHPISLAHGATL"
     gene            complement(1452..3257)
                     /locus_tag="PKV30_08685"
     CDS             complement(1452..3257)
                     /locus_tag="PKV30_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRL30324.1"
                     /translation="MAAEGYARQLIGVVTLLGAAVVAVPLFKRIGLGSVLGYLAAGLA
                     IGPFGLALVSDPQSILHLAELGVVMFLFIIGLEMKPSHLWSLRKQIFGLGSLQVVVCG
                     ILLTLVGVVGYNAPWPVAFVAAMGFVLTSTAIVMQVLAERGDVNAPRGQRMVSILLLE
                     DMLIVPLLALVAFLAPADAAHAAADPLWQRLGVAALALGALVGAGLWLLNPLFRILAK
                     AGAREVMTAAALLVVLGAALLMELGGLSMAMGAFVAGVLLSESSFRHQLEADIEPFRG
                     LLLGLFFLAVGMSLNLSVVRDNWINIATGVVALMAVKALCIYAVARFAGSSKADATDR
                     AVLMAQGGEFAFVLYSAALAQNVISENINAVMTAIVVLSMVLTPLVVVLHKRFARAEA
                     PSLDGVDTPHDLHGNVLIIGFGRVGQIASQAPLARGATLSVIDLDTEVIQTAARYLNF
                     KVYYGDGARPEVLHAAGAHHADVIIVCVDKPEAATRIAENVRSQCPNARLVVRAFDRE
                     HALALVKMDVDTVVRETFDAALRMGHEAVRAMGASDIEAQEITEDVRRRDAERFALEI
                     AGKPFAGRALVLGNIDHIEPPPPKDKPAEPPPNPA"
     gene            complement(3369..3602)
                     /locus_tag="PKV30_08690"
     CDS             complement(3369..3602)
                     /locus_tag="PKV30_08690"
                     /inference="COORDINATES: protein motif:HMM:NF022397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HRL30325.1"
                     /translation="MNDQNVLKHINDLVAEEQRLRDAPADADPSGKRLRDVEVQLDQC
                     WDLLRQRRARREFGRNPDDADVRDAGTVEGYTG"
     gene            complement(3631..4035)
                     /locus_tag="PKV30_08695"
     CDS             complement(3631..4035)
                     /locus_tag="PKV30_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRL30326.1"
                     /translation="MRPPSRPSPAGDWVGFRKQALPLLDAHLGHLHGHHRLEDQHYFP
                     AMRRAEPRLDKGFDLLAADHERIDASVRELQRLRTLLAHASPAEAAAWQCAHDMARAL
                     PVCGTLLAQHLGDEEDLVIPLLALREAAPRAA"
     gene            complement(4163..5080)
                     /locus_tag="PKV30_08700"
     CDS             complement(4163..5080)
                     /locus_tag="PKV30_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRL30327.1"
                     /translation="MTTATTEVRTATATTTAKKVLDTRSAPGRHWVGDGFPVHGLFGY
                     NGAGVAERSPFLMLDYAAPTRFEPNSGYRRGVGQHPHRGFETVTIVYDGEVEHRDSTG
                     AGGVIGKGDVQWMTAGGGIIHEEFHSEAFSKTGGPFEMVQLWVNLPARDKMTPAHYQG
                     ITDASIPSVALPDGAARVRVIAGEFDGNKGPAETYTPMNVWDLRLSAGKPVDLAQPEG
                     WSTLVVVLDGTVLLNGDTVLRAADMATLSSAGSGVTIEANGDAKVLLLAGEPIDEAVV
                     GYGPFVMNSQQEIAEAISDFNSGKFGRIG"
BASE COUNT          808 a         1752 c         1798 g          823 t
ORIGIN      
        1 agcgccggcg cgcgccggcc ctgccattca ccggccagca gcaccacgtc gacactgtcc
       61 tgctgccagc gcggcagatc agggtagtgg tcgaagcgtg gcgcgatgtc ggccgcgtgc
      121 gcgggcagcg cgatccacag ttgcgcggcg tgcaggcggg tctggtcggg taacgattct
      181 tcggtgtggg cgatgccgtg gccggccgtc atcaggttga cctggccggg gcggacgacc
      241 tggcggtggc ccaggctgtc gttgtgcatc acctcgcctt ccagcatcca ggtgaaggtc
      301 tgcagcgcgg tgtgcgggtg cgcgcccacg cgcaggccgc cgcccggtgc aaagctgacc
      361 gggcccgcgt ggtcgagaaa gcaccaggcg ccgatggtgc ggcgctcggc ttgcggcagc
      421 acgcgggcga tgggcgtgtc ggtgccgacg atggcggtgc gcgcggacag gcgctggggc
      481 gcttgttcgc tcatggccgc cccccgctct tcaagccgcg gtggccgcca aagtagctgc
      541 ccagcaccgc gcgcaggcgg tcggtgcctt cgcggcccca gtcgcgctgc agttcgccgg
      601 cgaggccaat cacggtgatg ggcctggcgc cggcctgttc catgcggcgc atggcggcat
      661 ggtgcgccgc ggggctgacg ccgccgatgg cgtcttccac cacgtacaag tcatacccgt
      721 cgtgcagggc gtcgagcacg ggcaaggcga cgcagatttc cgtccacagc ccggccacga
      781 tgagcttctt gcggcccgtc gatggcggcg cgaaagtccg ggtcttccca cgagttgatc
      841 gtggtgcggt cgatttcggg cgcgttgttg agcgccgcgt gcagctcttt gttggtgccc
      901 tgcatgccgc gacagtggac agcaccacgg gcaggccgta ggcggtggcg atctggccga
      961 gcgtgatgac gttgaccagc agctcatcga cgccgatgga gccgacgccc gcgtactggt
     1021 cgggctggta gtcgatcaag gtgatgacgg cgttttccgg ggtgagcagc cagtcgtcgg
     1081 cgcgaacggg agtggcagcg gccatggggg tctccttggg cggtcggatc aaagtgtggc
     1141 gccgtgcgcc agcgagatcg ggtggtattc agggtgcttg tcgatgtacg ccttgacgaa
     1201 ggggcagtcg ggtcgtactt tgagctggtt ctgcaccgcg tactcgaaga tgtggcgaac
     1261 cagcttgctg ccgacgccgc gcccttccag cgccttgggc acgatggtgt gcaggtaggc
     1321 gatgccgccc ggaaagcgct cgaatacctc gactgcggtg tggccgtcca cgtgcagctc
     1381 gaaacggtgc gcggcttcgt tgagggtgac ttcgggcagc tcgctggcgg tcatgcggct
     1441 ctcctggtgg ttcaagcggg gttgggtggc ggctcggccg gcttgtcctt gggcggcggc
     1501 ggctcgatgt ggtcgatgtt gcccagcacc agcgcgcggc cggcaaatgg cttgccggcg
     1561 atttccagcg caaagcgctc ggcgtcgcga cggcgcacgt cctcggtgat ttcctgcgcc
     1621 tcgatgtcgc tggcgcccat ggcgcgcacg gcttcgtggc ccatgcgcag cgcggcgtcg
     1681 aaggtttcgc gcaccacggt gtcgacgtcc atcttcacca gcgccagcgc atgctcgcgg
     1741 tcgaaggcgc gcaccaccag ccgcgcgttg gggcactggc tgcgcacgtt ttcggcaatg
     1801 cgggtggcgg cttcgggctt gtcgacacag acgatgatga cgtcggcatg gtgcgcgccg
     1861 gcggcgtgca gcacctcggg ccgcgcgccg tcgccgtagt acaccttgaa gttgaggtag
     1921 cgcgctgccg tctggatgac ctcggtgtcg aggtcgatca cgctcaaggt ggcgccgcgc
     1981 gccagcggcg cctggctggc gatttggcca acacgtccaa agccgatgat gagaacgtta
     2041 ccgtgcaggt cgtgcggcgt atccacgccg tcgagcgagg gcgcctcggc gcgcgcaaag
     2101 cgcttatgca gcaccacgac cagcggcgtc agcaccatcg acagaaccac gatggccgtc
     2161 atcaccgcgt tgatgttctc ggaaatgacg ttttgcgcca gtgccgccga atacagcacg
     2221 aaggcgaatt caccgccctg cgccatcagc acggcgcggt cggtggcatc ggccttgctg
     2281 ctgccggcaa agcgcgccac agcgtagatg cacagcgcct tgaccgccat cagcgccacc
     2341 acgccggtgg cgatgttgat ccagttgtcg cgcaccaccg acaggttgag cgacatgccg
     2401 acggccagga aaaacaagcc cagcagcagg ccgcggaagg gctcgatgtc ggcctccagc
     2461 tggtggcgaa agctcgattc cgacagcagc acgccggcca cgaaggcgcc catggccatc
     2521 gacaggccgc ccagttccat cagcagcgcg gcgcccagca ccaccagcag cgcggcggcg
     2581 gtcatgacct cgcgcgcgcc ggccttggcg aggatgcgaa acaagggatt cagcagccac
     2641 agccccgcgc cgaccagcgc gcccagcgcc agcgcggcga cgcccaggcg ctgccacagt
     2701 ggatcggcgg cggcgtgcgc ggcatcggcc ggcgccagaa aggccaccag cgccagcagc
     2761 ggcacgatga gcatgtcttc caaaagcagg atcgacacca tgcgctgccc gcgcggcgcg
     2821 ttgacgtcgc cacgctcggc cagcacctgc atgacgatgg ccgtcgacgt gagcacaaaa
     2881 cccatcgccg ccacaaacgc caccggccac ggcgcgttgt agcccaccac gcccaccagc
     2941 gtgagcaaga tgccgcacac caccacctgc aggctgccca ggccgaagat ctgcttgcgc
     3001 aggctccaca ggtgcgaggg cttcatctcc agcccgatga tgaacaggaa catcaccacc
     3061 cccaactccg ccaggtgcag gatggactgc ggatcgctca ccagcgccag cccgaacggc
     3121 ccgatggcca gcccggccgc cagatagccc agcaccgagc caagcccgat gcgcttgaac
     3181 aagggcacgg ccaccacggc cgcgcccagc aacgtcacca cgccgatcaa ttgccgggca
     3241 tacccttcag cagccatgaa tgcgctttct gttcttcttg ttcattgatg gcgggccggt
     3301 gctgccggcc caccgaaaac cccgttcgcg cagagcctgt cgaagcgccg cgcgccgccc
     3361 gcctgccgtc agccggtgta gccttcgacc gtgccggcgt cgcgcacatc cgcgtcgtcc
     3421 gggttgcggc cgaattcacg gcgcgcgcgg cgctggcgca gcaggtccca gcactggtcg
     3481 agctggactt cgacatcgcg caggcgcttg ccgctcgggt cggcatcggc gggggcatcg
     3541 cgcaggcgct gctcctcggc cacgaggtcg ttgatgtgct tcaggacgtt ctggtcgttc
     3601 atgctcgggc ctcgtatcga agtgagtgga tcaggctgcg cgcggggcgg cctcgcgcag
     3661 cgccagcagc gggatgacca agtcttcctc gtcacccaaa tgctgcgcca gcagcgtacc
     3721 gcaaaccggc aacgcccgcg ccatgtcgtg cgcgcattgc cacgccgccg cctcggccgg
     3781 gctggcgtgc gccagcagcg tgcgcaggcg ctgcaactcg cgcaccgacg cgtcaatgcg
     3841 ctcgtggtcg gcggccagca ggtcaaagcc tttgtccaga cgcggctcgg cacggcgcat
     3901 ggcgggaaag tagtgctggt cttccagccg atggtggccg tgcaggtggc ccaggtgggc
     3961 gtcgagcagg ggcagcgcct gcttgcgaaa gccgacccag tcccccgctg gggaaggtcg
     4021 ggatgggggc cgcaccggct gccagatcaa ccggggtagc ccccacccca gccctccccc
     4081 agcgggggag ggagaacagc cgtccgcgaa aacgtcgcgg gccccgaaag gcccgcgact
     4141 tgccagcgca aacagcgccc catcagccaa tgcggccgaa cttgccgctg ttgaagtcgc
     4201 tgatggcttc agcgatttcc tgctggctgt tcatcacgaa cggcccgtag cccaccaccg
     4261 cctcgtcgat cggctcgccg gccagcagca gcaccttggc gtccccattg gcctcgatgg
     4321 tcacgccgct gccggcagac gacagcgtgg ccatgtcggc ggcgcgcagt acggtgtcgc
     4381 cgttcagcag caccgtgccg tcgagcacga cgacgagggt gctccaacct tccggctgcg
     4441 ccaggtccac cggtttgccg gcactcaggc gcagatccca tacgttcatg ggcgtgtagg
     4501 tttcggccgg ccctttgttg ccgtcgaatt cacccgcgat cacgcgcacg cgggctgcac
     4561 catccggcaa agccaccgac ggaatgctgg cgtcggtgat gccctggtag tgcgccgggg
     4621 tcatcttgtc ccgggcgggc aggttgaccc acaactgcac catctcgaac gggccgccgg
     4681 tcttgctgaa ggcctcggag tggaactcct catggatgat gccgccgccg gcggtcatcc
     4741 actgcacgtc gcccttgccg atgacgccgc cggcgcccgt gctgtcgcgg tgctccactt
     4801 cgccgtcgta gacgatggtc acggtctcga agccgcggtg cgggtgctgg cccacgccgc
     4861 gccggtagcc gctgttgggc tcaaagcgcg tcggcgcggc gtagtcgagc atcaggaacg
     4921 ggctgcgctc ggccacgccg gcgccgttgt agccgaacag gccgtgcacc ggaaaaccgt
     4981 cgccgaccca atggcggccg ggggcgctgc gggtgtcgag cactttcttg gcagtggtgg
     5041 tggcggtggc ggtgcggact tcggtggtgg cggtggtcat gtggataacc tctcttcgat
     5101 gtcgcgcgga ttcagagccg cgctgcgtag ggcgccaacc ggtctgtcag cgcatgattg
     5161 aatggtatgg gcaatcgaat c
//
LOCUS       DAOMVO010000165         5179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4454_length_5179_cov_15.908470, whole genome shotgun sequence.
ACCESSION   DAOMVO010000165 DAOMVO010000000
VERSION     DAOMVO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_5179_cov_15.908470"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKV30_08705"
     CDS             <1..369
                     /locus_tag="PKV30_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRL30328.1"
                     /translation="TPAPAPAPAPVPAPPAPAPAPAPPAPPVVVQPSSNAAYLNNPPP
                     PYPPMSKRLGETGRVIVRVLIGPNGRAQEARIQRSSGFERLDEIALETARDRWRYVPG
                     TRNGVPEAMWFNVPINFVLE"
     gene            376..1092
                     /locus_tag="PKV30_08710"
     CDS             376..1092
                     /locus_tag="PKV30_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRL30329.1"
                     /translation="MDNNGFGLMHVWAQGDWVTRCVFAALVLMSVASWIVIVIKALGV
                     VRAKGQAARVEGFWHSSDMAEGLSKLGTDDTNPFRQIAIEGREAAAHHRATKAQLHDT
                     LDASDWISRALRNSIDDTTGQLQAGLAILASVGSTAPFVGLFGTVWGIYHALMKIGSA
                     GQASIDQVAGPIGEALIMTAMGLAVAIPAVLGYNALVRGNKHILAKLGRFAHDLHAYY
                     VTGARVTAASDGKVVAMKKG"
     gene            1099..1512
                     /locus_tag="PKV30_08715"
     CDS             1099..1512
                     /locus_tag="PKV30_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRL30330.1"
                     /translation="MAGFTADDTDEVMSEINMVPFIDVMLVLLIIFIITVPVMKHAVN
                     VQLPKVSNEKAVDKPETVRLTVQADGSYALGEEKIADEQLAPRLQAEAAKQPQPDLHI
                     RGDKDVRYERVAQAMAAAQQAGLSKIGFITEPPKN"
     gene            1549..1725
                     /locus_tag="PKV30_08720"
     CDS             1549..1725
                     /locus_tag="PKV30_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HRL30331.1"
                     /translation="MPPAPEALLPAARPAQAHAPAPLPSDQLLQGRKAVEISHNGSVY
                     RLQATKLGKLILTK"
     gene            1956..2852
                     /locus_tag="PKV30_08725"
                     /pseudo
     CDS             1956..2852
                     /locus_tag="PKV30_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913506.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2929..4218)
                     /gene="boxA"
                     /locus_tag="PKV30_08730"
     CDS             complement(2929..4218)
                     /gene="boxA"
                     /locus_tag="PKV30_08730"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HRL30332.1"
                     /translation="MNTSAILKQHLIDPEICIRCNTCESICPVQAITHDSRNYVVDAS
                     KCNFCMDCISPCPTGSIDNWRLVPKSQPYTLDEQFSWDDLPAEKTPEQLAELGVAEGA
                     TNSAEDLVAQAQMDAAAQDPTGEAPFNSSAFGATTPPWSAAHAYANLYGPKAADKFVT
                     ATVVGNVRVTEVGTEYDTHHLMLDFGAMPFPVLEGQSIGIIPPGVDDRGKPHHPRQYS
                     IASPRNGERPGYNNISLTIKRVLEDHQGQPVRGVASNYMCDLKVGDTVQVTGPFGTSF
                     LMPNHPRSNIIMICTGTGSAPMRAMTEWRRRLRKTGKFEGGKLMLFFGARTQQELPYF
                     GPLQGLPKDFIDINFAFSRTPGAAKKYVQHALRERAADVAPLLQDANTFIYVCGLKSM
                     EEGVVLALRDVVQGAGLNWGEIGGRLKKEGRLHLETY"
     gene            complement(4368..>5179)
                     /locus_tag="PKV30_08735"
     CDS             complement(4368..>5179)
                     /locus_tag="PKV30_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HRL30333.1"
                     /translation="MFTYFTDRDGKFQLCALAESSFDPLARTTKFMLTEEAHHMFVGE
                     SGVGRVIQRTVDMMNQLKTDDPAKLRAAGVIDLPTIQRYLNFHFSVTIDLFGADESSN
                     AATFYSTGLKGRYEEGKRQDDHVLKGQSYKVLNVQDGKLTEKEVPMLNALNEVLRDDY
                     IKDSVGGVERWNRIIDKAGIPFKLKVPHKAFHRNIGALAGVKVSPEGQVVSEAEWKAK
                     VDQWLPTPEDRAYVASLMGRVVEPGKFAGWIAPPVMGINRQPVDFEYVRFN"
BASE COUNT          826 a         1686 c         1723 g          944 t
ORIGIN      
        1 acgcccgcgc ccgcgccggc cccagcgccc gtgccggcac cgcctgcacc agcgcccgcg
       61 ccggcaccgc cagcgccgcc ggtggtggtg cagccgtcgt cgaatgccgc ctacctgaac
      121 aacccgccgc cgccctaccc gcccatgagc aagcgcctgg gcgagaccgg ccgggtcatc
      181 gtgcgggtgc tgattggccc caacggccgc gcgcaagagg cgcgcatcca gcgctccagc
      241 ggcttcgagc ggctggacga gatcgcgctg gaaaccgcgc gcgaccgctg gcgctacgtg
      301 cccggcacac gcaatggcgt gcccgaggcg atgtggttca acgtaccgat caattttgtg
      361 ttggagtgaa gtgaaatgga caacaacgga tttggcttga tgcacgtgtg ggcccagggc
      421 gactgggtca cgcgctgcgt gttcgcggcg ctggtcttga tgtcggtggc ctcgtggatc
      481 gtgatcgtga tcaaggcgct gggtgtggtg cgcgccaagg gccaggcggc gcgcgtcgaa
      541 gggttctggc actcgtcgga catggccgag ggcctgtcca agctgggcac cgacgacacc
      601 aacccgttcc gccagatcgc cattgaaggg cgcgaagccg ccgcgcacca ccgcgccacc
      661 aaggcacagt tgcacgacac gctggacgcc agcgactgga tctcgcgcgc cctgcgcaac
      721 agcatcgacg acaccacggg ccagctgcaa gcgggcctgg cgatcctcgc ctcggtcggc
      781 tccaccgccc ccttcgtggg cctgttcggc accgtctggg gcatctacca cgcgctgatg
      841 aagatcggct cggccggcca ggccagcatc gaccaggtgg ctggcccgat cggcgaggcg
      901 ctgatcatga ccgccatggg cctggcggtg gccattcccg ccgtgctggg ttacaacgcg
      961 ctggtgcgcg gcaacaagca cattctggcc aagctggggc gcttcgcgca cgacctgcac
     1021 gcctactacg tgaccggcgc gcgcgtgacg gcggccagcg acggcaaggt tgtcgccatg
     1081 aaaaaggggt aagcggccat ggccggattt accgccgacg acaccgacga ggtgatgagc
     1141 gagatcaaca tggtcccgtt catcgacgtg atgctggtgt tgctgatcat cttcatcatc
     1201 accgtgccgg tgatgaagca tgccgtcaac gtgcaactgc ccaaggtcag caacgaaaaa
     1261 gccgtcgaca agcccgagac cgtgcgcctg accgtgcagg ccgatggcag ctacgccctg
     1321 ggcgaagaga agatcgccga cgagcagttg gccccgcgcc tgcaggccga ggccgccaag
     1381 cagccgcagc ccgacctgca catccgcggc gacaaggacg tgcgctatga gcgcgtggcg
     1441 caggccatgg cggcggcgca gcaggccggc ctttcaaaaa tcggcttcat caccgagccg
     1501 cccaaaaact gaggaaggag cccgcgcatg accccaccgc gcatcgacat gccgccagcg
     1561 cccgaggcgc tgctgcccgc cgcccgcccg gcgcaggccc acgcgccggc gccgctgccc
     1621 agcgaccagt tgctgcaagg ccgcaaggcc gtcgagatca gccacaacgg ctcggtttac
     1681 cgcctgcagg ccaccaagct gggcaagctg atcctcacca agtgacgggc aggcccgccg
     1741 ccgttcactg tttttcaagt ttcatcgtgg gaagcgcgtc acgcgctttt taaatgcaag
     1801 ccaggccgta agacccagcc agcatctttt caccccccac gcagcgcgcc gccaatacca
     1861 agccggcgcg agcggatcgc gtaccatgca ggcgagcatc gggtcgcacg atcccgcccc
     1921 tgcaacgaac ccagccaccc cccatacaca aggccatgac gcgctggtac tttggctgga
     1981 acatcgttgc cgcatcggcg ctgctgacct tgctgtcgtc gggcatgcgg ctcagcatcg
     2041 gcccgttctt cctgcccatc gcgcaagacc tgggcttcag ccgcagcctg ctggcgtccg
     2101 aagcttttgg ctggcgcgcc acgctggtcg gctttgccgc cacgctgacg ccggtggcca
     2161 tcccagccgc actgttcatc atgcgcgacc aggcacccga aggcaccgac ctgcgtccga
     2221 ccaacgccac ggctgctgca gcccccgctg cgcccgcgcc cgtcttgagt ctgcgcgacg
     2281 ccttgcgcac tgcgccgttc tagatgatct ttggcggcct gttcgcctgc ggctacagca
     2341 tgaacctgtt gggcacgcac ggcgtgccga tgctgatgga ccacggcttt gacgcccaca
     2401 gcagttcgat gggcatcggc ctgatcggct ttgtcgccat cttcggcacg ctggtgctgg
     2461 gccgctggtc agaccggctg ccgcgccgca acatcctggc ggccatctac ggcgtgcgcg
     2521 ggctgggctt tttcgcgctg atgatcgtgg gcacgcattg ggagctgtac gtcaccgcca
     2581 gcatcggcgg gctggtgtgg gccggcagca tcgcgctgtc gtcggccatc ctggcggatt
     2641 tgtacggcgt gcggctggtg ggcgtgctgt acgggctggc gtacgtgggc caccagttgg
     2701 gcggcatgct gagcgcctgg ctcggcggct ggggctacga gcgctttggc acgcactggg
     2761 tggcgtttgg cgcctccggt gcgctgctgc tggccgccgc cgtggtggca ttgcagttgc
     2821 cgacgcggcg gccggtggcg gtgccggcgt agcgcgcacc tactgatcaa aacaggcgct
     2881 ggcgcttgtc agacaagcgc gagcagctat caaaaaagaa gcaaccgctc agtaggtttc
     2941 caggtgcagc cggccttctt tcttcaggcg gccgccgatc tccccccagt tgagccccgc
     3001 gccctgcacc acgtcgcgca gggccagcac cacgccttct tccatgcttt tgaggccgca
     3061 gacgtagatg aaggtgttgg cgtcctgcag cagcggcgcc acatcggcgg cgcgttcacg
     3121 cagcgcgtgc tgcacatact tcttggccgc gccgggcgtg cggctgaagg cgaaattgat
     3181 gtcgatgaag tccttgggca agccttgcag cgggccgaag tacggcagtt cttgctgcgt
     3241 gcgggcgcca aagaacagca tcagcttgcc gccttcgaac ttgcccgttt tgcgcagccg
     3301 ccggcgccat tcggtcatgg cgcgcatggg ggccgagcct gtgccggtgc agatcatgat
     3361 gatgttcgaa cgcgggtggt tgggcatcag gaagctggtg ccgaacgggc cggtcacttg
     3421 cacggtgtcg cccaccttca ggtcgcacat gtagttgctg gccacgccgc gcacgggctg
     3481 gccctggtgg tcttcgagca cgcgcttgat ggtcaggctg atgttgttgt agccgggccg
     3541 ctcgccgttg cgcgggctgg cgatgctgta ctggcgcggg tggtgcggct tgcctcgatc
     3601 atccacgccg ggcgggatga tgccgatgct ctggccttcc agcaccggaa agggcatggc
     3661 cccgaagtcc agcatgaggt ggtgcgtgtc gtactctgtg ccgacttcgg tgacgcgcac
     3721 gttgccgacc acggtggcgg tgacgaactt gtcggcggcc ttggggccat acagattggc
     3781 gtaggcgtgc gcggcggacc agggcggcgt ggtggcgcca aaggcggatg agttgaaggg
     3841 cgcttcgcca gtcgggtctt gcgcggcggc atccatctgc gcttgcgcca ccaggtcctc
     3901 tgccgaattg gtggcacctt cggccacgcc cagctcggcc agttgctcgg gcgttttctc
     3961 ggccggcagg tcgtcccagc tgaattgctc atcgagcgta tagggctgcg acttgggcac
     4021 gagccgccag ttgtcgatgc tgccggtggg gcatggcgag atgcaatcca tgcagaagtt
     4081 gcacttgctg gcatcgacca cgtagttgcg gctgtcgtgc gtgatggcct gcacggggca
     4141 gatcgattcg caggtgttgc agcggatgca gatctcgggg tcgatcaggt gctgcttgag
     4201 gatcgctgag gtgttcatgg tcaactccta acagccgtac gcgaagaacg cgaaggattc
     4261 gcgaaggagg caaaaggaaa accaaaaatg attttgagtt ctttttcgcg tccttcgcgt
     4321 gatcttcgcg tccttcgcgt ccggtattcc gggtgttcag tcgcgcttca attaaaccgc
     4381 acgtactcga agtcaaccgg ctgccggttg atgcccatga ccggcggcgc aatccagccg
     4441 gcgaacttgc cgggctccac gacgcggccc atcaggctgg ccacatacgc gcggtcttcc
     4501 ggcgtcggca gccactggtc aaccttggcc ttccactccg cctcgctcac cacctgtcct
     4561 tcgggactga ctttcacgcc ggccagcgcg ccgatgttgc ggtggaaggc cttgtgcggc
     4621 actttcagtt tgaaggggat gccggccttg tcgatgatgc ggttccagcg ctcgacgccg
     4681 cccacgctgt ccttgatgta gtcgtcgcgc agcacttcgt tcagcgcgtt gagcatgggc
     4741 acttcttttt cggtcagctt gccgtcttgc acgttgagca ccttgtagct ctggcctttg
     4801 aggacgtgat cgtcctggcg cttgccttct tcgtagcggc ccttcaggcc cgtggagtag
     4861 aaggtggcgg cgttgctcga ttcgtcggcg ccaaacaggt cgatggtgac gctgaagtgg
     4921 aagttgaggt aacgctgaat cgtcggtaag tcgatcacgc ccgccgcgcg cagcttggcc
     4981 gggtcgtcgg tcttgagctg gttcatcatg tccaccgtgc gctgaatgac gcggcccacg
     5041 ccggattcgc cgacgaacat gtggtgcgct tcttcggtca gcatgaactt ggtggtgcgg
     5101 gcgagcgggt cgaagctgct ctcggccagc gcgcacagct ggaacttgcc gtcgcggtcg
     5161 gtgaagtagg tgaacatga
//
LOCUS       DAOMVO010000166         5172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4463_length_5172_cov_12.842290, whole genome shotgun sequence.
ACCESSION   DAOMVO010000166 DAOMVO010000000
VERSION     DAOMVO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_5172_cov_12.842290"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKV30_08740"
     CDS             <1..1098
                     /locus_tag="PKV30_08740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRL30334.1"
                     /translation="GVPGSDIGSVVTGNMAPGDFDQFFLPRHIGLYAGVPVAVPAIMA
                     QRICGTGFELFRQAGEHIQGGACEAALVVGTENMTRNPIAAFDNRTGFKLGAPVGFKD
                     YMWEALKDPAAGINMIQTAENLAKKYGITREQVDEFAAASFAKAVAAQQSGFLAGEIV
                     PVVSEKFELEGYQPRGIKLQGKTTEVAQDTHPRPSPLDVLAKLRPVFEGGVQTGGNSS
                     ALVDAAAAAVVASGAYAKANGKQPLARIVGAAAVGVPPEIMGIGPAPAIRLLLERTDL
                     KLQDIGRFEVNEAQGAQTLSVAKELGMDLARLNVNGGAIALGHPLAATGVRLTITLAR
                     EMQRAGVKYGISSACVGGGQGIALLLENPAA"
     gene            1173..1937
                     /locus_tag="PKV30_08745"
     CDS             1173..1937
                     /locus_tag="PKV30_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL30335.1"
                     /translation="MQINGQAALVTGGGSGLGEATARELARLGAKVAVLDLNLDNAKK
                     VAADINGLAIQCDVSSGDSMQSAIEQASAAHGPARILMQIAGIGSAKRVVGKDGSAAP
                     LEDFIKVVNVNLIGTYNAARLFAAACAKLPPMEDGERGAMVFTASVAAFDGQVGQQAY
                     SASKGGVVGMTLPMARDLAQHGIRVCTIAPGLFSTPLMRTLPQPVQASLAASIPFPPR
                     LGKPEEFAELACHIVTNGHLNGEVIRLDGALRMAPR"
     gene            1951..2370
                     /locus_tag="PKV30_08750"
     CDS             1951..2370
                     /locus_tag="PKV30_08750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL30336.1"
                     /translation="MSKTTVYEVKVMFGDCDPAGIVFFPNFCKWMDASSLDFFMQCGI
                     PPWRELKKTHGIIGTPLLEIHTKFFKPATYGDTLQVHTHVAEWREKVIVQKHVVQRGE
                     LVLCEGLETRAFVVHPDGDPDRLKAIQVPDFIKEKCL"
     gene            2438..3577
                     /locus_tag="PKV30_08755"
     CDS             2438..3577
                     /locus_tag="PKV30_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30337.1"
                     /translation="MTRFTLKTLVAASALAASGAALADITIGVIAPMTGAASGLGIPA
                     GNQAKLWPKTIAGEKLNVIVMDDATDPNQGVTHAKRFVTNDKVDVIMGSVATPVAAAV
                     AQVAEEAATPQIAWSPVAVPAGKDKWLFRLPQSNAVMGHAVIEHMKKQGIKSVGFLGY
                     SDAYGETWLNDFKPQAEKAGIKFGPVERFARADTSVTGQALKLVQANPEAILVVASGS
                     GAAMPQMALAERGYKGKIYQTHAAATRDLMRVGGKAVEGGFVVSGPAVLAEQLPDSHP
                     SKAEAIKFVQQYEKAYGPGSRNQFAGHAYDSIIVMEKAVPIALKKARPGTPEFRAALR
                     DAFEQIGKTTLAHGVLNWTPTDHWGYTDDTGVMMKVTGGEFKVEK"
     gene            3755..4258
                     /locus_tag="PKV30_08760"
     CDS             3755..4258
                     /locus_tag="PKV30_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRL30338.1"
                     /translation="MSPNTETLAASHDAPPATPAAPAIVDAVDTRYLQTLLGYNARRA
                     ALTVIDEFVPRMAQFGLRVVDFSILSVIHHNPGITSRQLCAALNLLPPNLVGKIGGLE
                     SRGLLARRPHPQDGRALGLHLTAEGQALMARAEAEAVALENDAAPGLTRAERRTLIRL
                     LQKVYRP"
     gene            4422..4730
                     /locus_tag="PKV30_08765"
     CDS             4422..4730
                     /locus_tag="PKV30_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRL30339.1"
                     /translation="MSSKENAATGQLLALLEARYAIRVLWALRDGHPQTFRLLQDSVG
                     GITPNTLNTRLKELRAANLVTHGSDGYSLTMSGADLVKRLSDLQAFATKWVAAQAKKK
                     "
     gene            4833..>5172
                     /locus_tag="PKV30_08770"
     CDS             4833..>5172
                     /locus_tag="PKV30_08770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRL30340.1"
                     /translation="MSNGLQITDTKAGTGAEARKGQTVTVHYTGWLYNNGQQGAKFDS
                     SRDRKDPFKFPLGSGMVIKGWDEGVAGMKVGGQRTLIIPPEMGYGARGAGGVIPPNAT
                     LKFDVELLGVD"
BASE COUNT          990 a         1719 c         1671 g          792 t
ORIGIN      
        1 ggcgtgccgg gcagcgacat cggctcggtc gtcaccggca acatggcgcc gggcgacttc
       61 gaccagttct tcctgccgcg ccacatcggc ctgtacgccg gcgtgccggt ggcggtgcct
      121 gccatcatgg cgcagcgcat ctgcggcacg ggcttcgagc tgttccgcca ggcgggcgag
      181 cacatccagg gcggcgcgtg cgaggcggcg ctggtggtgg gcaccgagaa catgacgcgc
      241 aacccgattg ccgccttcga caaccgcacc ggcttcaagc tgggcgcgcc ggtcggcttc
      301 aaggactaca tgtgggaggc gctgaaagac cccgccgccg gcatcaacat gatccagacg
      361 gccgagaacc tggcgaagaa atacggcatc acgcgcgagc aggtcgatga atttgccgcc
      421 gcctcgtttg ccaaggccgt ggcggcgcag caaagcggct ttctggcggg cgagatcgtg
      481 cccgtggtca gcgagaagtt tgagctggag ggctaccagc cgcgcggcat caagctgcag
      541 ggcaaaacca ccgaagtcgc gcaggacacg cacccgcgcc cttccccgct cgacgtgctg
      601 gccaagctgc ggccggtgtt cgaaggcggc gtgcagaccg gtggcaactc gtcggcgttg
      661 gtcgatgccg ctgctgctgc cgtggtggca tcgggcgcgt atgcgaaggc gaacggcaaa
      721 cagccgctgg cacgcatcgt cggcgcggcg gccgtcggcg tgccgcccga gatcatgggc
      781 atcggcccgg cgccggccat tcgtctgctg ctggagcgca ccgacctcaa gcttcaagat
      841 atcggccgct tcgaagtcaa cgaggcacaa ggcgcgcaga cgctgtcggt ggccaaggag
      901 ctgggcatgg acctggccag gctcaacgtc aacggcggcg ccatcgccct cggccacccg
      961 ctggcggcca cgggggtgcg cctgacgatc acgcttgcgc gcgagatgca gcgcgcgggt
     1021 gtgaagtacg gcatctcgtc cgcatgcgtg ggcggcgggc aaggcattgc gctgctgctg
     1081 gagaacccgg cagcatgatc gtttcaatat aaaaacagcc tccagcgctt gatgggcaag
     1141 cgcaagcagc tatcaataca ggagtcatca ccatgcagat caacggacag gcagcgctgg
     1201 tcaccggcgg cggatcgggt ttgggcgagg ccacggcgcg tgaactggcg cgcttgggcg
     1261 ccaaggtggc agtgctcgat ttgaacctcg acaacgcgaa gaaggtcgcg gccgacatca
     1321 acggcctggc gattcaatgc gatgtgagca gcggcgacag catgcaatcg gccatcgaac
     1381 aggcgtcagc cgcgcacggc ccggcgcgca tcctgatgca aatcgccggc atcggctcgg
     1441 ccaagcgcgt ggtgggcaaa gacggcagcg cggcgcctct cgaagatttc atcaaggtcg
     1501 tcaacgtcaa cctgatcggc acctacaacg ccgcgcgcct gttcgccgcc gcgtgcgcga
     1561 agctcccacc gatggaagac ggcgagcgcg gcgccatggt gttcaccgcc agcgtggccg
     1621 cgttcgacgg ccaggtcggc cagcaggcct acagcgcgtc gaaaggcggc gtggtcggca
     1681 tgacgctgcc gatggcgcgc gacctggcgc agcacggcat ccgcgtgtgc accatcgcgc
     1741 cgggcctgtt cagcacgccg ctgatgcgca cgctgcccca acccgtgcaa gcttcgctgg
     1801 ccgcctccat ccccttcccg ccgcgcctgg gcaagcccga agaattcgcc gagctggcct
     1861 gccacatcgt caccaatggg cacctgaacg gcgaggtgat ccgcctagac ggcgcgctgc
     1921 gcatggcacc gcgttaaccc cgacaacacc atgagcaaaa ccaccgttta cgaagtcaag
     1981 gtgatgttcg gcgattgcga tccggccggc atcgtgttct ttcccaactt ctgcaagtgg
     2041 atggatgcgt cgtcgctcga cttcttcatg cagtgtggca ttccgccctg gcgcgagttg
     2101 aagaagacgc acggcatcat cggcacgccg cttttggaga tccacaccaa gttcttcaag
     2161 cccgccacct acggcgacac gctgcaggtg cacacgcacg tggcggaatg gcgcgagaag
     2221 gtcatcgtgc aaaagcacgt cgtgcagcgc ggcgagctgg tgctgtgcga ggggctggag
     2281 acgcgcgcct tcgtcgtgca ccccgatggg gacccggatc ggctgaaggc cattcaggtg
     2341 cccgatttca tcaaggaaaa gtgcctctag cgcttgattg gcaagcgcca gcagctatca
     2401 aaaccatatt cgtcattcac caccaggaga ccgacccatg acccgcttca ccctcaagac
     2461 cctcgtggcc gccagcgccc tcgccgcctc gggcgccgcg ctggccgaca tcaccatcgg
     2521 cgtcatcgcg ccgatgacgg gcgccgcatc gggcctcggc attccggccg gcaaccaggc
     2581 caagctgtgg cccaagacca ttgccggcga aaagctcaac gtcatcgtga tggacgacgc
     2641 caccgacccc aaccagggcg tgacgcacgc caagcgcttt gtcaccaacg acaaggtcga
     2701 cgtcatcatg ggctcggtcg ccacgccggt ggccgccgcc gtcgcgcagg tggctgaaga
     2761 agctgctaca ccgcagatcg cctggtcgcc cgtcgccgtg ccggcgggca aggacaagtg
     2821 gctgttccgc ctgccgcaat ccaacgccgt gatgggccac gccgtcatcg agcacatgaa
     2881 gaagcagggc atcaagagcg tcggctttct gggctacagc gatgcttacg gtgaaacttg
     2941 gctcaacgac ttcaagccac aggccgaaaa ggccggcatc aagttcggcc cggtcgagcg
     3001 ctttgcccgc gccgacacca gcgtgacggg ccaggcgctc aagctggtgc aggccaaccc
     3061 ggaggcgatc ctggtcgtcg cctccggctc gggcgcggcg atgccgcaga tggcgctcgc
     3121 cgagcgcggc tacaagggca agatctacca gacccacgcc gccgccacgc gcgacttgat
     3181 gcgcgtgggc ggcaaggcgg tggaaggcgg ctttgtcgtc agcggcccgg cggtgctggc
     3241 cgagcagttg ccggattcgc atccctcgaa ggccgaggcg atcaagttcg tgcagcagta
     3301 cgaaaaagcc tacggccccg gatcgcgcaa ccagtttgcc ggccacgcct acgactcgat
     3361 catcgtgatg gaaaaggcgg tgccgatcgc gctgaagaaa gccaggcccg gcacgcccga
     3421 attccgcgcc gcgctgcgcg acgccttcga gcagatcggc aagaccaccc tcgcccacgg
     3481 cgtgctgaac tggacgccca ccgaccactg gggctacacc gacgacaccg gcgtgatgat
     3541 gaaagtcacg ggcggcgagt tcaaggtcga gaagtaagcg gactggcaga catcctgcca
     3601 ttcagcaccg ttcgtcctga gcctgtcgaa ggactgcttg ccatgggagc aggcttcggc
     3661 gagcctgtca tctatagaac aggccactgg cgcaagccag acaagcgctg gcagctatat
     3721 aaataggagt aatgactcaa tcgtcgcctg tcccatgagc cccaacaccg aaaccctcgc
     3781 tgcgtcgcac gacgccccac ctgccacacc tgccgcacct gccatcgtcg acgcggtcga
     3841 cacccgctac ctgcaaaccc tgttgggcta caacgcccgt cgcgctgcgc tcacggtgat
     3901 cgacgaattc gtgccgcgca tggcacagtt cggcctgcgc gtggttgatt tttcgatact
     3961 ttccgtgatt caccacaatc cgggcatcac ctcgcggcag ttgtgcgcgg ctctcaacct
     4021 gctgccgccc aacctggtcg gcaagatcgg cgggctggaa tcacgcggcc tgctggcgcg
     4081 ccggccgcac ccgcaggacg gccgcgcgct cggcctgcac ctgacggccg agggccaggc
     4141 gctgatggcg cgcgccgaag ccgaggccgt ggcgcttgaa aacgacgccg ccccgggcct
     4201 gacgcgcgcc gagcgccgca cgctgattcg gctgctgcaa aaagtctatc ggccctgacc
     4261 gcgggtttac cccagatgcg ccggggcgcg ggtaaatcag tattttgtag cggcaaaccc
     4321 acggcggggc ggcgcgggga gctatgaaac tgctagcgtg ctagcaaatt tgcgctatag
     4381 tcagcttcac gcaccttagc gaacttgagg agaagcctga catgagttca aaagaaaacg
     4441 ccgccacagg ccagttgctg gcgctgcttg aagcgcgcta cgccatccgc gtgctgtggg
     4501 cgctgcgcga cggccatccc cagactttcc gcctgctgca agacagcgtc ggcggcatca
     4561 cgcccaacac gctgaacacg cgcctgaagg agctccgcgc ggccaacctg gttacgcacg
     4621 gctccgacgg ctacagcctg accatgagcg gcgccgacct ggtcaagcgt ctgtccgatc
     4681 tgcaggcctt tgcgactaag tgggtggcgg cacaggccaa gaagaagtaa gccgcgcggc
     4741 acggcgcgac ctggcgtgcc attcatcgtc aaaaacactt ccagcgcttg ccagacaagc
     4801 gcgggcagct acataaaaca tagtgcaaaa gcatgagcaa cggattgcag atcaccgaca
     4861 ccaaggccgg cacgggcgcc gaggctcgca agggccagac cgtcacggtg cattacaccg
     4921 gctggctcta caacaacggc cagcaaggcg ccaagttcga ttccagccgc gaccgcaagg
     4981 atccgttcaa gtttccgctg ggttccggca tggtcatcaa gggctgggac gaaggcgtgg
     5041 ccggcatgaa agtcggcggt cagcgcacgc tgatcattcc gcccgagatg ggctacggcg
     5101 cgcgcggcgc tggcggtgtg attccgccca atgccacgct gaaattcgat gtggagttgc
     5161 tgggcgtcga ct
//
LOCUS       DAOMVO010000167         5130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4518_length_5130_cov_12.767882, whole genome shotgun sequence.
ACCESSION   DAOMVO010000167 DAOMVO010000000
VERSION     DAOMVO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_5130_cov_12.767882"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKV30_08775"
     CDS             complement(<1..644)
                     /locus_tag="PKV30_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRL30341.1"
                     /translation="MQDHSKPQAGFSYSRAFFEELVDTAMAHAKKLGASDVGAEASEG
                     SGLSVSVRKGAMETVERNRDKSLGVTVYLGQRRGNASTSDFSRAAIEQTVQAAYDIAR
                     FTAADPVAGLPDEADIARPAEQRDLDLFHPWPITSEQAAEMALVCEAAAFDVDKRIAN
                     SEGAGVSAQQSHFFSAHTHGFRGGYASSRHSISVAPIAGKGNGMQRDYWYSSER"
     gene            816..1511
                     /gene="yjgA"
                     /locus_tag="PKV30_08780"
     CDS             816..1511
                     /gene="yjgA"
                     /locus_tag="PKV30_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HRL30342.1"
                     /translation="MSRKPKKGYFVRGHFVAEGSELDLELKRELKGGDAPSKTELKAQ
                     SAELQALGEALLELRRDLFEPLDLPAKLIDALSELKRITNFEGKRRQAQLVGKLMRQL
                     EDEQIDAVRAALEVQRKGSAADTLRLHAAENWRDRLIAEDAAVNAWVTQYPDTDVQQL
                     RALVRQARKDVPAPTDARVAEATGQAPRQGRAYRELFQLVRDALTRAEASTPQVQAID
                     AEDAGYTDDSRAG"
     gene            1629..2222
                     /gene="mog"
                     /locus_tag="PKV30_08785"
     CDS             1629..2222
                     /gene="mog"
                     /locus_tag="PKV30_08785"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRL30343.1"
                     /translation="MNSPDPVTIGIVSISDRASSGAYEDKGLPALQDWMGKALKNPIT
                     FAPRLIPDEQATISRTLIELVDAGCALVLTTGGTGPAVRDVTPEATLAVADKEMPGFG
                     EQMRQISLAFVPTAILSRQVAVIRKQSLIINLPGQPKAIAETLEGLKAADGTQKVHGI
                     FAAVPYCIDLIGGPYLETDEAVCKAFRPKSAVRASNA"
     gene            2285..3298
                     /locus_tag="PKV30_08790"
     CDS             2285..3298
                     /locus_tag="PKV30_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30344.1"
                     /translation="MSIYRCNGCGFVSEESTTPVGQKISCSRCGTPSTVYGTVFFVEK
                     LVERYAAAMRELQALRAADDAPESNPGNDTAGNEPAGAAALMDAADYNTRNFATAEQH
                     APLQAWFAARRIEAQFDYAQVDTTGFFDDAARLLGDRIDLFGELIDRVCYAYRKSHGW
                     LNLELARLAQKDAQAINQLCRQLYSHTFFARYHYQKPEKIVRLTLQTAPAVRRFFEGG
                     WLEWYVFIEALEAMKAASRANVSCARGVKVVFPNEDLHELDVMVLPPGQSPICIECKS
                     GEFRRDIDKYLRLRKRLGLERQRFIVCAADLSDEQAAALSAMYELTFVNLRSLKSHLQ
                     ALN"
     assembly_gap    3309..3408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3409..3591
                     /locus_tag="PKV30_08795"
     CDS             <3409..3591
                     /locus_tag="PKV30_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30345.1"
                     /translation="SVSAKVREFLARYARGENGDAADSAAGAVKLPVFNGGQGLAPGL
                     DPLSNKSLRAAAEEDA"
     gene            3588..4010
                     /locus_tag="PKV30_08800"
     CDS             3588..4010
                     /locus_tag="PKV30_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL30346.1"
                     /translation="MTPDVNVLVAASRADHPHHATARDWLESVLADRGRLTLLPTVTA
                     SFVRLVTHPKVFVEPTPIDAALDFIEVVRQAAAVSGRTPDEWPQLKDLCQRHQLRAND
                     LPDAWIAAQVQVRGLVLVTFDRDLVKLLPAERLQLLSA"
     gene            complement(4025..4795)
                     /gene="cysE"
                     /locus_tag="PKV30_08805"
     CDS             complement(4025..4795)
                     /gene="cysE"
                     /locus_tag="PKV30_08805"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRL30347.1"
                     /translation="MLHRIRSYTQAILERDPAARSVWDVVFSYPGFHALVLHRIAHAL
                     WVRGWFWLARWISHWARFFTGIEIHPGAKIGERVFIDHGMGVVIGETAEIGDDCTIYQ
                     GVTLGGTSLGKGTKRHPTLGKGVVVGASSQVLGGFTVGDGAKIGSSAVVTKPVPPGAT
                     AVGNPARIILADDVAKREEAAAKLGFSAYGVTQSDDPVSQALRGLIDGTATHDHQITL
                     LWQAVEKLACKLDPDCVPKEAAKEETFEAEKWNEVVGK"
     gene            complement(4810..>5130)
                     /locus_tag="PKV30_08810"
     CDS             complement(4810..>5130)
                     /locus_tag="PKV30_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRL30348.1"
                     /translation="YGSLNIAAAVQLIAYDWRLALAARFGGFAIGPAAREPVRADAPQ
                     MAGMLAHWQQALVDIDFLDPAAPKKLMPRLQQLFNRAQPTVEEIHILRGIAKAMQAAA
                     ERRR"
BASE COUNT          882 a         1652 c         1644 g          852 t
ORIGIN      
        1 ttgcgctctg agctgtacca gtaatcgcgc tgcatgccat tgcccttgcc ggcaatcggc
       61 gccaccgaaa tgctgtggcg cgacgacgca tagccgccgc gaaaaccgtg cgtgtgggcg
      121 ctgaaaaaat ggctttgttg cgccgacacg cccgcgcctt cgctgttggc gatgcgcttg
      181 tccacatcaa acgccgccgc ctcgcacacc agcgccatct cggccgcctg ctcgctggtg
      241 atcggccagg ggtggaacaa gtccaggtcg cgctgctcgg cggggcgggc gatgtcggcc
      301 tcatccggca ggccagcgac cggatcggcg gcggtgaagc gggcaatgtc gtaggccgcc
      361 tgcaccgtct gctcgatggc ggcgcgcgaa aaatccgacg tgctggcgtt gccgcgccgc
      421 tggcccaggt agaccgtcac gccgagcgac ttgtcgcggt tgcgctccac cgtctccatc
      481 gcgcccttgc gcacgctgac actgaggccc gagccttcgg acgcctcggc gcccacgtcg
      541 ctggcgccca gcttcttggc gtgcgccatg gcggtgtcga ccagctcttc gaagaatgcg
      601 cgcgaataag aaaagcccgc ttggggcttg gagtgatctt gcatggtgac ggctattcta
      661 ggcgtcggct cgcgccatgg cggtgcccgt cgcaagcggt cgctttcgcg ccgcgcttga
      721 cgggtaaaat cgggctgcag cgcacgcctg aaaagcgcga gcagctacaa aaataaaagc
      781 gcactgcgtc gccgccacct accctgaacc acaccatgtc ccgcaaaccc aagaaaggct
      841 atttcgtgcg cggccacttc gttgccgaag gcagcgaact ggacctggag ctcaagcgcg
      901 aactgaaggg cggcgacgcg cccagcaaga ccgagttgaa ggcgcaaagc gccgaacttc
      961 aggcgctggg cgaagccctg ctggagctgc ggcgcgattt gttcgagccg ctcgatctgc
     1021 cggccaagct gatcgacgcg ctgtccgagt tgaagcgcat caccaacttc gagggcaagc
     1081 gccgccaggc gcagctggtc ggcaagctga tgcgccagct ggaagacgag cagatcgacg
     1141 ccgtgcgcgc cgcgctcgaa gtgcagcgca agggctcggc cgccgacacg ctgcgcctgc
     1201 acgcggccga aaattggcgc gaccgcctga tcgccgaaga cgccgccgtg aacgcctggg
     1261 tcacgcaata ccccgacacc gacgtgcagc agttgcgcgc gctggtgcgc caggcgcgca
     1321 aggacgtgcc cgctcccacc gacgcccgcg tggccgaagc caccggccag gcgccgcgcc
     1381 agggccgcgc gtaccgcgag ctgtttcagc tggtgcgcga cgcgctgacc cgcgccgaag
     1441 ccagcacgcc gcaggtgcaa gccatcgacg ccgaagacgc gggctacacc gacgacagcc
     1501 gggccggctg acgcatgaca gaatgacacc gccgctggcg cggccgcatg aaggcgctgc
     1561 gcgccatgac ctccgcctcc tttcgtcatt gacaccgcgt cagcggtgaa gtcatttcgt
     1621 catccgccat gaactcaccc gaccccgtca ccatcggcat cgtctccatc agcgaccgcg
     1681 cgtccagtgg cgcctatgaa gacaagggcc tgcccgcgct gcaagactgg atgggcaagg
     1741 cgctgaaaaa ccccatcacc ttcgcgccgc gcctgatccc cgacgagcag gccaccatca
     1801 gtcgaacgct gatcgagctg gtcgatgccg gctgcgcgct ggtgctgacc accggcggca
     1861 ccggccccgc cgtgcgcgac gtcacgcccg aagccacgct ggccgtggcc gacaaggaaa
     1921 tgcccggctt tggcgagcaa atgcgccaga tcagcctggc cttcgtgccc accgccatcc
     1981 tgtcgcgcca ggtggccgtg attcgcaagc agagtctgat catcaacctg cccgggcagc
     2041 cgaaggcgat tgccgagacg ctggaggggt tgaaggctgc cgacggcacg caaaaagtgc
     2101 acggcatctt tgccgccgtg ccctactgca tcgatctgat cggcggcccg tatctggaaa
     2161 cggatgaggc ggtgtgcaag gcgttcaggc caaagagcgc ggtgcgggca agtaacgctt
     2221 gacgcccagg cactgcactg tctgcgtaca accgtcgcca atttccaaga agagccaaaa
     2281 ataaatgtcg atctatcgct gcaatggctg cgggttcgtc tcggaggagt cgacgacgcc
     2341 cgtgggtcag aagatctctt gcagtcgctg tggcacgccg agcaccgtct acggaactgt
     2401 gtttttcgtc gagaagcttg ttgaacgcta cgcggccgcc atgcgtgagt tgcaggcctt
     2461 gcgcgctgcc gatgacgcgc cagaaagcaa ccccggcaat gacactgccg gcaacgagcc
     2521 ggccggcgcc gcggccctga tggatgctgc ggactacaac acgcgcaact ttgccaccgc
     2581 cgagcagcac gccccgcttc aggcctggtt tgccgcccgt cgcatcgaag cgcagttcga
     2641 ctacgcgcag gtagacacga ccggcttctt cgacgatgcg gcgcgcttgt taggcgaccg
     2701 aatcgatttg ttcggtgaac tgatcgaccg ggtgtgctat gcctatcgca agagccacgg
     2761 ttggctgaat cttgagttgg cgcgccttgc acaaaaggac gcgcaagcca tcaaccagct
     2821 ttgccgccag ctttacagcc ataccttctt cgcgcgctat cactaccaga aacccgagaa
     2881 gattgtgcgt ctgacgctcc aaacagctcc ggctgtgcgg cggtttttcg agggcggttg
     2941 gcttgagtgg tacgtgttca tcgaggctct ggaagcgatg aaggccgcca gccgcgcgaa
     3001 cgtgtcctgc gcgcgcggcg tcaaggtggt gtttccgaac gaagacctgc acgagttgga
     3061 tgtgatggtg ttgccgccgg gtcaatcgcc catctgcatc gagtgcaaga gcggagagtt
     3121 tcgtcgcgac atcgacaagt acctgcgact gcgcaaacgt ctgggccttg agcggcagcg
     3181 cttcatcgtc tgcgcagcag acctgagcga tgagcaggcc gccgcgctgt cggcgatgta
     3241 tgagctgacc tttgtcaatc tgcgctcatt gaagtcgcat cttcaggcgc tgaactgaat
     3301 tcgcctgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ggtcagcgcc
     3421 aaggtgcgcg agtttctggc gcgctatgca cggggcgaga acggtgacgc tgccgactct
     3481 gcggcgggcg cggtgaagct tccggtattc aacggtggtc agggactggc gccgggcctc
     3541 gatcccttga gcaacaaatc actgcgcgcc gccgccgaag aagacgcgtg acgcccgacg
     3601 ttaatgtgtt ggtggcggca tcgcgcgccg accatccgca ccacgccacg gcgcgcgact
     3661 ggcttgaatc ggtcttggcg gaccggggcc gactgacttt gttgcccacg gtcaccgcca
     3721 gctttgtaag gctggtcaca cacccgaagg tgtttgtcga gccgacgccc atcgatgcgg
     3781 cgctggattt catcgaagtg gtcaggcaag cagcagccgt gtccggtcgc acacccgacg
     3841 aatggccgca gttgaaggac ttgtgccagc gccaccaact gcgcgccaac gacctgcccg
     3901 atgcgtggat cgccgcgcaa gtgcaggtgc gcgggttggt gttggtcact ttcgaccgcg
     3961 atcttgtgaa gctgttgccg gccgagcggc tgcaattgct atcggcgtga agccggtggt
     4021 cggctcactt ccccaccacc tcgttccact tctccgcctc gaaggtctct tccttcgcgg
     4081 cctctttcgg cacgcagtcc gggtcgagct tgcaggccag tttttcaacc gcctgccaca
     4141 gcagcgtgat ctggtggtcg tgcgtggcgg tgccgtcgat caggccgcgc agcgcctggc
     4201 tgacggggtc gtcgctttgc gtgacgccgt aggccgaaaa acccagcttg gccgccgctt
     4261 cttcgcgctt ggccacgtcg tcggcgagga tgatgcgtgc cgggttgccc accgccgtgg
     4321 cgccgggcgg cacgggtttg gtgaccaccg ccgatgaacc gatcttggcc ccatcgccca
     4381 ccgtgaagcc gcccagcacc tgactgctgg cgccgacaac gacgcccttg cccaatgtgg
     4441 ggtggcgctt ggttcctttg ccgagcgagg tgccgccgag cgtgacgccc tggtaaatgg
     4501 tgcagtcgtc gccgatttcg gccgtctcgc cgatcaccac gcccatgccg tggtcgatga
     4561 agacgcgctc gcctattttt gcgccggggt ggatctcgat gccggtgaaa aagcgcgccc
     4621 agtgcgagat ccagcgcgcc agccagaacc agccgcgcac ccacagcgcg tgggcgatgc
     4681 ggtgcagcac cagcgcgtga aaaccggggt aggaaaacac cacgtcccac accgagcgcg
     4741 cggcggggtc gcgctcgaga atggcctggg tgtaggagcg gatgcggtgc agcatggtgg
     4801 gcggcaagtc tagcgtctgc gctcggccgc tgcctgcatc gccttggcaa tgccacgcag
     4861 gatgtggatt tcctccaccg tcggctgcgc acggttgaac aactgctgca ggcgcggcat
     4921 cagcttcttg ggcgcggcgg ggtcgagaaa gtcgatgtcg accagcgcct gctgccagtg
     4981 ggcgagcatg ccggccattt gcggcgcgtc ggcccgcacg ggctcgcgcg cggcgggccc
     5041 gatggcgaag ccgccaaagc gcgcggccag cgccaggcgc cagtcgtagg cgatcagctg
     5101 cacggcggcg gcgatgttga gcgagccgta
//
LOCUS       DAOMVO010000168         5126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4525_length_5126_cov_14.913824, whole genome shotgun sequence.
ACCESSION   DAOMVO010000168 DAOMVO010000000
VERSION     DAOMVO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4525_length_5126_cov_14.913824"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKV30_08815"
     CDS             <1..210
                     /locus_tag="PKV30_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiH"
                     /protein_id="HRL30349.1"
                     /translation="SPGDLKLLRRYERARQGDWLRMSLATDGLQQLFGRPEPVAALLR
                     NWGMAAFDASGPLKRRIARLAMGTA"
     gene            263..979
                     /locus_tag="PKV30_08820"
     CDS             263..979
                     /locus_tag="PKV30_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HRL30350.1"
                     /translation="MKLSTFARTGLTALVLALPFVAAAQEATLRKNLAERVPGLSDID
                     EVRKTPMNGLYEVRVGTEIFYTDAEGNYLLHGNLLDTKARKNLTEERIEKLTAIDFKE
                     LNVKNAFTMVRGNGKRKIAVFEDPNCGYCKRFEKDLAKINNVTVHFFLIPILGADSVE
                     KSRQIWCSADKVKTWNDWMQRDVTPKGAGSCNTDALTANLDFAKKYRITGTPTLVFEG
                     GQRVPGAINAQQIEELLAKN"
     gene            1016..1495
                     /locus_tag="PKV30_08825"
     CDS             1016..1495
                     /locus_tag="PKV30_08825"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HRL30351.1"
                     /translation="MTEPDRLTLADRQPPRWRLIYLVAYGSLVPLAALALLLWWPQAP
                     ARAALGLALTSYSAMLASFIGGFHWGIGLRYMATTSEMPTFHFVWGPVPAYLAWGAFL
                     LQPPLSLVLLAAVLVLAYLVDARTWPGAGLGPWLPLRRQVTMVAVACCLLGTGALWP"
     gene            1503..3317
                     /locus_tag="PKV30_08830"
     CDS             1503..3317
                     /locus_tag="PKV30_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HRL30352.1"
                     /translation="MSTSPARTRRKPAAAPAPAVRYDVRMASLGGHRFGVQIRIEQPA
                     ATQRVSLPAWIPGSYLIREFARHLLPLHAAQARTERACTQIDKSTWDIDCDPRRPLVL
                     RYEVYAFDNSVRTAWLDSERGFFNGSSLLLRVHGHEEAPHELTVHAPRERRDWQLATA
                     MTPVDIDAQGFGRYHAADYAELIDHPVELGAFWRGDFTAGGVPHRFVVAGAPASFDGA
                     RLLRDAQKICEAAIRFWHGAGKPPFKHYLFMLNAVGEGYGGLEHRASTALICGRRDLP
                     RHDSPASAARVGDGYVTLLGLISHEYFHAWNVKRLRPAEFTRYEYQSENYTRLLWFFE
                     GFTSYYDDLLLRRAGLIDDAAYLKLLTKTINQVLQAPGRHVQSVAQASFDAWVRYYRP
                     DENTPNSTISYYTKGALVALCFDLTLRAEGRTTLGDVMRALWRRCAGGPMTEGDFADT
                     LAELAGRRFDTEIADWVHGTGELPLRALLEAQGVRVLEEPSQLAQTLGLRVAEDGGIT
                     VKVVLDGGPAADAGLAAGDEWLGVELPAEKPGTPVAWRLTRLDELPLYAGAARRVTAL
                     VARDKRLLRLPLALPEKSTTWRLAPTDSSTEAPTWPGR"
     assembly_gap    3492..3591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3592..4290
                     /locus_tag="PKV30_08835"
     CDS             <3592..4290
                     /locus_tag="PKV30_08835"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRL30353.1"
                     /translation="VAAASAAPAASSPGPGDAPFLASWPSDTRLTYKLGGNYRGELHG
                     SARVLWQREGTRYHTAVEMSAGLLASLSFTSQGDITEGGLRPEVYEENVRGRRRGVRL
                     GEDVRLNNGDRIARPEAVQDTASQFVELGHRFSTGQVKLVPGAQVNFSMARPGGIDEW
                     TYDVIGEETLHLPRLGPVPTLHLKPRPLSKPRGPISAEIWYAPSLQYLPVRIRITQGN
                     DNYIDLMVDTIEQR"
     gene            complement(4395..4808)
                     /locus_tag="PKV30_08840"
     CDS             complement(4395..4808)
                     /locus_tag="PKV30_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRL30354.1"
                     /translation="MNTLPVRLSPGDDLRRALEAQAPALGGSAVVVAGIGSLTDARLR
                     FAGAESLVTLPGLFEIISLSGTLSRDGAHLHMSVAGADGRLFGGHVPYGNTVRTTAEV
                     LLVGLHEWQLTRAPDAATGFLELRIEPRSPSGRRG"
     gene            complement(4912..>5126)
                     /locus_tag="PKV30_08845"
     CDS             complement(4912..>5126)
                     /locus_tag="PKV30_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRL30355.1"
                     /translation="REGPMDNLKVAVECAPGVDPAGIAARAAAKLLQHEIKVYVGSSV
                     EIELKGEGGIERSVGKAKRVVDLRKK"
BASE COUNT          809 a         1777 c         1663 g          777 t
ORIGIN      
        1 tcgccgggcg atttgaagct gctgcgccgc tacgagcgcg cgcgccaggg cgactggctg
       61 cgcatgagcc tggccaccga tggcctgcag caactgtttg gccggcccga acccgtcgcc
      121 gcgctgctgc gcaactgggg catggccgcg ttcgacgcca gcggcccgct caagcggcgc
      181 attgcgcggc tggccatggg aactgcgtga gccccagcgg gctcagactg ctttatttca
      241 atttcaaact acaaccgttc ccatgaaact cagcacattc gcgcgcactg gcctcaccgc
      301 cctggttctg gcgctgccct tcgtcgccgc cgcgcaggaa gccacgctgc gcaagaacct
      361 ggccgagcgc gtgcccggcc tgtccgacat cgacgaggtg cgcaagacgc cgatgaatgg
      421 tctgtatgaa gtgcgcgtgg gcaccgaaat tttctacacc gatgccgagg gcaactacct
      481 gctgcacggc aacctgctgg acaccaaggc acgcaagaac ctgaccgagg agcgcatcga
      541 aaagctcacc gccatcgact tcaaggaact caacgtcaag aacgccttca ccatggtgcg
      601 cggcaacggc aagcgcaaga tcgcggtgtt tgaagacccg aactgcggct actgcaagcg
      661 cttcgagaag gacctggcca agatcaacaa cgtcacggtg cacttcttcc tgatcccgat
      721 cctgggcgcc gactcggttg aaaagtcgcg ccagatctgg tgcagcgccg acaaggtcaa
      781 gacctggaac gactggatgc agcgcgacgt gacgcccaag ggcgccggca gctgcaacac
      841 cgacgcactg acggccaacc tggacttcgc caagaagtac cgcatcaccg gcacgccgac
      901 gctggtgttc gagggcggac agcgcgtgcc gggcgccatc aacgcgcagc agatcgagga
      961 actgctggcc aagaactgat cgggtgcttc gagttggccg cgcacaagcc ctgccgtgac
     1021 cgagcccgat cgactgactt tggccgaccg gcagccgccg cgctggcggc tgatctacct
     1081 cgtcgcctac ggctcgctgg tgcccttggc ggccttggcg ctgctgctgt ggtggcctca
     1141 ggcgccggcg cgcgctgccc tcggtctggc gctgaccagc tacagcgcca tgctggcatc
     1201 gttcatcggc ggatttcact ggggcattgg cctgcgctac atggccacca cgtcggagat
     1261 gccgaccttc cactttgtct gggggccggt gccggcctac ctggcgtggg gcgcgttctt
     1321 gctgcagccg ccgctgtcgc tggtgctgct ggcggccgtg ctcgtgctgg cgtatctggt
     1381 cgatgcgcga acctggcccg gcgccgggct cgggccctgg ttgccgctgc ggcgccaggt
     1441 gacgatggtc gccgtggcct gctgcctgct gggcacgggc gccctgtggc catgacgcgc
     1501 gcatgagcac atcgcccgcc cgaacccgcc gcaaaccagc cgcggcaccc gcgccagccg
     1561 tgcgctacga cgtgcgcatg gcgagcctgg gtggccacag gtttggcgtg cagatccgca
     1621 tcgagcagcc cgccgccacg cagcgcgtgt cgctgccggc gtggattccc ggcagctacc
     1681 tgatccgcga attcgcgcgc cacctgctgc ccctgcacgc cgcgcaagcc cgcaccgagc
     1741 gcgcctgcac gcaaatcgac aaaagcacct gggacattga ttgcgacccg cgccggccac
     1801 tggtgctgcg ctacgaggtc tatgccttcg acaactcggt gcgcaccgcc tggctggaca
     1861 gcgagcgcgg cttcttcaac ggcagcagcc tgctgttgcg cgtgcacggg cacgaagagg
     1921 cgccgcatga gctgacggtg cacgcgccgc gcgagcggcg cgactggcaa ctggccacgg
     1981 ccatgacgcc ggtcgacatc gatgcgcaag gctttgggcg ctaccacgcg gcggattacg
     2041 ccgagttgat cgatcacccg gtcgagctgg gcgcgttctg gcgcggtgac ttcaccgctg
     2101 gcggcgtgcc gcaccgcttc gtcgtcgccg gtgcgcccgc atcgttcgac ggcgcgcgcc
     2161 tgctgcgcga tgcgcagaag atttgcgagg ccgccatccg cttctggcac ggcgccggca
     2221 agccgccctt caagcactac ctgttcatgc tgaacgcggt gggcgaaggc tacggcgggc
     2281 tggagcaccg cgccagcacc gcgctgatct gtgggcggcg cgacctgccg cgccacgatt
     2341 cgcccgccag cgccgcgcgc gtgggcgacg gctacgtaac gctgctgggc ctcatcagcc
     2401 acgaatactt tcacgcctgg aacgtcaagc gacttcgccc cgccgaattc acccgctacg
     2461 aataccagag cgaaaactac acgcgcctgc tgtggttctt cgagggcttt accagctact
     2521 acgacgattt gctgctgcgc cgcgcgggtc tgatcgacga cgcggcctac ctcaagctgc
     2581 tgaccaagac catcaaccag gtgctgcagg cgccggggcg ccacgtgcag tcggtggcgc
     2641 aggccagctt tgacgcctgg gtgcgctact accgccccga cgagaacacg cccaacagca
     2701 ccatcagcta ctacaccaag ggcgcgctgg tggcgctgtg ctttgacctg acgctgcgcg
     2761 ccgaaggccg caccacgctg ggcgacgtga tgcgcgcgct gtggcggcgc tgcgctggcg
     2821 gcccgatgac cgagggcgac tttgccgaca cgctggccga actggccggc cgccgcttcg
     2881 acaccgagat cgccgactgg gtgcatggca ccggcgagct gccgctgcgc gcgctgctcg
     2941 aagcgcaagg cgtgcgcgtg cttgaagaac cctcgcaatt ggcgcagacg ctcggcctgc
     3001 gcgtggccga agacggcggc atcacggtga aagtggtgct ggacggcggc cccgccgccg
     3061 acgccggcct tgctgcgggc gacgaatggc tgggcgtgga actgccggca gaaaagccgg
     3121 gtacgcccgt cgcctggcgc ctgacgcggc tggacgagct gcccctgtac gccggcgccg
     3181 cgcgccgcgt caccgcgctg gtggcgcgcg acaagcgcct gctgcgcctg cccctggcgc
     3241 tgcccgagaa atcgaccaca tggcggctgg ccccgacaga cagcagcacc gaagcgccca
     3301 cctggcccgg ccgctgaagc cacgccgggc gctggcggtg accaccgtgc tggtgttgct
     3361 gctgcacgcg ctggcgctgg ccttgctctc gtcgctgctg cgcccgccca gcctgctgaa
     3421 gaccatggcg acgccgttct acacgcgcag cattgcgccc gaagtgccgg cagtggtggc
     3481 agcggcgcca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcgccgcc
     3601 gcatccgccg cgcccgcggc gtcctcgccc ggccctggcg atgcgccttt tctggccagc
     3661 tggccgagcg acacccgcct gacctacaag ctgggcggca actaccgggg cgagctgcac
     3721 ggcagcgcac gcgtgctgtg gcagcgcgaa ggcacgcgct accacacggc ggtggaaatg
     3781 agcgccggct tgctggcttc gctaagcttc accagccagg gcgacatcac cgagggcggc
     3841 ctgaggcccg aggtgtacga agagaacgtg cgcggccgcc ggcgcggcgt gcggctgggc
     3901 gaagacgtgc gcctgaacaa cggcgaccgc atcgcccgcc ccgaggccgt gcaggacacg
     3961 gccagccagt tcgtcgagct gggccaccgt ttctcgaccg gccaggtcaa gctggtgccc
     4021 ggtgcgcagg tcaacttttc gatggcgcgc cctggcggca tcgacgagtg gacctacgac
     4081 gtgattggcg aagaaacgct gcacctaccg cgcctggggc ccgtgcccac cctgcacctc
     4141 aagccgcgcc ccttgagcaa gccacgcggc cccatcagcg ccgagatctg gtacgcgccc
     4201 agcctgcagt atctgccggt gcgcatccgc atcacgcaag gcaacgacaa ctacatcgac
     4261 ctgatggtgg acaccatcga gcagcgatga tgcttccata tcgatagctg ccagcgcttg
     4321 ccagacaagc gcgggaaccc aaaatcgcca tttcggtgca gtggcccgat cgcgcagcgc
     4381 tcgcaacgcg cccttcagcc gcggcggccg gagggcgatc gtggctcgat ccgcagttcc
     4441 agaaatccgg tggcggcatc gggcgcgcgg gtcagctgcc attcgtgcag gccgaccagc
     4501 agcacctcgg ccgtggtgcg cacggtgttg ccgtaaggaa cgtgcccgcc aaagaggcgg
     4561 ccgtcggcac ccgccacgct catgtgtagg tgcgcgccgt cgcgcgacag cgtgcccgac
     4621 aggctgatga tctcgaacag ccccggcagc gtgaccagcg attcggcgcc ggcaaatcgc
     4681 aggcgcgcgt ccgtcaggct gccgatgccc gcgaccacga ccgccgagcc gcccagcgcc
     4741 ggggcctgcg cctccagcgc gcgccgcagg tcgtcgcctg gcgaaagtcg aacgggcaag
     4801 gtgttcacga ggggtgctgt cgcgtgcaag gtcgcatgcg ttttgctacg ctataaatag
     4861 ctgcttgcgc tttcgtatca agcgctggag gcctttttta atccaaaccc tttatttctt
     4921 gcgcagatcc accacgcgct tggccttgcc cacgctgcgc tcgatgccgc cttcgccctt
     4981 caactcgatc tcgacgctgc tgccgacgta gaccttgatc tcgtgctgca gcagcttggc
     5041 ggcggcgcgc gcggcgatgc cggcggggtc aacgcccggc gcgcattcca ccgccacctt
     5101 caggttgtcc atcgggcctt cgcgcg
//
LOCUS       DAOMVO010000169         5106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4558_length_5106_cov_16.474362, whole genome shotgun sequence.
ACCESSION   DAOMVO010000169 DAOMVO010000000
VERSION     DAOMVO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4558_length_5106_cov_16.474362"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..872
                     /locus_tag="PKV30_08850"
     CDS             273..872
                     /locus_tag="PKV30_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HRL30356.1"
                     /translation="MCPSHPRFHHPERRLLSGFLGLLLSVLAGGCATTTTTQMAPDVI
                     AIEEASPGPQPEAAQFIPVVRYGRYTLVELAPMAAQRDLLLQTVDVSMPEDARATVGD
                     GLRHVLKRSGYQLCETAHAVIELYTLPLPAAHLHLGPMSLRDALLTLAGPAWELHADD
                     RTRQVCFEQIGEPTGGAPILEPPATAAAAQPFPLAGGRP"
     gene            869..1516
                     /locus_tag="PKV30_08855"
     CDS             869..1516
                     /locus_tag="PKV30_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30357.1"
                     /translation="MNTSQSSRRPTAAVVVQSLLWLWLICLSVLVVLGYQTMSDLADQ
                     ERLGTRLQRLEAQVAGLAETTQTLQQRPAAATATVLQDARQALEARIAQFEQALSDRA
                     TADDLQALRAEVEQIKVRQTAARAAVPVQPRAPGKPAAAKPEPPPPLPFRVVGAELRA
                     GQRSVSVAPGAGDFTPDQLQVLLPGDAVGPWRLLAIDGNAAVFQIDGQVRRLAIP"
     gene            1533..2258
                     /locus_tag="PKV30_08860"
     CDS             1533..2258
                     /locus_tag="PKV30_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HRL30358.1"
                     /translation="MKPAILLAMLLLVSPQWTAWAQQPATAQPRNAQSQERPLVGRAL
                     DDRAARDWGLQPQEWARYQELMAGPLGIHSPNLDPLSALGIEARSDEERRRYAEMQVQ
                     AEARRVEKLLAYQRAYDEAWQRLNPGMQRVNLPDDRSNAAAMRGSGRTAVFIKDGCVA
                     CGQLVQRLQTSGAEFDLYMVGSRQDDARIRDWAKRAQIDPARVRSGSITLNHDGGRWL
                     SLGLPGDLPAVVREANGQWQRQP"
     gene            2240..2845
                     /locus_tag="PKV30_08865"
     CDS             2240..2845
                     /locus_tag="PKV30_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL30359.1"
                     /translation="MAAPAVAATSVSQFLRALVLAAALYVCATHAQEVPPPAYQLAAQ
                     RAGIPSTVLYAVALQESGVRRNGRIVPWPWSLNVAGQSHRYATRADACAGLQQAIRAT
                     PHTRIDAGLGQINLGYHQQRYSTACDLLDPYRNLAIAAEILHEQHTPGEDWLLAIGRY
                     HRPAGGEPAARYRRSVSRHLARVQGARPTTAASTARQETSP"
     gene            2842..3390
                     /locus_tag="PKV30_08870"
     CDS             2842..3390
                     /locus_tag="PKV30_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HRL30360.1"
                     /translation="MTKSHLANLTFKSLLMLLTALPLASLAGEPLIVVEDRGGVSALP
                     YYEALNLQPRGNGAARSPIPMPQVPATPADEAAMLPVRSAKLTPGTVARRVIEAPGLR
                     PFVVVGDDKASQDWLQRHAASLRERGAVGLVVNVETVQGLARLRALALGVPLAPVSGD
                     DLAERLGLRHYPALVTATGIEQ"
     gene            3395..>5106
                     /gene="traD"
                     /locus_tag="PKV30_08875"
     CDS             3395..>5106
                     /gene="traD"
                     /locus_tag="PKV30_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HRL30361.1"
                     /translation="MSGKQPVEVLLRPAVELYTVAACAGAAFLSLVAPWSLALSPAMG
                     IGSALAFSAYGAIRYRDARVILRYRRNIRRLPRYVMTSRDVPVSQQRLFIGRGFLWEQ
                     KHTHRLMQTYRPEFRRYVEPTPAYRLARRLEERLEFAPFPLSRLPRLTGWDVPFNPVR
                     PLPPVGGLPRLHGIEPEEVDVSLPLGERVGHSLVLGTTRVGKTRLAELFVTQDIRRKN
                     AAGEHEVVIVIDPKGDADLLKRMYVETKRAGREGEFYIFHLGWPEISARYNAVGRFGR
                     ISEVATRIAGQLSGEGNSAAFREFAWRFVNIIARALVELGQRPDYMLIQRHVINIDAL
                     FIEYAQHYFAKTEPKAWEVIVQIEAKLNEKNIPRNMIGREKRVVALEQYLSQARNYDP
                     VLDGLRSAVRYDKTYFDKIVASLLPLLEKLTSGKIAQLLAPNYSDLADPRPIFDWMQI
                     IRKRAVVYVGLDALSDAEVAAAVGNSMFSDLVSVAGHIYKHGIDDGLPDASAGARVPI
                     NVHADEFNELMGDEFIPLINKGGGAGLQVTAYTQTLSDIEARIGNRAKAGQVIGNFNN
                     LFMLR"
BASE COUNT          828 a         1819 c         1633 g          826 t
ORIGIN      
        1 ctgatggcca agaagatcat ggtctcgcag tccacctcgg gcgatgtacc agagtccggg
       61 ctcgatgtcc tgaaccagga tggcgattcc gtcgaggttg ccctcgcaag gcaacttgtg
      121 atggtttgat cctttccact tgcggccctt cggggccttt tttttggcag gagcgctgcc
      181 ggcaaatcgg acgctgtgcg attcgcattg tttgctgtcg cgtgtagacg tagcggcgat
      241 ggtgagggct cgatgtttca ggacaccgtg ctatgtgccc atcccatccc cggttccacc
      301 atcccgaacg ccgcctgctg tcggggtttc tcggcctgct cttatcggtc ctggccggtg
      361 gctgcgcgac gacgaccacg acgcagatgg cgcccgacgt cattgcgatt gaggaagcct
      421 cgcccggacc ccagcccgaa gccgcccagt tcattccggt cgtgcgctac ggtcgctaca
      481 ccctcgtcga gcttgcgccg atggctgcac agcgcgactt gctgctgcaa accgtcgatg
      541 tatctatgcc cgaagacgcc cgagcaacgg tcggcgatgg gctgcggcat gtgctcaagc
      601 gcagcggcta tcagctttgc gagacggcgc atgccgtgat cgagttgtac acgctgccgc
      661 tgccagcggc gcacctgcat ctgggcccca tgagcctgcg cgatgcgttg ctcaccctgg
      721 ccggcccggc ctgggaactg cacgcggatg accgtacgcg ccaagtctgc tttgagcaga
      781 tcggcgaacc cactggcggc gccccgattc ttgaaccgcc cgccaccgcc gccgcggcgc
      841 agccgttccc cctcgcggga ggccggccat gaacacttcg caatcttccc ggcgcccgac
      901 cgccgccgtg gtggtgcaga gcctgctctg gctttggctg atctgcctca gcgtcctcgt
      961 ggttctgggc taccagacga tgagcgacct ggctgaccag gagcggcttg gcacccgcct
     1021 gcaacgcctc gaagcgcagg tagccggtct ggccgaaacg acccagacct tgcaacagcg
     1081 gcccgcagcc gcgaccgcca cagtcctgca agacgcccgt caggccctgg aggcgcgcat
     1141 tgcccaattc gagcaggcgc taagcgaccg cgcaactgcc gacgaccttc aggcgctgcg
     1201 cgcggaggtc gagcagatca aggtgcgcca gacggcagcg cgtgccgccg tgccggtcca
     1261 gccgcgcgcg cctggcaagc ccgctgccgc gaaacccgag ccaccgccgc cgctgccctt
     1321 ccgcgtcgtc ggcgccgaac tgcgcgccgg ccagcgcagc gtgtccgtcg cgccgggcgc
     1381 cggggatttc acgcctgacc agctccaggt gctgctgcca ggcgatgcgg ttggcccatg
     1441 gcgcttgctg gcaatcgacg gcaacgccgc cgtattccag atcgacgggc aggtccgccg
     1501 cctggcgatt ccttgatgga ggccgactcg ccatgaagcc cgcgatcttg ctcgccatgc
     1561 tcctgctggt ctcaccgcag tggaccgcct gggcacagca gcccgcgacg gcccagcctc
     1621 gcaatgcgca gagccaggaa cgtcccttgg tcggtcgcgc cctggacgac cgcgcggcac
     1681 gcgactgggg actgcaaccg caggagtggg cgcgctacca ggaactcatg gcagggcctt
     1741 tgggcatcca ttcgcccaac ctggacccgc tttccgcgct gggcatcgag gcccgctccg
     1801 acgaagagag acgccgctac gcggagatgc aggtgcaggc cgaggcgcgc cgcgtggaga
     1861 agttactcgc ctaccagcgc gcctacgacg aagcctggca gcggctgaac cccggcatgc
     1921 agcgcgtgaa cctccctgat gacaggtcga atgctgccgc catgcgcggc agtggtcgca
     1981 ccgcggtttt catcaaggac ggctgcgtcg cctgcggtca actcgtgcag cgcctgcaga
     2041 cttcaggggc cgagttcgac ctgtacatgg tcggcagccg ccaggacgat gcgcgcatcc
     2101 gcgactgggc caagcgcgcg cagatcgacc cggcgcgcgt gcgcagcggc agcatcacgc
     2161 tcaaccacga cggcggccgc tggctgtcgc tgggcctgcc cggcgacttg ccagcggtcg
     2221 tgcgtgaggc gaacggccaa tggcagcgcc agccgtagcg gccacctccg tttctcagtt
     2281 cttgcgcgca ctggtgctcg ctgctgccct gtacgtctgc gccacccacg cccaggaggt
     2341 tccgccaccg gcctaccagc ttgccgccca gcgcgccggc atcccctcga cggtgcttta
     2401 cgccgtggcc ttgcaggaga gcggcgtccg gcgcaacggg cgcatcgtcc cgtggccttg
     2461 gtccctcaac gtcgcaggcc agtcgcaccg ttacgcgacc cgagccgacg cctgcgctgg
     2521 tttgcagcag gcaatacgag ccacgccgca cacacgcatc gacgcaggcc tggggcagat
     2581 caacctcggc taccaccagc agcgctacag caccgcttgc gacctgctcg acccgtaccg
     2641 caacctcgcc atcgccgccg agatcctgca cgaacaacac acccccggcg aggactggtt
     2701 gctggcgatc ggtcgatacc accgccccgc aggtggcgag cccgccgccc gctatcggcg
     2761 cagcgtgtcc cgccacctcg cccgcgtgca gggtgcgcgc ccaaccactg cggcgtccac
     2821 cgcgcgccag gagacctccc catgacgaaa tcccatctgg ccaatctcac gttcaagagc
     2881 ctgctcatgc tgctgaccgc cctgccactg gcctcgcttg ccggcgagcc gctgatcgtg
     2941 gtcgaggacc gtggcggcgt atcggcgctg ccgtactacg aagcactcaa cctccagccg
     3001 cggggcaacg gtgcagcgcg gtcacccatc ccgatgccgc aggttcccgc gacacccgcg
     3061 gatgaagccg cgatgctgcc ggtgcgcagc gccaagctca cacccggcac cgtcgcgcga
     3121 cgggtgatcg aggcgcctgg cctgcggcca ttcgtggtcg tcggcgacga caaggcttcc
     3181 caggactggt tgcagcgcca tgcagcctcg ttgcgcgagc gcggcgcggt cggcctggtg
     3241 gtcaacgtcg agaccgtaca gggcctagcg cggctgcgcg cactggcgct cggcgtaccc
     3301 ctcgcgcccg tgtccggcga cgacctggcc gaacgcctgg gcctgcggca ctacccggcg
     3361 ctggttacgg ccaccggcat cgagcaatga agccatgtcg gggaaacagc cggtcgaggt
     3421 tctgcttcgc ccagcggtgg agctatatac cgtcgcggcg tgtgcaggcg ccgcgtttct
     3481 gtccctggtg gccccgtggt cgctcgcgct gagcccggcc atgggcatcg gcagcgcgtt
     3541 ggcgttcagc gcctacggtg ccatccgcta tcgcgatgcc cgcgtgatcc tgcgctaccg
     3601 gcgcaacatc cgccgtctcc cccgctacgt gatgaccagc cgcgacgtgc cggtcagcca
     3661 gcagcggctc ttcatcggtc ggggctttct ctgggagcag aagcacaccc ataggctgat
     3721 gcagacgtac cggccagagt ttcgccgcta cgtcgagccg accccggcct accggctggc
     3781 gcggcgactg gaagagcggc tggagttcgc gccgtttccg ctatcgcgcc taccgaggct
     3841 cacaggatgg gatgtgcctt tcaacccggt acggccgctg ccgcccgtgg ggggcctgcc
     3901 gcggctacac ggcatcgagc cggaggaagt ggacgtcagt ctgccgctcg gcgagcgcgt
     3961 cgggcattcc ctggtgctgg gcacgacgcg cgtgggcaag acacggctcg ccgagttgtt
     4021 cgtcacgcag gacatccgcc gcaagaacgc cgccggcgag catgaggtgg tcatcgtcat
     4081 cgaccccaag ggcgatgcag acctcctgaa gcgcatgtac gtcgagacca agcgcgcggg
     4141 ccgcgagggc gagttctaca tcttccacct tggatggccg gagatttccg cgcgctacaa
     4201 cgccgtgggg cgcttcggtc gcatctcgga agtcgccacc cgcatcgcag ggcagctctc
     4261 cggcgaaggc aacagcgcgg cgttccgcga gttcgcctgg cgcttcgtga acatcatcgc
     4321 gcgggcattg gtggagctgg ggcagcgccc ggactacatg ctgatccagc ggcacgtcat
     4381 caacatcgac gcgctgttca tcgagtacgc ccagcactac ttcgccaaga ccgagcccaa
     4441 ggcctgggag gtgatcgtcc agatcgaggc caaactcaac gaaaagaaca tcccgcgcaa
     4501 catgatcggg cgcgagaagc gcgtggtggc gctggagcag tacctctccc aggcacgcaa
     4561 ctacgacccc gtgctcgacg gcctgcgctc ggcggtccgg tacgacaaga cctatttcga
     4621 caagatcgtc gcatcgctgc tgcctctcct ggagaagctc accagcggca agatcgcgca
     4681 gctcctggcc ccgaactact ccgacctggc cgacccgcgc ccgatcttcg actggatgca
     4741 gatcatccgc aaacgggccg tcgtctatgt gggcctggat gcgctctcgg acgccgaagt
     4801 cgccgctgcg gtcggcaatt ccatgttctc cgatctcgtg tcggtcgccg ggcacatcta
     4861 caagcacggg atcgatgatg gcttacccga tgcatcggcc ggcgcgcgcg tgccgatcaa
     4921 cgtccacgcc gatgaattca acgaactcat gggtgacgag ttcattccgc tcatcaacaa
     4981 gggcggcggc gccgggctcc aagtcaccgc ctacacgcag acgctctccg atatcgaagc
     5041 ccgcattggc aaccgcgcga aggccggtca ggtgatcggc aacttcaaca acctgttcat
     5101 gctgcg
//
LOCUS       DAOMVO010000170         5101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4564_length_5101_cov_14.500396, whole genome shotgun sequence.
ACCESSION   DAOMVO010000170 DAOMVO010000000
VERSION     DAOMVO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4564_length_5101_cov_14.500396"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKV30_08880"
     CDS             complement(<1..763)
                     /locus_tag="PKV30_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30362.1"
                     /translation="MNPNPQALRARHLLALAALSAAALLAGCAAPGSTPDRGSPAAVA
                     GSAAAPQILVPPTSVGTWVDLGHVLAPWLAGDAPVPVSGPAAPTRIAGLRREDGRWLA
                     IVIAQVAPSGGAPCPMPNSLHVVDTGTTVDGCLRMRRDADFDRWLEQQHSVLYQWLEG
                     RGWASRPRAWVGYRVPADAGRAIEAHALIAPTLIEPTTRTNTDFLAGGQPGLQWARAF
                     AGATRAASGGGVLNVPPFPFAPQVAPPAPPVVVAAL"
     gene            complement(800..1963)
                     /locus_tag="PKV30_08885"
     CDS             complement(800..1963)
                     /locus_tag="PKV30_08885"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRL30363.1"
                     /translation="MLMGVPAETATGETRVAVTPETAKKLIAQGHTVRVQSGAGLRAS
                     ATDEAYQAAGADITDAAGAFGADMVLKVRSPSAAEIAQMKPGTVLVGMLEPFNADGLQ
                     RLAAAHLTAFALEAAPRTTRAQSMDVLSSQANIAGYKAVIMAADRYQKFFPMLMTAAG
                     TVKAARVVILGVGVAGLQAIATAKRLGAVIEASDVRPSVKEQVESLGAKFIDVPYETA
                     EEKEAAEGVGGYARPMPQSWLDRQKAEVAKRVAQADVVISTALIPGRAAPVLITEDMV
                     KSMKPGSVIIDMAAGKGAPNQDGTVGGNCPLTEADKTVQKHGVTIVGETNLASMVGAD
                     ASSLYARNVLDFLKLVLPPAAKGEPAAEFRIDPEDDIVVATLMAQGGAVTRKA"
     gene            2109..2594
                     /locus_tag="PKV30_08890"
     CDS             2109..2594
                     /locus_tag="PKV30_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRL30364.1"
                     /translation="MNTPRWKPSVTVAAVIARDGRYLLVEEDTADGLRLNTPAGHLEP
                     GESPQDGAVREALEETGRVFTPEALLGVYLAASNDAQGAPTTWLRFAFCGSAGAAGPA
                     RTLDTGIVRTVWLTPAEIEASRPRHRSPLVWRCVQDHLRGQRFDLAAVVTDASAQHRI
                     A"
     gene            2700..3446
                     /locus_tag="PKV30_08895"
     CDS             2700..3446
                     /locus_tag="PKV30_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL30365.1"
                     /translation="MSTFALLAAAAFGAGVLNAIAGGGTFLTFPALVWTGVPPIAANA
                     TSALAVCPGYLGSALGFRQELAALPRALLIREVVLSALGGVAGALLLLVTPAKVFAGV
                     VPWLLLFATVLFALGPRLARWAGGTQGGGLARWRHAGLLAVAVYGGYFNGGLGILLMA
                     LYLLVGEQRLNTVNALKNLNSFVLSVVSVIAFAAGGAIVWRQALLMAVFATLGGFAGA
                     RLARRLPVGVVRTIVIVTGVVMSALFFMRS"
     gene            complement(3565..3780)
                     /locus_tag="PKV30_08900"
     CDS             complement(3565..3780)
                     /locus_tag="PKV30_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30366.1"
                     /translation="MASPTACAASAAFLPLASMGWASSTAAPASAAAFCAIGSIDLPK
                     ASSWYITATRPVATVVRWRMMRVVSSK"
     gene            3918..4247
                     /locus_tag="PKV30_08905"
     CDS             3918..4247
                     /locus_tag="PKV30_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30367.1"
                     /translation="MLSAWPWCVSTPYPWSASQTLAKAFSAAASKQKLPINYDHWLGY
                     INGRVLSEVVRAAGANPTPATLAAALEKLGKLDLGGYAPQHGPGRHHGSSFTEITILG
                     PKGRYIR"
     gene            complement(4276..>5101)
                     /locus_tag="PKV30_08910"
     CDS             complement(4276..>5101)
                     /locus_tag="PKV30_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30368.1"
                     /translation="MSRATPHTQAILPHLAKHNIALIAPSTGAMLLHEPVNPHVFNVR
                     SSYQAETEKAVEHLHTITLDRIAVVHVDDSFGADCLAGAMKGFERLKFKPVAVIKADR
                     AKPDYAAIVPQLQAANAQAVLWIGSSVAVSDGVKALRAAGSAAQVVTVSNNASGGFIK
                     QLGNAAHGVIVTQVFPNVRAMGYGINREAEALAKAKGNVTLSPQMMEGFAGAKVLVEA
                     LRRAGPKPTRERILTALGGMGHYDLGGLELGYGPGDRTGLNFVELSIIGSDGRFRR"
BASE COUNT          685 a         1797 c         1799 g          820 t
ORIGIN      
        1 ccagcgccgc caccaccacg ggcggcgccg gtggcgcgac ctgcggcgca aacgggaacg
       61 gcggcacgtt gagcacgccg ccaccgctgg ccgcgcgcgt ggcgccggca aaagcgcgcg
      121 cccactgcag gcccggctgg ccaccggcca gaaagtcggt gttggtgcgc gtggtcggct
      181 cgatcagcgt cggggcgatc agcgcatgcg cctcgatggc gcggccagcg tcggccggca
      241 cgcggtagcc gacccaggcg cgcgggcgcg aggcccagcc gcggccttcc agccactgat
      301 aaagcaccga atgctgctgc tccagccagc ggtcgaagtc ggcgtcgcgc cgcatgcgca
      361 ggcaaccgtc gaccgtggtg cctgtgtcga ccacgtgcag gctgttcggc atcgggcacg
      421 gcgcgccacc gctgggcgcc acctgggcga tgacgatggc cagccagcgg ccgtcctcgc
      481 gccgcagccc ggcaatgcgc gtgggcgccg ccgggccgct gacgggcacc ggcgcatcac
      541 cggccagcca gggcgccagc acatgaccca gatcgaccca ggtgccgacg ctggtgggcg
      601 gcaccagtat ctgcggcgcg gccgcgctgc cggcgaccgc tgccgggcta ccgcggtccg
      661 gcgtgctgcc gggcgcggcg cagccggcca gcagtgcggc ggccgacagc gcggccagcg
      721 ccagcagatg gcgagcgcga agagcttgtg ggttggggtt catggtcgta ctgcagatga
      781 ataaggggcc cgcaaggcgt caggcctttc gcgtgacggc gccaccttgc gccatcagcg
      841 tggcgacgac gatgtcgtcc tcgggatcga tgcggaattc ggcggccggc tcgcccttgg
      901 cggcgggcgg cagcaccagc ttcaagaagt cgagcacatt gcgcgcgtac aggctgctgg
      961 cgtcggcgcc gaccatcgag gccaggttgg tctcgccgac gatggtcacg ccgtgctttt
     1021 gcaccgtttt gtccgcttcg gtcagcgggc agttgccgcc caccgtgccg tcctggttgg
     1081 gtgcaccctt gccggcggcc atgtcgatga tgaccgagcc gggcttcatg cttttgacca
     1141 tgtcctcggt gatcagcacc ggcgcggcgc ggccgggaat cagcgcggtg gaaatcacca
     1201 cgtcggcctg cgccacgcgt ttggccactt cggctttttg ccgatcgagc cagctttgcg
     1261 gcatggggcg cgcgtaaccg cccacgcctt cggcggcttc tttttcctcg gccgtttcat
     1321 acggcacgtc gataaatttg gcgcccagcg attcgacctg ctccttcacg ctcggacgca
     1381 cgtcgctcgc ctcgatgacc gcgcccaggc gcttggcggt ggcaatcgcc tgcaggccgg
     1441 ccacgccgac gcccagaatc accacgcgcg ccgccttcac ggtgccggcg gcggtcatca
     1501 gcatggggaa gaacttctga tagcggtcgg ccgccatgat gacggccttg tagccggcga
     1561 tgttggcctg gctcgacagc acgtccatgc tttgcgcgcg cgtggtgcgc ggcgcggcct
     1621 cgagcgcgaa cgccgtcagg tgggccgccg ccagccgctg cagaccgtcg gcattgaacg
     1681 gctccagcat gccgaccagc accgtgcccg gcttcatctg cgcgatctct gcggccgagg
     1741 ggctgcgcac cttcagcacc atgtcggcgc cgaaggcgcc cgcagcatcg gtgatgtcgg
     1801 cgccggccgc ctgataggcc tcgtcggtgg cgctggcgcg caggccggcg ccggattgca
     1861 cacgcaccgt gtggccctgc gcgatgagct tcttggccgt ctcgggggtc acggcgacgc
     1921 gggtttcacc agtcgccgtt tcggcgggca cgcccatcag catggcatct ctcctcggtt
     1981 cacgatgggg cgcggtcaaa acagccggcc cgacttgttg gatggcgagc ttaacaaaag
     2041 ccctcggggc cattgtgcgg catttttgca cgccttcgtg gcgcgcgggc gacgggataa
     2101 ttcgcgccat gaacaccccg cgatggaaac ccagcgtgac cgtggccgcc gtgattgcac
     2161 gggacggccg ctacctgctg gtggaagaag acaccgccga cggcctgcgc ctgaacacac
     2221 ccgccggcca cctggagccc ggtgaatcgc ctcaggacgg cgccgtgcgc gaggcgctgg
     2281 aagagactgg ccgcgttttc acgcccgaag ctctgctggg cgtgtacctg gcggcgtcca
     2341 acgacgctca gggcgcgccg accacctggc tgcgctttgc cttttgcggc agcgccggcg
     2401 cggccggccc cgcccggacg ctggacaccg gcatcgtgcg caccgtgtgg ctgacgccgg
     2461 ccgagatcga agccagccgg ccgcgccacc gcagcccgct ggtgtggcgc tgcgtgcagg
     2521 atcacctgcg cggccagcgc tttgacctgg cggcggtggt gacggacgcg agtgcccagc
     2581 accgcattgc ctgaccggcg ttgccagatg gtttgaagcc ttttcggcct ccagcgcttg
     2641 cctgacctgc gcaagcagct ctgtttttca tagtagacca cttcaacgtc ttggacccga
     2701 tcagcacctt cgccttgctt gcggccgccg cctttggcgc cggcgtgctg aacgccattg
     2761 cgggtggcgg cacctttctc acctttccgg cgctggtgtg gaccggcgtg ccgccgattg
     2821 ccgccaacgc caccagcgcg ctcgcggtct gccccggcta cctgggcagc gcgctcggct
     2881 ttcggcagga actggcggcg ctgccgcgcg cgctgctgat ccgcgaagtg gtcttgagcg
     2941 ccctgggcgg cgtggcgggc gccctgctgc tgctggtgac gccggccaag gtctttgccg
     3001 gcgtcgtgcc ctggttgctg ctgtttgcca ccgtgctgtt cgcgctgggc ccgcggctgg
     3061 cgcgctgggc cggcggcacg cagggcggcg gtctggcgcg ctggcgccac gccgggctgc
     3121 tggcggtggc ggtgtacggc ggctacttca acggcggcct gggcattttg ctgatggcgc
     3181 tgtacctgct ggtgggcgag cagcggctga acaccgtcaa cgcgctgaag aacctcaact
     3241 ccttcgtgct gtcggtggtc tcggtgatcg cttttgccgc tggcggcgcc attgtgtggc
     3301 gccaagcgct gctgatggcc gtgttcgcca ccctgggcgg ctttgccggc gcgcggctgg
     3361 cgcggcgcct gccggtgggc gtggtgcgca ccatcgtcat cgtcaccggc gtggtgatga
     3421 gcgctttatt cttcatgcgc agctgaggcg ggcgtctcgc gcggcccacc tgacgccggc
     3481 gggcgtgccg ctgatcgcct cgtacacccg cacgcccgcg ctgcgcaccg acgagtaccc
     3541 gctgtacttc accacccagg ccaactactt cgacgagacg acccgcatca tccgccacct
     3601 gaccactgtg gcgaccggcc gcgtggccgt gatgtaccag gacgacgcct tcggcaagtc
     3661 gatgctgccg atggcgcaaa aggccgccgc cgacgccggc gccgccgtgc tgctggccca
     3721 gcccatggat gccagcggca agaacgccgc cgaagccgcg caggcggtgg gcgaagccaa
     3781 gccgcaatct gtcgtgctgc tggtcgccgg ccccggcgtg gtgccagcca tccgcgcgct
     3841 gcgccgcagc gtgtcggcgc cgatctacac ctattcgctg gccatcagcg cggcggcggt
     3901 cgaggcgctg ggcgctgatg ctgtcggcct ggccgtggtg cgtttccacg ccctacccgt
     3961 ggtccgcgtc gcagacgctg gccaaggcat tcagcgccgc ggccagcaag cagaagctcc
     4021 cgatcaacta cgaccactgg ctgggctaca tcaacggccg cgtgctgagc gaggtcgtgc
     4081 gcgccgccgg cgccaacccg acgccggcca ccctggcggc cgcgttggaa aagctgggca
     4141 agctggatct gggcggctac gcgccgcagc acggccccgg ccgccaccac ggcagcagct
     4201 tcaccgagat caccatcctg gggcccaagg ggcgctacat ccgctgagcc tggccgcgct
     4261 gcggcgcggc gcgcttcagc gccggaaccg accgtcgctg ccgatgatcg acagctcgac
     4321 gaaattcagg ccggtgcggt cgcccggccc atagccgagc tccaggccgc ccaggtcgta
     4381 gtggcccatg ccgcccagcg cggtcaggat gcgctcgcgc gtcggcttgg ggccggcgcg
     4441 gcgcagcgcc tcgaccaata ccttggcgcc ggcaaagccc tccatcatct gcggcgacag
     4501 cgtgacgttg cccttggcct tggccagcgc ctcagcctcg cggttgatgc cgtagcccat
     4561 ggcacgcacg ttggggaaca cctgcgtcac gatcacgccg tgcgccgcgt tgcccagttg
     4621 cttgatgaag ccgcccgagg cgttgttgga caccgtgacc acctgcgccg ccgagcccgc
     4681 cgcgcgcagc gccttgacgc cgtcgctgac ggccacgctg gagccgatcc agagcaccgc
     4741 ctgcgcgttg gcggcctgca gctgcggcac gatggcggcg tagtcgggtt tggcgcggtc
     4801 ggccttgatg acggcgaccg gcttgaactt caggcgctcg aagcctttca tggcgccggc
     4861 caggcaatcg gcgccgaagg aatcgtccac atgcaccacg gcgatgcggt ccagcgtgat
     4921 ggtgtgcagg tgctcgaccg ccttctcggt ctcggcctgg tagctggagc gcacgttgaa
     4981 cacgtgcggg ttgaccggct cgtgcaacag catggcgccg gtggacggcg cgatgagcgc
     5041 gatgttgtgc ttggccaggt gcggcaggat ggcctgggtg tgcggcgtgg cgcggctcat
     5101 g
//
LOCUS       DAOMVO010000171         5098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4571_length_5098_cov_14.092604, whole genome shotgun sequence.
ACCESSION   DAOMVO010000171 DAOMVO010000000
VERSION     DAOMVO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_5098_cov_14.092604"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKV30_08915"
     CDS             complement(<1..1007)
                     /locus_tag="PKV30_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRL30369.1"
                     /translation="MNAPQPPAPLSAQWFDGRSSRARPAQMRLVASPNGTRLQLRAQD
                     GAGETLDLAHKQVGWPERWSAGRAPPRVAVDLGAHGSLQIDDVAGWQAALAQAGHRSS
                     LAERMQTRWSVLLAVLLIAGAGVWAFYRWGTPWAATQITRHVPLAWELALTEQAMKQI
                     DADYVKPSKLPPARQAELRAGFDKLVALIGPDLQRYRGYRPPFKLEFRSGMGPNAFAL
                     PGGTIVMTDAMVEQARTIPNTGDTALLGVLAHEIGHVEHRHTTRMVVEQGVLNIGLGL
                     ALGDVSTLVSTGASLLTGLSYQRSHETESDCYAVAMMKKAGLPTAPMADLLLGVEHQM
                     E"
     gene            complement(1144..2379)
                     /locus_tag="PKV30_08920"
     CDS             complement(1144..2379)
                     /locus_tag="PKV30_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HRL30370.1"
                     /translation="MASTDPSLARGGIGAHPIEFTGSGGEYFRVWIVNVLLSIVTLGL
                     YTPWARRRTAQYFYSHTQVAGSPLEFVAETRRMVFGFLAFAALYIAYQLAAETGQQMA
                     AGLLLFGFAALAPFLWASAMRFRLGATRWRGIRLAFTASWKEVYIASWPVFAIAAIWT
                     AVFFVAAALAPDMPLGVAEEPGSVPKLPSFGGSALALGLLAVVLTLLCVIRLEYNYRR
                     LFIARARIGGQTGHWKPRYRDFVRVWLATVGFFILCALLVGVVMAVVMGGSMALLPRL
                     FGDVGGIAGMLLLMVLALLGALLLVVLASLPARAYREARMFQLVWNNVGVSTIARSKT
                     SLRTAAYVWLRVKNMLLTLITLGFYRPFAVASEYAAKVDSVTLYLKGGTDQLVGELVK
                     QQGAFGDAAADALGLDLIG"
     gene            complement(2577..4229)
                     /locus_tag="PKV30_08925"
     CDS             complement(2577..4229)
                     /locus_tag="PKV30_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL30371.1"
                     /translation="MPRPSQLLHPQRDPAPLRPAATVLLLRDSPQGIEVLMTRRSMTA
                     SFAPGAYVFPGGGIDSADGEAHAHATRRPTQSDEHLTQAIAAIRESFEELGVLLARHA
                     DASPASAADIAALDRHAPFAPQCVARGLTLAAADVYVLAHWVTDRDLPRRFDVPFLVA
                     RMPEGQTPVADEAEQFEPVWVRPADALARHEAGQFFIIFPTIRTLQRLKDYATVDAVI
                     AACASEQPLWASCPRAGLLAGKEARYMEHEPPFGELELVTPDGQMLHALDWQTEQPVP
                     LLKNVMRLTAPNPGFMTGPGTNSYLVGDPHTGYIAIDPGPADAAHIERLWRAAGGQIK
                     AIVCTHSHADHSPGAKPLQALCNPPPPILGLPSQPTARANSEFTPDRSLQNGELLALA
                     GQALEGQINHTLRVIHTPGHAANHLCLLLEEDGLLFSGDHILNGSTTVIDPPDGDMTA
                     YLDSLDKLDAACAAHEARFILPAHGHVLGSARAVIAGLKKHRLAREAKVLAVMQADPD
                     GTMDDWVAKAYDDVPPRIWPVAKRSLLAHVERIQAMMAPGGA"
     gene            complement(4447..>5098)
                     /locus_tag="PKV30_08930"
     CDS             complement(4447..>5098)
                     /locus_tag="PKV30_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRL30372.1"
                     /translation="ATLAHYLKSIIAIVLNIVLILAILDIFGIRTTSFAALLAGAGLA
                     IGAAWSGMLGNFAAGVFMQVLRPYKVGDFIQAGGVTGTVKELGLFGTTIVTPDNVTTI
                     VGNGKVFGDNIQNYSALPVRRVDCVAKVANSVNPMEAIARLAPVIAAIPNVSQNPAPE
                     INILEFTPEGPKLAVRPYTHTDHYWQVYFDTNKAIVETFGAAGYPAPETPVAHRNV"
BASE COUNT          816 a         1664 c         1816 g          802 t
ORIGIN      
        1 tcttccatct ggtgttccac ccccagcagc agatcggcca tcggcgccgt gggcagaccg
       61 gcttttttca tcatggcgac ggcgtagcag tcggattcgg tctcgtggct gcgctggtac
      121 gacaggccgg tgagcagcga ggcgccggtg gagacgaggg tggacacatc gcccagcgcc
      181 agccccaggc cgatgttgag cacgccttgc tcgaccacca tgcgcgtggt gtggcggtgt
      241 tcgacgtggc cgatttcgtg cgccagcacg ccgagcagcg cggtgtcgcc ggtgttgggg
      301 atggtgcgcg cctgctcgac catggcgtcg gtcatgacga tggtgccgcc gggcagcgca
      361 aacgcgttgg ggcccatgcc ggagcggaat tcgagcttga acggcggccg gtagccgcga
      421 taacgctgca ggtcgggccc gatcagcgcc accagcttgt cgaagccggc gcgcagctcg
      481 gcctggcgcg cgggcggcag cttgctgggc ttgacgtagt cggcatcgat ttgcttcatc
      541 gcctgctcgg tcagcgccag ctcccacgcc agcggcacgt ggcgcgtgat ctgcgtggcg
      601 gcccagggcg taccccagcg gtaaaaggcc cacacgccgg cgccggcgat cagcagcacg
      661 gccagcagca cgctccagcg ggtctgcatg cgctcggcga gcgagctgcg gtggccggcc
      721 tgcgccagcg ccgcctgcca gccggccacg tcgtcaatct gcaggctgcc gtgcgcgccc
      781 agatcgaccg ccacgcgcgg cggcgcgcga ccagccgacc aacgctcggg ccagccgacc
      841 tgcttgtgcg ccagatcgag cgtttcgccc gcgccgtcct gcgcgcgcaa ctgcaggcgc
      901 gtgccattgg gcgatgccac cagccgcatt tgcgccggcc gtgcacgcga gctgcggccg
      961 tcgaaccact gggcggagag cggggcgggc ggctggggag cgttcatatg actgcgcctt
     1021 cggcgcatga ccgcgacctg cggcctgcga tgacggcgct gcgcgccatg actttggtca
     1081 tgcgcccgca gggcgcgtca tcgaaggcga agccgaggat catcaagcgc agcgagctca
     1141 tcgtcatccg atcaaatcga ggcccagcgc atcggcggcc gcgtcgccaa acgccccttg
     1201 ctgcttgacc agctcgccca ccagctggtc ggtgccgccc ttgaggtaga gcgtgaccga
     1261 gtcgaccttg gccgcgtatt cgctggcgac ggcaaacggg cggtaaaagc ccagcgtgat
     1321 cagcgtcagc agcatgttct tgacccgcag ccacacgtag gcggcggtgc gcaggctggt
     1381 cttgctgcgc gcgatggtgc tgacgccgac gttgttccac accagctgga acatgcgcgc
     1441 ctcgcggtag gcgcgcgccg gcaggctggc cagcaccacg agcagcagtg cgcccagcag
     1501 cgccagcacc atcagcagca gcatgccggc gatgccgccg acgtcgccga acagcctggg
     1561 cagcagcgcc atcgagccgc ccatgaccac cgccatcacc acgccgacca acagcgcgca
     1621 caagatgaag aagcccaccg tggccagcca cacgcgcaca aaatcgcggt agcgcggctt
     1681 ccagtgcccg gtctggccgc cgatgcgggc gcgcgcgatg aacaggcggc ggtagttgta
     1741 ttcaagccgg atcacgcaca gcagcgtcag caccacggcc agcaggccca gcgccagcgc
     1801 gctgccgcca aagctgggca gcttgggcac gctgccgggc tcttcggcca cgcccagcgg
     1861 catgtcgggc gccagcgcgg cggcaacaaa gaacactgcc gtccagatcg ccgcgatggc
     1921 aaacaccggc caactggcga tgtagacctc tttccagctt gcggtgaagg cgaggcgaat
     1981 gccgcgccag cgcgtggcgc ccaggcgaaa gcgcatggcg ctggcccaca gaaacggcgc
     2041 cagcgctgca aagccgaaca gcagcaggcc ggcggccatc tgttggcccg tttcagccgc
     2101 cagctggtag gcgatgtaca gcgcggcaaa ggccagaaag ccgaacacca tgcgccgcgt
     2161 ctcggccacg aattcaagcg ggctgccggc gacctgcgtg tggctgtaga aatactgcgc
     2221 cgtgcgccgc cgcgcccacg gcgtgtacaa gcccagcgtg acgatggaca gcagcacgtt
     2281 gacgatccag acgcggaaat actcgccgcc cgagccggtg aattcgatcg gatgcgctcc
     2341 tattccgccg cgcgccagtg atgggtcggt gcttgccatg aatgccctcg tcctcttagg
     2401 tgtaccttgg tgcccagtgt agcgatggcc atgaccgcgc tgccgcgcga tgacctcggc
     2461 tttgccttcg atgacggcgc tgcgcgccat gacgaatgct cagggaaccc cagcgatcaa
     2521 cgacttccgt tgttcatgcg ccttcgggcg cagtcatcga agcccgcagg gcgaggtcat
     2581 gcgccgccag gcgccatcat ggcctggatg cgctccacat gcgccagcag cgaacgcttc
     2641 gcaacgggcc agatgcgcgg tggcacatcg tcgtacgcct tggcgaccca gtcgtccatc
     2701 gtgccatcgg ggtccgcctg catcacggcc agcaccttgg cttcgcgcgc cagccggtgt
     2761 tttttcaggc cggcgatgac ggcgcgcgcg ctgcccagca cgtggccgtg ggcgggcagg
     2821 atgaagcgcg cttcgtgcgc ggcgcaggcg gcgtcgagct tgtcgagcga atcgagatag
     2881 gccgtcatgt cgccatcggg cgggtcgatg acggtggtgc tgccgttcag gatgtggtcg
     2941 ccgctgaaca gcaggccgtc ttcttcgagc agcaggcaca ggtggttggc ggcgtggccg
     3001 ggcgtgtgga tgacgcgcag ggtatggttg atttggccct ccagcgcttg tccagcaagc
     3061 gcgagcagct caccattttg tagcgaccga tcgggcgtga attcgctgtt ggcgcgcgcc
     3121 gtgggttgcg agggcaggcc caggatcggc ggcggcgggt tgcacagcgc ctgcagcggc
     3181 ttggcgccgg gcgagtggtc ggcgtgcgaa tgggtgcaga cgatggcctt gatttgcccg
     3241 cccgccgcgc gccacagccg ctcgatgtgc gccgcatcgg ccgggccggg gtcgatggcg
     3301 atgtagccgg tgtgcgggtc gcccaccagg tagctgttgg tgccgggccc ggtcatgaag
     3361 ccggggttgg gcgccgtcag gcgcatcacg tttttcagca gcggcaccgg ctgctcggtc
     3421 tgccagtcga gcgcgtgcag catctgaccg tcgggggtca ccagttccag ctcgccaaag
     3481 ggcggctcgt gttccatgta gcgcgcctct ttgccggcca gcagaccggc gcgcgggcaa
     3541 ctggcccaca acggctgctc gctggcgcag gcggcaatga cggcgtcgac cgtggcgtaa
     3601 tccttcagcc gctgcagcgt gcggatggtg gggaagatga tgaagaactg ccccgcctcg
     3661 tgccgcgcca gcgcgtcggc cgggcgcacc cacacgggct cgaactgctc ggcctcgtcg
     3721 gccacgggcg tctggccttc gggcatgcgg gcgacgagaa agggcacgtc gaagcggcgc
     3781 ggcaggtcgc ggtcggtgac ccaatgcgcc agcacgtaaa cgtccgcagc ggcgagcgtc
     3841 aggccgcgcg cgacgcattg gggcgcgaag ggcgcgtggc gatccagcgc ggcgatgtcg
     3901 gcggcgctgg ccggcgaagc gtcggcgtgg cgggccagca gcacgcccag ttcttcaaag
     3961 ctctcgcgga tggcggcgat ggcttgggtc aggtgctcat cgctctgcgt cgggcggcgc
     4021 gtggcgtgtg catgggcctc tccatcggcg gagtcgatgc cgccgccggg gaacacgtag
     4081 gcaccgggcg cgaagctggc cgtcatcgaa cggcgcgtca tcagcacctc gatgccttgc
     4141 gggctgtcgc gcagcagcag cacggtggcg gcggggcgca gcggcgccgg gtcgcgctgc
     4201 gggtgcagaa gttggctggg gcggggcatg ggccgattat gggatcggcc ggggcgcggc
     4261 gttgtcgctt ggaaaatact acaaaaaaag gagctgcttg cgcttgtcac gcaagcgctg
     4321 gaaggcgatt tgacattgaa attcgatggt gggagcggcc ttggctgcga tgttggcgcg
     4381 tcgcatgtgc tgtcgcgtca tcgcggccaa ggccgctccc acgaggggcg atgccccaga
     4441 tcgacatcag acgttccggt gcgccaccgg cgtctcaggt gccgggtaac cggccgcgcc
     4501 gaaagtctcg acgatggcct tgttggtgtc gaaataaacc tgccagtaat ggtcggtgtg
     4561 cgtatagggg cgcaccgcca gcttggggcc ttcgggcgtg aattcgagga tgttgatctc
     4621 gggtgcaggg ttttgcgaca cgttggggat ggcggcgatc actggcgcca ggcgcgcgat
     4681 ggcctccatc gggttcacgc tgttggccac cttggccacg caatccacgc ggcgcacggg
     4741 cagggcgctg tagttctgga tgttgtcgcc aaacaccttg ccgttgccga cgatggtggt
     4801 cacgttgtcg ggcgtgacga tggtggtgcc gaacaggccc agctccttca ccgtgccggt
     4861 cacgccgccg gcctggatga aatcacccac cttgtacggc cgcagcacct gcatgaacac
     4921 gcccgcggca aagttgccca gcatgccgct ccaggccgcg ccaatcgcca gtcccgcgcc
     4981 agccagcagg gcggcaaacg aggtggttcg gatgccgaaa atgtccagaa tcgccaggat
     5041 caggacgatg ttcagcacga tggcgatgat cgacttgagg taatgggcca gcgtggcg
//
LOCUS       DAOMVO010000172         5089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4578_length_5089_cov_17.270957, whole genome shotgun sequence.
ACCESSION   DAOMVO010000172 DAOMVO010000000
VERSION     DAOMVO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4578_length_5089_cov_17.270957"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..622
                     /locus_tag="PKV30_08935"
     CDS             194..622
                     /locus_tag="PKV30_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRL30373.1"
                     /translation="MLWIKALHIVFIASWFAGLFYLPRIFVNLAMVPPGSVAERERLL
                     LMARKLYRFMMPLAVLALALGLWLWLGYGIGRGPGNGWMHAKLAIVLLLIGYHLWCGR
                     VLAKFARNESTPGHVWFRWFNEAPVLMLLAAVILVVVKPF"
     gene            633..1835
                     /locus_tag="PKV30_08940"
     CDS             633..1835
                     /locus_tag="PKV30_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRL30374.1"
                     /translation="MNRYKSAALPLALLYAALVIYASLYPFTGWRDVGVAPWAYLRAP
                     LPQYWTRFDVGANLAGYAPLGFLLTLAMMRMRRGWPALTLATLCAAALSLVLEALQTY
                     LPLRVASNVDLALNAAGGLLGALVAFVLERLGAIDRWRRLRARWFVRNSRGALVLLAL
                     WPIGLLFPAPVAFGMGQVFERIEEGLAEMLDGTPFLGWLPVRDMELQPLLPGQEVMCV
                     ALGALVPCLLGYSVIRHRGRRALFALLALLAGVAVTALSAGLSYGPVNAWSWVHPPVQ
                     LGLWGALIGAALALSLPGRVIVPLLMVVLAAQVVLLNTAPQNPYFAATLQSWEQGRFI
                     HFHGLAQWVGWLWPYAAFIYLASRIGGSDGGQPAVPPVPAPIPAPPAETSAPTPHVPT
                     EAAPRIGP"
     gene            1832..2176
                     /locus_tag="PKV30_08945"
     CDS             1832..2176
                     /locus_tag="PKV30_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRL30375.1"
                     /translation="MSTTPPPSYYQRHIFFCLNKRDNGEPCCADHDAQAAFDHCKSRV
                     KKEGLAGPGKVRVNKAGCLDRCAGGPIAVVYPEAVWYTYVDKDDIDEIVESHLKNGQV
                     VERLLTPPNIGR"
     gene            2263..2901
                     /locus_tag="PKV30_08950"
     CDS             2263..2901
                     /locus_tag="PKV30_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL30376.1"
                     /translation="MNSQTQSLTLPGPAGAIEALLDLPEADLFASPRGTAVIAHPHPQ
                     FGGTMTNKVVQTIARACVQCGWRALRFNFRGVGASAGAYDEGRGELDDLLAVVAEQAP
                     LDQPLLLAGFSFGGFVTAGAIARLHTQRPIAGAVLVGTAASRFAVPPVPPELHPQTLV
                     VHGELDETVPLASVMDWARPQSLPVTVVPGGEHFFHGQLPLLKSLVVRHVRA"
     gene            2981..4138
                     /locus_tag="PKV30_08955"
     CDS             2981..4138
                     /locus_tag="PKV30_08955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRL30377.1"
                     /translation="MTFLTRFLGATLLAAASLASHAQAPQPPEIAAKSYLLVDVTAGQ
                     TLAAKDADSPVEPASLTKLMSAYLVFDALKAKKITLTQTLPVSQRAWKMPGSRMFIDP
                     KMQVPVEDLIKGMIVQSGNDATMALAEGVAGSAERFVQLMNDQAKALGMKGTSYKNVE
                     GLTEAGHTTTARDLALLSTRLMHDFPEYVHYYTIKKYRYPGTPATNDTNRNTLLFRDP
                     TVDGLKTGHTNAAGYCLVATAKRDFPNLQGRRLLSVVLGATSETARANESQKLLNWGY
                     TAYEGVKLFDAGKPVATPKVWKGKTPELKLGRTEPIVVAVPAGTAAQVKTEIARPEPL
                     IAPFLQGQAVATLKITQGGQPLAEVPLVALEPVEEAGFFGRAWDAMRLWIK"
     gene            4418..4795
                     /gene="rpsL"
                     /locus_tag="PKV30_08960"
     CDS             4418..4795
                     /gene="rpsL"
                     /locus_tag="PKV30_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRL30378.1"
                     /translation="MPTINQLVRQGRTVEKIKSKSPAMQNSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKKA"
     gene            4966..>5089
                     /locus_tag="PKV30_08965"
     CDS             4966..>5089
                     /locus_tag="PKV30_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRL30379.1"
                     /translation="MPRRREVPKREILPDPKYGNVELSKFMNVIMEGGKKAVAER"
BASE COUNT          793 a         1669 c         1712 g          915 t
ORIGIN      
        1 gccgggtggt gtgggagggg cagccgctac gatagcggct cccctatccc gatttctggg
       61 ctgcgcagca agcccagaga gggccgcgga gcaggccaca cgggagcgcc gcggaacggg
      121 cttggcccgg ccgccagcgc tgtccccctg cgggggaagg cgccgcaggc gactcagggg
      181 gagataattc ttcatgctct ggatcaaggc cctgcacatc gtcttcatcg ccagctggtt
      241 tgccggcctg ttctatctgc cgcgcatctt cgtcaacctg gccatggtgc cgcccggctc
      301 ggttgccgag cgcgagcgcc tgctgctgat ggcgcgcaag ctgtaccgtt tcatgatgcc
      361 gctggccgtg ctggcgctgg ctttgggcct gtggttgtgg ctgggctacg gcatcgggcg
      421 cggaccgggc aacggctgga tgcacgccaa gctggccatc gtgctgctgc tgatcggcta
      481 ccacctgtgg tgcgggcgcg ttttggccaa gttcgcgcgc aacgagtcga cgccagggca
      541 cgtctggttc cgctggttca acgaagcacc ggtgctgatg ctgctggccg ccgtgatcct
      601 ggtcgtcgtc aagccgtttt gacgcgccgg gcatgaaccg ctacaaatcc gccgcgctgc
      661 cgctggcgct gctgtacgcg gcgctggtga tctacgcgtc gctttacccc ttcaccggct
      721 ggcgcgacgt gggcgtggcg ccgtgggcct acctgcgcgc gccgctgccg cagtactgga
      781 cgcgttttga tgtcggcgcc aacctggccg gttatgcgcc gcttggcttt ttgctgacgc
      841 tggccatgat gcgcatgcgg cgcggctggc cggcgctgac gctggccacg ctgtgcgcgg
      901 cggcgctgtc gctggtgctg gaggcgctgc aaacctattt gccgctgcgc gtggcctcca
      961 acgtcgatct ggcgctgaac gcggccggcg ggctgctggg cgcgctggtg gctttcgtgc
     1021 tcgagcggct gggcgccatc gaccgctggc gccgcctgcg cgcgcgctgg ttcgtgcgca
     1081 attcgcgcgg ggcgctggtg ttgctggcgc tgtggccgat cggtttgttg tttcccgccc
     1141 cggtcgcctt cggtatgggg caggtgttcg agcgcatcga agagggcttg gccgagatgt
     1201 tggacggcac gccctttctc ggctggctgc ccgtgcgcga catggaattg caaccgctgc
     1261 tgcccggcca ggaggtgatg tgcgtcgcgc tcggcgcgct ggtgccttgc ctgctgggct
     1321 acagcgtgat ccgccaccgc gggcggcgcg cgctgtttgc gctgctggcg ctgctggccg
     1381 gcgtggcggt gacggcgctg tcggccgggc tgtcgtacgg gccggtcaat gcctggtcgt
     1441 gggtgcatcc gccggtgcag ctggggctgt ggggcgcgct gatcggcgct gcgctggcgc
     1501 tgtccttgcc cgggcgcgtg atcgtgccgc tgctgatggt ggtgctggcg gcgcaggtgg
     1561 tgctgctgaa cacggcgccg caaaacccct actttgcggc cacgctgcaa agctgggagc
     1621 aggggcgctt catccacttt cacggcctcg cgcagtgggt cggctggctg tggccatacg
     1681 cggcgttcat ctacttggcc agccgcatcg gcggctcgga cggtggccag ccggcggtgc
     1741 cgccagtgcc ggcgccgata cccgcgccgc cggccgagac ttcggcgccc acaccgcacg
     1801 tgcccacaga ggccgcacct agaatcggcc catgagcacc acgccgccgc cttcgtacta
     1861 ccagcgccac atcttcttct gcctgaacaa gcgcgacaac ggcgagccgt gctgcgccga
     1921 ccacgacgcg caggccgcgt tcgaccattg caagtcgcgc gtcaagaaag aaggcctggc
     1981 cggccccggc aaggtgcgcg tgaacaaggc cggctgcctg gaccgctgcg ccggcgggcc
     2041 gattgccgtg gtctatccag aagccgtctg gtacacctac gtcgacaaag acgacatcga
     2101 cgagatcgtc gaatcgcacc tgaagaacgg ccaggtcgtc gagcgcctgc tgacgccgcc
     2161 gaacatcggc cgttgagcgc ttttctgatc gtctggttac aagctttttc agcctctggc
     2221 gcacgccgga cgtgcgcgag cagctacaat ttgaatagct gaatgaattc ccagacccaa
     2281 tccctgacgc tgccaggccc ggccggcgcc atcgaggcgc tgctcgatct gcccgaggcc
     2341 gacctgttcg cttcgccgcg cggcaccgcc gtcatcgccc acccgcaccc gcagtttggc
     2401 ggcaccatga ccaacaaggt ggtgcaaacc atcgcccgcg cctgcgtgca gtgcggctgg
     2461 cgcgcgctgc gcttcaactt tcgcggcgtc ggcgccagcg ccggtgctta cgacgaaggg
     2521 cgcggcgagc tggacgatct gctggccgtg gtggccgagc aggcgccgct tgaccagccg
     2581 ctgctgctgg ctggcttttc gtttggcggc ttcgtcaccg ccggcgccat cgcgcgcctg
     2641 cacacgcagc ggccgattgc cggcgccgtg ctggtgggca ccgccgcctc gcgctttgcc
     2701 gtgccgccag tgccgcccga gctgcacccg cagacgctgg tggtgcatgg cgaactggac
     2761 gagaccgtgc cgctggcttc agtgatggac tgggcgcgcc cgcagtcgct gccggtcacc
     2821 gtggtgccgg gcggcgagca tttctttcat gggcagttgc ccctgctcaa gtcgctggtg
     2881 gtgcgccatg tgcgcgcctg atcgttcgga ttccaagcct tttaatgctc tagcgcttgt
     2941 ctgacaagcg cgagcagctc tttatttgat agtaaatacc atgaccttct tgacccgctt
     3001 cctgggcgcc acgctgctgg ccgccgcctc gctggccagc cacgcgcagg cgccgcagcc
     3061 gcccgaaatc gccgccaagt cctacctgct ggtcgacgtc accgccggcc agacgctggc
     3121 cgccaaggac gccgattcgc ccgtcgagcc ggcttcgctc accaagctga tgagcgccta
     3181 cctggtgttc gacgcgctca aggccaagaa gatcacgctg acgcagaccc tgccggtgag
     3241 ccagcgcgcc tggaagatgc ccggctcgcg catgttcatc gaccccaaga tgcaggtgcc
     3301 ggtggaggat ttgatcaagg gcatgatcgt gcagtccggc aacgacgcca ccatggcgct
     3361 ggccgaaggc gtggccggct cggccgagcg cttcgttcag ttgatgaacg atcaggccaa
     3421 ggcgctgggc atgaaaggca ccagctacaa gaacgtcgaa ggcctgaccg aagccggcca
     3481 caccaccacg gcgcgcgacc tggcgctgct ttcgacgcgg ctgatgcacg actttcctga
     3541 gtacgtgcat tactacacca tcaagaagta ccgctacccc ggcacgccgg cgaccaacga
     3601 caccaaccgc aacacgctgc tgtttcgcga cccgaccgtc gatggcctga agaccggcca
     3661 caccaacgcc gccggctact gcctggtggc gaccgccaag cgcgacttcc ccaacctgca
     3721 agggcgccgg ctgctgtcgg tggtgctggg cgcgaccagc gagacggcgc gcgccaacga
     3781 atcgcaaaag ctgctcaact ggggctacac cgcttatgaa ggcgtcaagc tgttcgacgc
     3841 cggcaagccg gtggcgacgc ccaaggtgtg gaagggcaag acccccgagc tgaagctggg
     3901 ccgcaccgag cccatcgtgg tcgctgtgcc cgccggcacc gcggcgcagg tcaagaccga
     3961 aatcgcccga cccgagccgc tgatcgcgcc gttcctgcag ggtcaggccg tggcgactct
     4021 gaagatcacc caaggcggcc agccgctcgc cgaagtgccg ctggtggcgc tggagccggt
     4081 ggaggaagcc ggcttcttcg gccgcgcctg ggacgcgatg cgtttgtgga tcaaatagga
     4141 cgctggcgca cgtcggacaa gcgcaagcag cttcgttttt cgtagcggct tgcgagctga
     4201 tgtggacggc tgttgtcgcg tcgccgcacg gcttgagctg cttcgctggg cctgatgcat
     4261 tgccggccac ccgcccgcag gctataatcg caggcttttc ccgcaatttt gctgcgtgcg
     4321 cccatcttgg gtgacggcgt gcgctttcca aggcaatgcg gggtttgacg cgcacacggc
     4381 gcgtttttca cgtgttttca cgtttttagg gacatcaatg ccaaccatca atcaattggt
     4441 gcgtcagggg cggacggtcg agaagatcaa gtccaagagc cccgcgatgc aaaactcgcc
     4501 gcagcgtcgc ggcgtgtgca cccgtgtgta caccacgacg ccgaagaagc cgaactcggc
     4561 gcttcgtaaa gtcgccaagg tgcgcctgac caacggtttc gaggtcatct cttacatcgg
     4621 cggtgaaggc cacaacctgc aagagcacag cgtggtgctg gtgcgcggcg gtcgtgtgaa
     4681 agacttgccc ggcgtgcgct accacatcgt gcgcggctcg ctcgatttgc aaggcgtgaa
     4741 agaccgcaag cagtcgcgct ccaagtacgg cgccaagcgc cccaagaagg cctgatcaag
     4801 ggctgtcatt tttcggtgct tgaatcgccg ggcaggcggt tgcaagcgga gtgacctgcc
     4861 aattcaggtc gagtaagtgc aggctcttaa tgggtttgcc gggcgagcgg agtcttgata
     4921 acgactgctc cggccgccaa ctgaagcaat taaaggtgaa tagaaatgcc acgtcgtcgc
     4981 gaagtcccca aacgtgaaat cctgccggat cccaagtacg gcaatgtcga gctgtccaag
     5041 ttcatgaacg tgatcatgga aggcggcaag aaggcggttg ccgagcgca
//
LOCUS       DAOMVO010000173         5063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4606_length_5063_cov_14.426917, whole genome shotgun sequence.
ACCESSION   DAOMVO010000173 DAOMVO010000000
VERSION     DAOMVO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_5063_cov_14.426917"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKV30_08970"
     CDS             complement(<1..296)
                     /locus_tag="PKV30_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL30380.1"
                     /translation="MNQASNRTDKIVVVDDDARIRDLLRRYLAQEGFEVIVAEDAKAL
                     SRIMLRDTIDLIVLDLMMPGEDGLSVCRRLRAAGDKTPIIMLTAKGEDVDRIVGL"
     gene            480..779
                     /locus_tag="PKV30_08975"
     CDS             480..779
                     /locus_tag="PKV30_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30381.1"
                     /translation="MWLGVGVLALPLGAWAQASGESSEMGPGINAQAGGVDAMAARSP
                     LWQAVDAMHRQREGAAAQDRRLSAEERQQLREQIRRASIRLDGETQLRNTHLGQR"
     gene            1009..1647
                     /locus_tag="PKV30_08980"
     CDS             1009..1647
                     /locus_tag="PKV30_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859590.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRL30382.1"
                     /translation="MSIAPPQNVLQLSANGQVEVQQDLLTLSLTTSREGADAAGVQAE
                     LRKALDAALAQVKTSAQPGQMDVRTGDFNVHPRYNRDGKISGWQGRADLILEGRDFPR
                     ITQAAARATTMAIGQVNFGLSREQRAKVQAEAQAQAIERFKQRAAEIAKAFGFAGYTL
                     REVSVSGEEGGVAPRMYAMAKTASAMSADAPVSVEPGKTVVQVTVSGTVQAR"
     gene            1817..2758
                     /locus_tag="PKV30_08985"
     CDS             1817..2758
                     /locus_tag="PKV30_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL30383.1"
                     /translation="MTEPALHHVNCPDAEGGHRMAYWQWGDAAAEHVVVCAHGLSRQG
                     RDFDVLARALLARAGGQGQPLRVVCPDVVGRGHSDWLKEPMGYQFSTYVGDMLALLGA
                     LQGAGPIRSFDWVGTSMGGLIGLTLAGVPELALPALLRRLVLNDVGPVIEWAALQRIG
                     QYLGKSGTFPTVEAAAASMRAISAGFGPHTDDEWLALSRPMLRPTPDGAWRLHYDPAI
                     AVPYAALKPEASAEGEAALWQMYDRVTAETLVLRGADSDLLSAATADAMRIRGPRPRV
                     VSFAGVGHAPTLVAADQVAAVLDFLLPAPATPRDDAR"
     gene            2755..4968
                     /locus_tag="PKV30_08990"
     CDS             2755..4968
                     /locus_tag="PKV30_08990"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRL30384.1"
                     /translation="MKTLTRPAPTTREPLADVVTATGGGAAALGDMRARARAFAEPLL
                     AGETTSSGENVLAHADATADILAGIGGSDEIQAAVYLSYASAQLSRPDEVIGKAFGDG
                     FARLAIEASKLERVQQRSRVSRQDLSGDSEQAGQQTEFVRKMLLAFSRDLRVILLRLA
                     SRLQTLRWCASARRPPAPSLLRESHEVFAPLANRLGIWQVKWEMEDLIFRAQEPDAYR
                     LVASLLDEKRIEREAALEQKRSEIESALRKQGVDASVTGRPKHISSIVRKMRGKGLDF
                     DRVFDVRALRIMVPTVVDCYSALGWVHQHFTPVPEEFDDYIAKPKPNGYQSLHTVVRD
                     EQGKPWEIQIRTPDMHAHAEHGVAAHWAYKEAGTKGYAGVSAASDYDAKIAVLRQLLA
                     WERDLSGAAPHYGLFDDRIYVLTPDASIVELPAGATPVDFAYTVHTELGHRCRGARVD
                     GAMVPLQTALKNGQTVEVIAAKEGGPSRDWLNPELHFLASNRARAKVRAWFNAQHARD
                     NVARGREMVEKLLQREGRTALSLDHLASELGFKTPDALFETVGKDELSLRAIENVWRP
                     PEPVPDADEQVLRKARPETTSSRGGVLVVGLDSLLTQPARCCKPAPPDDIAGYVTRGR
                     GVSVHRRDCKTFRELQAREPERVIEVGWSTPATGRTPAYPVDVAVEALDRQGLLRDIS
                     DVFAREKMNVIGVQTQSVKGWAWMTFTVEVSDTQRLGHVLRQVQDVSGVRVARRR"
BASE COUNT          817 a         1657 c         1737 g          852 t
ORIGIN      
        1 aggccgacga tgcggtccac gtcctcgccc ttggcggtga gcatgatgat gggcgtcttg
       61 tcgcccgcgg cgcgcaggcg acggcagacc gacaaaccgt cttcgcccgg catcatgagg
      121 tcgagcacga tcaggtcgat ggtgtcgcgc agcatgatgc gcgagagcgc cttggcgtcc
      181 tcggccacga tgacttcgaa accctcttgc gccaggtaac ggcgcagcag gtcgcggatg
      241 cgcgcatcgt catccacgac gacgatcttg tcggttcggt tgcttgcctg attcatcaaa
      301 acccttcggt cctatttgta acagaggcat tgtggcctcg aagcatcaat gaacccgggt
      361 ttacccgcgc ggtttttcag gctgttacat ctgttgcccc agcttggtgc tctctgaagg
      421 acgctcacct caagctgtgc gatggtcggc ttgacatgga caggaataga ttgatatcga
      481 tgtggctggg tgtgggcgtg ctggcgctgc cgcttggcgc gtgggcgcag gccagcggcg
      541 aatcgtccga aatggggccg ggcatcaacg cgcaggccgg cggcgtggac gccatggctg
      601 cgcgcagccc gctgtggcag gccgtggatg cgatgcaccg ccaacgcgaa ggcgcggcgg
      661 cgcaggatcg gcgcctgagc gcagaggaac gccagcagtt gcgcgagcag atccgtcgtg
      721 catcgatacg gctggacggc gaaacgcagc tgcgcaacac gcacctcggc cagcgttgaa
      781 gcggcagcag ccttcgcggc ttgaaaggct tgcagcggtt tcgtttttgt ccgacagcgc
      841 ccgtgtggca cccgtgccat catgacgccc cgcgccgccc tggtgcacaa gaaggctgtt
      901 tttcctccta tgaatgcacg tgttttccgt cccgccgccg gcgcgctttg cgcactgatg
      961 ctgaccgccg ctgccgcgca cgcccaaccc atcgccacac ccgccgcgat gtcgattgcc
     1021 ccgccgcaaa acgtgttgca gttgtctgcc aatggccagg tcgaggtaca gcaggatctg
     1081 ctgacgctgt cgctcaccac ctcacgcgaa ggcgccgatg ccgccggcgt gcaggcggaa
     1141 ttgcgcaagg cgctcgatgc ggcgctggcg caggtcaaga ccagcgccca gccgggccag
     1201 atggacgtgc gcacgggcga cttcaacgtg cacccgcgct acaaccgcga cggcaagatc
     1261 agcggctggc aaggccgcgc cgatttgatt ctggaaggcc gtgactttcc gcgcatcacg
     1321 caggccgccg cacgcgcgac gacgatggcg atcggccagg tcaactttgg cctgagccgc
     1381 gaacagcgcg ccaaggtgca ggccgaggcg caggcgcagg ccatcgaacg cttcaagcag
     1441 cgcgcggccg agatcgccaa ggcgttcggc tttgccggct acaccttgcg cgaagtcagc
     1501 gtgagcggtg aagaaggtgg cgtggcgccg cgcatgtatg ccatggccaa gacggccagc
     1561 gccatgtcgg ccgatgcgcc ggtgtcggtg gagccgggca agaccgtggt gcaggtcact
     1621 gtatcgggca ccgtgcaggc gcgctgagcc gcgcatggcc tgattttggg tgattttcgg
     1681 ctctggcgct tgtatagcaa gcgcgagcag ctattttttt gatagttgcc tgtacgctgt
     1741 tgccagccac tgtcgggcgt ttcatttacc atggtccttc acggcgcacc ccgcgccgtg
     1801 aacctcgcca agcaccatga ctgaacctgc tctccatcac gtcaactgcc ccgacgcgga
     1861 gggtggtcac cgcatggcgt actggcagtg gggcgacgcg gccgctgaac atgtggtggt
     1921 ctgcgcgcac ggcctttcgc gccagggacg cgactttgac gtgctggcgc gtgcgttgct
     1981 ggcgcgcgcc ggtgggcagg gtcagcccct gcgcgtcgtc tgccccgatg tcgtgggtcg
     2041 tggtcacagc gactggctca aggagccgat gggctatcag ttttcgacct acgtcggcga
     2101 catgcttgcc ttgctgggcg cgttgcaagg cgccggaccg atccgcagct tcgactgggt
     2161 cggcaccagc atgggcggct tgatcggcct gacgctggcg ggcgtgcctg aattggcgtt
     2221 gcccgcgctg ctgcgccgtc tggtgctgaa cgatgtcggc ccggtcatcg aatgggcggc
     2281 gctgcagcgc atcggccagt acctgggcaa gtcgggcact ttccccacgg tcgaagcggc
     2341 ggcggccagc atgcgcgcaa tttcagccgg ctttggcccg cacaccgacg acgaatggtt
     2401 ggcgctgtcg cgccccatgc tgcgaccgac gccggacggt gcctggcgcc tgcactacga
     2461 cccggccatc gccgtgcctt acgcggcact caagccagaa gcgtcggccg agggcgaagc
     2521 cgccttgtgg cagatgtacg accgggtgac ggccgagacg ctggtgctgc gcggcgccga
     2581 ttccgatctg ctgtcggccg ccaccgccga cgccatgcgc atacgcggcc cgcgcccgcg
     2641 cgtggtctcg tttgccggcg tgggccatgc gcccacgctg gtggctgccg accaggtggc
     2701 cgcggtgctt gattttctgc tgcccgcgcc agccacgccg cgcgacgacg cgcgatgaag
     2761 accttgacgc gtcctgcgcc gaccacccgc gaaccgctgg ccgatgtcgt caccgccacc
     2821 ggcggcggcg cggcagcgtt gggcgacatg cgggcgcgcg cgcgcgcctt tgccgagccg
     2881 cttttggccg gcgaaaccac ctcctcgggc gaaaacgtgc tggcgcacgc cgacgccacg
     2941 gccgacatcc tggccggcat cggcggctcg gacgagattc aggcggcggt gtacctcagc
     3001 tacgccagcg cgcagctcag ccgccccgac gaggtcatcg gcaaggcctt tggcgacggc
     3061 tttgcgcggc tggccattga agcttcgaag ctggagcgcg tgcagcagcg ctcgcgcgtc
     3121 agccggcagg atttgtcggg cgattccgag caggccggtc agcagaccga gttcgtgcgc
     3181 aagatgctgc tggccttcag ccgcgatctg cgcgtgattc tgctgcgcct ggcctcgcgt
     3241 ttgcagacgc tgcgctggtg cgcttcggca cgccggccgc ccgcgcccag cttgctgcgc
     3301 gagtcgcacg aggtgtttgc gccgctggcc aaccggctag gcatctggca ggtcaaatgg
     3361 gagatggaag acctgatctt tcgcgcccag gagcctgacg cctaccgcct ggtggcctcg
     3421 ctgctggacg agaaacgcat cgagcgcgaa gccgcactgg agcaaaagcg ctcggaaatc
     3481 gagagcgcct tgcgcaagca gggcgtggac gccagcgtga cggggcgccc caagcacatc
     3541 agctccatcg tgcgcaagat gcgcggcaag gggctggatt tcgaccgcgt gttcgacgtc
     3601 cgcgccctgc gcatcatggt gcccacggtg gtcgactgct acagcgcgct gggctgggtg
     3661 caccagcact tcacgccggt gccggaagag ttcgacgatt acatcgccaa gcccaagccc
     3721 aacggctatc agtcgctgca caccgtggtg cgcgacgagc agggcaagcc gtgggaaatc
     3781 cagatccgca cgcccgacat gcacgcgcac gccgagcacg gcgtggccgc gcactgggcc
     3841 tacaaggagg ccggcaccaa gggttatgcc ggtgtcagcg cggccagcga ctacgacgcc
     3901 aagattgcgg tgttgcgcca gctgctggct tgggagcgcg atttgtctgg cgccgcgccg
     3961 cactatggtt tgttcgatga ccgcatctat gtgctgacgc cggatgcatc catcgtcgaa
     4021 ttgccggcgg gcgccacgcc ggtcgatttt gcgtacacgg tccacaccga gctgggccac
     4081 cgttgccgcg gcgcccgtgt tgacggcgcc atggtgccgc tgcaaaccgc gctgaaaaac
     4141 ggccagacgg tggaggtcat cgccgccaag gagggcggcc cgtcgcgcga ctggctgaat
     4201 cccgagctgc acttcctcgc ctccaaccgc gcgcgcgcca aggtgcgcgc gtggttcaac
     4261 gcccagcacg cacgcgacaa cgtggcgcgc gggcgcgaga tggtcgagaa gctgctgcag
     4321 cgcgaaggcc gcacagcgct gtcgctggat catctggcca gcgagctggg cttcaagacg
     4381 cctgacgcat tgttcgagac cgtcggcaag gacgaattgt cgctgcgcgc catcgaaaac
     4441 gtctggcgcc cgcccgaacc ggtgccggat gccgacgagc aggtgctgcg caaggcgcgg
     4501 cctgaaacca ccagcagccg cggcggcgtg ctggtggtgg ggctggattc gctgctcacg
     4561 caacccgcgc gctgctgcaa gccggcgccg cccgacgaca tagcgggtta cgtgacgcgc
     4621 gggcgtgggg tgagcgtgca ccggcgcgat tgcaagacct ttcgcgaact gcaggcgcgc
     4681 gagccagagc gtgtgatcga agtcggctgg agcacaccgg ccaccggccg cacccccgcc
     4741 tatccggtcg acgtggcagt tgaagcactg gatcgccagg gccttctgcg cgacatctct
     4801 gacgtgtttg cgcgcgagaa gatgaacgtg atcggcgtgc agacgcaatc ggtcaagggc
     4861 tgggcgtgga tgacgtttac cgtcgaggtc agcgacacgc agcgcctggg acatgttctg
     4921 cgccaggtac aagacgtgtc cggcgtgcgc gtcgctcgtc gccgctgaac cgcgcgcgcc
     4981 gatctgctat actcagcagc ttcgaatctt tcaggcgcgt agctcagctg gttagagcac
     5041 caccttgaca tggtgggggt cgt
//
LOCUS       DAOMVO010000174         5050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4629_length_5050_cov_15.811011, whole genome shotgun sequence.
ACCESSION   DAOMVO010000174 DAOMVO010000000
VERSION     DAOMVO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_5050_cov_15.811011"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PKV30_08995"
     CDS             <1..171
                     /locus_tag="PKV30_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL30385.1"
                     /translation="RVDEDDAKGVADLRSKGMNVVDNVDKAKFVAALTTANAQFEKDF
                     GKANLDRIRNFK"
     gene            261..755
                     /locus_tag="PKV30_09000"
     CDS             261..755
                     /locus_tag="PKV30_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRL30386.1"
                     /translation="MKAVFLNLESWTTRIAMTLACLMLAIASGLAVFQVITRFVLEQP
                     AEWTEVLIRFSLIWMVFMGIPMAFRQGAMVSVDALYRAVPTHIKRVLDLVVALAALAL
                     MLIILWWGYDYAVRGRVQTMAGLESLSMFWAYVAMPVGALFSVIAIVANYLDPRRMEL
                     ETAQ"
     gene            904..2178
                     /locus_tag="PKV30_09005"
     CDS             904..2178
                     /locus_tag="PKV30_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRL30387.1"
                     /translation="MTSAMLITMVLGFALSVSVAVSIGLASIVGIQVTNANMLISAKE
                     IFNSINKFPLAAVPFFILAGNLMETGGISRRLVEFAKSIVGGVQGGLPMTCVLTCMIF
                     AAVSGSSVATTFAIGAILIPALIKHGYPTSYAAALQATSAELGVIIPPSIPMILYGVS
                     AEVSIGELFIAGFGPGILISGALMLFVWVYCKWQGWGKNDGDGRLGFFKATRQAAWAL
                     LMPVIILGGIYGGIFTPTEASAIAVVYALIVGMLIYREIGIKDLFPVLRRSVISSAVI
                     MFIIANAGLFAFLITRAGVPEAIGHWLEQVLQSPAMFLLGVNAALFIIGMFIETSAAI
                     IVLAPILAPVAAHFGIDPVHFGLIMVVNLALGMITPPFGVNLFAACTVARISLDKIIR
                     HLIPFVCVILACLLIITYVPALSLGLRDLVYR"
     regulatory      2277..2399
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2458..3645
                     /gene="gcvT"
                     /locus_tag="PKV30_09010"
     CDS             2458..3645
                     /gene="gcvT"
                     /locus_tag="PKV30_09010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRL30388.1"
                     /translation="MSAADAELLKTPLHDLHVELGARMVPFAGYSMPVQYPAGLMAEH
                     KHTREAAGLFDVSHMGQLKLVGADAAAAFESLMPVDVIGLGVDKQRYGLLLTDEGTII
                     DDLMFVNWGDPADTTGESRPRASGEREGPQLFAIVNGACKAGDIAHLQAKIGGRCQVL
                     PMSERALLALQGPKAVDALQRLVPGVEKLVFMTGGAFAWNGTDLYITRSGYTGEDGFE
                     ISIHGSQAEAFARALLAEPEVAPIGLGARNSLRLEAGLCLYGNDIDTTTTPVEAALNW
                     AMQKVRRTGGERAGGFPGADKVLAQLDGSQPPKRKRVALIAKERVPVREPAPLENMDG
                     QTIGQVTSGLISPTLNQPIAMGYVQPDYATVGTELFAMVRGKPVPMVVSSTPFVRTRY
                     HRG"
     gene            3713..4078
                     /gene="gcvH"
                     /locus_tag="PKV30_09015"
     CDS             3713..4078
                     /gene="gcvH"
                     /locus_tag="PKV30_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRL30389.1"
                     /translation="MSIKYTEDHEWIKVDGDIAIVGITEHAQDALGDVVFVELPEVGK
                     TLAQKDVAGVVESVKAAADVYMPVSGEVVEVNQALADEPSLANSDPMGAGWFFKVKLA
                     KADEVAALMDEAAYKAFAA"
     gene            4173..>5050
                     /locus_tag="PKV30_09020"
     CDS             4173..>5050
                     /locus_tag="PKV30_09020"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722214.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HRL30390.1"
                     /translation="MSHAPTLVSLENPGEFIARHIGITPEEERKMLAVIGETSRQALI
                     DSIVPRSIARTAPMNLPPAISEANALSELKQIAAKNKLMKSFIGQGYHGCHTPGVILR
                     NVLENPAWYTAYTPYQAEISQGRMEALVNFQTMVTDLTGMDIANASMLDEATAAAEAM
                     TLARRSVKANGSVIVVFGDAHPQTIEVMQTRAAPLGLTVKLANSAAEWDAAIAADDYF
                     AALIQYPASSGWLMDWSAEVEKIHAKGAAAIFAADLLALTLLKSPGDKGADIVVGSTQ
                     RFGMPMGAGGPHAAFMA"
BASE COUNT          875 a         1679 c         1600 g          896 t
ORIGIN      
        1 cgcgtggacg aggacgacgc caagggcgtg gccgacctgc gcagcaaggg catgaacgtg
       61 gtcgacaacg tcgacaaggc caagtttgtg gccgcgctga ccaccgccaa cgcccagttt
      121 gaaaaagact tcggcaaggc caacctggat cggatccgca acttcaagta acgcgggtta
      181 gccaatcgcc ctattagcca tagcgcgatg tgcccgcccg ccctgcgcgg cgggcatttt
      241 tgtttcagat cggggcgctc atgaaggccg tgtttctcaa ccttgaaagc tggaccacgc
      301 gcatcgcgat gacgctggcc tgcctgatgc tggccattgc atcgggcctg gccgtgttcc
      361 aggtcatcac ccgcttcgtg ctggaacaac cggccgaatg gaccgaagtg ctgatccgct
      421 tcagcctgat ctggatggtg ttcatgggca tcccgatggc ctttcgccag ggcgcgatgg
      481 tgagcgtgga cgcgctgtat cgcgccgtgc ccacgcacat caagcgcgtg cttgacttgg
      541 tggtggcatt ggccgcgctg gcgctgatgc tgatcatcct gtggtggggt tacgactacg
      601 ccgtgcgcgg ccgcgtgcag accatggccg ggctggaatc gctgtcgatg ttctgggcct
      661 atgtggcgat gccggtgggc gcgctgttct cggtcattgc catcgtggcc aactaccttg
      721 acccgcgccg catggaactg gagaccgccc agtgatgacg accacccctg agccggcctg
      781 cggccgtctt cccctcaagg ggacgcaccc agaggccggg gcaagcccgt tccgcgggcg
      841 ccctcgaagc ggcgcgtact ccgcgtgctt cgccatcagc ttggaatttc gggagcgcag
      901 cgcatgacgt ccgccatgct catcaccatg gtgctgggtt tcgcgctcag cgtctcggtc
      961 gccgtctcga tcggcctggc gtccatcgtc ggcattcagg tcaccaacgc caacatgctg
     1021 atttccgcca aggagatctt caactcgatc aacaaatttc cgctggccgc cgtgcccttt
     1081 ttcatcctgg ccggcaacct gatggaaacc ggcggcatct cgcgccggct ggtggaattc
     1141 gccaagagca tcgtcggcgg cgtgcagggc ggcctgccga tgacctgcgt gctgacctgc
     1201 atgatcttcg ccgcagtgtc gggctcgtcg gtggcgacca cgtttgccat tggcgccatc
     1261 ctgattccgg cgctgatcaa gcacggctac cccaccagct acgccgccgc gctacaggcc
     1321 accagtgccg agttgggtgt gatcattccg ccctcgatcc cgatgattct gtacggcgtg
     1381 tctgccgagg tgtcgattgg cgagctgttc atcgccggct tcggcccggg catcctgatc
     1441 agcggcgctc tgatgctgtt cgtgtgggtg tattgcaagt ggcagggctg gggcaagaac
     1501 gacggcgacg gtcgtctggg ctttttcaag gccacccggc aggccgcgtg ggcgctgctg
     1561 atgccggtca tcatcctggg cggcatctac ggcggcatct tcacgccgac cgaggcgtcg
     1621 gccatcgcgg tggtctatgc cctgatcgtc ggcatgctga tctaccgcga aatcggcatc
     1681 aaggacttgt tccccgtgct gcgccgatcg gtcatctcgt cggcggtaat catgttcatc
     1741 atcgccaacg ccgggctgtt cgccttcctc atcacgcgcg cgggcgtgcc cgaggcgatc
     1801 ggtcactggc tggagcaggt gttgcaaagc ccggccatgt tcctgctggg cgtgaacgcg
     1861 gcgctgttca tcatcggcat gttcatcgag accagcgccg ccatcatcgt gctggcgccc
     1921 atcctggcgc cggtagcggc gcactttggc atcgatccgg tgcatttcgg gttgatcatg
     1981 gtggtgaatc tggcgctggg catgatcacg ccgccctttg gcgtcaactt gttcgcggcc
     2041 tgcacggtgg cgcgcatctc gctcgacaag atcatccggc acctgatccc cttcgtgtgc
     2101 gtgatcctgg cctgccttct gatcatcacc tacgtgccgg cgctgtcgct cggcctgcgc
     2161 gatctggtgt atcgctgaag gcaagctaga atctttcggt caggagagag cgctgccatt
     2221 cacccacgaa tgcgcgccgc cgaaggccga acgctcaggc aaaaaggact ggcaaacttg
     2281 attctgctgg agagaggccg ccgccccgca cgggtcaggc ggcccaccga aggggcaagg
     2341 cccgtacgcc aaccggcggc gggttgaatc tctcaggtaa aggggacagc agggttccgc
     2401 gcccgccgcg tgtcggcggg caatgcaatt gaacaacgca ttcgaggagc cctgcgcatg
     2461 tccgctgccg acgctgaact cttgaagacc ccgctgcacg acctgcacgt cgaactcggc
     2521 gcgcgcatgg tgccgtttgc cggctattcc atgcccgtgc agtatcccgc cggcctgatg
     2581 gccgaacaca agcacacgcg cgaagcggct ggcctgttcg acgtttcgca catgggccag
     2641 ctcaagctgg tcggcgccga tgcggcggcg gcgttcgaga gcctgatgcc ggtggatgtg
     2701 atcggcctgg gtgttgacaa gcagcgctac ggcctgctgc tgaccgacga gggcaccatc
     2761 atcgacgacc tgatgttcgt caactggggc gaccctgccg acaccaccgg cgagagccgc
     2821 ccccgcgcat cgggggagcg tgaagggccg cagctgttcg ccatcgtcaa cggcgcctgc
     2881 aaggcgggcg acatcgcgca cctgcaggcg aagatcggcg gccgctgcca ggtgctaccc
     2941 atgtccgagc gcgccctgct cgccctgcaa ggccctaagg ccgtcgatgc attgcagcgc
     3001 ctggtgccgg gcgtcgagaa actggtcttc atgaccggcg gcgccttcgc ctggaacggc
     3061 accgacctgt acatcacgcg cagcggctac accggcgaag acggcttcga gatttccatc
     3121 cacggctcgc aggccgaagc ctttgcgcgc gcgctgctgg ctgagccgga agtcgcgccc
     3181 atcggccttg gcgcgcgcaa ctcgctgcgc ctggaagccg gcctgtgcct gtacggcaac
     3241 gacatcgaca ccaccaccac gccggtcgaa gcggcgttga actgggcgat gcaaaaggta
     3301 cgccgcaccg gtggtgagcg cgccggtggc ttccctggcg ccgacaaagt gctggcgcaa
     3361 ctggacggct cgcagccacc taagcgcaag cgcgtcgccc tcatcgccaa ggagcgcgtg
     3421 ccggtgcgcg aacccgcacc gctcgaaaac atggacggcc aaaccatcgg ccaagtcacc
     3481 agcggcctga tcagccccac gctgaaccag cccatcgcca tgggctacgt gcagcccgac
     3541 tacgccaccg tcggcaccga gctgtttgcc atggtgcgcg gcaagccggt gcccatggtc
     3601 gtcagcagca cgcccttcgt gcgcacgcgt tatcaccgcg gttgatttcc aaccaaatca
     3661 gcctcccgcg cccgtccctt ctgcgcaacc cactcttatt tttggagcaa tcatgagcat
     3721 caagtacacc gaagaccacg aatggatcaa ggttgacggc gacatcgcca tcgtcggtat
     3781 caccgagcac gcgcaggacg cgctgggcga cgtggtgttc gttgagttgc ccgaggtcgg
     3841 caagaccttg gcgcaaaaag acgtggccgg cgtggtcgaa agcgtgaagg ctgcggccga
     3901 cgtgtacatg cccgtcagcg gcgaagtcgt tgaagtcaac caggcgctgg ccgacgagcc
     3961 gagcctggcc aacagcgacc cgatgggcgc cggctggttc ttcaaggtca agctcgccaa
     4021 ggccgatgaa gtcgctgcgc tgatggacga ggcggcctac aaggccttcg ccgcctgacc
     4081 ctgcgctgcc acagcgccgc gcaaggcgca tcgaccggcc gtgaacggtt cggcggtgcg
     4141 ccctgcactt tgtttgcccg gagcaccttc gcatgtccca cgcccccacc ctcgtcagcc
     4201 tggagaaccc cggcgaattc atcgcccgcc acatcggcat cacgcctgaa gaagagcgga
     4261 agatgctcgc cgtcattggt gagacatcgc gccaggctct gatcgattcc atcgtgccgc
     4321 gcagcattgc gcgcacggca ccgatgaacc tgccgcccgc catcagcgag gccaatgcgc
     4381 tctcagaatt gaagcagatc gctgccaaaa acaagttgat gaaaagcttc atcggccagg
     4441 gctaccacgg ctgccacacg cccggcgtga tcctgcgcaa cgtgctggaa aacccggcct
     4501 ggtacaccgc ctacacgccg tatcaggccg agatcagcca gggccgcatg gaggcgctgg
     4561 tcaacttcca gaccatggtg accgacctga caggcatgga cattgcgaat gcctcaatgc
     4621 tggacgaggc caccgctgcg gccgaggcca tgacgctggc gcgccgttcg gtcaaggcca
     4681 acggcagcgt gatcgtcgtc tttggcgacg cgcacccgca gacgattgag gtaatgcaga
     4741 cgcgcgccgc gccgctgggc ctgaccgtaa agctggccaa ctccgccgcc gagtgggacg
     4801 cggccattgc cgccgacgat tacttcgccg ccctcattca ataccccgcc agcagcggct
     4861 ggttgatgga ctggagcgcc gaggtcgaga agattcacgc caagggcgca gccgccatct
     4921 ttgccgccga tctgctggcg ctgacgctgc tcaaaagccc cggcgacaag ggtgccgaca
     4981 tcgtcgtcgg cagcacgcag cgctttggca tgccgatggg cgcgggcggc ccgcacgcgg
     5041 ccttcatggc
//
LOCUS       DAOMVO010000175         5004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4692_length_5004_cov_15.793898, whole genome shotgun sequence.
ACCESSION   DAOMVO010000175 DAOMVO010000000
VERSION     DAOMVO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_5004_cov_15.793898"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /gene="gspM"
                     /locus_tag="PKV30_09025"
     CDS             <1..403
                     /gene="gspM"
                     /locus_tag="PKV30_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HRL30391.1"
                     /translation="LWLLALSPALRTLRQAEVQRAELAAEAQRMQQLQSEAAALKALP
                     KLGHDEAVRALEAAVKQRLGASAQMSMVGDRANVVLKQAPADALAQWLADARANARAN
                     PVEARLTRAGDNAPGAPVLWSGTLSLSLPGQ"
     gene            631..1443
                     /gene="gspN"
                     /locus_tag="PKV30_09030"
     CDS             631..1443
                     /gene="gspN"
                     /locus_tag="PKV30_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HRL30392.1"
                     /translation="MKTAAPRRVSTPPASRAPWGWALTGALLGLLLVGVLAAPARWLS
                     AGVARATGDMVQLAEPQGSLWNGSARLLLTGGAGSQDRAALPGRVQWRLRPALGGLNL
                     AMTADCCTPTGPLALRVSLLWGGARVALQDGQTQWPAALLAGLGTPFNTLQPQGELAL
                     ATQNLAAEWRAGRPSLTGGAELTLRHVSSRLSTLQPLGSYRVQLQGGDAPAFTLSTLE
                     GPLQLSGNGQWVGARLRFSGEATAAPEQQAQLANLLNLLGRRQGDRAIMSFG"
     gene            1577..3898
                     /gene="gspD"
                     /locus_tag="PKV30_09035"
     CDS             1577..3898
                     /gene="gspD"
                     /locus_tag="PKV30_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HRL30393.1"
                     /translation="MPHPAPSHLCALAFLLALGGALPTGALAQAADGTVAARGGNVTL
                     DFANAEIDAVARTMATITGRNVVVDPRVKGTMTLTSTAPVSPAEAMRLFGAQLRTQGF
                     ALVDNRGMVLVVPEAEAKLQSAAVSAGAVRGGGQIQTQIFRLTHENANNLVPILRPLI
                     SPNNTINVNPGTNALVITDYGDNLQRLGRIIAALDVSNATGVEIVRLRHAVASDLAPL
                     VARLIESGGGGTPAVAQGVPGQPGVPPAAMGVATGSGDGYRTTLVPEPRSNAIIVRAA
                     NPARLALAKTLIQQLDQAPQARPDGGSGNIHVVYLKNADATKLAVTLRAAMAALPGQA
                     QTTGAGGGSALPSAGSGLGAGATQPGTSGLGNSTSSSLASGPTINAAANNQPSTGGQI
                     QADPATNSLIISAPEPVYRELRAVIDKLDQRRAQVFVESLIAEVNMSKAADFGVQWQM
                     FGQSGNNVGVIGTNYGSGRATGTAPTSNILDIAAAMFGISRLPSTSTPSTTALAGLRP
                     NTGFNFAFGTRALGILANFMQSTGAGNVLSTPTLLTLDNEEAKIIVGKNVPMPTGSYT
                     NTGAGGATLNPFQTYERKDVGLTLRVRPQINENGTVKLVLYQEVSSVDAGTASNLSGP
                     TTSKRAIESSVLVDDGSIVVLGGLLEDAYGTSEDKVPVLGDVPIIGNLFKTQNRSLNK
                     TNLMVFLRPVIVRDAADTENYSLDRYDLMRSAQQGNQPQPSSVLQINQAPVMPPVVPA
                     SPDAWSRPAPPPPLINPAAGESNYYGGTTITKP"
     gene            3901..>5004
                     /locus_tag="PKV30_09040"
     CDS             3901..>5004
                     /locus_tag="PKV30_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRL30394.1"
                     /translation="MRYPLPYAFARTARLLLEDDGQALTLWHDGRPDAGHLSEVMRRF
                     GAAQQPLALQQSDPHTLAQRISTAYSQSESSAAAVVSEVESDADLSRIMQELPAVEDL
                     LESADDAPIIRMLNALLTQAARDGASDIHIEPYERLSSVRFRVDGALREVVRPNRALH
                     AALISRLKIMADLDIAEKRLPQDGRISLRLGTRAIDVRVSTIPSAHGERAVLRLLDKS
                     ESKLSLEAVGMLGDVLQKFERLIAQPHGIILVTGPTGSGKTTTLYASLARLDASRNNI
                     MTVEDPIEYDLPGVGQTQVNPRIELTFARALRAILRQDPDIVMIGEIRDIETAQIAIQ
                     ASLTGHLVLATLHTNDAASAVTRLIDMGVEPFLL"
BASE COUNT          823 a         1866 c         1643 g          672 t
ORIGIN      
        1 gctgtggctg ctggcgctgt cgcccgcgct gcgcacgctg cgccaggccg aggtgcagcg
       61 cgccgaactg gccgccgaag cgcagcgcat gcagcagttg caaagcgaag ccgcagcgct
      121 gaaggcgctg cccaagctcg gccacgacga agccgtgcgc gcactggaag cggcggtcaa
      181 gcagcgcctg ggcgccagcg cgcaaatgag catggtgggc gaccgcgcca acgtcgtgct
      241 caagcaggcg ccggcagacg cgctggcgca gtggctggcc gacgcccgcg ccaacgcgcg
      301 cgccaacccg gtcgaagcgc gcctgacgcg cgccggcgac aacgcgccgg gcgcgcccgt
      361 gctgtggagc ggcaccttgt cgctcagtct gcccgggcaa tgaggcgttg gttgaccgat
      421 gacttcgctt cgctcaatga ccggccctgg cgcgccatga caaatgacca agcgcacgtg
      481 ttgaacgacg ccgctggtca ttggtcatgc gcccgcaggg cgcgtcatcc cggctcagcc
      541 gggggtcatg cgccgaaggc gcgatcgtgg tggatcgcat gaagaccacc acgccccgcc
      601 gggtcatgcg ccgaaggcgc ggtcatccgc atgaagaccg ccgcaccccg ccgcgtcagc
      661 acgccgcccg ccagccgcgc cccgtggggc tgggcgctga ccggcgcgct gctcggcctg
      721 ctgctcgtcg gcgtgctggc ggcgccggcg cgctggctgt cggcgggcgt ggcgcgcgcc
      781 accggcgaca tggtgcaact ggctgagccg cagggcagcc tgtggaatgg ctcggcgcgg
      841 ctgctgctga cgggcggcgc cggcagccag gaccgcgctg cgctgcccgg gcgcgtgcag
      901 tggcggctgc ggccggcttt gggcggactg aatctggcca tgacggccga ctgctgcacg
      961 cccaccggtc cgctggcgct gcgcgtcagt ctgctctggg gcggcgcccg cgtggcgctg
     1021 caagacggcc agacgcagtg gccggccgca ttgctggccg ggctgggcac gcccttcaac
     1081 acgcttcagc cgcagggcga gctggcgctg gccacgcaaa acctggccgc cgaatggcgc
     1141 gccggccgcc ccagcctgac cggcggcgcc gagctgacgc tgcgccacgt gtcgtcgcgc
     1201 ctgtccacgc tgcagccgct cggctcgtac cgcgtgcagt tgcagggcgg cgacgcaccc
     1261 gccttcacgc tctccacgct ggaaggcccg ctgcaactgt ccggcaacgg ccagtgggtc
     1321 ggcgcgcggc tgcgcttttc gggcgaggcc acggccgcgc cggaacagca ggcgcaactg
     1381 gccaacctgc tcaacctgct cggccgccgc cagggcgacc gagccatcat gagtttcgga
     1441 tgaaccgccc gcgccaccgg cgggatgctt gaacggcttg tcacctatgg ttttttcgca
     1501 tcaaaacacc ggcccagcgc tggttggacg tgggtctttt gctacattac ggatagctgt
     1561 ctccgctcgt tccctcatgc cgcaccctgc cccttcccac ctttgcgccc tggcctttct
     1621 gctggcgctg ggcggcgcgc tgcccactgg cgcgctggcg caggccgccg acggcacggt
     1681 ggccgcgcgc ggcggcaacg tcacgctcga ctttgccaac gccgagatcg acgccgtggc
     1741 gcgcaccatg gccaccatca ccggccgcaa cgtggtggtc gacccgcgcg tgaagggcac
     1801 catgacgctg accagcaccg cgccggtcag cccggccgag gccatgcgcc tgtttggcgc
     1861 ccagttgcgc acgcaaggct ttgcgctggt cgacaaccgc ggcatggtgc tggtggtgcc
     1921 cgaggccgag gccaagctgc aatccgccgc cgtgtcggcc ggtgcggtgc gcggcggcgg
     1981 gcagatccag acgcagatct ttcgcctgac gcacgagaac gccaacaacc tggtgccgat
     2041 cctgcgcccg ctgatcagcc ccaacaacac catcaacgtc aaccccggca ccaacgcgct
     2101 ggtcatcacc gactacggcg acaacctgca gcgcctgggc cgcatcattg ccgcgctgga
     2161 tgtgtccaac gccaccggcg tcgagatcgt gcgcctgcgc cacgcggtgg cgtccgacct
     2221 ggcgccgctg gtggcgcgcc tgattgaatc cggtggcggc ggcacacccg cggtggcaca
     2281 gggcgtgccg ggccagcccg gcgtgccacc tgcggccatg ggcgtggcca cgggcagcgg
     2341 cgacggctac cgcaccacgc tggtgcccga gccgcgcagc aacgccatca tcgtgcgcgc
     2401 cgccaacccg gcgcggctgg cgctggccaa gacgctgatc cagcagctcg accaggcgcc
     2461 gcaggcgcgc cccgacggcg gcagcggcaa catccacgtc gtctacctga aaaacgccga
     2521 cgccaccaag ctggccgtca ccttgcgcgc ggcaatggcg gcgctgccgg ggcaggcgca
     2581 gaccaccggc gcgggcggtg gctcggcgct gccttccgcg ggctcgggcc tgggcgccgg
     2641 cgccacccag ccaggcacat cgggcctggg caacagcacc agcagcagcc tggccagcgg
     2701 gccgaccatc aacgccgccg ccaacaacca gccgtccacc ggcgggcaga ttcaggccga
     2761 cccggccacc aactcgctca tcatcagcgc gcccgagccg gtttaccgcg aactgcgcgc
     2821 cgtgatcgac aagctcgacc agcgccgtgc gcaggtgttc gtcgaaagcc tgattgccga
     2881 ggtcaacatg tccaaggcgg ccgacttcgg cgtgcagtgg cagatgttcg gccaaagcgg
     2941 caacaacgtc ggcgtgatcg gcaccaatta cggcagcggc cgcgccaccg gcacggcacc
     3001 caccagcaac atcctcgaca tcgcggcggc catgttcggc atcagccgct tgccgtccac
     3061 cagcactcct tcgaccacgg cactggcggg cctgcgcccc aacaccggtt tcaacttcgc
     3121 atttggcacg cgcgcgctcg gcattctggc caacttcatg cagagcaccg gcgctggcaa
     3181 cgtgctgtcc acccccacgc tgctgacgct ggacaacgag gaagccaaga tcatcgtcgg
     3241 caaaaacgtg ccgatgccga ccggcagtta caccaacacc ggagccggcg gcgccacgct
     3301 caatccgttc cagacctatg agcgcaagga cgtcggcctg acgctgcgcg tgcgcccgca
     3361 aatcaacgag aacggtacgg tgaagctggt gctgtaccag gaggtttcca gcgtcgatgc
     3421 gggcactgcg tcgaacctgt caggcccgac caccagcaag cgcgcgatcg aatccagcgt
     3481 gctggtggac gacggttcca tcgtggtgct gggcggcttg ctcgaagatg cctatggcac
     3541 ctcggaggac aaggttccag tactcggcga tgtccccatc attggcaacc tgttcaagac
     3601 ccagaaccga agcctgaaca agacgaacct gatggtgttc ctgcgccccg tgatcgtgcg
     3661 cgacgccgcg gacaccgaga actactcgct cgaccgctac gacctgatgc gctcggcaca
     3721 gcaaggcaac cagccccagc ccagcagcgt gctgcagatc aaccaggcgc cggtgatgcc
     3781 gcccgtggtg cccgccagcc ccgatgcttg gtcgcgcccg gcgccgccgc cgccgctgat
     3841 caaccccgcc gcgggcgaga gcaactacta cggcggcacc accatcacca agccgtagcc
     3901 atgcgctacc cgctgcccta cgcctttgcg cgcaccgcgc ggctgctgct ggaagacgat
     3961 ggccaggcgc tgacgctatg gcacgacggc cgccccgacg ccggccattt gagcgaagtc
     4021 atgcgccgct ttggcgccgc gcagcaaccg ctggcgctgc agcaatccga cccgcacacg
     4081 ctggcgcagc gcatcagcac cgcctattcg caaagcgaat ccagcgccgc cgccgtggtc
     4141 agcgaagtcg aatccgacgc cgacctgtcg cgcatcatgc aagagctgcc cgccgtcgaa
     4201 gacctgctgg aatcggccga cgacgcgccc atcatccgca tgctcaacgc cctgctcacg
     4261 caggccgcgc gcgacggcgc cagcgacatc cacatcgagc cgtacgagcg gctgtcgagc
     4321 gtgcgctttc gcgtcgacgg cgcgctgcgc gaagtggtgc ggcccaaccg cgcgctgcac
     4381 gcggcgctga tcagccgcct gaagatcatg gccgacctcg acatcgccga aaagcgcctg
     4441 ccgcaggacg gccgcatcag cctgcgcctg ggcacccgcg ccatcgacgt gcgcgtgtcc
     4501 accatcccca gcgcgcacgg cgagcgcgcg gtgctgcgcc tgctcgacaa gagtgagagc
     4561 aagctgagcc tggaagcagt gggcatgctg ggcgatgtgc tgcagaaatt cgagcgcctg
     4621 atcgcccagc cgcacggcat catcctcgtc accggcccca ccggcagcgg caagaccacc
     4681 acgctgtacg ccagcctggc gcggctggac gccagccgca acaacatcat gacggtggaa
     4741 gacccgatcg aatacgacct gcccggcgtc ggccagacgc aggtcaaccc ccgcatcgag
     4801 ctgaccttcg cccgcgccct gcgcgccatc ctgcggcaag accccgacat tgtgatgatc
     4861 ggcgagatcc gcgacatcga gacggcgcag atcgccatcc aggccagcct gaccggccac
     4921 ttggtgctgg ccaccctgca caccaacgac gccgccagcg ccgtcacccg gctcatcgac
     4981 atgggcgtgg agcctttcct gctc
//
LOCUS       DAOMVO010000176         4996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4705_length_4996_cov_15.231330, whole genome shotgun sequence.
ACCESSION   DAOMVO010000176 DAOMVO010000000
VERSION     DAOMVO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4705_length_4996_cov_15.231330"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..814
                     /locus_tag="PKV30_09045"
     CDS             119..814
                     /locus_tag="PKV30_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRL30395.1"
                     /translation="MIDVYSWPTPNGHKVHIMLEECGLPWRVHAVNIGKGEQFEADFL
                     AISPNNKIPAIVDPDGPDGAPISVFESGAILLYLAGKTGRFLPADVRGKYEALQWLMF
                     QMGGVGPMLGQAHHFRIYAPEQIDYAVNRYTNEAKRLYGVMDRQVARTGAHIAGREYS
                     VADIAIYPWLNSWNNQGIDWADHPALKDWFDRVGARPAVQRGIKVLAERRQPVRTDAE
                     KEVLFGAMQYQKR"
     gene            862..938
                     /locus_tag="PKV30_09050"
     tRNA            862..938
                     /locus_tag="PKV30_09050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Pro,seq:tgg)
     gene            1001..1077
                     /locus_tag="PKV30_09055"
     tRNA            1001..1077
                     /locus_tag="PKV30_09055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1035..1037,aa:Arg,seq:tct)
     gene            1237..1686
                     /locus_tag="PKV30_09060"
     CDS             1237..1686
                     /locus_tag="PKV30_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30396.1"
                     /translation="MDAGAAAVDTACAAPHRLSPSHLIFAFSKQESPTMRAIKEARKY
                     IERKPDDAHAKTLSRLVLALESEADFPISDLYKLDLARFKLALEILDEWRLDRHYASK
                     AKLFDVSLQMAVLKKPAPEAAAAPAAADAKRAKPDAKQDKPDGKAEK"
     gene            complement(1697..2812)
                     /gene="ribD"
                     /locus_tag="PKV30_09065"
     CDS             complement(1697..2812)
                     /gene="ribD"
                     /locus_tag="PKV30_09065"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRL30397.1"
                     /translation="MELTTPDACMRRALALAEQARLICSPNPAVGCVLVDAAGQLLGE
                     GHTQAVGQAHAEVMALRDAAARGHSTVGATAYVTLEPCAHHGRTGPCCDALVAAGVAK
                     VVASLQDPNPRVTGQGFARLRAAGVEVVIGPGAQATRDLNIGFFSRMVRGTPWVRLKA
                     AISLDGRTALPNGASQWITSAPAREDGHAWRARADAVLTGIGTVLQDDPLLNVRLPGA
                     TRQPMLAIVDSQLQTPPGARLWSAGPRGVVICTAVQDPARWQALQALGAQIVACPNAH
                     SKVDLAAMLKDLAQREVNEVHVEAGYKLNGSLMREGLVDEWLIYQAPKLLGDGPGVAA
                     LGPFTQIDQVPELSWRDVQRIGPDLRLRAVAAGRDEF"
     gene            complement(3005..4852)
                     /locus_tag="PKV30_09070"
     CDS             complement(3005..4852)
                     /locus_tag="PKV30_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30398.1"
                     /translation="MLGHFERLLPPYPDPEPELPPKGFLAFVWRATTGLRGYIGLMAL
                     LSAGTSIYDALLFALLGFVVDWLGQVAPGQLWAERGGVLLLLAGVLLASIALVALQTI
                     VKHQTLAINFPLRLRWNFHRLMLGQSMAFYADEFAGRITTKIMQTALAVRDMIFTTAD
                     VVIGMGVYMVTILLLAAGFDGWLMLPFLLWAVAYGLACWYFVPRLSKVSRAQADARSV
                     MTGRITDAYTNIATVKLFSHTRREAQFARAAMEDFRQTGYAQMRLVSIFETVNHALIV
                     LLILAACGTALWLWSIGQVGTGAVAAVTAMALRLSGMSHWVMWEMTSLFESVGTIQDG
                     INTLSRPRAVVDAPGAKPLVVTRGEVRFDDVTFSYRDGARPVIENLNLTIHPGEKVGL
                     IGRSGAGKSTLVNLLLRFHDVQKGRILIDDQDIAKVTQDSLRHAIGMVTQDTSLLHRA
                     MRDNILYGRPDATEAQMLTAAKRAQADEFIDQLTDLQGRRGYEAHVGERGVKLSGGQR
                     QRVAIARVMLKDAPVLLLDEATSALDSEVEAAIQESLQELMKGKTVIAIAHRLSTIAA
                     LDRLIVLDAGRVVEEGTHAQLLARGGIYAGLWSRQSGGFLGEQGEDGGV"
BASE COUNT          868 a         1672 c         1622 g          834 t
ORIGIN      
        1 tgtagtgatc cagcgcagcc ggccggcggg cccgctgcgc ttttttcatg gccggtgctg
       61 cacgcgcgcc gggcgggttg cacaatcgcg ctcgatcgca accacctgga gttcacccat
      121 gatcgacgtc tactcgtggc ccacgcccaa tggccacaaa gtccacatca tgctggaaga
      181 gtgcggcttg ccgtggcgcg tgcacgccgt caacatcggc aagggcgagc agttcgaggc
      241 cgacttcctc gccatcagcc ccaacaacaa gatcccggct atcgtcgatc ccgatgggcc
      301 tgacggcgca ccgatctcgg tctttgaatc gggcgccatc ctgctctacc tggccggcaa
      361 gaccgggcgt tttctgcccg ccgatgtgcg cggcaaatac gaagcgctgc aatggctgat
      421 gttccagatg ggcggcgtcg gccccatgct gggccaggcg caccactttc gcatctacgc
      481 gccggagcag atcgactacg ccgtcaaccg ctacaccaac gaggccaagc gcctgtacgg
      541 cgtgatggac cggcaggtgg cgcgcaccgg cgcccacatc gcgggccgcg agtacagcgt
      601 ggccgacatc gccatctacc cgtggctgaa ttcgtggaac aaccagggca tcgactgggc
      661 cgaccacccg gcgctgaaag actggttcga ccgcgtcggc gcgcgccccg cggtgcagcg
      721 cggcatcaag gtgctcgccg agcggcgcca gcccgtgcgc accgacgccg aaaaagaggt
      781 gctgttcggc gccatgcaat accagaagcg ttgagtgcgg ccgaatcgag gtcgcactca
      841 ggttagaatt cgccctttcg tcggagcgta gcgcagtctg gtagcgcatc tgctttggga
      901 gcagagggtc gcgagttcga atcccgccgc tccgaccatt tcttttcgtg ttttgcgaca
      961 caatccaaag gcccgttttg cgggcctttg tgctttttgt ctgcccgtag ctcaactgga
     1021 tagagcagct gccttctaag cagcaggtcg ggggttcgag tccctccggg caggccagcg
     1081 atctttcgga ctgcgcgccg tcaggggctg caatatgtca tatcgatgac agtcacatga
     1141 tgcgatagcg gcaatcgcca tcatcggcta gcatcgattt agcgttcggt tacgtccgcg
     1201 cggttcttcg ccgcgtcgcc cactggccga cgtgttttgg atgcaggggc ggctgccgtt
     1261 gatacggcct gcgccgcgcc gcatcgtttg tctccaagtc atctgatctt cgctttttcc
     1321 aaacaggaat ctccgaccat gcgtgccatc aaagaagccc gtaaatacat cgagcgaaag
     1381 cccgacgacg cccacgccaa gaccctctcg cgcctggtgc tggcactcga atccgaggct
     1441 gattttccga tctccgatct gtacaaactg gatctggcac gcttcaagct ggcgctggaa
     1501 atcctggacg aatggcgcct ggaccgccac tacgccagca aggcgaagct gttcgatgtg
     1561 tcgctgcaga tggcggtgct gaaaaagccc gcgcccgaag cggccgccgc accggctgca
     1621 gccgatgcca agcgcgccaa gccggacgcc aaacaggaca agccggacgg caaggccgag
     1681 aagtaagccc gcgcgctcag aattcgtcgc gccccgctgc cacggcgcgc aggcgcagat
     1741 cgggcccgat gcgctggacg tcgcgccagc tcagttcggg cacctgatcg atctgcgtga
     1801 aggggccgag tgccgccacg cccggcccat cgcccagcag cttgggcgcc tggtaaatca
     1861 gccactcgtc caccagcccc tcgcgcatca gcgagccgtt gagcttgtag ccggcttcaa
     1921 cgtgcacctc gttcacctcg cgctgcgcca gatccttcaa catggccgcc aggtcgacct
     1981 tgctatgcgc gttcgggcag gcgacgattt gtgcgcccag cgcttgcagg gcctgccagc
     2041 gcgccgggtc ttgcacggcg gtgcagatga cgacgccgcg cggcccggcg ctccacaagc
     2101 gggcgcccgg cggcgtttgc aactggctgt cgacaatcgc cagcatcggc tggcgcgtgg
     2161 caccgggcaa gcgcacgttg agcagcgggt catcttgcag cacggtgccg atgccggtca
     2221 gcaccgcgtc ggcgcgggcg cgccaggcgt ggccgtcttc gcgcgccggg gcgctggtga
     2281 tccactggct ggcgccgttc ggcagcgcgg tgcggccgtc cagcgagatc gctgccttca
     2341 gccgaaccca cggcgtgccg cgcaccatgc ggctgaaaaa gccgatgttg aggtcgcgcg
     2401 tcgcttgggc accggggccg atcacgacct cgaccccggc cgcgcgcaag cgtgcaaaac
     2461 cttgcccggt cacacgcggg ttcgggtctt gcagcgaggc gaccaccttg gccacgccgg
     2521 cggccaccag cgcgtcgcag cacgggccgg tgcggccgtg gtgcgcgcag ggctccagcg
     2581 tgacgtaggc ggtagcgccg acggtggaat ggccgcgcgc ggcggcatcg cgcagcgcca
     2641 tcacctcggc gtgggcctgg ccgacggctt gcgtgtgccc ctcgccgagc agttggcccg
     2701 ctgcgtccac cagcacgcag ccgaccgccg gattgggcga gcagatgaga cgcgcttgtt
     2761 cagccagcgc gagcgcgcgg cgcatgcagg catcgggggt ggtcaattcc atggcggcgg
     2821 ttgtggcggg cgaccggtcg actataggga atgcgcaggt gcggtgccgt gcgcgctgtt
     2881 gtgcgtcggg ctcgcggctt gccctcaatt tcagatcaaa tcggccgcca gcgcctgaat
     2941 agcaagcgcg agcagctatg ttttatatag taaacgatgg ctgagcaatg agcgcatcga
     3001 cggctcacac cccgccgtcc tcaccctgtt cacccagaaa gccgccgctc tggcgcgacc
     3061 acagcccggc gtagatgccg ccgcgcgcca gcagttgggc gtgcgtgcct tcttccacca
     3121 cgcggccggc gtcgagcacg atcaggcggt ccagcgcggc aatggtcgac aagcggtgcg
     3181 caatggcgat cacggtcttg cccttcatca gctcctgcag gctttcctgg atggccgctt
     3241 caacctctga atcgagcgcg ctggtcgctt cgtccagcag cagcaccggc gcgtccttca
     3301 gcatcacgcg ggcaatcgcc acgcgctggc gctggccgcc cgacagcttg acgccgcgct
     3361 cgcccacgtg ggcctcgtag ccgcgccggc cctgcaggtc ggtcaactgg tcgatgaatt
     3421 cgtcggcctg cgcgcgttta gcggcggtca gcatctgtgc ttcagtcgca tcagggcgtc
     3481 cgtacaggat gttgtcgcgc atcgcgcggt gcagcagcga ggtgtcttgc gtcaccatgc
     3541 cgatggcgtg gcgcaggctg tcctgcgtga ccttggcgat gtcttgatcg tcgatcagga
     3601 tgcggccctt ttgcacgtcg tgaaagcgca gcagcaggtt caccaacgtc gatttgccgg
     3661 cgcccgagcg gccgatcagg cccactttct cgcccgggtg aatggtcagg ttgaggttct
     3721 cgatcaccgg ccgcgcgccg tcgcggtagc tgaatgtgac gtcgtcaaag cgcacctcgc
     3781 cgcgcgtcac caccagtggc ttggcgcccg gcgcatccac caccgcgcgc ggccgcgaca
     3841 gcgtgttgat gccgtcctgg atggtgccca cgctctcgaa caggctggtc atctcccaca
     3901 tcacccaatg gctcatgccc gacaggcgca gcgccatcgc cgtcaccgcg gccaccgcgc
     3961 cggtgccgac ctgaccgatg gaccacaacc acagcgccgt gccgcaggcg gccaggatca
     4021 gcagcacgat gagcgcgtgg ttcaccgtct cgaagatgct gaccagccgc atctgcgcgt
     4081 agccggtctg gcggaagtct tccatcgccg cgcgggcaaa ctgcgcctcg cgccgggtgt
     4141 gtgagaacag cttgacggtg gcgatgttgg tgtacgcgtc ggtaatgcgc ccggtcatca
     4201 cgctgcgcgc gtcggcctgc gcacggctca cttttgaaag gcgcggcacg aagtaccagc
     4261 acgccaggcc gtacgcgacg gcccacagca gaaacggcag catcagccag ccgtcaaagc
     4321 ccgctgcaag cagcaggatg gtgaccatgt acacgcccat gccgatcacc acgtcggcgg
     4381 tggtgaagat catgtcgcgc accgccagcg cggtctgcat gatcttggtg gtgatgcggc
     4441 cggcaaattc gtcggcgtaa aaagccatgc tctggcccag catcaggcgg tgaaagttcc
     4501 agcgcagacg cagcggaaag ttgatggcca gtgtctggtg cttcacgatg gtctgcagcg
     4561 ccaccagcgc aatcgacgcc agcagcacgc ccgccaacag cagcagcacg ccgccgcgct
     4621 cggcccacaa ttggccgggc gccacctggc ccagccaatc gaccacgaag ccgagcagcg
     4681 cgaacaggag cgcgtcgtaa atcgacgtgc cagccgacag cagcgccatc agcccgatgt
     4741 agccgcgcaa gcccgtggtg gcgcgccaga caaaggccag aaagcccttg ggcggcagtt
     4801 caggctcggg atcggggtag ggcgggagga gtctctcgaa atggccgagc aagatgggtt
     4861 ctcagagggt gtatacgaaa tcatcgcgga aagcgattgc cccggatagg gtgttgtgca
     4921 aggcgcaaaa cgcggccgac agctagggaa gatctgcata acacccgccg cagcgtgagt
     4981 tgatgcactc gatttc
//
LOCUS       DAOMVO010000177         4993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4710_length_4993_cov_18.363913, whole genome shotgun sequence.
ACCESSION   DAOMVO010000177 DAOMVO010000000
VERSION     DAOMVO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_4993_cov_18.363913"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..617)
                     /locus_tag="PKV30_09075"
     CDS             complement(87..617)
                     /locus_tag="PKV30_09075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRL30399.1"
                     /translation="MAELATIARPYAEALFQVSRADLAATQSWLDSLAGAAGNDELLQ
                     FAANPKVNASQVFHIVTSVLQGPLPERGANFLRAVIDNDRLAALPEVARQFRQLANSV
                     SGSADALIQSAFPISPAELDSLKTTLEKRFGRRLEPSVQVDPDLIGGVRVVVGDEVLD
                     TSVKARLEQMKMALTA"
     gene            complement(648..1118)
                     /locus_tag="PKV30_09080"
     CDS             complement(648..1118)
                     /locus_tag="PKV30_09080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRL30400.1"
                     /translation="MNINATLFLQAVVFAILVWFTMKFVWPPITKALDERAQKIADGL
                     AAADKAKTELAAANQRVEKELATSRNETAVRLADAERRAQQIIEEAKVRATEEANKII
                     AAAQADAEQQVVRAREALREQVATLAVKGAEQILRKEVNPTVHADLLGRLKAEL"
     gene            complement(1156..1404)
                     /gene="atpE"
                     /locus_tag="PKV30_09085"
     CDS             complement(1156..1404)
                     /gene="atpE"
                     /locus_tag="PKV30_09085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRL30401.1"
                     /translation="MENVLGLVALACGLIVGLGAIGASIGIALMGGKFLESSARQPEL
                     MNELQTKMFVLAGLIDAAFLIGVAIALLFAFANPFTLG"
     gene            complement(1462..2343)
                     /gene="atpB"
                     /locus_tag="PKV30_09090"
     CDS             complement(1462..2343)
                     /gene="atpB"
                     /locus_tag="PKV30_09090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRL30402.1"
                     /translation="MAAETAAHGPSAGEYIQHHLHHLQKNFSFENVTQKNIIDFSLFN
                     ADSVFWSILLGALACWLLWLGARKATSGVPGRFQAAVEILSEMVENQAKGVIHNAKSR
                     KLVSPLALTVFVWIFAMNAMDLLPVDLLPLAWQGVTGDHHAYQRVVPTADLSTTLGLS
                     ISVLLVCLVYNVKIKGAGGWAHELVTAPFGAHPVLWPINFLMQIIEFVAKTVSHGMRL
                     FGNMFAGELVFMLIALMGGVWAWQFNPLSGGFWLGVGHVIAGTVWAIFHILIITLQAF
                     IFMMLTLIYVGQAHDAH"
     gene            complement(2365..2883)
                     /locus_tag="PKV30_09095"
     CDS             complement(2365..2883)
                     /locus_tag="PKV30_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRL30403.1"
                     /translation="MVTIAPEPARAPNGFADEAIDASDADFKPMSADEAREWRAAQPP
                     MSPWPVVWVQAAAGLVVVALAWAVSAGKMPVTQSTAYGALAAWLPAVFFARLVARRMR
                     LQANAGSALMALMVGEGIKVVLTVALLLAAPKVLTQVHWLALLAGFVMTIKAAWVALW
                     LMSARRRAARRD"
     gene            3076..4014
                     /locus_tag="PKV30_09100"
     CDS             3076..4014
                     /locus_tag="PKV30_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRL30404.1"
                     /translation="MTTHPADSLPPLPCYVSGEITTMPEARISPFDRGFIFGDGVYEG
                     ISIYGRPGHAPQPYRFDQHMARLERSLAETRIPNPHTRAEWRQIALDLIAAYAGQTSA
                     SGQHDAQALPQDWFYYFQVTRGVALRDHPMIEGLTPTVLATCLPMKPPTPEQRANGVA
                     CVTADDFRWKKAHIKSISLLGAVFARQISFEQGALETVMFRDGFLSEAAASNVWVVKN
                     GVVMGPPKDNLVLEGIRYGAIAELCAQEGIGFELRRIARAEVLAADELLLSSATKEVL
                     PITTLDGQPVGHGQPGPVYQKLYAAYQRAKEALFTP"
     gene            4041..4340
                     /locus_tag="PKV30_09105"
     CDS             4041..4340
                     /locus_tag="PKV30_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HRL30405.1"
                     /translation="MPADGEPIKLTPERRESLIDYPSAFPIKVVGVNGNGFVTAITHI
                     AKQFDPNFDASTIELRESGGGKYLGLTITVTATSREQLDDLYRALSTHPMAKWVL"
     gene            complement(4666..>4993)
                     /locus_tag="PKV30_09110"
     CDS             complement(4666..>4993)
                     /locus_tag="PKV30_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30406.1"
                     /translation="QGWRRGVRLVVTSSGGIGQVEVRPTRGGWPNQVQVEFRYAPDRP
                     FDALEGMRFQVINPAHTAGDEVPPLVPDNFVFWQRDGRFWLDLPSGWLRGQQGLRIAW
                     VDRYRR"
BASE COUNT          914 a         1700 c         1553 g          826 t
ORIGIN      
        1 ttgatcagtt ctgaaatttc tgcgggattg agttgcatga ctctttcctt gtcctttggg
       61 ttatcgaggg gctcttgcct gacgcatcag gcggtgaggg ccatcttcat ttgttccagg
      121 cgggccttga ccgaggtgtc gagcacctcg tcgcccacga ccacgcgcac gccgccaatc
      181 agatctggat cgacctgcac gctcggctcc agccggcgac caaagcgctt ttccagcgtg
      241 gttttcagcg aatccagctc agccgggctg atcggaaaag cgctctggat cagcgcgtcg
      301 gcgctgccgc tcacgctgtt ggccagctgg cggaactggc gcgccacttc gggcagcgcg
      361 gccagacggt cgttgtcgat cacggcgcgc aggaagttgg caccgcgctc aggcagcggg
      421 ccttgcagca cgctggtgac gatgtggaac acctggctgg cgttgacctt ggggttggcg
      481 gcaaactgca gcaactcgtc gttgcccgcc gcgcccgcca ggctgtccag ccacgattgc
      541 gtcgccgcca gatcggcgcg cgacacctga aacagggcct cggcgtacgg tctggcgatg
      601 gtggcgagtt ctgccatggg tttttccttt gcgcgtgccc tgacgaatca gagctcggcc
      661 ttcaaacggc ccagcagatc cgcgtgcacc gtcgggttga cttccttgcg caggatttgc
      721 tcggcgccct tgacggccag cgtggccacc tgctcgcgca gggcttcgcg ggcgcgcacc
      781 acctgctgct cggcatccgc ctgggcagcg gcaatgatct tgttggcttc ctcggtggcg
      841 cgcaccttgg cttcctcgat gatctgctgc gcgcggcgct cggcgtcggc caggcgaacc
      901 gcggtctcgt tgcgcgacgt ggccagttcc ttctcgacgc gctggttggc ggcggcgagt
      961 tcggtcttgg ccttgtcggc ggcagcgagg ccgtcggcga ttttctgcgc ccgctcgtcc
     1021 agtgccttcg tgatcggcgg ccacacgaat ttcatcgtga accacaccag gatcgcgaag
     1081 acaacggcct gcaggaacag ggtcgcgttg atattcacgt gtcgactcct ttcagtctcg
     1141 aaaggctaaa gccgattaac cgagggtgaa ggggttggcg aaggcgaaca gcagcgcgat
     1201 ggccacgccg atcaggaagg cggcgtcgat caggccggcc agcacgaaca tcttggtttg
     1261 cagttcgttc atcagctcgg gctgacgtgc cgacgattca aggaacttgc cgcccatcag
     1321 cgcgatgccg atggaggcgc caatggcgcc caagccgacg atgagaccac aagccagagc
     1381 gacgaggccg agaacgtttt ccatgattgc tcctagagaa agaaaagaaa aaagttaagg
     1441 gaaaggaaag aaagcgagaa ctcagtgcgc gtcgtgcgcc tgacccacgt agatcagcgt
     1501 cagcatcatg aagatgaacg cctgcagcgt aatgatcagg atgtggaaga tcgcccacac
     1561 ggtccccgca atcacgtgcc cgacacccag ccagaaaccg cccgacaggg ggttgaactg
     1621 ccaggcccat acgccgccca tcagggcgat cagcatgaag acgagttcgc cggcaaacat
     1681 gttgccgaac agtcgcatgc cgtgcgagac ggtcttggcg acgaattcga tgatctgcat
     1741 caggaagttg atcggccaca gcaccgggtg cgcgccaaac ggggcggtga ccagctcgtg
     1801 cgcccagccg ccggcgccct tgatcttgac gttgtagacc aggcacacca gcagcacgct
     1861 gatcgacagg cccagcgtgg tggacagatc ggccgtcggc accacgcgct ggtaggcgtg
     1921 gtggtcgccc gtgacgccct gccacgccag cggcagcagg tcgaccggca gcaggtccat
     1981 ggcgttcatg gcaaagatcc agacgaacac cgtgagcgcc agcggcgaga cgagcttgcg
     2041 gctcttggcg ttgtggatca cgcccttggc ctggttctcg accatttcgg acaggatttc
     2101 gaccgcggcc tgaaagcgcc ccggcacgcc cgaagtggcc ttgcgcgcgc ccagccacag
     2161 cagccagcag gcgagtgcgc ccagcaggat cgaccagaac accgagtcgg cgttgaacag
     2221 gctgaagtcg atgatgtttt tctgcgtgac gttctcgaac gagaagttct tctgcaggtg
     2281 atgcaggtgg tgctggatgt attccccagc gctcggtccg tgtgcagcgg tttcagcagc
     2341 catatgtcac cagttgttcc aacgtcagtc ccgtcgtgct gcgcggcgcc ttgccgacat
     2401 cagccacaaa gccacccagg cggccttgat cgtcatcaca aaaccagcca gcaaggccag
     2461 ccagtgcacc tgcgtgagca ccttgggcgc cgccagcagc agcgccacgg tgagcacgac
     2521 cttgattccc tcccccacca tcagcgccat caaggcactg ccggcgttgg cttgcagccg
     2581 catccggcgc gccaccaggc gggcgaaaaa caccgctggc aaccacgccg cgagggcgcc
     2641 gtaggccgtg gattgggtaa cgggcatctt gcccgccgac accgcccacg ccagagccac
     2701 caccaccaga cctgccgccg cctgcaccca gaccactggc cagggcgaca tcggcggctg
     2761 ggcagcacgc cattcacgcg cctcatcggc gctcatcggc ttgaaatcgg cgtcggaggc
     2821 atcaattgcc tcgtcggcga acccgttcgg cgcccttgcg ggttcgggtg cgattgtcac
     2881 catggcgaat tacgtgcagt ttacgtccgg caacgactgc aaagcccatg attatagggg
     2941 tttgtccgtg gtcggcggcc gcttgtccta cacgcgggcc gcggcacggc gcgcggcccg
     3001 ggagcgaccg cgtgcgcgac aatgaacgcc gcccatcgtt tcgcccattg gcgccccatc
     3061 aaagccacgc tgcccatgac cacccacccc gccgactcgc tgccgccgct gccctgttat
     3121 gtgagtggtg aaatcaccac aatgcccgag gcgcgcatca gccctttcga ccgcggcttc
     3181 atctttggcg acggcgtgta cgaaggcatc tcgatctacg gccgccccgg ccacgcgccg
     3241 caaccgtacc gtttcgacca gcacatggcg cggctcgagc gcagcctggc cgaaacgcgc
     3301 atccccaacc cgcacacgcg tgccgagtgg cgccagattg ctcttgattt gatagctgct
     3361 tacgcaggac agacaagcgc cagcggccaa catgatgccc aagcgctgcc gcaggactgg
     3421 ttctactact tccaggtcac acgcggcgtc gccctgcgcg accacccgat gatcgagggc
     3481 ctgacgccca ccgtgctcgc cacctgtcta cccatgaaac cgcccacgcc cgagcagcgc
     3541 gcgaacggcg tggcctgcgt cacggccgac gacttccgct ggaagaaggc gcacatcaag
     3601 tcgatcagcc tgctgggcgc ggtgttcgcg cgccagatca gctttgagca gggcgcgctc
     3661 gaaaccgtga tgttccgcga cggcttcttg agcgaagccg ccgccagcaa cgtctgggtg
     3721 gtgaaaaacg gcgtcgtcat gggcccgccg aaggacaacc tggtgctgga gggcattcgc
     3781 tacggcgcca tcgccgagct gtgcgcgcaa gagggcatcg gcttcgagct gcgccgcatt
     3841 gcgcgcgccg aggtgctggc ggccgatgag ttgctgctgt cctccgccac caaggaggtg
     3901 ttgccgatca ccacgctcga cggccagccg gtcggccacg gccagcccgg cccggtgtac
     3961 cagaagcttt acgccgccta ccaacgcgcc aaggaggcgc tgttcacccc atgaccacac
     4021 ccgtcccgcc cctgcccgac atgcccgctg acggcgagcc gatcaaactc acgcccgagc
     4081 ggcgcgaatc gctgatcgac taccccagcg ccttcccgat caaggtggtc ggcgtcaacg
     4141 gaaacggctt cgtgaccgcg atcacgcaca ttgccaagca gttcgacccc aacttcgacg
     4201 ccagcaccat cgagctgcgc gaaagcggcg gcggcaagta cctgggcctg accatcaccg
     4261 tcaccgccac cagccgcgag cagctcgatg acctgtaccg cgcgctcagc acgcacccga
     4321 tggcgaagtg ggtgctttga cccccctgtg gcgctgacgc gccctctcgc aggggacgcc
     4381 gctggcggcc ggtgcaagcc cggccgctgc ggcccacaac gtggccggct gcgcggccac
     4441 tgaaaattga tggacagatc aagaaacgat cccacgccgt tcgtcctgag cctgtcgaag
     4501 gacttcgtgc cacacacccg ggcttcgaca agctcagccc gaacggatga tgggctgtcg
     4561 gactattcat cagtccagcc ataggtaaat tgcggcccca gcgcttgccc accaaacgcg
     4621 agcagctata tttttcatat cgcacgccgt tgggggcgga tcgcctcaac gccgataccg
     4681 atccacccac gcgatgcgca agccctgctg gccgcgcagc cagccgctgg gcagatcgag
     4741 ccagaagcgg ccgtcgcgct gccagaacac gaagttgtcg ggcaccagcg gcggcacctc
     4801 gtcgcccgcc gtgtgcgccg ggttgatgac ctgaaagcgc atgccctcca gcgcgtcgaa
     4861 cggccggtcg ggcgcgtagc ggaactccac ctgcacctgg ttcggccagc cgccgcgcgt
     4921 gggccgcacc tcgacctggc cgatgccgcc gctggacgtc accaccagcc gcacgccgcg
     4981 gcgccagcct tgg
//
LOCUS       DAOMVO010000178         4983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4724_length_4983_cov_14.180398, whole genome shotgun sequence.
ACCESSION   DAOMVO010000178 DAOMVO010000000
VERSION     DAOMVO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_4983_cov_14.180398"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKV30_09115"
     CDS             <1..550
                     /locus_tag="PKV30_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL30407.1"
                     /translation="DFAVRTRVWKEARDLVYNVINCDKPIVSAMHGPAVGAGLVAGLL
                     ADISIAAKSAKIVDGHTRLGVAAGDHAAICWPLLCGMAKAKYYLMLCEAVTGEEAERI
                     GLVSLAVEDDQLLDKAYEVADKLAAGSATAIRWTKYSLNNWYRQAGPSFDTSLALEFM
                     GFAGPDVQEGVASLRERRAPKY"
     gene            complement(614..763)
                     /locus_tag="PKV30_09120"
     CDS             complement(614..763)
                     /locus_tag="PKV30_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRL30408.1"
                     /translation="MIGSALDVQRGRLLCSVFDAEHLADGPIAQGHLERVMPMGLHAS
                     FIRRT"
     gene            complement(764..2122)
                     /locus_tag="PKV30_09125"
     CDS             complement(764..2122)
                     /locus_tag="PKV30_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HRL30409.1"
                     /translation="MDRRDFTRHLLGAAAGLTGAAAPWSALARTPAPAARFIPWPRAF
                     DGLQADVPRRAMALRGRVPVALAGGVLLRNGPARHQLGGVRTSHWFDGDGMIQRYAFG
                     SQGITHEGRFVQTPKFVAESAAGRWLEPAFATHPKDAPPPPSPDEMNVANTHIVAHGG
                     EVLALWEGGSAMKLDADTLDARGFKTWAPDYAGMPLSAHPRVEPDGTLWSFGVSIGAG
                     VLSIYRVSADGRDVKTHSLKVPDIGMLHDFAVTGRHLVFLLPPFVYDIERAKAGETFL
                     DSHVWRPSLGMRVLLLPKDELDAPRWLQLPAGFVFHLGNAWEDAQGVIRLDYVRSPDA
                     TLVLQGARDLMRGAVNGNAPAARVARVRLDPASGNARQEELPLDAEFPRIDPRRTGRR
                     YRHVYPAERVDAPLGRPLFDSVTRLDVDTGRVQRHRYGAGVLAEEHVFVPRPGSAREG
                     EG"
     gene            complement(2132..2557)
                     /locus_tag="PKV30_09130"
     CDS             complement(2132..2557)
                     /locus_tag="PKV30_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HRL30410.1"
                     /translation="MVFASFVCRAAGALALAAACSAHAADLTLTIEGVNAAEGRVAAA
                     AYDSAGSWLKGPPAAVAQSDSASPRTVLVLRDVPPGRYGVSVMHDRNANGKLDSNIVG
                     IPTEPYGAIRHARGRMGPPAFEDAAVDVQGDTALTIHLH"
     gene            complement(2708..3358)
                     /locus_tag="PKV30_09135"
     CDS             complement(2708..3358)
                     /locus_tag="PKV30_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRL30411.1"
                     /translation="MLKLCGFAASNYYNKVKLALLEKGVPFEEELAWIGETDPAASPI
                     GKVPYALTDDGPLSESTILLEYIEAKYPQHPLLPADAYAAARVRELVRYIELHLELVA
                     RNLYPEAFFGGKVSEGLKDKLKPQLEKHIAGFGQLAKFSPFIAGDTFTLADCAAAVHL
                     PLVASATKIVYGQDMLAGLPVKDYLKRIGERPAVQQVNADRKTNTEQMLARMKAKG"
     gene            3445..3804
                     /locus_tag="PKV30_09140"
     CDS             3445..3804
                     /locus_tag="PKV30_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30412.1"
                     /translation="MLPRRLHHLIARLALAWFALALGVAAASPLVQPRAMQLVCSAEG
                     GVKLMLVQPGEGGVDVGTHQLDCALCLIGNAPPPPRVGAVLDMAPLMQAPATAYADAP
                     RHAQAAAPPPARGPPLL"
     gene            3912..4400
                     /locus_tag="PKV30_09145"
     CDS             3912..4400
                     /locus_tag="PKV30_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRL30413.1"
                     /translation="MITTTPFKTLAAALLLAATTTHALAQDAVKVEGAWARASVPGQK
                     ATGAFMTLTAPAATQLVGVSSPAAGVAEVHEMAMQGDVMKMRAIESLALPAGQPVQLK
                     PGGHHIMLLDLKAPLAEGATVPLTLLFKDAAGKPGKMELQVPVRALASPAAPAGGHGH
                     KH"
     gene            4498..>4983
                     /locus_tag="PKV30_09150"
     CDS             4498..>4983
                     /locus_tag="PKV30_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30414.1"
                     /translation="MSDANAFDFGKFIPGFDFLQKLSASARGAGGAAPGLHSWVAPTV
                     SVDELDKRIKELKAVLFWLEQNGTALKATIQALEVQKMTLAALAGMNLNMAEVAKAFT
                     VPTGGAGTTAAPPAEPPKPEGWPYATAPMPASATQPVEEDPAAAPAEPPAEAAPAPAP
                     PP"
BASE COUNT          736 a         1701 c         1797 g          749 t
ORIGIN      
        1 cgacttcgcc gtgcgcaccc gtgtgtggaa ggaggcgcgc gacctggtct acaacgtcat
       61 caattgcgac aagcccatcg tcagcgccat gcacggcccg gccgtcggcg cggggctggt
      121 ggcggggttg ctggccgata tttcgattgc cgccaagtcg gccaagatcg tcgacggcca
      181 cacgcgcctc ggcgtggcgg ccggcgatca tgccgccatc tgctggccgc tgctgtgcgg
      241 catggccaag gccaagtact acctgatgtt gtgcgaggcg gtgacgggcg aggaggccga
      301 acgcatcggc ctcgtctcgc tggcggtgga ggacgaccag ttgctggaca aggcctacga
      361 agttgccgac aagctggccg ccggcagcgc gacggcgatc cgctggacca agtactcgct
      421 caacaactgg tatcggcagg cgggaccgag cttcgacacc tcgctggcgc tggaattcat
      481 gggctttgcc gggccggacg ttcaggaagg cgtggcgagc ttgcgcgagc ggcgtgcgcc
      541 caagtattga cgaatgctat ttattagata gctgctcgcg cttgtccatc aagcgctgac
      601 ggcatttttt tcttcaagtc cgccggatga agctggcgtg cagccccatc ggcatgacgc
      661 gctccagatg gccctgcgcg atcggtccgt cggccaggtg ctcggcgtca aacaccgagc
      721 acagcaggcg cccgcgctgt acatccagcg cgctgccgat cacctagcct tcgccttcgc
      781 gcgcgctgcc cgggcgcggc acgaagacgt gttcctcggc cagcacgccg gcgccgtagc
      841 ggtgtcgctg cacgcggccg gtgtcgacgt cgagccgcgt cacgctgtcg aacagcggcc
      901 ggccgagcgg cgcatcgacg cgctcggccg ggtagacgtg gcggtagcgc cggccggtgc
      961 ggcgcgggtc gatgcgcggg aattcggcgt ccagcggcag ttcctcctgc cgcgcgttgc
     1021 cgctggccgg atcgagccgc acgcgcgcca cgcgggcggc gggcgcgttg ccgttcaccg
     1081 cgccgcgcat caggtcgcgc gcgccctgca gcaccagcgt ggcgtcgggc gagcgcacgt
     1141 agtcgaggcg aatcacgccc tgcgcgtctt cccaggcgtt gcccaggtga aacacgaaac
     1201 cggccggcag ctgcagccag cgtggcgcat ccagctcatc tttcggtagc aacagcacgc
     1261 gcatgccgag cgacgggcgc cagacgtgac tgtccagaaa ggtttcgccg gccttggcgc
     1321 gctcgatgtc gtagacgaac ggcggcaaca gaaagaccag gtgccgcccg gtcacggcga
     1381 agtcgtgcaa catgccgatg tccggcacct tcaggctgtg cgtcttcacg tcgcggccgt
     1441 cggccgacac gcggtagatc gacagcacgc cggcgccgat ggagacgccg aagctccaca
     1501 gcgtgccgtc cggctccacg cgcgggtgcg ccgaaagcgg catgccggcg tagtcgggcg
     1561 cccaggtctt gaagccgcgc gcgtccagcg tgtcggcgtc cagcttcatg gccgatccgc
     1621 cttcccacag cgccagcacc tcgccgccat gcgcgacgat gtgggtgttg gccacgttca
     1681 tctcgtcggg cgacgggggc ggcggcgcgt ccttggggtg ggtggcgaag gccggttcca
     1741 gccagcggcc ggcggcgctt tcggcgacga acttcggggt ctgcacgaag cggccttcgt
     1801 gcgtgatgcc ttggctgcca aaagcgtagc gctggatcat gccgtcgccg tcgaaccagt
     1861 ggctggtacg cacgccccca agctggtggc gcgccgggcc gttgcgcagc agcacgccac
     1921 cggccagcgc gacgggcacg cggccgcgca gcgccatcgc gcggcgcggc acatcggcct
     1981 gcaacccgtc gaaggcgcgg ggccacggaa tgaatcgggc ggccggggcc ggcgtgcgcg
     2041 ccagcgcgct ccagggcgcg gcggcgccgg tcagcccggc agcggcgcca agcaggtggc
     2101 gggtgaagtc gcggcggtcc atggcgggct ttcagtgcag gtggatggtc agcgcggtgt
     2161 cgccttgcac gtcgacggca gcatcctcga aggcgggcgg gcccatgcgg ccgcgtgcgt
     2221 ggcggatggc gccgtagggt tcggtcggaa tgccgacgat gttgctgtcg agcttgccgt
     2281 tggcgttcct gtcgtgcatc acgctcacgc cgtaacggcc gggcggcacg tcgcgcagca
     2341 ccagcacggt gcgcgggctg gcggaatcgg actgcgccac ggccgcgggc gggcccttga
     2401 gccagctgcc ggcgctgtcg taggcggcgg ccgcgacgcg gccttcggcg gcgttgacgc
     2461 cctcgatggt gagggtcagg tcggcggcgt gggccgaaca tgcggcggcc aacgcgagcg
     2521 cgccagcggc gcggcagacg aaagaagcaa aaaccatggc gaacctcctg gggcagtgat
     2581 cggtgggcgg ggccggctga accggcccta ggggatcact gtcggggttc gtgcgcgcgc
     2641 tggcgagcgc cgtgggacga aatgcgcaac gggcggcgcc aaatgccgcc cgcgccggcg
     2701 gggcgtgtca gcccttggcc ttcatccgcg ccagcatctg ctcagtattg gtcttgcgat
     2761 ccgcattcac ctgctgcacc gccgggcgct cgccgatgcg cttgaggtag tccttcaccg
     2821 gcaggccggc cagcatgtct tggccataga cgatcttcgt ggcgctggcc accagcggca
     2881 ggtgcacggc ggcggcgcag tcggccaggg tgaaagtgtc gccggcgatg aagggcgaga
     2941 acttggccag ctggccgaag ccggcgatgt gtttctcaag ctggggtttc agcttgtctt
     3001 tcagcccctc gctcaccttg ccgccaaaaa aggcctcggg gtacaggttg cgcgccacca
     3061 gttccaggtg cagctcgatg tagcgcacca gctcgcgcac gcgggcggcg gcgtaggcgt
     3121 cggcgggcag cagcgggtgt tgcgggtact tggcttcgat gtattccagc aggatggtcg
     3181 attcggacag cgggccgtcg tcggtgaggg cgtagggcac cttgccgatg gggctggcgg
     3241 ctgggtcggt ctcgccgatc caggccagct cttcctcgaa cggcacgcct ttttccagca
     3301 gcgccagctt gaccttgttg tagtagttgc tggcggcaaa gccgcagagc ttgagcattc
     3361 ggtgtctcct cgggtgggca aagagcgcga gcgtagcgcg gcagcggcgc ggcggcagtc
     3421 gccgagcgga ccataatccc gcccatgctg ccgcgccgcc tgcaccacct cattgcccgc
     3481 ctggcgctgg cgtggtttgc gcttgcgctg ggcgtggccg ccgcgtcgcc gctggtgcag
     3541 ccgcgcgcca tgcaactggt gtgcagcgct gagggcggcg tcaagctgat gctggtgcag
     3601 ccgggcgaag gtggggtcga cgtcggcacc caccagctcg attgcgcgct gtgcctgatc
     3661 ggcaacgcac cgccgccgcc acgggtgggc gcggtgctgg acatggcgcc gctgatgcaa
     3721 gcgccagcca ccgcgtacgc cgatgcgcca cgtcacgccc aagccgccgc gccgccgcct
     3781 gcgcgcgggc cgccgcttct ctgatgcttt tgttttgata gctgccagcg cttgatatac
     3841 aagcactggc ggcctttttg attcatagac gcggccgtgt gccgccgttg ctggcattgg
     3901 agagagagca catgatcacc accacccctt tcaagaccct ggccgccgcc ttgctgctgg
     3961 ccgccaccac cacgcacgcg ctggcgcagg acgccgtcaa ggtcgagggc gcctgggcgc
     4021 gcgcctcggt ccccgggcag aaggccaccg gcgccttcat gacgctcacg gcgcccgccg
     4081 ccacgcagct ggtcggcgtc agctcgcccg ccgccggcgt ggccgaggtg cacgagatgg
     4141 ccatgcaggg cgacgtgatg aagatgcgcg ccatcgaatc gctggcgctg ccggccggcc
     4201 agccggtgca gctcaagccg ggcggccacc acatcatgct gctcgatctg aaggcgccgc
     4261 tggccgaggg tgccacggtg ccgctgacgc tgctgttcaa ggacgcggcg ggcaagcccg
     4321 gcaagatgga attgcaggtg ccggtgcgcg cgctcgccag cccggccgcc cccgccggcg
     4381 gccacggcca caagcactga gcggcgcggc aggcccgccc agcgctgccg aaaagggagc
     4441 gcgccagccg ccgctttcgg gtaagctggg cgcacccctg ttcagaaagg cgctgccatg
     4501 agtgatgcca acgcgttcga tttcggcaag ttcatccccg ggttcgactt tttgcagaag
     4561 ctgtccgcca gcgcgcgggg cgcgggcggc gcggcccccg ggctgcacag ctgggtggcg
     4621 cccaccgtca gcgtcgacga gctcgacaag cgcatcaagg aattgaaagc ggttctgttc
     4681 tggctggagc agaacggcac cgcgctcaag gccaccatcc aggcacttga agtgcagaag
     4741 atgacgctgg ccgcgctggc cggcatgaac ctgaacatgg ccgaggtcgc caaggccttc
     4801 accgtgccca cgggcggcgc tggcaccacg gccgcgccgc cggccgaacc gcccaagccc
     4861 gaaggctggc cgtacgccac cgcgcccatg ccggcgtcgg cgacccaacc ggtggaagaa
     4921 gaccccgccg ccgcgcccgc cgagccgccg gccgaagccg cgcccgcccc cgcgccgcca
     4981 cct
//
LOCUS       DAOMVO010000179         4972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4740_length_4972_cov_11.522270, whole genome shotgun sequence.
ACCESSION   DAOMVO010000179 DAOMVO010000000
VERSION     DAOMVO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_4972_cov_11.522270"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /gene="orn"
                     /locus_tag="PKV30_09155"
     CDS             <1..474
                     /gene="orn"
                     /locus_tag="PKV30_09155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HRL30415.1"
                     /translation="EIAVVVTGPDLTPRVEGPVLVIHQPDSVLDAMDTWNKGTHGRSG
                     LIDKVKASTLTEDEAVDQLIAFLSQYVPKGCSPMCGNTIGQDRRFLVKYMPRLEAFFH
                     YRNIDVSTLKELARRWRPEVVNGFKKQQSHTALADVHESIDELAHYRAHFIRLAD"
     gene            complement(500..1210)
                     /locus_tag="PKV30_09160"
     CDS             complement(500..1210)
                     /locus_tag="PKV30_09160"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRL30416.1"
                     /translation="MIFILVAVGLLAAGLGLTTYLLRRGDGKRRSAAAKRRLPAQWPL
                     NPRPLANSGERHVWHWLRQTFPEHHVMVKLPVTRFTMPRAPGEGQEWFELLAGAYCSF
                     SVCDDNGRVLGCVDVMGPRGLSRGNRQLKQTLLSQCGIGYWVISAESLPDPDRLRADF
                     LGTPAPDSLPPQSEQARLEAVRHRLHESLDRKRSRRHNHVLEAVDQAEKDSEITPWPQ
                     ADSFLGTLDSRRSELEKV"
     gene            1414..2145
                     /locus_tag="PKV30_09165"
     CDS             1414..2145
                     /locus_tag="PKV30_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRL30417.1"
                     /translation="MRLSELLYSQGFGTRRLCAGLIENGLVTVRGEVLSEPATEIDVS
                     TWPVAYTVQGQSWEYQQRAYVMLNKPAGYECSHRPGAWPSVYTLLPAPLRQRPTPGKV
                     QGVQAVGRLDQDTTGLLLLSDDGAFIHRMNSPRHHVPKVYEVTTKHPLDERQITRLKS
                     GVVLDDDPKPVAAADAQALGEHHLQLTLTEGKYHQVKRMVAAVGNRVEALHRSRIGGL
                     ALPADLAPGKWRWLGEDELASLKTG"
     gene            2647..>3012
                     /locus_tag="PKV30_09170"
     CDS             2647..>3012
                     /locus_tag="PKV30_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30418.1"
                     /translation="MTWGQPVGSLDGVVRRVFAAAGARFLQHHVDRLGGADAHRGSAA
                     RRAEQAQLQLGARRQAGVGADFQPLDLRPCRRGGALRVGGAGHHQGAGVDQQGGGRAD
                     GLRQGRDRTKKCVHVGSPRK"
     assembly_gap    3013..3112
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3264..4046)
                     /locus_tag="PKV30_09175"
     CDS             complement(3264..4046)
                     /locus_tag="PKV30_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL30419.1"
                     /translation="MQTVDIEGVFLEVAHIAAPAGADAAQAPLVFLHEGLGSVAMWRD
                     WPASLCADTGRAGWVYSRRGYGRSSPVADVRGAGRLQHDYMHREALDVLPKLLARLGL
                     QRPVLVGHSDGSTIALIHAAAHPVSACVVMAPHVMVEDVSVASIDEARLAYERDGLRE
                     RLARYHDDVDVAFWQWNDVWLSPAFRDFDIRGEIGAIGAPLLAIQGIQDQYGTMAQID
                     EVARAVPQTELVKLPDCGHSPHRDQPQAVNAAIAAFLHRLQG"
     gene            4183..>4972
                     /locus_tag="PKV30_09180"
     CDS             4183..>4972
                     /locus_tag="PKV30_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRL30420.1"
                     /translation="MTALPLLPARAATPSRRGAPVFALNSLDGDVSVIDSITWTEIKR
                     IPTGREPHHLYLTPDEKSVIVANAGSDSLTFIDPRTAEVQRTLRGIRDPYHLRFSPDM
                     KWFVTAANRLNHIDIYSWDGSNPTLAKRIPTGKTPSHLWIDTQSTTAWSSMQDSDELV
                     VVDLDTQQFTHRVKTGPMPADVFVTPDDKHLLLGLTGGDGVEVYDVAGGKAPELVKKI
                     ATGKGAHAFRAAGDGRHVFVSNRVANTISKIDYVDFKAVDTFPAP"
BASE COUNT          827 a         1638 c         1635 g          772 t
ORIGIN      
        1 gagatcgccg tcgtcgtcac cgggcccgac ttgacgccgc gcgtcgaagg cccggtgctg
       61 gtgatccacc agcccgacag cgtgctggac gccatggaca cctggaacaa gggcacgcac
      121 ggccgcagcg gcttgattga caaagtgaag gcctccacgc tgaccgagga cgaagccgtc
      181 gaccagctga tcgccttctt gtcgcagtac gtgcccaagg gctgctcgcc catgtgtggc
      241 aacaccatcg ggcaggaccg gcgctttctg gtcaagtaca tgccgcggct ggaggcgttt
      301 tttcactacc gcaacatcga tgtcagcacg ctgaaagagc tggcgcgccg ctggcggccc
      361 gaggtggtga atgggttcaa gaagcagcag tcgcacaccg cgctggcgga cgtgcacgag
      421 tcgatcgacg aactggcgca ctaccgggcg cacttcatcc ggctggcgga ttgagcgcag
      481 cgcgagcgcc ggggtgtcgt cagacttttt ccagctccga gcgccggctg tcgagcgtgc
      541 cgaggaagga atcggcctgc ggccacggcg tgatttccga atccttctcg gcctgatcga
      601 ccgcttcgag cacatggttg tgccggcggc tgcgcttgcg gtcgagcgat tcgtgcagcc
      661 ggtggcggac ggcttcgagc cgggcttgct cgctttgcgg cggcagcgaa tcgggggccg
      721 gcgtgccgag gaaatcggcg cgcagccggt ccgggtcggg cagggattcg gccgatatca
      781 cccaatagcc gatgccgcac tgcgacagca gggtctgctt gagctgccgg ttgccgcgcg
      841 acaggccgcg cgggcccatg acgtcgacgc aaccgagcac gcggccgttg tcgtcgcaca
      901 cgctgaaact gcaataggcg ccggccagca gctcaaacca ttcctggcct tcgccgggcg
      961 cgcggggcat ggtgaagcgc gtcacgggca gcttcaccat cacatgatgc tcagggaaag
     1021 tctggcgcag ccagtgccag acgtgccgtt cgccactgtt ggcgagcggc cgggggttga
     1081 gcggccattg cgccggcagc cgacgtttgg ccgccgcgct gcgccgtttg ccatcgccac
     1141 gccgcagcag gtaagtggtc agtcccaacc cggcagcgag caagcccact gcgaccagaa
     1201 tgaagatcaa aatcccggtc gaaccggtgg atggactcaa cgcttgctcc cttgcgcgtg
     1261 ttcgcgctgt atctatttgc caagtgttac ataagtatca atggaagtgg taggcgccaa
     1321 acctctactt ccgaaggtcg atgaccatac tgccaaaaat gtcatagagg gtgccatacc
     1381 gatcacccta tccatacctt cttttggtga tgcatgcgcc tgagcgaact tctgtattca
     1441 caaggttttg gcacgcgccg gctgtgcgcg gggctgatcg aaaacggcct ggtaacggtg
     1501 cgcggcgagg ttttgagcga accggccacc gaaattgacg tgtcgacatg gcccgtggcc
     1561 tacaccgtgc agggacagtc ctgggagtat cagcaacgcg cttacgtcat gctgaacaaa
     1621 ccggcgggct acgagtgctc gcaccgaccc ggcgcctggc cgagcgtcta cacgctgctg
     1681 ccggcgccgt tgcgccagcg gccgacgccg ggcaaagtac agggtgtgca ggcggtcgga
     1741 aggctggatc aggacaccac cggcctgctg ctgctgtcgg acgacggcgc cttcatccac
     1801 cgcatgaatt cgccgcgcca ccacgtgccc aaggtgtacg aggtgaccac caagcaccca
     1861 ctggacgagc ggcagatcac ccgcctgaaa tccggcgtgg tgctggacga cgaccccaaa
     1921 ccggtcgctg ccgccgacgc ccaagcgctc ggcgaacacc atttgcagct gacgctgacc
     1981 gagggcaagt accaccaggt caagcgcatg gtggcggcgg tgggcaatcg ggtcgaggcg
     2041 ctgcaccgct cgcgcatcgg cggtctggcg ctgccggccg atctggcgcc tggcaagtgg
     2101 cgctggcttg gtgaggacga attggccagc ctcaagaccg gctgagcacc ctgtgggttg
     2161 atttgaaggt gtttatatcg tcttgcgcag gtccagcata cgcaagcagc tatcaataca
     2221 atagtattcg acgaccgtca tggccgatca ggcggctgcc tgaccggcca ttacgcgcct
     2281 cggcgctgcg tcacgcgcag gcgcgcctcg tcgctgcggc cgtcgcggct ggcggtcacg
     2341 accacgtcgt agcgcgagcc cggcgcgccg gccggcatga actgaaagct gaagttgccg
     2401 gcggcgtcgg ccgtcagtgc aggttggcgg caatgccgtt gatgtcaaaa ctggcttgcg
     2461 cacccggcgc gccctgcacg gtgaacaccc gttccgtgcc cggcgacagg cggggcacgg
     2521 gcgcggcggc aaagctgtcg atgcgcagcg tgcggccggc agcggcgtgc cgccaccggc
     2581 cgacgccgga atggtgtagc tggcgctatt ggtcagcttg ccgcgccgca aggtggcgcg
     2641 cacggtttga cctggggcca accggtcggt tcgcttgatg gtgtagtacg ccgcgtattc
     2701 gccgctgccg gcgcccgttt ccttcagcac cacgttgatc gtcttggcgg cgctgatgcg
     2761 caccgaggca gtgccgcgcg gcgtgccgaa caggcgcagc tccagctcgg cgcccggcgc
     2821 caggccggcg tcggagccga tttccagccg ctcgatctgc ggccgtgccg gcgcggcggc
     2881 gcgctgcgcg tgggcggcgc tgggcaccac cagggtgccg gcgtcgatca gcagggcggc
     2941 gggcgtgccg acggcctgcg gcaaggccgc gacaggacga agaagtgcgt gcatgtggga
     3001 tctccccgca aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggcaag
     3121 gccgcgacag gacgaagaag tgcgtgcatg tgggatctcc ccgcaaatga tggtggccgc
     3181 acgataggcc gccaccgggc gcggccgcgt cagcgccggc cttcacgccg tttgggatgg
     3241 ggcgtcggcc gtcagccggc gaatcagccc tgcaggcggt gcaggaaggc agcgatggcg
     3301 gcgttcaccg cctgcggctg gtcgcggtgc ggcgagtggc cgcagtcggg cagtttgacc
     3361 agctcggtct gcggaacggc gcgcgccacc tcgtcgatct gggccatggt gccgtattga
     3421 tcctgaatgc cttgaatcgc cagcagcggc gcgccgatgg cgccaatttc gccccggatg
     3481 tcgaagtcgc ggaaagcggg cgacagccag acgtcgttcc attgccagaa ggccacgtcc
     3541 acgtcgtcgt ggtagcgcgc caaccgctcg cgcaggccat cgcgctcata ggccaggcgc
     3601 gcttcgtcga tcgaggcgac cgacacatcc tcgaccatca cgtgcggcgc catcaccacg
     3661 caggcgctga ccgggtgcgc ggcggcgtgg atcagggcga tggtgctgcc atctgaatgg
     3721 ccgaccagca ccggccgctg cagccccaac cgcgccagca gcttcggcag cacgtccagc
     3781 gcctcgcggt gcatgtagtc gtgctgcagg cggcccgcgc cgcgcacgtc ggccaccggg
     3841 ctggatcgtc cataaccccg gcgcgaatac acccagccgg cgcggcccgt gtcggcgcac
     3901 aggctggctg gccagtcgcg ccacatggcg accgagccca ggccttcgtg cagaaacacc
     3961 agcggcgcct gcgccgcatc ggccccggcc ggcgccgcga tgtgcgccac ctcaagaaaa
     4021 acgccctcaa tgtccaccgt ttgcatggct gggatgttat gtcagctgtc ccggcgccgc
     4081 aggccagggc tacactcgtt gatcgccctg gaacctgctg tcgtgccacc tatttcccgc
     4141 cacttttccg ccacccggcg ccgcaccttg agcctgctcg ggctgacggc gctgccgctt
     4201 ttgccggcgc gcgcggccac gccatcgcgc cgcggggcgc cggtgttcgc gctcaattcg
     4261 ctcgacggcg acgtcagcgt catcgattcg atcacctgga cggagatcaa gcgcatcccc
     4321 accggccgcg agccgcacca cctgtacctg acgccggacg aaaaatcggt catcgtcgcc
     4381 aacgccggca gcgattcgct caccttcatc gacccgcgca cggccgaggt gcagcgcacg
     4441 ctgcgcggca tccgcgaccc gtaccacctg cgcttttccc ccgacatgaa gtggttcgtc
     4501 acggcggcca accgcctcaa ccacatcgac atctacagct gggacggcag caaccctacg
     4561 ctggccaagc gcatccccac cggcaagacg ccgagccatt tgtggatcga cacccaaagc
     4621 accaccgcct ggtcgagcat gcaggacagc gacgagctgg tggtggtcga tctggacacg
     4681 cagcagttca cgcaccgcgt caagaccggc cccatgccgg ccgacgtgtt cgtcacgccg
     4741 gacgacaagc acctgctgct cggcctcacc ggcggcgacg gcgttgaggt gtacgacgtg
     4801 gccggcggca aggcgcccga gttggtcaag aagatcgcca ccggcaaggg cgcgcacgcc
     4861 tttcgcgcgg cgggcgacgg gcggcacgtg tttgtcagca accgcgtggc caacacgatc
     4921 agcaagatcg actacgtgga tttcaaggcg gtcgacacct ttcctgcgcc gt
//
LOCUS       DAOMVO010000180         4958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4764_length_4958_cov_12.294310, whole genome shotgun sequence.
ACCESSION   DAOMVO010000180 DAOMVO010000000
VERSION     DAOMVO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4764_length_4958_cov_12.294310"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKV30_09185"
     CDS             complement(<1..260)
                     /locus_tag="PKV30_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30421.1"
                     /translation="MNDIAVGASPKSAGAVLEAQSITKRFHEGPLDVTVLHGVDLTVR
                     PGETLAIVGQSGSGKSTLLHLMGGLDAPTSGSVRLMGQDFSGL"
     gene            complement(253..1521)
                     /locus_tag="PKV30_09190"
     CDS             complement(253..1521)
                     /locus_tag="PKV30_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRL30422.1"
                     /translation="MSLSQRLPYEWLLGWRYTRAGRATRRNGFISFISAASMLGIALG
                     VAALIIVLSVMNGFQKDVAGRMLSVISHVEVFSPDGAALPDPQRTLNEVRANPEVIGA
                     APFIGAQGLLARGETMQGAIVRGIDPAMEPNVTDVAAELKDTLLPKLVPGEFGILLGA
                     DLARKLGVVAGDQVTLIAPSGQVTPAGVVPRLKQMTVVGTFSSGHYEYDSALAMLNIE
                     DAARIFRLEGPTGIRLKLRDLHHARQVADQLAGSLSGHLLILDWTRQNRTWFAAVQLE
                     KRMMFIILTLIVAVAAFNLVSTLVMTVQDKRADIAILRTLGASPASIMKTFIVQGALV
                     GVIGTLAGLALGLLIAFNIDVIVPFLEKVFRASFLPKDIYLISKMPSDPQAADIVPIA
                     VISLLLAFVATIYPSWRASRVNPAEALRYE"
     gene            1599..2546
                     /locus_tag="PKV30_09195"
     CDS             1599..2546
                     /locus_tag="PKV30_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HRL30423.1"
                     /translation="MPPAPEPFHLLIADAAPLLADGAAPPPLPALPQLDALLKRLRVT
                     ETIACDDDAPDTPLERALARAHGLPGAPGQVPWAAFETGTVGTPCAWLRPCHWQLGMD
                     HITLLDPALLDLSEAESRALLAAVQPLLQEDGIALRYVRPDAWLAEGELFRSLATSSM
                     ARAAQQPLTRDALAAAPTPAQSAHLRRLQNELQMLLYTQPVNDAREQAKRWPVNALWI
                     EGAGLLDAPTPQNPGVRVESRLAPAATPDLAAWQAAWQAIDADSVAQLGRQLASGADV
                     RLTLCGPRRALTLRPATGLGARVSSLFSPLRLSDLRNQL"
     gene            2646..4355
                     /gene="recJ"
                     /locus_tag="PKV30_09200"
     CDS             2646..4355
                     /gene="recJ"
                     /locus_tag="PKV30_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRL30424.1"
                     /translation="MKIEVRDVPPRAAWALEQAGVHPLLARLFAARGVQSAADLDDAL
                     ARLLPPAGLKGAREAAVLLADAIAAQKRLCIVADYDCDGATACAVGVRGLRLLGAPHV
                     DYLVPDRVVDGYGLTPPIAERVATQGADVLITVDNGIASVDGVARARALGLAVLVTDH
                     HLPGAELPGADVIVNPNQPGCEFESKSIAGVGVMFYVLLALRAELRARGVFDAASQPK
                     LDALLPLVALGTVADVVRLDANNRRLVAQGLKRIRAGQLPVGLYALFSVASRASQKAT
                     TFDFGFALGPRINAAGRLADMTLGIELLLTDDAARAAELAATLDGINRERREIEGGMR
                     EQAMQIAEGLFDPDAVPPSALAVFDPDFHEGVVGIVAARLKDRLHRPTFVFAASHAPG
                     QAHVLKGSGRSIAGFHLRDALDLVAKRHPGVLVKFGGHAMAAGCTIAEEHFDTFDRAL
                     QQVAAEWLDAATLTRRLETDGPLPPEYRRPDMVDTLHAAVWGQGFAPPVFSEEVEVVS
                     QRLVGEKHLALKLRHQGQPIDGIWFGHTEPLPARALLAFRLEADEWQGVRRVRLLVEG
                     VAG"
     gene            complement(4495..>4958)
                     /locus_tag="PKV30_09205"
     CDS             complement(4495..>4958)
                     /locus_tag="PKV30_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRL30425.1"
                     /translation="LKHQGGFDAQHIAYKGSAPAMNDLLGGQVPVMFSDLVAALPHIK
                     SGKLRALAVGSPRRVDIVPDVKTVAEQGFKGFEAVSIGAMMAPKGTPAAAIDRMSSEL
                     KAVLAQKDVQDRLVGAGCYAHYMTPAETGRYVTAEFDKWGKVIKDNKLTVE"
BASE COUNT          765 a         1767 c         1632 g          794 t
ORIGIN      
        1 aggccactga agtcctgccc catcagccgc acgctgccgc tggtgggcgc atccagccca
       61 cccatcaggt gcagcaacgt gctcttgccc gagcccgact ggccgacgat ggccagcgtt
      121 tcgcccggcc gcaccgtcag gtcgacgccg tgcagcacgg tcacatccag cgggccttcg
      181 tgaaagcgct tggtgatgga ttgcgcctcc aatacggcgc cagcgctttt tgggcttgcg
      241 ccaacagcta tatcattcat agcgcagcgc ctctgccgga ttgacgcggc tggcgcgcca
      301 gctcggataa atcgtcgcca caaatgccag cagcagcgag atcaccgcaa tcggcacgat
      361 gtcggccgcc tgcgggtcgc tcggcatctt gctgatcagg tagatgtcct tgggcaaaaa
      421 gctggcgcgg aacacttttt caagaaacgg cacgatcaca tcgatgttga acgcgatcaa
      481 cagccccagc gccagccccg ccagcgtacc gatcacgccc accagcgcgc cctgcacgat
      541 gaaggtcttc atgatgctgg ccgggctcgc ccccaacgta cgcaggatgg cgatgtcggc
      601 gcgcttgtcc tgcaccgtca tcaccagcgt gctcaccaaa ttgaacgcgg ccacggccac
      661 gatcagcgtg aggatgatga acatcatgcg tttttcaagc tgcacggcgg caaaccaggt
      721 gcggttctgg cgagtccagt cgaggatcag caaatggccg ctcaaactgc cggccagttg
      781 gtcggccacc tgccgcgcgt ggtgcaggtc gcgcagcttc aggcgaatgc cggtcgggcc
      841 ttcgagcctg aagatgcgcg ccgcatcctc gatgttgagc atggccagcg ccgagtcgta
      901 ttcgtaatgc ccgcttgaga aggtgcccac caccgtcatc tgcttcaggc gcggcaccac
      961 accggcgggc gtgacctggc cactgggcgc gatcagcgtc acctggtcgc ccgcgaccac
     1021 gcccagcttg cgcgccaggt cggcaccgag caggatgccg aactcgcccg gcaccagctt
     1081 cggtaaaagc gtgtctttca attcggccgc aacgtcggtc acgttcggct ccatcgccgg
     1141 atcgatgccg cgcacgatgg cgccctgcat ggtctcgccg cgtgccagca ggccctgcgc
     1201 gccgatgaag ggcgcggcgc cgatcacctc ggggttggcg cgcacctcgt tcaaggtgcg
     1261 ctgcgggtcg ggcagcgcag cgccatcggg cgaaaacacc tcgacgtgcg agatcacgct
     1321 cagcatgcgg ccggccacgt ctttctgaaa gccgttcatc acgctcagca cgatgatcag
     1381 cgccgccacg cccagcgcga tgcccagcat cgacgctgcc gagatgaagc tgatgaagcc
     1441 gttgcgccgc gtggcgcgtc cggcgcgcgt gtagcgccag ccaagcagcc attcgtaggg
     1501 caatcgctgg gacaaggaca tgggtttgga ctgatagccg cctgccgcag ccggcagcgg
     1561 tcaaagggtc gaattctcgc atccccgaca atcgccgtat gccgcccgcg cccgagccgt
     1621 ttcacctgct gattgccgat gccgcgccgc tgttggccga cggcgccgcg ccgccgccgc
     1681 tgcccgccct gccccagctg gatgcgctgc tgaagcgtct gcgtgtgacg gagaccattg
     1741 catgcgacga cgacgcgccc gacacgccgc tggagcgcgc cctggcccgc gcccacggcc
     1801 tgcccggcgc gcccggtcag gtgccgtggg cggcgttcga gaccggcacg gtcggcacgc
     1861 cctgcgcctg gctgcggccc tgccactggc aattgggcat ggaccacatc accttgcttg
     1921 acccagcgct gctcgacttg agcgaagccg aatcgcgcgc cctgctggcc gccgtgcagc
     1981 cgctgctgca agaagacggc atcgccctgc gctacgtgcg gcccgatgcg tggctggccg
     2041 agggcgagct gtttcgcagc ctcgccacct catcgatggc gcgcgccgcg cagcaaccgc
     2101 tcacgcgcga cgcgctggcc gccgcgccca cgcccgcgca aagcgcccac ctgcggaggc
     2161 tgcagaacga gttgcagatg ctgctctaca cccagcccgt caacgacgcg cgcgagcagg
     2221 ccaagcggtg gcccgtcaac gcgctgtgga tcgagggcgc gggcctgctg gacgcgccga
     2281 cgccacaaaa ccccggcgtg cgtgtcgaaa gccgtctggc gccggccgcc acgcccgatc
     2341 tcgctgcgtg gcaagccgcc tggcaggcca tcgatgccga cagcgtggct caactcggcc
     2401 gtcaactggc gtccggcgcc gacgtgcgcc tgaccctgtg cggcccgcgc cgcgcgctga
     2461 ctctgcgccc ggcgaccggc ttgggcgcgc gcgtttcaag ccttttcagc cccttgcgct
     2521 tgtcagacct gcgcaaccag ctatgaattt tgatgtagga gcggcctggg ccgcgatcgg
     2581 gcttgcgccc tgacacggcc ctatcgcgga caagcccgct cccacatgct ccacccaccc
     2641 ccgccatgaa gattgaagtc agagacgttc cgccacgtgc cgcctgggcg ctggaacagg
     2701 ccggcgtaca ccctttgctg gcgcgcctgt tcgctgcgcg cggcgtgcag tcggccgccg
     2761 atctggacga tgcgctggcg cgcctgctgc cgcccgcagg gctgaaaggc gcgcgcgaag
     2821 ccgccgtgct gctggccgac gccatcgccg cgcaaaagcg gctgtgcatc gtcgccgact
     2881 acgactgcga tggcgccacg gcctgcgccg tcggtgtgcg cggcctgcgt ctgctgggcg
     2941 cgccgcacgt cgactacctg gtgcccgacc gcgtggtcga tggctatggc ctcacgccgc
     3001 cgatcgccga gcgcgtggcg acccagggcg cggatgtgct gatcaccgtg gacaacggca
     3061 tcgccagcgt ggatggcgtg gcgcgcgcgc gggcgttggg actggcagtg ctggtgaccg
     3121 accaccacct gccgggcgcc gaattgccgg gcgccgacgt catcgtcaac cccaaccagc
     3181 ccggctgcga attcgagagc aagtccatcg ccggcgtggg cgtcatgttc tacgtgctgc
     3241 tggcgctgcg ggcggagttg cgcgcgcgtg gcgtttttga tgccgccagt caacccaagc
     3301 tcgatgcact gctgccgctg gtcgcgctgg gcaccgtggc cgacgtggtg cggctcgacg
     3361 ccaacaaccg ccgcctggtc gcgcagggcc tcaagcgcat ccgcgccggg cagttgccgg
     3421 tgggcctgta cgctcttttt tccgtagctt cccgcgcaag ccagaaggcg accaccttcg
     3481 atttcggctt tgccctcggc ccgcgcatca acgccgccgg ccgcctggcc gacatgacgc
     3541 tgggcatcga gttgctgctg accgacgacg cggcgcgcgc ggccgagctg gccgccacgc
     3601 tcgacggcat caaccgcgag cggcgcgaaa tcgaaggcgg catgcgcgag caggcgatgc
     3661 agatcgccga ggggctgttc gaccccgatg cggtcccgcc gtcggccttg gccgtgttcg
     3721 accccgactt tcacgaaggc gtggtcggca tcgtcgccgc gcggctgaaa gaccggctgc
     3781 accgcccgac cttcgtcttc gccgccagcc acgcgccggg gcaggcccac gtgctcaagg
     3841 gctcgggccg ctcgattgcc ggctttcacc tgcgcgacgc gctcgacctg gtagccaagc
     3901 gccaccccgg cgtgctggtc aaatttggcg gccacgccat ggcggccggc tgcaccattg
     3961 ccgaagagca cttcgacacc ttcgacagag cgctgcagca ggtcgccgcg gaatggctgg
     4021 acgccgccac gctcacgcgc cggctggaga ccgacggccc gctgccgccc gaataccggc
     4081 gccccgacat ggtcgacacg ctgcacgccg ccgtctgggg ccagggtttt gcgccgccgg
     4141 tgttcagcga agaggtcgag gtcgtcagcc agcgcctggt cggtgaaaag cacctggcgc
     4201 tgaagttgcg ccaccagggc cagcccatcg acggcatctg gttcggccac accgaaccgc
     4261 ttccggcgcg cgcgctgctg gcgtttcgac tggaggccga tgaatggcag ggcgtgcgcc
     4321 gcgtgcggct gctggtcgag ggcgtggcgg gctagcgctt cgttcagatc aggctgaggc
     4381 tcgcaccccg tcttgctccc tctccccctc gtagagggca ggggtgaggg caaggcggtt
     4441 cacctgcgga cttgggcagc cgtcccagag ggagagggag aaaatcctcc tgtctcactc
     4501 caccgtcagc ttgttgtcct tgatcacctt gccccacttg tcgaactcgg ccgtcacgta
     4561 gcgaccggtt tcggcgggcg tcatgtagtg cgcgtagcag ccggcgccga ccaggcgatc
     4621 ctgcacatcc ttttgcgcca gcacggcttt cagttcgctg ctcatgcggt caatcgctgc
     4681 ggcgggcgtg cccttgggcg ccatcatggc gccgatggaa acggcctcga aacccttgaa
     4741 gccctgctcg gccacggtct tgacgtcggg cacgatgtcg acgcgcctgg gcgaaccgac
     4801 ggccagcgcg cgcaacttgc cgctcttgat gtgcggcagc gcggcgacca aatcgctgaa
     4861 catgaccggc acctggccgc ccagcaaatc gttcatggcc ggtgcgctgc ccttgtaggc
     4921 gatgtgctgg gcgtcgaagc caccctggtg cttgagca
//
LOCUS       DAOMVO010000181         4955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4771_length_4955_cov_11.595918, whole genome shotgun sequence.
ACCESSION   DAOMVO010000181 DAOMVO010000000
VERSION     DAOMVO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_4955_cov_11.595918"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..748)
                     /locus_tag="PKV30_09210"
     CDS             complement(50..748)
                     /locus_tag="PKV30_09210"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRL30426.1"
                     /translation="MTRTRIKICGLTREADVDAAVAAGADAIGFVLYPQSPRFVTPER
                     AGELARRLPPMVTPVLLFVNENAMNIEAACACVPSAALQFHGEESAEACSAEAHATGR
                     VFWRAARIPAGDGAGFDLLKFAHTFQAAQAILLDAHVEGYGGGGKTFDWSRLPPNIPA
                     HLVLSGGLTPANVGAGIAALRGRGHSLAVDVSSGVEADGPDGKPLKGIKDADKIHAFV
                     RAVRAADAQFSIAP"
     gene            complement(745..1527)
                     /gene="truA"
                     /locus_tag="PKV30_09215"
     CDS             complement(745..1527)
                     /gene="truA"
                     /locus_tag="PKV30_09215"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRL30427.1"
                     /translation="MRIALGISYLGTHYQGWQSQPSGQTVQDKLERALSDFATVPVAT
                     LCAGRTDAGVHGLMQVVHFDTELQREPFSWVRGTNRFLPPDIAVQWAQAVPDAFHARA
                     SALARRYAYVLLESPVRPSVDAGRVGWSFRPLDGDAMRQAAGRLIGEHDFTSFRASAC
                     QALSPVKQLTRIDVTRRGAYWRVDFEASAFLHHMIRNIMGCLVAIGQGLQPPEWIDAV
                     LAARSRDAAAPTFAPDGLYFLGPRYDAHWGLPDRTAAYDALP"
     gene            complement(1635..4376)
                     /locus_tag="PKV30_09220"
     CDS             complement(1635..4376)
                     /locus_tag="PKV30_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HRL30428.1"
                     /translation="MAKRPWKRGVLASAVALTMLGVSLEAQALALGAITVRSALGEPL
                     RAEIEVPQISSEEAATFKAAVASPQAFRSAGVDYSPALSGARVTLHRRPNGQAYLRVV
                     GDRPINEPFLGIVIDADWGGGRVVRDYTMLVDPPGRVAPPPVAVTQSQVAPPAPVMAP
                     APAVVQAPMAVQTTPAPAATPAPRVRPAPVAEAPRRRAAPTSATSGDGQQVTVQRGDT
                     ASGIASAHAAEGVSLDQMLVAMLRANPGAFIGGNVNRMRAGAVINMPTAEQAAATPRQ
                     TARRTVVAQSRDFNAYRRGVAGRSGTTRVAAATRSTSGGVQPQVQETRPAAPSPDKLT
                     LSRGGTPSGAEAKVAQSRQAQEQSERVAELNKNISELSRLQGASGAAPAAPAASPASA
                     AVPAINVPTGAPAAPAPAPAAATPTPPPVAAAASAAVATAAVAAASAASAATDAASEA
                     VTAAASAVASASEAAPAATQAASAADDAASAAAASAAASAPAAPVVVPAPAPVEEPSF
                     LDSLADNPLVLPGLGLLLAALAGLGLYRARQRKKDAAPLDSSFNESRLQPDSFFGASG
                     GQRVNTKEGASSVSGNSSMAYSPSQLDAAGDVDPVAEADVYLAYGRDMQAEEILKEAL
                     RSHPGRVAIHRKLAEIYAKRRDARAFEAVAVEAYGLTQGQGPEWLAITSLGSELDPGN
                     QLYQPGGSPVARNAPAPVRATFGADTEPQTAQLLDPKGTPSTNAALDLDLDLDEAPVR
                     AGATTAPAPLPPAVPTPAATMAPAVAAGVAAAATSAAAARISVPPPPPSDPELHAPLD
                     FDLDLDFAPPSELPPAAPAPVAAQPVAPAGKADSGMIDFDMNALSVDPDSRSGGDLKT
                     EQPDDADEDPLGTKLSLAQEFHAIGDTEGARTLVKEVIAESSGALRVRAERFLAELN"
     gene            complement(4642..>4955)
                     /locus_tag="PKV30_09225"
     CDS             complement(4642..>4955)
                     /locus_tag="PKV30_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HRL30429.1"
                     /translation="RIGAMRCHSQALTFKLKKDVPVADIEALIASDNEWVKVVPNNRE
                     DTMRDLTPVAVTGTLGIPVGRIRKMAMGPEYVGAFTIGDQLLWGAAEPLRRTLRILLA
                     A"
BASE COUNT          761 a         1608 c         1817 g          769 t
ORIGIN      
        1 ccgccgtagc ggccgaaatg cccgctcgcg tcgggctgct ggtaatcgat catggtgcta
       61 tggaaaattg agcatctgcc gcacgcacgg cgcggacaaa cgcgtgaatc ttgtcggcgt
      121 ccttgatgcc cttcaggggc ttgccgtcag gcccatcggc ctcgacgccg gaggaaacgt
      181 caaccgcgag cgagtgaccc cgcccgcgca gcgcagcgat gccggcgccc acgtttgcag
      241 gtgtgagccc accagacaag acgaggtgag cgggaatgtt tggtggcagg cgtgaccaat
      301 cgaatgtctt tccgccgccg ccatagccct cgacgtgggc atcgagcagg atggcttgcg
      361 cggcctgaaa agtgtgggcg aattttagca agtcgaatcc cgcaccatcg cctgcgggaa
      421 tgcgcgctgc gcgccagaag acgcgccccg tggcatgcgc ctcggcgctg cacgcctcgg
      481 ccgattcttc accatgaaat tgcagcgcag cgcttggtac acaagcgcaa gcagcttcta
      541 tattcatagc attctcgttg acgaacagca gcaccggcgt gaccatgggc ggcagtcggc
      601 gcgctaactc acccgcgcgc tctggggtca cgaagcgcgg gctttggggg tacagcacga
      661 agccgatggc gtcggcaccg gcggccacgg ccgcgtcaac gtcggcttcg cgggtcaggc
      721 cgcagatctt gatccgggtg cgggtcatgg cagcgcatca taggccgccg tgcgatccgg
      781 caagccccag tgcgcgtcgt agcgcggccc gaggaaatac aggccgtcgg gcgcgaaggt
      841 gggcgccgct gcatcgcggc tgcgcgcggc cagcacggca tcgatccact cgggtggctg
      901 caggccctgg ccgatggcga ccaggcagcc catgatgttg cggatcatgt gatgcaaaaa
      961 ggcgctggct tcgaagtcga cgcgccaata ggcgccgcgc cgggtgacat caatgcgggt
     1021 gagttgcttg accggcgaga gcgcctgaca tgcactggcg cgaaacgacg tgaagtcgtg
     1081 ctcgccgatc aagcggccgg cggcctggcg cattgcatcg ccgtcaagcg ggcgaaacga
     1141 ccagccgaca cgcccggcat cgacgctcgg ccgcaccggc gattcaagca gcacgtaggc
     1201 gtagcggcgg gcgagggcgc tggcgcgggc gtggaaggcg tctggcacgg cttgcgccca
     1261 ctgcaccgcg atgtcgggcg gcagaaaacg gttggtgcca cgcacccacg agaagggctc
     1321 gcgctgcagc tcggtatcga aatgcaccac ctgcatcagg ccgtgcacgc cggcgtcggt
     1381 gcggccagcg cacagcgtgg cgacgggcac ggtggcgaag tcggacagtg cccgctcaag
     1441 cttgtcctga acggtctggc ccgacggctg gctttgccag ccctgatagt gggtgccgag
     1501 gtagctgatg ccgagcgcga ttctcacgat ggcctgattg tcgaagttgt ttgtcggcgc
     1561 ggttcaggcg cgccaaatgc cgcgcggcgc ctcgagggcg ccgcgcggaa tggatggttg
     1621 aacgaaaccg cctgtcagtt cagctctgcg aggaagcgct cggcgcgcac gcgcagggca
     1681 ccggaggact cggctatcac ttccttgacc agcgtgcggg cgccttcggt gtcgccgatg
     1741 gcgtggaatt cctgcgccaa cgacagcttg gtgccaagtg gatcttcgtc cgcgtcgtcg
     1801 ggctgctcgg tcttcagatc gccgccagag cgcgagtcgg ggtcgaccga cagcgcattc
     1861 atgtcgaagt cgatcatgcc gctgtcggcc ttgccggcag gcgcgaccgg ttgtgcggcc
     1921 acgggcgcgg gcgcagcggg gggcagctct gacggtggtg caaagtcgag atcgaggtcg
     1981 aagtcgagcg gcgcatgcag ttccggatcg gacggagggg gcggcggcac cgagatgcgc
     2041 gctgcagcgg ccgacgtggc ggcggcagcg acaccggcag ccacggcagg cgccatggtg
     2101 gcggcaggcg taggcaccgc tggcggcagc ggggcgggcg cggtggtggc gcctgcgcgc
     2161 accggcgcct cgtccaggtc gaggtccagg tccagcgctg cgttggtgct tggcgtgccc
     2221 ttggggtcga gcaactgcgc ggtctgcggc tcggtgtcgg cgccgaaggt cgcgcgcacg
     2281 ggggcgggtg cgttgcgtgc caccggcgag ccaccgggct ggtacaactg gttgccgggg
     2341 tcgagttcgc tgcccaggct ggtaatggcc agccattcgg ggccctggcc ctgggtcagg
     2401 ccataggctt cgaccgccac ggcctcgaac gcgcgcgcat cgcggcgctt ggcatagatt
     2461 tccgccagct tgcggtggat ggcgacgcgg cccgggtggc tgcgcagggc ttccttcagg
     2521 atttcctcgg cctgcatgtc gcggccatag gccaggtata catcggcttc ggccacggga
     2581 tcgacgtcgc cggccgcatc cagttggctg ggcgaatagg ccatcgacga attgccgctg
     2641 accgatgacg cgccttcctt ggtgttgacg cgctgaccac cgctggcgcc gaagaacgag
     2701 tcaggctgca agcggctttc gttgaacgaa ctgtcgagcg gcgcggcgtc cttcttgcgc
     2761 tggcgtgccc ggtaaagacc caagcccgcc aaagcggcca acagcaggcc aaggccaggc
     2821 aggacgaggg ggttgtcggc cagcgagtcg aggaacgagg gttcttcaac cggcgccggg
     2881 gctggcacca cgaccggcgc tgccggtgca gacgcagcgg cgctggcggc tgcggcagac
     2941 gccgcatcgt cggcggcgga agcggcttgg gtcgcggcgg gtgccgcctc gctggcactg
     3001 gccaccgcgc tggcggcggc cgtgacggct tcggaagcgg catcggttgc cgcagacgca
     3061 gccgatgcag ccgccaccgc tgcggtggca actgcagcgg atgccgctgc cgccactggc
     3121 ggcggggtcg gggtggctgc tgcaggggcc ggtgcaggcg ctgccggcgc accggtgggc
     3181 acgttgatgg caggcacggc ggcgctggcg ggcgacgcag cgggcgctgc cggcgctgcg
     3241 ccagacgccc cttgcaggcg tgacagctcg ctgatgttct tgttcagttc ggccacgcgc
     3301 tcggattgct cctgcgcttg gcgggattga gccactttgg cttcagcacc gcttggcgtg
     3361 ccgccgcgcg acagcgtcag cttgtccggg ctcggcgcgg cagggcgcgt ttcctgcacc
     3421 tgcggctgca cgccgccgga tgtgctgcgc gtggccgcgg cgacgcgcgt cgtgccactg
     3481 cgtccggcga cgccgcggcg ataggcattg aagtcgcggc tttgggcgac gacggtgcgc
     3541 cgggcggtct ggcgcggcgt ggcagcggcc tgctccgccg tcggcatgtt gatgacggcg
     3601 ccggcgcgca tgcggttgac gttgccgccg atgaaggcgc ccgggttggc gcgcagcatg
     3661 gcgaccagca tctgatcgag cgagacgcct tctgccgcgt gggcggatgc gatgccggac
     3721 gcggtgtcgc cgcgctgaac ggtgacctgc tgaccgtccc cgctggtcgc agacgtgggc
     3781 gcagcgcggc ggcgcggcgc ctcagccacc ggcgcggggc gcacgcgcgg cgccggtgtc
     3841 gccgcgggtg cgggcgtggt ctgcacggcc atcggcgctt gaaccaccgc aggcgcaggt
     3901 gccatgacgg gtgctggcgg cgccacctgc gactgcgtga cggccaccgg cggcggcgca
     3961 acgcggccgg gcgggtcgac cagcatggtg tagtcgcgca ccacgcgacc accgccccag
     4021 tcggcatcga tcacgatgcc aaggaagggc tcattgatcg gacgatcacc caccacgcgc
     4081 agataggcct ggccattggg gcgacgatgc agcgtgacgc gcgcgccact gagcgccggc
     4141 gaatagtcga cgccggccga gcgaaacgcc tgcggcgagg cgacggcggc cttgaaggtg
     4201 gcggcttctt ccgatgaaat ctgcggcacc tcgatctcgg cgcgcagcgg ctcgcccagg
     4261 gccgagcgca cggtgatggc gcccagggcc agcgcctgcg cctccaggct gacgcccagc
     4321 atggtcagcg cgacggccga tgccagcaca ccccttttcc acggccgttt ggccatgccc
     4381 tgatcaatcg accccggaag ggtctgcgcg gtcttgttct gcatgtctgg tgatggccaa
     4441 gcgtggcctg ccggagcgga tatgaaaatc gacattaaca tcaacaaatt aggctgacaa
     4501 gctgagacac tggttaacca tcgtcacaag ttcacacagc atcgaaagtg ctgtcgccat
     4561 gttgtcccgg gacaacgggc ggcgcccggc aggcgaagcc cgttgcactt tcgactgacc
     4621 cagcggtcag cggcacagcc ctcaagccgc cagcagaatc cgcagcgtgc ggcgcagggg
     4681 ctcggccgcg ccccacagca actggtcgcc aatggtgaag gcgcccacgt actccggccc
     4741 catggccatc ttgcggatgc ggccgacagg gatgccgagc gtgccggtca cggccacggg
     4801 ggtcaggtcg cgcatggtgt cttcgcggtt gttgggcacc accttcaccc attcgttgtc
     4861 gcttgcgatc agcgcctcga tgtcggccac cggcacgtct ttcttcagct tgaaggtcag
     4921 cgcctggctg tggcagcgca tggcgccgat gcgca
//
LOCUS       DAOMVO010000182         4941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4792_length_4941_cov_11.473393, whole genome shotgun sequence.
ACCESSION   DAOMVO010000182 DAOMVO010000000
VERSION     DAOMVO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_4941_cov_11.473393"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1581)
                     /locus_tag="PKV30_09230"
     CDS             complement(85..1581)
                     /locus_tag="PKV30_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL30430.1"
                     /translation="MTERLVRRPRMPSTPDPPEPELPPGIEGVRQVDPVELDAALGDS
                     LMGTLRGVQLPVVVVALIFVALLYSIRPGPWPVAWAVATVGIELLWVVVLRHYSRHVL
                     HASPAERVAFVRRLRPLWRLNGALWGASPLIFAGAPVSMNLLVCWLILGCFGAIVSNA
                     MAPHRPSLMAYLNGFAGAMLASYAIDMWLARDLALVNGALLLLMLLYWFLLAYIGRGQ
                     HALLQRAMLLQQRNARLVESLSAHTLALQGALDTRRRFLAVATHDIRQPVHAVRLYAD
                     MLSQDPTQVDELAPRIVKASAAVNGLFENLFDLARIDWNRLHAQPQPVRLGDLLADME
                     VQARPQCADKGLRFRLRTSRALQGATLHTDRLMLQRIVSNLLANAIKYTERGGVLLAL
                     RGTPARPRLEVWDTGIGIPPAAQGRVFDDFFKAHTHFGTQDGFGLGLAIVQQLARQLG
                     CSVALRSQEGRGTLLRVTLPAVEAALPLGAGFKEKTAPALDWQARAAI"
     gene            1695..1835
                     /locus_tag="PKV30_09235"
     CDS             1695..1835
                     /locus_tag="PKV30_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30431.1"
                     /translation="MGEQRREKMVKKPKKDTAPPKTGTPSDRPQAPMTSVLPKGKMKN
                     KQ"
     gene            complement(2006..2775)
                     /locus_tag="PKV30_09240"
                     /pseudo
     CDS             complement(2006..2775)
                     /locus_tag="PKV30_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
     gene            2966..4162
                     /locus_tag="PKV30_09245"
     CDS             2966..4162
                     /locus_tag="PKV30_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30432.1"
                     /translation="MPTSVHEIAIAAALAGGLLALTALALAEWLTEGRGVGALRNLVF
                     VALMGTAVVLIIGLPEALWPALESRVLRMLRASLGPLAASTTLYYLGQWAGGMRQDPV
                     MNRITNWSAAVMLVVALGLALAGLLVPPERFRDVFMLTAISNVLAVLPGFAAAFRAAK
                     LGDRLARGMAGATTVLAAVTTGLYSLGLSRHDLGAGAWALTAVLTLTFLILTSTLVSV
                     RNRELRRLGRLSRIEPGVEPATQLPTGSHLLRKVEDAFWTAGRRQAQCAVVCIYLDNL
                     YELDAENRSAEYQLLAAMAARIRRAAGFRGVVGIDHPRCFMAVLALDADRQPFAAMLQ
                     RLRAYVGESVDLVGPDRAPQRFTPRVGIGVVVADAVGAVPRVLLDEAEAAARAAVPPQ
                     LMDTQY"
     gene            complement(4178..4750)
                     /locus_tag="PKV30_09250"
     CDS             complement(4178..4750)
                     /locus_tag="PKV30_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30433.1"
                     /translation="MATDPKKTAPPEEPPTAAPAGIPSQDPRLKAQEPLTPSERASEA
                     NSVVMGGGMMAGAAAGAAAGAAIGGPVGVIVGGTAGAIVGALGGYAAVSAGDPQYDYW
                     RDNHVGQPGYVDGYNYEDDYAPAYRLGYQGRERYAGRAWDQTEADLQRDWESVKGKSR
                     LTWEQARAATRNAWHRVEQALPGDADGDGR"
BASE COUNT          779 a         1699 c         1742 g          721 t
ORIGIN      
        1 gtgggcatcg acctgcgttc ggccggcttt ggcgtctggc aggcgtgagc ggcacgcgca
       61 acgccatgcg cggatcgcta ttctttaaat agctgctcgc gcttgccaat caagcgctgg
      121 cgccgttttt tccttgaaac cggcacccaa cggcagcgcc gcctcaaccg ccggcagcgt
      181 cacgcgcagc agcgtgccgc gcccttcctg cgagcgcaag gcaacgctgc aacccagctg
      241 ccgcgccagt tgctgcacga tggccaggcc caggccgaag ccgtcttgcg tgccgaagtg
      301 cgtgtgggcc ttgaagaagt cgtcgaacac ccggccctgc gccgccggcg gaatgccgat
      361 gccggtgtcc cacacttcca gccgcggccg cgccggcgtg ccgcgcagcg ccagcagcac
      421 gccgccgcgc tcggtgtact tgatggcgtt ggccagcagg ttgctgacga tgcgctgcag
      481 catcagccgg tcggtgtgca gcgtggcgcc ctgcagcgcg cggctggtgc gcaggcgaaa
      541 gcgcaggccc ttgtcggcgc actgcgggcg ggcctgcact tccatgtcgg ccagcagatc
      601 gcccaggcgc accggctgcg gctgcgcgtg caggcggttc cagtcgatgc gggccaggtc
      661 gaacaggttc tcgaacaacc cgttgacggc cgcgctggcc ttgacgatgc gcggcgccag
      721 ctcgtccacc tgcgtcgggt cttgcgacag catgtcggcg tacagccgca ccgcgtgcac
      781 cggctggcga atgtcgtgcg tggccacggc cagaaagcgc cggcgcgtgt cgagcgcgcc
      841 ctgcagcgcc agcgtgtgtg cgctgagtga ttcgaccagc ctggcgttgc gctgctgcag
      901 cagcatggcg cgctgcagca gcgcgtgctg accgcgcccg atgtaagcca gcaagaacca
      961 gtacagcagc atgagcagca gcagcgcgcc gttgaccagc gccagatcgc gcgccaacca
     1021 catatcgatg gcgtaggaag ccagcatggc cccggcaaag ccgttcaggt aggccatcag
     1081 gctgggccgg tgcggcgcca tggcgttgct gacaatggcg ccaaagcagc ccagaatgag
     1141 ccagcacacc agcaggttca tcgataccgg cgcgccggca aaaatcagcg gcgacgcgcc
     1201 ccacagcgcg ccgttgagcc gccacagcgg gcgcagccgg cgcacgaagg ccacgcgctc
     1261 ggccgggctg gcgtgcagca cgtggcggct gtagtggcgc agcaccacga cccacagcag
     1321 ctcgatgccg accgtggcca ccgcccacgc cacgggccac ggcccgggcc gaatcgaata
     1381 cagcagcgcc acgaagatca gcgccaccac caccaccggc agctgcacgc cgcgcagcgt
     1441 gcccatcagg ctgtcgccca gggcagcgtc cagctcgacc ggatcgacct ggcgcacgcc
     1501 ctcgatgccc ggcggcagct cgggctcggg tggatcgggg gtggaaggca tgcgggggcg
     1561 gcgcacgagg cgctcggtca ttctacggac ggtatgccga ccgccccgca ttttcggatg
     1621 tgcaattcgc catcgacagc cgcgcgggcc ggctaccatc gccgcaccac aacaaccgca
     1681 accaaggagc cgacatgggt gaacagcgcc gagagaagat ggtgaaaaag ccgaagaagg
     1741 acaccgcgcc gcccaagacg ggcacgccct cggaccgccc gcaggcgccg atgacgtcgg
     1801 tgctgcccaa gggcaagatg aagaacaagc agtgaatctg cttttgtttt gatagctgct
     1861 ggcgctggat ggacaagcgc tggcggcact ttttatctaa atcacaaaca acccaacccc
     1921 gttcgtcctg agcctgtcga aggacttggc gaacggatga cggctgcggc gcgcgtcagg
     1981 tcagcacata gtcgtgcggc tggggctatg gcggccagtc gtgctcgccc atcgcttcga
     2041 gcagcgaggt ctcgatggtc cagcacagcg catccagcgg caggtcgttg gcttgcgaac
     2101 cgaagggctc ttccagctcg gagcccagcg cttccagcgc caggcaggtg taggccacga
     2161 agaccacgat caccggcgtc atgctgccga tcgaatcgac cagcccaaac ggcagcagca
     2221 ggcaatacag ataaatcgtg cggtggatga tgacgccgta ggtgaacggg atcggcgtgg
     2281 cggccagccg ctcgcagccg ccgacggcgt cgccgatgtg cgtgaattgc gcttccagcg
     2341 ccggcaccag cgcgggggcg atgcggccct ggcggcggca ccgccgcgca gttggtggcg
     2401 cagctcatgc ggcagcgcga tcaggcggcg cgccagcacg gtggcatcgc cctgcccgcc
     2461 atcgaccagc gtcagcgcct ggcgcaccgc cgcgcggctg gcgatgagca actggcccca
     2521 cagcacgcgc gcttcccacc agcggctgta gctggtgccg ttgcgaaagc cgagaaagat
     2581 cgccagcgtg aggccgatga gcgaaaacgg cacgaagttg agcggcacct tccagcgcca
     2641 gacctggccg tgcaccagcg tcaccagcac cgcaaaaccc agcgccgcca gcagctgcgg
     2701 caggatctgc ggcaggatcg agccgcgcca gacaaacagc aggcgcagcc agttggggcg
     2761 gtcgcggacg atcatgcgtg tgaatgaagc cagggcgtga aagcgcccga tgtgacgggc
     2821 tgtggcacgc cgccgtcatg ggtcagttcc aacgagatac accacccgct gcggcgcgcc
     2881 ggcgctgcat gtcggcgtga agtaatgcac agcgcgcgca ctgatcgtgg cgggaaagca
     2941 tcgggcaacc cccaaaatcg cgcgcatgcc gacatcggtg cacgaaatcg ccattgccgc
     3001 cgccctggcc ggcggcctgc tcgcgctgac cgcgctggcg ctggccgagt ggctgaccga
     3061 aggtcgcggc gttggcgcgc tgcgcaacct ggtgttcgtg gcgctgatgg gcacggcggt
     3121 ggtcttgatc atcggcctgc ccgaagcgct gtggccggcg cttgaaagcc gcgtgctgcg
     3181 catgctgcgc gccagcctgg gcccgctggc ggcgtcaacc acgctgtact acctgggcca
     3241 gtgggccggc ggcatgcggc aagacccggt gatgaaccgc atcaccaact ggagcgccgc
     3301 cgtcatgctg gtggtcgcgc tgggcctggc gctggccggc ttgctggtgc cgcccgagcg
     3361 ctttcgcgat gtcttcatgc tgaccgccat cagtaacgtg ctggcggtgc tgccgggctt
     3421 tgcggcggcg tttcgcgccg ccaagctggg cgaccggctg gcgcgcggca tggcgggcgc
     3481 gaccacggtg ctggccgccg tgaccaccgg cttgtattcg ctcggcctca gccggcacga
     3541 tctgggcgcg ggcgcgtggg cactgaccgc ggtgctcacg ctcacgttct taatcctgac
     3601 atcgaccctg gtcagcgtgc gcaaccgcga gctgcgccgg ctcggccgcc tgtcgcgcat
     3661 cgaacccggc gtggagccgg ccacgcaact gcccaccggc tcgcacctgc tgcgcaaggt
     3721 ggaagacgct ttctggaccg ccgggcggcg ccaggcgcag tgcgcggtgg tgtgcatcta
     3781 cctcgacaac ctgtacgagc tggacgccga aaaccgcagc gccgaatacc agctgctggc
     3841 cgcgatggcg gcgcgaatcc ggcgcgcggc gggctttcgc ggcgtggtgg gcatcgacca
     3901 cccgcgctgc ttcatggcgg tgctggcgct ggacgccgac cggcagccct tcgcggccat
     3961 gctgcagcgc ctgcgcgcct acgtgggcga gtcggtcgat ctggtggggc cagacagggc
     4021 gccgcaacgc ttcacgccgc gcgtcggcat cggtgtggtg gtggccgatg cggtgggcgc
     4081 ggtgccgcgc gtgctgctgg acgaggctga agcggcggcg cgcgccgccg tgccgccgca
     4141 gctcatggac acgcagtatt gagcgcggcc gagcggctca gcgcccgtcg ccgtccgcat
     4201 cgcccggcag cgcttgttcg acgcggtgcc aagcgttgcg ggtggccgcg cgggcctgct
     4261 cccaggtcag gcgcgacttg cccttgacgc tttcccagtc gcgctgcagg tcggcctcgg
     4321 tctggtccca ggcgcggccg gcgtagcgct cgcggccctg gtagccgagg cggtaggccg
     4381 gcgcgtagtc gtcttcgtag ttgtagccgt ccacgtagcc cggctggcca acgtggttgt
     4441 cgcgccagta gtcgtattgc ggatcgcccg cgctgacggc cgcgtagccg cccagcgcac
     4501 cgacgatggc gccggccgtg ccgcccacga tgacgccgac cggcccgcca atcgcggcgc
     4561 cggcggccgc accggcggcg gcgccggcca tcatgccgcc acccatgacg accgagttgg
     4621 cctcggacgc gcgttcagac ggcgtgagcg gctcttgcgc cttcaggcgc gggtcttgcg
     4681 acggaatgcc ggcgggcgcg gcggtgggcg gctcttcggg cggcgcggtt ttcttggggt
     4741 cggtggccat ggtcggctcc ttgaaatgtt ggggaacatg ccagcgtgcc ggcatgagcc
     4801 gactgtgcgc cgccccgcgc gccgccgctg tcgcccaagg gccgcatcgg gtgtcggcca
     4861 gacctgacac agcgtcgatg cgggtgcggc atcatgtcac tctatttttg atagctgctc
     4921 gcgctggatg gacaagcgcc a
//
LOCUS       DAOMVO010000183         4915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4828_length_4915_cov_12.975309, whole genome shotgun sequence.
ACCESSION   DAOMVO010000183 DAOMVO010000000
VERSION     DAOMVO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_4915_cov_12.975309"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKV30_09255"
     CDS             complement(<1..883)
                     /locus_tag="PKV30_09255"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRL30434.1"
                     /translation="MSRLPPVLQRVKFPVIGSPLFIISNPKLVIAQCTSGVVGSMPAL
                     NARPAEQLDEWLAEITEALAAWDKAHPDEPAAPFAINQIVHKSNDRLEQDMQLCAKYK
                     VPVIITSLGAREDVNQAVHGWGGVVLHDVINNVFAHKAIEKGADGLIPVAAGAGGHAS
                     AKSPFAMVQEIREWFDGPVALSGAISSGGAILAAQACGADFAYIGSAFIATDEARAVD
                     GYKQMIVASDSDDIVYSNFYTGILGNYLKGSIRNAGMDPENLPQSDPSKMNFGTVGGD
                     ARAAKAWKDIWGCGQGIG"
     gene            complement(981..2768)
                     /locus_tag="PKV30_09260"
     CDS             complement(981..2768)
                     /locus_tag="PKV30_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRL30435.1"
                     /translation="MSYKAPVKDMLFNIEHLARIDQIAQMPGFEDAGLETAQAVLEEC
                     ARFNEEVVAPLNFDADKAPSTHSGTSVTTSKGFKDAYKQYTEGGWQGLQHPSDFGGQG
                     LPKTIGAACVEMLNSANLSFALCPLLTDGATEALLTAGSDELKSTYLEKLVSGDWTGT
                     MNLTEPQAGSDLAQVRTRAEPQPDGTYKVFGTKIFITYGEHDMADNIVHLVLARVQGA
                     PEGVKGISLFVVPKFMVNQDGSLGERNDVYCVSIEHKMGIKASPTAVLQYGDHGGAIG
                     YLVGEENRGLEYMFIMMNAARYAVGVQGIAIADRAYQKAVQYANDRVQSRPVDGSVKG
                     PATIIHHPDVRRMLMTMRALTEGCRAMASTAAAAYDAAHHHPDAEVRQQNQTFYEFMV
                     PLVKGYSTEMSLEVTDLGVQVHGGMGFIEETGAAQYYRDAKILTIYEGTTAIQANDLV
                     GRKTARDGGLTAKAIAAQIEATEKELAAGSDDAKAVAKRLKAAREAFVDVVDFIAAQS
                     KADPNAAYAGSVPYLLLAGNLVAGWQLGRALLVAEKQLAAGHDTAFMKAKVATARFYA
                     DHILTRVPGTRDAIVDGAGSVTALALESF"
     gene            complement(2861..3793)
                     /locus_tag="PKV30_09265"
     CDS             complement(2861..3793)
                     /locus_tag="PKV30_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRL30436.1"
                     /translation="MTALVIAEHDNASIKPATLNTVTAAAACGGDVHVLVAGHNAAEA
                     AKAAGQIAGVSKVIHADSDALAHGLAENVAAQVLALAGNYSHILFPATASGKNVAPRV
                     AAKLDVAQISDISKVDSSDTFERPIYAGNAIAIVQSSDPVKVITVRTTGFDAAAATGG
                     SAAVESVSATADSGKSSFVGQEIAKSDRPELTAAKVVVSGGRALGSSEKFTEVMTPLA
                     DKLGAAIGASRAAVDAGYAPNDLQVGQTGKIVAPQLYVAAGISGAIQHLAGMKDSKVI
                     VAINKDEEAPIFSVADYGLVADLFTAVPELTQAL"
     gene            complement(3808..4557)
                     /locus_tag="PKV30_09270"
     CDS             complement(3808..4557)
                     /locus_tag="PKV30_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRL30437.1"
                     /translation="MKALVAVKRVVDYNVKVRVKSDQTGVDTANVKMSMNPFDEIAVE
                     EAVRLKEKGVVTEVIAVSCGDAKAQETLRTAMAIGADRGILVETSEELQPLAVAKLLK
                     ALVDKEQPQLIILGKQAIDDDANQTGQMLAALADLPQATFASKVEIEGDKAKVTREVD
                     GGLETIAISLPAVVTTDLRLNEPRYVTLPNIMKAKKKQLDVVKPEDLGVDVKPRLKTL
                     KVTEPPKRGAGIKVPDVATLVDKLKNEAKVI"
     gene            complement(4727..>4915)
                     /locus_tag="PKV30_09275"
     CDS             complement(4727..>4915)
                     /locus_tag="PKV30_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352100.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRL30438.1"
                     /translation="AKPREAIAAGKELFYRQSELGIEAAYQLAGQTMACNMMLDVAQE
                     GVQAFIEKRAPAWRQTLA"
BASE COUNT          720 a         1581 c         1617 g          997 t
ORIGIN      
        1 cgccaatgcc ctggccgcag ccccagatgt ccttccacgc cttggccgcg cgcgcatccc
       61 cacccacggt gccgaagttc atcttgctcg gatcgctttg cggcaggttc tccgggtcca
      121 tgccggcgtt gcggatgctg cccttcaggt agttacccag gatgccggtg tagaagttgc
      181 tgtagacgat gtcgtccgaa tcgctggcca cgatcatctg cttgtagcca tccaccgcgc
      241 gcgcctcgtc ggtggcgatg aaggccgagc cgatgtaggc gaagtcggct ccacaagcct
      301 gcgccgccag gatggcccca cccgatgaga tggcgcccga cagcgccacg gggccgtcga
      361 accattcacg gatttcctgc accatggcaa acgggctctt ggcgctggcg tgaccgccgg
      421 cgccggccgc cacgggaatc aggccgtcgg cgcccttctc gatcgccttg tgcgcaaaca
      481 cgttgttgat gacgtcgtgc agcaccacgc cgccccagcc gtgcacggct tggttcacgt
      541 cttcgcgtgc gcccagcgac gtgatgatga cgggcacctt gtacttggcg cacagttgca
      601 tgtcttgctc cagccggtcg ttgctcttgt gcacgatctg gttgatggcg aacggcgcgg
      661 ccggctcgtc ggggtgcgcc ttgtcccacg cagccagcgc ctcggtgatc tcggccagcc
      721 attcgtcgag ctgctccgcc ggacgcgcgt tgagcgccgg catggaaccc accacgcccg
      781 acgtgcactg ggcaatcacc agcttggggt tgctgatgat gaacaacggc gaaccgatga
      841 cgggaaactt cacacgctgc agaacggggg gcaatctgga catggggatg ctctctcgtg
      901 cggacttcaa gttatgaaca aaaaaggccg ccagcgcttg tccgataagc gctagcagct
      961 atattaaaaa tagcaaacga tcagaaggat tccagcgcca gcgccgtcac gctgcccgcg
     1021 ccatcgacga tggcatcgcg cgtgcccggc acgcgagtca ggatgtggtc ggcatagaag
     1081 cgcgcggtgg cgaccttggc tttcatgaag gcggtgtcgt ggccggcggc cagttgcttc
     1141 tcggccacca acagcgcgcg gcccagttgc cagccggcca ccaggttgcc ggcgagcagc
     1201 aggtagggca cgctgccagc gtaggcagcg ttggggtcgg ccttggattg cgcggcgatg
     1261 aagtccacca cgtcgacaaa ggcttcgcgc gcggctttca ggcgcttggc gacggccttg
     1321 gcgtcatcgc tgccggcagc caattccttc tcggtggcct cgatctgcgc cgcaatcgcc
     1381 ttggccgtca ggccgccgtc gcgcgcagtc ttgcggccca ccaaatcgtt ggcctgaatg
     1441 gcggtggtgc cttcgtagat ggtcaggatc ttggcgtcgc ggtagtactg cgcggccccg
     1501 gtctcctcga tgaagcccat gccgccgtgc acctgcacgc ccaggtcggt gacttccagg
     1561 ctcatctcgg tgctgtagcc cttgaccagc ggcaccatga attcgtagaa ggtctggttc
     1621 tgctggcgca cctcggcgtc ggggtggtgg tgcgcggcgt cgtaggcggc ggcggccgtg
     1681 ctggccatgg cgcggcagcc ttcggtcagc gcgcgcatcg tcatcagcat gcggcgcacg
     1741 tcggggtggt gaatgatggt ggccgggcct ttcacgctgc catcgaccgg gcggctttgc
     1801 acgcggtcgt tggcgtattg cacggctttt tgataggccc gatcggcaat cgcgatgccc
     1861 tgcacgccga cggcgtagcg cgcggcgttc atcatgatga acatgtattc gaggccgcgg
     1921 ttttcctcgc ccaccaagta gccgatggcg ccaccgtggt cgccgtactg cagcacggcg
     1981 gtgggcgatg ccttgatgcc catcttgtgc tcgatgctga cgcagtacac gtcgttgcgc
     2041 tcgcccagcg agccatcctg gttcaccatg aatttcggca ccacgaacag gctgatgccc
     2101 ttgacgccct cgggcgcgcc ctgcacgcgc gccagcacca ggtggacgat gttgtccgcc
     2161 atgtcgtgct cgccgtaggt gatgaagatc ttggtgccga ataccttgta cgtgccatcg
     2221 ggctgcggct cggcgcgcgt gcgcacctgc gccagatcgc tgccggcctg cggctcggtc
     2281 aggttcatgg tgccggtcca gtcgcccgac accagttttt cgaggtaggt ggacttgagc
     2341 tcgtcactgc cggccgtgag cagcgcctcg gtcgcgccat cggtcagcag cgggcacagc
     2401 gcaaagctca ggttggccga gttgagcatc tcgacgcagg cggcgccaat ggttttcggc
     2461 aagccctgac cgccgaaatc cgacgggtgc tgcaggcctt gccagccgcc ttcggtgtac
     2521 tgcttgtacg cgtccttgaa gcctttgctg gtggtgaccg acgtgcccga gtgggtggaa
     2581 ggcgccttgt cggcgtcgaa gttgagtggc gccaccacct cttcgttgaa gcgcgcgcat
     2641 tcttccagca ccgcctgcgc cgtctccagg cccgcgtcct cgaagccggg catctgcgcg
     2701 atctggtcga tgcgcgccag gtgctcgatg ttgaacagca tgtccttgac gggggccttg
     2761 tagctcatgg cttttctccg gtgattcttg tgacaaaaaa atgggcatcg cgcgcgatgc
     2821 ccttgcctga cgcgctctcg aaatccgagc gcagggcgac tcaaagggct tgggtcagtt
     2881 ccggcacggc cgtgaacaga tcggccacca ggccgtagtc ggccacgctg aaaatcggcg
     2941 cctcttcgtc cttgttgatg gcaacgatga ccttgctgtc cttcatgccg gccaggtgct
     3001 ggatggcgcc cgagatgccg gcagccacgt acagctgcgg cgccacgatc ttgccggttt
     3061 ggccgacctg caggtcgttg ggcgcgtagc ccgcatccac ggcggcgcgg ctggcgccga
     3121 tggcggcgcc cagcttgtcg gccagcgggg tcatgacttc ggtgaacttc tcgctgctgc
     3181 ccagcgcgcg cccgcccgaa accacgacct tggccgccgt cagctcgggc cggtcgctct
     3241 tggcgatctc ctggccgacg aagctgctct tgccgctgtc ggccgtggcg ctgacgcttt
     3301 ccaccgccgc gctgccgccc gtggctgcag cggcatcgaa gccggtggtg cgcaccgtga
     3361 tcaccttcac cggatcgctg ctttgcacga tggcgatggc gttgccggcg tagatggggc
     3421 gctcgaaggt gtcggacgaa tcgaccttgc tgatgtcgct gatctgcgcc acgtcgagct
     3481 tggcggccac gcgcggggcc acgttcttgc cgctggcggt ggccggaaac aggatgtggc
     3541 tgtagttgcc agccagtgcc agcacctgcg cggcgacatt ctcggccaag ccgtgggcca
     3601 gcgcatcgct gtcggcgtga atcactttgc tgacaccggc gatctggcca gcggccttgg
     3661 cggcctcggc ggcgttgtgg ccggcgacca gaacgtgcac gtcgcccccg caagctgcgg
     3721 cggcggtgac ggtgttgagg gtggccggct tgatgctggc gttgtcgtgt tcggcaatga
     3781 cgagtgcggt catgttcttg tctttcctca gatcactttg gcttcgttct tgagcttgtc
     3841 caccagcgtg gcgacgtcgg gcaccttgat gccggcgccg cgcttgggcg gctcggtcac
     3901 tttcagcgtc ttcagccgcg gcttgacgtc cacccccagg tcttcgggct tgaccacgtc
     3961 aagctgcttt ttcttggcct tcatgatgtt gggcagcgtg acgtagcggg gctcgttcag
     4021 gcgcaggtcg gtggtgacca cggcgggcag gctgatggcg atggtttcca ggccgccgtc
     4081 gacttcgcgc gtgaccttgg ccttgtcgcc ttcgatctcg accttgctgg caaaggtggc
     4141 ctgcggcaga tcggccagcg ctgccagcat ctggccggtc tggttggcgt cgtcgtcgat
     4201 cgcctgcttg ccgaggatga tgagttgcgg ctgctcttta tcgaccagcg ccttcagcag
     4261 cttggcgacg gccagcggct gcagttcttc gctggtttcg accagaatgc cgcggtcggc
     4321 gccgatggcc atggcggtgc gcagggtttc ctgcgccttg gcgtcgccgc aggagacggc
     4381 gatgacctcg gtgacgacgc ccttttcctt caggcgcacg gcttcttcga cggcgatctc
     4441 gtcgaacggg ttcatgctca tcttgacgtt ggcggtgtcc acgccggtct ggtcggactt
     4501 cacgcgcact ttcacgttgt agtccaccac gcgcttgacg gcgacgagtg ctttcatttc
     4561 gggaatctcc tgattgcttt gttgaatgac ggggcccacg cgcgtgcggg cagcaaaaac
     4621 attctagttc agcgtgccgc cctcagcggc tggcgccata gcccaagcga catgcgcaaa
     4681 aaaccgcacg accgtgcttt tattatagat agagcggccc gcgcaatcag gccaatgtct
     4741 gtcgccacgc cggcgcgcgc ttttcgatga aggcctgcac gccttcctgc gccacgtcca
     4801 gcatcatgtt gcaggccatg gtctggccag ccagctggta ggcggcctcg atgccgagtt
     4861 cgctctggcg gtagaacagt tccttgccgg cggcaatcgc ctcgcgcggc ttggc
//
LOCUS       DAOMVO010000184         4907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4846_length_4907_cov_15.457543, whole genome shotgun sequence.
ACCESSION   DAOMVO010000184 DAOMVO010000000
VERSION     DAOMVO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4846_length_4907_cov_15.457543"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /gene="rplE"
                     /locus_tag="PKV30_09280"
     CDS             complement(<1..505)
                     /gene="rplE"
                     /locus_tag="PKV30_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRL30439.1"
                     /translation="MARLQQHYREKVAPELMKKFGYKSPMEVPRITKITLNMGVSEAV
                     ADKKVMDNAVADLTKIAGQKPVVTKAKKAIAGFKIREQQAIGTMVTLRGARMYEFLDR
                     FVTVALPRVRDFRGISGRAFDGRGNYNVGVKEQIIFPEIEYDKVDALRGLNISITTTA
                     KNDEEAKA"
     gene            complement(514..831)
                     /gene="rplX"
                     /locus_tag="PKV30_09285"
     CDS             complement(514..831)
                     /gene="rplX"
                     /locus_tag="PKV30_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRL30440.1"
                     /translation="MNKIRKGDEVIVLTGRDKGKRGTVSLRVCEERVVVDGINVVKKH
                     AKPNPMKGVTGGIVEKSMPIHQSNVAIYNKASGKADRVGVKTLQDGTRVRVFKSSGEE
                     IKA"
     gene            complement(849..1217)
                     /gene="rplN"
                     /locus_tag="PKV30_09290"
     CDS             complement(849..1217)
                     /gene="rplN"
                     /locus_tag="PKV30_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRL30441.1"
                     /translation="MIQTESRLEVADNTGAKSVLCIKVLGGSKRRYASVGDIIKVSIK
                     EAAPRGRVKKGEIYSAVVVRTAKGIRRGDGSLVKFDGNAAVLLNNKLEPIGTRIFGPV
                     TRELRTEKFMKIVSLAPEVL"
     gene            complement(1378..2985)
                     /locus_tag="PKV30_09295"
     CDS             complement(1378..2985)
                     /locus_tag="PKV30_09295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRL30442.1"
                     /translation="MSPVPLPTDRAALLERLAEPRKYDIAVVGGGATGLGVALDAAQR
                     GHSVVLIEAHDFAKGTSSRATKLVHGGVRYLAQGNIPLVREALHERAHILHSAPHLAQ
                     PLPFVMPSYKLWETPFYGVGLKMYDALAGSAGLGRTEWLNAREALQLLPGAQSRGLKG
                     GVKYWDGQFDDARLALAIARTAAAHGALMVNYCEAFEVLHEAGRAAGLRVRDAESGRE
                     FRIQAGCIVNATGVWVDALRQQDAAAAGQPAAPIVAPSQGVHVVVDASFSPGDHALLV
                     PSTGDGRVLFAVPWLGKLILGTTDTPRRDLPLEPAPLAHEVDFILREAAKYLQRAPTR
                     ADIRSTWVGLRPLVKAGGEGGDTKAISREHTVLVAKSGLVTVTGGKWTTYRAMAADVL
                     ARCTQAGVLLPKGTSQTANLKLVGAPGTAAGPLVRLSQPPGAHLYGSEADVVAGLPGA
                     DHALAPGLTEAMVRFAARHEYARTVEDVLARRSRLLFLDAAEAARAAPAAAAVLSSEL
                     GTGHDTALPAFQALAESYIQHGLSAPA"
     gene            complement(3212..4750)
                     /gene="glpK"
                     /locus_tag="PKV30_09300"
     CDS             complement(3212..4750)
                     /gene="glpK"
                     /locus_tag="PKV30_09300"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRL30443.1"
                     /translation="MRYILALDQGTTSSRAIAFDTQGRVAALAQREFAQHFPQPGWVE
                     HDAAEIWATQLAVARECVQKLPPAQSGQAQTAHLIIAISITNQRETTVLWDRATGEPV
                     APAIVWQDRRTTARCEVLRSQGKAPLIQRKTGLVLDAYFSATKLEWLLDQTPGARARA
                     EAGELAFGTVDSWLIWQLTQGRVHATDASNAARTMLMNIHTLAWDDELLRLFHIPRSV
                     LPRIVPSSGVLGDTAPGVLGDAPIPIAGVAGDQQAATFGQACFAPGMAKNTYGTGCFM
                     LMNTGSVAVKSRNQLLTTVGWQGPEQASAPRTAYCLEGSVFTAGATVQWLRDGLQMIQ
                     SASEIEALAASVASAGDVFLVPAFAGLGAPDWDGRARGTLLGLTRGTTRAHIARAALE
                     AIALQSADLFGAMVSDSGIQLTELRVDGGASRNDLLMQMQADLLGVPVVRPQVTETTA
                     LGAAYLAGLATGVWAGGDEIAAQWQVDRRFEPRLPEPERLARIARWREAVARSRGWAK
                     NDPP"
BASE COUNT          719 a         1686 c         1646 g          856 t
ORIGIN      
        1 gcgccttggc ttcctcgtcg ttcttggccg tcgtcgtgat gctgatgttc aggccacgca
       61 gggcgtcgac cttgtcgtac tcgatctcgg ggaagatgat ctgttctttg acgccgacgt
      121 tgtaattgcc gcggccatcg aacgcacgac ccgagatccc gcggaagtca cgcacacgcg
      181 gcaaggccac ggtgacaaag cgatccagga attcgtacat gcgcgcgcca cgcagcgtga
      241 ccatcgtgcc gatggcctgc tgttcacgga tcttgaaacc ggcgatggct ttcttggcct
      301 tggtgaccac gggcttctgg ccggcgatct tggtcaggtc ggccacggcg ttgtccatga
      361 ccttcttgtc ggccacggcc tcgctcacac ccatgttgag cgtgatcttg gtgatgcgcg
      421 gcacctccat gggcgacttg tagccgaact tcttcatcag ctcgggcgcc actttttcgc
      481 gatagtgttg ttgcagtcgt gccatgatgg tcctcaggcc ttgatctctt cgccgctgga
      541 cttgaacacg cgcacgcgcg tgccgtcctg cagggtcttg acgccgacgc ggtcggcttt
      601 gccactggcc ttgttgtaga tggccacgtt ggactggtga atgggcatcg acttttcgac
      661 gatgccgccg gtgacgccct tcatggggtt cggcttggcg tgctttttca ccacgttgat
      721 gccatcgacc accacgcgct cttcgcacac gcgcagcgac acggtgccgc gcttgccttt
      781 gtcacggccg gtcagcacga tgacttcgtc gcctttgcga atcttgttca tgatgcgtgt
      841 tccttcgatc agagcacttc gggcgccagc gacacgatct tcatgaactt ctcggtgcgc
      901 agctcgcgcg tgaccgggcc gaagatgcgg gtgccaatgg gctccagctt gttgttgagc
      961 agcacggcgg cgttgccgtc gaacttgacg agcgagccgt cgccacggcg aatgcccttg
     1021 gcggtgcgca ccaccacggc gctgtagatc tcgcctttct tgacgcgacc acgcggggcg
     1081 gcttccttga tgctcacctt gatgatgtcg ccgacgctgg cgtagcggcg cttggagccg
     1141 cccagcacct tgatgcacag gacggacttg gcgccggtgt tgtcggccac ctcgagtcga
     1201 gattcagtct ggatcatttc ttggtttccc aacttgtacc cagtcgcgtg cgcaaaccgg
     1261 atcagtcttg ggcccgccgc tgttgcactt cgaatgtcaa agcacaccag cgattgggca
     1321 gatttcttgg ccctttttgg gcgaagctct cgattctggc aaaacgccag ccgcttgtca
     1381 agcgggtgca gacaagccgt gctgaatgta gctttcagcc agcgcctgga aggcgggcag
     1441 tgccgtgtcg tgccctgtcc ccagctcact ggacagcacg gcggccgcgg cgggtgcggc
     1501 gcgggcggct tcggcggcgt ctagaaacaa caggcgcgag cggcgcgcca gcacgtcttc
     1561 gacggtgcgg gcgtattcgt ggcgggcggc aaagcgcacc atggcctcgg tcagaccggg
     1621 ggccagcgcg tgatcggcgc caggcaaacc ggccaccaca tccgcttcgc tgccgtacag
     1681 gtgggcgccg ggcggctggc tcaggcgcac cagcgggccg gccgccgtgc cgggcgcgcc
     1741 caccagcttg agattcgccg tttgagatgt gcctttgggc agcagcaccc cggcctgcgt
     1801 gcagcgcgcc agcacgtcgg ccgccatggc gcggtaggtg gtccatttgc cgccagtgac
     1861 agtaaccagg cctgacttgg ccaccagcac cgtgtgttcg cggctgatcg ccttggtgtc
     1921 gccaccttcg ccgcccgcct tgaccagcgg gcgcaggccg acccaggtgc tgcggatgtc
     1981 ggcacgcgtc ggtgcgcgtt gcaggtactt ggccgcttcg cgcaggatga aatcgacctc
     2041 gtgcgccagc ggcgccggct ccagcggcag gtcgcggcgc ggcgtatcgg tggtgcccag
     2101 aatcagcttg cccagccacg gcacggcaaa cagcacgcgg ccatcgccgg tgctgggcac
     2161 cagcagcgcg tggtcaccgg gcgaaaacga cgcgtccacc accacatgca cgccctgact
     2221 gggcgcgacg atgggcgcgg cgggctggcc agcggccgcc gcatcctgct ggcgcagcgc
     2281 atcgacccac acgcccgtgg cgttaacgat acagccagcc tggatgcgga attcgcgccc
     2341 gctttcagcg tcgcgcacgc gcagacccgc agcgcggccg gcctcgtgca gcacctcgaa
     2401 ggcttcgcag taattgacca tcagcgcgcc gtgcgccgcc gccgtgcggg caatcgccag
     2461 cgccagccgg gcgtcgtcga actggccgtc ccaatacttc acaccgccct tcagcccgcg
     2521 cgactgggcg cccggcagca gctgcagcgc ctcgcgggcg ttcagccact cggtgcggcc
     2581 caggcccgcg ctgccggcga gcgcgtcgta catcttcagg ccgacgccgt aaaagggcgt
     2641 ctcccacagc ttgtacgagg gcatgacaaa cggcagcggc tgcgccaggt gcggcgcgct
     2701 gtgcaggatg tgggcgcgct cgtgcagcgc ctcgcgcacc agcgggatgt tgccctgcgc
     2761 cagataacgc acgccgccgt gcaccaattt tgtagcgcgc gaagaggtgc ccttggcaaa
     2821 gtcgtgcgcc tcgatcagca ccacgctgtg gccgcgctgc gcggcgtcca gcgccacgcc
     2881 caggcccgtg gcgccgccgc cgaccacggc gatgtcgtat ttgcgcggct cggccaggcg
     2941 ttccagcagc gcggcgcggt cggtgggcag ggggacgggg ctcaaggggc gcttccggtg
     3001 actatgtttt tgatagctgc ttgcgcttgt ccatcaagca ctgggtcttg ttttgaccat
     3061 ggatatacga cgcagcagca gaccacccat catgccagcg ggcggtggcg tcgcgcccat
     3121 ggccgcgcag caggcccgcg cgggaacgcc gcgctcgggc tctccccgag cgccagcgtt
     3181 gtccccttgg ggggaaggcg ccaaaggcga ctcagggggg atcatttttg gcccagccgc
     3241 gcgagcgcgc caccgcctcg cgccagcggg cgatgcgcgc cagccgctcg ggttcgggca
     3301 ggcgcggctc gaaacgccgg tcgacctgcc attgcgcggc gatctcgtca ccgccagccc
     3361 acacgccggt cgccaagccc gccagataag ccgcgcccag tgccgtcgtc tcggtcacct
     3421 gcgggcgcac cacgggcacg ccgagcaggt cggcttgcat ctgcatcagc aagtcgttgc
     3481 ggctggcgcc gccgtccacg cgcagctcgg tcagctggat gcccgaatcg ctgaccatgg
     3541 cgccaaacaa atcggccgat tgcagcgcaa tggcttccag cgcggcgcgg gcgatgtgcg
     3601 cgcgcgtggt gccgcgcgtc agccccagca gcgtgccacg cgcacggccg tcccagtcgg
     3661 gcgcgcccag gccggcgaac gctggaacaa gaaatacgtc gccagcgctg gccacgcttg
     3721 cggcaagcgc ttcgatttct gaagcgctct ggatcatctg caagccgtcg cgcagccact
     3781 gcacggtggc gccggccgtg aacacactgc cttccaggca atacgcggta cggggcgcgc
     3841 tggcctgctc gggcccttgc cagccgacgg tggtcaagag ctggttgcgg cttttcaccg
     3901 ccacgctgcc ggtgttcatc agcatgaagc agcccgtgcc gtaagtgttc ttggccatgc
     3961 cgggcgcaaa gcaggcctgg ccgaaggtgg cggcctgctg gtcgcccgcg acgccggcaa
     4021 tcgggatggg cgcatcgccc agcacgcccg gcgcggtgtc gcccagcaca ccgctggagg
     4081 gcacgatgcg cggcagcacg ctgcgcggga tgtggaacag gcgcagcagt tcgtcatccc
     4141 acgccagcgt gtggatgttc atcagcatgg tgcgcgcggc gttgctggcg tcggtggcgt
     4201 gcacgcggcc ttgcgtgagc tgccagatca gccagctgtc cacggtgccg aaggccagtt
     4261 cgcccgcctc ggcgcgcgca cgggcgccgg gcgtttgatc cagcagccat tccagcttgg
     4321 tggccgagaa gtacgcgtcc agcaccagcc cggtcttgcg ctggatcagc ggcgccttgc
     4381 cctggctgcg cagcacctcg cagcgcgcgg tggttcggcg gtcttgccag acgatggccg
     4441 gcgccaccgg ctcgcccgtg gcgcggtccc acagcaccgt ggtttcgcgc tggttggtga
     4501 tgctgattgc tatgattagg tgagctgtct gcgcttgtcc cgactgcgct ggcggcagtt
     4561 tttgcacaca ttcgcgcgct acggccagtt gcgtcgccca gatctcggcg gcgtcgtgct
     4621 cgacccagcc cggctgcgga aaatgctgcg caaactcgcg ctgcgccagc gccgccacgc
     4681 ggccctgggt gtcgaaggcg atggcgcggc tgctggtggt gccctggtcg agcgcgagga
     4741 tgtagcgcat ggtggcccca agcgtagccg atcgcgccgg cacgactcaa gcccgctgcc
     4801 actaaactgc gccccgatgc acccgcccgg ctcgcccccc accctgcccc gacgcacctt
     4861 gctgcgcggc gccgcagcgg ccgccgtcgt cggcgccgcc gccttcg
//
LOCUS       DAOMVO010000185         4907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4847_length_4907_cov_14.794518, whole genome shotgun sequence.
ACCESSION   DAOMVO010000185 DAOMVO010000000
VERSION     DAOMVO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4847_length_4907_cov_14.794518"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKV30_09305"
     CDS             <1..293
                     /locus_tag="PKV30_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL30444.1"
                     /translation="MRGLVQRLRHADRKIESLALMDVYGRVARSLLEFASETADGELL
                     IREKISRQDIAKMVGASREMVSRVMKDLEERGFIQTQDNGSIVVKERLNTLG"
     gene            387..2723
                     /locus_tag="PKV30_09310"
     CDS             387..2723
                     /locus_tag="PKV30_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRL30445.1"
                     /translation="MAYPLNTFNDAVPAPASRSGVGRFAQEVFLVLGLALIIFVVMAL
                     ISYSPQDAAWSTSGASGPPHNWAGKLGAWLADVAFVLLGFSAWWCVAVAISAWIGGVR
                     RWMRGDRPTVSAPRSLWMRLAGTRWAFLLGLLLVLAASTGLEWSRLYRWDDALPGSAG
                     GILGYLGGPIAVKWLGFTGSALLGIAVLVLGVALVFQFSWSHVAERIGGALMALVESR
                     RERREIAQDLALGKQAAREREEVVHHEREVIEEQHPTPVLIEPAVVQVAKSERVVKEK
                     QKPLFTELPDSRLPQVDLLDSAPGRQESVAPETLEMTSRLIEKKLKDFGVEVRVVAAM
                     PGPVITRYEIEPATGVKGSQVVNLAKDLARSLSLVSIRVIETIPGKNYMALELPNAKR
                     QSIRLSEILGSHVYNDARSLLTMGLGKDIVGNPVVADLAKMPHVLVAGTTGSGKSVGI
                     NAMILSLLYKAEPKDVRLLMIDPKMLEMSVYEGIPHLLAPVVTDMKQAANGLNWCVAE
                     MERRYKLMSKAGVRNLAGFNQKIDEAKARGEFIYNPFSLTPEDPEPLDRLPHIVVVID
                     ELADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRISFQ
                     VSSKIDSRTILDQMGAEALLGMGDMLYMPSGTGFPQRVHGAFVSDDEVHRVVEYLKSQ
                     GEPNYVDGVLEGGTVDGEEGGFGDAGEGGGEKDPMYDQAVEVVLKNRKASISLVQRHL
                     KIGYNRAARLVEDMEKAGLVSSMSSSGQRDILVPARAE"
     gene            2836..3513
                     /gene="lolA"
                     /locus_tag="PKV30_09315"
     CDS             2836..3513
                     /gene="lolA"
                     /locus_tag="PKV30_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HRL30446.1"
                     /translation="MRQLFQHHSSFARLSAAAAVLALSAPMAWAGGLDSLAQFVKGTR
                     SGKAAFTQVVTSPAKDGQAPRAKTQSGTFEFQRPGKFRFVYAKPFEQTIVADGKTLWL
                     HDVDLNQVTARQQEQVLGATPAAIVAAAPDLKALEKDFTLTEEPDSGGQQWVKAVPKS
                     RDGQLQSIRVGLKPAAGGAELGTLDILDSFGQRSVLTFSNFEVNAAMPASHFQFTPPK
                     GADVLRN"
     gene            3627..>4907
                     /locus_tag="PKV30_09320"
     CDS             3627..>4907
                     /locus_tag="PKV30_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRL30447.1"
                     /translation="MARPTAASQDSTSLHVPLAERLRPRSLGEVVGQQHLLGEGMPLR
                     IAFESGQPHSCILWGPPGTGKTTIARLMADAFDAQFIPISAVLGGVKDIREAVELAQK
                     ARDGLSPQATIVFVDEVHRFNKSQQDAFLPHVESGLFTFIGATTENPSFEVNSALLSR
                     AAVYVLQPLADDDLRQILARAQAGQAQPAIESEAAERLIAYADGDARRLLNTLETLFV
                     AARAEHIDTITDAWLLKVLGERLRRYDKGGEQFYDTISALHKSVRGSDPDAALYWFAR
                     MLDGGADPRYLARRMIRMASEDIGLADPRALRIALDAADVYERLGSPEGELALAECVV
                     YLAVAPKSNAVYKAFGAARAFVKQDTTRPVPLHLRNAPTRLMKQLDYGKGYRYAHDEE
                     GAFAAGERYLPDGMAAQQFYQPVPRGLEIKIGEKL"
BASE COUNT          866 a         1598 c         1632 g          811 t
ORIGIN      
        1 tgatgcgcgg cctggtgcag cgcctgcgcc acgccgaccg caagatcgaa tcgctcgcct
       61 tgatggacgt gtacggccgc gtggcgcgct cgctgctcga gttcgcgagc gaaaccgccg
      121 atggcgagct gctgatccgc gaaaaaatct cgcgccagga cattgccaag atggtcggcg
      181 cctcgcgcga gatggtcagc cgcgtgatga aagatctgga agagcgcggc tttatccaaa
      241 cgcaagacaa cggctccatc gtggtgaagg aacgcctgaa caccctgggt tgatccgcgc
      301 ggggccggtc catcgatcgt caagcacctg agtacgccct tcgcggcggc tgcgtgcggt
      361 aagcttctca agctgtagtt tcatccatgg cttaccccct caatacattc aacgacgcgg
      421 tgcccgcgcc cgcctcgcgc agtggcgtcg ggcgctttgc gcaagaggtt tttctggtgc
      481 tcgggctggc actcatcatc ttcgtggtga tggcgctgat cagctactcg ccgcaagatg
      541 ctgcctggtc gacatccggt gccagcgggc cgccgcacaa ctgggccggc aagctgggcg
      601 cctggctggc cgatgtggct tttgtgctgt tgggtttttc cgcctggtgg tgcgtggccg
      661 ttgcgatcag cgcgtggatc ggcggggtgc ggcgctggat gcgcggcgac cggccgaccg
      721 tcagcgcacc gcgctcactc tggatgcgtc tggcgggcac gcgctgggcc ttcttgctgg
      781 ggctgctgct ggtgctggcg gccagcacgg gactggaatg gtcgcgcctg taccgctggg
      841 acgacgcact gcccggttcg gccggcggca tcctgggcta tctgggcggt ccgatcgcgg
      901 tcaagtggct cggctttacc ggctcggcac tgctgggcat cgccgtgctg gtgctgggcg
      961 tggcattggt gttccagttt tcgtggagcc atgtggccga gcgcattggc ggcgcgctga
     1021 tggccttggt cgagtcgcgc cgcgagcgcc gcgaaatcgc tcaagacttg gcgctgggca
     1081 agcaggccgc gcgcgagcgc gaggaggtgg tgcaccacga gcgcgaggtg atcgaggagc
     1141 agcaccccac gccggtgctg atcgagcccg ccgtggtgca ggtggccaag agcgagcggg
     1201 tggtgaagga aaagcagaaa cccctgttca ccgaactgcc cgacagccgc ttgccgcagg
     1261 tcgacctgct cgacagcgcg cctggccgcc aggaaagcgt ggcgcccgaa acgctggaga
     1321 tgaccagccg gctgatcgag aaaaagctca aggatttcgg cgtcgaagtg cgcgtggtgg
     1381 ccgccatgcc tggcccggtc atcacgcgct acgagatcga gccggccacc ggcgtcaagg
     1441 gctcgcaggt ggtcaacctg gccaaggatc tggcgcgatc cttgagcctg gtgtcgattc
     1501 gcgtcatcga gaccattccc ggcaagaact acatggcgct ggaactgccc aacgccaagc
     1561 gccaatcgat ccgcctgtcc gaaattctgg gctcgcacgt ctacaacgac gcgcgctcgc
     1621 tgctcaccat gggtctcggc aaggacatcg tcggcaaccc ggtggtggcc gatctggcca
     1681 agatgccaca cgtgctggtg gcgggcacca cgggctcggg caaatcggtc ggcatcaacg
     1741 ccatgatcct cagcctgctc tacaaagccg agcccaagga cgtgcgcctc ttgatgatcg
     1801 accccaagat gctggagatg tcggtctacg aaggcattcc gcacttgctg gcaccggtgg
     1861 tcaccgacat gaagcaggcc gccaatggcc tgaactggtg cgtggccgag atggagcggc
     1921 gctacaagtt gatgagcaag gcgggcgtgc gcaacctggc cggcttcaac cagaagatcg
     1981 acgaggccaa ggcgcgcggc gagttcatct acaacccctt cagcctgacg cccgaagacc
     2041 ccgagccgct cgatcgcctg ccgcacatcg tggtggtgat cgacgagctg gccgacctga
     2101 tgatggtggt cggcaagaag atcgaagagc tgatcgcccg cctggcgcaa aaggcacgcg
     2161 cggccggcat tcacctgatt ctggccacgc agcggcccag cgtggatgtg atcacgggcc
     2221 tgatcaaggc caatattccg acccgtattt cgttccaggt ttccagcaag atcgacagcc
     2281 gcaccatcct cgaccagatg ggcgccgagg cgctgctggg catgggcgac atgctgtaca
     2341 tgcccagcgg caccggcttt ccgcagcgcg tgcacggcgc gtttgtcagc gatgacgagg
     2401 tgcaccgcgt ggtcgaatac ctcaagagcc agggcgaacc caactacgtc gatggcgtgc
     2461 tcgaaggcgg caccgtcgat ggcgaggagg gcggctttgg cgacgcgggc gagggcggcg
     2521 gcgagaaaga cccgatgtac gaccaggccg ttgaagtggt gctgaagaac cgcaaggcca
     2581 gcatctcgct ggtgcagcgc cacctgaaga tcggctacaa ccgcgccgcg cggctggtcg
     2641 aggacatgga gaaggcgggc ctggtcagct ctatgagctc cagcggccag cgcgacattc
     2701 tggtgccggc gcgcgccgag tagcgcccat cagcgcgccc gccaacagcc accacggttg
     2761 taggccggtg cctcaacttt gcgattcttg acctggcggt gccgaacctg gcgccagaat
     2821 gccgctctta tgcacatgcg ccaacttttt caacaccatt cctccttcgc gcgcctgtcc
     2881 gctgcggcgg ccgtgctggc cttgagtgcg cccatggcct gggccggtgg cctcgacagc
     2941 ctcgcccagt tcgtcaaggg cacgcgctcg ggcaaggccg cgttcaccca agtcgtgacg
     3001 tcgccagcca aggacggtca ggcgccgcgc gccaagacgc aaagcggcac cttcgagttc
     3061 cagcgccccg gcaaattccg ctttgtctac gccaagccgt tcgagcagac catcgtcgcc
     3121 gacggcaaga cgctgtggct gcacgacgtc gatctgaatc aggtcacggc gcgccagcaa
     3181 gagcaggtgc tgggcgccac gcccgccgcc atcgtggccg cggcgcccga cctgaaggcg
     3241 ctggagaagg atttcaccct gaccgaagag ccggacagcg gcggccagca atgggtcaag
     3301 gcggtgccga aatcgcgcga cggccagttg caaagcattc gcgtcggcct caagcccgcc
     3361 gccggcggtg cggaactggg cacgctggac attctggaca gctttggcca gcgctcggtg
     3421 ctgacgttca gcaatttcga ggtcaacgcc gccatgccgg ccagccactt ccagttcacg
     3481 cctcccaagg gggcggatgt gctgcgcaat tgatttttga atcaaacagg cctctggcgc
     3541 ttgatgcaca agcgcgagca gctatcaaat atgtagtttc aaagtgggtg gatggcacgt
     3601 gggacgcgcg tttctagaat gtgcccatgg cccgccccac cgccgcttcg caagactcca
     3661 cttcgctgca cgtgcccctg gctgagcgcc tgcgcccgcg cagcctgggc gaagtggtcg
     3721 ggcagcagca tttgttgggc gagggcatgc cgctgcgcat cgcttttgaa tccggccagc
     3781 cgcacagctg catcctgtgg ggaccgccgg gcaccggcaa gaccaccatc gcgcggttga
     3841 tggccgatgc gttcgacgcg cagttcatcc ccatcagcgc ggtgctgggc ggcgtcaagg
     3901 acatccgcga agcggtggaa ctggcgcaga aggcgcgcga tggcctgagc ccgcaggcca
     3961 ccatcgtctt cgtcgacgag gtgcaccgct tcaacaagag ccagcaggat gccttcttgc
     4021 cgcacgttga aagcggcttg ttcaccttca tcggcgccac caccgagaac ccgtcgttcg
     4081 aggtcaactc ggcgctgctg tcacgcgccg ccgtctacgt gctgcagccg ttggcagacg
     4141 acgatttaag gcaaattctg gcccgggcgc aagcggggca agcgcaacca gctatcgaaa
     4201 gcgaagcggc cgaacgcttg atcgcctacg ccgacggcga cgcgcgccgc ctgctgaaca
     4261 cgctggagac actgttcgtc gccgcccgcg ccgagcacat cgacaccatc accgacgcct
     4321 ggctgctcaa ggtgctgggc gagcgcctgc gccgctacga caagggcggc gagcagttct
     4381 acgacaccat cagcgcgctg cacaagtcgg tgcgcggctc ggatcccgat gcggcgctgt
     4441 actggttcgc ccgcatgctc gacggcggcg ccgacccgcg ctacctggcg cgccgcatga
     4501 tccgcatggc cagcgaagac atcggcctgg ccgacccgcg tgcgctgcgc atcgcactcg
     4561 acgcggccga cgtctacgag cgcctgggct cgcccgaggg cgaactggcg ctggccgaat
     4621 gcgtggtcta cctggccgtg gcgcccaaga gcaacgccgt ctacaaggcc tttggcgccg
     4681 cgcgcgcctt cgtcaagcaa gacaccacgc gccccgtgcc gctgcacctg cgcaacgcgc
     4741 cgacccggct gatgaagcaa ctcgactacg gtaagggcta ccgctacgcg catgatgaag
     4801 aaggtgcgtt tgccgccggc gagcgttatc tgccggacgg catggcggcg cagcagttct
     4861 atcaacccgt gccgcgcggg ctggagatca agatcggtga gaagctc
//
LOCUS       DAOMVO010000186         4864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4907_length_4864_cov_14.337492, whole genome shotgun sequence.
ACCESSION   DAOMVO010000186 DAOMVO010000000
VERSION     DAOMVO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4907_length_4864_cov_14.337492"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2333
                     /gene="xdhB"
                     /locus_tag="PKV30_09325"
     CDS             <1..2333
                     /gene="xdhB"
                     /locus_tag="PKV30_09325"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRL30448.1"
                     /translation="PGVRALITAADIPGDPVLATFVHDEPVFAKDVVQHAGQVVGLIV
                     ADTVQAARHAARQVQLDIAPLPPILTIEQAMAAQSFVLPTVTVTRGDPSAALGTSPHR
                     LQGQFAVGGQEHFYLEGQIALAQPREQDQWHITSSTQHPGEVQHWVAHALGIASHRVT
                     VECRRMGGGFGGKETQAGHIAVWAALAALKTKAPVKLRLDRDDDFLITGKRHPFAYDY
                     DVGFDDAGRITGLSLDMMANCGFSADLSGPVCDRAVFHADNAYFLSDVIIRSHRCKTN
                     VQSHTAFRGFGGPQGVIAIETILGDIARALGKDALDVRIANLYDGRTPSPAGGGQGWG
                     QPAPTSNATLLTPALSPEGRGSHTAAPDLLPLPPAGEGRGEGAASPKRDTTPYQMRVD
                     DNILPNLLPKLELLAQYRRRQAAVSAWNSKNRHLKRGIAITPVKFGISFTATLFNQAG
                     ALVHVYTDGSVQVNHGGTEMGQGLHTKVAQVVADELGVPLPQVLVTGSATDKVPNASA
                     TAASSGTDLNGKAAQAAARTVRDNLAAFVCGLDGCGAGAVEFRGGQVITPKTTRAFAD
                     VVKEAYANRIQLWSDGFYRTPKIHYDKTTLTGRPFFYFAYGAACSEVAIDTLTGEYRV
                     LGVDILHDVGRSINPAIDIGQIEGGFIQGMGWLTTEELVWDDKGQLTTKAPSTYKIPT
                     GADLPPHFNVHLWPEPNDEDTIFRSKAVGEPPFMLAISVWEAMRNAVAAAREGATADA
                     RAPVRMDAPATPERVLRALGTVPLSDAGPVVVPVPT"
     gene            2416..3072
                     /locus_tag="PKV30_09330"
     CDS             2416..3072
                     /locus_tag="PKV30_09330"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRL30449.1"
                     /translation="MEWWAIAAVAAALGGVGWWWWSRRPPADGARKDRYAAEAVITPA
                     QAKLYRHLQEAFPDQVVLFAQPLSQLVSVRHAGDRQRALQRLRDQVVDFVVCAPDGKP
                     SFAFQVDAYRSGDEDQARRDAALKHRVLATAGVRMLRLKKSVRHLPSPQELRQRLEAT
                     SLANAPEADLKEAVPPSWRAEQVPVLTDRIRSRHGDHAEATDSMSLTDLMGLPPASAR
                     "
     gene            complement(3120..3196)
                     /locus_tag="PKV30_09335"
     tRNA            complement(3120..3196)
                     /locus_tag="PKV30_09335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3160..3162),aa:Met,seq:cat)
     gene            complement(3247..4026)
                     /locus_tag="PKV30_09340"
     CDS             complement(3247..4026)
                     /locus_tag="PKV30_09340"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRL30450.1"
                     /translation="MAYEFITTRTEGEKVGIITLNRPKQLNALSPALMVELGQALKGF
                     DADDAIGCIVITGNEKAFAAGADIGAMATYTFADVYRDDYITRDWETIRSIRKPVIAA
                     VSGFALGGGCELAMMCDFIIAADNARFGQPEIKLGIIPGAGGTQRLPRAVGKSKAMDL
                     ALTGRMMDAAEAERAGLVSRVVPLDKLMDETLAAALMISGFGRMAVMAAKECVNRAYE
                     GTLADGVMFERRMFHALFATADQKEGMDAFVNKRAAVFKNQ"
     gene            4149..>4864
                     /locus_tag="PKV30_09345"
     CDS             4149..>4864
                     /locus_tag="PKV30_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRL30451.1"
                     /translation="MTTAAPSTTAEPTVLFAHEGPIAILTLNRPASLNSFTRQMHREL
                     WAALDKVEADRSIRALVLTGAGRAFCAGADLSEFDFRAGDDIMFRADPGPVIDQAFNP
                     TTRRLMAVRVPTVCAVNGVAAGAGASVAMACDIAIAAPGASFSQAFSKIGLVPDSGGT
                     WLLPQRVGLARAMALCMTGDKLPAADAKAMGMIWDVADDAVAAAKAMAEKLAAMPTKA
                     LVATRALLRGAHTHSLDEQL"
BASE COUNT          828 a         1658 c         1601 g          777 t
ORIGIN      
        1 tgcctggggt gcgcgctctc attaccgcag cagacatacc gggcgacccg gtgctggcca
       61 cattcgtgca cgacgagccc gttttcgcca aagacgtcgt gcaacacgcc ggccaggtgg
      121 ttggcctgat cgttgccgac accgtgcagg cggcgcgcca cgccgcgcgc caggtgcagc
      181 tcgacatcgc gccgctgccg ccgatcctga cgattgagca agccatggcc gcccagagct
      241 tcgtgctgcc cacggtgacc gtcacgcgcg gcgatccatc agccgcgctc ggcacatcgc
      301 cgcaccgcct gcagggccag ttcgccgtcg gcgggcaaga gcacttttat ctggaaggcc
      361 agatcgccct ggcccagccg cgcgagcaag accagtggca tatcaccagc agcacgcagc
      421 acccgggcga ggtgcagcac tgggtcgcgc atgcgctggg cattgccagc caccgcgtca
      481 ccgtcgaatg ccggcgcatg ggcggtggct ttggcggcaa ggagacgcag gccggccaca
      541 tcgccgtgtg ggcggctctg gccgctctca aaacaaaagc gccggtgaag ctgcggctgg
      601 accgcgacga cgatttcctc atcaccggca agcggcaccc gtttgcgtac gactacgacg
      661 tcggctttga cgacgcgggc cgcatcaccg gcttgtcact cgacatgatg gccaactgcg
      721 gcttctcggc cgatttgagc ggcccggtgt gcgaccgcgc cgtcttccac gccgacaacg
      781 cttatttttt gagcgacgtg atcatccgca gccaccgctg caagacgaat gtgcaaagcc
      841 acacggcgtt tcgcggcttc ggcggtccgc agggcgtgat cgccatcgag acgattctgg
      901 gcgacatcgc gcgagcattg ggcaaggatg cgctcgatgt gcgcatcgcc aacctgtacg
      961 acggacgcac tccctccccc gctgggggag ggcaggggtg ggggcagccc gcgcccacct
     1021 cgaacgccac gctcctcacc ccagccctct ccccagaggg gcgagggagc cataccgccg
     1081 cgcctgattt gctccctctc ccgcctgcgg gagagggcag gggtgagggt gcagcttcgc
     1141 ccaagcgcga caccaccccc taccagatgc gggtcgacga caacatcctg cccaatttgc
     1201 tcccaaaatt ggagctcctg gcgcagtatc gacggcgaca agcggctgtt tcggcttgga
     1261 actcgaaaaa ccggcacctc aagcgcggca tcgccatcac gcccgtcaag ttcggcatca
     1321 gcttcaccgc cacgctgttc aaccaggccg gcgcgctggt gcacgtctac accgacggca
     1381 gcgtgcaggt gaaccacggc ggcaccgaaa tgggccaggg cctgcacacc aaggtggcgc
     1441 aggtggtggc cgacgaattg ggcgtgccgc tgccgcaggt gctggtcacg ggcagcgcca
     1501 ccgacaaggt ccccaacgcc agcgccaccg ccgcatcgag cggcaccgat ttgaacggca
     1561 aggccgcgca ggcggcagcg cgcaccgtgc gcgacaacct ggcggcgttc gtctgcgggc
     1621 tggacggctg cggcgccggc gcggtcgaat tccggggtgg ccaggtcatc acgcccaaga
     1681 ccacgcgcgc ctttgccgac gtggtgaaag aggcctatgc caaccgcatc cagctgtgga
     1741 gcgacggctt ctatcgcacg cccaagatcc actacgacaa gaccacgctc accggccggc
     1801 cgttcttcta cttcgcctac ggcgccgcct gcagcgaggt cgccatcgac acgctgacgg
     1861 gcgaataccg cgtgctgggc gtggacatcc tgcacgacgt tggccgcagc atcaacccgg
     1921 ccatcgacat cggccagatc gaaggcggtt tcatccaggg catgggctgg ctgacgacgg
     1981 aagaactggt gtgggacgac aagggccagc tcaccaccaa ggcgcccagc acctacaaga
     2041 tccccaccgg cgccgacctg ccgccgcatt tcaacgtgca cctgtggccc gagccgaacg
     2101 acgaagacac catcttccgc agcaaggccg tgggcgagcc gcctttcatg ctggcgatca
     2161 gcgtgtggga ggcaatgcgc aatgccgtgg ctgccgcccg cgaaggcgca accgcagacg
     2221 cgcgcgcgcc ggtgcgcatg gacgcgccgg ccacgccgga gcgggtgctg cgcgcgctgg
     2281 gcaccgtccc gctgagcgac gcaggcccgg tcgtggtgcc agtgccgacg tgagttttta
     2341 ctcactttgt gctagaacgc gcgttttgat cgggcgacgc gcacgctgcc ggaacagcgc
     2401 aaggagcaag acacgatgga atggtgggca attgcagccg tggcagcggc actcggcggc
     2461 gtgggctggt ggtggtggtc gcgccgcccg ccggccgatg gcgcgcgcaa ggatcgctac
     2521 gccgccgaag cggtgatcac gccggcgcag gccaagctgt accggcacct gcaagaggct
     2581 tttcccgacc aggtggttct gttcgcgcaa ccgttgagcc agctggtgtc ggtcaggcat
     2641 gccggcgacc gccagcgtgc gctgcagcgc ttgcgcgatc aggtggttga tttcgtcgtc
     2701 tgcgcgccag acggcaagcc cagcttcgcc ttccaggtcg acgcttaccg aagcggcgat
     2761 gaagaccagg cgcggcgtga cgccgccctc aagcaccgcg tgctggcgac cgctggcgtg
     2821 cgcatgttgc ggctgaagaa atcggtccgc cacctgccgt cgccgcagga attgcggcag
     2881 cggctcgaag ccacgtcgct ggccaatgcg cccgaggccg acctgaaaga ggcggtgccc
     2941 ccaagctggc gcgccgaaca ggtgccggtg ctgaccgacc gcatccgctc gcgccatggc
     3001 gatcatgccg aagccaccga cagcatgagc ctgaccgacc tgatgggctt gccgcccgcg
     3061 tcggcgcgct gaaagaaaaa ggccctgcga acatcgagtt cgcagggccc tcttggattt
     3121 ggtggtggta ggtggaatcg aaccaccgac cttggggtta tgaatcccac gctctaaccg
     3181 actgagctat accaccggca accgcgcatt atatatgtct gtcggtcatg ccttgctgcg
     3241 gcgccgtcac tgattcttga acaccgccgc gcgcttgttg acgaaggcgt ccatgccttc
     3301 cttctggtcg gcggtggcaa acaacgcgtg gaacatgcgg cgctcgaaca tcacgccgtc
     3361 agccagggtg ccttcatacg cgcggttgac gcattccttg gccgccatca ccgccatgcg
     3421 gccaaagccg ctgatcatca gcgcggcggc cagggtttcg tccatcagct tgtccagcgg
     3481 caccactcgg ctgaccagac cggcgcgctc ggcctcggcg gcgtccatca tgcggccggt
     3541 gagcgccagg tccatcgcct tcgatttacc caccgcgcgc ggcaggcgct gcgtgccgcc
     3601 ggcgccgggg attatgccga gcttgatttc gggctggcca aacctggcgt tgtccgcggc
     3661 aatgatgaag tcgcacatca tcgccagctc gcagccgccc cccagcgcaa acccgctgac
     3721 ggcggcaatc accggcttgc ggatgctgcg gatggtttcc cagtcgcgcg tgatgtagtc
     3781 gtcgcggtag acgtcggcaa aggtgtaggt ggccatggcg ccaatgtcgg cgccggcggc
     3841 aaaggccttc tcgttgccgg tgatgacgat gcagccgatg gcgtcgtcgg cgtcaaagcc
     3901 tttcagcgcc tggcccagtt ccaccatcag cgccgggctg agggcgttga gctgcttggg
     3961 ccggttcagc gtgatgatgc cgaccttctc gccctcggtg cgggtggtga tgaattcgta
     4021 ggccatgggg ttctccttgt gtgatgtggg tgcgttcgct ggcactataa ccagcgatga
     4081 cagccgcctg aagttaaccg accgctgagt cggttaatgg taaattgatg cacgaggaga
     4141 caagccacat gaccactgca gcgcccagca ccacggctga gcccaccgtt cttttcgcgc
     4201 acgaaggccc catcgccatc ctgacgctga accgccccgc ctcgctcaac agcttcacgc
     4261 gccagatgca ccgcgagctg tgggctgcgc tcgacaaggt cgaggccgac aggtcgatcc
     4321 gcgcgttggt gctgaccggt gccggccgcg cgttttgcgc cggcgccgat ctgtcggagt
     4381 tcgactttcg cgcgggcgac gacatcatgt tccgcgccga tcccggcccg gtgatcgacc
     4441 aggctttcaa ccccacgacg cggcgcctga tggcagtgcg cgtgcccacc gtgtgcgcgg
     4501 tcaacggcgt cgcggcgggc gcaggcgcat cggtggccat ggcgtgtgac atcgccattg
     4561 ccgcgccggg cgccagcttc agccaggcct tcagcaagat cgggctggtg ccggattcgg
     4621 gcggcacctg gctgctgccg caacgcgtgg gcctggcgcg cgccatggcg ctgtgcatga
     4681 cgggcgacaa actgcccgct gccgacgcca aggccatggg catgatctgg gatgtggccg
     4741 acgatgccgt ggccgccgcc aaggccatgg ctgaaaagct ggctgccatg cctaccaagg
     4801 cgctggtcgc cacgcgcgcg ctgctgcgcg gcgcgcacac gcacagcctg gacgagcaac
     4861 tcga
//
LOCUS       DAOMVO010000187         4861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4914_length_4861_cov_16.976488, whole genome shotgun sequence.
ACCESSION   DAOMVO010000187 DAOMVO010000000
VERSION     DAOMVO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4914_length_4861_cov_16.976488"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKV30_09350"
     CDS             <1..476
                     /locus_tag="PKV30_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRL30452.1"
                     /translation="AARLLGRPYSISGHVVHGRKLGRALAETAPGKADGFRTLNLRFS
                     RRAHAWKPAASGIFVVQVHGLTDQPLAGVANLGVRPSLDPNDVNGGRVLLETHVLDWP
                     AHLGQEGAYGKIIRVDLLHKLHDELRYHSLAALTEGIARDCADARAWLAADGGRI"
     gene            604..3438
                     /gene="ileS"
                     /locus_tag="PKV30_09355"
     CDS             604..3438
                     /gene="ileS"
                     /locus_tag="PKV30_09355"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRL30453.1"
                     /translation="MSTENKTDYRKTLNLPDTPFPMRGDLPKREPGWIKEWDDMGLYK
                     RLRLARAGREKFILHDGPPYANGQLHAGHAVNKILKDMIVKTRQLKGLDAHYVPGWDC
                     HGLPIENQIEKLYGRNLSRDEMQAKGRAYATEQIALQMQDFKRLGVLGEWDNPYKTMN
                     KPNEAGELRLFKRLMERGFVYRGLKPVYWCFDCGSALAEAEIEYQDKKSKTVDVAFEA
                     AEPDKLAAAFGLPKLDKPAFIVIWTTTAWTIPANQALNVGPHIEHALVDTPRGLLVLA
                     KARVEDALKRYGIEGTIIATAMGDKLGGLNFKHPLYDVDKGYQRLSPVYLADYATDTD
                     GTGIVHSAPAYGIEDFNSCVSHGLAHADILNPVQGGGSYAGDFPLFGGLDIWKAVPVI
                     IKTLDDAGRLLATSTITHSYPHCWRHKTPVIYRAASQWFVRMDEGEGVFTKDKAPETL
                     RTTALRAIEDTAFYPPSGKARLRDMIAGRPDWVISRQRAWGVPIAFFLHKETDELHPR
                     TMEILDQAADIIDQGGIEAWSRVTPEEILGDEDARHYRKFNDILEVWFDSGSTFDHVL
                     CGTHPNEHHTSGPEADMYLEGHDQHRGWFHSSLLLASAIRGRAPYRSLLTHGFTVDAQ
                     GRKMSKSLGNGIDLQEANKKWGAEILRLWVAASDYSGDITGDDKIMARVIDAYRRIRN
                     TLRFLLANTSDFDPVKDAVAEGDLLEIDRWLLSRAAEFQADVLAHYEAYEFHPVVAKL
                     QLFCSEDLGGFYLDVLKDRLYTTAPHSLARRSAQTALWRLTQAMLRWMAPFLSFTAEE
                     AWKLVGQGDSIFLAEFSEFAAPDAALLAKWARIREIREAVNKDIEVLREKGEVGSSLQ
                     ANVVLTAPSADHALLATLGDDLRFVFITSAIELEAGNDLSVSASASKDVKCERCWHYV
                     ADVGADAEHPTLCGRCISNLYGAGETRKVA"
     gene            3440..3955
                     /gene="lspA"
                     /locus_tag="PKV30_09360"
     CDS             3440..3955
                     /gene="lspA"
                     /locus_tag="PKV30_09360"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRL30454.1"
                     /translation="MARGKSAGASLALWLALAALVVVLDQFTKQLILGNYQLGDSTYV
                     TSFFNIVRAHNTGAAFSFLAGAGGWQRWFFVALAVGASLFIVWLLRSHPGQRLFAFSL
                     SMILGGAVGNVIDRLMHGYVVDFLDFHWSFLSPMFYRGHFPAFNVADAAITAGAIGLI
                     LDELLRVRRGR"
     gene            4020..4580
                     /locus_tag="PKV30_09365"
     CDS             4020..4580
                     /locus_tag="PKV30_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HRL30455.1"
                     /translation="MPTFAEQNLAAALGRNWWLLLVRGLVAIVFALLTWAQPGVSLAA
                     LVLVFGIYVLADGLLGVWSAIAKRRDNRHWWLLLLWGLVGIVVGVMTFIMPGITGLVL
                     LMYIAAWAVITGVLQIVAAIRLRKEIKGEWLMILSGLVSVAFGVLLFLQPGAGALAVA
                     WIIAAYAFIFGVLMVLLAFKVRKLGR"
     gene            complement(4660..>4861)
                     /locus_tag="PKV30_09370"
     CDS             complement(4660..>4861)
                     /locus_tag="PKV30_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30456.1"
                     /translation="YESSNELAVRMNDDTVSRLQGWFDEELRRSQHVFPFHSMPELVR
                     FDFGAWDVIERDYKGSVYLRLR"
BASE COUNT          848 a         1610 c         1570 g          833 t
ORIGIN      
        1 acgcggcacg gctgctgggc cggccgtatt cgatcagcgg ccacgtggtg cacggccgca
       61 agctgggccg cgcgctggct gaaacggcgc cgggcaaggc ggacggcttt cgcaccctca
      121 acctgcgctt ttcacgccgc gcccatgcgt ggaagcccgc cgccagcggc atcttcgtgg
      181 tgcaggtgca cggcttgacc gaccagccgc tggccggcgt ggccaacctg ggtgtgcggc
      241 cctcgctcga tccgaacgac gtgaacggcg gccgcgtgct cctggagacg catgtgctcg
      301 actggccagc gcacttaggg caagaggggg cctacggtaa aatcatccgg gtggacctgc
      361 tgcacaaact gcacgacgaa ttgcgctatc acagcctggc ggcgctgacc gaaggcatcg
      421 cgcgcgactg cgccgacgcg cgcgcctggc tggccgcgga cggcggccga atttgacggg
      481 gcggcggcac gccccagcgc ctgtccgcac ccagcgcccc actccttatt tcatagcttc
      541 tcgcgcttga tctgcttgcg ccagcagccg atttgattca aattccctcc gagccttggc
      601 gccatgagca cagagaacaa gaccgactac cgcaagaccc tgaacctgcc cgacacgccc
      661 ttccccatgc gcggcgacct gcccaagcgc gagccgggct ggatcaagga gtgggacgac
      721 atgggcctgt acaagcgcct gcgcttagcg cgcgccggac gcgaaaaatt catcctgcac
      781 gacggcccac cgtatgccaa tggccagctg cacgccggcc acgcggtcaa caagatcctg
      841 aaggacatga tcgtcaagac ccggcagttg aagggcctgg acgcgcacta cgtgccgggc
      901 tgggactgcc acggcctgcc gatcgagaac cagatcgaga agctctacgg ccgcaacttg
      961 agccgcgacg agatgcaggc caagggccgc gcctacgcca ccgagcagat cgccctgcag
     1021 atgcaggact tcaagcgcct gggcgtgctg ggcgaatggg acaaccccta caagaccatg
     1081 aacaagccga acgaggcggg cgagttgcgc ctgttcaagc ggctgatgga gcggggtttt
     1141 gtctatcggg gcctgaagcc ggtgtactgg tgcttcgact gcggatcggc gctggccgag
     1201 gccgagatcg agtaccagga caagaagagc aaaacggtcg atgtggcgtt tgaagccgcc
     1261 gagccggaca aactggccgc cgcgttcggt ctgccgaagc tcgacaagcc cgccttcatc
     1321 gtcatctgga ccaccaccgc gtggaccatc cccgccaacc aggcgctcaa cgtcggcccg
     1381 cacatcgagc acgcactggt cgatacgccc cgtggcctgc tggtgctggc caaggcgcgg
     1441 gtggaagacg cgctcaagcg ctatggcatc gaaggcacca tcatcgccac ggccatgggc
     1501 gacaagctgg gtggcctcaa cttcaagcac ccgctgtacg acgtggacaa aggctaccag
     1561 cgcctgtcgc cggtctacct tgccgactac gccaccgaca ccgacggcac gggtatcgtg
     1621 cactcggcgc ccgcctacgg tattgaagac ttcaactcct gcgtgtcgca cggcctggcg
     1681 cacgccgaca tcctgaaccc ggtgcaaggc ggcggcagct acgcgggcga ctttccgctg
     1741 tttggcgggc tggacatctg gaaggccgtg cccgtcatca tcaagacgct ggatgacgcc
     1801 ggccgcttgc tcgccaccag caccatcacg cacagctacc cgcactgctg gcgccacaag
     1861 acgccggtga tctaccgcgc cgcctcgcaa tggttcgtgc gcatggacga aggcgaaggc
     1921 gtgttcacca aggacaaggc gcccgaaacg ctgcgcacca ccgcgctgcg cgccatcgaa
     1981 gatacggcct tctacccgcc cagcggcaag gcgcgcctgc gcgacatgat cgcgggccgg
     2041 cccgactggg tcatcagccg ccagcgcgcc tggggcgtgc ccatcgcctt cttcctgcac
     2101 aaggaaacgg acgagctgca cccgcgcacc atggaaatcc tcgaccaggc ggccgacatc
     2161 atcgaccagg gcggcatcga ggcctggagc cgcgtgacgc ccgaggaaat cctgggcgac
     2221 gaagacgcgc gccactaccg caagttcaac gacatcctgg aagtgtggtt cgactccggc
     2281 tccaccttcg atcacgtgct gtgcggcacg cacccgaatg agcaccacac gagcgggccg
     2341 gaagcggaca tgtatctgga aggccacgac cagcaccgcg gctggttcca ctcgtcgctt
     2401 ttgctggcca gcgccatccg tggccgcgcg ccgtatcgca gcctgctcac gcacggcttc
     2461 acggtggatg cgcagggccg caagatgagc aagtcgctcg gcaacggcat cgacctgcag
     2521 gaagccaaca agaagtgggg cgcggagatt ttgcgcctgt gggtggccgc cagcgactat
     2581 tcgggcgaca tcacgggcga cgacaagatc atggcgcgcg tcatcgacgc ttaccgccgc
     2641 atccgcaaca cgctgcgctt tctgctggcc aacaccagcg atttcgaccc cgtgaaagac
     2701 gccgtggccg agggcgatct gctggaaatc gaccgctggc tgctgtcgcg cgccgccgag
     2761 tttcaggccg atgtgctggc gcactacgag gcgtacgaat tccaccccgt ggtcgccaag
     2821 ctgcagctgt tctgctcgga ggacctgggt ggcttctacc tcgacgtgct gaaggaccgg
     2881 ctctacacca ccgcgcccca ttcgctcgcg cgccgcagtg cgcagaccgc gctgtggcgg
     2941 ctcacgcagg ccatgctgcg ctggatggcg cctttcttga gcttcaccgc tgaggaagcc
     3001 tggaagctgg tcgggcaagg cgactcgatt tttctggcgg agttttccga gtttgccgcg
     3061 cccgacgccg cgctgctcgc caagtgggcg cgcatccgcg agatccgcga agccgtcaac
     3121 aaggacatcg aggtgctgcg cgagaagggc gaagtcggct catcgctgca agccaacgtg
     3181 gtgctgacgg cgccgtcggc cgaccacgcg ctgctggcga ctttgggcga cgatctgaga
     3241 ttcgttttca tcacgtctgc tattgaattg gaagctggta acgatttatc cgtcagcgcc
     3301 agcgcctcga aagacgtgaa atgcgagcgc tgctggcact acgtggccga tgtgggcgcc
     3361 gatgccgaac acccgacgct ctgcggccgc tgcatcagca acctgtacgg cgcgggcgaa
     3421 acccgcaagg tcgcctgaag tggcgcgcgg caaatccgcg ggcgcgagcc tcgccctgtg
     3481 gctggcgctg gccgcgctgg tggtggtgct cgatcagttc accaagcaac tgatcctggg
     3541 caattaccag ctcggggaca gcacgtatgt gacgagcttc ttcaacatcg tgcgcgccca
     3601 caacaccggc gccgcgttct cgttcctggc cggcgccggc ggctggcagc gctggttctt
     3661 tgtcgcgctg gcggtgggtg cctcgctgtt catcgtctgg ctgctgcgct cgcacccggg
     3721 gcagcggctg ttcgccttct cactcagcat gatcctgggc ggcgccgtcg gcaacgtcat
     3781 cgaccggctg atgcacggct acgtggtgga ttttctagac ttccactggt cgttcttgag
     3841 cccgatgttc tatcgcgggc atttcccggc gttcaacgtg gcggatgcgg ccatcaccgc
     3901 cggcgccatc gggctcatcc tggacgagtt gttgcgcgtg cggcgcgggc gttgattgct
     3961 gggtgcggtc gcgggtacac tggccgctcc ttgttcttca gcaccgaaag gagcagccca
     4021 tgccgacgtt tgccgaacaa aatcttgccg ccgcgctggg ccgcaactgg tggctgctgc
     4081 tggtgcgcgg gctggtggcc atcgtgtttg ccctgctcac ctgggcgcaa cccggcgtgt
     4141 cgctggccgc gctggtgctg gtgttcggca tctacgtgct ggccgatggc ctgctgggcg
     4201 tgtggagcgc catcgccaaa cggcgcgaca accgccactg gtggctgctg ctgctgtggg
     4261 gcctggtcgg catcgtggtc ggcgtgatga ccttcatcat gcccggcatc accgggctgg
     4321 tgctgctgat gtacatcgcc gcctgggcgg tgatcacggg tgtgctgcag atcgtggccg
     4381 ccatccgcct gcgcaaggag atcaagggcg aatggctgat gattctgagc gggctggtct
     4441 cggtcgcctt cggtgtgctg ctgttcctgc aacccggcgc cggagcgcta gcggtggcct
     4501 ggatcatcgc ggcctacgcc ttcatcttcg gcgtgctgat ggtgctgctg gccttcaaag
     4561 tgcgcaagct ggggcgttga atcggcgacg caagttgcgc tgtacctttg caccacggcg
     4621 cagcaccacc gtcaaggcag cgcaggctat cccaggccgc taacgcaatc gaagatagac
     4681 gctgcccttg tagtcccgct caatcacgtc ccacgctcca aagtcgaaac gaacaagctc
     4741 cggcatcgaa tgaaagggaa agacgtgctg cgagcggcgc aactcttcgt cgaaccaccc
     4801 ctgcaagcga ctgactgtgt cgtcgttcat gcgaacggcc aattcgttgc ttgattcgta
     4861 g
//
LOCUS       DAOMVO010000188         4853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4926_length_4853_cov_15.060650, whole genome shotgun sequence.
ACCESSION   DAOMVO010000188 DAOMVO010000000
VERSION     DAOMVO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_4853_cov_15.060650"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="crcB"
                     /locus_tag="PKV30_09375"
     CDS             complement(<1..339)
                     /gene="crcB"
                     /locus_tag="PKV30_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRL30457.1"
                     /translation="MHAVFAICLGACVGALARWRLGLWLNHPGAFMPWGTLAANLIGG
                     YLVGLILAWAHHADLAPFWRLILVTGFLGALTTFSSFSAEVVEMALADRWGAALSTAA
                     LHLLGSLALTW"
     gene            complement(360..1370)
                     /locus_tag="PKV30_09380"
     CDS             complement(360..1370)
                     /locus_tag="PKV30_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HRL30458.1"
                     /translation="MFKNLMLYRLSPEWPTSAAQWEEALASEPFVECSATQQKSTGWV
                     PPRGQEHGALVETVDGQWIARFAIETKAVPADAVRARTQKVVEEIEKTTGRKPGKKEL
                     RDLKDDALIALLPQAFPRRSQVTVWIEPTQRWLVMDVGAQGKADEVITSLVRVAGQGF
                     GVGLLQTQQSPQGAMAAWLAAESAEDIPDAFNVERECELKGSGDEPAVVKFTRHDLAT
                     DEVRQHIAEGKLPTKLALGWQGRVGFMLTQALQLKKIAFQEGVFEEGAASKDDDRFDA
                     DVALSTGELSGLIADLIDALGGEADATAVTDQAPAAAPAPATAARPAPATVAADDDGP
                     PF"
     gene            complement(1578..2873)
                     /locus_tag="PKV30_09385"
     CDS             complement(1578..2873)
                     /locus_tag="PKV30_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRL30459.1"
                     /translation="MSDLGITFLLIAALFLLLGSGVWIGLTLTGVAWIGIQLFSSRPA
                     GDAMAVTIWGSASSWTLTALPLFVWMGEILFRTKLSESMFRGLAPWVTWLPGRLLHTN
                     VIGCAIFAAVSGSSAATCATIGKMTLPELGKRGYPEDITIGSLAGAGTLGLLIPPSII
                     MIVYGVAADVSIAKLFIAGVLPGILLAALFSGYLMVWALMNPGKVPRPDRVLSFSEKL
                     HESRHLIPVVLLIAAVLGSIYSGVATATEAAAVGVVGSLLLSLVQGSLSWDTFKTSLL
                     GATRLYCMIALILAGAAFLTLAMGYIGLPRHLAEFIGGLGLSPFMLMVALAVFYVVLG
                     CFLDGISMVVLTMGVILPTVQAAGFDLIWFGIFIVIVVEMAQITPPVGFNLFVLQGMT
                     KKDIAYIARVTMPFFLLMCAMVLMLWFFPGIATWMPGHM"
     gene            complement(2866..3381)
                     /locus_tag="PKV30_09390"
     CDS             complement(2866..3381)
                     /locus_tag="PKV30_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRL30460.1"
                     /translation="MRRFLNFLYDAAAWLAALAMIGVLVMVLTSIIGRLLHFHLPGTD
                     AYAGYSMAAAGFLALAHTLKKGEHIRVTLLIGRLQGSARRAMEMWSLSVAVLLAALFA
                     YYACRLVWQSHEFNDMSTSNDATPLWIPQLSMAVGAVILLIAFIDEWVLELLGQRSHE
                     GHADDEALHNE"
     gene            complement(3518..4498)
                     /locus_tag="PKV30_09395"
     CDS             complement(3518..4498)
                     /locus_tag="PKV30_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRL30461.1"
                     /translation="MKLTRRTLAVLTGALLGGLAASASAQTKWDLPSAYPASNFHTEN
                     LAQFAKDVQAATGGKLTIQVHDNASLFKAPEIKRAVQGGQAQAGEFLMVNFQNEWPLW
                     GLDGLPFLATSYAESFKLYQASKAAVQKKLGEQGMMLLYAVAWPPQGIFSKKQVNAAA
                     DMKGIKWRAYSPATARIGELVGAQPVTVQQAELSQAMATGVVESYMSSGSTGYDTKTY
                     EHLKFFADTQAWLPKNAVVANKKAFDALDKPTQDGLLKAAAEAEKRGWATSEKKTGEY
                     LDLLKKNGMTVYAPSAQLKSDMQKVGETMLKEWLDKAGPEGKAVVDAYRK"
     gene            4695..>4853
                     /locus_tag="PKV30_09400"
     CDS             4695..>4853
                     /locus_tag="PKV30_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRL30462.1"
                     /translation="MATKPPSRAAAQRAQGIVSSSHLVSPRSVEASEFEFGMIVAWNA
                     FSRWMVRCM"
BASE COUNT          861 a         1580 c         1590 g          822 t
ORIGIN      
        1 ccaggtcagc gccagcgagc cgagcaggtg cagcgcggcc gtcgacagcg ccgcgcccca
       61 gcggtcggcc agcgccatct cgaccacttc ggcgctgaac gacgagaagg tggtgagcgc
      121 gcccagaaag ccggtgacca ggatcagccg ccagaacggc gccaggtcgg catggtgcgc
      181 ccaggccaag atgaggccaa ccaggtagcc gccgatcagg ttggccgcca gcgtacccca
      241 gggcatgaag gcgccggggt ggttcagcca cagccccagc cgccagcgtg ccagcgcgcc
      301 gacgcaggcg cccaggcaga tggcgaacac ggcgtgcatg gcgcttcagc gggcaggggt
      361 tagaagggcg ggccgtcgtc gtcggcggcc acggtggccg gcgcggggcg cgcggcggtg
      421 gctggcgcgg gcgcggcggc gggggcttgg tcggtcacgg cggtcgcatc ggcctcgccg
      481 cccagcgcgt cgatcaggtc ggcgatcagg ccgctcaact cgcccgtgga cagggcgaca
      541 tcggcgtcga agcggtcgtc gtctttcgac gccgcgcctt cctcgaacac gccttcctga
      601 aaggcgattt ttttcagttg cagcgcctgc gtcagcatga aacccacgcg gccttgccaa
      661 cccagcgcca gcttggtggg cagcttgcct tcggcgatgt gctggcgcac ctcgtcggtg
      721 gccaggtcgt ggcgcgtgaa cttgacgacg gcgggctcgt cgccgctgcc tttcagttcg
      781 cattcgcgct cgacgttgaa ggcgtctgga atgtcctcgg ccgactcggc cgccagccac
      841 gccgccatcg cgccctgcgg cgattgctgg gtttgcagca ggccgacgcc aaaaccctgg
      901 ccggcaacgc gcaccaggct ggtgatgact tcatccgcct tgccttgcgc gcccacgtcc
      961 atcaccagcc agcgctgggt cggctcgatc cacacggtga cctggctgcg gcgcggaaag
     1021 gcttgcggca gcagggcgat cagtgcgtcg tccttcaggt cgcgcagctc tttcttgccg
     1081 ggcttgcggc cggtggtctt ttcgatttct tcaacgactt tttgcgttct ggcgcgcact
     1141 gcgtctgcgg ggacagcttt ggtttcgata gcaaagcggg cgatccactg gccatcgacc
     1201 gtctcgacca gcgcgccatg ctcctgcccg cgcggcggca cccagccggt ggatttttgt
     1261 tgcgtggcgc tgcattccac gaacggctcg ctggccagcg cttcttccca ttgcgccgcc
     1321 gaagtgggcc actcagggct caggcggtac agcatcaaat tcttgaacac aaacttgttt
     1381 cccaatgaaa tcaggcccgc gtggtgcgag cctgttggga atcatatcgg gctgtgacgg
     1441 aggctcaaac atccggacgc gaaggacgcg aagatagcgc gaaggacgca aaagaacagc
     1501 cagaaaattt ggtttttctt tttcgcgtcc ttcgcgaatc gtttgcgtcc ttcgcgtccg
     1561 gctgttttat ggcgccatca catatgcccc ggcatccacg tcgcgatgcc agggaagaac
     1621 cacaacatca acaccatcgc gcacatcaag aggaagaacg gcatggtcac gcgcgcgatg
     1681 taggcgatgt ctttcttggt catgccctgc agcacgaaca ggttgaagcc cacgggcggc
     1741 gtgatttgcg ccatttcgac cacgatgacg atgaagatgc cgaaccagat caaatcgaac
     1801 ccggcggcct gcaccgtggg caggatgacg cccatggtca gcaccaccat cgagatgccg
     1861 tccagaaagc agcccagcac cacgtagaac acggccagcg ccaccatcag catgaagggc
     1921 gacaggccca gcccgccgat gaattcggcc aggtgacgcg gcaggccgat gtagcccatg
     1981 gccagcgtga ggaaggccgc gccggcaagg atcagcgcga tcatgcaata cagccgcgtg
     2041 gcgcccagca gcgaggtctt gaaggtgtcc cacgacaggc tgccctgcac cagcgacagc
     2101 agcagcgagc cgaccacgcc cacggcagcg gcttcggtgg cggtggcgac gccggaatag
     2161 atggagccca gcacggccgc aatcagcagc accacgggga tcaggtggcg cgactcatgc
     2221 aacttttcgc tgaaggagag cacgcgatcc gggcgcggca ccttgcccgg gttcatcagc
     2281 gcccacacca tcaggtagcc cgagaacagc gccgccagca ggatgccagg cagcacgccg
     2341 gcaatgaaca gcttggcaat cgacacatcg gccgcgacgc cgtagacgat catgatgatc
     2401 gacggcggaa tcaacaggcc cagcgtgccg gcgccggcca gcgagccgat ggtgatgtcc
     2461 tcggggtagc cgcgcttgcc gagttccggc agcgtcatct tgccgatggt ggcgcaggtc
     2521 gcggccgacg agcccgacac ggcggcgaag atggcgcagc caatcacgtt ggtgtgcagc
     2581 aggcgcccgg gcagccaggt gacccacggc gccaggccgc ggaacatgct ttcgctcaac
     2641 ttggttctga acaggatctc gcccatccac acgaacaagg gcagggcggt cagcgtccag
     2701 cttgaggccg agccccagat ggtgaccgcc atcgcgtcgc ccgccgggcg cgacgagaac
     2761 agttggatgc cgatccacgc cacgccggtg agcgtgaggc cgatccagac gccgctgccc
     2821 agcagcagga acagcgccgc gatgagcagg aaggtgatgc cgaggtcact cattgtgcaa
     2881 agcctcgtcg tcggcgtggc cttcgtggct gcgctggccg agcagctcca gcacccattc
     2941 gtcgatgaag gcgatcagca aaatcaccgc gccgacggcc atggacagct gcggaatcca
     3001 cagcggcgtg gcgtcgttcg aggtcgacat gtcgttgaat tcgtgcgatt gccagaccag
     3061 ccggcacgcg tagtacgcga acagcgcggc cagcagcacg gccacgctga gcgaccacat
     3121 ctccattgcg cggcgcgcgc tgccctgcaa gcgaccgatc agcaatgtca cgcggatgtg
     3181 ctcgcctttt ttgagcgtgt gcgccagcgc cagaaagccc gccgccgcca tgctgtagcc
     3241 cgcgtaggcg tccgtgcccg gcaggtgaaa gtgcagcagg cgcccgatga tggacgtcag
     3301 caccatcacc agcacgccga tcatggccag tgccgccagc cacgcggcgg cgtcatagag
     3361 aaaattgagg aatcggcgca tggatcgaca gggttaagtt tccggtggcc gcggagcagg
     3421 ccgtaccagc gaacgccgtg gccggacctg tgccggccac tggcgttgtc ccccctgcgc
     3481 agcaaagggg ggaagccgcg tcagtggctc aggggggtca ctttctatac gcgtcgacaa
     3541 ccgccttgcc ctcaggcccg gccttgtcca gccattcctt cagcatggtc tcgccgacct
     3601 tctgcatgtc ggacttcaac tgggccgatg gcgcgtacac cgtcatgccg ttcttcttca
     3661 gcagatcgag gtactcgccg gtcttcttct cgctggtggc ccagccgcgc ttttcggctt
     3721 cggcggcggc tttcagcaag ccgtcctgcg tcggtttgtc gagggcgtca aaggccttct
     3781 tgttggccac cacggcgttc ttcggcagcc aggcctgcgt gtcggcaaag aacttcaggt
     3841 gttcgtaggt cttggtgtcg tagccggtcg agcctgagct catgtagctc tcgaccacgc
     3901 ccgtggccat ggcttgcgac agttcggcct gctgcaccgt gacgggctgc gcgccgacca
     3961 actcgccgat gcgcgcggtg gccgggctgt aggcgcgcca cttgatgccc ttcatgtcgg
     4021 cggcggcgtt cacttgcttc ttgctgaaga tgccctgcgg cggccacgcc accgcgtaca
     4081 gcagcatcat gccctgctcg cccagcttct tctgcacggc ggccttgctg gcctggtaga
     4141 gcttgaacga ctcggcgtag ctggtggcca ggaagggcag gccgtccagg ccccacagcg
     4201 gccattcgtt ctggaagttc accatcaaaa actcgcccgc ctgcgcttgc ccgccctgca
     4261 cggcgcgctt gatctcgggc gccttgaaga gcgaggcgtt gtcgtgcacc tggatggtga
     4321 gcttgccacc cgtggcggct tgcacatcct tggcgaactg cgccaggttc tcggtgtgaa
     4381 agttgctggc cgggtaggcg ctgggcaggt cccatttggt ctgcgcgctg gcgctggcgg
     4441 ccagcccgcc cagcaaggcg ccggtcaaaa cggccagggt gcgtcgggtc aacttcatgg
     4501 agactccttg agaagaatga tgaaacgtgg gaagcatagg ttgtgccatg ccgccgcgcg
     4561 aagggtaaaa accagaacgc ccggccgccg caccgcccat gccgtgccgt caaagcgctt
     4621 gaagtctagg gattgattcg ataaagtgtc aacaaattat ctgcaactta ccgagtgatt
     4681 tcgggtatac cctgatggcc accaagcccc cctcccgagc ggccgcgcag cgtgcgcagg
     4741 gcatcgtgtc gtcgtcgcac ctggtgtcgc cgcgcagcgt ggaggcgtcg gaattcgagt
     4801 tcggcatgat cgtggcgtgg aacgccttca gccgctggat ggtgcgctgc atg
//
LOCUS       DAOMVO010000189         4834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4961_length_4834_cov_15.333961, whole genome shotgun sequence.
ACCESSION   DAOMVO010000189 DAOMVO010000000
VERSION     DAOMVO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4961_length_4834_cov_15.333961"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PKV30_09405"
     CDS             complement(<1..553)
                     /locus_tag="PKV30_09405"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRL30463.1"
                     /translation="MRLRTLALLGAGGVALYRMAQQLGVPDGVVPVQPFQLSRYLGRW
                     YEIARIDHVYERGLTDTSADYRLLPGGEVQVTNRGYHPGARRWREVRARAQPVQGDDV
                     AHLQVSFMWPVRASYVVFGVDDDYQHALVSGPNHDYLWLLARTPQIRATARASMLDTA
                     RAAGFDVGRLMWVDQRRNVARALP"
     gene            736..1935
                     /locus_tag="PKV30_09410"
     CDS             736..1935
                     /locus_tag="PKV30_09410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL30464.1"
                     /translation="MTTHVVIIGGGAIGSAVARYLTRPEMQAAHPCRVTVLERDPSYA
                     RASSALSASSIRQQFSTAINLAISQYGITVLRDAARELAVPGEPPPDLGLHEGGYLYL
                     ATPAGEGVLRQNHAVQRAAGADVALLVPDQLAARFPWLATGDIALGSLGLSGEGWFDG
                     YSLLQAFRAQARVQGARYVVAEAVSFDIKKGHQRTSIQRIQLSSGEQIEADVVVNAAG
                     PWARQVAAWAGIELPVVAKRRTVFHVTCPEPLPHCPLLIDPSGIWLRPEGTGFITGFC
                     PPEADDGDDLPLDAPDHQAFDDFVWPTLAERIPAFEALRLQRTWAGYYEMNLFDHNAI
                     VGPHPACDNLIFANGFSGHGLQQSPAVGRGVAEWIAHGAYQTLDLSPLGWARVLADEP
                     LLERCVI"
     gene            complement(2162..2560)
                     /locus_tag="PKV30_09415"
     CDS             complement(2162..2560)
                     /locus_tag="PKV30_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30465.1"
                     /translation="MSPFDDLIADKGQPPLDKLTPLPADQLARLRAAHPGLPEGYLDF
                     LANVGFGELGDASFMLYGGVIEPDEAYGETPAGLQGVLLFGDDFAGTNCGFDSAGGWR
                     IVEIDPTNFSKDVVGEDFAAFIRIRIAAAA"
     gene            complement(2570..3730)
                     /locus_tag="PKV30_09420"
     CDS             complement(2570..3730)
                     /locus_tag="PKV30_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30466.1"
                     /translation="MSMIPFSPLGRAGVAAAQAGAHSLSPAARQALDGATTLGAVNPT
                     QLAQHIQRLPDAAARRQLLDEVAPHLPPRDLQDVQRELGQSPAKPQGIDADLALDLAQ
                     IGLDIAGIFDPTPISDGANTLISLGRSDWLGAGLSAVSMIPYVGDAAKLGKLGKYAQT
                     VAKAIDAARANPALRQALAPGIQAIGDAIAKLPLDKLPESARKQLTTLKELNGIFAKE
                     SDTAARTAWKAADDVPTTGAARAGAAASRVGAQPAWGKYLNDIKAQTGLAVSPKQQKL
                     IDEALAKNDYTRLSTAESKAHRAQFNKVKNELITQWEKQTGQSWPRYTEDLYSKNGTQ
                     VRRAGQPYDAHHIIESSYGGPNQWWNMHPARFPDQHQQGIHRAGGPSNQIFG"
     gene            3951..4190
                     /locus_tag="PKV30_09425"
     CDS             3951..4190
                     /locus_tag="PKV30_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRL30467.1"
                     /translation="MKNVTITVEDSVLEWARVEAARRNTSLSRMLGEFMAEMQRREDA
                     YERAYQEWRNDRRTWRSDGAPYPSRDEIHERGRWK"
     gene            4187..4642
                     /locus_tag="PKV30_09430"
     CDS             4187..4642
                     /locus_tag="PKV30_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL30468.1"
                     /translation="MTLVFVDTKVLIYAEDGAEAGKQHGALAWIERLWRARSGRLSTQ
                     VLNEFYVNVTRKLKPAMPQGDARARVRRYAAGWNPWQIDHATVETAWAMEARHGLQYW
                     DCLVLAAAQHSGCTLVLSEDMQHEGRYGAVQVINPFLASIDEVLGPDER"
     gene            4632..>4834
                     /locus_tag="PKV30_09435"
     CDS             4632..>4834
                     /locus_tag="PKV30_09435"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516475.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HRL30469.1"
                     /translation="MNAEIRKPLARIRQDVQSMHAYAVQDSTGMLKLDAMENPHRLSP
                     ELQAELGRRLGAVAINRYPGARV"
BASE COUNT          754 a         1577 c         1693 g          810 t
ORIGIN      
        1 gcggcagggc gcgcgcgacg ttgcggcgct ggtcgaccca catcagccgg ccgacgtcga
       61 agcccgccgc gcgcgcggtg tcgagcatgc tggcgcgcgc cgtggcgcgg atttgcggcg
      121 tgcgcgccag cagccacagg tagtcgtggt tggggccgct gaccagcgcg tgctggtaat
      181 cgtcgtcgac cccgaaaacc acgtaactcg cgcgcaccgg ccacatgaag ctgacctgca
      241 ggtgcgccac gtcgtcgccc tgcaccggct gggcgcgcgc ccgcacttcg cgccagcggc
      301 gggcgccggg gtggtagccg cggttggtga cctgcacttc gccgccgggc agcaggcggt
      361 agtcggcact ggtgtcggtc aggccgcgtt cgtacacgtg gtcgatgcga gcgatttcat
      421 accagcggcc cagatagcgc gacaactgaa acggctgcac cggcaccacg ccgtccggca
      481 cgcccagttg ctgtgccatt cggtacagcg ccacgccacc ggcgcccagc aaggcaagtg
      541 ttctcagtct catgacgggc gtcagcatag cggcggcgcc atgcgggggc tgtaggtgca
      601 ggccgcaaca tacccccggc ggggtagacc gtttgtaaga aaaaatggct gcggcgcatg
      661 tcggacaagc gctagcagct atcaaaacaa aagcacttga cggcaacggt gcgcgctcat
      721 tgcacaatgg cggccatgac aacacacgtg gtgatcatcg gcggcggcgc catcggcagc
      781 gccgtggcgc gctacctgac gcggcccgag atgcaggcgg cgcacccgtg ccgcgtgacg
      841 gtgctggagc gcgacccgag ctacgcgcgc gcctcgtccg cgctgtcggc atcgtccatc
      901 cggcagcagt tttcgaccgc catcaacctg gcgatctcgc agtacggcat caccgtgctg
      961 cgcgatgcgg cgcgcgaact ggccgtgccc ggcgagccgc cgcctgacct gggtctgcac
     1021 gaaggcggct acctgtacct ggccacgccg gcgggcgagg gcgtgctgcg ccagaaccac
     1081 gccgtgcagc gcgccgccgg tgccgacgtg gcactgctgg tgccggatca actggccgcg
     1141 cgctttccgt ggctggcgac cggcgacatc gcgctcggct cgctcggcct gtcgggcgag
     1201 ggctggttcg acggctacag cctgctgcag gcgtttcgcg cccaggcgcg ggtgcagggc
     1261 gcgcgctacg tggtggccga ggcggtgagt tttgatatca aaaaaggcca ccaacgcacg
     1321 tccatccagc gcatccagct atcgagcggt gagcaaatcg aggccgatgt ggtcgtcaac
     1381 gccgccggcc cgtgggcgcg ccaagtcgcg gcgtgggcgg gcatcgagct gccggtggtg
     1441 gccaagcggc gcacggtgtt tcacgtcacc tgcccggagc cgctgccgca ttgcccgctg
     1501 ctgatcgacc cttccggcat ctggctgcgg cctgaaggca ccggtttcat caccggcttt
     1561 tgcccgcccg aggcggatga cggcgacgat ctgccgctgg atgcacccga ccaccaggct
     1621 ttcgacgact tcgtctggcc gacgctggcc gagcgcattc cggccttcga ggcgctgcgc
     1681 ctgcagcgca cctgggccgg ttattacgag atgaacctgt tcgaccacaa cgccatcgtc
     1741 gggccgcatc cggcgtgcga caacctgatt tttgccaacg gcttttccgg ccacggcctg
     1801 cagcaatcgc ccgccgtggg gcgcggcgtg gccgagtgga ttgcgcacgg ggcgtaccag
     1861 acgctggact tgtcgccgct cggctgggcg cgcgtgctgg ccgatgagcc gctgctggag
     1921 cgctgcgtga tctgacgccg ttcgctatat taaaaatagc tgattgcgct tgtccatcaa
     1981 gcgctggcgg gcatttcgat ggcgaaatga cttcgcttcg ctgcaatgac gaaatgaccg
     2041 cgggcgtggg gtcatggcgc gttgcgccgt cgtcgacggc ccacggccga ggtcatgcgc
     2101 cgcaggcgcg tcatcaaaat gcacagctgc tcgcgctggc ccagcaggcg ctgcagcagg
     2161 atcaggccgc cgccgcgatg cggatgcgga tgaaggcggc aaagtcttcg cccaccacgt
     2221 ccttgctgaa gttggtcggg tcgatttcga cgatgcgcca gccgcccgcg ctgtcaaacc
     2281 cgcaattggt gccggcaaag tcgtcgccga acagcagcac gccttgcagg ccggcgggcg
     2341 tctcgccata ggcttcgtcc ggctcgatca ccccgccgta gagcatgaac gacgcatcgc
     2401 ccagctcgcc aaaaccaacg ttggccagaa agtcgaggta gccctcgggc aagcccgggt
     2461 gcgccgcgcg caggcgcgcc agctggtcgg ctggcagtgg cgtgagtttg tccagcggcg
     2521 gctggccttt gtcggcaatg aggtcgtcga atgggctcat gggatggggt caaccgaaga
     2581 tctggttgct ggggccgcca gcgcggtgaa tgccctgctg gtgctggtcg ggaaagcgcg
     2641 ccgggtgcat gttccaccac tggttggggc cgccgtagct gctttcgatg atgtggtgcg
     2701 catcataagg ttggcccgca cggcgcacct gcgtgccgtt ctttgaatac aggtcttcgg
     2761 tgtagcgcgg ccagctttgg ccggtctgct tttcccattg tgtgatcagc tcgttcttga
     2821 ccttgttgaa ctgcgcgcgg tgcgccttgg attcggcggt ggacaggcgc gtgtagtcgt
     2881 tcttggcgag tgcttcgtcg atcagctttt gctgtttggg gctgacggcc aggccggtct
     2941 gggccttgat gtcgttcagg tacttgcccc aggccggctg cgcgccaacg cggctggcgg
     3001 cagcgcctgc acgcgccgcg ccggtggtag gcacgtcgtc cgcagccttc cacgcggtgc
     3061 gcgcggcggt gtcggattct ttggcgaaga tgccgttcag ctctttcagc gtggtgagct
     3121 gcttgcgggc ggactcgggc agtttgtcca gcggcagctt ggcgatggcg tcgccaatcg
     3181 cctggatgcc gggcgccagc gcctggcgca gcgccgggtt ggcgcgcgcg gcgtcgatcg
     3241 ccttggccac cgtctgcgcg tacttgccga gcttgcccag cttggctgcg tcgcctacgt
     3301 agggaatcat gctgacggcg ctcaaacccg cgcccagcca gtcgctgcgg ccgagcgaaa
     3361 tcagcgtgtt ggcgccgtcg gagatgggcg tggggtcgaa gatgccggcg atgtcgaggc
     3421 cgatttgcgc cagatccagc gcgagatcgg cgtcgattcc ttggggcttg gcgggcgact
     3481 ggcccagctc gcgctgcacg tcttgcagat cgcgcggcgg caggtgcggc gccacttcat
     3541 ccagcagctg gcggcgcgcg gcggcgtcgg gcaggcgctg gatgtgctgc gccagttgcg
     3601 tcgggttgac ggcgcccagc gtggtggcgc catccagcgc ctgacgggcg gcgggcgaca
     3661 ggctgtgcgc gccggcctgc gcggcggcga caccggcgcg acccagcggc gaaaaaggga
     3721 tcatcgacat ggcgggattt ccatccagcc ccgtggcatg gggcgatgcg cctattgcag
     3781 cgcagcacga cggcgccgcc cagtcggcgc gttaccaggc tgggagcgac accaacgggg
     3841 gatcgatgcc catgtttcgg tcatgcgctc gtcataccag acgggtaacg cggccgaagt
     3901 ttgttaacac gaatttcgtg caacacgaaa atcgtgttat gctgcgccgc atgaaaaacg
     3961 tcaccatcac cgtcgaagac agcgtgctgg aatgggcgcg cgtcgaggcg gcgcgccgca
     4021 acaccagcct gtcgcgcatg ctgggtgagt tcatggctga aatgcagcgc cgtgaggacg
     4081 cttatgaacg cgcctaccag gagtggcgca atgaccgacg cacctggcgc tcggacggcg
     4141 cgccgtaccc ctcgcgcgac gaaattcacg agcgcgggcg ctggaagtga cgctggtatt
     4201 cgtcgatacc aaggtgctga tctacgccga agacggcgca gaggcgggca agcagcacgg
     4261 cgcgctggcc tggatcgagc ggctgtggcg cgcgcgcagc ggccgcctgt ccacgcaggt
     4321 gctgaacgag ttctacgtca acgtcacgcg caagctcaaa ccagccatgc cgcaaggcga
     4381 tgcgcgggcc cgcgtgcgcc gctacgccgc tggctggaac ccctggcaga tcgaccacgc
     4441 cacggtggaa accgcctggg ccatggaggc gcgacatggc ctgcaatact gggactgcct
     4501 ggtgctggcc gccgcccagc acagcggctg cacgctggtg ctctctgaag acatgcagca
     4561 cgaaggccgc tatggcgccg tgcaagtgat caaccctttt ctggcctcga tcgacgaggt
     4621 gctgggcccc gatgaacgct gaaattcgca aacccctggc ccgcatccgc caggacgtgc
     4681 aatccatgca cgcctacgcc gtgcaggatt cgaccggcat gctcaagctc gatgccatgg
     4741 agaacccgca tcgcctgtcg cccgagttgc aggcggagct gggccgccgg ctgggcgcgg
     4801 tcgccatcaa ccgctacccc ggcgcgcggg tcga
//
LOCUS       DAOMVO010000190         4825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_4974_length_4825_cov_18.028092, whole genome shotgun sequence.
ACCESSION   DAOMVO010000190 DAOMVO010000000
VERSION     DAOMVO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4974_length_4825_cov_18.028092"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKV30_09440"
     CDS             <1..861
                     /locus_tag="PKV30_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30470.1"
                     /translation="VSKLMTRLETRLGVQLLHRSTRKVQLKPEGWRLYEQGQRVLADL
                     DELERSVATAAEPRGTVRITASTSTGHMLLVPLVPRLLAEYPGLSLDLRFTDEVVDLA
                     DAGIDIAVRWGQLPASDLIARRLGQTRQAIVAAPAYLAAHGCPQQPDELQAHIRLGWN
                     YRRAIPAWPFQVDGRRVEVDIGDVVRVNDGDVMRQLAIAGAGLARLSVYHAWHDLAAG
                     RLVPVLEAFNTGELEPIHAVYLGRPDRLPARTRVVLDFLQAHVDLRHAERALPTSPHE
                     WQDAAHLSNA"
     gene            877..1245
                     /locus_tag="PKV30_09445"
     CDS             877..1245
                     /locus_tag="PKV30_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRL30471.1"
                     /translation="MNALRGLAWLLVLQLVGEVLARAFSLPLPGPVIGMLLLMPALGW
                     RPLREPVTEVARFLLAHLSLLFVPVGVGVITHLDVLRQHGFGLLAVIALSTWIGLAVS
                     ALTLRALIRSRADEAEAPDA"
     gene            1238..1963
                     /locus_tag="PKV30_09450"
     CDS             1238..1963
                     /locus_tag="PKV30_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRL30472.1"
                     /translation="MPDFVQLWVYLSSAPLFGLTATLVVYLLAQRFYAAMDQAPWANP
                     VLWSVLSLGAGLLATGVPYPSYFAGAQFIHFLLGPAVVALGFPLWERRAQLRRDWGRF
                     VAAALAGGAAAAISAVALGWAFGLPDDVLRSLAPKSVTAPVAMGVADKIGGVPALAAV
                     FAVLTGLIGALSGKYLFAMIGVGTDRVGWMARGFAMGTAAHGIGAARSLQVNAEAGAY
                     AGLALGLQVLLASVLMPLAARWF"
     gene            complement(1965..2696)
                     /locus_tag="PKV30_09455"
     CDS             complement(1965..2696)
                     /locus_tag="PKV30_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRL30473.1"
                     /translation="MTARRPIHPTVFERPHHQRIASVLQGLDGELLRTHACSFGGGTA
                     IALRYGELCESVGIDFLMSDLAHYRQLRLLATGPSGFAALTRGGVAPWAAAQPVRADQ
                     YGIRGQVVVVGQPITLEIVLEARIALQPARTADDVCGVPSLTALDLAASKLLANADRW
                     LEDVVFSRDVIDLAMMRPRLPLLREALSKAERAYGPAVRRDLGRAIDRLAERTGWLER
                     CMQAMAIRVPKALLWQRIRALRRVL"
     gene            complement(2659..3159)
                     /locus_tag="PKV30_09460"
     CDS             complement(2659..3159)
                     /locus_tag="PKV30_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL30474.1"
                     /translation="MPTAAPALTSSAQALTQALGAAVRAQRKGLRVSATALAEAAGVS
                     RVTVYRIEKGEPSVTLGAYANVLTALGLGLKALPESADATSAAPLGWLPARVRLADYP
                     QLRALAWHVPGAEALSPAQAWGLYQRNARHLDAAALTPAEQQLIQALQQAFADDGAPP
                     HSPDGV"
     gene            complement(3233..3568)
                     /gene="cutA"
                     /locus_tag="PKV30_09465"
     CDS             complement(3233..3568)
                     /gene="cutA"
                     /locus_tag="PKV30_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRL30475.1"
                     /translation="MTQSLLLVLTTVATRADADALARAMVEQRLAACAQISAIDSVYR
                     WQGAVQSEGEFRVLFKTTAERYPALEAALRSSHPYELPAIVALPVVQALPAFADWVIT
                     EVNCPVVIE"
     gene            complement(3591..4757)
                     /locus_tag="PKV30_09470"
     CDS             complement(3591..4757)
                     /locus_tag="PKV30_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL30476.1"
                     /translation="MPGLLPNIDPDGLLEFSVVYTDRALNHMSKKFQGVMKDIAAMLK
                     EVYHADAAVLVPGSGTFGMESVARQFAGDQRVVIIRNGFFSFRWSQILETGRITAPEN
                     ITVLKARRTGGDAQSPWAPAPIDEVVSTIASVKPAVVFAPHVETASGMMLPDDYMKKI
                     ADAVHAVGGLFVLDCIASGAMWVDMQAIGVDVLVSAPQKGWSSSPACAMVCLSDRALK
                     TLEGTTSTSYACDLKKWHQIMQAYETGSHAYHATMPTMALLKLRDTMQETRERGFAKV
                     REQQIELGRKVRALMEGRGLPSVAAEGFKAPGVVVSYTSDPEIQNGKAFIAQGVQSAA
                     GVPLMCDEPADFRTFRLGLFGLDKWANVDRSVGHLAAALDRMGVTAPEKVAVSA"
BASE COUNT          712 a         1642 c         1642 g          829 t
ORIGIN      
        1 gtcagcaagc tgatgacacg gctggaaacg cgcctgggcg tgcagctttt gcaccgcagc
       61 acgcggaaag tgcagctcaa gcccgagggc tggcgcctgt acgagcaggg ccagcgcgtg
      121 ctggccgacc tggacgagct ggaacgctcg gtcgccaccg ccgccgaacc gcgcggcacg
      181 gtgcgcatca ccgccagcac ctcgaccggc cacatgctgc tggtgccgct ggtgccgcgc
      241 ctgctggctg agtaccccgg cttgagcctg gatttgcgct ttaccgatga agtggtcgat
      301 ctggccgacg ccggcatcga catcgccgtg cgctggggtc agctgccggc gtcggacctg
      361 atcgcgcgcc ggctggggca gacgcgccag gccatcgtcg ccgcgcccgc ctacctggcc
      421 gcgcacggct gcccgcagca accggacgaa ctacaagccc acatccgcct cggctggaac
      481 taccgccgcg ccatcccggc ctggccgttt caggtcgatg gccggcgggt cgaagtcgac
      541 attggcgacg tggtgcgcgt gaacgatggc gacgtcatgc gccagttggc catcgccggc
      601 gccggtctgg cgcgtttgtc ggtctaccac gcgtggcacg acctggccgc cgggcggctg
      661 gtgccggtgc tggaagcctt caacaccggc gagctggagc ccatccacgc cgtctacctg
      721 ggccgccccg accgcttgcc ggcccgcacg cgcgttgtgc tggatttttt gcaggcgcac
      781 gttgatctgc gccacgccga gcgggcgctg cccacatcgc cccacgaatg gcaagatgcc
      841 gcccatctgt ccaatgcctg ataaaaatcc tgtccgatga acgccctgcg aggtcttgcc
      901 tggctgctgg tgctgcaact ggtcggcgaa gtgctggcgc gcgccttcag cctgccgctg
      961 cccggcccgg tgatcggcat gctgctgctg atgccggcgc tcggctggcg cccgctgcgc
     1021 gagccggtga ccgaggtggc gcgctttctg ctggcgcacc tgtcgctgct gttcgtgccg
     1081 gtgggcgtgg gcgtcatcac gcacctggat gtgctgcggc aacacggctt cgggctgctg
     1141 gcggtgatcg cgctgtcgac ctggatcggc ctggcggtga gcgcgctcac cctgcgcgcg
     1201 ctgatccgct cgcgcgccga cgaagcggag gcgcccgatg cctgacttcg ttcagttgtg
     1261 ggtctacctg tcgtccgccc cgctgttcgg gctgacggcg acgctggtgg tgtatctgct
     1321 ggcgcagcgc ttttacgccg ccatggacca ggcgccctgg gccaacccgg tgctgtggtc
     1381 ggtgctgtcg ctgggcgccg ggctgctggc caccggcgtg ccgtacccga gctactttgc
     1441 cggcgcgcag ttcatccact ttctgctcgg acccgcggtg gtggcgctgg gctttccatt
     1501 gtgggagcgg cgcgcgcaac tgcgccgcga ttgggggcgt tttgtggccg cagcgctggc
     1561 ggggggcgcg gcagcagcta tcagtgctgt agcactcggc tgggcgtttg gcttgcccga
     1621 cgacgtgctg cgctcgctgg cgcccaagtc ggtcaccgcg ccggtcgcca tgggcgtggc
     1681 cgacaagatc ggcggcgtgc cggcgctggc ggcggtgttc gcggtgctga cggggctgat
     1741 cggcgcgctg tcgggcaagt acctgtttgc gatgatcggc gtgggcaccg accgcgtcgg
     1801 ctggatggcg cgcggcttcg ccatgggcac cgccgcgcac ggcatcggcg ccgcgcgctc
     1861 gctgcaggtg aatgccgaag ccggcgccta cgccgggctg gcgctgggcc tgcaggtgct
     1921 gctggcgtcg gtgctgatgc cgctggcggc gcggtggttt tgactcacag cacgcgccgc
     1981 agcgcgcgga tgcgctgcca cagcagcgcc ttgggcacgc ggatcgccat ggcctgcatg
     2041 cagcgctcca gccagcccgt gcgttcagcc agccggtcga tcgcccggcc cagatcgcgc
     2101 cgcaccgccg gaccataggc tcgctctgct tttgaaagcg cctcgcgcag caagggcaaa
     2161 cgcgggcgca tcatggcgag gtcaatcacg tcgcggctga acaccacgtc ttccagccag
     2221 cggtcggcat tggcgagcag cttgctggcc gccagatcga gcgcggttag cgaaggcacg
     2281 ccgcacacgt cgtcggccgt gcgggcgggt tgcagggcga tgcgtgcctc cagcacgatt
     2341 tcgagtgtga tcggctggcc caccacgacc acctggccgc gaatgccgta ttggtcggcc
     2401 cgcaccggct gcgcggcagc ccagggcgcc acgccgccgc gcgtcagcgc cgcgaagccg
     2461 ctcggcccgg tggccagcaa gcgcaactgg cggtagtgcg ccagatcgga catcaaaaag
     2521 tcgatgccca ccgattcaca gagttcgccg tagcgcagcg caatggccgt gccgccgccg
     2581 aagctgcatg cgtgcgtgcg cagcagctcg ccatccagcc cctgcagcac gctggcgatg
     2641 cgctggtggt gcgggcgctc aaacaccgtc gggtgaatgg ggcggcgcgc cgtcatcggc
     2701 aaacgcctgt tgcagcgcct ggatcagttg ctgctctgca ggggtcagcg cggcggcgtc
     2761 caggtggcgg gcgttgcgct ggtacaggcc ccaggcttgc gcggggctca aggcttcggc
     2821 gcccggcacg tgccaggcca gcgcgcgcaa ctgcgggtag tcggccaggc gcacgcgggc
     2881 gggcagccag ccgagcggcg ctgcgctggt tgcatcagcg ctttcgggca atgccttcaa
     2941 acccagcccc aacgccgtca acacattggc gtaagcgccc agcgtgacgg agggctcgcc
     3001 tttttcgatg cggtacacgg tcacgcgcga cacgcccgcc gcctcggcca gcgcggtggc
     3061 gctcacgcgc aagcccttgc gctgcgcgcg cacggcggcg cccagcgctt gcgtcagcgc
     3121 ctgggcgctt gatgtcagcg cgggggcggc ggttggcatg tgtttctcat tttcgtcttt
     3181 ttgatgaaaa agtcaaaaat gagaaacgtt ttctgaattg cagtttgatg gcctattcaa
     3241 taacgacagg acagttcacc tcagtgatga cccaatcggc aaacgccggc aaagcctgca
     3301 ccaccggcag cgcgacgatg gcaggcagct cgtacgggtg acttgaacgc agcgcggctt
     3361 ccagcgccgg atagcgctca gccgtggtct tgaacaacac gcggaattcg ccctcggact
     3421 gcaccgcgcc ttgccaccga tacacgctgt caatcgcact gatctgcgcg cacgcggcca
     3481 ggcgctgctc gaccatcgca cgcgccagcg catccgcatc ggcgcgcgtg gcgacggtgg
     3541 tcagtaccag cagcaggctt tgcgtcaaat cagcccctgg cgcccgtcca tcaagcgctg
     3601 acagctactt tttcaggagc agtcacaccc attcgatcca gcgccgccgc gaggtggcca
     3661 acgctgcggt ccacgttggc ccacttgtcg aggccgaaca ggccgaggcg gaaggtgcgg
     3721 aagtcggctg gctcatcgca catcagcggc acgccggccg cgctttgcac gccttgggcg
     3781 atgaaagcct tgccgttctg gatttccggg tcggaggtgt agctgaccac cacgcccggc
     3841 gccttgaagc cttcggccgc cacgctgggc aggccgcgcc cttccatcag cgcgcgcacc
     3901 ttgcggccca gttcgatctg ctgctcgcgc accttggcaa agccgcgctc gcgcgtctct
     3961 tgcatggtgt cgcgcagctt gagcagtgcc atggtcggca tggtggcgtg gtaggcgtgg
     4021 ctgccggttt cgtaagcctg catgatctgg tgccacttct tcaagtcgca ggcgtagctg
     4081 gtgctggtcg tgccttccag cgttttcagc gcgcgatccg acaggcagac catcgcgcag
     4141 gcgggcgacg agctccagcc cttttgcggc gcgctgacca gcacatcgac accgatggcc
     4201 tgcatgtcca cccacatcgc gcccgaggca atgcagtcca gcacgaacag gccgcccacc
     4261 gcgtgtacgg cgtcggcgat ctttttcatg tagtcatccg gcagcatcat gccgctcgcg
     4321 gtttcgacgt gcggtgcgaa gacgaccgcc ggtttcacgc tggcgatggt cgacaccacc
     4381 tcgtcaatcg gcgccggcgc ccagggcgat tgcgcatcgc cgccggtccg gcgcgccttc
     4441 agcacggtga tgttctcggg cgcggtgatg cggccggttt ccagaatctg gctccagcga
     4501 aagctgaaaa aaccgttgcg gataatcacc acccgctggt cgcccgcaaa ctggcgcgcc
     4561 accgactcca tgccaaaggt gccgctgccg ggcaccagca cggcggcgtc ggcgtggtac
     4621 acctctttca gcatcgcggc gatgtctttc atcacgccct gaaacttctt cgacatgtgg
     4681 ttcaaggcgc ggtcggtgta gaccaccgag aattcgagca aaccgtcggg atcgatgttc
     4741 ggcagcagtc cgggcatggg gttctccttg acaaaggtcg cggccagcac ggcgccgacc
     4801 tgaaaaaatt cactttacac gcaag
//
LOCUS       DAOMVO010000191         4753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5087_length_4753_cov_13.242231, whole genome shotgun sequence.
ACCESSION   DAOMVO010000191 DAOMVO010000000
VERSION     DAOMVO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5087_length_4753_cov_13.242231"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1400)
                     /locus_tag="PKV30_09475"
     CDS             complement(3..1400)
                     /locus_tag="PKV30_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HRL30477.1"
                     /translation="MIDPLRIAIVGHTNAGKTSLLRTLTRNVDFGEISDRPGTTRHAE
                     TIDLRLDGQVAVRFIDTPGLEDSVALLDYLSTLPAGSRPERVRAFLRGPEASRSFEQE
                     AKVLRALIEGADAAMIVIDTREPVLPKYRAELEILSWCAKPIMPVLNFVRYVGTRLDD
                     WHQALLESGLHARVEFDVVAPFNGSERQLYSDLATLLPARRQQLNAIVDALALQARER
                     RLAACRVIASGLIDVAALRRALPADEFADELRQKAFVRSFQDDVRRHARRAVDDLLAV
                     FAFRAGDAELADLPQLGGRWEDDLFNPELLKEAGKRLGMGAMIGAGLGAVADVAMAGL
                     SLGAATTLGATIGGAASGGWRPLWRKLENRLNGVQELTVEDPVLLLLADHLLDLARAL
                     EQRGHAAQDKLRLDDERIDEDGLRPVVSALGAARGHPDWERGEGRRGAGGSDRQALEA
                     EIARELDRRFDSPVA"
     gene            complement(1393..2802)
                     /locus_tag="PKV30_09480"
     CDS             complement(1393..2802)
                     /locus_tag="PKV30_09480"
                     /inference="COORDINATES: protein motif:HMM:NF022518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HRL30478.1"
                     /translation="MTPSNPDTPRSPLATALMLDAVQRIEADGPLDDQPALRQAFATQ
                     PTRAAQVQERAWLLGQRLDLPHEWARWRQMGWLAVAALALLMAFTGLGLARAVLGEGR
                     SINAVAAFVSLLGLHAVMLLIWFVALALSHGRWAPPLGRAALWLTARLPFERGPHAPV
                     LLQSFTAVLQRQRLLGWLTGAISHGIWTLAFVITLAVLAFGFAFHAYTLTWETTILSA
                     DFFQRFVQITGALPALLGFAVPDAAAVQRVGNAAVSANQREWAWWLMGCVVVYGLLPR
                     ALLAALCLWRWRAGTRRLQGQVDMADPYVLRLVQRLDALEPPPEVIDPERPAPFRAPP
                     KRSSLPPGAPGSLAVIGFELPPEVPWPLPDLPESTEPPQRIAGSANERQAVLAQLAQA
                     RPESLLLVVHAASSPDRGTARFVREAMREAGRTALLLNGNEGGARRWRDWMVSEGFEA
                     LALAESAQAATDWIANRHD"
     gene            2957..3898
                     /locus_tag="PKV30_09485"
     CDS             2957..3898
                     /locus_tag="PKV30_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL30479.1"
                     /translation="MSLDSAAPATWWARLGQRPALLLILTTLIWGSNAVAARLAVGQV
                     SPMMLTTARWSIACVALWVVARRQVAAHWSALRPRWAYFAVMGSCGFTAFNALFYTAA
                     HHTTAVNLALIQGVIPVLVLLGGVLFLRLRVTPLQMLGVAVTLAGVAVVAARGQWQAL
                     AALDFNVGDVAIMLASLLYAGYTLGLRSRPAVPPLVFFAAVAAVAALVSLPLLAWEVW
                     AGRFFWPTPLGWLLLLYVGLMPSFVSQIMFIRGVELIGPARASVFLNLNPVFGPALAV
                     LLLGEPLKPYHLLALAMVLGGIWIAERLGARPSLQNK"
     gene            complement(3940..4464)
                     /gene="ppa"
                     /locus_tag="PKV30_09490"
     CDS             complement(3940..4464)
                     /gene="ppa"
                     /locus_tag="PKV30_09490"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRL30480.1"
                     /translation="MSLDNVTPGNKAPDEFNVVIEIPMNADPIKYEVDKETSAIFVDR
                     FMSTAMHYPTNYGYVPKTLSGDGDPVDVLVITPVPLIPGVVVTCRPIGILKMQDEAGE
                     DGKVLAVPIPKILSLYNWEKPEDLSPIRLKTIAHFFEHYKDLEPGKWVKVLGWEGPEA
                     ARKEIMDGIAAYKK"
BASE COUNT          690 a         1612 c         1664 g          787 t
ORIGIN      
        1 cctcacgcca cggggctgtc gaagcgccga tccagctcgc gcgcgatctc ggcttccagc
       61 gcctgccggt cgctgccgcc cgcaccgcgc cggccttcgc cgcgctccca gtcggggtgg
      121 ccgcgcgcgg cgccgagcgc gctgaccacc gggcgcaggc cgtcttcgtc gatgcgctcg
      181 tcatccaggc gcagcttgtc ttgcgcggcg tggccgcgct gctccagcgc gcgggccagg
      241 tcgagcaggt gatcggccag caacaggagc accgggtctt ccaccgtcag ctcctgcacg
      301 ccgttcaggc ggttctccag cttgcgccac agcgggcgcc agccgccgga cgcggcgccg
      361 ccaatcgtgg cgccgagcgt cgtggcggcg ccgagcgaca ggcctgccat cgccacgtcg
      421 gccacggcgc cgaggccggc gccgatcatg gcgcccattc cgaggcgctt gccggcttcc
      481 ttgagcagct cgggattgaa caaatcgtct tcccagcggc cgcccagttg cggcagatcg
      541 gccagttcgg cgtcgccagc gcgaaaggcg aacacggcca gcaaatcgtc gaccgcgcgc
      601 cgcgcgtggc gccgcacatc gtcctgaaag ctgcgcacaa aagccttctg gcgcaattcg
      661 tcggcaaact cgtcggccgg cagcgcgcgg cgcagggcgg cgacgtcgat caggccgctg
      721 gcgatgacgc ggcaggccgc caggcgccgc tcgcgcgcct gtagcgccag cgcatcgacg
      781 atggcattca actgctggcg gcgcgcgggc aataacgtgg ccaggtcact gtagagctgg
      841 cgctcggagc cgttgaaggg cgccaccaca tcgaattcaa cccgcgcgtg caggccgctc
      901 tccagcagcg cctggtgcca gtcgtccagc cgcgtgccca cgtagcgcac gaagttcagc
      961 accggcatga tgggcttggc gcaccagctc aaaatctcca gctcggcgcg gtatttgggc
     1021 aggaccggct cgcgcgtgtc aatgacgatc atggcggcgt cggcgccttc gatcagcgcg
     1081 cgcaacacct tggcttcttg ctcgaagctg cggctggcct cgggcccgcg caggaaggcg
     1141 cgcacgcgct cggggcgcga accggcgggc agcgtgctca ggtagtcgag cagcgcgacc
     1201 gaatcttcca ggccaggcgt gtcaatgaag cgcacggcga cctggccgtc cagccgcaaa
     1261 tcgatggtct cggcgtgccg cgtggtgccg gggcggtcgg agatttcccc aaagtcaacg
     1321 ttgcgcgtca gcgtgcgcaa gagcgaggtc ttgccggcgt tggtgtggcc gacgatggcg
     1381 atgcgaagcg ggtcaatcat ggcgattcgc tatccaatca gtagctgctt gcgctgattc
     1441 agcaagcgct agcgcctcaa aaccttctga aaccatccag tcgcgccagc gccgcgcgcc
     1501 gccctcgttc ccgttcagca gcagcgcggt gcggccggct tcgcgcatcg cctcgcgcac
     1561 gaagcgggcg gtgccacgat ccgggctgga ggcagcgtgc accaccagca gcagcgattc
     1621 ggggcgtgct tgggcgagct gtgccagcac ggcctggcgt tcgttggcgc tgccggcgat
     1681 gcgctgcggc ggctcggtgc tttcgggcaa atcgggcagc ggccagggca cctcgggcgg
     1741 cagttcgaag ccgatcaccg ccagcgaacc cggcgcgccg ggcggcaggc tggatcgctt
     1801 gggcggggcc ctgaagggcg cggggcgctc ggggtcgatg acttcgggcg gcggctccag
     1861 tgcatcgagg cgctgcacca ggcgcagcac gtaagggtcg gccatgtcga cctgcccttg
     1921 cagccgccgc gtgccggcgc gccagcgcca caaacacagc gccgccagca gggcgcgcgg
     1981 cagcagcccg tagaccacca cgcagcccat cagccaccac gcccattcgc gctgattggc
     2041 cgacaccgcg gcgttgccca cgcgctgcac ggcggcggcg tcgggcacgg caaagcccag
     2101 cagcgcgggc agcgcgccgg tgatctgcac gaagcgctgg aaaaaatcgg cgctcaagat
     2161 cgtcgtctcc cacgtcagcg tgtaggcgtg aaaggcaaag ccaaacgcca gcacggccag
     2221 cgtgatgaca aacgccaggg tccagatgcc gtggctgatg gcccccgtga gccagcccag
     2281 caggcgctgg cgctgcagca cggcggtgaa ggactgcagc agcaccggcg cgtgcgggcc
     2341 gcgttcgaaa ggaaggcgcg cggtgagcca cagcgccgcg cgccccagcg gcggcgccca
     2401 gcgtccgtgc gacagcgcca gcgcgacaaa ccagatcaac agcattaccg cgtgcagccc
     2461 cagcaagctg acaaacgccg ccacggcgtt gatgctgcgc ccctcgccca gcacggcgcg
     2521 cgccaggccg aggccggtga aggccatcag cagcgccagc gccgccacgg ccagccagcc
     2581 catctggcgc cagcgcgccc attcatgcgg caagtccagc cgctggccca gcagccaggc
     2641 gcgctcttgc acctgcgcgg cgcgcgtcgg ttgggtggca aaggcctggc gcagcgccgg
     2701 ctggtcgtcg agcgggccat cggcttcgat gcgctgcacg gcatccagca tcagcgcggt
     2761 ggccaggggg ctgcgtgggg tgtcggggtt cgaaggcgtc atggcggcta gcgcgggatt
     2821 atcgggcgac cctgcgagcg gcttccatgg agcggttcgt gattcacggc gcgccgtcgg
     2881 ccctcacccc tgccctctcc cagggagagg gggtgtcatt aaacaggcac gcgccaaccg
     2941 ggttaccctc gccgccatgt ctttggattc cgctgcgcct gccacgtggt gggcgcggct
     3001 tggccagcgc cccgcgctgc tgctgatcct caccacgctg atctggggca gcaacgccgt
     3061 ggccgcgcgg ctggcggtgg gccaggtgtc gccgatgatg ctgaccaccg cgcgctggag
     3121 catcgcctgc gtcgcgcttt gggtggtggc gcgccgccag gtggcggcgc actggtcggc
     3181 gctgcggccg cgctgggcct actttgccgt catgggctcg tgcggcttca ccgccttcaa
     3241 cgccctcttc tacaccgccg cgcaccacac caccgccgtg aacctggcgc tgatccaggg
     3301 cgtgattccg gtgctggtgc tgctgggcgg cgtgttgttt ctgcgcctgc gcgtgacgcc
     3361 gctgcagatg ctgggcgtgg cggtcacgct ggccggcgtg gcggtggtgg ccgcgcgcgg
     3421 gcagtggcag gcgctggcgg cgctggactt caacgtgggc gacgtggcca tcatgctggc
     3481 gtcgctgctg tacgccggct acacgctggg cctgcgcagc cggccggccg tgccgccgct
     3541 ggtgtttttt gccgccgtgg cggcggtggc ggcgctggtg tcgctgccgc tgctggcgtg
     3601 ggaagtgtgg gccgggcgct ttttctggcc cacgccgctg ggttggctgc tgctgctgta
     3661 cgtggggctg atgccttcgt tcgtgtcgca gatcatgttc atccgcggcg tggaactgat
     3721 cggcccggcg cgcgccagcg tgtttttgaa cctgaacccc gtgttcgggc cggcgctggc
     3781 ggtgctgctg ctgggcgagc cgctcaagcc ctaccacctg ctggcgctgg cgatggtgct
     3841 gggcggcatc tggatcgccg agcgcctggg cgcccgacct tcgctacaaa acaaatagct
     3901 tctcgcgctt gttggacggg cgctggaggc ctttttggct tattttttat aggctgcgat
     3961 gccgtccatg atttccttgc gggcggcctc aggcccttcc cagcccagca ccttgaccca
     4021 cttgcccggc tccagatcct tgtagtgctc gaagaagtgg gcgatggtct tgaggcggat
     4081 cgggctcagg tcttccggct tttcccagtt gtagagcgac aaaatcttgg ggatcggcac
     4141 cgccagcacc ttgccgtctt cgcccgcctc gtcctgcatc ttcaagatgc cgatcggccg
     4201 gcaggtaacc accacgcccg gaatcagcgg caccggcgtg atgaccagca catcgaccgg
     4261 atcgccgtcg cccgacagcg tcttgggcac gtagccgtag ttggtggggt aatgcatcgc
     4321 cgtgctcatg aagcggtcga cgaagatggc gctggtttcc ttgtcgacct cgtacttgat
     4381 cgggtcggcg ttcatcggga tttcgatgac gacgttgaac tcgtcgggcg ccttgttgcc
     4441 gggggtgacg ttgtcgaggg acatgcgctg tctttcttgt tgatgtgggt taagaggagt
     4501 caggattaac cctgatttta ctttcgcgcc cctttcgggc cgggatttgt cacgttttcg
     4561 ggctaaattc gcacggttgg ccaatcgacg ccccgagcga gggagcgagg aagcaacgca
     4621 gcgagggcgc cggctgtgtt gtcccattca ggccaaccag ccagcccctc acacgcgcaa
     4681 ccgcttgagt gaggagcaat caatgacagg caattcggcg ctgattctgg cgctcgtctg
     4741 cgggctgatc gcc
//
LOCUS       DAOMVO010000192         4692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5170_length_4692_cov_13.444468, whole genome shotgun sequence.
ACCESSION   DAOMVO010000192 DAOMVO010000000
VERSION     DAOMVO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_4692_cov_13.444468"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..2157
                     /locus_tag="PKV30_09495"
     CDS             169..2157
                     /locus_tag="PKV30_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRL30481.1"
                     /translation="MPLPHQLELLSPARDADIGIEAVNHGADAVYIGGPGFGARASAG
                     NALADIERLCRHAHRFDARIFVTLNTILRDDELEPARQLAWQLYEAGADALIIQDMGL
                     LQLDLPPIQLHASTQTDIRTPAKARFLQDAGLSQIVLARELDLQQIAAIRDALDPARC
                     AIEFFIHGALCVAYSGQCYISHAHTGRSANRGDCSQACRLPYQVLDAQGRFVAHDSHV
                     LSMKDNNQSLNLAPLIDAGVRSFKIEGRYKDMGYVKNITAHYRRLFDEILDARPELAR
                     ASSGRCSFTFAPDPNQNFNREFTDYFINGRQGDIGAFDTPKNPGQPIGFVTRVAADHF
                     ELMADDAALALHNGDGLCYWDAQKELVGAQTNRVEPLSGGAWRVWPKDPMAQLHDLRK
                     GTVINRNRSMDWVRTLEKKSADRRIGVWLKLSETADGLQLAATDEDGNTAAAQVQLAQ
                     AAPKDIATNDLKLRESLAKLGETVFTPIDIALDLQRPWFVPASVANALRRGAIAALEA
                     ARAAAWQRLPRAVPIDPPAPYPEDSITYLANVFNHAARDFYARHGVKVIDAAYESHEE
                     EGEVSLMITKHCVRFSLSLCPKQAKGVIGVKGTVKAEPLQLVNGKEKLTLRFDCKPCE
                     MHVVGKIKRGVMQAGAREAAEQALTFYRAQPLPAMFSD"
     gene            2227..2673
                     /locus_tag="PKV30_09500"
     CDS             2227..2673
                     /locus_tag="PKV30_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRL30482.1"
                     /translation="MKILLPVDGTELSLHETRFALQLLREGLKASFVLANVQEPASFY
                     EMVTADSPELIEGAALEAGEDLMEPSARLLREAGVPFETAVVQGDPAQSMLDLIEEHR
                     CDMVVMGSRALGPVRRWLEGGSTSQRLVQSSPVPVLLVTPPASDET"
     gene            2921..3827
                     /locus_tag="PKV30_09505"
                     /pseudo
     CDS             2921..3827
                     /locus_tag="PKV30_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            3858..>4692
                     /locus_tag="PKV30_09510"
     CDS             3858..>4692
                     /locus_tag="PKV30_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL30483.1"
                     /translation="MHAELVLAAGQPWHPAKVEAEQTAPSPHHLQLLRAAALCNDACP
                     SGDCDDKGHIVWQGDPTETALTAVAAQADLLKPELEEETPRVRELPFDSDRKRMTTFH
                     RDGAVFEAFTKGAPESVLPCCTAHWSPEGRRPLDQAAVLAEAERLAAQGLRVMAFASR
                     AWPQLPAEPDVEAIESELALIGLIGLIDPPREEAAAAVRDCISAGIRPVMITGDHPAT
                     ALAIAQRLGIVANDAANVITGAELTELDDAALKARVLDVRVYARVDPAQKIRIVQALQ
                     SH"
BASE COUNT          806 a         1674 c         1523 g          689 t
ORIGIN      
        1 cccctgtggg agcgggcttg cccgcgatgg cggcctcgga tgtggcgcag cgccccatcg
       61 cgggcaagcc cgctcccaca gaacaccggg ccagattgct attgattaaa tagctgtccg
      121 cgcttgccat acaagcgccc aagcccgatt tgaccgataa tcggcaccat gcccctgccc
      181 caccagcttg aactgctcag ccccgcgcgc gacgccgaca tcggcatcga ggccgtgaac
      241 cacggcgccg atgccgtcta catcggcggc cccggcttcg gcgcgcgcgc cagcgcgggc
      301 aacgcgctgg ccgacatcga gcgcctgtgc cgccacgcgc accgcttcga cgcgcgcatc
      361 ttcgtcacgc tcaacaccat cctgcgcgac gacgagctgg agcccgcgcg ccaactggcc
      421 tggcagctgt acgaagccgg cgccgacgcg ctgatcatcc aggacatggg gctgctgcaa
      481 ctcgacctgc cccccatcca gttgcacgcc agcacgcaga ccgacatccg cacaccggcc
      541 aaggcgcgtt tcttgcagga cgcgggcctg tcgcagatcg tgctggcgcg cgagctggac
      601 ctgcagcaga tcgcggccat ccgcgacgcg ctcgatccgg cgcgctgcgc catcgagttc
      661 ttcatccacg gcgcgctgtg cgtggcctac agcggccagt gctacatcag ccacgcccac
      721 accgggcgca gcgccaaccg gggcgattgc tctcaggctt gccgcctgcc ctatcaggtg
      781 ctcgacgcgc aaggccgctt cgtcgcgcac gactcgcacg tgctgagcat gaaagacaac
      841 aaccagagcc tgaacctggc gccgctcatc gacgccggcg tgcgcagctt caagatcgaa
      901 gggcgctaca aggacatggg ctacgtgaag aacatcaccg cccactaccg gcgcctgttc
      961 gacgagatac tggacgcgcg gcccgaactg gcgcgcgctt cgtcgggccg ctgcagcttc
     1021 actttcgcgc ctgatccgaa ccagaacttc aaccgcgaat tcaccgacta tttcatcaac
     1081 ggtcggcagg gcgacatcgg cgccttcgac acgcccaaga accccggcca gccgatcggc
     1141 tttgtcaccc gcgtcgccgc cgaccacttc gagctgatgg ccgacgacgc ggcgctcgcg
     1201 ctgcacaacg gcgacggcct gtgctactgg gatgcgcaaa aagagctggt cggcgcgcag
     1261 accaaccgcg tcgagccctt gagcggcggc gcctggcgcg tgtggcccaa ggacccgatg
     1321 gcgcaactgc atgacctgcg caagggcacg gtcatcaacc gcaaccgcag catggactgg
     1381 gtgcgcacgc tggagaaaaa gtccgccgac cgccgcatcg gcgtgtggct gaagctgtct
     1441 gaaaccgcag atggtttgca gctggccgcc accgatgaag atggaaatac ggccgcagcg
     1501 caagtccagc ttgcgcaagc agctcccaaa gacatagcaa ccaacgattt gaagttgcgc
     1561 gaatcactgg ccaagctggg cgaaacggtg ttcacgccga tcgacatcgc gcttgatctg
     1621 cagcgcccct ggttcgtgcc cgccagcgtg gccaacgcac tgcggcgcgg cgccatcgcc
     1681 gcgctcgaag ccgcccgcgc cgccgcctgg cagcgcctgc cgcgcgcggt gcccatcgac
     1741 ccgcccgcac cctaccccga ggattcgatc acctatctcg ccaacgtgtt caaccacgcc
     1801 gcgcgcgact tctacgcccg gcacggcgtg aaggtgatcg acgccgccta cgaaagccac
     1861 gaggaagaag gcgaagtcag cctgatgatc accaagcact gcgtgcgctt ttcgctcagc
     1921 ctgtgcccca agcaggccaa gggcgtgatc ggtgtgaagg gcaccgtcaa ggccgagccg
     1981 ctgcaactcg tcaacggcaa ggaaaagctc accctgcgct tcgactgcaa gccctgcgag
     2041 atgcacgtgg tcggcaagat caagcgcggc gtgatgcaag ccggtgcgcg cgaggcggcc
     2101 gagcaggcgc tgaccttcta ccgcgcgcag ccgttgccgg ccatgttttc tgattgaaat
     2161 cagcctccag tcgccgtata tactgcgcaa gcagctatct tttcaatagt atcgaggccg
     2221 acacccatga aaatcctgct ccccgtcgac ggcaccgaac tgtccctgca cgagacgcgc
     2281 ttcgccctgc agttgctgcg cgagggcctc aaagccagct tcgtgctggc caacgttcag
     2341 gagccggcca gcttctacga gatggtcacc gccgattcgc ccgaactgat cgagggcgcg
     2401 gcgctggagg ccggcgaaga cctgatggag ccttcagcgc gcttgctgcg cgaggccgga
     2461 gtacccttcg aaaccgccgt ggtgcagggc gacccggccc agtccatgct ggatctgatc
     2521 gaggaacacc gctgcgacat ggtggtgatg ggcagccgcg cgctcggacc cgtgcgccgc
     2581 tggctggagg gtggatcgac ctcgcaacgg ctggtgcaaa gctcgccggt gccggtgctg
     2641 ctggtcacgc cgccagccag cgacgagact tgattttttg gtcatcccgt tatccagtcc
     2701 tcgcccgatc agcgccggca gctattgatc gggtagcaaa acaccgcagc attccgctcg
     2761 ggttttcgcg gggcggtcat cggcacgcac ttcagtgtgc gcaacgatga cctcacccga
     2821 aactgccgga cccgacttgc ccttcccgcc ctcttccgac tggcacctgc gccccgccgc
     2881 cgaggtgctg caagcccagc aaaccgatgc cgccagaggc ctgagcgccg aagaagccgc
     2941 gcgccgcctg caagaacacg gcgccaacga actggcgacg cagcaagcgc gcccctggcc
     3001 gaagctgctg ctggaccagt tcaccgactt catgatcctg gtgctgctgg cggcggccgt
     3061 ggtgtcgggg ctgctgggcg aatgggtcga taccctcgtc atcgtcgtca tcgtggtgtt
     3121 gaacgcggtg atcggcttca cgcaggcgcg gcgggccgac caggcgattg cggcgctgcg
     3181 ccagctggcc gccgcgcagg cgcaggtact gcggggtggc cagccgcagc ccgtgcccgc
     3241 cgccgacctg gtgccgggcg acatcgtgct gctggaggcg ggcaaccaga ttccggccga
     3301 cctgcggctg ctggaggcgg cgcagctcaa ggtagataag tcgacgctga cgggcgaatc
     3361 ggtcaccgtc gagaaacgca ccgaagccct ggtcggcgac aagctggcgc tgggcgaccg
     3421 catcaacctg ggcttcaagg gcaccacggt gacccacggc cgcgcgcggc gtggtggtgg
     3481 ccaccggcat gggcaccgag ctgggcaagg tggccggcct gctgaacgac gccatggacc
     3541 gcagcacgcc gctgcagctg cggctggcgc gcttctcgaa gcagctgtcg ctgatcgtgc
     3601 tgctgatctg cgtcggcctg ttcgccttcg gcctgctgcg cggcgagccg tggctgctga
     3661 tgctgctcac cgccatcagc ctggccgtgg cggcgattcc cgaggcgttg ccggccgtcg
     3721 tcaccgtgct gctggccgtg ggcgccaagc gcatggcgga tttcaacgcg ctgatccgcc
     3781 gcctgccggc ggtggaaacg ctgggctccg tcagcgtgat ctgctccgac aagaccggca
     3841 cgttgacgca gaaccgcatg cacgccgagc tggtgttggc cgccggccag ccctggcacc
     3901 cggccaaggt cgaggccgag cagaccgcgc ccagcccgca ccacctgcag ctgctgcgcg
     3961 ccgccgcgct gtgcaacgac gcctgcccca gcggcgattg cgacgacaag ggccacatcg
     4021 tctggcaagg cgacccgacc gagaccgcgc tgacggccgt ggccgcgcag gccgacctgc
     4081 tgaagcccga gctggaagaa gaaacgcccc gcgtgcgcga gctgccgttc gactcggacc
     4141 gcaagcgcat gaccaccttt caccgcgacg gtgcggtgtt cgaggccttc accaagggcg
     4201 cccccgaaag cgtgctgccg tgctgcaccg cacactggtc gcccgaaggg cggcggccgc
     4261 tcgaccaggc cgccgtgctg gccgaggccg agcgcctggc cgcgcagggc ctgcgcgtga
     4321 tggccttcgc cagccgcgcc tggccgcagc tgccggccga gccggacgtg gaggccatcg
     4381 aaagcgaact ggcgctgatc ggcctgatcg gcctgatcga cccgccgcgc gaggaagccg
     4441 ccgccgcggt gcgcgactgc atcagcgccg gcatccgccc ggtgatgatc accggcgacc
     4501 acccggccac ggcgctggcc atcgcccagc ggctgggcat cgtggccaat gacgccgcca
     4561 atgtcatcac cggcgccgaa ctgaccgagc tggacgacgc ggcgctgaag gcccgcgtgc
     4621 tcgatgtgcg cgtctatgcc cgggtcgatc cggcgcagaa gatccgcatc gtgcaggcgc
     4681 tgcagtcgca cg
//
LOCUS       DAOMVO010000193         4688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5185_length_4688_cov_11.530758, whole genome shotgun sequence.
ACCESSION   DAOMVO010000193 DAOMVO010000000
VERSION     DAOMVO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5185_length_4688_cov_11.530758"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKV30_09515"
     CDS             <1..386
                     /locus_tag="PKV30_09515"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HRL30484.1"
                     /translation="APPAPAPVAPAPSPAPDDRASPADDSAAQTSQAIYAEGLRLYRA
                     GQYDDAIARFAAVQSPPSLRVQALKHTAFGHCVTNRLPLCQRAFQDALALSPAFRLTK
                     AEQGHPVWGPVFTKALQQSQPARRR"
     gene            complement(463..1644)
                     /locus_tag="PKV30_09520"
     CDS             complement(463..1644)
                     /locus_tag="PKV30_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30485.1"
                     /translation="MTAAPPAATPRLPAGIWVLGFVSLLMDISSEMIHALLPLFMVGT
                     LGMGVALVGLLEGLAEATALILKVCSGVISDWFGRRKPLAVLGYGLGAATKPLFALAT
                     GPGLIFTARLLDRVGKGIRGAPRDALVADIAPPEQRGAAFGLRQSLDTVGAFVGPLLA
                     VALMLLWANDFRAVFAVAVVPALLAVLLLMVGVREPARPAGAARSNPISLANLKRLPR
                     AYWWVVGVGAVFTLARFSEAFLVLRAEQLGVALAAVPLVMVAMNVVYSLTAYPFGKLS
                     DRVSHHGLLLAGLSVLVLADVVLAVSTHWVGLLLGVALWGVHMGMTQGLLAAMVAAQA
                     PADLRGTAFGFFNLVSGVALLAASVLAGLLWQYLGPAWTFGAGAAFSVLAMAALAWRA
                     D"
     gene            1739..2509
                     /locus_tag="PKV30_09525"
     CDS             1739..2509
                     /locus_tag="PKV30_09525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL30486.1"
                     /translation="MSQPDQSAPADPLRAPHQPISGYYQTEDERAGFLREIFDSTATD
                     YDKVERTLALGTGPWYRRRALERAGLAPGMRVVDVGFGTGLVAAQAIKIIGSADLLTG
                     VDPSPAMMGASPLAKQVKLVEGKAERIPLPDACADFLSMGYALRHVSDVSAAFSEFHR
                     VLKPGGRLCILEITKPEGAVGNALLKGYMRGWVPLAARLSGSATHTPRIWRYYWDSIE
                     ACAPPARILETLRAAGLVEANRYVELGIFSEYRARKPV"
     gene            2506..3300
                     /locus_tag="PKV30_09530"
     CDS             2506..3300
                     /locus_tag="PKV30_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL30487.1"
                     /translation="MTALARPPVIARRRVAWALGAAALPWALPGCAPIARPVTARMDS
                     LREPADPARRAETLLVLLPGAYDTPQDFVREGFVQAVRERGLPLDLLLPDAHIGYYNG
                     RLIVERLLHEVVRPARAAGYVRVWLAGISLGGYGSLLFARDHGALLDGVFVMAAFLGR
                     RDLPAAIAQAGGLAAWNGELPGADAHDLALWRWLSAYAGAPSPSHPPLWMGYGEDDRF
                     VMSNRLVAAVLPPAQVFNTEGGHQWAPWRRLWARFLDRAPWAGAPR"
     gene            3374..3903
                     /locus_tag="PKV30_09535"
                     /pseudo
     CDS             3374..3903
                     /locus_tag="PKV30_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
     gene            4010..>4688
                     /locus_tag="PKV30_09540"
     CDS             4010..>4688
                     /locus_tag="PKV30_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL30488.1"
                     /translation="MQQNFIANQSVASHTGRTLPVIDPSDGQPFDDIQRSDERDINDA
                     VRAARQCFEGPWSALSAAERGRLLMALSRKVAEHADELAAIEQRDCGKPTSQAAADAA
                     ALARYFEFYAGACDKFHGETIPYQNGYSVFTWREPHGVTGHVIPWNYPMQIFGRSVGG
                     ALAAGNCCVVKPAEDACLSLIRVAQLAAECGFPPGAINIVTGYGHEVGDALARHAGID
                     HISFTGSP"
BASE COUNT          704 a         1762 c         1566 g          656 t
ORIGIN      
        1 ccgcgccgcc cgccccggca cctgtggcgc ccgcgccctc gcctgccccc gacgaccgcg
       61 ccagccccgc tgacgacagc gccgcgcaaa ccagccaggc catctacgcc gagggcctgc
      121 gcctgtaccg tgccggccag tacgacgacg ccatcgcccg ttttgccgcc gtgcagtcac
      181 cgccgtcgct gcgcgtgcag gcgctcaagc acaccgcctt cggccattgc gtgaccaacc
      241 gcctgccgct gtgccagcgc gcgtttcagg acgcgctggc gctgtcgccc gcttttcggc
      301 tgaccaaggc cgagcagggg cacccggtgt gggggccggt gttcaccaag gctttgcagc
      361 agagtcagcc ggcgcggcgc cgctgacgcc atcgtccgcc gcgccgattg ctccacattc
      421 aatagctgct cgcgcttgcc agacaagcgc tggggctcgt tttcaatcag cgcgccaggc
      481 caacgccgcc atcgccagca cgctgaaagc cgcgccggcg ccgaacgtcc acgccggccc
      541 caggtattgc cacagcagcc ccgccagcac actggccgcc agcagggcga cgccactcac
      601 caggttgaaa aagccgaagg cggtgccgcg cagatcggcc ggcgcctggg ccgccaccat
      661 ggcagccagc aacccctgcg tcatgcccat gtgcacgccc cacagcgcca cgcccagcag
      721 caggcccacc cagtgcgtgc tgacggccag caccacgtcg gccagcacca gcaccgacag
      781 gccggccagc agcagcccgt ggtgcgacac gcgatccgac agcttgccga acggataggc
      841 ggtgagcgaa tacaccacgt tcatcgccac catcaccagc ggcaccgccg ccagcgccac
      901 gccgagttgc tcggcgcgca gcaccagaaa cgcctcggaa aagcgcgcca gcgtgaacac
      961 ggcgccgacg ccgaccaccc accagtaggc gcgcggcagg cgcttcaggt tggcgaggct
     1021 gatcgggttg ctgcgcgcgg cgccggccgg gcgcgccggc tcgcgcacgc ccaccatcag
     1081 cagcaacacg gccagcagcg ccggcaccac ggccaccgcg aacacggcgc gaaagtcgtt
     1141 cgcccacagc agcatcagcg ccaccgccag caaggggccg acaaaggcgc ccaccgtgtc
     1201 gagcgactgc cgcagcccga aggcggcgcc gcgctgctca ggcggcgcga tgtcggccac
     1261 cagcgcatcg cgcggcgcgc cgcgaatgcc cttgcccacg cggtccagca ggcgcgcggt
     1321 gaagatcagc cccggcccgg tggccagcgc aaacagcggc ttggtcgccg cgcccagccc
     1381 gtagcccagc accgccagcg gtttgcggcg gccaaaccag tcgctgatga cgcccgagca
     1441 caccttgagg atcagcgccg tcgcttcggc cagcccctcc agcaacccga ccagcgccac
     1501 gcccatgccc agcgtgccga ccatgaacag cggcagcagc gcatggatca tctcgctgga
     1561 gatgtccatc agcaagctga cgaaacccag cacccagatg ccggcgggca ggcgcggggt
     1621 ggcggcgggc ggtgcggcgg tcatggcggc tggcgacaaa ttttcggccg ccattggacc
     1681 atgggcccgt ccgcgccctt gcgccacaat ggcacgatcc tccgcgagcc cccaagtcat
     1741 gagtcaaccc gaccagtcgg cccccgccga cccgctgcgc gcgcctcacc agccgatcag
     1801 cggctattac cagaccgagg acgagcgcgc cggcttcctg cgcgagatct tcgattccac
     1861 cgccaccgat tacgacaagg tcgagcgcac gctggcgctc ggcaccggcc cctggtaccg
     1921 gcggcgcgcg ctggagcgcg ccggcctggc gcccggcatg cgcgtggtcg acgtcggctt
     1981 tggcaccggc ctggtggcgg cgcaggcgat caagatcatc ggcagcgccg atttgctgac
     2041 cggcgtcgac cccagccccg ccatgatggg cgccagcccg ctggccaagc aggtcaagct
     2101 ggtcgaaggc aaggccgagc gcattccgct gcccgacgcc tgcgccgact tcctcagcat
     2161 gggctacgcg ctgcgccacg tgagcgacgt cagcgcggcg ttcagcgaat tccaccgcgt
     2221 gctgaagccc ggcgggcgcc tgtgcatcct ggaaatcacc aagcccgaag gcgccgtggg
     2281 caatgcgctg ctcaagggct acatgcgcgg atgggtgccg ctggcggcgc ggctgtcggg
     2341 ctcggccacg cacacgccgc gcatctggcg ctattactgg gacagcatcg aagcctgcgc
     2401 gccgcccgcg cgcattctgg agacgctgcg cgcggccggt ctggtggagg ccaatcgcta
     2461 cgttgagctg ggcatcttct ccgaataccg tgcgcgcaag ccggtgtgac cgccctcgcg
     2521 cgcccgcccg tcatcgcgcg ccgccgcgtg gcgtgggcgc tgggcgccgc cgccctgccc
     2581 tgggccttgc ccggttgcgc acccatcgcc cgcccggtga ccgcgcgcat ggatagcctg
     2641 cgcgaacccg ccgacccggc gcgccgggcc gaaacgctgc tggtgctgct gcccggcgcc
     2701 tacgacacgc cgcaggactt cgtgcgcgaa ggcttcgtgc aggcggtgcg cgagcgcggc
     2761 ctgccgctcg atctgctgct gcccgatgcg cacattggct actacaacgg ccgcctgatc
     2821 gtcgaacgcc tgctccatga agtggtgcgg ccggcgcgcg cggccggcta cgtgcgcgtc
     2881 tggctggccg gcatttcgct cggcggatac ggctcgctgc tgtttgcgcg cgatcacggc
     2941 gcgctgctgg acggtgtttt cgtcatggcc gccttcctgg gccggcgcga cctgcccgcc
     3001 gccatcgcgc aggccggcgg cctggcagcg tggaacggcg agctgcccgg cgccgacgcg
     3061 cacgacctgg cgctgtggcg ctggctgagc gcttacgccg gcgcgcccag cccgtcgcac
     3121 ccgccgctgt ggatgggtta cggcgaggac gaccgctttg tgatgagcaa ccgcctggtg
     3181 gcggccgtgc tgccgcccgc gcaagtcttc aacaccgagg gcggccacca gtgggcgccg
     3241 tggcgccggc tgtgggcgcg ctttctggac cgcgcaccgt gggcgggcgc accgcgctga
     3301 tccgggcatc accgcattgc cggcgccgcc ggcaggccgt acagtggccg ttgacgctct
     3361 accggagacc gccatgaaaa ccctggtcga ccaactggcc cagtacgccg actaccaccg
     3421 cgaccggcgc aacatcgcca cgcacttcgt cggcatccca atgatcgtgc tggcggtgca
     3481 ggtgctgctg tcgcgccctg ccctgcaggc cggcgccttt gtgctgacgc ccgcgctgct
     3541 gaccacgctg ctggcctgcc tctgtactac gttgcgctcg accggccgct cggcgtgctg
     3601 atggccgcgc tgctggccgc cggcctggcg ctggccgggc agattgccgg gctgtccacc
     3661 agcgcctggc tgggctgggg cattggcctg ttcgtggtcg gctgggtgat ccagttcgtt
     3721 ggccacatct gggaagggcg caagccggcc tttgtcgacg atgtgatcgg cctggtgatc
     3781 gggcccttgt tcgtggtggc tgaactggtg tttgcgctgg gcctgcggcg cgacctgcac
     3841 gcggcgatca ccgggcgcgc cgggccgatg cgcggcggcg cgcgtgcgca aggcgcgcac
     3901 tgaagtttca ctattttttc aatagctgcc tgcgcttgtc tgacgtgcgc tggcggccga
     3961 tttggcttgc aaaaacgggc ctgccgatga tttgccgcac aatgaccgga tgcagcagaa
     4021 cttcatcgcc aaccagtccg tcgcctcgca taccggccgc acgctgccgg tgattgatcc
     4081 gtccgacggc cagccttttg acgacatcca gcgcagcgac gagcgcgaca tcaacgacgc
     4141 cgtgcgcgcc gcgcgccagt gcttcgaagg cccgtggagc gccttgagcg ccgccgagcg
     4201 cgggcgtctg ctgatggcgc tgtcgcgcaa ggtcgccgaa cacgccgacg agctggccgc
     4261 catcgaacag cgcgactgcg gcaagcccac aagccaagcc gccgccgacg ccgccgcgct
     4321 ggcacgctac ttcgagttct acgctggcgc ctgcgacaag tttcacggcg aaaccatccc
     4381 ctaccaaaac ggctacagcg tcttcacctg gcgcgagccg cacggtgtga ccggccacgt
     4441 gattccgtgg aactacccga tgcaaatctt cggccgcagc gtgggcggcg cgctggcggc
     4501 gggcaactgc tgcgtggtca agccggccga agatgcctgc ctgtcgctga tccgcgtggc
     4561 gcaactggcg gccgaatgcg gctttccgcc gggcgccatc aacatcgtca ccggctacgg
     4621 ccacgaggtg ggcgacgcgc tggcgcgcca tgcgggcatc gaccacatca gcttcaccgg
     4681 cagcccca
//
LOCUS       DAOMVO010000194         4678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5205_length_4678_cov_16.551157, whole genome shotgun sequence.
ACCESSION   DAOMVO010000194 DAOMVO010000000
VERSION     DAOMVO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_4678_cov_16.551157"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKV30_09545"
     CDS             complement(<1..292)
                     /locus_tag="PKV30_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HRL30489.1"
                     /translation="MRKSKLSPLTAVLSTALLLAATVATAQTPAPAASAGGHQGDVTP
                     AEQKYQGAPSPLANEPMHQTSNPKAPPMTQAEFDKARQIYFERCAGCHGVLRK"
     gene            complement(559..729)
                     /locus_tag="PKV30_09550"
     CDS             complement(559..729)
                     /locus_tag="PKV30_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30490.1"
                     /translation="MDTARLTLRQLTVALGVLLISGSAFVSIVQTPHTQPVTSAEVDG
                     ALMHLSEVVVAD"
     gene            complement(864..1181)
                     /gene="sugE"
                     /locus_tag="PKV30_09555"
     CDS             complement(864..1181)
                     /gene="sugE"
                     /locus_tag="PKV30_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HRL30491.1"
                     /translation="MSWIILVIAGLFEIGWAIGLKYTQGFTRLWPTLGTVGAMVISVG
                     LLGVAMRELPVGTAYAVWTGIGAVGTVILGIVLFGDPANAPRLVCVGLIVAGILGLKL
                     TSA"
     gene            complement(1388..2605)
                     /locus_tag="PKV30_09560"
     CDS             complement(1388..2605)
                     /locus_tag="PKV30_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRL30492.1"
                     /translation="MNPDAHQLWKGPRWALAVLLAVLGMLGPFSIDTYIPAFSAIAKS
                     LQATPVQMQQTLSAYLFGFAFMNLFHGALSDSFGRRPVILWGLALFTLASLGCALSQS
                     VGQLIFFRAVQGLSCGAGVVVSRAVVRDMFPPADAQKVMSQITIFFGVAPAVAPIIGG
                     VIAEYLDWHSIFWFLVGVGVVLWVANWRLLPETLHRDDRQPFQVGNLMSGYWQLLSDP
                     RFMLLALASGVPFNGLFLYVLSAPAFLGDILQLRPTQFFWFFVLSISGIMAGSWLSGR
                     KAGKVLPKNQIMQGFRIMLAVSVLNIAANALFAPHALWSLWPVTVFAFGWALMVPVVT
                     LLVLDLHPERRGMASSLQAVVGSTANGIVAGAVAPLVMHSAMGLAVMSAAFMAVGLVA
                     WLWLRQRWPAIGE"
     gene            complement(2736..>4678)
                     /gene="rpoD"
                     /locus_tag="PKV30_09565"
     CDS             complement(2736..>4678)
                     /gene="rpoD"
                     /locus_tag="PKV30_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRL30493.1"
                     /translation="RMKISKAKERALMKEFGLDDSVLSEEEMNLRRANLKALLKLGKT
                     RGYLTHVEISDHLPDKLFDAETLEAVIATLNDLGVAVYEQTPDAETLIITDNAPTGSS
                     EEEAEEAAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMAM
                     MEAISASPATIAEILRMAAEIREGKVVINTIVDGFSDANENDDYVAEEDFDEYDEEDD
                     DDGKGGSKAMTRLLEKLKTEALERFDEIASLFEKVHKAYDKEGWGSPNYVKSQHALSQ
                     RLMSIRFTAKTIEKLCDLLRSQVDDVRKYERELRRIIVDKCGYPQELFIADFSGRDKA
                     GHKVASNVLNLKWVEKQAAAGKPWSAVMERNIPPIQDLQQKLIDLQSQVVVPLDELKS
                     INKRMNEGEAASRDAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDK
                     FEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQEFGF
                     EPDAGILAEKMEIPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDGNNTAPIEA
                     AMQAGLRDVVKDILDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEA
                     KALRKLKHPSRSDKLRSFIDSL"
BASE COUNT          793 a         1502 c         1504 g          879 t
ORIGIN      
        1 ccttgcgcag cacgccgtgg cagccggcgc agcgctcgaa gtagatctgg cgcgccttgt
       61 cgaattcggc ctgcgtcatc ggcggggcct tggggttgct cgtctggtgc atcggctcgt
      121 tggccaatgg cgacggcgcg ccctggtact tttgctcggc gggggttacg tcgccttggt
      181 gaccgccagc ggatgcggca ggagcggggg tttgcgccgt cgcgacggtc gccgccagca
      241 gcagcgccgt gctcaacacc gcggtgagtg gagagagctt ggacttcctc atagatgttc
      301 tcatcgtatt tttccgtgtc accggaacgg ccttgttggt gggttgtccg gcgatggccg
      361 tgaaatcgat cggaacaacc tgattcttgg aggggtcaag cctagcgtcc ttgacttggt
      421 tcaagtcaag ttttcatgcg tccgcaggca acgacaacag ccgtcgaata tgacggcgat
      481 caagaaaacg gctgattttg ggcggccggg ttggccgcgt gggctaaccg gccttcaggc
      541 gcgcgcgtgg gccgaatttc agtcggcgac gaccacttcg ctcaggtgca tcaaggcgcc
      601 atcgacttcg gccgaggtca ccggctgcgt gtgcggtgtc tggacgatgg acacgaaggc
      661 gctgcccgaa atcagcagca cgcccaaagc gacggtcagt tgacggagcg tgaggcgggc
      721 ggtgtccacg gtgtgcgtcc tggattgggc gatcgggttg ctgggggtga cgtgatggtc
      781 gcccggcggc cgcgcgcgcc gtgtcagaca agaaccaaac gcacgtcagc cgccatcgca
      841 atgtctcgag cgcccggcct tcatcaggcg ctggtcagct tcaggcccag gatgccggcc
      901 acgatcaggc ccacgcagac caggcgcggc gcattggccg ggtcgccgaa cagcacgatg
      961 ccgaggatga cggtgccgac ggcaccaatg cccgtccaca ccgcgtaggc cgtgccgacg
     1021 ggcaattcgc gcatggccac gccaagcagg ccgacgctga tcaccatcgc gcccacggtg
     1081 cccagcgtgg gccacaggcg cgtgaaacct tgggtgtact tgaggccgat ggcccagccg
     1141 atctcgaaca ggccggcgat gacgaggatg atccaggaca tggtgacgac ttccttgaac
     1201 aaaggcgctg gggtcgtccc cacggagggt gatgcacggc gggtcgtccc gccgcgtttg
     1261 ctattgtaaa aatagctgct tgcgcttggt ggacaagcgc tggaggccga ttttatgcaa
     1321 agtggtcggc gcccggcggt gtggcgtggc aaccacccac ggcgcgccgg gttgccagcc
     1381 ggccgaccta ttccccgatc gccggccagc gctggcgcag ccacagccag gccaccagcc
     1441 cgaccgccat gaaggccgcc gacatgacgg ccaggcccat cgccgaatgc atgaccagcg
     1501 gcgccaccgc gcccgccacg atgccgttgg ccgtcgagcc gaccaccgcc tgcagcgacg
     1561 acgccatgcc gcgccgctcg gggtgcagat cgagcaccag cagcgtcacc accggcacca
     1621 tcagcgccca gccgaaggcg aacaccgtca ccggccacag cgaccacagc gcgtgcggcg
     1681 cgaacagcgc gttggcggcg atgttgagca ccgacaccgc cagcatgatg cgaaagccct
     1741 gcatgatctg gttcttcggc agcaccttgc ccgccttgcg cccggacagc cacgagccgg
     1801 ccatgatgcc gctgatcgac agcacgaaga accagaagaa ctgcgtcggc cgcaactgca
     1861 ggatgtcgcc caggaaagcc ggcgccgaca gcacgtacag aaacagcccg ttgaacggca
     1921 cgcccgaggc cagcgccagc agcatgaagc gcggatcgct cagcaactgc cagtagccgc
     1981 tcatcaggtt gccgacctgg aacggctggc ggtcatcgcg gtgcagcgtc tccggcaaca
     2041 gccgccagtt ggccacccac agcaccacgc cgacgccgac cagaaaccag aagatcgaat
     2101 gccagtccag gtattcggcg atcacgccgc cgatgatggg cgccacggcg ggcgctacgc
     2161 caaagaagat ggtgatctgg ctcatcacct tctgcgcgtc ggccggcggg aacatgtcgc
     2221 gcaccacggc gcgcgagacc accacgccgg cgccgcacga caggccctgc accgcgcgga
     2281 agaagatcaa ctgccccacg ctttgcgaca gagcgcagcc aagcgatgcc agcgtgaaca
     2341 gcgccaggcc ccacaagatg accggccggc gcccgaagct gtccgacagc gcgccatgaa
     2401 acaggttcat gaaggcaaag ccgaacaggt aggcgctcag cgtctgctgc atctgcaccg
     2461 gcgtcgcctg cagcgacttg gcaatcgccg aaaacgccgg gatgtaggtg tcgatcgaga
     2521 acggcccgag catgcccagc accgccagca gcacggccag tgcccagcgc gggcctttcc
     2581 acagttgatg ggcgtcgggg ttcatgcttg aaaacgaata ccggacgcga aggacgcgaa
     2641 aactcgccaa ggacgcgaaa gaaaacccac tcaagagttt tctttcgcgt ccttcgcgaa
     2701 tcctttgcgt ccttcgcgtc cggctgttgg gcttcttaca gcgaatcgat gaagctgcgc
     2761 agcttgtccg agcggctcgg gtgcttcagc ttgcgcagcg ccttggcttc gatctggcgg
     2821 atgcgctcgc gcgtgacgtc gaactgcttg cccacttctt ccagcgtgtg gtcggtgctc
     2881 atctcgatgc cgaagcgcat gcgcagcacc ttggcttcgc gcggggtcag gctgtcgagg
     2941 atgtccttga ccacgtcgcg caggccggcc tgcatggccg cttcgatcgg cgcggtgttg
     3001 ttgccgtcct cgatgaagtc gcccaggtgg ctgtcgtcgt cgtcgccaat cggcgtttcc
     3061 atggagatcg gctccttggc gatcttcatg atcttgcgga tcttgtcctc gggaatctcc
     3121 atcttctcgg ccaggatgcc ggcgtcgggc tcgaaaccga actcctgcag gtgctggcgc
     3181 gagatgcggt tcatcttgtt gatggtctcg atcatgtgca ccgggatgcg gatggtgcgc
     3241 gcctggtcgg cgatcgagcg cgtgatggcc tggcgaatcc accaggtggc gtaggtcgag
     3301 aacttgtagc cgcggcggta ttcgaacttg tccaccgcct tcatcaggcc gatgttgccc
     3361 tcttgaatca aatcgaggaa ctgcaggccg cggttggtgt acttcttggc aatcgagatc
     3421 accaggcgca ggttggcctc gatcatctct ttcttggcgt cgcgcgaggc ggcttcgcct
     3481 tcgttcatgc gcttgttgat gcttttcagc tcatcgagcg gcaccaccac ctgcgactgc
     3541 aggtcgatca gcttttgctg cagatcctgg atcggcggaa tgttgcgctc catcaccgcg
     3601 ctccacggct tgccggcggc ggcttgtttt tcgacccatt tcaggttgag cacgttcgag
     3661 gcgaccttgt ggccggcctt gtcgcgcccc gagaagtcgg cgatgaacag ctcctgcggg
     3721 tagccgcact tgtcgacgat gatgcggcgc agttcgcgct cgtacttgcg cacgtcgtcc
     3781 acctggctgc gcagcaggtc gcacagcttc tcgatggtct tggcggtgaa gcggatgctc
     3841 atcagccgct gggacagcgc gtgctgcgac ttcacgtagt tgggcgagcc ccagccttcc
     3901 ttgtcgtagg ccttgtgcac cttctcgaac agcgaggcga tctcgtcaaa gcgctccagt
     3961 gcctcggtct tgagcttttc cagcaggcgc gtcatggcct tggagccgcc cttgccgtcg
     4021 tcgtcgtctt cttcgtcgta ttcgtcgaag tcttcctcgg ccacgtagtc gtcgttttcg
     4081 ttggcgtcgg agaagccatc gacgatggtg ttgatgacca ccttgccttc gcggatctcg
     4141 gcggccatgc gcaggatttc ggcaatcgtc gccgggctgg cgctgatggc ttccatcatg
     4201 gccatcaggc cgccttcgat gcgcttggcg atctcgatct cgccctcgcg cgtgagcagc
     4261 tccaccgtgc ccatctcgcg catgtacatg cgcaccgggt cggtggtgcg gccgaattcg
     4321 ctgtccacgg tcgacagcgc ggcctcggcg gcttcttcag cctcttcctc gctggagccg
     4381 gtgggcgcgt tgtcggtgat gatcagcgtc tcggcatcgg gcgtctgctc gtacacggcc
     4441 acgcccaggt cgttcagcgt ggcgatcacg gcttcaagcg tctcggcgtc aaacagcttg
     4501 tcgggcagat ggtcggagat ttcgacgtgc gtcaggtagc cgcgcgtctt gcccaatttc
     4561 agcagcgcct tcaggttggc gcggcgcagg ttcatctctt cctcggacag cacgctgtcg
     4621 tcaaggccga attccttcat caaggcgcgt tccttggcct tgctgatctt catgcgca
//
LOCUS       DAOMVO010000195         4633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5278_length_4633_cov_13.309305, whole genome shotgun sequence.
ACCESSION   DAOMVO010000195 DAOMVO010000000
VERSION     DAOMVO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5278_length_4633_cov_13.309305"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1479)
                     /locus_tag="PKV30_09570"
     CDS             complement(328..1479)
                     /locus_tag="PKV30_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL30494.1"
                     /translation="MSRWLKTALVLLTLIAVVALGWRGYAARQATRQAQSAPVKDPVI
                     ELSAIDMATAHVVELTSGLPISGALKAVNSAIVKARVPGELQGLTVREGDRVEAGQVI
                     ARVESTEYADRVRQAQQQADAAKAQVEIAQRQVDNNAALVRQGFISKTAADNSLASLN
                     TAQANHRAAQAGIDVLRKSLADTVLRAPISGQVSQRLAQPGERVAPEARIVEIVDLSR
                     LELEASISPADSVAVRVGQQALLRIAGVAQPVAATVARINPSAQAGSRSVLIYLTVAA
                     QPGLRQGLFAQGQLATETQRALAVPLNAVRTDKPLPYVQVVKGDRIAHRTVQTGVRGE
                     VDGELWVAVQGVADGVRVLRGAAGVVREGTQVKLAAPVAPASAAQGVRS"
     gene            complement(1476..2123)
                     /locus_tag="PKV30_09575"
     CDS             complement(1476..2123)
                     /locus_tag="PKV30_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL30495.1"
                     /translation="MSVIQDSPAPATKRARRKEARPGELLEAALALFVEKGYAATRVE
                     EVAARAGVSKGTLFLYFPSKEELFKAVVRENAGRHLQDAFREVEAYTGSSAELLHEFI
                     RRWWTQYGGTPAAGLTKLIMSEAANFPDLAQYYQDEVVQPSHELVRRIVQRGIARKEF
                     RAIDLTLASHLIVAPLVQMVTWRFSLAPCCPATQMPDPLKLLDMHADMAVRGLKI"
     gene            complement(2362..3900)
                     /locus_tag="PKV30_09580"
     CDS             complement(2362..3900)
                     /locus_tag="PKV30_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRL30496.1"
                     /translation="MPAAMDLLVLPLLAAALLVFFSVLAGLFSARAGFSFLLVFLLAG
                     ILAGEDGPGGIVFDDVKLSFWVGNVALAVILLDGGLRTDFATFRTGLKPALLLASVGV
                     VMSAALTAFGAWWLMGLDWRLAMLLGSIVGSTDAAAVFALLRSSGVRLNERVAATLEI
                     ESGMNDPMAVYLTIAFIGIALAGTGGTAADGGALGPWGEAIKALVQQFGLGGVIGGVA
                     GVALAALLKRSFPRFAAQQQGAASGGGILALLCVSLGLAVFAATSWVGGSGFLAVYVM
                     GVTARARAREAVAPALSALDGYAWLSQAAMFLLLGLLLTPHQLLPGLAPALGVAAFLL
                     LVARPLTVWLCLLPFHFERREVLYISWVGLRGAVPIVLAVFPLMAGVPGARTLFNAAF
                     VVVLTSLILQGSTIALAARKLGLNLPEADDESARRLVFGDFALEGRTPVADVCGFYGL
                     PEPPNADWPLERWIGHALHRPPVVGDHVQLGSAELSVRAMEGARVLIVGLRLPARVDA
                     DDGA"
     gene            complement(4047..>4633)
                     /locus_tag="PKV30_09585"
     CDS             complement(4047..>4633)
                     /locus_tag="PKV30_09585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRL30497.1"
                     /translation="YGVEPRDWLLNHGALPLEVERAWANISQQAIDCIAASALSTSAG
                     SQNDSDSDGASRIATLRLHADCHPGNILWTPSDLPGGGPHFVDLDDCRSGPAVQDLWM
                     LLSGERHEQQAQLGALLDGYEQLREFDRRELALIEPLRTLRLVHYSAWVARRWDDPAF
                     PAAFPDFGTPDYWRGQVDTLVEQLEAMQAPPLVA"
BASE COUNT          774 a         1663 c         1510 g          686 t
ORIGIN      
        1 tgctgcgctg cgcgcccgcc ccacgccctg cgcttctcgg cacggccaga aggggttttg
       61 aattcctcac tcgggccatc gctacgctcg gccctggctc gatcagcgcc gctgcttcgc
      121 gcggttctat ttgctccctc tccctctggg agaggcttgg cctgtcctga gcttgtcgaa
      181 ggagggtgag ggcgagccag ttcaaaaatc gtagctgctc gcgcttgccc cacaagcgct
      241 ggaggtgtat ttgccttgaa tcgattggtc ggcgccgtag ggtgggtgct tgcacccacg
      301 cattcgcccg cgtgctcgcc aagccgctca cgaccgcacg ccctgcgccg cgctggccgg
      361 cgcaacgggt gcggccagct tgacctgcgt gccctcgcgc accacccccg ccgcgccacg
      421 cagcacacgc acgccatcgg ccacgccttg caccgccacc cacagttcgc catcgacttc
      481 gccgcgcacg ccggtctgca ccgtgcggtg ggcgatgcgg tcgcctttca ccacctgcac
      541 atagggcaag ggcttgtcgg tgcgcacggc gttcaacggc acggccagcg cgcgctgcgt
      601 ttcggtggcc agttgcccct gcgcaaacag cccctggcgc aggccaggct gcgcggccac
      661 cgtcaaataa atgagcacgc tgcggctgcc ggcttgcgca ctggggttga tgcgcgccac
      721 ggtggcggca acgggttgtg ccacgcctgc gatgcgcagc agcgcctgct ggcccacgcg
      781 cacggccacc gaatcggcgg ggctgatgga ggcttccagc tccagccggc tcaaatcgac
      841 gatttcgacg atgcgcgcct cgggcgccac gcgctcgccg ggctgcgcca ggcgctgcga
      901 tacttgtccg ctgatgggtg cgcgcagcac ggtgtcggcc agcgacttgc gcagcacgtc
      961 gataccggcc tgcgcggcgc ggtggttggc ctgcgcggtg ttcaggctgg ccagcgaatt
     1021 gtcggccgcg gttttggaga taaagccttg ccgcaccagc gcagcgttgt tgtcgacctg
     1081 gcgctgggcg atctctacct gcgccttcgc cgcatcggcc tgctgttgcg cctggcgcac
     1141 gcggtcggcg tattcggtgg attcgacgcg ggcgatgacc tggccggcct ctacccggtc
     1201 gccttcgcgc acggtcaggc cctgcaactc gcccggcacg cgcgccttga cgatggccga
     1261 gttgacggcc ttcagcgcgc cgctgatggg caagccgctg gtcagctcga ccacgtgcgc
     1321 ggtggccata tcgatggcac tcagctcgat caccgggtct ttcacaggtg ccgattgcgc
     1381 ctgcctggtc gcctgccgcg ccgcatagcc gcgccagccc agtgccacga ccgcgatcag
     1441 ggtcagcaga acgagcgcgg tcttcagcca gcggctcata tcttcagccc gcgcaccgcc
     1501 atgtcggcgt gcatgtcgag cagtttcagc ggatcgggca tctgcgtggc cgggcagcag
     1561 ggcgccagcg aaaaacgcca ggtgaccatc tgcaccagcg gcgcgacgat caggtgcgac
     1621 gccagcgtca aatcgatggc gcgaaattcc ttgcgggcga tgccgcgctg cacgatgcgg
     1681 cgcaccagtt catggctggg ctgcaccacc tcgtcctggt agtactgcgc caggtcgggg
     1741 aagttggccg cctcgctcat gatcagcttg gtcagcccgg cggcgggcgt gccgccgtac
     1801 tgcgtccacc agcggcggat gaattcgtgc agcagctcgg cgctgctgcc ggtgtaggcc
     1861 tcgacttcgc ggaaggcgtc ctgcaggtgg cggccggcgt tttcgcgcac cacggccttg
     1921 aacagctctt ccttgctggg aaagtacaga aacagcgtgc ccttggagac gccggcacgc
     1981 gcggccactt cttcgaccct cgtggcggca tagccctttt cgacgaacag cgccaaggcg
     2041 gcttcaagca actcgcccgg gcgtgcctct ttgcggcggg cgcgcttggt cgcgggggcg
     2101 ggggaatctt gaatgaccga catcaaatta ctgactgact ggttagtaat attatgcagt
     2161 cggccggggc gagtcaagct tcggcgcgca gtgtcgtagg gtgggtgctt gcacccacgt
     2221 ttcgatcccg agcgttcgcc gtccgcgtag gtgcaagcac ccaccctaca aaaatcatag
     2281 ctgcccgcgc ttgcaaataa agcgccagcg gccattttca tggtcagtcg atgagcgcct
     2341 ggcgcccagg ccatccccgc ctcacgcccc atcgtccgca tcaacccgcg ccggcagccg
     2401 cagccccacg atcagcacgc gcgcgccctc catggcgcgc acgctcagct cggcggagcc
     2461 cagttgcacg tggtcgccca ccaccggcgg gcggtgcagg gcgtggccga tccagcgctc
     2521 cagcggccaa tcggcgttgg gcggctcggg caggccgtag aaaccgcaca cgtcggccac
     2581 cggcgtgcgg ccttccagcg caaagtcgcc aaacaccagg cgccgcgccg attcgtcgtc
     2641 ggcctcaggc aaattcaggc ccagcttgcg cgccgccagc gcaatcgtcg agccctgcag
     2701 gatgagcgag gtcagcacca ccacaaaagc cgcgttgaac agcgtgcgcg cgccgggcac
     2761 gccagccatc agcggaaaca ccgccagcac gatgggcacc gcgccgcgca acccgaccca
     2821 cgagatgtag agcacctcgc gccgctcgaa gtgaaacggc agcaggcaca gccacactgt
     2881 gagcggccgc gccaccagca gcaagaacgc cgccacgccc agcgccggcg ccagccccgg
     2941 cagcagctgg tgcggcgtca gcagcagccc cagcagcaag aacatcgccg cctggctcag
     3001 ccaggcgtag ccgtcgagcg ccgacagcgc cggcgccacc gcttcgcgcg cgcgggcgcg
     3061 cgccgtcacg cccatcacgt acaccgccag aaagcccgag ccgccgaccc agctggtggc
     3121 cgcaaacacc gccaggccca gcgacacgca cagcagcgcc agaatgccgc cgccgctggc
     3181 cgcgccctgc tgttgcgcgg caaagcgcgg aaagctgcgc ttgagcagcg ccgccagtgc
     3241 cacgcccgcc acgccgccga tgacgccgcc caggccaaac tgctgcacca gcgccttgat
     3301 cgcctcgccc cacggcccca acgcgccgcc gtctgccgcc gtgccgccgg tgccggccag
     3361 cgcgatgccg atgaaggcaa tggtcaggta gaccgccatc ggatcgttca tgcccgattc
     3421 gatttccaac gtggcggcca cgcgctcgtt cagccgcacg cccgacgagc gcagcagcgc
     3481 aaacacggcc gccgcgtcgg tcgagccgac gatggaaccc agcagcatgg ccagccgcca
     3541 gtccagcccc atcagccacc aggcgccgaa cgcggtgagc gccgcgctca tcaccacgcc
     3601 cacgctggcc agcagcagcg cgggcttcag cccggtgcga aaggtggcga aatcggtgcg
     3661 caggccgcca tcgagcagga tcaccgccag cgccacgttg ccgacccaaa aactcagctt
     3721 gacgtcgtca aagacgatac cgcccggccc gtcttcgccc gccagaatgc cggccagcag
     3781 aaacaccagc agaaacgaga acccggcgcg cgccgagaac agccccgcca gcacgctgaa
     3841 aaacaccagc agcgcggcgg caagcagcgg caacacgagc aggtccatgg cggcgggcat
     3901 ggacgaaaat tagcagatgg cgtgccggtg gcgcgaaaaa aacgggcgcc aacggtgatt
     3961 gctacgaagt agatagctgc ctgcgcttgt gggacgtgcg ctggcggcct gtttgacttg
     4021 aaattcttga cgacgcgatg ccaccgtcac gccaccagcg gcggcgcctg catggcttca
     4081 agctgctcga ccagcgtgtc gacctgcccg cgccagtagt ccggcgtgcc gaagtcggga
     4141 aaggcggcgg ggaaggccgg gtcgtcccag cgccgcgcca cccaggcgct gtagtggacg
     4201 aggcgcagcg tgcgcagcgg ctcgatcagc gccagctcgc ggcggtcgaa ttcgcgcaac
     4261 tgctcgtagc cgtcgagcag cgcacccagt tgcgcttgct gctcgtgccg ctcgcccgag
     4321 agcagcatcc acaaatcctg caccgccggg ccgctgcggc agtcgtccag gtcgacaaaa
     4381 tgcgggccgc cgccgggcag gtcggacggt gtccacagga tgttgcccgg gtggcagtcg
     4441 gcgtgcaggc gcagcgtggc gatgcggctc gcgccgtctg aatctgaatc attttggctt
     4501 ccagcgcttg tagataaagc gctggcagct atgcaatcga tagcttgctg gctgatgttg
     4561 gcccacgcgc gctcgacctc cagcggcagc gcgccgtggt tcagcagcca gtcgcgcggc
     4621 tcgacgccgt agc
//
LOCUS       DAOMVO010000196         4571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5385_length_4571_cov_14.554252, whole genome shotgun sequence.
ACCESSION   DAOMVO010000196 DAOMVO010000000
VERSION     DAOMVO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5385_length_4571_cov_14.554252"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..978
                     /locus_tag="PKV30_09590"
     CDS             94..978
                     /locus_tag="PKV30_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRL30498.1"
                     /translation="MAEPHPARSGERPAEREDRRSLFQKLVEFIRPGPDSTDELIGTL
                     AEAEDNEVIDAESRVMLEGVLRMADMTAGDAMVAAPRMDLLDIDAPYEALLLEVIRTA
                     HSRFPVYQGERENIIGILLAKDLLKLQRAPELNVRTLLRPAVFVPESKGLNDLLRAFR
                     ANRNHLAMVVDEFGRTAGLITIEDVLEQIVGEIEDEFDIDADEGDIFALADNTWRVAG
                     DTPLERVNESFGVHLGADPDGDLEVDFDTIGGLIAHEMGHVPKRGERFEMGGLDFHVL
                     HTKGGAVRWFKVARVDPP"
     gene            1120..2742
                     /gene="lnt"
                     /locus_tag="PKV30_09595"
     CDS             1120..2742
                     /gene="lnt"
                     /locus_tag="PKV30_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRL30499.1"
                     /translation="MRLLHVLASPPMALVAGVVHALSIGDPWTGQPHWWLQWLSLAAL
                     VWQLAHRAERQVSWSASTWARPPTGLPWRRAVLLGWLFALGWLAGTFWWLFISMHTYG
                     GLNPVLAVAAVLGLAGFLALYYAAAAGAFVALASARVLQRQPVLSALLFAALWTLAEL
                     ARGQWLTGFPWGAGGYAHVDGPLAFLARYVGVYGIGFVAAALAALLALSGHVRWRSGR
                     TLVGAALLLALGAGLWGWRFCDVNLCYTPAAGHERLVTEVALMQGNIPQDQKFVPGTG
                     VADSLRWYGEQLRDVRTPLVVAPETALPLLPRQLPEGYWQALLDRFSGPNQAALLGVP
                     LGDMEAGYTNSVVGMKAGQAAPYRYDKHHLVPFGEFIPPFFRWFTEMMNIPLGDFTRG
                     SVGQASFEWQGQRLAPNICYEDLFGEELAARFANPAAAPTAFVNVSNIGWFGDTVAID
                     QHLAISRMRALEFERPMLRATNTGATAIIDHRATVTHLLPRLTRGVLVGHFEGRTNIT
                     PFARWAAVAGLWPLWIGCALLVLACLALRRQS"
     gene            2789..3313
                     /gene="fabA"
                     /locus_tag="PKV30_09600"
     CDS             2789..3313
                     /gene="fabA"
                     /locus_tag="PKV30_09600"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HRL30500.1"
                     /translation="MAESFSYDQLIASGEGRLFGPDAGRLPLPPMLMFDRITHIDADG
                     GAHGLGRIEAELDVNPDLWFFACHFQGDPVMPGCLGLDAMWQLIGFYLTWLRLPGRGR
                     ALGAGEVKFTGEVGPDVKRVTYQIDIKRVIKRKLNMAIGDARLLADGKEIYVANDLRV
                     GLFLRPGEGQESSA"
     gene            3310..4530
                     /gene="fabB"
                     /locus_tag="PKV30_09605"
     CDS             3310..4530
                     /gene="fabB"
                     /locus_tag="PKV30_09605"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRL30501.1"
                     /translation="MSKQRVVITGAGIVSCIGNDLASVEASLREGRSGIRAVPEFAQL
                     GLRSQVGGKPEIDIEARIDRKQLRFMGDAAAYAQIALEDAIKQAGLTPNQVSHPRTGL
                     IMGSGGGSPANQIEAADTLREKGIRRVGPYQVTRCMSSTVSACLATNFKVKGINYSIT
                     SACSTSAHCIGAAAQQIAWGHQDVMFAGGGEELSWGMALLFDGMGAMSAKYNETPEKA
                     SRAYDASRDGFVIAGGGGAVVLESLAHARARGATILAEVVGFGATSDGADMVAPSGDG
                     AIACMQQALAGLDGGVDYINTHGTSTPVGDMQEVRAMRAVFGDAVPPFSSTKSLTGHS
                     LGATGVQEAIYCLIMLQKSFIAGSINVDTPDPSLGDLPLVTQTRDADVRTALSNSFGF
                     GGTNASLVLRRFDS"
BASE COUNT          675 a         1555 c         1590 g          751 t
ORIGIN      
        1 gcgcgcccac gcggcgcggc gtccgacgtg cgggcgcggg ggcttgggct atccttgcga
       61 tcccgacacc accgcacgtc ctgcaccccc accgtggctg agccgcaccc tgcccgctcc
      121 ggcgagcgac ccgccgagcg cgaagacagg cgcagcctgt tccagaaact ggtcgaattc
      181 atccggcccg ggcccgactc caccgacgag ctgatcggca ccctggccga agccgaggac
      241 aacgaggtca tcgacgccga gagccgcgtc atgctcgaag gcgtgctgcg catggccgac
      301 atgaccgccg gcgacgccat ggtggcggcg ccgcgcatgg atctgctgga catcgacgcg
      361 ccgtacgaag cgctgctgct ggaagtcatc cgtaccgcgc actcgcgctt tccggtctac
      421 cagggcgagc gcgagaacat catcggcatt ctgctggcca aggatttgct caagctgcag
      481 cgcgcgcccg agctgaatgt gcgcacgctg ctgcgcccgg cggtgttcgt gcccgaaagc
      541 aaggggctga acgatttgct gcgcgcgttt cgcgccaacc gcaaccacct ggcgatggtg
      601 gtggacgaat tcggccgcac cgccggcctg atcaccatcg aggacgtgct ggagcagatc
      661 gtcggcgaga tcgaggacga gttcgacatc gacgccgacg agggcgacat cttcgccctg
      721 gccgacaaca cctggcgcgt ggccggcgac accccgctcg agcgcgtcaa cgaatccttc
      781 ggcgtgcacc tgggcgccga cccggacggt gatctggagg tcgacttcga caccattggc
      841 ggcctgatcg cgcacgaaat gggccacgtg cccaagcgcg gcgagcgctt tgagatgggc
      901 ggccttgatt ttcatgtgct gcataccaag ggcggtgcgg tgcggtggtt caaggtagcg
      961 cgcgttgatc cgccgtgatt tctcccccct gagtcggcct gcggccgcct tcccccaggg
     1021 gacaacgctg gcggccgggg ggaccccggc cgcggcgttc cggaatggcc tgctccgcgc
     1081 ccttctgatt gaccggctca cgtagattaa cgttcgcaca tgcgactgct tcacgtgctg
     1141 gcttctccgc ccatggcgct ggtggcgggc gttgtgcatg cgctgtcgat cggcgacccg
     1201 tggacgggcc agccgcactg gtggctgcaa tggctcagcc tggcagcgct ggtgtggcag
     1261 ttggcgcacc gggccgagcg ccaggtgtcg tggagcgcca gcacctgggc ccgcccgccc
     1321 accggcctgc cgtggcggcg cgcggtgctg ctgggctggc tgttcgcgct cggctggctg
     1381 gcgggcacct tctggtggct gttcatctcg atgcacacct acggcggcct gaacccggtg
     1441 ctggcggtgg cggcggtgct ggggctggcg ggttttctgg cgctgtatta cgcggcggcg
     1501 gcgggcgcct tcgtggcgct ggcatcggcg cgcgtgctgc aacggcagcc ggtgctgtcg
     1561 gcgctgctgt ttgccgcgct gtggacgctg gccgagctgg cgcgcgggca atggctgacc
     1621 ggctttccgt ggggcgccgg cggttatgcg cacgtcgacg ggccgctcgc ctttctggcg
     1681 cgctacgtgg gcgtgtacgg catcggcttt gtggcggcgg cgctggcggc gctgctggcc
     1741 ttgagcggcc atgtgcgctg gcgctcgggg cgcacgctgg tcggcgccgc gctgctgctg
     1801 gcgctcggcg ccggcctgtg gggctggcgc ttttgcgatg tgaacctgtg ctacacgccc
     1861 gccgccggcc acgagcgctt ggtgaccgaa gtggcgctga tgcagggcaa catcccgcaa
     1921 gaccagaaat tcgtccccgg caccggcgtg gccgactcat tgcgctggta cggcgagcag
     1981 ttgcgcgacg tgcgcacgcc cctggtggtg gcgcccgaaa ccgcgctgcc gctgctgccg
     2041 cgccagttgc ccgaaggcta ctggcaggcg cttcttgatc gtttttcagg ccccaaccag
     2101 gccgcgctgc tgggcgtgcc gctgggcgac atggaggccg gctacaccaa ttcggtggtc
     2161 ggcatgaagg ccgggcaggc cgcgccctac cgctacgaca agcaccatct ggtgccgttt
     2221 ggcgaattca tcccgccgtt ctttcgctgg ttcaccgaga tgatgaacat tccgctgggc
     2281 gacttcacgc gcggcagcgt ggggcaagcg tcttttgaat ggcagggcca gcggctggcg
     2341 cccaacatct gctacgagga cttgtttggc gaagaactgg cagcgcgctt tgccaacccc
     2401 gccgcggcgc ccaccgcctt tgtcaacgtc agcaacatcg gctggtttgg cgacacggtg
     2461 gcgattgacc agcacctggc catcagccgc atgcgcgcgc tggagttcga gcggcccatg
     2521 ctgcgcgcca ccaacaccgg cgccaccgcc atcatcgacc accgggccac ggtcactcac
     2581 ctgctgccgc gcctgacgcg tggcgtgctg gtgggccatt tcgagggccg cacgaacatc
     2641 acgccgttcg cgcgctgggc cgccgtggcc gggctgtggc cgctgtggat cggctgcgcg
     2701 ctgctggtgc tggcctgcct ggcgttgcgg cgccaaagct gacgcggcgc aaggcgcgcc
     2761 gccttccccg tcaggcacaa ttgacgccat ggccgaatcc ttctcctacg atcaactcat
     2821 cgcctccggc gaaggccgct tgttcggccc cgacgccggt cgcctgccgc tgccgccgat
     2881 gctgatgttc gaccgcatca cgcacatcga cgccgatggc ggcgcgcatg gcctgggtcg
     2941 catcgaggcc gagctggacg tcaaccccga cctgtggttt ttcgcctgcc acttccaggg
     3001 cgacccggtg atgcccggct gcctggggct ggacgccatg tggcagctga tcggctttta
     3061 cctgacctgg ctgcgcctgc ccgggcgcgg gcgcgcactg ggcgcgggcg aggtcaagtt
     3121 caccggcgaa gtcggccccg atgtcaaacg ggtgacatac cagattgata tcaagcgggt
     3181 catcaagcgc aagctgaaca tggccattgg cgacgcgcgc ctgctggccg acggcaagga
     3241 gatctacgtg gccaatgacc tgcgcgtggg cttgttcctg cgcccgggcg aagggcagga
     3301 aagcagcgca tgagcaagca acgggtggtg atcacgggcg cgggcatcgt ctcgtgcatc
     3361 ggcaacgatc tggccagcgt ggaggcgtcg ctgcgcgaag gccgctccgg catccgcgcg
     3421 gtgcccgagt tcgcgcaact cggcctgcgc agccaggtcg gcggcaagcc cgagatcgac
     3481 atcgaggcgc gcatcgaccg caagcagctg cgcttcatgg gcgatgcggc ggcgtatgcg
     3541 cagatcgcgc tcgaagacgc gatcaagcaa gccggcctga cgccaaacca ggtcagccac
     3601 ccgcgcaccg gcctcatcat gggctcgggc ggcggctcgc ccgccaacca gatcgaagcg
     3661 gccgacacgc tgcgcgaaaa aggcattcgc cgcgtcgggc cgtaccaggt cacgcgctgc
     3721 atgagcagca cggtgtcggc gtgcctggcg accaacttca aggtcaaggg catcaactac
     3781 tccatcacct cggcctgcag cacgtcggcg cactgcatcg gcgcggccgc gcagcagatc
     3841 gcctggggcc atcaggacgt gatgtttgcc ggcggcggag aagagctgag ctggggcatg
     3901 gcgctgctgt tcgacggcat gggcgccatg tccgccaagt acaacgagac gcccgagaaa
     3961 gcctcgcgcg cctacgacgc cagccgcgac ggctttgtga ttgccggcgg cggcggcgcg
     4021 gtggtgctgg aatcgctggc gcatgcgcgg gcacgcggcg ccacgattct ggccgaggtg
     4081 gtcggctttg gcgccaccag cgacggcgcc gacatggtgg ccccctcggg cgacggcgcc
     4141 atcgcctgca tgcagcaagc gctggccggg ctggatggcg gcgtggacta catcaacacg
     4201 catggcacct ctacccccgt gggcgacatg caggaagtgc gcgccatgcg ggccgtgttt
     4261 ggcgacgcgg tgccgccgtt ctcttcaacg aagtcgctca ccggccattc gctgggcgcc
     4321 accggcgtgc aagaggcgat ctactgcctg atcatgctgc aaaagagctt catcgccggc
     4381 tcgatcaacg tcgacacgcc cgacccgtcg ctgggcgacc tgcccctggt cacgcaaacg
     4441 cgcgacgccg acgtgcgcac cgcgctgtcc aacagctttg gctttggcgg caccaacgca
     4501 agcctggtgc tgcgccgttt cgactcctga attcatagct gctcgcgctt gacagacaag
     4561 cgccagcggc c
//
LOCUS       DAOMVO010000197         4571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5386_length_4571_cov_14.353632, whole genome shotgun sequence.
ACCESSION   DAOMVO010000197 DAOMVO010000000
VERSION     DAOMVO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5386_length_4571_cov_14.353632"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKV30_09610"
     CDS             complement(<1..255)
                     /locus_tag="PKV30_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30502.1"
                     /translation="MSVDRVFKKKPAPALADQALAAPNQIANQGSAPLLPLGAMLLAG
                     SLGTSALAQSAATAADAPAAQQAPANAAPVAGTLPAVTVRD"
     gene            complement(499..978)
                     /gene="bfr"
                     /locus_tag="PKV30_09615"
     CDS             complement(499..978)
                     /gene="bfr"
                     /locus_tag="PKV30_09615"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRL30503.1"
                     /translation="MKGDMKAIGFLQAQLKNELTAINQYFLHYRMLKHWGLDKLAKKE
                     YEESIGEMKHADKLMDRIFMLDGLPNLQDLGKLMVGEDVPEILACDLKMEQGAQATIK
                     DGIAHCESVRDYVSRDLLQEILDDTEEHIDFLETQIELVGKVGLQNYLQSQMGEIAS"
     gene            complement(1120..>1321)
                     /locus_tag="PKV30_09620"
     CDS             complement(1120..>1321)
                     /locus_tag="PKV30_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL30504.1"
                     /translation="DPAFKQAVDGELKSMMASGEMAKHYDKWFMQPIPPRNVALNLPL
                     SDELKALFARPNDEPIESYNKR"
     assembly_gap    1322..1421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1452..3170
                     /locus_tag="PKV30_09625"
     CDS             1452..3170
                     /locus_tag="PKV30_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRL30505.1"
                     /translation="MSTEASIQIHPRKPRHQLKGRQATDAARAEVRALIGAAPPEGHR
                     RDLLIEHLHALNDHYHGLFERHLVALAAEMRLSMAEVYEVASFYHHFEVLKDDVMAPR
                     LTVRVCESLSCQMAGADALLAKLPALLGADVKVVPAPCIGRCEQAPAALVGQRGLGHA
                     TVEAIVSMSNRAVALSGQAPAAPETVVFDAYRQAGGYALAQAVACGERDAEGVIGGLE
                     HAGLRGLGGAGFPTGRKWRIVRGFEGPRLMAVNIDEGEPGTFKDRYYLERDPHRFLEG
                     VLIAAHVVGCDAVYLYLRDEYPECRAILTQAIAELQRLPELREQLPKIELRRGAGAYI
                     CGEESAMIESIEGKRGEPRLRPPYIAEVGLFGRPTLEHNFETLYWVREILERGPEWFA
                     SHGRHGRKGLRSFSVSGRVKEPGVKLAPAGITLRELVDEYCGGMQDGHTLYAYLPGGA
                     SGGIFPARLADVPLDFDTLQPHGGFIGSAAVIVLSQHDKARDAALNMMRFFADESCGQ
                     CTPCRVGTVKAAQLMEAPVWDAGTLDDLGTVMIDASICGLGQAAPNPMRCVQQYFAHE
                     VQEAAQ"
     gene            3167..>4571
                     /locus_tag="PKV30_09630"
     CDS             3167..>4571
                     /locus_tag="PKV30_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRL30506.1"
                     /translation="MSAVLKTNQPPVQADQAPAAIEFELDGRAVQAHVGETILQAAER
                     HGVEIPRLCYTEGLRADGNCRACVVEIEGERTLAPSCCRAVQPGMKVKATSERARKSQ
                     DMVLEMLLAEMPAEGFKWNDSLAPCPGGGRQGWGPTTAPTEAGSAPTPTLPQRGREQE
                     IGQHGELTQWAAQRTIHVRESLQNLKRAQPAPDLSHPAMAVNLSACIQCTRCVRACRE
                     AQVNDVIGYAGRGSHARIVFDLGDDMGASSCVACGECVQACPTGALMPKTQIGSQKVD
                     AQVDSVCPFCGVGCQITYNVRDAQIVSVSGRDGPANEKRLCVKGRFGFDYVASPDRLT
                     VPLIRKPGVTKDPAHCDDRSDWRAVFREATWDEALALAAGQLVDLKARHGPKSLAGFG
                     SAKGSNEEAYLFQKLIRTGFGSNNVDHCTRLCHASSVAALLEGVGSGAVSNPVKDVEF
                     AELILIIGSNPTANHPVA"
BASE COUNT          764 a         1422 c         1509 g          776 t
ORIGIN      
        1 atcgcgcacc gtgacggccg gcagcgtgcc ggccaccggc gccgcgttgg cgggcgcttg
       61 ctgcgccgcg ggcgcgtcgg ccgccgtcgc tgcggactgc gccagcgccg aggtgccgag
      121 cgagcccgcc agcagcatgg cgcccagcgg cagcagcggg gcgctgccct ggtttgctat
      181 ttgattggga gctgccagcg cttgatcagc aagcgctgga gcaggttttt tcttgaagac
      241 gcggtcgacg gacatggcgg ggcccagcta agtgatggaa aagaacttgg ctggctaccg
      301 agcgccaaaa ggcgggcgcg agaccctgcg gcgctgttcg cttgcgtgca atgaagctgc
      361 cgattttata tataaatgag aattattgtc attaactgtg tcgccgcgtt gcgacactgg
      421 ccaggcatcc gggcgagtga cggccaaaaa aaaagaggcc ttgcggcccc ttgttttcgt
      481 cacgcccatg caggcggttc agctggcgat ttcgcccatc tggctttgca ggtagttctg
      541 cagcccgacc ttgccgacca gctcgatctg cgtttcgagg aagtcgatgt gctcctcggt
      601 gtcgtccaga atctcttgca gcagatcgcg cgagacgtag tcgcgcacgc tttcgcagtg
      661 cgcgatgccg tccttgatgg tggcttgcgc gccttgctcc atcttcaggt cgcaggccag
      721 gatttcgggc acgtcctcgc ccaccatcag cttgccgaga tcctgcaggt tgggcaggcc
      781 gtcgagcatg aagatgcggt ccatcaactt gtcggcgtgc ttcatctcgc cgatcgattc
      841 ttcgtattcc ttctttgcca gcttgtccag gccccagtgc ttgagcatgc ggtagtgcag
      901 gaaatactgg ttgatggcgg tcagttcgtt cttgagctgc gcctgcagaa atccgatggc
      961 tttcatgtcg cctttcatgg cggtgctcct tgtacttttg tgcaaaaggc cattctaggg
     1021 agcgcgtgcg tcagcgccaa cctgcgggac tttcgtaatg cgaacgttta gcgtttcagc
     1081 agcgcgcgtg gcctgcgcgc gctgatggcg ctggcccgat cagcgcttgt tgtagctctc
     1141 gatcggctcg tcgttcggcc tggcgaacag cgccttcagc tcatccgaca agggcaggtt
     1201 gagcgccacg ttgcgcggcg gaatcggctg catgaaccac ttgtcgtagt gcttggccat
     1261 ctcgcccgaa gccatcatgc ttttcagctc gccatccacc gcttgcttga acgccgggtc
     1321 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacccagta tccaatgcgg
     1441 ataatgcgcc catgtccacc gaggcaagca tccagatcca cccgcgcaag ccgcgccatc
     1501 agttgaaggg gcgccaggcg accgatgccg cacgcgccga ggtgcgcgcg ctgatcggcg
     1561 cggcgccgcc cgaaggccat cggcgcgacc tgctgatcga gcatttgcac gcgctgaacg
     1621 accattacca cggcctgttc gagcgccacc tggtggcgct ggcggccgag atgcggctgt
     1681 cgatggccga ggtgtatgag gtggcgagct tctatcacca cttcgaagtg ttgaaggacg
     1741 atgtgatggc gccacgcttg acggtgcggg tgtgcgaatc gctcagttgc cagatggcgg
     1801 gcgccgatgc gctgctggcc aagttaccgg cgctgctggg ggccgacgtg aaggtggtgc
     1861 ccgcgccctg catcggacgt tgcgagcagg cgccggcggc gctggtgggg cagcgcggct
     1921 tggggcatgc cacggttgaa gcgattgttt caatgtcgaa tcgtgccgta gcgctttcag
     1981 gacaagcgcc agcagctcct gaaactgtag tgttcgatgc gtatcgccaa gccggcggct
     2041 atgccttggc gcaggctgtg gcgtgcggcg agcgcgatgc cgagggcgtg atcggcgggc
     2101 tggaacacgc cggcctgcgc ggcctgggcg gcgcgggctt tccgaccggg cgcaagtggc
     2161 gcatcgtgcg cggctttgag gggccgcgcc tcatggcggt gaacattgat gaaggcgagc
     2221 ccgggacttt caaggatcgc tattacctgg agcgcgaccc gcaccgcttt ctggaagggg
     2281 tgctgattgc cgcgcacgta gtcggttgcg acgcggtcta cctctacctg cgcgacgagt
     2341 accccgaatg ccgcgccatc ctgacgcaag ccattgccga gctgcagcgc ttgccagaac
     2401 tgcgcgagca gctcccaaaa atagagctgc gacgcggcgc cggcgcgtac atctgcggtg
     2461 aagaatcggc catgatcgag tcgatcgaag gcaagcgcgg cgagccgcgc ctgcgcccgc
     2521 cctacatcgc cgaagtcggc ctgttcggcc gcccgacgct ggagcacaac ttcgagacgc
     2581 tgtactgggt gcgcgaaatc ctggagcgcg gcccggagtg gtttgcatcg cacggccgcc
     2641 acggtcgcaa ggggctgcgc agtttcagcg tcagcgggcg cgtgaaagag ccgggcgtca
     2701 agctcgcgcc cgccggcatc acgctgcgcg agctggtcga cgaatactgc ggcggcatgc
     2761 aggacggcca cacgctgtac gcctacctgc ccggcggtgc ctcgggcggc atctttcccg
     2821 cgcggctggc cgacgtgccg ctggatttcg acacgctgca gccgcacggc ggcttcatcg
     2881 gcagcgcggc cgtcatcgtg ctcagccagc acgacaaggc gcgcgacgcg gcgctgaaca
     2941 tgatgcgttt cttcgccgat gaaagctgcg gccagtgcac gccgtgccgc gtcggcacgg
     3001 tgaaggcggc acagctgatg gaagcgccgg tgtgggatgc gggcactttg gacgacctgg
     3061 gcacggtgat gatcgatgcg tcgatctgcg gcctggggca ggcggcgccc aaccccatgc
     3121 gctgcgtgca gcagtacttc gcgcatgaag tacaggaggc ggcgcaatga gcgctgtttt
     3181 gaagacaaat caaccgccag tgcaggcaga tcaagcgcca gcagctatcg aatttgaact
     3241 ggatggccgc gctgtgcagg cgcacgtcgg cgaaaccatc ctgcaagccg ccgagcgcca
     3301 tggcgtcgag attccacgcc tttgctacac cgaaggcctg cgcgccgacg gcaactgccg
     3361 cgcctgcgtg gtcgagattg agggcgaacg cacgctggcc cccagttgct gccgcgccgt
     3421 gcagccgggc atgaaggtca aggcgacgag cgaacgggcg cgcaagtcgc aggacatggt
     3481 gctggagatg ctgctggccg agatgccagc cgagggcttc aagtggaacg actctttggc
     3541 tccttgcccc ggtgggggaa ggcagggatg ggggccgacc acggccccga cggaagcggg
     3601 gtcagccccc accccaaccc tcccgcaacg ggggagggag caagagatcg gccaacacgg
     3661 tgaactcacc caatgggccg cgcagcgcac catccacgtg cgcgagtcgc tacaaaacct
     3721 gaagcgcgca caacccgcgc ccgacctcag ccaccccgcc atggcggtga acctgtccgc
     3781 ctgcatccaa tgcacccgct gcgtgcgcgc ctgccgcgag gcgcaggtca acgacgtcat
     3841 cggctacgcc ggccgcggca gccatgcgcg catcgtgttc gatctgggcg acgacatggg
     3901 cgccagcagc tgcgtggcct gcggcgaatg cgtgcaggcc tgcccgaccg gcgcgctgat
     3961 gccgaaaacg cagatcggct cgcaaaaggt cgacgcccag gtcgattcgg tctgcccgtt
     4021 ctgcggcgtc ggctgccaga tcacctacaa cgtgcgcgat gcgcagatcg tcagtgtcag
     4081 cgggcgcgac ggcccggcca acgaaaagcg cctgtgcgtc aaaggccgct tcggctttga
     4141 ttacgtcgcc agcccggacc ggctgacggt accgctgatc cgcaagcccg gcgtgaccaa
     4201 agaccccgcg cattgcgacg accgcagcga ttggcgcgcc gtgttccgcg aagcgacgtg
     4261 ggatgaagcg ctggccttgg cggccggcca gctggtcgat ctgaaagcgc ggcacggccc
     4321 caaatcgctt gccggctttg gcagcgccaa gggcagcaac gaagaggcct acctgttcca
     4381 gaagctgatc cgcaccggct ttggcagcaa caacgtcgac cactgcacgc gcctgtgcca
     4441 tgcatccagc gtggcggcgc tgctcgaagg cgtgggctcg ggcgcggtga gtaacccggt
     4501 gaaggacgtc gagtttgccg agctgatcct catcatcggc tccaacccca cggccaacca
     4561 cccggtggcg g
//
LOCUS       DAOMVO010000198         4545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5429_length_4545_cov_13.666370, whole genome shotgun sequence.
ACCESSION   DAOMVO010000198 DAOMVO010000000
VERSION     DAOMVO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5429_length_4545_cov_13.666370"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="PKV30_09635"
     CDS             complement(<1..1374)
                     /locus_tag="PKV30_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRL30507.1"
                     /translation="MRRFSTPLIAAIFIAASAWWTCAAAQNNTQSEVVRTDQVQAKLL
                     AHAPQGVPIGQTPEAAAAQPVWVGLQITHAPEWHTYWKNAGDSGMPTELQWTLPPGVM
                     AGDIAWPLPKKIPIGHLANYGYEGTVLLPVPLIITPEYKPGVLADALDVKLKATWLVC
                     RKECIPQDGEFALKIPLRSSTALNGAAFDAALKSQPADVAGAHQVALKDDGKRLAVRV
                     AGLPVDVRGQPLELFPESPGVVVTAATPAKPGEPVGARSWSQHWDGDVWTADVPVSPE
                     RADSPEKMTLVLVAGERGWRAEAPVAGAWPALALPAGVSPALEAALKANANAGANAPP
                     PAAPTAVPATTFVWALLGALIGGLVLNLMPCVFPVLAIKVLGFTQHADDRRAHRTSGL
                     AYTAGVVLSFLALGALMLALRAAGQQLGWGFQLQSPAVVAALTVLFTVIGLNLAGVFE
                     FGSFLPSR"
     gene            1589..2845
                     /locus_tag="PKV30_09640"
     CDS             1589..2845
                     /locus_tag="PKV30_09640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRL30508.1"
                     /translation="MRLSTTTQQPDATLSPWAERLRQRVNVPLLVRWQGGRALPLGQF
                     DQPRVTMDVRDASGVAALLSPSLDHLGRAYVEGHVDIDGKAQDIIDVAWRLAEADAHQ
                     QAGGSGWLGRMAHRVADAVSHTRAQDSAAIQHHYDVSNAFYAEWLDPGMVYSCAYFEH
                     GDETLAEAQQKKIDHILTKLRLQPGQTLLDIGCGWGALVLRAASQFGARCVGITLSQK
                     QFELATERVRAAGLQDRVEIRLQDYRDVQGPFDRVSSVGMFEHVGLKNLASYFRIIHD
                     LLAPGGWALNHGITSTDPQNGATSYGGGDFIDRYVFPNGELAHIGTVLTTLQEGGLEA
                     VDIENLRRHYARTLQCWSDAFEAKTERLKQLVDEKTWRIWRVYLVGCQWAFEHDEIAL
                     YQILCRRAGQAPDGLPWSRRWVYGAA"
     assembly_gap    2901..3000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3001..3449)
                     /locus_tag="PKV30_09645"
     CDS             complement(<3001..3449)
                     /locus_tag="PKV30_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HRL30509.1"
                     /translation="MNSSHPCRRHALRLLASAAGAALLPAAWAQKRPESTAVELWKDA
                     SCGCCEDWITHMKQHGFTVTAHDTGNNAVRARLGLAQKYGSCHTALVGGYVVEGHVHA
                     DDVRRLLREKPVALGLAVPGMPVGSPGMDGAVYGGRRDKYDTLLVLR"
     gene            3575..4222
                     /locus_tag="PKV30_09650"
     CDS             3575..4222
                     /locus_tag="PKV30_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30510.1"
                     /translation="MTTPEHPAQHAAAPLPQARSAAADRLAALLTAACALLLGLLAAV
                     SLLALHLHFESRDRDLLHAHLQAARALLVRVDNTAALAALPARFEAAFADEPALAVRV
                     QGAYGQPLYEQGPASGLPPALLARPSASSQPAPLITWRSDDGRAWRGSALAMRLPMEG
                     ASTLTVAMALDIERHESFIASYRWALIGYVVLAALAFALLARWASGRALMKVNTP"
     gene            complement(4407..>4545)
                     /locus_tag="PKV30_09655"
     CDS             complement(4407..>4545)
                     /locus_tag="PKV30_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRL30511.1"
                     /translation="DLIDMQPCGGTHVANTAEIGRVVVTKIEKKSAQSRRVVLGWAAE
                     S"
BASE COUNT          681 a         1577 c         1516 g          671 t
ORIGIN      
        1 gcggctgggc agaaagctgc cgaactcgaa cacgccggcc agattcagcc cgatcacggt
       61 gaacagcacc gtcagcgccg ccaccacggc gggcgactgc agctgaaaac cccagcccag
      121 ttgctgcccc gccgcgcgca gcgccagcat cagcgcaccg agcgccaaaa acgacagcac
      181 cacgccggcg gtgtaggcca gcccgctggt gcggtgcgcg cgccggtcgt cggcgtgctg
      241 cgtgaagccc agcaccttga tcgccagcac ggggaatacg cagggcatca ggttcagcac
      301 cagcccgccg atcagtgcgc cgagcagcgc ccagacaaag gtggtggcgg gcacggcagt
      361 gggcgcggcg ggcggcggcg cgttggcacc ggcgttggca ttggccttca gcgcagcttc
      421 cagcgccggc gagaccccgg ccggcaaggc cagcgcaggc caggcgccgg ccaccggcgc
      481 ttcggcgcgc cagccgcgct cgccggccac caacaccagc gtcatttttt cggggctgtc
      541 ggcgcgttcg ggcgagacgg gcacgtcggc cgtccacaca tcgccgtccc agtgctggct
      601 ccagctgcgc gcgccgaccg gctcgcccgg cttggcgggc gtggcggcgg tgaccacgac
      661 gcccggcgat tcaggaaaca gctccagcgg ctggccgcgc acgtcgaccg gcaagccggc
      721 cacgcgcaca gcgaggcgct tgccatcgtc cttcagcgcc acctggtgcg cgcccgccac
      781 gtcggcaggc tggcttttca gcgccgcatc aaacgccgcg ccgttcaatg cggtggaact
      841 gcgcagagga atcttgaggg cgaattcgcc gtcctgcgga atgcattcct tgcggcacac
      901 cagccaggtc gccttgagct tgacgtcgag cgcgtcggcc agcacgccgg gcttgtattc
      961 gggcgtgatg atgagcggca ccggcagcag cacggtgcct tcgtagccgt agttggccag
     1021 gtggccgatg gggatttttt tcggcagcgg ccaggcgatg tcgcccgcca tcacgccggg
     1081 cggcagcgtc cactgcagct cggtcggcat gccggaatcg ccggcgttct tccagtaggt
     1141 gtgccactcg ggcgcgtgcg tgatctgcag gccgacccac accggctgcg cggcggcggc
     1201 ctcgggcgtc tggccgatcg gcacgccctg cggcgcgtgg gccagcagct tggcctgcac
     1261 ctggtcggtg cgcaccacct cggattgagt attgttttgg gccgcagcgc acgtccacca
     1321 agcgctggca gctatgaaaa tcgcagcgat cagcggggtg gagaagcggc gcatggcatt
     1381 cattggggca aacaggcaac gaggtgggag cgtggcgcgg gccggaggtt ccgcgattcg
     1441 cacggcaagg cgagcgtttg tcctacgccc tgcaccgtgc caatttcaaa caaacgtggt
     1501 aaatctgggc ttcaggtacc gatgcgcgaa gccgtgccgg cgcgcccgtc ggcttcattc
     1561 tcaccgcttg cctgtggagg cgccagtgat gcgattgtct accaccaccc aacagcccga
     1621 tgccacgctg tcgccgtggg ccgagcgcct gcgccaacgc gtgaacgtgc cgctgctggt
     1681 tcgctggcag ggcggccgcg cgctgcccct gggccagttt gaccagccgc gcgtcaccat
     1741 ggacgtgcgc gacgccagcg gcgtggccgc gctgctgtcg ccctcgctcg accacctggg
     1801 ccgcgcctac gtcgaaggcc atgtcgacat cgacggcaag gcgcaggaca tcatcgacgt
     1861 cgcctggcgc ctggccgagg ccgatgccca ccagcaggcc ggcggcagcg gctggcttgg
     1921 ccgcatggcg caccgcgtgg ccgacgccgt gagccacacg cgcgcgcaag acagcgcggc
     1981 cattcagcac cattacgatg tgtcgaacgc gttctatgcc gaatggctgg acccggggat
     2041 ggtttattcg tgcgcctatt tcgagcacgg cgacgagacg ctggcggaag cgcagcaaaa
     2101 aaagatcgac cacatcctga ccaagctgcg cctgcagccc ggccaaacgc tgctcgacat
     2161 cggctgcggc tggggcgcgc tggtgctgcg cgcggccagc cagtttggcg cccgctgcgt
     2221 cggcatcacg ctgtcgcaaa agcagtttga attggcgacc gaacgcgtgc gggccgccgg
     2281 gctgcaagac cgcgtcgaaa ttcgcctgca ggactaccgc gacgtgcaag gcccgttcga
     2341 ccgcgtcagc agcgtcggca tgttcgagca cgtggggctg aagaacctcg cgagctactt
     2401 ccgcatcatc cacgacctgc tggcgccggg cggctgggcg ctgaaccacg gcatcacctc
     2461 gaccgacccg caaaacggcg ccaccagtta tggcggcggc gacttcatcg accgctacgt
     2521 gttcccgaac ggcgaactgg cgcacatcgg caccgtgctg acgacgctgc aggaaggcgg
     2581 actggaagcg gtcgacatcg agaacctgcg ccgccactac gcgcgcacgc tgcaatgctg
     2641 gagcgacgcc ttcgaggcca agaccgagcg cctgaagcaa ctggtggacg agaagacctg
     2701 gcgcatctgg cgcgtctacc tggtcggctg ccagtgggcc tttgagcacg acgagattgc
     2761 gctctaccag atcctgtgcc gccgcgccgg ccaggcgcca gacggcctgc cgtggtcgcg
     2821 gcgatgggtg tacggagcgg cgtgacaagc gacttgcact gatttgatag cggcttgcgc
     2881 ttgtcagacg ggcgctgggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 tcgcgcagca ccagcagcgt gtcgtatttg tcgcgccggc cgccgtacac cgcgccatcc
     3061 atgccgggcg agccgaccgg catgcccggc acggccagcc ccagcgccac cggtttttcg
     3121 cgcagcaggc ggcgcacgtc gtcggcgtgc acatggccct cgaccacgta gccgccgacc
     3181 agcgcggtgt ggcacgagcc gtatttctgc gccagcccca gccgcgcgcg caccgcgttg
     3241 ttgccggtgt cgtgcgccgt caccgtgaag ccgtgctgct tcatgtgcgt gatccagtcc
     3301 tcgcagcagc cgcagctggc gtctttccac agttccacgg cggtcgactc gggccgtttc
     3361 tgcgcccacg cggcgggcaa cagggcagcg ccggcggcac tggccagcag ccgcagggcg
     3421 tggcggcgac acgggtgtga agaattcatg gcttgatctc ctggcaaaca aggtggtggc
     3481 ggcgcgcacg gcaccccggt tcaatcacgg gcacactcgc gcctttcagc cgcattgtcc
     3541 cgcagccgcc gcaccggcgc gggcgccacc gcccttgacc actcccgagc atccggcaca
     3601 gcacgccgcc gcaccgctgc cccaggcgcg atcggccgca gccgaccgcc tggcagcgct
     3661 gctgacggcc gcctgcgcgc tgctgctggg cctgctggcg gcggtgagcc tgctggcgct
     3721 gcacctgcac tttgagtcgc gcgaccgcga cctgctgcac gcccacctgc aggcggcgcg
     3781 cgcgctgctg gtgcgggtcg acaacaccgc cgcactggcc gcgctgccgg cgcgcttcga
     3841 ggccgcgttt gccgacgagc cggcgctggc cgtgcgcgtg caaggcgctt acggccagcc
     3901 gctgtacgag caagggccgg ccagcggcct gccgccggcg ctgctggcgc ggcccagcgc
     3961 cagttcgcag ccggcgccgc tcatcacctg gcgcagcgac gacggccgcg cctggcgcgg
     4021 cagcgcgctg gcgatgcgcc tgccgatgga aggcgcatcc acactcacgg tggcgatggc
     4081 gctggacatc gagcggcacg aatccttcat cgccagttat cgctgggcac tgatcggcta
     4141 cgtggtgctg gcggcgctgg cctttgcgct gctggcgcgc tgggcgtcgg gtcgcgcgct
     4201 aatgaaagtc aacaccccct gagccgcctt cggcgtcttc cccctctctc tgcggcaggg
     4261 ggacggcgcc actggccggg gaaaccccgg ccacggcgcc cacgcatggc ctgctccgcg
     4321 gccatccgat gccgtccgct gtcgagacga catcaaaatt catagcggct cgcgcttgtc
     4381 catcaagcgc tggcggcact tttcttttaa gactcagccg cccagcccag caccacgcgg
     4441 cggctttgcg cgcttttctt ttcgatcttg gtgaccacca cgcggccgat ctctgccgtg
     4501 ttggccacgt gcgtgccgcc acagggctgc atgtcgatca agtcg
//
LOCUS       DAOMVO010000199         4512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5486_length_4512_cov_12.802558, whole genome shotgun sequence.
ACCESSION   DAOMVO010000199 DAOMVO010000000
VERSION     DAOMVO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5486_length_4512_cov_12.802558"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKV30_09660"
     CDS             <1..972
                     /locus_tag="PKV30_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRL30512.1"
                     /translation="MVTKKPKGLGRGLDALLGPTLASSGEGDAATPAGTPMMLALDAM
                     APGQYQPRTRMDEGALYELAESIKAQGIMQPILVRRLQSKAASAQAGQARAATDFAAN
                     YEIIAGERRFRAAKLAGLTEVPVLLREVPDEAAAAMALIENIQREDLNPLEEAQGIRR
                     LTADFGLTHEQAAQAVGKSRSAASNLLRLLNLTEPVQTMLMAGDIDMGHARALLALDG
                     AAQITAANQVSAKKLSVREAESLVKKLSAEFNLTAPRKPARSADKSGDVRRVEEELSD
                     LLTAQVEVRVKKRGGRGAAQAGEVAIQFGSLDELNGLIERLRASGGR"
     gene            1060..1809
                     /locus_tag="PKV30_09665"
     CDS             1060..1809
                     /locus_tag="PKV30_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRL30513.1"
                     /translation="MALRLTKPRKPRNPGAPALARGVRRGGDGPALAVRAARASVERV
                     RRAVDLAVKRSPQSLFLRELVAQPGTVGAICASSPRLAARMAAWVDATAPGLVVELGG
                     GTGVITAALLARGVAPERLVVIEQSAALAAHLRRRFAQIHVVHGDAAELVLLAQSAGW
                     PRDGAGGPLPVHCIVSGLPLLSIPPAVRQRILHAGAAVLAPHGRLLQFTYAMRGPSAW
                     QAAGLVGQARERVLANLPPARIDVLGHDVRG"
     gene            1992..2693
                     /locus_tag="PKV30_09670"
     CDS             1992..2693
                     /locus_tag="PKV30_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30514.1"
                     /translation="MKSSHKNAFVHSLRAAALLGGLLVGASVQAGMVTDRHGNVGYDT
                     AAECDAAVASGQARYYQPVTVGKPVRRAGEASVRQMPMRQLAQLGATPAGALGYSSAG
                     YAHGACDLGAPSVGGREHVTPPLVGKYVPFSPDMMVNVFYDRQGQPRRVSMAQCDNSF
                     QAAWPRPVAVTPVAVTPPPAAPVACYVDALRPIVPVATPGCGEGMRCHQLLPLPPSKL
                     PGIERAGGAQACTNS"
     gene            2852..3604
                     /locus_tag="PKV30_09675"
     CDS             2852..3604
                     /locus_tag="PKV30_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30515.1"
                     /translation="MKRSLRIGAMVLAAGLATLTGCATKSPIPLAENFDLTVQPKVRS
                     AGHWDLLSKDVVTQTLASLRASQIPADTPMYVGLPANPSQFDVGFREFLITKLVQNGQ
                     SVYMQPSQAASVALQTEACTAPIACRTTDALEVTYQTQVVRHDSPRPHFVPHQFTIIT
                     AGLMAAYGLRFEHLDTKLIAGLGLAAAADYAASVSSGGPTNTELILTTTVSNRGRFVS
                     RKTDVYYLENVDAPLFMRSAPAFKPVNLRVVS"
     gene            3604..4167
                     /locus_tag="PKV30_09680"
     CDS             3604..4167
                     /locus_tag="PKV30_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HRL30516.1"
                     /translation="MKRAILLSVIAVALSGCASNEPPSSRRAGPTYEAASQNAFLDSG
                     RTAVVKLVEGLPPRALADGPMLVSTIVNVNNLTRSAPLGRTLSEQYASHLSSLGFNVK
                     EVKLRGDLFVREGTGELLLSRELRDIARSHNAAVVLVGTYSPATDYTYVSVKLVRTED
                     SRILRGYDYALPNDRDVQRLLQDPVFR"
BASE COUNT          721 a         1427 c         1515 g          849 t
ORIGIN      
        1 atggtcacca agaaacccaa aggcctcggc cgcggcctcg acgcgctgct ggggcccacg
       61 ctggcctcct ctggcgaggg cgatgccgcc acgccggccg gcacgccgat gatgctggcg
      121 ctggacgcca tggcgcccgg ccagtaccag ccgcgcacgc gcatggacga gggcgcgctg
      181 tacgagctgg ccgagtcgat caaggcgcag ggcatcatgc agccgatcct ggtgcggcgt
      241 ttacagtcaa aagcggcctc agcgcaggcg gggcaagcgc gagcagctac tgattttgca
      301 gcaaactacg aaatcatcgc cggcgagcgc cgctttcgcg ccgccaagct ggccggcctg
      361 accgaggtgc cggtgctgct gcgcgaggtg cccgacgaag ccgccgccgc catggcgctg
      421 atcgagaaca tccagcgcga agacctgaac ccgctggaag aagcgcaggg catccgccgc
      481 ctgacggccg actttggcct gacgcacgag caggccgcgc aggccgtcgg caaaagccgc
      541 agcgccgcca gcaacctgct gcgcctgctc aacctgaccg agccggtgca aaccatgctg
      601 atggccggcg acatcgacat gggccacgcg cgcgcgctgc tggcgcttga tggcgcggcg
      661 cagatcacgg cggccaacca ggtcagcgcg aaaaagctca gcgtgcgcga ggccgaatcg
      721 ctggtcaaga agctcagcgc cgaattcaac ctgaccgcgc cgcgcaagcc ggcccgcagc
      781 gccgacaaat cgggcgacgt gcggcgggtg gaggaagagc tgtcagacct gctcaccgcg
      841 caggttgagg tgcgcgtgaa aaaacgcggc gggcgcggcg cggcgcaggc tggcgaggtg
      901 gcgattcagt tcggctcgct ggacgagctg aacggcctca tcgagcggct gcgtgccagt
      961 ggcggccggt gagcccgccc ggcgcttggt tacatctgtt gatgtgcgcg gtataagctc
     1021 tcgtctttcg ttttccgtcc ctaacgtact gattcgctca tggcgctgcg cttgaccaag
     1081 ccccgcaagc cccgcaatcc gggcgcgccc gctttggcac gtggtgtgcg gcgtggcggc
     1141 gacgggccgg cgctggccgt gcgcgcggcg cgcgccagcg tcgagcgtgt gcggcgggcg
     1201 gtcgatcttg ccgtcaagcg ttcgccgcaa tcgctgttct tgcgcgagct ggtggcacag
     1261 ccgggtacgg tcggtgccat ctgcgcttct tcgccccggc tggccgcgcg catggcggcg
     1321 tgggtcgatg cgacggcgcc ggggctggtg gtggaattgg gcggcggtac cggcgtcatc
     1381 accgcagcgc tgctggcccg cggcgtggcg ccagagcggc tggtggtgat cgagcagtcg
     1441 gcggcgctgg cggcgcattt gcggcgcagg tttgcgcaga tccatgtggt gcatggcgat
     1501 gccgccgagc tggtattgct ggcgcagtcg gccggctggc cgcgtgacgg cgcgggtggg
     1561 ccgctgccgg ttcactgcat cgtttctggg ctgccgctgt tgtcgattcc gccagccgtg
     1621 cgccagcgca tcctgcacgc cggcgccgcg gtgctggcgc cccacgggcg attgttgcag
     1681 ttcacctacg ccatgcgcgg gccttcggcc tggcaggccg cgggcctggt cggtcaggcc
     1741 cgcgagcgcg tgctggccaa cttgccgccg gcgcggatcg acgtgctggg gcacgatgtg
     1801 cgcggctgaa cagtgcgcta gaacggccgg cggccgctac ccggcgcgcc acggcaagtt
     1861 cgaccgctgt gttgtgtttc acgcgtaaat gctggtcgga ctgccctttt ttgtgtcacg
     1921 tggagttacc atttgagctt cgagccgtcc gcccacgtga tgggcgcgcc ccattctgga
     1981 ggaaccacgc catgaagtcg tcgcataaga acgctttcgt ccattcactt cgcgctgccg
     2041 cgctgttggg cggcttgctg gtcggcgcgt cggtgcaggc cggcatggtc accgatcggc
     2101 acggcaacgt cggctacgat acggccgccg agtgcgacgc ggcggtggcg tcgggccagg
     2161 cgcgctatta ccagcccgtg accgtgggca agcccgtgcg gcgagctggc gaggcgtcgg
     2221 tgcgtcagat gccgatgcgc cagttggccc agctcggtgc cacgccggcc ggcgcgctgg
     2281 gttattccag cgcgggctat gcgcatggtg cgtgcgatct gggcgcgccg tcggtgggcg
     2341 gccgcgagca cgtcacgccc ccattggtgg gcaaatatgt gcccttcagc ccggacatga
     2401 tggtgaacgt cttctacgat cgccagggtc agccgcgccg tgtctcgatg gcgcaatgcg
     2461 acaacagctt tcaggccgca tggccgcgcc cggtagcggt gacgccggtg gccgtcaccc
     2521 cacccccggc ggcgcctgtt gcgtgctatg tggatgcgtt gcgtcccatt gtgccagtgg
     2581 ccacgccggg ctgcggcgaa ggcatgcgat gccaccaatt gctgccgctg ccgccgtcaa
     2641 aactgccggg tattgaacgt gccggcggcg cgcaggcgtg caccaactcg tgagcgatcg
     2701 ccggcggatg gtgagcaaaa acaacaaatc gctgcttctt tatgcactta gcgctgcttg
     2761 ttgttgtgtt ggcttaacag tctgcgcaga atgtaataaa acattttcaa agcagccgtg
     2821 gtgttgcgat ttttatgtct gaggggaaat catgaaacgt tcgttgcgca tcggtgccat
     2881 ggtgctggcg gcgggtttgg cgactctgac gggttgcgcc accaaatcac ccattccgct
     2941 ggccgaaaat ttcgatttga ccgttcagcc caaggtgcgc tcagccggcc actgggatct
     3001 gttgtccaag gatgtggtga cacaaacctt ggcgtcgctg cgcgccagcc agattccggc
     3061 tgacacgccc atgtacgtgg ggctgccagc caatccctcg caatttgatg tggggtttcg
     3121 tgagtttctg atcaccaagc tggttcagaa cgggcagtct gtttacatgc agcctagcca
     3181 agcagcctcg gtcgcactgc aaaccgaggc gtgcacggcg cccatcgcct gccgcacgac
     3241 cgacgcgctg gaagtgacct accaaaccca ggtggtgcgc catgacagcc cgcgtccgca
     3301 tttcgttccg caccaattca ccatcatcac ggccggcctg atggcagcct atggtttgcg
     3361 gtttgagcat ctcgacacca aacttatcgc cggcctgggt ctggcggccg ctgcagacta
     3421 cgccgcgagc gtcagcagtg gcggcccgac gaataccgaa ctcatcttga ccaccacggt
     3481 cagcaaccgc gggcgtttcg tgagccgaaa aaccgacgtt tactaccttg agaacgtcga
     3541 tgcgccgctg ttcatgcgct cggcccctgc gttcaagccc gtcaacctgc gggtggtgtc
     3601 atgatgaagc gcgctatcct cctgtcggtc attgctgttg cgctgtcggg ctgcgcctcc
     3661 aatgagcccc cgtcttcccg gcgcgctggg cccacgtacg aagccgcatc gcagaacgcg
     3721 tttctggaca gcggccgcac cgctgtagtc aagctggtgg aggggttgcc tccccgcgcg
     3781 ctcgctgacg gccccatgct ggtgtcgacc attgtcaacg tcaacaacct gacgcgttcc
     3841 gcgccgctgg gtcgcacgct gtctgagcaa tacgcttcgc acttgtcgtc gcttggcttc
     3901 aatgtgaagg aagtgaagct gcgtggcgat ctgtttgtgc gcgaaggcac gggcgaattg
     3961 ctgttgtcgc gcgaactgcg cgatatcgcg cgcagccaca atgcagctgt cgtgctggtc
     4021 ggcacctact cgcccgcgac cgattacacc tacgtgagtg tcaagctcgt gcgcaccgaa
     4081 gacagccgca tcctgcgcgg gtacgactat gcgttgccca atgaccgcga cgtccaacgc
     4141 ctgctacagg acccggtttt ccgctgacct ttgagagggc tgtaatcagc cgcacgcctg
     4201 atttgtcaaa tttgcttcag gcgttttttg cattagttga aggggccagg tgttggattt
     4261 caatcaaaca cctcccatgg cttcttgacg ccattcgacg cggcgggatg gcaaggtagc
     4321 actttgcctt tatgatcggc tccttgtgtt gtggcggtcg aagagcgtgg gaatgcgtgc
     4381 ggcccctacc aaagtagggt atgggcgatt tgcgcgacgt ggcacgatcc agcggctatt
     4441 tttgttgtgc cgtattagcg gcctgaaccc tggagttgat cggatgtcat cgaagacgaa
     4501 actggccgtg ct
//
LOCUS       DAOMVO010000200         4508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5489_length_4508_cov_18.088929, whole genome shotgun sequence.
ACCESSION   DAOMVO010000200 DAOMVO010000000
VERSION     DAOMVO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_4508_cov_18.088929"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..200)
                     /locus_tag="PKV30_09685"
     tRNA            complement(126..200)
                     /locus_tag="PKV30_09685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Thr,seq:ggt)
     gene            complement(229..302)
                     /locus_tag="PKV30_09690"
     tRNA            complement(229..302)
                     /locus_tag="PKV30_09690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Gly,seq:tcc)
     gene            complement(339..424)
                     /locus_tag="PKV30_09695"
     tRNA            complement(339..424)
                     /locus_tag="PKV30_09695"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Tyr,seq:gta)
     gene            complement(441..1232)
                     /locus_tag="PKV30_09700"
     CDS             complement(441..1232)
                     /locus_tag="PKV30_09700"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRL30517.1"
                     /translation="MSESSLHEGAAAPSLSAWPPKPPQVAPGWQPLLQEFLQSADGQR
                     VADRLQADLSAGTAVFPPDPLRALRLTAPEDVRVVILGQDPYHGPGQAYGLAFAVPPG
                     VRMPPSLRNIHKELQRDFGRAPEPNGLLEHWARQGVLLLNTSLTVASGQAGSHGKIGW
                     QALTGSVFQRLVESKRPLAFMLWGGHAQAMRPQTDTAKRHLWLLANHPSPLSALRPPV
                     PFIGCGHFGRANDFLRRHGDPPIDWLGEALPDAPTPLVGPGQLVA"
     gene            complement(1290..2102)
                     /gene="trpC"
                     /locus_tag="PKV30_09705"
     CDS             complement(1290..2102)
                     /gene="trpC"
                     /locus_tag="PKV30_09705"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRL30518.1"
                     /translation="MSDILEKIVAVKREEVAAGLKKKSLAAMREDAESRVLTRDFEGA
                     LRAKIAQGQAAVIAEIKKASPSKGVIRPDFEPADIAQSYAVGNTDAGGKVSAACLSVL
                     TDKQFFQGSIDYLKQARASCELPVLRKDFMIDPYQVYEARAIGADCILLIAACLGDGQ
                     MNELEAVAHALGMAVLVEVHDGAELQRALKLKTPLVGINNRNLRTFDVTLDTTLGLLK
                     EVPADRLLITESGIANRADVQRMRDSGVHAFLVGETFMRADEPGEALAELFS"
     gene            complement(2095..2238)
                     /locus_tag="PKV30_09710"
     CDS             complement(2095..2238)
                     /locus_tag="PKV30_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30519.1"
                     /translation="MWQDEGTWIALGVSALFLVAALVMHRVFKKILKGPPPDQREQLS
                     KDE"
     gene            complement(2238..3275)
                     /gene="trpD"
                     /locus_tag="PKV30_09715"
     CDS             complement(2238..3275)
                     /gene="trpD"
                     /locus_tag="PKV30_09715"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRL30520.1"
                     /translation="MTITAQEALQRTIEHREIFHDEMLHLMRMIMKGEISPLMMAAIT
                     TGLRVKKETIGEITAAAQVMRELSTKVHVADPTHLVDVVGTGGDGSHTFNISTCSMFV
                     AAAGGARVAKHGGRSVSSKSGSADVIEALGIALNLTPEQIAKSIAETGVGFMFAPNHH
                     PAMKNVAPVRKEMGVKTFFNILGPLTNPAGAPNIVMGVFHPDLVGIQVRALQRLGAEH
                     ALVVYGRDGMDECSLGAATMVGELKDGQISEYDIHPEDFGMQMQSNRAIKVETPEQSL
                     AMLKGVLDNQDGPAKEIVVLNAGLVLYASGVASTMKAGMDRAREAIEAGAAKAKLEQV
                     LAFTQSLPKAA"
     gene            complement(3309..3989)
                     /locus_tag="PKV30_09720"
     CDS             complement(3309..3989)
                     /locus_tag="PKV30_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRL30521.1"
                     /translation="MPDFHHLLLFIAAGLLLNLTPGPDVMFIVANAVRAGARAGVAAA
                     LGISAGCLVHVAAAALGVSALLAASRAAFGVLKWVGAIYLVWVGVQMLRSTARPNASI
                     NIAARACQTSVDGPFHAQTPLATVFRRGFLTNVLNPKVALFFLAFVPQFIAPGTAQTA
                     WVFLALGLLFTVNGLLVCIGWALAAAWAARRAGALQRATRWLDGVAGGLFIAFGIKLA
                     LTDVPGGR"
     gene            complement(3996..>4508)
                     /locus_tag="PKV30_09725"
     CDS             complement(3996..>4508)
                     /locus_tag="PKV30_09725"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRL30522.1"
                     /translation="VTVLRNDEVTLEELDAMFMRGEFDRLCISPGPCSPNEAGVSVAA
                     ITHFAGKLPILGVCLGHQAIGAAFGGQIVRAKTQMHGKTSVITTTQQGVFADLPAQFT
                     VNRYHSLVIEKESCPKELEITATSDDDGEIQGVRHTGFAHDVRIEGVQFHPESILTEH
                     GHAMLANFLN"
BASE COUNT          802 a         1494 c         1425 g          787 t
ORIGIN      
        1 tggtgccgac gttcacgtgc ggcttggtcc gctcgaattt ctcttttgcc atttcttcga
       61 ctccgaaaaa aagtgactca acaacacacg aaaagcagca gcgccacatg gcgcgccacc
      121 ttgattggtg cccttggtgg gaatcggacc cacgacctct cccttaccaa gggagtgctc
      181 taccactgag ccacaagggc gaaaacctca aagccagtcg accacgaatg gagcgggaga
      241 cgggaatcga acccgcgtca ttagcttgga aggctagggt tctaccattg aactactccc
      301 gcgaaccgct gcgcactgcc gcgaccgact ctttctgctg gtggagaggg ctggattcga
      361 accagcgtac tcgtaagagg gcagatttac agtctgctgc cattaaccac tcggccacct
      421 ctccggcaga gaaccacgga ttatgccacc aattgaccgg ggccgacaag tggtgtaggg
      481 gcgtcgggca gcgcttcgcc cagccagtcg atgggcggat cgccgtgccg acgcaagaag
      541 tcgttggcgc ggccaaagtg cccgcagccg atgaaaggca ccggcgggcg cagcgccgac
      601 agcggcgacg ggtggttggc caaaagccac aaatgccgct ttgccgtgtc ggtttgaggg
      661 cgcatcgcct gcgcgtggcc tccccacagc atgaacgcta gcggacgctt actttcgacc
      721 aaacgctgaa aaacagagcc cgtcagcgcc tgccagccga ttttgccgtg actgccggcc
      781 tggcccgacg ccaccgtgag gctggtgttc agcagcaaca cgccctgacg cgcccagtgc
      841 tccagcagcc cgttcggctc cggtgcgcgg ccgaagtcgc gctgcagctc tttgtggatg
      901 ttgcgcaggc tcgggggcat cctgacgccc ggcggcaccg caaaagccaa gccgtaagcc
      961 tgacccgggc cgtggtaagg gtcctggccg aggatcacga cgcgcacgtc ttccggcgcg
     1021 gtcagccgca gggcgcgcag cgggtcgggt ggaaacacgg cggtgccggc ggacagatcg
     1081 gcctgcagcc gatccgccac ccgttggccg tcggcactct gcagaaattc ctgcagcagc
     1141 ggctgccagc ccggcgccac ctgaggcggc ttgggtggcc aggcgctcaa cgagggggcg
     1201 gcagcgcctt catgcaacga tgactcagac atcaaatagg ccgccagcgc ttgctggaca
     1261 agcgcaagca gctatgaaaa acatagtttt caggaaaaca actccgccag cgcctcgccc
     1321 ggctcatccg cccgcatgaa ggtctcgccg accagaaacg cgtgcacgcc tgagtcgcgc
     1381 atccgctgca catcggcgcg gttggcgatg ccactttcgg tgatcagcag gcgatcggcc
     1441 ggcacctcct ttagcaagcc cagcgtggtg tcaagcgtga cgtcaaacgt gcgcaggttg
     1501 cggttgttga tgccgaccag cggcgtcttc agcttgagcg cgcgctgcag ctcggcgcca
     1561 tcatgcacct cgaccagcac agccatgccc agcgcgtgcg ccaccgcttc cagctcgttc
     1621 atctggccgt cgcccaggca ggcggcgatc agcagaatgc aatcagcccc gatggcgcgc
     1681 gcctcataca cctggtacgg gtcgatcatg aaatccttgc gcagcaccgg cagctcgcaa
     1741 ctggcgcgcg cctgcttcag gtagtcgatg ctgccctgaa agaactgctt gtcggtcagc
     1801 accgacaaac acgccgcgct gaccttgccg cccgcatcgg tgttgcccac ggcgtagctt
     1861 tgcgcgatgt cggccggctc gaagtcgggg cggatcacgc ccttgctcgg gctggccttc
     1921 ttgatctcgg cgatcacggc cgcctggcct tgggcgatct tggcgcgcaa ggcgccctcg
     1981 aagtcgcgcg tcagtacacg gctttcggcg tcttcgcgca tggcggcgag cgacttcttc
     2041 ttcagccccg cggcgacttc ctcgcgcttg acggcgacta ttttttcaag gatatcactc
     2101 atctttcgat agctgctcgc gctggtctgg cggcggcccc ttcagtattt tcttgaaaac
     2161 ccggtgcatg acgagcgccg cgaccagaaa cagcgccgag acgcccaggg cgatccaggt
     2221 gccttcgtcc tgccacatca ggccgccttg ggcagcgatt gggtaaacgc cagcacctgc
     2281 tccagcttgg ccttggcggc gccggcttcg atggcttcgc gcgcacgatc catgcccgcc
     2341 ttcatcgtac tggccacgcc ggaagcgtag agcaccaggc cggcgttcag caccacgatc
     2401 tccttggccg ggccgtcctg gttgtcgagc acgcccttca gcatggccag cgactgctcg
     2461 ggcgtctcga ccttgatggc gcggttgctc tgcatctgca tgccgaagtc ttcggggtga
     2521 atgtcgtact cgctgatctg cccgtctttc agctcgccca ccatggtcgc ggcgcccagg
     2581 ctgcattcgt ccatgccgtc gcgcccgtac accaccagcg cgtgctcggc gcccaggcgc
     2641 tgcagcgcgc gcacctgaat gcccaccaga tcggggtgaa acacgcccat cacgatgttg
     2701 ggcgcgcccg ccgggttggt gagcgggccg aggatgttga agaaggtttt gacgcccatc
     2761 tccttgcgca ccggcgcgac gttcttcatc gccgggtggt ggttgggcgc gaacatgaag
     2821 cccacacccg tctcggcgat gcttttggcg atttgctcgg gcgtgaggtt gagcgcaatg
     2881 cccagcgcct cgatcacatc cgcgctgccc gacttgctcg acacgctgcg cccgccgtgc
     2941 ttggccacgc gcgcgccgcc ggcggcggcc acgaacatgc tgcaggtgct gatgttgaag
     3001 gtgtgcgagc catcgccgcc cgtgcccacc acgtcgacca ggtgcgtcgg gtcggccacg
     3061 tgcaccttgg tcgacaactc gcgcatcacc tgcgcggcgg cggtgatctc gccaatcgtc
     3121 tccttcttga cgcgcaggcc ggtggtgatg gcggccatca tcagcggcga gatttcgcct
     3181 ttcatgatca tgcgcatcag gtgcagcatt tcgtcatgaa agatctcgcg gtgctcgatg
     3241 gtgcgttgca gggcttcctg ggcggtgatg gtcatggtga aaatctctct ttgaaaatga
     3301 atcggtcgtc agcgcccgcc gggcacatcg gtcagcgcca gcttgatgcc aaacgcgatg
     3361 aacagcccgc ccgccacgcc atccagccag cgcgtggcgc gctgcagcgc ccccgcgcga
     3421 cgtgccgccc aagcggcggc cagcgcccag ccgatgcaga cgagcaagcc gttgacggta
     3481 aacagcagcc ccagcgccag aaacacccac gccgtctgcg cggtgccggg cgcgatgaac
     3541 tgcggcacaa aggccagaaa gaacagcgcc accttggggt tgagcacgtt ggtcagaaag
     3601 ccgcgacgga acacggtcgc cagcggcgtt tgagcatgaa atgggccgtc aacgcttgtc
     3661 tggcaagcgc gagcagctat gtttatagaa gcatttggac gcgcggtcga gcgcagcatc
     3721 tgcacgccca cccacaccag atagatcgcg cccacccact tcaacacgcc aaacgccgca
     3781 cggctggccg ccagcagcgc cgacacgccc agcgccgcag cagcgacgtg caccagacag
     3841 ccggcagaaa tccccagcgc cgccgccaca cccgcgcgtg caccggcacg caccgcgttg
     3901 gcgacgatga acatcacgtc cgggccgggc gtcaggttga gcagcaggcc ggcggcgatg
     3961 aagagcagca ggtggtgaaa gtccggcatg gcaactcaat tcaggaagtt ggccagcatc
     4021 gcgtgcccat gctcggtcag gatgctctcg gggtggaact ggaccccttc gatgcgcacg
     4081 tcgtgggcga agccggtgtg gcgcacgccc tgaatttcgc cgtcgtcgtc gctggtggcg
     4141 gtgatttcca gctctttcgg gcagctttcc ttctcgatca cgagcgagtg gtagcggttg
     4201 acggtgaact gcgcgggcaa atcggcgaac accccttgct gcgtcgtggt gatgacgctg
     4261 gtcttgccgt gcatctgcgt tttggcgcgc acgatttgac cgccgaaagc cgcgccgatg
     4321 gcctggtggc ccaggcacac gcccaggatg ggcagcttgc cggcgaagtg tgtgatggcg
     4381 gcgaccgaca cgccggcctc attgggcgag caggggccgg ggctgatgca gaggcggtcg
     4441 aattcgccgc gcatgaacat ggcgtcgagt tcttccagtg tgacttcgtc attgcgcagc
     4501 acggtcac
//
LOCUS       DAOMVO010000201         4499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5507_length_4499_cov_11.327633, whole genome shotgun sequence.
ACCESSION   DAOMVO010000201 DAOMVO010000000
VERSION     DAOMVO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_4499_cov_11.327633"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PKV30_09730"
     CDS             <1..1358
                     /locus_tag="PKV30_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRL30523.1"
                     /translation="GAGTERLIPFDLIPRIIPADEWDMLERGLTQRVTALNRFIHDVY
                     HEQEILRAGIVPSDQVLRNAQYRAIMKGVAVPNDVYSHIAGVDIVRAGTEGGKGEYYV
                     LEDNLRVPSGVSYMLEDRKMMMRLFPGLFSQYRVAPVAHYPDLLLETLRDSSPAATAN
                     PTVVVLTPGMYNSAYFEHAFLAQQMGVELVEGQDLFVKDDFVYMRTTRGPKRVDVIYR
                     RVDDDFIDSQVFRPDSALGCAGLMRAYRAGHVTICNAVGTGVADDKSIYPYVPKMIEF
                     YLGEKPILNNVPTYMCRDADDLKYVMANMQDLVVKEVHGAGGYGMLVGPASTAAEVDE
                     FRKAVLARPDGYIAQPTLSLSSCPTYVESGVAPRHIDLRPFVLSGKTVQMVPGGLTRV
                     ALKKGSLVVNSSQGGGTKDTWVLEESSASGARRSVVEQIQGPTSEQVQRSAFGVQRQS
                     F"
     gene            1415..2371
                     /locus_tag="PKV30_09735"
     CDS             1415..2371
                     /locus_tag="PKV30_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRL30524.1"
                     /translation="MLSRTADHLFWMSRYTERAENTARMLNVHHETSLLPQSAAVSQY
                     GWLGVLSLSELLPAYTERQGEVSPRGVMRFMVLDEENPSSIVACLKAARENARAVRGT
                     LTTEVWETLNQTWLEMSRLARSGDFEEDPAEFFEWVKFHSHLSRGVTLGTMLQDEAFH
                     FLRLGTFLERADNTARLLDVKFHAVESEFFGTASEKDLEYDFYHWAAILRSVSAFEIY
                     RKVYRDVIVPERVAELLMLREDMPRALHHCMSEVLTNLRLVGAEPQGETMRHAGRLRA
                     DLRYGHIDEILATGLHAFLTQFLDRVNQLGGHVSRDFLVPAG"
     gene            2485..2769
                     /locus_tag="PKV30_09740"
     CDS             2485..2769
                     /locus_tag="PKV30_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30525.1"
                     /translation="MKSERITFLATPEFKANLTQLAVRENTSVGEVIRGRFERAQNRP
                     DASEESELRALVADLRAATAKAQTSLSEGLAEAEQTLRELRKSSQRKAAA"
     gene            2766..2969
                     /locus_tag="PKV30_09745"
     CDS             2766..2969
                     /locus_tag="PKV30_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30526.1"
                     /translation="MSALTDAAKAIKEVLKLTDDVKRAGEALKDISRELREHDRRITR
                     LEAKWDTAMQLSATRPLPPTDRA"
     gene            complement(3032..4129)
                     /gene="mltB"
                     /locus_tag="PKV30_09750"
     CDS             complement(3032..4129)
                     /gene="mltB"
                     /locus_tag="PKV30_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HRL30527.1"
                     /translation="MTDFTAFNRRDVLATLCIAAGALPASAGAQNRSRRPRAERAPDI
                     PYAERAEAMRFADDLAARRGLPADWVRSAIGQAVYLPNVPRLMLPPARGTAKNWRVYR
                     SRFIDPVRIRAGARFWERNRTTLARAQAEFGVPPEIIVGIVGVETIYGQNMGSFRVID
                     ALATLTFDFPPQHPRAQARQAFFQDELEQFLSLSYRTGMDPLQPVGSYAGAMGMPQFM
                     PSSWVKYAIDYDGDGRIDLWNSEADVIGSVANYFKGYGWQPGVPTHYGVRLAPEAQMD
                     ALLAPDILPSFSADSFAAKGAVPLGGGLSHPGKFALIELQNGDPAVPGNEPVYVAGTE
                     NFYVITRYNWSSYYAMAVIELGREVMAVVGP"
     gene            complement(4263..>4499)
                     /locus_tag="PKV30_09755"
     CDS             complement(4263..>4499)
                     /locus_tag="PKV30_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30528.1"
                     /translation="VVIAKTIIDMAEDPQHELRLRFDDFVAEFITRLQHDPALTARAA
                     SWRKPASMRRRRRRRARCCKACTPAACRPSCASN"
BASE COUNT          795 a         1432 c         1507 g          765 t
ORIGIN      
        1 acggcgccgg caccgagcgg ctgattccgt tcgacctcat cccgcgcatc atccccgccg
       61 atgaatggga catgctggag cgcggcctga cgcagcgcgt gacggcgctc aaccgcttca
      121 tccacgacgt ttaccacgag caggagatat tgcgcgccgg catcgtgccg tccgaccagg
      181 tgctgcgcaa cgcccagtac cgcgccatca tgaaaggcgt ggcggtgccc aacgacgtgt
      241 attcgcacat cgccggcgtc gacatcgtgc gtgccggcac cgagggcggc aaaggcgagt
      301 actacgtgct ggaagacaac ctgcgcgtgc ccagcggcgt cagctacatg ctggaagacc
      361 gcaagatgat gatgcggctc ttccccggcc tgttcagcca gtaccgcgtg gcgccggtcg
      421 cgcactaccc cgatctgctg ctggaaaccc tgcgcgacag cagccccgcc gccaccgcca
      481 accccaccgt ggtggtgctg acgcccggca tgtacaacag cgcctacttc gagcacgcct
      541 ttctggcgca gcagatgggc gtcgagctgg tcgaggggca ggacttgttc gtcaaggacg
      601 acttcgtcta catgcgcacc acgcgcggcc ccaagcgggt ggacgtgatc taccgccggg
      661 tggacgacga cttcattgat tcgcaggtgt tccgccccga ttcggcgctg ggctgcgccg
      721 gcctgatgcg cgcctaccgc gccggccacg tgaccatctg caatgccgtg ggcacgggtg
      781 tggcggatga caagtcgatc tacccctacg tgcccaagat gatcgagttc tatctgggcg
      841 agaagccgat cctgaacaac gtgcccacct acatgtgccg cgacgccgac gacctgaagt
      901 acgtcatggc caacatgcag gacctggtgg tgaaggaagt gcacggcgcc ggcggctacg
      961 gcatgctggt cgggccggcg tccaccgcgg ccgaggtcga tgaattccgc aaggccgtgc
     1021 tggcgcggcc cgacggctac atcgcccagc ccacgctcag tctgtcgagc tgcccgacct
     1081 atgtggaaag cggcgtggcg ccgcgccaca tcgacctgcg gcccttcgtg ctgtcgggca
     1141 agacggtgca aatggtgccc ggcggcctga cgcgcgtggc gctcaagaaa ggctcgctgg
     1201 tcgtcaactc atcgcagggc ggcggcacca aggacacctg ggtgctggaa gaaagttcgg
     1261 cctcgggcgc ccgccgttcg gtggtggaac agattcaggg cccgacgtca gagcaggtcc
     1321 agcgttctgc gtttggcgtt cagcgccaat ccttctagcc cgcgccgtta gctttttcac
     1381 gctcaacgct caacgctcaa cgtggaacca aaaaatgctt tcccgtaccg ctgaccacct
     1441 cttctggatg tcccgctata ccgagcgggc cgagaacact gcgcgcatgc tcaatgtgca
     1501 ccacgaaacc tcgctgctgc cgcaatcggc ggccgtgtcg cagtacggct ggctcggcgt
     1561 gctctcgctc tcagagctgc tgccggccta caccgagcgc cagggcgagg tttcgccgcg
     1621 cggcgtgatg cgtttcatgg tgctggacga agaaaaccct tcgtccatcg tggcctgcct
     1681 gaaagccgcg cgcgaaaacg cccgcgccgt gcgcggcacg ctgacgaccg aggtgtggga
     1741 aacgctcaac cagacctggc tggagatgag ccgcctcgcg cgcagtggcg atttcgagga
     1801 agacccggcc gagttcttcg agtgggtcaa gttccactcg cacctctcgc gcggtgtcac
     1861 cctgggcacc atgctgcaag acgaagcctt tcacttcctg cgcttgggca cctttttaga
     1921 gcgcgccgac aacaccgcgc gcctgctcga tgtgaaattc cacgccgtag agagcgagtt
     1981 cttcggcacc gccagcgaga aggaccttga atacgacttc taccactggg ccgccatcct
     2041 gcgcagcgtc agcgccttcg agatctaccg caaggtgtac cgcgacgtca tcgtgcccga
     2101 gcgcgtggcc gagttgttga tgctgcgcga agacatgccg cgcgcgctgc accactgcat
     2161 gagcgaggtg ctgaccaacc tgcgcctggt cggcgccgaa ccgcagggag aaaccatgcg
     2221 ccacgccggc cgcctgcgcg ccgatctgcg ctatggccac atcgatgaaa tcctggccac
     2281 tgggctgcac gcgttcttga cgcagttcct ggatcgggtg aatcagctgg gcgggcacgt
     2341 cagtcgggat tttctggtgc cggcggggta aggagcgggg tggagaattc ggcctgctcg
     2401 ctgtcgtcct catttcgcca gggcgtgcat tgactttcat gattttgaca ttatcatgtg
     2461 catgatcatg tgcgggagaa atcaatgaaa tccgaacgca ttacctttct ggccaccccc
     2521 gaattcaagg ccaatctgac ccagttggcc gtgcgggaga acaccagcgt gggcgaggtg
     2581 atccgtgggc gtttcgagag ggctcagaat cgccctgacg cgtccgagga aagcgagttg
     2641 cgcgcgcttg tggccgattt gcgcgcggca accgccaagg cgcagaccag cttgagcgaa
     2701 gggctggccg aggctgaaca gactttgcgc gagctcagaa aatcgtccca gcgcaaggct
     2761 gccgcatgag cgcgctgacc gacgctgcca aggcgatcaa ggaagttctc aagctgaccg
     2821 acgatgtcaa gcgtgctggc gaggccctga aggacatctc gcgtgagctc cgtgaacatg
     2881 atcgtcgcat cacgcgactc gaagccaaat gggatacggc catgcagttg tcggcgacgc
     2941 ggcctttgcc gccgacagat cgcgcgtaat gtttgtgctg aagaggtgag ctggtagtcc
     3001 gaaggcgtcg aaattttgat ggggcagcgg ctcaaggccc gacgaccgcc atcacctcac
     3061 gccccaattc aatcaccgcc atcgcgtaat agctgctcca gttatagcgc gtgatgacgt
     3121 agaagttctc cgtaccggcg acgtagaccg gctcgttgcc cggcacggcg ggatccccgt
     3181 tctgcaactc gatcagcgcg aacttgcccg ggtgcgacag gccgccgcct agcggcacgg
     3241 cgcccttggc ggcaaagctg tcggcgctga aagacggcag gatgtcgggg gccaggagcg
     3301 cgtccatctg cgcttcgggc gccaggcgca cgccgtagtg cgtgggcacg ccgggctgcc
     3361 agccgtagcc cttgaagtag ttggcgaccg agccgatgac gtcggcttcg ctgttccaca
     3421 ggtcgatgcg gccgtcgccg tcgtagtcga tggcgtactt gacccagctg gacggcatga
     3481 actgcggcat gcccatggcg ccggcgtagc tgccgacggg ctgcagcggg tccatgccgg
     3541 tgcggtaaga cagcgataaa aactgctcca actcgtcctg aaaaaaagcc tgccgcgcct
     3601 gcgcgcgcgg gtgctggggc gggaagtcga aggtcagcgt ggccagcgcg tcgatgacgc
     3661 gaaagctgcc catgttctgg ccgtaaatcg tttcgacccc gacgatgccg acgatgattt
     3721 cgggcggcac gccgaattcg gcctgcgcgc gggcgagggt ggtgcggttg cgctcccaga
     3781 agcgggcgcc ggcgcgaatg cgcaccgggt cgatgaagcg gctgcggtac acgcgccagt
     3841 tcttggccgt gccgcgcgcg ggcggcagca tcaggcgcgg cacgttgggc aggtagacgg
     3901 cctggccgat ggcggagcgc acccagtcgg ccggcaggcc gcgccgcgcg gccaggtcgt
     3961 cggcaaagcg catggcctcg gcgcgctcgg cataggggat gtcgggcgcc cgttcggcac
     4021 gcggccttct tgaacgattt tgggcgccag cgcttgctgg taaagcgcca gcagctatgc
     4081 aaagcgtagc gagaacatcg cggcggttga atgcagtgaa atctgtcatg ggctgggtcg
     4141 cgggcgtggc caggccaagc gttgaaagtc gcgttgcagt gtagcgaggc tggggcctgt
     4201 gcgaacaccc tcgccgggcg cggcggcgta gcgcaggcgc tccagcgcga gcagccagtc
     4261 ggtcagttgc tggcgcaact cgggcggcag gctgctggcg tgcacgcctt gcagcagcgc
     4321 gcgcggcggc gcctgcggcg gcgcatcgac gccggcttgc gccagctggc ggcgcgcgcg
     4381 gtgagcgccg ggtcgtgctg caggcgagtg atgaactcgg ccacgaagtc gtcaaaacgc
     4441 aggcgcagct cgtgctgcgg gtcttcggcc atgtcgatga tggtcttggc gatcaccac
//
LOCUS       DAOMVO010000202         4486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5535_length_4486_cov_15.113970, whole genome shotgun sequence.
ACCESSION   DAOMVO010000202 DAOMVO010000000
VERSION     DAOMVO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_4486_cov_15.113970"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="PKV30_09760"
     CDS             <1..135
                     /locus_tag="PKV30_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30529.1"
                     /translation="NPPTGCTFHPRCPHANARCKAERPAMLHLHGIQVACHAVEEGRI
                     "
     gene            199..753
                     /locus_tag="PKV30_09765"
     CDS             199..753
                     /locus_tag="PKV30_09765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL30530.1"
                     /translation="MNDRRYLFLVASTREPGHVGNTEWLAQQAAAALPADVPQQWLHL
                     APMQLPPFVDRRHTTGTYAPPEGDLKILLDATLACTDLVLVAPVYWYSIPSSLKTYLD
                     HWSGWMRTPGVLFKDSMAPKRLHLITTSGDRAKAQPMIDSVRLCAEFLSMPWAGVLWG
                     KGGPPGAVAADAAALQAARDFLAA"
     gene            796..1743
                     /gene="tal"
                     /locus_tag="PKV30_09770"
     CDS             796..1743
                     /gene="tal"
                     /locus_tag="PKV30_09770"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRL30531.1"
                     /translation="MNQLDALKQFTTVVADTGDFKQLAAFQPQDATTNPSLILKAVQK
                     PEYAPLLTETVAAHRGQPLDEVVDRLLVRFGTEILSIIPGRVSTEVDARLSFDEDATY
                     TRAERLIELYQAAGVPTDRVLIKVAATWEGIRAAERLQRRGIHCNLTLLFSFCQAVAC
                     GQAKVQLISPFVGRIYDWYKKAAGAAWNEAAMAGAADPGVQSVKRIFDYYKRHGVATE
                     VMGASFRNVGQITALAGCDLLTISPELLAQLAASEAPLARALDAEAARKLDLPAVQYD
                     EAQFRLALNQDAMATEKLAEGIRSFIADAEKLDALMQAA"
     gene            1756..3360
                     /gene="pgi"
                     /locus_tag="PKV30_09775"
     CDS             1756..3360
                     /gene="pgi"
                     /locus_tag="PKV30_09775"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRL30532.1"
                     /translation="MDWQARPRCDEAAAWPLLQAHFAQHFAGTRAFDLRQAFAEDGAA
                     RAQRFSFEAAGVFADLSKNLIDAEAQRLLNQLACECGIEAQRDAMLAGERINTSEQRA
                     VTHMHWRYESNQAPALSGQARAASESIANTVANATAVHRAMLDFAEAVRADERITDVV
                     NIGIGGSDLGPRMVVQALRSSGHANGPRVHFIANMDGHELAELLPRLQADSTLFIIAS
                     KTFGTAETMQNAASARAWFLAQGGGDVARHFVALSANTAAVESFGAGCCFAFDEGVGG
                     RFSLWSSIGLSIAIAIGRAGFEALLAGAHAMDRHFASAPVENNLPVQLALLDLWNRNF
                     HHFASRCVAPYHHGLRSLPAYLQQLEMESNGKRVDTRGRALEAASAPVTWGQAGSNGQ
                     HAFFQMLHLGTDVVPVEFVLVRGAAHDLPDHHAKLLANALAQARALMVGQAGDAPERH
                     FPGNRPSTVLMLADLSPRSLGALLSMYEHRTFSLGALWGVNPFDQWGVELGKRHAGEI
                     EAALHAGDAVDLDASTAILIARLHGS"
     gene            complement(3380..4351)
                     /locus_tag="PKV30_09780"
     CDS             complement(3380..4351)
                     /locus_tag="PKV30_09780"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRL30533.1"
                     /translation="MILGARTAPASLSRRVLTRVALGGAAAIIAPASWAARKDSVAPR
                     YPLTPRQHFWLPHLSPSGPVVALVNLHTQMIQVFRDGLAIGFSSVSTGKAGYGTPSGV
                     YPVLQKARHHRSSTYNNAPMPYMLRLTWQGVALHGGHLPGYPASHGCIRLPHEFAPRV
                     FETVRLGDLVMVVRHALPSGASPLTLLAPITARAMPMLPAGALAAGNFWRAAPPAPGV
                     PLTILASLPQQRLYVLQAGALVAACDLPPMPPSVAPEGNGLCLWAVEGGWRAPGDATA
                     EALAAQLWPAGGELDLRLRPHLSAGSRLLITALPAISDMHRQLASVA"
BASE COUNT          771 a         1543 c         1458 g          714 t
ORIGIN      
        1 aacccgccca cgggctgcac ctttcacccg cgctgtccgc acgccaacgc gcgctgcaag
       61 gccgagcgac cggcgatgct gcacctccac ggcattcagg tcgcgtgcca cgcggtggaa
      121 gaaggccgca tttgacattt gctattgttt gagtagctgc tcgcgcttga tggatgagcg
      181 ccagcgcctg ttttgaccat gaacgaccgc cgctatctgt ttcttgtcgc ctccacgcgc
      241 gagcccggcc acgtcggcaa taccgaatgg ctggcgcaac aagccgccgc cgcattgccg
      301 gccgacgtgc cgcagcagtg gctgcacctg gcgccaatgc agctgccgcc ttttgtcgat
      361 cgccgccaca ccaccggcac ctacgcgccg cctgagggcg acttgaaaat cctgcttgac
      421 gccacgctgg cctgcaccga tctggtgctg gtcgcgccgg tttactggta cagcattccg
      481 tcctcgctca agacttacct cgaccattgg agcggatgga tgcgcacgcc cggcgtgctc
      541 ttcaaggaca gcatggcgcc caagcgcctg cacctcatca ccaccagcgg cgaccgcgcc
      601 aaggcgcagc ccatgatcga ctcggtgcgc ctgtgcgccg aatttctgtc gatgccgtgg
      661 gccggcgtgc tgtggggcaa gggcggcccg cccggcgcgg tggcggccga tgcggcggcg
      721 ctgcaggctg cgcgcgattt tctggccgcc tgagccggcc gcagagcgcc gccccgccgg
      781 cgggataatc gctgcatgaa ccagctcgac gcgctcaaac aattcaccac cgtcgtcgcc
      841 gacacgggcg acttcaagca actggccgcg tttcagccgc aagacgccac caccaacccc
      901 tcgctcatcc tgaaggcggt gcagaagccc gaatacgcgc cgctgctgac ggaaaccgtg
      961 gccgcgcacc gcggccagcc gctcgatgaa gtggtcgatc gcctgctggt gcgctttggc
     1021 actgaaatcc tctccatcat ccccggccgc gtgtcgaccg aagtcgatgc gcgcctgtcg
     1081 tttgacgaag acgccaccta cacgcgcgcc gagcgcctca tcgagctgta ccaggcggcc
     1141 ggcgtgccga cggatcgcgt gctgatcaag gtcgccgcca cgtgggaagg cattcgcgcc
     1201 gccgaacggc tgcagcggcg cggtatccat tgcaacctga cgttgctgtt cagcttctgc
     1261 caggccgtgg cctgcgggca ggccaaggtg caactgattt cgccttttgt cggccgcatc
     1321 tacgactggt acaagaaagc ggccggcgcc gcgtggaacg aagcggcgat ggcgggtgcg
     1381 gccgaccccg gcgtgcaatc ggtcaagcgc attttcgatt actacaagcg ccacggcgtc
     1441 gccaccgagg tgatgggcgc cagctttcgc aacgtcggcc agatcaccgc gctggctggc
     1501 tgcgacctgc tcaccatcag ccccgaactg ctggcgcaat tggccgccag cgaggcgccg
     1561 ttggcgcgcg cgctcgacgc cgaggcggcg cgaaaacttg atcttccggc cgtgcagtac
     1621 gacgaggcgc agtttcgtct cgccttgaat caggacgcca tggcgacgga gaagctggcc
     1681 gaaggcatcc gctcgttcat tgccgacgcc gagaagctcg acgccttgat gcaagccgcc
     1741 tgaggggtgt tctggatgga ttggcaagcc cgtccgcgct gcgacgaagc cgccgcctgg
     1801 ccgctgttgc aggcgcattt cgcgcagcac tttgctggga ctcgcgcttt cgatctgcgg
     1861 caagcgtttg ccgaagacgg cgcagcgcgc gcccagcgct tcagcttcga ggctgcgggc
     1921 gtgttcgcgg atctgtcaaa aaacctgatc gacgccgaag cgcagcgcct gctcaaccaa
     1981 ttggcgtgcg aatgcggcat cgaagcgcaa cgcgacgcca tgctggccgg cgagcgcatc
     2041 aacaccagcg agcagcgcgc cgtcacgcac atgcactggc gttatgaatc aaatcaggcg
     2101 ccagcgcttt caggacaagc gcgagcagct tctgaaagca tagcaaacac ggtggccaat
     2161 gccacggcgg tgcaccgcgc gatgctcgac ttcgccgaag ccgtgcgcgc cgacgaacgc
     2221 atcaccgacg tcgtcaacat cggcatcggc ggctccgatc tggggccgcg catggtggtt
     2281 caggcgctgc gctcgagcgg ccacgccaac ggcccgcgcg tgcatttcat cgccaacatg
     2341 gacggccacg agctggccga actgctgccg cgcctgcagg ccgacagcac gctgttcatc
     2401 atcgcctcca agacctttgg caccgccgag acgatgcaga acgccgcctc ggcacgagcg
     2461 tggtttttgg cgcagggcgg cggcgacgtg gcgcgccatt tcgtcgcctt gagcgccaac
     2521 accgccgccg tcgaatcgtt tggcgccggc tgctgctttg cgtttgacga aggcgtgggc
     2581 gggcgctttt cgctctggtc gagcatcggc ctgtcgattg ccatcgccat cggccgcgcc
     2641 ggttttgagg ctttgctggc cggcgctcac gccatggacc ggcactttgc cagcgcaccc
     2701 gtcgaaaaca acctgccggt gcaactggcg ctgctcgatt tgtggaatcg caacttccac
     2761 cactttgcca gccgctgcgt tgccccctac caccacggcc tgcgcagcct gccggcctat
     2821 ctgcaacaac tggagatgga aagcaacggc aagcgggtcg atacgcgcgg ccgtgccctt
     2881 gaggccgcca gcgcgcccgt cacctggggc caggccggca gcaacgggca acacgccttc
     2941 ttccagatgc tgcacctggg caccgacgtg gtgccggtcg aattcgtgct ggtgcgcggg
     3001 gctgcacacg atctgcctga tcaccacgcc aagctgctgg ccaacgcgct ggcgcaggcg
     3061 cgcgcgctga tggtcggaca ggcgggcgac gcgcccgagc gccacttccc cggaaaccgg
     3121 ccttccacgg tcttgatgct ggccgacctg tcgccgcgca gcctgggcgc cttgctctcc
     3181 atgtatgagc atcgaacctt ttcactgggt gcgctgtggg gcgtcaaccc attcgatcaa
     3241 tggggtgtgg aactgggcaa gcggcacgcg ggcgagatcg aagcggcgct gcacgcgggc
     3301 gatgccgtcg atctggacgc ctccaccgcg atcctgatcg cgcgcttgca cggcagctaa
     3361 gaaagccgcg cgccgaggct caagcgacgc tggccagctg gcgatgcatg tcgctgatcg
     3421 caggcaaggc ggtgatcaac agccggctgc ccgccgacag atgcggtcgc aggcgcagat
     3481 ccaactcgcc gcccgcaggc catagctgcg ccgccagagc ctcggctgtg gcgtcgcccg
     3541 gcgcacgcca gccgccttca accgcccaaa gacacagccc gttgccttcc ggcgccacgc
     3601 tcggcggcat gggcggcaag tcgcacgcgg cgaccagcgc gccggcttgc agcacataca
     3661 ggcgttgctg cggcaagctg gccagtatcg tcagcggcac gcccggcgcc ggtggcgccg
     3721 cgcgccagaa gttgcccgcc gccagcgcgc ccgccggcag catgggcatc gcccgcgccg
     3781 tgatcggcgc cagcagcgtg aggggcgagg cgccgctcgg cagcgcatgg cgcaccacca
     3841 tcaccagatc gcccaggcgc acggtctcga acacacgcgg cgcaaattcg tgcggcagcc
     3901 ggatgcagcc gtgcgacgcc ggatagcccg gcaaatgccc gccgtgcagc gccacgccct
     3961 gccacgtcag ccgcagcatg taaggcatgg gcgcgttgtt gtaggtgctg gatcggtggt
     4021 gccgcgcctt ttgcagcacc gggtacacgc cggacggcgt gccataaccc gccttgcccg
     4081 tgctgacgct tgaaaagcca atcgccaaac cgtcgcgaaa cacctggatc atctgcgtgt
     4141 gcaggttgac cagcgccacc accgggccgc tgggcgacag gtgcggcagc cagaaatgct
     4201 gacgtggcgt cagggggtag cgaggggcga cagagtcttt gcgagcggcc caactggctg
     4261 gcgcgatgat cgccgctgcg ccgcccaggg caacccgcgt cagcacgcga cgtgagagcg
     4321 aggcaggggc cgtgcgtgcg ccaaggatca tgaaaacgag caagaacgtt gaggtgacaa
     4381 atcgtagcaa atcacctcgg acaagatgca ctgatggtgc gattgactac gatatcgagc
     4441 gccggcgctt gataggtaag cgcgagcagc tatcgttttt gatggc
//
LOCUS       DAOMVO010000203         4479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5550_length_4479_cov_16.045434, whole genome shotgun sequence.
ACCESSION   DAOMVO010000203 DAOMVO010000000
VERSION     DAOMVO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_4479_cov_16.045434"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKV30_09785"
     CDS             <1..336
                     /locus_tag="PKV30_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRL30534.1"
                     /translation="SWQTVLLLMASNVFMTFAWYGHLKNLATAPWYIAALISWGIALF
                     EYLLQVPANRIGHTQFSVGQLKIMQEVITLVVFVPFAVLYLREPLKLDYLWAALCMVG
                     AVYFIFRSA"
     gene            444..1091
                     /locus_tag="PKV30_09790"
     CDS             444..1091
                     /locus_tag="PKV30_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HRL30535.1"
                     /translation="MLFSKLLPKEGNFFELFNQHADRVVEAAQAFQQLVAHYADVHLR
                     AKYAQDVDNAERAADRITHEVNRLIHTTFITPIDREQIHGLINLMDDVADLLQDSAET
                     MALYDVHHMTDEIVRLTDLSLKCCQRVQAAVRLLGKIAEPATAEAALKTCEEIDQLES
                     DADRVMRSAMSKLFREEPDVRELIKMKAIYELLETVTDKCEDVANQIEGIVLENS"
     gene            1216..2226
                     /locus_tag="PKV30_09795"
     CDS             1216..2226
                     /locus_tag="PKV30_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRL30536.1"
                     /translation="METAQAALWVVVMLVALAIAFDFMNGFHDAANSIATVVSTGVLK
                     PTQAVVFAAFFNFIAIFVFHLSVAATVGKGIVQPGVVDVHVVFGALIGAITWNLVTWY
                     YGIPSSSSHALIGGIVGAVIAKGGIGQLIAGGILKTVAFIFISPLLGFLFGSLMMVAV
                     AWIFRRTRPSRIDKWFRRLQLVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYTAAGDS
                     APPLWAIISCYTAIAAGTMFGGWRIVKTMGQKITKLKPVGGFCAETGGALTLFIATGL
                     GVPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWVLTIPASAFVAALAYWISWG
                     LF"
     gene            complement(2296..2748)
                     /locus_tag="PKV30_09800"
     CDS             complement(2296..2748)
                     /locus_tag="PKV30_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30537.1"
                     /translation="MRNKNKTLATWLTFLGGPLGLHRFYLKGFGDLLGWLLPIPTALG
                     LYGIERVQQLGQDDHWSWLLIPLLGFTIAGCALMAIIYGLMTPEKWNARFNPQLDPDA
                     APGRTNWLTVFGIAAALMIGTAVLMASLAFSFQRYFEYQIEEARKISQ"
     gene            2961..3221
                     /gene="rpsP"
                     /locus_tag="PKV30_09805"
     CDS             2961..3221
                     /gene="rpsP"
                     /locus_tag="PKV30_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRL30538.1"
                     /translation="MVVIRLSRGGSKGRPFFNIVVADKRVRRDGRFIERIGFYNPGAV
                     EGEEGLRVATDRLNHWIGVGAQASPTVQRLVRQAAKAQPATA"
     gene            3224..3796
                     /gene="rimM"
                     /locus_tag="PKV30_09810"
     CDS             3224..3796
                     /gene="rimM"
                     /locus_tag="PKV30_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518084.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRL30539.1"
                     /translation="MIPPTAPEPAELPADAVEVGRVLDAWGIKGWLKIQPYSASPEAL
                     FSSKRWYLQPPERVVKPAFTGTLLLKIKEAREHGASVVASAQEVPDRNAAEALKGARI
                     FIARSSFPTPEEGEYYWVDLIGLAVVNRERVALGQVTDLMATGPQQVLVIGYEADGKA
                     QERMIPFVDAYVDEVDLQAKRITVDWQPDY"
     gene            complement(3812..4090)
                     /locus_tag="PKV30_09815"
     CDS             complement(3812..4090)
                     /locus_tag="PKV30_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRL30540.1"
                     /translation="MTNRVVPFVLIVLLVVIHAQLWVGRGSLPNVTALQRKVDQQKAA
                     NAQARLVNEQLASEVQDLRAGLGMVEERARIELGMVKPNEIFVQVTPR"
     gene            complement(4165..>4479)
                     /gene="eno"
                     /locus_tag="PKV30_09820"
     CDS             complement(4165..>4479)
                     /gene="eno"
                     /locus_tag="PKV30_09820"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRL30541.1"
                     /translation="EGIDKHIANSILIKINQIGTLTETFQAIEMAKRAGYTAVISHRS
                     GETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKYNQLLRIEEDLGDMAEYPGRAAFYN
                     LR"
BASE COUNT          789 a         1395 c         1468 g          827 t
ORIGIN      
        1 tcgtggcaga ccgtgctgct gctgatggcc agcaacgtct tcatgacgtt tgcgtggtac
       61 gggcacctga agaatctggc caccgcgccg tggtacatcg cggcgctgat cagctggggc
      121 attgcgctgt tcgagtacct gctgcaggtg ccggccaacc gcatcgggca cacgcagttc
      181 agcgtcggtc aactcaagat catgcaggag gtgatcacgc tggtggtgtt tgtgccgttt
      241 gccgtgctgt acctgcgcga gcccctcaag ctcgactacc tttgggcggc gctgtgcatg
      301 gtgggcgcgg tgtatttcat cttccgcagc gcttgaggct tgtcagccag ccggcgcgtt
      361 ggcgcggcta cacttgagaa aagtcatgaa acggtcatgc cgatttcacg cactcgtcat
      421 agaaacccgc ggagccccca cccatgctgt tcagcaagct gttgcccaag gagggcaatt
      481 tttttgagct gttcaaccag cacgccgacc gcgtcgtcga ggcggcgcag gcgtttcagc
      541 agctggtggc gcactacgcc gacgtgcatt tgcgcgccaa gtacgcgcag gatgtggaca
      601 acgccgagcg cgccgccgac cgcatcacgc acgaggtcaa ccgcctgatt cacaccacct
      661 tcatcacgcc gattgaccgc gagcagattc acggcctgat caacctgatg gacgacgtgg
      721 ccgatctgtt gcaggactcg gccgagacca tggcgctgta cgacgtccat cacatgaccg
      781 atgaaatcgt gcgcctaacc gatctgtcgc tgaagtgctg ccagcgcgtg caggcggcgg
      841 tgcggctgct cggcaagatc gccgagccgg ccaccgccga ggcggcgctc aagacctgcg
      901 aggagatcga ccagcttgaa tccgacgccg accgcgtgat gcgcagcgcc atgagcaagc
      961 tgttccgcga agagcccgac gtgcgcgagc tgatcaagat gaaagccatt tacgagctgc
     1021 tggaaaccgt caccgacaag tgcgaggacg tggccaacca gatcgaaggc atcgtgctgg
     1081 aaaactcatg acgaaatgac cttgctgctg cgcttcgatg acggcgcctg agcgccatga
     1141 cgggcccgtc gtttcgtcat tgaggccgca acgcggcgaa gtcattctgt tattggcaaa
     1201 cccccgaaaa catccatgga aactgctcaa gccgccctct gggtcgttgt gatgctggtc
     1261 gcgctggcga tcgcgttcga cttcatgaac ggctttcatg acgccgccaa ctcgatcgcc
     1321 accgtggttt ccaccggcgt gctgaaaccc acgcaggcgg tggtgtttgc ggccttcttc
     1381 aacttcatcg ccatcttcgt gtttcacctc agcgtggcgg cgacggtggg caagggcatc
     1441 gtgcaaccgg gcgtggtgga cgtgcatgtg gtgtttggcg cgctgatcgg cgccatcacc
     1501 tggaatttgg tgacctggta ctacggcatt cccagcagct cgtcgcacgc gctgatcggc
     1561 ggcatcgtgg gtgcggtgat cgccaagggc gggatcgggc agttgatcgc cggcggcatt
     1621 ctgaagacgg tggccttcat cttcatctcg ccgctgctgg gctttctgtt cggctcgctg
     1681 atgatggtgg cggtggcgtg gatcttcagg cgcacgcgcc cgagccgcat cgacaaatgg
     1741 tttcgccggc tgcaactggt gagcgccggc gcctacagcc tgggccacgg cggcaacgac
     1801 gcgcaaaaga ccatcggcat catctggatg ctgctcatcg ccaccggcta cacggccgcg
     1861 ggcgacagcg cgccgccgct gtgggccatc atcagctgct acacggcgat tgcggcgggc
     1921 accatgtttg gcggctggcg catcgtcaag accatggggc agaagatcac caagctgaaa
     1981 cccgtgggcg gcttctgcgc cgagacgggc ggggcgctga cgctgttcat cgccaccggg
     2041 ctgggcgtgc cggtgtcgac cacgcacacc atcaccggcg ccatcgtcgg cgtgggctcc
     2101 acccagcgcg cgtcggcggt gcgctggggc gtggcgggca acatagtgtg ggcgtgggtg
     2161 ctgaccatac cggccagcgc gttcgttgcg gcgctggcgt actggatcag ctgggggttg
     2221 ttctaaaaac catcaaaaat gtagctgtct gcgctttctg ggcaagcgct ggaggcattt
     2281 ttttgatcag agcccctatt gcgagatctt gcgcgcctct tcgatctggt attcgaagta
     2341 acgctgaaag ctgaacgcca gcgacgccat gagcaccgcc gtgcctatca tcagcgcggc
     2401 ggcgatgccg aagacggtca accagttcgt gcggccgggc gccgcgtcag ggtcaagctg
     2461 cgggttgaag cgcgcgttcc atttctcggg cgtcatcagg ccgtagatga tggccatcag
     2521 cgcgcagccg gcgatcgtga agccaagcag cggaatcagc agccagctcc agtggtcgtc
     2581 ctgccccaac tgctgcacgc gctcgatgcc gtacaggccc agcgcggttg gaatcggcag
     2641 cagccagccc agcaggtcgc caaagccctt gaggtaaaag cggtgcaggc cgagcggccc
     2701 gccgaggaaa gtgagccagg tggcaagggt cttgttcttg ttgcgcatgc ggagaggggt
     2761 gagcagggga aaagagggcg cacgtacggc cgggcggcgt gtgctgagag aggccagcta
     2821 taatcatagg cttttcgatg tgtcgctggc cgggtggcca tgtcgcgtgt ctcaacatcg
     2881 gaagaacctg ggcgccttga agctggggcc acaacccgca gccagggcgc cgagtccacc
     2941 acccagagat tgcacacatc atggtcgtta ttcgtctttc ccgcggcggc tccaagggcc
     3001 gtcctttctt caacatcgtc gtcgccgaca agcgcgtgcg ccgggatggt cgcttcatcg
     3061 agcgcatcgg cttctacaac cccggcgcgg tcgagggtga agaaggcttg cgcgtcgcca
     3121 ccgaccgcct gaaccactgg atcggcgtcg gcgcccaggc gtcgcccacg gtgcagcgcc
     3181 tggttcgcca ggccgccaag gcccagcccg ccaccgcctg agcttgatcc cgcccacagc
     3241 gcccgagccg gccgagctgc cggcagacgc ggtcgaagtc ggccgcgtgc tggacgcctg
     3301 gggcatcaag ggctggctca agattcagcc ctacagcgcc tcgcccgagg cgcttttttc
     3361 ttccaaacgc tggtatctgc agccgcccga gcgcgtggtg aagcccgcct tcaccggcac
     3421 gctgctgctg aagatcaagg aagcgcgcga acacggcgcc agcgtggtcg ccagcgcgca
     3481 agaagtcccc gaccgcaacg ccgccgaagc gctgaaaggc gcgcgcatct tcatcgcccg
     3541 atcgtccttc ccgacgccgg aagagggtga gtactactgg gtcgacctga tcggcctggc
     3601 cgtggtcaac cgagaacgcg tggcactggg ccaggtgact gacctgatgg cgaccggccc
     3661 gcagcaggtg ctggtgatcg gctacgaagc agacggcaag gctcaggagc ggatgattcc
     3721 gtttgtggac gcctatgtgg acgaggtcga tctgcaggcc aagcggatca cggtggactg
     3781 gcagccggat tactgagccc ggcgcgcgtt gtcagcgcgg cgtcacctgc acaaaaatct
     3841 cattgggctt gaccatgccc agctcgatcc gcgcgcgctc ttcgaccatg ccgaggcccg
     3901 cgcgcaaatc ctgcacttca gacgccagtt gctcgttgac caaccgcgcc tgggcgttgg
     3961 cggccttttg ctgatccacc ttgcgctgca gcgccgtcac gttgggcagg ctgccgcggc
     4021 cgacccacag ctgggcgtgg atgacgacca gcagcacgat cagcacgaac gggacgacgc
     4081 ggttggtcat gacatgggtg aaaggccagc ggcgtgggac tgggctgcta tcgtttcagt
     4141 atagcgccag accctgacag cgccttacct caggttgtag aaggccgcgc gccccgggta
     4201 ttcggccatg tcgcccaggt cttcctcgat gcgcagcagc tggttgtact tggccatgcg
     4261 gtcggagcgc gacagcgaac cggtcttgat ctgccccgcg ttggtgccca cggcaatgtc
     4321 ggcaatcgtg gaatcttcgg tctcgcccga gcggtggctg atgaccgcgg tgtagcccgc
     4381 gcgcttggcc atctcgatgg cctggaaggt ctcggtcagc gtgccgatct ggttgatctt
     4441 gatgaggatc gagttggcga tgtgcttgtc gatgccttc
//
LOCUS       DAOMVO010000204         4441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5622_length_4441_cov_11.735294, whole genome shotgun sequence.
ACCESSION   DAOMVO010000204 DAOMVO010000000
VERSION     DAOMVO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5622_length_4441_cov_11.735294"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1255
                     /locus_tag="PKV30_09825"
     CDS             71..1255
                     /locus_tag="PKV30_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30542.1"
                     /translation="MNSRELLRLVTAQVCVHSAMTGARLAAPLLALQLGYSAAQVGVL
                     LALFALSPVVLALPAGRLADRHGLRLPLRLAIGAAMLGTALAAALPTFGVLCVMALLT
                     GASAGTAQIALQRHVGRAVAHPSELKTVFSWIAIAPAMANFLGPLVTGLLIDHAGPQP
                     AHETGFRVAFGVLALLPLLCWALVRGAPEQPLPPPAPQEAGKSVWDLIGVPMLRRLLF
                     VNWLQAMAWDVHTFVLPILGHERGMSASTIGALLGAFAIAAAGVRMALPLIAEKLPEW
                     KVMYGATLLAACALMVYPLMPGALTMGVCSVVLGVALGAVQPMVLSLLHQVAPPQRQG
                     EAMALRVMTINFSSFLMPMLFGSIGAVTGVAGLFWLVCGALALGARAIPALQVAEPPD
                     TP"
     assembly_gap    1526..1625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1736..2191)
                     /locus_tag="PKV30_09830"
     CDS             complement(1736..2191)
                     /locus_tag="PKV30_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL30543.1"
                     /translation="MTPQEKLQQLGITLPPVSAPAAAYVPFVRTGNLVFLSGHIARKD
                     GCAWVGKLGDTLDTAEGKLAARAIAIDLMGTLQAAVGDLNNIKRIVKVMSLVNSTPHY
                     TEQHLVTNGCSELLGEVFGDKGQHARSAFGVAQIPLGACVEIELVAEVA"
     gene            complement(2221..3153)
                     /locus_tag="PKV30_09835"
     CDS             complement(2221..3153)
                     /locus_tag="PKV30_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRL30544.1"
                     /translation="MRLRITHDTRYEYEPAVQTALHVAHLVPPPTRCQERAESRLEVD
                     PTPATLTETLDIYRNVRAFFEIDAAHDHLHVRATSLVNTWAPEPVDSQLGWDAVQDAF
                     VYRRGAQWHPAAEFIYPSIHVHPGALFADYARPSFAPGRPLIDAARELMQRVHAEFTY
                     RADSTQINTPAAVALERRMGVCQDFSHVMLACLRSLGLPARYVSGYLLTQPPPGMPRL
                     VGSDASHAWVSVFLPDVAARTGGHGWYDLDPTNCRDGWGSPGEDFIRLAVGRDYADIS
                     PMRGVLHGSGKHKLDVGVTVEPFEAASLADAPPV"
     gene            complement(3330..>4441)
                     /locus_tag="PKV30_09840"
     CDS             complement(3330..>4441)
                     /locus_tag="PKV30_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRL30545.1"
                     /translation="GGSSADTWVLGDEPLQEDTDFDHSTLAAFTPADTELVQRERLVT
                     SRAAENLFWLGRYAERAENATRLARIVLQSLHGEEEPSIGHLMWIGQLAESAGLVPDD
                     CPSPLDDRVEFERQLIGALGDTEDTPSVGYTLRNLRQAGSALRERLSPEHWNFIKQAE
                     DNFLREARRLRDDGGQGADALQQLAAVSRALSAITGAQHDRMWRDDGWRLLSAGRQIE
                     RLTYLSEALSRGFYTNAVHDAAGFGVLLDLFDSTISFHARYQRSRAIAALIEHVVLNF
                     ENPRSLGWVVQTLNGRLARLHEREPGPLEDLSQRLQPPGADELTRLCQSDHIGDFVHL
                     QALLQQFMKAGAQLSTDISLRHFTHTGEVRRSTGV"
BASE COUNT          620 a         1389 c         1579 g          753 t
ORIGIN      
        1 gcgccccgaa aattcctgaa ttgatagctg ctcgcgcttg ccagacaagc gctggcgccc
       61 gatttgaccc atgaacagcc gcgaactgct gcgcctggtc accgcgcagg tgtgcgtgca
      121 cagcgccatg accggtgcgc ggctggcggc cccgctgctg gctttgcagc tgggctacag
      181 cgcggcgcag gtgggcgtgc tgctggcgct gtttgcgctc tcgccggtgg tgctggcgtt
      241 gccggcgggg cggctggccg accggcacgg cttgcgcctg ccgctgcggc tggccatcgg
      301 cgcggccatg ctcggcacgg cgctggcggc ggcgttgccg acgtttggcg tgctgtgcgt
      361 gatggcgctg ctcaccggcg cgtcggccgg cacggcgcag attgcgctgc agcgccacgt
      421 gggccgcgcg gtggcgcacc cgtcggagct gaaaacggtg ttcagctgga tcgccattgc
      481 gccggccatg gcgaattttc tcgggccgct tgtcaccggg ctgctgatcg accacgcggg
      541 gccgcaaccg gcgcacgaga ccggttttcg cgtcgccttt ggcgtgctgg ccttgttgcc
      601 gctgttgtgc tgggcgctgg tgcgcggcgc gcccgagcaa ccgctgccac cgccggcgcc
      661 gcaggaagcc ggcaagtcgg tgtgggacct gatcggcgtg ccgatgctgc ggcgcctgct
      721 gttcgtcaac tggctgcagg ccatggcgtg ggacgtgcac accttcgtgc tgccgatcct
      781 ggggcacgag cgcggcatgt cggcgtcgac catcggcgcg ctgctgggcg cgtttgccat
      841 tgccgccgcg ggggtgcgca tggcgctgcc gctgatcgcc gagaagctgc ctgaatggaa
      901 agtgatgtac ggcgccacgc tgctggccgc ttgcgcgctg atggtctacc cgctgatgcc
      961 gggcgcgctg acgatgggcg tgtgctcggt ggtgctgggt gtggcgctgg gcgcggtgca
     1021 gcccatggtg ctgagcctgc tgcaccaggt ggcgccgccg cagcgccagg gcgaggcgat
     1081 ggcgctgcgc gtgatgacca tcaacttctc cagctttctg atgcccatgc tgtttggctc
     1141 catcggcgcg gtgaccggcg ttgccgggct gttctggctg gtgtgcggcg cgctggcgtt
     1201 gggggcgcgg gcgattccgg cgctgcaggt cgctgagccg ccagacaccc catagccgga
     1261 ctcaagaacg cagagatttc gcagaggacg cagaaaattc aaagaaagct ttttctggct
     1321 gttctttcgc gtccttcgcg tatggttttt ggttttcaag ccaaaacagg ctccggcgct
     1381 tgtccatcaa gcgcgggcag ctaccaaata gagagcgttg agctgacatc accactcatt
     1441 cgccagccca tcatccgttc gggctgagcc attcggcaag ctcatgacag gcttgtcgaa
     1501 tcctgtttct ctggcaccca gtcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnattcg gcaagctcat gacaggcttg tcgaatcctg tttctctggc acccagtcct
     1681 tctacaggct caggacgaac ggtgtgggat cattttttgg ttttgtctac cagcgtcagg
     1741 ccacttctgc gaccagctcg atctcgacgc acgcgccaag cggaatctgc gccacgccaa
     1801 acgcgctgcg ggcatgctgg cccttatcgc cgaacacctc gcccagcagc tcggagcagc
     1861 cgttggtgac caggtgctgc tcggtgtagt ggggcgtgga gttgaccagg ctcatcacct
     1921 tgacgatgcg cttgatgttg ttcaagtcgc ccacggcggc ctgcagcgtg cccatcaggt
     1981 cgatggcgat ggcgcgcgcg gccagcttgc cttcggccgt gtccagcgtg tcgcccagct
     2041 tgccgaccca ggcacagccg tccttgcgtg cgatgtggcc ggacaagaac accaggttgc
     2101 cggtgcgcac gaagggcacg taggcggcgg cgggcgcgct gacgggcggc agggtgatgc
     2161 cgagttgctg cagtttttct tgcggggtca tgaggatctc ctgggttgga aaaaaagtgg
     2221 ctacacgggc ggcgcgtcgg ccagcgaggc ggcctcgaat ggctcgaccg tgacgccgac
     2281 atcgagcttg tgcttgcccg agccatgcag cacgccgcgc attggcgaga tgtcggcgta
     2341 gtcgcggccc acggccagac gaatgaagtc ttcgccgggc gagccccagc cgtcgcggca
     2401 gttggtcggg tcgaggtcgt accagccgtg gccgccggtg cgggcggcca cgtcgggcaa
     2461 gaaaaccgac acccaggcgt gcgacgcatc gctgccgacc aggcgcggca tgccgggcgg
     2521 cggctgcgtc agcaggtagc ccgagacata gcgcgccggc agcccgagcg agcgcaggca
     2581 ggccagcatg acgtggctga agtcctggca cacgcccatg cggcgttcca gcgccacggc
     2641 ggcgggggtg ttgatctggg tgctgtcggc gcggtaggta aattcggcgt gcacgcgctg
     2701 catcagctcg cgcgcggcgt cgatcagcgg ccgcccgggc gcaaacgagg gccgtgcgta
     2761 gtcggcaaac agcgcgccgg gatggacgtg gatcgagggg tagatgaatt cggccgccgg
     2821 gtgccattgg gcgccgcggc ggtagacgaa ggcgtcctgc accgcgtccc agccgagctg
     2881 gctgtcgacc ggctcgggcg cccaggtgtt gaccaggctg gtggcgcgca cgtgcagatg
     2941 gtcgtgcgcg gcatcgattt cgaagaaagc gcgcacgttg cggtagatgt cgagcgtctc
     3001 ggtcagcgtg gcgggcgtgg ggtcgacttc cagccgcgac tcggcgcgct cctggcagcg
     3061 cgtgggcggc ggcaccaggt gcgccacgtg cagcgcggtt tgcaccgccg gctcgtactc
     3121 gtagcgcgtg tcgtgggtga tgcgcagccg catgtcagtg gaccgaggca ttgcgcgacg
     3181 ctggccgcgc atgaagcaca aagagcccga tgggcgcgga gcaggccatg cgcgggcacc
     3241 catggccggg gtttccctgg acattgggtg cgtccccctc ccgcaggaga gggggaaggc
     3301 gccgcaggcg acacaggggg tgtctcactt cacacccccg tcgagcgccg cacttcgccc
     3361 gtgtgcgtga agtgccgcag gctgatgtcg gtcgacaact gcgcgccagc tttcatgaac
     3421 tgctgcaaca gcgcctgcag gtggacgaag tcgccgatgt ggtccgactg gcacaggcgt
     3481 gtgagttcat ccgcgccggg cggctgcagg cgctgcgaca ggtcttccag cgggccgggt
     3541 tcgcgctcgt gcaggcgcgc caggcgcccg ttcagcgttt gcaccaccca gccgagcgag
     3601 cgcgggttct cgaagttgag caccacgtgc tcgatcagcg cggcgatcgc gcggctgcgc
     3661 tggtagcggg cgtggaagga gatggtggag tcgaacaggt cgagcaacac gccgaaaccc
     3721 gccgcgtcgt gcacggcgtt ggtgtagaag ccgcgcgaca aggcttccga caggtaggtc
     3781 agccgctcga tctggcgccc ggcgctcagc aggcgccagc cgtcgtcgcg ccacatgcgg
     3841 tcgtgctgcg cgccggtgat ggccgacagc gcgcgcgaca ccgccgccaa ctgctgcagc
     3901 gcatcggcgc cctggccgcc atcgtcgcgc aggcggcgcg cctcgcgcag gaagttgtcc
     3961 tcggcttgct tgatgaagtt ccagtgctcg ggcgacagcc gctcgcgcag ggccgagccg
     4021 gcctggcgca ggttgcgcag tgtgtagccg acgctgggcg tgtcctcggt gtcgcccagc
     4081 gcgccgatca gttgccgctc gaactccacc cggtcgtcga gcggtgacgg gcagtcatcg
     4141 ggcaccaggc cggccgattc ggccagttgg ccgatccaca tcaggtggcc gatggagggc
     4201 tcctcctcgc catgcagcga ctgcagcacg atgcgcgcca ggcgggtggc gttttcagcg
     4261 cgctcggcgt agcgcccgag ccagaacagg ttctcggccg cgcggctggt gaccaggcgt
     4321 tcgcgctgca ccagttcggt gtcggccggc gtgaaggcgg ccagcgtgga atggtcgaaa
     4381 tcggtgtctt cttgcagcgg ctcgtcgccc agcacccagg tgtcggcgct gctgccgccc
     4441 a
//
LOCUS       DAOMVO010000205         4433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5629_length_4433_cov_14.820923, whole genome shotgun sequence.
ACCESSION   DAOMVO010000205 DAOMVO010000000
VERSION     DAOMVO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_4433_cov_14.820923"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..714
                     /locus_tag="PKV30_09845"
     CDS             226..714
                     /locus_tag="PKV30_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRL30546.1"
                     /translation="MEAWLQQLLAALALPEYGLSTVFVVSFVSATLLPLGSEPAVFGL
                     IKLNPHLFWPTILVATAGNTLGGVVTWWMGYGAHQMVDKYRDSPTHLRALDWLRRIGP
                     PACFLSFLPIIGDPLCAVAGWLKMPLAPCTAWMAVGKFARYVVMTAALLAAWPGVLRF
                     AT"
     gene            783..1466
                     /gene="alkB"
                     /locus_tag="PKV30_09850"
     CDS             783..1466
                     /gene="alkB"
                     /locus_tag="PKV30_09850"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HRL30547.1"
                     /translation="MTVDLFADHTPPPGEVVMLGPSARVLRGFALPRMDALWAALQDV
                     TLQAPFRHMVTPGGQRMSVALTNCGALGWVSDRRGYRYTPLDPDNGAPWPALPVEFLS
                     LAHDAAAAAGFAGFVPDACLINRYQPGARMSLHQDRDERDLSHPIVSVSLGVPAVFLW
                     GGLRRADRPARVLLFHGDVVVWGGVDRLRFHGVAPVRLSTHALTGGERINFTLRRAGN
                     APDPMVSSN"
     assembly_gap    1564..1663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1664..2085
                     /locus_tag="PKV30_09855"
     CDS             <1664..2085
                     /locus_tag="PKV30_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRL30548.1"
                     /translation="AAVNVPLLLLLGAPVAAVLAYTLLANVVSVFSHANVGLPMLEHA
                     LRHILVTPAFHRVHHAADRPWTDRNFATVFPIFDRLLGTAATLPAQVQQSITLGLEHF
                     RSAPKQQLGGLLAQPFVAPGERSQPPRKRAPTADVST"
     gene            complement(2078..2284)
                     /locus_tag="PKV30_09860"
     CDS             complement(2078..2284)
                     /locus_tag="PKV30_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30549.1"
                     /translation="MKLWPLWIGLAFIGIYAALYYGGWVNAAAAFVVALVLAPLLYVL
                     VVMLFFDDTRRKPPPDRPPESGVK"
     gene            complement(2340..>4433)
                     /locus_tag="PKV30_09865"
     CDS             complement(2340..>4433)
                     /locus_tag="PKV30_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30550.1"
                     /translation="YPGYMMGERMEGQRAAERLDQVTKGLDPELAAQVVDKSLPKLEQ
                     YAKVYQDTYKTPVIGEKEMGTTLKVLERSLDTPTGKANMDRMLKMGMWNAPAVTQHIA
                     EGGRPDYAIAFGQQPKKEGLYGDYAAISKHVIETGTTQFREKVTEDARAYAGHMEEIG
                     WLIQNQGGTMTPDQLKKAIADYTTNKLKDDPNWEADGKRMQQTLADDGKKLLAQMASL
                     GQGANNGATSDASVEALKKTLDDPASRAAVSTALQADPNLASGPMGQRVLDFLVNPNL
                     SGPAKLANRSVWLGQEFANAYVKSNVLGRLEGLDRNDPASIQKAKDALASMRDSRFAK
                     LMGVSPEQMNKAVDAIENGALPKLGDDTQSLVKRLHTVDQELGDIKGFEKSTVPGQML
                     RAVGVGLAGVGLMASGAKAIDDPTLANNLRVLSDAAGLVWRGAEIGVGTGVLKAESKL
                     GWLGGSTFSKITGVMGATVDAIAAYDAFSKGDNVSGGLYVAQGTGMMMATFGASAASG
                     LEAAGVAGAAALGGPIVWVGVGIMAVAAVGMWAWNDHKANSQHEPDFDKGRSLAFLKQ
                     AGIADDAARTLCDQSGGGYSAVPLLVKYAQTQGLNLQNGADQAKFQDWINHMDPAKLT
                     QLKDRLHATATRLEGDVGKFGATAGDDASYQPSVTRYYPYPYSSPTGNVTSHPAPVSA
                     VQLEKVLGELGVPAL"
BASE COUNT          635 a         1517 c         1380 g          801 t
ORIGIN      
        1 agccgcggca cagctggcgg aacggccgat ccgtcacccc cgccatgggg gcgacaaaca
       61 agcggttggg caggctgtgg ggaccgatct gcatggtgaa aaagcggcgc ggccaaggcg
      121 gcacgtgaag gccggaaggc agcgggggag cggattctac ctgcccaaaa tttcagcagt
      181 cgaaaccacg cgtcgccccg tggcgcgcgc ctacactcgg gcgcgatgga agcctggctg
      241 caacaactcc tcgccgcgct ggcgttgcct gaatacggcc tgtcgacggt gttcgtggtc
      301 tccttcgtct cggccacgct gctgccattg ggctctgagc cggcggtgtt tggcttgatc
      361 aagctcaacc cgcacctttt ctggccgacc attctggtgg ccacggccgg caacacgctg
      421 ggcggcgtgg tgacgtggtg gatgggctac ggcgcgcacc agatggtcga caagtatcgc
      481 gattcgccca cccacctgcg cgcgctcgac tggctgcgcc gcatcgggcc gccggcgtgc
      541 tttctgtcct ttctgccgat catcggcgac ccgctgtgcg ccgtcgccgg ctggttgaag
      601 atgccgctgg caccctgcac cgcgtggatg gcggtcggca agttcgcgcg ctacgtggtg
      661 atgacggcgg cgctgctggc cgcgtggccc ggcgtgctgc gctttgctac gtgattgata
      721 gctgcttgcg cttgccagtc aagcgctggc agcatttttt tctcagcttt gaccgacacc
      781 cgatgaccgt cgacctgttt gccgatcaca cgccgccgcc cggcgaagtg gtgatgctcg
      841 ggccatcggc ccgcgtgctg cgcggctttg cgctgccgcg catggatgcg ctttgggcgg
      901 cgctgcaaga cgtcaccctg caagcgccgt ttcgccacat ggtcacgccc ggcgggcagc
      961 gcatgtcggt ggcgctgacc aactgcggcg cgctcggctg ggtgagcgac cggcgcggct
     1021 accgctacac accgctcgac cccgacaacg gcgcgccctg gcccgcgttg cccgtcgagt
     1081 tcttgagcct ggcgcacgat gccgccgccg cagccgggtt tgccggcttt gtgcccgacg
     1141 cctgcctgat caaccgctac cagcccggcg cccgcatgtc gctgcaccag gaccgcgacg
     1201 agcgcgatct gtcgcacccc atcgtatccg tctcgctcgg tgtgccggcg gtgttcttat
     1261 ggggcggcct gcgccgcgcc gaccgccccg cacgcgtgct gctgtttcat ggcgacgtgg
     1321 tggtgtgggg cggtgtcgac cgcctgcgct ttcatggcgt ggcgccggtt cgcctatcaa
     1381 cgcacgcgct gacaggtggt gagcgcatca acttcacgct tcgccgcgcg ggcaacgcgc
     1441 cagacccaat ggtctcatcc aattgacaac caatcgctgc ccagctccaa accgcgtcac
     1501 ccttggcgcg atctgtcagc gcattccaaa ccgggggttc tcatccaaac accccaccgg
     1561 agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgccgc agtcaacgtg
     1681 ccactgttgt tgctgctcgg tgcgccggtg gccgccgtgc tggcctacac gctgctggcc
     1741 aacgtcgtca gcgtcttcag ccacgccaat gttggcctcc cgatgctgga acacgccctg
     1801 cggcacattc tggtaactcc ggcgtttcac cgtgtccacc acgcggcaga ccggccatgg
     1861 accgaccgca acttcgccac tgtctttccg atcttcgacc gcctgctggg caccgccgcc
     1921 accttgccag cgcaggtgca gcagtcgata acgctggggc tggaacattt ccgctcagcg
     1981 ccaaagcaac agctcggagg cttgctcgcc cagcccttcg tcgcgccagg tgaacgcagc
     2041 cagcccccgc gcaagagagc gcccacggcc gacgtgtcta cttgaccccg gattcgggcg
     2101 gccggtccgg cggtggcttg cgccgcgtgt cgtcgaagaa cagcatgaca accaacacat
     2161 agagcagcgg cgccagcacc agcgccacca cgaaggccgc ggcggcgttg acccaaccgc
     2221 cgtagtacag cgcggcgtag atgccgatga aggccagccc tatccacagc ggccagagct
     2281 tcaccacccg cgccacctgc cgcgaaggct gccgggagcc gcaaacctgg agcccataat
     2341 caaagcgcag gcacgcccag ttcgcccagc accttctcga gctgcacggc ggaaaccggg
     2401 gccgggtgcg acgtgacatt gcccgtgggc gagctgtacg gatacgggta gtaccgagtg
     2461 acggacggct gatagctggc gtcgtcgccg gcggtggcgc cgaatttgcc tacgtcgccc
     2521 tcgagccggg tcgccgttgc atgcagccgg tccttcaact gcgtcagctt ggcgggatcc
     2581 atgtggttga tccagtcctg gaacttggcc tgatcggcac cgttttgcag gttcaagcct
     2641 tgcgtttgcg catatttcac cagcaggggg accgcactgt agccgccgcc gctctggtca
     2701 cacagcgttc tcgcggcatc gtcggcgatg cctgcctgct tgaggaaagc cagcgagcgg
     2761 cccttgtcga agtcgggctc gtgctggctg ttggctttgt gatcgttcca cgcccacatg
     2821 ccgacagccg ccaccgccat gatgcccacg cccacccaga cgatcgggcc tcccagcgcg
     2881 gcggcgcccg ccacgcccgc cgcctcgaga cccgaggcgg ccgaggcgcc gaaggtggcc
     2941 atcatcatgc cggtgccctg cgccacgtac aagccaccgg acacgttgtc gcccttggaa
     3001 aacgcgtcgt aagcggcaat ggcatccacc gtggcgccca tcacgccggt gattttcgag
     3061 aacgtactgc cgcccagcca gccgagcttg gactccgctt tcagcacgcc cgtgcccacc
     3121 ccgatctcgg ccccgcgcca gaccaggccc gcagcgtcag acaacacgcg caggttgttc
     3181 gccagcgtcg ggtcgtcgat cgccttggca cccgaggcca tgagccccac gccggccagg
     3241 ccgacaccga ccgcgcgcag catctgcccc ggaacggtgg acttttcaaa gcctttgata
     3301 tcgcccagct cctggtcgac agtgtgcagg cgcttgacca ggctctgcgt gtcgtcgccg
     3361 agcttgggca gcgcgccgtt ttcgatcgcg tcgacggcct tgttcatctg ctcgggcgac
     3421 acgcccatca gcttggcaaa gcgcgaatcc cgcatgctgg ccagcgcatc cttggccttc
     3481 tggatgctgg ccggatcgtt gcggtccagc ccctccagcc ggcccagcac gttggacttg
     3541 acgtaggcgt tggcaaactc ctgacccagc cagacgctgc ggttggccag cttggccggc
     3601 ccgctcaggt tggggttgac gaggaagtcc agcacgcgct ggcccatggg ccccgacgcg
     3661 aggttcgggt cggcctgcag cgcggtagag acagccgcgc gcgaggcggg gtcgtcgagg
     3721 gtctttttca gcgcttcgac gctcgcatcg ctggtggcgc cgttgttggc gccctgcccc
     3781 agcgaagcca tttgcgccag cagcttcttg ccgtcgtccg cgagcgtctg ctgcatgcgc
     3841 ttgccgtcgg cctcccagtt gggatcgtcc ttcagcttgt tggtggtgta gtcggcgatc
     3901 gccttcttca gctgatcggg cgtcatggtg ccgccctggt tctggatcag ccagccgatc
     3961 tcctccatgt gcccggcata ggcgcgcgcg tcttccgtga ctttttcgcg gaactgggtg
     4021 gtgccggtct cgatcacgtg cttcgagatc gcggcatagt cgccgtagag gccctccttc
     4081 ttcggctgct ggccgaaggc gatggcgtag tcgggccggc cgccttcggc gatgtgctgc
     4141 gtcaccgccg gcgcattcca catgcccatc ttgagcatgc ggtccatgtt ggccttgccg
     4201 gtcggcgtgt cgaggctgcg ctccagcact ttgagcgtgg tgcccatctc cttttcgccg
     4261 atgacggggg tcttgtacgt gtcctgatag accttggcgt actgctccag cttgggtagc
     4321 gacttgtcca ccacctgcgc cgccagttcg ggatcgaggc ccttcgtcac ctgatcgagc
     4381 cgctctgcgg cacgctgccc ctccatccgc tcgcccatca tgtagccggg gta
//
LOCUS       DAOMVO010000206         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5747_length_4367_cov_16.657931, whole genome shotgun sequence.
ACCESSION   DAOMVO010000206 DAOMVO010000000
VERSION     DAOMVO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_4367_cov_16.657931"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKV30_09870"
     CDS             <1..532
                     /locus_tag="PKV30_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30551.1"
                     /translation="KLYRRNPAEWRKSAATREAALAEVEKAMAADTPWPPLAGKRDVA
                     ALTLALSPDFAGDRVAAFIVACNDTIVTAHGGRRDFYYLDSIDPQHIYNAARNMETAL
                     WILNSRRDARGAPLLLSNEITADSRNLSFEREFGKIIGRLDLLASTMTERYRRAAISY
                     VQGLALGPVLAFLPVR"
     gene            complement(874..2409)
                     /locus_tag="PKV30_09875"
     CDS             complement(874..2409)
                     /locus_tag="PKV30_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HRL30552.1"
                     /translation="MPQLKEDPAQGTATTPVPDFELSRLAPSRPAHPLWQRLANHPLD
                     DPRDARPLSARLMQSTGWRAAHTQRVLTEYRRFLYLAVRLQGRVCPSEAVDEAWHAHL
                     LDSRRYFGDFCPRVLGYTLHHVPSRGGAEEGARHASRYRATLLAYQHVFGEAPPADIW
                     PAPEARFKERTRHLNSRTHWAVPKPTEWLSASRTSAARAWAIALSLPALLVLGCTQGR
                     VTIEPGTSGPDFLKLYLLALIALAAFGFCRAARGRPAAVDVVRTTGLQAVDHAYVAGG
                     TQRAVQTALASLLQRGAISVSDEAASPGSAWRTHAPPPAGAPPLEHAVFDALRRGFAP
                     DKLAEQVQPALDALHEQLHARGLLTSPRRDERLRDPVDRAHHLLWAGLLVWGVLRILH
                     GTQRGLPVVLLVILTVVGGLLLAFFRANLPHRRTREGRDLVRRATLALRRRSNLGLAD
                     PLMPMALALIGASALGLEMQAFREAIAPMNRSDGSGSSGCGGSSDGGGDGGGGCGGCG
                     GGD"
     gene            2659..>4367
                     /locus_tag="PKV30_09880"
     CDS             2659..>4367
                     /locus_tag="PKV30_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRL30553.1"
                     /translation="MSDLTFDYIIVGAGTAGSLLANRLSRNKSKRVLLIEAGRKDDYH
                     WVHIPVGYLYCIGNPRTDWLYNTEPAAGLNGRTLRYPRGKVLGGCSSINGMIYMRGQS
                     RDYDQWAQLTGEDEWRWEHCVPDFKQHEDHYKLDSASRSPDKRQRPISSNFADLHGHG
                     DRNDAIAAWHGHGGEWRVEKQRLRWDVLDAFSAAAQEAGIPATDDFNRGSNEGVGYFE
                     VNQKAGWRWNTAKAFLRPTCYGRPNFELWTSAQVARLVVERDDSGLRCSGAQVWTGDE
                     MTTAQLAPGGELILAAGSIGSPQILQLSGIGPAGLLQQHGIQVAADLPGVGANLQDHL
                     QIRSVYKINGGKTLNQLASTLWGKGLIGLEYAWRRSGPMSMAPSQLGAFTRSSPAFEW
                     PNIEYHVQPLSLDAFGEPLHAFPAITASVCNLNPTSRGTVHIKSARFEDAPAIAPNYL
                     ATDEDRRVAADSLRVTRRIMAQPAMARFKPEEFKPGVQYQTDEELARLAGDIASTIFH
                     PVGTTKMGRDDDPMAVLTPRLQVRDGANGLIAGLRVVDAGAMPTITSGNTNSPTLMMA
                     EKAA"
BASE COUNT          718 a         1529 c         1473 g          647 t
ORIGIN      
        1 caagctgtac cgccgcaacc cggccgaatg gcgcaagagc gccgccacgc gcgaagccgc
       61 actggccgag gttgagaagg ccatggccgc cgacaccccg tggccgccgc tggcgggcaa
      121 gcgcgacgtg gcggcgctga cgctggcgct gtcgcccgac tttgccggcg accgcgtggc
      181 ggccttcatc gtggcgtgca acgacaccat cgtcaccgcg cacggcggcc ggcgcgactt
      241 ttactacctc gacagcatcg acccgcagca catctacaac gccgcgcgca acatggaaac
      301 cgcgctgtgg attttgaatt cgcggcgcga tgcgcgcggg gcaccgctct tgctgtcgaa
      361 cgagatcacc gccgacagcc gcaacctgag cttcgagcgc gaattcggca agatcatcgg
      421 ccggctcgat ttgctggcca gcaccatgac cgagcgctac cgccgcgcgg ccatcagcta
      481 cgtgcagggg ctggcgctgg ggccggtgct ggcgtttctg ccggtccgtt gatcgcccct
      541 gagtcggcct gcggccgcct tccccccagg gggacaacgc tggcgctcgg ggggaccccg
      601 agcgcggcgt tcccgcgtgg cctgctccgc ggccgtttga aagcgcctgc tgcgcggcgc
      661 tggggaaatc ctgatttggt agcggattgc gcctgataga tgggcgctgg cggcctgttt
      721 ggttcaaatt tctttgatcg aatgacggtt ttgtgttgcg gtcccgactg cgtcacggtc
      781 atgcaaattg tctgaacgct tccttgaatt cactattcat tcgatagctg ttcgcgctga
      841 tccatcaagc gccagaagcc caaacgacac cattcaatca ccgccgccgc acccaccgca
      901 gcccccgccc ccgtcgccac cgccatcgct cgagccgccg cagccgctgc tgccgctgcc
      961 gtcgctgcgg ttcatgggcg cgatggcctc gcgaaaggcc tgcatctcaa gccccagggc
     1021 cgaggcgccg atcagcgcca gtgccatcgg catcagcggg tcggccagcc ccaggttgct
     1081 gcgccggcgc agcgccaggg tggcgcggcg caccagatcg cgcccttcgc gcgtgcggcg
     1141 gtggggcagg ttggcgcgga agaacgccag cagcaagccg cccacgaccg tcaggatgac
     1201 gagcagcacc accggcaggc cgcgctgcgt gccgtgcagg atgcgcagca cgccccagac
     1261 gagcaaaccc gcccagagca gatggtgggc gcggtccacc gggtcgcgca ggcgctcgtc
     1321 gcggcgtggc gaagtgagca ggccgcgcgc atgcagctgc tcgtgcagcg cgtccagcgc
     1381 gggctggacc tgctcggcca gcttgtcggg cgcaaagccg cgccgcaggg cgtcgaacac
     1441 cgcgtgctcc agcgggggcg cgcccgcggg cggcggcgcg tgcgtgcgcc aggcgctgcc
     1501 gggcgatgcc gcctcgtcgc tcacgctgat cgcgccgcgc tgcagcaggc tggccagcgc
     1561 ggtctgcacc gcgcgctgcg taccgccggc cacataggcg tggtccaccg cctgcaagcc
     1621 cgtggtgcgc accacgtcca ccgccgccgg ccgcccgcgc gcggcgcgac agaagccaaa
     1681 ggctgccagt gcgatcaggg ccagcaggta cagcttgaga aagtccgggc ccgaggtgcc
     1741 gggctcgatg gtgacgcgac cctgcgtgca gccaagcacc agcagcgccg gcaggctgag
     1801 cgcgatggcc cacgcgcgcg ccgcgctggt gcgggacgcg ctcagccact cggtgggctt
     1861 gggtacagcc cagtgcgtgc ggctgttgag gtggcgcgtg cgctctttaa agcgcgcttc
     1921 gggcgcaggc cagatgtcgg ccggcggcgc ctccccaaac acgtgttgat aggccagcag
     1981 cgtggcgcgg tagcggctgg cgtggcgcgc gccttcttcg gcgccgccgc gactgggcac
     2041 gtggtgcagc gtgtacccca gcacgcgtgg gcagaagtcg ccaaagtagc gcctcgaatc
     2101 gagcaggtgc gcgtgccagg cttcgtcgac cgcttcgctg ggacacacgc ggccctgcag
     2161 gcgcacggcg aggtacagaa agcggcggta ttcggtcagc acgcgctgcg tgtgcgcggc
     2221 gcgccagccg gtgctttgca tgaggcgcgc gctcaagggg cgggcgtcgc gcgggtcgtc
     2281 gagcgggtgg tttgccaagc gctgccagag cgggtgcgcc gggcggctgg gggccaggcg
     2341 cgagagttcg aagtctggca ctggcgtggt ggcggttccc tgagccgggt cttccttcag
     2401 ttgtggcatg ctgccttctc ctttcttgtt gttgttctga tctacttctt cttattgacc
     2461 gccagattag ggctgaaatg tgacgaaacg gtgaacgtca tttttctgcc ttgatcatgc
     2521 gacaaggcat gcagagcccg ccatgagcga caacaccttc gactacatca tcgtcggcgc
     2581 cgggcgcctt gaagccgcag ggcgaggggc cccggcgcag ccggggatcg acccggcgga
     2641 aaggatgcgt gccggcgtat gagcgacctg actttcgact acatcattgt gggagccggc
     2701 accgccggca gcctgctggc caaccgcctc tcgcgcaaca aatccaagcg cgtgctgctg
     2761 atcgaagccg gccgcaagga cgactaccac tgggtgcata tccccgtcgg ctacctgtac
     2821 tgcattggca acccgcgcac cgactggctc tacaacaccg aacccgccgc cggcctgaac
     2881 ggccgcacgc tgcgctaccc gcgcggcaag gtgctgggcg gctgcagcag catcaacggc
     2941 atgatctaca tgcgcggcca gtcgcgcgat tacgaccagt gggcccagct cacgggcgag
     3001 gacgaatggc gctgggagca ctgcgtgccg gacttcaagc agcacgagga tcactacaaa
     3061 ctggatagcg cctcgcgctc gccagacaag cgccagaggc ctatttcatc caatttcgct
     3121 gatcttcacg gccacggcga ccgaaatgac gcgatcgccg cgtggcacgg ccacggcggc
     3181 gagtggcgcg tcgaaaagca gcgcctgcgc tgggacgtgc tcgacgcctt ctctgccgcc
     3241 gcgcaagagg ccggcattcc cgccaccgac gacttcaacc gcggcagcaa cgaaggcgtg
     3301 ggctatttcg aagtcaacca gaaagccggc tggcgctgga acacggccaa ggcctttctg
     3361 cgcccgacct gctacggccg cccgaacttc gagttgtgga cgtcggcgca ggtcgcgcgc
     3421 ttggttgtag agcgcgatga cagcggcctg cgctgcagcg gcgcgcaggt gtggacgggc
     3481 gacgaaatga ccacggcgca gctcgcgccc ggcggcgagc tgatcctggc cgccggcagc
     3541 atcggctcgc cgcaaatcct gcagctgtcg ggcatcggcc cggccggctt gctgcagcag
     3601 cacggcattc aggtcgcggc ggacctgccc ggcgtcggcg ccaatttgca agaccatttg
     3661 cagatccgca gcgtctacaa gatcaacggc ggcaagacgc tcaaccaact ggccagcacg
     3721 ctgtggggca agggcctgat cgggctcgaa tacgcttggc gccgcagcgg ccccatgagc
     3781 atggcgccca gccagttggg cgccttcact cgctcgtcgc cggccttcga gtggccgaac
     3841 atcgaatacc acgtgcagcc gctcagcctg gacgcgtttg gcgagccgct gcacgccttc
     3901 ccggccatca ccgccagcgt gtgcaacctc aacccgacca gccgcggcac cgtgcacatc
     3961 aagagcgcgc gctttgaaga cgcccccgcc atcgcgccca actacctggc gaccgatgaa
     4021 gaccgccgcg tcgccgccga cagcctgcgc gtcacgcgcc gcatcatggc gcagcccgcc
     4081 atggcgcgct tcaagcccga agagttcaaa cccggcgtgc aataccagac cgacgaggaa
     4141 ctggcgcgcc tggccggcga catcgccagc accatcttcc accccgtcgg caccaccaag
     4201 atggggcgtg acgacgaccc catggccgtg ctcaccccgc gcctgcaggt gcgtgacggc
     4261 gccaacggcc tgatcgccgg cctgcgcgtg gtcgacgccg gcgccatgcc caccatcacc
     4321 agcggcaaca ccaacagccc gacgctgatg atggcggaga aggcggc
//
LOCUS       DAOMVO010000207         4337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5811_length_4337_cov_12.498832, whole genome shotgun sequence.
ACCESSION   DAOMVO010000207 DAOMVO010000000
VERSION     DAOMVO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_4337_cov_12.498832"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKV30_09885"
     CDS             complement(<1..252)
                     /locus_tag="PKV30_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30554.1"
                     /translation="MSDSPPSADHGVPPGAPAPSRGFNLSRWALEHSALVRYLMVVLL
                     LLGVAAYFQLGQDEDPPFTFRVMVVRTNWPGATAQQVAEQ"
     gene            complement(267..1457)
                     /locus_tag="PKV30_09890"
     CDS             complement(267..1457)
                     /locus_tag="PKV30_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL30555.1"
                     /translation="MPASAAWPRGARLWGVGLLAAAAFLGGCSKPPPAEEPVRAVKLL
                     TVGESTYGSGYEYAADMRPRVEARLGFRVAGKITRRAVELGQRVQAGQLLAQVDATDY
                     QLAADAARAQVSSATTQRDLAAADLKRFQGLRDQGFISAAEIERRTAQLKAAQATLDQ
                     ARAQLSSQGHQAGYTQLLADAPGVVTGIEAEVGQVVSAGTPVVRVAQDGPRDVVFAVP
                     EDRVAGVRAGQVVDVRAWASDVRWKGTVREVAASADPVTRTFTVKVALAGDAPPLGAT
                     VYVLPRQSGAQGVPVIKLPTSALRQSGQGSAVWVFDAASGTVQSQPVEVATADGNEVV
                     ITAGLKPGMQVVATGVHVLSPGQKVSVYKPNMPPAQAGQALAAMNSEAASAPAAAAAP
                     AASR"
     gene            1617..>1723
                     /locus_tag="PKV30_09895"
     CDS             1617..>1723
                     /locus_tag="PKV30_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HRL30556.1"
                     /translation="MSAHRPAPIDHYENFPVASWLCPPRLRPPIAAIYH"
     assembly_gap    1724..1823
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1824..2327
                     /locus_tag="PKV30_09900"
     CDS             <1824..2327
                     /locus_tag="PKV30_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRL30557.1"
                     /translation="ADWPELFDYCRRSANPVGRLLLHLYGVTDAQAQQESDAICTALQ
                     LINFWQDLSVDIPRGRYYLPLAECAVHGIVISDPARQFATLKSDEKTTNLIAACAHQA
                     SVSMQKGLKLVHRIPGRAGWELRAVTQGATRVLDKIDALGAAVLHQRPTVGKVDAPRI
                     LWRMLRM"
     gene            2385..3563
                     /locus_tag="PKV30_09905"
     CDS             2385..3563
                     /locus_tag="PKV30_09905"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRL30558.1"
                     /translation="MTTSHFQPLHAALQAQVQRDFLPGVSTALLRGREVVDRFVCGHA
                     DIEASTPLREDHLFRVFSNTKLVTSCAVLLLVEQGLLTLDDPIERYLPELAQRQVLRP
                     GATDLGDVEPARQPITVRHLMTHTAGLSYGLFNPGALLNDAYRQARVHRPTLSNAQLV
                     SELAALPLAFHPGEQWEYSLATDVLGRLIEVTSGETLGRFFSRRIFEPLDMADTAFVL
                     NDEQDRRLATLYAGADRADPTVPGLQRLDDVPYPGAYRTPGMREAGGGGLISSLADTV
                     RLLQALMPGGPTLLKAETLADMARNHLAPGLCVRFPDAPVNPGRVFGLGSAVLAQPGP
                     LDPPGAAGEVNWGGMAGTLWWINPRLGIAGVLMTQRYLGFGDPYTVEFKRLAYQALGH
                     "
     gene            3595..>4337
                     /gene="hpnD"
                     /locus_tag="PKV30_09910"
     CDS             3595..>4337
                     /gene="hpnD"
                     /locus_tag="PKV30_09910"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HRL30559.1"
                     /translation="MSDSSSASPQDYVQQKAAASGSSFYYAFLFLPPPRRAAITAFYA
                     FCREVDDVVDEVSDPGVAATKLAWWQTEVARAYAGEPTHPVMRALMPCAAGYGIEARH
                     LLDIIAGCQMDLTQTRYLDVAALEKYCHLVAGVVGEVSARIFGQTQEKTTEYAHKLGL
                     AFQLTNIIRDVGEDARRGRIYLPLDELQRFDVKAHEILKGTYSDRFAALMQFQAGRAQ
                     RFYDEALALLPAADRRSQKPGLMMASIYRT"
BASE COUNT          644 a         1587 c         1365 g          641 t
ORIGIN      
        1 ctgctcggcc acctgctgcg ccgtggcgcc gggccagttg gtgcgcacca ccatgacgcg
       61 gaaggtgaag ggcgggtctt cgtcctgccc gagctgaaag tacgccgcca cgcccagcag
      121 cagcaacacc accatcaggt agcgcaccag cgccgagtgc tccagcgccc agcgcgacag
      181 gttgaagccg cgggacggcg cgggcgcgcc gggcggcacg ccgtggtcgg cggaaggcgg
      241 ggaatcactc atcgcgcgcc ccgtgttcag cgcgatgccg ccggtgcagc cgcagcagcc
      301 ggcgccgacg ctgcttcact attcatagct gctagcgctt gtcccgcctg cgctggcggc
      361 atatttggct tgtaaaccga gaccttctgc cccggcgaca gcacatgcac gccggtcgcc
      421 acgacctgca tgccgggctt caggccggcg gtgatgacca cctcgttgcc gtcggccgtg
      481 gccacctcca ccggctgcga ttgcacggtg ccgctggccg catcgaacac ccacaccgcc
      541 gagccctgcc ccgactggcg cagcgcgctg gtcggcagct tgatgacggg cacgccctgc
      601 gcgccgctct ggcgcggcag cacgtacacg gtggcgccga gcggcggcgc atcgcccgcc
      661 agcgcgacct tcaccgtgaa ggtgcgcgtg accggatcgg cgctggccgc cacctcgcgc
      721 accgtgccct tccagcgcac gtcgctggcc caggcccgca cgtccaccac ctggcccgcg
      781 cgcaccccgg ccacacggtc ttccggtacg gcaaacacca cgtcgcgcgg gccgtcctgc
      841 gccacgcgca cgaccggcgt gccggccgac acgacctggc cgacttcggc ctcgatgccc
      901 gtcaccacgc ctggcgcgtc ggccagaagt tgcgtgtagc ccgcctggtg gccctggctg
      961 ctcagttgcg cgcgcgcctg atcgagcgtg gcctgcgcgg ccttgagttg cgcggtgcgc
     1021 cgctcgatct cggcggcgct gatgaagccc tggtcgcgca aaccctgaaa gcgcttcaag
     1081 tcggccgccg ccaggtcacg ctgcgtggtc gcgctgctga cctgcgcgcg cgccgcatcg
     1141 gcggccagct ggtaatcggt ggcatcgacc tgcgccagca actggccggc ctgcacgcgc
     1201 tggcccagtt ccaccgcgcg ccgcgtgatc ttgccggcga cgcgaaaacc caggcgcgcc
     1261 tcaacgcgcg ggcgcatgtc ggccgcgtat tcgtaaccgc tgccataagt gctttcgccc
     1321 accgtgagca gcttgaccgc gcgcaccggc tcctcggccg gcggcggctt gctgcagccg
     1381 cccagaaagg cagccgccgc cagcagaccc acgccccaca ggcgcgcgcc gcgcggccac
     1441 gctgcgctgg cgggcacggc gtccctcgac ttgtttcgca tgtacttaag ttaatgactg
     1501 actggttagt aatctaaaca cctgccccgg ccgtgtcaag ctgattgcgg ccggcgtggc
     1561 gccccgcagg cacaatcgcc gcgtcacgca gcgcctgcca gcgtgccgcc accgccatgt
     1621 ccgcgcaccg ccccgctccc atcgatcact acgagaattt cccggtcgcg tcgtggctgt
     1681 gcccgccgcg cctgcggccg cccatcgcgg cgatctacca cttnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnngccgact ggcccgagct cttcgactac tgccgccgct
     1861 cggccaaccc ggtcggccgc ctgctgctgc atctgtacgg cgtgaccgat gcgcaggcac
     1921 agcaagagag tgacgccatc tgcaccgcgc tgcaactcat caacttctgg caagacctga
     1981 gcgtggacat tccgcgcggc cgctactacc tgccgctggc cgaatgcgcc gtgcacggca
     2041 tcgtcatcag cgacccggcg cggcagttcg ccaccttgaa gtccgatgaa aaaactacaa
     2101 acttgatagc tgcttgcgca caccaggcca gcgtcagcat gcaaaaaggc ttgaaactgg
     2161 tgcaccggat tcccggccgc gcggggtggg aactgcgcgc cgtgacgcaa ggcgccacac
     2221 gcgtgctgga caagatcgac gccctgggcg ccgccgtgct gcaccagcgg cccacggtcg
     2281 gcaaggtcga cgcgccgcgc attctgtggc gcatgctgcg catgtagcgt caagccggcg
     2341 cgcggcaggc agaatcgccc acccgtccct ttcgacccca gcccatgacc accagccact
     2401 tccaacccct gcacgccgcc ttgcaggcgc aggtgcagcg cgactttctg cccggcgtgt
     2461 cgaccgcctt gctgcgcggg cgcgaggtgg ttgaccgctt tgtctgtggc cacgccgaca
     2521 tcgaagcgag cacaccgctg cgcgaagacc atttgtttcg cgtcttctcc aacaccaagc
     2581 tggtcacctc gtgcgccgtg ctgctgctgg tggagcaagg cctgctgacg ctggacgacc
     2641 cgatcgagcg ttacctgccc gaactcgccc agcgccaggt gctgcgccca ggcgccaccg
     2701 acctgggcga tgtcgagccg gcacgccagc ccatcaccgt ccgccacctg atgacgcaca
     2761 ccgccggcct gtcttacggc ctgttcaatc cgggcgcgct gctcaacgac gcctaccgcc
     2821 aggcgcgcgt gcaccgcccc acgctgtcca acgcgcaact ggtgagcgag ctggcggcgc
     2881 tgccgctggc ctttcacccc ggcgagcaat gggaatattc gctggccacc gacgtgctgg
     2941 gccggctgat cgaggtgacc tcgggcgaga cgctgggccg cttcttctcg cgccgcatct
     3001 tcgagccgct ggacatggcc gataccgcct tcgtgctgaa cgacgagcaa gaccgccggc
     3061 tggccacgct gtacgccggc gccgaccgcg ccgacccgac cgtgcccggc ctgcagcggc
     3121 tggatgacgt gccctacccc ggcgcctacc gcacgcccgg catgcgcgaa gcgggcggcg
     3181 gcggcctgat ctcgtcgctg gccgacacgg tgcgactgct gcaggcgctg atgcccggcg
     3241 ggccgacgct gctcaaggcc gagacgctgg ccgacatggc gcgcaaccac ctcgcgcccg
     3301 gcctgtgcgt ccgctttccc gatgcgccgg tcaaccccgg ccgcgtgttc ggcctgggct
     3361 cggccgtgct cgcacagccg ggcccgctcg atccgcccgg cgcggctggc gaagtcaact
     3421 ggggcggcat ggccggcacg ctgtggtgga tcaacccgcg ccttggcatc gccggtgtgt
     3481 tgatgaccca gcgctacctg ggctttggcg acccgtacac cgtcgaattc aaacgcctcg
     3541 cctaccaggc gctggggcac tgagccgtca tgggcgaatg ggacaatcct gggcgtgagt
     3601 gactcttctt ccgcatcgcc gcaagactac gtgcagcaaa aagccgccgc atccggcagc
     3661 agcttttatt acgcgttcct gttcctgccg ccgccgcgcc gcgccgccat caccgcgttc
     3721 tatgccttct gccgcgaggt ggacgacgtg gtggacgaag tcagcgaccc cggcgtggcc
     3781 gccaccaaac tggcctggtg gcaaaccgag gtggcccgcg cctacgcggg cgagccgacg
     3841 cacccggtga tgcgcgcgct gatgccctgc gcggccggct acggcatcga ggcgcggcac
     3901 ctgctggaca tcatcgccgg ctgccagatg gatttgacgc agacgcgcta cctggacgtt
     3961 gccgcgctgg agaaatactg ccacctggtc gccggtgtgg tgggcgaggt ctcggcgcgc
     4021 atcttcggcc agacgcagga aaagacgacc gaatacgccc acaagctggg cctggcgttt
     4081 cagctcacca acatcatccg cgacgtcggc gaagacgcgc ggcgcgggcg catctacctg
     4141 ccgctggacg agttgcagcg ctttgacgtc aaggcgcacg agatactcaa aggcacgtat
     4201 tcagaccgct tcgcggcgtt gatgcagttc caggccggac gcgcgcagcg cttttacgac
     4261 gaggcgctgg ccctgctgcc cgccgccgac cgccgcagcc agaagccggg cctgatgatg
     4321 gccagcatct accgcac
//
LOCUS       DAOMVO010000208         4326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5827_length_4326_cov_13.115196, whole genome shotgun sequence.
ACCESSION   DAOMVO010000208 DAOMVO010000000
VERSION     DAOMVO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5827_length_4326_cov_13.115196"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKV30_09915"
     CDS             <1..818
                     /locus_tag="PKV30_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRL30560.1"
                     /translation="PYMGRAAGPMTRSVADAALMMQVLAQPDARDSMALPPQAIDWSL
                     AWRAGKPLKGVRIGLLLNAGCGLPVEPAIRVAALHAAHLFEGQGAVVTALAPFMTQEL
                     LAGLDHFWRMRSLVDLDALPAERRASVLPVIRDWADSARAMSGADVFRAYSATHAVRV
                     ATQAALQPFDYVISPVSPNAPAPFDWPSPTNDPLRGLEHIGFTVPFNMGEQPAISVNC
                     GTMDSPGTPGAALPIGLQIAGQRFDDLGVLQMARVFEQLRGPQRDWPQPPMTK"
     gene            951..1805
                     /locus_tag="PKV30_09920"
     CDS             951..1805
                     /locus_tag="PKV30_09920"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HRL30561.1"
                     /translation="MTFPLNADLHCHSTTSDGTLTPEQLAARAAANGVDLWALTDHDE
                     VGGQQQALEAARAQGMKYVTGVEISVTFADVTVHIVGLGFDHTDPRMMEGLADTRDGR
                     GPRAVEMGAQLAKVGIEGAYEGALKYVGNPRLISRTHFARFLVDSGVCRDTSEVFRKF
                     LTEGKPGYVPHRWAKLGDAVKWITQSGGIAVIAHPARYKFTPNEEYALFSEFKQHGGQ
                     GVEVVTGSHTAAEAVRYADMAREFGLAASRGSDFHSPDESHVDLGKLPPLPADLTPVW
                     SLLTDRAH"
     gene            1877..2500
                     /locus_tag="PKV30_09925"
     CDS             1877..2500
                     /locus_tag="PKV30_09925"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRL30562.1"
                     /translation="MAQHFSVHPDNPQPRLLKQAAALLTQGGVLAVPTDSSYALACQL
                     DDKAAADQLRRIRQVDDKHHLTLLCRDLRELANYARVDNAQFRLLKNATPGPFTFILE
                     ASKEVPRRVSHPQRKTIGLRVPEHPALQALLEVHGAPLLATTLILPGETEPLNDPEEI
                     LERLEKQIAGVIDSGACPLEPTTVVDLSGDAPEVLRRGRGDPAALGL"
     gene            complement(2505..2672)
                     /locus_tag="PKV30_09930"
     CDS             complement(2505..2672)
                     /locus_tag="PKV30_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30563.1"
                     /translation="MTRLAAHDFGFAFNDGAARHDVRGQGHFVIQAQRSHFVIKTGAS
                     ACQSSAGSYGF"
     gene            2759..3427
                     /locus_tag="PKV30_09935"
     CDS             2759..3427
                     /locus_tag="PKV30_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRL30564.1"
                     /translation="MDFANIIQTIAIYALPVLFAITVHEAAHGYVARHFGDGTAAFMG
                     RVTLNPLKHIDPIGTIAMPLFLYIATGGAFLFGYAKPVPVNFGALRNPRRDSIWVALA
                     GPGSNLVQALGWGALLYVLRGAGVEERFFLEMCRAGVLVNVIMFVFNLFPLPPLDGGR
                     ILTSLLPPRLAIPFSRIEPWGFFIVMGLIVAGVISKLWMLPLMSLTFSLLDLLLSPLA
                     ALLS"
     gene            3443..>4326
                     /gene="trpS"
                     /locus_tag="PKV30_09940"
     CDS             3443..>4326
                     /gene="trpS"
                     /locus_tag="PKV30_09940"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRL30565.1"
                     /translation="MSAKEPTRVLTGITTTGTPHLGNYVGSIRASVRASLQPGVQSFY
                     FLADYHALIKCDEPARIQRSTLEIAASWLAAGLDPERVTFYRQSDIPEIPELTWFLTC
                     VTGKGLLNRAHAYKAMLDKNAAAGAEADNDVSAGLFMYPVLMGADILMFKAHKVPVGR
                     DQIQHIEMARDMAASFNHLYGQLLVAPEALIDDNVALLPGLDGRKMSKSYDNTIALFG
                     PREQLKKQIMNIVTDSRAPGEPKSTEGSALYQIYRAFADDAEAAAFAQAFADGIAWGD
                     AKQRLFERIDAEIGPMRER"
BASE COUNT          710 a         1514 c         1388 g          714 t
ORIGIN      
        1 cgccctacat gggccgcgcc gccggcccca tgacgcgcag cgtggccgac gccgccctga
       61 tgatgcaggt gttggcacaa cccgacgcgc gcgacagcat ggcgctgccg ccgcaggcca
      121 tcgattggtc gctggcctgg cgggccggca agcccctgaa aggcgtgcgc atcggcctgc
      181 tgctgaacgc cggctgcggc ctgcccgtgg aaccggcgat ccgcgttgcc gccctgcacg
      241 ccgctcatct ttttgaaggg caaggcgccg tcgtgacagc gctggcgccc ttcatgacgc
      301 aagagctgct ggccgggctc gatcacttct ggcgcatgcg ctcgctggtc gatctggacg
      361 cgctgcctgc cgagcggcgc gccagcgtgc tgcccgtcat ccgcgactgg gccgacagcg
      421 cgcgcgccat gtcgggtgcc gacgtgttcc gcgcttacag cgccacgcac gcggtgcgcg
      481 tggccacgca ggcggccctg cagccgttcg attacgtgat ctcgccggtg tcgcccaacg
      541 cgccggcgcc cttcgactgg cccagcccca ccaacgaccc gctgcgcggg ctggagcaca
      601 tcggcttcac tgtgcccttc aacatgggcg agcagccggc catttcggtc aactgcggca
      661 ccatggattc gcccggcacg cccggcgccg cgctgccgat tggcttgcag attgccggcc
      721 agcggtttga cgatctgggc gtgttgcaga tggcgcgggt gtttgagcag ttgcgcgggc
      781 cgcaacgtga ttggccccag ccgccgatga cgaaatgacc tcggcctgcg gccgttgatg
      841 acggcgctgc gcgccatgac caggcgaaag tcgttccgtc attgacgccg cgcgagcggc
      901 caagtcatcg cgcgccagcg cggtcatcaa gcgcttcgta aaatggcccg gtgaccttcc
      961 ccctcaacgc cgacctgcac tgccactcca ccacgtccga tggcacgctc acgcccgagc
     1021 aactggcggc gcgtgcggcg gccaacgggg tcgatctgtg ggcgctgacg gatcacgacg
     1081 aggtcggcgg ccagcagcag gcgctcgaag cggcccgcgc gcagggcatg aagtacgtga
     1141 cgggcgtcga gatttccgtg acctttgccg atgtcaccgt gcacatcgtc ggcctgggct
     1201 ttgaccacac cgacccgcgc atgatggaag gcctggccga cacgcgcgac gggcgcgggc
     1261 cgcgcgcggt cgagatgggc gcgcaactgg ccaaggtggg cattgagggc gcgtacgaag
     1321 gcgcactgaa gtacgtcggc aacccgcgcc tgatctcgcg cacgcatttc gcacgctttc
     1381 tggtcgattc gggcgtgtgc cgcgacacgt ccgaggtgtt tcgcaaattc ctgaccgaag
     1441 gcaagcccgg ctacgtgccg caccgctggg ccaagttggg cgatgcggtg aagtggatca
     1501 cgcaaagcgg cggcatcgcc gtcatcgcgc acccggcgcg ctacaagttc acgccgaatg
     1561 aggagtacgc gctgttctct gaattcaagc agcacggcgg ccagggcgtg gaagtggtca
     1621 ccggcagcca cacggccgcc gaagccgtgc gctacgccga catggcccgc gaattcggcc
     1681 tggccgcttc gcgcggcagc gacttccaca gcccggatga aagccacgtc gacctcggca
     1741 agctgccgcc gctgccggcc gacctgacgc cggtgtggtc gctgctgact gatcgcgcgc
     1801 attgatttca agcgttttag gctgccagcg cttgccaaac aagcgcaagc agctcctgtt
     1861 tttatagttc accctcatgg cccagcactt cagcgtccac cccgacaacc cgcagccgcg
     1921 cctgttgaag caggccgcgg ccctgctcac gcaaggcggc gtgctggccg tgccgaccga
     1981 ttcgagctac gcgctggcct gccagctcga cgacaaggcc gccgccgacc aattgcgccg
     2041 catccgccag gtcgacgaca agcaccacct gacgctgctg tgccgcgatt tgcgcgaact
     2101 ggccaactac gcccgcgtcg acaacgcgca gttccgcctg ctgaagaacg ccacgccggg
     2161 gccgttcacc ttcatcctcg aagccagcaa ggaagtgccg cgccgcgtca gccacccgca
     2221 gcgcaagacc atcggcctgc gcgtgcccga gcacccggcg ctgcaggcgc tgcttgaagt
     2281 gcatggcgcg ccgctgctgg ccaccacgct gatcctgccg ggcgagaccg agccgttgaa
     2341 cgaccccgag gaaatcctgg agcgcctcga aaagcagatt gccggcgtga tcgactcggg
     2401 tgcgtgcccg ctggagccca ccaccgtggt ggatctgagc ggcgatgcgc ccgaggtgct
     2461 gcgccgcggg cggggcgatc cggcggcgct gggcctttga cgcatcaaaa accatagctg
     2521 cccgcgcttg actggcaagc actggcgcct gttttgatga cgaaatgact tcgctgcgct
     2581 tgaatgacga aatgaccctg gcctctgaca tcatggcgcg cggcgccgtc attgaaggcg
     2641 aagccgaagt catgcgccgc caggcgcgtc atcggtgaca acgctgaagc tcattttcaa
     2701 accgaataca ccgccgccga acgcgcctcg cggcaggctc tgagacaatt ccgccctcat
     2761 ggatttcgcc aacatcattc aaaccatcgc catctacgcg ctgccggtgc tcttcgccat
     2821 caccgtgcac gaagcggcgc acggttatgt ggcgcggcac ttcggcgacg gcacggcggc
     2881 cttcatgggc cgcgtcacgc tcaacccgct caagcacatc gacccgatcg gcaccatcgc
     2941 gatgccgctg tttctgtaca tcgccaccgg cggcgcgttt ctgttcggct acgccaagcc
     3001 ggtgccggtc aacttcggcg ccttgcgcaa tccgcggcgc gacagcatct gggtggcgct
     3061 ggcggggccg ggctccaacc tggtgcaggc gctgggctgg ggcgcgctgc tgtacgtgct
     3121 gcgcggcgcg ggtgttgaag agcgcttttt cctcgagatg tgccgcgcgg gcgtgctggt
     3181 caacgtgatc atgttcgtgt tcaacctgtt tccgctgcca ccgctggatg gcgggcgcat
     3241 tcttaccagc ctgctgccgc cacggctggc gattccgttc agccgcatcg agccgtgggg
     3301 ctttttcatc gtcatgggcc tgatcgtggc cggcgtcatc agcaagctgt ggatgctgcc
     3361 gctgatgtcg ctcaccttca gcttgctcga cctgcttctg tcgccgctgg cggcgctgct
     3421 cagctagttt tttccgcttg tcatgtccgc aaaagaaccc acccgcgtcc tcaccggcat
     3481 caccaccacc ggcacgccgc accttggcaa ctacgtcggc tcgatccgcg cctcggtgcg
     3541 cgccagcctg cagccgggcg tgcaaagctt ttactttctg gccgactacc acgcgctcat
     3601 caagtgcgac gagccggcgc gcattcagcg ctcgacactg gaaatcgccg ccagctggct
     3661 ggccgccggg ctcgaccccg agcgcgtgac cttctaccgc cagtcggaca tccctgaaat
     3721 ccccgaactg acgtggttcc tgacctgcgt caccggcaag ggcctgctca accgcgccca
     3781 cgcctacaag gcgatgctgg acaagaacgc ggccgccggg gctgaagcag acaacgacgt
     3841 cagcgccggc ctgttcatgt acccggtgct gatgggcgcc gacatcctga tgttcaaggc
     3901 gcacaaggtg ccggtcgggc gcgaccagat tcagcacatc gagatggcgc gcgacatggc
     3961 ggccagcttc aaccacctgt acggccagct gctggtggcg cccgaggcgc tgatcgacga
     4021 caacgtggcc ctgctgcccg gcctggacgg ccgcaagatg agcaagagct acgacaacac
     4081 catcgccctg ttcggcccgc gcgagcagtt gaagaagcag atcatgaaca tcgtcaccga
     4141 ttcgcgcgcg ccgggcgagc cgaaatcgac ggaaggctcg gcgctgtacc agatctaccg
     4201 cgcctttgcc gacgacgccg aggccgccgc cttcgcgcag gcctttgccg acggcattgc
     4261 gtggggcgac gccaagcagc ggctgttcga gcgcatcgat gccgaaatcg gccccatgcg
     4321 cgagcg
//
LOCUS       DAOMVO010000209         4315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5851_length_4315_cov_16.769953, whole genome shotgun sequence.
ACCESSION   DAOMVO010000209 DAOMVO010000000
VERSION     DAOMVO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5851_length_4315_cov_16.769953"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKV30_09945"
     rRNA            complement(<1..315)
                     /locus_tag="PKV30_09945"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            943..2829
                     /locus_tag="PKV30_09950"
     CDS             943..2829
                     /locus_tag="PKV30_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30566.1"
                     /translation="MALLTLLDAQLAFGHVPLLDHADFALEAGERVGLIGRNGAGKSS
                     LLRILGGLESADDGQLQAQQSLRVAFVAQEPELADDATVFDAVAQSLAPLRALMDSYA
                     AGQGDLDALQERIEAQDGWTWRRRVDETLQRLRLPAEGTIGTLSGGTRKRVALAQALV
                     MRPDVLLLDEPTNHLDLDAIRWLEDLLLDFQGAVVAISHDRAFLDRVATRIVELDRGK
                     LLSYPGNFSRYQQLKQEQLAQEATINARADKLLAQEEVWVRKGVEARRTRAQGRINRL
                     QALRAARLARRDAVGRVRLDVDAGVAGYQGKLVAELDNVSLAFGDKRVVEQLSTTVLR
                     GDKIGLIGPNGAGKTTLLKLILGDIEPDSGRIQRGANLQVAYFDQMRAALDPDATLED
                     FISPGSEWIEIGAKRQHVKSYLGDFLFSPARAASPVRSLSGGERNRLLLARLFARPAN
                     VLVLDEPTNDLDIDTLELLEELLQGYEGTVFLVSHDRTFLDNVVTSTLAWDGPGAWRE
                     YEGGVQDWLAQSARWQAMREAEAGAASDVSDQTSPQRTPDKREQLQKKKLSYKDQREL
                     DALPALIDALEQEQADIRARLADGTLYQTDLDQAVALQARDGVIEEELMAALERWETL
                     SSHS"
     gene            complement(3041..>4315)
                     /gene="glmU"
                     /locus_tag="PKV30_09955"
     CDS             complement(3041..>4315)
                     /gene="glmU"
                     /locus_tag="PKV30_09955"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRL30567.1"
                     /translation="GHGAPEVEAFCADWASAGGRNGTAITPQPVRQEPQLGTGHAVQQ
                     AAPLLPDDGLTLILNGDVPLVRTETMRRLIALCGKDQIALLTVDTGEDAGAYGRIVRG
                     AAEGDGDIPVRAIVEAKDATADQRAITEWYSGVMAAPTRLLKKYLALLTDDNAQKEFY
                     LTDVVKHAVADGTPVLALQIDDEVEVAGVNSPVQLAELEREVQQRAARRLMEAGVRLA
                     DPARFDLRGELDCGQDVEIDVGCIFEGAVTLGDNVRIGAHCVIANAKIAAGAVIHPFT
                     HIDGGAQGVVVGPGALVGPYARLRPGAELGPEVHIGNFVEVKNSTLAAGAKANHLAYL
                     GDATVGERVNYGAGSITANYDGANKHRTVIEADAHIGSNCVLVAPVTIGAGGTVGGGS
                     TITKSTPPGALSVTRAKQVSIADWARPQKQKK"
BASE COUNT          732 a         1444 c         1339 g          800 t
ORIGIN      
        1 agtcccagtg tggctggtcg tcctctcaga ccagctacag atcgcaggct tggtaagcct
       61 ttaccccacc aactacctaa tctgccatcg gccgctccaa acgcgcgagg tcttgcgatc
      121 ccccgctttc atccttagat cgtatgcggt attagctact ctttcgagta gttatccccc
      181 acgtctgggc acgttccgat gtattactca cccgttcgcc gctcgccgcc aggttgcccc
      241 gcgctgccgc tcgacttgca tgtgtaaagc attccgccag cgttcaatct gagccaggat
      301 caaactctac agttcgatct tgaaatttat actcttccga gttaactcat ttaaacggaa
      361 ttgaagtgaa cttcacttct gttttcatga gcgtttgata gtctgaaaga ctttggcaat
      421 tcgccatcaa acgcccacgc ttatcggctg tatattttta atgaaccgtc gcatcaagct
      481 ttgctttctg cgtcacttca gttgcgatca actgagcctt gaattatgac atgattttta
      541 aaaacctgtc aaaccttagg gtttttaccg atttcgcttg gcacgccttg cgatctcgct
      601 tccctcgacc ttaactgcgt tctgggaagc cttgcagtat cgcatgcttt cgaacaacct
      661 gtcaagctct agggtcttta cttcttgcgc tgcctgatca cagatcagag tcagcacctt
      721 ctgcaccccg ccagtaagaa cccgcttgcg ctgcctgtta ccggcttcgt tgaccgctgc
      781 ttggcagcga tcagcgaagc cttagactgt agcacgcttt ttgaacccgc cgcaagggct
      841 tcgaaaaaaa tttttgctgg ctccgcatca ccactgtgac gaccgcaggt ctccacagtg
      901 gtgactgcgg caacaaacct tctttagaac ccatcttctt tcatggccct gctcaccctg
      961 ctcgatgccc aactggcttt tggccacgtg cccctgcttg atcacgcgga ctttgcgctg
     1021 gaggcgggcg agcgcgtggg cctcatcggc cgcaacgggg cgggcaagtc ttcgctgttg
     1081 cgcatcctgg gcgggctgga gagcgcggat gacgggcagc ttcaggcgca gcaaagcctg
     1141 cgcgtggcgt tcgtggcgca ggagcccgag ctggcggacg acgccaccgt gttcgacgcg
     1201 gtcgcccaaa gtctggcccc gctgcgcgca ctgatggatt cgtacgccgc cggccagggc
     1261 gacctggacg cgctacagga gcgcatcgag gcgcaagacg gctggacctg gcgccggcgc
     1321 gtcgacgaaa ccctgcagcg cctgcggctg ccggccgagg gcacgatcgg cacgctgtcg
     1381 ggcggcacac gcaagcgcgt ggcgctggcg caggcgctgg tgatgcggcc cgatgtgctg
     1441 ctgctcgacg agccgaccaa ccacctcgat ctggacgcca tccgctggct ggaagatttg
     1501 ctgctcgact tccagggcgc ggtggtcgcc atctcgcacg accgcgcgtt tctggaccga
     1561 gtggccacgc gcatcgtcga gctggaccgc ggcaagctgc tgtcttaccc cggcaacttc
     1621 agccgctacc agcagttgaa gcaggaacaa ctcgcgcaag aggcgaccat caacgcgcgc
     1681 gccgacaagc tgctggcgca agaggaagtc tgggtgcgca agggcgtcga ggcgcggcgc
     1741 acccgtgcgc aaggccgcat caaccgcttg caggcgctgc gggccgcgcg cctggcacgg
     1801 cgcgacgccg tgggccgcgt gcggctcgac gtcgatgccg gcgtggccgg ctaccagggc
     1861 aagctggtgg ccgagctgga caacgtgagc ctggcgtttg gcgacaagcg cgtcgtggag
     1921 cagctcagca ccaccgtgct gcgcggcgac aagatcggcc tgatcggccc caacggcgcc
     1981 ggcaagacca cgctgctcaa gctgatcctg ggcgacatcg aacccgacag cggccgcatc
     2041 cagcgcggcg ccaacctgca ggtggcgtat ttcgaccaga tgcgcgccgc gctcgacccc
     2101 gacgccacgc tggaagactt catcagcccc ggcagcgagt ggatcgagat cggcgccaag
     2161 cgccagcacg tcaagagcta cctgggcgac tttctgttca gcccggcgcg cgcggcttcg
     2221 cccgtgcgat ccttgagcgg tggcgagcgc aaccggctgc tgctggcgcg cctgttcgcc
     2281 cggccggcca acgtgctggt gctcgacgag ccgaccaacg acctggacat cgatacgctg
     2341 gagctgctgg aggaattgct gcaaggctac gagggcacgg tgttcctcgt cagtcacgac
     2401 cgcacctttc tggacaacgt ggtcaccagc acgctggcgt gggacggccc cggcgcctgg
     2461 cgcgaatacg aaggtggcgt gcaagactgg ctggcgcaaa gcgcgcgctg gcaggccatg
     2521 cgcgaggccg aggccggcgc cgcgtccgac gtttcagatc aaacaagccc ccagcgcacg
     2581 ccagacaagc gcgagcagct acaaaaaaag aagttgagct acaaggacca gcgcgagctg
     2641 gacgccctgc ccgcgctcat cgacgccttg gagcaggagc aggccgacat ccgcgcccgg
     2701 ctggccgacg gcacgctgta ccagaccgac ctggatcagg cggtcgccct gcaagcgcgc
     2761 gacggcgtca tcgaggaaga actgatggcg gcgctggagc gctgggagac gctgagcagt
     2821 cacagctgaa gccctgcact atctgacacc agttggtcct gagcctatcg aaggactggg
     2881 tcatacgaac aggcttcgac aagcctgtca tgagccaagt cgaatggctc agcccgaacg
     2941 gatcggggtg tcgggccgtt tttcgaaaat ggctgcggcc ttttgctata ccaactatag
     3001 ctgctcatgc ttgctgcaca agcgccgggt ggcattttga ttactttttc tgcttctgcg
     3061 gccgcgccca atcggcgatg ctcacctgct tggcacgcgt cacactcaag gcgcccggcg
     3121 gcgtgctctt ggtgatggtg ctgccgccgc ccaccgtgcc gccagcgccg atggtcaccg
     3181 gcgccaccag cacgcagttg ctgccgatgt gcgcgtccgc ctcgatcacg gtgcggtgct
     3241 tgttcgcgcc gtcgtagttg gcggtgatgc tgccggcgcc gtagttcacg cgctcgccca
     3301 ccgtggcgtc gcccaggtag gccaggtggt tggccttggc gccggcggcg agcgtggaat
     3361 tcttcacctc gacgaagttg ccgatgtgca cttcaggccc cagctcggcg cccgggcgca
     3421 gccgcgcata gggcccgacc agcgcgccgg gcccgaccac cacgccctgc gcaccgccgt
     3481 cgatgtgggt aaagggatgg atcaccgcgc ccgcggcgat cttcgcgttg gcaatcacgc
     3541 agtgcgcgcc gatgcgcacg ttgtcgccca atgtgaccgc gccttcaaag atgcagccga
     3601 cgtcaatctc cacgtcctgc ccgcagtcga gttcgccgcg caagtcaaag cgcgccgggt
     3661 cggccaggcg cacaccggct tccatcagcc gccgcgcggc gcgctgctgc acctcgcgct
     3721 ccagctcggc cagttgcacc gggctgttca cgcccgccac ctccacttcg tcgtcgatct
     3781 gcagcgccag caccggtgtg ccatcggcca ccgcgtgctt gacgacgtcg gtcaggtaga
     3841 actctttttg cgcgttgtcg tcggtcagca gcgccaggta tttcttcagc aggcgcgtgg
     3901 gcgcggccat gacgccgctg taccactcgg tgatggcgcg ctggtcggcc gtggcgtcct
     3961 tggcttcgac gatggcgcgc acggggatgt cgccatcgcc ctccgccgcg ccgcgcacga
     4021 tgcggccata ggcgccggcg tcctcgcccg tgtccaccgt cagcagcgcg atctggtcct
     4081 tgccgcacag cgcgatcagg cgccgcatgg tctcggtgcg caccagcggc acgtcgccgt
     4141 tcaggatcag cgtcaggcca tcgtccggca gcagcggcgc cgcctgctgc acggcgtggc
     4201 cggtgcccag ttgcggttcc tggcgcaccg gctgcggcgt gattgcagtg ccatttcggc
     4261 cgccagcgct tgcccagtca gcgcagaacg cttcaacttc aggagcaccg tggcc
//
LOCUS       DAOMVO010000210         4300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5880_length_4300_cov_12.401413, whole genome shotgun sequence.
ACCESSION   DAOMVO010000210 DAOMVO010000000
VERSION     DAOMVO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5880_length_4300_cov_12.401413"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1508
                     /gene="tadA"
                     /locus_tag="PKV30_09960"
     CDS             129..1508
                     /gene="tadA"
                     /locus_tag="PKV30_09960"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRL30568.1"
                     /translation="MSAETPVTDDAHAMRLALAEARAAADAGEVPVGAVVVKDGRVIA
                     TGRNAPIAAHDPTAHAEIVALRAAAESLGNYRLDGCQLFVTLEPCAMCAGAMLHSRLD
                     RVVFGAADPKTGAAGSVVNLFADARLNHGTQVEGGVLAGEGAALLQDFFKPRRINPSP
                     LRDDALRTPAARFADLPGYPWAPHYVSDLPALDGLRMHYLDEGPPDAPRTWLCLHGNP
                     TWSYLYRHMIPVFLAAGDRVVAPDLIGFGKSDKPKKEGAHTFGWHRQVLLELIERLDL
                     QRVTLVVQDWGGLLGLTLPMAAPQRYAGLLAMNTLLATGDAPLPPGFVDWRDWCARNP
                     AFDIGRLMERSNPQLSAAECVAYAAPFADAGHRAGTRAFPRMVPEAIDDGAAALSRAA
                     RDFWRDEWSGRSLMAIGLQDPVLGDAVMQALRRDIRGCPEPLRLAHAGHFVPESGRAL
                     AEHAVIHFS"
     gene            1554..2528
                     /locus_tag="PKV30_09965"
     CDS             1554..2528
                     /locus_tag="PKV30_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRL30569.1"
                     /translation="MSHIYIYSPSGAVRDRAAFRRGVKRLQALGHEVEIDPDALASFE
                     RFAGDDATRLAAIGRAAASGADVALITRGGYGITRLLPDLPYRALARAANKGTRFVGL
                     SDFTALQLALLAKTGATTWAGPALLEGFGAADATDAGEGHGGHATAGGPDEIMLACFD
                     DLVSGVGEGTGWRLPAVDVAALGGHTGSLAHHAPLWGGNLAMVASLVGAPYFPEVKGG
                     VLFLEDVAEHPYRVERMLAQLLHAGVLARQKAIVLGQFTEYKLTPHDRGYSMKKVVDW
                     LRGQLPRTPVLTGLPFGHVPTKVLLPVGAKVDLLVEERDALVLWGHMH"
     gene            complement(2567..2884)
                     /locus_tag="PKV30_09970"
     CDS             complement(2567..2884)
                     /locus_tag="PKV30_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30570.1"
                     /translation="MSALYGRLSRHEPDQARLAHRVDDIRTAMLDMLGHAGAQQYPHV
                     ARRIRFGGDALALWYGRADLMGALAGLHGEQVARTRMVSLSVLFEGTLPKGMMSRPTT
                     LRT"
     gene            3167..4180
                     /locus_tag="PKV30_09975"
     CDS             3167..4180
                     /locus_tag="PKV30_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRL30571.1"
                     /translation="MSSSVTMVFIDLEGSTAAYAALGNAQVADVIAKVTQWIGRVCEA
                     HGGRTVKFLGDGVLAQFPHGADAVEAAVFMQLNHSERLEKWPEPLRMGLKIGMARGSV
                     VQMADDVYGDPVNLAARLSDMAGAQTIWADESVVVQLREGREPSSRETHVDGERAAIE
                     GARYRSLGMMRVRGLSQPHSVFQIEWSEDVSTDLMTVRGALHDIEASAGPVGGSIALA
                     WLGNSKVYTVDDLPFSIGRIPDSGFVVGDQRVSRSHARIEFINGAFVLSDLSSFGTWV
                     RFADNPGEVQLRRNQCVLHSTGEIALGASFSDFSAAVVAFSVGATEAAGRGSSSTRSL
                     FND"
BASE COUNT          697 a         1399 c         1483 g          721 t
ORIGIN      
        1 gaggcgatgg acgccgccat gcagccgcag cgcgccatgc cggacgacgc caagtgaccg
       61 gcgtttgaat caaattggcc tccagcgcag gcaggacaag cgcgagcagc tataaaacaa
      121 agagcatctt gagcgccgaa acacccgtca ccgacgacgc ccacgccatg cgcctggcgc
      181 tggccgaagc gcgcgcggcg gccgacgcgg gcgaggtgcc ggtcggcgcc gtggtggtca
      241 aggacggccg cgtcatcgcc accggccgca acgcgccgat tgccgcgcac gacccaacgg
      301 cgcacgccga aatcgtggcg ctgcgcgcgg cggctgaaag tttgggcaac taccggctgg
      361 acggctgcca gttgttcgtc acgctcgagc cgtgcgccat gtgcgccggc gccatgctgc
      421 actcgcggct ggatcgcgtg gtgtttggcg cggccgaccc caagaccggc gccgccggct
      481 cggtcgtcaa cctgtttgcc gatgcgcgcc tgaaccacgg cacgcaagtc gaaggcggcg
      541 tgctggccgg ggagggcgcg gcgctgctgc aagatttttt caagccgcgc cgcatcaacc
      601 ccagcccgct gcgcgacgac gccctgcgca cgcccgccgc ccgctttgcc gatctgcccg
      661 gctacccgtg ggcgccgcac tacgtcagcg acttgcccgc gcttgacggc ctgcgcatgc
      721 actatctgga tgaaggcccg cccgatgccc cgcgcacctg gctctgcctg cacggcaacc
      781 cgacgtggag ctatctgtat cggcacatga tcccggtctt tctggccgct ggcgaccgcg
      841 tggtggcacc cgatctgatc ggcttcggca aaagcgacaa gccgaagaag gagggcgccc
      901 acaccttcgg ctggcaccgg caggtgctgc tggagctgat cgagcggctg gatctgcagc
      961 gcgtgacgct ggtggtgcag gactggggcg gcctgctcgg cctgacgctg cccatggccg
     1021 cgccacagcg ctacgccggc ctgctggcga tgaacacgct gctcgccacc ggcgacgcgc
     1081 cgctgccccc tggcttcgtt gactggcgcg actggtgcgc caggaacccg gcgttcgaca
     1141 ttggccggct gatggagcgc agcaacccgc aattgagcgc ggccgaatgc gtggcttacg
     1201 cggcgccgtt tgccgatgcg gggcatcgcg ccggcacgcg agccttcccg cgcatggtgc
     1261 ccgaggcgat agacgacggc gccgccgctt tgtcgcgcgc cgcgcgggac ttctggcgcg
     1321 atgaatggtc gggccgcagc ctgatggcca tcgggctgca agacccggtg ctgggcgatg
     1381 ccgtcatgca ggccttgcgc cgcgacattc gcggttgccc cgaaccgctg cgcctggcgc
     1441 acgccggcca cttcgtgccg gaatcggggc gtgcgctggc cgagcacgcg gtgatacact
     1501 tttcatagct gccagagcac gtccaacaag cgctggcgcc gtttttgact gtcatgtccc
     1561 acatctacat ctattcccca tccggcgcgg tgcgcgaccg cgcggcgttc cggcgcggcg
     1621 tcaagcggct gcaggcgctg ggccacgagg tcgagatcga ccccgacgca ctggccagtt
     1681 ttgagcgctt tgccggcgac gacgccacgc gcctggccgc catcggccgc gccgctgcca
     1741 gcggtgccga tgtggcgctg atcacgcgtg gcggctatgg catcacgcgg ctgctgcccg
     1801 acttgccgta ccgcgcgctg gcccgcgccg ccaacaaagg cacgcgtttc gtcggcctga
     1861 gcgatttcac cgcactgcaa ctggccttgc tggccaagac cggcgccacc acctgggccg
     1921 gcccagcgct gctggaaggc tttggcgccg ccgatgccac cgatgcgggc gaaggccacg
     1981 gcggtcacgc gacggcgggc ggcccggacg agatcatgct ggcctgcttc gacgatctgg
     2041 tcagcggcgt gggcgagggc accggttggc ggctgccggc ggtcgatgtg gcggcgctgg
     2101 gtggtcacac cggttcgctg gcgcaccacg caccgctgtg gggcggcaat ctggcgatgg
     2161 tggcgtcgct ggtcggcgcg ccctatttcc ctgaggtgaa gggcggtgtg ttgtttctgg
     2221 aagacgtcgc cgaacacccg tatcgcgtcg agcgcatgct ggcgcagctg ctgcacgccg
     2281 gcgtgctggc gcggcaaaag gccatcgtgc tcggccagtt caccgaatac aagctgacgc
     2341 cgcacgaccg cggctattcg atgaagaagg tcgtcgattg gctgcgcggg cagttgcccc
     2401 gcacgccggt gctcaccggc ctgccttttg gtcacgtgcc gaccaaggtg ttgctgccgg
     2461 tgggcgcgaa ggtcgattta ctggtggaag agcgcgacgc gctggtgctg tggggccaca
     2521 tgcactgatg tgatgcgaat gggcggcgct gccgcctggc gcgctatcag gtgcgcaacg
     2581 tggtcgggcg cgacatcatg cccttgggca gcgtgccctc gaacagcacc gaaaggctga
     2641 ccatgcgcgt gcgcgccacc tgctcgccgt gcaggccggc cagtgcgccc atcaggtcgg
     2701 cgcgcccata ccagagggcg agggcgtcgc cgccaaagcg gatgcggcgg gccacgtggg
     2761 ggtattgctg agcgccggcg tggcccagca tgtcaagcat cgccgtgcgg atgtcgtcca
     2821 cccggtgcgc cagacgcgcc tggtccggct cgtgccgaga caagcgcccg tacagtgcgc
     2881 tcaatccttc atgcttgaac catctcatct gtttgtcaca aataagttgc ggcctataac
     2941 cattggtaac catcataatc gggtacttat gcaaccggta ggcgcacgag tcgcgcacaa
     3001 gatgtttccg tgaaaaacag cgcatacatt gaaaaacacg cggtctgtcg tggcgcagcg
     3061 gccgggcggt gctacgattt ttgatggcaa agcgtgtagc agggatagcg ggacggcttg
     3121 ggcagcgtcc tgacgatgtg tcaacagaac aacaattcag cagtcaatga gttcgtcagt
     3181 caccatggtc ttcatcgacc tcgaaggcag caccgctgcc tatgccgcat tgggcaatgc
     3241 gcaggtggcc gacgtgatcg ccaaggtgac gcagtggatc ggccgggtgt gcgaagcgca
     3301 tggcgggcgc accgtcaagt ttctgggcga cggcgtactg gcgcagtttc cgcatggcgc
     3361 cgacgccgtg gaggccgccg tgttcatgca gctgaaccat tcggaacggc tggaaaagtg
     3421 gccggagccg ctgcgcatgg gcctcaagat cggcatggcc cggggcagcg tggtgcaaat
     3481 ggccgatgac gtctacggcg accccgtcaa cctggcggcc cgcctgtcag acatggcggg
     3541 cgcgcagacc atctgggccg atgaatcggt cgtcgtgcag ttgcgcgagg ggcgcgaacc
     3601 gtcttcgcgc gagacacacg tcgatggcga gcgcgcggca attgaaggtg cgcgctaccg
     3661 gtcgctgggc atgatgcggg tgcgcggcct gtcccagccg cactcggtgt tccagatcga
     3721 atggagcgag gatgtttcga ccgatctgat gacggtgcgc ggcgccttgc acgacatcga
     3781 agccagcgcc ggcccggtcg gcggcagcat cgcgctggcc tggctgggca attcgaaggt
     3841 ctacacggtg gacgatttgc cgttttccat cggccgcatc ccggacagcg gctttgtggt
     3901 cggtgaccag cgcgtttcac gcagccatgc ccgcatcgag ttcatcaacg gcgccttcgt
     3961 gctgagcgac ctcagcagct tcggcacctg ggttcggttt gccgacaacc cgggcgaggt
     4021 gcaactgcgc cgcaatcaat gcgtgctgca ttccaccggc gagatcgcgc tgggcgcgtc
     4081 cttcagcgat ttcagtgccg ccgtggtggc gttcagcgtc ggcgcgacgg aagcagcggg
     4141 ccgcggttcg tcgtcgacgc ggtcgctgtt caacgattag cggcagagtc ggcgaggtcg
     4201 gtggttcaag acgagacaag gattcatacg cttgattatt aacataatgc acatcgtatg
     4261 aagtaacgtc ggccttaaaa aagggcctga acccagcttc
//
LOCUS       DAOMVO010000211         4261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_5966_length_4261_cov_13.370899, whole genome shotgun sequence.
ACCESSION   DAOMVO010000211 DAOMVO010000000
VERSION     DAOMVO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_4261_cov_13.370899"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKV30_09980"
     CDS             <1..266
                     /locus_tag="PKV30_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRL30572.1"
                     /translation="KGQIAGKVLLVDDLADTGVTLQAVVERLKTGYAPITELRTAVIW
                     TKGCSSFAADYSVEDLPSNPWIHQPFEAYDSLTPEKLLAKWKV"
     gene            complement(218..799)
                     /locus_tag="PKV30_09985"
     CDS             complement(218..799)
                     /locus_tag="PKV30_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRL30573.1"
                     /translation="MKPGGWQMDSSVTAVSPDGASKDLGRHSMRVRLSAEPLAKEPYL
                     SPRIDDAKAPFLLVLSVRGARGAGAAGGGAGRIVVPWFAVGFVAVAGANSLHLLPGAW
                     QAGLLALDNLLLATAWPRWAWARDGRPSAAPAAGRWCWQPCCSAGWWQAARWSTAPSA
                     TGGTDRAARSAPGEEAAAQTFHLASSFSGVRLS"
     gene            964..2178
                     /locus_tag="PKV30_09990"
     CDS             964..2178
                     /locus_tag="PKV30_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL30574.1"
                     /translation="MIDPLTRLIHHPYTPPAGFAAPQPGVFKASTVIFPNVAAMRSRD
                     WKHKHGYTYGLHGTPTTFTLEERIATLEGGLQCVLVPSGLAAIANVALALLKTGDEVL
                     IPDNAYGPNKALAEGELAAFGITHQYYAPMDVADLAARTTPKTKLVWLEAAGSVTMEF
                     PDLIAQVRLCRERGVVSALDNTWGAGLAFNAFDLDGAGLGVDISAHALTKYPSGGGDV
                     LMGSVTTRDEALHLKIKLTHMRLGLGVGANDAEAVLRSLPTIGLRYRAQDQAARALAR
                     WCLTQPQLARVLHPALAGAPGHAHWQQVCGAADARGDGAAAGLFSVMVDARYTPAQVD
                     AFCDALKLFKLGYSWGGPVSLVVPYDLASMRGQWPAHLARGTLVRFSLGLEAVADLQA
                     DLTQALAQALPT"
     gene            2419..3165
                     /locus_tag="PKV30_09995"
     CDS             2419..3165
                     /locus_tag="PKV30_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRL30575.1"
                     /translation="MVPLGLSDPLRWLRLGWRDFLAAPGIGLFYGVCFWLMAVVLTLV
                     FRNKPEYVMSIASGCLLIGPFLAMGLYEVSRRREAGIKPELGASLTCWDKHVRSMGML
                     VLVLIVLELLWGRASLVVFAVFFNTGMPSTTGVLQAIFNPDNIEFVLVYLMVGGIFAL
                     LVYSTAVVSIPMILDRDTDAISAAITSIRVVFANPITMLWWGFLITLLIAIGLATWGV
                     GLIIIGPLLGHASWHAYRGAVRWQEPAAAA"
     gene            3211..3423
                     /locus_tag="PKV30_10000"
     CDS             3211..3423
                     /locus_tag="PKV30_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30576.1"
                     /translation="MATPNYGYEKRQRELEKKRKKEEKARAKAERKSGEGGDGPELDD
                     GATDAAEGSDGAGEGGGGGDGGGGGD"
     gene            complement(3498..>4261)
                     /gene="mnmH"
                     /locus_tag="PKV30_10005"
     CDS             complement(3498..>4261)
                     /gene="mnmH"
                     /locus_tag="PKV30_10005"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HRL30577.1"
                     /translation="GQIGFRVSLIEGGYKAFRAAMLADLPAQAARLCYRVVCGPTGSG
                     KTRLLHALRDAGAQVLDLEGLASHRASVLGLIPGQPQPSQKRFEMLVWEQLRGFDAAR
                     PVYVEAESKKVGNCTLPDALIDAMRASDCLRVELSHDERVALLLEDYPFFVSDPAFFC
                     QRLDTLIALRGHAVVDEWKRLVHAGEIEAVVRELLALHYDPGYATSTRRNFARFGEAL
                     PVALADRGPQALARAAEAIVQGQDDESAVSSAASA"
BASE COUNT          683 a         1470 c         1432 g          676 t
ORIGIN      
        1 ccaaggggca gattgccggc aaggtgctgc tggtcgatga cctggccgac accggcgtca
       61 ccctgcaggc ggtggtcgag cggctgaaaa ccggctacgc gcccatcacc gagttgcgca
      121 cggcggtcat ctggaccaag ggctgctcca gctttgcggc cgactattcg gtcgaagacc
      181 tgccctccaa cccgtggatt caccagccct tcgaggccta tgacagcctg acgcccgaga
      241 aactgctggc caagtggaag gtctgagcgg cagcctcttc acccggcgct gagcgcgcgg
      301 cgcggtcagt cccaccagtc gccgatggtg ccgttgacca gcgcgccgcc tgccaccagc
      361 cagccgaaca acacggttgc cagcaccatc ggccggccgc cggcgcggcg gatggccgac
      421 catcgcgtgc ccaggcccag cgcggccatg cggtggccag cagcagattg tccagcgcca
      481 gcaggcccgc ctgccaggcg ccgggcagca ggtgcagcga attcgccccg gccacggcga
      541 caaagcccac ggcgaaccac ggcacgacaa tgcggcccgc accgccgccc gccgcgccgg
      601 caccgcgtgc accgcgcacc gacagcacca gcaggaacgg cgccttggcg tcgtcgatgc
      661 gcgggctcag atagggctcc ttggccagcg gctcggccga caggcgcacc ctcatgctgt
      721 gccggcccag gtccttggat gcgccatcgg ggctgaccgc ggtgacgctg gagtccatct
      781 gccagccgcc gggtttcatc acgctctgcg catgcagcgg cgacgtcgcg agcgctgcac
      841 ataaggcatt agcggaaagg gcgatgcagg gcagggtggg catggttcga tgcttgcaaa
      901 gacggtcggg ccgatgatag tggccgcccg tggcgacccc ggcgctgcaa taccatagcc
      961 gccatgatcg atccactgac ccgcctgatc caccacccct acaccccgcc cgcaggattt
     1021 gccgcgccgc agccgggcgt gttcaaggcg tccaccgtca tcttccccaa cgtcgcggcg
     1081 atgcgctcgc gcgactggaa gcacaagcac ggctacacct acggcctgca cggcacgccg
     1141 accaccttca cgctggaaga gcgcatcgcc acgctggaag gtggcctgca gtgcgtgctg
     1201 gtgccgagcg gtctggcggc gattgccaac gtggcgctgg cgctgctgaa aaccggcgac
     1261 gaggtgctga tccccgacaa cgcctacggc cccaacaagg cgctggccga gggtgaactg
     1321 gcggctttcg gcatcacgca ccagtattac gccccaatgg acgtggccga cctggcggcg
     1381 cgcaccacgc cgaagacgaa actggtgtgg ctggaagccg ccggctcggt gacgatggaa
     1441 ttccccgacc tgatcgcgca ggtgcggctg tgccgcgagc gcggcgtggt cagcgcgctc
     1501 gacaacacct ggggcgccgg gctggccttc aacgcgtttg atctggatgg cgcgggcctt
     1561 ggcgtggata tttcggccca cgcgctgacc aagtacccca gcggcggcgg cgacgtgctg
     1621 atgggcagcg tgacgacgcg cgacgaggcg ctgcacctca agatcaagct cacccacatg
     1681 cggctcggcc tgggcgtggg cgccaacgat gctgaggccg tgctgcgctc gcttcccacc
     1741 atcggcctgc gctatcgcgc gcaagaccag gcggcgcggg cgctggcgcg ctggtgtctc
     1801 acgcagccgc aattggcgcg ggtgctgcat cccgccttgg ccggcgcacc cggccatgcg
     1861 cactggcagc aggtgtgcgg cgcggccgat gcgcgcggcg acggcgcggc ggcggggttg
     1921 ttcagcgtga tggtcgatgc acgctacacg ccggcgcagg tcgacgcctt ctgtgacgcg
     1981 ctcaagctgt tcaagctcgg ctacagctgg ggcggcccgg tcagcctggt ggtgccttac
     2041 gacctggcca gcatgcgcgg gcagtggcca gcgcatttgg cgcgcggcac gctggtgcgc
     2101 ttctcgctgg ggctggaagc ggtggccgat ctgcaggccg acctgaccca ggcccttgcg
     2161 caagcgttgc ccacttgact gcttcgtttt atatagctgc cagcgcttga gagacaagcg
     2221 ccggcggctg atttgattca aaatttcggc ctgctgcacc cagtggtgcc gcagcgcctg
     2281 tatcctgcgc cgcatccacc accttgccga ccgatgactg accgcgcctc cgacacgccc
     2341 cgcccggacg actcacccgc tgcgcccgcc gcctaccagc cctacgtgcc gccggtgccg
     2401 accgcgcccc aggccgtgat ggtgccgctc ggcctgtccg acccgctgcg ctggctgcgc
     2461 ttgggctggc gcgattttct ggccgcgccg ggcatcggct tgttctacgg cgtctgcttc
     2521 tggctcatgg cggtggtgct gacgctggtg ttccgcaaca agcccgaata cgtgatgtcg
     2581 atcgcctcgg gctgcctgct cattgggccg tttctggcca tgggcctgta tgaagtcagc
     2641 cgccggcgtg aggccggcat caagcccgag ctgggcgcct cgctcacctg ctgggacaag
     2701 cacgtccgca gcatgggcat gctggtgctg gtgctgatcg tgctggaact gctgtggggc
     2761 cgcgcctcgc tggtcgtgtt cgcggtgttc ttcaacaccg gcatgccgtc gaccaccggc
     2821 gtgctgcagg ccatcttcaa ccccgacaac atcgagttcg tgctggtcta cctgatggtg
     2881 ggcggcatct ttgcgctgct ggtgtattcg acggccgtgg tgtcgattcc gatgattctc
     2941 gatcgcgaca ccgacgccat cagcgccgcc atcaccagca tccgcgtggt gtttgcgaac
     3001 ccgatcacga tgctgtggtg gggctttctg atcacgctgc tgatcgccat cggcctggcc
     3061 acgtggggcg tggggttgat catcattggc ccgctcttgg gccatgccag ctggcatgcc
     3121 taccgcggcg ccgtgcgctg gcaagagcca gccgccgccg cgtgacgtgc tgggtgttac
     3181 agtggcccgc atcgatcagg aggcaacgca ttggcaaccc ccaactacgg ctacgaaaaa
     3241 cgccagcgcg agctggaaaa gaaacgcaag aaggaagaaa aagcccgcgc caaggctgag
     3301 cgcaagtcgg gcgagggcgg cgatggcccc gagttggacg atggcgccac cgatgcggcc
     3361 gaaggctcgg acggcgccgg tgagggtggc gggggtggcg acggcggtgg tggtggcgat
     3421 tgacgccatc cgctatcgat aagagagctg ctagcgctgg ctggtcaagc gccagcggct
     3481 gaaatcattc aaaaaactca agcgctggca gcgctgctga cggcgctttc gtcgtcctgc
     3541 ccttgaacga tggcctcggc cgcacgcgcc agcgcttgcg gtccgcggtc ggccagcgcc
     3601 accggcagtg cctcgccaaa gcgcgcgaag ttgcgccgcg tcgatgtggc gtaaccaggg
     3661 tcgtaatgca gcgccagcag ctcgcgcacc accgcttcga tctcgccagc gtgcaccagg
     3721 cgcttccatt cgtccaccac cgcgtggccg cgcagggcaa tgagcgtgtc gagccgctgg
     3781 cagaagaagg ccgggtcgct gacgaagaac gggtagtcct ccagcagcag cgccacgcgc
     3841 tcatcatgag acagctcgac gcgcaggcag tcgctggcgc gcatggcgtc gatcagcgcg
     3901 tcggggagcg tgcagttgcc gaccttcttg ctctcggcct ccacgtacac cgggcgcgcc
     3961 gcgtcaaagc cgcgcaattg ttcccacacc agcatctcga agcgcttttg cgagggctgc
     4021 ggctgccccg gaatcagccc cagcaccgac gcgcggtggc tggccagccc ttccagatcg
     4081 agcacctgcg cgcctgcatc acgcagcgcg tgcaacagtc gcgtcttgcc cgagccggtc
     4141 ggcccgcaca ccacgcggta gcagaggcgc gccgcctgcg ccggcaaatc ggccagcatg
     4201 gcggcgcgaa aggccttgta gccgccttcg atcaagctga cgcgaaagcc gatctgcccc
     4261 a
//
LOCUS       DAOMVO010000212         4221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6033_length_4221_cov_10.827172, whole genome shotgun sequence.
ACCESSION   DAOMVO010000212 DAOMVO010000000
VERSION     DAOMVO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6033_length_4221_cov_10.827172"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKV30_10010"
     CDS             complement(<1..386)
                     /locus_tag="PKV30_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL30578.1"
                     /translation="MSVQTIAAADALARLREFSAVIDARSEGEYAIDRLPGALNWPSL
                     HDDERVRVGTMYKQVNPFEARKLGAALVARNIASHIERECMDKPKHWRPLLYCWRGGQ
                     RSGALSLVLGQIGFRVSLIEGGYKAFR"
     gene            complement(383..997)
                     /locus_tag="PKV30_10015"
     CDS             complement(383..997)
                     /locus_tag="PKV30_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRL30579.1"
                     /translation="MLALAGGALSGAALAAEPPTILVVGDSLSAEYGIARGTGWVALL
                     EKKLAAEKIAAKVINASISGDTTAGGRSRLPALLKTHQPGVVVIELGSNDALRGLSLN
                     ATQANLADMTQAAQKAGARVLLVGMQMPPNYGADYGKRFFELYPKVAKAHNAALVPFF
                     LKGVADRANALELFQPDRIHPTAAAHPIILGNMWPEIQKLLKSK"
     gene            1043..1777
                     /locus_tag="PKV30_10020"
     CDS             1043..1777
                     /locus_tag="PKV30_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30580.1"
                     /translation="MTEPTPGTPPEALPPQIVAVEHVFKSVTDSTGTLDILRDIHFSL
                     AARETVAIVGASGSGKSTLLSIVAGLDTPTRGTVRVDGEDLFAVGEDARAALRARKIG
                     FVFQSFQLLGNLTALENVMLPLELAGRRDARQAASDMLARVGLSERLGHYPKVLSGGE
                     QQRVALARAFVVQPAVLLADEPTGSLDFATGEKVMELMFDLNREQGTTLVMVTHDRAI
                     AALCQRRLTVEAGRIVADERVDLLRS"
     gene            2283..3971
                     /locus_tag="PKV30_10025"
     CDS             2283..3971
                     /locus_tag="PKV30_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HRL30581.1"
                     /translation="MANERGKDLNEDPITGEPGSHPVGTGLGAAGGAAAGAALGTAVG
                     GPIGTVVGGIIGAVTGGLAGKDVAENLDPTAGGEPPEHKVATGVGATGGALTGAAMGS
                     IAGPLGTAAGAAIGAVAGGMAGRGVGEVVNPKAADELDEHNLASGTGAGAGALAGATV
                     GLAGGPVGAIVGAAIGSVAGAKAGEGVGEVVNPKAGDRLQDHELASGAGTGAGALAGA
                     AIGAVGGPVGAAVGAAVGAGLGSKAGKGLGEAVNPKAGDRVGEHNLATGTGAGAGAAT
                     GALVGAVGGPVGMAVGAAVGGLAGGALGHGAAEIVNPAAEDAYWRSAYAARPGYVRGY
                     DYDADYAGAYRLGYEGRGRYVGRGWDEVEPSLRSDWERAKDRSRLTWDEARYAVRDAW
                     DRVDGYNAAGAPRMRDTTAGMVESHPVGTTVGGVGGAATGAAIGAVGGPIGMAAGAVI
                     GGLAGAAAGHGVARAVNPTVEDAFWSENYVRAPGYVDGYTYDDYGPAYRLGYDGYGRY
                     AGRPYDDVETALEADWERIKGKSRLTWAEAKASTRAAWHRVETALPGDFDGDGR"
BASE COUNT          639 a         1398 c         1519 g          665 t
ORIGIN      
        1 cgaaaggcct tgtagccgcc ttcgatcaag ctgacgcgaa agccgatctg ccccagcacc
       61 agcgacagcg cgccgctgcg ttggccgccg cgccagcagt agagcagggg gcgccagtgc
      121 ttgggcttgt ccatgcactc gcgctcgatg tggctggcga tgttgcgcgc caccagcgcg
      181 gcgcccagct tgcgcgcctc gaacgggttc acttgcttgt acatcgtgcc cacgcgcacg
      241 cgctcgtcgt cgtgcaggct cggccagttc agggcgccgg gcaggcggtc gatggcgtat
      301 tcgccctcgc tgcgggcgtc gatgacggcg ctgaattcgc gcagccgcgc cagcgcgtcg
      361 gcggcggcga tggtttgaac gctcacttgg acttcaacaa cttctgaatc tcaggccaca
      421 tgttgcccag gatgatcggg tgcgcggcgg cggtggggtg aatgcggtcg ggctgaaaca
      481 gctccagcgc gttggcgcgg tcggccacgc ccttgaggaa aaacggcacc agcgccgcgt
      541 tgtgggcctt ggcgaccttt gggtacagct cgaaaaaccg cttgccgtaa tcggcgccgt
      601 agttgggcgg catctgcatg cccaccagca gcacccgggc gccggccttt tgcgcggcct
      661 gcgtcatgtc ggcgaggttg gcttgcgtgg cgttgagcga caggccgcgc agcgcgtcgt
      721 tgctgcccag ctcgatcacc accacgcccg gctggtgggt tttaagcagc gcgggcaggc
      781 gcgagcggcc gccggccgtg gtgtcgcccg agatgctggc gttgatcacc ttggcggcga
      841 ttttttcggc ggcgagtttt ttctccagca gcgcaaccca gccggtgccg cgggcgatgc
      901 cgtattcggc gctgagcgaa tcgccgacga ccaggatggt cggtggttcg gccgccagcg
      961 cggcgcccga caaagccccg ccagccaggg ccagcagggt ggagcggata aagtgtcgtc
     1021 ggtacaagaa acggcctttt caatgacaga acctacgccc ggcacaccgc cggaagcgct
     1081 ccctccccag atcgtcgcgg tggagcatgt tttcaagtcg gtcaccgatt ccaccggcac
     1141 gctggatatt ctgcgcgata tccatttcag cctggccgcg cgcgagaccg tggccatcgt
     1201 cggtgcctcg ggttcgggca agagcacgct gctgtccatc gtcgccgggc tcgacacgcc
     1261 cacgcgcggc acggtgcgcg tcgacggcga agacctgttt gccgtcggcg aagacgcccg
     1321 cgccgcgctg cgcgcacgca agatcggctt cgtgttccag agctttcaac tgctcggcaa
     1381 cctgaccgcg cttgaaaacg tgatgctgcc gctggagctg gccggccggc gcgacgcccg
     1441 ccaggcggcg agcgacatgc tggcgcgcgt cggcttgtct gagcgcctgg gccactatcc
     1501 caaggtgctg tcgggcggcg agcagcagcg cgtggcgctg gcacgcgcct tcgtcgtgca
     1561 gccggccgtg ctgctggccg acgagccaac cggcagcctg gatttcgcca ccggcgagaa
     1621 ggtgatggag ctgatgttcg acctcaaccg cgagcagggc accacgctgg tcatggtcac
     1681 gcacgaccgc gcgattgccg cgctgtgcca gcgccggctg accgtggagg ccgggcgcat
     1741 cgtggccgac gaacgggtcg atctgctccg atcttgatag ctgcttgcgc ttgactggca
     1801 agcgctgggg gctgagatga cgactggcga tggacgacaa cgccagaggc ggagatttcg
     1861 cagttggtct gttcttttac gtccttcgcg tccggtatct cgttttaaag cgcgtccaac
     1921 accaacgacc cggcaccatg gaggtgccag tttggtgaca tcacgatgac gtggcggccc
     1981 tgcgcgccca ctatgctgcg cgtccttgtc cgcgccggtg atcggcccac gttgctattc
     2041 aaccaatagc gcctcgcgct tgtcccgcca gcgccagcgg ccgtttttgc cgccacggcg
     2101 cgcaacggcg ccgcgtgctt gattgcgccg cgccattgct ttggcgcacg cgcgccgtcg
     2161 gcgcccgctg acaggccggg cacccggccg tgagcagact tgcgtcaccg atgcagcaca
     2221 cgctgaatcc atcggatgtg tcccccactt tgatgtcaca cacccattca ggagcattca
     2281 tcatggccaa cgaacgcggc aaggatttga acgaagaccc catcaccggc gagcccggtt
     2341 cgcacccggt cggcaccggc ctgggcgccg caggcggcgc ggccgccggc gcggctctgg
     2401 gcaccgccgt tggcgggccc atcggtacgg tggtgggcgg catcatcggt gccgtcaccg
     2461 gcggcctggc cggcaaggac gtggccgaga acctagaccc cacggccggc ggcgagccgc
     2521 ccgagcacaa ggtcgccacc ggcgtcggcg ccacgggcgg cgcgttgacc ggcgcggcca
     2581 tgggctccat cgccgggccg ctcggcaccg ccgctggcgc ggcaattggc gccgtggccg
     2641 gcggcatggc gggccggggc gttggcgaag tggtcaaccc caaggccgcc gacgaactgg
     2701 atgaacacaa cctggcttcc ggcaccggcg ccggtgccgg tgcactggcc ggcgccacgg
     2761 tcggcctggc aggcggcccg gtgggcgcca tcgttggcgc ggccatcggc agcgtggccg
     2821 gcgccaaggc gggtgaaggc gtgggcgaag tggtcaaccc caaggccggc gatcgcctgc
     2881 aggaccatga actggcgagc ggcgctggca ccggcgccgg cgcgctggcc ggcgcggcca
     2941 ttggcgccgt cggtggcccg gtcggcgcgg ccgtgggcgc cgcggtgggc gctgggctgg
     3001 gcagcaaggc cggcaagggc ttgggcgaag cggtcaaccc caaggcgggt gaccgcgtgg
     3061 gcgagcacaa cctggccacc ggcacaggtg ccggcgccgg ggcggctacg ggcgcgctgg
     3121 tcggggcagt gggcggcccg gtcggcatgg ctgtcggcgc ggctgtcggc ggcctggccg
     3181 gcggcgcact gggccatggc gcggccgaaa tcgtcaaccc cgccgccgaa gacgcctact
     3241 ggcgcagcgc ctacgccgcc cgccccggct acgtgcgggg ttatgactac gacgccgact
     3301 acgccggcgc ctatcgcctg ggctacgaag gccgtggccg ctatgttggt cgcggctggg
     3361 atgaggtcga gccttcgctg cgcagcgact gggaacgcgc caaggatcgc tcgcgcctga
     3421 cctgggacga agcccgctac gccgtgcgcg acgcctggga ccgcgtggac ggctacaacg
     3481 ccgccggcgc cccgcgcatg cgcgacacca cagcgggcat ggtcgaaagc cacccggtgg
     3541 gcaccaccgt gggcggcgtc ggcggtgcgg ccaccggtgc ggcgattggt gccgtgggcg
     3601 gcccgatcgg catggcagcc ggcgcggtga ttggcggcct ggcgggcgcg gcagccggcc
     3661 acggtgtggc gcgcgccgtc aacccgacgg ttgaagacgc cttctggagc gagaactatg
     3721 ttcgcgcgcc cggctatgtg gacggctaca cctacgacga ctacggcccg gcctaccgcc
     3781 tgggttacga cggttacggc cgctacgccg gtcgcccgta cgacgacgtc gagaccgcgc
     3841 tggaagccga ttgggagcgc atcaagggca agtcgcgcct gacgtgggcc gaagccaagg
     3901 cgtcgacccg cgccgcctgg caccgtgtgg agacggcgct gccgggcgac tttgacggcg
     3961 acgggcgttg agtgggcgat ggtcggcctg cggctgaccg tggttgagaa aacggcgctt
     4021 cggcgccgtt ttttttgcat tgatactatg gaaaagatag ctgtttgcgc tttctggaca
     4081 agcgctggag gttgttttct ttaatgggac catctgaggg tcgtcgaagg ccgaatacca
     4141 aaaaccgtac gcgagggacg caaaggattc gcgaaggacg caaaagaaaa cacgcaaaag
     4201 gtacttcttg aattttttgc g
//
LOCUS       DAOMVO010000213         4184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6099_length_4184_cov_12.896101, whole genome shotgun sequence.
ACCESSION   DAOMVO010000213 DAOMVO010000000
VERSION     DAOMVO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6099_length_4184_cov_12.896101"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..830)
                     /locus_tag="PKV30_10030"
     CDS             complement(12..830)
                     /locus_tag="PKV30_10030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL30582.1"
                     /translation="MNKSFLAASAATLLAGALALPALAQNVAIVNGKPVPKSRLEALE
                     AQIKAQAARTGQPVPPDVEKQLREEVIAREIFMQEAQRRGLEASPDFRAKMDLARQSV
                     LINELFADYQKNSAVTDAEAKAEYDRIVGSQAPAAGAKEYKARHILVEKEDEAKAIIA
                     QLKKGAKFEDIAKKQSKDPGSGAQGGDLGWANPSGYVPEFAQALGKLGKGQMTDAPVK
                     SQFGYHVIRVDDVREAKGPEVPSFEQVKPQVVQQLQQQKLVKFQEELRAKAKVE"
     gene            complement(1040..1312)
                     /locus_tag="PKV30_10035"
     CDS             complement(1040..1312)
                     /locus_tag="PKV30_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRL30583.1"
                     /translation="MSTALADRMAERLRAQLQPSALEVLDESWQHEGHAGANGTGFGT
                     HFRVRIASPLFEGKPRVARHRMVYDALQDFIDRDGVHALAIEATSP"
     gene            complement(1309..1869)
                     /locus_tag="PKV30_10040"
     CDS             complement(1309..1869)
                     /locus_tag="PKV30_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HRL30584.1"
                     /translation="MKLLLDFLPILLFFGAYKLYGIYVGTAVLMAATVVQMAIIYAID
                     KKLTTMHKATLALILVFGTLTLVLQDERFIKWKPTVLYTAMAIALAVALWGYGKNFLK
                     MMLGSQLALPDLVWNRLTVAWVLYCLFMAAINGYVAAYFSTDAWANFKLWGYVFPVAF
                     IIGQGIYVARHIQDEPAIDAPPGGST"
     gene            complement(1879..2286)
                     /gene="msrB"
                     /locus_tag="PKV30_10045"
     CDS             complement(1879..2286)
                     /gene="msrB"
                     /locus_tag="PKV30_10045"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRL30585.1"
                     /translation="MNHPVQKTDAEWRALLAEKGAEAPAFEVTRHAATERPFTGKYEQ
                     HWADGSYHCVCCGAKLFESGTKFDAGCGWPSFSLAIPGAIEERRDVSHGMVRVETVCA
                     SCGAHLGHVFPDGPQPTGLRYCMNSASLDFEPR"
     gene            complement(2283..3767)
                     /locus_tag="PKV30_10050"
     CDS             complement(2283..3767)
                     /locus_tag="PKV30_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HRL30586.1"
                     /translation="MQDTVTPVHPALDLGWRWRNAFEGLGGDFHSRLAPAALRDPYWI
                     ARSQSMARELGLQDDWWQAQEALEAFAGNRPLAGSAPLASVYSGHQFGVWAGQLGDGR
                     AHLLGEVQTPGGDWLELQLKGSGLTPYSRMGDGRAVLRSSIREFLCSEAMHGLGIPTT
                     RALCLTGSDQPVRREEIETAAVVTRVAPSFIRFGHFEHFSHHDQHDALKKLADFVIDH
                     HYPECRSAAQPYAALLAAVTRRTAALIAQWQAVGFMHGVMNTDNMSILGVTIDYGPFQ
                     FMDGFDPNHICNHSDHSGRYAYARQPQVAHWNLFALGQALLPLIGEPDDAMAALEPYK
                     TLFAAELDRRMAAKLGFAAPTDATRALTEELLTLMARERTDWTIFWRRLAHHAAGASA
                     DTVRELFLDRAAIDRVLLQYNELPALVGRAPGADLMLKSSPAIVLRNHLAEQAIALAK
                     QKDFRMVQDLLAVLEHPYDDYPDHADWAGFPPDWASHIQISCSS"
     gene            3850..>4184
                     /locus_tag="PKV30_10055"
     CDS             3850..>4184
                     /locus_tag="PKV30_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL30587.1"
                     /translation="MLGQMQNHPLLISSVIDFAARHQGDTEVVSRRVEGDIHRYTWAD
                     VQRRAKQVAKALDALGVKVGERVGTLAWNGYRHLELYFGVSGSGRVLHTVNPRLLPEQ
                     IAWIVNHAE"
BASE COUNT          683 a         1311 c         1449 g          741 t
ORIGIN      
        1 gcgccaggcg cttattcgac cttggccttg gcgcgcagct cttcctggaa cttcaccagc
       61 ttttgctgct gcaactgctg cacgacctgc ggcttgacct gctcgaaaga aggcacttca
      121 ggccccttgg cctcgcgcac gtcgtcgacg cgaatcacgt ggtaaccgaa ctgcgacttg
      181 accggcgcat cggtcatctg gcccttgccc agcttgccca gggcctgcgc gaattcaggc
      241 acgtagcccg acgggttggc ccagcccagg tcgccgccct gcgcgccgga gcccgggtct
      301 ttcgactgct tcttggcaat gtcctcgaac ttggcgcctt ttttcagctg ggcgatgatg
      361 gccttggcct cgtcttcctt ctcgaccagg atgtggcgcg ccttgtattc cttggcgccc
      421 gcggccggcg cctggctgcc gacaatgcgg tcgtattcgg ccttggcttc ggcgtcggtc
      481 acggcgctgt tcttctggta gtcggcaaac agctcgttga tcagcacgct ctggcgcgcc
      541 agatccatct tggcgcgaaa gtcgggcgag gcttccaggc cgcggcgctg cgcttcctgc
      601 atgaagattt cgcgcgcgat gacttcttcg cgcaactgct tttcgacgtc gggcggcacc
      661 ggctggccgg tgcgggcggc ctgcgccttg atctgcgcct caagcgcttc caggcgcgac
      721 ttgggcaccg gcttgccatt gacgatggcg acgttctgcg ccagggcggg cagcgccagg
      781 gcgccggcca gcagggtggc agcgctggcg gccagaaagg acttgttcat ggaggtcaaa
      841 acgttgaaaa accccagcgg ggcagaggat gccgcagcgc ggcgcaaagc agggattatc
      901 gcgtcttgga agggccagtg tctgcgacac agaacggccg cggagcaggc catgcgggtg
      961 gacgccgcgg acggacttgc gccggccgct ggcgttgtcc cccttcgcga agcaagaagg
     1021 gggaagccgc gtcagcggct cagggggatg tagcctcgat ggccagggca tgtacgccgt
     1081 cgcggtcgat gaagtcttgc agcgcgtcat agaccatccg gtggcgggcc acgcgcggct
     1141 tgccttcaaa caagggcgag gcaatgcgaa cccggaaatg cgtgccaaag cctgtgccat
     1201 tggcgccggc gtggccttcg tgctgccagc tttcgtccag cacctcgagc gcgctcggct
     1261 gcaactgcgc gcgcaggcgc tcggccatgc ggtcggccag ggcggtgctc atgtcgatcc
     1321 tccgggcggg gcgtcgatgg cgggttcgtc ctggatgtgg cgcgccacgt aaatgccctg
     1381 gccgatgatg aaggcgaccg ggaacacata gccccacagc ttgaagttgg cccaggcgtc
     1441 ggttgagaaa tacgccgcca cgtagccgtt gatggcggcc atgaaaaggc agtacagcac
     1501 ccaggccacc gtgaggcggt tccagaccaa gtccggcagc gcgagctggc tgcccagcat
     1561 catcttgaga aaattcttgc cgtagcccca cagcgccacc gccagcgcga tggccatggc
     1621 ggtgtagagc acggtgggct tccacttgat gaagcgttcg tcctgcagca cgagcgtgag
     1681 cgtgccgaac accaagatca gcgccagcgt ggccttgtgc atggtggtca gcttcttgtc
     1741 gatggcgtag atgatggcca tctgcaccac cgtggccgcc atcagcacgg cggtgccgac
     1801 gtagatgccg tacagcttgt aggcgccgaa gaacagcagg atgggcagga agtcgagcag
     1861 cagtttcatc ggggcggatt atcgcggctc gaaatcgagc gaggcggagt tcatgcaata
     1921 gcgtaggccg gtcggctgcg gcccatccgg aaacacatgc cccaggtgcg cgccgcaact
     1981 ggcgcacacc gtctcgacac gcaccatgcc gtggctgacg tcgcggcgct cctcgatggc
     2041 accgggaatc gccagcgaaa agctgggcca accgcagccg gcgtcgaact tggtgcccga
     2101 ttcaaacagc ttggcgccgc agcagacgca gtggtagctg ccgtcggccc agtgctgttc
     2161 gtacttgccg gtgaaggggc gctcggtggc ggcgtggcgg gtgacttcga aggcgggcgc
     2221 ttcggcgcct ttttcggcca gcagggcgcg ccactcggca tcggtttttt gtacagggtg
     2281 gttcatgagg agcaactgat ctggatgtgg gatgcccagt cgggcggaaa gccggcccaa
     2341 tcagcgtggt ccgggtaatc gtcgtaagga tgttccagca cggccagcag atcttggacc
     2401 atgcggaagt ccttttgttt cgccagggca atggcctgct cggccaggtg gttgcgcagc
     2461 acgatggcgg ggctggattt aagcatcaaa tcggcccctg gcgcacgtcc aacaagcgct
     2521 ggcagctcat tatattgtag caaaacacgg tcgatggcag cgcggtccag gaacagctcg
     2581 cgcaccgtgt cggcgctggc gcctgcagcg tggtgggcca ggcggcgcca gaagatggtc
     2641 cagtcggtgc gctcgcgcgc catgagcgtg agcagttcct cggtcagcgc acgggtggcg
     2701 tcggtgggcg cggcaaagcc cagcttggcg gccatgcggc ggtcgagttc ggccgcgaac
     2761 agggtcttgt acggctccag cgcggccatg gcgtcgtctg gctcgccgat cagcggcagc
     2821 agcgcctggc cgagggcgaa caggttccag tgcgccacct gcggctggcg ggcgtaggcg
     2881 tagcggcctg agtgatcgga atggttgcag atgtggttgg ggtcgaagcc gtccatgaac
     2941 tggaatgggc cgtagtcgat ggtcacaccc aggatgctca tgttgtcggt gttcatcacg
     3001 ccgtgcatga agcccaccgc ctgccactgc gcgatgaggg cggcggtgcg gcgcgtgacg
     3061 gcggccagca gcgcggcgta gggctgcgcg gcgctgcggc attcggggta gtggtggtcg
     3121 atgacgaaat cggccagttt tttcagcgcg tcgtgctggt cgtggtggct gaagtgctcg
     3181 aaatggccga agcggatgaa gctgggcgcc acgcgggtca ccacggcggc ggtttcgatc
     3241 tcttcgcgcc gcaccggctg gtctgagccg gtaaggcaca gcgcgcgcgt ggtcggaata
     3301 cccaggccgt gcatggcctc gctgcacaaa aattcgcgga tcgacgagcg cagcacggcg
     3361 cgcccgtcgc ccatgcgcga gtagggcgtg aggccgctgc ctttcagttg cagctcaagc
     3421 cagtcgccac cgggcgtctg cacctcgccc agcaggtgcg cgcggccatc gcccagttga
     3481 ccggcccaga cgccgaactg gtggccgctg tacacgctgg ccagcggcgc gctgccggcc
     3541 agcgggcggt tgccggcgaa tgcttccagc gcttcttgcg cctgccacca gtcgtcttgc
     3601 aagcccagct cgcgcgccat cgactggctg cgcgcgatcc agtacgggtc gcgcagcgcg
     3661 gcgggcgcca gccggctgtg aaagtcgcca cccaggccct cgaacgcgtt gcgccagcgc
     3721 cagcccaggt cgagggccgg atgaacgggg gtcacggtgt cttgcatggg ctcattttcc
     3781 cggctttgcg tccgtcgtgg attagtattc agatgaaccc tttcccccat aacttcaaga
     3841 ggagcgccaa tgctgggtca aatgcaaaac caccccctgt tgatttcgtc ggtgatcgac
     3901 ttcgccgcgc gccatcaggg cgataccgag gtcgtctcgc gccgcgtcga gggcgacatc
     3961 caccgctaca cctgggccga tgtgcagcgg cgtgccaagc aggtcgccaa ggcgctggac
     4021 gcgctggggg tcaaggtcgg cgagcgcgtg ggcacgctgg cctggaacgg ctaccgccac
     4081 ctggagctgt actttggcgt cagcggctcg ggccgcgtgc tgcacaccgt caacccgcgc
     4141 ctgctgcccg agcagatcgc ctggatcgtc aaccacgccg aaga
//
LOCUS       DAOMVO010000214         4163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6152_length_4163_cov_11.851753, whole genome shotgun sequence.
ACCESSION   DAOMVO010000214 DAOMVO010000000
VERSION     DAOMVO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6152_length_4163_cov_11.851753"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PKV30_10060"
     CDS             <1..1038
                     /locus_tag="PKV30_10060"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRL30588.1"
                     /translation="PKAQTVASGLQNPWAVAFLPDGRFLVTERPGRLRVVNADGQVQP
                     AVQGLPEVAAGGQGGLLDVITDSAFASNRTIYFCFSEPGKGTTNSTALARARLSQDAR
                     RLEDVKVIFSQQPKFSSTAHFGCRIVERPDGTLFLTLGDRFSRRDDAQTLDNHHGKVV
                     RVGKDGAIPKDNPFVGKAGALPEIWSYGHRNSQGATLAPDGSLWTHEHGARGGDEINR
                     PQAGKNYGWPQVSFGVNYDGSKIGSGDSAAPGLEPPLHHWTPSTAPSGMAFLTSDRYG
                     AGWKGNLFVGSLKFEYLDRIELKDGKVVAEHKLLEDLGQRIRDVRQGPDGLLYVLTDN
                     ANGRLIRLLHN"
     gene            complement(1102..2313)
                     /locus_tag="PKV30_10065"
     CDS             complement(1102..2313)
                     /locus_tag="PKV30_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRL30589.1"
                     /translation="METNRWPAARRAVHGAARAVGWIGVALALALGACGGSDDGTARL
                     SAEAALGEKMFHDVSLSASGRQSCASCHDARFGHGSPNGLAAQWGGPGLTLQGARVAP
                     SIRYLATAPAFHFDAEGTPTGGLFWDGRAASLADQAKGPLLNPVEMANASAAEVVAKL
                     ARADYAAEFQRLYGLDILQKPDEAFERIAQALARYQKEDPAFQPFSSKYDAVLRRQAS
                     LTPQEERGLALFKDEKKGNCAACHPADKAADGSHPLFTDFTYDNLGVPRNPELGHNAH
                     PAFFDLGLCARQGGDLAARTELCGAFKVPSLRNVALRQALFHNGHFKNLTDALRFYVQ
                     RDTHPEKWYPRRADGSIAVFNDLPPALHGNVNTAEVPYDRKPGDEPALTEAEIQDVIA
                     FLKTLTDGWKR"
     gene            complement(2377..3423)
                     /locus_tag="PKV30_10070"
     CDS             complement(2377..3423)
                     /locus_tag="PKV30_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL30590.1"
                     /translation="MKKQWVALAALALVWGAAAQAQEQVVNLYSARHYSTDEALYNNF
                     TKATGIKINRVDADDAGIIARLKAEGSASPADVILLVDAARLWRGEQDGLFLPIKSKL
                     LEDAIPANLRAPAADGGIPWFGFSTRARVVVYDKVRVKREDVDTYEELGDPKNKGKLC
                     IRSGAHPYNLSLFGAVTEHLGPEKSEAWLKGMVDNMARAPKGGDTDQIKAVAAGECQI
                     GVTNSYYLARMMRSNNPEDRAVVEKVGVVFPNQNSWGTHVNIAGAAVARHAKNQANAV
                     KFLEYLASPSAQNYFANGNNEWPVAKGVNTDNPALKSMTGGSGQFKSETIPISAVGAN
                     QVKVQQMLDRVGFK"
     gene            complement(3964..>4163)
                     /locus_tag="PKV30_10075"
     CDS             complement(3964..>4163)
                     /locus_tag="PKV30_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRL30591.1"
                     /translation="NAPAGSETAVAMMKSAVAAANNAFESVQKAVKQASDVAEANFNA
                     VTTSATTAAKNAGAAAKKAAR"
BASE COUNT          645 a         1341 c         1438 g          739 t
ORIGIN      
        1 cccaaggcgc agacggtggc gagcgggctg cagaacccct gggcggtcgc gtttctgccc
       61 gacgggcgct ttctggtcac cgagcggcca ggccgcctgc gcgtggtcaa cgccgacggc
      121 caggtgcagc ccgccgtgca gggcctgccc gaggtggcgg ccggcgggca gggcggtttg
      181 ctggatgtga tcaccgattc cgcgtttgcc agcaaccgca cgatctactt ttgcttcagc
      241 gagcccggca agggcacgac caacagcacg gcgctggcgc gtgcccggct gtctcaggat
      301 gcgaggcggc tggaggacgt gaaggtcatc ttcagccagc agcccaagtt cagcagcacg
      361 gcgcatttcg gttgccgcat cgtcgagcgg cccgacggca cgctgtttct cacgttgggt
      421 gaccgctttt cgcgccgcga cgacgcgcag acgctggaca accaccacgg caaggtggtt
      481 cgcgtgggca aggacggcgc gattccgaag gacaacccgt ttgtcggcaa ggccggcgcg
      541 ctgcccgaaa tctggagcta tggccaccgc aacagccagg gcgccacact ggcgcccgat
      601 ggcagcttgt ggacgcacga gcacggcgcg cgcggcggcg acgaaatcaa ccggccgcaa
      661 gccggcaaga actacggctg gccgcaggtg agctttggcg tcaactacga cggcagcaag
      721 atcggctcgg gcgacagcgc ggcgcccggg ctggagccgc cgctgcacca ctggacgccc
      781 tcgaccgcac cttcgggcat ggcgttcttg accagcgacc gctatggcgc cggttggaag
      841 ggcaacctgt tcgtcggctc actgaagttc gagtacctgg accgcatcga gctgaaagac
      901 ggcaaggtcg tggccgagca caagctgctt gaagacctgg gtcagcgcat tcgcgacgtg
      961 cgccaggggc cggacgggct gctctatgtg ctgaccgaca acgccaacgg caggctgatt
     1021 cgattgctac ataattgata gctacctgcg cttgcccatc aagcgctggc ggcccttttt
     1081 gtctgaatta tcgagggtgg gtcagcgttt ccagccgtcc gtcagcgtct tcaggaaagc
     1141 gatcacgtcc tgaatttccg cctcggtcag cgctggctcg tcgcccggct tgcggtcgta
     1201 gggcacttcg gcggtgttga cgttgccgtg cagcgcaggc ggcaggtcgt tgaacaccgc
     1261 gatactgccg tcggcgcggc gcggatacca cttctcgggg tgggtgtcgc gctgcacata
     1321 aaagcgcagg gcgtcggtca ggttcttgaa gtggccgttg tgaaacagcg cctgccgcag
     1381 cgccacgttg cgcaggctcg gcaccttgaa ggcgccgcac agttcggtgc gcgcggccag
     1441 atcgccgccc tggcgcgcgc acaggcccag atcgaagaag gccggatgcg cgttgtgccc
     1501 cagttcgggg ttgcgcggca cgcccaggtt gtcgtaggtg aagtcggtga acagcgggtg
     1561 gctgccgtcg gccgccttgt cggcggggtg gcaggcggcg cagttgcctt ttttctcgtc
     1621 cttgaacagc gccaggccgc gttcttcctg cggcgtcagg ctggcctgcc tgcgcagcac
     1681 ggcgtcgtac ttgctcgaga agggttgaaa cgccgggtct tccttctggt agcgagccag
     1741 tgcctgcgcg atgcgctcga acgcctcgtc gggcttttgc aggatgtcca gcccatagag
     1801 gcgctggaac tccgccgcgt agtcggcgcg ggccagcttc gccacgacct cggcggcgct
     1861 ggcgttggcc atctcgaccg ggttcagcag ggggcccttg gcctggtcgg ccagcgaggc
     1921 ggcgcggccg tcccagaaca ggccgccggt aggggtgcct tccgcgtcga aatggaacgc
     1981 gggcgccgtc gccagatagc ggatgctggg cgccacgcgc gcgccctgca gcgtcaggcc
     2041 ggggccgccc cactgggccg ccaggccgtt gggcgagccg tggccaaagc gcgcgtcgtg
     2101 gcaactggcg cacgactggc ggccggaggc cgacagcgag acgtcgtgga acattttctc
     2161 gcccagcgcg gcttcggcgg acaggcgggc ggtgccgtca tcgctgccgc cgcaggcccc
     2221 cagcgccagc gcgagcgcca ccccgatcca gccaacggcg cgcgcagcgc catgcacggc
     2281 ccgccgcgcg gcaggccacc gattcgtttc catgggtcac tcctccgggc gggcggcgcc
     2341 ttgtggccgg cgccgtgccg ccctccgtga tgcggattac ttgaagccga cgcggtccag
     2401 catctgctgc accttgacct ggttggcgcc gacggcgctg atcgggatcg tctcgctttt
     2461 gaactggcca ctgcccccgg tcatggactt gagcgccggg ttgtcggtgt tcacgccctt
     2521 ggccaccggc cactcgttgt tgccgttggc aaagtagttc tgcgccgacg ggctggccag
     2581 gtattcgagg aacttgaccg cgttggcctg gttcttggcg tggcgcgcca cagcggcgcc
     2641 ggcgatgttg acgtgcgtgc cccagctgtt ctggttgggg aacaccacgc ccaccttctc
     2701 gaccaccgcg cggtcttcgg ggttgttcga gcgcatcatg cgcgccaggt agtagctgtt
     2761 ggtgacgccg atctggcatt cgccggccgc cacggccttg atctggtcgg tgtcgccgcc
     2821 cttgggcgcg cgcgccatgt tgtcgaccat gcccttgagc caggcttcgg acttttcagg
     2881 ccccaggtgc tcggtcacgg cgccgaacag gctcaggttg taggggtgcg cgcccgagcg
     2941 gatgcacagt ttgcccttgt tcttggggtc gcccagttcc tcataggtgt cgacgtcctc
     3001 gcgcttgacg cgcaccttgt cgtacaccac cacgcgcgcg cgggtcgaga agccgaacca
     3061 cggaatgccg ccatcggccg ccggggcgcg caggttggcg gggatggcgt cttccagcag
     3121 cttggacttg atcggcagga acaggccgtc ctgctcgcca cgccacagcc gggccgcgtc
     3181 caccagcagg atcacgtcgg ccggcgaagc gctgccctcg gccttcaggc gcgcaatgat
     3241 gccggcgtcg tcggcgtcca cgcggttgat cttgatgccg gtggccttgg tgaagttgtt
     3301 gtacagcgcc tcatcggtgc tgtagtggcg cgccgaatac aggttgacaa cttgttcctg
     3361 cgcctgcgcc gcagcgcccc aaaccagcgc cagcgcggcg agcgcgaccc attgcttttt
     3421 catgctgtcc gttccttgat gaaaaccttg aacagcgaat cataacggta acgagaatgg
     3481 ttctcaatcg tgcaatttga tcgagcgcaa gccagcggcg aatgatgggc cggcgcttcg
     3541 accagctcag cgcgaatggc cttcaaatcc ggccggcgaa gcacggctgg cgcgaacggc
     3601 gaaaaaaggc aaggcgtcaa tccagtggcc gcggagcagg ccattccggg acaccgcgga
     3661 acgggcttgg cccggccgct ggtgtcgtcc cccgagggat ggcggccgca ggccgactca
     3721 gggggagcgt tctcgcgcaa aaaaagggcc gatcttgcga tcagcccttg aagggatccc
     3781 tgaacccgag ggtgtcgaaa ggatccgagg agacaaacgg ttggcgtctt gcccagtcgc
     3841 caaggcaaaa cctgcttggt gcaaatcagg ctttgctttg acgactttgc cgacacgggg
     3901 ccggcaaagt gcgttttcag cttatcggct caccggatgg acaggcgccc atccctggct
     3961 cgatcagcgg gctgcttttt tggcagcggc gccggcgttc ttggcagcgg tggtagcgct
     4021 ggtggtcaca gcgttgaagt tggcttcagc gacgtcagag gcttgcttga cagccttctg
     4081 caccgattca aacgcgttgt tggcggcagc cacggcgctc ttcatcatgg cgacggcggt
     4141 ctcggagcca gcgggggcgt tct
//
LOCUS       DAOMVO010000215         4140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6197_length_4140_cov_15.943696, whole genome shotgun sequence.
ACCESSION   DAOMVO010000215 DAOMVO010000000
VERSION     DAOMVO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6197_length_4140_cov_15.943696"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /gene="gloA"
                     /locus_tag="PKV30_10080"
     CDS             complement(<1..368)
                     /gene="gloA"
                     /locus_tag="PKV30_10080"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HRL30592.1"
                     /translation="MRILHTMLRVGDLQRSIDFYTQVLGMQLLRRSENPEYKYSLAFL
                     GFDGGNPGQAEIELTYNWGTTEYDTGNAYGHIAIGVPDAYAACDKIKAAGGNVTREAG
                     PVKGGSTVIAFVTDPDGYKI"
     gene            606..1154
                     /locus_tag="PKV30_10085"
     CDS             606..1154
                     /locus_tag="PKV30_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRL30593.1"
                     /translation="MTADHRMRSDPIGRLLHVLCDVCAVIGGAVLIAMAAMTVVSVIG
                     RTFFDSPILGDVELVQLGLAVCVATFLPYTQFRSANIIVDFFTTRASLRTQSWMDGLG
                     CLLYAGMTALIAWRVYAGGLMARDNQEASMLMNLPLWIPYMLMVPGLLLCTVVGLYQA
                     YKYWTTPAPAAVAPDATQEVAP"
     gene            1151..2473
                     /locus_tag="PKV30_10090"
     CDS             1151..2473
                     /locus_tag="PKV30_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRL30594.1"
                     /translation="MSTLTIGVLLFIFMLVLMAIRVPIAVAMFVPGVVGYVALSGWDP
                     LLAHLRGAVYGRVSVYDLSVIPLFMLMGGFAVNGGLSKALFDFANSLLGRFKGGMAMA
                     GILACAAFGAISGSTVATTATIAQVAYPEMRRIAYSGRLATAALATGGTMGVLLPPSV
                     TLMVYAILTEQNITKTFLAAYVPAILAALGYLVVIAIYVRLHPGHAPEARAASGAEVA
                     QAAAGVWPIALIFVAVFGGIYGGIFTPTEGAAIGATLTFIITLLQRKLTLQKAVESIR
                     TTAVTSGMIFMIFIGADMMNAALALSQLPNQLADLIAHAAIPPVVVMAVIMLFYVVLG
                     CVMDEMSMILLTVPVIFPVIMGLDFFGLEASDKALWFGILILTVCAIGMIFPPVGLNV
                     YVMNGLAKDVPMIETYKGVMIFLAWDFIRLALLILFPGITLWLVHLLT"
     gene            2504..3541
                     /locus_tag="PKV30_10095"
     CDS             2504..3541
                     /locus_tag="PKV30_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRL30595.1"
                     /translation="MASFSFKSAAAAAALTLAAGGAAQAETILKVHHFLPSGSSSHQK
                     IIAPWCAKVEKESGGELKCQIYPAMQLGGTPPQLLDQVRDGVADIVWSVPTYSAGRYT
                     KTEVFELPFMAVNAKQGSQALWEYTQKNSLDEFKGIKPLFLHTTEGYVLHSNKPITKL
                     EDMKGKKIRTATRISARMMSAMGGTPVQMPLPQVADALSKGVVDGVLVPWEAIPATKL
                     HEIVKYHLDAPLGSPRFANTIFIFGMNQAKYDSLSPKLKKVIDDNSGLAASAWAGEEG
                     FDKIVEPYSKLARDRGNTIAIISKDELARWEKATAGVDEIWLKDVAAKGGDGKKLIDE
                     AKALLQKYAPK"
     gene            complement(3667..>4140)
                     /locus_tag="PKV30_10100"
     CDS             complement(3667..>4140)
                     /locus_tag="PKV30_10100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRL30596.1"
                     /translation="GPGPGVARLDQVVGLTGLELMQAILNGELPYAAIAKTLDFVLVS
                     VSKGHATFQGTPGPQLLNPLGTVHGGWFATLLDSALGCAVHTMMPPGRSYTTAELGVN
                     LVKAITPKVQRVRAEGKVIHCGRQLATAEARLVGPDGTLYAHATTTCLVFDLPTT"
BASE COUNT          688 a         1349 c         1327 g          776 t
ORIGIN      
        1 tcgatcttgt agccgtccgg gtcggtgaca aaggcgatca cggtgctgcc gcccttcacc
       61 gggccggcct cgcgcgtcac gttgccgccg gcggccttga tcttgtcgca cgcggcgtac
      121 gcgtcgggca cgccgatggc gatgtggccg taggcgttgc ccgtgtcgta ttcggtggtg
      181 ccccagttgt aggtcagctc gatctcggcc tggccggggt tgccgccgtc aaagcccaga
      241 aaggcgagcg agtatttgta ctcggggttc tcggaccggc gcagcagctg catgccgagc
      301 acctgggtgt agaagtcgat cgagcgctgg aggtcgccca cgcgcagcat ggtgtgcagg
      361 attctcatgc ggccattatg gcctgccgcc gccgcccgct tgatgacagc cggcgcttgc
      421 catcatgacg cacatgaact atattgcaga ttagcaataa gcgaacgcgt taacgatggt
      481 tggttgacgg tgcggctagg ggaacatccc caagcggcgc cggcgccggc ttgattagac
      541 tcaagcaggc ggtaccggct gatcgacttt cgcgtcccgc cccacccgag gagagcaccg
      601 ctgccatgac agccgaccac cgaatgcgca gcgacccgat tggccgcctg ctgcacgtgc
      661 tgtgcgatgt gtgcgccgtc atcggcggcg cggtgctgat cgccatggcg gcgatgacgg
      721 tggtcagcgt catcggccgc accttctttg attcgccgat cctgggcgat gtcgagctgg
      781 tgcagctcgg cctggcggtg tgcgtggcca cctttctgcc ctacacgcag tttcgcagcg
      841 ccaacatcat cgtggacttc ttcaccaccc gggcttcgct gcgcacgcaa agctggatgg
      901 acggcctggg ctgcctgctg tacgccggca tgacggcgct gatcgcctgg cgcgtgtatg
      961 ccggcggcct gatggcgcgc gacaaccagg aagcatcgat gctgatgaac ctgccgctgt
     1021 ggattccgta catgctgatg gtgccgggcc tgctgctgtg caccgtggtc gggctgtacc
     1081 aggcctacaa gtactggacc acgcccgcgc ctgcagcggt ggcacccgac gccacgcaag
     1141 aggtcgcccc atgagcacct tgaccatcgg cgtgctgttg ttcatcttca tgctggtgct
     1201 gatggcgatt cgcgtgccga tcgcggtggc catgttcgtg ccgggcgtgg tcggctacgt
     1261 cgcgctttcc ggctgggatc cgctgctggc gcacctgcgc ggcgccgtgt acgggcgcgt
     1321 gtcggtgtac gacctgtcgg tgattccgct gttcatgctg atgggcggct ttgccgtcaa
     1381 cggcggcttg tcgaaggcgc tgttcgactt tgccaactcg ctgctcggcc gcttcaaggg
     1441 cggcatggcg atggcgggca tcctggcctg tgcggccttt ggcgccattt caggctcgac
     1501 cgtggccacc accgccacca tcgcgcaagt ggcttatccc gagatgcgcc gcatcgccta
     1561 ttcgggccgg ctggccaccg cggcgctggc caccggcggc accatgggcg tgctgctgcc
     1621 gccctcggtc acgctgatgg tgtacgccat cctgaccgag cagaacatca ccaagacctt
     1681 tctggccgcc tacgtgccgg ccattctggc ggcgctgggc tacctggtgg tgattgccat
     1741 ttacgtgcgc ctgcaccccg gccacgcgcc cgaagcgcgt gccgccagcg gcgccgaagt
     1801 ggcgcaggcg gctgccggcg tgtggcccat tgcactgatc ttcgtggcgg tgtttggcgg
     1861 catctacggc ggcatcttca cgccaaccga aggcgcggcc atcggcgcca cgctcacttt
     1921 catcatcacg ttgctgcagc gcaagctgac gctgcaaaag gcggtggaat cgatccgcac
     1981 cacggcggtc accagcggca tgatcttcat gatcttcatc ggcgccgaca tgatgaacgc
     2041 cgcgctggcg ctgtcgcagc tgcccaacca gctggccgac ttgatcgccc acgccgccat
     2101 tccgcccgtg gtggtgatgg ccgtcatcat gctgttctac gtggtgctgg gctgcgtgat
     2161 ggacgagatg tcgatgatcc tgctcaccgt gccggtgatt tttccggtca tcatggggct
     2221 cgactttttt ggtctggagg cgtccgacaa ggcgctgtgg ttcggcatcc tgatcctcac
     2281 cgtgtgcgcc atcggcatga tctttccgcc cgtcggcttg aacgtctatg tcatgaacgg
     2341 cctggccaag gacgtgccga tgatcgagac ctacaagggc gtcatgatct tcctggcctg
     2401 ggatttcatc cgcctggcgc tgctcatcct gttccccggc atcacgctgt ggctggtgca
     2461 tttgttgacc tgaaagctgt ttcaaacaag gagactcact cacatggcct cgttctcttt
     2521 caaatccgcc gccgctgccg cggcgctgac cctggccgcc ggtggcgcgg cgcaggccga
     2581 aaccatcctc aaggtgcacc actttttgcc ctcgggctcc agctcgcacc agaagatcat
     2641 cgcgccctgg tgcgccaagg tcgagaagga atcgggcggc gaactcaagt gccagatcta
     2701 ccccgccatg caactgggcg gcacgccgcc gcaactgctc gaccaggtgc gcgacggcgt
     2761 ggccgacatc gtgtggtcgg tgcccaccta ttcggccggc cgctacacca agacggaggt
     2821 gttcgagctg cccttcatgg ccgtcaacgc caagcagggc tcgcaggcac tgtgggaata
     2881 cacgcagaag aattcgctcg acgaattcaa gggcatcaag ccgctgttcc tgcacaccac
     2941 cgaaggttac gtgctgcact ccaacaagcc catcaccaag ctggaggaca tgaagggcaa
     3001 gaagatccgc accgccaccc gcatcagcgc ccgcatgatg tcggccatgg gcggcacgcc
     3061 ggtgcaaatg ccgctgccgc aggtggccga tgcgctgtcg aagggcgtgg tcgatggcgt
     3121 gctggtgccg tgggaagcca tccccgccac caagctgcac gagatcgtca aataccacct
     3181 ggatgcgccg ctcggctcgc cgcgctttgc caacaccatc ttcatcttcg gcatgaacca
     3241 ggccaagtac gacagcctgt cgccgaagtt gaagaaagtg atcgacgaca actcgggcct
     3301 ggccgcctcg gcctgggcgg gtgaagaagg cttcgacaag atcgtcgagc cctattccaa
     3361 gctggcgcgc gaccgcggca acaccattgc catcatcagc aaggacgagt tggcacgctg
     3421 ggaaaaagcc accgcgggcg tcgacgagat ctggctgaag gacgtggcgg ccaagggcgg
     3481 cgacggcaag aagctgatcg acgaagccaa ggcgctgctg caaaaatacg cgcccaagtg
     3541 atcggctggc cgaacggctg aacaacaaaa gccgctgcgg gtgcagcggc ttttttcatg
     3601 gctgtttagg cctccagcgc ttgtttgaca agcgcgagaa gctatcaaaa tagtattgcg
     3661 ccgcgctcag gtggtcggca aatcaaacac caggcaggtc gtggtggcat gcgcgtacag
     3721 cgtgccgtcc ggccccacca gccgcgcctc ggccgtggcc agctggcgcc cgcagtgaat
     3781 cactttgcct tcggcgcgca cgcgctgcac tttgggcgtg atggccttca ccaggttcac
     3841 gcccagctcg gccgtggtgt agctgcgccc cggcggcatc atggtgtgca cggcgcagcc
     3901 cagcgccgaa tcgagcagcg tggcaaacca gccgccgtgc accgtgccca gcgggttcag
     3961 caactgcggg cctggcgtgc cctggaaggt ggcgtgccct ttgctgacgc tcaccagcac
     4021 gaagtccagc gtcttggcga tggcggcgta gggcagctcg ccgttcagaa tcgcctgcat
     4081 cagttcaagg cccgtcagcc ccaccacctg atccagccgc gcgacaccgg ggccggggcc
//
LOCUS       DAOMVO010000216         4128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6213_length_4128_cov_11.195188, whole genome shotgun sequence.
ACCESSION   DAOMVO010000216 DAOMVO010000000
VERSION     DAOMVO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6213_length_4128_cov_11.195188"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKV30_10105"
     CDS             <1..279
                     /locus_tag="PKV30_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30597.1"
                     /translation="LAAQPLGKDRIIADPLEIARRAPDIIIGSWCGKKFRPEKVAARP
                     GWQDVPAVRDGQLFEIKSPDILQPGPAALTDGAEQLHRIVMDWMARHG"
     gene            272..862
                     /locus_tag="PKV30_10110"
     CDS             272..862
                     /locus_tag="PKV30_10110"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRL30598.1"
                     /translation="MVELTVARSELILGGQKSGKSRRAEQLAAAWLTHSPTHRALMIA
                     TAQAHDDEMRARIARHQRERAERVPGMTTLEAPRDLAAAIAQAGGAETLRVVDCLTLW
                     LTGQLMPLSNDFEQNRPPAHDWPAQAAMFLKATEACPGPVVLVGNEIGLGVIPLGREV
                     RAFVDALGVLNQQVAAACERVTLMAAGLPLCLKGAS"
     gene            1039..1902
                     /locus_tag="PKV30_10115"
     CDS             1039..1902
                     /locus_tag="PKV30_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30599.1"
                     /translation="MLAATAHAHQVTDDRGIPVHFDTPPQRVVSLLPSLTETVCAIGA
                     CARLIGVDRYSNFPPQVQKLPQVGGGLDPNIEAIVALKPDVVLMATSSRAADRLRALG
                     VKVVALETKTAADVRRVIGKLGQLLVVPDAERVAREIEAGVAAAAQSLPPALRGQRVY
                     FEASPGPHAAGPQSFIGEMLRALGLANIIGPELGPFPKINPELVVRADPDLIMAGERS
                     GSEIARRPGWASLRAVRGQRMCVFNAQEVDILVRPGPRMPEAARLMARCVMKTPPEPP
                     AASSPTGGQRQ"
     gene            2066..3094
                     /locus_tag="PKV30_10120"
     CDS             2066..3094
                     /locus_tag="PKV30_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRL30600.1"
                     /translation="MSTAQATLVQGAPAAPPSRTRVLTLVLLLLSLLAVLIGASVGAT
                     GFESVLRAAQDPVAWQIVTDIRLPRTLGAWLTGALLGLAGAVAQGLFRNPLADPFLLG
                     SASGAMLAVAIALAVLGAPVAAASWAAKLGLTGAAFVGALGGVLLTLLLAQGVQHTLR
                     LLLAGVVVGYVLGAVKDLVTLAAPDVLTGMLAFVLGNTGLLGWSACAVMAAVLLPMAL
                     LAWALARVLDGLALGEATAHTLGLPLAPMRAALIAVLALATGTAVAQAGLIGFVGLAA
                     PHLVRGMARVTHGPLVLLSAITGGLLLALADVVARVVIAPQELPVGVLTAVLGGSYLL
                     WLMYRRTR"
     gene            3106..3888
                     /locus_tag="PKV30_10125"
     CDS             3106..3888
                     /locus_tag="PKV30_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30601.1"
                     /translation="MNAPQKIAVSARSTGARIGKAQILHAIDVQIPVARWTAIVGPNG
                     AGKSTLLRVLAGLLPTDGVVQLHGKPLADWPARERARQLAWLGQGEAGAEDLIAYDVV
                     LLGRLPHRAWLAPPTAADHAAVQRALAEMQVAHLQSRRLGDLSGGERQRVLLARALAV
                     QAPVLLMDEPLAHLDPPHQADWLVVARAQVAAGGTVVSVLHELGLALHADDLLIVREG
                     RIVHHGPCSSPTTHRALQAAFDWRIGVYQVKDRWVALPRIPQ"
     gene            3899..>4128
                     /locus_tag="PKV30_10130"
     CDS             3899..>4128
                     /locus_tag="PKV30_10130"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRL30602.1"
                     /translation="MQIETPPTEKRYERPEGERRGLVIVNTGDGKGKSTAAFGLALRA
                     FGRQHVHGKQVKIFQFMKVPTARFGEHRMFEQ"
BASE COUNT          571 a         1438 c         1472 g          647 t
ORIGIN      
        1 ctcgcggccc agccgctggg caaggaccgc atcattgccg acccgctgga gatcgcccgc
       61 cgcgcgcccg acatcatcat tggctcgtgg tgcggcaaga agtttcggcc cgaaaaagtc
      121 gccgcgcgcc ccggctggca agacgtgccg gcggtgcgtg atggacaact gttcgagatc
      181 aagtcgcccg acatcctgca gcccggcccg gccgcgctga ccgatggggc cgagcaactg
      241 caccgcatcg tgatggactg gatggcgcgg catggttgaa ctgacggttg cgcgcagtga
      301 gttgattctg ggcggccaga aaagcggcaa gtcgcgccgc gccgaacaac tggccgccgc
      361 ctggctgacg cactcaccca cgcaccgcgc cttgatgatt gccaccgcgc aggcgcacga
      421 cgacgagatg cgcgcgcgca tcgcccgtca ccagcgcgag cgcgccgagc gcgtgccggg
      481 catgacgacg ctcgaagcgc cacgcgatct ggccgccgcc atcgcgcagg cgggcggtgc
      541 cgagacgctg cgcgtggtcg attgcctgac gctgtggctg accgggcagc tcatgccctt
      601 gtcaaacgat tttgagcaaa ataggcctcc agcgcacgac tggccagcgc aagcagctat
      661 gtttttaaaa gcaaccgaag catgccccgg ccctgtcgtg ctggtcggca acgagatcgg
      721 cctgggcgtg attccgcttg ggcgtgaagt gcgcgccttt gtcgatgcgc tgggtgtgct
      781 caatcaacaa gtggcggcgg cgtgcgagcg cgtcacgctg atggcggccg ggctgccgct
      841 gtgtttgaaa ggcgcgtcat gaatcggtgc tggcgaatca cgcgcacggc ttttgggtat
      901 ttctccctct ccctctggga gagggcaggg gtgagggcga cccacgctca atcttccacc
      961 gctgcgccct caccccagcc ctctcccgcg tgcggcagag ggagcaagtc gcgcgcctgg
     1021 tgttgggcgg ccgcgctcct gctggccgca acagcccacg cccaccaggt caccgacgac
     1081 cgcggcatcc ccgtgcactt cgacacgccg ccgcagcgcg tggtcagcct gctgccgtcg
     1141 ctgaccgaga ccgtgtgcgc catcggcgcc tgcgcgcggc tgatcggcgt cgatcgctat
     1201 tccaactttc cgccccaggt gcagaagctg ccccaggtcg gcggcgggct ggacccgaac
     1261 atcgaggcca tcgtcgcgct caaacccgac gtggtgctga tggcgacctc gtcgcgcgcg
     1321 gccgaccgcc tgcgcgcgct gggcgtgaag gtggtggcgc tggagaccaa gacggctgcc
     1381 gacgtgcggc gcgtgatcgg caagctgggc caactgctgg tcgtgcccga tgccgagcgc
     1441 gtggcgcgcg agatcgaagc cggcgtggcc gccgccgcgc aatccctgcc gcccgcgctg
     1501 cgcggccagc gcgtctactt cgaggccagc cccggcccgc acgcggcggg gccgcagtcc
     1561 ttcatcggcg agatgctgcg cgcactcggg ctggccaaca tcattggccc cgagctgggc
     1621 ccgttcccca agatcaatcc cgaactggtg gtgcgcgccg atcccgacct catcatggcc
     1681 ggcgagcgca gcggcagcga gatcgcgcgc cgcccgggct gggccagcct gcgcgccgtg
     1741 cgcgggcaac gcatgtgcgt gtttaacgcg caagaggtgg acatcctggt gcgccccggc
     1801 ccgcgcatgc ccgaggcggc gcggcttatg gcgcgctgcg tgatgaagac gccccctgag
     1861 ccgcctgcgg cgtcttcccc cactggggga caacgccagt gaccgggggg accccggcca
     1921 cggcgttccg gtatggcctg ctccgcggcc atcgggggtg gcctgctgcg cggccacagg
     1981 gacttggatt ggcacggcgt gtagtggacc aagaattttt ctccccctcc ctctgggaaa
     2041 gggcaggggt gagggcgacc cacgcatgag caccgcccaa gccaccctcg tgcagggcgc
     2101 ccccgccgcg ccgccctcgc gcacccgcgt gctgacgctg gtgctgctcc tgctctcgct
     2161 gctggcggtg ctgattggcg ccagcgttgg cgctaccggg tttgagagcg tgctgcgcgc
     2221 ggcgcaagac ccggtggctt ggcagatcgt gaccgacatc cgcctgccgc gcacgctggg
     2281 cgcctggctg acgggcgcgc tgctgggtct ggccggtgcg gtggcacagg ggctgtttcg
     2341 caacccgctg gccgatccgt tcttgctcgg cagcgcatcg ggcgcgatgc tggcggtggc
     2401 catcgccttg gctgtcctcg gcgcgccggt ggccgccgcg tcgtgggccg ccaagctggg
     2461 cctgaccggc gcggccttcg tcggcgcgct gggcggcgtg ctgctgacct tgctgctggc
     2521 gcaaggcgtg cagcacactc tgcgcctgct gctggccggc gtggtggtgg gctatgtgct
     2581 gggcgcggtg aaagacctgg ttacgctggc tgcgcccgat gtgctgaccg gcatgctggc
     2641 ctttgtgctg ggcaacaccg gtttgctcgg ctggagcgcg tgcgccgtga tggccgcggt
     2701 gctgctgccg atggcactgc tggcctgggc gctggcgcgc gtgctcgacg gcctggcctt
     2761 gggcgaagca accgcgcaca ccctgggcct gccgctggcg ccgatgcgcg cggcgctcat
     2821 cgccgtgctg gcgctggcca ccggcacggc ggtggcgcag gcgggcttga tcggctttgt
     2881 cggcctggct gcgccgcacc tggtgcgcgg catggcgcgc gtcacgcatg ggccgctggt
     2941 gctgctgagc gcgatcaccg gcggcctgct gctggcgctg gccgacgtgg tggcgcgcgt
     3001 cgtcatcgcg ccgcaggagc tgccggtggg cgtgctcaca gcggtgctgg gcggcagcta
     3061 tttgctgtgg ctgatgtatc ggcgcacgag atgactggcg tgcccatgaa tgctcctcaa
     3121 aaaatagctg tcagcgcacg atccacgggc gcccgcatcg gaaaagccca gatattgcac
     3181 gccatcgacg tgcagatccc ggtcgcgcgc tggaccgcca tcgtcggccc caacggcgct
     3241 ggcaaatcga cgctgctgcg cgtgctggcg gggctgctgc cgaccgatgg cgtggtgcaa
     3301 ctgcacggca agccgctggc cgactggccg gcgcgcgaac gggcgcgcca gctcgcctgg
     3361 ctggggcagg gcgaagcggg cgccgaagac ttgatcgcct acgacgtggt gctgctcggc
     3421 cgcctgccgc accgcgcctg gctggcgccg cccaccgccg ccgaccacgc cgccgtccag
     3481 cgcgccctgg ccgagatgca ggtcgcgcac ctgcaatcgc gccgcctggg cgatctgtcg
     3541 ggcggcgagc gccagcgcgt gctgctggcg cgcgccttgg ccgtgcaggc cccggtgctg
     3601 ctgatggacg agccgctggc ccacctcgat ccgccgcatc aggccgactg gctggtcgtg
     3661 gcgcgagccc aggtggccgc gggcggcacg gtggtcagcg tgctgcacga gttggggcta
     3721 gcgctgcatg ccgacgatct gctcatcgtc cgcgaaggcc gcatcgttca tcacggcccg
     3781 tgcagcagcc ccaccacgca ccgcgcgctg caagccgcgt tcgactggcg catcggcgtt
     3841 tatcaggtga aagaccgctg ggtggcgctg ccccgcattc cacagtaaca atccaaccat
     3901 gcagattgaa accccgccca ccgaaaagcg ctacgaacgc cccgagggcg agcgccgcgg
     3961 cctggtcatc gtcaacaccg gcgacggcaa gggtaagagc acggcagcct tcggcctggc
     4021 cctgcgcgcc tttggccgcc agcacgtgca cggcaaacag gtgaagattt ttcagttcat
     4081 gaaagtgccc acggcgcgct ttggcgagca ccgcatgttc gagcagat
//
LOCUS       DAOMVO010000217         4118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6233_length_4118_cov_13.044794, whole genome shotgun sequence.
ACCESSION   DAOMVO010000217 DAOMVO010000000
VERSION     DAOMVO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_4118_cov_13.044794"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKV30_10135"
     CDS             <1..617
                     /locus_tag="PKV30_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRL30603.1"
                     /translation="MKINPEKIRDVIGKGGATIRMLTEETGTTIDIGEDGTITIASTD
                     AAKADDAKRRIEAITAEAEIGKVYDGPITKLLEFGALVNILPGKDGLLHISQIAHERV
                     ERVADYLQEGQVVKVKVLETDDKGRIKLSMKALLDRPEGMEDRPPREFRERGDRPPRG
                     DRGDRGDRGDRGPREDRGDRPPRAEREERPAPAASAEGADQQQQ"
     gene            678..1691
                     /locus_tag="PKV30_10140"
     CDS             678..1691
                     /locus_tag="PKV30_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRL30604.1"
                     /translation="MKCIEIREYGAPEVLVPAERATPVAGAGEVLIRVSASGVNRPDV
                     LQRTGNYPVPPGASDLPGLEVAGVIESGDAAAMAQAGFKVGDRVCALVAGGGYAEYCV
                     APVAQCLPVPRGLTDVEAASLPETFFTVWSNVFDRGRLQKGETLLVQGGTSGIGVTAI
                     QLGKAAGATVIVTAGSDEKCAAALDLGADHAINYKATDFAEEAKKLTGGQGVDVILDM
                     VAGDYVKREVECLAEDGRLVIIAVQGGIKAEFNAGLVLRRRLTITGSTLRPRPVAFKG
                     AIAQALKKTVWPWLESGVVKPVIHSVFAATEAGDGLPSGAARAHALMQSGDHVGKIVL
                     TWS"
     gene            1694..2440
                     /gene="tpiA"
                     /locus_tag="PKV30_10145"
     CDS             1694..2440
                     /gene="tpiA"
                     /locus_tag="PKV30_10145"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRL30605.1"
                     /translation="MNQRKLIVGNWKMNGSLAANESLVRELLAGIGQPRCEVAVCPPG
                     IYLAQLQALLAGSPIELGVQNVSMHEAGAFTGDVSADMLRDFGVRYAIVGHSERRQHQ
                     AESDVHVAIKAKRALAAGITPIVCVGETLREREEGMTDFIVKRQLSAVIHLNGHCSSE
                     IVVAYEPVWAIGTGKTATPEQAQQVHAVLRAQIKAATEHADRVRILYGGSMNAANAAE
                     LLAQPDIDGGLIGGAALKAADFLKIVAAAA"
     gene            2479..2931
                     /gene="secG"
                     /locus_tag="PKV30_10150"
     CDS             2479..2931
                     /gene="secG"
                     /locus_tag="PKV30_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRL30606.1"
                     /translation="MNVVLNLVLVLQILSALAMIGLILVQHGKGADMGTAFGSGASGS
                     LFGASGSANFLSRSTAVLATVFLASTLALAYFGFSVRSAPAGGGGSVLDRAPISAPAS
                     GASAAVPAPAAGADAIPLGAAAVPAPSASAAAVPPAPAASGAAQIPTK"
     gene            3066..3150
                     /locus_tag="PKV30_10155"
     tRNA            3066..3150
                     /locus_tag="PKV30_10155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3100..3102,aa:Leu,seq:gag)
     gene            3209..3568
                     /locus_tag="PKV30_10160"
     CDS             3209..3568
                     /locus_tag="PKV30_10160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRL30607.1"
                     /translation="MNLAQYLPVLLFILVGLAVGALCLGMGAFLTPHKADAAKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVALKEVGAVGFAAVVIFLAI
                     LVVGFVYEWKKGALDWE"
     gene            3608..4087
                     /locus_tag="PKV30_10165"
     CDS             3608..4087
                     /locus_tag="PKV30_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRL30608.1"
                     /translation="MNDQLQQKGFLLTGVDAAVNWAKTGSLWPVTFGLACCAVEMMHA
                     AAARYDIARFGAEVFRASPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKVRRTNTIARV"
BASE COUNT          706 a         1310 c         1402 g          700 t
ORIGIN      
        1 cgatgaagat caaccccgag aagatccgcg acgtgatcgg caagggcggc gccacgatcc
       61 gcatgctgac ggaagaaacc ggcaccacca tcgacatcgg cgaagacggc accatcacca
      121 tcgccagcac cgacgccgcc aaggccgacg acgccaagcg ccgcatcgag gccatcacgg
      181 ccgaagccga gatcggcaag gtctacgacg gcccgatcac caagctgctg gagtttggcg
      241 cgctggtcaa catcctgccc ggcaaggacg gcctgctgca catcagccag atcgcgcacg
      301 agcgcgtgga gcgcgtggcc gactatctgc aagaaggcca ggtcgtcaag gtcaaggtgc
      361 tcgagaccga cgacaagggc cgcatcaagc tgtcgatgaa ggcgctgctg gatcgccccg
      421 aaggcatgga agaccgcccg ccgcgtgaat tccgtgagcg tggtgatcgc ccgccgcgtg
      481 gtgatcgtgg cgaccgcggt gaccgcggtg accgcggccc gcgcgaagac cgtggtgatc
      541 gcccgccgcg tgccgagcgt gaagagcgcc cggcgcccgc cgcgagcgcc gaaggcgccg
      601 atcagcaaca gcagtgattg ctgcgacttt gatagctgct cgcgcttgat gcacaagcgc
      661 cagaggcttg tttgaccatg aaatgcattg agatccgcga atacggtgcg cccgaggtgt
      721 tggtgcccgc cgagcgcgcc acgccggtgg ccggcgcggg cgaggtgctg atccgcgtca
      781 gcgcctccgg cgtcaaccgc cccgatgtgc tgcagcgcac tggtaactat cccgtgccgc
      841 ccggcgcgtc ggatctgccg gggctggaag tggcgggcgt catcgagtcg ggcgacgcgg
      901 cggccatggc gcaagcaggc ttcaaggtcg gcgaccgcgt gtgcgcgctg gtcgcgggcg
      961 gcggctacgc cgaatactgc gtagccccgg tcgcgcagtg cctgccggtg cccaggggtt
     1021 tgacggacgt cgaggcggcg tcgctgcccg agaccttctt caccgtgtgg agcaatgtgt
     1081 tcgaccgcgg tcgcctgcaa aagggcgaaa ccctgttggt gcagggcggc accagcggca
     1141 tcggcgtgac agcgattcaa ctcggcaagg cggcgggcgc gaccgtgatc gtgaccgctg
     1201 gttccgatga aaaatgcgcc gcagcgcttg atctgggtgc agaccatgct atcaattaca
     1261 aagcaaccga ctttgccgaa gaggcgaaga agctgaccgg gggccagggc gttgatgtca
     1321 tcctcgacat ggtcgccggc gactacgtca agcgcgaggt cgaatgtctg gctgaagacg
     1381 ggcgcctggt catcatcgcc gtgcaaggcg gcatcaaggc cgaattcaac gccggcctgg
     1441 tgctgcgccg tcgcttgacc atcacgggtt cgaccttgcg cccgcgcccg gtggcgttca
     1501 agggcgcgat tgcccaggcg ctgaaaaaaa ccgtgtggcc gtggctggag agcggcgttg
     1561 tcaagccggt gatccacagc gtgtttgccg ccaccgaagc gggcgacggc ctgcccagcg
     1621 gcgcggcgcg cgcgcatgcg ctgatgcaaa gcggcgacca cgtcggcaag atcgtgttga
     1681 cctggagttg agcatgaacc aacgcaaact catcgtcggc aactggaaga tgaacggctc
     1741 gctggcggcc aacgaatcgc tggtgcgcga gctgctcgcc ggcatcggcc agccgcgctg
     1801 cgaagtggcg gtgtgcccgc ccggcatcta cctggcccag ttgcaggcgt tgctggcggg
     1861 ttcgcccatc gagctgggcg tgcagaacgt gtcgatgcac gaggccggtg ccttcaccgg
     1921 cgacgtctcg gccgacatgc tgcgcgactt tggtgtgcgt tacgccatcg tcggccacag
     1981 cgagcggcgc cagcatcagg ccgagagcga cgtgcacgtg gccatcaagg ccaagcgcgc
     2041 gctggcggcg ggcatcacgc ccatcgtctg cgtcggcgaa acattgcgcg agcgcgaaga
     2101 gggcatgacc gatttcatcg tcaagcgcca gctgtcggcg gtgattcacc tcaacggtca
     2161 ttgcagcagc gagatcgtgg tcgcctacga acccgtgtgg gccatcggca ccggcaagac
     2221 ggccacgccc gaacaggcgc aacaggtcca cgccgtgctg cgcgcgcaga tcaaggccgc
     2281 gaccgaacac gcggatcgcg tgcgcatcct gtacggcggc agcatgaacg cggccaacgc
     2341 ggccgagctg ctggcacagc ccgacatcga tggcggcctg attggcggcg ccgccctcaa
     2401 ggccgcggat tttttgaaaa ttgtggccgc cgccgcttga tgcgcgtgcg cgggcagcta
     2461 tagattttgg agtattgaat gaacgtcgtc ttgaatctgg tgttggtgtt gcagatcctg
     2521 tctgcgttgg ccatgatcgg cctgatcctg gtgcagcacg gcaagggtgc cgacatgggc
     2581 acggcttttg gcagcggtgc ctcgggcagc ctgttcggtg cgtccggcag cgctaacttc
     2641 ctgtcgcgct ccacggccgt gctggccacg gtgtttctgg ccagcacgct ggccttggcc
     2701 tacttcggct tcagcgtgcg cagcgcgccg gcgggcggcg gcggcagcgt gctggatcgc
     2761 gcgcccatct cggccccggc cagcggcgct tcggccgccg tgccagcgcc cgcggcgggt
     2821 gctgatgcga tcccgttggg cgctgctgcg gtgcccgcgc catcggcttc ggccgccgct
     2881 gtgccgccgg caccggcagc cagcggcgcg gcgcagattc cgaccaagta agcgggctgt
     2941 cagcccgcac caaaaacgat tcttcccctc tgtaacgggt cgttttcagg gtaaactcct
     3001 aggtgcttcg gcgctgcgtc tgcagggtgt ttaagccccg cagaaagccc gatgcaagga
     3061 cacatgccgt cgtggtgaaa ttggtagaca cgctatcttg agggggtagt ggcgaaagct
     3121 gtgcgagttc gagtctcgcc gacggcacca agaacgcaag aacccggagg ccggcgatgc
     3181 ctccggggcg ccaaaacgaa gacacccgat gaatctcgcc cagtatcttc ccgttctgct
     3241 tttcatcctg gtcgggctgg ctgtcggggc gctctgtctg ggcatgggcg cgtttctcac
     3301 gccccacaaa gccgacgcgg ccaagaattc gccgtacgaa tgcggcttcg aggcgtttga
     3361 agacgcgcgc atgaagttcg acgtgcgcta ctacctcgtc gccatcctgt tcatcctgtt
     3421 cgaccttgaa atcgccttcc tgtttccgtg ggccgtggcg ctcaaggaag tgggcgctgt
     3481 cggctttgcg gcggtcgtca tttttctggc cattctggtg gtgggcttcg tctacgaatg
     3541 gaaaaaaggc gccctcgact gggaataagc cgcgcgtgac gtcttcgcgg cgacagggat
     3601 atctcacatg aatgatcagc ttcagcaaaa aggctttctg ctcaccggcg tcgatgcggc
     3661 ggtgaactgg gccaagaccg gctccttgtg gcccgtcacg ttcggcttgg cttgctgcgc
     3721 ggtggagatg atgcacgccg ccgcggcccg ctacgacatc gcgcgctttg gcgccgaggt
     3781 gtttcgcgcc agcccgcgcc agtccgacct gatgatcgtc gccggcacgc tgtgcaacaa
     3841 gatggcgccg gcgctgcgca aggtgtacga ccagatggcc gaaccgcgct gggtgatctc
     3901 gatgggctcg tgcgccaacg gcggcggtta ctaccactac agctattcgg tggtgcgcgg
     3961 ctgcgaccgc atcgtgccgg ttgacgtgta cgtgccgggc tgcccgccca cggccgaggc
     4021 gctgctgtac ggcatcatcc agttgcagaa caaggtgcgc cgcaccaaca ccatcgcgcg
     4081 ggtctgagca tggccgacat cgcaatttct cccgaggc
//
LOCUS       DAOMVO010000218         4094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6291_length_4094_cov_12.906660, whole genome shotgun sequence.
ACCESSION   DAOMVO010000218 DAOMVO010000000
VERSION     DAOMVO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6291_length_4094_cov_12.906660"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..>1525
                     /locus_tag="PKV30_10170"
     CDS             360..>1525
                     /locus_tag="PKV30_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HRL30609.1"
                     /translation="MSSPATPLDADACYRAVAAHDARFDGAFFIGVTSTGIYCRPVCR
                     VKTPRRENCRFFAHASQAEGAGFRPCLRCRPELAPQTLAWSAQDASALLAQHAARLLD
                     SPDAWGDEVPSVAGLAARLGVSDRHLRRVFEAELGVSPMDWLQTRRLLAAKQLLADTR
                     LPVAQVALAAGFASLRRFNAAFAAQYRMSPTALRRDGSEPAPRDGIAVTLGFRPPYDV
                     REMLQFLQQRGIAGVEQIDQSTHTVARTLRLDDDTRGWIACRFEPEQHRVQVRVADSL
                     APQLPRVIARVRAWLDLDADPAAIHAVLGADFPALAGLRVPGTLDGFELAVRAVLGQQ
                     VTVAAARTLTTRLVERFGTPLATPIDGLTRLFPSPAALAAASGDELGQLGLVRQR"
     assembly_gap    1526..1625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1734..2303
                     /locus_tag="PKV30_10175"
     CDS             1734..2303
                     /locus_tag="PKV30_10175"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRL30610.1"
                     /translation="MIKHLHTVHTLTATPLGHLLLAATPQGLSGAWFIEGQRGLPDWS
                     SWGAPTHGHPLLGQAAREMDEYFAGERQRFDVPLDLSGGTPFQQSVWHALLGIDAGDS
                     QSYGQVAARIGHPAAVRAVGTAVGANPVSVIVPCHRVLGSTGTLAGYGGGLGRKVALL
                     LREGWQVEAPAAGAEPAPTLRPQRVAVRR"
     gene            2376..3263
                     /locus_tag="PKV30_10180"
     CDS             2376..3263
                     /locus_tag="PKV30_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL30611.1"
                     /translation="MSAAVAAEFILLAALWGASFLFMRLGTLEFGPLPTAGLRVAIAS
                     LALLPVMLSRGLWPQLRQHWKPVMICGLINSAIPFALFSFALLSISTGLSSILNATVP
                     LFGALVAWLWLGDKPGTSRTVGLVIGFIGVALLASGKASFKPDASGAVTAWGILACLL
                     ATISYAVAASFTRRYLSGLNSLMVATGSQIGAALGLALPTLLMWPVQAPSLKAWGALL
                     ALGILCTAVAYVLFFRLIEQLGPARAITVTFTIPVFAVFYGVTLLGETVTTWMLFCGV
                     IVLCGTALATGLVKLPLDR"
     gene            complement(3268..3912)
                     /locus_tag="PKV30_10185"
     CDS             complement(3268..3912)
                     /locus_tag="PKV30_10185"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRL30612.1"
                     /translation="MFTGIITAVGRIAEVRDLGASSAHGKQLVIEAPAGYLQDVGLGD
                     SIALNGACMTATQIDAEAGRFAIDISAESLARTAGLDRVGPVNLEKALRAHDRLGGHI
                     VSGHVDGMGRVTHFAPVGESFELRILAPRELAKYLAYKGSITVNGVSLTVNTVTDQAE
                     GCEISINLIPHTVQNTALHALTAGAQVNLEIDLIARYVERMLSAPASQAPAAIK"
     gene            complement(3958..4034)
                     /locus_tag="PKV30_10190"
     tRNA            complement(3958..4034)
                     /locus_tag="PKV30_10190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3998..4000),aa:Arg,seq:acg)
BASE COUNT          558 a         1385 c         1330 g          721 t
ORIGIN      
        1 tcgcccagcg ccatcgcctg cgtgccgaaa gccagcccga tggcctcggc caccggctcg
       61 ttcagcgtgg tcggcgtggt gccgcgaatg tcgtaggcct tgaagatgct gggggtgact
      121 tgcatgggcg gagattgtag ggagcgctct ggatggcaaa ggccgacaaa cagccgtacg
      181 cgaaggacgc aaaggattcg cgaaggacgc gaaagaaaac cttttttgaa tttgtctttt
      241 ttcgcgtcct tcgcgtgatc ttcgcgccct tcgcgtccgg ctgttgattc aggcgtctcc
      301 aaaatcacca tgtccgaaaa cggcgagttt tcgcgtcgcc gaaacctatc attgcggcca
      361 tgtcctcgcc cgccaccccc ctcgacgccg acgcctgcta ccgcgccgtg gctgcgcacg
      421 atgcgcgttt tgacggcgcg tttttcattg gcgtgacctc caccggcatc tattgccgcc
      481 cggtctgccg cgtgaagaca ccgcgacgcg agaactgtcg cttctttgcc cacgcctcgc
      541 aggccgaagg cgccggcttt cgaccctgcc tgcgctgccg gcccgagctg gctccgcaga
      601 cgctggcctg gtcggcgcaa gacgcgtccg ccctgctggc ccagcacgcg gcgcgcctgc
      661 tggattcgcc cgacgcctgg ggcgatgagg tgcccagcgt ggccgggctg gccgcgcgcc
      721 tgggcgtgag cgaccgccac ctgcgtcgcg tgttcgaggc cgagttgggc gtgtcgccca
      781 tggactggct gcaaacgcgc cgcctgctcg ccgccaagca gttgctggcc gacacgcggc
      841 tgcccgtcgc gcaggtggcg ctggcggccg gttttgccag cctgcgtcgc ttcaacgccg
      901 cgtttgccgc gcagtaccgc atgagcccga cggcgttgcg gcgtgacggc agcgagccgg
      961 caccgcgcga cggcattgcg gtcactttag gcttccgacc cccgtacgac gttcgcgaga
     1021 tgctgcaatt tttgcagcaa cgtggcatcg cgggcgtgga acagattgac caaagcaccc
     1081 acaccgtggc ccgtacgctg cggctggacg acgacacccg cggctggatc gcctgccgct
     1141 tcgagcccga gcagcaccgc gtgcaggtgc gcgtggccga ctcgctggcg ccgcagctgc
     1201 cgcgcgtgat cgcccgcgtg cgcgcctggc tcgacctgga tgccgaccct gccgccatcc
     1261 acgccgtgct gggcgccgac ttccccgcgc tggcgggtct gcgcgtgccg ggcacgctgg
     1321 acggttttga gctggcggtg cgcgccgtcc ttggccagca ggtcacggtg gcggcggcgc
     1381 gcacgctgac gacgcgactg gtcgagcgct ttggcacgcc gctggccacg cccatcgacg
     1441 gactcacgcg gctgttccct agccccgccg cgctggccgc cgccagcggc gacgaactgg
     1501 gccagctggg tctggtgcgc cagcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnccctg gcgcgcctac gccgtgatac gggcgtggca ttcgccactc cattcactat
     1681 caactcaata gctgctcacg caatacagac aagcgccaaa ggggattttc atcatgatca
     1741 agcatcttca caccgttcac accctgaccg ccacgccgct cggccacttg ctgctggccg
     1801 ccacgccgca aggcctgtcg ggcgcgtggt tcatcgaagg ccagcgcggc ctgcccgact
     1861 ggtcgagctg gggcgcgccg acgcacggcc acccgctgct cggccaggcc gcgcgcgaaa
     1921 tggacgagta cttcgccggc gagcgccagc gctttgatgt gccacttgat ctgtcgggcg
     1981 gtacgccgtt tcagcaaagc gtgtggcacg cgttgctggg catcgacgcc ggtgattcgc
     2041 aaagctacgg ccaggtggcg gcgcgcatcg gccacccggc tgccgtgcgc gccgtcggca
     2101 ccgccgtcgg cgccaacccg gtcagcgtga tcgtgccctg ccaccgcgtg ctgggcagca
     2161 ccggcacgct ggccggctac ggcgggggcc tggggcgaaa ggtggcgctg ctgctgcgag
     2221 agggctggca ggtggaggcg ccggccgccg gcgccgagcc cgcgcccacc ctgcgcccgc
     2281 agcgcgtcgc cgtgcggcgt tgatgtcacg ggcgcatcaa tcagacgttc atccagcatc
     2341 acgccgccga tcgcgcggcc ccatactcca cgcccatgtc tgccgccgtc gccgccgaat
     2401 tcatcttgct ggccgccctt tggggcgcgt cgtttttgtt catgcggctc ggcacgctgg
     2461 aattcggccc gctgcccacg gcaggactgc gcgtggcgat tgcctcgctc gcgctgctgc
     2521 cggtcatgct gtcgcgcggg ctgtggccgc agctgcgcca gcactggaag ccggtgatga
     2581 tctgcggcct gatcaactcg gcgatcccgt ttgcgctgtt cagctttgcg ctgctgtcga
     2641 tcagcaccgg gctgtcgtcg atcctgaacg ccaccgtgcc gctgttcggc gcgctggtgg
     2701 cgtggctgtg gctgggtgac aagcccggca cctcgcgcac ggtgggcctg gtcatcggct
     2761 tcatcggcgt cgcgctgctg gccagcggca aggccagctt caagcccgac gcgtcgggtg
     2821 ccgtcaccgc ctggggcatc ctggcctgcc tgctggccac catcagttat gccgtggcgg
     2881 ccagcttcac gcggcgctat ctgtcgggtt tgaattcgct gatggtcgcc accggcagcc
     2941 agatcggcgc ggcgctcggt ctggcgctgc ccacgctgct gatgtggccc gtacaggcgc
     3001 cgagcctgaa agcctggggc gcgctgctgg cgctgggcat tctgtgcacc gccgtggcct
     3061 acgtgctgtt tttccggctc atcgagcagc tgggaccggc gcgcgccatc accgtcactt
     3121 tcaccatccc ggtatttgcc gtgttctacg gcgtcacgtt gctgggcgaa acggtgacga
     3181 cgtggatgct gttctgcggc gtgatcgtgc tgtgcggcac ggcgctggcg acggggctgg
     3241 tcaagctgcc gctcgaccgc tgactatcta tttgatagct gctggcgctt ggctggcggg
     3301 cgctgagagc attctttcga cataacgggc gatcaggtcg atctccagat tcacctgcgc
     3361 gccagcggtc agcgcatgca gcgccgtgtt ctgcaccgtg tgcggaatca ggttgatgct
     3421 gatctcgcag ccctccgctt ggtcggtcac ggtattgacc gtcaggctca cgccgttcac
     3481 ggtgatcgag cccttgtagg ccaggtactt ggccagctcg cgcggcgcca ggatgcgcag
     3541 ctcaaagctc tcgcccaccg gcgcgaagtg cgtcacgcga cccatgccgt cgacgtggcc
     3601 cgagacgatg tggccgccca gccggtcgtg cgcgcgcagg gctttttcca gattcaccgg
     3661 ccccacgcga tccagcccgg cggtgcgcgc cagcgattcg gccgaaatgt cgatggcaaa
     3721 gcgcccggcc tccgcgtcga tctgcgtcgc cgtcatgcag gcgccgttga gcgcgatgct
     3781 gtcgccgagt cccacgtcct gcagatagcc ggccggcgct tcgatgacca gttgcttgcc
     3841 gtgcgcgctg ctcgcgccca gatcgcgcac ctcggcgatg cggccgacgg cggtgatgat
     3901 gcctgtgaac atgaaaaaac cccgcgactc tttcaagtcg cggggttttc aaagagatgg
     3961 cgcggctggc aggattcgaa cccacgaccc cttggttcgt agccaagtac tctatccaac
     4021 tgagctacag ccgcgcgaag cccgcaagta tatcaccgaa atctggtagg ccgtgcaggg
     4081 ctcgaacctg cgac
//
LOCUS       DAOMVO010000219         4077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6328_length_4077_cov_15.838140, whole genome shotgun sequence.
ACCESSION   DAOMVO010000219 DAOMVO010000000
VERSION     DAOMVO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6328_length_4077_cov_15.838140"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /gene="argS"
                     /locus_tag="PKV30_10195"
     CDS             complement(<1..170)
                     /gene="argS"
                     /locus_tag="PKV30_10195"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655403.1"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRL30613.1"
                     /translation="MQSVKNELLAALAAELEKIAPGAGDKAAFESPKQAAHGDLAVTA
                     AMQLAKPLKSNPR"
     gene            260..3040
                     /gene="ppc"
                     /locus_tag="PKV30_10200"
     CDS             260..3040
                     /gene="ppc"
                     /locus_tag="PKV30_10200"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRL30614.1"
                     /translation="MAASSPHATTDTPLDKDTPLRDDIRLLGRLLGDTLRQQEGQALY
                     ELVEQVRRTAIQFARHNHPEARAGMAALLDPLPQAQAQVVVRAFSYFLQLANIAEDEH
                     HLRRRRAHDLADAPPREGSLAHALGRLAADGVSAEALADVLGHALVAPVLTAHPTEVQ
                     RQSLIHNHRLIARLLDERERQRLTPEEAADNDTCLTDAVLRMWQTRMLRPVRLNVIDE
                     VRNGISYFEETFFTELPRLYLQAERQLRQHFPGQPWQLPPLMRVGSWIGGDRDGNPFV
                     TAETLRQAMRLQSSTALARYQAEAHALGSELALSELLVRPTPALLALAERAADPSPQR
                     ADEPYRRALIGIYARLAATHRQLDPSAPPQHGVADAAPYAGPDALGADLAVLSESLRS
                     HGSAALANGRLARLQRAVQVFGFHLAPIDLRQNSDVHVRTVAELLAQAGRCADYAALP
                     EDARIALLGEELASTRPLRSSYLDYSEETRAELAIFDAAREIRQQHGSAALVNCIISK
                     ADGVSDMLELALLLKESGLLRAGPEPRTDVNLIPLFETIDDLQRSAAIMDALFRLPGY
                     RSLVATRGDEQEVMLGYSDSNKDGGFLTSGWELYKAEIALVQVCQAHGVRLRLFHGRG
                     GSVGRGGGPSYHAILAQPPGAVQGQIRLTEQGEVISAKYGTPETGRRNLEVLLAATLE
                     ASLAQQAGEAAAAQRFQPVMDALSQRAFAAYRALVYETPGFATYFQQSTVVSEIAALN
                     IGSRPASRKPSQRIEDLRAIPWVFSWSQCRLMLPGWYGFGSAVEGYLQAHPEGLASLR
                     EMGRTWSFFASLLSNMDMVLAKTDLAIASRYADLVTDVALRSRIFERISAEWALTRRH
                     LLAILEQDELLADNRTLRQSLQLRSPYMDPLNHLQVELLKRYRAGDTSARLAAGIHIT
                     INGIASGLRNSG"
     gene            complement(3116..3565)
                     /locus_tag="PKV30_10205"
     CDS             complement(3116..3565)
                     /locus_tag="PKV30_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HRL30615.1"
                     /translation="MLEPWLAFFHISAVLGWIVFASSEAALCRREWLNAAVVQRLARL
                     DRILWIATAAVLLTGLARIYLGAKGAAWYWGNWLLHLKLTLFFITALLMIGPTRRIAR
                     WQARLAAGGSLPPEAEISGVRKLVMIATHLVALIPLAAVFLARGFGA"
     gene            complement(3650..>4077)
                     /locus_tag="PKV30_10210"
     CDS             complement(3650..>4077)
                     /locus_tag="PKV30_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL30616.1"
                     /translation="RNAVGRNDERTPKGAGVPRHAVGRDDERTLAGAGAPNEAESELT
                     YLRPGGPLDCSDGQVLHDWCLAGHGIAWRSTWEVEGEIAAGLLVPVLEEFAAPPNGIY
                     AILPHATRHLPLRVRLWVDFLRQRYGDPAFWRAAAQADS"
BASE COUNT          624 a         1499 c         1372 g          582 t
ORIGIN      
        1 cgcgggttgc ttttgagcgg cttggccagt tgcatggcgg cggtcaccgc caggtcgccg
       61 tgcgcggcct gcttgggcga ttcaaacgcg gccttgtcgc ccgcgccggg cgcgattttt
      121 tccagctcgg ccgccagcgc ggcaagcagt tcgttcttca cggattgcat cgacggattc
      181 taggcgggtg gcggtttgcc acccgcgccc gatcggcgat gatcgtcacc accccaccac
      241 gcccgagctg cacgcaccca tggccgcttc ttcgccgcac gccaccaccg acacgccgct
      301 cgacaaggac accccgctgc gcgacgacat ccgcctgctt ggccgcctgt tgggcgacac
      361 gctgcgccag caggaaggcc aggccttgta cgagctggtc gagcaggtgc ggcgcaccgc
      421 catccagttt gcgcggcaca accaccccga agcgcgcgcc ggcatggccg cgctgctcga
      481 tccgctgccg caggcgcagg cccaggtggt ggtgcgcgcc ttcagctact tcctgcagct
      541 ggccaacatt gccgaggacg agcaccacct gcgccgccgg cgcgcccacg acctggccga
      601 cgcgccaccg cgcgaaggca gcctggccca tgcgctgggc cgcctggcgg ccgatggcgt
      661 gagcgccgaa gcgctggcgg acgtgctggg ccacgcgctg gtcgccccgg tgctcaccgc
      721 gcaccccacc gaggtgcaac gccagagcct gatccacaac caccgcctga tcgcgcgcct
      781 gctggacgag cgcgagcgcc agcgcctcac gcccgaggaa gccgccgaca acgacacctg
      841 cctgaccgac gccgtgctgc gcatgtggca gacccgcatg ctgcgcccgg tgcggctcaa
      901 cgtgatcgac gaggtgcgca acggcatcag ctacttcgag gaaaccttct tcaccgagct
      961 gccgcgcctg tacctgcagg ccgagcggca gttgcggcag cactttcctg gccagccctg
     1021 gcagctgccg cccctgatgc gcgtcggcag ctggatcggc ggcgaccgcg acggcaaccc
     1081 cttcgtcacc gccgagacgc tgcggcaggc catgcggctg cagtcgtcca cggcgctggc
     1141 gcgctaccag gccgaggccc acgcgctggg cagcgagctg gcgctgtccg agctgctggt
     1201 gcgccccacg ccggcgctgc tggcattggc cgaacgcgcg gccgacccct cgccgcagcg
     1261 cgccgacgag ccctaccgcc gcgcgctgat cggcatctac gcccgcctgg ccgccacgca
     1321 ccggcagctc gaccccagcg cgccgccgca gcacggcgtg gccgacgccg cgccgtatgc
     1381 cggccccgac gcgctgggcg ccgacctggc ggtgctgtcg gaatcgctgc gcagccacgg
     1441 cagcgccgcg ctggccaacg ggcggctggc gcggctgcag cgcgcggtgc aggtgttcgg
     1501 ctttcacctg gcgcccatcg acctgcgcca gaactcggac gtgcacgtgc gcaccgtggc
     1561 cgagctgctg gcgcaggccg gccgctgcgc cgactacgcg gccctgcccg aggacgcgcg
     1621 catcgcgctg ctcggcgaag aactggcctc gacccgcccg ctgcgctcgt cctacctcga
     1681 ctacagcgag gaaacgcgcg ccgagctggc catcttcgat gccgcgcgcg agattcgtca
     1741 gcagcatggc agcgccgccc tcgtcaactg catcatctcc aaggccgatg gcgtgtcgga
     1801 catgctggag ctggcgctgc tgctcaagga aagcggcctg ctgcgggccg gacccgagcc
     1861 gcgcaccgac gtcaacctga tcccgctgtt cgagaccatc gacgacctgc agcgcagcgc
     1921 ggccatcatg gacgcgctgt ttcgcctgcc cggctaccgc agcctggtgg ccacgcgcgg
     1981 cgacgagcag gaagtcatgc tcggctattc cgacagcaac aaggacggcg gctttctcac
     2041 ctcgggctgg gagctgtaca aggccgaaat cgccctggtg caggtgtgcc aggcgcatgg
     2101 cgtgcggctg cggctgtttc atggccgcgg cggctcggtg gggcgcggcg gtggcccgtc
     2161 ctaccacgcc atcctggcgc agccgccggg cgcggtgcaa ggccagatcc ggctgaccga
     2221 gcagggggag gtgatttccg ccaagtacgg cacgcctgaa accggccggc gcaacctgga
     2281 ggtgctgctg gccgccacgc tggaagccag cctggcccag caggccggcg aggcggccgc
     2341 ggcgcagcgc tttcagccgg tgatggacgc gttgtcgcag cgcgccttcg cggcctaccg
     2401 cgcgctggtg tacgagacgc cgggctttgc cacttacttc cagcagtcca cggtggtgtc
     2461 ggaaatcgcg gcgctcaaca tcggcagccg cccggcgtcg cgcaagccgt cgcagcgcat
     2521 cgaggatttg cgcgccatcc cctgggtctt cagctggtcg cagtgccggc tgatgctgcc
     2581 cggctggtac ggcttcggct cggcggtgga aggttatctg caggcgcacc cggagggcct
     2641 ggccagcctg cgcgagatgg ggcgcacgtg gtcgttcttt gccagcctgc tgtcgaacat
     2701 ggacatggtg ctggccaaga ccgacctggc catcgcctcg cgctatgccg acctggtgac
     2761 cgatgtggca ctgcgcagcc gcatcttcga gcgcatcagc gccgaatggg cgctgacgcg
     2821 gcggcatctg ctggccatcc tggagcagga cgagctgctg gccgacaacc gcacgctgcg
     2881 gcagtcgctg cagctgcgct cgccctacat ggacccgctc aaccacctgc aggtcgagct
     2941 gctcaagcgc taccgcgccg gcgacaccag cgcgcgcctg gccgccggca tccacatcac
     3001 catcaacggc atcgcctcgg gcctgcgcaa cagcggctga cgcgctggat tcaaggccaa
     3061 aacaggctcc ggcgcttgtc agtcaagcgc cagcagctat caattcaaaa gcagatcaag
     3121 ccccgaagcc gcgcgccagg aacaccgccg ccagcggaat cagcgccacc aggtgcgttg
     3181 cgatcatcac cagcttgcgc acgccgctga tctcggcctc gggcggcagg ctgccgccgg
     3241 cggccaggcg ggcctgccag cgcgcgatgc ggcgcgtggg gccgatcatg agcagcgcgg
     3301 tgataaagaa cagcgtcagc ttcaggtgca gcagccagtt gccccagtac cacgccgcgc
     3361 ccttggcgcc caggtatatg cgcgccagcc cggtcagtag cacggcggcg gtggcgatcc
     3421 acaggatgcg gtcgagccgc gccagccgct gcaccacggc ggcgttcagc cattcgcgcc
     3481 ggcacagcgc ggcctcgctc gacgcaaaca cgatccagcc caggaccgcg ctgatgtgaa
     3541 agaaagccag ccagggttca agcatggagc gatggtagcg cgtcgtgcgc tgaagtatca
     3601 aaacaatagc tgctcgcgct tgttggacgt gcgctggagc cctttttcat catgaatccg
     3661 cctgcgccgc cgcgcgccag aaggccgggt cgccatagcg ctggcgcaag aaatcaaccc
     3721 acagccgcac ccgcagcggc aggtggcgcg tggcgtgcgg caggatggcg tagatgccgt
     3781 tgggcggcgc ggcaaactct tccagcaccg gcaccagcag gccggcggcg atttcgccct
     3841 ccacctccca ggtgctgcgc cacgcgatgc cgtgcccggc gaggcaccag tcgtgcagca
     3901 cctggccatc ggaacaatcg agcgggcccc cggggcgcag gtaggtcaac tcgctctcgg
     3961 cctcgttggg cgcccccgca cccgccaggg tgcgttcgtc atctcggcca acagcatgtc
     4021 gcggcacgcc cgcacccttt ggggtgcgtt cgtcatttcg gcccacggca tttcgcg
//
LOCUS       DAOMVO010000220         4069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6346_length_4069_cov_14.019681, whole genome shotgun sequence.
ACCESSION   DAOMVO010000220 DAOMVO010000000
VERSION     DAOMVO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6346_length_4069_cov_14.019681"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /gene="rlmN"
                     /locus_tag="PKV30_10215"
     CDS             <1..813
                     /gene="rlmN"
                     /locus_tag="PKV30_10215"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRL30617.1"
                     /translation="GCRFCSTGHQGFSRNLTTGEIVAQLWFAEHFLRQHLKTDERVIS
                     NVVMMGMGEPLQNYDRLVPALRIMLDDHGYGLSRRRVTVSTSGVVPMIERLAQDCPVA
                     LAVSLHAPTDELREHLVPLNRKYPIDELLRTCTAYLAHAPRDFITFEYCMLDGVNDHP
                     EHAQALIALMRQHGADGLRAKFNLIPFNPFPQSGLVRSPMPRVQAFGKLLNEAGIVTT
                     VRKTRGDDIDAACGQLAGDVQDRTHVQERMARRRTVVLHPAPADAAQRKVVS"
     gene            810..1637
                     /gene="pilW"
                     /locus_tag="PKV30_10220"
     CDS             810..1637
                     /gene="pilW"
                     /locus_tag="PKV30_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HRL30618.1"
                     /translation="MKSLSTPLSGRTRRMCLVLGGLGLAGLLAACASQPGVGDTPAEF
                     QTASDESPARARARTRLALASGYFENGQHVVALDEQKKAVQADPTFADAYNLGGLIYM
                     ALGDNALAQSHFQRAIALNPRDANAMHNLGWLQCQQGNYAAATQSFQRAVAVPTYQDR
                     ARTLMAQGVCEARGGDPAKAEATLMHSYELDAGNPVTAYTLSQLLFQRGDFTRAQFYI
                     RRLNNSELANAQSLWLGIKVERRMNNRQAMEQLASQLRRRFPQSRELLAYERGAFDE"
     gene            1630..2505
                     /locus_tag="PKV30_10225"
     CDS             1630..2505
                     /locus_tag="PKV30_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL30619.1"
                     /translation="MSERETVPASLHEVPDSGAPRSAGQMLRELRESAGVDPALLASA
                     MKVSTQKLDALENDRLDLLPDVTFARGLASAICRAFGADPAPVLERMPVSAPGLRPPE
                     QNINQPFRRIGDRPTPVLATSFSKPLMMVVGALLLGAAALYFLPTLPIQLGAPAPAQT
                     SAEGVVREDVGPTPAVEPTPPVEMPLGAASAEQVLPPSAAAAPAAPAPAAAAGGELVQ
                     FSAKAETWVTVRDAAGKQLINRVLAAGETVGLSGDAPLSVTIGRKDAVDVTVRGQPFD
                     HRSLSKTTVSRFQVK"
     gene            2651..3919
                     /gene="ispG"
                     /locus_tag="PKV30_10230"
                     /gene_synonym="gcpE"
     CDS             2651..3919
                     /gene="ispG"
                     /locus_tag="PKV30_10230"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRL30620.1"
                     /translation="MKPGTEVPVAVAQPAARRSRQGRVVWGQRVVTVGGDAPVRVQSM
                     TNTDTVDAIGTAIQVKELALAGSELVRITVNTPEAAAQVPYIREQLDRMGVDVPLIGD
                     FHYNGHRLLTDFPDCAQALSKYRINPGNVGKGAKKDVQFGQMIEAALKWDKPVRIGVN
                     WGSLDQDLLAGLMDENAQRAQPWEARQVMYEALIQSAIGSAERAVEMGMAPEQVILSC
                     KVSGVQDLIAVYRELARRCDYPLHLGLTEAGMGTKGTVASSTALAILLQEGIGDTIRV
                     SLTPQPGEARTQEVVIASEILQSLGLRIFVPSVTACPGCGRTTSTTFQELAKQIDDRL
                     RAQMPVWRTRYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEK
                     AMTLRGEHIAQEFHAIVERYIAERFGAVSH"
BASE COUNT          693 a         1397 c         1345 g          634 t
ORIGIN      
        1 ggctgccgct tctgctccac cggccaccag ggcttcagcc gcaacctgac cacgggcgaa
       61 atcgtcgccc agttgtggtt tgccgagcat tttttgcgcc agcacctgaa gaccgacgag
      121 cgcgtcatct ccaacgtcgt gatgatgggc atgggcgagc cactccaaaa ttacgaccgc
      181 ctggtgcccg cgctgcgcat catgctggat gaccacggct acggcctgtc gcgccgccgc
      241 gtgacggtct ccacatccgg cgtggtgccg atgatcgagc gcctggctca ggattgcccg
      301 gtggcgctgg ccgtgtcgct ccacgcgccc accgacgagt tgcgcgagca cctggtgccg
      361 ctcaaccgca aatatccgat cgatgaactg ctgcgcacct gcaccgccta cctggcgcat
      421 gccccgcgcg acttcatcac ctttgaatac tgcatgctcg acggcgtgaa cgaccatccc
      481 gagcacgccc aagccttgat cgcactgatg cgccagcacg gcgcagacgg cctgcgcgcc
      541 aaattcaacc tgatcccctt caaccccttc ccgcaatccg gcctcgtgcg ctcgcccatg
      601 ccgcgcgtgc aggccttcgg caagctgttg aacgaggctg gtatcgtcac cacggtgcgc
      661 aagacacgcg gcgacgacat tgatgccgcc tgcggccagc tggccggcga cgtgcaggac
      721 cgcacccatg tgcaagagcg catggcgcgc cggcgcacgg tggtgctgca cccggctccc
      781 gcagacgctg cgcagagaaa ggttgtttca tgaagtctct ttcaacgccc ctgtcagggc
      841 gtacgcggcg gatgtgcctg gtgctgggcg gactgggtct ggcggggctg ctggccgcct
      901 gcgccagcca gcccggcgtg ggcgacacgc ctgccgagtt tcagaccgct tccgacgaga
      961 gcccggcccg cgcacgcgcg cgcacgcggc tggcgctggc ctccggctat ttcgagaacg
     1021 gccagcacgt ggtcgcgctc gacgaacaga agaaggctgt gcaggccgac ccgacctttg
     1081 ccgacgccta caacctgggc ggcctgatct acatggcgct gggcgacaac gcgctggcgc
     1141 agtcgcattt ccagcgcgcc atagcgctca acccgcgcga cgccaacgcc atgcacaacc
     1201 tgggctggct gcagtgccag cagggcaact atgcggcggc cacgcagtcg ttccagcgcg
     1261 cggtggcggt gcccacttat caagatcgtg cccggacgct gatggcgcag ggcgtgtgcg
     1321 aggcgcgcgg cggcgatccg gccaaggccg aggcgacgct gatgcattcc tacgagctgg
     1381 acgccggcaa cccggtgacg gcgtacaccc tgtcgcagct gctgttccag cgcggcgact
     1441 tcacgcgcgc ccagttctac atccggcgcc tgaacaacag cgaactggcc aatgcgcagt
     1501 cgctgtggct gggcatcaag gtcgagcggc gcatgaacaa ccggcaggcc atggagcagc
     1561 tggcgtcgca attgcgccgg cgcttcccgc aatcgcgcga gctgctggcc tacgaacgag
     1621 gtgccttcga tgagtgagcg cgagaccgtc cccgccagcc tgcacgaagt gcccgacagc
     1681 ggcgcgccgc gctcggcggg ccagatgctg cgcgagctgc gcgaatcggc cggcgtcgac
     1741 ccggcgctgc tggccagcgc catgaaggtg tcgacgcaaa agctggatgc gctggagaac
     1801 gaccgcctcg atctgctgcc cgacgtcacc ttcgcgcgcg gcctggcatc ggccatctgc
     1861 cgcgctttcg gggccgatcc ggcgcccgtg ctggagcgca tgccggtgtc cgcgcccggc
     1921 ctgcgcccgc ccgagcaaaa catcaaccag ccgtttcgac gcatcggcga ccggccgacg
     1981 ccggtgctgg ccaccagttt ttccaagccg ctgatgatgg tcgtcggcgc cttgctgctg
     2041 ggcgcggcgg ccttgtattt tttgccgacc ttgccgattc agctgggcgc cccggcacct
     2101 gcgcagacgt ccgccgaagg cgtggtgcgc gaagacgtgg gccccacgcc cgccgtcgaa
     2161 ccgacgccgc cagttgaaat gccgctgggt gccgcttcgg ccgagcaggt gttgccaccc
     2221 agcgcagccg ccgcacccgc cgcgcctgcg ccggcggccg ctgcgggcgg cgaactggtg
     2281 cagttcagcg ccaaggccga aacctgggtt acggtgcgcg acgccgccgg caaacagttg
     2341 atcaaccgcg tcctggccgc cggcgaaacg gtgggcctga gcggcgatgc gccgctgtcg
     2401 gtcaccattg gccgcaagga cgcggtcgat gtcaccgtgc gcggccagcc gttcgatcat
     2461 cggtctttga gcaagaccac cgtctcgcgt tttcaagtca aatgaactcc cccctgagtc
     2521 gccttcggcg ccatcccccc agggggacaa cgccagtggc cgggccaagc ccgctccacg
     2581 gcgttccagc atggcctgct ctgcggccac ttgacagggc ttgctgcgca gcccttggtg
     2641 aaaggttggc atgaagcccg ggacggaagt accggttgct gtggcccaac cggccgcgcg
     2701 ccgctcgcgc cagggtcgcg tggtgtgggg ccagcgcgtg gtcaccgtgg gcggcgacgc
     2761 gccggtgcgc gtgcagtcga tgaccaacac cgacacggtg gatgccattg gcaccgccat
     2821 ccaggtaaag gaactggcgc tcgccggcag cgagctggtg cgcatcaccg tcaacacgcc
     2881 cgaggcggcg gcgcaagtgc cctacatccg cgagcagctc gaccgcatgg gtgtcgatgt
     2941 gccgctgatc ggcgactttc actacaacgg gcaccggctg ctgaccgatt ttcccgactg
     3001 cgcgcaggcg ctgagcaaat accgcatcaa ccccggcaac gtgggcaagg gcgccaagaa
     3061 ggacgtgcag ttcggccaga tgatcgaagc cgccctgaag tgggacaagc ccgtgcgcat
     3121 cggcgtcaac tggggcagcc tggatcagga cctgctggcc ggcctgatgg acgagaacgc
     3181 gcagcgcgcc cagccgtggg aggcgcgcca ggtgatgtac gaggcgctga tccagtccgc
     3241 tatcggttcg gccgagcgcg ccgttgaaat gggcatggcg cccgaacagg tgattttgag
     3301 ctgcaaggtc agcggcgtgc aggatttgat agcagtctat cgagagctgg cgcgccgctg
     3361 cgactacccg ctgcacctgg gcctgaccga ggccggcatg ggcaccaagg gtacggtggc
     3421 ctccagcacg gcgctcgcca tcctgctgca agagggcatt ggcgacacca tccgcgtcag
     3481 cctgacgccg cagccgggcg aggcgcgcac gcaagaggtg gtgattgcca gcgaaattct
     3541 gcaatcgctc ggcctgcgca tcttcgtgcc cagcgtcacc gcctgccccg gctgcgggcg
     3601 caccaccagc accacgtttc aggagctggc caagcagatc gacgaccgcc tgcgcgcgca
     3661 gatgccggtg tggcgcacgc gctatccggg agttgaaaag ctgaaggtgg cggtgatggg
     3721 ctgcatcgtc aacggcccgg gcgaatcaaa gcacgccgac atcggcatca gcctgcccgg
     3781 caccggcgag gcgccggcgg cgccggtgtt catcgacggc gagaaggcta tgacgctgcg
     3841 cggcgagcac atcgcgcaag agtttcacgc catcgtcgag cgctacattg ccgagcgctt
     3901 tggcgctgtc agccactgat tcaaagcaaa aacagcctcc agtgcttttg tatcaagcgc
     3961 gggcagctat taaatagata gtattttccg aatgtcgaaa gccttgcaga tcaccgccat
     4021 caagggcatg aacgacatcc tgccgcccga atcggcgcgc tgggaatgg
//
LOCUS       DAOMVO010000221         4050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6394_length_4050_cov_13.650814, whole genome shotgun sequence.
ACCESSION   DAOMVO010000221 DAOMVO010000000
VERSION     DAOMVO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6394_length_4050_cov_13.650814"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKV30_10235"
     CDS             <1..198
                     /locus_tag="PKV30_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HRL30621.1"
                     /translation="AQAALVFIVLGMLLALLIEFAVAPRIVARENLRLWHGAGSVMYV
                     VQWLCAAAVLWRITAEPREPS"
     gene            195..1088
                     /locus_tag="PKV30_10240"
     CDS             195..1088
                     /locus_tag="PKV30_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HRL30622.1"
                     /translation="MNSPIVEIDFTDWQQAHPRPEWTAAVEAGQVLFFPKLGFELQPD
                     EKALLREDMLAAGKRNVSLGADGVLKGAAGSADEQQALAAMIGRFRAQAQGLIDRLLP
                     AYKGQLRMAPTSFRPKQVETRAQSVRADDQRMHVDAFPSRPNYGERILRVFTNLNPHG
                     APRVWRVGEDFETVARQFLPQAKPYRLWQARLLNALHVTKSLRSEYDHLMLQLHDRMK
                     FDEHYQKNGTQMTMPFPPGSVWVCFSDQATHAVMSGQFMMEQTLYLPPGREVNPQASP
                     LAILTRLVGRPLVGVGAGSAR"
     gene            complement(1167..1922)
                     /locus_tag="PKV30_10245"
     CDS             complement(1167..1922)
                     /locus_tag="PKV30_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL30623.1"
                     /translation="MKDQRQRHILCMKWGTKYGPEYVNRLYGMVRRHLGGDFNFVCLT
                     DDANGIRPEVKCLPIPPLNLQLKPGQRDGAWKKLTTFEADLHGLRGTALFLDLDVVVV
                     GPLDDFFTQPGDFLIIHDYPRFWRFGERIVGNSSVYRFELGAHADVLAYFRGHMEEVQ
                     KQYRNEQEFLSHFLHKQGKLRYWPAAWCPSFKYHGIPRWPTNYWRAPFVPPGARIVIF
                     HGEVNPPDALIGKRNKRFAHIEPALWVADAWKS"
     gene            complement(1958..2431)
                     /locus_tag="PKV30_10250"
     CDS             complement(1958..2431)
                     /locus_tag="PKV30_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HRL30624.1"
                     /translation="MPHIELSTAERKAHRAEAHHLDPVVMIGNDGLTPSVLKETDAAL
                     NAHGLIKIRVLGDDRAAREAIYSQLADELHAAPIQHIGKLLVLWRPKPKKERAEDEDR
                     MAGPKDVKVLKYSKRGGQRPEVRVVRVLGNQRLTPGGKVKKAASKQKSVKKSRGD"
     gene            complement(2662..3186)
                     /locus_tag="PKV30_10255"
     CDS             complement(2662..3186)
                     /locus_tag="PKV30_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRL30625.1"
                     /translation="MLSRRAGVLTALGIGLGVWVHLGYTLWGVGVLMRQSAWLFGAAK
                     LAGAAYLIYLGWKMLRSPPGDLAATAARPVSGLGALRTGFLTNALNRKTTVFFVSLFM
                     QVVDPQTPLGVQLAYGAFISLAHAVWFSLVALGLSAQAVRQRLLDWRRTIDRAFGGAL
                     MGLGVWLATARAGT"
     gene            3236..>4050
                     /locus_tag="PKV30_10260"
     CDS             3236..>4050
                     /locus_tag="PKV30_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30626.1"
                     /translation="MPSATRLPPLNALRCFKAAARHQHFGRAGDELHLTHGAISRAVS
                     LLEADLGLPLFERRQRRVHLTDAGRALAQSVTEALQDMRRTTERLRAQARQPRGWTMS
                     CEPTLLMRWVLRLWPGFQALHPALHFRLLAGGGAQSLDDAGIDLAVRRDDFDWPARFT
                     AEALFAERIGPVCRSAQADAWFRGGVLQAQAPRLHTRTRPGAWDDWARCTGQALPPTP
                     AEAPAQVFDHFYFSLQAAVAGLGVAMGPRHLVQDDLASGLLVAPLGFVADGSR"
BASE COUNT          664 a         1352 c         1343 g          691 t
ORIGIN      
        1 gcgcaagcag ctcttgtttt catagtgctg ggcatgttgc tggcgctgct gatcgagttc
       61 gcggtggcgc cgcgcatcgt ggcgcgggag aatctgcggc tgtggcacgg cgccggatcg
      121 gtcatgtacg tcgtgcaatg gctgtgcgcc gccgccgtgc tgtggcgcat caccgccgaa
      181 ccccgagagc cgtcatgaat tcacccatcg tcgagatcga tttcaccgat tggcagcagg
      241 cgcacccgcg gcctgaatgg acggccgccg tcgaggcggg ccaggtgctg tttttcccca
      301 agctgggctt cgagttgcag ccggatgaaa aagcgctgct gcgcgaagac atgctggccg
      361 ctggcaagcg caacgtgagc ctgggcgccg acggcgtgct gaaaggcgcg gccggcagcg
      421 ccgatgagca acaggcgctg gccgccatga tcggccgttt tcgcgcgcag gcacagggcc
      481 ttatcgaccg cctgttgccc gcgtacaagg gccagttgcg catggcgccg acgagctttc
      541 ggcccaagca ggtcgagacg cgcgcgcaat cggtgcgcgc cgacgaccag cgcatgcatg
      601 tcgatgcgtt tccgtcgcgc cccaactatg gcgagcgcat cctgcgcgtg ttcaccaacc
      661 tcaacccgca cggcgcgccg cgcgtgtggc gcgtgggcga ggatttcgag accgtggcgc
      721 gccagttcct gccgcaggcc aagccgtatc ggctgtggca ggccaggttg ctgaatgcgc
      781 tgcacgtcac caagtcgctg cgcagcgaat acgaccacct gatgctgcag ttgcacgacc
      841 gaatgaagtt cgacgagcac taccagaaaa acggcacgca gatgaccatg ccgtttccgc
      901 ccggcagcgt gtgggtctgc ttttcagacc aggcgacgca cgcggtgatg agcgggcagt
      961 tcatgatgga gcagacgctc tacctgccgc ccgggcgcga ggtgaacccg caggccagcc
     1021 cgttggcgat cctcacgcgc ctggtcgggc gcccactggt cggcgtgggt gcgggctcag
     1081 cgcgctgacg taagctacca gtttgatagc tgctcgcgct tgatggacaa gcgctggcgg
     1141 cctaaaaggc ttaaaatcgg gcgtgttcag ctcttccacg catccgccac ccacagcgcc
     1201 ggctcgatgt gcgcaaagcg cttgttgcgc ttgccgatga gcgcatcggg cgggttcact
     1261 tcgccgtgga agataacgat gcgcgcgccc ggcggcacaa agggcgcgcg ccagtaattg
     1321 gtcggccagc gcggaatgcc gtggtacttg aagctcgggc accacgcggc gggccaatag
     1381 cgcaacttgc cttgcttgtg caggaagtgc gagaggaatt cctgctcgtt gcggtattgc
     1441 ttttgcactt cttccatgtg gccgcgaaag tacgccagca catcggcgtg cgcgcccagc
     1501 tcgaagcggt agaccgacga attgccgacg atgcgctcgc caaaacgcca gaagcgcggg
     1561 taatcgtgga tgatgagaaa gtcgcccggc tgggtgaaga aatcatccag ggggccgacg
     1621 accaccacgt ccagatcgag gaacagcgcc gtgccgcgca ggccgtgcag gtcggcctcg
     1681 aaggtggtga gctttttcca cgcgccgtcg cgctggccag gcttgagctg caggttgagc
     1741 ggcgggatcg gcaggcactt cacctcgggc cggatgccgt tcgcatcgtc cgtcaggcag
     1801 acgaagttga agtcgccgcc gaggtgccgg cgcaccatgc cgtacaggcg gttgacgtat
     1861 tcggggccgt acttggtgcc ccacttcatg cacagaatgt gccgttggcg ctggtctttc
     1921 atgcgcgggc agctatcatt tcaatagttt cgaaaattca gtcaccacgg cttttcttca
     1981 ccgacttctg cttggatgcc gccttcttga ccttgccgcc gggcgtcagc cgctggttgc
     2041 ccagcacgcg caccacgcgc acttcgggcc gctggccgcc gcgcttgctg tatttcagca
     2101 ccttcacatc cttggggccc gccatgcggt cttcgtcctc ggcacgctct tttttcggct
     2161 tgggccgcca cagcaccagc agcttgccga tgtgctggat gggcgcagca tgaagctcgt
     2221 cggccaactg gctgtagatg gcctcgcgcg ccgcgcggtc gtcgcccagc acgcggatct
     2281 tgatcaggcc gtgggcgttc agcgccgcgt cggtttcctt cagcaccgag ggcgtcaggc
     2341 cgtcgttgcc gatcatgacc acggggtcca ggtggtgcgc ctcggcgcgg tgcgccttgc
     2401 gttcggcggt ggagagttca atgtgcggca tgggcgccat tatcggtgcc gttgcggtgg
     2461 gagcgccgaa aaaaccgccg gacgcgaagg acgcgaagag ctcccgaagg acgcgaaaaa
     2521 ttcagaacat caattcaaaa aaaggagctg gctgcgcttg tagcgtaggg acttaagccg
     2581 aatttcagcc tcttttctat tctttcgcgt ccttcgcgaa ttctttgcgt ccttcgcgtt
     2641 cggttgttcg ggtttcaagc gtcaggtacc ggcccgcgcc gtcgccagcc acacacccag
     2701 ccccatcagc gcgccgccaa aggcgcggtc gatggtccgc cgccagtcca gcaggcgctg
     2761 gcgcaccgcc tgtgccgaca gccccaacgc caccaggctg aaccagaccg cgtgcgccag
     2821 cgaaatgaaa gcgccatagg ccagttgcac gcccagcggc gtctgcggat cgaccacctg
     2881 catgaacagg ctgacgaaga acaccgtggt cttgcggttg agcgcgttgg tcagaaaacc
     2941 cgtgcgcaac gcgcccaggc cagacaccgg gcgtgccgcc gtggccgcca ggtcgccggg
     3001 cggcgagcgc agcatcttcc agcccaggta gatcaggtac gcggcgccgg ccagcttggc
     3061 ggcgccaaac agccaggccg attgccgcat cagcacgccc acgccccaca gcgtgtagcc
     3121 caggtgcacc cacacgccca ggccaatgcc cagcgccgtc agcacgccgg cgcggcgcga
     3181 cagcacgagg gcgtactgac caaatcagtg aattgaattc acaaaaaaaa attcgttgcc
     3241 ttcagccaca cgcctgcccc cgctgaacgc gctgcgctgc tttaaggcgg ccgcgcgcca
     3301 ccagcacttc ggccgcgccg gggacgagtt gcacctgact cacggcgcca tcagccgcgc
     3361 cgtgtcgttg ttggaggccg acctgggcct gccgctgttc gaacgccgcc agcgccgcgt
     3421 gcacctgacc gacgccggcc gcgcgctggc gcagtccgtc accgaggcgc tgcaagacat
     3481 gcgccgcacc accgaacgcc tgcgcgccca ggcgcgccag ccgcgcggct ggacgatgtc
     3541 gtgcgagccc acgcttctca tgcgttgggt gctgcggctc tggccgggtt ttcaggcgct
     3601 gcacccggcg ctgcactttc ggctgctggc cggcggcggc gcgcagtcgc tggacgacgc
     3661 aggcatcgac ctggccgtgc ggcgcgacga cttcgactgg cccgcacgct tcaccgccga
     3721 ggcgctgttt gccgagcgca tcggcccggt gtgccggtcg gcgcaggccg acgcctggtt
     3781 ccgcggcggg gtgcttcagg cgcaggcgcc gcgcctgcac acgcgcaccc gccccggcgc
     3841 gtgggacgac tgggcgcgct gcacgggcca ggcgctgccc ccgacgcccg ccgaggcccc
     3901 ggcgcaggtg ttcgatcatt tctacttcag cctgcaagcc gcagtcgccg gtctgggcgt
     3961 ggccatgggg ccacggcacc tggtgcaaga cgacctggcc agcggcctgc tggtggcgcc
     4021 gctcggcttt gtggccgatg gctcgcgcta
//
LOCUS       DAOMVO010000222         4033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6434_length_4033_cov_15.902464, whole genome shotgun sequence.
ACCESSION   DAOMVO010000222 DAOMVO010000000
VERSION     DAOMVO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_4033_cov_15.902464"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKV30_10265"
     CDS             <1..542
                     /locus_tag="PKV30_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30627.1"
                     /translation="GQRYNRETLEVLWKGKNIAQVLQMTVEEAHAFFQDQPAIARKLQ
                     TLLDVGLSYITLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEPTTGLHFADID
                     LLLKVLQQLRDAGNTIVVIEHNLDVIKTADWLIDMGPEGGAGGGTVVAQGTPEEVAAN
                     PASHTGRYLRPHLSVRAAA"
     gene            complement(573..941)
                     /locus_tag="PKV30_10270"
     CDS             complement(573..941)
                     /locus_tag="PKV30_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30628.1"
                     /translation="MTTTRWFKRIAGSGVTVALAAALGAGLFSVSTPSQAADGRYYRM
                     PDGRVEYVRDRDLRKWRRDQERRWRNSGRYGAPYDGRGSGELGDRPREWRYQNRDWSP
                     SNQWGRPEGGRYYRYPADGG"
     gene            1215..2231
                     /locus_tag="PKV30_10275"
     CDS             1215..2231
                     /locus_tag="PKV30_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL30629.1"
                     /translation="MPRRTTHPAWLHLTLMRNFLILARWCVIAWLGVAGAAHALQPLT
                     TPAELKTLAEAGGVRIVDVRDAEAYALQHLPGAVSAPYARWRGQPANPGLVPPLKDLT
                     ALVQQLGLTPEQPTVLVYTGTDSTDFATAARAYWTLKSLGARQLSILNGGLNAWRAAG
                     LPISNQAAHAPRSSWQPQFNPQWLATRAEVRQLLGSGKAVLVDSRPAPFFQGRIANDA
                     AKARGTLPGAVNIDSDLYFELGSAVLLDKASLQTEADAAHAAPGQEVVTFCNAGHWSA
                     TDWFVRSEILGEKNVKLYPGSVIDWSQAPEPLPMANEPGRWDKLRDMLTTWADRNLHF
                     IKTR"
     gene            2228..3364
                     /locus_tag="PKV30_10280"
     CDS             2228..3364
                     /locus_tag="PKV30_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRL30630.1"
                     /translation="MSTLATLPAGAPGAARRPWAQRWPLALAILALFGWLLWSVSARQ
                     ALLLLVGIGLGWGLAAARFGFTTGWRHLVEHRDPSGVYGQIILLAALSLLSMPLLAHF
                     PETQAALGPPSVSLLIGAFVFGLAMQISDGCGSGTLYKAGLGVPLNMAILPLFALGSF
                     LGSVHLNGWLALGALEPVGLVQQWGAGGALAATLAALALVAIGVGLWSGQRFSFRGTP
                     RRWIWGALVIALFAALNLFIAGQPWGVVYGFGLWGAKVATALGLFEPTQNAFWSDPGH
                     AARLHQTLLLDVTSITNIGILAGALWVAPKRPQDARPLTSAQWTIGLLAGFVLGYSSR
                     LAFGCNVGAMVSGISTGSLHGWIWVPMAFMGTLIGIRVRRRFGF"
     gene            3376..3546
                     /locus_tag="PKV30_10285"
     CDS             3376..3546
                     /locus_tag="PKV30_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30631.1"
                     /translation="MLSITALRRWIFWIGLIAFVAWDYTASAPVNLRLEPPIFAAGSG
                     APVSGGHCARPK"
     gene            complement(3615..>4033)
                     /locus_tag="PKV30_10290"
     CDS             complement(3615..>4033)
                     /locus_tag="PKV30_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HRL30632.1"
                     /translation="TRSLLIECGFHGDPSSRAVAQDMCLRFLQAAGTLDGSAPDAQLP
                     GWRLPDAPRQWALKVDGAAVARSERFAFVAPFTGLEVLAHAGTVIGHHDGPGEGEPVV
                     TPYDDCVLVMPSVRQARDGVTVVRFARRALVSPAEK"
BASE COUNT          591 a         1408 c         1392 g          642 t
ORIGIN      
        1 acggccagcg ctacaaccgc gagacgctgg aagtgctgtg gaagggcaag aacatcgccc
       61 aggtcctgca gatgacggtg gaagaggcgc acgccttctt ccaggaccag cccgcgattg
      121 cgcgcaagct gcagacgctg ctggacgtgg gcctcagtta catcacgttg ggccagagcg
      181 ccaccacgct ctcaggcggc gaggcgcagc gcgtgaagct ggcgctggaa ctcagcaagc
      241 gcgacaccgg ccgcacgctc tacatcctgg acgagccgac caccggcctg cactttgccg
      301 acatcgattt gctgctcaag gtgttgcagc aattgcgcga tgcgggcaac accatcgtgg
      361 taatcgagca caacctggac gttatcaaga ccgccgactg gctgatcgac atggggcccg
      421 aaggcggcgc tggcggtggc acggtggtgg cgcagggcac accggaagag gtggcggcga
      481 acccggccag ccacaccggg cgctacctgc gcccgcattt gtcggtacgc gcggccgcct
      541 gaaggcagcc ggccgcgcgg ccttcggcgg tatcaaccgc cgtcggccgg gtaacggtaa
      601 tagcgaccgc cctcggggcg cccccattgg ttgctggggc tccagtcacg attttgatag
      661 cgccactcgc gcgggcggtc gcccaattcg ccgctgccgc gaccgtcgta gggcgcaccg
      721 taccggccgc tgttgcgcca gcggcgctcc tggtcacggc gccatttgcg caggtcgcgg
      781 tcgcgcacgt attcgacgcg accgtcaggc atccggtaat agcggccatc ggccgcttgg
      841 ctcggcgtcg acacgctgaa caagccggcg cccagagcgg ctgccagcgc caccgtgacg
      901 ccggaaccgg cgatgcgttt gaaccaacgg gttgtcgtca tgaggctctc cttgtctgtt
      961 gcggccacag ggccgctgcc atccttcaac gccctgggcg cggcggcggt tgatcgacgg
     1021 caccccaggg tctgtgaagt caaggtaaat tccgtcagcg cctgcaccgt acggcagcac
     1081 gcggtcagat tagcccgcgc cgagcggcgc gcctgtcaga ggggctgccc cgcgactgtc
     1141 ggacaggcgg ccacggggga gatcgcttga agccacgaca atatcaacac acccacgatg
     1201 ggcgccggcc agccttgccg cgccgcacga cccacccagc ctggctgcac ctgacgctga
     1261 tgcgcaactt tcttattctt gcccgctggt gcgtgatcgc ctggctgggc gtggccggcg
     1321 ccgcgcacgc cctgcagccg ctcaccacac cggccgagct gaaaacgctg gccgaagccg
     1381 gcggcgtgcg catcgtcgac gtgcgcgatg ccgaggctta tgcgctgcag cacctgccag
     1441 gcgcggtgtc ggcgccgtat gcgcgctggc gtggtcagcc cgccaacccc ggcctggtgc
     1501 cgccgctgaa agacctgacg gcgctggtgc agcaactggg cctgacgccc gagcaaccca
     1561 ccgtgctggt ctacaccggc accgattcga ccgatttcgc caccgccgcg cgcgcctact
     1621 ggacgctgaa aagcctgggc gcgcgccagt tgtcgatcct gaacggcggt ttgaatgcct
     1681 ggcgcgccgc cggcctgccc atcagcaacc aggccgccca cgcgccgcgc agcagctggc
     1741 agccgcagtt caacccgcag tggctggcca cgcgcgccga ggtgcgccag ttgctgggca
     1801 gcggcaaggc cgtgctggtc gattcgcgcc ccgcgccctt ctttcaaggc cgcatcgcca
     1861 acgacgccgc caaggcgcgc ggcacgctgc cgggcgccgt caacatcgac agcgacctgt
     1921 acttcgaact cggcagcgcc gtgctgctcg acaaggccag cctgcagacc gaggccgacg
     1981 ccgcccacgc cgcgccgggc caggaagtgg tgaccttctg caacgccggc cactggagcg
     2041 ccaccgactg gttcgtgcgc tctgaaatcc tgggcgagaa aaacgtcaag ctctaccccg
     2101 gctcggtcat cgactggagc caggcgcccg agccgctgcc gatggcgaac gagcctggcc
     2161 ggtgggacaa actgcgcgac atgctcacca cctgggccga tcgcaacctt cacttcatca
     2221 agacgcgatg agcacactcg ccacccttcc cgccggcgcg ccgggcgccg cgcgccgccc
     2281 gtgggcgcag cgctggccgc tggcgctggc catcctggcg ctgttcggct ggctgctgtg
     2341 gtcggtgtcg gcccggcagg cgctgctgct gctggtcggc atcgggctgg gctgggggct
     2401 ggcggcggcg cgctttggct ttaccaccgg ctggcggcat ctggtggaac accgcgatcc
     2461 gtcgggcgtg tacggccaga tcatcctgct ggccgcgctg tcgctgctgt cgatgccgct
     2521 gctggcgcat ttccccgaga cgcaggcggc gctggggccg ccctcggtca gtctgttgat
     2581 cggcgccttc gtgttcgggc tggcgatgca gatttcggac ggctgcggct cgggcacgct
     2641 gtacaaggcg ggcctgggcg tgccgctcaa catggcgatc ctgccgctgt tcgcgctcgg
     2701 cagttttctg ggctcggtgc acctcaatgg ctggctggcg ctgggcgcgc tggagccggt
     2761 cggcctggtg cagcaatggg gcgcgggcgg cgcgctggcc gccacgctgg cggcgctggc
     2821 gctggtggcg attggcgttg ggctgtggag cggccagcgc ttcagtttca ggggcacgcc
     2881 gcgcaggtgg atttggggcg cgctggtgat cgcgctgttc gcggcgctga atctgttcat
     2941 cgccggccag ccctggggcg tggtctacgg cttcggactg tggggcgcca aggtggccac
     3001 cgcgctgggc ctgttcgagc cgacgcaaaa cgccttctgg agcgaccccg gccacgccgc
     3061 acgcctgcac cagacgctgc tgctggacgt gaccagcatc accaacatcg gcatcctggc
     3121 cggcgcgctg tgggtggcgc ccaagcgccc gcaggatgcg cggccgttga ccagcgcgca
     3181 gtggacgatc gggctgctgg ccggcttcgt gctgggctac agctcgcggc tggcgtttgg
     3241 ctgcaacgtc ggcgccatgg tcagcggcat cagcaccggc agcctgcacg gctggatctg
     3301 ggtgccgatg gccttcatgg gcaccttgat cggcatccgc gtgcggcggc gcttcggctt
     3361 ttgaagggac aggccatgtt gtccatcacc gcgcttcgcc gctggatttt ctggatcggg
     3421 ctgatcgcct ttgtggcgtg ggactacacc gcgtcggcgc cggtcaacct gcggctcgag
     3481 ccgcccatct tcgccgccgg ttcgggcgcg ccggtgagcg gcgggcactg cgcgcggccg
     3541 aagtgagttc gcgttttact atcaataaaa gagctgctcg cgcttgtctg acaggctcca
     3601 aagcctgatt tgattcattt ttccgccggc gacaccagcg cccgccgcgc aaagcgcacc
     3661 acggtcacgc cgtcgcgcgc ctgccgcacc gatggcatga ccagcacgca gtcgtcgtac
     3721 ggcgtgacga ccggctcgcc ttcgccgggc ccgtcgtggt ggccgatcac ggtgccggcg
     3781 tgtgccaaca cctccagccc cgtgaacggc gcgacgaacg caaagcgctc gctgcgcgcc
     3841 acggccgcgc cgtccacctt caacgcccac tggcgcggcg cgtcgggcag gcgccagccg
     3901 ggcagttgcg cgtcgggcgc ggagccgtcc agcgtgccgg ccgcttgcag aaaacgcagg
     3961 cacatatcct gcgccaccgc gcggcttgac ggatcgccgt gaaagccgca ttcgatcaac
     4021 agcgagcgcg tgt
//
LOCUS       DAOMVO010000223         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6504_length_4003_cov_15.758612, whole genome shotgun sequence.
ACCESSION   DAOMVO010000223 DAOMVO010000000
VERSION     DAOMVO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_4003_cov_15.758612"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKV30_10295"
     CDS             <1..399
                     /locus_tag="PKV30_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30633.1"
                     /translation="RARWAGLIVALAAAGLLLAWRDRVALAALVALLLLWQPGRERMA
                     GARVQPLMAWLAALSYALFLVHYPVSLMANTVFEAVMPPSPAAAAVGLLLTWAVSLGL
                     AWLLWRVLEQGSAPWQRWHAPWLGARRAAG"
     gene            396..947
                     /locus_tag="PKV30_10300"
     CDS             396..947
                     /locus_tag="PKV30_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL30634.1"
                     /translation="MTAPVVLTLPGWQGSGPDHWQSRWEALHGYTRVEQHDWLRPLRG
                     DWTARLDEVVQQQDRPVLLAAHSLGCLLTAWWAAHSRHTHRVAGALLVAPPEIERDDL
                     RQQIPGWAPPARARLPFASIVVASSDDAYGSLESASRLAADWGATFYNIGPRGHINAE
                     SGLGDWDEGLALLRSLAPAIDTR"
     gene            1016..>1511
                     /locus_tag="PKV30_10305"
     CDS             1016..>1511
                     /locus_tag="PKV30_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRL30635.1"
                     /translation="MTHPALLIIDVQQGLCEGAGAAFDGAGTITRINALSAAARAAGA
                     PVVFIQHEAQNELRHGSREWQLADGLIAESGDVRLRKATPDSFNQTELQALLNERRID
                     ALVICGMHTEFCVDTTCRRAMALGWPVWLAADAHTSAGNAALSPQQVIAHHNVTLANI
                     SSFGP"
     assembly_gap    1512..1611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1612..2024)
                     /locus_tag="PKV30_10310"
     CDS             complement(<1612..2024)
                     /locus_tag="PKV30_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRL30636.1"
                     /translation="MSDRAVRLHRVIRAQPEKLYRAFTEAEALAKWLPPHGFTCTVHH
                     LEAKVGGSFRMSFRNFTTGLAHSFGGEYLELEPGRRVRYTDRFDDPNLPGVMEVTVTL
                     TPVSCGTELAVVQAGIPEVIPLEMCYLGWQESLEQ"
     gene            complement(2072..2446)
                     /locus_tag="PKV30_10315"
     CDS             complement(2072..2446)
                     /locus_tag="PKV30_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRL30637.1"
                     /translation="MAAPHPATIDAYIAAAPAAGQPHLRQLHALLQGVAPKAEQVIKW
                     GHPFFIEPRFLFSVSAHKAHLSFAPSAEALQHFGAELKNHQTTKNFLRLPYGAPLPEA
                     LIRRIAQYRVKAVAARQDDAFW"
     gene            complement(2535..3725)
                     /locus_tag="PKV30_10320"
     CDS             complement(2535..3725)
                     /locus_tag="PKV30_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30638.1"
                     /translation="MIDLSAYRTLFADRELRRIMLGSVLPRLPIGMNALGLTLLVQAA
                     TGSFAQAGWVSGAYMAALALQAPLVGRFIDQRGPRGVMGPLAAGHTLALLGVVWAAHQ
                     GLRLPWLLGSAFVAGLFFPPVSTVIRAMYRKADMTASQRQSAFAVESVVVESCFILGP
                     LLVSLALLAGSAAYGVLLAAGFIAVGVPLFARTGAIERWGQVERTAERHWLGPLQVMG
                     VRRSLGLTLLVATGFGLNEIAVPAFATALGDTRLVGLFYAALSIPSAIAGLLYGTRRW
                     GPPLNRQILLAVLWLAAGSVAMALAPSAPWFALACLFTGLAFGPLITALSLQLGALAP
                     SSTVTEAFTWSTTLLMLGLGLGMWAGGALVQQWGWSASLLAAAGAMALAALWAPWVPA
                     VSGD"
BASE COUNT          633 a         1369 c         1371 g          530 t
ORIGIN      
        1 cgcgcgcgct gggcggggct gatcgtcgcg ctggcggccg ccggcttgct gttggcctgg
       61 cgcgaccgcg tggcgctggc ggccctggtg gcgctgctgc tgctgtggca gccggggcgc
      121 gagcgcatgg ccggcgcgcg cgtgcagccg ctgatggcct ggctggccgc gctgtcttac
      181 gcgctgtttc tggtgcacta cccggtcagc ctgatggcga acaccgtgtt tgaagccgtc
      241 atgccgccca gccctgcggc ggcggcggtc ggcttgctgc tcacctgggc ggtgagcctg
      301 gggctggcct ggctgctgtg gcgcgtgctg gagcagggca gcgcgccgtg gcagcgctgg
      361 catgcgccgt ggctcggcgc gcgaagggcc gcgggatgac ggcgcccgtc gtgctgacct
      421 tgcccggctg gcaaggcagc ggccccgatc actggcaaag ccgctgggaa gccctgcacg
      481 gctacacccg cgtggagcag cacgactggc tgcgcccgtt gcgcggcgac tggacggcgc
      541 gcctcgacga ggtggtgcag cagcaggatc gccccgtgct gctggccgcg cacagcctgg
      601 gctgcctgct caccgcctgg tgggccgcgc attcgcgcca cacgcaccga gtggccggcg
      661 cgctgctggt ggcgccgccc gagatcgagc gcgacgacct gcgccagcag atccccggct
      721 gggcgccgcc ggcgcgggcg cgcctgccct ttgccagcat cgtcgtcgcc agcagcgacg
      781 atgcctacgg cagcctcgaa tccgccagcc gcttggcggc cgattggggc gccacgtttt
      841 acaacatcgg cccgcgcgga cacatcaatg ccgaatcggg gctgggcgat tgggatgagg
      901 gactggcgct gctgcgcagc ctggcgccgg ccatcgatac ccgctgagaa aactatcaaa
      961 tcaatagctg ttggcgcagc atagacaggc gctggcggcc gatttaattc caaacatgac
     1021 ccatcccgcg ctgctcatca tcgacgtgca acaaggcctg tgcgaaggcg cgggcgcggc
     1081 gtttgacggc gcgggcacca tcacccgcat caacgccttg agcgcggccg cgcgtgcggc
     1141 gggtgcgccg gtggtcttca tccagcacga ggcgcagaac gagttgcgcc acggcagccg
     1201 tgaatggcaa ctggccgatg ggctgatcgc cgagtcgggc gatgtgcgcc tgcgcaaggc
     1261 gacgcccgac agcttcaacc aaaccgagct gcaggcgctg ctgaacgagc ggcgcatcga
     1321 cgcgctggtc atctgcggca tgcacaccga gttctgcgtc gacaccacct gccgccgcgc
     1381 gatggcgctg ggctggcccg tgtggctggc ggccgatgcg cacacctcgg ccggcaacgc
     1441 ggcgctgtcg ccgcagcagg tcatcgcgca ccacaacgtg acactggcca acatcagcag
     1501 ctttgggccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naactgctcc
     1621 agcgattcct gccagcccag gtagcacatc tccagcggaa tcacctccgg aatgccggcc
     1681 tgcaccaccg ccagctcggt gccgcacgag acgggcgtga gcgtcaccgt cacctccatc
     1741 acgccgggca ggttcgggtc gtcaaagcgg tcggtgtagc gcacgcggcg gccgggttcc
     1801 agctccagat attcgccgcc gaaggagtgc gcgaggccgg tggtgaagtt gcgaaacgac
     1861 atgcgaaagc tgccgccgac cttggcttcc aggtggtgca cggtgcaggt gaagccgtgc
     1921 ggcggcagcc acttggccag cgcctcggct tcggtgaagg cgcggtagag cttttcgggc
     1981 tgggcgcgga tgacgcggtg caggcggacg gcgcggtcgg acatgggcaa ctccggtggg
     2041 gcttcgggcc ggccagtcta cgccgcgacg gttaccagaa ggcgtcgtcc tgccgcgcgg
     2101 ccacggcctt cacgcggtat tgcgcgatgc ggcggatcag cgcctcgggc aagggcgcgc
     2161 cgtagggcag cctgaggaag tttttcgtgg tctggtgatt cttcaactca gcgccaaagt
     2221 gttgcagcgc ctcggccgag ggcgcaaaac tcaggtgcgc cttgtgcgcc gagaccgaga
     2281 acagaaaacg cggctcgatg aagaacgggt ggccccactt gatgacctgc tcggccttgg
     2341 gcgccacgcc ttgcagcagc gcgtgcagct ggcgcaggtg cggctggccg gcggcgggcg
     2401 cggcggcgat gtaggcgtca atggtggcgg ggtggggcgc ggccatgcgt tgctccaagt
     2461 ccggttgaat atgattttca tagcggcttg cgctgattgc acaagcgcca gaggccgatt
     2521 tcctgtaaaa accctcaatc ccccgacacc gccggcaccc acggcgccca cagcgcggcc
     2581 aacgccatgg cgcccgccgc cgccagcagc gacgccgacc agccccattg ctgcaccagc
     2641 gcgccgccgg cccacatgcc cagccccagg ccgagcatca gcagcgtggt gctccaggtg
     2701 aaggcttccg tcaccgtgct gctcggcgcc agcgcgccca gttgcagcga cagggcggtg
     2761 atcagcggcc cgaaggccag ccccgtgaac aggcaggcca gcgcgaacca cggcgccgac
     2821 ggcgccagcg ccatcgccac gctgccggcc gccagccaca gcacggccag cagaatctgc
     2881 cggttgagcg gcggccccca gcgccgcgtg ccgtacagca gcccggcgat ggcggatgga
     2941 atgctgagcg cggcgtagaa cagccccacc agccgcgtgt cgcccagcgc cgtggcaaag
     3001 gccggcacgg caatttcgtt cagcccgaag cccgtggcca ccagcagcgt cagccccagc
     3061 gagcggcgca cgcccatcac ctgcaagggg ccgagccagt ggcgctcggc cgtgcgctcc
     3121 acctggcccc agcgctcgat ggcaccggtg cgcgcaaaca gcggcacgcc gacggcaatg
     3181 aagcccgccg ccagcagcac gccatacgcc gccgagccgg ccagcagcgc caggctgacc
     3241 aacagcgggc cgaggatgaa gcagctttcc accaccaccg attccaccgc aaacgccgac
     3301 tgtcgctgtg acgccgtcat gtcggccttg cggtacatgg cgcggatgac ggtggagacg
     3361 ggcggaaaga acagccccgc cacaaacgcg ctgcccagca gccaaggcag ccgcaggccc
     3421 tgatgcgccg cccacaccac ccccagcagc gccagcgtgt ggccggcggc cagtggtccc
     3481 atcacgccgc gcggcccgcg ctggtcgatg aagcgcccga ccaacggcgc ctgcagcgcc
     3541 agcgccgcca tgtacgcgcc cgacacccag cccgcctgcg caaacgagcc ggtggccgcc
     3601 tgcaccagca gcgtgaggcc cagcgcgttc atgccgatcg gcaggcgcgg cagcaccgag
     3661 ccgagcatga tgcggcgcag ttcgcggtcg gcaaacaggg tgcggtaggc ggacagatcg
     3721 atcaaggcgg cgattgtagg gagccggctt ggcgcgccct ttgctacact gcgccgcagg
     3781 tagtcgcatg tgactacctt tcatcaaagc ccggagccgc cgccatgcaa gccgccgaaa
     3841 aacccgtccc caccagcctg aagctgcccg cggccgtcaa gcgcgagatc gacgaggccg
     3901 cgcgcgcggc cggcaccagc tcgcatgcgt acatgatcga cgtgttgcaa aaggcggtgg
     3961 agcggcagcg gttgcgggat cagtttcagc gggatgcgat gga
//
LOCUS       DAOMVO010000224         3994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6524_length_3994_cov_17.716425, whole genome shotgun sequence.
ACCESSION   DAOMVO010000224 DAOMVO010000000
VERSION     DAOMVO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_3994_cov_17.716425"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKV30_10325"
     CDS             <1..641
                     /locus_tag="PKV30_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HRL30639.1"
                     /translation="ALILLDIAAEPAKDGEAELEPVRIVEGHSTPARYGDQPQGADAP
                     PTLAQDQAGARTARRETPVPVVLTRFAAQNLYAPLRTVEPLPGVMRVNLRRDLDLGTL
                     MPSLPVRAAALASWRLEDQWVTAARLTNGSSSWITLDPRVLQGDFLTATFQHETLGPR
                     GTPEDTTVLYLVTRGRGLAQSLLPAIHRFDPAANLPHPEAEATDNKEARHAQ"
     gene            631..2052
                     /locus_tag="PKV30_10330"
     CDS             631..2052
                     /locus_tag="PKV30_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HRL30640.1"
                     /translation="MRSNGLLKWLMIPVALLVLFIGIRLFSGGEASAPSPTETGSRLT
                     PEEMKALGIEGDTPRDTVATLVAQVKQLRTELQTALSDNKSQREENQRLRQRENSIDQ
                     RINSALESERSNLRRDQQQAASERQQTEGLLADLQRRLDSIGGRGGGHADLPVGLGLR
                     DGDGDGEVAGMEGGVRWVEPDDAKKAEGRASSSGAMSFPTSFGPAQRTLETTAETVAN
                     AGARAAGVKTAKAVYTVPTNSTLMGSVAMTALIGRVPIDGTVNDPYPFKVLVGPDNLT
                     ANGIDIPDVAGAVFSGTASGDWTLSCVRGQVRSITFVFHDGTIRTIPEDREGNQQNNQ
                     QRDGLGWISDPYGIPCVSGERRSNAQQYLGSQALITAAGAGVASLIESDSGQMSYVGS
                     DGSIGTVGISGQEAVGRILAGGVQEMSNWVNKLYGQAFAAVYVQPGAKVAIHLEKPLA
                     IDFDPEGRKVDHRAGESHALELE"
     gene            2033..2473
                     /locus_tag="PKV30_10335"
     CDS             2033..2473
                     /locus_tag="PKV30_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HRL30641.1"
                     /translation="MLSNLNKGLALALAVAVLSGCATSKEELLTHSDRTMMDIWQQET
                     GSGGGGTGQVARRQFLDARQSLRRPLTEADVQAAPAEQMRYTRTARNEVRRQFQRLPN
                     PDLVMYVYPHLTGTDPVPVPGYTTVFPLYQRIQYAMPGERVEDY"
     gene            2473..>3994
                     /locus_tag="PKV30_10340"
     CDS             2473..>3994
                     /locus_tag="PKV30_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HRL30642.1"
                     /translation="MRWKFPWPKLAASGAGGEEQPDGWQRHVEALRQAGIPEPGSAVQ
                     GRRPATVANEQALYDVAPSFVELLPWVEFLPESKSMLLEDGQSVAAFYELVPLGTEGR
                     EPGWLAHARDALENALQDSFDELDEHPWVLQLYAQDEPSFDQYMENLRDYVQPRARGT
                     AFTEFYLRFFGHHLRAVAKPGGLFEDTVVTRLRWRGQTRRVRMVVYRRVTGHGQGQTS
                     RRGQTPEQMLGIVCDRLSGGLANAGIQARRMAAADVHDWLLRWFNPRPTLLGPRAEDR
                     ERFYALARYPDETEDGEIELVSGRDFSQRLFFGQPRSDVAHGTWYFDGMPHRVLITDR
                     LRMPPGTGHLTGEIRKGDAINTLFDQMPEDTLMCLTMVATPQDVLESDLNHLARKAVG
                     ETLASEQTLKDVHEARSLIGSAHKLYRGTLAFYLRGRDEAELDRRGLDLANVMLNAGL
                     QPVREDDEVAPLNSYLRWLPCCYNPSQDRKKWYTQLMFAQHAANLSPVWGRAQGTGH"
BASE COUNT          694 a         1358 c         1357 g          585 t
ORIGIN      
        1 gcgccttgat cctgctggac atcgctgccg agccggccaa ggacggcgaa gccgaactgg
       61 agccggtgcg catcgtcgag ggccacagca ctccggcgcg ctatggcgat cagccgcagg
      121 gtgccgacgc gcccccgacg ctcgcccagg atcaggcagg cgcgcggacg gcgcggcgcg
      181 aaacccctgt cccggtcgtg ctgacgcgct tcgccgcgca gaacctctat gcgccgctgc
      241 gcacggtcga gccgctgccg ggcgtgatgc gggtgaacct gcgccgcgac ctggacctgg
      301 gcacgctgat gccgtcgctg ccggtgcgtg cggccgcgct cgcctcctgg cgcctggagg
      361 atcagtgggt caccgccgcg cgcctcacca acggcagcag tagctggatc accctcgacc
      421 cgcgcgttct gcaaggcgat ttcctcacgg ccaccttcca gcacgagaca ctcggcccgc
      481 gcggaacgcc cgaggacacg accgtcctgt acctggtgac gcgcgggcgc ggcctggcgc
      541 agtcgctgct gccggcgatc caccgcttcg accccgccgc aaatctgccg catccggaag
      601 ccgaagccac cgacaacaag gaggctcgcc atgcgcagta acggactcct gaaatggctg
      661 atgattcccg tggcgctcct ggtgctgttc atcggcatcc ggctgttctc gggcggcgag
      721 gcctcggccc cctccccgac ggaaacggga agccggctca cgcccgaaga gatgaaggcg
      781 ctgggcatcg agggcgatac cccccgcgat accgtggcca cgctggtggc acaggtgaag
      841 caactgcgca ccgagcttca gaccgcgctg tccgacaaca agtcccagcg cgaggagaac
      901 cagcgcctgc gccagcgtga gaactcgatc gaccagcgca tcaattccgc gctcgaatcc
      961 gagcgctcca acctgcgccg cgaccagcag caggccgcga gcgagcgtca gcagaccgag
     1021 ggcctgctcg cggacttgca gcggcgcctg gacagcatcg gtggtcgcgg cggcggccac
     1081 gccgacctgc cagtgggcct ggggctgcgt gacggcgatg gcgacggcga agtggccggg
     1141 atggaaggcg gtgtgcggtg ggtggaaccg gacgacgcga agaaggccga gggccgcgcc
     1201 tcgtccagtg gtgccatgag cttccccacg agcttcggcc cggcgcagcg cacgctggaa
     1261 accacggcgg aaaccgtcgc gaacgcgggc gcgcgcgccg ccggggtcaa gaccgccaag
     1321 gctgtttata cggttccgac gaactccacc ctcatggggt cggtggcgat gacggccctg
     1381 atcggccgtg tgccgatcga cggcacggtg aacgacccgt accccttcaa ggtgttggtc
     1441 ggaccggaca acctcacggc caacggcatc gacattcccg atgtggcggg tgccgtgttc
     1501 agcggcaccg cctcgggcga ctggacactg tcgtgcgtgc gcggccaggt gcgcagcatc
     1561 acctttgtct ttcatgacgg aaccatccgc accattcccg aggatcgcga gggcaaccag
     1621 cagaacaacc agcaacgcga tggcctgggc tggatcagcg atccctatgg cattccctgc
     1681 gtcagtgggg agcggcgcag caacgcccag cagtacctcg gttcgcaggc cctgatcacg
     1741 gcggctggtg ccggcgtggc ttcgctcatc gaaagcgaca gcggccagat gtcctacgtc
     1801 ggctcggacg gctccatcgg caccgtgggc atcagcggtc aggaagccgt gggccggatt
     1861 ctcgccggtg gcgtccagga aatgtcgaac tgggtcaaca agctctacgg ccaggcgttc
     1921 gcggcggtgt acgttcagcc cggcgccaag gtcgctatcc acctcgaaaa accgctcgcc
     1981 atcgacttcg atcccgaagg ccgcaaggtc gatcaccgcg caggagaaag ccatgctctc
     2041 gaacttgaat aagggtctgg cgctggccct cgccgtcgcc gtactcagtg gctgcgccac
     2101 cagcaaggaa gaactgctga cccatagcga ccgcacgatg atggacatct ggcagcagga
     2161 aacaggtagc ggcggcggtg gcaccggtca ggtcgcacgc aggcagtttc tggatgcgcg
     2221 ccagagcctg cgccgtccgc tgaccgaagc ggacgtgcag gccgcgcccg ccgaacagat
     2281 gcgctacacg cgcaccgcgc gcaatgaggt ccgtcgccag ttccagcgtc tgccgaatcc
     2341 cgatctcgtg atgtatgtgt acccgcacct tacaggcacg gaccccgtcc ccgtgccggg
     2401 ctacacgacg gtcttcccgc tgtaccagcg catccagtac gccatgccgg gcgagcgggt
     2461 ggaggactac tgatgcgctg gaaattcccc tggccgaagc tggccgcatc cggcgctggc
     2521 ggtgaagagc agccggacgg ctggcagcgc catgtcgagg ccttgcgtca ggccggcatc
     2581 cccgaacccg ggtcggcggt ccagggccgc cggccggcga ccgtggcgaa cgaacaggcg
     2641 ctctatgacg tcgcgccgtc gttcgtggag ttgctgccct gggtggagtt cctgcccgag
     2701 tcgaagtcca tgctgctgga ggacgggcaa tcggtggcgg ccttctacga actggtgccg
     2761 ctgggcaccg agggccggga acccggctgg ctcgcgcatg cccgcgatgc gctcgaaaac
     2821 gcgctgcagg acagcttcga tgaactggac gagcacccct gggtgctgca gctctatgcg
     2881 caggacgaac ccagcttcga ccagtacatg gagaacctgc gcgactacgt gcagccgcgc
     2941 gcccgcggca cggccttcac cgagttctac cttcgcttct tcggccacca cctgcgcgcg
     3001 gtagccaagc cgggtggcct gttcgaggac acggtggtca cgcggctgcg ttggcgcggc
     3061 cagacgcggc gcgtgcgcat ggtggtctat cgccgcgtga ccgggcatgg acagggccag
     3121 acaagccgcc gtggccagac gccggagcag atgctgggca tcgtctgcga ccgcctgagc
     3181 ggtggcttgg cgaacgccgg cattcaggcc cggcgcatgg ccgcggccga cgtccacgac
     3241 tggctgctgc ggtggttcaa cccgcgcccc acactgctcg ggcctagggc cgaggaccgg
     3301 gagcgcttct atgcgctggc gcgctatcca gacgagaccg aagacggcga gattgaattg
     3361 gtgagcgggc gagatttcag ccagcggctg ttctttggcc agccgcgctc cgacgtggcg
     3421 catggcacct ggtatttcga cggcatgccg caccgcgtgc tgatcaccga tcgactgcgc
     3481 atgccgcccg gcacggggca cctaaccggc gagatccgca aaggggatgc catcaacacg
     3541 ctgttcgacc agatgcctga agacaccctg atgtgtctga cgatggtcgc cacaccgcag
     3601 gatgtcctcg aatcggacct caaccacctg gcgagaaagg ccgtcggcga gacgttggcg
     3661 tcggagcaga cgctcaagga cgtgcacgag gcccgctccc tcatcggcag cgcgcacaag
     3721 ctctaccggg gaacgctggc gttctatttg cgcggacgcg acgaagcaga gctggaccgg
     3781 cgcggactgg atctggcgaa tgtgatgctc aacgccggcc tgcagcccgt gcgcgaggac
     3841 gacgaggtgg caccgctcaa cagctacctg cgctggctgc cgtgttgcta caaccccagc
     3901 caggatcgca agaagtggta cacgcaactg atgttcgcgc agcacgcggc gaacctgtcg
     3961 ccggtgtggg gccgcgccca gggcacgggg cacc
//
LOCUS       DAOMVO010000225         3982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6545_length_3982_cov_14.782531, whole genome shotgun sequence.
ACCESSION   DAOMVO010000225 DAOMVO010000000
VERSION     DAOMVO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6545_length_3982_cov_14.782531"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKV30_10345"
     CDS             complement(<1..371)
                     /locus_tag="PKV30_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRL30643.1"
                     /translation="MNHRPPLPSAPGDAPVSRDAGSSGTPPTAPEDVGGASPAHATPG
                     VAGTPACGTSPPHESARAQLLGLAPYVDDLPEPRGTLHAAPILSPIAHGRLNGIDATA
                     ALAMPGVRALITAADIPGDPVL"
     gene            complement(368..532)
                     /locus_tag="PKV30_10350"
     CDS             complement(368..532)
                     /locus_tag="PKV30_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30644.1"
                     /translation="MNQHTPMPLPGQQPPVQDPDPFAPPPPVDDPGAPPLPRRDPGDP
                     EPVQDPEPKP"
     gene            complement(529..1995)
                     /gene="xdhA"
                     /locus_tag="PKV30_10355"
     CDS             complement(529..1995)
                     /gene="xdhA"
                     /locus_tag="PKV30_10355"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRL30645.1"
                     /translation="MSPPTLRFVLGHRPHALPDVAPDRTLLDVLREDLRCTAVKEGCA
                     SGDCGACTVAVAEAEEGGRLHWRAINSCIRLAHSVEGMAVFTAEDIAGEGGRLHPAQR
                     AMLECHGSQCGFCTPGFVMSLFTLHRQRDGAAVSRDEALHALSGNLCRCTGYRPILDA
                     AQTMHHWPDVPLDESGLLQQLKLLAQDGRAPVADSASNFYATPTTLSELLRLRAAYPQ
                     ALIAAGTTDVGLWVTKQHRRFGQIIDVTRVTELRRIERGAHSLSIGAAASLTEAFDAL
                     AESRPQLKPFFDRFAGLPVRESGTLGGNVANGSPIGDSMPLLIALGATLVLASTRGER
                     TLPIEDFYLAYRKTALAPDEVLARIEVPQPTPHEWLRADKISKRFEDDISAVCLAVAL
                     QVEDGVIHSARIGAGGVAAVPARAIQTEAALAGQSCAGATFDAAATVLEAEFKPLSDM
                     RASSAYRRAVLGNLLRRGWQQSQPGALALADLTMEPLP"
     gene            2209..3747
                     /locus_tag="PKV30_10360"
     CDS             2209..3747
                     /locus_tag="PKV30_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30646.1"
                     /translation="MNARLQLTGITKRYPGVVANDDVSLTVQPGEIHAVLGENGAGKS
                     TLMKIIYGAVKPDAGEVRFDGQPVHIRSPQEARALGIAMVFQHFSLFETLSVAENVWL
                     GLDKSLSLAEVTRRIEQTAAAYGLDIDPARPVHTLGVGEMQRVEIIRALLTNPKLLIL
                     DEPTSVLTPQAVDKLFVTLRQLASEGCSILYISHKLHEIRALCTASTVMRGGRVTGVC
                     DPREESNASLSRLMIGAEPPALAHHERTPGEVVLAVQNLSLKRDSPFGVDLVDVSLQV
                     RAGEVVGIAGVSGNGQRELLFALSGEDRRAPAASLSIANQPAGQRSPGQRRALGMHFV
                     PEERLGRGAVPEMSLADNLLLTRREAVGTLGWLRTGRLRDQAADIIERFRVKAAGPGA
                     PARSLSGGNLQKFIMGREIDAKPTLLIVSQPTWGVDVGAAAQIRAEILALRDAGCAVL
                     VVSEELDELFELSDRLHVMAKGRLSPSVARRDATVEQIGEWMSGLWHADVQQHLQQIE
                     ASHD"
BASE COUNT          607 a         1321 c         1432 g          622 t
ORIGIN      
        1 agcaccgggt cgcccggtat gtctgctgcg gtaatgagag cgcgcacccc aggcatggcc
       61 agcgccgccg tcgcatcgat gccattcagc ctgccatgcg cgatgggcga gaggatgggc
      121 gcggcgtgca gcgtgccgcg cggctcgggc aggtcatcga cgtagggcgc caggcccagc
      181 agctgcgcgc gggcgctttc gtgcgggggc gatgtgccgc aggcgggcgt gcccgccaca
      241 ccgggcgtcg cgtgagcagg cgatgcgccg ccaacgtcct cgggcgcggt gggcggcgtg
      301 ccgctgctgc cagcgtcgcg gctcaccggc gcatcgcccg gcgcggaggg caggggcggc
      361 cggtggttca tggcttgggc tcggggtctt gcaccggctc gggatcaccg gggtcgcggc
      421 gcggcagcgg cggcgcgccg gggtcgtcca ccggcggcgg cggtgcaaac gggtcggggt
      481 cttgcaccgg cggttgctgg ccgggcagcg gcatgggggt gtgctggttc atggcaaggg
      541 ttccatcgtc agatcggcca gcgcgagggc gcccggctgc gattgctgcc agccgcgccg
      601 cagcagattg cccagcacgg cgcggcggta ggcgctgctg gcgcgcatgt cgctcaaggg
      661 cttgaattcg gcttcgagca cggtagctgc ggcatcaaat gtggctccag cgcacgactg
      721 tcctgcgaga gcagcttctg tttgtatagc acgcgctggc accgccgcca cgccgccagc
      781 gccgatgcgg gcgctgtgga tcacgccatc ttcaacctgc aaggccaccg ccaggcagac
      841 ggcggagatg tcgtcttcaa agcgcttgct gatcttgtcg gcgcgcagcc attcgtgcgg
      901 cgtgggctgg ggcacctcga tgcgcgccag cacttcgtcc ggcgccagcg cggtttttcg
      961 ataggcgagg tagaagtcct caatcggcag cgtgcgctca ccgcgcgtgc tggcgagcac
     1021 cagcgtggcg cccagcgcga tgagcagcgg catgctgtcg ccgatcggcg agccgttggc
     1081 cacgttgccg cccagagtgc cgctttcgcg caccggcagg ccggcaaagc ggtcgaagaa
     1141 gggcttgagt tgcgggcgac tttcagccaa cgcgtcgaac gcctcggtca gcgatgcggc
     1201 ggcgccgatc gacagcgaat gcgcgccgcg ctcgatgcgc cgcagctccg tcacgcgcgt
     1261 cacatcgatg atctgcccga agcgccggtg ctgcttggtg acccacaaac ccacatcggt
     1321 ggtgccggcg gcgatcagcg cttgcgggta cgcagcgcgc aggcgcagca gttcgctcag
     1381 cgtcgttggc gtggcgtaga aatttgaagc tgaatcggcc actggcgccc taccatcctg
     1441 cgcaagcagc ttcaattgtt gtagcaagcc tgattcgtcc agcggcacat cgggccagtg
     1501 gtgcatggtt tgcgcggcat ccagaatcgg ccggtagccg gtgcagcggc acaggttgcc
     1561 cgacagcgcg tgcagcgcct catcacgcga caccgccgcg ccgtcgcgtt gccgatgcag
     1621 cgtgaacagg ctcatcacga agcccggcgt gcagaagccg cattgcgagc cgtggcattc
     1681 cagcatggcg cgttgcgcgg ggtgcagcct gccgccctcg cccgcgatgt cttcggcggt
     1741 gaacacggcc atgccctcca cgctgtgcgc caggcggatg cagctgttga tggcgcgcca
     1801 gtgcagccgg ccgccctcct cggcttcggc caccgccacc gtgcaggcgc cgcagtcgcc
     1861 gctggcgcaa ccttccttca ccgccgtgca gcgcaggtct tcgcgcagca cgtccagcaa
     1921 ggttcggtcg ggcgccacgt cgggcaaggc gtgcggccga tggccaagaa cgaagcgcag
     1981 ggtcggcggg ctcatgcgcg gcagtatcgc gcgaatcagg tggtgtggca cccacgccgt
     2041 gtccgcgccg tgcggcgatg gtggttgtcc atgacgcggc gccacgctga aaaacgcccc
     2101 gccggtgcta cattgcgaaa cagccgcgcc gcaaccggcg cgccacccta ctacacattc
     2161 catagctgcc cgcgctcgac ccgccacgac ccacggcctg tttgatcgat gaacgcccgc
     2221 cttcagctga ccggcatcac caagcgctac cccggagtgg tggccaatga cgacgtgtcg
     2281 ctgacggtgc agccgggcga gatccacgcc gtgctgggcg aaaacggcgc cggcaaatcc
     2341 acgctgatga agatcatcta cggcgcggtg aaacccgacg ccggcgaggt tcgctttgac
     2401 ggccagcccg tgcacattcg cagcccgcaa gaggcgcgcg cgctgggcat tgcgatggtg
     2461 ttccagcact tcagcctgtt cgagacgctg agcgtggccg agaacgtgtg gctggggttg
     2521 gacaagtcgc tctcgctggc cgaagtcacg cggcgcatcg agcagacggc ggccgcttac
     2581 ggcctcgaca tcgaccccgc gcggcccgtg cacacgctgg gcgtgggcga gatgcagcgc
     2641 gtcgagatca tccgcgcgct gctcaccaac cccaagctgc tgatcctgga cgagccgact
     2701 tcggtgctga cgccgcaggc ggtcgacaag ctgttcgtga cgctgcgcca gctggccagc
     2761 gagggttgca gcatcctcta catcagccac aagctgcacg agattcgcgc gctgtgcacc
     2821 gcctccacgg tgatgcgcgg cggccgcgtg acgggcgtgt gcgacccgcg cgaggagagc
     2881 aacgcgagct tgagccgcct gatgatcggc gccgagccgc ccgcgctggc gcaccacgaa
     2941 cgcacgccgg gcgaggtggt gctggcggtg caaaacctga gcctgaagcg cgactcgccg
     3001 tttggcgtcg acctggtcga tgtctcgctg caggtgcgcg cgggcgaggt ggtgggcatt
     3061 gccggtgtct ccggcaacgg ccagcgcgag ttgctgtttg cgctgtcggg cgaggaccgg
     3121 cgcgcgccag cagcttcgtt atccatagca aaccagccgg ccgggcagcg ttcgcccggg
     3181 cagcgccgcg cgctgggcat gcacttcgtg cccgaagagc gcctgggccg cggcgccgtg
     3241 cccgagatga gcctggccga caacctgctg ctgacgcggc gcgaagccgt gggcacgctg
     3301 ggctggctgc gcaccgggcg gctgcgcgat caggcggcgg acatcatcga gcgctttcgc
     3361 gtcaaggcgg cggggccggg ggcgccggca cgatcgctct cgggcggcaa cctgcagaaa
     3421 ttcatcatgg ggcgtgagat cgacgccaag ccgacgttgc tgatcgtctc gcaaccgact
     3481 tggggcgtcg acgtgggcgc ggcagcgcag atccgcgctg aaattttggc gctgcgcgac
     3541 gccggctgcg ccgtgctggt ggtgagcgaa gagctggacg agctgtttga gctgagcgac
     3601 cgcctgcacg tgatggccaa gggccgcctg tcgccttcgg tggcgcggcg cgacgccacg
     3661 gtggagcaga tcggcgagtg gatgagcggg ctgtggcatg ccgacgtgca gcagcatttg
     3721 cagcaaatcg aggcgtcgca tgattgaaac gctgcccatc tccgtcacgc ggcatcaaca
     3781 gaacacagag gcacagaggc acagaggaaa gaagaggtct ttctctgtcg aactctgtgc
     3841 ctctgtgcct ctgtgttctg ttcagagtca gccaccccga gaggctgtgc atgctgcgcc
     3901 ttgaaccgcg ccccgcgccc tcgcgcgcgt ggacctgggc ctcgccgctg ctggcgctgg
     3961 cgatcacggt ggtgatcggc gt
//
LOCUS       DAOMVO010000226         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6597_length_3964_cov_15.302891, whole genome shotgun sequence.
ACCESSION   DAOMVO010000226 DAOMVO010000000
VERSION     DAOMVO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_3964_cov_15.302891"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKV30_10365"
     CDS             <1..641
                     /locus_tag="PKV30_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL30647.1"
                     /translation="QYVEFILRDGARRSYSMANAPHTLIESGAPMVDLHVRHMPGGKF
                     TDHVFSAMKEKEILRIEGPYGSFHLRDDSPKPMVLLASGTGFAPIKAIIEHMQFMGIT
                     RDAVLYWGGRRPHDLYLHDWVLARCAEMPHLTYVPVVSDALAEDAWSGRNGFVHQAVL
                     QDLPDLSGHQVYACGAPIVVDSARKDYVEKAGLPSEEFYADAFTSEADKAAD"
     gene            739..1704
                     /locus_tag="PKV30_10370"
     CDS             739..1704
                     /locus_tag="PKV30_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL30648.1"
                     /translation="MQRRTLFIAGLALAAASSAFAQASKPIRLIVPYAPGGPLDVTAR
                     ALAERVEGKLGTIIVENKPGAGGNIGSDYVAKAAPDGQVIGLAAVATHAINPWLFNKM
                     PFDAAKDFAPITQMVRVPNVLVMNADTAARLKINTLADLIAYARKNPGKLNYGSGGNG
                     SAGHLAGEMFKQGAGIFAVHIPFNGGNPAQLALLSGQVDFNIDNLATASSNIKAGKLK
                     ALAVTTAGRSPLLPGVPAMSETIKGFDVGTWWGLVAPAGTPPEVINRLNAAFTEALRT
                     PETQQRFAQLMAEPVPTTPEQFGQFMARERTRYEQVVKRSGAKVD"
     gene            complement(1735..1971)
                     /locus_tag="PKV30_10375"
     CDS             complement(1735..1971)
                     /locus_tag="PKV30_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRL30649.1"
                     /translation="MSPAFKIYQDEQGSWHWQLQAGDGQVMATCGQGFTSRDACLEGV
                     AFVRTVAPLAETRDDEFPTANEGQPGVFIDRSLP"
     gene            complement(2105..3250)
                     /locus_tag="PKV30_10380"
     CDS             complement(2105..3250)
                     /locus_tag="PKV30_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL30650.1"
                     /translation="MTKTSQTTFHLSVLAAGALLATAAQAQDVQTVKIAHAGPTSGGI
                     AHIGKDTENGVRLAIDELNAQNLTIGGKKIKFEIAAEDDAGDPKQATAVAQKLCDQKV
                     AGVVGHLQSGTTIPAAGIYNKCGIPHITTAATNPDVTKPGYNTTYRLIANDNALGAAL
                     AIYAADALKLKNVAVIDDRTAYGQGLADVFKETAKKKGMNVVASEFTTDKATDFMAIL
                     TNIRGKKPDAIFYGGLDSQSGPMLRQLEQLGLGNVKFFSGDGSCTEKLPELAGKVASV
                     ANVTCATGGISVEKMAGGQDWKKRYDAKFPGAFQIYSPYAYDATMVLVDAMKRANSVD
                     PKVYVPALRKTNYKGVTAQIQFTDKGELTQPAVTLNAYKNNVRTPLN"
     gene            complement(3360..>3964)
                     /locus_tag="PKV30_10385"
     CDS             complement(3360..>3964)
                     /locus_tag="PKV30_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30651.1"
                     /translation="SNGAGKTTTMKAITGTLPLAGGSIEYLGKSIKGQGPWDLVKQGL
                     AMVPEGRGVFARMTITENLLMGAYIRNDKPGIAEDVEKIFTIFPRLRERKDQLAGTMS
                     GGEQQMLAMGRALMSRPKLLLLDEPSMGLSPIMVDKVFEVVRDVYAQGVTILLVEQNA
                     SRALALADRGYVMESGLISMSGAGKDLLADPKVRAAYLGE"
BASE COUNT          646 a         1278 c         1317 g          723 t
ORIGIN      
        1 ggcagtacgt cgagttcatc ctgcgcgacg gcgcgcggcg cagctacagc atggcgaacg
       61 cgccgcacac gctgattgaa agcggcgcgc cgatggtcga tttgcacgtg cgccacatgc
      121 ccggcggcaa gttcaccgac cacgtgttca gcgccatgaa ggagaaggaa atcctgcgca
      181 tcgaagggcc gtacggcagc tttcacctgc gcgacgattc gcccaagccc atggtgctgc
      241 tggcctcggg caccggcttt gcgccgatca aggcgatcat cgagcacatg cagttcatgg
      301 gcatcacgcg cgatgcggta ctgtactggg gcgggcgccg gccgcacgac ctgtacctgc
      361 acgactgggt gctggcccgg tgcgccgaga tgccgcacct gacatacgtg ccggtcgtgt
      421 ccgacgcgtt ggcggaagac gcctggagcg gccgcaacgg cttcgtgcat caggcggtgc
      481 tgcaggatct gcccgatctg tcgggccatc aggtgtacgc ctgcggcgcg cccatcgtgg
      541 tcgattcggc gcgcaaggac tacgttgaaa aagccggcct gcccagtgaa gagttctacg
      601 ccgacgcctt caccagcgag gcggacaagg cggctgactg aggtctggac tcgggtctca
      661 aaactatatt ttttatagct gctcgcgctt gcccatcaag cgccagcggc ctgttttact
      721 caaagaaagc actcccccat gcaacgacgc accctgttca tcgccggcct cgcgctggcc
      781 gccgcctcga gcgccttcgc gcaagccagc aaaccgattc gcctgatcgt gccctacgcc
      841 ccgggcggcc cgctcgacgt cacggcgcgt gcgctggccg agcgggtgga aggcaagctg
      901 ggcaccatca ttgtcgagaa caagcccggc gccggcggca acatcggcag cgactatgtg
      961 gccaaggccg cgcccgatgg ccaggtgatc ggcctggcgg cagtcgccac gcacgccatc
     1021 aacccgtggc tgttcaacaa gatgccgttt gacgccgcga aggacttcgc gcccatcacg
     1081 cagatggtgc gcgtgcccaa cgtgctggtg atgaacgccg acaccgccgc gcgcctgaag
     1141 atcaacacgc tggccgacct gatcgcctac gccaggaaga accccggcaa gctcaactac
     1201 ggctcgggcg gcaacggctc ggccgggcac ctggcgggcg agatgttcaa gcagggcgcg
     1261 ggcatttttg ccgtccacat ccccttcaac ggtggcaacc cggcgcaact ggcgctgctg
     1321 tcgggccagg ttgatttcaa catcgacaac ctggccaccg cgtcgtccaa catcaaggcc
     1381 ggcaaactga aggcgctggc cgtgaccacc gccgggcgct cgccattgct gcccggcgtg
     1441 cccgcgatga gcgaaacgat caagggcttc gacgtcggca cctggtgggg cctggtggcg
     1501 ccggccggca cgccgcccga ggtgatcaac cgcctgaacg ccgccttcac cgaagccttg
     1561 cgcacgcccg aaacgcagca gcgctttgcg cagttgatgg ccgagccggt gccgaccacg
     1621 cccgagcagt tcggccagtt catggcgcgc gagcggacgc gctacgagca ggtggtcaag
     1681 cgctcgggcg ccaaggtcga ttgagcgttc ctgagcggcg ccgggcgcgc gccgtcaggg
     1741 cagcgaacgg tcgatgaaaa cgccgggctg cccttcgttg gcggtcggaa actcatcgtc
     1801 acgcgtctcg gccagcggcg ccacggtgcg cacaaaggcg acgccttcca ggcaagcgtc
     1861 gcggctggta aagccctggc cgcaggtggc catcacctgg ccatcgcccg cctgcagttg
     1921 ccagtgccag ctgccttgct cgtcttgata gatcttgaat gcgggggaca tcgggtctct
     1981 cctcaagtca atatgcgcct catcttagcg agacgatgca ggttctcctg tcggccaagc
     2041 gcccataaaa aagccacgcc catgggcgtg gctggatggg cagcacgctt tctcgcgggc
     2101 cgcgtcagtt caacggcgtg cgcacgttgt tcttgtaggc gttcaaggtc acggcgggtt
     2161 gcgtcagctc gcccttgtcg gtgaactgga tctgcgccgt cacgcccttg tagttggtct
     2221 tgcgcagtgc cggcacgtag accttggggt cgacggaatt ggcgcgcttc atggcatcga
     2281 ccagcaccat cgtggcgtcg taagcgtaag ggctgtagat ctggaaggcg ccggggaact
     2341 tggcgtcgta gcgcttcttc cagtcctggc cgccggccat tttctcgacc gaaatgccgc
     2401 cggtggcgca ggtcacgttg gccacgctgg ccaccttgcc ggccaactca ggcagcttct
     2461 cggtgcacga gccatcgccg ctgaagaact tgacgttgcc caggccgagc tgctccagct
     2521 ggcgcagcat agggccggac tgcgaatcca ggccgccgta gaagatggcg tcgggcttct
     2581 tgccgcggat gttggtcagg atggccatga agtcggtcgc cttgtcggtg gtgaattccg
     2641 acgccacgac gttcatgccc ttcttcttgg ccgtttcctt gaacacgtcg gccaggccct
     2701 ggccataggc cgtgcggtcg tcgatgacgg ccacgttctt gagcttgagc gcatcggccg
     2761 cgtagatggc cagcgccgcg cccagcgcgt tgtcgttggc gatcaggcga taggtggtgt
     2821 tgtagcccgg cttggtcacg tccgggttgg tggcggcggt ggtgatgtgc ggaataccgc
     2881 acttgttgta gatgcccgcg gccggaatgg tggtgcccga ctgcaggtgg ccgaccacgc
     2941 cggccacctt ttggtcgcac agcttttgcg ccacggcggt ggcctgcttg gggtcgcccg
     3001 cgtcgtcttc agcggcgatc tcgaacttga tcttcttgcc gccgatggtc aggttctgtg
     3061 cgttcagttc gtcaatggcc aggcgcacgc cgttttcggt gtccttgccg atgtgggcga
     3121 tgccgcccga cgtgggcccg gcgtgggcga tcttgacggt ctgcacgtcc tgcgcctgag
     3181 cggcggtggc cagcagtgcg ccggcggcca gcaccgagag gtggaaggtg gtttgtgagg
     3241 ttttggtcat gcgaggtctc ctgactcaac aagggataga aacgacgatg ctgccatcgt
     3301 cttgacgaga aaaagaaatg ttacgccggg cgcgctggcg tcggttgaag cggcctgggt
     3361 cactcgccca ggtaggcggc gcgcaccttg gggtcggcca gcaggtcctt gccggccccg
     3421 ctcatgctga tcaggcccga ctccatcacg tagccgcggt cggccaaggc cagcgcgcgg
     3481 ctggcgttct gctcgaccag caggatggtg acgccctgcg cgtacacgtc gcgcaccact
     3541 tcgaacacct tgtcgaccat gatgggcgac aggcccatcg agggttcgtc gagcagcagc
     3601 agcttgggtc ggctcatcag cgcgcggccc atggccagca tttgctgctc gccacccgac
     3661 atggtgccgg cgagctggtc cttgcgctcg cgcaggcgcg ggaagatggt gaagatcttc
     3721 tccacatcct cggcgatgcc aggtttgtcg ttgcggatgt aggcgcccat gagcaggttt
     3781 tcggtgatgg tcatgcgcgc gaacacgcca cgaccctccg gcaccatggc caggccttgc
     3841 ttgaccaaat cccacgggcc ctgacccttg atcgacttgc ccaggtattc gatgctgccg
     3901 ccggccagcg gcagcgtgcc ggtgatggcc ttcatggtgg tggtcttgcc ggcgccgttg
     3961 gagc
//
LOCUS       DAOMVO010000227         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6652_length_3941_cov_15.376480, whole genome shotgun sequence.
ACCESSION   DAOMVO010000227 DAOMVO010000000
VERSION     DAOMVO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6652_length_3941_cov_15.376480"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PKV30_10390"
     CDS             complement(<1..958)
                     /locus_tag="PKV30_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL30652.1"
                     /translation="MPTYTPPLRDMQFVMHEVLNVVDQLKSIPHHADLDADTLNAVLE
                     EGGKFASEVIQPINLGGDSEGCVLDKTTHEVKTPTGFKDAYEKYVEGGWAALSCDPEY
                     GGQGLPFVTNQCLYEMLNGANQAWTMYPGLSHGAYEALHAHGTDEQKKKYLPKLTSGE
                     WTGTMCLTEPHCGTDLGLLRTKAEPQADGTYRITGNKIFISAGEHDMAPNILHLVLAR
                     LPDAPKGSKGISLFLVPKYHVNDDGSLGERNPIFCTGLEHKMGIHGNATAQLSLDGAI
                     GTLVGEPNKGLPAMFVMMNAARLGVGNQSLGLTEVAYQNALAY"
     gene            complement(1008..1670)
                     /locus_tag="PKV30_10395"
     CDS             complement(1008..1670)
                     /locus_tag="PKV30_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL30653.1"
                     /translation="MAAPRKAPALPKGQQTKAAIVDAALRMAAQVGLEGLSIGAVAEA
                     MGMSKSGVFAHFGSRDELQISVVREYYARFEHEVFEPALQQPRGLPRVRALFDHWMRY
                     TGAELDSGCIFISGAVEFDDRPGPVRDALAEAVGAWMEAMARAVAQASAEGHLQTEVD
                     AQQVTFEIHALILALHYEARFLHLPGAVDRARRGFDNILARYGSAAAVPVTSSPRGQR
                     SR"
     gene            complement(1804..2685)
                     /locus_tag="PKV30_10400"
     CDS             complement(1804..2685)
                     /locus_tag="PKV30_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRL30654.1"
                     /translation="MKLLRCGPPGKERPALLDDAGQLRDLSAIVPDIGPAQLSDAALA
                     RLRRLKPSALPLVRGRPRLGCPVAGIGKFIAIGLNYADHAAEAGMPIPKEPVVFTKAI
                     SCIQGPDDDVMLPKGSKKSDWEVELGVVIGATARHVAQKDALAHVAGYCVVNDVSERE
                     FQIERGGTWDKGKGCDTFGPIGPWLVTRDEVPNPQRLGLWLDLNGERMQTGHTRTMVF
                     SVAKLVSYVSRFITLEPGDVLTTGTPPGVGMGRRDAAGQPAPRFLRRGDVMTLGIDGL
                     GEQRQRVVAFRAAARAN"
     gene            complement(2764..3420)
                     /gene="slmA"
                     /locus_tag="PKV30_10405"
     CDS             complement(2764..3420)
                     /gene="slmA"
                     /locus_tag="PKV30_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HRL30655.1"
                     /translation="MSSAPDASADSVVTGQAPAVAARKRPKPGERRVQILQVLAEMLQ
                     QPGAERVTTAALAARLAVSEAALYRHFASKAQMFEALIDFIESSVFGLINQIGEREPA
                     GRARAARIVAMVLQFGEKNPGMARVMVGDALVFEHERLQQRMNQFFDKLDAALRQSLR
                     DGALADAAEPATLTPDADARVRASVLTAFVAGRLQRFARSGFRRAPSEQLGACIARML
                     "
     gene            complement(3711..>3941)
                     /locus_tag="PKV30_10410"
     CDS             complement(3711..>3941)
                     /locus_tag="PKV30_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRL30656.1"
                     /translation="VLDKAGALLPELTPRRIDELVQDGTISGGMLPKIAGALDAAKSG
                     VNAVHIIDGRVPHALLLEVLGDVPFGTMIRSH"
BASE COUNT          640 a         1314 c         1328 g          659 t
ORIGIN      
        1 cgtaggccag cgcgttctgg taggccactt cggtcaggcc cagcgactgg ttgccgacgc
       61 ccaagcgggc ggcgttcatc atcacgaaca tcgccggcaa gcccttgttg ggctcgccca
      121 ccagcgtgcc gatggcgccg tccaggctga gctgcgcggt ggcgttgccg tggatgccca
      181 tcttgtgctc gaggcccgtg cagaagatgg ggttgcgctc acccagcgag ccatcgtcgt
      241 tcacgtggta cttgggtacc agaaacaggc tgatgccctt gctgcccttg ggcgcatcgg
      301 gcaggcgcgc cagcaccagg tgcaggatgt tgggcgccat gtcgtgctcg ccggctgaga
      361 tgaagatctt gttgcccgtg atcctgtagg tgccgtcggc ctgcggctcg gccttggtgc
      421 gcagcagacc cagatcggtg ccgcaatgcg gctcggtcag gcacatggtg ccggtccatt
      481 caccgctggt cagcttgggc aggtatttct tcttctgctc gtcggtgccg tgggcgtgca
      541 gggcttcgta cgcgccgtgc gacaggcccg ggtacatcgt ccacgcctgg ttggcgccgt
      601 tcagcatctc gtacaggcac tggttggtga cgaagggcag gccctggccg ccgtattcgg
      661 ggtcgcacga gagcgcggcc cagccgcctt cgacgtattt ttcgtacgcg tccttgaagc
      721 cggtgggcgt cttgacttcg tgcgtggtct tgtcgagcac gcagccttcg ctgtcgccgc
      781 ccaggttgat cggctgaatc acctcgctgg cgaacttgcc gccttcttcc agcacggcgt
      841 tcagcgtgtc ggcgtccaga tcagcatggt gcgggatgct tttcagctga tcgacgacgt
      901 tgagcacttc gtgcatcacg aactgcatgt cgcgcagcgg cggggtgtag gtgggcatgg
      961 gcgatggctc cagttggggg cgaatggaaa aagcgagggt tgcgacttca gcgcgaacgc
     1021 tggccgcgcg gtgaagaggt gactgggacg gcagcggcgc tgccgtagcg cgccaggatg
     1081 ttgtcgaagc cgcggcgggc gcgatccacg gcgccgggca ggtgcagaaa gcgcgcctcg
     1141 taatgcaatg ccagaatcag cgcgtgaatc tcgaaggtga cttgttgtgc gtccacctcg
     1201 gtttgcagat gcccttcggc gctggcctgg gccacggccc gcgccatggc ttccatccag
     1261 gcgccgacgg cttcggccag cgcgtcgcgc accgggccgg gccggtcgtc gaactcgacc
     1321 gcgccgctga tgaagatgca gcctgaatcg agctcggcgc cggtgtagcg catccagtgg
     1381 tcgaacagcg cgcgcacccg cggcagcccg cgcggctgct gcagggccgg ctcgaacact
     1441 tcatgttcga agcgcgcgta gtactcgcgc accaccgaaa tctgcaactc gtcgcgcgag
     1501 ccgaaatggg cgaacacgcc ggatttgctc atgcccatgg cctcggcgac ggcgccgatg
     1561 gacaggcctt ccagcccgac ttgcgcggcc atgcgcaagg cggcgtcgac gatggccgcc
     1621 ttggtctgct gccccttggg caacgcgggc gccttgcgcg gcgcggccac aacgggatgg
     1681 agagcgggta cggaatgggg cacggcaatc aaaaaagaac gatcgttcta ttttgaccga
     1741 cattgcccgc tgcgccaaga ggcgcgcgcg ccgataaacc tagggcttac cctgatcttc
     1801 ccatcaattt gcgcgcgccg ccgcacggaa cgccaccacg cgctgccgct gttcacccag
     1861 gccgtcgatg cccagcgtca tcacgtcgcc ccgccgcaga aagcgcgggg cgggctgccc
     1921 ggccgcgtcg cggcgcccca tgccaacgcc tggcggcgtg ccggtggtca gcacgtcgcc
     1981 cggctcaagg gtgatgaagc ggctgacgta gctgaccagt ttggccacgc tgaagaccat
     2041 ggtgcgcgtg tggccggtct gcatgcgctc accgttcagg tcgagccaca ggcccaggcg
     2101 ctgcggattg ggcacctcgt cgcgcgtgac cagccagggg cctatggggc caaaggtgtc
     2161 gcagcccttg cccttgtccc aggtgccccc gcgctcgatc tggaattcac gctcgctgac
     2221 gtcgttgacc acgcagtagc cggccacatg cgccagcgcg tccttttgcg ccacatggcg
     2281 cgcggtggcg ccgatgacga cgcccaactc gacctcccag tcgctcttct tcgaaccctt
     2341 gggcagcatc acgtcgtcgt ccggcccctg aatgcacgag atggccttgg tgaagacgac
     2401 cggctccttc gggatgggca tgcccgcctc ggccgcgtgg tcggcgtagt tgagaccgat
     2461 ggcgatgaac ttgccgatgc ccgccaccgg gcagcccagg cgcgggcggc cgcgcaccag
     2521 cggcagcgcc gatggcttca ggcggcgcag acgggccagc gcggcatcgc tcagttgcgc
     2581 cgggccgatg tcgggcacga tggcggacaa gtcgcgcagc tggccggcat cgtccagcag
     2641 ggccggcctt tccttgccgg gtgggccgca gcgcagcagc ttcatggcaa cctccttgag
     2701 ccgaaaattc agatcaaaac agggctccgg cgcttgacag gcaagcgccg aaagctatat
     2761 atactatagc attctggcaa tgcacgcgcc cagttgttcg gacggcgcgc gccgaaaacc
     2821 cgagcgcgcg aagcgctgca agcggccggc cacgaaggcg gtcagcaccg aagcccgcac
     2881 tcgcgcgtcg gcatcgggcg tcaaggtggc gggctcggct gcgtcggcca gggcgccgtc
     2941 gcgcaggctt tggcgcaacg cggcgtcgag cttgtcgaag aactggttca tgcgctgctg
     3001 caggcgctcg tgctcgaaca ccagcgcgtc gcccaccatg acgcgagcca tgccggggtt
     3061 cttctcgccg aattgcagca ccatggcgac gatgcgtgcg gcgcgcgcgc ggccagccgg
     3121 ctcgcgctcg ccgatctggt tgatgagccc aaagacgctg ctctcgatga agtcgatcag
     3181 cgcctcgaac atctgcgcct tgctggcgaa atggcggtac agcgccgcct cgctcaccgc
     3241 cagccgcgcg gcgagcgcag ccgtggtgac gcgctcggca cccggctgct gcaacatctc
     3301 ggccagcacc tgcagaatct gcacgcggcg ctcacccggt ttggggcgct tgcgcgccgc
     3361 gacggccggc gcctgaccgg tgacaaccga atcggcagaa gcatcaggag cagatgacat
     3421 ggcgaataca aatgtttcca aatgattctc gcacaagcat ccgcgaccgc aagtccccaa
     3481 gcaaagtgag tgtcgcgccc gcgccgcacg acgatcaagt gagcgcttgc gccaagcgcc
     3541 tggcgaggtc tccatttgag tcgtcgtcga gtgaccgcgg agcaggacat tcgcaggcac
     3601 ccgctgcaag ggcttgtccc gcccccgctg ggtgcgcacc ccttcccgcc aagcacagcg
     3661 tagcgagaga aggggcaagc ggcgtcagcc gctcaggggg atgccccttc tcaatgcgag
     3721 cgaatcatgg tgccaaacgg cacatcgccc agcacctcca gcagcagcgc gtgcggaacg
     3781 cggccatcga tgatgtgcac cgcgttgacg ccactcttgg cggcgtcgag cgcgcccgcg
     3841 atcttgggca gcatgccgcc tgaaatggtg ccgtcctgca ccagttcgtc gatgcgccgc
     3901 ggcgtgagct ccggcagcag cgcaccggcc ttgtccagca c
//
LOCUS       DAOMVO010000228         3929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6681_length_3929_cov_14.197470, whole genome shotgun sequence.
ACCESSION   DAOMVO010000228 DAOMVO010000000
VERSION     DAOMVO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_3929_cov_14.197470"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKV30_10415"
     CDS             complement(<1..998)
                     /locus_tag="PKV30_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRL30657.1"
                     /translation="MTQITATNDFVIKFANVNGSGSASANELFAKAILRMGVPVSPRN
                     IFPSNIQGLPTWYEVRVSEKGYLGRRGGIDMMVAMNPQTWAADVEEIEPGGYLFYDST
                     RPRAASELRDDITVIGMPLTEICNAVYSDPRQRQLFKNIMYVGALSVLLDVDPAVIEA
                     LLGEQYKGKEKLIASNVQALNLGRDFATEHLKHPLSIKVEQAHRVGQRIFVDGNTAAG
                     LGAVYGGATVCAWYPITPSTSVAEAFMKYCAKFRVDADGRHDYAIVQAEDELASIGVV
                     VGAGWNGARAFTATSGPGISLMTEFIGLAYFAEIPITLVNVQRGGPSTGMPTRTQQA"
     gene            complement(995..2824)
                     /locus_tag="PKV30_10420"
     CDS             complement(995..2824)
                     /locus_tag="PKV30_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL30658.1"
                     /translation="MQPTNTANPAYFHKVVDCQWACPAHTPVPEYIRLIAQGRYGDAY
                     MVNWVSNVFPGILGRTCDRPCEPACRRGRVEEANAEKPEPVAICRLKRVAADLKEDVV
                     ARMPRPLPSNGRRIACVGAGPASLTVARDLAPLGYQVTVFEGEQKAGGFMHQQIPRFR
                     LPESVIDEETGYIFALGVEFKSGRRIESMKALLAEGYDAVFVGCGAPRGRDLDIPGRK
                     EAAEHIHIGIDWLASVSFGHVTKVGKRVIVLGGGNTAMDCCRSARRLGGEDVKVIVRS
                     GFDEMKASPWEKEDAQHEGIPIINMHVPKAFVHEGGKLTGMTFEIVRAEHDDKGRRKL
                     VPTGEPDAFFACDEVLIAVGQENAFPWIERDVGIDFDQWGLPLLREGTFQSTLPKVFF
                     GGDAAYGPKNIITAVAQGHEAAVSIDLLLDGQDVAARPAPRTNLMSEKMGIHEWAYDN
                     DPSNDLRYKVPWANAEKALASIKVEVELGFDARTAFKEAGRCLNCDVQTVFTPKLCIE
                     CDACVDICPMDCITFTVNGDEADLRQRLNAPAADTEQDLFVSPELKTGRVLVKDEDVC
                     LHCGLCAERCPTGAWDMQKFLLLTAKAGGAMNPVAASAERTAT"
     gene            3053..>3929
                     /locus_tag="PKV30_10425"
     CDS             3053..>3929
                     /locus_tag="PKV30_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRL30659.1"
                     /translation="MTPRKKKMLWTIAITVLVTVLAVVLAMNFATPEKKLDRKIEHHY
                     AISDPQYRREMSVMLGPAIVGGNRVEDLQNGDEIFPAMLKSIRAAKKTITFETYIYWS
                     GDIGKELAEALAERAKAGVKVAVLLDWVGSIKMDEDLIQTMKDAGAQVQRYRPLHWYH
                     LARMNNRTHRKLLVVDGHTAFTGGVGVAEQWTGHAQDPDHWRDMHFKVEGPVVAQFQA
                     AFNDNWIKTTGDVLNGTDYFPPSQKAGDMDAHLFISSPAGGSESMHLMYLMGIAAAEH
                     SIDLQASYFVPDELIH"
BASE COUNT          681 a         1320 c         1242 g          686 t
ORIGIN      
        1 gcctgctgcg tgcgcgtggg catgccggtg gaggggccgc cgcgctgcac gttgacgagc
       61 gtgatcggga tttcggcgaa atacgccagc ccgatgaatt ccgtcatcag cgaaatgccc
      121 ggccccgagg tggcggtgaa ggcgcgcgca ccgttccagc ccgcgcccac caccacgccg
      181 atggaggcca gctcgtcctc ggcctgcacg atggcgtagt cgtggcggcc gtcggcatcg
      241 acgcggaatt tggcgcagta cttcatgaac gcctcggcca ccgaggtgga gggcgtgatg
      301 ggataccacg cgcacaccgt ggcgccaccg tagacggccc ccaggccggc cgcagtgttg
      361 ccatcgacaa agatgcgctg gcccacgcgg tgcgcttgct ctaccttgat agacaacggg
      421 tgcttgagat gctccgtggc aaagtcgcgc cccaggttga gcgcctgcac gttggaggcg
      481 atcagctttt ccttgccctt gtactgctcg cccagcaagg cttcgatcac agcagggtcc
      541 acgtcgagca gcaccgacag cgcgcccacg tacatgatgt tcttgaacaa ctggcgctgg
      601 cgggggtcgc tgtagacggc gttgcagatc tcggtaagcg gcatgccaat gacggtgatg
      661 tcgtcgcgca gttccgacgc cgcgcgcggg cgtgtcgagt cgtagaacag atagccaccc
      721 ggctcgatct cttccacatc cgctgcccag gtctgcgggt tcatcgccac catcatgtcg
      781 atgccgccgc gccggcccag atagcctttt tcgctcaccc gcacttcgta ccaggtcggc
      841 aggccctgga tgttgctggg gaagatgttg cgcgggctga ccggcacacc catgcgcaaa
      901 atcgccttgg cgaacaactc gttggcgctg gccgagcccg agccgttgac gttggcgaac
      961 ttgatgacaa agtcgttggt ggcggtgatc tgcgtcatgt cgcggtcctt tcagcgctgg
     1021 cagctacggg attcatagcg ccgccggctt ttgcggtgag cagcagaaac ttctgcatgt
     1081 cccacgcgcc cgtggggcag cgctcggcgc acaggccgca gtgcaggcac acgtcctcgt
     1141 ccttcaccag cacgcgaccg gtcttgagtt cgggcgagac gaacaggtcc tgctcggtat
     1201 cggcggccgg cgcgttcagg cgctggcgca gatcggcctc gtcgccgttc actgtgaagg
     1261 tgatgcagtc catcgggcag atgtccacgc aggcatcgca ctcgatgcac agtttgggcg
     1321 tgaacacagt ctgcacgtcg cagttcaggc agcggccggc ttccttgaag gcggtgcgcg
     1381 catcaaagcc cagttccacc tcgaccttga tgctggccag cgccttctcg gcgttggccc
     1441 acggcacctt gtagcgcaag tcgttgctgg ggtcgttgtc gtaggcccat tcgtggatgc
     1501 ccatcttctc ggacatcagg ttggtgcgcg gcgccgggcg cgcggccaca tcctggccat
     1561 ccagcagcag gtcgatcgac accgccgcct catgcccctg cgccacggcg gtgatgatgt
     1621 tcttggggcc gtaggccgcg tcaccaccga agaagacctt gggcagcgtg gactgaaacg
     1681 tgccctcgcg cagcagcggc agaccccact ggtcgaagtc gatgccgaca tcgcgctcga
     1741 tccacgggaa ggcgttttcc tgccccacgg cgatcagcac ctcgtcgcag gcgaagaagg
     1801 catccggctc gccggtcggc accagcttgc ggcggccctt gtcgtcgtgc tcggcacgca
     1861 cgatctcgaa ggtcatgccc gtcagcttgc cgccctcatg cacaaaggct ttcggcacgt
     1921 gcatgttgat gatcgggatg ccttcgtgct gcgcgtcttc cttttcccag ggtgacgctt
     1981 tcatttcgtc gaagccgctg cgcacgatga ccttcacgtc ttcgccaccg agccgccgcg
     2041 ccgagcggca gcagtccatc gccgtgttgc caccgcccag cacgatgacg cgcttgccga
     2101 ccttggtcac gtgcccgaac gagaccgagg ccagccagtc gatgccgatg tggatgtgct
     2161 cggccgcctc tttgcggccg ggaatatcca gatcgcgccc gcgcggcgcg ccgcagccga
     2221 caaacacggc gtcgtagcct tcggccagca gcgccttcat cgactcgatg cgccggccgc
     2281 tcttgaactc gacgccgagc gcgaaaatgt agcccgtctc ttcgtcgatc acgctctcgg
     2341 gcaatcgaaa gcgcggaatc tgctggtgca tgaaaccgcc cgccttctgc tcgccttcga
     2401 acaccgtcac ctgatagccc agcggcgcca ggtcgcgcgc caccgtcagc gacgccggcc
     2461 ccgcgcccac gcaggcaatg cgcctgccgt tggacggcag cgggcgcggc atgcgcgcca
     2521 ccacgtcttc cttcaaatcg gccgccacgc gcttcaggcg gcaaatggcg acgggctcgg
     2581 gcttttcggc gttggcctct tccacccggc cgcgccggca ggcgggttcg cacggccggt
     2641 cgcaggtgcg gcccagaatg cccggaaaca cgttcgacac ccagttgacc atgtaggcgt
     2701 cgccatagcg gccctgggcg atcagacgga tgtattcggg aacgggggtg tgggcggggc
     2761 acgcccattg gcaatcgacg accttgtgaa agtaggccgg attggcggta ttcgtgggtt
     2821 gcaaaccttg tctccttgcc cgatcgattt gccacgtgtg cgcggtctag ggtgccgggt
     2881 caggccacag tctacggcag ccggccgcgc caggcggtat gcacgatcgg cccgcgcgtg
     2941 caatgttcac cctggcgccg cgttgcgcca ccgtcctacg cctgggccgc aggccgcgcc
     3001 ctagactgct cgcaaccgcc tcagcccagc cgcgccccat ccaccccggc ccatgacccc
     3061 gagaaaaaag aagatgttgt ggaccatcgc catcaccgtc ctcgtgaccg tgctggccgt
     3121 ggtgctggcg atgaatttcg ccacgcccga aaaaaagctc gaccgcaaga tcgagcacca
     3181 ctacgccatc agcgacccgc aataccggcg cgagatgagc gtgatgctcg gccccgccat
     3241 cgtcggcggc aaccgcgtcg aggatttgca gaacggcgat gaaatcttcc cggccatgct
     3301 taaaagcatt cgcgcggcca agaaaaccat caccttcgaa acctacatct actggtcggg
     3361 cgacatcggc aaggaactgg cggaagcgct ggccgagcgc gccaaggcgg gcgtgaaggt
     3421 ggccgtgctg ctcgactggg tgggcagcat caagatggac gaggacctga tccagaccat
     3481 gaaggacgct ggcgcgcagg tgcagcgcta ccgcccgctg cactggtacc acctggcgcg
     3541 catgaacaac cgcacccacc gcaagctgct ggtggtggat ggacacacgg ccttcaccgg
     3601 cggcgtgggc gtggccgagc agtggaccgg ccacgcacaa gaccccgacc attggcgcga
     3661 catgcatttc aaggtcgaag gcccggtggt ggcgcagttt caggccgcct tcaacgacaa
     3721 ctggatcaag accaccggcg atgtgctgaa cggcaccgac tactttccgc cctcgcaaaa
     3781 agccggcgac atggacgcgc acctgttcat ctcatcgccc gccggcggca gtgagagcat
     3841 gcacctgatg tacctgatgg gcattgccgc ggctgagcat tccatcgacc tgcaggcttc
     3901 gtacttcgtg cccgatgaat tgatccaca
//
LOCUS       DAOMVO010000229         3915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6712_length_3915_cov_17.716321, whole genome shotgun sequence.
ACCESSION   DAOMVO010000229 DAOMVO010000000
VERSION     DAOMVO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6712_length_3915_cov_17.716321"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PKV30_10430"
     CDS             <1..1119
                     /locus_tag="PKV30_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL30660.1"
                     /translation="AEHVAQIVSRLTGIPVNELTVEEREKLLHLEQRLHERLVGQDEA
                     VRAVADAVRLARAGLREGSKPVATFLFLGPTGVGKTELAKALAESIYGDEGALLRIDM
                     SEYGERHTVARLVGAPPGYVGYDEGGQLTEKVRRKPYSVLLLDEIEKAHPDVYNILLQ
                     VFDDGRLTDGKGRVVDFTNTIIIATSNLGSDIIQRRLKARGAAGEEYEKTKAEVMDVL
                     RGHFRPEFLNRIDEIIVFHALGKEEIRHIVGLQLDRVARNAASQGVTLTFDQTLIDHF
                     AEEGYKPEFGARELKRLIRSELETALAREMLGGGIGKADHASARWDDKAERVVFERQE
                     PPAKPAEPEKPDAANVAETPPSDASKPARKKKSAGGES"
     gene            1137..2567
                     /gene="cls"
                     /locus_tag="PKV30_10435"
     CDS             1137..2567
                     /gene="cls"
                     /locus_tag="PKV30_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRL30661.1"
                     /translation="MSDPNGLTWAAALVSLAVAISAAGHAVIYKRDPRSATLWVLLIA
                     LLPLGGSLLYLLFGINRYQRRARRLFPGADPAVQQDLSPTMPEAVSPPLAGLAHLVGR
                     ATGQSLTGGNRIEPLVDGEQAYPAMLAAIESARYSVALASYIFDSQGIGAQFVDALRR
                     AHERGVQVRVLIDDVYARWRPRSAYRALQRAGVPAATFNPTLIPARLHAAHLRNHRKL
                     LVIDGETGFTGGMNIFSPYWRPDAPEQACHDLHFRLRGPVVAHLMRCFTDDWCDTTGE
                     RLSKGFWGEPPATADEQGTSWARGIEAGPDEALDRMRWTFMGALSAAKHSVRIWTPYF
                     VPDQPMIAALSTAALRGVRVEVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPF
                     DHSKLMLIDGQWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLESLFDAARDASNEI
                     SAMALRARPLAIRLRDGVARLFTPIL"
     gene            2595..>3915
                     /gene="ftsH"
                     /locus_tag="PKV30_10440"
     CDS             2595..>3915
                     /gene="ftsH"
                     /locus_tag="PKV30_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRL30662.1"
                     /translation="MEKKDQWNIGYWIVAGLLLLTLQNYWQAAKTVEPVPYSEFEKAL
                     AEGRVAEVLVSDRTVTGRLKSPDSRGKTTIVATRVEPDLAERLSKYDVPYARVVESTW
                     LRDVLSWILPAVAFFGVWFFLFRRFAEKQGMGGFLSIGKSRAKVFMEKNTGVTFADVA
                     GVDEAKAELVEIVDFLKNPQDYGRLGARIPKGVLLVGPPGTGKTLLAKAVAGEAGVPF
                     FSISGSEFVEMFVGVGAARVRDLFEQARGQAPAIIFIDELDALGRARGVGGPIGGHDE
                     REQTLNQLLTEMDGFDSSVGLIILAATNRPEILDQALLRAGRFDRQVLVDRPDKKGRL
                     DILKVHVKKVTLAQDIDLEQVAALTTGFSGADLANLVNEAALAATRRKASAVELQDFT
                     AAIERIVAGLEKKNRVLNPKERETVAYHEMGHALVALALPGTDPVHKI"
BASE COUNT          656 a         1258 c         1343 g          658 t
ORIGIN      
        1 gccgagcatg tcgcgcagat cgtctcgcgc ctgaccggca ttccggtcaa cgagctgacg
       61 gtggaagaac gcgagaagct gctgcatctg gagcagcggc tgcacgagcg cctggtgggc
      121 caggacgaag cggtgcgcgc ggtggccgat gccgtgcgtc tggcgcgcgc gggcctgcgc
      181 gaaggcagca agccggtggc gacttttctg ttcctcgggc cgaccggcgt gggcaagacc
      241 gagctcgcca aggcgctggc cgagtccatc tatggcgatg aaggtgcgct gctgcgcatc
      301 gacatgtccg agtacgggga acgccatacc gtggcacgcc tggtgggggc gcctccgggt
      361 tatgtgggct acgacgaggg cggccagctc accgagaagg tgcggcgcaa accctacagc
      421 gtgttgctgc tggacgagat cgagaaggct caccccgacg tctacaacat cctgctgcag
      481 gtgttcgacg acgggcggct caccgacggc aagggccggg tggtggattt caccaatacc
      541 atcatcatcg ccacctcgaa cttgggctcg gacatcatcc agcgtcggct gaaggcccgt
      601 ggcgccgccg gcgaggaata cgagaagacc aaggccgagg tgatggacgt gctgcgcgga
      661 cacttccgcc ccgagttcct caaccgcatc gacgagatca tcgtcttcca tgcgctgggc
      721 aaggaggaga tccgccatat cgtcggcctg cagctcgatc gtgtggcccg caacgccgcc
      781 agccagggcg tgacgctgac cttcgatcag accttgatcg atcacttcgc ggaggaaggc
      841 tacaagcccg agttcggcgc gcgcgagctc aagcggctga tccgcagcga gctggaaact
      901 gctctggcgc gcgagatgct gggtggcggt atcggcaagg ccgatcacgc cagcgcccgc
      961 tgggacgaca aggccgaacg ggtggtcttc gagcgccagg agccacccgc gaagccggcc
     1021 gagcctgaga agcccgatgc cgcgaacgtg gccgagacgc cgccgagcga cgcgagcaag
     1081 cctgcgcgca agaagaagtc agcgggcggc gaatcttgag tcatggggag gctgtcgtgt
     1141 ccgaccccaa cgggctgaca tgggcagccg ccctcgtgtc gcttgcggtc gccatttcgg
     1201 cggcagggca cgcggtcatc tacaagcgtg acccgcgctc ggccacgctg tgggtgctgc
     1261 tgatcgctct gttgccgctg ggcggttcgc tgttgtactt gctgttcggt attaaccgct
     1321 accagcggcg cgcgcggcgg ctcttcccgg gggcagatcc tgctgttcag caggacctct
     1381 cgcccacgat gcccgaggct gtatcgccgc cgcttgccgg tctggcccac ctggtgggac
     1441 gcgccaccgg ccagtcgctg accggcggca accgcatcga gccgcttgtc gatggcgagc
     1501 aggcctaccc ggccatgctc gccgccatcg aatcggcgcg gtacagcgtc gccttggcct
     1561 cgtacatctt cgacagccaa ggcatcgggg cgcagttcgt cgatgcgctg cgccgggctc
     1621 atgagcgcgg cgtgcaagtg cgggtgctga tcgacgacgt ctatgcccgc tggaggcccc
     1681 gcagcgccta ccgcgccttg caacgcgccg gcgttccggc ggcgacgttc aacccgacgt
     1741 tgattcccgc gcgcctgcat gccgcgcatc tgcgcaatca ccgcaagctg ctggtgatcg
     1801 atggcgagac gggtttcacc ggcggcatga acatcttcag cccgtattgg cggcccgatg
     1861 cgccggagca ggcctgtcac gatttgcact ttcgtctgcg cgggccggtg gttgcgcatc
     1921 tgatgcggtg cttcaccgat gattggtgcg ataccacggg cgagcggctc agcaagggtt
     1981 tctggggcga accgcccgca acggctgatg agcaaggaac ctcttgggcg cgcggcatcg
     2041 aggccggtcc cgatgaggcc ctggacagga tgcgctggac cttcatgggc gctttgagcg
     2101 cggcgaagca ttcggtgcgc atctggacac cttacttcgt gcccgatcag ccgatgatcg
     2161 cggcgctgag cacggccgcg ttgcgcggcg tgcgcgtcga ggtgctgacg cccgcaaacg
     2221 gcgaccatcc cacggtgcaa tgggccgcgc gcgcccacta ctggcaggtg ctggagcacg
     2281 gtgtacgcat cttcgaacgg cctggcccgt tcgaccacag caagctgatg ctgatagacg
     2341 gccagtggtg ctgcctgggt tcggccaatt gggatgcgcg cagcctgcgc ctcaacttcg
     2401 agttcaatgt ggaggtgtac gacaccgcgc tgtctacgcg gctggaatcc ctttttgatg
     2461 ccgcccgtga tgcatcgaac gagatatcgg ccatggcgtt gcgcgcccgc ccgctggcca
     2521 tccgcttgcg cgacggggtg gcccgtctgt tcacccccat tctctagcgg cacgacttgc
     2581 gaggaacatt gcccatggaa aagaaagatc aatggaacat tggctactgg atcgtcgccg
     2641 gcctgttgct gctgacgctg cagaactact ggcaggcggc caagaccgtc gagcccgtgc
     2701 cctacagcga attcgagaag gcgctggccg aggggcgcgt cgccgaagtg ctggtgtcgg
     2761 accgcacggt caccgggcgc ctgaaatcgc cggacagccg gggcaagacc accatcgtgg
     2821 ccactcgcgt cgaacccgac ctggccgagc ggctgtccaa gtacgacgtg ccttacgcgc
     2881 gggtggtgga aagcacctgg ctacgtgatg tgctctcctg gattctgccg gcggtggcct
     2941 tcttcggcgt ctggttcttc ctgttccgcc gcttcgccga gaagcagggc atgggcggct
     3001 tcctgagcat cggcaagagc cgcgccaagg tgttcatgga gaagaacacc ggcgtcacct
     3061 ttgccgatgt cgctggtgtc gatgaggcca aggccgagct ggtcgaaatc gtcgacttcc
     3121 tgaagaaccc gcaggattac ggccgcctcg gcgcgcgcat cccgaaaggt gtgttgctgg
     3181 ttggcccgcc cggcacgggc aagaccctgc tggccaaggc cgtggcgggc gaggccgggg
     3241 taccgttctt ctctatctcc ggctcggagt tcgtcgagat gtttgtcggc gtgggcgcgg
     3301 cgcgggtgcg cgacctgttc gagcaggcgc gcgggcaggc gccggccatc atcttcatcg
     3361 atgaactcga cgcgctgggc cgtgcacgcg gcgtcggcgg gcccatcggc ggccacgacg
     3421 agcgtgagca gacgctcaac cagctgctca ccgagatgga cggcttcgac agctcggtgg
     3481 ggctgatcat cctcgccgcc accaaccgcc ccgaaatcct cgaccaggcg ctgctgcgcg
     3541 ccggccgctt cgaccgccag gtgctggtgg accggcccga caagaaggga cggctggaca
     3601 tcctgaaagt ccacgtcaag aaggtgacgc tggctcagga tatcgatctc gaacaggtgg
     3661 ctgcgctgac cacgggcttt tcgggtgcag acctcgcgaa cctggtcaac gaggccgcgc
     3721 tggccgcgac ccggcgcaaa gcgtccgccg tggagttgca ggatttcacc gccgccatcg
     3781 agcgcatcgt ggcgggcctg gagaagaaga accgagtact caatcccaag gagcgggaaa
     3841 ccgtggccta tcacgagatg ggccatgcgc tggtggcgct ggcgctgccc ggcaccgacc
     3901 ccgtgcacaa gatct
//
LOCUS       DAOMVO010000230         3908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6733_length_3908_cov_11.928108, whole genome shotgun sequence.
ACCESSION   DAOMVO010000230 DAOMVO010000000
VERSION     DAOMVO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_3908_cov_11.928108"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..788)
                     /locus_tag="PKV30_10445"
                     /pseudo
     CDS             complement(94..788)
                     /locus_tag="PKV30_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222573.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            904..1812
                     /locus_tag="PKV30_10450"
     CDS             904..1812
                     /locus_tag="PKV30_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30663.1"
                     /translation="MHFDLIDLRLFLHVQEAGTITGGAAASHMTLASASERIRGMEDS
                     LGSPLLSRARRGVQLTPAGRTLAHHAQLVLRQIDQLQGELGDYGAGIKGHVRLLCNSS
                     ALAEHLPQPLSAFLTAHPGVSVDLEERASDDIVDALRAGLADVGVVSDAADVEGLQAF
                     DFCADPLVLIVPAGHALAEPKSAALIDVVGQPFVGLRDGSAFQAHLDRQARRLGRRLH
                     ERVRVGSFETVCRMVALGVGVGIVPQAVASRAGGKLRRVRLRDAWARRRLPLCVRSLD
                     ELPAHARQLLQALLSAAAVKATETTN"
     gene            complement(1871..2542)
                     /locus_tag="PKV30_10455"
     CDS             complement(1871..2542)
                     /locus_tag="PKV30_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRL30664.1"
                     /translation="MPAHAAPSDGLRVLLAGASGLIGRELLQALLADPQVAVVHSLGR
                     RPLALEHPKLVQHVVDFAALPVLPALDEAYVALGTTIKQAGSQAAFRAVDFDAVVNTA
                     RAAHTAGARRLGIVSAMGADAASRVFYNRVKGEMEQAVRAVGVDTTVFARPSLLEGDR
                     STLQQPPRAGERWLHVVMRALRPVTPANLRAIEARDVALSLMDAARTRGPGAHVLLSG
                     ALQRR"
     gene            complement(2544..>3908)
                     /locus_tag="PKV30_10460"
     CDS             complement(2544..>3908)
                     /locus_tag="PKV30_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRL30665.1"
                     /translation="MLDMGFIHDVKKVLALLPKHKQSLLFSATFSDEIRELAGNLLKD
                     PKSVQVTPPNTTVERIAQVIYPVGRNRKKDVLAHLVGENDWSQVLVFTRTKFGANKVA
                     DFLNDKGIKSMALHGNKSQSARTQALAEFKTGDLRALVATDIAARGIDIDELPHVVNY
                     DIPNVPEDYVHRIGRTGRAGNSGEAVSLVCMDEEGFMHEIERFTKQQIPVRMLDGFGP
                     EEGERAEPIAMGRQTLWGGLGRPPSREVMAAAAKAARQEMMQRIRENKAGQGPRGGGA
                     GGRGPQPARKPQPQRPPDGMARADGAVELDADGLPMQQLGERTGRNRRRRGRGGNAGG
                     APQQNPRANGQGQGQNRPAPQATRDFGFDDEDDRDIDHLPRHIDPLQTNLHGRRSAPR
                     GPSHGAVGQPDPMRTSIDLMGKGKGGGGGGRSRSGGGGGYGGNRSGGGGGVGNRRGGG
                     GYGR"
BASE COUNT          565 a         1369 c         1367 g          607 t
ORIGIN      
        1 attccggctt ttgccggaat gacggtggca ggcaggattt agtgggtttt ctggtgtcgc
       61 gcctgaaata gagggcccat caattgattt gactcacaac gccagcagca ccatccacgc
      121 tcccagcagc gccagcgcgc gcatgaagca cagccggcaa cagcatcagc gccgagccgc
      181 tcagcgccgc cgccgaacac tggccgttca gcatcagccc agccgccagc gccacggtgg
      241 aaacggtgaa cgacagcccg atcgcctgca ccagctcgtc gcgcgacaga ccgaacgcct
      301 gcagataggg cacggccgga atcacgaaca cccccgtggg ccgtgaccag cccggtgaca
      361 gcgcccacca gcgggcccag cacgcgctcg gcgccggccg gcacttgcgg cacccgcgcg
      421 cccgacaggc cccacagccc atacaccacc agcgccgccc ccagcgcctg catggcccag
      481 gcgccggccg gcgcgcccag cagctacgcg ccgcccagcg tgccgacgac cacgcccgcc
      541 tgcatgggcc acaggcgccg cgtcatcggc gccagcgtgg gccacggccg catctgccag
      601 acgttggtca ccaccgaggg caggatcagc agcgccgccg cctgcgccgg cggcatggcc
      661 agcgccagca gcgccatcgc cagcgtcggc agacccaggc ccaccacgcc cttgaccagc
      721 ccggccagca ggaaggcggt ggtggtgagc agcatcaggg aaagactgag atggggcagg
      781 gtggtcatgg tgctggcctg gtggatggcg ccattgtttc ctctgaagcg tcggccgaca
      841 atgcggaatt ggcgtggcca gcctcaggcc atgcctgatt cacaaaaagc gaaggagatg
      901 gcgatgcact tcgacctcat cgacctgcgc ctgtttctgc acgtgcagga agccggcacc
      961 atcaccgggg gcgcggcggc cagccacatg acgctggctt cggccagcga gcgcattcgc
     1021 ggcatggagg attcgctcgg ctcgccgctg ctgtcgcgtg cgcggcgtgg cgttcaactg
     1081 acgccggccg gccgcaccct ggcgcaccac gcgcaattgg tgctgcggca aatcgaccaa
     1141 ctgcagggcg aactgggtga ctacggcgcc ggcatcaagg gccatgtgcg gctgctgtgc
     1201 aacagctcgg cgctggcgga gcacctgccg cagccgctga gtgcgttcct cacggcccat
     1261 ccgggcgtct cggtcgatct ggaagagcgg gcgagcgacg acatcgtcga tgccttgcgc
     1321 gccggcctgg ccgatgtggg cgtggtgtcg gatgcggccg atgtcgaggg gctgcaggcg
     1381 tttgatttct gcgccgatcc gctggtgctg atcgtgccgg ccggccatgc gctggccgag
     1441 ccaaagtcgg cggcactgat cgacgtggtg ggccagccct tcgtcggcct gcgggacggc
     1501 agcgcctttc aggcgcatct ggatcggcag gcccggcggc tcggccgtcg cctgcacgag
     1561 cgcgtgcgcg tcggcagctt cgagacggtg tgccgcatgg tagcgctggg cgtcggcgtg
     1621 ggcatcgtgc cgcaggccgt ggcctcgcgc gccggcggaa agctgcggcg cgtgcggctg
     1681 cgcgacgctt gggcgcggcg ccgcttgccg ctgtgcgtgc gatcgcttga cgagctgccg
     1741 gcgcacgcgc gccagctgct gcaggcgctg ctgtccgccg ccgcggtcaa ggcgactgaa
     1801 actacaaatt agatagctgc tcgcgcttaa ctgataagcg ccagcgcccg aaccgatcaa
     1861 taaacggtga tcaacggcgc tgcagtgcgc ccgacagcag cacgtgcgcg ccagggcctc
     1921 gcgtgcgtgc ggcatccatc agactgagcg ccacgtcgcg cgcttcaatg gcgcgcaggt
     1981 tggcgggcgt gaccgggcgc agcgcacgca tgaccacgtg caaccagcgc tcgcccgcgc
     2041 gcggcggttg ttgcagcgtg gagcgatcgc cttccagcag cgacgggcgg gcgaataccg
     2101 tggtgtcgac gcccacggcg cgcaccgctt gctccatctc gcccttgacg cggttgtaga
     2161 acacgcgcga tgcggcgtcg gcgcccatgg cgctgacaat gcccagccgg cgggcgccag
     2221 cggtgtgggc agcgcgcgcg gtgttcacca ccgcatcgaa atcgacggcg cgaaacgccg
     2281 cctggctgcc ggcctgcttg atggtggtgc cgagcgccac gtaggcctca tccagcgccg
     2341 gcaagactgg cagcgcagcg aagtcgacga cgtgctgaac cagcttggga tgttcgaggg
     2401 ccagcggccg gcggcccagg ctgtgcacca cggccacctg cggatcggcc agcaaggcct
     2461 gcagcagttc gcgcccgatc aggcccgaag cgccggccag cagcacgcgc aagccatcag
     2521 acggcgcagc gtgcgcaggc acgtcagcgc ccgtaacctc cgccgccgcg gcggttgcca
     2581 acaccgccgc cgccgccact gcggttgccg ccgtagccgc caccgccacc ggagcggctg
     2641 cgaccaccgc cgccaccgcc tttgcctttg cccatcagat cgatgctggt gcgcatcggg
     2701 tcgggttggc ccacggcccc gtggctgggg ccgcgcggcg cgctgcggcg gccgtgcagg
     2761 ttggtctgca gcgggtcgat gtggcgcggc agatggtcga tgtcgcggtc gtcttcgtcg
     2821 tcaaaaccga agtcgcgcgt ggcctgtggc gccgggcggt tctggccctg gccttggccg
     2881 ttggcgcgcg gattctgttg cggcgcgcct ccggcgttgc cgccgcgccc acggcgacgg
     2941 cggttgcggc cggtgcgctc gcccagctgt tgcatcggca gaccgtcggc gtccagctcg
     3001 acggcgccgt cggcacgcgc catgccgtcg ggcgggcgct gcggctgcgg cttgcgcgcc
     3061 ggctgcgggc cacgtccacc agcgccgcca ccgcgcgggc cctggccggc cttgttctcg
     3121 cggatgcgtt gcatcatctc ctggcgcgcg gccttggcgg cggcggccat cacctcgcgg
     3181 ctcggcgggc ggcccaggcc gccccacagc gtctggcggc ccatggcgat gggctcggcg
     3241 cgctcgcctt cctcggggcc aaagccgtcc agcatgcgca cgggaatctg ctgcttggtg
     3301 aagcgctcga tctcgtgcat gaagccttcc tcgtccatgc acaccaggct cactgcctcg
     3361 ccgctgttgc cggcgcggcc ggtgcggccg atgcggtgca cgtagtcttc gggcacgttg
     3421 ggaatgtcgt agttgacgac gtgcggcagc tcgtcgatgt cgatgccgcg tgccgcaata
     3481 tcggtagcaa ccagggcgcg cagatcgccc gtcttgaact cggccagcgc ctgggtgcgg
     3541 gcgctctggc tcttgttgcc gtgcagcgcc atggacttga tgcccttgtc gttcaaaaag
     3601 tcggccacct tgttggcgcc gaacttggtg cgcgtgaaca ccagtacctg gctccagtcg
     3661 ttttcgccga ccaggtgcgc cagtacgtct ttcttgcggt tgcgacccac ggggtagatc
     3721 acctgcgcga tgcgctccac cgtggtgttg ggcggcgtga cctgcacgct cttcgggtcc
     3781 ttcagcaggt tgcccgccag ttcgcggatt tcgtcgctga aggtggcgct gaagagcagg
     3841 ctttgcttgt gcttgggcag cagcgccagc accttcttca cgtcgtggat gaagcccatg
     3901 tcgagcat
//
LOCUS       DAOMVO010000231         3898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6756_length_3898_cov_12.518865, whole genome shotgun sequence.
ACCESSION   DAOMVO010000231 DAOMVO010000000
VERSION     DAOMVO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6756_length_3898_cov_12.518865"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..312
                     /locus_tag="PKV30_10465"
     CDS             61..312
                     /locus_tag="PKV30_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRL30666.1"
                     /translation="MKNLEVSTSDGAPKKTGTMTIQLDGRPHQVGAGSTLADLVAALG
                     HAPEAVSTAVNGEFVARHARGRALAECDAVLLFQPIVGG"
     gene            380..1807
                     /gene="arcD"
                     /locus_tag="PKV30_10470"
     CDS             380..1807
                     /gene="arcD"
                     /locus_tag="PKV30_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HRL30667.1"
                     /translation="MSAPVQLRLGALTALVVGSMVGGGIFSIPQNTAASAGVGATAVA
                     WGITGLGMLALAFVFLNLALRQPHLDSGIYAYARAGFGPFTGFSSAWGYWFMAVLGNV
                     GYYVLLFSTLGHYLPAFGDGNTPLAVACASVMLWGTHALVLRGIKEAARINQITTVAK
                     LVPLLLFVLLVALAFKLDVFRADVWGRANPQLGSVLDQVRGMMLVTVWVFIGVEGASV
                     YSAHAARRGDVGRATLAGFLFVLALLVAVSLLSMGVMSQPELAHLKNPSMAHVLERAV
                     GPWGSALISVGLLISLSGALLSWLMLSAETLYSAARDGSAPSWLAGQNARGVPSNALW
                     LTSGSVQLFLLITLVSNSTYLSLVNLGTAMILLPYTWAAGYAMKIALRGDGYGVADGR
                     LRQRDAAVSALALLYAVWLVYAGGVKYLLLGALLYAPGAVLYAWARREHGKRVFSRRE
                     WWGFAGLLLAAGVAALGLQQRWLSL"
     gene            1883..2683
                     /locus_tag="PKV30_10475"
     CDS             1883..2683
                     /locus_tag="PKV30_10475"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRL30668.1"
                     /translation="MSSTDSWRVGDLQLTSRFLLGTAGYPSPQVLADAIAASGTQIVT
                     VGLKRQLAAAGDNGFVDIIRSAMKTHSARLLPNTAGCTTAREAIQLAHMARELYGTPW
                     VKLEVVGDEYTLQPDPVELVEAARQLSKDGFVVFPYCTDDLVTCKRLLDAGCPLLMPW
                     GAPIGSGQGLVNPFALRLLRERLSDVPLIIDAGIGAPSHAAQALEMGFDAVLLNSAVS
                     QASDPVRMAAAFKAAIEAGRTARLAGVMAKQDFAVATTPVAGNPFLIN"
     gene            2689..>3898
                     /locus_tag="PKV30_10480"
     CDS             2689..>3898
                     /locus_tag="PKV30_10480"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRL30669.1"
                     /translation="MVPVLDPPAPAAPDVVAKPIIWSIAGNDSGGGAGLSADQRAAEA
                     FGVHLCPVVAAITAQSSVGVARVEAVSPEVLDAQLAALADDMPPAAIKTGLLGSAGTV
                     AVVARWVDKLRARGPVALVVDPVLGASTGAAFADDAVLRAYRELLLPRATLLTPNERE
                     AGRLVGEGGGEDAPQPLTPALSPNGRGSEQPGSFQSDAAVLPHLPAGERAGVRGTAHP
                     APVAVVPALAQALRAQGTQAVAITGGDSAPAPGWSLDWIDTPHASGWLALPRIDTRHT
                     HGTGCTFATSAACAMALGFVAADALVLAKMATTHALRHGYAAGQGAGPVAARGGFAND
                     PALMPIMSWGDDSDFDSCLRLIDKRQSAISSKNANLDLYGIVDSAERVQQVVQAGVKL
                     VQLRIKTPDAP"
BASE COUNT          554 a         1249 c         1408 g          687 t
ORIGIN      
        1 gttcgatgat cttgctatga attcgaaagc tgccagcgca ggactggcaa gcgccatcgc
       61 atgaaaaacc ttgaagtctc cacctcggac ggcgcaccca agaagaccgg cacgatgacc
      121 atccaactcg acggccgacc gcaccaagtg ggggccggca gcacgctggc cgacctggtg
      181 gccgcgctcg gccatgcgcc cgaagccgtc agcacggcag tcaacggcga gttcgtcgcc
      241 cgtcacgcgc gtgggcgggc gctggccgag tgcgacgcgg tcttgctgtt tcagcccatc
      301 gtcggcggtt gagcgcgggc ggcgcctgcc gcgcaggtaa agttgctcac cccccaagaa
      361 gaccctgccc agacccgaca tgtccgctcc cgttcaactg cgccttggcg cgctcaccgc
      421 cctggtggtc ggctcgatgg tcggcggcgg catcttctcg attccgcaga acaccgcggc
      481 cagcgcgggg gtgggcgcca cggccgtcgc ctggggcatc accgggttgg gcatgctggc
      541 gctggcgttt gtgttcctca atctggcgtt gcgccagccg caccttgatt caggcattta
      601 cgcctatgcg cgcgccggct tcgggccgtt cacggggttc tcgtcggcgt ggggttactg
      661 gttcatggcg gtgctgggca acgtgggtta ctacgtgctg ctgttttcca cgctgggcca
      721 ctacctgccg gcgtttggcg atggcaacac gccgctggcg gtggcgtgcg cgtcggtgat
      781 gttgtggggc acgcacgcgc tggtgctgcg cggcatcaag gaggcggcgc gcatcaacca
      841 gatcaccacc gtggccaagc tggtgccgct gctgttgttc gtgctgctgg tggcgctggc
      901 gttcaagctg gacgtgtttc gcgccgacgt gtggggccgc gccaatccgc agttgggcag
      961 cgtgctggac caggtgcgcg ggatgatgct ggtcaccgtg tgggtgttca tcggcgtcga
     1021 aggcgccagc gtgtattcgg cccacgccgc gcgacgcggt gacgtaggac gcgccacgct
     1081 ggcgggtttc ttgttcgtgc tggcgctgtt ggtggcggtg agtttgctgt cgatgggtgt
     1141 gatgtcgcag cctgaattgg cgcacctgaa gaacccgtcg atggcgcacg ttctggagcg
     1201 cgccgtcggc ccgtggggca gcgcgctgat cagcgtgggg ctgctgattt cgctgtcggg
     1261 cgcgctgctg tcgtggctga tgctgtcggc cgagacgctg tattccgccg cgcgcgacgg
     1321 ctcggcgccc agctggctgg cggggcagaa cgcgcgcggc gtgccgtcca acgcgctgtg
     1381 gctgaccagc ggcagcgtgc agctgtttct gctgatcacg ctggttagca actcgactta
     1441 tctgtcgctg gtcaatctgg gcacggccat gatcctgctg ccctacacct gggccgccgg
     1501 ctacgcgatg aagatcgcgc tgcggggcga cggttatggc gtggccgatg gccgcttgcg
     1561 ccagcgcgac gccgccgtgt cggcgctggc cttgctgtac gcggtgtggc tggtgtatgc
     1621 gggcggggtc aaatacctgc tgctgggcgc cttgctgtac gcgccgggcg ccgtgctgta
     1681 tgcgtgggcg cggcgcgagc acggcaagcg cgttttctcg cggcgcgaat ggtggggctt
     1741 tgcgggcctg ctgctggcgg ccggcgtggc ggcgctgggt ttgcagcagc ggtggctgag
     1801 tttgtgagtt aaaaatggct ggagcgcttg aagggcttgc gcgagcagct atgaaatgcg
     1861 tagtaatgaa aggtcaatga ccatgtcttc aaccgactcc tggcgcgtgg gcgacctgca
     1921 actcaccagc cgtttcctgc tcggcaccgc cggttacccg tcgccgcagg tgctggccga
     1981 cgcgattgcc gcttcgggca cgcaaatcgt cactgtgggc ttgaagcgcc agctcgccgc
     2041 cgccggcgac aacggctttg tcgacatcat ccgcagcgcg atgaaaacgc acagcgcgcg
     2101 cctgctgccc aacaccgccg gctgcaccac ggcgcgcgag gcgatccaac tggcccacat
     2161 ggcgcgcgag ctgtacggca cgccctgggt caagctggag gtggtgggcg acgaatacac
     2221 gttgcagccc gatccggtcg aactggtcga ggcggcacgg cagttgtcga aggacggctt
     2281 tgtcgtcttc ccctactgca ccgacgacct ggtgacgtgc aaacgcctgc tggacgccgg
     2341 ctgcccgctg ctcatgccgt ggggcgcgcc gattggttcg gggcaaggtt tggtcaaccc
     2401 cttcgcgctg cgcctgctgc gcgagcgcct gtccgatgtg ccgttgatca tcgacgccgg
     2461 catcggcgcg cccagccatg cggcccaggc gctggagatg ggttttgatg ccgtgctgct
     2521 caacagcgcc gtcagccaag ccagcgatcc ggtccgcatg gcggcggcgt tcaaggcggc
     2581 catcgaagcg ggtcgcaccg cgcggctggc gggtgtgatg gccaagcagg attttgcggt
     2641 ggcgaccacg ccggtcgcgg gcaatccgtt cttgatcaat tgataaaaat ggtgccagtg
     2701 cttgatccac ctgcgccagc agctcctgat gtggtagcaa aacccatcat ctggagcatt
     2761 gccggcaacg acagcggcgg cggcgcgggc ctcagcgccg accagcgcgc ggccgaggcg
     2821 tttggcgtgc acctgtgccc ggtggtggcg gcgatcaccg cgcagagctc ggtgggcgtg
     2881 gcgcgggtcg aggcggtgtc gcccgaagtg ctcgatgcgc aactggccgc gctggccgat
     2941 gacatgccgc ccgccgccat caagacgggc ttgctcggca gcgcgggcac cgtggccgtg
     3001 gtggcgcgct gggtcgacaa gctgcgcgcg cgtgggccgg tcgcgctggt ggtcgatccg
     3061 gtgctgggcg ccagcacggg cgcggcgttt gccgatgacg ccgtgctgcg tgcgtatcgc
     3121 gagttgctgc tgccgcgcgc gaccttgctg acgccgaacg agcgggaggc ggggcggctg
     3181 gtgggtgagg gcggtggcga ggatgcgccg cagcccctca ccccagccct ctccccaaac
     3241 gggcgaggga gtgaacagcc gggctcattc caaagcgacg cagcagtgtt gccccatctc
     3301 cccgctgggg agagggctgg ggtgaggggc acggcgcacc cagcacccgt cgcggttgtc
     3361 cccgccctgg cccaagccct gcgcgcacaa ggcacccaag ccgtcgccat caccggcggc
     3421 gacagcgcac cggcgccagg ctggtcgctc gactggattg acacccccca cgccagcggc
     3481 tggctcgccc tgccgcgcat cgacacgcgc cacacacacg gtaccggctg caccttcgcc
     3541 accagtgcgg cgtgcgcgat ggcgctgggc tttgtggccg ccgatgcgct ggtgctggcg
     3601 aagatggcca ccacgcacgc gctgcgtcat ggctacgcgg cggggcaggg cgcggggccg
     3661 gtggcggcgc gcggcgggtt tgccaacgat cccgcgctga tgccgatcat gtcgtggggc
     3721 gatgactctg attttgatag ctgcctgcgc ttgatagata agcggcagag cgcgatttcg
     3781 tccaaaaacg ccaatctcga cctgtacggc atagtcgaca gcgccgagcg cgtgcaacaa
     3841 gtagtgcagg ccggcgtgaa gctggtgcag ctgcgcatca agacccctga cgcgcccg
//
LOCUS       DAOMVO010000232         3891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6768_length_3891_cov_19.177007, whole genome shotgun sequence.
ACCESSION   DAOMVO010000232 DAOMVO010000000
VERSION     DAOMVO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6768_length_3891_cov_19.177007"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKV30_10485"
     CDS             <1..660
                     /locus_tag="PKV30_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HRL30670.1"
                     /translation="ITEEDIAQFLIHTPDFFERHADLLGAVQLTSPHGGRAVSLQERQ
                     AEMLREKIKLLEHRLMEMMRHGNENMLIADKLLRWSRQLFMVARPVDLPAVLVSEMKA
                     QFSVPQAALKLWDVAEVYADEPFAQGASDDVKSLAGSLTTPYCGVNAGFDAVQWLDDP
                     EAAASLTLIPLRAGAASPAFGLLVLASPDSQRYQAGMATDFLERIGELASAALSRVRD
                     T"
     gene            741..>1684
                     /locus_tag="PKV30_10490"
     CDS             741..>1684
                     /locus_tag="PKV30_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRL30671.1"
                     /translation="MRAEPSTVDTTQTALTERYLEHARVQKRLAPRTLALYSEDLHKL
                     MTAAAQAGIALTAVQNHHVRRWVAQMHAGGRSGRGIALILSGWRGFYTWLGREGQISA
                     NPVQDVRAPRQARPLPKALGVDEAVQLADHVAEDDDPWLEARDAAMVELLYGSGLRVG
                     ELVGLDVAASDAAQRAGRGWIDLPSAEVQVQGKGSKRRGVPLGTQALAALQRWLAVRD
                     QVAGEGDAAMALFIGRRGTRLTPQSIWQRLKRRSLLAGLATPVHPHMLRHSFASHVLQ
                     SSSDLRAVQELLGHAHIGTTQVYTRLDFQHLAKAYDAA"
     assembly_gap    1685..1784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1869..3596)
                     /locus_tag="PKV30_10495"
     CDS             complement(1869..3596)
                     /locus_tag="PKV30_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HRL30672.1"
                     /translation="MAWARWLRAWCLLLAAACLGGWFVPTAARALTPLTLDGSSQFPA
                     RLGAGGEFLLDPGGRATIEQVAAQSSFFFWRPTQLDRVYPVGDGQALWIRFVLAPRSE
                     REHWYLEIPFANLDRATLYARGADGTWQSRTAGDHVPVVDWPVPGRQPVLPLTLSSTQ
                     PTEHLLRIEHAFATSVPMMLTNEHQVLMRERVVTMGLGIYFGITLLGCVMALAAAVWM
                     RDLASALFVPPTLLLGLSAASFAGINGWLLWPRHAAWNDLSAFALPTLALVTMLMFVS
                     VATAFGTRAPRARWWPWALAGAGVAVTAALPFLSDAASARATAVVCTALLVGCIAVPA
                     WAWWRGGDRHALGLLIGMVCLAGPATTHVLRLVSVMPTGLISRFVLLSGAALQLAAVM
                     VTLLWRGRDRSLTRQRMRGLDRADPARGLATQAVVREQLRRMTARAQRQEHAYAVLLI
                     DLVNLAEVRHKFGRRAWQELPLRLADRLLANMREVDTVGRLSDTRFVMLMDGPVTADT
                     ASRLAQRILAQCLRPIRGRPEGWTPRVRMALGVLPRDGQDPRGVLDQLAIMLDTVAPG
                     DSRALFQRV"
     gene            3783..>3891
                     /locus_tag="PKV30_10500"
     CDS             3783..>3891
                     /locus_tag="PKV30_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL30673.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLTEAHGQKIAVI"
BASE COUNT          630 a         1339 c         1332 g          490 t
ORIGIN      
        1 atcaccgaag aagacatcgc ccagttcctg atccacacgc cggatttctt cgagcgccat
       61 gccgacctgc tgggcgccgt gcagctcacc agcccgcacg gcggccgtgc cgtgagcctg
      121 caggagcgcc aggccgagat gctgcgcgag aagatcaagc tgctggagca tcgcctgatg
      181 gagatgatgc gccacggcaa cgagaacatg ctgatcgccg acaagctgct gcgctggtcg
      241 cgccagctgt tcatggtggc gcgcccggtc gatttgccgg ccgtgctggt gagcgagatg
      301 aaggcgcagt tcagcgtgcc gcaggcggcg ctgaagttgt gggacgtggc cgaggtctac
      361 gccgacgagc cctttgcgca aggcgccagc gacgacgtga aaagcctggc cggctcgctc
      421 accacgccgt actgcggcgt caacgccggc tttgacgccg tgcagtggct ggacgacccg
      481 gaagccgccg cgtcgctcac gctgattccg ctgcgcgccg gcgccgccag cccggcattc
      541 ggcctgctgg tgctggcctc gcccgacagc cagcgctacc aggccggcat ggcgaccgat
      601 tttctcgaac gcatcggcga gcttgccagc gcggcgctgt cgcgcgtgcg ggacacctga
      661 cggcgccgcg ccaacgcttt tcaatgattt gagccgctgg cgcttgcagg acaagcgcga
      721 gcagctatca ttgcaatagc atgcgagcag agccatccac cgttgacacc acccagaccg
      781 cgctgaccga gcgctacctg gagcacgcgc gggtgcaaaa acgcctggcg ccgcgcacgc
      841 tggcgctgta cagcgaagac ctgcacaagc tgatgacggc agccgcccag gccggcatcg
      901 cgctgacggc ggtgcagaac caccacgtgc gccgctgggt ggcgcaaatg cacgccggcg
      961 ggcgcagcgg gcgcggcatc gcgctcatcc tttcgggctg gcgcggcttc tacacctggc
     1021 tcgggcgcga aggccagatc agcgccaacc cggtgcagga cgtgcgcgca cccaggcagg
     1081 cgcgcccgct gcccaaggcg ctgggcgtcg acgaagcggt gcagctggcc gatcacgtgg
     1141 ccgaagacga tgacccgtgg ttggaagcgc gcgacgccgc catggtcgag ctgctctacg
     1201 gcagcggcct gcgcgtgggc gaactggtcg gcctggacgt ggcggccagc gacgccgcac
     1261 agcgcgccgg gcgtggctgg atcgacctgc cgtcggccga ggtgcaggtg cagggcaagg
     1321 gcagcaagcg gcgcggcgtg ccgctgggca ctcaggcgct ggcggcgctg cagcgctggc
     1381 tggccgtgcg cgatcaggtg gcgggcgagg gtgatgcggc catggcgctg ttcatcggcc
     1441 ggcgcggcac acgcttgacg ccgcaatcga tctggcagcg cctgaagcgc cgcagcctgc
     1501 tggctggtct cgcgacgccg gtgcacccgc acatgctgcg ccattcgttt gccagccacg
     1561 tgctgcagtc cagcagcgac ctgcgcgcgg tgcaagagct gctgggccac gcgcacatcg
     1621 gcaccacgca ggtctacacg cggctggatt ttcagcatct ggccaaggcc tacgacgcgg
     1681 cgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaagat agctgccggc
     1801 gctgatctga cgtgcgccag cgggctaaaa agcctgaaaa atatcggctg gccgccgcgc
     1861 aacacgcgct acacgcgctg aaacagcgcg cggctgtcgc ccggcgcgac ggtgtccagc
     1921 atgatcgcca gctgatcgag caccccccgc gggtcttgcc cgtcgcgcgg cagcacgccc
     1981 agggccatgc gcacgcgcgg cgtccagcct tcggggcggc cacggatcgg gcgcaggcat
     2041 tgcgccagga tccgctgcgc cagccggctg gcggtgtcgg cggtgaccgg gccgtccatc
     2101 aacatcacga agcgtgtgtc gctcaggcgg ccgacggtgt cgacctcgcg catgttggcc
     2161 agcaggcggt cggccaggcg cagcggcagt tcctgccagg cgcggcggcc gaacttgtgc
     2221 cgcacttcgg ccaggttcac caggtcgatc agcagcacgg cgtaggcgtg ctcctggcgc
     2281 tgggcgcgcg ccgtcatgcg gcgcaactgc tcgcgcacca cggcctgcgt ggccaggccc
     2341 ctggccgggt cggcgcggtc caggccgcgc atgcgctggc gcgtcaggct gcggtcgcgc
     2401 ccgcgccaga gcagcgtcac catcaccgcc gccagttgca aggcggcgcc cgacaacaac
     2461 acgaagcgcg agatcagccc ggtcggcatg accgagacca ggcgcagcac gtgggtggtg
     2521 gccggcccgg ccaagcacac catgccgatc agcaagccca gcgcatggcg gtcgccgccg
     2581 cgccaccagg cccacgcggg caccgcgatg cagcccacca gcagcgccgt gcagacgacg
     2641 gcggtggcgc gcgccgaggc ggcgtcggac agaaacggca gcgccgcagt caccgccacg
     2701 ccggcgccgg ccaaggccca aggccaccag cgcgcacgcg gcgcccgggt gccgaacgcc
     2761 gtggccacgc tgacaaacat cagcatggtc accagcgcca gcgtgggcag ggcaaaggcc
     2821 gacaggtcgt tccacgccgc gtggcgcggc cacagcagcc agccgttgat gccggcaaag
     2881 ctggcggccg agaggcccag cagcagcgtg ggcggcacga acagggcaga ggccagatcg
     2941 cgcatccaca ccgccgccgc cagcgccatc acgcagccca gcagggtgat gccgaagtag
     3001 atgcccaggc ccatggtcac cacgcgctcg cgcatcagca cctggtgctc gttggtcagc
     3061 atcatcggca ccgaggtggc aaaggcgtgc tcgatgcgca gcaggtgctc ggtcggctgc
     3121 gtgctggaca gtgtcagcgg cagcaccggc tggcgcccgg gcaccggcca gtcgaccacc
     3181 ggcacgtggt cgccagcggt gcgcgattgc caggtgccgt cggcgccgcg ggcgtacagc
     3241 gtggcgcggt ccaggttggc gaaagggatc tcgaggtacc agtgctcgcg ctctgatcgc
     3301 ggcgccagca cgaagcggat ccacagcgcc tggccatcgc ccaccgggta aacgcggtcg
     3361 agctgggtcg gtcgccagaa gaagaaggac gattgcgcgg ccacctgctc gatggtggcg
     3421 cgaccgccgg ggtcgagcag aaactcgccg ccggcgccca gccgggccgg aaactgcgag
     3481 ctgccgtcca gcgtgagcgg cgtgagcgcg cgcgccgcgg tcggcacaaa ccagccgccc
     3541 aggcaagccg ccgccagcag caagcaccac gcacgcagcc accgagccca tgccatggtc
     3601 ggcggctgcg ctgcgtttgt tctccctgac atcgacgtgg acagcaccgg gccgagtaca
     3661 tcacgagcag cacgccagat gccaggcatt ccgtcacgct gaccgccggc aatggtgagg
     3721 gatacactgc ccttttttca cgctgtttcg cgccggccac ccggtccgcg cgcattttcg
     3781 acatgtcact gattcccgcc accatcctca cgggctttct cggctcgggc aagaccacgc
     3841 tgctcaagcg cgtgctgacc gaggcgcacg gccagaagat cgccgtgatc g
//
LOCUS       DAOMVO010000233         3860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6849_length_3860_cov_14.928252, whole genome shotgun sequence.
ACCESSION   DAOMVO010000233 DAOMVO010000000
VERSION     DAOMVO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6849_length_3860_cov_14.928252"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PKV30_10505"
     CDS             complement(<1..162)
                     /locus_tag="PKV30_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL30674.1"
                     /translation="MILRHTFTPLNNTRLANLCGPTDEHLRSIEAGLQIGIAHRHEQF
                     KIDGPKKQAE"
     gene            199..2562
                     /locus_tag="PKV30_10510"
     CDS             199..2562
                     /locus_tag="PKV30_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL30675.1"
                     /translation="MSTVTPVAPSMPAAIDSGPASLALPEAERRRVPPPRASAMASAV
                     DWLRRNALYVALLLTLASSLVCLVLSITQPAVGWDLRPVAGAAPTAPVGLRIHGVVPQ
                     GRADTAGLEAGMVVLALGVPGNDPVTLTAQDKLEDPDLLHNYSEWAAFYARQDRLARI
                     MKAPLVEATVVRPGHPPERVTLQPRAYRLVEGLPWSFWGQLLIGALAIGAAGWVVSVY
                     PSSPTTLFTAMSSFFVMLAGHSAAVFSSRELALPSGLFHALSVVNHASSALSLAALAL
                     LIGSYPKRLPRWPAWLEVALLPLLLAWIVADAAWLLPSPNWGSRSLQSLLMALVLINL
                     LRQGLAARGQPRVLRAVGGTALLLLCGTTMLILLLELLPIWRIMPPASQALIYAFVLV
                     SNLGIALATRRLRPYDLDDWTSQAFVALAGAVGVWVLYRGLVTLRVMEDSVALIVALL
                     VCWLLYVPLFRWLSRSYLKRAQPSLEDLTGEILLMGLIRPADRLAAWRGLLARLFNCR
                     CLDADAVPQAVADPARATLLPGGEVLWIPATAGMPALLLRDRVHARRRFNEHDRRLAQ
                     RLCGLVGQLVATRVAYGQGAEDERLRIADDLHDDLGAKLLSLVHASGRPDSNVEVPRM
                     AREALEEMRLSVRHLKAQRAPLTDVLADWRAETVARLSAAGIDVDWDAQLPERPRLLS
                     ARTSSQLTRVLREAVSNLIRHSDATHCRVLLNVSETELQLELEDNGRGMDQVAVAVSV
                     GHGLGNIERRVRRLGGSHRFVVGALGGTMLLMRVPLEPHAALSVAET"
     gene            2683..3339
                     /locus_tag="PKV30_10515"
     CDS             2683..3339
                     /locus_tag="PKV30_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL30676.1"
                     /translation="MNALLVVEDLPDIRAWLVDTARIAFPDSEVVAVGRCREGLGEAV
                     ARRFDVALIDLELPDGSGVAVIQALRTHQPQAMSVVLTIHDDDDHLFPALQAGAFGYL
                     LKESPQEDLVAQLRRIAQGEPPLSPPIARRVLNYFTSGGPRGLELSRFDGEEVALNAR
                     ETDILQRVAKGYTLPEIAGQLGLSRHTVADYVKQIYRKLNVSSRAEAALEAARRGLVK
                     "
     gene            3589..>3860
                     /locus_tag="PKV30_10520"
     CDS             3589..>3860
                     /locus_tag="PKV30_10520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HRL30677.1"
                     /translation="MIGRLAGQLAEKNPPQILIDCHGVGYEVWVPMSTFYNLPALGEK
                     LVLLTHFVVREDAQLLYGFGSEAERAAFRELIKISGIGPRMALAVLS"
BASE COUNT          504 a         1285 c         1395 g          676 t
ORIGIN      
        1 ctcggcctgc ttcttggggc cgtcgatctt gaactgctcg tgccggtgcg cgatgccgat
       61 ttgcagcccg gcttcgatgc tgcgcaggtg ttcatcggta ggaccgcaca gattggccag
      121 ccgcgtgttg ttcaaagggg tgaaggtatg ccggagaatc acgcagccat tgtcccccaa
      181 tcgtcagccg ccccgcgcgt gagtaccgtc acccccgtcg cccccagcat gccggccgcc
      241 atcgacagcg gcccggcctc gctggcgctg cccgaggccg agcggcgccg cgtgccaccg
      301 ccgcgcgcgt cggccatggc gtcggcggtc gactggctgc gccgcaacgc gctttacgtg
      361 gcgctgctgc tgacgctggc gtccagcctg gtctgcctgg tgctgagcat cacgcagccg
      421 gcggtgggct gggatttgcg gccggtggcg ggcgccgcgc ccaccgcccc cgtgggcttg
      481 aggattcacg gcgtcgtgcc gcaaggccgt gccgacacgg cgggcctgga ggcgggcatg
      541 gtggtgctgg cgctcggcgt gccgggcaac gatccggtta cgctgaccgc gcaggacaag
      601 ctggaagacc ccgatctgct gcacaactac agcgaatggg cagccttcta cgcgcggcag
      661 gatcggctgg cccgcatcat gaaggcaccg ttggtcgaag ccaccgtggt tcgcccgggt
      721 catccgcccg agcgcgtgac cttgcagccg cgcgcctatc ggctggtgga ggggttgccg
      781 tggtcgttct ggggccagtt gctgatcggc gcgctggcga ttggcgccgc cggctgggtg
      841 gtgagcgtgt accccagttc gcccaccacg ctgttcacgg cgatgtccag ctttttcgtc
      901 atgctggcgg ggcactcggc ggcggtgttc agcagccgcg agctggcctt gccgagcggg
      961 ctgtttcatg cgctgtcggt ggtcaatcac gccagctcgg cgctctcgct ggcggccttg
     1021 gcgttgttga tcggcagcta ccccaagcgc ctgccgcgct ggcctgcgtg gctggaggtg
     1081 gcgctgctgc cgctcctgct ggcgtggatc gtggccgacg ccgcctggct gctgccttcg
     1141 cccaactggg gcagccggtc gctgcagtcg ctgctcatgg cgctggtgct catcaatctg
     1201 ctgcggcagg ggctggcggc gcgtggccag cctcgggtgc tgcgcgcggt ggggggcacg
     1261 gcgctgctgc tgctgtgcgg caccaccatg ctgatcctgc tgctggagct gctgcccatc
     1321 tggcgcatca tgcctccggc gtcgcaggcg ctgatctatg ccttcgtgct ggtgtccaac
     1381 ctgggcattg ccttggccac gcggcgcttg cggccgtacg acctggacga ctggacttca
     1441 caggctttcg tggcgctggc gggcgcggtg ggtgtgtggg tgctgtaccg cggcctcgtc
     1501 acgctgcgcg tgatggagga ttcggtggcg ctgatcgtgg ccctgctggt gtgctggctg
     1561 ctctacgtgc ccttgttccg ctggctcagc cgctcttacc tgaagcgcgc ccagcccagc
     1621 ctggaagacc tgaccggtga aattctgttg atgggcctga ttcgcccggc ggatcgcctg
     1681 gccgcctggc gcgggctgct ggcgcgcctg ttcaactgcc gctgcctgga cgccgatgcc
     1741 gtgccgcagg ccgtcgcaga cccggcgcga gccacgctgc tgccgggtgg cgaggtgctg
     1801 tggattcccg ccacggccgg catgccggcg ctgctgctgc gcgaccgggt gcacgcccga
     1861 cgccgcttca acgagcacga ccgccgcctg gcgcagcgct tgtgcggcct ggtggggcag
     1921 ctggtggcca cccgcgtggc ctacgggcag ggcgccgagg acgagcgcct gcgcattgcg
     1981 gacgacttgc acgacgacct gggcgccaag ctgctgtcgc tggtgcacgc cagcgggcgg
     2041 ccggattcga acgtcgaggt gccgcgcatg gcgcgcgagg cactggagga aatgcgcctg
     2101 tcggtgcgcc acctcaaggc ccagcgggcg ccactgaccg acgtgctggc cgactggcgc
     2161 gccgaaaccg tggcgcgcct gagcgccgcc ggcatcgacg tcgattggga tgcccagttg
     2221 cccgagcggc cacggctgct gtcggcacgc acctcgtcgc agctgacgcg cgtgttgcgc
     2281 gaagccgtgt ccaacctgat tcgccacagc gatgccacgc actgccgcgt gctgctgaac
     2341 gtgagcgaga ccgagctgca actggagctg gaggacaacg gccgcggcat ggaccaggtg
     2401 gcggtggccg tgtcggtcgg ccacgggctg ggcaacatcg aacggcgcgt gcggcgcctg
     2461 ggcggatcgc accgtttcgt ggtgggggcg ctgggcggaa cgatgctgct catgcgcgtg
     2521 ccgctggagc cgcacgccgc cttgtcggtg gctgaaacct gaggcgccga ccggcctgcg
     2581 tgcactggcg gccacgccgg acggacatat ttggcgcccg ggcgcttcgc gagaggggcc
     2641 agcgccgcgc cgcggccacg gcttgcttag catgctcagt ctatgaacgc attgctcgtc
     2701 gtcgaggatc tgccggacat ccgcgcctgg ctggtcgaca ccgcccgcat cgcctttccg
     2761 gacagcgagg tggtggccgt ggggcgttgc cgcgaaggac tgggcgaggc ggtcgcgcgc
     2821 cgcttcgacg tggccctgat cgatctggaa ttgccggacg gcagcggcgt cgccgtgatc
     2881 caggccctgc gcacccacca gccgcaagcc atgtcggtgg tgttgaccat tcacgacgac
     2941 gacgatcacc tgtttcccgc gctgcaggct ggcgccttcg gctatctgct gaaggagtcg
     3001 ccgcaggaag acctggtggc gcaactgcgg cgcatcgccc agggcgagcc gccgctgtcg
     3061 ccgcccatcg cgcggcgggt gctgaactac ttcacgagtg gcggcccgcg cggcctggaa
     3121 ttgagccgat tcgacggcga agaagtggcg ctcaatgcgc gcgaaaccga catcctgcag
     3181 cgcgtggcca agggctacac gctgcccgaa atcgccggcc agctcggtct gtcgcgccac
     3241 acggtggccg attatgtgaa gcagatctac cgcaagctca acgtgtcgtc acgcgccgag
     3301 gcggcgctgg aggcggcgcg gcgcgggttg gtcaagtagc cccctgagtc gccttcggcg
     3361 ccttcccccc agggggacaa cgctggtggg cggggggacc ccgcccacgg cgttcccgag
     3421 cggcctgctc cgcggccgtc cgaaaggcct gctgcgcggc ctgagctgct tatttgctac
     3481 aaaaatagaa gctgctcgcg cttgccagtc aagcgttgga gggtgattta tcattgaaac
     3541 tgcacggcgt ttggcgccgt cgttccttgt ctccctcgaa agcacgcctt gatcggacgc
     3601 cttgctggcc agttggccga gaagaacccg ccgcaaatcc tgatcgactg ccatggcgtc
     3661 ggctacgagg tgtgggtgcc gatgagcact ttctacaacc tgccggcgct gggcgagaaa
     3721 ctcgtgctgc tgacgcactt cgtggtgcgc gaggacgcgc agctgttgta cggtttcggc
     3781 agcgaggccg agcgcgcggc gtttcgtgaa ctgatcaaga tcagcggcat cggcccgcgc
     3841 atggcgctgg cggtgctgtc
//
LOCUS       DAOMVO010000234         3856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6861_length_3856_cov_20.925546, whole genome shotgun sequence.
ACCESSION   DAOMVO010000234 DAOMVO010000000
VERSION     DAOMVO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6861_length_3856_cov_20.925546"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..540)
                     /locus_tag="PKV30_10525"
     CDS             complement(109..540)
                     /locus_tag="PKV30_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30678.1"
                     /translation="MRWMLLIVAVVAGFYGWKHTGERAVAREVAHVAVRNPSGFVPVA
                     MPADAPARTVWVFAPQNCPSDAAARADNLARTLQAEGIPVVRASSLSIQITAPDEETK
                     QRLQRLDVVMRGTIPAVLIDGMGKANPSAEEVIREFRRTGG"
     gene            801..>3856
                     /locus_tag="PKV30_10530"
     CDS             801..>3856
                     /locus_tag="PKV30_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRL30679.1"
                     /translation="MWRASVGLWLAFMALVTVLWPMAARAQDADCAEVKIVIEQKLSL
                     ERQAFDAHMIIRNGLDGQLTNVKVELFYQDQNQQPVVATSDPNAVGATFFQRVDRMTG
                     INSLDGGSLAGKTNADIHWLIIPSQGAGGDTANGRMYYIGAKVTYTLAGEATTVDVTP
                     DYVVVRPQPLLVLDYFLPTDVYADDAMTAEVEPTEPFTLGVRVANVGAGVSAKTTIES
                     AQPKIVENRQGLLIDFRILGGYVGNDMLGKSLLLDFGDIPGQRAKVGRWIMETSLAGR
                     FVEFNASFTHADSLGGAVTSLIKEVRTHKLVHDVLVDLAGHDDVYDFLAEFGNGYWVY
                     DSSGGDAEVVDVSKQASLTNVSNGNLRLAFPASSNLIHVKLPDPFSGAKPITRVVRSD
                     GKVLPTQNYWLSKTRNADLSWSYFLHVFDSNSTGDYILEFGQGTNGSIAGTAYRDSNA
                     NGIRDSGEPAEGNLGIVLKGVDANGQSILRQGYTDPSGAFSFTGLAPGRYQLEAAVVD
                     GWIDGTWMAGSAGGAAQPGLIKDIVLTAGTTGTGYLIAKRKPDANPNTDKADVSIAIQ
                     ATKSQLRGGEATSVTVTVRNAGESSAQGVTAQVAVPAGLTLQGNNASLGSYAGGAWTI
                     GGLTKGQSATLALDVKADQVSGTKDKTLSWPVSVSASTTDPQTGNNSALLGLTVLADK
                     TNTVEMSQTLPAEARVLMLVSCPQAAAAEQSTCETAAVQSAHDTLAGNVQALQTAATL
                     SGWNVAQRSGAYNVLWLHGGADKLDDQALAEIRAAVRRGATLIVDGLPGVAGSEPKLN
                     QLADVMSARIALPAIGANQSVKFPNEPTAQPGGGTLYGLQLQSPPAQRLADSASSGAA
                     VIASSTWGHGQSWVMGFDLLASLQGPAATFWGGYASQQLKAFTPVSRSDPALAGARLP
                     LRTTVRSNAEAGSTPQDVSVRVQLPSGLAHSDVAPTPVRDESQLVEWAWRLAPAQSAT
                     GDMRLTLPQTSGTLQVQTTLLDSAATSLDLQTQAITVVGLDSLTPQV"
BASE COUNT          712 a         1396 c         1156 g          592 t
ORIGIN      
        1 cacaacattc gctgatcact ccctccgcgg taaccggacg cgaggcagac aagctgagct
       61 gggcagtcac cacgcaactg accgcgcgct gccggcaaat caggttcttc accctcccgt
      121 gcgccggaac tccctgatta cttcctcggc cgacggattt gccttgccca tgccatcgat
      181 cagcacagct ggaatcgtcc cgcgcatcac cacgtcgagc cgttgcaggc gttgctttgt
      241 ctcttcgtcg ggcgccgtga tctgaatcga cagtgacgat gcgcgcacca ccggaattcc
      301 ctctgcttgc agcgtccgcg ccaagttatc tgcccgcgcc gccgcatcgg aagggcagtt
      361 ctgcggcgca aacacccaca cggtgcgtgc cggcgcatcg gccggcatcg cgaccggcac
      421 gaagccgctc ggattgcgca cggccacatg cgccacctcc cgcgccacag cgcgctcgcc
      481 cgtgtgcttc cacccgtaaa accccgccac cacggccacg atcaaaagca tccagcgcat
      541 cgtcttattc tgaacgattg cttacgcgac atgcagcgcc acagccacat tgcgaggttc
      601 atatccctct aatttggtag gaaagtaatt gttccgccgt aaggccatgg gtatagttct
      661 atcagtatgt acgcggccgc tgacctgtgg aagggttggt caggcaccta taaaacaagc
      721 gttgacctgg cgccaagctg ggcaccccgg gacgaggaag agggcgtatg cgaggacgtc
      781 gagcgagggg aagcgcgcag gtgtggcgcg cgtcggtggg gctgtggctg gccttcatgg
      841 cgctggtgac cgtgctgtgg ccgatggctg cgcgcgcgca ggacgccgac tgcgccgaag
      901 tcaagatcgt catcgagcaa aagctctccc tggagcgtca agccttcgac gcccacatga
      961 tcatccgcaa cggcctggac ggccagctca ccaacgtcaa ggtcgagctg ttctaccagg
     1021 accagaacca gcagccggtg gtcgccacca gcgatcccaa cgccgtcggc gccaccttct
     1081 ttcagcgcgt agaccgcatg acggggatca actcgctgga cggcggcagt ctggctggca
     1141 agaccaatgc cgacatccac tggctgatca ttccctcgca gggtgcgggt ggcgacaccg
     1201 ccaacggccg catgtactac atcggcgcca aggtcaccta caccctggct ggtgaggcca
     1261 ccaccgtcga cgtcaccccc gactacgtcg tggtgcgtcc gcagccgctg ctggtgctgg
     1321 attacttcct gcccaccgac gtctacgccg acgacgccat gaccgctgaa gtcgagccga
     1381 ccgagccctt caccctgggc gtgcgcgtcg ccaacgtcgg cgccggtgtc tcggctaaga
     1441 ccaccatcga atcggcccag cccaagatcg tcgagaaccg ccagggcctt ctgattgatt
     1501 tccgcatcct gggcggctac gtgggcaacg acatgctcgg caagagcctg ctgctggact
     1561 tcggcgacat cccgggccag cgcgccaagg tcgggcgctg gatcatggaa acctcgctcg
     1621 ctggccgctt cgtcgaattc aacgccagct tcacccacgc cgattcgctc ggcggcgccg
     1681 tcacctccct gatcaaggaa gtacgcacgc acaagctggt gcacgacgtg ctggtcgatc
     1741 tggccggcca tgacgacgtc tacgacttcc tggccgagtt cggaaacggc tattgggtct
     1801 acgactccag tggtggcgac gcggaagtgg tcgatgtctc caagcaagcc agcctgacca
     1861 atgtctccaa tggcaatctg cgcctcgcct tcccggcgtc ctccaacctg atccacgtca
     1921 agctgcctga cccctttagc ggcgcaaagc ccatcacccg cgtggtgcgc agcgacggca
     1981 aagtgctgcc gacgcagaac tattggctgt ccaagacccg caacgccgac ctgagctggt
     2041 cctacttcct acacgtcttc gacagcaact cgactggcga ctacattctg gaattcggcc
     2101 aaggcaccaa cggcagcatt gccggcaccg cctatcgcga cagcaacgcc aacggcatcc
     2161 gcgacagcgg cgagccggca gaaggcaacc tcggcatcgt gctcaagggc gtcgatgcca
     2221 atggccagag catcctgcgc cagggctaca ccgatccgtc tggcgccttc agcttcacgg
     2281 gtctggcacc cggccggtac caactcgaag ccgctgtcgt cgacggctgg atcgacggca
     2341 catggatggc tggcagcgct ggtggcgcag cccagcccgg cctgatcaag gacatcgtgc
     2401 tcaccgccgg caccaccggc accggctacc tgatcgccaa gcgcaagccc gacgccaacc
     2461 ccaataccga caaagcagac gtctcgatcg ccatccaggc caccaagtcg caactgcgtg
     2521 gtggtgaagc gaccagcgtc accgtcaccg tgcgcaacgc cggcgaatcc agcgcgcagg
     2581 gcgtcacggc ccaggtcgcg gtgcctgccg gcctgaccct tcagggcaac aacgccagcc
     2641 tcggcagcta cgccggtggc gcctggacca tcggcggcct caccaagggc cagagcgcca
     2701 ccctcgcgct cgacgtcaag gcggaccaag tcagcggcac caaggacaag accctctcct
     2761 ggccggtgag cgtcagcgcc agcaccaccg acccccagac cggcaacaac agcgccttgc
     2821 tcggcctcac cgtcctggcc gacaaaacca atactgtcga gatgtcgcaa acgctgcccg
     2881 ctgaagcgcg tgtgctcatg ctcgtgtcct gcccgcaagc cgccgcggcc gagcagtcca
     2941 cctgcgaaac cgcagctgtg cagtcagcac acgacaccct ggccggcaac gtccaagcgc
     3001 tgcaaacggc cgccacgctc tccggctgga acgtcgccca gcgcagcggc gcgtacaacg
     3061 tcctctggct gcatggcggc gcagacaagc ttgacgacca ggccctggct gaaatccgcg
     3121 ccgccgtccg ccgcggcgcc accctgatcg tcgatggcct gcctggcgtg gccggcagcg
     3181 agcccaagct caatcagttg gcggacgtga tgagcgcgcg catcgcgctg ccggccatcg
     3241 gcgccaacca gtcggtcaag ttccccaacg aacctaccgc ccagccgggg ggcggcacgc
     3301 tctacggtct gcaactgcaa tcgccgccgg cccagcgcct ggccgacagc gccagctcgg
     3361 gcgccgccgt gatcgccagc agcacctggg gccatggtca atcctgggtc atgggcttcg
     3421 atctgctggc cagcctgcaa ggcccggccg ccaccttctg gggcggttac gccagccagc
     3481 aactgaaggc gttcaccccg gtctcgcgca gcgacccggc tctggccggc gcgcgcctgc
     3541 cgctgcgcac cacggtgcgc agcaacgccg aggccggcag cacgccgcag gatgtgagcg
     3601 tgcgcgtgca actgccctcg ggcctggccc acagcgatgt ggcgccgacc ccggtgcgcg
     3661 acgagtcgca attggtggag tgggcctggc gtctggcgcc cgcccagagc gccacgggcg
     3721 acatgcgcct cacgctgcca cagacctcgg gcacgctgca ggtgcagacc acgctgctcg
     3781 acagcgccgc gacctcgctc gatctccaga cccaggccat caccgtggtc ggcctggaca
     3841 gcctgacgcc gcaggt
//
LOCUS       DAOMVO010000235         3853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6867_length_3853_cov_13.711690, whole genome shotgun sequence.
ACCESSION   DAOMVO010000235 DAOMVO010000000
VERSION     DAOMVO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6867_length_3853_cov_13.711690"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKV30_10535"
     CDS             complement(<1..583)
                     /locus_tag="PKV30_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30680.1"
                     /translation="MSEVLLSARNLTKKFGGLAAVNDVSVDLWRERIHCVIGPNGAGK
                     STLTNLLSGDLPPTSGTIALGTHDITSASPEQISRRGLGRSYQKTNIFLPFTVWENVR
                     LAAQSRAAQQPFNPLRWLQSARGDAAVNERAEKALELAGLTARRDFIAGAASHGEQRQ
                     LEIAMTLATDPQVLLLDEPLAGMGVAEAERMVEL"
     gene            complement(576..1604)
                     /locus_tag="PKV30_10540"
     CDS             complement(576..1604)
                     /locus_tag="PKV30_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL30681.1"
                     /translation="MSAPQSNLETLPRSIRVVLALGLVALIAFPFVGSEFYQQMVARM
                     MILAIFAMSLDLLQGVTGLVSLGHAAYFGLGGYALALLSSDNGPVSLWWTLPAAVLVA
                     GLFALVIGFFVVRTRGIYFIMVTMAFAQMLFFLVFDNKELNVFGLFPLKLGGSDGVYI
                     NFKPDAGLFDLENKRVFYYFVLACLVLTYVFLRRVLWSPFGRALAGIRVNEHRMRAVG
                     FDSFGYKLTAFTLAGALAGLAGYLWGAQTGFVNPELMGFHMSAHAIMMVILGGMGNFA
                     GAIVGAFAFEFMLELFKDLPAIGDFNTGKHWQLWMGLFIVAVIVFAPRGLLGLLARFT
                     GRKGGEHE"
     gene            complement(1601..2464)
                     /locus_tag="PKV30_10545"
     CDS             complement(1601..2464)
                     /locus_tag="PKV30_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL30682.1"
                     /translation="MDFANFLIQTLNSVQYGLLLFMLAAGLTLIFGIMGVVNLAHGSF
                     YMLGAYLAFSLSSRLGSFTAAIIVGTLLSVVFGLVLERLLFRYFYHRDHLDQVLLTFG
                     LIYIFEEMRSLFWGDDVHGVAIPPLLKGSIALTENQSYPVYRLFMMGVCVLLAIGLYL
                     LINKTRLGMKIRAGAFNREMTESLGVNIKLIHSVVFAIGVALASVAGMIAAPVSSVYP
                     GMGSSVLIMCFVVVVIGGIGSVRGALIAALLVGFVDTFGKVLLPQVAGMLVYMLMAAV
                     LLWKPEGLFKQ"
     gene            complement(2655..3800)
                     /locus_tag="PKV30_10550"
     CDS             complement(2655..3800)
                     /locus_tag="PKV30_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30683.1"
                     /translation="MGASSAGLLLPQIVRAQGNKVRVGFMLPYTGTYAQLGVAIENGV
                     RMAIAEKGSKLGGREIEWFKVDDESEPSKGVENAQKLVQRDKVDVLIGTVHSGVQMGI
                     HKVARDSGVLNIIPNAGVLAATRALCAPNVFRASFTNSQPTMGLGKPMMDKGLKRAVW
                     ISWKYAAGDDALEGFKDSYTKAGGTIIKELSLPFPNVEFQALLTEIASLKPDAVACFF
                     AGAGAAKFMKDYAAAGLQGKIPLWGSGFLTEGVLEASGAAAEGVMTTLHYADSLDTPR
                     NKQFRTAYAGQFKLQPDVYAVQGYDSGQLLVQGLAAVKGDMSNKAALYKALETTVIDS
                     PRGKWTMSKAHNPIQDIYLRVVEKGDNKVLGVAAKALADSGAGCKMG"
BASE COUNT          747 a         1281 c         1216 g          609 t
ORIGIN      
        1 gcagctcgac catgcgctcg gcctcggcca cgcccatgcc ggccagcggc tcgtcaagca
       61 acagcacctg cggatcggtg gccagcgtca tggcgatttc cagctgacgc tgctcgccgt
      121 ggctcgcggc gccagcgatg aagtcgcgcc gggcagtcag gccggccagc tccagcgctt
      181 tttcagcgcg ctcgttcacg gcagcatcgc cgcgcgcgct ttgcagccag cgcagcgggt
      241 tgaacggctg ctgcgccgcg cgcgattgcg cggccaggcg cacgttttcc cacacggtga
      301 agggcagaaa gatgttggtc ttctggtagc tgcggccgag accgcggcgc gagatctgct
      361 cgggcgaggc gctggtgatg tcgtgcgtgc cgagggcaat ggtgccgctg gtcggcggca
      421 gatcgcccga cagcaggttg gtcagcgtcg acttgccggc gccgttgggg ccgatcacgc
      481 agtggatgcg ctcgcgccac agatcgaccg agacatcgtt gacggcggcg aggccgccga
      541 acttcttggt caggttcctg gcgctgagga gcacttcact catgctcgcc ccccttgcgc
      601 cccgtgaagc gcgccagcag gccgagcagc ccgcgcggcg cgaacacgat caccgccacg
      661 atgaacaagc ccatccacaa ctgccagtgc ttgccggtgt tgaagtcgcc gatggcgggc
      721 aagtccttga acagctccag catgaactcg aacgcaaagg cgccgacgat ggcgccggca
      781 aagttgccca tgccgcccag aatgaccatc atgatggcgt gcgcgctcat gtggaaaccc
      841 atcagctcgg ggttcacgaa acccgtctgc gcgccccaca gatagcccgc cagccccgcc
      901 agcgcgcccg ccagcgtgaa agccgtcagc ttgtagccaa aactgtcgaa gcccacggcg
      961 cgcatgcgat gctcgttgac gcggataccg gccagcgcgc ggccaaacgg gctccacagc
     1021 acgcggcgca aaaacacgta ggtcagcacc aggcaggcca gcacgaagta gtagaagacg
     1081 cgcttgttct ccagatcgaa caggccggcg tccggcttga agttgatgta gacgccatca
     1141 gagccgccca gtttcagcgg gaacaggccg aagacgttca gctccttgtt gtcaaacacc
     1201 agaaaaaaca gcatctgcgc gaacgccatc gtcaccatga tgaagtagat gccgcgcgtg
     1261 cgcaccacga aaaagccaat gaccagcgcg aacaacccgg ccaccagcac ggcggcgggc
     1321 agcgtccacc acaggctcac cggcccgttg tcggacgaca gcagcgccag cgcgtagccg
     1381 cccaggccga agtacgcggc atggccgagc gacaccaggc cggtgacgcc ctgcagcaga
     1441 tcgaggctca tggcgaagat ggccaggatc atcatgcgcg ccaccatctg ctggtagaac
     1501 tcgctgccga cgaaagggaa ggcgatgagc gcgactaggc ccagcgccag cacgacccgg
     1561 atgctgcgcg gcagggtttc gaggttggat tgcggcgcgc tcattgcttg aacaaacctt
     1621 caggcttcca gagaagaaca gcggccatca acatatagac cagcatgccc gccacctgcg
     1681 gcagcagcac cttgccgaag gtatcgacaa agcccaccag cagcgcggcg atgagcgcgc
     1741 cgcgcaccga gccgatgccg ccgatcacca ccaccacgaa gcacatgatc agcaccgacg
     1801 agcccatgcc cgggtacacg ctcgacacgg gcgccgcgat catccccgcc accgaggcga
     1861 gtgccacgcc gatggcgaac accaccgagt gaatcagctt gatgttgacg cccagcgact
     1921 ccgtcatctc gcggttgaag gcgccggcgc ggatcttcat gcccagtcgc gtcttgttga
     1981 tcagcaggta cagcccaatg gccagcagca cgcacacgcc catcatgaac aggcggtaga
     2041 cgggatacga ctggttttcg gtcagcgcga tggagccctt cagcagcggc ggaatcgcca
     2101 cgccgtgcac gtcgtcgccc caaaacagcg agcgcatttc ttcaaagatg tagatcagcc
     2161 cgaaggtgag cagcacctgg tccaggtgat cgcggtggta gaaatagcga aacagcaggc
     2221 gctccagcac cagaccgaac accaccgaca gcagcgtgcc cacgatgatc gccgccgtga
     2281 aactgccgag ccgcgacgag agcgaaaacg ccaggtacgc gcccagcatg tagaagctgc
     2341 cgtgcgccag gttgaccacg cccatgatcc cgaagatcag cgtcagcccg gccgccagca
     2401 tgaacagcag gagaccgtac tgcacgctgt tgagcgtctg gatcaagaag ttggcgaagt
     2461 ccacggtggg atcggatgaa atgcccggcg cctgggcggt tgaagaatga cgaaatgatc
     2521 tcgctgcgct tcgatgacgg cgctgcgcgc catgatcaag tcaagtcacg cgccgcaagg
     2581 cgcgtcatcg ccgcaggccg aggtcatgcg cccgataggg cgcggtcatg cattgcggcg
     2641 gcagagggtg ccagtcaacc catcttgcaa cctgcgcccg agtcggccag cgccttggcc
     2701 gccacaccca gcaccttgtt gtcgcctttt tcgaccacgc gcaggtagat gtcctgaatc
     2761 gggttgtggg ccttgctcat cgtccacttg ccgcgcgggc tgtcgatgac ggtggtttcc
     2821 agcgccttgt acagcgcggc cttgttcgac atgtcgccct tgacggcggc caggccctgc
     2881 accagcaact gccccgagtc gtagccctgc acggcgtaca cgtcgggctg cagcttgaac
     2941 tggcccgcat aggcggtgcg gaactgcttg ttgcgcggcg tgtcgagcga gtcggcgtaa
     3001 tgcagcgtgg tcatcacacc ctcagcggcg gcgccgctgg cttccagcac accttcggtc
     3061 aggaagcccg agccccacag cggaattttg ccctgcaggc cggcggcggc gtagtctttc
     3121 atgaacttgg cggcgccagc gccggcaaag aagcaggcca ccgcatcggg tttgagcgac
     3181 gcaatttcgg tcagcagcgc ctggaactcc acgttcggga agggcaggct caattccttg
     3241 atgatggtgc cgccggcctt ggtgtagctg tccttgaagc cttccagcgc gtcgtcgccg
     3301 gcggcgtatt tccagctgat ccagaccgcc cttttcaggc ccttgtccat catgggcttg
     3361 cccagaccca tggtgggctg gctgttggtg aagctggcgc ggaacacgtt gggcgcgcac
     3421 agcgcacgcg tggcggccag cacaccggcg ttggggatga tgttgagcac acccgaatcg
     3481 cgtgccacct tgtggatgcc catctgcacg cccgagtgca cggtgccgat cagcacgtcg
     3541 accttgtcgc gctgcaccag cttttgcgcg ttctccacgc ccttggatgg ctcggattcg
     3601 tcatcgacct tgaaccactc gatctcgcgc ccgcccagct tgctgccttt ttcggcaatc
     3661 gccatgcgca cgccgttttc aatcgccacg cccagctgtg cgtaagtgcc ggtgtagggc
     3721 agcatgaagc cgacgcgcac cttgttgcct tgcgcgcgca cgatttgcgg cagcagcagg
     3781 cccgcggaac tggcgcccac gacggcggcg ctgcgcgaga ggatcagacg gcggctcagg
     3841 ccgcgggaga cag
//
LOCUS       DAOMVO010000236         3851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6874_length_3851_cov_15.548736, whole genome shotgun sequence.
ACCESSION   DAOMVO010000236 DAOMVO010000000
VERSION     DAOMVO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6874_length_3851_cov_15.548736"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKV30_10555"
     CDS             <1..596
                     /locus_tag="PKV30_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRL30684.1"
                     /translation="RIGYHFIYPGVGYGGSCFPKDVKALIHTAQTIGFTPDVLLAVEQ
                     RNDAQRRVLADRIAQHYGGDMRGKTIAVWGLAFKPETDDMREAPSRALLQALWAAGAQ
                     VRAHDPVAMDEARRIFGERADLVLCDTPQQALQGADCLAIVTEWKTFRVPDFAQMARL
                     LKDRMVFDGRNLYEPAVLAQHGLGYESIGRPGIAPAP"
     gene            665..1513
                     /locus_tag="PKV30_10560"
     CDS             665..1513
                     /locus_tag="PKV30_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HRL30685.1"
                     /translation="MPRFAANLSLLYTELPFLDRFEAAARDGFEAVEYLFPYAFDLAE
                     LLARLKANGLQQVLFNAPPGGTDAPGIDAAWAAGARGTASVPGREAEFRAGVELALRY
                     ADALYCPRIHCMAGLLSESAAGADQESAARSVYVSNLRWAATEAAKSGRTILIEPINP
                     RDMPRFFLNRQDDAHAVVQEVGAPNLQVQMDLYHCQIVEGDVAMKLRQYLPTGRVGHL
                     QIAGVPERHEPDVGELNCAYLFEVIDEVAAQCGWQGWVGCEYRPRMGAEPGGTSRGLG
                     WRPRAG"
     gene            1619..2071
                     /locus_tag="PKV30_10565"
     CDS             1619..2071
                     /locus_tag="PKV30_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30686.1"
                     /translation="MSPPQPNRLTARIAMGLLAATLCAGSAWADKPEWAGRGNGNSQG
                     DGPPGQMKKQQRYDADRRHYDDGRYQRPDRHRRAPIAVKPGAYFSDGQRSNAHQWYSA
                     QYHAGHCPPGLAKKNNGCLPPGQAKKWRVGQPLPVGVVYHAVPQPVLI"
     gene            2226..2828
                     /locus_tag="PKV30_10570"
     CDS             2226..2828
                     /locus_tag="PKV30_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30687.1"
                     /translation="MTENLPMLNRRHCHRLLGAAALITLFPGLSACAAGSGGMPGYTV
                     SQQRLNELLARQFPVTRNLLGGLADLTLSSPRLGLLPASNRISTMFDLGLTERLAGSR
                     YSGGMDLDYGLRLDLQEGAVRMADVRVNRLTIDQLPRTQQQLVSQYAPDIAGRLLSDM
                     VLYRLPAEQLALARNMGWGAAAVRVLPDGLRIDMGQAGTR"
     gene            3104..>3851
                     /locus_tag="PKV30_10575"
     CDS             3104..>3851
                     /locus_tag="PKV30_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL30688.1"
                     /translation="MHNTHLRIGHGAHLVIALHGWFGHAGGWGPFTQQLNTQDFSYVF
                     MDQRGYGGMRRSGGPYTVEQIAQDALALADRLGWQRFSLIGHSMGGSSIQQVLAAAPE
                     RVRALVALTPVSAIGVPFDEQGWQLFSAAGSDVQARRAIIDFTTGNRLTGTWLDAMAA
                     STQTNSDDEAVAGYLQSWAKTSFVDKIQGQQLPVLVLPGEHDPALGEAACRATWMQHY
                     PNAQLEVVRNAGHYPMDETPIILATTIEKFL"
BASE COUNT          618 a         1386 c         1263 g          584 t
ORIGIN      
        1 cgcgcatcgg ctaccacttc atctaccccg gcgtgggcta cggcggcagc tgcttcccca
       61 aggacgtgaa ggcgctgatc cacaccgcgc aaaccatcgg cttcacgccc gatgtgctgc
      121 tggccgtgga gcagcgcaac gacgcccagc gccgcgtgtt ggccgatcgc atcgcgcagc
      181 actacggcgg cgacatgcgc ggtaagacga ttgcggtgtg gggcctcgcc ttcaagcccg
      241 agaccgacga catgcgcgag gcgcccagcc gcgcgctgct gcaggcgctg tgggccgccg
      301 gcgcgcaggt gcgcgcccac gaccccgtgg ccatggacga ggcgcgccgc atcttcggcg
      361 agcgcgccga cctggtgctg tgcgacacgc cgcagcaagc gctgcaaggc gccgactgcc
      421 tggccatcgt caccgaatgg aagaccttcc gcgtgcccga ctttgcgcaa atggcgcggc
      481 tgttgaaaga ccgcatggtg ttcgacggcc gcaacctgta cgagccggcg gtgctggcgc
      541 agcacgggct gggctatgaa tccattggtc gacctggcat cgcgccggca ccatgaaact
      601 atcaattcag tagctgcttg cgcttgatgg gcaagcgcta gcgcctggtt tcatataaat
      661 cccgatgcct cgctttgccg ccaacctgag cctgctgtac accgagctgc cctttctcga
      721 ccgcttcgag gccgcggcgc gcgacggctt tgaggcggtg gaatacctgt ttccctacgc
      781 gttcgatctc gctgagttgc tcgcccgctt gaaggcgaac gggctgcagc aagtgctgtt
      841 caacgccccg cccggcggca ccgacgcgcc gggcatcgac gccgcctggg ccgccggcgc
      901 acgcggcacc gccagcgtgc cggggcgcga agccgagttc cgcgccggcg ttgaactggc
      961 gctgcgctat gccgacgcgc tgtattgccc gcgcatccat tgcatggccg gtttgttgag
     1021 tgaatcggcc gctggcgcag atcaggaaag cgcggcacgc tctgtttatg tgagcaacct
     1081 gcgctgggct gcaaccgaag cggccaagtc tggccgcacc atcctgatcg agcccatcaa
     1141 cccgcgcgac atgccgcgct tcttcctcaa ccgccaggac gacgcgcacg ccgtcgtgca
     1201 ggaagtcggc gcgcccaacc tgcaggtgca gatggacctg taccactgcc agatcgtcga
     1261 gggcgacgtg gcgatgaagc tgcgccaata cctgcccacc ggccgcgtcg gccacctgca
     1321 gatcgccggc gtgccggagc ggcacgagcc cgacgtgggt gagctcaact gcgcctacct
     1381 gttcgaggtc atcgacgaag tggccgcgca atgcggctgg caaggctggg tcggctgcga
     1441 ataccggccg cgcatgggcg ccgagccggg cggcacatcg cgcgggctcg gctggcggcc
     1501 gcgtgcgggc tgacggcatc gcccgcacgc cgatgcgccc tgctcctaca gggccggcgc
     1561 gatgcgctgc ttaccctggt cgctgcaagg cgcgcccggc gcgcacgaaa ggacgaacat
     1621 gagcccccca caaccgaacc gactcactgc ccgcatcgcc atgggcctgc tggccgctac
     1681 gctgtgcgcc ggcagcgcct gggccgacaa gcccgaatgg gcggggcgcg gcaacggcaa
     1741 ctcgcaaggc gatggcccgc ccgggcagat gaaaaagcag cagcgctacg acgccgaccg
     1801 ccgtcactac gacgacggcc gctatcagcg ccccgaccgc caccgccgcg cgcccatcgc
     1861 cgtcaagccg ggcgcctact tcagcgatgg ccagcgcagc aacgcgcacc agtggtacag
     1921 cgcgcagtac cacgccggcc attgcccgcc gggtctggcg aaaaagaaca acggctgcct
     1981 gccgcccggc caggccaaga agtggcgcgt cggccagccg ctgccggtcg gcgtggtata
     2041 tcacgccgtg ccgcagcccg tgctgatcta ggtcggcccg ccgccacccg gttaccgcta
     2101 cgtgcgcgtg gccaacgacc tgcttttgat ggccgtcggc tcgcgtctgg tggtggatgc
     2161 gatcaccgat cttggacgag tcctgtgatc gccgcccgtc ggcccgcgac gatgaggggc
     2221 gccgcatgac cgagaatctg cccatgctga accgccgcca ttgccaccgc ctgctgggcg
     2281 ccgcagcgct catcaccctg tttcccggcc tgtcggcctg cgccgcgggc agcggcggca
     2341 tgccgggcta caccgtgtcg cagcagcggt tgaacgagtt gctggcgcgc cagtttcccg
     2401 tcacgcgcaa cctgctgggc gggctggccg acctgacctt gtcgtcgccg cgcctcggct
     2461 tgctgccggc ctccaaccgc atctcgacga tgttcgatct gggcctgacc gagcgcctgg
     2521 ccggctcgcg ctacagcggc ggcatggacc tggactacgg cctgcggctt gacctgcagg
     2581 aaggcgcggt gcgcatggcc gacgtgcgcg tcaaccgcct gaccatcgac cagttgccgc
     2641 gcacgcagca gcaactggtg tcgcagtacg cgcccgacat tgccggccgc ctgctgtccg
     2701 acatggtgct gtaccgcctg cccgccgaac agctcgcgct ggcgcgcaac atgggctggg
     2761 gcgcggcggc ggtgcgcgtg ctgcccgatg gcctgcgcat cgacatgggg caggccggca
     2821 cgcgctgatt ttcaagcaga acaggccgcc agcgcttgct gggcaagcgc aagcagctat
     2881 ctaaacggta gcttcgatca agccatcttg cagcccgcac ccgaatccga cagcgccttg
     2941 gcgtccacgc ccagcacctt gttctcgccc ttttcgacca cgcgcaggta gaaaccctgc
     3001 accgggttgt gggcgcggcc cgtagcctgc gctcggccat tggggcaaac gccgggcttt
     3061 cgtaggatgg cccaggcatt cacccattac caggagacac accatgcaca acacccacct
     3121 gcgcatcggc cacggcgcgc atctggtcat cgccttgcac ggctggtttg gccacgcggg
     3181 cggctggggg ccgttcacgc agcagctcaa cacgcaggac ttcagctacg tcttcatgga
     3241 ccagcgtggc tacggcggca tgcgccgatc cggcgggccc tacaccgtgg agcagatcgc
     3301 gcaggacgcg ctggcgctgg ccgaccgcct gggctggcag cgcttttcgc tcatcggcca
     3361 ctcgatgggc ggctcatcga tccagcaggt gctggccgcc gcgcccgagc gcgtgcgtgc
     3421 gctggtcgct ctcacgcccg tctcggccat cggcgtgccg tttgacgagc agggctggca
     3481 gctcttcagc gccgctggca gcgacgtaca ggcgcgccgc gccatcatcg acttcaccac
     3541 cggcaaccgc ctcacgggca cctggctcga cgccatggcc gccagcacgc aaaccaattc
     3601 cgacgacgag gccgtggccg gctacctgca gtcgtgggcc aagaccagct tcgtggacaa
     3661 gattcagggc caacagctgc cggtgttggt gctgccaggc gagcacgacc cggcgctggg
     3721 cgaagccgcc tgccgcgcca cctggatgca gcactacccc aacgcgcaac tcgaggtggt
     3781 gcgcaacgcc ggccactacc cgatggacga aacgccgatc atcctggcca ccacgatcga
     3841 gaagtttcta g
//
LOCUS       DAOMVO010000237         3843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6897_length_3843_cov_13.114044, whole genome shotgun sequence.
ACCESSION   DAOMVO010000237 DAOMVO010000000
VERSION     DAOMVO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6897_length_3843_cov_13.114044"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /gene="rlmJ"
                     /locus_tag="PKV30_10580"
     CDS             <1..456
                     /gene="rlmJ"
                     /locus_tag="PKV30_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRL30689.1"
                     /translation="TLFNWLGQDLSGWRAADAFAGTGVLGFEAASRGAAEVLLVEQDA
                     ALVAKLRAIKERLKADAVRVQRGDGVAALRALAPRSVDLVLLDPPFDAAELFAPALAA
                     AARAVQPQGWVYLEAPTAWDDAALVDAGLAPHRHLKAGAVHAHLLRPCA"
     gene            484..978
                     /gene="coaD"
                     /locus_tag="PKV30_10585"
     CDS             484..978
                     /gene="coaD"
                     /locus_tag="PKV30_10585"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRL30690.1"
                     /translation="MSRHVIAVYPGTFDPITLGHEDIVRRAVGLFDEVVIAVATAHHK
                     KTLFTLDERLELARDTASRYANVSVAPFDGLVRDFVVARGGKVMVRGVRGVTDFDYEF
                     QLAGMNRNLAPDVETIFMAPTATLQSVSSTLIREISQLGGDVAPYVAPMVLERLQQKR
                     AARG"
     gene            complement(1061..1834)
                     /locus_tag="PKV30_10590"
     CDS             complement(1061..1834)
                     /locus_tag="PKV30_10590"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRL30691.1"
                     /translation="MAAFDEEKVLTVHHWTDRLFSFTTTRSDSLRFSNGHFTMIGLRV
                     NDKPLLRAYSIVSPNYQETLEFLSIKVQDGPLTSRLQHIQPGDSVIVGHKPTGTLLID
                     YLLPGKRLYMFGTGTGLAPFMSIIRDPDTYEKFDEVVLVHGCRLVSELAYRDYITREL
                     REHEFLGEMVASQLKYYPTVTREPFPTQGRIPDLIENGQMARDLGVPPLNPQVDRVML
                     CGSPEMLKSIKHVLEARGFNEGNTTTPGDFVVERAFVEQ"
     gene            1975..3174
                     /locus_tag="PKV30_10595"
     CDS             1975..3174
                     /locus_tag="PKV30_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRL30692.1"
                     /translation="MNPSLPLSGLRVLDLSRVLAGPLAAQVLGDLGADVVKVEHPARG
                     DDTRDWGVRTGAHNSAYFNSMNRNKRSVTLDLQQPEGQRLARELALQSDVVIQNFKFG
                     GIAKMGLDYDTLAAERPGIVYCSITGYDPTGPEAARPGYDLVVQGETGLMGLNGEEGQ
                     APLKFGVAMVDLFTGMSAGQAILAALWARERNGGRGRHVQLALHDCGVMLSVYYGMEA
                     LIQQADPPKYGNAHPSIVPYGVFDAADGPLVITVGNNAQFVRFCQGVIGRPELADDPR
                     YATNAARAVHRHELLPIIKVELARRPRAELLESLVRAGIPCGEVLGVLDALTSPRTQA
                     AGGVQQATLGDGTPVPVQAPPYRFEGARLPIRHAPPTLGQHTAEVLGELGVDAARLDA
                     LRQGGVV"
     gene            3251..>3843
                     /locus_tag="PKV30_10600"
     CDS             3251..>3843
                     /locus_tag="PKV30_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL30693.1"
                     /translation="MHQQTLLAAFALTRRRALLATGALLAAGGAGGQSWPAKPIKLVV
                     PWPPGGPTDTGSRIFGLALADRLGQPVVVENRPGASGSIAYQQVIKTPGDGTTFVMLA
                     TPTMLAPHLYKSASYHPVNDLLPVGMVYDLPIVLVVNAASLPKVTDLAGLIAEAKSRG
                     GKLDYTSAGPGSFGHLSMEMLKHQGGFDAQHIAYKGSA"
BASE COUNT          585 a         1316 c         1320 g          622 t
ORIGIN      
        1 acgctgttca actggctggg tcaggacttg agcggctggc gggcggccga tgcgtttgcc
       61 ggcaccggcg tgctgggttt tgaagccgcc tcgcgcggcg cggccgaggt gctgctggtc
      121 gagcaggacg ccgcgctggt cgccaagctg cgcgcgatca aggagcggct gaaggccgac
      181 gccgtgcgcg tgcagcgcgg cgatggtgtg gcggcgctgc gggcgctggc gccgcgcagc
      241 gtcgatctgg tgctgctcga tccgccgttc gatgcggccg agctgttcgc ccccgcgctg
      301 gccgccgccg cgcgggcggt gcagccgcag ggctgggtgt acctcgaagc gcccacggcc
      361 tgggacgatg cagcgttggt cgacgcaggg ctggcgccgc accgccacct caaggccggc
      421 gccgtgcatg cgcatttgct gcggccctgc gcataatcgc cgccaccgca ggagagccac
      481 gccatgagcc gccacgtcat cgccgtctac cccggcacct tcgatcccat cacgctcggc
      541 cacgaagaca tcgtgcgccg cgccgtcggg ctgttcgacg aggtggtgat cgccgtcgcc
      601 acggcacacc acaagaagac gctgttcacg ctcgacgaac ggctggaact ggcgcgcgac
      661 accgccagcc gctacgccaa cgtcagcgtg gcgccgttcg acggcctggt gcgcgatttc
      721 gtcgtcgcgc gcggcggcaa ggtcatggtg cgcggcgtgc gcggcgtgac cgatttcgac
      781 tacgagtttc aactggccgg catgaaccgc aacctggcgc ccgacgtgga aaccatcttc
      841 atggcgccca ccgccacgct gcaatcggtc agcagcacgc tgatccgcga gatctcgcaa
      901 ctgggcggtg atgtggcgcc ctacgtggcg ccaatggtgc tggagcggct gcaacaaaag
      961 cgcgccgcgc gtggctgaac gctatcgaat caagagctgc ctgcgcttgc tggataagcg
     1021 cttacggcct atttgtatct gaatcgagag ccgggcagcc tcactgctcg acaaacgccc
     1081 gctccaccac gaagtcgccc ggcgtggtgg tgttgccttc gttgaagccg cgcgcttcca
     1141 gcacgtgctt gatcgacttc agcatctcgg ggctgccgca cagcatcacg cggtcgacct
     1201 gcgggttgag cggcggcacg cccaggtcgc gcgccatctg gccgttctcg atcaggtcgg
     1261 ggatgcgtcc ctgggtaggg aagggctcgc gcgtcaccgt cgggtagtac ttgagctggc
     1321 tggcgaccat ctcgcccagg aattcgtgct cgcgcagctc gcgtgtgatg tagtcgcggt
     1381 aggccagctc agacaccaac cggcagccgt gcaccagcac cacttcgtcg aatttctcgt
     1441 aggtgtcggg gtcgcggatg atgctcatga agggcgccag gcccgtgccg gtgccgaaca
     1501 tgtacaggcg cttgcccggc agcaggtagt cgatcagcag cgtgccggtc ggcttgtggc
     1561 cgacgatcac gctgtcgccc ggctgaatgt gctgcaggcg gctggtcagc gggccgtctt
     1621 gcaccttgat gctgaggaat tccagcgtct cctggtagtt cgggctgacg atggaatacg
     1681 cgcgcagcag cggcttgtcg ttcacccgca ggccgatcat ggtgaagtgg ccgttcgaga
     1741 agcgcaggct gtcgctgcgc gtggtggtga agctgaacag gcggtccgtc cagtggtgaa
     1801 cggtgaggac tttttcttcg tcgaaggcgg ccatgggcag ggtgctgtgc cgcagggtgc
     1861 ggcgggcgat gggggaaaac ccgcgattgt cgcaccgccc ttagaccgat ccgtgcatga
     1921 gcttatgctg cggcttccgg cgctggccgg gcgcatcgtt ttcaagaagc cgtcatgaac
     1981 ccctctcttc ccctctccgg cctgcgcgtg ctcgatctgt ctcgcgtgct ggccggcccg
     2041 ctggccgcgc aggtgctggg cgacttgggc gccgacgtcg tcaaggtcga gcacccggcg
     2101 cgcggcgacg acacgcgcga ctggggcgtg cgcaccggcg cgcacaacag cgcctacttc
     2161 aacagcatga accgcaacaa gcgctcggtc acgctcgatc tgcagcagcc cgaaggccag
     2221 cgcctggcgc gcgaattggc gttgcaaagc gatgtggtga tccagaactt caagttcggc
     2281 ggcatcgcca agatggggct cgattacgac acgctggccg ccgagcgccc gggcatcgtg
     2341 tactgctcca tcaccggcta cgaccccacc ggccccgagg ccgcgcgccc cggctacgac
     2401 ctggtggtgc agggcgaaac cggcttgatg ggcctgaacg gcgaggaggg ccaggcgccg
     2461 ctgaagttcg gtgtcgccat ggtcgacttg ttcaccggca tgtcggccgg gcaggcaatt
     2521 ctggcggcgc tctgggcgcg cgaacgcaac gggggccggg gccgccacgt gcaactggcg
     2581 ctgcacgact gcggggtcat gctgtcggtc tactacggca tggaggcact gatccagcag
     2641 gcggacccgc ccaagtacgg caacgcccac ccgtccatcg tgccgtatgg cgtgttcgac
     2701 gccgcagacg ggccgctggt catcaccgtg ggcaacaacg cgcagttcgt ccgcttttgc
     2761 cagggcgtga tcggccggcc cgagctggcc gacgacccgc gctacgccac caacgccgcg
     2821 cgcgcggtgc accggcacga gctgctgccc atcatcaagg tcgagctggc gcgccgcccg
     2881 cgcgccgagc tgctcgaatc gctggtgcgc gccggcattc cctgcggcga agtgctgggc
     2941 gtgctggatg ccctgacctc accgcgcacg caggccgccg gcggcgtgca gcaggccacg
     3001 ctgggcgacg gcacgccggt gccggtgcag gcgccgccct accgctttga aggcgcgcgc
     3061 ctgccgatcc gccacgcgcc gcccacgctg ggccagcaca cggccgaggt gctgggcgaa
     3121 ctgggcgtcg acgcggcacg ccttgacgcg ctgcgccagg gcggcgtggt ttgaagcctt
     3181 ttaggccgct ggcgcttgtc catcaagcgc gagcagctct tgtttttgta gtgaaaagga
     3241 ggcccgagcg atgcatcagc aaaccctatt ggccgctttt gcgctgaccc gccggcgcgc
     3301 gctgctcgcc accggcgccc tgctggccgc tggcggcgcc ggcgggcaaa gctggcccgc
     3361 caagccgatc aagctcgtcg tgccctggcc gcctggcggc ccgaccgaca ccggctcgcg
     3421 catcttcggc ctggcgctgg ccgaccgcct gggtcagccc gtggtggtcg aaaaccgccc
     3481 cggcgcttcc ggctccatcg cctaccaaca ggtcatcaag acgccaggcg acggcaccac
     3541 cttcgtcatg ctcgccacgc ccaccatgct ggcgccgcac ctgtacaaaa gcgccagcta
     3601 ccacccggtg aacgatttgc tgcccgtggg catggtgtac gacctgccca tcgtgttggt
     3661 cgtcaacgcc gcgtcgctgc ccaaggtgac cgacctggcg ggcctgatcg ccgaagccaa
     3721 gtcgcgcggc ggcaagctcg actacaccag cgccggcccc ggcagctttg gccacctgag
     3781 catggaaatg ctcaagcacc agggtggctt cgacgcccag cacatcgcct acaagggcag
     3841 cgc
//
LOCUS       DAOMVO010000238         3828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6936_length_3828_cov_13.280148, whole genome shotgun sequence.
ACCESSION   DAOMVO010000238 DAOMVO010000000
VERSION     DAOMVO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_3828_cov_13.280148"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3662
                     /locus_tag="PKV30_10605"
     CDS             <1..3662
                     /locus_tag="PKV30_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HRL30694.1"
                     /translation="WFFSQSEFLVRGGRVRWVDEQRQAPPVQLSDVQFVARNGYGRHQ
                     LRVDATPDAAWGDRFTLIGQFRQPFFSRHPGYWREWDGQAYADLGRVDVSRLRQYADL
                     KTDWGVDLQGGHGALRLWADLKKGVLTSATADVALGAVSATFDQRLQPLAFASLTGRI
                     GWRNPGSGMEVSTRDLHFVDADGLAWPGGNVRLGYRDGAGGLPAGGELVGDKLDLAAL
                     AKIAQRLPLPPAMHERLAQHPVQGLVERIDARWNGPLEAPQDWRVQTRVSGLAVAARA
                     APARPGGEPVEGIPGLEGAAIELEATPVSGHAALEIQNGALEFPGVFQEPRIPLAELK
                     ATARWRVREGGHIELDVDQLALNNQDATGSFKAHWQTAAGQQGAERFPGVLDLSGSFS
                     RANGARIHRYLPLGIPSSARDYVRNAVQKGEARDVAVRVKGDLREVTSPRAPPGSEFR
                     FAGKVHGVTLAYVPRALQPPEQAAWPALEDLAGELIFDHNSMQVKNASGRVQGHPGWQ
                     FTRVQAGIADLGKTRVLVDAEGRGLLASALGIVRASPVAGFIQHALDRATASGDAGLH
                     LKLDLPIERIRDAKVDGRITLAGNDVRITPDTPQLAQAQGAVTFSDTGFALQDTRVRL
                     LGGEARLSGGTQANGAAGAPSVLLRAVGTASADALREMADWAPLPALARQASGSAAYE
                     AQIGFRAGQPDVLVTSDLRGMAFDLPAPLAKPADAAWRLRYESGRVAGNDDRARFRVA
                     VADQLAVEYEREAESGRVLRGVIGIGPQAMPQLALPASGVVARLHLPRLDAEAWDAAL
                     TRVFGDDGNVPNTGDTSFVPTAWSLRAGELVLDEHTAHDVAASGTREGRTWRADLQAR
                     ELAGRIEYSEGASGRAGKLLARLSRLSIPAGGAEDQSLLAQPPAQIPALDVVVEEFEL
                     RGKKLGRLEVEAVNHDVTPARPGAGALQEWELSRLVLRAPEATFTANGKWDAQARGPA
                     LPLNPRAPRAAGDLRRTALDFKLEVRDAGKLLARFDMPGVLSRGKGQLDGRLSWRGAP
                     FSPHYPSMAGQLHVDVGAGQFLKADPGMAKLLGVLSLQSLPRRLTLDFRDLFSTGFAF
                     DFVRGDVEVQRGIASTNNLQMKGATAAVLMDGSADIDKETQNLRVLVVPEIDAGTAAL
                     VATAINPVIGIGAFVAQLVLKKPLIKAATREFHIGGSWDDPQVTRTNARAEGGVSAPA
                     SAPASAIEPQSEETS"
     gene            3659..>3828
                     /locus_tag="PKV30_10610"
     CDS             3659..>3828
                     /locus_tag="PKV30_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRL30695.1"
                     /translation="MKVAAIQTVSCTRVADNLRAAHALLAEAAQGGAELAVLPEYFCV
                     MGQRDTDKLALQ"
BASE COUNT          547 a         1369 c         1408 g          504 t
ORIGIN      
        1 actggttctt ctcgcaatcc gagttcctgg tgcgcggcgg ccgcgtgcgc tgggtcgacg
       61 agcaacgcca ggcgccgccg gtgcagctgt ccgatgtgca gttcgtggcg cgcaacggct
      121 atggccggca ccagctgcgt gtggacgcca cgccggatgc ggcgtggggc gaccgcttca
      181 cgctcatcgg ccagtttcgc cagccctttt tcagccgcca ccccggctac tggcgcgagt
      241 gggacggtca ggcctacgcc gacctgggcc gcgtcgatgt ctcccgcctg cgccagtacg
      301 ccgatctgaa aaccgattgg ggcgtcgatc tgcaaggcgg ccacggcgcg ctgcgcctgt
      361 gggccgacct gaagaagggc gtgctcacca gcgccaccgc cgacgtggcg ttgggcgccg
      421 tgtcggccac cttcgatcag cgcctgcaac cgctggcctt cgcctcgctc accgggcgca
      481 tcggctggcg caacccgggc agcggcatgg aggtcagcac gcgcgatctg cacttcgtcg
      541 atgccgacgg cctggcgtgg ccgggcggca acgtgcggct cggctaccgc gacggcgccg
      601 gcggcctgcc ggcgggcggc gagctggtgg gcgacaagct cgacctggcc gcgctggcca
      661 agatcgcgca gcgcctgccg ctgccgccag ccatgcacga gcggctggcg cagcacccgg
      721 tgcaggggct ggtcgagcgc atcgacgcgc gctggaacgg cccgctggag gcgccgcaag
      781 actggcgcgt gcagacgcgc gtcagcggcc tggcggtggc cgcgcgcgcg gcgccggcgc
      841 ggcccggtgg cgagccggtg gagggcattc ccggcctgga gggcgccgcc atcgagctgg
      901 aagccacgcc ggtcagtggc cacgccgcgc tggaaatcca gaacggcgcg ctcgagtttc
      961 ctggcgtgtt ccaggagccg cgcattccgc tggccgaact caaggccacg gcgcgctggc
     1021 gcgtgcgcga gggcgggcac atcgagctgg acgtggacca gctggcgctg aacaaccaag
     1081 atgccaccgg cagcttcaag gcgcactggc agaccgccgc cggccagcag ggcgccgagc
     1141 gctttcccgg cgtgctggat ttgtcgggct cgttcagccg cgccaacggc gcgcgcatcc
     1201 accgctacct gccgctgggc attcccagct cggcgcgcga ctacgtgcgc aacgcagtcc
     1261 aaaagggcga ggcgcgcgat gtggcggtgc gcgtcaaggg cgatctgcgc gaagtcacca
     1321 gcccgcgcgc gccgcccggc agcgaattcc gctttgccgg caaggtgcac ggcgtcacgc
     1381 tggcctacgt gccgcgcgcg ctgcagccgc ccgagcaggc cgcgtggccg gcactggagg
     1441 atctggcggg cgagctgatc tttgaccaca acagcatgca ggtgaagaac gccagcggcc
     1501 gcgtgcaagg ccacccgggc tggcagttca cgcgcgtgca ggccggcatc gccgatctgg
     1561 gcaagacgcg agtgctggtg gatgccgagg ggcgcggcct gctggcgtcg gcgctcggca
     1621 tcgtgcgtgc gtcgccggtg gccggcttca tccagcacgc gctggaccgc gccaccgcca
     1681 gcggcgacgc cgggctgcac ctgaagctcg atctgcccat cgagcgcatc cgcgacgcca
     1741 aggtcgacgg ccgcatcacg ctggccggca acgacgtgcg catcacgccg gacacgccgc
     1801 aactcgcgca ggcgcagggc gccgtcacct tcagcgacac cggttttgcg ctgcaggaca
     1861 cgcgcgtgcg cctgctgggc ggcgaggcgc gcctttcggg cggcacgcag gcgaatggcg
     1921 ccgcgggcgc gccgtcggtg ctgctgcgcg cggtgggcac cgccagcgcc gacgccctgc
     1981 gcgagatggc cgactgggca ccgctgcccg cgctggcgcg ccaggccagc ggcagcgcgg
     2041 cgtacgaggc gcagatcggc tttcgcgccg ggcagcccga cgtgttggtg accagcgacc
     2101 tgcgcggcat ggcgttcgat ttgccggcgc cgctggccaa gccggccgac gccgcctggc
     2161 gcttgcgata tgagagcggt cgggtggcgg gcaacgacga ccgcgcgcgc tttcgcgtcg
     2221 ccgtggccga tcaactggcg gtcgaatacg agcgcgaggc cgaatccggc cgcgtgctgc
     2281 gcggcgtcat cggcatcggc ccgcaggcca tgccgcaact ggcgctgccg gccagcggcg
     2341 tggtggcgcg tttgcacctg ccgcggctgg acgccgaggc gtgggacgcg gcgctgacac
     2401 gcgtgttcgg cgacgacggc aatgtcccca acacgggcga caccagcttc gtgcccaccg
     2461 cctggtcgct gcgcgccggc gaactggtgc tggacgagca caccgcgcac gatgtggccg
     2521 ccagcggcac gcgcgagggg cgcacctggc gcgccgacct gcaggcgcgc gaactggccg
     2581 gccgcatcga gtacagcgaa ggcgccagcg gtcgcgccgg caagctgctg gcgcggctgt
     2641 cgcggctgtc gattcccgct ggcggcgccg aagaccagag cctgctggcg caaccgccgg
     2701 cgcagattcc ggcgctcgac gtggtggtgg aagagttcga gctgcgcggc aagaagcttg
     2761 gccggctgga ggtcgaggcc gtcaaccacg acgtgacgcc agcccgccct ggcgccggtg
     2821 cgctacagga atgggagctg tcgcggcttg tcctgcgtgc gccagaggcc acttttactg
     2881 ccaacggcaa gtgggacgcg caggcgcgcg gccccgcgct gccgctgaac ccccgcgcgc
     2941 cacgcgccgc cggcgacctg cgccgcaccg cgctcgactt caagctcgag gtgcgcgacg
     3001 ccggcaagct gctggcgcgc ttcgacatgc cgggcgtgct gtcacgcggc aagggccagc
     3061 tggacggcag gctgagctgg cgcggcgcgc cgttctcgcc gcactacccc agcatggcgg
     3121 gccagctgca tgtggacgtg ggcgccggcc agttcctcaa ggccgacccg ggcatggcca
     3181 agctgctcgg cgtgctcagc ctgcagtcgc tgccgcgccg gctgacgctg gactttcgcg
     3241 atttgttctc gaccggcttc gcgttcgact tcgtgcgcgg cgacgtcgag gtgcagcgcg
     3301 gcatcgccag caccaacaac ctgcagatga aaggcgccac ggccgccgtg ctgatggacg
     3361 gcagcgccga catcgacaag gaaacgcaga acctgcgtgt gctggtcgtg cccgagatcg
     3421 acgccggcac ggccgcgctg gtggccaccg ccatcaaccc ggtgatcggc atcggcgcct
     3481 tcgtggcgca gttggtgctc aagaagccgc tgatcaaggc cgccacgcgc gaattccaca
     3541 tcggcggcag ctgggacgac ccgcaggtca cgcgcaccaa tgcacgcgcc gaaggcggcg
     3601 tgagcgcacc ggcctcggcg cccgcatccg ccatcgagcc gcagagcgag gaaacctcat
     3661 gaaagtcgcc gccatccaga cggtctcgtg tacccgcgtg gccgacaacc tgcgcgccgc
     3721 gcacgcgctg ctcgccgaag ccgcgcaagg cggtgccgag ctggccgtgc tgcccgaata
     3781 cttctgcgtc atgggccagc gcgacaccga caagctggcg ttgcagga
//
LOCUS       DAOMVO010000239         3824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6948_length_3824_cov_14.789069, whole genome shotgun sequence.
ACCESSION   DAOMVO010000239 DAOMVO010000000
VERSION     DAOMVO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_3824_cov_14.789069"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..2192
                     /locus_tag="PKV30_10615"
     CDS             66..2192
                     /locus_tag="PKV30_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HRL30696.1"
                     /translation="MAGTLPAVTVRDSAVGDGSTNSKTQLRATRTEIGKGNQELRDIP
                     QSVTVMTEKLMQDRNLDDFREVLRTTAGVTFQAGETGEEDVRLRGFSLGVAGDIYRDG
                     MRDGTLYERDTFNDDRVEVIKGSASMLFGKGSTGGVVNQVSKQPFLMNQHEASMTLGS
                     GNEKRVAGDFNWQTGQDSAFRLNVMAQDAKNWGAKQRKLGIAPTFRWGIGTRDEFSVG
                     LYHLDTDGRSAFSHPWFIRDGRIVPTLPARNFYGFNSDKLDTQATYGTISHTHRFDDG
                     GQLKTQFRHGRYERDLWASVIRFGTTGGQPTTIDNWGPNTIVTRAPKGRIGASDTTQL
                     QSDYSNTYHWGGREHKLIAGIDMYYDKAKRNNNFAGPASTLTTTVDHPNNGDWRPDTR
                     GEPTYNHFNARNLGLYVQDMVSMTDTLKLVAGLRFDHFKASYDTATTTAANGTITPGY
                     SFGKSESLWSPRVGLLFQPSETQSYYVSFGTSYNTSGDAYQFTPNSPNQVLANTPPEK
                     SRNFEIGGKWDVLEKRMSLGAAVFYSEKYNERNQDPDSAATQYLLSGKRYAAGMEFNA
                     AGRITPAWEVFWNHTWIPLAKISRSNQVLAANGGGAQVEGDRPGLTPRHSGSVWTTYR
                     VFPKLRLGLGANYRSSQNPDGSRAVKAAGFVTWDMMAEYSFTETTTLKLNVSNLTDKL
                     YADTLYRGFYGPGQPRRIQLTLKHLF"
     gene            2260..2949
                     /locus_tag="PKV30_10620"
     CDS             2260..2949
                     /locus_tag="PKV30_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HRL30697.1"
                     /translation="MLLHLKNLLTESELAQGRALLLADDAPWIDGRRSAGEQAVQQKN
                     NQQLAQDSASAAALRAVVLGALRRDPLLFSAALPKKIFNPLFNRYSGESNHYGAHVDG
                     AVLHSRAPDQWVRTDLSCTVFFSRPDEYDGGELRIHDTYGDQRVKLPAGDAVLYPGTS
                     VHEVRPVTRGARIASFFWIESMVRSGEQRALLFEMDMALLALRQQHGESAAATRLTGV
                     YHNLLRQWSST"
     gene            2993..>3824
                     /locus_tag="PKV30_10625"
     CDS             2993..>3824
                     /locus_tag="PKV30_10625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRL30698.1"
                     /translation="MSIVRKQLSILKSPTPTPATLADYAEAARHALDDAAWAFFEGGA
                     ADEITLRENIAAWQRLSLWPLVLRPLAGGHTRVTLAGRELAHPILLAPVAHQKLAHPD
                     GEWASALAAAAQGAGYVLSTQSDVPMESIAQAVLAEAGCGPLWFQLYWQADRGFNHAL
                     VQRAEAAGFEAIVLTADAPVQGVREREWRHPLPPGASAPAVHLQHAPAAPPAPDTYCA
                     GVPAHAPTWDDVAWLQAQTRLPVWLKGVLHPQDARQVQSMGVAGLIVSNHGGRTLDTA
                     V"
BASE COUNT          700 a         1362 c         1232 g          530 t
ORIGIN      
        1 cgcagtccgc agcgacggcg gccgacgcgc ccgcggcgca gcaagcgccc gccagcgcgg
       61 cgccggtggc cggcacgctg ccggccgtca cggtgcgcga ttcggccgtc ggcgacggct
      121 cgaccaacag caagacgcaa ctgcgcgcca cccgcaccga gatcggcaag ggcaaccagg
      181 agctgcgcga cattccgcaa agcgtgacgg tgatgaccga aaagctgatg caggaccgca
      241 acctggacga cttccgcgaa gtgctgcgca ccaccgccgg cgtcaccttc caggccggcg
      301 aaacgggcga ggaagacgtg cgcctgcgcg gcttttcgct cggcgtggcg ggcgacatct
      361 accgcgacgg catgcgcgac ggcacgctgt acgagcgcga caccttcaac gacgaccgcg
      421 tcgaagtgat caagggctcg gcctcgatgc tgttcggcaa gggctcgacc ggcggcgtgg
      481 tcaaccaggt cagcaagcag cccttcttga tgaaccagca cgaggccagc atgacgctgg
      541 gcagtggcaa cgagaagcgc gttgcgggcg acttcaactg gcagaccggc caggattcgg
      601 cattccgcct caacgtgatg gcccaggacg ccaagaactg gggcgccaag cagcgcaagc
      661 tgggcattgc gcccacgttc cgctggggca tcggcacgcg cgacgagttc tcggtcgggc
      721 tgtaccacct cgacaccgat ggccgctcgg ccttcagcca cccctggttc atccgcgatg
      781 gccgcatcgt gcccacgctg ccggcgcgca atttctacgg cttcaacagc gacaagctcg
      841 acacgcaggc cacctacggc accatttcgc acacccaccg ctttgacgat ggcggccagc
      901 tgaaaacgca gttccgccac ggccgctacg agcgcgattt gtgggccagc gtgatccgct
      961 tcggcaccac gggcggccag cccaccacca tcgacaactg gggcccgaac accatcgtta
     1021 cgcgcgcgcc caagggccgc atcggcgcca gcgacacgac gcagctgcaa agcgactaca
     1081 gcaacaccta ccactggggt gggcgcgagc acaagctgat cgccggcatc gacatgtatt
     1141 acgacaaggc caagcgcaac aacaactttg ccggccccgc cagcacgctg accaccaccg
     1201 tcgaccaccc caacaacggc gactggcggc ctgacacgcg cggcgagccg acgtacaacc
     1261 acttcaatgc gcgcaacctg ggcctgtacg tgcaggacat ggtctcgatg accgacacgc
     1321 tgaagctggt ggccggcctg cgcttcgacc atttcaaagc cagctacgac accgccacca
     1381 ccaccgccgc caacggcacc atcacgccgg gctacagctt tggcaagagc gaatcgctgt
     1441 ggagcccgcg cgtcggcctg ctgtttcagc caagcgagac gcagtcgtat tacgtgtctt
     1501 tcggcacctc gtacaacacc tcgggcgacg cctaccagtt cacgcccaac agccccaacc
     1561 aggtgctggc caacacgccg cccgagaaaa gccgcaactt cgagatcggc ggcaagtggg
     1621 acgtgcttga aaaacgcatg tcgctgggcg cggccgtgtt ctattcagaa aagtacaacg
     1681 aacgcaacca ggacccggac agcgccgcca cgcaatacct gctgtcgggc aagcgctacg
     1741 ccgcgggcat ggaattcaac gccgccggcc gaatcacccc ggcatgggaa gtgttctgga
     1801 accacacctg gattccgctg gccaagatca gccgcagcaa ccaggtgctg gcggccaacg
     1861 gcggcggcgc gcaggttgaa ggcgaccgcc ccggcctgac accgcggcac agcggcagcg
     1921 tgtggaccac ctaccgcgtt tttccgaagc tgcgcctggg cctgggcgcc aactaccgca
     1981 gcagccagaa ccccgacggc agccgcgcgg tgaaggcggc cggcttcgtc acctgggaca
     2041 tgatggcgga atacagcttc accgaaacca ccacgctcaa gctcaacgtc agcaacctga
     2101 ccgacaagct gtacgccgac acgctgtacc gcggcttcta cgggccgggg cagccgcgcc
     2161 gtatccagct gacgctgaaa cacctgttct gatcgcacaa gttcttatcg ttcgctcggc
     2221 acgcgccgcc ttcgggcggc gatgtgcttt tttgacacca tgctgctgca cctgaaaaac
     2281 ctgctcaccg aatccgaact ggcgcaaggc cgcgcgctgc tgctggccga tgacgcgccg
     2341 tggatcgacg gccgccgcag cgctggcgag caggcggtgc agcaaaagaa caatcagcag
     2401 ctggcgcaag acagcgccag cgccgccgcg ctgcgcgcgg tggtgctggg cgcgctgcgg
     2461 cgcgacccgc tgctgttctc ggccgcgctg cccaagaaga tcttcaaccc gctgttcaac
     2521 cgctacagcg gcgagtccaa ccactacggc gcgcacgtcg atggcgccgt gctgcattcg
     2581 cgcgcgcccg accagtgggt gcgcaccgac ctgtcgtgca cggtgttttt cagccgccct
     2641 gacgagtacg acggcggcga gctgcgcatt cacgacacct atggcgacca gcgcgtgaag
     2701 ctgcccgcgg gcgacgccgt gctctacccc ggcaccagcg tgcacgaggt gcggcccgtc
     2761 acgcgcggcg cgcgcatcgc cagctttttc tggatcgaga gcatggtgcg ctcgggcgag
     2821 cagcgcgcgc tgctgttcga gatggacatg gcgctgctgg cgctgcgcca gcagcatggc
     2881 gaaagcgccg cggccacgcg cctgacgggc gtgtatcaca acctgctgcg ccagtggtcg
     2941 agtacatgag tcttcgccag ccggccgtca tggtaaaaat gcccgccagc gcttgtccat
     3001 cgtgcgcaag cagctatcaa ttttgaaatc gcccacccca actccagcaa cgcttgccga
     3061 ctacgccgag gccgcccggc acgcgctgga cgatgcggcg tgggcctttt ttgagggcgg
     3121 cgcggcggac gagatcacac tgcgcgaaaa catcgccgcc tggcagcgct tgagcctgtg
     3181 gccgctcgtt ctgcgtccgc tggctggcgg ccacacgcgc gtgacgctgg ccggccgcga
     3241 actggcgcac ccgatcctgc tggcgccggt ggcgcatcag aagctggcgc accccgatgg
     3301 ggaatgggcc agcgcgctgg ccgccgcggc gcagggcgcg ggctacgtgc tcagcaccca
     3361 atccgatgtg ccgatggaaa gcatcgccca ggccgtgctg gccgaggcgg ggtgcgggcc
     3421 gctgtggttt cagctttatt ggcaggccga tcgcggcttc aaccacgcgc tggtgcagcg
     3481 ggccgaggcg gccggtttcg aggccatcgt gctgaccgcc gacgcgcccg tccaaggcgt
     3541 gcgcgagcgc gaatggcgcc acccgctgcc gcccggcgcc agcgcgccgg ccgtgcatct
     3601 gcagcacgcg cccgccgcgc caccagcgcc cgacacctac tgcgccggtg tgcccgcaca
     3661 cgcgccgacc tgggacgacg tggcctggct gcaggcgcag acgcggctgc cggtgtggct
     3721 caagggcgtg ctgcacccac aggacgcgcg ccaggtgcag tcgatgggcg tcgccggcct
     3781 catcgtcagc aaccacggcg ggcgcacgct ggacacggcc gtgg
//
LOCUS       DAOMVO010000240         3816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_6969_length_3816_cov_13.909599, whole genome shotgun sequence.
ACCESSION   DAOMVO010000240 DAOMVO010000000
VERSION     DAOMVO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_3816_cov_13.909599"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2403
                     /locus_tag="PKV30_10630"
     CDS             <1..2403
                     /locus_tag="PKV30_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRL30699.1"
                     /translation="FLAAIVLAFWYLRVEEVRREQEAVKRDVEYSQQRLRLRLLERQE
                     QLMRLARDMADREMSTLQFDIRAESLVSQYPELMALSWIDGQKRLRATQSSPSVSPLR
                     EWAADGKVKPGEVEDGYSLARDLRQPVYAQPLAAEGAEPVLYLHVPITPRAQFGGELL
                     AEYSVEGLLRYGVPSDVLAKYAVALMDAKGKLLAGQTIPDRPGFSERLPWTGKVNEFD
                     IPVSLVGNGLVLRAQAWRTSQGVVGGTLFWLVAVLAAMTTWMLIANWRQSQRRQQAQQ
                     ALLAETTFRRAMENSMLTGMRALDLQGHIIYVNPAFCQMTGWSEDELIGQSEPFSYWP
                     LEDRDELAARLVDELGGLADPAGFQMRVKRKDGSLFDARLYVSPLIDAVGKQTGWMAS
                     MTDITEPNRVRRQLSASYERFTTVLEALDASVSVAPLGSAELLFANKLYRQWFGGTTE
                     GHLKLVMRAGTPETQLSEESLDAVDAFAGLPLDSIASAPLAERAEVYVEPLGKWLEVR
                     SRYLNWVDGRLAQMVIATDITARHQAQELAAAQAERAQTASRLITMGEMASSVAHELN
                     QPLTAISNYCTGMISRIKGGSIGQDDLLGALDKTARQAQRAGQVVQHIRSFVKRSEPR
                     RQWSEVPTMVEQVVELAEIDLRRRQVRLTHHVAPQLPALHVDPILIEQVLINLIKNGA
                     ESVDAAKRATSNRLVEVRVTQRDIEHQSTVQFSVTDSGRGLSPEALERLYEAFFSTKN
                     DGLGIGLNLCRSIVESHQGRIQAENLYNAGQVVGCRFSFWIPAGPVQGASPSDNPSKA
                     MA"
     gene            2400..3026
                     /locus_tag="PKV30_10635"
     CDS             2400..3026
                     /locus_tag="PKV30_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL30700.1"
                     /translation="MSLIPKRGTVYVVDDDEAVRDSVQWLLEGQDFRVRSFESSEVFL
                     ARYDPREVACLIVDIRMDGMSGLELQDRLIERNSPLPIAFITGHGDVPLAVDTMKKGA
                     LDFIQKPFNEQQLVPLVERMLEQARANFAEHQQAASRDALLSKLTGREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLGANTVADLLKIALGQNAGKA"
     gene            3116..>3816
                     /locus_tag="PKV30_10640"
     CDS             3116..>3816
                     /locus_tag="PKV30_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRL30701.1"
                     /translation="MTAQLIDGNALARQVRTDVAGRVAALKARGVQPQLNVILVGEDP
                     ASQVYTRNKVADSEQTGLAATLERYPADMTEADLLARIAALNADASVHGILVQLPLPA
                     HMDSHAVIEAIAPQKDVDGFHVASAGALMVGQPGFRPCTPYGCMKMLESIGMQDLRGK
                     HAVVIGRSNIVGKPMAMMLLAANATVTICHSGTPDVKAQTLQGDIVVAAVGKRNVLTA
                     DMVKPGAVVIDVGMNR"
BASE COUNT          679 a         1291 c         1258 g          588 t
ORIGIN      
        1 tttctggcgg ccatcgtgct cgccttctgg tacctgcgcg tcgaggaagt gcggcgcgag
       61 caggaggccg tcaaacgcga cgtcgaatac agccagcagc gcctgcgcct gcgtctgctg
      121 gagcggcaag agcagctgat gcgcctggcg cgcgacatgg ccgaccgcga gatgagcacg
      181 ctgcagttcg acatccgtgc cgaatcgctg gtgtcgcagt acccggagct gatggcgctc
      241 agctggatcg acggccagaa acgcctgcgc gccacgcagt cgtcgcccag cgtatcgccg
      301 ctgcgcgagt gggcggccga tggcaaggtc aagcccggcg aggtggaaga cggctacagc
      361 ctggcgcgcg atctgcgcca gcccgtctac gcgcagccgc tggccgccga aggggccgag
      421 ccggtgcttt acctgcacgt gccgatcacc ccgcgcgcgc agttcggcgg cgagctgctg
      481 gccgagtact cggtcgaagg gctgctgcgc tacggcgtgc cgtccgacgt gctggccaag
      541 tacgccgtcg cgctgatgga cgccaagggc aagctgctgg ccggccagac cattcccgat
      601 cgccctggtt tttcagagcg gctgccgtgg accggcaagg tgaacgaatt cgacattccc
      661 gtgtcattgg tcggcaatgg cctggtgctg cgggcgcagg cctggcgcac ctcgcagggc
      721 gtggtgggcg gcacgctgtt ctggctggtg gcggtgctgg ccgccatgac cacctggatg
      781 ctgatcgcca actggcgcca gtcgcagcgc cgacagcagg cacagcaggc actgctggcc
      841 gaaacgacct ttcgccgcgc aatggaaaac tccatgctga ccggcatgcg cgcgctcgac
      901 ctgcaaggcc acatcatcta cgtcaacccc gcgttctgcc agatgaccgg ctggagcgag
      961 gacgagttga ttggccagtc cgagcccttc tcgtactggc cgctggaaga ccgcgacgag
     1021 ctggcggcgc ggctggtgga cgagctgggc ggcctggccg acccggccgg ttttcagatg
     1081 cgtgtcaagc gcaaggacgg ctcgctgttc gacgcgcgcc tgtacgtgtc gccgctgatc
     1141 gatgccgtgg gcaagcagac cggctggatg gcgtcgatga ccgacatcac cgaaccgaac
     1201 cgcgtgcggc gccagctttc agcatcgtat gagcgcttca cgaccgtcct ggaagcgctg
     1261 gatgcttctg tttcagtagc gccgctgggc agcgcggaac tgctgttcgc caacaagctg
     1321 taccgccagt ggtttggcgg caccaccgaa gggcacctca agctggtgat gcgcgccggc
     1381 acgcccgaga cgcagctgtc cgaggaaagc ctggacgccg ttgacgcctt tgccggactg
     1441 ccgctggaca gcatcgcgtc cgccccgctc gccgagcgcg ccgaggtgta cgtcgagccg
     1501 ctgggcaagt ggctcgaagt gcgctcgcgc tacctgaact gggtcgatgg ccgcctggcc
     1561 caaatggtga ttgccaccga catcaccgcg cgccaccagg cgcaggagct ggccgccgcg
     1621 caggccgaac gcgcgcaaac cgccagccgt ttgatcacca tgggcgagat ggcctccagc
     1681 gtggcgcacg agctgaacca gccgctgacg gccatcagca actactgcac cggcatgatt
     1741 tcgcgcatca agggcggcag catcgggcag gacgatctgc tgggcgcgct cgacaagacc
     1801 gcgcgccagg cgcagcgcgc cggccaggtg gtgcagcaca tccgcagctt cgtcaagcgc
     1861 agcgagccgc gccgccagtg gtccgaggtg ccgaccatgg tcgagcaggt ggtcgagctg
     1921 gccgagatcg acctgcgccg ccgccaggtg cggctgacgc accacgtggc gccgcagttg
     1981 ccggcgctgc acgtcgaccc gatcctgatc gagcaggtac tgatcaacct gatcaagaac
     2041 ggcgccgaat cggtggacgc ggccaagcgc gccaccagca accggctggt cgaagtgcgc
     2101 gtgacgcagc gcgacatcga acaccagtcc accgtgcagt tcagcgtcac cgacagcggg
     2161 cgcggcctgt cgcccgaagc gctcgagcgg ctgtacgaag ccttcttctc gaccaagaac
     2221 gacggcctgg gcatcggcct caatctgtgc cgctccatcg tcgagtcgca ccagggccgg
     2281 atacaggccg agaacctcta caatgccggc caagtcgtgg gctgccgctt cagtttctgg
     2341 attccggccg gcccggttca gggcgcgagt ccgtccgaca acccctccaa ggcaatggca
     2401 tgagtctgat tccgaagcgc ggcacggtgt acgtggtcga cgacgacgag gcggtgcgcg
     2461 attcggtgca atggctgctc gaaggccagg atttccgcgt gcgcagcttc gaatcgtccg
     2521 aggttttcct ggcccgctac gacccgcgcg aagtggcctg cctgatcgtc gacatccgca
     2581 tggacggcat gagcgggctg gagctgcaag accgcctgat cgagcgcaat tcgcccctgc
     2641 ccatcgcctt catcaccggc cacggcgacg tgccgctggc cgtcgacacc atgaaaaaag
     2701 gcgccctgga tttcatccag aagcccttca acgaacagca actggtgccg ctggtcgagc
     2761 gcatgctgga gcaggcgcgc gccaactttg ccgagcacca gcaagccgcc agccgggacg
     2821 ccttgctgtc caagctgacg ggccgcgagg cgcaggtgct cgagcgcatc gtcgccggcc
     2881 gcttgaacaa gcagatcgcc gacgatctgg gcatcagcat caagacggtg gaagcgcacc
     2941 gcgccaacat catggaaaag ctgggcgcca acaccgtggc cgatctgctg aagatcgccc
     3001 tcggccagaa cgcgggcaag gcctgagccg cacgcccacc gccgacactc ttattttgat
     3061 agctgctcgc gcttgactga caagcgccag cggctttttt cattcataaa ccaccatgac
     3121 agctcaattg atcgacggca acgccttggc ccggcaggtg cgcactgacg tggccggccg
     3181 cgtcgccgcg ctcaaagccc gtggcgtgca gccgcagctc aacgtcatcc tggtcggcga
     3241 agacccggcc agccaggtct acacgcgcaa caaggtcgcc gacagcgagc agacgggcct
     3301 tgccgccacg ctggagcgct acccggccga catgaccgaa gccgatctgc tggcgcgcat
     3361 tgccgcgctg aacgccgacg ccagcgtgca cggcatcctg gtgcaactgc cgcttcccgc
     3421 gcacatggac agccacgccg tcatcgaagc catcgcgccg cagaaagacg tggacggctt
     3481 tcacgtcgcc tcggccggcg cgttgatggt cggccagccc ggctttcggc cctgcacgcc
     3541 ctacggctgc atgaagatgc ttgaatccat cggcatgcag gatttgcgcg gcaagcacgc
     3601 cgtggtgatt ggtcgcagca acatcgtcgg caaacccatg gccatgatgc tgctggccgc
     3661 caacgccacg gtcaccatct gccacagcgg cacgcccgat gtgaaggcgc agacgctgca
     3721 gggcgacatc gtggtcgccg ccgtcggcaa gcgcaacgtg ttgacggccg acatggtcaa
     3781 gcctggcgcg gtcgtcatcg acgttggcat gaaccg
//
LOCUS       DAOMVO010000241         3798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7005_length_3798_cov_16.188619, whole genome shotgun sequence.
ACCESSION   DAOMVO010000241 DAOMVO010000000
VERSION     DAOMVO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7005_length_3798_cov_16.188619"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKV30_10645"
     CDS             <1..537
                     /locus_tag="PKV30_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRL30702.1"
                     /translation="LQNACAHHLDAVVEKLRDAGAELEAIGGPDCQGGPKAHAIHIRS
                     LGGRALKGQGFRTTEYPGFPTDMQAQFMALNCVASGASVVTETIFENRFMHVNELVRL
                     GANIHVDGRTAMIDGVQQLSGATVMATDLRASASLVIAGLVADGETLVDRIYHLDRGY
                     DQMEEKLRGIGADIERVT"
     gene            666..1316
                     /gene="hisG"
                     /locus_tag="PKV30_10650"
     CDS             666..1316
                     /gene="hisG"
                     /locus_tag="PKV30_10650"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRL30703.1"
                     /translation="MITLALSKGRIFDETLPLLAAAGIAVLEDPEKSRKLILPTNQAG
                     VRVVLVRATDVPTYVEYGGADLGVTGLDTLIEHGGAGLYQPLDLQIAKCHMAVAAPVG
                     FDYQRAVRQGSRITVATKYTTIARDFFAEKGVHVDLIKLYGSMELAPLTGMADAIVDL
                     VSTGKTLKANNLVEVEHIMDISSRLVVNQAALKLKTAPIRRIIDAFSTAVNAPQTI"
     gene            complement(1368..1886)
                     /locus_tag="PKV30_10655"
     CDS             complement(1368..1886)
                     /locus_tag="PKV30_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30704.1"
                     /translation="MADGELFWIERPVAALPFRHESQGGAPKALQALIDCGAKTLALT
                     WRLDWQALGADERRWIEGGEYLPALGFLWLERAAALIRWSVQHQQPVAILELPSRRLT
                     QLSISDLMHVMGSDVWLADAQVALVWGFAHEALTHPQATRWPLPCRGAVYPSGDGLDV
                     GWWAAASDIAQI"
     gene            complement(1912..2775)
                     /locus_tag="PKV30_10660"
     CDS             complement(1912..2775)
                     /locus_tag="PKV30_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30705.1"
                     /translation="MRIESVHASGSRPTSPPDHSLMSQPAPAHVDTLRWKLADASERR
                     QLMATHGGRPQPHAEPAPQASSAPSSLLHAPLAAGVFQDLRYPVPNTFRGADGSPNKF
                     DLTFRDRSGFPNPTSHAYLNRTTPAPGNSWKGLRLDHGPNPTIGGASNWHWNHKGAGG
                     FGIPDHTAATRFESGVGRTLQAAKPLGRAAVVVGAALDGFSLAKEVQTSRKTGDWSNT
                     AQESARIAGGWTGAVVGAKAAGSAGAAIGTLIAPGIGTVVGGAVGGLIGGAVGYIAGS
                     GVGSTIAGWFK"
     gene            3032..>3798
                     /locus_tag="PKV30_10665"
     CDS             3032..>3798
                     /locus_tag="PKV30_10665"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRL30706.1"
                     /translation="MPTTAAPLRLSTAQPDFETRFSARLHWSADQDEAIEQRVKAILA
                     DVRARGDAAVLEYTARFDGLQAASMAELELKQSDFKAAFDSLPAEQQQALQEAAARVR
                     RYHEWQKKQGGETATYRDDDGTLLGQKVTPLDRVGIYVPGGKAAYPSSLIMNAVPAHV
                     AGVGEIIMVVPTPVRGSIATGGSDSKQPPALDGQAPAAIDSGVAGERNPLVLAAAHVA
                     GVTRAFTIGGAQAIGALAYGTATIPKVDKITGPGNAYV"
BASE COUNT          636 a         1305 c         1277 g          580 t
ORIGIN      
        1 ctgcaaaacg cctgcgcgca ccacctcgac gcggtggttg aaaaactgcg cgacgccggc
       61 gccgagctgg aagccatcgg cggccccgac tgccagggcg gccccaaggc gcacgccatc
      121 cacatccgca gcttaggggg aagggcgctg aagggccagg gctttcgcac caccgaatac
      181 cccggcttcc cgaccgacat gcaggcgcag ttcatggcgc tcaactgcgt ggccagcggc
      241 gccagcgtgg tcaccgagac catctttgaa aaccgcttca tgcacgtcaa cgagctggtg
      301 cgcctgggcg ccaacatcca cgtcgatggc cgcaccgcga tgatcgacgg tgtgcagcaa
      361 ctcagcggcg ccaccgtgat ggcgaccgac ctgcgcgcct cggcctcgct ggtcatcgcc
      421 ggtctggtgg ccgacggcga gacgctggtc gatcgcatct accacctgga tcggggttac
      481 gaccagatgg aagaaaagct gcgcggcatt ggggcggaca ttgagcgggt gacgtaaagc
      541 atgaaaacca gaggcgaaga acgcgaaggt ttcgcgaagg acgcgaaaga atcgaaaggt
      601 ttttctttcg cgtccttcgc gggcttttgc gtccttcgcg tccggtcaag tttgggagct
      661 acaaagtgat caccctcgcc ctctccaaag gccgcatctt cgacgagacc ctgccgctgc
      721 tggccgctgc cggcatcgcc gtgctggaag acccggaaaa gtcgcgcaag ctcatcttgc
      781 cgaccaacca ggcgggcgtg cgcgtggtgc tggtgcgcgc caccgacgtg ccgacctacg
      841 ttgaatacgg cggcgccgac ctgggcgtga cggggctcga tacgctgatc gaacacggcg
      901 gcgccggcct gtaccagccg ctcgatctgc aaatcgccaa gtgccacatg gccgtggctg
      961 cgcctgtggg ctttgattac cagcgcgcgg tgcgccaggg cagccgcatc accgtggcca
     1021 ccaaatacac caccatcgcg cgcgacttct ttgccgaaaa aggcgtgcac gtcgatctga
     1081 tcaagctcta cggcagcatg gaactggcgc cgctgaccgg catggccgat gccatcgtcg
     1141 atctggtcag caccggcaag acgctgaaag ccaacaacct ggtcgaggtc gagcacatca
     1201 tggacatcag ctcgcgcctg gtggtcaatc aggcggcgct caagctcaag acggcgccga
     1261 tccggcgcat catcgatgcg ttttcgacgg ccgtgaacgc ccctcagacg atataaagtt
     1321 gatagctgtc ggcgcttgat ggacgggcgc cagagccctt tttcacttca gatttgtgcg
     1381 atgtcgctgg cggccgccca ccagccgaca tcgagtccgt cgccgctcgg atagaccgcg
     1441 ccgcggcacg gcaagggcca gcgggtggcc tgcgggtgcg tgagcgcctc gtgcgcgaag
     1501 ccccacacca gcgccacctg cgcgtccgcc agccacacgt cgctgcccat cacatgcatc
     1561 aggtcgctga tcgacaactg agtcaggcgg cgcgagggca gctcgaggat cgccacgggc
     1621 tgctggtgct gcacgctcca gcggatcaac gcggcggccc gctccagcca gagaaaaccc
     1681 aatgcgggca ggtactcgcc gccctcgatc cagcgccgct cgtcggcgcc cagcgcctgc
     1741 cagtccagcc gccaggtcag ggccagcgtc ttggcgccgc agtcgatgag ggcctgcagc
     1801 gctttcggcg cgccgccctg cgattcgtgt cggaacggca atgccgcaac ggggcgctcg
     1861 atccagaaga gttcaccgtc tgccatggcg cccgagtgtg ccacgcggcc tttacttgaa
     1921 ccaaccggcg atggtggagc cgacgccgga gcctgcgatg tagcccaccg cgccgccgat
     1981 caggccaccg acggccccgc cgaccaccgt gccgatgccg ggggcgatca gggtgccaat
     2041 ggctgcacct gcggatcccg cggccttggc gccgacgacc gcgccggtcc agccgccggc
     2101 gatgcgtgcg ctctcctgcg ccgtgttgct ccagtcgccg gtctttcggc tggtctgaac
     2161 ctccttggcc agagaaaaac cgtccagcgc cgcgcccacg acgaccgcgg cgcgccccag
     2221 cggcttggcg gcctgcagcg tgcggccgac gccgctctcg aagcgcgtgg ccgcagtgtg
     2281 gtcggggatg ccgaagccgc ccgcgccctt gtggttccag tgccagttgc tggcgccgcc
     2341 gatggtcggg ttcgggccat ggtcgagccg caggcctttc cagctgttgc cgggcgcggg
     2401 cgtcgtgcgg ttcaggtagg cgtggctggt cgggttcgga aagccagagc gatcgcggaa
     2461 agtcaggtcg aacttgttcg ggctgccatc ggcgccgcga aaggtgttcg gaacgggata
     2521 gcgcagatcc tggaacacgc ccgcggccag cggcgcgtgc agcaggctgc tgggcgccga
     2581 actggcctgc ggcgcgggct cggcatgggg ctgcggacga cctccgtgtg tcgccatcaa
     2641 ctgccggcgc tcgctggcgt ccgccagctt ccagcgcagc gtgtcgacat gcgccggtgc
     2701 gggttgagac atgaggctgt ggtcgggcgg cgacgtgggg cggctgccgc tggcgtgcac
     2761 ggattcgatg cgcatggtgg gtctggtcgg gaggctcagt cgaggaatat gtccatcgtg
     2821 tgtcagatcg acccgcttga atacccggac gcttccgagc tgggatttct cccagctggt
     2881 ggggttacca ggccgaactc gctgtgcgcc gcgcgcctga tcacgcgcag ggcgccgcca
     2941 gcatccgggc cacgggtgag acaatcccag cccgtcgccc accgatcagg gcccgtttga
     3001 ccccgcccgt ttcccgcggc gctttgccac catgccgacc actgctgccc ccctgcgcct
     3061 gtcgaccgcg caacccgatt ttgaaacccg gttttcggcg cgcctgcact ggagcgccga
     3121 ccaggacgaa gccatcgagc agcgcgtcaa agccatcctg gccgacgtgc gcgcgcgcgg
     3181 cgacgcggcg gtgctggagt acaccgcgcg cttcgacggc ctgcaggccg cgtcgatggc
     3241 cgaactggaa ctgaagcaga gcgacttcaa ggccgctttc gacagcctgc ccgccgagca
     3301 gcagcaggcg ctgcaggagg ccgccgcgcg cgtacgccgc taccacgagt ggcagaaaaa
     3361 gcagggcggc gaaaccgcga cctaccgcga cgacgacggc acgctgctcg gccagaaggt
     3421 cacgccgctc gaccgcgtgg gcatctacgt gcccggcggc aaggcggcct acccgagcag
     3481 cctgatcatg aacgccgtgc ccgcgcacgt ggccggcgtg ggcgagatca tcatggtggt
     3541 gccgacgccg gtgcgcggca gcatcgccac cggtggttcc gattcaaaac agcccccggc
     3601 gcttgatggg caagcgccag cagctattga ttcaggagtg gccggcgagc gcaacccgct
     3661 ggtgctggcc gccgcgcacg tcgccggcgt cacgcgcgcc ttcaccatcg ggggtgcgca
     3721 ggccatcggc gcgctggcgt atggcacggc caccatcccc aaggtcgaca agatcaccgg
     3781 ccccggcaac gcctacgt
//
LOCUS       DAOMVO010000242         3797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7007_length_3797_cov_11.486104, whole genome shotgun sequence.
ACCESSION   DAOMVO010000242 DAOMVO010000000
VERSION     DAOMVO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7007_length_3797_cov_11.486104"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKV30_10670"
     CDS             <1..428
                     /locus_tag="PKV30_10670"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRL30707.1"
                     /translation="YLMGVGTPEDLVYGVQQGVDMFDCVMPTRNARNGHLFTRFGDLK
                     IRNARHKTDPRPIDETCTCYACAGASGVAYSQGGREGFSRAYLHHLERCGEMLAPMLA
                     TVHNLHYYLHLMSEVRAALDAGQLSAFAAQFHTDRARGV"
     gene            516..2255
                     /locus_tag="PKV30_10675"
     CDS             516..2255
                     /locus_tag="PKV30_10675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRL30708.1"
                     /translation="MSKSTPRRAPCWRSVAALAASAGLVVAAWAQTPAPAAGERSAVP
                     VAASTSTAPPAPSATPSAATRPPDASEIWAALQKAGVKESEVALLAQPLAPGAPLLIS
                     HNEKLAFTLASTTKVITTLASLQTLPGSFRWRTRAWLTGTPQDGRLTGDFILVGGGDA
                     TLDSKRLTDWFKRLRKQGLNHVQGQIVLDQSLFRLAAKDHANTPVPEAGNPHHAWPDA
                     FVLDDSRFQVKLRAGDSGALAATFDPPLGQVEVVSQISGSRLCTAQARFEPAQDKPAA
                     KPAPTPEVALSPMVPVSLPAASAASAPEQAASSPEPAAVAVKPTPPPPVGRVIVSGQW
                     SPRCVNALTMEAAPGRALTAALVAGAWAAAGGQLDGRVLHRDGAAPTVRGARRVRTPA
                     SPYPGKPWATLDSAPLPELVRAINKDSNNLMARNLLLSMAPGFSPTTSTLDLARQRLT
                     LWFKKVGVAASAPKVDNGSGLAHSEQGSVLAMVQLLQTAWGDPKTQKVFLASLPVAGA
                     DGTLAGRFKGSPAQRNAFLKTGTLTGVRSLAGYVRGQSGQMIAVAIVVNSEQAGAAVP
                     ALDKFIEWVRQHG"
     gene            2299..>3797
                     /locus_tag="PKV30_10680"
     CDS             2299..>3797
                     /locus_tag="PKV30_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRL30709.1"
                     /translation="MRFSVPTFFTSAAPGALARRFFFALSLTGLLAAAPAHAQEGAAE
                     LLTAIKSRAASAVASPASDAAPPPADAKLVQRATRATQQDDERMLADITARLSRNPAF
                     AGVQPTVQAGVAQLTGHVPDNEARQLAAKIVGAIEGVVAVDNQLTLQTNFSARLAETW
                     VIAKDRLVRTVALVPLLLMAAVVVWLANLLGRWLSNHLGILRLDNRNPYLGTLVRRAV
                     RTVFVLGGVLVALDLLEATALVTAVLGSAGVVGIVLGFAFRDMAENYLSGILLSLRRP
                     FEPGDYVRVDSYEGRVVSLSTRNTVMMTLDGNELRLPNATVFKAVLLNFTHNPRRRFT
                     FKLGVAYGEDLRRAQDLGVSTMAAMQGVLADPGPSASIEAVGDSAVTINYFAWVDQRQ
                     TDFVKARSEAIRLVKTALEDAGMDLPEPIYRVQLTLPEGHDADTAAAALPPVLAPLVA
                     PTPAAAAPAPVDPQAVVRTQGDVSPNTELDAQIAQARARKAGQDMLQTPG"
BASE COUNT          557 a         1376 c         1306 g          558 t
ORIGIN      
        1 gctacctgat gggcgtgggc acgccggaag acctggtcta cggcgtgcag cagggcgtgg
       61 acatgttcga ctgcgtgatg cccacgcgca acgcgcgcaa cggccatttg ttcacgcgct
      121 tcggcgactt gaagattcgc aatgcgcgcc acaagaccga cccgcgcccg atcgacgaga
      181 cctgcacctg ctacgcctgc gccggcgcgt ctggcgtggc gtattcgcaa ggcgggcgcg
      241 aaggtttcag ccgtgcctac ctgcatcact tggaacgctg cggcgagatg ctggcgccca
      301 tgctggccac ggtgcacaac ctgcattact acctgcattt gatgagcgaa gtgcgcgcgg
      361 cgctcgacgc gggccaactg agcgccttcg ccgcgcaatt tcacacggat cgcgcgcgcg
      421 gcgtgtaggc gcggcgcgcc ccgtgcgcat ttcagcgttt acaagcaaaa ttggcctccg
      481 gcgcttgacc aacaagcgca agaagctatc aattcatgag caaatcaacc ccccgtcgcg
      541 cgccgtgctg gcgcagcgtg gccgcgctgg ccgcgtcggc tgggctggtc gtggccgcgt
      601 gggcgcaaac gcctgcgccg gcggcgggtg agcgctcggc ggtgccggtc gccgcttcca
      661 ccagcaccgc gccgcccgcg cccagcgcca caccgagcgc cgccacccgc ccgcccgatg
      721 cgtcggaaat ttgggcggcg ctgcaaaaag ccggcgtcaa ggaatccgaa gtcgccctgc
      781 tcgcgcagcc gctggcgccg ggcgcgccgc tgctgatctc gcacaacgag aagctggcct
      841 tcaccctggc ttcgaccacc aaggtcatca ccacgctggc cagcctgcag acgctgcctg
      901 gcagctttcg ctggcgcacg cgcgcctggc tgaccggcac gccgcaggat ggccggctga
      961 cgggcgattt catcctggtc ggcggcggcg acgccacgct cgacagcaag cgtctcaccg
     1021 actggttcaa gcgcttgcgc aagcagggct taaaccacgt gcagggccag atcgtgctcg
     1081 accaaagcct gttccggctg gcggccaagg accacgccaa cacgcccgtg cccgaagccg
     1141 gcaacccgca ccacgcgtgg cccgacgcct tcgtgctgga cgacagccgc tttcaggtca
     1201 agctgcgcgc gggcgacagc ggcgcgctgg ccgccacctt cgatccgccg ctggggcaag
     1261 ttgaagtcgt cagccagatc agcggcagcc gcctgtgcac ggcgcaggca cggttcgagc
     1321 cggcgcaaga caaacccgcc gccaagcccg caccaacgcc cgaagtcgcg ctgtcgccca
     1381 tggtgccggt gtcgctgccg gccgcatcgg cggccagcgc gccggagcag gcggcatcca
     1441 gccccgaacc tgcggcggtg gccgtcaagc cgacgccacc accgccggtg ggccgcgtca
     1501 tcgtcagcgg ccagtggtcg ccgcgctgcg tgaacgcgct gacgatggaa gccgcgcccg
     1561 gccgcgcgct gacggccgcg ctggtcgcgg gcgcctgggc cgccgccggc ggtcaactgg
     1621 acggccgcgt gctgcaccgc gacggcgccg cgcctacggt gcgcggcgcg cggcgcgtgc
     1681 gcacgcccgc cagcccctac cccggcaagc cgtgggccac gctcgattcg gcgccgctgc
     1741 ccgagttggt gcgcgccatc aacaaggaca gcaacaacct gatggcgcgc aatctgctgc
     1801 tgtcgatggc gccgggtttt tcgcccacca cctccacgct cgatctggcc cggcagcggc
     1861 tgacgctgtg gttcaagaag gtgggcgtgg ccgccagcgc gcccaaggtc gacaacggtt
     1921 ccggcctggc gcacagcgag cagggcagcg tgctcgccat ggtgcaactg ctgcaaacgg
     1981 catggggcga cccgaagacg cagaaggtgt tcctggcctc gctgccggtg gcgggtgccg
     2041 acggcacgct ggccggccgc ttcaagggct cgccggcgca gcgcaacgcc tttctgaaaa
     2101 ccggcacgct gaccggcgtg cgctcgctgg cgggctatgt gcgcggccaa tcgggccaga
     2161 tgatcgccgt cgccatcgtc gtcaacagcg agcaggccgg cgccgccgtg ccggcgctgg
     2221 acaagttcat cgagtgggtt cgccagcacg gctgagggcg gcgggccggc ccgctgccgc
     2281 ttgcggcatc attgggcgat gcgattttcg gtaccgacgt ttttcacttc cgccgcgccg
     2341 ggcgcgctcg cccggcgctt cttttttgct ctgtcactga ctggcctgct cgccgccgcc
     2401 cccgcgcacg cgcaggaagg tgccgccgaa ctgctcaccg ccatcaagtc gcgcgccgcc
     2461 agcgccgtgg cgtcgcccgc gtccgacgcc gcgccgcctc cggccgatgc caagctggtg
     2521 cagcgcgcca cccgcgccac gcagcaggac gacgagcgca tgctggccga catcaccgcg
     2581 cggctgtcgc gcaacccggc ctttgccggc gtgcagccca cggtgcaggc cggcgtggcg
     2641 cagctcaccg gccacgtgcc tgacaacgag gcgcgccagc tggcggccaa gatcgtcggc
     2701 gccattgaag gcgtggtggc ggtcgacaac cagctcacgc tgcagaccaa tttcagcgcc
     2761 cgcctggctg aaacctgggt catcgccaag gaccgcctgg tgcgcaccgt ggcgctggtg
     2821 ccgctgttgc tgatggcggc ggtggtggtg tggctggcca acctgctggg gcgctggctg
     2881 tccaaccacc tgggcattct gcggctcgac aaccgcaacc cctacctcgg cacgctggtg
     2941 cggcgcgccg tgcgcaccgt gttcgtgctg ggcggcgtgc tggtggcgct cgacttgctg
     3001 gaagccaccg cgctggtcac cgccgtgctc ggctcggccg gcgtggtcgg catcgtgctg
     3061 ggctttgcgt ttcgcgacat ggccgagaac tacctgtcgg gcattctgct cagcctgcgc
     3121 cggccgtttg agccgggcga ttacgtgcgg gtcgattcgt acgaaggccg cgtggtgtcg
     3181 ctgtcgaccc gcaacacggt gatgatgacg ctcgacggca acgagctgcg gctgcccaac
     3241 gccaccgtgt tcaaggccgt gctgctcaac ttcacgcaca acccgcgccg gcgcttcacc
     3301 ttcaagctgg gcgtggccta tggcgaagac ctgcgccgcg cgcaggacct gggcgtatcg
     3361 accatggccg ccatgcaggg cgtgctggcc gacccggggc ccagtgcgtc gatcgaggcg
     3421 gtgggcgatt cggccgtcac catcaactac ttcgcctggg tcgatcagcg gcagaccgat
     3481 ttcgtcaagg cgcgcagcga ggcgattcgg ctcgtcaaga cggcgctgga agacgccggc
     3541 atggatttgc ctgagccgat ctaccgcgtg cagctcacgc tgcccgaagg ccacgacgcc
     3601 gacaccgctg ctgccgcgct gccgcccgtg ctggcgccgc tggtcgcgcc gacgcccgcc
     3661 gctgcggcgc ccgcgccggt cgacccgcag gccgtggtgc gcacgcaggg cgatgtgtcg
     3721 cccaacaccg agctggacgc gcagattgcg caggcgcgtg ctcgcaaggc ggggcaggac
     3781 atgttgcaga cccccgg
//
LOCUS       DAOMVO010000243         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7093_length_3762_cov_13.131913, whole genome shotgun sequence.
ACCESSION   DAOMVO010000243 DAOMVO010000000
VERSION     DAOMVO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7093_length_3762_cov_13.131913"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKV30_10685"
     CDS             <1..575
                     /locus_tag="PKV30_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL30710.1"
                     /translation="SDAKDAKDFIAKAKAAPGKISYGSAGAGSPHHLAMEMLKSRTGV
                     HMVHIPYRGAAPALADLAGGQIPVMMVDMAAGAAFIKSGKIRPLAVANDKRLPQLPDV
                     PTFDELGMKGVEAAALVGLVAPAALPADVKATLQKQVAAAIEQPAVKQKLIEFGVEPV
                     GSSSQAYADLLKAENARWHKLIKDLKITLD"
     gene            complement(657..2000)
                     /locus_tag="PKV30_10690"
     CDS             complement(657..2000)
                     /locus_tag="PKV30_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRL30711.1"
                     /translation="MKSIAQIAAELQGYDPQALSADAVNAFLERLVEPVTQTERVPLF
                     SALGRVLAEDIVSPISVPPHDNSAMDGFAFDGAQLLPGQPLSLKVVGTALAGAAWRGH
                     VARGECVKIMTGAVMPAGLDTVVPQEFTRVDGDTVTVPANVVKLGDNRRKLGEDLMAG
                     QPALQKGELLAPAALGLMASLGMAEVSVMRRLRVAYFSTGDEILSLGEPPREGAVYDS
                     NRYTVFGLLTRMGCEVIDLGVVKDQPALLEAAFLHAAREADAVITSGGVSVGEADHTK
                     AMMKKLGDVAFWRIAMRPGRPMAVGRIAVPDLQGESASGARRTSASSSQNDRNPAILF
                     GLPGNPVAVMVTFFAFVRPALLRMMGARAEAPLLLRAASETPMRKKPGRTEYQRGIVT
                     QHADGRLTVRTTGNQGSGVLSSMAQANGLIVLGHGQGDVAVGDQVSVMMFEGAVG"
     gene            complement(2077..2595)
                     /locus_tag="PKV30_10695"
     CDS             complement(2077..2595)
                     /locus_tag="PKV30_10695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRL30712.1"
                     /translation="MPADVTIRLLTDTDLPAYKALRDIGLQHDPEAFTSDFDTASALP
                     PATYATRLGQPPDDHFILGAFDSSGAMLGAVVCQRQAHLKQRHEAELVGMIIAPAARS
                     KGIGKALLREFDTLVRKLPGLQQVVLSVTASNAAAVRLYEGAGFVRYGLQPRAVQVDG
                     VYHDKALMVKQL"
     gene            complement(2612..3241)
                     /gene="mobA"
                     /locus_tag="PKV30_10700"
     CDS             complement(2612..3241)
                     /gene="mobA"
                     /locus_tag="PKV30_10700"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HRL30713.1"
                     /translation="MTSDITALLLAGGRGSRMGGVDKGLQNFNGTPLAMNALLRLSQQ
                     TLPPAEVMINANRNLSAYESFGAPVWPDALPDYAGPLAGFLTGLEQCETPLLLTLPCD
                     VPNFPLSLCERLAHALLAQEAEIAMVSAPEADDSGATRLRPQPVFCLLRAELLESLVR
                     FTQAGGRKIDAWTAQHRQVLVPFNEPSDDPLAFANANTLAELRALEQHR"
     gene            complement(3247..3699)
                     /locus_tag="PKV30_10705"
     CDS             complement(3247..3699)
                     /locus_tag="PKV30_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30714.1"
                     /translation="MALSPDTAANLFPLNGVSAASNSSVPHSFYIQKRAVVAHLVAQG
                     IELLGKSDLLWTGSATPQTLTQIGHDLVKLRQVVAPHLDSRILGTREVQQSLADLNAL
                     FRRHALRYPDFLEHLFAPPEIPDCAPLLQQIRLSLRLAKKHHVWQLPQ"
BASE COUNT          664 a         1214 c         1240 g          644 t
ORIGIN      
        1 gctccgacgc caaggacgcc aaggacttca tcgccaaggc caaggccgcg ccgggcaaga
       61 tcagctacgg ctcggccggc gcgggcagcc cgcaccacct ggccatggaa atgctgaagt
      121 cgcgcaccgg cgtgcacatg gtgcacatcc cctaccgcgg cgcggcgccc gcgttggccg
      181 acctggcagg cgggcagatt cccgtgatga tggtggacat ggccgccggc gcggccttca
      241 tcaagtccgg caagatccgc ccgctggccg tggccaacga caagcgcctg ccgcagctgc
      301 ccgacgtgcc cacgttcgat gaactgggca tgaaaggcgt tgaagccgcc gcgctggtcg
      361 gcctggtcgc gcccgccgcg ctgccggccg atgtgaaagc cacgctgcaa aagcaggtgg
      421 cggcggccat cgagcaaccg gccgtcaagc agaagctgat cgagtttggc gtcgaacccg
      481 tcggcagttc atcgcaggcc tacgccgatc tgctgaaggc cgagaacgcg cgctggcaca
      541 agctgatcaa ggatttgaaa atcacgctgg attgagacga tggcccgttg tgggagcggc
      601 cttggcagcc atgggccgtc atcgcgggca accccgctcc catcattcgc tgagcgttaa
      661 ccgaccgcgc cctcgaacat catcacgctg acctgatcgc ccacggccac gtcgccctgc
      721 ccgtggccca gcacgatcag gccgttggct tgcgccatgg acgacagcac gcctgagcct
      781 tgattgccgg tggtgcgcac cgtcaggcgg ccatcggcat gctgggtgac gatgccgcgc
      841 tggtattcgg tgcgaccggg ctttttgcgc atgggcgttt cgctggcggc gcgcagcagc
      901 aagggcgctt cggcacgcgc acccatcatg cgcagcaggg ccgggcgcac gaaggcaaag
      961 aaggtcacca tcaccgccac cgggttgccg ggcaggccga acaggatggc gggatttcta
     1021 tcattttgag agctgctcgc gcttgtccgg cgtgcgccag aggctgattc accttgtaaa
     1081 tcaggcacgg cgatgcgccc caccgccatc ggtcggccgg ggcgcatggc gatgcgccag
     1141 aaggcgacgt cgccgagttt tttcatcatc gccttggtgt gatccgcctc gccgacgctg
     1201 acgccgccgc tggtgatgac cgcatcggct tcgcgcgcgg cgtgcaggaa ggcggcttca
     1261 agcagcgcgg gctggtcctt caccacgccc aggtcgatca cttcgcaacc catgcgcgtg
     1321 agcaggccga acacggtgta gcggttgctg tcgtacacgg cgccttcgcg cggcggctcg
     1381 cccaggctaa gaatttcatc gccggtggag aagtaagcca cgcgcaggcg gcgcatcaca
     1441 gagacttcgg ccatgcccag actggccatc agcccaagcg cagcgggcgc cagcagctct
     1501 cctttttgca gcgcgggctg gccggccatc aggtcttctc ccagcttgcg gcggttgtcg
     1561 ccgagcttga ccacgttggc gggcacggtg accgtatcgc catccacgcg cgtgaattcc
     1621 tgcggcacca ccgtgtccag gccggcgggc atgacggcgc cggtcatgat cttcacgcac
     1681 tcgccgcgcg ccacgtggcc gcgccaggcg gcgccggcca gcgcggtgcc gacgactttc
     1741 aatgacaaag gctggccggg cagcagttgc gcgccgtcaa acgcaaagcc gtccatggcc
     1801 gagttgtcgt gcggcggcac gctgatgggc gagacgatgt cttcggccag cacgcggccc
     1861 agcgcgctga acagcggcac gcgctcggtc tgcgtgacgg gttcgaccaa gcgctcaaga
     1921 aacgcgttca ccgcatcggc ggacaacgct tgcgggtcgt agccttgcag ttcggcggcg
     1981 atctgggcga tcgacttcat ggtggtggcg tgatgtgagg tttcaagaca aaaaggccgc
     2041 cagcgcttgt ccaccaagcg cgagcagcta tcaatatcaa agctgcttca ccatcagcgc
     2101 cttgtcgtgg taaacgccat cgacctgcac ggcgcgcggc tgcagaccat agcgcacaaa
     2161 gcccgcgccc tcatacaggc gcaccgccgc ggcgttgctg gcggtgacgc tcagcaccac
     2221 ctgctgcagc cccggcagct tgcgcaccag cgtatcgaac tccctcaaca acgccttgcc
     2281 gatgcctttg ctgcgcgccg cgggcgcgat gatcatgccg accagttcgg cctcgtggcg
     2341 ctgcttcaaa tgcgcctggc gctggcacac cacggcgccc agcatggcgc cgcttgaatc
     2401 gaaggcgccc aggatgaagt ggtcatccgg cggctggccc aggcgcgtgg cgtacgtcgc
     2461 cggcggcaag gcgctggcgg tatcgaaatc gctggtgaag gcctcggggt cgtgctgcag
     2521 gccgatatcg cgcagcgcct tgtaagcggg caggtcggtg tcggtcagca ggcggatggt
     2581 gacgtcggcg ggcatgggcg tgtctccttt ctcatcggtg ctgctccagc gcgcgcagct
     2641 cggccagcgt gttggcattg gcaaaggcca gcgggtcatc gctaggttcg ttgaagggca
     2701 ccagcacctg cctgtgctgc gccgtccagg cgtcgatctt gcggccgccg gcctgcgtga
     2761 agcgcactag gctctcgagc agctcggcgc gcagcaggca gaacaccggc tgcgggcgca
     2821 gccgcgtggc gccgctgtcg tcggcttcgg gggccgacac catggcgatt tcggcctctt
     2881 gcgcaagcag ggcgtgcgcc aggcgctcgc aaagcgatag cgggaagttg ggcacgtcgc
     2941 acggcagggt gagcagaagc ggcgtctcgc actgctccag cccggtcaga aagccggcca
     3001 gcggccccgc gtagtccggc agcgcatcgg gccacaccgg cgcgccgaac gattcatagg
     3061 ccgacaggtt gcggttggcg ttgatcatta cctcggcggg cggcaaggtt tgctgcgaaa
     3121 ggcgaagcag cgcgttcatc gccagcggcg tgccgttgaa gttctgcaag cccttgtcga
     3181 ccccgcccat gcggctgccg cgcccgccgg cgaggaggag ggcggtgatg tccgacgtca
     3241 tgtgatttac tgaggaagtt gccatacatg atgtttcttg gctaatcgaa gcgatagtct
     3301 gatctgctga agaagtggtg cgcagtccgg tatttccgga ggcgcgaata aatgctcaag
     3361 gaagtcagga tatctaagcg catgtcgtct gaagagcgca tttagatccg ccaaagattg
     3421 ctgcacttcc cgggttccca aaatgcgact gtcgagatgc ggggcgacga cttggcgcag
     3481 cttgactaag tcgtgaccaa tctgcgtgag cgtctgtgga gttgccgatc cagtccaaag
     3541 cagatcgctt ttgccgagca actctatccc ctgagcgacc aaatgggcaa ctacagcccg
     3601 cttttgtata tagaaggagt gcggcaccga tgaattgctt gccgcgctca caccattcaa
     3661 cggaaacaaa tttgcggccg tatccggtga caacgccatt tgcgctcgcg atatctgcat
     3721 gggtaggcat tgcatctacc ccccgatgta actcatctcc ac
//
LOCUS       DAOMVO010000244         3755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7117_length_3755_cov_15.169730, whole genome shotgun sequence.
ACCESSION   DAOMVO010000244 DAOMVO010000000
VERSION     DAOMVO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_3755_cov_15.169730"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKV30_10710"
     CDS             <1..388
                     /locus_tag="PKV30_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRL30715.1"
                     /translation="NQAAQIWNHTFFWNCMKPNGGGEPTGALAEAINKKWGSYADFKK
                     AFVTSAVGNFGSGWTWLVKKPDGSVDIVNMGAAGTPLTTGDTAVFTVDVWEHAYYIDY
                     RNLRQKFVETYLDKLANWEFAAKNFG"
     gene            complement(625..1689)
                     /gene="cydB"
                     /locus_tag="PKV30_10715"
     CDS             complement(625..1689)
                     /gene="cydB"
                     /locus_tag="PKV30_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRL30716.1"
                     /translation="MNPADLVLTAADGAAFWLPVAWLGVIGFGVLMYVLLDGFVLGLG
                     ILAPLAEDSDQLDHMMNTAAPIWDGNETWLVLGGAGLMAAFPRAYALVLSSLYLPVLA
                     MLLGLIFRGVAFEFRFKGRGVARQAWGAAFSIGSIVAAFSQGVILAALVQGLPMEGGH
                     YSGGTWGWLSPFALMTGVALVCGYALLGAGWLILKTEGRLNQLARGWARPLVVCVLAF
                     MGLVSAALPYLDSRVMARWFEGSNFLWLGPVPLLTLAVGWWLWRDAAIGQGADRPHDA
                     KPFVWTLALFVLGLVGLLLGLWPYLVPPHLTIWGAASPPESQGFVMWGLVFLLPLILS
                     YTAWSYSVFRGKVGADAGYH"
     gene            complement(1686..3107)
                     /locus_tag="PKV30_10720"
     CDS             complement(1686..3107)
                     /locus_tag="PKV30_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRL30717.1"
                     /translation="MDALLLSRIQFGFVISYHILFPAFTIGLASWLMFLEFRWLRTRD
                     NVWWDLYKFWSKVFAISFGMGVVSGIVMSFQFGTNWSRLSERAGAVLGPLLSYEVLTA
                     FFLEAGFLGVMLFGANKVRPLVHFFCTCMVALGTLISTFWIIAAISWMHTPQGHVLKD
                     GFFTPGDWWQIVFNPSFPYRLTHMVLAAFITTCFVIGGTAAWHLRRGVQREMALRVLK
                     LSIAFGAIAVPMQVMVGDQHGLNTLEHQPIKVAAMEGHWHRGEPGAGVPLVAFAIPNQ
                     QAERNDYEIAIPRLGSLILTHSLDGEITPLTAVAPQDRPPVLPVFFAFRVMVGIGMLM
                     LLVVAFSCWTWWRGRLEQSVWMLRGWQLLSPAGFVALLAGWWVTEIGRQPWVVYGLMR
                     TADAVSPNLQASTVATSLAGYATVYAIIFGAGIWYLWRLLKKGPQAGETPPEMGTPDS
                     GAKTPARPLSAADEADIAEGARP"
     gene            3292..>3755
                     /locus_tag="PKV30_10725"
     CDS             3292..>3755
                     /locus_tag="PKV30_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HRL30718.1"
                     /translation="MGFKPLPAAVAVAIALIIWFVIPVPAGVTANAWHLLALFVATIA
                     AIIGKAMPIGALSIVAIALVAVTGVTNATPGGAIADALSSFANPLIWLIGVSIMISRG
                     IIKTGLGARIGYLFIAVWGKKTIGIAYSLALSELILAPVTPSNTARGGGIIHP"
BASE COUNT          741 a         1309 c         1188 g          517 t
ORIGIN      
        1 caaccaggcc gcgcagatct ggaaccacac cttcttctgg aactgcatga agccgaatgg
       61 cggcggcgag ccgaccggcg cgctggccga ggcgatcaac aagaagtggg gcagctacgc
      121 cgacttcaaa aaggcgtttg tcaccagcgc tgtcggcaac ttcggctcgg gctggacgtg
      181 gctggtgaaa aagccggacg gctcggtcga catcgtcaac atgggcgccg ccggcacccc
      241 gctgaccacc ggcgacaccg ccgtgttcac ggtcgacgtg tgggaacacg cgtactacat
      301 cgactaccgc aacctgcgcc agaagtttgt cgaaacctac ctcgacaagc tggccaactg
      361 ggaattcgcg gccaagaact tcggctgatc ggccgtcttt tcgccccaca aaaatgccac
      421 cggattcggt ggcatttttc atggccaaat cggccgccag cgcttgtcca tcaagcgcca
      481 gcagctatca aaaccaaagt ggctgcgcag caagccaccg aaagggccgc ggagcaggcc
      541 acccgagaaa gccgtgcgcg gggtcccccc gcgcgccagc gttgtccccc tggggggaaa
      601 gcgccgcagg cgactcaggg gggatcagtg atatccggcg tccgccccca ccttgccccg
      661 aaacacgctg tacgaccagg cggtgtaact caggatcagc ggcagcaaga agacgagtcc
      721 ccacatcacg aagccttgcg attcgggcgg cgaggcggcg ccccagatcg tcaggtgcgg
      781 cggcaccagg tagggccaca ggcccagcag caggccgaca aggcccagca cgaacagcgc
      841 cagcgtccag acaaagggct tggcatcgtg cggccgatcg gcgccctggc caatggcggc
      901 gtcgcgccac agccaccagc cgacggccag cgtcagcaag ggcaccggcc ccagccacag
      961 gaagttgctg ccttcaaacc agcgcgccat gacgcgtgaa tccaagtagg gcagcgccgc
     1021 gctgaccagc cccatgaagg ccagcacgca caccaccagc ggccgcgccc agccgcgcgc
     1081 caactggttc aggcggcctt cggtcttcaa gatcagccaa cccgcgccca acagcgcata
     1141 accgcagacc agcgccacgc cggtcatcag cgcgaacggg ctcagccagc cccaggtgcc
     1201 gccgctgtag tggccgcctt ccattggcaa gccctgcacc agcgcggcga gaatcacgcc
     1261 ctggctgaac gccgccacga tggagccgat gctgaacgcc gcgccccagg cctgccgcgc
     1321 cacgccgcgc cccttgaagc gaaattcaaa cgccacgccg cgaaagatca gccccagcag
     1381 catcgccagc accggcaaat acagcgacga cagcaccagc gcgtacgcgc gcgggaaggc
     1441 ggccatcaac ccggcgccgc ccagcaccag ccaggtctcg ttgccgtccc agatcggggc
     1501 ggcagtgttc atcatgtggt cgagctggtc gctgtcttcg gccagcgggg cgaggatgcc
     1561 caggccgagc acgaagccgt ccagcagcac gtacatcagc acgccgaagc cgatcacgcc
     1621 cagccacgcc accggcagcc agaaggcggc gccgtcggcg gccgtaagca ccaggtcggc
     1681 ggggttcatg ggcgcgctcc ttcggcgatg tcggcctcgt cggccgccga cagcgggcgc
     1741 gccggggtct tggcgccgct gtccggggtg cccatctcgg gcggcgtctc gccagcctgc
     1801 ggacctttct tgagcagccg ccacaaatac cagatgccgg cgccaaagat gatggcgtac
     1861 accgtggcgt agccggccag cgacgtggcg acggtgctgg cctgcaggtt ggggctgacg
     1921 gcgtcggcgg tgcgcatcag gccgtacacc acccacggct ggcggccgat ttcggtcacc
     1981 caccagccgg ccagcagggc gacgaagccg gccgggctga gcagttgcca gccgcgcagc
     2041 atccacaccg attgctccag ccgcccgcgc caccaggtcc agcacgaaaa agccaccacc
     2101 agcaacatca gcatgccgat gccgaccatg acgcgaaagg caaagaacac cggcagcacc
     2161 ggcggccggt cttgcggcgc cacggcggtc agcggggtga tctcgccatc caggctgtgc
     2221 gtcaggatca ggctgcccag gcgcgggatg gcgatttcgt agtcgttgcg ctcggcctgc
     2281 tggttgggga tggcaaaggc caccagcggc acgccggcgc cgggctcgcc acggtgccag
     2341 tggccttcca tcgccgccac cttgatcggc tggtgctcca gcgtgttcag cccgtgctgg
     2401 tcgcccacca tcacctgcat cggcacggca atggcaccaa aggcaatcga cagcttgagc
     2461 acccgcagcg ccatctcgcg ctgcacgccg cgccgcaggt gccaggccgc cgtgccgcca
     2521 atcacgaaac aggtggtgat gaaggccgcc agcaccatgt gcgtgaggcg ataggggaac
     2581 gaggggttga acacgatctg ccaccagtcg ccgggcgtga aaaagccgtc tttcagcaca
     2641 tggccttgcg gcgtgtgcat ccagctgatg gcggcaatga tccagaaggt ggagatcagc
     2701 gtgcccagcg ccaccatgca ggtgcagaaa aagtgcacca gcggccgcac cttgttggcg
     2761 ccaaacagca tcacgcccag aaagccggcc tcgagaaaga aggccgtcag cacctcgtag
     2821 ctgagcagcg gccccagcac ggcgccggcc cgctcggaca ggcgcgacca gttggtgccg
     2881 aactgaaagc tcatcacgat gcccgacacc acccccatgc cgaaggagat ggcgaacacc
     2941 ttgctccaga acttgtacaa gtcccaccac acgttgtcgc gcgtgcgcag ccagcggaat
     3001 tccagaaaca tcagccagct tgccaacccg atggtgaagg ccgggaacag gatgtgatac
     3061 gaaataacga acccgaactg aatccgggag agcagcaggg cgtccatggc gcgagtttgc
     3121 gcgcggtggg cgcatacgct tgtaggtggg cgcctccagt cgcctgaaac tcttgacaac
     3181 ggtcaagacg cgttggcacg ccgcatgcaa tgatggcgat gcactgcgcg tcatgaaaac
     3241 gtcacgcgaa tcgcatccga tgcgcattgc tgccaagaca aggaacaccg catgggcttc
     3301 aagccgcttc ccgcagccgt cgccgtggcg atcgcgctga tcatctggtt cgtcattccg
     3361 gtgcctgcgg gcgtcaccgc caacgcctgg cacctgctgg cgctgttcgt ggcgaccatc
     3421 gccgccatca tcggcaaggc gatgccgatc ggcgcgctgt ccatcgtcgc catcgcgctg
     3481 gtggcggtca cgggtgtcac caacgccacg ccgggcggcg ccattgccga tgcgctgtcg
     3541 agctttgcca acccgctgat ctggctgatt ggcgtgtcga tcatgatctc gcgcggcatc
     3601 atcaagaccg gcctgggcgc gcgcatcggc tacctgttca tcgccgtgtg gggcaagaag
     3661 accatcggca tcgcctattc gctggcgctg tcggagctga tcctggcgcc cgtcacgccc
     3721 agcaacacgg cgcgcggcgg cggcatcatc caccc
//
LOCUS       DAOMVO010000245         3754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7123_length_3754_cov_13.435253, whole genome shotgun sequence.
ACCESSION   DAOMVO010000245 DAOMVO010000000
VERSION     DAOMVO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7123_length_3754_cov_13.435253"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1579
                     /gene="thrC"
                     /locus_tag="PKV30_10730"
     CDS             152..1579
                     /gene="thrC"
                     /locus_tag="PKV30_10730"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRL30719.1"
                     /translation="MNYLSTRGDATPRRFCDILLEGLAPDGGLYLPEHYPQVDRMTLD
                     HWRHLYAQHGYATLAFEVLSLYIDDIPAAELKALCEKTYTPEVFGTLEIVPLKKLGDG
                     FYLEALSNGPTLAFKDMAMQLLGNLFEYELKRRGAQLNILGATSGDTGSAAEYAMRGK
                     QGIRVFMLSPEGRMSAFQQAQMFSLQDENIHNIAIEGVFDDCQDIVKAVSNDLEFKRR
                     HKIGTVNSINWARLLAQVVYYFAGYFQATRSNDERVDFTVPSGNFGNVCAGHVARQMG
                     LPIDQLVVATNENDVLDEFFRTGVYRVRGSADTHETSSPSMDISKASNFERFVFDLLG
                     RDGAKTKALFHDALLRDGQFDLSGEPAFSQARERYGFVSGKSTHADRLATIRDVFEEH
                     GVMIDTHTADGVKVAREHLQPGVPMIVLETALPIKFAVTIREALGREPDRPPRFDGIE
                     NLPRRVRVLPADAAQVQQLIVRECE"
     gene            1651..2202
                     /gene="mobB"
                     /locus_tag="PKV30_10735"
     CDS             1651..2202
                     /gene="mobB"
                     /locus_tag="PKV30_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRL30720.1"
                     /translation="MKVAAFAGYSGSGKTHLIEQLIPALKLRGLRVSVVKHAHHKFDI
                     DHEGKDTFRHREAGAFEVVVASSKRLALMREFEREAHLSVHHLIAELHDGVDWVLVEG
                     FKSCDLLKVEVWRAASAKPARYPDDDFIVAIATDSPEALPAASLRPVLDLNQPDALAE
                     WLIENGARFEYDPERHLPVASHV"
     gene            2195..3448
                     /locus_tag="PKV30_10740"
     CDS             2195..3448
                     /locus_tag="PKV30_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRL30721.1"
                     /translation="MSDPARRPPLMPLDDALARLLALAAPLGRTDQVSTFDADGRVLA
                     QDVVSPLQVPPADNSSMDGYAVRAADVTAAGVELPVTQRIAAGQSGTALPPGSAARIF
                     TGAPMPAGADAVVMQEDTETLEGGARVRIQVVPAPGQWVRRAGEDVMRGATVLHKGER
                     LAPAGMGLAAGIGLNQLLVASRPRVALFSTGDELVMPGDVAPQDMKPGAIYNSNRFFL
                     RALLTRLGCEVSDLGIVPDNRAATLAALQSAAHGHDLILTSGGVSVGEEDHIKPAVEQ
                     LGALDLWQIAMKPGKPFAYGHVRRGEGDAAHFMGLPGNPVSSFVTFLLLVRPFLLKLQ
                     GATALAPAAMAMRADFSLPKADKRREFLRARRNAEGGLDLFGNQSSGVLTSAHWADGL
                     IDLPAGQTIAEGDVVRFIPLAELLA"
     gene            3445..3702
                     /locus_tag="PKV30_10745"
     CDS             3445..3702
                     /locus_tag="PKV30_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRL30722.1"
                     /translation="MKTITVRYFASIREALGTGNERVSTAAPDLHALRDELIARDGAY
                     GEALARGKAVRLSLDQTMVADESAPLADGAEVAFFPPVTGG"
BASE COUNT          643 a         1245 c         1231 g          635 t
ORIGIN      
        1 tggggcggcg gcaggaagat ctggacattt tgattgccgc caccgccagc gcgcacggcc
       61 tgacccttgt cacccgcaat acccgccact tcatcgacac cggcgtgccg ctcatcaacc
      121 cctggggcgg cgccgatgcc gccagcaccg aatgaactac ctctccaccc gtggcgacgc
      181 cacaccgcgc cggttctgcg acatcctgct tgaaggcctg gcgcccgatg gcggcctgta
      241 tttgcccgag cactacccgc aggtcgaccg catgacgctc gaccactggc gccatctgta
      301 cgcgcagcac ggctacgcca ccctggcgtt cgaagtcctg tcgctgtata tcgacgacat
      361 tcccgccgcc gaactgaaag cgctgtgcga gaagacgtac acgcctgaag tctttggcac
      421 gctggagatc gtgccgctta aaaaattggg cgatggtttt tatctggaag ccctgtccaa
      481 cggccccacg ctggccttca aggacatggc gatgcagctg ctgggcaacc tgttcgagta
      541 cgaattgaag cgccgtggcg cgcaactcaa catcctgggc gccaccagcg gcgacaccgg
      601 cagcgcggcc gagtacgcca tgcgcggcaa gcaaggcatc cgcgtgttca tgctcagccc
      661 cgaagggcgc atgagcgcgt tccagcaggc gcagatgttc agcctgcaag acgagaacat
      721 ccacaacatc gccatcgaag gcgtgttcga cgactgccag gacatcgtca aggcggtcag
      781 caacgatctg gaattcaagc gccgccacaa gatcggcacc gtcaattcga tcaactgggc
      841 gcggctgctg gcgcaagtcg tctactactt cgccggctac tttcaggcca cgcgcagtaa
      901 cgatgagcgg gtcgatttca ccgtgcccag cggcaacttc ggcaacgtct gcgccggcca
      961 tgtggcgcgg caaatgggtt tgccgatcga ccaactggtg gtggccacca acgaaaacga
     1021 cgtgctcgac gagttcttcc gcaccggcgt ttaccgtgtg cgcggcagcg ccgacacgca
     1081 cgaaaccagc agcccgtcga tggacatcag caaggccagc aacttcgagc gcttcgtgtt
     1141 cgatctgctc gggcgcgatg gcgcaaagac caaagcgctg ttccacgacg cgctgctgcg
     1201 tgacggccag ttcgacctga gcggcgagcc cgccttcagc caggcgcgcg agcgatacgg
     1261 tttcgtgagc ggcaagagca cgcacgccga ccgcctggcc accatccgcg atgtgtttga
     1321 agagcacggc gtgatgatcg acacccacac cgccgatggc gtgaaggtgg cgcgcgaaca
     1381 cctgcagcct ggcgtgccca tgatcgtgct ggagacggcg ctgcccatca agtttgccgt
     1441 caccatccgc gaggcgctgg ggcgcgagcc cgatcgtccg ccgcgctttg acggcatcga
     1501 gaatctgccg cgccgcgtgc gcgtgctgcc ggcggatgcg gcgcaggtgc agcagctgat
     1561 cgtgcgcgaa tgtgaatgaa ataggccgcc agcgcttgat ggataagcgc aagcagctat
     1621 tgttttggta gttttcgaga tgattcagac atgaaggtgg ccgcattcgc cggctattcc
     1681 ggctcgggca agacgcacct gatcgagcag ctcattccgg cgctcaagct gcgcggcctg
     1741 cgcgtgtcgg tggtcaagca cgcgcaccac aagttcgaca tcgatcacga aggcaaggat
     1801 acgtttcgcc accgcgaagc cggtgccttc gaggtcgtcg tggcttcgag caagcggctg
     1861 gcgctgatgc gcgagttcga gcgtgaggcg cacctgtcgg tgcaccacct gattgccgag
     1921 ttgcacgacg gcgtcgactg ggtactggtc gaaggcttca agagttgcga cctgctcaag
     1981 gtcgaggtct ggcgcgccgc gtcggccaag ccggcgcgct accccgacga tgatttcatc
     2041 gtcgcgattg ccaccgattc gcccgaggcg ctgccggcgg cctccttgcg cccggtgctg
     2101 gacctgaacc agcccgacgc gctggccgag tggttgatcg aaaacggtgc ccggtttgag
     2161 tacgatcctg aacgccatct gcccgttgcc agtcatgtct gatcccgccc gccgcccacc
     2221 gctgatgccg ctcgacgacg cgctggcgcg cctgctggcg cttgccgcgc cgctggggcg
     2281 caccgatcag gtcagcacgt ttgacgccga tggccgcgtg ctggcgcagg acgtggtgtc
     2341 gcccctgcag gtgccacccg ccgacaactc ttcgatggac ggctacgcgg tgcgcgcggc
     2401 cgatgtgacg gctgccgggg ttgaactgcc cgtcacccag cgcattgccg ctggccagtc
     2461 cggcaccgcg ctgccgcccg gcagcgccgc ccgcatcttc accggcgcgc ccatgcctgc
     2521 aggcgccgac gcggtggtga tgcaggaaga caccgagacg ctggagggcg gcgcgcgcgt
     2581 gcgcatccag gtcgtgccgg cgcccggaca atgggtgcgc cgggccggcg aagacgtcat
     2641 gcgcggcgcc accgtgctgc acaaaggaga gcgcctggcg cccgctggca tggggctggc
     2701 ggccggcatc ggtctgaacc agctgctggt ggcgtcacgc ccccgggtcg cgttgttttc
     2761 gaccggcgac gaactcgtca tgccgggcga cgttgcaccg caagacatga agccgggcgc
     2821 catctacaac agcaaccgct ttttcctgcg tgcgctgctg acacggcttg ggtgcgaagt
     2881 gagcgatctc ggcatcgtgc ccgacaaccg cgccgccacg cttgccgcgc tgcaatctgc
     2941 ggcgcacggg catgatttga tcctgaccag cggcggtgtc agcgtgggcg aagaagacca
     3001 catcaagccg gcggtggagc aactgggcgc gctcgacttg tggcagatcg ccatgaagcc
     3061 gggcaaaccc ttcgcctatg gccacgtgcg gcgaggcgag ggcgacgcag cgcacttcat
     3121 gggcctgccc ggcaacccgg tgtcgagctt tgtcaccttt ttgctgctgg tgcggccgtt
     3181 cctgctcaag ctgcagggcg ccaccgcgct ggcgccggcg gcgatggcca tgcgcgccga
     3241 tttctcgtta cccaaggcgg acaagcggcg cgaattcctg cgtgcccgcc gcaacgccga
     3301 gggaggtctc gatttgttcg gcaaccagag ttctggcgtg ctgacctcag cgcactgggc
     3361 cgatggcctg atcgacctgc cggccgggca gaccattgcc gaaggtgacg tggtgcgctt
     3421 cattccgctg gcggagttgc tggcatgaag acgatcacgg tgcgctattt cgcctcgatc
     3481 cgcgaggccc ttggcacggg caacgagcgt gtgtctaccg ctgcacctga cttgcacgca
     3541 ttgcgcgacg agttgatcgc gcgggacggc gcctatggcg aggcattggc gcgcggcaag
     3601 gcggtccgcc tgtcgctcga ccagaccatg gtggcggacg aatcggcgcc gctggccgac
     3661 ggggccgaag tcgccttttt cccgcccgtg acgggggggt gacagcgtgc cacgcgtttc
     3721 cattcagacg cccgacttcg acctgagcgc cgaa
//
LOCUS       DAOMVO010000246         3712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7241_length_3712_cov_18.874761, whole genome shotgun sequence.
ACCESSION   DAOMVO010000246 DAOMVO010000000
VERSION     DAOMVO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7241_length_3712_cov_18.874761"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKV30_10750"
     CDS             <1..588
                     /locus_tag="PKV30_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HRL30723.1"
                     /translation="GIDIPDVAGAVFSGTASGDWTLSCVRGQVRSITFVFNDGTIRTI
                     PEDREGNQQNNQQRDGLGWISDPHGIPCVSGERRSNAQQYLGSQALITAAGAGVASLI
                     ESDSGRMSYVGSDGSIGTVGISGQEAVGQILAGGVRDMSAWVNKLYGQAFAAVYVQPG
                     AKVAVHLEKPLAIDFDPEGRKVDHRAGESHALELD"
     gene            569..1018
                     /locus_tag="PKV30_10755"
     CDS             569..1018
                     /locus_tag="PKV30_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HRL30724.1"
                     /translation="MHSNLTNLARGLALALAVAVLGGCATSKEKLLTHGDRTMMDIWQ
                     QEAGDGGGAAGRNAGGQLLDARQSLRRPLTDADMQAAPAEQMRYTRTARNEVHRQFQR
                     LPNPDLVMYVYPHLAGTDPVPVPGYTTVFPLYQRIQYAMPGERVEDY"
     gene            1018..>3712
                     /locus_tag="PKV30_10760"
     CDS             1018..>3712
                     /locus_tag="PKV30_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HRL30725.1"
                     /translation="MRWKLPWPKPAALKLAASGAGDDEQPDGWQRHIEALRQAGIPEP
                     GSAVRGRKPATEADEQALYEVAPSFVELLPWVEFLPESRSMLLEDGQSVAAFFELVPL
                     GTEGREPGWLAHARDALENALQDSFDELDENPWVLQLYAQDEPSFDQYMQTLRDYVQP
                     RARGTAFTEFYLRFFGHHLRAVAKPGGLFEDTVVTRLRWRGQTRRVRMVVYRRATGQA
                     SRRGQTPEQMLNIVCDRLCGGLANAGIQARRMVAADVHDWLLRWLNPRPAMLGPSTED
                     RERFYALARYPDETEAGEIELASGRDFSQRLFFGQPRSDVEHGTWYFDGMPHRVLITD
                     RLRMPPGTGHLTGETRKGDAINTLFDQMPEDTLLCLTMVATPQDVLESDLNHLAKKAV
                     GETLASEQTLKDVHEARSLIGSAHKLYRGTLAFYLRGRDEAELDRRGLDLANVMLNAG
                     LQPVREDDEVAPLNSYLRWLPCCYNPGKDRRRWYTQLMFAQHAANLSPVWGRAQGTGH
                     PGITMFNRGGGPITFDPLNRLDRQMNAHLFLFGPTGSGKSATLNNLLNQVTAIYRPRL
                     FIVEAGNSFGLFSDFARRLGLTVNRVKLAPGSGISLAPFADARRLIETPSDVQTLDAD
                     ALDEDLPPDASAMEADEQRDVLGELEITARLMITGGEDKEEARMTRADRSLIRQCILD
                     AAEHCVAEKRTVLTRDVRNALRTRGQDPTLPEMRRVRLLEMADAMDMFCQGTDGEMFD
                     RDGTPWPEADITLVDLATYAREGYNAQLSIAYISLISTVNNIAERDQYLGRPIINVTD
                     EGHIITKNPLLAPYVVKITKMWRKLGAWFWLATQNIDDLPRAAEPMLNMIEWWICLSM
                     PPDEVEKIARFRELSPAQKALMLSARKEAGKFTEGVILSKSM"
BASE COUNT          664 a         1249 c         1238 g          561 t
ORIGIN      
        1 ggcatcgaca ttcccgatgt ggccggcgcc gtgttcagcg gcaccgcctc gggcgactgg
       61 acgctttctt gcgtgcgcgg ccaggtgcgc agcatcacgt tcgtcttcaa cgacggcacg
      121 attcgcacga tccccgaaga ccgcgaagga aaccagcaga acaaccagca gcgcgatggc
      181 ttgggctgga tcagcgatcc ccacggcatt ccttgcgtca gcggcgagcg gcgcagcaac
      241 gcccagcagt acctcggctc gcaggccctg atcaccgcgg ccggtgccgg tgtggcctcg
      301 ctcatcgaga gtgacagcgg ccgcatgtcc tatgtcggct cggacggctc catcggcacc
      361 gtgggcatca gcggccagga ggcggtcggc cagattctgg cgggcggcgt gcgggacatg
      421 tcggcctggg tcaacaagct ctacggccaa gcgttcgccg ccgtctatgt ccagcccggc
      481 gcgaaggtcg ccgtccacct cgaaaagccg ctcgccatcg acttcgatcc cgaaggccgc
      541 aaggtcgatc accgcgcagg agaaagccat gcactcgaac ttgactaacc tggcccgtgg
      601 cctggcactg gccctcgccg tcgcggtgct tggcggctgc gccaccagca aggaaaagct
      661 gctgacccac ggtgaccgca cgatgatgga catctggcag caggaggccg gggacggcgg
      721 tggcgcagcc ggacggaacg ccggcggcca actgctcgat gcgcgccaga gcctgcgtcg
      781 gccgctgacc gacgccgata tgcaggccgc acctgccgag cagatgcgct acacgcgcac
      841 cgcgcgcaac gaggtccatc gccagttcca gcgcctgccc aatcccgatc tcgtgatgta
      901 cgtgtacccg cacctggccg gcaccgatcc cgtgccggtg cccggctaca cgaccgtctt
      961 cccgctgtac cagcgcattc agtacgcgat gccgggtgag cgcgtggagg actactgatg
     1021 cgctggaaac ttccctggcc taagccggcc gcgctgaagc tggccgcatc cggcgctggt
     1081 gatgacgagc agccggacgg ctggcagcgc cacattgagg ccttgcgcca agccggcatc
     1141 cccgaacccg gctcggcagt ccggggccgc aaaccggcga cggaggccga cgagcaggcg
     1201 ctgtacgagg tcgcaccgtc cttcgtggaa ctgctgccct gggtcgagtt cttgcccgag
     1261 tcaaggtcca tgttgctgga ggacggccaa tcggtggcgg ccttcttcga gctggtgccg
     1321 ctggggaccg agggccggga accgggctgg cttgcccacg cccgcgacgc cttggagaac
     1381 gcgctgcagg acagcttcga tgagctggac gagaacccct gggtgctcca gctctatgcc
     1441 caggacgagc cgagtttcga ccagtacatg cagaccctgc gtgactacgt gcagccacgt
     1501 gcccgcggga cagcgttcac cgagttctac ctgcgcttct tcggccacca tctgcgtgcg
     1561 gtggccaagc ccggcggcct gttcgaggac acggtggtca cgcggctgcg ctggcgcggg
     1621 cagacgcggc gcgtgcgcat ggtggtctat cgacgtgcca ccgggcaggc aagccgccgc
     1681 ggccagacgc ccgagcagat gctgaacatc gtctgcgacc gcctgtgtgg cgggctggcg
     1741 aacgccggca tccaggcacg gcgcatggtc gcagccgacg tccacgactg gctgctgcgc
     1801 tggctcaatc cgcgccccgc gatgctcggg cccagtacag aggaccggga gcgcttctac
     1861 gcactggcgc gctacccgga cgagactgag gccggcgaga tcgaactggc gagcgggcgg
     1921 gatttcagcc agcggctgtt cttcggccag ccacgctccg acgtggagca cggcacctgg
     1981 tacttcgacg gcatgccgca ccgtgtgctg atcaccgacc ggctgcgcat gccgcccggc
     2041 acgggacatc taaccggcga gacccgcaag ggcgatgcga tcaacacgct gttcgaccag
     2101 atgccggaag acaccttgct ttgtctcacg atggttgcca cgccccagga tgtcctggaa
     2161 tcggatctga accacctggc gaagaaggcc gtgggcgaga cgctggcgtc ggagcagacg
     2221 ctcaaggacg tgcatgaagc ccgctcgctg atcggcagcg cgcacaagct ctaccggggc
     2281 acgctggcgt tctacctgcg cgggcgcgac gaggcggaac tggatcggcg cggcctggac
     2341 ctggcgaacg tcatgctcaa cgccggcttg cagccggtgc gcgaggacga cgaggtggcg
     2401 ccgctcaaca gctacctgcg ctggctgccg tgctgctaca accccggcaa ggatcggcgc
     2461 cggtggtaca cgcaactgat gttcgcccag cacgcggcaa acctgtcgcc ggtgtggggc
     2521 cgtgcccagg gtacggggca tccaggcatc acgatgttca accgcggcgg cgggccgatc
     2581 accttcgatc cgctcaatcg cctggaccgg cagatgaacg cccacctgtt cctgttcggc
     2641 ccgacgggtt ccggcaaatc ggccacgctc aacaatctgc tgaaccaggt cacggccatt
     2701 tatcggccgc gcctcttcat cgtggaagcc ggcaatagct tcggcttgtt cagcgacttc
     2761 gccaggcgcc tgggcctgac cgtgaatcgg gtcaagctgg cccccggatc gggcatcagc
     2821 ctagcgccgt tcgccgacgc gcgccggctg atcgaaacgc ccagcgatgt gcaaacgctc
     2881 gatgccgatg cgctggacga agacctgcca cccgatgcct cagccatgga ggcggacgaa
     2941 cagcgcgacg tgctgggcga actggagatc acggcgcggc tgatgatcac tggcggcgag
     3001 gacaaggagg aagccaggat gacgcgcgcc gaccgctcgc tgatccgcca gtgcatcctc
     3061 gacgccgccg agcactgcgt ggccgagaag cgcacggtgc tcacgcgcga cgtgcgcaat
     3121 gcgctgcgca cccgcggcca ggacccgacg ttgcccgaga tgcggcgcgt gcggctgctg
     3181 gagatggcgg acgcgatgga catgttctgc caaggcacgg acggcgagat gttcgaccgc
     3241 gacggcacgc cgtggcccga ggccgacatc acgctggtgg atctcgcgac ctatgcccgt
     3301 gagggctaca acgcgcagct ctcgatcgcg tacatcagcc tcatcagcac ggtgaacaac
     3361 atcgcggagc gtgaccagta tctcggccgc ccgatcatca acgtgaccga cgaaggccac
     3421 atcatcacga agaacccgct gctcgcgccg tatgtcgtga aaattacaaa aatgtggcgc
     3481 aagttgggcg cctggttctg gctggcgacg caaaacatcg acgatctgcc gcgcgccgca
     3541 gagcccatgc tcaacatgat cgagtggtgg atctgcctgt cgatgccgcc ggacgaggtg
     3601 gagaagatcg cacgattccg cgaactctcg ccggcgcaga aggcgctgat gctttcggca
     3661 cgcaaggaag cgggcaagtt caccgagggc gtcatcctct ccaagagcat gg
//
LOCUS       DAOMVO010000247         3681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7324_length_3681_cov_17.164920, whole genome shotgun sequence.
ACCESSION   DAOMVO010000247 DAOMVO010000000
VERSION     DAOMVO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7324_length_3681_cov_17.164920"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKV30_10765"
     CDS             complement(<1..803)
                     /locus_tag="PKV30_10765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRL30726.1"
                     /translation="MAMLQLVLDLFGVAPAAPAFEPKAPPALEEQAPAAPQLIADEPA
                     VALGDALMPAHFAHPRANRAIDFAHARVHYEFQRGQRRTIGFSVGPDGLAVRAPRWTP
                     LHEVEAALREKERWIVAKLGEARERHARIESNRIDWKEGATLPFLGQPVTLVLDPRQQ
                     HGRGGAVLAEGDGAGVLHIGLPHTATPEQLRDVAQAWLMRQARRVFIARLDHFAPQLD
                     VRWQKLSLSSAGTRWGSASADGSIRLNWRLIHFREPIIDYVVVHELAHLR"
     gene            complement(810..1586)
                     /locus_tag="PKV30_10770"
     CDS             complement(810..1586)
                     /locus_tag="PKV30_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL30727.1"
                     /translation="MNLIRSILHMLWMIVTVIPWATAVLIASPFVSSTRLYWMCARWL
                     KLAVDSGTVIMGIQNRVTGMENLPLGAQDGAVLLVKHQSAWETFTMPAIMPHPLAYVF
                     KKELLYVPFFGWAMGRLDMIHIDRSQRAAAFNKVVQQGKRLMDQGVWVIMFPEGTRIP
                     RGEKGKYRSGGTRLAIECGAPVIPIAVTSAKCWPRKAFVKKPGIVDISIGPAIPSVGR
                     KPDELMREVEAWIEAEMVRLDPEAYPSGQPRLSRLKPGGD"
     gene            complement(1649..2275)
                     /gene="gmhB"
                     /locus_tag="PKV30_10775"
     CDS             complement(1649..2275)
                     /gene="gmhB"
                     /locus_tag="PKV30_10775"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HRL30728.1"
                     /translation="MKLVIVDRDGTLCVEREGYLQTPDDWAPLPGALDAIARLNHAGY
                     QVVIAANLPGLGRGLFDMAALNAIHAKMNKQVAAAGGRIEAVFFCPHTPDEGCNCRKP
                     LPGLFQQIGERYKLDLTQVHAVGDSERDVQAAVAAGCVPHLVLTGKAPPAQTDPLPPP
                     FPVGTRVHTDLAAFATTFIAEMQALDAARAETEAKARADKAPPPAAVH"
     gene            complement(2285..2899)
                     /locus_tag="PKV30_10780"
     CDS             complement(2285..2899)
                     /locus_tag="PKV30_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30729.1"
                     /translation="MSPAPETTAAPVAWRGLAWAALPPLLVLALRALMPGEVDAGALQ
                     RLETATLVSDPAEAFWMAARPLALGLLVLAVAAGACLFAVRRWGWARLRPALLGLWAA
                     MWLAMGVGLTASDLNRAQRQPLPDQSVKVLLAREILPGKRTGPGGTEVYFEQPGEAEP
                     MRLFAQGQPPAAFVPGSTALLHAEAGRWWGRWGRLTSRLAPASR"
     gene            complement(2902..>3681)
                     /locus_tag="PKV30_10785"
     CDS             complement(2902..>3681)
                     /locus_tag="PKV30_10785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRL30730.1"
                     /translation="YKPRFAGDELPRGQVGTVVALADKLETLVGMFGIGNLPTGDKDP
                     FALRRHALGVIRMLVEKKLPLNLPDVVQAAAQSFGALLADAARSNEQLLAFIYERVST
                     YLRDQGYSAQEADAVIAARPMWGEMPKALDAVRAFAQLPEAPALAAANKRIGNILKKA
                     EAEGAVDAHVNAALLQEPAEQALHDALLKVVPQADAQFDAGDYTGSLRTLSALRTPVD
                     AFFDGVMVNADATDVRLNRLGLLKTLHQAMNRVADLSRLAG"
BASE COUNT          538 a         1272 c         1306 g          565 t
ORIGIN      
        1 cgcaggtggg ccagctcgtg caccaccacg tagtcgatga tgggttcacg aaagtggatc
       61 aagcgccagt tcaggcggat cgagccatcg gcgctggccg agccccagcg cgtgccggcg
      121 gacgacaggc tgagcttctg ccaacgcacg tccagctgcg gcgcaaagtg atcgagccgc
      181 gcgatgaaca cgcggcgcgc ctggcgcatc agccaggcct gcgccacgtc gcgcaactgc
      241 tcaggcgtgg cggtgtgtgg caggccgatg tgcagcacgc ccgcgccgtc gccctcggcc
      301 agcacggcgc cgccgcgacc gtgttgttgg cgcggatcga gcaccagcgt caccggctgg
      361 cccagaaagg gcagcgtggc gccttccttc cagtcgatgc ggttggattc gatgcgcgcg
      421 tgccgctcgc gcgcctcgcc cagcttggcg acgatccagc gctctttttc gcgcagcgcg
      481 gcttcgactt cgtgcagcgg cgtccagcgc ggcgcgcgca cggccaggcc gtcggggccg
      541 accgaaaagc cgatggtgcg ccgctggccc cgctgaaact cgtagtgcac gcgggcgtgt
      601 gcaaagtcga tggcgcggtt ggcgcgcggg tgcgcaaagt gtgcgggcat gagcgcatcg
      661 cccagcgcca ccgcaggttc gtccgctatc agttgaggag ctgctggcgc ttgttcctca
      721 agcgctggcg gcgcttttgg ctcaaatgct ggcgctgccg gcgcgacgcc gaacagatcc
      781 agcaccaact gcagcatcgc catgcgcggt caatcgcctc cgggcttcag gcgcgacagg
      841 cgcggctggc cgctggggta ggcctcgggg tccaggcgca ccatctcggc ttcgatccag
      901 gcctcgacct cgcgcatcag ctcgtcgggc ttgcggccga cgctggggat ggccgggccg
      961 atggagatgt cgacgatgcc cggcttcttc acgaaggcct tgcgcggcca gcacttggcg
     1021 ctggtgacgg cgatggggat caccggcgcg ccgcattcga tggccagccg cgtgccgccg
     1081 gatcggtact tgcctttttc gccgcgcggg atgcgcgtgc cctcggggaa catgatgacc
     1141 cacacgccct ggtccatcag ccgcttgccc tgctgcacca ccttgttgaa ggcggcggcg
     1201 cgctggctgc ggtcgatgtg gatcatgtcg agccgcccca tcgcccagcc gaagaagggg
     1261 acgtagagca gctccttctt gaacacgtag gccaacgggt gcggcatgat ggccggcatg
     1321 gtgaaggtct cccacgccga ctggtgcttg accagcagca ccgcgccatc ctgggcgccc
     1381 agaggcaggt tctccatgcc ggtgacgcgg ttctggatgc ccatgatcac cgtgccgctg
     1441 tccaccgcca gcttgagcca gcgcgcgcac atccagtaca gacgcgtgct gctgacaaag
     1501 ggcgaggcga tcagcaccgc cgtcgcccac gggatgaccg tgacgatcat ccacagcatg
     1561 tgcagtatcg agcggatcag gttcatggtc tttcaggccg ctggcgcttg tctggcaagc
     1621 gcggacagct gcatatttta tagcattgtc agtggacggc ggcgggcggc ggcgccttgt
     1681 cggcgcgggc cttggcctcg gtttcggcgc gcgccgcatc gagcgcctgc atctcggcaa
     1741 tgaaggtggt ggcaaacgcg gccaggtcgg tgtgcacgcg cgtgccgacg ggaaagggcg
     1801 gcggcagcgg gtcggtctgc gctggcggcg ccttgccggt cagcaccagg tgcggcacgc
     1861 aaccggcggc cacggcggcc tgcacgtcgc gctcgctgtc gcccaccgcg tgcacctgcg
     1921 tcaggtcgag cttgtagcgc tcgccgatct gctggaacag gccgggcaac ggcttgcggc
     1981 agttgcagcc ttcgtcgggc gtgtgggggc agaaaaacac cgcctcgatg cgcccgcctg
     2041 ccgcggcgac ctgcttgttc atcttggcgt ggatggcgtt gagcgctgcc atgtcgaaca
     2101 gcccgcgccc caggccgggc aggttggccg caatcaccac ctggtagccc gcgtggttga
     2161 gtcgcgcgat ggcgtccagc gcgccgggca gcggcgccca gtcgtcgggc gtttgcaggt
     2221 agccctcgcg ctcgacacac agggtgccgt cgcggtcgac gatgacgagt ttcatgaacg
     2281 cgaattatcg gcttgcgggc gcgagccgtg acgtgagccg gccccagcgg ccccaccagc
     2341 gcccggcttc ggcgtgcagc agcgccgtgc tgccgggcac gaaggccgcg ggcggctggc
     2401 cctgggcgaa caggcgcatc ggctcggcct cgcccggctg ctcgaaatac acctcggtgc
     2461 cgccgggccc ggtgcgcttg ccgggcagga tctcgcgcgc cagcagcacc ttcacgctct
     2521 ggtctggcag cggctggcgc tgggcgcggt tcaggtcgct ggcggtcagc ccgacgccca
     2581 tggccagcca catggcggcc cacagcccga gcagcgcggg ccgcagccgc gcccaacccc
     2641 agcgccgcac ggcaaaaagg cacgcgccgg cggccacggc cagcaccagc agcccgagcg
     2701 ccagcggccg cgcggccatc cagaaggctt cggccgggtc ggacaccagc gtggccgttt
     2761 ccaggcgctg caaggcgccg gcatcgacct cacccggcat cagcgcacgc agcgccagca
     2821 ccagcagcgg cggcagcgcg gcccacgcca ggccgcgcca ggcgacgggc gcggccgtgg
     2881 tttcgggcgc gggcgacacg ctcaacccgc cagccgcgac agatcggcca cgcggttcat
     2941 ggcctggtgc agcgttttca gcaagcccag ccggttcagg cgcacgtcgg tggcgtcggc
     3001 gttgaccatc acgccgtcga agaaggcatc gaccggcgtg cgcagcgcgc tcagcgtccg
     3061 cagcgagccg gtgtagtcgc cagcgtcgaa ctgcgcgtcg gcctgcggca cgaccttcaa
     3121 caacgcgtcg tgcagcgcct gctcggccgg ctcctgcagc agggcggcgt tgacatgcgc
     3181 atcgaccgcg ccttcggcct cggccttttt caggatgttg ccgatgcgct tgttggcggc
     3241 ggcgagggcg ggggcttcag gcagttgggc gaaggcgcgg acggcgtcga gcgccttggg
     3301 catttcgccc cacatcgggc gcgcggcgat cacggcgtcg gcttcttgcg cgctgtagcc
     3361 ttgatcgcgc aggtaggtgg acacgcgttc gtagatgaag gcgagcaact gttcgttgct
     3421 cctggcggcg tcggccagca gcgcgccaaa gctctgcgct gcggcctgca ccacgtcggg
     3481 caggttcagg ggcaacttct tctcgaccag catgcggatg acgcccaacg catggcgcct
     3541 cagcgcaaac ggatccttgt cgcccgtcgg caggttgccg atgccgaaca tgccgaccaa
     3601 cgtctccagc ttgtcggcca gcgccaccac ggtgccgacc tggccgcgcg gcagctcgtc
     3661 gccggcaaag cgcggcttgt a
//
LOCUS       DAOMVO010000248         3677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7337_length_3677_cov_15.877140, whole genome shotgun sequence.
ACCESSION   DAOMVO010000248 DAOMVO010000000
VERSION     DAOMVO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7337_length_3677_cov_15.877140"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="asd"
                     /locus_tag="PKV30_10790"
     CDS             complement(<1..683)
                     /gene="asd"
                     /locus_tag="PKV30_10790"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518691.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HRL30731.1"
                     /translation="MSDRLAVLPQYLLPKKLVTQLAGRFASAELDGVTQAAISRFVAH
                     YGVDMSEAAEPDITRYASFNDFFTRALRAGARPLADADYVCPVDGAISQFGAIERDQI
                     FQAKGHHYSTCALLAGDTALAAEFEHGQFATIYLSPKDYHRIHMPCAGRLQRMVYVPG
                     DLFSVNPTTARGVPGLFARNERVVCVFDTARGPFVLVLVGATIVGSMATVWHGVVNPP
                     RPGEIKRWD"
     gene            complement(728..1945)
                     /locus_tag="PKV30_10795"
     CDS             complement(728..1945)
                     /locus_tag="PKV30_10795"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HRL30732.1"
                     /translation="MTKPQHMLVVGGGIGGLAAALALSQLGIRVTLLEQAEHIGEIGA
                     GIQLAPNAFAALDALGVGENARRRAVFTDELVMMDAVDASVVGRFPVGAAFRQRFGNP
                     YAVIHRADIHTSILEGVQQSQLITFKTSARVEHIEQDGARVSAIDQHGQRHTADALIA
                     ADGVRSVARQLLVGDAPRVSGHVVYRAVVPVADMPADLRINAPVVWAGPNCHLVHYPL
                     RGSTEYNLVVTFHSRYQEEWSVTEGSKEEVLSYFQGIHARPRQLLNCPKSWRRWATAD
                     RNPIPHWTVGRVTLLGDAAHGMLQYLAQGACMAIEDAVALREAMKASELDVGSAFKLY
                     EESRIPRTARVVLSAREMGRLYHAQGVERLVRNQLWEGRTPERFYDALDWLYGWRAED
                     ALVTPRQADALTG"
     gene            complement(2149..2847)
                     /locus_tag="PKV30_10800"
     CDS             complement(2149..2847)
                     /locus_tag="PKV30_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRL30733.1"
                     /translation="MTQYVFPPGPQAAAAVRGSSQLYPVSRIFCVGRNYAAHAREMGF
                     DPDREPPFYFNKTPADLVMTGATVPYALGTQNLHYEMELVIAIGKPAFRVSVDKALDC
                     VWGYACGLDMTRRDLQNTAKQMGRPWDFGKDFENSAVMSPLVPAAEIGHPAQGRIQLS
                     VNGQIKQDSDIKELIWSVPEVIANLSEYYHLQPGDLIYTGTPEGVGAVQPGDVITGKI
                     DGVGEIALTIGQPE"
     gene            complement(2903..>3677)
                     /locus_tag="PKV30_10805"
     CDS             complement(2903..>3677)
                     /locus_tag="PKV30_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRL30734.1"
                     /translation="TPTIYVGLQLILPGEVAPAHRHTPTAVRFVIEGEGGYTTVNGEK
                     CPMHKGDLILTPALNWHEHGHEGSGPVIWMDALDLPMIYAMEASYAIEGPSQPVRPEA
                     DASQNKYRRAGLLPYASLGRAQRADYPQIRYPWVDVRQALAALATVTPKDEAVQLAYV
                     NPETGRPCLPTLGFSAMQLRAGQDLALRKRSASGVIHVIEGRGESEIDGVTLQWTEGD
                     TLTIPTHAQVRHRAMGNDAYLFHVDDAPMQRALGIYEDF"
BASE COUNT          581 a         1179 c         1272 g          645 t
ORIGIN      
        1 tagtcccagc gcttgatctc gcccgggcgc ggcgggttga cgacgccgtg ccaaacggtg
       61 gccatgctgc cgacgatggt ggcgcccacc agcaccagca cgaaggggcc gcgcgcggtg
      121 tcgaacacgc acaccacgcg ctcgttgcgc gcgaacaggc ccggcacgcc gcgcgccgtg
      181 gtcgggttga cgctgaacaa gtcgcccggc acatagacca tgcgctgcag gcggccggcg
      241 cagggcatgt ggatgcggtg gtagtccttc gggctcaggt agatggtggc gaactggccg
      301 tgctcaaatt cggcggccag cgccgtatcg ccggccagca gcgcgcacgt gctgtagtgg
      361 tgacccttgg cctgaaagat ctggtcgcgc tcgatggcgc cgaactggct gatggcgcca
      421 tcgaccggac acacgtagtc ggcatcagcc agcgggcgcg cgccagcgcg cagtgcgcgg
      481 gtgaagaagt cgttgaagct ggcgtagcgg gtgatgtcgg gctcggccgc ctcgctcatg
      541 tcaacgccgt agtgggcgac aaaacgactg atcgcggctt gcgtgacacc gtccagttcg
      601 gcgctggcaa agcggcccgc cagttgagtg acgagcttct tgggcagcag gtattgcggc
      661 agaacggcaa ggcgatcgga catgggctga ttctagagaa gcgctcctcg cgagggggag
      721 cggcgattca gccagtcagc gcatcagcct gccgtggcgt gaccaacgca tcctccgcgc
      781 gccagccgta cagccagtcc agcgcgtcgt aaaagcgctc cggcgtgcgg ccctcccaca
      841 gctggttgcg caccagacgc tccacgcctt gcgcgtggta caggcgcccc atctcgcgcg
      901 ccgacagcac gacgcgcgcg gtgcgcggga tgcggctttc ttcatacagc ttgaaggcgc
      961 tgcccacatc cagctcggac gccttcatcg cttcgcgcaa tgccaccgcg tcttcgatgg
     1021 ccatgcacgc gccttgcgcc aggtactgca gcatgccgtg cgcggcgtcg cccagcagcg
     1081 tgacgcgccc aaccgtccag tgcgggatcg ggttgcggtc ggccgtggcc cagcggcgcc
     1141 agcttttggg gcagttcagc aactggcgcg ggcgcgcatg gatgccctgg aagtagctca
     1201 acacttcttc cttgctgcct tcggtcacgc tccactcttc ctggtagcgg ctgtgaaagg
     1261 tgacgaccag gttgtattcg gtgctgccgc ggagcgggta atgcaccagg tggcaattgg
     1321 ggccggccca caccacgggg gcgttgatgc gcaggtcggc cggcatgtcg gccaccggca
     1381 cgacggcgcg gtagaccacg tgaccagaga cgcgcggcgc atcgcccacc agcagctggc
     1441 gcgccaccga gcgcacgccg tcggcggcga tgagggcatc ggcggtgtgg cgctggccgt
     1501 gctggtcgat ggcggaaacg cgcgcaccgt cctgctcgat gtgctcgacg cgcgccgaag
     1561 tcttgaaggt gatcagctgc gattgctgca cgccttccag aatcgaggtg tggatgtcgg
     1621 cgcggtgaat caccgcgtac gggttgccaa agcgctggcg gaacgccgcg ccgacgggaa
     1681 agcgcccgac cacgctggca tccaccgcgt ccatcatcac cagttcgtcg gtgaagacgg
     1741 cgcggcggcg cgcgttttcg cccacgccaa gcgcgtcgag cgcggcaaag gcgttgggcg
     1801 ccagttgaat gccggcgccg atttcgccga tgtgttcggc ctgctccagc agcgtgacgc
     1861 ggatgcccaa ctggctcagc gccagcgccg ccgccaaacc gccgatgccg ccgccgacca
     1921 ccagcatgtg ctgcggcttg gtcatgatgt ctcctgtgga tgctctttta ggtgtagctg
     1981 cttgcgcacg gtgggcaagc gctggagggc gatttcattc aaaaccaaac agccggacgc
     2041 agagggcgca gagatttcgc agaggacgca aaagtaaatc cgaaaaagag atttcttctg
     2101 cgtcctctgc gagtttttgc gtcctatgcg tccggctgtt cggctgagtt actccggctg
     2161 cccgatggtc aacgcgatct cgcccacgcc atcgatcttg ccagtgatca catcgcccgg
     2221 ctgcaccgcg cccacgcctt cgggcgtgcc ggtgtagatc aaatcgcccg gctgcaggtg
     2281 gtagtactcc gacaggttgg caatgacttc gggcacgctc cagatcaatt ccttgatgtc
     2341 gctgtcctgc ttgatctgcc cattcaccga cagctggatg cggccctgcg ccgggtggcc
     2401 gatctcggcg gcgggcacca gcgggctcat cacggcgctg ttctcaaagt ccttgccgaa
     2461 gtcccacggc cggcccatct gcttggcggt gttctgcagg tcgcgccgcg tcatgtccag
     2521 cccgcaggcg tagccccaca cgcagtccag cgctttgtcc acgctcacgc ggaaggccgg
     2581 cttgccgatg gcgatgacca gttccatctc gtagtgcagg ttctgggtgc ccagcgcgta
     2641 gggcacggtg gcgccggtca tcaccaggtc ggcgggcgtc ttgttgaagt agaagggcgg
     2701 ctcgcggtcg gggtcgaagc ccatctcgcg cgcgtgggcg gcgtagttgc ggcccacgca
     2761 gaagatgcgg ctgaccgggt acaactggct ggaaccgcgc accgcagcgg cggcttgcgg
     2821 gccgggcggg aagacgtatt gggtcatggt gtttactctc aaataaatag ctggtggcgc
     2881 tttcctggta agcgctggaa ggtcaaaagt cttcgtagat gccgagcgcg cgctgcatcg
     2941 gcgcgtcgtc cacgtggaac aggtaggcgt cgttccccat ggcgcggtgc ctgacctgcg
     3001 cgtgcgtcgg aatggtcagt gtgtcacctt ccgtccactg cagggtgacg ccgtcgatct
     3061 cagattcccc gcgcccttcg atcacatgga tcacgccgct ggccgagcgt ttgcgcagcg
     3121 ccaaatcttg gcccgcgcgc agctgcatgg ccgaaaagcc cagcgtgggc aggcaggggc
     3181 ggccggtttc ggggttgacg taggccagct gcaccgcctc gtccttgggc gtgacggtgg
     3241 cgagcgcggc cagcgcctgg cgcacgtcca cccaggggta gcggatctgc gggtagtcgg
     3301 cacgctgcgc gcggcccagc gacgcatagg gcagcaggcc cgcgcggcgg tatttgtttt
     3361 gcgaggcgtc agcttcaggg cgcaccggct ggctggggcc ttcgatggcg taggacgctt
     3421 ccatggcgta gatcatgggc aggtccagcg cgtccatcca gatcaccggg ccgctgcctt
     3481 cgtggccgtg ttcgtgccag ttcagcgccg gggtcaggat caaatcgccc ttgtgcatgg
     3541 ggcacttttc gccgttgacg gtggtgtagc cgccctcgcc ttcgatcaca aagcgcaccg
     3601 ccgtgggcgt gtggcggtgg gcgggcgcca cctcgcccgg caggatgagc tgcaggccga
     3661 cgtagatggt gggcgtg
//
LOCUS       DAOMVO010000249         3629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7461_length_3629_cov_11.297985, whole genome shotgun sequence.
ACCESSION   DAOMVO010000249 DAOMVO010000000
VERSION     DAOMVO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7461_length_3629_cov_11.297985"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /gene="smpB"
                     /locus_tag="PKV30_10810"
     CDS             complement(<1..409)
                     /gene="smpB"
                     /locus_tag="PKV30_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRL30735.1"
                     /translation="MATKKPKSTSPVIAENKKAAFNYFFEDRFEAGMVLEGWEVKALR
                     AGKAQLTDGYVVIRNGELFVIGAQINPLGTASTHVRPDAVRTKKLLLHAEEIERLIGK
                     VEQKGYTLVPINLHWKNGRVKCEIALAKGKATHD"
     gene            505..960
                     /locus_tag="PKV30_10815"
     CDS             505..960
                     /locus_tag="PKV30_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRL30736.1"
                     /translation="MKSVHKSVLIWYSAQEMFDLVVDVERYPEFLPWCSHGKVLERTE
                     HGMTAEVGIAFKGVKQTFTTRNEHVPGREVRLHLVNGPFSQLEGVWTFTPVGEGDQRA
                     CRIDLKMDYGFSNRLLATLVGPVFDKIAASFVDAFVQRAEQVYGGTAAA"
     gene            957..1295
                     /locus_tag="PKV30_10820"
     CDS             957..1295
                     /locus_tag="PKV30_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HRL30737.1"
                     /translation="MTAPIHITVAYSAAPREVQEQALTLPAGSTVRDALVAAGLLDTA
                     LALADQGGVGVWGRKAPLDRPLEERDRIELWRPLRVDPKLARRERFGQQGARSAGLFA
                     KRRPGGKAGY"
     gene            complement(1496..2032)
                     /locus_tag="PKV30_10825"
     CDS             complement(1496..2032)
                     /locus_tag="PKV30_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HRL30738.1"
                     /translation="MKFTRAVLFGLTFAAVTAAHAQYQWVDKDGRRVFSDRPPPADVA
                     PKNIVSQPRGSTAAVVRSAPAAPANEPAAGEASVAAKPAAPAASAAPGAGVDKALEEK
                     KKQAEAAEAAKKKAEEQKIAAAKADNCKRAMNSKSSLDSGMRMARMNDKGEREVLDDA
                     QRAAELKRVNAIIASDCK"
     gene            2206..>3629
                     /gene="guaB"
                     /locus_tag="PKV30_10830"
     CDS             2206..>3629
                     /gene="guaB"
                     /locus_tag="PKV30_10830"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRL30739.1"
                     /translation="MRLLGNALTFDDVLLVPAFSQVLPKDVNLSTQFTRNIRLNLPLV
                     SAAMDTVTEARLAIAIAQEGGIGIVHKNLTAQEQAAQVAKVKRYESGVLRDPVVITPE
                     HTVLQVMQLSDELGISGFPVIDGGKVLGIVTGRDLRFETRYDVKVREIMTPRERLITV
                     NEGASAAEAKALLNKHKLERLLIIDDQDRLKGLITVKDITKQTNFPNAARDADGRLRV
                     GAALGVGEGTEERVEALVKAGVDALVVDTAHGHSKGVIERVRWVKQNYPHVDVIGGNI
                     ATGAAAKALAEAGADGVKVGIGPGSICTTRIVAGVGVPQITAIDNVATALAGTGVPLI
                     ADGGVRYSGDIAKAIAAGASSVMMGGMFAGTEEAPGEIVLFQGRSYKSYRGMGSIGAM
                     QQGSADRYFQESSTGNPNADKLVPEGIEGRVPYKGSVVSILFQMAGGLRASMGYCGCA
                     TIEDMRSTAEFVQITAAGIRESHV"
BASE COUNT          602 a         1191 c         1221 g          615 t
ORIGIN      
        1 tgtcgtgcgt ggccttgccc ttggccaggg cgatctcgca cttcacgcgg ccgtttttcc
       61 agtgcaggtt gatcggcacc agcgtgtagc ccttttgctc aactttgccg atcaggcgct
      121 cgatttcctc ggcgtgcagc agcagcttct tggtgcgcac ggcgtcgggc cgcacatgcg
      181 tgctggcggt gcccagcggg ttgatctgcg ccccgatgac gaacaactcg ccattgcgga
      241 tcaccacgta gccgtcggtc agctgcgcct tgccggcgcg cagggccttg acttcccagc
      301 cttccagcac catgccggcc tcgaagcggt cttcgaagaa atagttgaac gcggctttct
      361 tgttctcggc gatgaccggg ctggtcgact tgggtttctt ggtggccatt gctgtgcaat
      421 atgggtgcgc tgctcgcgct ttgcaagcag gcgggcggcg gcagtcaagg cgcgcgccta
      481 aaatcggcgg caaacgcgca ttctatgaaa tccgtccaca agtccgtcct gatctggtac
      541 agcgcgcagg agatgttcga cctggtggtc gacgtcgagc gctaccccga attcctgccc
      601 tggtgcagcc acggcaaggt gctggagcgc accgaacacg gcatgacggc cgaagtcggc
      661 atcgccttca agggcgtcaa gcagaccttc accacgcgca acgaacacgt gccggggcgc
      721 gaggtgcggc tgcacctggt caacggcccg ttctcgcagc ttgaaggcgt gtggaccttc
      781 acgccggtgg gcgagggcga ccagcgcgcc tgccgcatcg atttgaagat ggactacggc
      841 ttttccaacc gcctgctggc cacgctggtc ggcccggtgt tcgacaagat tgccgccagc
      901 tttgtcgatg ccttcgtcca gcgcgccgag caggtgtacg gcggcacggc ggccgcgtga
      961 ccgcgcccat ccacatcacc gtcgcctatt cggccgcgcc gcgtgaggtg caagagcagg
     1021 ccttgacgct gcccgctggc agcacggtgc gcgatgcgtt ggtggcggcg ggtttgctgg
     1081 acaccgcgct ggcgctggca gaccaaggcg gcgtcggcgt ctggggccgc aaggcgccgc
     1141 tggatcggcc gctggaagag cgcgatcgca tcgaactgtg gcgccccctg cgcgtcgacc
     1201 ccaagctggc gcggcgcgag cgctttggcc agcagggcgc gcgctcggcc gggctgttcg
     1261 ccaagcggcg gccgggcggc aaggcggggt attgatcgcg gaaaaaagcg cgccgactgg
     1321 cgcgcttttc gatgacggca tgacttcgct ttcgcttcaa tgacgacatg acctgacgcc
     1381 ccgtcatggc gcgccagcgc cgtcatcaac ggcgccagcc gaggtcatgc gccacaggcg
     1441 cgtcatcaaa aaagatagct gctcgcgctt gtccagcaag cgccagagcc ctatttcact
     1501 tgcaatccga cgcgatgatg gcgttgacgc gcttcagctc ggccgcacgc tgggcgtcgt
     1561 cgagcacctc gcgctcgccc ttgtcgttca tgcgggccat gcgcatgccg gaatcgaggg
     1621 acgacttgga gttcatcgcg cgcttgcagt tgtcggcctt ggcggcggcg atcttctgct
     1681 cttcggcctt tttcttggcg gcctcggcgg cttcagcctg ctttttcttt tcttccagtg
     1741 ccttgtcgac accggccccg ggcgccgcgc tggccgcagg cgcggccggc ttggctgcca
     1801 ccgacgcctc acccgctgcc ggctcgttgg ccggcgcggc gggcgccgag cgcaccaccg
     1861 ccgcggtaga gccgcgcggc tgcgacacga tgttcttggg ggcaacgtcg gcaggtggcg
     1921 ggcggtcgct gaacacgcgg cggccgtcct tgtcgaccca ctgatactgg gcgtgcgctg
     1981 ccgtgacggc ggcaaaggtc aatccaaaga gcacggcgcg ggtgaatttc atggggctgc
     2041 agtgtagcgg cgcggggcac cagacaccgc ttgtaacgcc gcgcggtgcc ccgtggccga
     2101 cagccggcgg cgccagacgt gcgttttgca cgccaaaggg catttgcgca ccacgggtgg
     2161 gcgaaaaagc cgctgtttac aatccgtctt ttggagcttt cagccatgcg ccttctcggc
     2221 aacgcgctta ctttcgacga cgttctgctc gtccccgcgt tctcccaggt cctgcccaag
     2281 gacgtcaatc tttccaccca gttcacccgc aacatccgcc tgaacctgcc gttggtttcg
     2341 gccgcgatgg acaccgtgac cgaagcgcgc ctggccattg ccatcgcgca agagggtggc
     2401 atcggcatcg tgcacaagaa cctcaccgcg caagagcagg ccgcacaggt cgccaaagtc
     2461 aagcgctacg agagcggcgt gctgcgcgac ccggtggtca tcacgcccga acacaccgtg
     2521 ctgcaggtga tgcagctgag cgatgagctg ggcatcagcg gctttccagt gatcgacggc
     2581 ggcaaggtgc tcggcatcgt gaccgggcgc gatttgcgct ttgaaacgcg ctacgacgtg
     2641 aaggtgcgcg agatcatgac cccgcgcgag cgcctcatca ccgtcaacga aggtgccagc
     2701 gcggccgaag ccaaggcgct gctcaacaag cacaagctgg agcgcctgct gatcatcgac
     2761 gaccaggacc ggctgaaggg cctgatcacg gtgaaagaca tcaccaagca gaccaacttc
     2821 cccaacgccg cgcgcgacgc cgacggccgc ctgcgcgtgg gcgccgccct gggcgtgggc
     2881 gagggcaccg aagagcgcgt cgaggcgctg gtgaaagccg gcgtggatgc gctggtggtc
     2941 gataccgcgc acggccacag caagggcgtg attgagcgcg tgcgctgggt caagcagaac
     3001 tacccgcacg ttgacgtgat cggcggcaac atcgccaccg gcgcggcggc caaggcgctg
     3061 gccgaggcgg gcgccgacgg cgtaaaagtc ggcatcggcc ccggcagcat ctgcaccacc
     3121 cgcatcgtcg ccggcgtggg cgtgccgcag atcaccgcta tcgacaacgt ggccacggcg
     3181 ctggccggta ccggcgtgcc gctgatcgcc gacggcggcg tgcgctactc gggcgacatc
     3241 gccaaagcca tcgccgccgg cgccagcagc gtgatgatgg gcggcatgtt cgccggcacc
     3301 gaagaagcgc cgggcgaaat cgtcctcttc cagggccgca gctacaaaag ctaccgcggc
     3361 atgggcagca tcggtgccat gcagcaaggc agcgccgacc gctactttca ggagagcagc
     3421 accggcaacc ccaacgccga taagctggtg cccgaaggca tcgaaggccg cgtgccgtac
     3481 aagggcagcg tggtcagcat cctgtttcag atggccggcg gcttgcgcgc cagcatgggg
     3541 tattgcgggt gcgcgacgat tgaggatatg cggtcaacgg cggagtttgt gcagatcacc
     3601 gcagcgggga ttcgtgaatc gcacgtgca
//
LOCUS       DAOMVO010000250         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7503_length_3615_cov_14.326404, whole genome shotgun sequence.
ACCESSION   DAOMVO010000250 DAOMVO010000000
VERSION     DAOMVO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_3615_cov_14.326404"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..693)
                     /gene="nusB"
                     /locus_tag="PKV30_10835"
     CDS             complement(199..693)
                     /gene="nusB"
                     /locus_tag="PKV30_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRL30740.1"
                     /translation="MSTEKQATPAAAGTRKSSTKSPRSRAREFALQGLYQHLVGNSDL
                     DVIDSFTRELQGFAKADSVHYDALLHGCIELKDDLDALIVPLLDRELGQISPVEHGCM
                     WIGAYEFLKCPDVPWRVVLNECIELAKDFGGTDGHKYVNAVLNGMAPKLRPHEVEADR
                     ARRR"
     gene            complement(690..1154)
                     /gene="ribH"
                     /locus_tag="PKV30_10840"
     CDS             complement(690..1154)
                     /gene="ribH"
                     /locus_tag="PKV30_10840"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRL30741.1"
                     /translation="MLGADKGQEQALNGSRLRIGIVQARFNEGVTGALHAACLAELTA
                     LGVRPENITHLTVPGALEVPSTLQWLARRGKVDALVALGCIIRGETYHFELVANESGA
                     GVTRVALDSGIPIANAILTTENMAQAVVRQVDKGRDAARVAVEMALIKQQLA"
     gene            complement(1141..2298)
                     /gene="ribBA"
                     /locus_tag="PKV30_10845"
     CDS             complement(1141..2298)
                     /gene="ribBA"
                     /locus_tag="PKV30_10845"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRL30742.1"
                     /translation="MNAPALPPSAISPVEDIVAELRAGRMVILVDEEDRENEGDLVLA
                     ADHVTPEAINFMARFGRGLICLTLTRERCERLALPPMVARNGTKMGTAFTVSIEAAEG
                     VTTGISAADRARTVQAAVARDANAADLVQPGHIFPLQAVEGGVLMRAGHTEAGCDLAG
                     MAGCSPAAVICEIMKDDGTMARLPDLQQFAAQHGLKIGTIADLIEHRSQHESLVEKVA
                     TRPLRTAHGEFVAHAYLDKPSGELHLALVMGQWAPGDAVPVRVHEPLSVLDALEVERA
                     NHSWSLHAALAHIQQAGRGVAVLLNCGEDAAQLLAQFEGTARAAHAPERGRMDLRSYG
                     VGAQILRDLGVQRMQVMGAPRRLPSMAGGYGLEIVEYVDHNDQGNTANARG"
     gene            2517..3470
                     /locus_tag="PKV30_10850"
     CDS             2517..3470
                     /locus_tag="PKV30_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL30743.1"
                     /translation="MNLDHPVASSLLPVVLLIGIGFVAGRMKLIRGEAVRDLSNLVFL
                     VLVQALLFRSMATAHLERLDLTSVALYYIVAGAMFFALLFMRGLNSRSAVLALASIFS
                     NTLMIGVPLVQLAYGEAALVNLFTLISMHALVLLTLATIVLELLVAREEAAAGHAAPR
                     HIAATVARAVKNAVLHPVPLPIIAGFLYSLTGFGLAPVVERPLKLLGDAFGPVALVLV
                     GVTLAQTAIGRHLKGALVISFLKTVIHPLLMWAAGWALGLSGLPLKVMVVAAALPVGA
                     NVFLFSQRYHKEEDLVTASVAVSTVMAVGTISVVMGLLAFV"
BASE COUNT          496 a         1147 c         1267 g          705 t
ORIGIN      
        1 tcaggtagat catcggccgg tcggtgccgg cggcctcgcg cgcgatggcg gcggccgatt
       61 tggccatctc catcacatag aagggttcga cgcgctgcgc gcgggcggag gttctcatgg
      121 cgccatgata ctcctgattt aatagctgct cgcgcttgtc catcaagcgc tggcgctcaa
      181 aaaaccttca aaacgaggtc agcggcgcct ggcgcgatcg gcctcgacct cgtgcggccg
      241 cagcttgggc gccatgccgt tgagcaccgc gttgacgtac ttgtggccgt cggtgccgcc
      301 aaagtccttg gccagttcga tgcattcgtt cagcaccacg cgccagggca cgtcggggca
      361 cttcaggaac tcgtacgcgc cgatccacat gcagccgtgt tcaaccggcg agatctggcc
      421 cagctcgcgg tcgagcagcg gcacgatcag cgcgtcgagg tcgtccttca gctcgatgca
      481 gccgtgcagc agcgcgtcgt agtgaaccga atcggccttg gcaaagcctt gcagttcccg
      541 cgtgaaggag tcgatcacgt ccaagtcgct gttgccgacc aggtgctggt acaggccctg
      601 cagcgcgaat tcgcgcgcgc gcgagcgcgg tgacttggtg gacgacttgc gcgtgccggc
      661 ggcggccggt gtcgcttgtt tctcggtact catgccagct gttgtttgat caaggccatc
      721 tcgaccgcca cgcgcgcggc gtcgcggccc ttgtcgacct ggcgcaccac ggcctgcgcc
      781 atgttctcgg tggtgaggat ggcgttggcg atggggatgc cgctgtccag cgccacgcgc
      841 gtgacgccgg cgccggattc attggccacc agttcgaagt gatacgtctc gccgcgaatg
      901 atgcagccca gcgcgaccag tgcatcgact ttgccgcgcc gcgccagcca ttgcagcgtg
      961 ctcggcactt ccagcgcgcc gggcacggtg aggtgggtga tgttctcggg ccgcacgccc
     1021 agcgccgtca gctcggccag gcaggcggcg tgcagcgcgc ccgtcacgcc ttcgttgaag
     1081 cgtgcctgca cgatgccgat gcgcaaccgg ctgccattca gcgcttgttc ctgtcccttg
     1141 tcagccccga gcatttgccg tgtttccttg atcgttgtgg tcgacgtatt cgacgatttc
     1201 aagcccatag ccgcccgcca tgctgggcag ccggcgcggc gcgcccatga cctgcatacg
     1261 ctgcacgcct aaatcgcgca ggatttgcgc gccgacgccg tagctgcgca agtccatgcg
     1321 gccgcgctcg ggcgcgtggg cggcgcgcgc cgtgccctcg aactgcgcca gcagttgcgc
     1381 cgcgtcttcg ccgcagttga gcaacacggc cacgccgcgc ccggcttgct ggatgtgcgc
     1441 cagcgcggcg tgcaggctcc acgaatggtt ggcgcgttcg acctccagcg cgtcgagcac
     1501 cgacagcggc tcgtgcacgc gcaccggcac cgcgtcgccc ggcgcccact ggcccatgac
     1561 cagcgccagg tgcagctcgc cgctcggttt gtcgaggtag gcgtgggcca cgaattcgcc
     1621 gtgcgcggtg cgcagcgggc gggtggcgac tttttcgacc agcgattcgt gctggctgcg
     1681 gtgctcgatc aaatcggcga tggtgccgat tttcaagccg tgctgcgctg cgaactgctg
     1741 cagatcgggc aggcgcgcca tggtgccgtc gtccttcatg atctcgcaga tcacggcggc
     1801 ggggctgcag ccggccatgc cggccagatc gcaacccgcc tcggtatggc cggcgcgcat
     1861 cagcacgccg ccttcgacgg cctgcagcgg gaagatgtgg cccggctgca ccaggtcggc
     1921 ggcgttggca tcgcgcgcga ccgcggcctg cacggtgcgg gcgcggtcgg cggcgctgat
     1981 gccggtggtc acaccctcgg ccgcttcaat gctgacggta aacgccgtgc ccatcttggt
     2041 gccgttgcgc gccaccatgg gcggcagcgc caggcgctcg cagcgctcgc gcgtgagcgt
     2101 gaggcaaatc aggccacgcc caaagcgcgc catgaagttg atggcttcag gcgtcacgtg
     2161 gtcggcggcc agcaccaggt cgccctcgtt ctcgcggtct tcctcgtcga ccaggatgac
     2221 catgcggccg gcgcgcagtt cggccacgat gtcttcgacc ggcgagatgg ctgacggggg
     2281 gagggcgggg gcgttcatgg tgatggcaaa gagctggcgc ggtgggcccg cgcaccgcgc
     2341 caggtgcgcg gcagttgaaa tcaggcccgc attatccctg ctttaccccg cccgggccgc
     2401 gacgcgaggc gcgcagcaag gaagggttac tatcgaaatg tgagctgctc gcgcttgtcc
     2461 ctccagcgca aacgccttgt ttggcttata aacaccggct tgccgaccgt tttgccatga
     2521 accttgacca ccccgttgcc tcgtccttgt tgcccgtggt gctgctgatc ggcatcggct
     2581 ttgtggccgg gcgcatgaag ctgatccgcg gcgaggcggt gcgcgatctg tccaacctgg
     2641 tgtttctggt gctggtgcag gcgctgctgt ttcgctcgat ggccacggcg cacctggagc
     2701 ggctggacct gacctcggtc gcgctgtact acatcgtggc cggcgcgatg ttctttgcgc
     2761 tgttgttcat gcgcgggctg aacagccgct cggccgtgct ggcgctggct tccatcttca
     2821 gcaacacgct gatgatcggc gtgccgctgg tgcaactggc ctatggcgag gcggcgctgg
     2881 tgaacctgtt cacgctgatc tcgatgcacg cgctggtgct gctgacgctg gccaccatcg
     2941 tgctggaact gctggtggcg cgcgaagaag ccgccgcagg ccacgcggcg ccgcgccaca
     3001 tcgccgccac ggtggcgcgg gcggtgaaga acgccgtgct gcacccggtg ccgctgccga
     3061 tcatcgcggg ctttttgtat tcgctgacgg gttttgggtt ggccccggtg gtcgagcggc
     3121 cgctgaagct gctgggcgat gcattcggcc ccgtggcgct ggtgctggtg ggcgtgacgc
     3181 tggcgcagac ggcgattggg cggcacctga agggcgcgct ggtcatctcg ttcttgaaga
     3241 cggtgatcca cccgctgctg atgtgggcgg cgggctgggc gctgggcctg tccggcctgc
     3301 cgctgaaggt gatggtggtg gcggcggcgt tgccggtggg ggcgaacgtt ttcttgttct
     3361 cgcagcgcta tcacaaggag gaagacctgg tgacggcgag tgtggcggtg tcgacggtga
     3421 tggcggtggg gacgatttcg gtggtgatgg ggttgttggc ttttgtttga gttttttggg
     3481 ctgcggcgct tgtctggcgt gcgctagcag ctattgattt gatagttttt ttgggtgttg
     3541 ggcgcgctgt cggccgggat ttcgccccgg cgggcgacct actttctttg ctccgccaaa
     3601 gaaagtaggc aaaga
//
LOCUS       DAOMVO010000251         3612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7514_length_3612_cov_15.896542, whole genome shotgun sequence.
ACCESSION   DAOMVO010000251 DAOMVO010000000
VERSION     DAOMVO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_3612_cov_15.896542"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKV30_10855"
     CDS             complement(<1..314)
                     /locus_tag="PKV30_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30744.1"
                     /translation="MSHPSMIRGALACSLLGFGLNAAAQTPALFKDADLKLGEKLIAQ
                     HKCNQCHAQKWTDDGKAIYRPKGRVNTPALLQTMVERCSTQLNLSLFPEEVTAMAAVL
                     NR"
     gene            complement(409..885)
                     /locus_tag="PKV30_10860"
     CDS             complement(409..885)
                     /locus_tag="PKV30_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30745.1"
                     /translation="MSAAPAWSRRRSLHALGLLALGRPVWAAGKALPLSVSLRDELAV
                     ALKARQPLVVMVSLHRCPWCEAVRNNYLAPMHAQEGLPVVQVDMRSTAPSRTPVGAAT
                     THDALVRGWGVKVAPTLLFLGPGGAEVAERLVGGATDFYAASLDKRLAQARQALGG"
     gene            complement(882..1862)
                     /locus_tag="PKV30_10865"
     CDS             complement(882..1862)
                     /locus_tag="PKV30_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL30746.1"
                     /translation="MRRVIDRRQLIQRCACAALGGVAWARYGSAWGQGAPLPDMKPQQ
                     VAPGCWFVEGLSALGSPANQNFISNAGFVVTSAGVVVIDALGSPALAERLVAEIKKIT
                     PQKITHVVLTHYHADHVYGLQVFEQLGARILAHQAGREYLYADTARLRLEQSRKDLAP
                     WIDEQTHLAAATEWIAARRELTVGDTRLVIQPVGPAHTPEDLAVYLPDKKVLYAGDLV
                     FRSRIPFVGQADSRNWIKSLDKLLAFDAAVVVPGHGPASTEARKDMQLTRDYLAYLRK
                     TMGQAAKDMTPFDEAYKATDWSQFEHLPLFAAANRMNAYNTYLLMEHEPE"
     gene            1910..3019
                     /locus_tag="PKV30_10870"
     CDS             1910..3019
                     /locus_tag="PKV30_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRL30747.1"
                     /translation="MPLELASWVERHGTGAVLAVGGAVIGALFGFFAQRSRFCLRAAV
                     IEFWHGKFAEKLSVWLLAFASALIAVQALILAGALDVSGARQLANRGSLSGALVGGLL
                     FGMGMIMTRGCASRLLVLSANGNLRALLSGLVFAVTAQAALAGALAPLRTEISSWWTV
                     DGGAARSLLARVGAGHWDGLVFGALWLVVAVIFSLRSGNRPWMWIGGIGTGLAVALAW
                     WFSYAVSQASFDPVQIQGLTFSGPSAEWLMRVLASPAPPFGFDAGMLPGVFIGSFLGA
                     LIGREWKVEGFKDGASMARYIVGAMLMGFGSMLAGGCAVGAGMTGGAIFALTAWITLA
                     AMFVGGGLMDRWLAEPVLTAQPATQQAPVVLQVAP"
     gene            complement(3088..>3612)
                     /locus_tag="PKV30_10875"
     CDS             complement(3088..>3612)
                     /locus_tag="PKV30_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRL30748.1"
                     /translation="ARAKKIIGGSCFLCHGAEGESASEVFPRLAGQNAEYIAKQLENF
                     KSGKRKSSAMAPMVAELSPDDMAALGRFYGSRPPHKEAPKDAPLAAVGQYIYSQGNRF
                     SGVPACASCHGPDGAGSNALPRLAGQHAAYLDNQLKQFNKRERNNDNAVMHTVVEKMT
                     PLEMAAVSEYLSGK"
BASE COUNT          500 a         1201 c         1245 g          666 t
ORIGIN      
        1 tcgcggttga gcaccgccgc catcgccgtc acttcttcgg ggaacaggct caggttcagt
       61 tgcgtgctgc agcgctcgac catggtttgc agcagcgcgg gcgtgttgac gcgccctttc
      121 ggccggtaaa tggccttgcc gtcgtcggtc cacttctgcg cgtggcactg gttgcacttg
      181 tgctgggcga tcagcttttc gcccagcttc aggtcggcgt ccttgaacag cgctggcgtc
      241 tgcgccgccg cgttcaggcc gaatccgagc aaggaacaag ccagcgcgcc gcgaatcatc
      301 gatgggtgtg acatgctgct ctcctggttc acatcatgat gaacgctaca ttaagaatag
      361 cttctcgcgc ttgtcagaca agcgccagag cccgaaaaca cctcaaactc aaccgcccag
      421 cgcctgccgc gcctgcgcca gccgcttgtc gagcgaggcc gcgtaaaaat ccgtcgcccc
      481 gcccaccagc cgctccgcca cctcggcgcc gcccgggccc agaaacagca gcgtgggcgc
      541 caccttcacg ccccagccgc gcaccagcgc gtcgtgcgtg gtggcagcgc cgaccggcgt
      601 gcgcgatggc gcggtactgc gcatgtccac ctgcaccacg ggcagcccct cttgcgcgtg
      661 catgggcgcc aggtagttgt tgcgcaccgc ctcgcaccac gggcagcggt gcaggctgac
      721 catgacgacc agcggctggc gcgccttcag tgccacggcc agttcatcgc gcaaagatac
      781 cgacagcggc agcgccttgc ctgccgccca caccgggcgg cccagcgcca gcagccccag
      841 cgcgtgcagg ctgcggcggc gtgaccatgc gggcgcggcg ctcattcggg ttcgtgctcc
      901 atcagcaaat aggtgttgta ggcgttcatg cggttggcgg cggcaaacag cggcaggtgc
      961 tcgaattggc tccagtcggt ggccttgtag gcctcgtcga acggcgtcat gtccttggcc
     1021 gcttggccca tggtcttgcg caggtaggcc aggtagtcgc gcgtgagctg catatccttg
     1081 cgcgcctcgg tcgatgccgg gccgtggccg ggcacgacca cggcggcgtc aaacgccagc
     1141 agtttgtcga gcgatttgat ccagttgcgg ctgtcggcct gccccacaaa cgggatgcgg
     1201 ctgcgaaaca ccagatcgcc cgcgtacaac accttcttgt ccggcaggta caccgccagg
     1261 tcttcgggcg tgtgtgcggg gccgacgggc tggatcacca ggcgcgtgtc gcccaccgtg
     1321 agctcgcggc gcgcggctat ccattcggtg gcggcggcca ggtgggtctg ctcgtcgatc
     1381 cagggcgcca gatccttgcg cgattgctcc agccgcaggc gcgcggtgtc ggcgtacagg
     1441 tattcgcgcc cggcctggtg cgcgaggatg cgcgcgccca gctgctcgaa cacctgcagg
     1501 ccgtacacgt ggtcggcgtg gtagtgggtc aggacgacgt gcgtgatctt ttgcggcgtg
     1561 atcttcttga tctcggccac cagccgctcg gccagcgcgg gcgagcccag cgcgtcgatc
     1621 accaccacgc cggccgaggt gacgacgaag cccgcgttgg agatgaagtt ctggttggcc
     1681 ggcgagccca gcgccgacag cccctcgaca aaccagcagc cgggcgccac ctgttgcggc
     1741 ttcatgtcgg gcagcggcgc gccctgcccc caggcgctgc cgtagcgcgc ccacgccacg
     1801 ccgcccaagg ccgcgcaggc gcagcgctga ataagttgac gtcgatcaat cacacgccgc
     1861 atgatgcgcc tttagtatct atcagttatt gctgatttaa ttattgaaca tgcccctcga
     1921 actggcaagc tgggtggaac gccatggcac cggcgccgtg ctggccgtgg gcggcgccgt
     1981 catcggcgcg ctgttcggtt tttttgccca gcgctcgcgc ttttgcctgc gcgcggcggt
     2041 gatcgagttc tggcacggca agttcgccga aaagctctct gtgtggctgc tggcctttgc
     2101 cagcgcgctg atcgcggtgc aggccctgat cctggccggc gcgctggacg tgagcggcgc
     2161 gcgccagctg gccaaccgcg gcagcctgtc gggcgcgctg gtgggcggcc tgctgtttgg
     2221 catgggcatg atcatgacgc gcggctgcgc cagccgcctg ctggtgcttt cggccaacgg
     2281 caatttgcgc gcgctgctgt cggggctggt gtttgccgtc accgcgcaag ccgcgctggc
     2341 gggcgcgctg gcaccgctgc gcaccgaaat ctccagctgg tggacggtcg atggcggcgc
     2401 cgcgcgcagc ctgctggcgc gcgtcggcgc gggccattgg gacggcctgg tgtttggcgc
     2461 gctgtggctg gtggtggcgg tgatcttctc gctgcgcagc ggcaaccggc cgtggatgtg
     2521 gatcggcggc atcggcaccg ggctggcggt ggcactggcg tggtggttca gctatgccgt
     2581 atcgcaggcc agcttcgacc cggtgcagat ccagggcctg acgttcagcg gcccctcggc
     2641 cgaatggctg atgcgcgtgc tggccagccc ggcgccgccg ttcggctttg acgccggcat
     2701 gctgccgggc gtgttcatcg gctcgtttct gggcgcgctc atcgggcgcg agtggaaggt
     2761 cgaaggcttc aaggacggcg ccagcatggc gcgctacatc gtcggcgcca tgctgatggg
     2821 ctttggcagc atgctggccg gcggctgcgc cgtcggcgcc ggcatgaccg gcggcgccat
     2881 ctttgcgctg accgcgtgga tcacgctggc cgcgatgttc gtgggcggtg ggctgatgga
     2941 ccgttggctg gccgagccgg tgttgacggc acagccagcc acgcagcagg cgccggtcgt
     3001 gctgcaggtg gcgccttgac cggagattga tgccaaacag gccgctggcg cacgtcaatc
     3061 aagcgcaagc agctatcaat tttgtagtta tttgccgctc aggtattccg acaccgccgc
     3121 catctccagc ggcgtcatct tctcgaccac ggtgtgcatc acggcgttgt cgttgttgcg
     3181 ttcgcgcttg ttgaactgct tgagctggtt gtccagatag gccgcgtgct ggccggccag
     3241 gcgcggcagc gcgttgctgc cggcgccatc cggcccgtgg caactggcgc aggccggcac
     3301 gccgctgaac ctgttgccct ggctgtagat gtactggccg acggcggcca gtggcgcgtc
     3361 tttcggcgct tccttgtgcg gcgggcggct gccgtaaaag cggccgagcg ccgccatgtc
     3421 gtcgggcgac agctcggcca ccatgggcgc catggcactg ctcttgcgct tgccgctctt
     3481 gaagttttcg agttgcttgg cgatgtactc ggcgttctgc ccagccagac gcggaaacac
     3541 ctcgctggcc gattcgccct cggcgccgtg gcacagaaag cacgagccgc cgatgatctt
     3601 cttggcccgc gc
//
LOCUS       DAOMVO010000252         3611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7517_length_3611_cov_16.924916, whole genome shotgun sequence.
ACCESSION   DAOMVO010000252 DAOMVO010000000
VERSION     DAOMVO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7517_length_3611_cov_16.924916"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKV30_10880"
     CDS             complement(<1..610)
                     /locus_tag="PKV30_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30749.1"
                     /translation="MNAGLLGMAGGPQRIVCLTEETTEWLYLLGEERRIAGISGYTVR
                     PRRARDEKPKVSAFTSAKLDKILALEPDLVLGFSDMQADIAAALIRAGLPVMVFNQRS
                     VAQIADMLAQLAALVGAAERGQALLLQFEERLALIQKAVQAQFALGKRRPRIYFEEWD
                     EPPISGIRWVSELIGIAGGEDVFPELAAQPLGKDRIIADPLEI"
     gene            complement(607..2004)
                     /locus_tag="PKV30_10885"
     CDS             complement(607..2004)
                     /locus_tag="PKV30_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HRL30750.1"
                     /translation="MADCPALLITAPASGQGKTTVTAALARMLARRGWRVRVFKCGPD
                     FLDPFWHQLASGAPVHNVDLWMTGEADIRARLHAAAGEADVLLVEGVMGLHDGTPSAA
                     DLARRFGLPVLAVVQAGAMAQTLGAVVHGLRHYKEDSNGRLPWAGVLANGVASAGHAA
                     LLQGAVDPPEGWLGHLPRSAAMQLPERHLGLVAAHELGQDAALARLDAAADLLAATSL
                     GALTLDDWRARWAVAFAAPAAGDADAVPPLLAGRTVAVARDAAFAFIYPANVATLQAL
                     GARVVFFSPLAGDALPECDALWLPGGYPELHAAALAARSDLRSALHRHAADGKPIWAE
                     CGGMMALFEELVTADGAAHAMWGLLPGRTTMQKKLAGLGMQAWAAAADAPPLRGHSFH
                     YSVCDTPLAPVGRTTPAPGSARREGEAVYVGGPRGAVRGSYFHAWLASSPRAAVRLFS
                     SGIGAGSACSKGDQP"
     gene            complement(2082..>3611)
                     /locus_tag="PKV30_10890"
     CDS             complement(2082..>3611)
                     /locus_tag="PKV30_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRL30751.1"
                     /translation="NRFTAVYIDGVRVDTQSGSGGAPWEAISLGQIERIEIVRGPAAA
                     VYGSDAIAGVIQIFTRKGEGPFTPYVGVGVGNRRTGKLEAGFSGASGAIDYSLGVERA
                     VSRGFNIKSDGNPDRDGYRQSAASGRLGWKVNDAHRVDLSGTASDMDAQYDGFAPGND
                     DHALSRLYTLGGTWSAQWTRNYSTRLSVSESRQRYETVPSPYLTKTTLRNYLLHNEWR
                     QGGHLVTAALERREDHLVNGPIDQGRHQNAVALGYGYSSGAHTLQANLRHDRDSEFGG
                     QTTGGLAYGYAFAPGWRVTGAVGTAFRVPTLYQRFSQYGQPGLEPEKSRNVELGLHWA
                     QAGSAFGVTVYRNRITNLINFGAPGPCSDSFGCYENTGRAVLQGVTVSGAHKLGGVNL
                     SGSFDYQDPRNDLTDKLLARRAQRTLKLNADTRVTTGALNWTLGAELQGVSHRWDNAA
                     NTNRLGGYGLVNLYASTTIAREWTLLARIDNLTDKDYQTARGYDTAGRTFYVGLKWTP
                     R"
BASE COUNT          513 a         1265 c         1296 g          537 t
ORIGIN      
        1 cgatctccag cgggtcggca atgatgcggt ccttgcccag cggctgggcc gcgagttcag
       61 gaaacacgtc ctcgccgccg gcgatgccga tcagctccga cacccagcgg atgccgctga
      121 tgggcggctc gtcccactcc tcgaagtaga tgcgcgggcg gcgcttgcca agggcgaatt
      181 gggcctgaac cgctttctga ataagcgcca gacgctcctc aaattgaagc aggagcgcct
      241 gcccgcgttc ggcggcgcca accagcgccg ccagctgcgc cagcatgtcg gcgatctgcg
      301 ccacgctgcg ctggttgaac accatcaccg gcaggccggc acggatcagc gcggcggcga
      361 tgtcggcctg catatcggaa aagcccagca ccaggtcggg ttcgagcgcg aggatcttgt
      421 cgagcttggc gctggtgaag gcgctcacct tcggcttctc gtcgcgcgcg cggcgcggcc
      481 gcacggtgta gccactgatg ccggcgatgc ggcgctcctc gcccagcagg tagagccact
      541 cggtcgtttc ctcggtcagg cagacgatgc gctgcgggcc gcccgccatc cccaacaggc
      601 ctgcattcat ggctgatcgc ccttgctgca tgccgacccg gcgccgatgc cgcttgaaaa
      661 aagccgcacc gccgcgcgcg ggctggaggc cagccaggcg tggaaatagc tgccgcgcac
      721 ggcgccgcgc gggccgccca cgtacacggc ttcgccctcg cgccgcgcgc tgccgggcgc
      781 gggcgtggtg cggcccacgg gcgccagcgg cgtgtcgcac accgaatagt gaaagctgtg
      841 gccgcgcagc ggcggcgcgt cggccgcagc cgcccaggcc tgcatgccca ggccggccag
      901 ctttttctgc atggtcgtgc gacccggcag caggccccac attgcgtgcg cggcaccgtc
      961 cgccgtgacc agctcttcga acagcgccat catgccgccg cattcggccc agatcggctt
     1021 gccgtcggcg gcgtgcctgt gcagggcgct ccgcaaatca gagcgcgcgg ccagcgcggc
     1081 ggcgtgcagc tcggggtagc cgccgggcag ccacagcgcg tcgcattcgg gcaacgcatc
     1141 gcccgccagc ggcgagaaga acaccacgcg cgcgcccagc gcctgcagcg tggccacgtt
     1201 ggcagggtag atgaaggcaa aagccgcatc gcgcgccacg gctacggtgc ggccggcgag
     1261 cagcggcggc acggcgtcgg catcgccggc ggcgggcgcg gcaaacgcca cggcccagcg
     1321 cgcgcgccag tcgtccagcg tcagcgcgcc cagcgacgtg gcggccagca ggtcggcggc
     1381 ggcgtccagc cgcgccagcg cggcatcctg gcccagttcg tgcgcggcca ccaggcccag
     1441 gtgccgctcg ggcagctgca tggcggcgct gcgcggcagg tggccgagcc agccctcggg
     1501 cggatcgacc gcaccctgca acagcgccgc gtgcccggcg ctggccacgc cgtttgccag
     1561 cacgccggcc cagggcaggc gcccgttgct atcctctttg tagtggcgaa ggccatgcac
     1621 gacggcgccc agcgtctgcg ccatggcgcc ggcctgcacc acggccagca ccggcaggcc
     1681 gaagcggcgc gccaggtcgg ccgcgctggg cgtgccgtcg tgcaggccca tcacgccttc
     1741 gaccaacagc acatcagcct cgccagccgc ggcgtgcagg cgcgcgcgga tgtcggcctc
     1801 gccggtcatc cacaggtcga cgttgtgcac cggcgcgccg ctggcaagct ggtgccagaa
     1861 cgggtccaga aagtccggcc cgcacttgaa cacccgcacg cgccagccgc gccgcgccag
     1921 catgcgcgcc agcgcggctg tgacggtggt cttgccctgg ccggaggccg gagcggtgat
     1981 caagagagcg gggcaatcgg ccatggtgcg cggctaaaga acacaacttg acgatggggc
     2041 atggtgtgcc gaaggggcac gccagccccg tgggcacgcg tttacctggg cgtccatttc
     2101 aggccgacgt agaaggtgcg gcccgccgtg tcgtaacccc gggccgtctg atagtccttg
     2161 tcggtcaggt tgtcgatgcg cgccagcagc gtccattcgc gcgcgatggt ggtgctggca
     2221 tacaggttga ccaggccata gccgcccagg cggttggtgt tggcggcgtt gtcccagcgg
     2281 tggctgacgc cttgcagctc ggcgcccagg gtccagttca gtgcgccagt ggtcacgcgc
     2341 gtgtcggcat tgagtttcag ggtgcgctgg gcgcggcgcg ccagcagctt gtcggtcagg
     2401 tcgttgcgcg gatcctggta gtcgaacgaa cccgacaggt tgacgccgcc cagcttgtgc
     2461 gcgcccgaca ccgtcacgcc ctgcagcacg gcgcggccgg tgttttcgta gcagccgaag
     2521 ctgtcgctgc acggtccggg cgcgccgaag ttgatgaggt tggtgatgcg gttgcggtag
     2581 acggtgacgc cgaaggcgct gccggcctgc gcccagtgca ggcccagctc gacgttgcgg
     2641 cttttctccg gctccaggcc gggctggccg tactgggaaa agcgctggta cagcgtcggc
     2701 acgcggaagg ccgtgccgac cgccccggtc acgcgccatc cgggcgcgaa ggcgtagccg
     2761 taggccagcc cgccggtggt ctggccgccg aattcgctgt cgcggtcgtg gcgcaggttg
     2821 gcctgcagcg tgtgcgcgcc gctgctgtag ccatagccca gggccacggc gttctggtgg
     2881 cggccctggt cgatggggcc gttgaccagg tgatcctcgc gccgctccag ggcggcggtg
     2941 accaggtggc caccctggcg ccactcgttg tgcagcaggt aattgcgcag cgtggtcttg
     3001 gtcaggtagg gcgagggcac agtttcgtag cgctggcgcg attcgctgac cgacagccgg
     3061 gtgctgtagt tgcgcgtcca ctgcgccgac caggtgccgc ccagcgtgta caggcgactc
     3121 agcgcatggt cgtcgttgcc cggcgcgaag ccgtcgtact gcgcgtccat atcgctggcg
     3181 gtgccgctca ggtcgacgcg gtgcgcgtcg ttgaccttcc agcccagccg gccgctggcg
     3241 gcgctctggc ggtagccgtc gcggtcgggg ttgccgtcgg acttgatgtt gaagccgcgg
     3301 ctcacggcgc gctcgacgcc gagcgaatag tcgatggcgc cgctggcgcc cgagaaacct
     3361 gcctccagct tgccggtgcg gcgattgccc acacccacgc cgacgtaggg cgtgaacggc
     3421 ccttcgccct tgcgggtgaa aatctggatc acgccggcaa tggcgtcgga gccgtacacc
     3481 gccgccgccg ggccgcgcac aatttcgatg cgctcgatct ggccgagcga aatcgcttcc
     3541 cacggcgcgc cgccactgcc cgattgcgta tcgacccgca cgccatcgat gtagacggcg
     3601 gtgaagcggt t
//
LOCUS       DAOMVO010000253         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7546_length_3600_cov_18.387306, whole genome shotgun sequence.
ACCESSION   DAOMVO010000253 DAOMVO010000000
VERSION     DAOMVO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7546_length_3600_cov_18.387306"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PKV30_10895"
     CDS             complement(<1..1822)
                     /locus_tag="PKV30_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HRL30752.1"
                     /translation="MSASKSSLGALTLGAIGVVYGDIGTSVLYTLKEVFGSGHVPFTH
                     DNVYGVLSIVFWTLTIIVSLKYVSLVLRADNHGEGGLVAMLALASQSVKDKAPALRKW
                     MLLIGIFGTCLFYGDGVITPAITVLSAVEGLDVVSPAFRKLVIPLTLLILFVLFWVQK
                     RGTAGIGKAFGPIMVLWFVVIAALGIYHIVGHPEILWALSPHHALRFIFAQPLITFII
                     LGAVVLCVTGGEALYADMGHFGKKPIRVAWFSIVMPCLTLNYFGQGALLLAEPEAVKN
                     PFFHMAPDWLTLPLVGLAAMAAVIASQALISGAFSVTKQVIQLGYLPRLQIRHTSTKD
                     TGQIYMPFVNWGLFVLIVLAVVLFRSSSNLAAAYGIAVTLDMTITTVLTFFVIRYGWG
                     YPLALCIAATGFFFAIDVTFFASNLLKLFEGGWFPILIGGAIFTLMTTWKDGRELLNQ
                     RLRDVSIDLQSFLESVFISPPARVAGTAIFLTAQADIVPNALMHNLKHNKVLHAQNLF
                     VTVRNHEVPWIGMNKRLEIEPLGHDCWQIAVNYGFKNDINLPKALGLLTGRGIDLNPM
                     TTSYFLSRDIVTPTIGAGMAMWREKLFAQMHHSATAAAEFL"
     gene            complement(2033..3322)
                     /gene="gshA"
                     /locus_tag="PKV30_10900"
     CDS             complement(2033..3322)
                     /gene="gshA"
                     /locus_tag="PKV30_10900"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRL30753.1"
                     /translation="MVPHLITALSGPINELEQRILDATPVIERWFRLEWMEHTPPLYA
                     SVDIRNAGFKLAPVDTNLYPDGWHNLSPDTLPLAVQAAQAAIEKICPEARNLLIVPEN
                     GKHGSFYLASLARLQEVFRMAGLNVRFGSVDPAVKRSQTITLPDGEKMTLEPAQRVRY
                     RVGLKNFDPCTILLNNDLSAGAPGILEELHEQYLLPPLQAGWSVRRKSRHFRCYEEVS
                     KRFGKLLGIDPWLINPMFASCGEIDIHEPSGGECLRTNVDALLVKIRRKYKEYGIHEK
                     PFVVVKADQGTHGMGIMTVRDVRDLDALGQKPRGKAAAPTVPKGEVIIQEGVLTNERI
                     HDAVAEPVVYMIDRYVVGGFYRVHAERAEDENLNAPGARFVPLAFDGGAQLPKLGVKA
                     GASAPNRFYMYGVIARLALVAASYELEATDPELQDAD"
     gene            complement(3428..>3600)
                     /locus_tag="PKV30_10905"
     CDS             complement(3428..>3600)
                     /locus_tag="PKV30_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HRL30754.1"
                     /translation="ASVMVNASTRFADGFEFGLGAEIGISTDKFHARGPVGIEGLTSL
                     KWVVLGEGEVRG"
BASE COUNT          638 a         1137 c         1219 g          606 t
ORIGIN      
        1 tgaggaactc ggccgcggcg gtggcgctgt ggtgcatctg ggcaaacagc ttctctcgcc
       61 acatcgccat gccggcgccg atcgtcggcg tgacgatgtc gcgcgaaagg aagtagctgg
      121 tcgtcatcgg gttcaggtcg atgccgcgcc ccgtgagcag gcccagcgcc ttgggcaggt
      181 tgatgtcgtt cttgaagccg tagttcaccg cgatctgcca gcagtcgtgg cccagcggtt
      241 cgatctcgag ccgcttgttc atcccgatcc acggcacctc gtggttgcgc acggtgacga
      301 acaggttttg cgcgtgcagc accttgttgt gcttgaggtt gtgcatcagc gcattgggca
      361 ctatgtcggc ctgcgccgtc aggaagatcg ccgtgcccgc cacgcgcgcg ggcgggctga
      421 tgaagaccga ttccagaaag gattggagat cgatcgagac atcgcgcagc ctctggttga
      481 gcagctcgcg cccgtccttc cacgtggtca tcagcgtgaa gatggcgccg ccgatcagga
      541 tcgggaacca gccgccctcg aacagcttga gcaggttgga ggcgaagaag gtgacatcga
      601 tggcgaagaa gaagcccgtg gccgcaatgc acagcgccag cggatagccc cagccgtagc
      661 ggatgacgaa gaaggtcagc acggtggtga tggtcatgtc cagcgtcacc gcgatgccgt
      721 aggccgccgc caggttggac gatgagcgaa acagcaccac cgccagcacg atcagcacga
      781 acaggcccca gttgacgaag ggcatgtaga tctggcccgt gtccttggtg ctggtgtggc
      841 ggatctgcag gcgcggcagg tagcccagct ggatcacctg cttggtgacg ctgaatgcgc
      901 ccgagatcag cgcctgcgac gcgatgacgg cggccatggc ggccaggccg accaaaggca
      961 gcgtgagcca gtcgggcgcc atgtggaaga acgggttctt caccgcctcg ggctcggcca
     1021 gcagcagcgc cccttggccg aagtagttga gtgtgaggca gggcatgacg atggagaacc
     1081 aggccacgcg gatcggcttc ttgccgaagt ggcccatgtc ggcgtacagc gcctcgccgc
     1141 cggtcacgca caacaccacg gcgcccagga tgatgaaggt gatgagcggc tgcgcgaaga
     1201 tgaagcgcag cgcgtggtgc gggctgagtg cccaaagaat ctcggggtgg ccgacgatgt
     1261 gataaatgcc cagcgccgcg atcaccacga accacagcac catgatcggc ccgaaggcct
     1321 tgccgatgcc cgccgtgccg cgcttttgca cccagaacag cacgaacagg atcagcagcg
     1381 tgagcgggat gacgagcttg cggaacgcgg gcgagaccac gtccagccct tcgaccgccg
     1441 acagcaccgt gatggccggc gtgatgacgc cgtcgccata gaacaggcag gtgccgaaga
     1501 tgccgatcag cagcatccac ttgcgcagcg cgggcgcctt gtccttcacg ctttgcgagg
     1561 ccagcgccag catggcgacc aggccgcctt cgccgtggtt gtcggcgcgc agcaccaggc
     1621 tgacgtactt gagcgagacg atgatggtga gcgtccagaa cacgatggac agcacgccgt
     1681 agacgttgtc gtgcgtgaac ggcacgtggc cggagccgaa cacctccttc agggtgtaga
     1741 gcacgctggt gccgatgtcg ccgtagacga cgccgatggc gcccagcgtc agggcgccca
     1801 gggacgattt ggaagcggac acgtcgcgct acaagcgccc ggctgaccgt gcgcgctcaa
     1861 gtggaaaggt ggctattgtg cgtcggccca gccgcacttg ggctgacggc gcgcgccgcc
     1921 tgtggtgggt cggcaacgtc cggatgaagg gcaaaaaagc acgccagcgc ttgtccatca
     1981 agcgccggca gctattgcag ttgtagcgat cagaaaaggc gcgaggcgcc actcaatccg
     2041 cgtcctgaag ctccggatcg gtcgcttcca gctcgtaact ggccgcgacg agcgccaacc
     2101 gcgcgatcac gccgtacatg tagaagcggt tgggcgcgct ggcaccggcc ttgacgccca
     2161 gcttgggcag ttgggcgccg ccgtcgaagg ccagcggcac gaagcgcgcg ccgggggcgt
     2221 tcaggttctc gtcctcggcg cgctcggcgt gcacgcggta gaagccgccc accacgtagc
     2281 ggtcgatcat gtagaccacc ggttcggcca cggcgtcgtg aatgcgctcg ttggtcagca
     2341 cgccttcctg gatgatgacc tcgcccttgg gcactgtagg cgccgccgcc ttgccacgcg
     2401 gcttttgccc cagcgcatcg aggtcgcgca cgtcgcgcac cgtcatgatg cccatgccgt
     2461 gcgtgccctg gtcggccttg acgacgacaa aaggcttttc gtgaatgccg tattccttgt
     2521 acttgcggcg gatcttgacc agcagcgcat cgacgttggt gcgcaggcac tcgccgccgc
     2581 tcggctcgtg gatgtcgatc tcgccgcagc tggcgaacat cgggttgatc agccacgggt
     2641 cgataccgag cagcttgcca aagcgcttgc tcacttcttc gtagcagcgg aagtggcggc
     2701 tcttgcgccg cacgctccag ccggcctgca gcggcggcag caggtattgc tcgtgcaact
     2761 cttccagaat gcccggcgcg ccggccgaca aatcgttgtt gagcaggatg gtgcaggggt
     2821 cgaagttctt caggccgacc cggtagcgca cgcgctgggc cggctccagc gtcatctttt
     2881 cgccgtcggg cagcgtgatg gtctggctgc gcttgacggc cgggtcgacc gagccaaagc
     2941 gcacgttgag ccccgccatg cgaaagactt cctgcagccg cgccaggctg gccaggtaaa
     3001 agctgccgtg cttgccgttc tccggcacga tgagcaggtt gcgcgcctcg gggcaaattt
     3061 tttcaatcgc cgcctgcgcc gcctgcacgg ccagcggcag cgtgtcgggc gacaggttgt
     3121 gccagccgtc ggggtacagg ttggtgtcga ccggtgccag cttgaagccg gcattgcgaa
     3181 tgtcgaccga ggcgtacagc ggcggcgtgt gctccatcca ctcgaggcga aaccagcgct
     3241 cgatgaccgg cgtggcatcg aggatgcgct gctccagctc gttgatcggg ccggagagcg
     3301 ctgtaatcag gtggggaacc atgcgtgtgg ggcgttgagc attgggcgtt gagcgattgt
     3361 ggcaccacgt tcgcgcttgc cgtcacgccc gctgcgaaac atcgaacgct gaaccgagcc
     3421 cacgcgctca gccgcgcact tcgccctcgc ccagcaccac ccacttcagc gaggtcaggc
     3481 cctcgatgcc gaccggcccg cgggcgtgga acttgtccgt gctgatgccg atttcggcgc
     3541 ccagcccgaa ctcgaagccg tcggcaaagc gcgtgctggc gttgaccatc acgctggccg
//
LOCUS       DAOMVO010000254         3597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7554_length_3597_cov_15.926030, whole genome shotgun sequence.
ACCESSION   DAOMVO010000254 DAOMVO010000000
VERSION     DAOMVO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7554_length_3597_cov_15.926030"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="PKV30_10910"
     CDS             <1..1805
                     /locus_tag="PKV30_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRL30755.1"
                     /translation="KYDGARAKGLPDGIEIHHRDKLDAVQREFREIPGTTVIIYDQTC
                     ATEKRRRRKRGTMVDPAVRVVINDLVCEGCGDCSVKSNCLSVEPLETEFGRKRAINQS
                     TCNKDYSCVNGFCPSFITVEGGQLKKKAKSQSKVSPFELGALPEPVQMQPDAGGAWGI
                     VVAGVGGTGVITIGQLLGMAAHMEGKGIVTQDAAGLAQKGGATWSHVLIGATQDDIRT
                     TRVGMAAADLIIGCDPIVTANKETVLRMREGRTHVALNSHGTPTAAFVKDASWQNPAE
                     QCAADVARAVGPSGLGMFDADAVASQLMGDTLYVNPMLLGYAWQKGWVPLARESLLRA
                     MELNNVQVENNKTAFEWGRQAAHDRARVERLLAGAGGAQVIEFKKRETLESIIARRVE
                     FLTGYQNAAYAQTYRDFVAKVQQEEAKLGKTALSEAVARYLFKLMAYKDEYEVARLHS
                     DPAFLKRIDDMFEGDYTLNYHLAPPMTADRNEKGELIKKKYGPSMLRNFKLLAKLKGL
                     RGGAFDVFGKTEERQTERALIQQYRASIEEVLRSLNADNHATAVEIARIPEQIKGYGH
                     VKERNLKPARERWNALMAQYRDPAATAAQAQRVA"
     gene            2050..2382
                     /gene="yajC"
                     /locus_tag="PKV30_10915"
     CDS             2050..2382
                     /gene="yajC"
                     /locus_tag="PKV30_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRL30756.1"
                     /translation="MFISNAYAQAAPAAAAGGDMSSSLMSMLPLVLMFVVLYFIMIRP
                     QMKKQKEHRAMIDALAKGDEVVTAGGMLGRVTSLSEGYIGVQVSNGVEVQMQRSAVVQ
                     VLPKGTIK"
     gene            2465..>3597
                     /locus_tag="PKV30_10920"
     CDS             2465..>3597
                     /locus_tag="PKV30_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRL30757.1"
                     /translation="MNRYPVWKYIIILITLVVGVLYTLPNFFGEAPAVQVSAAKATVR
                     VDGSTLSRVESVLQGASITPHRLTQEGTSVWARFDTPDTQLKAKDAIERALNPDAADP
                     SYIVALNLVPRSPAWLAAINAKPMYLGLDLRGGVHFMLQVDMRTAITKRLDTLANDIR
                     IALREKDVRHGGISREGNSIEIRARDAATLEAARRVITDTLPDLQVEQTQGAEPRLVA
                     TFKPAALQKVQSDAVKQNITTLHNRVNELGVAEPVIQQQGADRVVVQLPGVQDTAKAK
                     DILGRTATLEMRLVDESAEGRAAEAGGPVPFGSEKFLERDGRPVIVKRQVILTGDSLT
                     DAQPGFDSQTQQPKVDLTVDAKGGRVMRDVSRENLKKRMAIVL"
BASE COUNT          671 a         1206 c         1219 g          501 t
ORIGIN      
        1 agaagtacga cggcgcgcgc gccaagggcc tgccggatgg catcgagatc caccaccgcg
       61 acaagctgga tgcggtgcag cgcgagttcc gcgaaattcc gggcaccacg gtcatcatct
      121 acgaccagac ctgtgcgacc gaaaagcgcc gccgccgcaa gcgcggcacc atggtcgacc
      181 cggcggtgcg cgtggtcatc aacgacctgg tgtgtgaggg ctgtggcgac tgcagcgtga
      241 agagcaattg cctgagcgtg gagccgctgg agaccgaatt cggccgcaag cgcgccatca
      301 accagagcac ctgcaacaag gactattcct gcgtcaacgg tttctgcccc agcttcatca
      361 ccgtcgaagg cgggcagttg aagaagaagg ccaagagcca gtccaaagtg tcgcctttcg
      421 agctgggcgc gctgcccgag ccagtgcaga tgcagccgga cgcgggcggc gcctggggca
      481 tcgtggtggc gggcgtgggc ggcacgggcg tcatcaccat cggccagttg ctcggcatgg
      541 ccgcgcacat ggaaggcaag ggcatcgtca cgcaggacgc ggcgggcctg gcgcaaaagg
      601 gcggcgcgac ctggagccac gtgctgatcg gcgccacgca ggacgacatc cgcaccacgc
      661 gcgtgggcat ggcggcggcc gacctgatca tcggctgcga cccgatcgtc accgccaaca
      721 aggagaccgt gctgcgcatg cgcgaagggc gcacgcacgt ggcgctcaat tcgcacggca
      781 caccgacggc ggccttcgtc aaggacgcgt cgtggcagaa cccggccgag cagtgcgccg
      841 ccgacgtcgc gcgcgctgtg ggcccctcgg gcctgggcat gttcgatgcc gacgccgtgg
      901 ccagccagtt gatgggcgac acgctgtacg tcaacccgat gctgctgggc tacgcctggc
      961 aaaagggctg ggtgccgctg gcgcgcgaat cgctgctgcg cgccatggag ctgaacaacg
     1021 tgcaggtcga gaacaacaag accgccttcg agtggggccg ccaggccgcg cacgaccgcg
     1081 cgcgcgtcga gcggctgctg gcgggcgcag gcggcgcgca ggtgatcgag ttcaagaagc
     1141 gcgaaacgct cgaatcgatc atcgcgcggc gcgtggagtt cttgaccggc taccagaacg
     1201 ccgcgtatgc gcagacctac cgcgacttcg tcgccaaggt gcagcaggaa gaagccaagc
     1261 tgggcaagac agccctgagc gaagccgtgg cgcgctacct gttcaagctg atggcctaca
     1321 aggacgagta cgaggtggcg cgcctgcaca gcgacccggc gttcttgaag cggatcgacg
     1381 acatgttcga gggcgactac acgctcaact accaccttgc gccgcccatg acggccgacc
     1441 gcaacgagaa aggtgagttg atcaagaaga agtacggccc ctcgatgctg cgcaacttca
     1501 agctactggc caagttgaag ggcctgcgcg gcggcgcgtt cgacgtcttc ggcaagacgg
     1561 aagagcgcca gaccgagcgc gcgctgatcc agcagtaccg cgccagcatc gaggaagtgc
     1621 tgaggtcgct gaacgccgac aaccacgcca cggcggttga gattgcccgc attcccgagc
     1681 agatcaaggg ctacggccac gtgaaggagc gcaacctgaa gccggcgcgc gagcgctgga
     1741 acgcgctgat ggcgcaatac cgcgatccgg cggcgacggc ggcgcaggcg cagcgggtgg
     1801 cctgaaattc agggtcaaat aggccgctgg cgcttgatgg acaagcgcga gcagctccta
     1861 aaacaatagt gtttcgccat ggacttcgcc ccgaccttgc ggtcgggcgc gcggcggcgt
     1921 gcgcgccagg cgaagggcgg gcgcgcctta caatatgcgg ttgcctcgcg cgccgccccc
     1981 ggcgccccgg gccttgcgcg ctgcgcgctg cgcggcccat ttccctcaaa gcccttcagg
     2041 agtccgctgg tgttcatctc caacgcttac gcccaagccg cccccgccgc cgccgcgggt
     2101 ggcgacatgt cttcctcgct catgagcatg ctgccgctgg tgctgatgtt cgtggtgctg
     2161 tacttcatca tgatccgccc gcagatgaag aagcaaaaag aacaccgcgc gatgatcgac
     2221 gcgctggcca agggcgacga ggtggtgacc gccggcggca tgctgggccg cgtgacctcg
     2281 ctgtccgaag gctacatcgg cgtgcaggtg tccaacggcg tcgaggtgca gatgcagcgc
     2341 agcgccgtgg tgcaggtgct gcccaaggga accatcaagt aagcggccga ctcgcacccg
     2401 cgcggccttt gacgccgcgc accgtcccgc cgccgcccgg gccgctcctt tccccttccc
     2461 cgccatgaac cgctaccccg tctggaaata catcatcatc ctcatcacgc tggtggtggg
     2521 ggtgctctac accttgccga acttctttgg cgaggcgccg gcggtgcagg tgtccgcggc
     2581 caaggcgacg gtgcgggtcg acggcagcac gctgtcgcgc gtcgagagcg tgctgcaggg
     2641 cgccagcatc acgccgcacc gcctgacgca ggaaggcacg tcggtctggg cgcgtttcga
     2701 cacgcccgac acgcagctca aggccaagga cgccatcgag cgcgcgctca accccgacgc
     2761 ggccgacccc tcgtacatcg tggcgctgaa cctggtgccg cgctcgcccg cctggctggc
     2821 ggcgatcaac gccaagccga tgtacctggg tctggacttg cgcggcggcg tgcacttcat
     2881 gctgcaggtc gacatgcgca cggccatcac caagcgcctg gacacgctgg ccaacgacat
     2941 ccgcattgcg ctgcgcgaaa aggacgtgcg ccacggtggc atcagccgcg agggcaacag
     3001 catcgagatc cgcgcccgcg atgccgccac gctggaagcc gcgcgccgcg tcatcaccga
     3061 cacgctgccc gacctgcagg tcgagcagac gcaaggcgcc gagccgcgcc tggtcgccac
     3121 cttcaagccg gccgcgctgc aaaaagtgca gtccgacgcc gtcaaacaga acatcaccac
     3181 gctgcacaac cgcgtcaatg aactgggcgt ggccgagcca gtgatccagc agcagggcgc
     3241 cgaccgcgtg gtggtgcagt tgcctggcgt gcaggacacc gccaaggcga aagacatctt
     3301 gggccgaacc gccacgctgg agatgcgcct ggtcgatgaa agcgccgagg gccgcgccgc
     3361 tgaagccggt ggcccggtgc ccttcggctc ggagaaattc ctggagcgcg acggccgccc
     3421 ggtgatcgtc aagcgccagg tgatcctgac cggcgacagc ctgaccgacg cgcagcccgg
     3481 cttcgattcg cagacccagc agcccaaggt cgacctgacg gtggacgcca agggcggccg
     3541 cgtgatgcgc gatgtcagcc gcgagaacct gaagaagcgc atggccatcg tgctgtt
//
LOCUS       DAOMVO010000255         3597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7555_length_3597_cov_14.799831, whole genome shotgun sequence.
ACCESSION   DAOMVO010000255 DAOMVO010000000
VERSION     DAOMVO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7555_length_3597_cov_14.799831"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1465
                     /gene="rimO"
                     /locus_tag="PKV30_10925"
     CDS             56..1465
                     /gene="rimO"
                     /locus_tag="PKV30_10925"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRL30758.1"
                     /translation="MSNIPKVAFASLGCPKALTDSELILTQLSAEGYQTSKTFEGADL
                     VIVNTCGFIDEAVKESLDTIGDALNVNGKVIVTGCLGAKTIAGRAGQTSADGQNDSEN
                     LVKHLHPSVLAVTGPHATQEVMDAVHTHLPKPHDPFLDLVPGAFGEAGLKLTPRHYAY
                     LKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLNEARALFEGGVKELLVISQDTSAYGV
                     DLKYRTGFWDGRPVKTRLLELVQALGELAAPHGAWVRLHYVYPYPSVDEIIPLMAQGK
                     VLPYLDVPFQHSHPDVLRRMKRPASGEKNLERIARWREMCPELVIRSTFIAGFPGETE
                     AEFEHLLYFMREAGIDRAGCFAYSPVQGAAANDLPGMLPAELREERRARFMQVAEELS
                     TAKLQRRVGATMQVLVDSAPAMGRRGGVGRSYADAPEIDGTVQLLPPEKISKRLTVGE
                     FTRARVVGTQGHDLIALPV"
     gene            complement(1576..1660)
                     /locus_tag="PKV30_10930"
     tRNA            complement(1576..1660)
                     /locus_tag="PKV30_10930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1624..1626),aa:Leu,seq:cag)
     gene            1676..>3597
                     /gene="rnr"
                     /locus_tag="PKV30_10935"
     CDS             1676..>3597
                     /gene="rnr"
                     /locus_tag="PKV30_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRL30759.1"
                     /translation="MYIKNSDISGIALDEIEGTVQGHRDGHGFVVRDDGEPDIYLPPN
                     EMRAVLHRDRVKVRIVRQDRRGRPEGRVLEIAERPPHPIIGRLLHEGSIWLVAPEDKR
                     YGQDVLIPKTAIGAAKQGQVVVVELTEPPALYGQPVGRVKEVLGEIDDPGMEIEIAVR
                     KYGVPHEFSDAAMGEARALPDTVQPADKRGRVDLTDVPLCTIDGEDARDFDDAVYCEP
                     AVVSGRGKKPNGWRLLVAIADVSHYVKTGSPIDIDAYDRATSVYFPRRVIPMLPEKLS
                     NGLCSLNPDVERLCMVCDMMVSAKGEVLAYQFYPAVMFSHARFTYTEVAAILANTRGP
                     EAARRKDRVQDLLNLNDVFRALLKSRQTRGAVDFDTTETQIVCDEAGRIERIVPRVRN
                     DAHKLIEECMLAANVCSADFIQNSEHVGLYRVHEGPTPEKQDILRGYLKALGLGLSIS
                     ETPHPSEFQRIAEATKERPDAAQIHMMLLRSMSQAIYTPVNSGHFGLAYEAYTHFTSP
                     IRRYPDLLVHRVIKAVLAERQYQLPALPTPGEAHEKLKARLQKNSASRRGDAPPQLKR
                     QSPEMLAWEAAGLHCSANERRADEASRDVEAWLKCKYMKEHLGEEFGGTVSAATSFGL
                     FVTLDDLYVEGLVHIT"
BASE COUNT          678 a         1163 c         1169 g          587 t
ORIGIN      
        1 ctcatgcggg cccggcaggc cgggatgtgt caactattca gaggtgacgg ccgagatgtc
       61 caatattccc aaagtggcgt tcgcttcgct cggctgcccc aaggccctga ccgattcgga
      121 gctgatcctc acgcagctgt cggccgaggg ctaccagacc agcaagactt tcgaaggcgc
      181 tgacctcgtc atcgtcaaca cctgcggttt catcgatgaa gccgtcaagg aaagcctgga
      241 caccattggc gacgccctga acgtcaacgg caaggtcatc gtcacgggct gtcttggtgc
      301 aaaaacgata gctggtcgcg ctggccagac aagcgctgac ggccaaaatg attcagaaaa
      361 cctggtcaag cacctgcacc cttctgtgct ggccgtgacg gggccgcacg ccacgcaaga
      421 ggtgatggac gcggtgcaca cgcacttgcc caagccgcac gatccgtttc tcgacctggt
      481 gccgggcgcg ttcggcgagg cggggctgaa gctgacgccg cgccattacg cctacctgaa
      541 gatcagcgag ggctgcaatc accgctgcac cttctgcatc atcccgtcga tgcggggcga
      601 tctggtgagc cgccccattg gcgatgtgct gaatgaggcg cgcgcgctgt tcgaaggcgg
      661 cgtgaaggaa ctgctggtca tcagccagga cacgtcggcc tacggtgttg atttgaaata
      721 ccgcaccggc ttctgggatg ggcgtccggt gaagacgcgc ctgctggaat tggtgcaggc
      781 gctgggcgaa ctggctgcgc cgcatggcgc ctgggtgcgc ttgcattacg tttacccgta
      841 cccatctgtc gacgagatca tcccgctgat ggcccagggc aaggtgctgc cctacctgga
      901 tgtgcctttt cagcacagcc acccggatgt gctgcggcgc atgaagcgcc cggccagcgg
      961 cgagaaaaac ctggagcgca ttgcccgctg gcgcgagatg tgccccgagt tggtgattcg
     1021 atccaccttc atcgcgggct ttccgggcga gaccgaagcc gagttcgagc atctgctgta
     1081 tttcatgcgc gaggccggca tcgaccgcgc cggctgcttt gcctattcac cggtccaagg
     1141 cgcggccgcc aacgatttgc ccggcatgct gcccgctgaa ttgcgcgaag agcgccgcgc
     1201 gcgcttcatg caagtggccg aggagctgtc gaccgccaag ctgcagcgcc gcgttggcgc
     1261 caccatgcag gtgctggtcg attcggcgcc ggcgatgggc cggcgcggcg gggtggggcg
     1321 cagttacgcc gacgcgccag agatcgacgg cacggtgcaa ctgctgccgc ccgagaagat
     1381 cagcaagcgg ctgacggtgg gcgaattcac ccgcgcccgc gtcgtcggca cccagggcca
     1441 cgacctgatc gcgctgcccg tttgacggcc gcaaggccac ccaagcatga aaaaagccac
     1501 ccggaggtgg cttttgagta gggcgcgtct gctatcttca gcgcattgca gtggtagctg
     1561 catttttatt ctttttggtg cccaagagag gactcgaacc tccacgccgt taagcgctag
     1621 tacctgaaac tagtgcgtct accaattccg ccacctgggc atcaggaacc aaggattgta
     1681 tatcaaaaat tcagacattt ccggcatcgc gctggacgaa attgaaggca cggtgcaagg
     1741 ccaccgcgat ggccacggct tcgtggtgcg cgacgatggc gagcccgaca tttatctgcc
     1801 gcccaacgag atgcgcgcgg tgctgcaccg cgaccgtgtc aaggtgcgca tcgtgcggca
     1861 agaccggcgc ggccgccctg agggccgcgt gttggaaatc gccgagcgcc cgccgcaccc
     1921 catcatcggc cgcctgttgc atgaaggcag catctggctg gtggcaccgg aagacaagcg
     1981 ttacggccag gacgtgctga tccccaagac ggccattggc gcggccaagc agggccaggt
     2041 ggtggtggtc gagttgaccg agccgccggc gctgtacggc cagccggtcg gccgcgtgaa
     2101 agaggtgctg ggcgagatcg acgaccccgg catggagatc gagatcgccg tgcgcaagta
     2161 cggcgtgccg cacgaatttt cggacgccgc catgggcgag gcgcgcgcgc tgcccgacac
     2221 cgtgcagccg gccgacaagc gcggccgcgt cgacctgacc gacgtgccgc tgtgcaccat
     2281 cgacggcgaa gacgcgcgcg acttcgacga cgccgtctat tgcgaacccg ccgtcgtcag
     2341 cggccgcggc aagaagccca acggctggcg cttgctggtg gcgattgccg atgtcagcca
     2401 ctatgtcaag accggcagtc cgatcgatat cgacgcctac gaccgcgcca ccagcgtgta
     2461 ctttccgcgc cgcgtgattc cgatgctgcc cgagaagctg agcaacggcc tgtgctcgct
     2521 caaccccgat gttgaacgcc tgtgcatggt gtgcgacatg atggtgtcgg ccaagggcga
     2581 ggtgctggcc taccagttct acccggcggt gatgttcagc catgcgcgct tcacgtacac
     2641 cgaggtggcg gcgattttgg ccaacacgcg cggccccgag gcggcgcggc gcaaggaccg
     2701 ggtgcaggac ctgctgaacc tgaacgacgt gtttcgtgcg ttgctgaaaa gccgccagac
     2761 gcgcggcgcg gtcgatttcg acaccaccga aacgcaaatc gtctgcgacg aggccggccg
     2821 catcgaacgc atcgtgccgc gcgtgcgcaa cgacgcgcac aagctgatcg aagagtgcat
     2881 gctggccgcc aacgtgtgca gcgccgactt catccagaac agcgaacacg ttggcctgta
     2941 ccgcgtgcac gaaggcccta cgcccgaaaa gcaggacatc ctgcgcgggt acctgaaggc
     3001 gctcggcctg ggcttgtcga tcagcgagac gccgcacccg agcgagttcc agcgcattgc
     3061 cgaggccacc aaagagcgcc ccgacgccgc ccagatccac atgatgctgc tgcgctcgat
     3121 gagccaggcc atctacacgc ccgtcaacag cggccacttt gggctcgcgt acgaggcgta
     3181 cacgcacttc accagcccga tccggcgcta ccccgacctg ctggtgcacc gcgtcatcaa
     3241 ggccgtgctg gccgagcgcc aataccagtt gcccgcgctg ccgacgccgg gcgaagcgca
     3301 tgaaaagctc aaggcgcgct tgcaaaaaaa tagcgcctcg cgcaggggcg acgcgccgcc
     3361 gcagctgaaa cggcaatcgc ccgagatgct ggcctgggag gccgccggcc tgcactgcag
     3421 cgccaacgag cggcgcgccg acgaggccag ccgcgacgtc gaggcctggc tcaagtgcaa
     3481 gtacatgaag gagcacctgg gcgaagagtt tggcggcacc gtgagcgccg ccaccagctt
     3541 cggcctgttc gtgacgctgg acgatttgta cgtcgaaggc ctggtgcaca tcaccga
//
LOCUS       DAOMVO010000256         3563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7629_length_3563_cov_12.467218, whole genome shotgun sequence.
ACCESSION   DAOMVO010000256 DAOMVO010000000
VERSION     DAOMVO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7629_length_3563_cov_12.467218"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PKV30_10940"
     CDS             complement(<1..719)
                     /locus_tag="PKV30_10940"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HRL30760.1"
                     /translation="MKLNIVPARTGAQWVRAGVRTFFKQPLALAGLFFMFMAVISVLA
                     FVPMIGGVVALMAVPALTVGLMAAAREADEGRFPMPLTLFAGFRGSSQTRRGMVTLGV
                     LYAIGVVLVMGASALVDGGRFAQLYVQGGGITQELVMDPQFRMAMWLSTLLYLPVSLA
                     FWHAPALVHWHGVAPVKSLFFSLVAVLKNTRAFLLYGALWMVLSFAAGLVLLVLTALA
                     GSATIASVGLLPMALLIAAMF"
     gene            complement(716..1702)
                     /locus_tag="PKV30_10945"
     CDS             complement(716..1702)
                     /locus_tag="PKV30_10945"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRL30761.1"
                     /translation="MAVYTEVSEGEAGALLQSLGLGELTALRGIEGGIENTNYFASST
                     QGEWVLTLFERLTHAQLPFYLHLMKHLAQHGVPVPEPQAKAAGVRVRAGEDDILHTVC
                     GKPAAVVNRLRGKSELAPGPAHCAAVGDMLARAHLAARDYELRQPNLRGLAWWNETVP
                     VILPHLDESQAALIRAELAYQNHTGSSSAYAALPRGAVHADLFRDNVMFEGTPEQPVL
                     SGFFDFYFAGVDTWLFDLAVTLNDWAIDLPTGATHVARTQALLNAYQAVRPLADAERR
                     LLPAMLRAGALRFWVSRLWDFHLPREASMLKAHDPGHFERVLRQRIAAPLTA"
     gene            complement(1734..2078)
                     /locus_tag="PKV30_10950"
     CDS             complement(1734..2078)
                     /locus_tag="PKV30_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30762.1"
                     /translation="MRATLYFRAVLATVGLGLAAAVSAQPAPPASAPTVRDETVLDRR
                     TQRVEHIRTEDAGTRVDEVRSGGETKSIKVQPKAAVPAYEVRPADASGTGANSREAGP
                     GAAGARVWKLPF"
     gene            2137..>3563
                     /locus_tag="PKV30_10955"
     CDS             2137..>3563
                     /locus_tag="PKV30_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRL30763.1"
                     /translation="MTQAPEALAEPAAARPTLLLVDGSSYLYRAFHAMPDLRADPGDP
                     ASQPTGAIRGMINMLGALRKDYPGAQYAACIFDAPGKTFRDDLYPDYKAHRAPMPDDL
                     RSQIEPIHEAVRLLGWKVLAVPGVEADDVIGTLAQAGEAQGMDVIVSSGDKDLAQLVT
                     PHITIIDTMTGKRRDEAGVQAEFGVTASQMLDYQVLLGDAIDNVPGVDKVGPKTAAKW
                     LNEHGSLDAIVANVDQIKGAAGDNLRRALDWLPKGRELLKIRTDCDLAEYVAGLPALD
                     SVAVGAQQTGALADFYEKYGFKTLAKALQSGDAAKPARAKRASASDGPAIDLFADPPP
                     LSGEVTLDYEIILTWEAFDTWLAQINAAELTGIDTETTSLDEMRARIVGLSLSTAPGR
                     AAYIPLAHDGPDAPAQLPLDEVLARLKPWLEDASKKKLGQHVKYDQHVFANHGIRVAG
                     YAHDTMLQSYVLEVHRPHGLASLAER"
BASE COUNT          627 a         1237 c         1193 g          506 t
ORIGIN      
        1 aagaacatcg ccgcgatcag caaggccatc ggcagcaagc cgacggaggc gatcgtcgcg
       61 ctgccggcca gcgcggtcag caccagcagc acgaggccgg cggcaaacga cagcaccatc
      121 cacagggcgc catacagcag aaaggcgcgc gtgttcttca gcaccgcgac caggctgaag
      181 aacaggctct tgaccggcgc cacgccgtgc caatgcacca gcgcgggggc gtgccagaag
      241 gccagcgaca ccggcaggta gagcagggtg gacagccaca tggccatgcg gaactgcggg
      301 tccatcacca gttcttgcgt gatgccgccg ccttgcacgt aaagctgcgc gaagcggccg
      361 ccgtcgacca gcgccgacgc gcccatcacc agcaccacgc cgatggcgta cagcacgccc
      421 agcgtcacca tgccgcgcct ggtctggctg ctgccgcgaa agccggcgaa cagcgtcaac
      481 ggcatcggaa agcggccttc atcggcctcg cgcgcggccg ccatcaagcc gacagtgagc
      541 gcgggcacgg ccatcaacgc caccacgccg ccgatcatcg gcacgaacgc cagcaccgag
      601 atcacggcca tgaacatgaa gaacaggcca gccaatgcca gcggctgctt gaagaaagtg
      661 cgcacgccgg cgcgcaccca ctgtgcgccc gtgcgggcgg ggacgatgtt cagcttcatg
      721 ccgtcagcgg cgcggcgatg cgctggcgca gcacgcgttc gaaatggccg gggtcgtgcg
      781 ccttcaacat gctggcttcg cgtggcaggt gaaagtccca caggcgcgag acccagaagc
      841 gcagcgcccc ggcgcgcaac atggccggca gcaggcggcg ctcggcgtcg gcgagcggtc
      901 gcacagcctg gtaggcgttc agcagcgcct gcgtgcgcgc cacatgcgtg gcgcccgtgg
      961 gcaggtcgat ggcccagtcg ttcagcgtca ccgccagatc gaacaaccag gtgtcgacac
     1021 cggcaaagta gaagtcgaaa aaacccgaca gcaccggctg ctccggcgtg ccctcgaaca
     1081 tcacgttgtc gcggaacaga tcggcgtgca ccgcgccgcg cggcagggcg gcgtatgcgg
     1141 acgatgagcc ggtgtggttc tggtacgcca actcggcgcg aatcagcgcg gcctgcgatt
     1201 catccaagtg cggcaggatc accggcaccg tctcgttcca ccaggccagt ccgcgcaggt
     1261 tgggttggcg cagttcgtaa tcgcgcgccg ccagatgcgc gcgcgccagc atgtcgccca
     1321 cggcggcgca gtgcgcgggg ccgggcgcca gctcgctctt gccgcgcagt cggttcacca
     1381 cggccgcggg tttgccgcac acggtgtgca ggatgtcgtc ttcgcccgcg cgcacgcgca
     1441 cgccggccgc cttggcctgc ggctcgggca ctggcacacc gtgctgcgcc aggtgcttca
     1501 tgaggtgcag gtaaaagggc aactgtgcgt gcgtcaggcg ctcgaacagc gtcagcaccc
     1561 attcgccctg cgtgctgctg gcgaagtagt tggtgttctc gataccgcct tcgatgccgc
     1621 gcagcgccgt cagctcgccc aggcccagcg attgcagcag ggcgccggcc tcgccctctg
     1681 agacttcggt gtaaacagcc atctggcgct tgtctggcgt gcgcgaggcg ctcttaaaac
     1741 ggtagcttcc agaccctggc gccggccgcg ccgggaccgg cttcacggct gttggcaccg
     1801 gtgccgctgg cgtcggccgg ccgcacttca taggcgggca cggcggcctt gggctgcacc
     1861 ttgatgctct tggtctcgcc gccgctgcgc acttcatcca cgcgggtgcc ggcgtcttcg
     1921 gtgcgaatgt gctcgacgcg ctgggtgcgg cggtccagca cggtttcatc gcgaacggtc
     1981 ggggcgctgg cgggcggagc cggctgggcc gacacggcgg cggcaagccc caagccgacg
     2041 gtggccagaa cggcgcggaa atatagggtt gcacgcatgc cccgattcta gttggcgggt
     2101 gcgccacccc tgtaggacgg cgtgcacaat gccgccatga cccaagcccc cgaagcgctc
     2161 gcggaacccg cagccgcccg ccccacgctg ttgctggtcg atggcagcag ctacttgtac
     2221 cgcgcttttc acgccatgcc cgatttgcgc gccgacccgg gcgacccggc cagccagccc
     2281 accggcgcca ttcgcggcat gattaacatg ctgggcgcgc tgcgcaagga ctacccaggc
     2341 gcccaatacg ccgcctgcat cttcgacgcg cccggcaaga cctttcgcga cgacctgtac
     2401 cccgactaca aggcgcaccg cgcgcccatg cccgacgacc tccgcagcca gatcgagccg
     2461 atccacgaag cggtgcgcct gctgggctgg aaagtgctgg ccgtgcccgg cgtcgaggcc
     2521 gacgacgtga tcggcacgct ggcgcaggcg ggcgaggcgc aaggcatgga cgtgatcgtc
     2581 tccagcggcg acaaggatct ggcgcaactg gttacaccgc acatcaccat catcgacacc
     2641 atgaccggca agcgccgcga cgaggccggc gtgcaggccg agtttggcgt gacggccagc
     2701 cagatgctcg actaccaggt gctgctgggc gacgccatcg acaacgtgcc gggggtggac
     2761 aaggtcggcc ccaagacggc ggccaagtgg ttgaacgagc acggctcgct tgacgccatc
     2821 gtggcgaacg tcgatcagat caagggcgcg gcgggcgaca acctgcgccg tgcgctcgac
     2881 tggctgccca agggccgcga gctgctgaag atccgcaccg actgcgatct ggcggagtat
     2941 gtggcgggtc tgcctgctct ggattcggta gctgtcggcg cacagcagac gggtgctctg
     3001 gccgattttt atgagaaata cggcttcaag acgctggcca aggcgctgca gtcgggcgat
     3061 gcggccaagc cggcgcgggc caaacgcgcg tcggccagcg atgggcctgc cattgatctg
     3121 ttcgccgacc cgccgcccct gtcgggcgag gtcacacttg actacgaaat catactgacc
     3181 tgggaagcct tcgacacctg gctggcgcag atcaacgccg ccgagctgac cggcatcgac
     3241 accgaaacca cctcgctcga cgaaatgcgc gcgcgcatcg tcggcctgtc gctctccacc
     3301 gcgccggggc gcgcggccta cattccgctg gctcacgacg ggcccgatgc gccggcgcag
     3361 ttgcccttgg acgaggtgct ggcgcgcctc aagccgtggc tcgaagacgc cagcaaaaaa
     3421 aagctcggcc agcacgtcaa gtacgaccaa cacgtgttcg ccaaccacgg catccgtgtg
     3481 gccggctacg cgcacgacac catgctgcaa agctatgtgc tcgaagtgca tcgcccgcac
     3541 ggcctggcca gcttggccga gcg
//
LOCUS       DAOMVO010000257         3563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7630_length_3563_cov_11.027366, whole genome shotgun sequence.
ACCESSION   DAOMVO010000257 DAOMVO010000000
VERSION     DAOMVO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7630_length_3563_cov_11.027366"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKV30_10960"
     CDS             complement(<1..517)
                     /locus_tag="PKV30_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4130 domain-containing protein"
                     /protein_id="HRL30764.1"
                     /translation="MTPRFSAHAMPAPPVAGQPGGTDDAAAASNDVVASTAVFERFRQ
                     RARALLAAGVSPSAAVGTWVDANGHAAQGPGDLFAAASLPSAAITPPGGAPASANAAP
                     PVPRAFIALARRVVLHRAPGRFDALYALLWRLVHEPGLRHDPLDADLLRLRQMARAVQ
                     RDAYKMRAFVRF"
     gene            complement(525..1829)
                     /locus_tag="PKV30_10965"
     CDS             complement(525..1829)
                     /locus_tag="PKV30_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRL30765.1"
                     /translation="MELASKLAILADAAKYDASCASSGGTRRDSSRAGGGRGGIGSNE
                     GAGICHSFAPDGRCISLLKILLTNFCLYDCAYCINRSSSNVQRARFSVDEVVALTLDF
                     YRRNCIEGLFLSSGIIQSPDYTMEQLVRVARTLREVHDFRGYIHLKTIPDAAPDLLAQ
                     AGRWADRLSVNIELPTQAGLAQLAPEKDGGRIRRSMAALRLSIDDAQAQKREQQAQPI
                     RLLPGARPRRAAAPTFAAAGQSTQMIVGADGASDATILATSAQLYGAYRLKRVYYSAF
                     SPIPHASATLPAQAPPLLREHRLYQADWLLRFYGFAADEIAPQAGGMLSLDVDPKTAW
                     ALAHPERFPVDLDRAPRELLLRVPGLGVRAVTRLLMARRARRLRVADLTALRVPVRRV
                     LPFVVLVDHRPAPGAAQRLALAAVSRSKTALALAQPAQAALF"
     gene            1916..3562
                     /gene="guaA"
                     /locus_tag="PKV30_10970"
     CDS             1916..3562
                     /gene="guaA"
                     /locus_tag="PKV30_10970"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRL30766.1"
                     /translation="MQHQKILILDFGSQVTQLIARRVREAHVYCEVHPCDVTDDWVRE
                     YAADGTLKGVILSGSHASVYEVDDRAPDAVFALGVPVLGICYGMQTMAQQLGGKVEGG
                     HTREFGYAEVRARGHTALLKGIEDFHTPEGHGMLKVWMSHGDKVTALPPGFKLMASTP
                     SCPIAGMADEARRFYGVQFHPEVTHTVQGRALLERFVLDICGTRPDWVMQGHIEEAVQ
                     KIRDQVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGDMVMDMF
                     AGKLHAKVVRVDASDLFLRELAGVSDPEQKRKIIGRLFVDVFKAEAEKLKAAVATQNI
                     AAGARPSSAGATFLAQGTIYPDVIESGGAKSKKAVVIKSHHNVGGLPEQLGLKLLEPL
                     RDLFKDEVRELGVALGLPHDMVYRHPFPGPGLGVRILGEVKKEYADLLRRADAIFIEE
                     LRSTIEPNSGKSWYDLTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVQTSDFMTADW
                     AELPYALLKRASSRIINEVRGINRVTYDVSSKPPATIEWE"
BASE COUNT          601 a         1148 c         1276 g          538 t
ORIGIN      
        1 gaaaacgcac gaaggcgcgc atcttgtacg cgtcgcgctg cacggcgcgc gccatttggc
       61 gcaggcgcag caggtcggca tcgagcgggt cgtggcgcag gccgggctcg tgcaccagcc
      121 gccagagcag ggcgtacagc gcatcgaaac ggcccggcgc ccggtgcaac accacgcggc
      181 gggcaagggc gatgaaggcg cggggcacgg ggggcgcggc gttggcgctg gccggcgcgc
      241 cgccgggtgg ggtgatggcc gccgacggca aggaggctgc ggcaaacagg tcgccggggc
      301 cttgcgcggc gtggccgttt gcgtcgaccc acgtccctac agcggcgctg ggcgacacgc
      361 cggccgcgag cagggcgcgc gcccgctgcc gaaagcgctc gaacacggcg gtggacgcca
      421 ccacgtcatt gctggctgcg gcggcgtcat cggtgccgcc cggttggccc gcgacgggcg
      481 gcgctggcat ggcgtgggca ctgaagcggg gcgtcatctg ctattcaaaa aagagctgct
      541 tgcgctggtt gggcaagcgc cagggccgtt tttgaccttg aaaccgccgc cagggccagg
      601 cgctgcgccg cgccgggcgc gggccggtga tcgaccagca ccacgaaggg cagcacgcgc
      661 cgcacgggca cccgcagggc cgtcaaatcg gccacgcgca gccgccgcgc gcgccgcgcc
      721 atgagcaggc gcgtgacggc gcgcacgccg aggcccggca cgcgcagcag cagctcgcgc
      781 ggggcgcggt cgaggtcgac cggaaagcgc tcggggtgcg ccagcgccca cgcagtcttg
      841 ggatcgacgt cgagcgacag catgccgccg gcctgcggcg caatctcgtc ggcggcaaag
      901 ccatagaagc gcagcaacca gtcggcctgg tacagccggt gctcgcgcag cagcggcggc
      961 gcctgcgcgg gcagcgtggc cgaggcgtgc gggattgggc tgaaggccga gtagtacacg
     1021 cgcttgaggc ggtaggcgcc gtacagctgc gcgctggtgg cgaggatggt ggcgtcgctc
     1081 gcgccatcgg cgccgacgat catctgcgtg ctctggcccg cagcggcaaa ggtgggcgcg
     1141 gcggcgcggc gcgggcgggc gccgggcagc aggcggatcg gctgggcttg ctgctcgcgc
     1201 ttttgcgcct gggcgtcgtc aatcgacaaa cgcagcgccg ccatgctgcg gcgaatgcga
     1261 ccgccgtctt tttcgggcgc cagttgcgcc aagccggcct gtgtgggcaa ctcgatgttg
     1321 accgacaggc ggtcggccca gcgcccggcc tgcgccagca gatcaggcgc cgcatcggga
     1381 atcgtcttca ggtggatgta gccgcgaaag tcgtgcacct cgcgcagcgt gcgggccacg
     1441 cgcaccagtt gctccatggt gtagtcgggg ctctggatga tgccgctgga gagaaacagc
     1501 ccttcgatgc agttgcggcg gtagaagtcg agcgtgagcg ccaccacctc atcgaccgaa
     1561 aagcgcgcac gctgcacgtt gctgctgctg cggttgatgc agtacgcgca gtcgtacagg
     1621 cagaagttgg tgagcaggat tttcaacagc gaaatgcagc gaccatccgg cgcaaagctg
     1681 tggcagatgc ccgcgccttc attgctgccg atgccgccgc gcccgccgcc cgcgcgcgat
     1741 gaatcgcgcc gcgtgccgcc gctggaggcg cagctggcgt cgtacttggc ggcatcggcc
     1801 aaaatagcga gcttggaggc aagttccact gttcaaatgt acagttactg aaggcacctg
     1861 tccttggctt ttgacatacc ggacacatct tttttttgaa ctcggccgcc gcttcatgca
     1921 acaccagaaa atcctcatcc tcgactttgg ctcccaggtc acccaactca tcgcccgccg
     1981 cgtgcgcgag gcgcatgtgt attgcgaggt gcacccgtgc gacgtgacgg acgactgggt
     2041 gcgcgagtac gcggccgatg gcacgctgaa gggcgtgatc ctttccggca gccacgccag
     2101 cgtgtacgag gtggacgacc gcgcgcccga cgccgtgttt gccctgggcg tgcccgtgct
     2161 gggcatctgc tacggcatgc agaccatggc gcagcagttg ggcggcaagg tagagggcgg
     2221 ccacacgcgc gaatttggct acgccgaagt gcgtgcgcgc ggccacacgg cgctgctcaa
     2281 gggcatcgag gatttccaca cgcccgaagg ccacggcatg ctcaaggtgt ggatgagcca
     2341 cggcgacaag gtgaccgcgc tgccgccggg cttcaagctg atggcgtcga cgccaagctg
     2401 ccccatcgcc ggcatggccg acgaggcgcg gcgcttctat ggcgtgcagt ttcaccccga
     2461 ggtcacgcac acggtgcagg gcagggcgct gctggagcgc tttgtgctcg acatctgcgg
     2521 cacgcggccc gattgggtga tgcagggcca cattgaagaa gcggtgcaga aaatccgcga
     2581 tcaggtgggc gatgaggaag tgatcctcgg cctgtcgggc ggcgtcgatt catccgtggc
     2641 ggcggcgctg atccaccgcg cgattggcga tcaactcacc tgcgtgttcg tcgaccacgg
     2701 cctgctgcgc ctgaacgagg gcgacatggt gatggacatg tttgcgggca agctgcatgc
     2761 gaaggtggtg cgcgtggatg cgagcgatct gtttctgcgc gaactggcgg gcgtgagcga
     2821 cccggagcag aagcgcaaga tcatcggccg gctgttcgtc gacgtgttca aggccgaggc
     2881 ggaaaagctc aaggccgctg ttgctacgca aaacatagct gctggcgcac gtccatcaag
     2941 cgctggcgcc actttcttgg cccaaggcac gatctacccg gacgtcatcg aatccggcgg
     3001 tgccaagtcg aagaaggccg tcgtcatcaa aagccaccac aacgtcggcg gcctgcccga
     3061 gcagctgggc ctgaagctgc tggagccgct gcgcgacctg ttcaaggacg aggtgcgcga
     3121 gttgggcgtg gcgcttggcc tgccgcacga catggtgtac cgccacccct tcccgggtcc
     3181 gggcctgggc gtgcgcatcc tgggcgaggt gaagaaggaa tacgccgacc tgctgcgccg
     3241 cgccgacgcc atcttcatcg aagagctgcg ctcgaccatc gagccgaaca gtggcaagag
     3301 ctggtacgac ctgaccagcc aggctttcgc cgtcttcctg cccgtcaaaa gcgtcggcgt
     3361 gatgggcgac ggccgcactt acgattacgt cgtcgccctg cgcgccgtgc aaaccagcga
     3421 cttcatgacc gccgactggg ccgagctgcc ctacgcgctg ctcaagcgcg ccagcagccg
     3481 catcatcaac gaggtgcgcg gcatcaaccg cgtcacctac gacgtgtcga gcaagccgcc
     3541 ggcgacgatc gagtgggagt gaa
//
LOCUS       DAOMVO010000258         3515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7786_length_3515_cov_13.585260, whole genome shotgun sequence.
ACCESSION   DAOMVO010000258 DAOMVO010000000
VERSION     DAOMVO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7786_length_3515_cov_13.585260"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKV30_10975"
     CDS             <1..736
                     /locus_tag="PKV30_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL30767.1"
                     /translation="AWRLDLPALLAAITPRTKLLVVNSPNNPTGWTLSKDEQTAILTR
                     CRKTGTWILSDEVYERLYFRGDTASGAAPSFLDVARPDDRLVVVQSFSKAFLMTGWRL
                     GYLVMPPDMTPHVGKLLEFNTSCAPVFVQRAALVALQQTESITPRIVAHLKTCRDTLV
                     PLLQTVPGVQTSAAPGGMYAFLRVAGHDDSLQLAKRLVAEAGLGLAPGIAFAPEAEGW
                     LRWCFASKDVARLGQGVDRLRTWLAA"
     gene            746..1165
                     /gene="speD"
                     /locus_tag="PKV30_10980"
     CDS             746..1165
                     /gene="speD"
                     /locus_tag="PKV30_10980"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRL30768.1"
                     /translation="MRIPPDTYSATKPQPRVVARQSPGLHLIGDLYGCQGHAGLMTDA
                     PALEAFCKQAVLDAGLTIVGSLFHSFGAGQGVTGAVVLAESHLAIHTWPEDALVTLDV
                     YVCNYRGDSSARAEALFGALQQAFAPTDARVHRVGRR"
     gene            1288..1554
                     /gene="rpsO"
                     /locus_tag="PKV30_10985"
     CDS             1288..1554
                     /gene="rpsO"
                     /locus_tag="PKV30_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRL30769.1"
                     /translation="MISKENKAAVVKDNARAASDTGSPEVQVAILTARINELTPHFKQ
                     HAKDHHGRRGLLRMVSRRRKLLDYLKGKDADRYLALIQKLGLRK"
     gene            1774..>3515
                     /gene="pnp"
                     /locus_tag="PKV30_10990"
     CDS             1774..>3515
                     /gene="pnp"
                     /locus_tag="PKV30_10990"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRL30770.1"
                     /translation="MSLFNKVTKSFQWGDKTVVMETGEIARQATGAVLVDIDGTVILA
                     TVAASKSAKPGQDFFPLTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIR
                     PLFPDGFFNEVHVVIHTLSLNPEVDADIAAMIGVSAALSISGIPFNGPIGAARVGYVN
                     GEYVLNPGPTARKSSALDLVVAGTESAVLMVESEANQLTEDVMLGAVVFGHDQGKIAI
                     NAIHELVREAGKPLWADNGTWAPEVKDEAFIGKIGALAEPKLREAYQIRSKQARTQAL
                     REATAAVKQALTEEAVAFDPVKLDNLLFEIEARIVRGQILAGEPRIDGRDTRTVRPIE
                     IRNSVLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALAGEFEDRFLFHYNMPPFA
                     TGEAGRMGSTKRREVGHGRLAKRALIPLLPAKDEFPYTIRVVSEITESNGSSSMASVC
                     GGCLSMMDAGVPMKAHVAGIAMGLIKEDNRFAVLTDILGDEDHLGDMDFKVAGTTAGI
                     TALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKAEISSCAPKLYTMKI
                     NPEKIRDVIGKGGAT"
BASE COUNT          661 a         1171 c         1129 g          554 t
ORIGIN      
        1 cgcgtggcgg ctggatttgc cggcgttgct ggctgccatc acgccaagaa ctaagctgct
       61 ggtggtcaat tcgcccaaca acccgacggg ctggacgctc tcgaaagatg agcagacggc
      121 gattctgacg cgctgccgta agaccggcac ctggattctg tccgacgagg tgtacgagcg
      181 cctgtacttc cgcggcgaca ccgccagcgg cgccgcgccc agctttctgg acgtggcccg
      241 gcccgacgac cggctggtgg tggtgcagag cttttccaag gccttcctga tgaccggctg
      301 gcgcttaggg tatctggtga tgccgcccga catgacgccg cacgtgggca agctgctcga
      361 attcaatacc tcgtgcgcgc cggtgttcgt gcagcgcgcc gcgctggtgg cgctgcagca
      421 gaccgaatcc atcacccccc gcatcgtggc gcacctgaaa acctgccgcg acacgctggt
      481 gccgctgctg caaaccgtgc ccggcgtgca gaccagcgcc gcgccgggcg gcatgtacgc
      541 ctttttgcgc gtggctgggc atgacgattc gctgcaactg gccaagcgcc tggtcgccga
      601 agctggcctg ggcctggcgc ccggcattgc cttcgcgcct gaggcggaag gctggctgcg
      661 ttggtgcttt gcgtcgaaag acgtggcgcg gctggggcag ggcgtggatc ggctgcgcac
      721 ctggctcgcc gcttgacttg gggacatgcg catcccgccc gacacctatt ccgcgacgaa
      781 gccgcagccc cgcgtcgtcg cccgccagag ccccggtttg cacctgatcg gtgacctgta
      841 cggctgccaa ggccatgccg gcctgatgac cgacgctccc gcgctcgaag ccttttgcaa
      901 gcaggcggtg ctggatgcgg gcctgaccat cgtcggcagc ctgttccaca gctttggcgc
      961 ggggcagggc gtcacaggcg cggtggtgct ggccgaatcg catctggcga tccacacctg
     1021 gccggaagac gcgctggtga cgctggatgt atacgtctgc aactaccgcg gcgacagcag
     1081 cgcccgggcc gaagcgctgt ttggcgcgct gcagcaggcg tttgcgccga ccgacgcgcg
     1141 cgtgcaccgg gtagggcggc gttgaaccga cgcgccaagc atcgcctcgg gctataatcc
     1201 tcaggttttg caactcgcaa tacgcacgtg gcgtgcagtt ccggcgcgcc tcgcaagtca
     1261 aaccatttgg aacgaaggta acgacagatg atctccaaag agaacaaggc cgccgtggtc
     1321 aaggacaacg cgcgcgccgc cagcgacacg ggcagccccg aagtgcaagt ggccatcctc
     1381 acggcccgca tcaacgaact gaccccccac ttcaagcagc acgccaagga ccaccacggt
     1441 cgccgcggtc tgctgcgcat ggtcagccgc cgccgcaagc tgctggacta cctgaagggc
     1501 aaggacgccg accgctacct ggccctgatc cagaagctgg gtctgcgcaa gtaagcgcat
     1561 gaccacgaaa aacgcctgat gcctcaagtg gctcaggcgt ttttgtcttt tttcaacccg
     1621 aaggcccgtt cgtttagaag ctgaagctgt gtcattccaa taaccgctcc cgtgcgaccg
     1681 gggcgattcc tggaatggca tcgtgttctg aatgaaatgt ggctccagcg cacgacgggt
     1741 gtgcgcaagc agctatcaaa caaggagcaa ccgatgagtc tgttcaacaa agtcaccaag
     1801 tccttccagt ggggcgacaa gaccgtcgtc atggagacgg gcgagatcgc ccgccaggcc
     1861 acgggcgccg tgctggtcga tatcgacggc accgtcatcc tggccaccgt ggccgccagc
     1921 aagtcggcca agccggggca ggatttcttc ccgctcaccg tcgactacat cgagaaaacg
     1981 tacgccgccg gcaaaattcc cggcagcttc ttcaagcgcg aagccaagcc gagcgagctg
     2041 gagacgctca catcacgcct gatcgaccgc ccgatccgcc cgctgttccc cgacggcttc
     2101 ttcaacgaag tgcacgtcgt catccacacc ctgtcgctga accccgaggt cgatgccgac
     2161 atcgccgcca tgatcggcgt gagcgcggcg ctgtcgatca gcggcatccc gttcaacggc
     2221 cccatcggcg cggcgcgcgt gggctacgtc aacggcgaat acgtgctgaa ccccggcccc
     2281 accgcgcgca agagcagcgc gctggatctg gtcgttgccg gcactgaaag cgccgtgctg
     2341 atggtcgaat ccgaagccaa ccagttgacc gaggacgtga tgctgggcgc cgtggtgttc
     2401 ggccacgacc agggcaagat cgcaatcaac gccattcacg aactggtgcg cgaagccggc
     2461 aagccgctgt gggccgataa cggcacctgg gcgcccgaag tcaaggacga agccttcatc
     2521 ggcaaaatcg gtgcgctggc cgagccgaag ttgcgcgagg cctatcaaat ccgcagcaag
     2581 caagcccgca cgcaagcgct gcgcgaggcg actgccgccg tcaagcaggc gctgaccgaa
     2641 gaggccgtgg ccttcgatcc ggtcaagctc gacaacctgc tgttcgagat cgaagcgcgc
     2701 atcgtgcgtg gccagatcct ggcgggcgag ccgcgcatcg acggccgcga cacgcgcacc
     2761 gtgcgcccca tcgaaatccg caacagcgtg ctgccgcgca cccacggctc ggcgcttttc
     2821 acgcgcggcg agacgcaggc gctggtggtc accacgctgg gcaccgagcg cgatgcgcag
     2881 cgcatcgacg cgctggccgg cgagtttgaa gaccgctttt tgttccacta caacatgccc
     2941 cccttcgcca ccggcgaggc gggccgcatg ggcagcacca agcgccgcga agtcggccac
     3001 ggccgcctgg ccaagcgcgc gctgattccg ctgctgccgg ccaaggacga gtttccgtac
     3061 accatccgcg tggtgtcgga gatcaccgaa tccaacggct ccagctcgat ggcctccgtg
     3121 tgcggcggct gcctgtcgat gatggacgcc ggcgtgccga tgaaggcgca cgtggccggc
     3181 atcgccatgg gcctgatcaa ggaagacaac cgctttgccg tgctgaccga catcctgggc
     3241 gatgaagatc acctgggcga catggacttc aaggtggcgg gcaccaccgc cggtatcacg
     3301 gcgctgcaga tggacatcaa gatccagggc atcaccaagg aaatcatgca ggttgccctg
     3361 gcgcaggcca aggaggcgcg catgcacatc ctgggcaaga tgcaggaggc gatgggcgag
     3421 gccaaggccg agatcagctc gtgcgcgccc aagctctaca cgatgaagat caaccccgag
     3481 aagatccgcg acgtgatcgg caagggcggc gccac
//
LOCUS       DAOMVO010000259         3505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7816_length_3505_cov_13.328406, whole genome shotgun sequence.
ACCESSION   DAOMVO010000259 DAOMVO010000000
VERSION     DAOMVO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7816_length_3505_cov_13.328406"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKV30_10995"
     CDS             complement(<1..363)
                     /locus_tag="PKV30_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRL30771.1"
                     /translation="MNRPRAQRTASDSIAARARQTGASGPKDAESPVLPELRPGQSAL
                     LLKELHILTREGKLNQDSRRKLKQVGHLVQFIEKIVGELEPQGEQLTLADHGAGKSYL
                     GFILYDLLLKAQGSGTVYG"
     gene            complement(360..953)
                     /locus_tag="PKV30_11000"
     CDS             complement(360..953)
                     /locus_tag="PKV30_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HRL30772.1"
                     /translation="MRPVFTPDAAPPAIVIADTRRWLERAVIGLNLCPFAKAPHAKGQ
                     IHYAVCDSDDAADWLAQLRTELQALAALDATERETTLLMAPRLLPDFLDFNDFLAEAD
                     AVLAQLDLEGTLQIAPFHPRFQFAGTDEDDITNATNRAPYPTLHLIREASIDRAVAAF
                     PEAEAIYEANMATLARLGDEGWAALGVGASAPQGSAR"
     gene            complement(1042..2250)
                     /locus_tag="PKV30_11005"
     CDS             complement(1042..2250)
                     /locus_tag="PKV30_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL30773.1"
                     /translation="MATRPAPPHAGAAWIVILAGVSAALHVGKLPPALPVLRGELGIT
                     LVQAGFLLSLVQLASMTLGIVAGLMADGIGLKRCVVTGLALLSAAGLAGGFAHDATAL
                     LALRAAEGLGFLLTTVPAPSLVRRTVAPAAPTRMLGVWGSFMPFGTAVALLIGPLVMA
                     GFGWPAWWWLTAAFSLGMALWVAARVPSDPPRAKNAAADAAPWRQRLAQTLRAPGPWL
                     GALIFGVYSAQWLAVIGFLPSLYQQSGWGGARGAVLTALVAAVNMVGNIAAGRLLARG
                     VRPQALLWAGFGAMALGGFLAFGAATADSALLRYAGALLFSCVGGLIPGTLFALAPTL
                     APGERSISTTVGWMQQWSAVGQVSGPPLVAWVASQAGGWQFTWVVTGACCVAGALLAA
                     LIGRQPQRSA"
     gene            complement(2423..3496)
                     /locus_tag="PKV30_11010"
     CDS             complement(2423..3496)
                     /locus_tag="PKV30_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRL30774.1"
                     /translation="MAPAPTRDDFQRDRDRIVHSTAFRRLVYKTQVFLNHEGDLFRTR
                     LTHSIEVAQLARSIARALQLNEDLTEAIALAHDLGHTPFGHAGQDALNDCMAAHGGFE
                     HNLQSLRVVDQLEHRYPNHDGLNLTFETREGILKHCSPANARLLDAAEPGGVARRFID
                     GTQPSLEAQLANLADAIAYNAHDIDDGVRSGLLTLQQVAEVPLFARFVADTQRDHPGL
                     PDRRVLYESIRRMLSAQVYDVIDTTGAAIAAAVPPDADAARRAGPLVAFSAAMRAESN
                     ELKRFLFANLYRHPQVRDTTSQAQQVVRELFAAYLDRPAELRPSFAQRPDRERAVADY
                     IAGMTDRFALREHERLTGQKLLT"
BASE COUNT          555 a         1200 c         1252 g          498 t
ORIGIN      
        1 gccgtagacg gtgccgctgc cctgcgcttt cagcagcagg tcgtacaaga tgaagcccag
       61 gtacgacttg ccggcgccgt gatcggccag cgtgagctgc tcgccttggg gttcaagctc
      121 gcccacgatc ttctcgatga actgcaccag gtgcccgacc tgcttgagct tgcggcgcga
      181 atcctggttg agcttgccct cgcgcgtgag gatgtgcagc tctttcagca gcagggcgga
      241 ctggccgggg cgcagttcgg gcagcaccgg gctttcagca tcttttgggc cgctggcgcc
      301 tgtctggcga gcgcgagcag ctatcgaatc agaagcggta cgttgggcgc gcgggcgatt
      361 catcttgcgc taccctgcgg cgcgctggca ccgacgccga gcgcggccca gccttcatca
      421 cccaacctcg ccagcgtggc catgttcgct tcgtagatcg cctccgcctc cggaaaggcg
      481 gcgacggcgc ggtcgatgct ggcttcgcgg atcaggtgca gcgtggggta cggcgcccgg
      541 ttggtggcgt tggtgatgtc gtcttcatcg gtgccggcaa actgaaaacg cgggtggaag
      601 ggcgcgatct gcagcgtgcc ttcaagatcc agctgggcca gcacggcgtc ggcctcggca
      661 aggaagtcgt tgaaatcgag aaagtcgggc agcaggcgcg gcgccatcag cagcgtggtt
      721 tcgcgctcgg tcgcgtccag cgcggccagc gcctgcaact cggtgcgcaa ttgcgccagc
      781 cagtcggccg cgtcgtcgct gtcgcacacg gcgtagtgaa tctgcccctt ggcgtgcggc
      841 gccttggcaa acgggcacag gttgaggccg atcacggcgc gctccagcca gcggcgcgtg
      901 tcggcgatga cgatggctgg cggcgcggcg tcgggggtga aaacagggcg catggggcga
      961 ttgtgcctgc gcgaggcgcg acccgttgac tcctgattca atagctgctt gcgcaatcag
     1021 gacaagcgcc agcggccttt ttcatgcaga acgctgaggc tgccgcccaa tcaacgcagc
     1081 cagcaaggcg ccggcgacgc agcaggcgcc cgtcaccacc caggtgaatt gccagccgcc
     1141 cgcctgactg gcgacccagg ccaccagcgg cgggccgctg acctggccga cggccgacca
     1201 ctgctgcatc cagccgacgg tggtggagat gctgcgctca cccggcgcca gcgtgggcgc
     1261 cagtgcaaac agcgtgccgg ggatcagccc acccacgcat gaaaacagca gcgcgccggc
     1321 gtagcgcagc agcgcgctgt cggcggtggc ggcgccaaag gccaggaagc cgcccagcgc
     1381 catggcgccg aaacccgccc acagcaaggc ttgcggccgc acgccgcgcg ccagcagccg
     1441 cccggccgcg atgttgccga ccatgttgac cgccgccacc agcgccgtca gcaccgcgcc
     1501 gcgcgcgccg ccccagccgg actgttggta gagcgaaggc agaaagccga tcaccgccag
     1561 ccattgcgcc gaatagacgc caaagatgag cgcgccgagc cacgggccgg gcgctcgaag
     1621 cgtctgcgcc aggcgttggc gccacggcgc ggcgtcggcc gctgcgttct tggcgcgggg
     1681 tggatcggac ggaacacgcg cggccaccca aagtgccatc cccagcgaaa acgcggccgt
     1741 cagccaccac cacgccggcc agccgaagcc ggccatcacc aacggcccga tcaacagcgc
     1801 gacggcggtg ccaaagggca tgaagctgcc ccacacgccc agcatgcgcg taggcgcggc
     1861 gggggccacg gtgcgccgca ccaggctggg cgccggcacc gtggtcagca gaaagcccag
     1921 gccctcggcc gcgcgcagcg ccagcagcgc cgtggcatcg tgcgcaaaac cgcccgccaa
     1981 gccggccgcc gacagcagcg ccaacccggt caccacgcag cgcttgagcc cgatgccgtc
     2041 ggccatcaag ccggccacaa tgcccagcgt catcgacgcc agttgcacca gcgacagcaa
     2101 gaagcccgcc tgcaccagcg tgatgcccag ctcgccccgc agcaccggca gcgccggcgg
     2161 cagcttgccc acgtgcagcg cggcgctgac gccagcgagg atgacgatcc aagcggcgcc
     2221 cgcgtggggc ggcgcaggtc gtgtcgccat cacctgcgcc tgcccgcggc agcggacggc
     2281 acgatgaccg cgcctgcggc gcatgacctc ggcccgtggc cttcgatgac gcgccctgcg
     2341 ggcgcatgac tggcgtcatg gcgcgctgag cgcgccgtca tcgagcgcag cgaggtcatg
     2401 gcgcgccagt gccggtcatg catcatgtca gcagcttttg ccccgtcagc cgctcatgct
     2461 cgcgcagcgc gaagcgatcg gtcatgccgg cgatgtagtc ggccacggcg cgctcacggt
     2521 cgggccgctg tgcgaagctg gggcgcagct cggcgggccg atccaggtag gccgcgaaca
     2581 gctcgcgcac cacctgctgg gcttgcgacg tggtgtccct cacctgtggg tgccggtaca
     2641 ggttggcaaa caaaaaacgc ttgagctcgt tcgactccgc ccgcatcgcc gcactgaatg
     2701 ccaccagcgg tccggcgcgg cgcgcggcgt cggcatctgg cggcacggcg gccgcgatgg
     2761 cggcgccggt ggtgtcaatc acgtcgtaca cctgcgccga cagcatgcgg cggatggatt
     2821 cgtacagcac gcgccgatcc ggcaggccgg ggtggtcgcg ctgcgtgtcg gcgacaaagc
     2881 gcgcgaacaa gggcacctcg gcgacttgct gcagcgtcag caggcccgag cgcacgccgt
     2941 cgtcgatgtc gtgcgcgttg taggcaatgg cgtcggccag gttggccagt tgcgcctcca
     3001 ggctgggctg cgtgccgtcg atgaagcggc gcgccacgcc gccgggctcg gccgcgtcga
     3061 gcagtcgcgc gttggccggc gagcagtgtt tgaggatgcc ttcgcgcgtc tcgaaagtca
     3121 ggttcaggcc gtcgtggttc gggtagcggt gctccagctg atcgaccacg cgcaggcttt
     3181 gcaggttgtg ctcgaagccg ccgtgcgcgg ccatgcagtc gttaagcgcg tcttgcccgg
     3241 cgtggccgaa gggcgtgtgg ccgaggtcgt gcgcgagggc gatggcttcg gtcaagtcct
     3301 cgttgagctg cagcgcgcgg gcgatggagc gcgccagctg cgcgacctcg atcgaatgcg
     3361 tgagccgggt gcggaacagg tcgccttcgt ggttgaggaa cacctgcgtc ttgtagacga
     3421 gccggcgaaa agcggtcgag tggacgatgc ggtcgcggtc gcgctgaaag tcgtcgcgcg
     3481 tcggcgcggg cgcgatggcg tggcg
//
LOCUS       DAOMVO010000260         3499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7829_length_3499_cov_15.855110, whole genome shotgun sequence.
ACCESSION   DAOMVO010000260 DAOMVO010000000
VERSION     DAOMVO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_3499_cov_15.855110"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PKV30_11015"
     CDS             complement(<1..1133)
                     /locus_tag="PKV30_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HRL30775.1"
                     /translation="MVHHTISNSNTSAPSASSRIKVMKRASRFRFLAAALIVGMLASC
                     AVQPPPPTPAPLPPQRTEPALPLPGTVTDGAPIGPAIERARSRWVPVRWADLPGFEQD
                     NTTEAWNAWIKSCERPGPTFAPLCSEVRRLSLGTPDEQRQWMKARLQPYRVEALGAPT
                     EGLLTSYFEPMFDASRSARVGFGAPLYRPPAGLAQRKPWYTRQEMETLPEARAQLAGS
                     EIAFLADPVDALILQIQGSGRMRVTEADGSQRLVRLAFAATNDQPYRSPGKWLIDQGL
                     LRGDVTWPGIRNTLAANPQALQGFLWSNPRMVFFREEALSPLDAQFGPRGAQGVALTP
                     GRSIAVDPQSIPYGTPVWLSSQGPVANLQRLVLAQDTGSAIVGA"
     gene            1066..1230
                     /locus_tag="PKV30_11020"
     CDS             1066..1230
                     /locus_tag="PKV30_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30776.1"
                     /translation="MTLILLEALGALVLLLLIVWWTMFSGRKKGELPGQHDTPPRDDE
                     RAARKDASGD"
     gene            complement(1327..1953)
                     /locus_tag="PKV30_11025"
     CDS             complement(1327..1953)
                     /locus_tag="PKV30_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL30777.1"
                     /translation="MSRSDATVYIVDDDASVREALAWLLRSRRLLSESFDSAEAFEQA
                     LGQAEPAAPACLLLDVRMPGASGLALFDRLVERGVAHAWPVIFLTGHASVATAVEALK
                     RGAFDFCEKPFSDNALVDRVERALGVSAERVAQLAVQRALRARMGELTERERNVMRLV
                     ADGRPNKLIADELGISVRTVEVHRARVFEKMDVKSAVELSRALQQVPS"
     gene            2104..3294
                     /locus_tag="PKV30_11030"
     CDS             2104..3294
                     /locus_tag="PKV30_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HRL30778.1"
                     /translation="MSTPFLRRSFSLTPLLLAAALVACGGGDDDPATPEQPPENSAPG
                     ALLSGSMVNTITPAQITQAFKDPESHIQSGIVPRYSVSSYRLNYVTTDKNGAKVTASG
                     LVSVPVKAAGARSPVLSYQHASTFRNNQAPSIKVEAVEPPLVLASLGYIVVSPDYVGF
                     GASYGVEHPYLTSSPSSRAVIDMLSAAQTWRRQAGVVDNGQLFLAGYSEGGYATMAAH
                     RAIHQQGGELKTQLQAAVPGAGPYDVQETLDEQLDRVRKLFPPLALLIDPDHLSDASE
                     SVRNEVRRLLLRQMVPEDGDVRYQTLFMDRFLADDKAGIAAEHSVHLGWAPSVPVYLF
                     HGRGDLTVPYSAAESARDTLRAAGAPDVTLTDCTTPKFGHLDCVPEYFRFAVDRMGRL
                     AKDL"
BASE COUNT          541 a         1242 c         1193 g          523 t
ORIGIN      
        1 gcgccgacga tggcgctgcc ggtgtcttgc gccagcacca gccgttgcag attcgccacc
       61 ggcccctgcg acgacagcca caccggcgtg ccgtaaggaa tgctctgcgg atcgacggcg
      121 atcgagcgcc cgggcgtcag cgccacgccc tgcgcgccgc gcgggccgaa ttgcgcgtcg
      181 agcgggctca aggcctcttc gcggaagaac accatgcgcg ggttgctcca caaaaagccc
      241 tgcagcgcct gcgggttggc cgccagcgtg ttgcgaatgc cgggccaggt cacgtcgcct
      301 cgcagcaggc cctggtcgat cagccatttg ccggggctgc ggtagggctg gtcgttggtg
      361 gcggcaaagg ccagccgcac caggcgctgg ctgccgtcgg cctcggtcac gcgcatgcgg
      421 cccgagccct ggatctgcaa aatcagcgca tccaccgggt cggcgagaaa cgcgatctcg
      481 ctgccggcca gttgcgcgcg tgcctcgggc agcgtctcca tctcctgccg cgtgtaccag
      541 ggcttgcgct gcgccaggcc ggcgggcggc ctgtacagcg gcgcgccaaa gccgacgcgc
      601 gcgctgcgcg aggcgtcgaa catcggctcg aaatagctgg tcagcagccc ttcagtgggt
      661 gcgcccagcg cctcgacgcg gtagggctgc agccgcgctt tcatccactg gcgctgctcg
      721 tcgggcgtgc ccagcgacag gcggcgcacc tcgctgcaca gcggcgcgaa ggtggggccg
      781 gggcgctcgc agctcttgat ccaggcgttc caggcttcgg tggtgttgtc ctgctcaaag
      841 cccggcaaat cggcccagcg caccggcacc cagcggctgc gcgcgcgctc aatggccggg
      901 ccgatgggcg cgccgtcggt cacggtgccg ggcaacggca gcgccggctc ggtgcgctgc
      961 ggcggcagcg gtgcgggcgt ggggggcggt ggctgcacgg cgcagctggc cagcattcct
     1021 acaatcagcg ctgccgcaag aaaccgaaac cgtgatgctc gtttcatgac cttgattctg
     1081 ctggaagcgc tgggcgcgct ggtgttgctg ttgctgatcg tgtggtggac catgttttcg
     1141 ggccgcaaga aaggcgagct gccgggccag cacgacacgc cgccgcggga tgacgagcgc
     1201 gccgcgcgca aggatgcgtc gggcgattga gattcaaggc tttttaggcg ctggcgcacg
     1261 tcgggcaagc gcaggcagct atgaattatg cagcaaatca gtcggtggcg cttcgggccg
     1321 ccgcgcctac gagggcacct gctgcagcgc gcgcgacagc tcgacagcgg acttgacgtc
     1381 catcttctcg aacacgcgcg cgcggtgcac ttccaccgta cgcacgctga tgcccaactc
     1441 gtcggcgatc agcttgttgg gccggccgtc ggccaccagc cgcatcacgt tgcgctcgcg
     1501 ctcggtcagc tcgcccatgc gcgcgcgcag ggcgcgctgc accgccagtt gcgccacgcg
     1561 ctcggccgac acgcccaatg cgcgctccac gcggtccacc agcgcattgt ctgaaaaggg
     1621 tttctcgcaa aaatcaaagg cgccccgctt gagcgcctcg accgccgtcg ccacgctggc
     1681 gtgcccggtc agaaaaatca ccggccaggc gtgcgccacg ccgcgctcga ccaggcggtc
     1741 gaacagcgcc agtccgctgg cgcccggcat gcgcacgtcg agcagcaggc aggccggcgc
     1801 ggcgggctca gcctggccca gcgcctgctc gaaggcctcg gcgctgtcga acgattcgga
     1861 cagcagccgg cgcgagcgca gcagccaggc cagcgcctcg cgcacgctgg cgtcgtcgtc
     1921 gacgatgtag accgtggcgt cgctgcggga catggcgatg attctaggag cgcggcgccc
     1981 gaagccgcag cgccagacgc gcaaaagcgc ccctgtccga cagcgcgcgc ggcggcactc
     2041 tgcgatgctg cgcgtgatgg cggcacgacg gctgtgccgc gttcctcaac gtcgattcaa
     2101 gccatgtcca caccgttttt gcgccgcagc ttttcgctca cccccttgct tctggccgcc
     2161 gccctggtgg cgtgcggcgg cggtgacgac gaccccgcca cgcccgagca gccgcccgaa
     2221 aactcggcgc ccggcgcgct gctgtcgggc agcatggtca acaccatcac gccggcacag
     2281 atcacgcagg cgttcaagga tcctgaaagc cacatccaga gcggcatcgt cccgcgctat
     2341 tcggtgtcgt cgtaccggct gaactacgtc accaccgaca agaacggcgc caaggtgacg
     2401 gcctctggcc tggtcagcgt gcccgtcaag gcggcgggcg cgcgcagccc ggtgctgagc
     2461 taccagcacg ccagcacctt ccgcaacaac caggcgccca gcatcaaggt cgaggcggtg
     2521 gaaccgccgc tggtgctggc ctcgctcggc tacatcgtgg tgtcgcccga ctacgtgggc
     2581 tttggtgctt cgtacggcgt cgagcacccg tacctcactt catcgccgtc gtcgcgcgcc
     2641 gtcatcgaca tgctgagcgc cgcgcaaacc tggcgccgcc aggccggcgt ggtcgacaac
     2701 gggcagctct tcctggccgg ctattccgaa ggcggctacg ccaccatggc cgcgcaccgc
     2761 gccatccacc agcagggcgg cgagctgaag acgcaactgc aggctgccgt gcccggcgcc
     2821 ggcccgtacg acgtgcagga aacgctggac gagcaactcg accgcgtgcg caagctgttc
     2881 ccgccgctgg cgctgctgat cgaccccgat cacctgagcg acgccagcga gagcgtgcgc
     2941 aacgaggtgc gccgcctgct gttgcgccag atggtgccgg aggacggcga cgtgcgctac
     3001 cagaccctct tcatggaccg cttcctggcc gacgacaagg ccggcatcgc cgccgagcac
     3061 agcgtgcacc tgggctgggc gcccagcgtg ccggtgtacc tgttccacgg ccgcggcgac
     3121 ctgacggtgc cgtattcggc cgccgaatcc gcccgcgaca ccctgcgcgc cgccggcgcg
     3181 cccgacgtga cgctgaccga ctgcacgacc ccgaagttcg gccacctgga ctgcgtgccc
     3241 gagtacttcc gctttgcggt ggatcggatg gggcggctgg ccaaggattt gtgatggtcg
     3301 aacagcgaga cggctattga atcaatagct gctcgcgccc gtgtgacaag cgctggcggc
     3361 ctgtttcgtc atcaatcgac gggttcaggc ggcgtggcag cccgacccgt cagcgctcag
     3421 cggctcgacg gaaaactgaa caccgccccc tcgcgcacgc cggccgaagg ccagcgctgc
     3481 gtgatggtct tgcgcttgg
//
LOCUS       DAOMVO010000261         3495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7838_length_3495_cov_14.526453, whole genome shotgun sequence.
ACCESSION   DAOMVO010000261 DAOMVO010000000
VERSION     DAOMVO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7838_length_3495_cov_14.526453"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..805)
                     /locus_tag="PKV30_11035"
     CDS             complement(167..805)
                     /locus_tag="PKV30_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HRL30779.1"
                     /translation="MKLIAARACPACARAGFLSIFLLLGGCGALPLPDKPVRPQPYDL
                     GPALPQAASAAPSGPALALGRVDAPPAIDGSNIVYRLMYSDAGQQPRPYALARWTMAP
                     PQLLAQRLREAFSATRPVVEPGAGLAAVEMRTEIDEFAQIFSSPAASEGVVRLRVTVT
                     APGARAQRLLGQRTFTVRQPAPTADAAGGVAALRAASDEAVRQIVAWVNGLQ"
     gene            complement(815..1852)
                     /locus_tag="PKV30_11040"
     CDS             complement(815..1852)
                     /locus_tag="PKV30_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRL30780.1"
                     /translation="MENKSHALAAGAFVLAVAALLIGLAVWLMRDVTNTTAYEMVTDD
                     AVSGLQPQAVVRFKGVAVGKVTSIGFDRGNRANVLVRIAVAPDAPITKSTFATLTYQG
                     VTGLSFVQLDDAGGSTEPPPEGPNGVPRIPLKPNALGQLQDMASELAEKVGQATDRIN
                     QVLGPENQAALNTALVEIGGAAKSINQLASNTDKTIQAQFGAGKLDVPALVKQAGVSL
                     KSVETAAAQTRQTAANLNAVATQLGSGVERVTGPGGVVDQLSESAGTVTTNTLPRIQN
                     LTDDASRTIRRLDRIANSLAENPQSFIYGSGTIPPGPGEPGFGRAPAGGSAAPETPAP
                     AAPAPAPAVTR"
     gene            complement(1859..2722)
                     /locus_tag="PKV30_11045"
     CDS             complement(1859..2722)
                     /locus_tag="PKV30_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30781.1"
                     /translation="MSAPVMGQAPATLPPPGEPIIEVRGLWSAFPVPGGGQVVVHKDL
                     DLTVYRGEVLTLVGGSGTGKTVLLRQMLGLNTPYKGSITVLGQPVSQLTRDGAATRVG
                     MLFQQGALFSAFSVLDNIAFPLRETGALPDELVREAALLKLQMVGLKATDAHKMPSEL
                     SGGMIKRVALARALIMDPPLLMLDEPTAGLDPDSSDAFVTLLRALHQELGLTVVMVTH
                     DLDTLFELSTRVAVLADQHVIIAAPVADVLAFDHPFIQHFFLGERGQRAMELLHQRRD
                     GTPLTAPAKGD"
     gene            complement(2955..>3495)
                     /locus_tag="PKV30_11050"
     CDS             complement(2955..>3495)
                     /locus_tag="PKV30_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL30782.1"
                     /translation="TYIVNILGLALIRELGPLLAAVLIAGRSGSAITAQIGVMRVTEE
                     LDAMRVLGIPAGFRLVLPRAMALAIAMPLLSVWTTLASLIGGMLAADITLGITPAYFY
                     EALPKAVPLPNLTLACAKAVVFGVSIALIGCHYGLRVKPNTESLGQGTTASVVASITA
                     VILIDALFAVLFKSVGMSG"
BASE COUNT          525 a         1178 c         1269 g          523 t
ORIGIN      
        1 ccttagtgca acacggcgcg gtgcgcgcct cgccggaggc gctggcggcg ggtgccgacg
       61 cttgcgcggc gcatgcgctg gacgcgctgc tcgacccgca aaagcgcctg gcccggtttg
      121 ctggaggaaa tggaggaaga acacatggcg ctgtagcgcg cccgacctac tgcagcccgt
      181 tcacccacgc cacaatctgc cgcaccgcct cgtcgctcgc ggcgcgcagc gcagcgacac
      241 cgccagcggc atcggcggtg ggcgcgggct ggcgcacggt gaaggtgcgc tggcccagca
      301 ggcgctgcgc gcgcgcgccg ggcgcggtga cggtgacgcg caggcgcacc acgccttcgc
      361 tggcggcggg gctgctgaag atttgcgcga attcgtcgat ctcggtgcgc atctcgaccg
      421 ccgccagccc ggcgccgggc tcgaccaccg ggcgcgtggc cgagaacgcc tcgcgcaggc
      481 gctgcgccag caactgcggc ggcgccatgg tccagcgcgc cagcgcgtag gggcgcggct
      541 gctggccggc gtcgctgtac atcagccggt agacgatgtt gctgccgtcg atggcgggcg
      601 gcgcgtcgac gcggccgagc gccagcgcgg ggccgctggg cgcggcgctg gcggcctgcg
      661 gcagcgcggg gcccaggtcg tacggctgcg ggcgcaccgg cttgtcgggc aagggcagcg
      721 cgccgcagcc gcccagcagc aggaatattg aaagaaaacc ggccctggcg caggcaggac
      781 aagcgcgagc agctattaat ttcatatcaa accttcagcg ggtgacggcc ggtgcgggcg
      841 cgggtgcggc gggcgcgggg gtttctggcg cggcgctgcc gccggcgggg gcgcggccaa
      901 agccgggctc gccggggccg ggcgggatgg tgccgctgcc gtagatgaag gactgcgggt
      961 tttcggccag cgagttggcg atgcggtcga gccggcggat ggtgcggctg gcgtcgtcgg
     1021 tcaggttctg gatgcgcggc agcgtgttgg tggtgacggt gccggcgctt tcgctcagct
     1081 ggtcgaccac gccgccgggg ccggtcacgc gctcgacgcc gctgccgagc tgcgtcgcca
     1141 cggcattcag gttggcggcg gtctggcgcg tttgcgcggc ggccgtctcg actgatttga
     1201 gcgacacgcc ggcctgcttg accagcgccg gcacgtcgag cttgccggcg ccgaactgcg
     1261 cctgaatcgt cttgtcggtg ttgctggcga gctggttgat gcttttggcg gcgccgccga
     1321 tctcgaccag cgcggtgttc agcgcggcct ggttttcggg gcccagcacc tggttgatgc
     1381 ggtcggtggc ctggccgact ttttcggcca gctcgctggc catgtcctgc aactggccga
     1441 gcgcgttggg cttgagcgga atgcgcggca cgccgttggg cccttcgggc ggcggttcgg
     1501 tggagccgcc ggcgtcgtcg agctgcacga aggacaggcc ggtgacgccc tggtaggtca
     1561 gcgtggcaaa ggtcgatttg gtgatgggcg cgtcgggcgc gacggcaatg cgcaccagca
     1621 cgttggcgcg gttgccgcgg tcgaagccga tcgacgtgac cttgccgacg gccacgccct
     1681 tgaagcgcac cacggcctgc ggctgcaggc cgctgacggc gtcgtcggtc accatttcgt
     1741 aggcggtggt gttggtcaca tcgcgcatca gccacacggc cagcccgatc agcaaggccg
     1801 ccaccgccag cacgaaggcg ccggcggcga gggcgtggga tttgttttcc atgagcggtc
     1861 aatctccttt cgcgggcgcc gtcaacgggg taccgtcgcg gcgctggtgc agcagttcca
     1921 tggcgcgctg gccgcgttcg ccaaggaaga aatgctggat gaaggggtgg tcgaacgcca
     1981 gcacgtcggc caccggcgcg gcaatgatga cgtgctggtc ggccagcacg gcgacgcggg
     2041 tcgacagctc gaacagcgtg tcgaggtcgt gcgtgaccat caccaccgtc agccccagct
     2101 cctggtgcag cgcgcgcagc agggtgacga aggcgtcgga gctgtcgggg tcgagcccgg
     2161 cggtgggctc gtcgagcatc agcagcggcg ggtccatgat gagcgcgcgc gccagtgcca
     2221 cgcgcttgat catgccgccc gacagctcag atggcatctt gtgcgcgtcg gtggccttca
     2281 ggcccaccat ctgcagcttg agcagcgccg cctcgcgcac cagttcatcc ggcagcgcgc
     2341 cggtttcgcg cagcggaaag gcgatgttgt cgagcacgct gaaggccgag aacagcgccc
     2401 cctgctgaaa cagcatgcct acgcgcgtcg cggcgccgtc gcgcgtcagc tggctcaccg
     2461 gctggccgag cacggtgatg ctgcctttgt agggcgtgtt gaggcccagc atctggcgca
     2521 gcaacaccgt cttgccggtg cccgagccgc cgaccagcgt cagcacctcg ccgcggtaca
     2581 cggtcaagtc cagatccttg tgcaccacca cctggccgcc gcccggcacc gggaaggcgc
     2641 tccacaagcc gcgcacctcg atgatcggct cgccgggagg tggcaaggtc gcgggcgctt
     2701 gacccatcac cggcgcgctc atgcggccct cccaccgatg ccccgacgca cgttgccatt
     2761 cgaaacacta ttaaataaat agctgctcgc gcttttccag ccagcaccgg tggccgtttt
     2821 attcacaaaa ccaccagcac acaccatctg ggggccgcgg agcaggccat atgggaacgc
     2881 cctgcccggg gttcccccgg gcaatggcgt tgtccccccg gggggaaggc gccgcaggcg
     2941 actcaggggg gggctcaccc actcatcccc acgctcttga acagcacggc aaacagcgca
     3001 tcgatcagga tcaccgccgt gatcgacgcc accaccgacg ccgtggtgcc ctggcccagg
     3061 ctttcggtgt tcggcttcac gcgcaaacca tagtggcagc cgatcagcgc gatcgagacg
     3121 ccgaacacca ccgccttggc gcaggccagc gtcaggttgg gcagcggcac cgccttgggc
     3181 agcgcttcgt aaaagtaagc cggcgtgatg ccgagcgtga tgtcggccgc caacatgccg
     3241 ccgatcagcg aggccagcgt ggtccacacc gacagcagcg gcatggcaat tgccagcgcc
     3301 atggcgcgcg gcagcacgag gcgaaagccg gccggaatgc ccagcacgcg catggcgtcc
     3361 aactcttcgg tcacgcgcat gacgccgatc tgcgccgtga tggccgagcc ggagcgcccc
     3421 gcgatcagca ccgccgccaa cagcggcccc agctcgcgga tcagcgccag gccgaggatg
     3481 ttgacgatgt aggtc
//
LOCUS       DAOMVO010000262         3483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7880_length_3483_cov_22.437865, whole genome shotgun sequence.
ACCESSION   DAOMVO010000262 DAOMVO010000000
VERSION     DAOMVO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7880_length_3483_cov_22.437865"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKV30_11055"
     CDS             <1..807
                     /locus_tag="PKV30_11055"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRL30783.1"
                     /translation="LSRKMAALCDIYVNDAFGTAHRAEATTYGIAQFAPIASAGPLLA
                     AEIDALNQALASPKRPLVAIVGGSKVSTKLTILQSLADKVDQLIVGGGIANTFMLAAG
                     LPIGKSLAEPDLVDQARAVMAAMKARGAEVPIPTDVVTAKEFKPDAEAEVKAAQNVAL
                     DDLILDIGPDTAARLAAQLKKAGTIVWNGPVGVFELAPFENGTKTIAKAIAESPAFSI
                     AGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKTLPAFEILQKRAAG"
     gene            866..2440
                     /locus_tag="PKV30_11060"
     CDS             866..2440
                     /locus_tag="PKV30_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRL30784.1"
                     /translation="MLKTALRWTLIAFVVLALLSMLALWSWGRFAARAQGAPTTALAV
                     APAATALDRLVQPLLSRQPPDASGAAMLGEGVAAFVARAQAARVAERSLDVQYYIWRH
                     DLTGRLLEHELMAAAERGVRVRLLIDDMSVAGRDDALLAMDAHTNLEVRLFNPARNRA
                     SAVRRALEMGLRFAGFNRRMHNKAWIADNRVAIVGGRNVGDEYFDAADTNFHDADLLL
                     LGPAVAQTSDVFDAFWNSAAVVPLRALHQGGSRWSADEFSARRAQWWVDAKASPWVQA
                     LAGRDDLAEKLAPGGGLTVHWSPSIRVLSDPPEKASPLAHRQDRAGWLLYDVMALLFS
                     AQRDSWLISPYFVPGEGGTLLLAGQARRGVQVRVLTNSLAANDVPLVHAGYRDYRAPL
                     LAHGVQLYELKPGRRGPGRELIGSSGASLHTKAFVVDGARGFVGSFNFDPRSAQLNTE
                     MGVVFDHPALAAEVKRLFDAGTRPESAWRVTLDEGGALRWHGEGGQTWAREPEASAGL
                     RALVWLLSWLPIESQL"
     gene            2711..>3483
                     /locus_tag="PKV30_11065"
     CDS             2711..>3483
                     /locus_tag="PKV30_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRL30785.1"
                     /translation="MTHRATKIVATLGPASSDPALLEDMIRAGVNVVRLNFSHGTAAD
                     HIERARLVRAASERAGREVAIMADLQGPKIRVGKFEGGKVMLEPGAAFVLDAARKEPG
                     DVGGVGLDYRELPRDVKAGDTLLLNDGLIVLTVTEVRGDAVHTVVKLGGELSNNKGIN
                     KQGGGLTAPALTGKDMEDIKTAMGFQADYVAVSFPKSATDMEMARQLCTVAGTEHSHK
                     PQLIAKIERAEAIPRLTEILAASDGIMVARGDLAVEVGNA"
BASE COUNT          561 a         1202 c         1198 g          522 t
ORIGIN      
        1 ctttcgcgca agatggcggc gctttgcgac atttacgtca acgatgcctt cggcacggcg
       61 caccgcgcag aagccacaac ctatggcatt gcccagttcg cgcccatcgc cagcgccggc
      121 ccactgctgg cggccgagat cgacgcgctg aatcaggcgc tggccagccc caagcgcccg
      181 ctggtcgcca tcgttggtgg cagcaaagtg agcacaaaac tcaccatcct gcagtcgttg
      241 gccgacaaag tggatcaact cattgtcggc ggcggcatcg cgaacacctt catgctggcc
      301 gcgggcctgc ccatcggcaa aagcctggcc gagcctgatc tggtggatca ggcccgagcc
      361 gtcatggctg cgatgaaggc gcgtggtgcc gaagtgccga tcccgaccga cgtggtgacc
      421 gccaaggagt tcaagcccga cgcagaggcg gaagtcaaag cggcccagaa cgtggcgctc
      481 gacgacctga tcctggacat cggccccgac accgccgccc gcctggccgc gcagcttaaa
      541 aaagccggca ccatcgtctg gaacggcccg gtcggcgtgt tcgagctggc gccgttcgag
      601 aacggcacca agaccattgc caaagccatc gccgaatccc cagccttcag catcgccggc
      661 ggcggcgaca cgcttgcggc catcgccaag tacggcatcg acaaggacgt gggctacatc
      721 tccaccggcg gcggcgcgtt tctggaagtg ctggaaggca agacgctgcc ggcgttcgag
      781 attctgcaaa agcgcgcggc gggctgacgc gctgcgtttt cggtagctgc ctgcgcaatc
      841 cagacaagcg atgaagcctg ttttcatgct gaaaactgcc cttcgctgga cgctcatcgc
      901 cttcgtcgtg ctggcgttgc tgtcgatgct ggcgctgtgg tcgtggggcc gctttgccgc
      961 ccgcgcgcaa ggcgcgccga cgacggcgct ggccgtggcg ccggcggcca ccgcgctcga
     1021 tcgcctggtg cagccgctgc tcagccgcca gccgcccgac gccagcggcg ccgcgatgct
     1081 gggcgagggc gtggccgcct tcgtggcgcg cgcccaggcc gcgcgcgtgg ccgagcgcag
     1141 cctggacgtg cagtactaca tctggcgcca cgacctcacc ggccgcctgc tggagcacga
     1201 gctgatggcc gccgccgagc gcggcgtgcg cgtgcgcctg ctgatcgacg acatgagcgt
     1261 ggccggccgc gacgacgcgc tgctggccat ggacgcgcac accaacctcg aagtgcgcct
     1321 gttcaacccg gcgcgcaacc gcgccagcgc ggtgcgccgc gcgctggaga tgggcctgcg
     1381 ctttgccggc ttcaaccgcc gcatgcacaa caaggcgtgg attgccgaca accgggtggc
     1441 catcgtgggc gggcgcaacg tgggcgacga atacttcgac gccgccgaca ccaactttca
     1501 cgacgccgac ctgctgctgc tcggccccgc cgtggcgcag accagcgacg tgttcgacgc
     1561 cttctggaac tccgccgccg tggtgccgct gcgcgcgctg caccagggcg gctcgcgctg
     1621 gtcggccgat gaattcagcg cccgccgcgc ccagtggtgg gtcgacgcca aggccagccc
     1681 ctgggtgcag gcgctggccg ggcgcgacga cctggccgag aagctggcgc ccggcggcgg
     1741 gctcaccgtg cactggtcgc ccagcatccg cgtgctgtcc gacccgcccg agaaggcctc
     1801 gcccctggcg caccggcaag accgcgccgg ctggctgctg tacgacgtga tggcgctgct
     1861 gttttcggcc cagcgcgaca gctggctgat ctcgccctac ttcgtgccgg gcgagggcgg
     1921 cacgctgctg ctggccggcc aggcgcggcg cggcgtgcag gtgcgcgtgc tgaccaactc
     1981 gctggccgcc aacgacgtgc ccctggtgca cgccggctac cgcgattacc gcgccccact
     2041 gctggcccac ggcgttcaac tgtatgaatt gaaacccggc cgccgcggcc cggggcgcga
     2101 gctgatcggc tccagcggcg ccagcctgca caccaaggcc ttcgtggtcg atggcgcgcg
     2161 cggcttcgtt ggctcgttca acttcgaccc gcgctcggcc cagctcaaca ccgagatggg
     2221 cgtggtgttc gaccacccgg cgctggcggc cgaggtcaaa cgcctgttcg acgccggcac
     2281 gcggcccgag agtgcctggc gcgtgacgct ggacgagggc ggcgcgctgc gctggcacgg
     2341 cgaaggcggc cagacgtggg cgcgcgagcc tgaagccagc gcgggcctgc gcgcgctggt
     2401 gtggctgctg agctggttgc cgattgagtc gcagctctag attgcttctg tattgatagc
     2461 tgcttgcgct tgatggactg gcgccacgcg tcgatttgac cgagattgca ttacttcaaa
     2521 cagccggacg cgaaggacgc aaaagtgccg cgaaggacgc gaaagaattc aaaccgtttg
     2581 gttttccttt cgcgtccttc gcgaaccctt cgcgttcttc gcgtccggtc ttgatgacgg
     2641 gtctgtcgag cagtcacggc ccgtcacgcg cgcgtgccag aatgaccgcc tgctttttca
     2701 tcggaccgcc atgacccacc gcgccaccaa aatcgtcgcc accctcggcc ccgcgtccag
     2761 cgatcccgcg ctgctggagg acatgatccg cgccggcgtc aacgtggtgc ggctcaactt
     2821 cagccacggc acggcggccg accacatcga gcgcgcgcgc ctggtgcgcg cggcgtccga
     2881 gcgcgcgggg cgcgaggtgg ccatcatggc cgacctgcag gggccgaaga ttcgcgtcgg
     2941 caagttcgag ggcggcaagg tcatgctgga gccgggcgcc gccttcgtgc tggatgcggc
     3001 gcgcaaggag ccgggcgacg tcggcggcgt cgggctggat taccgcgaac tgccgcgcga
     3061 cgtgaaagcc ggcgacaccc tgctgctgaa cgacggcctg atcgtgctga cggtgaccga
     3121 ggtgcgcggc gacgcggtgc acacggtggt caagctgggc ggcgagctca gcaacaacaa
     3181 gggcatcaac aagcagggcg gcggcctgac cgcgccggcg ctgaccggca aggacatgga
     3241 ggacatcaag accgccatgg gctttcaggc cgactacgtg gcggtgagtt ttccgaaaag
     3301 cgcgaccgac atggaaatgg cgcgccagct gtgcaccgtg gccggcaccg agcacagcca
     3361 caagccgcag ctgattgcca aaatagaacg cgccgaggcg attccgcgcc tgaccgaaat
     3421 cctggccgcc agcgacggca tcatggtggc gcgcggcgac ctggcggtgg aagtgggcaa
     3481 cgc
//
LOCUS       DAOMVO010000263         3469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7916_length_3469_cov_15.032806, whole genome shotgun sequence.
ACCESSION   DAOMVO010000263 DAOMVO010000000
VERSION     DAOMVO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7916_length_3469_cov_15.032806"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKV30_11070"
     CDS             complement(<1..538)
                     /locus_tag="PKV30_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRL30786.1"
                     /translation="MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELASEVEQMLDDN
                     PFLECADDSAPREEFGLAHADTRVSEGDRAQENTTENIAAGADSASAEADFDAESALP
                     DSWDGDGSVEVRPDDGEWGGDAPARANNLGGDDELDPGERGAGQESLTEHLHRQALSL
                     RLSPEDTAALRFLIESLND"
     gene            complement(665..1423)
                     /gene="lptB"
                     /locus_tag="PKV30_11075"
     CDS             complement(665..1423)
                     /gene="lptB"
                     /locus_tag="PKV30_11075"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRL30787.1"
                     /translation="MAAEPGQNLLQVRGLRKNYGARRVVHDVSLDVRNGEVVGLLGPN
                     GAGKTTSFYMIVGLVRADAGSIAIDGQPVEHMPIHQRSRLGLSYLPQEASIFRKLTVA
                     ENVRAVLELQRGPDGRALPRAEIERRLTELLEQLHVNQLRDSPAPALSGGERRRVEIA
                     RALATQPRFILLDEPFAGIDPIAVIEIQRIIAFLKSRGIGVLITDHNVRETLGICDHA
                     VIISDGRVLATGTPTDIVADPEVRRVYLGEHFRL"
     gene            complement(1480..2130)
                     /gene="lptA"
                     /locus_tag="PKV30_11080"
     CDS             complement(1480..2130)
                     /gene="lptA"
                     /locus_tag="PKV30_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HRL30788.1"
                     /translation="MKPSVFSRACRLGLAAFCLLSASAAWAERADREKPMNIESDSLR
                     YEDQKQLSTFTGRVVVTKGTIVMRGARLEVRQDAAGNQFGTMFAEPGKRAFFRQKREG
                     LDEYIEGEGETIEYDGKADIVRFIRRAEMRRLAGATMVDQVLGSVIVYNNVTEVYTVD
                     GAPRSDGAASAAPGGRVRATLAPRNTGAAGAAPAAPASAPALPLRPAPALPPGAGQ"
     gene            complement(2252..2887)
                     /locus_tag="PKV30_11085"
     CDS             complement(2252..2887)
                     /locus_tag="PKV30_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HRL30789.1"
                     /translation="MKRREFTLGATLVAIPGLMTLPAHAQSGPGYVTLKERAPVDAPA
                     GQVEVVEFFSYGCSHCKNFEPTFQAWKKAAPKDVAVRMVHVGFQSSFEPLQRIYYTLE
                     TMGPAALDKVHAKVFEALQTERKRLDKPEVLFPWIAEQGIDRAKFEETYKSFGVASKV
                     RRAVQIQEAYHVEGTPALGIAGKYYTDGSMAGGFERMIQITNNLVAQEKKK"
     gene            complement(3105..>3469)
                     /locus_tag="PKV30_11090"
     CDS             complement(3105..>3469)
                     /locus_tag="PKV30_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRL30790.1"
                     /translation="PAPAVAAKPPAPAQLPSRAPSNDPLGELAAARAGTATAPAADPF
                     TYFVQAGAFRTQEDADGQRARLSLMGVEARVTEREQAGRTVYRVRVGPFQNKDAADRV
                     KDRLDGNGFDSALVRVQR"
BASE COUNT          524 a         1181 c         1193 g          571 t
ORIGIN      
        1 cgtcgttgag cgactcgatc agaaagcgca gcgccgccgt gtcctcgggc gacaggcgca
       61 gcgacaaggc ctgccggtgc aggtgctcgg tgagcgactc ctggccggcg ccgcgctcgc
      121 ccgggtccag ctcgtcatcc ccgcccaggt tgttggcgcg cgccggggcg tcgccgcccc
      181 attcgccatc gtccgggcgc acttcaacgc tgccgtcgcc gtcccagctg tcgggcaagg
      241 cggattcagc atcaaaatcg gcttcagcgc ttgctgaatc agcgccagca gctatgtttt
      301 cagtagtgtt ttcctgcgcc cgatcaccct cgctcacgcg cgtgtcggcg tgcgccagac
      361 cgaattcctc gcgcggcgcg ctgtcgtcgg cgcactccag aaaggggttg tcgtcgagca
      421 tctgctcgac ctcgctggcc agctccagcg ttgacaactg caacaagcgg atcgactgct
      481 gcagctgcgg cgtgagcgcc agatgctgcg agacgcgaag agacagcccg ggtttcacgt
      541 cacgttcatc atgagtcgag cggccgcgga gcaggccatg cgggggcgcc gtggagcggg
      601 cttggcccgg ccactggcgt cgtccccttc gggggaaggc gccgcaggcg acacaggggg
      661 gtcatcacaa cctgaaatgt tcgccgaggt agacgcgccg cacctcgggg tcggccacga
      721 tgtcggtcgg cgtaccggtg gccagcacgc ggccgtcgct gatgatcacc gcgtggtcgc
      781 aaatgcccag cgtttcgcgc acgttgtggt cggtgatcag cacgccgatg ccgcggcttt
      841 tgaggaaggc gatgatgcgc tggatctcga tcacggcaat cgggtcgatg ccggcaaacg
      901 gctcgtccag cagaatgaag cgcggctgcg tggccagtgc gcgagcgatc tcgacgcggc
      961 gccgctcgcc gcccgagagc gcaggcgccg gcgaatcgcg caactggttc acgtgcagtt
     1021 gctccagcag ctcggtcagg cgccgctcga tctcggcgcg cggcagggcg cggccgtcgg
     1081 ggccgcgttg caactccagc acggcgcgca cgttctcggc caccgtcagc ttgcggaaga
     1141 tcgaggcctc ttgcggcaga taagaaagcc ccaggcgcga gcgctggtgg atcggcatgt
     1201 gctcgaccgg ctggccgtcg atggcgatgc tgccggcgtc ggcgcgcacc aggccgacga
     1261 tcatgtagaa cgaagtcgtc ttgccggcgc cgttggggcc cagcaggccc accacttcgc
     1321 cattgcgcac gtcgagcgac acgtcgtgca ccacgcggcg ggcgccatag ttcttgcgca
     1381 ggccgcgcac ctgcagcagg ttctggccgg gctcagcggc cagcgcgccg ggcgcgctga
     1441 cggcaaaggt ggaggccagg tcgtcgatcg tggcctcgct cactggccgg cccccggcgg
     1501 cagcgcaggt gcggggcgca gcggcagtgc gggtgcggac gcgggcgcgg caggcgccgc
     1561 accggcggcc cctgtgttgc gcggcgccag cgtggcgcgc acgcggccgc ccggtgcggc
     1621 ggacgcggcg ccatccgagc gcggcgcgcc atccaccgtg tagacctcgg tcacgttgtt
     1681 gtagacgatc acgctgccca gcacctgatc gaccatggtg gcgccggcca gccggcgcat
     1741 ctcggcacgg cggatgaagc gcacgatgtc ggccttgccg tcgtattcaa tggtttcgcc
     1801 ctcgccctcg atgtattcgt cgaggccctc gcgcttctgg cggaagaacg cgcgcttgcc
     1861 gggctcggca aacatggtgc cgaactggtt gccggccgcg tcctgccgca cttccaggcg
     1921 cgccccgcgc atcacgatgg tgcccttggt caccaccacg cgcccggtga aggtcgacag
     1981 ctgcttctgg tcctcgtagc gcagcgaatc cgactcgatg ttcatcggct tttcgcggtc
     2041 ggcccgctcg gcccacgccg cgctggccga cagcagacaa aaagccgcca accccaggcg
     2101 acaggcacgg ctgaaaacgg aaggtttcat tgaaggcacg ttttttgctt gatggctgtt
     2161 ttgattgtac gctcggcgca ttcggcgctg ccgcacgccg cgcatgaaaa agcccgccga
     2221 agcgggcttt tggctgggct atcgccgaga gttacttctt tttctcctgt gccaccaggt
     2281 tgttggtgat ctggatcatg cgctcaaagc cgccggccat cgagccgtcg gtgtagtact
     2341 tgcccgcaat gccgagcgcc ggcgtgcctt cgacatggta ggcctcctgg atttgcaccg
     2401 cgcggcgcac cttgctggcg acgccaaagg acttgtaggt ttcctcgaac ttggcgcggt
     2461 cgatgccctg ttcggcgatc caggggaaca gcacctcggg cttgtccagg cgcttgcgct
     2521 cggtctgcag cgcctcgaac accttggcgt gcaccttgtc gagcgcggcc gggcccatgg
     2581 tctccagcgt gtagtagatg cgctgcagcg gctcgaagct gctctgaaag cccacgtgca
     2641 ccatgcgcac cgccacatcc ttgggcgccg ccttcttcca ggcctggaag gtcggctcga
     2701 agttcttgca gtgcgagcag ccgtacgaga agaactcgac cacctcgacc tgccccgccg
     2761 gcgcatcgac cggcgcgcgc tccttcagcg tcacatagcc aggccccgac tgggcgtggg
     2821 ccggcagcgt catcaaaccg ggaatggcca ccagcgtggc gcccaaggtg aattcacgac
     2881 gtttcatgtt ctcaagattc cgttgttaca agcctcagag accgggcgcc ggccgggagt
     2941 tccaaaagtt gcgcgcaacc cgtgacgaat tgcctatcgg ggctgcgcag caagccccaa
     3001 aaagggccgc ggagcaggtc attcgggaac gccgtggaac gggcttggcc cggccactgg
     3061 cgttgtcccc ctgggggaag gcgccgcagg cgccacaggg gggatcatct ctgcacgcgc
     3121 accagcgccg aatcaaaccc gttgccatcc agccggtctt tcacgcggtc ggcggcgtcc
     3181 ttgttctgga acgggccaac gcgcacgcgg tacaccgtgc ggccggcctg ctcgcgctcg
     3241 gtcacgcgcg cttcgacgcc catcagcgac aggcgggcgc gctggccgtc ggcgtcttcc
     3301 tgcgtgcgga aggcgccggc ctgcacgaaa taggtgaacg ggtcggcggc gggcgccgtt
     3361 gcggtgccgg cgcgcgcggc ggccaactcg cccagcgggt cgttcgacgg cgcgcgcgac
     3421 ggcaactgcg ccggcgcggg cggcttggcc gccaccgcgg gcgcaggcg
//
LOCUS       DAOMVO010000264         3448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7970_length_3448_cov_12.431771, whole genome shotgun sequence.
ACCESSION   DAOMVO010000264 DAOMVO010000000
VERSION     DAOMVO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7970_length_3448_cov_12.431771"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKV30_11095"
     CDS             <1..370
                     /locus_tag="PKV30_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRL30791.1"
                     /translation="TRPPVTSRQGMMRLASMVLLTIYSNLTEFSLHPTGDSMTSLTLS
                     DNGRVLIPAALREQLGFKPGGKIYAEVKDGSLVLTSPARHYAKLREYFDQHLPATPGR
                     SLVDEFIAERRAEAAREERE"
     gene            379..762
                     /locus_tag="PKV30_11100"
     CDS             379..762
                     /locus_tag="PKV30_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL30792.1"
                     /translation="MDSSVLLAVAKGEPGAEAVMELLHTQECVISSVNFAEVGTKLID
                     FGLPEAELKRGLAQFRLDVIDFDARQATACAALRAVTRAQGLSLGDRACLALASGMEG
                     VAVTADRAWADLGESVSGVRVLLIR"
     gene            complement(853..>1195)
                     /locus_tag="PKV30_11105"
     CDS             complement(853..>1195)
                     /locus_tag="PKV30_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30793.1"
                     /translation="VWDTQPQTSTAFQPPHFGAAIDRIHHVPPPTDDDTFNELQMTLT
                     APGRTLTLRQSDTLPDVVVWNPGPDAGVADLPGDEWRQFVCVEAAAVKLPVTLAPGAE
                     WIGWQAITLQR"
     assembly_gap    1196..1295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1296..1862)
                     /locus_tag="PKV30_11110"
     CDS             complement(<1296..1862)
                     /locus_tag="PKV30_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HRL30794.1"
                     /translation="MNLSSLFGGATEFTLQLPTGDRVRVSAHGGQVLHWSVDDGDELL
                     YLSPDASIDEGQAIRGGIPVCWPQFNTRGPLPKHGFARNAVWRLHGQDDTSLLLELTP
                     DDLSDEQRAAWSHQFQLRQKIDLAPGQLTVTLDVSNQGQTPFDFTTALHTYLRVADVT
                     QVQLAGLERTTVWDTQPQTSTAFQPPHFG"
     gene            complement(1894..3077)
                     /locus_tag="PKV30_11115"
                     /pseudo
     CDS             complement(1894..3077)
                     /locus_tag="PKV30_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(3084..>3448)
                     /locus_tag="PKV30_11120"
     CDS             complement(3084..>3448)
                     /locus_tag="PKV30_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRL30795.1"
                     /translation="IVGLDAKDGKVATDGWSKLTGHEVVDLAKKFEDWGVESIIYTDI
                     GRDGMLSGINIDATVKLAQALSIPVIASGGLSNMADIEALCAVESEGVEGVICGRAIY
                     SGDLDFAAAQARADELNG"
BASE COUNT          564 a         1122 c         1108 g          554 t
ORIGIN      
        1 gacgcggccg ccggtcacgt ccaggcaggg gatgatgcgt ttggcaagca tggttctttt
       61 gacaatttat tcaaatttga cagaattctc attacatcca acaggagact caatgacaag
      121 tcttacccta tccgacaacg gccgggtatt gatccccgca gcgctgcgcg agcaactggg
      181 cttcaaaccc ggcggaaaga tatacgccga agtgaaggac ggcagcctgg tgctgacctc
      241 gcccgcgcgg cactacgcaa aactgcgcga gtatttcgat cagcatttgc ctgccacgcc
      301 agggcgcagc ctggtggacg agtttattgc cgaacgccgc gccgaggccg cgcgcgaaga
      361 gcgcgaatga ttcatgtgat ggactcgtcg gtgctgctcg ccgtggccaa gggcgagccc
      421 ggcgccgagg cggtgatgga gttgctgcac acgcaggaat gcgtgatcag cagcgtcaac
      481 ttcgccgaag tcggcaccaa gctgatcgac tttggtttgc cagaggctga actgaagcgc
      541 ggcctggcgc agtttcgcct ggatgtgatc gacttcgacg ccagacaagc caccgcttgc
      601 gccgcgctgc gcgccgtgac gcgcgctcag ggcttgtcgc tgggcgaccg cgcctgcctg
      661 gcgctggcaa gcggcatgga gggcgtggcg gtcaccgccg accgcgcctg ggccgatctg
      721 ggcgaatcgg tgagtggcgt gcgcgtgctg ctgattcgct gatccgcttt ctgaattatt
      781 gaatcaaatc ggccgccagc gcttgccagt caagcgccag ctgctatcct ttcaagagta
      841 tcggacagaa actcaacgct gcagcgtgat cgcctgccag ccgatccact cggcacccgg
      901 cgccagcgtc accggcaatt tcaccgccgc cgcctccacg caaacgaact ggcgccactc
      961 gtcacccggc aaatcagcga cgcccgcatc cggccccggg ttccacacca ccacatcggg
     1021 cagcgtgtcg ctctggcgca gcgtgagcgt gcggccgggc gcggtcagcg tcatctgcag
     1081 ttcattgaat gtgtcgtcgt cggtgggcgg cggcacatgg tggatgcggt caatggcggc
     1141 accgaagtgc ggcggctgaa aagcggtgct ggtctgcggc tgcgtgtccc acaccnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccga agtgcggcgg ctgaaaagcg
     1321 gtgctggtct gcggctgcgt gtcccacacc gtcgtgcgct ccagcccggc cagctgcacc
     1381 tgcgtcacgt cggccacgcg caggtaggtg tgcagcgcgg tggtgaagtc gaacggtgtc
     1441 tggccttggt tgctgacgtc cagcgtcacc gtgagctgcc ccggcgccag gtcgatcttc
     1501 tggcgcagtt gaaactggtg cgaccacgcg gcacgctgtt cgtcgctcaa gtcgtcgggc
     1561 gtgagttcca gcaacaggct ggtgtcgtcc tgcccgtgca ggcgccagac ggcgttgcgc
     1621 gcaaagccgt gcttgggcag cggcccgcgc gtgttgaact gcggccagca gacggggatg
     1681 ccgccgcgga tagcctggcc ttcgtcaatt gaagcatcgg ggctcaggta cagaagctca
     1741 tcgccatcgt ccacgctcca gtgcagcacc tgaccgccat gggccgagac ccgtacgcgg
     1801 tcgccggtcg ggagttgcag cgtaaattca gtggcgccgc caaaaagcga tgagagattc
     1861 atgtgaaaca cagggcctaa aaaagcagcg cgctcagcgc tcgggccaat gccactgcgg
     1921 cgcatcgcac gcaccgtggc actgcgccac ggtttcgccg agcgattttt ccagctcgat
     1981 gcgctggcac acgtcagccg gcgccgcgtc ttcaatggcg gcgaccagcc gcatggcgtg
     2041 cgaaacgacg atcaactgcg tttcgcggct ggcgcgggca atcagcgcgc ccagcgccgg
     2101 cagcagatcg gggtgcaggc tggtttcggg ctcattcaac accatcagcg gcggcgggcg
     2161 cggcgtgagc agtgcgacgg cccacatcag aaagcgcagc gtgccgtccg acagctcggc
     2221 cgtgcgcagc gggcgcagca tgccgggctg ctgcatcgtc acctcgaagc gcccgtcgtc
     2281 gctgatctgc actgctactt gcgcgccgtc gaaggcatcc gccacggcgg cgtaaagcgc
     2341 gtcggcgtcg ccgatctcgt cgatggtttg cagcgcggcg gcaaagtcgt ggccgtcgcc
     2401 gctcagcacg ggcgtgcggc tgcccaattg cggcgcgcgc gcaggcgcct gcacgtcgca
     2461 gcgaaagtgg tcgtaaaacc gacacgcgcg cacggcgtcg cgcagcgtca gcatctcggg
     2521 cgcggcctgc gggtcggcgt attcggtcag catgctggtc cacggcggca gcacgcggcc
     2581 gacggtggcc cagacgcgac catcgcgcac gcgcagcgcg gcgccggcgc gctcgaccag
     2641 ccgcgcggcg ggacgcggca cggggccgct ccagatcact tcggtcttga cttcggggtc
     2701 gcggttgaag gccgatggag gtttgggcgg cggcaggccc aggtcgatgg cgtaactcaa
     2761 ggcatcgtcg cccgcgccgg caaagcccag tcgcaagctg atcgcccctt gcgcaccgtg
     2821 ccttgcaccg gcacttcgcc gcgccgcatg gcggccgtga tgttttcagg ccctgcccac
     2881 agcgtcgacg caaacccgcc ctcgcgcgcc agcgaggcca ccgccccgcc ctgcgccacc
     2941 tcggccagca gccgcagcgc acggtaaacg ctggacttgc cactgccatt gggcccagtg
     3001 acaatgttca gcggcgccag cggcagcacc acgtcgcgca gcgagcggta gttggcaatc
     3061 gccagcgcgg cgagcatgca gcgtcagccg ttcagctcgt ccgccctggc ctgcgcggcc
     3121 gcaaagtcca gatcgcccga ataaatggcg cgtccgcaga tcacgccttc aacgccctcg
     3181 ctctccaccg cgcacagcgc ctcgatgtcg gccatgttgc tcaggccgcc cgaggcgatg
     3241 acggggatcg acagcgcctg cgccagcttc accgtggcgt cgatgttgat gccgctcagc
     3301 atgccgtcgc ggccgatgtc ggtgtagatg atcgactcga cgccccaatc ctcgaacttc
     3361 ttggccagat cgaccacctc gtgcccagtg agtttgctcc agccgtcggt ggcgaccttg
     3421 ccgtctttcg cgtccaggcc gacgatga
//
LOCUS       DAOMVO010000265         3442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_7982_length_3442_cov_19.362858, whole genome shotgun sequence.
ACCESSION   DAOMVO010000265 DAOMVO010000000
VERSION     DAOMVO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7982_length_3442_cov_19.362858"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKV30_11125"
     CDS             complement(<1..788)
                     /locus_tag="PKV30_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL30796.1"
                     /translation="MKLGTFALAIAALGGIAAAPAVQAQAQEQFIPLLVYRTGQFAPL
                     GIPWADGKQDYLKLVNAKGGVNGVKIAFEECETAYDTAKGVECYERLKNKGSGASGFD
                     TQSTGITFATSDKAPGDKVPLVTMGYGLSQSADGRVFEWNFPLLGSYWTAADSMIQDI
                     LKKEKGSLKGKKIALVYHDSPYGKEPIPLLEKRAAKEGFELLKLPVTAPGVEQKSTWL
                     QIRQQRPDYVLLWSAGVMTPAAIREAQATNFPREKMYAIWWAGS"
     gene            complement(862..1926)
                     /locus_tag="PKV30_11130"
     CDS             complement(862..1926)
                     /locus_tag="PKV30_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL30797.1"
                     /translation="MFYRENGQFKTTYRADQQIFPILQDRMAMLALLVVAFFVVPLVA
                     SDYTFQAILIPFLIMSLAALGLNILVGYCGQISLGTGAFMAVGAYAAYNLQVRFDGMP
                     LLVSLLGGGLVAMVFGMVFGLPSLRIRGLYLAVATLAAQFFMDWLTSRAKWVTNDSSS
                     GSVSAGPLSILGWQIDTPMEKYLLCLAILCLIGLAAKNLVRSAIGREWMAIRDMDVAA
                     SVIGIRPMYAKLSAFAVSSFIVGVAGALWGFVHLGAWEPAAFSIDRSFQLLFMIIIGG
                     LGSIVGSIFGAAFFVLLPLLLNYVPHWLGLPLSTATATYLEHMIFGALIVFFLIVEPH
                     GLARLWATARQKLRVWPFPH"
     gene            complement(1992..2921)
                     /locus_tag="PKV30_11135"
     CDS             complement(1992..2921)
                     /locus_tag="PKV30_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL30798.1"
                     /translation="MGFFLETLLGGLMTGMLYALVAIGFVLIFKASGVFNFAQGAMVL
                     FAALAMARFSEWIPKWLGMAPGFVTLALALMVSVIIMIGVAWLIERLALRHLVNQEPI
                     TLLMATLGIAYLLDGLGPMIFGSAVYSIDVGMPKDPVFILENVFDGGVLVSKEDLYSA
                     CIAAVLVALLSLFFQKTKTGRALRAVADDHQAAQSIGIPLSRMWVIVWSVAGVVALVV
                     GVIWGSKIGVQFSLSLVALKALPVVILGGLTSLPGAIVGGLIIGVGEKLSEVYLGPMV
                     GGGIETWFAYGLALCVLLVRPQGLFGEKIIDRV"
     gene            complement(2941..>3442)
                     /locus_tag="PKV30_11140"
     CDS             complement(2941..>3442)
                     /locus_tag="PKV30_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30799.1"
                     /translation="LFKGMSVIDNIMSGRNLKFRSNILLQALRLGPAEREEIAQREFV
                     ERIIDFLEIQAYRKTPVGQLPYGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDM
                     CRFILDVNDEFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPDEVRSNEDVIRA
                     YLGTSH"
BASE COUNT          667 a         1133 c         1075 g          567 t
ORIGIN      
        1 tcggagccgg cccaccagat ggcgtacatc ttctcgcgcg ggaagttggt ggcctgcgct
       61 tcgcggatgg cggcgggcgt catcacgccg gccgaccaca gcagcacgta gtcggggcgc
      121 tgctggcgga tttgcagcca ggtcgatttc tgctcgacgc ccggtgccgt cacgggcagc
      181 ttgagcagct caaagccttc cttggccgcg cgcttttcca gcagcggaat cggctccttg
      241 ccgtagggcg agtcgtggta aaccagcgcg atcttcttgc ccttgaggct gcctttttcc
      301 ttcttcagga tgtcctggat catggaatca gcggccgtcc agtagctgcc cagcagcggg
      361 aagttccact cgaacacgcg gccgtcggcc gattgcgaca ggccatagcc catcgtcacc
      421 agcggcacct tgtcgccggg cgccttgtcg ctggtggcaa aggtgatgcc ggtcgattgc
      481 gtgtcaaagc ccgacgcgcc gctgcccttg ttcttcaggc gctcgtagca ttcgacgccc
      541 ttggcggtgt cgtaagcggt ctcgcattcc tcgaacgcga tcttgacgcc attgacgccg
      601 cccttggcgt tgaccagctt caggtagtcc tgcttgccgt cggcccatgg aatgcccagc
      661 ggcgcgaact ggccggtgcg gtagaccagc agcgggatga actgctcctg tgcctgtgcc
      721 tgaacggcgg gcgccgcggc gatgccgccc agtgcggcga tggcgagtgc gaaagtgccc
      781 agtttcatgg tgtgtctcct tgatgaattg acagggttgg gacagtgaat caacgcggca
      841 ggcgccgtgc ggacattggg gttaatgcgg aaatggccac acgcgcagct tctgccgtgc
      901 cgtggcccac aggcgcgcca ggccgtgcgg ctcgacgatc aaaaagaaca cgatcaacgc
      961 gccgaagatc atgtgttcca gataggtggc cgtggcggtc gagagcggca ggcccagcca
     1021 gtgcggcacg tagttgagca gcagcggcag cagcacgaag aaggcggcgc caaagatgct
     1081 gccgacgatg gagcccaggc cgccgatgat gatcatgaac agcagctgga acgagcggtc
     1141 gatggagaac gccgccggct cccacgcgcc caggtgcaca aagccccaca gcgcgccggc
     1201 cacgccgacg atgaagctgc tgacggcaaa cgcgctcagc ttggcgtaca tcgggcggat
     1261 gccgatcacc gacgcggcca cgtccatgtc gcggatcgcc atccactcgc ggccgatggc
     1321 gctgcgcacc aggttcttgg cggccaggcc gatcaggcac aggatggcca gacacagcag
     1381 gtacttttcc atcggcgtgt cgatctgcca ccccaggatc gacagcggcc cggcgctcac
     1441 cgagcctgac gacgaatcgt tggtgaccca cttggcgcgg ctcgtcagcc agtccatgaa
     1501 gaactgcgcg gccagcgtgg ccaccgccag gtacaggccg cgaatgcgca aggacggcag
     1561 gccgaacacc atgccgaaca ccatcgccac cagtccgccg cccagcagcg agaccaagag
     1621 cggcatgccg tcaaaacgca cctgcaggtt gtaggccgcg taggcgccca ccgccatgaa
     1681 ggcgccggtg ccgagcgaaa tctggccgca gtaaccgacc aggatgttca gccccagcgc
     1741 cgccagcgac atgatgagga acggaatcag gatggcctgg aaggtgtagt cgctggccac
     1801 cagcggcacc acgaagaaag ccaccaccag cagcgccagc atggccatgc ggtcctgcag
     1861 gatcggaaag atctgctggt cggcgcggta ggtcgtcttg aactggccgt tttctctgta
     1921 gaacatggtt tttggctcaa atcaggggtt ggtcggcgtg gatcaagcgc gagcagctat
     1981 cgaaaagata gtcaaacgcg gtcaatgatc ttctccccga acaggccttg cggccgcacc
     2041 agcagcacgc acagcgccag gccataggca aaccaggtct cgatgccgcc gcccaccatg
     2101 gggccgagat acacctccga cagcttctcg cccacgccga tgatcagccc gccgacgatg
     2161 gcgccgggca gcgaagtgag gccacccaga atcaccaccg gcaacgcctt cagcgccacc
     2221 agcgagagcg agaactgcac gccgatcttg gagccccaga tcacgcccac caccagcgcc
     2281 accacgcccg ccaccgacca cacgatgacc cacatgcgcg acaacggaat gccgatcgac
     2341 tgcgccgcct ggtggtcgtc ggccacggca cgcagggcgc ggccggtctt ggtcttctgg
     2401 aagaacagcg acagcagcgc caccagcacg gcggcgatgc aggccgaata caagtcttcc
     2461 ttgctgacca gcacgccgcc gtcaaacacg ttttccagaa tgaacaccgg gtctttcggc
     2521 atgccgacgt cgatgctgta gaccgcgctg ccaaagatca tcgggcccag cccgtcgagc
     2581 agatacgcga tgcccagcgt cgccatcagc agcgtgatcg gctcctggtt gaccaggtgg
     2641 cgcagcgcca gccgctcgat gagccaggcc acgccgatca tgatgatcac gctgaccatg
     2701 agcgccagcg cgagcgtgac aaagccgggc gccatgccga gccacttcgg aatccactcc
     2761 gaaaagcgcg ccatcgccag cgccgcaaac agcaccatgg cgccttgcgc gaagttgaag
     2821 acgccggaag ccttgaagat cagcacgaag ccgatggcca ccagcgcgta cagcatgccg
     2881 gtcatcaggc cgccgagcag ggtttcgagg aaaaagccca tgtgtttgtc tccgcggcgt
     2941 tcagtgactg gtgccgaggt acgcccggat cacgtcttcg ttgctgcgca cttcgtccgg
     3001 cgtaccgtcg ccgatcttct tgccgtagtc gagcaccacc acgcggtcgc tgatgtccat
     3061 caccacgccc atgtcgtgct cgatcagcac gatggtggtg ccgaactcgt cgttcacgtc
     3121 caggatgaag cggcacatgt cctgcttttc ttccacgttc atgccggcca tgggctcgtc
     3181 gagcagcagc acctgcggct ccatggccag cgctcttcct aagtcaaccc gcttttgcag
     3241 gccgtagggc aactggccca cgggcgtctt gcggtaggcc tggatttcga ggaagtcgat
     3301 gatgcgttcg acgaattcgc gctgcgcgat ttcttcgcgc tcggcgggcc ccaggcgcag
     3361 cgcctgcagc aggatgttgc tgcggaactt gaggttgcgc cccgacatga tgttgtcgat
     3421 cacgctcatg cccttgaaca gc
//
LOCUS       DAOMVO010000266         3404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8125_length_3404_cov_14.981786, whole genome shotgun sequence.
ACCESSION   DAOMVO010000266 DAOMVO010000000
VERSION     DAOMVO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8125_length_3404_cov_14.981786"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..528)
                     /locus_tag="PKV30_11145"
     CDS             complement(97..528)
                     /locus_tag="PKV30_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRL30800.1"
                     /translation="MTAETAAESGVRPADGYAGDLTPQTAHDWLQAGRAVLVDVRTDA
                     EREWVGFVPGAVAVAWKQWPGMALNADFDAQIRAAAQGGQPLMLLCRSGVRSIAAAKR
                     ATELGLKAYNVLGGFEGDPDAHGQRGHQNGWRHAGLPWRQN"
     gene            complement(958..1314)
                     /locus_tag="PKV30_11150"
                     /pseudo
     CDS             complement(958..1314)
                     /locus_tag="PKV30_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1356..1601)
                     /locus_tag="PKV30_11155"
     CDS             complement(1356..1601)
                     /locus_tag="PKV30_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRL30801.1"
                     /translation="MNTMAAEARRKALCARLARVEGQVRGLQRLIEADTDPEKVAQQM
                     AAARKALDKAFFAMVAGLIADGHTEADAIAELLVRFA"
     gene            1905..3016
                     /locus_tag="PKV30_11160"
                     /pseudo
     CDS             1905..3016
                     /locus_tag="PKV30_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          571 a         1109 c         1150 g          574 t
ORIGIN      
        1 gaagcaggcc atgcgcgaac gccgtggtcg gggtcccccc gaccaccagc gttgtcgccc
       61 tccgggggaa ggcgcgtcag cgccacaggg ggagcgtcag ttctgccgcc acggcagccc
      121 cgcgtgccgc cagccgttct ggtgcccgcg ctggccgtgc gcatcgggat cgccttcgaa
      181 accgcccagc acgttgtacg ccttcagtcc cagttcggtc gcgcgcttgg cggcggcgat
      241 ggagcgcacc ccgctgcggc acaacagcat cagcggctgg ccgccctgcg ccgccgcacg
      301 aatctgcgcg tcgaaatcgg cattcagcgc catgccgggc cactgcttcc aggccaccgc
      361 cacggcgccc ggcacaaagc cgacccattc gcgctcggcg tcggtgcgca cgtcgaccag
      421 caccgcgcgg ccggcttgca gccagtcgtg cgcggtctgc ggcgtcaggt cgccggcgta
      481 gccgtcggcc gggcgcacgc cggattcggc tgccgtctca gcggtcacgc agcctgaatc
      541 ctgccgcaaa cccgagcgct ggttggcggg cacggcttcg tccatgcgcc gtggcttggg
      601 caactcaagc gcgcccatca acgcgatgaa ttcgtcgcgc gattggccgg ccagccgcgg
      661 gttggcggct ttctccatgc cgatggtgga ctgcgtgcgc tcgtggtagt cgtggcccgg
      721 ccagacgatg gtggtgtcgg gcagggtgaa cagtttttgc gtgatgctgt cgtacagctg
      781 gccgggatcg cccgattgaa agtcggtgcg gccacagccc tgaatcagca gcgtgtcgcc
      841 ggtgaacacg tggcgctcgt cgtggcgcag gtcggggcgc atctcgtggc ccagctcgac
      901 ttcggtgact tcggtcggcg gctcgacgtg cggatgcggc gcgcgctgcc agtaaaagca
      961 gacgctgccg gcggtgtggc cgggcgtgtg cagcacgcgc aattgctcgc ggccaaaggg
     1021 cacgctgtcg ccatccttga gttgcacggc ggcggtgccg acctcgcacc cggccggcgc
     1081 cgccagtcgg gcgcccgtca actcggccag gcgcgcggcg ctggtgatgt ggtcggcgtg
     1141 tgcatgggtt tccagcgccc acttcagcct tgcgcccaag cgctccagcg tggccaggtc
     1201 acggtcaagc tgctcgtcca ccgggtcgat gatgacggct gcgcgcgacg cctcgtccag
     1261 caggatgtag gtgtaggtgc tggaagcggg atcgaaaagc tgggtcgggt tcatgcgccc
     1321 agtttataga gcgcggtgcg cgcgcggcca cgagatcagg cgaaacgcac cagcaactcg
     1381 gcgatggcat cggcctccgt gtgcccatcg gcgatcagcc cggcgaccat ggcgaagaag
     1441 gccttgtcca gcgccttgcg cgctgccgcc atctgctggg cgaccttttc ggggtcggtg
     1501 tcggcctcaa tcaggcgctg caagccgcgc acctgcccct ccacccgcgc cagtcgggcg
     1561 cacaaggcct tgcggcgcgc ctctgcggcc atcgtgttca tgccgggctc catggggttg
     1621 agggtggggg agcaaagcgc tcgggtcgga tcgtacgccc gtcatgcctg cctgaccatc
     1681 ggccaatcgg ggtaccttgt tcaggggaag atgcgggtaa accattgctc attatttgag
     1741 tctcggagca gccagtcgat caatccatgc aggctgagat ccaggttcgt ctcccgcgcg
     1801 gcgcgccgga acccgcgcca tgcttggtac acaccctctg acggctgacg aagggcgtgg
     1861 ccccagatgg cgcggctttt ggttacaaat atggagactt gaaaatggcc caacgccccg
     1921 cctcgcctcg ccgccgcaat ctcatcaagg gtgcagccgt cgctgccggc gcgctgaccg
     1981 cgcccatggt gtccaatgcg caaaccacgt cgctgcgctt tcaaagcacc tggccagcca
     2041 aggacatctt ccacgagtac gcgcaggact tcgcgaaaaa agtcaacgac atggcgggcg
     2101 cccgcttgaa gatcgaagtg ttgcccgccg gcgccgtggt gccggcgttc cagttgctgg
     2161 aggccgtgag caagggcacg ctggacggcg ggcatggcgt gatcgcctac cactacggca
     2221 agaactcggc gctggcgctg tggggttccg gcccggcctt cggcatggac ccgaacatgg
     2281 tgctgacctg gcacaactat ggcggcggca aggcgctggt ggaggagatc tacaagagcc
     2341 tgaacatgga cgtcgtgacc tacctttacg gcccatgccg acgcagccgc tgggctggtt
     2401 caagaagccg gtggccaagg ccgccgacat gaagggcctg aagttccgca ccgtcggcct
     2461 ggcggtcgac gtgttcaccg acatgggcgt ggccgtcaac ccgctgcccg gcggcgagat
     2521 cgtgccggcg ctggaccgcg gcctgattga cgcggcagag ttcaacaacg cctcgtccga
     2581 ccgcgtgctg ggcttcgccg acgtggcgaa gaactgcatg ctgcagagct tccaccagag
     2641 cgccgaacag tttgaaatcc tgttcaacgg gccgaagtac cgcgcgctgc cgcaggagtt
     2701 gcgctccatc gtcgactacg ccgtgcaggc cgccagcgcc gacatgagct ggaaggcgat
     2761 cgatcgcaac tccaaggact acatcgagct gaaaaaagcc ggcgtgaagt tctacaagac
     2821 gccggacgcc atcctgcgtg cgcagctcgc ctcgtgggac aagaccgtgg ccaagaagtc
     2881 ggccgagaac ccgttgttca agaaggtgct ggattcgcag cgccagttcg cggagcgcgc
     2941 cggccagtgg cagaacgact attcggtcga tctgaagatg gcctacaacc actacttcag
     3001 cggcaagaag agctgatgcg gcgggggcgg cgctcgcccg gtcctgcatg ccagcgaaca
     3061 ggggcgctca cgagggcgcc cttgtcgtgt aagggctgcg aaggcactgc gagccgacca
     3121 ggccgaatct cgataactat tgtttgatag ctgcctgcgc tgatccagca agcgctgaga
     3181 gcatgaaaag cttgaaatct tccatcggcg gcgccgcgca cgcgcgcgcc aggagtttgg
     3241 tgaatgcaca acctgttgct cgccgttgac cggctttcca cctggatcgg caaggtgttt
     3301 gcctggacca tcgtcgggct gacgctgctg atcagctggg aagtgttttc ccgctatgtc
     3361 atgaacaagc cgcacgcctg gatgctggac gcgcagatca tgct
//
LOCUS       DAOMVO010000267         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8151_length_3398_cov_17.492073, whole genome shotgun sequence.
ACCESSION   DAOMVO010000267 DAOMVO010000000
VERSION     DAOMVO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8151_length_3398_cov_17.492073"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PKV30_11165"
     CDS             complement(<1..2026)
                     /locus_tag="PKV30_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HRL30802.1"
                     /translation="MSRFNVKKVAVLGAGVMGAQIAAHLVNCKVPVILFDLAAKEGPK
                     NGIALKAIENLKKLKPAPLGVAEDAALIEAANYDDDLKKLKGCDLVIEAIAERMDWKS
                     DLYHKIAPFVAKHAILASNTSGLSITQLSEALPEALRPRFCGIHFFNPPRYMVLVELI
                     ATPTTRPEVLDQLEAFVTTALGKGVVRAKDTPNFVANRIGIAGMLATMKEVEKFGLTF
                     DVVDDLTGKRLGRASSGTFRTADVVGLDTMAHVIKTLQDNLSAETDPFYDSFGTPPVL
                     AKLLELKNLGQKTGAGFYKKQGRDILRFDLAKGEYVPSGAKADEVYGRMLKKPAGERL
                     KLLRNAEGPQGQFLWAILRNSFHYAAVHLATIAETARDVDLAMRWGFGMKQGPFELWQ
                     EAGWLQVAKMIQEDIDAGKALSRAPLPEWVFKGPVADAGGVHTEQGSWNPTTAKFEPR
                     RVLPVYERQLFPELLLGEAGPRPDTAGKTLLENDAIRLWTLDDEIVIATLKTKMRAIS
                     PDAGEGLAQAVEMAEKDYQGLVIWPGDDPFSVGADLQAMLPGYVAGGAGLVDSMEKEL
                     QEMMLRLRYANVPVVSAIRGMALGGGCELAVYSSRRVATMESYMGLVEVGVGLVPGAG
                     GLAYIARRAAENAQASTDKDLLHFLTEGFTAAAMAKVGMSALDSRKLGYLL"
     gene            complement(2038..2412)
                     /locus_tag="PKV30_11170"
     CDS             complement(2038..2412)
                     /locus_tag="PKV30_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRL30803.1"
                     /translation="MTPIGTWRSIDDSTKEPKAQVVITESGGVLTGRIEKVLKKDADP
                     KAVCDKCTDDRKDKPMTGLEIIRGAKKAEGKDVWEGGRVLDPEKGSDYALRLTPIDGG
                     KKLEVRGSIGPFGRTQTWTRVQ"
     gene            complement(2505..>3398)
                     /locus_tag="PKV30_11175"
     CDS             complement(2505..>3398)
                     /locus_tag="PKV30_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRL30804.1"
                     /translation="GNQSLGLTEVAYQNALAYAKERLQMRSLSGPKAKDKEADPIIVH
                     PDVRKMLLTAKAYAEGGRAMSIYTAMLLDKALYHPDEKVRKDSDEIVGLLTPIIKAFL
                     TDNGFAATNHAMQVFGGHGYIHETGVEQFVRDARINMIYEGTNTVQSLDLLGRKILGN
                     QGATLKKFGKLIAALVEEEGVNEKMSEFINPLGYLADQMTKFTTEIGFKGFTNPDEVG
                     GASVDYLRVAGHLVFGYFFARMASVALKKKAEGSTDPFYTAKLQTARFYFARLFPETA
                     SLMRTARSGVANLLDTDAALA"
BASE COUNT          517 a         1088 c         1097 g          696 t
ORIGIN      
        1 acagcaggta gccgagcttg cgcgagtcga gcgcgctcat gccgaccttg gccatggccg
       61 ccgccgtgaa gccttcggtc aggaagtgca gcaggtcctt gtcggtactc gcctgcgcgt
      121 tctcggccgc gcggcgggcg atgtaggcca ggccgcccgc gccgggcacc aggcccacgc
      181 cgacttcgac caggcccatg tagctttcca tggtggcgac gcggcgcgac gaatacacgg
      241 ccagctcgca accgccgccc agcgccatgc cgcgaatggc ggagacgacc ggcacgttgg
      301 cgtagcgcag gcgcagcatc atctcctgca gctctttttc catcgaatcg accaggcccg
      361 cgccgccagc gacgtatccg ggcagcatgg cttgcaggtc ggcgccgacg ctgaaggggt
      421 cgtcgcccgg ccagatgacc aggccttgat agtccttctc ggccatctcg acggcctgcg
      481 ccagcccttc acccgcgtcg gggctgatgg cgcgcatctt ggtcttgagg gtggcgatga
      541 cgatttcatc gtcgagcgtc cagaggcgga tggcgtcgtt ttccagcagc gtcttgccgg
      601 ccgtatcggg gcgcgggccg gcttcgccca gcagcagctc ggggaacagc tggcgctcat
      661 acaccggcag cacgcggcgc ggctcgaact tggccgtggt cgggttccac gagccctgct
      721 cggtgtgcac gcctccggca tcggcaacgg ggcctttgaa cacccactcc ggcagcggcg
      781 ctcttgacag cgccttgccg gcgtcgatgt cttcctgaat catcttcgcg acttgaagcc
      841 agccggcttc ttgccacaac tcgaacggcc cctgcttcat gccgaagccc cagcgcatcg
      901 ccagatcgac gtcgcgcgcc gtctcggcaa tggtggccag gtgcacggcg gcgtagtgga
      961 agctgttgcg cagaatggcc cacaggaact ggccttgcgg gccttcggcg ttgcgcagca
     1021 gcttcaggcg ctcgccggcc ggcttcttca gcatgcggcc gtagacctcg tcggccttcg
     1081 cgccgctggg cacgtactcg ccttttgcca ggtcaaagcg caggatgtcg cggccctgct
     1141 tcttgtagaa accggcgccc gtcttctggc ccagattctt cagctccagc agcttggcca
     1201 gcacgggcgg cgtaccaaag ctgtcgtaaa acgggtcggt ctcggccgaa aggttgtcct
     1261 gcagcgtctt gatgacgtgc gccatggtgt ccaggccgac cacgtcggcg gttcggaagg
     1321 tgccactgga ggcgcggccc agacgcttgc cggtcaggtc atcgaccacg tcaaaggtca
     1381 ggccgaattt ctcgacctcc ttcatcgtcg ccagcatgcc ggcgatgccg atgcggttgg
     1441 cgacgaagtt gggcgtgtcc ttggcgcgca ccacgccctt gccgagcgcg gtggtgacga
     1501 aggcctcgag ctggtccagc acttccggcc gcgtcgtcgg cgtggcgatc agctccacca
     1561 gcaccatgta gcgcggcggg ttgaagaagt ggatgccgca aaagcgcggg cgcagcgcct
     1621 cgggcagtgc ctcgctcagt tgggtgatcg acaggcccga ggtgttgctg gccaggatgg
     1681 catgcttggc gacgaagggc gcgatcttgt gatacaggtc gctcttccag tccatgcgct
     1741 cggcaatggc ttcgatcacg agatcgcagc ctttgagctt cttcaggtcg tcgtcgtagt
     1801 tggcggcttc gatcagcgcc gcgtcttcgg ccacgcccag tggtgcgggc ttgagctttt
     1861 tcaggttctc gatggccttc agcgcgatgc cgttcttggg cccttccttg gcggccaggt
     1921 cgaacaggat cacgggcacc ttgcagttga cgagatgggc cgcaatctgc gcgcccatca
     1981 cgcccgcgcc gagcacggcg actttcttga cgttgaatcg ggacattctg tgttccttca
     2041 ctggacgcgg gtccaggttt gcgtgcgccc aaacggcccg atggagccgc gcacttcgag
     2101 cttcttgccg ccgtcgatgg gcgtcaggcg gagcgcgtag tcgctgcctt tttcggggtc
     2161 aagcacgcgg ccgccttccc acacgtcctt accttcggcc ttcttggcgc cacggatgat
     2221 ttcaaggccc gtcatgggct tgtccttgcg gtcatcggtg catttgtcgc aaaccgcttt
     2281 cgggtcggcg tctttcttca gcaccttctc gatgcggccg gtgagcacac cgccgctttc
     2341 agtgatgacg acctgcgcct tgggttcttt cgtgctgtcg tcgatgctgc gccaggtgcc
     2401 gatgggcgtc atctgcgcca atgccgcacc actgaacact acaaaaccaa tagctgccag
     2461 cgcttgtaca gcaagcgctg gggccgtttt tttcttgaat ttcatcatgc cagcgccgcg
     2521 tcggtgtcca gcagattcgc cacgcccgaa cgcgcggtgc gcatcaggct ggcggtttcg
     2581 ggaaacaggc gcgcgaagta aaagcgcgca gtttgcagct tggcggtgta gaacggatcg
     2641 gtgctgccct cagctttctt cttgagtgcc acgctggcca tgcgcgcgaa gaagtagccg
     2701 aacaccagat ggcccgcgac acgcaggtaa tcgacgctgg cgccgcccac ttcgtcgggg
     2761 ttggtgaagc ccttgaagcc gatttcagtg gtgaacttcg tcatttggtc ggccaggtag
     2821 ccgagcgggt tgatgaactc gctcatcttc tcgttcacgc cttcttcttc caccagcgcg
     2881 gcgatcagct tgccgaattt tttcagcgtc gcgccctggt tgcccaggat cttgcggccc
     2941 agcaaatcga gcgactgcac ggtgttggtg ccttcgtaaa tcatgttgat gcgcgcgtcg
     3001 cgcacgaact gctccacacc cgtttcatgg atgtagccgt ggccgccgaa cacctgcatg
     3061 gcgtgattgg tggcggcaaa gccgttgtcg gtgaggaagg ccttgatgat gggcgtgagc
     3121 aggccgacga tttcgtcgct gtccttgcgc accttttcgt cggggtgata cagcgccttg
     3181 tcgagcagca tggccgtgta gatcgacatg gcgcggccac cttcggcata ggccttggcg
     3241 gtgagcagca tcttgcgcac gtcggggtgc acgatgatcg ggtcggcctc cttgtccttg
     3301 gccttggggc ccgacaggct gcgcatctgc aggcgctcct tggcgtaggc cagcgcgttc
     3361 tggtaggcca cttcggtcag gcccagcgac tggttgcc
//
LOCUS       DAOMVO010000268         3394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8164_length_3394_cov_14.391734, whole genome shotgun sequence.
ACCESSION   DAOMVO010000268 DAOMVO010000000
VERSION     DAOMVO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_3394_cov_14.391734"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKV30_11180"
     CDS             <1..322
                     /locus_tag="PKV30_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL30805.1"
                     /translation="WLRYNGPDTSLSFEIMMDVLLIVVIGGMGTMYGAVIGSVLFLLA
                     QSYLQDLLRIGSEATAGIPLLPALLSPDRWLLWLGVLFVLSVYYFPTGVVGKLRARSE
                     RAAR"
     gene            370..2520
                     /locus_tag="PKV30_11185"
     CDS             370..2520
                     /locus_tag="PKV30_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HRL30806.1"
                     /translation="MNSFHPLALAAAAACALSACGGSGDDGDKAPVNAAPAGVFGIQT
                     RSYDGASDDLLTAGLGKAGLQGALPAYADPLHPTVAELRRAAMYTSYRGLIDTTTAGG
                     YGVLYGPNVTATGAVTTGDGKVAGTETLAVLADNAVTLMAQVPASFDPKNPCIVTATS
                     SGSRGVYGAISTGEWGLKRGCAVAYTDKGTGGAPHDLQNDTVPMVDGLRRATASVGAL
                     NPNSPFGDFVAFNAGLSASELTAFNTATPNRFAFKHAHSGRNPEKDWGQFTLAAVRLA
                     LYAINQQFGEKNSSGERLATFSAGNTLVIASSVSNGGGAAIAAAEQDTEGLIDGVAVS
                     EPAIEMPANAGITVQRGAVQVNTVGKNLIDFTTHANLYQACASLAPSLAGAPFGAAFG
                     ALYNSAALPIAANRCAALKQEGLLSAAANTTAQQAEESLDRLRSYGWEPESAVLTPSL
                     AAFEVSPAVAVTFANALARARVSDHLCGFSYAATATNGSVTTLAPAALADMAATGNGV
                     PPSAGINLVNNRAAQGPARDFLSFTATGVADWNLDGALCLRELIRGSSAAAQRLQAGM
                     RETQRNGNLRGKPAIIVHGRDDALLPVNHTSRPYYGLNKKAEGAASKLSYIEVTHAQH
                     FDGFIGLPAVLPGYDSRYVPLHVYLNRALDAVYEHLKNGKPLPASQVVRTVPRGGTAG
                     QAPAITSANVPDIATVPTPGNAIRYSTATVVIPD"
     gene            2550..>3394
                     /locus_tag="PKV30_11190"
     CDS             2550..>3394
                     /locus_tag="PKV30_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRL30807.1"
                     /translation="MPSSSHYLVCAGREIHYMDWRPDDGASRGTVIAWHGLARTGRDM
                     DELAAHLSARGYRVLCPDTVGRGLSQWASKPEREYCLAAYARLARDFVLQLGIEQCHW
                     VGTSMGGAIGMVAASGLFEPELRARILSLTLNDNAPELAATAIERIKAYAGQPPAFDR
                     VTELEAFLRTAYKPYGFLTDAQWRRLTETSTRRLPDGRVTPHYDPAIVQQFTHHPDDY
                     LLWSHYDALAVPVLLLRGAESDLVLRDTAERMTTRGPGARGQLRWIEVPGCGHAPALN
                     VPAQL"
BASE COUNT          539 a         1288 c         1098 g          469 t
ORIGIN      
        1 ctggctgcgc tacaacgggc cggacacctc gctcagcttc gaaatcatga tggacgtgct
       61 gctgatcgtc gtcattggcg gcatgggcac gatgtatggc gccgtcatcg gctcggtgct
      121 cttcttgctg gcgcaaagct atttgcagga cttgctgcgc atcggcagcg aagcgactgc
      181 cggcattccg ctgctgcccg ccctgctctc gcccgaccgt tggctgctgt ggctgggcgt
      241 gctgttcgtg ctctcggttt actacttccc gaccggcgtg gtaggcaagc tgcgcgcgcg
      301 cagcgagcgg gccgcgcgct gaagttgctc atcccctcaa acgcaaatca caacgacagg
      361 agacaagcca tgaactcttt tcaccccctt gcattggcgg ccgcagcggc ctgtgcgctg
      421 tcggcgtgcg gcggcagcgg cgacgatggc gacaaggcgc cggtcaacgc cgcgccggcc
      481 ggcgtcttcg gcatccagac ccgcagctac gacggcgcca gcgacgacct gctgacggcc
      541 ggcctcggca aggccggcct gcagggcgcg ctgccggcct acgccgaccc gctgcacccc
      601 acggtcgccg agttgcgccg cgccgccatg tacaccagct accgcggttt gatcgacacc
      661 accacggccg gcggctacgg cgtgctgtat ggccccaacg tcaccgcgac gggtgcggtg
      721 accaccggcg acggcaaggt cgccggcacc gagacgctgg ccgtgctggc cgacaacgcc
      781 gtcaccctga tggcccaggt gccggcgagc ttcgacccga aaaacccgtg catcgtcact
      841 gcgacttcgt cgggctcgcg cggcgtttat ggcgccatca gcaccggcga atggggcctc
      901 aagcgcggct gcgccgtggc ctacaccgac aagggcacgg gcggcgcgcc gcacgatctg
      961 cagaacgaca cggtgccgat ggtcgatggc ctgcgccggg ccaccgcgag cgtgggcgcg
     1021 ctaaacccca actcgccgtt cggtgacttc gtcgccttca acgcgggcct gagcgccagc
     1081 gaactgaccg cattcaacac cgccacgccc aaccgctttg ccttcaagca cgcgcactcg
     1141 ggccgcaacc ccgagaagga ctggggccag ttcacgctgg ccgccgtgcg cttggcgctg
     1201 tacgccatca accagcagtt cggcgaaaag aactcaagcg gcgaacgtct ggcgaccttc
     1261 agcgccggca acacgctggt gatcgcctcc agcgtgtcca acggcggcgg cgccgcgatt
     1321 gccgccgccg agcaggacac cgaaggcctg atcgacggcg tggccgtgtc cgagcccgcc
     1381 atcgagatgc ccgcgaacgc cggcattacc gtgcagcgtg gcgctgtgca ggtcaacacc
     1441 gtcggcaaga acctgatcga cttcaccacg cacgccaacc tgtaccaggc ctgcgcgtcg
     1501 ctggcgccgt cgctggccgg cgcgccgttt ggcgccgcct ttggcgcgct gtacaactca
     1561 gccgcgctgc cgatcgccgc caaccgctgc gcggcgctga agcaggaagg cctgctgagc
     1621 gcggcggcca acaccacggc gcagcaggcc gaagaatcgc tcgaccgcct gcgcagctac
     1681 ggctgggagc ctgaatcggc cgtgctcacg ccctcgctgg ccgccttcga ggtgtcgccc
     1741 gccgtggccg tgacctttgc caacgcgctg gcgcgcgccc gcgtcagcga ccacctgtgc
     1801 ggcttcagct acgccgccac ggccaccaac ggcagcgtca ccacgctggc gcccgccgcg
     1861 ctggccgaca tggccgccac cggcaacggc gtgccacctt cggccggcat caacctcgtc
     1921 aacaaccgcg cggcgcaagg gccggcgcgc gacttcctga gcttcaccgc caccggcgtg
     1981 gccgactgga acctggacgg cgcgctgtgc ctgcgcgagc tgatccgcgg cagcagcgcc
     2041 gccgcccagc gcctgcaggc cggcatgcgc gagacccaac gcaacggcaa cctgcgcggc
     2101 aagccagcca tcatcgtgca cgggcgcgac gacgcgctgc tgccggtcaa ccacacctca
     2161 cgcccctact acggcctgaa caagaaggcc gagggcgcgg ccagcaagct gagctacatc
     2221 gaagtcacgc acgcgcagca cttcgacggc ttcatcggcc tgccggccgt gctgcccggt
     2281 tacgacagcc gctacgtgcc gctgcacgtg tacctgaacc gcgcgctcga cgccgtgtac
     2341 gagcacctga aaaacggcaa gccgctgccg gccagccagg tggtgcgcac cgtgccgcgc
     2401 ggcggcaccg cgggccaggc gccggccatc accagcgcca acgtgcccga catcgccacc
     2461 gtgccaacgc ccggcaacgc catccgttac agcaccgcca ccgtcgtcat cccggactga
     2521 cgcgcgcgcc acccgccccg agaaagccca tgccgtcttc gtcccactac ctcgtctgcg
     2581 ccggccgcga gatccactac atggactggc gccccgatga cggtgcctcg cgcggcaccg
     2641 tcatcgcctg gcatggcctg gcgcgcaccg ggcgcgacat ggacgagctg gccgcccacc
     2701 tgtcggcgcg cggctatcgc gtgctgtgcc ccgacaccgt ggggcggggc ctcagccaat
     2761 gggccagcaa gcccgagcgc gaatactgcc tggccgccta cgcccggctg gcgcgcgatt
     2821 tcgtgctgca gctgggcatc gaacaatgcc actgggtcgg cacctcgatg ggcggcgcca
     2881 tcggcatggt ggcggcgtca ggccttttcg agcccgagct gcgcgcgcgc atcctgagcc
     2941 tgacgctgaa cgacaacgcg cccgagctgg cggccacagc catcgaacgc atcaaggcct
     3001 acgccggcca gccgcccgct tttgaccgcg taaccgagct ggaagcattc ttgcgcacgg
     3061 cgtacaagcc ctacggcttt ctgaccgacg cgcagtggcg acgcctgacg gagaccagca
     3121 cgcgccgcct gcccgacggc cgcgtgacgc cgcactacga cccggccatc gtccagcaat
     3181 tcacccacca ccccgacgac tacctgctgt ggtcgcacta cgacgcgctg gccgttccgg
     3241 tgctgctgct gcgcggcgcc gagtcggacc tggtgctgcg cgacaccgcc gagcgcatga
     3301 ccacgcgcgg ccccggcgcg cgtggccagt tgcgctggat cgaggtgccg ggctgcggcc
     3361 acgcgccggc gctgaatgtg ccggcgcagc tgga
//
LOCUS       DAOMVO010000269         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8183_length_3388_cov_15.604860, whole genome shotgun sequence.
ACCESSION   DAOMVO010000269 DAOMVO010000000
VERSION     DAOMVO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8183_length_3388_cov_15.604860"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="PKV30_11195"
     CDS             complement(<1..1742)
                     /locus_tag="PKV30_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30808.1"
                     /translation="MSKDPASNGGLLSKVVKFVKSPTTHWSDLDRPEGEGEDSESRLA
                     LKEMIERKRRNDFVRNREFDMLRKARRRETLKDAEGAVGGPSFYPSSQPANTGERART
                     IKKIDEIEAQMSTAWFKRNGGAAPVGAQGPDAGEAPAERPYDPTDPVPLARDVAAGPP
                     PPAFAPTVTLEQIAPLDFAQSPTQAPAPVAQASAPVPVPVPSARLLGEVPEFNVEVMA
                     AAKQDPEIEEAAIRFANGDAAGAEAVLLDLVAEGGSRREDVHTWLTLFDLYRCMGEAT
                     KFDDAAFGFAALFGRSAPQWAVVTEAASAPAPIAVASTAVSTGLFNWTCPSTVGTQSV
                     AALKATMDRHAPPWRIDWRYLKTVEPAALPALLDVLKRWGETPAQLKFLGAERLLQAL
                     SERSPTDDRSVDPEWWEARLALLRVLGEMDEFELVALNYCVTYEVSPPAWEEPKSSYR
                     ALAEDGQTLPPPSDADAEAPSVPPPTFGLTQPYAGTPGAIEPGVVKATLAGEYLGTAE
                     EALKPLVAAREATSFEVNCRQLLRVDFGAAGDLLNWSMEQQGRGHQVTFKQVNRLVAA
                     FFGVIGINDSARVML"
     gene            complement(1776..2528)
                     /gene="dksA"
                     /locus_tag="PKV30_11200"
     CDS             complement(1776..2528)
                     /gene="dksA"
                     /locus_tag="PKV30_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRL30809.1"
                     /translation="MKPAAAKSPASPKATPAKAPTARVAAKAPTTAAKPAAKTGAPAA
                     KPVAKAAPPAPAPVRSAVAKKAAASPSRAPAMPPMSAPKLLPMPTVAAPPVPIPVKKD
                     PKLANNWKTKPVDQLSDAEVIAMPDNEYMNATQLAFFRLKLVQLKDDILANAGQTAEN
                     LRDDTVVVPDPADRATIEEEHALELRTRDRERKLLKKIEQSIDRIDSGDYGYCDETGE
                     PIGVPRLLARPTATLSLEAQQRRELKQKMFGD"
     gene            complement(2799..>3388)
                     /locus_tag="PKV30_11205"
     CDS             complement(2799..>3388)
                     /locus_tag="PKV30_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL30810.1"
                     /translation="VSPQELEALQHRLKHMNPRAPQRVVHFGEVPIKEVFDLRGFNLN
                     AKLDIDPDFLAADEHDHDHHEHDHDHHGAHCDHPSHQHAHGHHHHHDDDVKSFVFRAE
                     RPFDPAKLEDFLGAIVNIYGPRMLRYKGVLWMKGTERKVIFQGVHQLMGSDLGPGWKE
                     GEPRTSKLVFIGIDLPRDILLQGLEQCLTEAETVG"
BASE COUNT          492 a         1121 c         1176 g          599 t
ORIGIN      
        1 agcatcaccc gcgccgagtc gttgatcccg atcacgccaa agaacgccgc caccagccga
       61 ttgacctgct tgaaggtgac ctggtggccc cgcccctgct gctccatcga ccagttcagc
      121 agatcgcctg cggcaccgaa atcgacccgc agcaactggc ggcagttgac ctcgaaagag
      181 gtggcttcgc gcgcggccac cagcggcttc agcgcctctt cggcggtgcc caggtactcg
      241 ccggccagcg tcgccttgac cacgccgggt tcgatggcgc cgggggtgcc ggcgtagggc
      301 tgcgtgaggc cgaaggtcgg cggcgggacg ctgggcgctt cggcgtcggc atcgctgggt
      361 ggcggcagcg tctgcccatc ctcggccagg gcgcggtaac tgctcttggg ctcttcccag
      421 gccggcggcg agacttcgta ggtgacgcag tagttgagcg ccaccagttc gaactcgtcc
      481 atctcgccca gcacgcgcaa cagcgccagg cgcgcttccc accactcggg gtcgacgctg
      541 cggtcgtcgg tcggcgagcg ctcgctcagc gcctgcagca gccgctcggc acccagaaac
      601 ttcagctgcg ccggtgtctc gccccagcgc ttgagcacgt cgagcaaggc cggcaaggcc
      661 gccggttcca ccgtcttcag ataccgccag tcgatgcgcc agggcggcgc atgacggtcc
      721 atggtggcct tgagcgcggc caccgattgc gtgcccacgg tggaagggca agtccagttg
      781 aagaggccgg tgctgacggc ggtcgatgcc actgcgatgg gcgccggcgc agaggctgct
      841 tcggtcacca ccgcccactg cggcgccgag cggccgaaca gggcggcaaa gccaaaggcg
      901 gcgtcgtcga acttggtcgc ctcgcccatg cagcggtaga ggtcgaacaa cgtgagccag
      961 gtgtgcacgt cctcgcgccg gctgccacct tcggccacca gatcgagcag caccgcctcg
     1021 gcccccgccg cgtcgccgtt ggcaaagcga atggcggctt cctcgatttc cgggtcctgc
     1081 ttggcggcgg ccatgacctc gacgttgaac tcgggcacct cgcccagcag gcgggcagag
     1141 ggcaccggaa ccggaaccgg cgcagacgcc tgcgcgacgg gtgccggcgc ctgggtcgga
     1201 ctttgcgcaa aatccagcgg cgcgatctgc tccagggtca cggtgggcgc aaacgctggc
     1261 ggcggcggcc cggctgccac gtcacgcgcc aacggcaccg gatcggtcgg gtcgtacggg
     1321 cgctcggccg gggcttcacc ggcgtcgggc ccctgcgcgc cgaccggcgc tgcgccgccg
     1381 ttgcgcttga accaggcggt cgacatctgc gcctcgatct cgtcgatctt cttgatcgtg
     1441 cgggcgcgct cgccggtgtt ggccggctgg ctgctggggt agaacgaggg gccaccgacg
     1501 gcgccttccg cgtccttcag cgtctcgcgc cgccgcgcct tgcgcagcat gtcgaattcg
     1561 cggttgcgca cgaagtcgtt gcggcgcttg cgctcgatca tctccttcaa ggccaggcga
     1621 gactcgctgt cttcgccctc gccctcaggt cggtcgaggt cggaccaatg ggtggtcggg
     1681 ctcttgacga acttgaccac cttcgacagc aaaccgccgt tggatgcggg atccttgctc
     1741 atggctgccg gtacctcctg cggggaacgc cccgatcaat cgccaaacat cttctgcttg
     1801 agctcacgcc gctgctgcgc ctcgagcgac agcgtggccg tgggccgtgc cagcaggcgc
     1861 ggcacgccga tcggctcgcc ggtctcgtcg cagtagccgt agtcgcccga gtcgatgcgg
     1921 tcgatcgatt gctcgatctt cttgagcagc ttgcgctcgc ggtcgcgcgt gcgcagctcc
     1981 agcgcgtgct cttcctcgat ggtggcgcgg tcggccggat cgggcacaac cacggtgtcg
     2041 tcgcgcaggt tttcggcggt ctggccggcg ttggccagga tgtcgtcctt caactgcacc
     2101 agcttcaggc ggaagaaggc cagctgcgtg gcgttcatgt actcgttgtc cggcatcgca
     2161 atgacttcgg catcgctcag ctggtcgacg ggcttggttt tccagttatt ggccagtttg
     2221 ggatcttttt tgaccgggat cggcaccggt ggcgcggcaa cagtgggcat gggcaacagc
     2281 ttgggtgcgg acatcggcgg catggccggt gcacgcgaag gggaagccgc cgccttcttg
     2341 gcaacggcgg aacggacggg agcgggcgca ggtggggcgg ctttggcgac aggcttggcg
     2401 gcgggcgcgc cggtcttggc ggccggcttg gccgcagtcg ttggcgcctt ggcggccaca
     2461 cgggcggtgg gcgccttggc aggcgtggcc ttgggggagg ccgggctctt ggcagccgct
     2521 ggtttcactt ccgtctcctg tcggcaaagg cgctgtgaag cgcggcagcc gaccgcatgg
     2581 cgagcagatg aagcagggtc gttttcatcc gataagcgcc ccttgttata gaccttcttg
     2641 ggcgacacat gcaaaccgcc tgccgggggc ggacggtgcg tgcaaaggcg gcgattgtaa
     2701 cgatccgcca gcgccatggc gcccgcgcca ccgcccaaag ccggcaattc gccggaattc
     2761 gccctgttgt ggggaaaatc gtgacgccgg gcgcgccatc agccgacggt ttcggcctcc
     2821 gtcaggcatt gctcgagccc ctgcagcagg atgtcgcgcg gcaggtcgat gccgatgaac
     2881 acgagcttgc tggtgcgcgg ctcgccctct ttccagcccg ggccgagatc gctgcccatc
     2941 agctggtgca cgccctggaa gatcaccttg cgctcggtgc ctttcatcca cagcacgccc
     3001 ttgtagcgca gcatgcgcgg gccgtagatg ttgacgatgg cgcccagaaa gtcctccagc
     3061 ttggcggggt cgaacggccg ctcggcgcgg aacacgaagc tcttgacgtc atcgtcgtga
     3121 tgatggtggt ggccgtgtgc gtgctgatgc gacgggtggt cgcaatgcgc gccatggtgg
     3181 tcatggtcgt gctcgtgatg atcatggtcg tgctcgtcgg ccgccagaaa atcggggtcg
     3241 atgtccagct tggcattcag gttgaagccg cgcaggtcga acacttcctt aatcggcact
     3301 tcgccaaaat gcaccacgcg ctgcggcgcc cgtgggttca tgtgcttcag gcggtgctgc
     3361 agcgcctcca actcctgcgg gctgacca
//
LOCUS       DAOMVO010000270         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8191_length_3386_cov_15.283098, whole genome shotgun sequence.
ACCESSION   DAOMVO010000270 DAOMVO010000000
VERSION     DAOMVO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_3386_cov_15.283098"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKV30_11210"
     CDS             <1..359
                     /locus_tag="PKV30_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ecotin family protein"
                     /protein_id="HRL30811.1"
                     /translation="QRHVIELPALPDEDAHKLELIGGKAMTVDCNSRGMDGRFEARDV
                     PGWGYSYWVLQSQGQVHSTMMMCPPGSAKPGFVQAEPLLVRYNSKLPVVVFVPEGMQL
                     RWRVWRAGATQEAPAR"
     gene            complement(547..1770)
                     /locus_tag="PKV30_11215"
     CDS             complement(547..1770)
                     /locus_tag="PKV30_11215"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HRL30812.1"
                     /translation="MKPSSARPSPLSRRSLLAASAATALLMLPGCGGGGDGGSAGVGT
                     GGTGSFTSGPIRGFGSIVVNGVRYDDAAAQVVGDGGAALARSDLRLGMVVDVSGSDVS
                     TATDGRRSATATSIGVRSEIEGPVSAINAAAGTLTVLGQLVQITPTTVFDHDLRGGLA
                     AVAVGMVVEVYGFLQPSGQYLATRIDDEDDPVTHYKLRGVVSGLDTAARTFRIGTALV
                     SYADIAASVSGLANGQYARVELQLTPDASGAWRARSIRLATTAIGMPAGDGVKAEIEG
                     YITAFTSDARFSVAGIVVDASAVGQLPPGLAVGRRVEVEGVLSGGVLVARSVEFEDDD
                     DDDDDEFEIEGVITSVSASARTFSVRGITVNYANARFKDGTAEQLAPGVKVEVEGQLA
                     SDGTTLLATEVEFDN"
     gene            complement(1803..2669)
                     /locus_tag="PKV30_11220"
     CDS             complement(1803..2669)
                     /locus_tag="PKV30_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HRL30813.1"
                     /translation="MSQTLDQPVLLSAMHQLLAPLTRLALARGLPYAALDELLRAALV
                     REARRLHADVPGHGLVSRVSTTTGLTRREVSRLLEAGDEAPLPAPHSLTGEVLVRWMA
                     DPLYQRQGRPLALPRTGDAPSFEALAQSVTRDVHPRTLLGELCRLELVTCNTDIDTVA
                     LKQPAFVPRADQAHMLSLLADNVGDHLRAAVDNVLGSGDAHFEQAVYADELSLQSVQA
                     LRPLIAAQWADLLGRMAPELERRIADDRTERRAQDQRVRIGFYSFAAPMAGAAPAQRS
                     CSQERGLTQPVR"
     gene            3178..>3386
                     /locus_tag="PKV30_11225"
     CDS             3178..>3386
                     /locus_tag="PKV30_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30814.1"
                     /translation="MNDLTLPQSLATDSDGLPLAAALRARRIGIRGARTHNLKNIDLD
                     IPKDQLVVITGLSGSGKSSLAFDTL"
BASE COUNT          520 a         1179 c         1181 g          506 t
ORIGIN      
        1 accagcgcca cgtgatcgag ctgccggccc tgcccgacga ggacgcccac aagctggagc
       61 tgatcggcgg caaggccatg acggtggact gcaacagccg cggcatggac ggccgcttcg
      121 aggcgcgcga tgtgcccggc tggggctaca gctactgggt gctgcaaagc caaggccagg
      181 tgcacagcac catgatgatg tgcccgcctg gcagcgccaa acccggcttc gtgcaggccg
      241 agccgctgct ggtgcgctac aacagcaagc tgccggtggt ggtgttcgtg cccgagggca
      301 tgcagctgcg ctggcgggtg tggcgcgcgg gtgccacaca ggaggcaccg gcacgctgag
      361 cggcgcgcac gccgcagtac actatatttt ttatagctgc tgccgctttg ttgacgcgga
      421 taatccgcgt tttttgcatt aatctcggtc tgcgtcgccg cttcggcagc cgcgctcgcc
      481 gacgaaaaaa agccccgcca cccgtcgggc gggtgccggg gcccaatgct ccagtgtgca
      541 atcgcgtcag ttgtcgaact cgacctcggt cgccagcaag gtcgtgccgt cgctcgcgag
      601 ctgcccttcg acctcgactt tcacgccggg cgcgagctgt tcggccgtgc cgtccttgaa
      661 gcgcgcgttg gcgtagttca ccgtgatgcc gcgcacgctg aacgtgcgcg cgctcgccga
      721 cacgctggtg atgacaccct cgatctcgaa ctcgtcgtcg tcgtcatcgt cgtcgtcctc
      781 gaactcgaca ctgcgcgcca ccagcacgcc gccggagagc acgccctcga cctccacccg
      841 ccggccgacg gccaggcccg gcggcagttg gccgacggcg ctggcgtcca ccacgatgcc
      901 ggccacgctg aagcgcgcgt cgctggtgaa ggcggtgatg tagccctcga tctcggcttt
      961 cacgccgtcg cccgccggca tgccgatggc ggtggtggcc aaacggatgc tgcgcgcgcg
     1021 ccaggcgccc gacgcatccg gcgtgagctg cagctcgacg cgcgcgtact ggccgttggc
     1081 caggcccgac accgaggccg cgatgtcggc gtaggacacc aaggccgtgc cgatgcggaa
     1141 ggtgcgcgcc gcggtatcca gcccgctgac cacgccgcgc agcttgtagt gggtgacggg
     1201 gtcgtcctcg tcgtcgatgc gggtggcgag gtattggccg gagggctgca ggaagccgta
     1261 gacctcgacc accatgccca cggccacggc ggccaggccg ccgcgcaggt cgtggtcgaa
     1321 caccgtggtg ggcgtgatct gcaccagctg gcccagcacg gtgagcgtgc cggcggcggc
     1381 gttgatggcc gacacggggc cctcgatctc gctgcgcacg ccgatcgacg tggccgtggc
     1441 gctgcgccgg ccgtccgtgg ccgtgctgac gtcggagccg ctgacgtcga ccaccatgcc
     1501 caggcgcagg tcactgcgcg cgagcgcggc gccgccgtcg ccgacgacct gggccgcggc
     1561 gtcgtcgtag cgcacgccgt tgacgacgat cgagccgaag ccgcggatcg gtccgctggt
     1621 gaacgagccg gtgccgccgg tgccgacgcc ggcggagcca ccgtcaccac cgccgccgca
     1681 acccggcagc atgagcaggg cggtggcggc gcttgcggcc agcaggctgc ggcgcgagag
     1741 gggcgatggg cgtgcggatg atggcttcat ggatgacctc cggtggggac aaggaaacaa
     1801 gatcaacgaa cgggctgcgt cagcccgcgc tcttgcgagc acgagcgctg cgccggggcg
     1861 gcgccggcca tcggcgccgc gaatgaataa aagccgatcc gaacccgctg gtcctgcgcg
     1921 cggcgctcgg tccggtcgtc ggcgatgcgc cgctcgagct cgggcgccat gcggccgagc
     1981 aggtcggccc actgcgcggc gatcagcggg cgcagcgcct gcaccgattg cagcgacagc
     2041 tcgtcggcgt acaccgcctg ctcgaaatgc gcgtcgccgc tgcccagcac gttgtccacg
     2101 gcggcgcgca ggtggtcgcc cacgttgtcg gccagcagcg acagcatgtg cgcctggtcg
     2161 gcgcgcggca cgaaagccgg ctgcttgagc gccacggtgt cgatgtcggt gttgcacgtc
     2221 accagctcga gccggcacag ctcgcccagc agcgtgcgcg gatgcacgtc gcgcgtcacc
     2281 gactgcgcca gcgcctcgaa gctgggcgcg tcgccggtgc gtggcaacgc cagcggacgg
     2341 ccctggcgct gatacagcgg gtcggccatc cagcgcacca gcacctcgcc tgtcaggctg
     2401 tgcggtgcgg gcagcggggc ctcatcgccg gcctcgagca gccggctgac ctcgcgccgc
     2461 gtcaggcccg tggtggtgct cacgcggctg accagcccat ggcccggaac atcggcgtgc
     2521 aggcgccgcg cctcgcgcac cagcgcggcg cgcagcaact cgtccagcgc cgcgtaaggc
     2581 agaccgcgcg ccagcgccag ccgcgtcagc ggcgccagca actggtgcat ggccgacagc
     2641 aggacgggtt gatcaagggt ttgagacatg actgcgatta tgccattaaa tgtgcaattt
     2701 gcacatttat accgcactgt tgcaatgcga acgttgccgt gacatggcgt gaccgcgaac
     2761 cccccgccat gaaaaggaca tcgtcgtgag gtgcattgcc gggggccatg ccgccgtggc
     2821 gcggttgaag cggattgcgg gcagacggtg ccgcaatccg caggcatcgc actggggccg
     2881 aatgacgcga tccgcatcat ctcggagccc aagctgctgc accgattcaa ccggaccgat
     2941 gcgccgaccc gagtgcgcac cggctccttt atgggtcgct tgccgcgcaa ttgaggtgct
     3001 ggctcaagcc ggatttggca ctgaatccgg cgcccacgat cgctgcatcg accattcact
     3061 ggcccgcatt caccgggccg gcggcaagct gtgcccaacg ggcgctcgga cagggcagcc
     3121 gccgggcgcc cgtcggctgc gtggtgtgcg tcgtatcccg ataatggtgg gttgcccatg
     3181 aacgacctga ctcttcccca atccctcgcc accgactcgg acggcctgcc gctggccgcc
     3241 gcgctgcgcg cccggcgcat cggcattcgc ggcgcacgca cgcacaacct gaagaacatc
     3301 gacctggaca tccccaagga ccagctggtg gtcatcaccg ggctgtcggg ttcgggcaag
     3361 tccagtctgg cgttcgacac gctgta
//
LOCUS       DAOMVO010000271         3376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8222_length_3376_cov_18.379103, whole genome shotgun sequence.
ACCESSION   DAOMVO010000271 DAOMVO010000000
VERSION     DAOMVO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8222_length_3376_cov_18.379103"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKV30_11230"
     CDS             <1..789
                     /locus_tag="PKV30_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HRL30815.1"
                     /translation="VQSNIPFQAALLAHPKFVSGDFNTGFIAEHYAHGFKAEDVPHAD
                     PHFLQALAAHLHMFYRNRASGLQGQFWEGYKRLPRREFTVCVLGEGGDNRYTNVSAGE
                     TPPGQPTPITLHLPEGDKTYRISIDNEIGGARVTGTVDGQPFTVQMARGAGKNPLAIR
                     VVHNGTQMDAIVMHPRTAELHQLMPYKAPPDMSKFVLSPMPGLLVQVAVQPGQEVKAG
                     ERVAVIEAMKMENVLFASADGVVKEVRAQKGDSLAVDQAIVEFQ"
     gene            811..1119
                     /locus_tag="PKV30_11235"
     CDS             811..1119
                     /locus_tag="PKV30_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRL30816.1"
                     /translation="MSHLLKLAIHATPGASRTEAAGAHGAALRVRLAAPPVDGKANQA
                     LLAWAAQAFGLAKSRVELLHGAAGRQKVLGVHFDSQAELQAAQALVARWRASGGDDDA
                     "
     gene            1433..2326
                     /locus_tag="PKV30_11240"
     CDS             1433..2326
                     /locus_tag="PKV30_11240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRL30817.1"
                     /translation="MPDVQSVAVLHRGHLAYEFYRAGVTPGSLQDVQSVTKSVLALLF
                     GAAQADGHVRGPGELVALRLPQLLREATDPRAQRLRFAHLLTMTAGWAGEQTAQRDRD
                     DDLRQIVRRPFVADPGTRFAYDNGAANLAALALANAVGQPLSAYAHQRLLAPLGIQRF
                     AWRQGALGRELGALGLSLTTRGMARIGELVLSEGQWAGRALLPAAFVRDATRRQSAGG
                     YPVGQPYGYLWWVVDGGRGRQGARPAVMASGYGGQWIFVEPALQAVVAVTSRRTPESA
                     ARGQGLTLIRRDILPALQRLR"
     gene            2400..3026
                     /gene="pncA"
                     /locus_tag="PKV30_11245"
     CDS             2400..3026
                     /gene="pncA"
                     /locus_tag="PKV30_11245"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HRL30818.1"
                     /translation="MNKLDDATVLIIVDAQYGFMPGGGLAVADGDAVVPVINRIAPRF
                     ANVVLTQDWHPADHISFAANHPGRAPFETITLPYGEQVLWPTHCVQGTRDAALHDDLR
                     VPQAQLIIRKGFHRDVDSYSAFMEADRRTSTGLAAYLKARGISKLYLCGLATDYCVAW
                     SALDARAAGFEATVIEDACRAIDLNGSLARAWADMAAAGVGRVQSTDI"
     gene            3107..>3376
                     /locus_tag="PKV30_11250"
     CDS             3107..>3376
                     /locus_tag="PKV30_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL30819.1"
                     /translation="MTRPFKVLGIQQIAIGGPDKQRLQTLWVDMFGLQKTGTFKSERE
                     NVDEDICAIGSGPFKVEVDLMQPLDPEKKPAVHTTPLNHIGLWIDD"
BASE COUNT          552 a         1127 c         1171 g          526 t
ORIGIN      
        1 gtgcaaagca acatcccgtt ccaggccgcg ctgctggcgc accccaagtt cgtgagcggc
       61 gatttcaaca ccggcttcat tgccgagcac tacgcgcacg gcttcaaggc cgaagacgtg
      121 ccgcacgccg acccgcactt cctgcaggcg ctggcggcgc acctgcacat gttctaccgc
      181 aaccgtgcca gcgggctgca gggccagttc tgggaaggct acaagcgcct gccgcggcgc
      241 gaattcaccg tgtgcgtgct gggtgagggc ggcgacaacc gctacaccaa cgtgtcggcg
      301 ggcgagacgc cgcccggtca gccgacgccc atcacgctgc acttgcccga gggcgacaag
      361 acctaccgca tcagcatcga caacgagatc ggcggcgcgc gcgtcaccgg caccgtcgac
      421 ggccagccct tcaccgtgca gatggcgcgc ggcgcgggca agaacccgct ggcgatccgc
      481 gtggtgcaca acggcacgca gatggacgcc atcgtcatgc acccgcgcac cgccgagctg
      541 caccagctga tgccctacaa ggcgccgccc gacatgagca agttcgtgct ctcgcccatg
      601 ccgggcctgc tggtgcaggt ggccgtgcag ccggggcaag aggtcaaggc cggcgagcgt
      661 gtggccgtga tcgaggccat gaagatggag aacgtgctgt tcgccagtgc cgacggcgtg
      721 gtgaaggaag tgcgggcgca aaaaggcgac agcctggcgg tggatcaggc catcgtggag
      781 tttcagtaag cgcccagctg cggccaccgc gtgagccatt tgctcaagct cgccatccac
      841 gccacgccgg gcgccagccg caccgaggcc gctggcgcgc acggcgccgc cttgcgcgtg
      901 cgcctggccg cgccgccggt ggatggcaag gccaaccagg cactgcttgc gtgggcggcg
      961 caggccttcg ggctggccaa gtcacgggtc gagttgttgc acggcgcggc ggggcggcag
     1021 aaggtgctgg gcgtgcactt cgattcacag gcggagctgc aggcggcgca agcgctggtc
     1081 gcgcgctggc gcgccagtgg tggtgacgat gacgcttgat cggctggctg cagcgcagca
     1141 ccaaactata aaaacaagag ctgactgcgc tttctggacg ggcgccagca cctctttttg
     1201 ttgtgagacg atggtgccat ctgcggccca cgatgtttca caggcgcgtc ggcgggcgct
     1261 ggtcggtgcc gcgttgtcgg ccggtgcgct gtgcctgcct gcgtcgcgat cctgggcgca
     1321 gcgcgcggca gtctcggcgg ttgacgatgt cgcctggccc gcggcaccag cggccacggc
     1381 agcgcagcac cagcgtgcgt ttgccggctg ggatgagcgc gttcgcacgc agatgcccga
     1441 cgtgcaaagc gtcgccgtgc tgcatcgcgg ccacctggcg tacgagttct accgcgcggg
     1501 cgtcacgcca ggcagcttgc aggacgtgca gtccgtcacc aagagcgtgc tggcgctgct
     1561 gttcggggcg gcgcaggccg atggtcacgt gcgcggcccc ggcgaactgg tggcgctgcg
     1621 cctgccgcaa ctgctgcgcg aggcgaccga cccgcgcgcg cagcgcctgc gcttcgcgca
     1681 tctgctgacc atgacggccg gctgggcggg cgaacagacc gcccaacgcg accgcgacga
     1741 tgacctgcgc cagatcgtgc gtcgcccctt cgtggccgac cccggcaccc gctttgccta
     1801 cgacaacggc gccgccaacc tggcggcgct ggcgctggcc aacgccgtcg gccagccttt
     1861 gtccgcatac gctcaccagc ggctgttggc gccgctgggc attcagcggt ttgcgtggcg
     1921 ccaaggcgcg ctggggcgcg agctgggcgc gcttggcctg tccctcacca cgcgcggcat
     1981 ggcgcgaatt ggcgaactgg tgttgagcga ggggcaatgg gcgggccgcg cgctgctgcc
     2041 cgccgcgttt gtgcgtgatg ccacccggcg ccagagcgcg ggcggctacc cggttggcca
     2101 gccgtacggt tatctgtggt gggtcgtcga tggcgggcgc gggcgccagg gagcgcggcc
     2161 tgcggtcatg gccagcggct atggcgggca gtggattttc gtcgagccgg cgctgcaagc
     2221 ggtggtggcc gtgacctcgc gccgcacgcc cgaaagcgcc gcgcgcggac agggtttgac
     2281 gctgatccgg cgcgacatcc tgccggcgct gcaacgcctg cgatgagagg atgctttgaa
     2341 aatcatagct gccagcgctt gattatcaag cactggtggc ctttttgatt gttgaaacca
     2401 tgaacaagct tgacgacgcc acggtattga tcattgttga cgcccaatac ggcttcatgc
     2461 ccggcggcgg cttggccgta gcggacggcg acgccgtcgt gcccgtcatc aaccgcatcg
     2521 cgccgcgctt tgccaacgtg gtgctcacgc aggactggca cccggccgac cacatctcgt
     2581 ttgccgccaa tcacccgggc cgcgcgccgt tcgagaccat cacgctgcct tatggcgagc
     2641 aggtgctgtg gcccacgcac tgcgtgcaag gcacgcgtga cgcggcgctg cacgacgacc
     2701 tgcgcgtgcc gcaggcgcag ctcatcattc gcaagggttt tcaccgcgac gtggacagct
     2761 attcggcctt catggaagcc gaccggcgca ccagcaccgg cctggccgcg tacctgaagg
     2821 cgcgcggcat cagcaagctg tatttgtgcg ggctggcgac cgactactgc gtggcctgga
     2881 gcgcgctcga tgcgcgcgcg gccggcttcg aagccacggt gatcgaagac gcgtgccgcg
     2941 ccatcgacct gaacggctcg ctggccaggg cctgggccga catggcggcg gctggcgtgg
     3001 ggcgcgttca gtccacggat atttaagtca aaccaggctg cagcgtttga tgggcaagcg
     3061 cgagcagcta tcaaatcaat actaaaccca ccgagaggaa caagccatga cccgtccctt
     3121 caaagttctc ggcatccagc agatcgccat tggcggcccc gacaagcagc gcctgcagac
     3181 gctgtgggtc gacatgttcg gcctgcagaa aaccggcacc ttcaagagcg agcgcgagaa
     3241 cgtggatgaa gacatctgcg ccatcggcag cgggccgttc aaggtcgagg tcgacctgat
     3301 gcagccgctt gaccctgaga agaagccggc cgtgcacacc acgccgctca accacatcgg
     3361 cctgtggatc gacgat
//
LOCUS       DAOMVO010000272         3348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8319_length_3348_cov_12.710295, whole genome shotgun sequence.
ACCESSION   DAOMVO010000272 DAOMVO010000000
VERSION     DAOMVO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8319_length_3348_cov_12.710295"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PKV30_11255"
     CDS             complement(<1..178)
                     /locus_tag="PKV30_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HRL30820.1"
                     /translation="MVGVFTFDAEARRMGGGKSMGRQSSNVTQREAARTPPAAPTNQA
                     GATAAGAGAAGTAAA"
     gene            complement(296..1030)
                     /gene="ubiE"
                     /locus_tag="PKV30_11260"
     CDS             complement(296..1030)
                     /gene="ubiE"
                     /locus_tag="PKV30_11260"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRL30821.1"
                     /translation="MATTHFGYQSVDEQDKARRVRGVFDSVASRYDLMNDLMSAGLHR
                     AWKAYTVMVANVQPGQRVLDIAGGTGDLALAFAKKVGPTGQVVHTDINEAMLRTGRDR
                     LLDAGVILPTTVCDAEKLPFPDGHFDLVSVAFGLRNMTHKDAALREMARVLKPGGKLL
                     VLEFSQVAKPLRKAYDVYSFSVLPRLGQLVAGDADSYRYLAESIRMHPNQQELKALMK
                     SNGFGHVDFHNMAGGVVALHVGIKCG"
     gene            complement(1134..1541)
                     /locus_tag="PKV30_11265"
     CDS             complement(1134..1541)
                     /locus_tag="PKV30_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRL30822.1"
                     /translation="MPAATGAPPQSMTVHAKSRVLELVYLDGAHFRIPFELMRVYSPS
                     AEVQGHGPGQEVLQTGKRAVEMTALAPVGNYAVQPSFSDGHDSGIFTWDYLYFLGSQQ
                     DELWREYEAKLAAAGVDRDAPMAAKGGGACGHH"
     gene            1631..2755
                     /locus_tag="PKV30_11270"
     CDS             1631..2755
                     /locus_tag="PKV30_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRL30823.1"
                     /translation="MITPALRRAVFLLLLALVTVAFFWIISPFFGAVFWAMVLALMFM
                     PVHRRLCARLRGRDTLAALGTLLFCMVIVVVPMIFVVGAMVDEATSFTQRLRTGEFNP
                     RTYFEQIQNALPGWSRDLLGRFGLFNAQDVVDKLTAAVVQGGQALTTRALAIGQNTLM
                     LLVNLGIMLYLLFFFLRDGRDLAQTIRRAVPMQRQHTDFLLSKFATVVRATVKGTVVV
                     ALVQGLLGGVAFAFLGIHGAVLWGVVMSVLSLLPAIGAALVWAPVAIYLIATGSMIEG
                     LGLAAWGVGVMGMVDNLLRPILVGKETKLPDYLVLLSTIGGLSIFGLNGFLIGPAIAA
                     LFVATWALFSHEEGATPEDAAVAVAAAPADESAPPPADGA"
     gene            complement(2762..>3348)
                     /locus_tag="PKV30_11275"
     CDS             complement(2762..>3348)
                     /locus_tag="PKV30_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HRL30824.1"
                     /translation="YLDIKTVVVSCGTCYDQIAGYEFDQIFPGSRIIDIHEYLLEKGI
                     TLPAAQQGAYLYHEPCHNPMKLGDSTKTVKALIGDNVFKNNRCCGESGTLGVTRPDIS
                     TQVRFRKEEEIRKGAAQLRADGLAGEKQNVKILTSCPSCLQGLKRYEDDLPNGGLLEA
                     DYIVIEMARHILGENWLKDYVDRANAGGIERVLV"
BASE COUNT          507 a         1088 c         1113 g          640 t
ORIGIN      
        1 cggcagcggc cgtgcccgcc gcacccgcgc cggccgcagt ggcacccgcc tggttggtgg
       61 gcgcggccgg cggcgtgcgc gcggcttcgc gctgggtcac gttgctggac tggcggccca
      121 tggatttgcc gccgcccatg cgccgcgcct cggcgtcaaa ggtgaacacg cccaccatca
      181 ccaccgccag cagcagcgtg aaaaggcgcg aaacgatgtg tttgagagtc atacttgcct
      241 gtcctccaag tgagagatgt gaacgtgcgg cccaaaagcc ctgtttaaag agaggctaac
      301 cgcatttgat gccgacgtga agagcaacca ccccacccgc catgttgtga aaatccacgt
      361 ggccgaagcc gttggacttc atcagcgctt ttagttcctg ctgattgggg tgcatgcgga
      421 tcgattcggc caggtagcgg tagctgtcgg cgtcgcccgc caccagttgc cccaggcgcg
      481 gcagcacgct gaacgaatag acgtcgtacg ccttgcgcag cggcttggca acctgcgaaa
      541 actccagcac cagcagcttg ccgccgggtt tgagcacgcg cgccatctcg cgcagcgccg
      601 cgtccttgtg cgtcatgttg cgcaggccaa acgcgacgct gaccagatcg aagtggccgt
      661 cagggaaagg cagcttctcg gcgtcgcaca ccgtggtcgg caggatcacg ccagcgtcca
      721 gcaggcggtc gcggccggta cgcagcatgg cctcgttgat gtcggtgtgc accacctggc
      781 ccgtggggcc gacctttttg gcaaacgcca gcgccaggtc gccggtgccg cctgcgatgt
      841 cgagcacgcg ctggcccggc tgcacgttgg ccaccatcac ggtataggcc ttccacgcgc
      901 ggtgcaggcc ggccgacatc aggtcgttca tcaggtcgta acggctggcg acggaatcga
      961 agacgccgcg cacgcggcgc gccttgtcct gctcgtcaac cgactggtag ccaaagtggg
     1021 tggtggccat ggcgattctt taatttgaat gaaatcgggc gctggcgctt gtctgacaag
     1081 cgcgagcagc tatgaaaata tgagtattcg gcacacggag cagcttgaca ccttcaatgg
     1141 tgaccgcagg ccccgccgcc cttggcagcc atcggtgcgt cgcggtcaac gccggcggcg
     1201 gccagcttgg cttcgtattc gcgccacaac tcgtcctgct gcgatcccag gaagtacagg
     1261 tagtcccagg tgaagatgcc gctgtcgtgg ccgtccgaga agctgggctg caccgcgtag
     1321 ttgcccaccg gcgccagcgc ggtcatttcc acggcgcgct tgccggtctg cagcacctct
     1381 tgccccggcc cgtggccctg cacctcggcc gagggcgagt acacgcgcat cagctcaaac
     1441 ggaatgcgaa agtgcgcgcc gtccaggtac accagttcca gcacgcgcga cttggcgtgc
     1501 acggtcatcg attgcggggg ggcgccggtg gcagcgggca tggcttgggg cagatcgggc
     1561 ttgtgaagcg gtcattgtgc cctgtgacgc ccatagcccg cccggcgcac gggtatagtc
     1621 agcgcccatc atgattaccc cggcgctgcg gcgcgctgtc tttctgctgc tgctggcgct
     1681 ggtcaccgtg gcgttcttct ggatcatttc gccgttcttc ggcgccgtgt tctgggccat
     1741 ggtgctggcg ctgatgttca tgccggtaca ccggcggctg tgcgcccggc tgcgcggacg
     1801 cgacacgctg gcggcgctgg gcacgctgct gttttgcatg gtgatcgtgg tggtgccgat
     1861 gatcttcgtc gtcggcgcca tggtggacga ggccaccagc ttcacccaga ggctgcgcac
     1921 cggcgaattc aacccgcgca cttacttcga gcagatccag aacgcgctgc ccggctggtc
     1981 gcgcgacttg ctgggccgct tcgggctgtt caacgcgcag gacgtggtgg acaagctgac
     2041 cgccgccgtg gtgcaaggtg gtcaggcgct gaccacgcgc gcgctggcca tcgggcagaa
     2101 cacgctgatg ctgctggtca acctgggcat catgctgtat ctgctgttct tctttttgcg
     2161 cgacgggcgc gatctggcgc agaccattcg ccgcgcggtg ccgatgcaaa ggcagcacac
     2221 cgatttcctg ctgagcaagt tcgccaccgt ggtgcgcgcc accgtcaagg gcaccgtcgt
     2281 ggtggccctg gtgcagggcc tgctgggcgg cgtggcattc gccttcctcg gcatccacgg
     2341 cgcggtgctg tggggcgtgg tgatgtccgt gctgtcgctg ctgccggcca tcggcgcggc
     2401 gctggtgtgg gcgccggtgg ccatttacct gatcgcgacc ggctcgatga tcgaaggcct
     2461 gggactggcg gcctggggcg tcggcgtgat gggcatggtg gacaacctgc tgcgcccgat
     2521 cctggtcggc aaagagacca agctgcccga ctacctggtg cttttgtcca ccatcggcgg
     2581 cctctcgatc ttcggcctca acggcttctt gatcggcccg gccatcgcag cgctgttcgt
     2641 ggcgacctgg gcgctgttca gccacgagga aggcgcgacg ccggaggacg cggcggtcgc
     2701 ggttgcggcc gcgcccgccg acgaaagcgc cccgccgccg gcggatggcg cctgagcacc
     2761 cttacaccag cacgcgctcg atgccgcccg cgttggcgcg gtcgacgtag tccttcagcc
     2821 agttctcgcc caggatgtgg cgcgccatct cgatgacgat gtagtcggct tcgagcaggc
     2881 caccgttggg caggtcgtcc tcgtagcgtt tcaagccctg caggcagctc gggcagctgg
     2941 tcaggatctt cacgttctgc ttttcgcccg ccaggccgtc ggcgcgcagt tgcgccgcgc
     3001 ccttgcggat ttcttcttcc ttgcgaaacc gcacctgcgt cgaaatatcg ggccgcgtca
     3061 cccccagcgt gccgctttcg ccgcagcagc ggttgttctt gaacacgttg tcgccgatca
     3121 gcgccttcac cgtcttggtg gagtcgccca gcttcatcgg gttgtggcag ggctcgtggt
     3181 acaggtacgc accctgttgc gccgctggca gcgtgatgcc tttctccagc aggtactcat
     3241 gaatgtcgat gatgcggctg ccaggaaaaa tctggtcgaa ttcgtagccc gcaatctggt
     3301 cgtagcaggt gccgcagctc accaccacgg tcttgatgtc gaggtagt
//
LOCUS       DAOMVO010000273         3333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8367_length_3333_cov_12.467358, whole genome shotgun sequence.
ACCESSION   DAOMVO010000273 DAOMVO010000000
VERSION     DAOMVO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8367_length_3333_cov_12.467358"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKV30_11280"
     CDS             <1..209
                     /locus_tag="PKV30_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRL30825.1"
                     /translation="PAPVVAPARLTLRDAQTRLNSMGYDAGPADGVAGRRTAAALRAF
                     QRDHGLTASGRLDEATRAALTTLR"
     gene            complement(292..1719)
                     /locus_tag="PKV30_11285"
     CDS             complement(292..1719)
                     /locus_tag="PKV30_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRL30826.1"
                     /translation="MNAPTQLAHVTPEIKPRPAPPALIEALKSRFGAQFSEAMAVREQ
                     HGRDESAFTTVPPPAGVVFAQSTADVQDAIKLCAQHKVPVIPYGVGSSLEGHLLAVQG
                     GISLDVSRMNQVLAVNAEDLTVTVQPGVTRKQLNDEIKSTGLFFPIDPGADASIGGMA
                     ATRASGTNAVRYGTMRENVLALEVVTAQGEAIRTGTRAKKSSAGYDLTRLFVGSEGTL
                     GVITEVTLRIYPLPEAVSAAICSFPTIADAVTTVIQTIQIGVPIARVELIDGNSVRAV
                     NQYAKLSLREEPMLLMEFHGSPAGVKEQAETVQEIAREHRGADFEWADTPEERTRLWT
                     ARHNAYFAAVQSRPGCRCITTDTCVPISRLADALLDTVAEADESGIPYFLVGHVGDGN
                     FHVGYLIDPDDDDERARAEALNHRVVQRAIALQGTCTGEHGVGLHKMGFLIDEAGAGA
                     VAMMRTIKQALDPDNIMNPGKVFAV"
     gene            1944..2783
                     /gene="aroE"
                     /locus_tag="PKV30_11290"
     CDS             1944..2783
                     /gene="aroE"
                     /locus_tag="PKV30_11290"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRL30827.1"
                     /translation="MPDRYAVIGHPIAHSKSPLIHGLFAQATQQDMTYEAIDGGADDG
                     GFMRALAAFRAAGGLGLNVTLPFKLQALAAADQASDDARLAGAANALRFEGERIEARN
                     FDGIGLVRDIEVNLGLPLKGQRVLLLGAGGATRGALAPIARAGASRIVIANRTAGKAA
                     ALAQEMAPHLPGTVVEGGGFGDLAPGDAFDIVINATAASLAGEAPAVPASAFAAGALA
                     YDMVYGKGLTPFLKLAQAAGAARVADGVGMLVEQAAEAFAWWRGVRPDTRAVLQRLTV
                     PLA"
     gene            2799..3248
                     /gene="aroQ"
                     /locus_tag="PKV30_11295"
     CDS             2799..3248
                     /gene="aroQ"
                     /locus_tag="PKV30_11295"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRL30828.1"
                     /translation="MNLLVLHGINLNMFGQRDPAQYGTATLADIDAALHQLAGELGAA
                     VECFQTNHEGAMCERIHQAHRDGVAAVVINAGAWTHTSVAIRDALAILKCPIVEVHMS
                     NIHAREPFRQHSYIAPIARGQICGFGVDSYLLGLRAAASAAQTAINK"
     gene            3258..>3333
                     /locus_tag="PKV30_11300"
     CDS             3258..>3333
                     /locus_tag="PKV30_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HRL30829.1"
                     /translation="MGKRLTQIATRTGDDGTTGLGDNTR"
BASE COUNT          483 a         1197 c         1131 g          522 t
ORIGIN      
        1 agccggcgcc cgtcgtcgcc cccgcgcgcc tcacgctgcg cgacgcgcag acgcggctga
       61 acagcatggg ctacgacgcc ggcccggccg acggcgtggc cgggcgccgc actgcggcgg
      121 cgctgcgcgc cttccagcgc gatcatggcc tgaccgccag cggccggctg gacgaggcca
      181 cgcgcgcagc gctcaccacg ttacgctgat ctgcttcaat aacaggagct gctggcgccc
      241 gccagtattg ccctgaaggc cgatttggct tgaaatcgac cgggcggtgc cctacaccgc
      301 aaacaccttc cccgggttca tgatgttgtc cgggtccagc gcctgcttga tggtgcgcat
      361 catcgccacc gcgccggcgc cggcttcgtc gatcagaaag cccatcttgt gcagccccac
      421 gccgtgctcg ccagtgcagg tgccctgcag cgcgatggcg cgctgcacca cgcggtggtt
      481 cagcgcctcg gcgcgggcgc gctcgtcgtc gtcgtcgggg tcgatcaggt agccgacgtg
      541 gaagttgcca tcgcccacgt gcccgaccag gaagtacgga atgccgctct cgtccgcctc
      601 ggccaccgtg tccagcagcg catcggccag gcggctgatg ggcacgcagg tgtcggtggt
      661 gatgcagcgg cagccagggc ggctttgcac ggcggcaaag taggcgttgt gccgcgccgt
      721 ccacaggcgg gtgcgctcct cgggcgtgtc ggcccattcg aagtcggcgc cgcggtgctc
      781 gcgggcgatc tcctgcaccg tctcggcctg ctccttcacg ccggccggcg agccgtggaa
      841 ctccatcagc agcatcggct cctcgcgcag gctcagcttg gcgtactggt tcacggcgcg
      901 cacgctgttg ccgtcgatca gttccacgcg cgcgatcggc acgccgatct ggatggtctg
      961 gatcaccgtg gtcaccgcgt cggcgatggt cgggaacgag cagatcgccg ccgacaccgc
     1021 ctcgggcagc gggtagatgc gcagcgtgac ctcggtgatc acgcccagcg tgccctcgct
     1081 gcccacgaac aggcgcgtca ggtcgtagcc ggcgctgctc ttcttggcac gcgtgccggt
     1141 gcggatggcc tcgccctgcg ccgtcaccac ttccagcgcc agcacgttct cgcgcatggt
     1201 gccgtagcgc acggcgttgg tgccgctggc acgcgtggcc gccatgccgc cgatgctggc
     1261 atcggcaccg gggtcaatcg ggaagaatag gccggtgctc ttgatctcgt cgttgagctg
     1321 cttgcgcgtc acgcccggct gcaccgtcac tgtcaggtcc tcagcgttca cggccagcac
     1381 ctggttcatg cggctcacgt ccaggctgat gccgccctgc accgccagca ggtggccctc
     1441 cagcgacgag cccacgccgt acggaatcac tggcaccttg tgttgcgcgc acagcttgat
     1501 ggcgtcctgc acgtcggccg tgctctgggc gaacaccacg cccgccggcg gcggcaccgt
     1561 ggtgaaggcc gactcgtcgc gcccgtgctg ctcgcgcacc gccatggcct cggaaaactg
     1621 cgcgccgaag cgcgatttca gcgcctcgat cagcgccggc ggtgccgggc gcggcttgat
     1681 ctcgggcgtg acgtgggcaa gctgggtggg ggcgttcatg gcagatgtct ccaggcgggc
     1741 gcggccgcgg caatcggggg atcggccagt ttaagccgat cgggcgcagc gcgctcggtg
     1801 cgggcttggg tgtgagtact cctgattgca tagctgttag cgcttgctgg acgggcgctg
     1861 aaggccaatt tggctcataa gccgtgcccc tgccaccctg cgctacagtg acagccccgt
     1921 ctgccatcca ctccccgccc gccatgcccg accgctacgc cgtcatcggc caccccatcg
     1981 cccacagcaa gtcgccgctg attcacgggc tgttcgcgca ggccacgcag caggacatga
     2041 catacgaagc cattgacggc ggtgcggatg atggcggctt catgcgtgcg ctggccgcgt
     2101 tccgcgccgc tggcggcttg gggctcaacg tcacgctgcc cttcaagctg caagcgctgg
     2161 ccgccgccga ccaggccagc gacgacgcac gcctggccgg cgccgccaac gcgctgcgct
     2221 ttgaaggcga acgcatcgag gcgcgcaact tcgacggcat cgggctggtg cgcgacatcg
     2281 aagtcaatct gggtctgccc ttgaaaggcc agcgcgtgct gctgctgggc gccggcggcg
     2341 ccacgcgcgg tgcgctggca cccatcgcgc gggccggcgc gtcgcgcatc gtcatcgcca
     2401 accgcacggc gggcaaggct gcggcgctgg cgcaggaaat ggcgccgcat ctgccgggca
     2461 ccgtggtcga aggcggcggc ttcggcgatc tggcgccggg cgacgccttc gacatcgtca
     2521 tcaacgccac cgccgcgtcc ctcgccggtg aagcgcccgc cgtgcccgcc agcgcgttcg
     2581 ctgccggcgc gctggcctac gacatggtct acggcaaggg cctcacgccg ttcctgaaac
     2641 tggcgcaagc agccggcgcc gctcgcgtgg ccgacggcgt cggcatgctg gtcgagcagg
     2701 cggccgaagc cttcgcctgg tggcgcggcg tgcggcccga tacgcgcgcg gtgctgcagc
     2761 ggctgaccgt gccgctggcg tgattcacaa tggctgccat gaacctcctc gtcctgcacg
     2821 gcatcaacct gaacatgttc ggccagcgcg acccggcgca gtacggcacg gccacgctgg
     2881 ctgacatcga cgccgcgctg caccagctgg cgggcgagct gggcgcggcg gtggaatgct
     2941 ttcagaccaa ccacgaaggc gcgatgtgcg agcgcatcca ccaggcgcac cgcgatggcg
     3001 tggcggcggt ggtcatcaat gccggcgcgt ggacgcacac cagcgtcgcc atccgcgacg
     3061 cgctggccat cctgaagtgc cccatcgtcg aggtccacat gagcaacatc cacgcccgcg
     3121 agccgttccg ccagcactcc tacatcgcgc caatagcgcg cgggcagatc tgcggcttcg
     3181 gcgtcgattc ctacctcttg gggcttcgag ccgccgccag tgctgcgcaa acagctatca
     3241 ataagtgagt taacgacatg ggcaagcgcc tcacgcagat cgccacccgc accggcgacg
     3301 acggcaccac cggcctgggc gacaacacgc gcg
//
LOCUS       DAOMVO010000274         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8373_length_3332_cov_14.362832, whole genome shotgun sequence.
ACCESSION   DAOMVO010000274 DAOMVO010000000
VERSION     DAOMVO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8373_length_3332_cov_14.362832"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="PKV30_11305"
     CDS             complement(<1..1682)
                     /locus_tag="PKV30_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30830.1"
                     /translation="MSGPITPHKDPRSDWRPDSNPIYDKTNDAIESSTKPVVEAVHKQ
                     TEQGGWQGKIDRLDSKGDTARSRTMLDGELDIGVLNLIPGGAAAKAALKATKVLDINL
                     DLAVKGQSAHLIDVTRDSNAADSTYTVRYTKMSQGGLGAELGLPLGGTVMGSKGVMGL
                     GGTLNGFVRADGYVGTANTVEFRFNTKEEAARAAASMEKLAAADMVDDGVQAALLTGS
                     APGQPQAPLINTLGQTYEGSKNNFANPMSEYGEVNPGLRRAAGVSDEEMSFLADHITA
                     QEVSVMGGGRGAVEGRLTADGKFNLPSGSKVKLPGLELKTGSAIVPNVYRNTSYTRRV
                     EYPTDVSKGRIVDSFNSYDQLSAKWRDVKNVGLNFPGQNGSGGATVTGQQRADDNYEL
                     AIMNSSVSFQQDIPAGTRVDLGYLAGKPPAFDKPAAVDFSLMTPTGRDGLGLARTLNP
                     LDLSGRGQAPDGQQRVDMNRLHMRYQVDGAAGLAMAGSMPVTGSGAGLLGQGLRQGDF
                     IEYQRDTVKRDGNNTTQTLRAGLAGVVAVEGSLTKYGGVDDYDTAAAPVPPPP"
     gene            complement(1880..2467)
                     /locus_tag="PKV30_11310"
     CDS             complement(1880..2467)
                     /locus_tag="PKV30_11310"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRL30831.1"
                     /translation="MSQPLVQADFAGDAADVARRLIGATLLVDGVGGVIVETEAYDQS
                     EPAAHTHRGPTPRNAAMFGPPGHAYVYFNYGIHWCLNFVCREAGHGAGVLIRALQPTH
                     GVDVMRTRRGMDDERLLCAGPGRVGQALGIGTAHYGLPLDAAPFTVRAAPADAAPVQV
                     LTGPRIGISRAVDVPWRFGLAGSRHLSRGFRPPRT"
     gene            2548..>3332
                     /locus_tag="PKV30_11315"
     CDS             2548..>3332
                     /locus_tag="PKV30_11315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HRL30832.1"
                     /translation="MFKALLLTKTDDAPLSAAVTDLDDDRLPAGDVLVQVASSTLNYK
                     DALAITGKAPVVRSYPMVPGIDLAGMVVESSDQRYRPGDPVLLNGWGVGESHWGGLAQ
                     RARVKADWLLPLPGGMTPSQAMAIGTAGYTAMLCVMALQQNGVATDHGPVLVTGANGG
                     VGSIAIALLSRLGFTVHAATGRPHEAEHLKALGASEVIDRSTLNAPGKPLQKERWAAA
                     IDSVGSHTLVNVCAGIKYGGVVAACGLAQGMDFPGTVAPFILRG"
BASE COUNT          479 a         1095 c         1174 g          584 t
ORIGIN      
        1 ggtggcggcg gcacgggagc ggcggcggtg tcgtagtcgt ccacgccgcc gtacttggtc
       61 aggctgccct cgacggccac cacgcccgcc aagccggcgc gcagggtctg ggtcgtgttg
      121 ttgccgtcgc gcttgacggt gtcgcgctga tattcgatga agtcgccctg gcgcagaccc
      181 tggcccagca ggccggcgcc actgcctgtg accggcatgg agccggccat ggccagcccg
      241 gcggcgccat cgacctgata gcgcatgtgc aggcggttca tgtccacgcg ctgctggcca
      301 tccggcgcct ggccgcggcc tgagaggtcg agcgggttga gggtgcgcgc caggccgagc
      361 ccgtccctgc ccgtgggggt catgagtgag aaatccacgg cggccggttt gtcgaacgca
      421 ggcggcttgc cggcaaggta gcccaggtcg acacgcgtgc cggccggaat gtcctgttgg
      481 aaagagaccg aactgttcat gattgccagt tcgtagttgt cgtcggcacg ctgctggccg
      541 gtgacggtcg cgccgccgga gccgttctgg ccggggaaat tcagaccgac gttcttcacg
      601 tcgcgccatt tggcggacaa ctggtcgtag gagttgaagc tgtcgacgat gcggcccttg
      661 gacacgtcgg tgggatattc gacgcggcgc gtgtaagacg tgttgcggta cacgttgggc
      721 acgatggcgc tgccggtctt caattcgagc ccgggcagct tgaccttgct gccactgggc
      781 aggttgaact tgccgtcggc cgtgagccgg ccttcgaccg cgccgcggcc gccgcccatc
      841 accgacacct cttgcgccgt gatgtgatcg gccaggaagg acatttcctc gtcgctgacg
      901 ccagccgcgc ggcgcaggcc ggggttgacc tcgccgtact cgctcatcgg gttggcgaag
      961 ttgttcttgc tgccctcgta agtttgcccc agggtattga tcagcggcgc ctgcggctgg
     1021 cccggcgcgg aacccgtgag caacgccgcc tgcacgccgt cgtccaccat gtcggcggcg
     1081 gcgagttttt ccatgctggc agcggcgcgc gcggcttctt ccttggtgtt gaagcggaat
     1141 tccacggtgt tggcggtgcc gacgtagcca tcggcgcgga cgaagccgtt cagcgtgccg
     1201 cccaggccca tcacgccctt ggagcccatg accgtgccac ccagcggcag gcccagctcg
     1261 gcccccaggc cgccctggct catcttggtg tagcgcacgg tgtaggtgct gtcggccgca
     1321 ttgctgtcgc gcgtgacgtc gatcaagtgc gccgattggc ctttgaccgc cagatccagg
     1381 ttgatgtcca gcaccttggt cgccttgagc gcggccttgg ctgccgcgcc gccgggaatc
     1441 aggttgagca cgccgatgtc cagttcgccg tccagcatgg tgcgtgaacg ggcagtgtcg
     1501 cccttgctgt cgagccggtc gatcttgcct tgccagccgc cctgctcggt ctgcttgtgc
     1561 acggcctcga ccaccggctt ggtgctggac tcgatcgcgt cgttggtctt gtcgtagatc
     1621 gggttggagt cgggccgcca gtcggagcgt gggtctttgt ggggggtgat cgggccggac
     1681 atgacgctcc tgatgaatgt gtcggtgtga tgacagcatt ggagcagcgc tgagtggcgc
     1741 gcaatagctg ggatcgtttc gtaggtaggc ttaccagtgg ggcagcgtca tcacgctgtt
     1801 ttgctatttt tttgatatct aacaatgtcc cccatcaagc gctggcggcc atttttgcga
     1861 tagagcggag caaacgccgc tacgtgcgcg gcggccgaaa gccgcggctc aggtggcgcg
     1921 agccggccag gccaaagcgc cagggcacat ccaccgcgcg gctgatgccg atgcgcgggc
     1981 cggtcaacac ttgcacgggc gccgcgtcgg cgggcgctgc ccgcacggtg aacggcgccg
     2041 catccagcgg caggccgtag tgcgccgtgc cgatgcccag cgcctggccg acgcgccccg
     2101 ggccggcgca cagcaggcgc tcgtcgtcca tgccgcgacg tgtgcgcatc acgtccacgc
     2161 catgcgtcgg ctgcagcgcg cggatcagca cgccggcgcc gtggccggct tcgcggcaga
     2221 cgaagttcag gcaccagtgg atgccgtagt tgaagtacac gtaggcgtgg ccgggtgggc
     2281 cgaacatggc ggcgttgcgc ggcgtcgggc cgcggtgcgt gtgcgcggcc ggctcgctct
     2341 ggtcgtaggc ctcggtctcc acgatcacgc cgcccacgcc atcgaccagc agcgtggcgc
     2401 cgatcagccg gcgcgcgacg tcggcggcgt cgccggcgaa atcggcttgg accagaggtt
     2461 gggacatgac gcctgcaagt ctatgcccac gtcgcagtgg cttatagtgc ctcgtgaccc
     2521 gcccgcactt ttgaaaagga caccgccatg ttcaaggccc tgctgctgac caagaccgac
     2581 gacgccccgc tttccgccgc cgtcaccgac cttgacgacg atcgcctgcc cgccggcgat
     2641 gtgctggtgc aggtggcgtc ttccacgctc aactacaagg acgcgctggc catcaccggc
     2701 aaggcgccgg tggtgcgcag ctacccgatg gtgccgggca tcgacctcgc cggcatggtg
     2761 gtcgaatcca gcgaccagcg ctatcggccg ggcgacccgg tgctgctgaa cggctggggc
     2821 gtcggcgaat cgcattgggg cgggctggcg caaagagcgc gcgtgaaggc cgactggcta
     2881 ctgccgctgc caggcggcat gacgccgagc caggccatgg ccatcggcac cgccggctac
     2941 accgccatgc tgtgcgtgat ggcgttgcag caaaacggcg tcgccaccga ccacggcccg
     3001 gtgctggtga ccggcgccaa cggcggcgtc ggctcgatcg ccattgcgct gctgtcgcgc
     3061 ctgggtttca ccgtgcacgc agccaccggc cgcccgcacg aagccgagca tttgaaggcg
     3121 ctcggcgcca gcgaggtgat cgaccgatcg accctgaacg cgcccggcaa accgctgcaa
     3181 aaggagcgct gggccgccgc catcgacagt gtgggcagcc acacgctggt taacgtttgc
     3241 gcgggcatca aatacggcgg cgtggtggcg gcctgcgggc tggcgcaggg catggacttt
     3301 cccggcacgg tggcgccgtt catcctgcgc gg
//
LOCUS       DAOMVO010000275         3318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8415_length_3318_cov_11.979773, whole genome shotgun sequence.
ACCESSION   DAOMVO010000275 DAOMVO010000000
VERSION     DAOMVO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8415_length_3318_cov_11.979773"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKV30_11320"
     CDS             <1..404
                     /locus_tag="PKV30_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL30833.1"
                     /translation="VVERDSPSLGVHLTLDLGGQAKFGPDVQWVDSPHDLEVDAARGN
                     AFYAEVRRYWPELRDGALQPGYAGIRPKISAPGEPAADFVIQGPREHGVPGLVNLFGI
                     ESPGLTSCLALGAATVEALNGDSVAMQACEP"
     gene            427..630
                     /locus_tag="PKV30_11325"
     CDS             427..630
                     /locus_tag="PKV30_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRL30834.1"
                     /translation="MAANVSLDPDLLEEAFRLSGEPTKKAAVTRALQEFIARREQRRV
                     ADLFGKLTWDEAFDHKAERSRQP"
     gene            702..1034
                     /locus_tag="PKV30_11330"
     CDS             702..1034
                     /locus_tag="PKV30_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL30835.1"
                     /translation="MALHTALLGAYQVFTTGLVLQELLQGFAGPKAGEQIIERFATLA
                     FLQPDREDHIEAAQVRNACRRKGVQVGTIDALLIQLCRRHDLVLLSTDKNFENARQHV
                     PFGVWQSA"
     gene            complement(1045..2106)
                     /locus_tag="PKV30_11335"
     CDS             complement(1045..2106)
                     /locus_tag="PKV30_11335"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRL30836.1"
                     /translation="MALLPYGLARPLLFGMDPETAHELTLDMLARGQGTPLQCAWAAQ
                     RVSDPVTLAGLRFPNRVGLAAGLDKNARAIDALAAMGFGFVEVGTVTPLAQPGNPKPR
                     MFRLPAKNALINRLGFNNEGLDAFVRNVKNSRFNSASRLPDQRERMILGLNIGKNAAT
                     PIERATDDYLIGLAGVYPYADYVTVNISSPNTKNLRELQSEAALDALLSALKSRQGEL
                     AAEHGRQVPLFLKIAPDLDESQVQAIADALRRHGIEGVIATNTTLSREAVQGLPHADE
                     AGGLSGAPVLAASNRVIRLLRATLGAGFPIIGVGGVLSGADAIGKLDAGADVVQVYTG
                     LIYKGPGLVNEVARALAAR"
     gene            2154..2828
                     /gene="rpiA"
                     /locus_tag="PKV30_11340"
     CDS             2154..2828
                     /gene="rpiA"
                     /locus_tag="PKV30_11340"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRL30837.1"
                     /translation="MTQDELKAMVGQAALDHVVPGEIVGVGTGSTVNHFIDALGQVKE
                     RIVGAVSSSVASTQRLQALGIKVFDCNEVGEIAVYIDGADEIDGSGHMIKGGGAALTR
                     EKIVAAQAKRFVCIADGSKLVEVLGRFPLPVEVIPMAAQRITRQFAAMRAEATLRMRD
                     GAPLVTDNGQHILDVRGLAIRDPLGFESEVNQWPGVVTVGVFAHQRAKVCLLGTPTGV
                     RTLSFD"
     gene            2834..>3318
                     /locus_tag="PKV30_11345"
     CDS             2834..>3318
                     /locus_tag="PKV30_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30838.1"
                     /translation="MPLIDERRLLSAALRAMLAVAALAALLLAWRYASGPARPDGPPS
                     VRVIKLLPGTFVWADAPADARYLPAGLRPQDAARLKLLVLRDEDGIVRAFWLPQQDGQ
                     PGVPTDASPAGGGIACRDFAPDFHAADIACRQAAPGFDFALRHRWSLSGKALSAGSPD
                     L"
BASE COUNT          499 a         1101 c         1173 g          545 t
ORIGIN      
        1 ccgtggtcga gcgcgattcg cccagtctgg gcgtgcatct cacgctcgac ctgggtggcc
       61 aggccaagtt cgggcccgac gtgcagtggg tcgattcacc ccatgacttg gaggtcgatg
      121 cggcgcgggg caatgccttt tacgccgagg tgcggcgcta ctggcccgaa ttgcgcgatg
      181 gcgccttgca gccgggctat gcgggtattc ggcccaagat cagcgcgccc ggcgagccag
      241 cggccgattt cgtcatccag gggccgcgcg agcatggcgt gccggggctg gtcaatctgt
      301 ttggcattga atcgccgggg ttgacgagtt gcttggcgct gggagcggcc acggtggagg
      361 cactgaatgg cgactctgtt gccatgcagg cgtgcgagcc ctaaatttct gtgtaaggag
      421 ttacacatgg ccgccaatgt ctcacttgat ccggatctgc tggaggaagc tttccgcctc
      481 agcggcgagc cgaccaagaa ggctgccgtg acgcgcgcct tgcaggaatt catcgcccgg
      541 cgcgagcagc ggcgcgtggc cgatttgttc ggcaagctga cgtgggatga ggcgttcgac
      601 cacaaggccg agcgatcacg ccagccgtga gcctgctggt cgataccagc gcctggtcgc
      661 tggcattgcg gcgcgagggc gcggaggatg ccccctaagt gatggcactg cacaccgcct
      721 tgctgggcgc ctaccaggtc ttcaccaccg ggctggtgct gcaggaattg ctgcaaggtt
      781 tcgccgggcc gaaggcgggc gaacaaatca tcgaacgctt tgccacgctg gcttttttgc
      841 agcccgaccg cgaagaccac atcgaggcgg cgcaggtgcg caacgcctgc cgccgcaaag
      901 gcgtgcaggt cggcacgatc gacgcattgc tgatccagtt gtgccgccgg cacgatctgg
      961 tgctgctgag cacggacaaa aacttcgaga acgcgcggca gcatgtaccg ttcggcgttt
     1021 ggcagagcgc gtagcgagcg gcttctagcg cgccgccagc gcccgcgcca cctcgttcac
     1081 cagccctggc cccttgtaga tcaggccggt gtagacctgt accacgtccg ccccggcgtc
     1141 gagcttgccg atggcgtcgg cgccgctcag cacgccgccg acgccgatga tgggaaagcc
     1201 cgcgcccagc gtcgctcgca gcagccggat gacgcggttg ctggccgcca gcaccggcgc
     1261 gcccgacagg ccgccggcct catcggcgtg cggcaggccc tgcaccgctt cgcgcgacaa
     1321 ggtggtgttg gtggcgatga cgccctcgat gccgtgccgg cgcagtgcat cggcaatggc
     1381 ctgcacctgg ctttcatcca ggtcgggcgc gatcttgagg aacagcggta cctgccggcc
     1441 gtgctcggcg gccagctcgc cctggcgcga cttcagcgcc gacagcaggg catccagcgc
     1501 ggcctcgctt tgcagctcgc gcaggttctt ggtgttgggg ctggagatgt tcaccgtcac
     1561 gtagtcggcg taggggtaca cgccggccag gccgatcaga tagtcgtcgg tggcgcgctc
     1621 gatcggcgtg gccgcgttct tgccgatgtt aagacccaga atcatgcgct cacgctgatc
     1681 tggcaaccgc gaggcgctat taaagcgaga gtttttgacg ttgcggacga aggcgtccag
     1741 cccctcgttg ttgaagccca ggcggttgat cagcgcgttc ttggccggca ggcggaacat
     1801 gcgtggcttg gggttgccgg gctgcgccag cggcgtgacg gtgcccacct cgacaaagcc
     1861 gaagcccatg gccgccaagg cgtcgatggc gcgcgcgttc ttgtccagcc ccgcggccag
     1921 gccgacgcgg ttgggaaagc gcaggcccgc cagcgtcacc ggatcgctca cgcgctgcgc
     1981 cgcccaggcg cactgcagcg gcgtgccctg gccgcgcgcc agcatgtcga gcgtgagttc
     2041 gtgcgccgtt tcagggtcca tgccgaacag cagggggcgc gccagaccgt aggggagcag
     2101 ggccatcgga taattcttgt ttcgaccgtt gatgtgaaga ggaattgtcg caaatgaccc
     2161 aggacgaact gaaggctatg gtgggccagg ccgcgctgga ccatgtggtg ccgggcgaga
     2221 tcgtgggcgt gggcacgggg tcgacggtca atcatttcat cgacgcgctg ggtcaggtca
     2281 aggagcgcat cgtcggcgcc gtgtcgtcgt cggtggcctc cacgcagcgg ctgcaggcgc
     2341 tcggcatcaa ggtgttcgat tgcaacgaag tgggcgagat cgcggtctac atcgacggcg
     2401 ccgacgagat cgacggcagc ggccacatga tcaagggcgg cggcgcggcg ctgacgcgcg
     2461 agaagatcgt cgccgcgcag gcaaaacgct tcgtctgcat cgcagacggg tccaagctgg
     2521 tggaggtgct ggggcgcttt ccgctgccgg tcgaggtgat tcccatggcc gcgcagcgca
     2581 tcacgcgcca gttcgccgcc atgcgggccg aggccacgct gcgtatgcgt gacggcgcgc
     2641 cgctggtcac cgacaacggc cagcacatcc tggacgtgcg cggcctcgcc atccgcgacc
     2701 cgctgggctt cgagagcgaa gtcaaccagt ggcccggcgt ggtgacggtg ggcgtgttcg
     2761 cgcaccagcg cgcgaaggtc tgcctgctgg gcacgcccac gggcgtgcgc acgctgagct
     2821 tcgactgatc gccgtgccgc tgatcgacga gcggcgcctg ctcagcgccg cgctgcgcgc
     2881 catgctggcc gtggcggcac tggccgctct gctgctggcc tggcgttacg ccagcgggcc
     2941 ggccaggccc gacgggccgc ccagcgtgcg cgtgatcaag ctgctgccag gcacctttgt
     3001 gtgggccgac gcgcccgccg acgcgcgcta cctgcccgcc ggcctgcgcc cgcaggacgc
     3061 cgcgcgcctg aagctgctgg tgctgcgcga tgaggacggc atcgtgcgcg ccttctggct
     3121 gccccagcag gacggccagc ccggagtgcc caccgacgcc tcaccggccg gcggcggcat
     3181 tgcgtgccgc gacttcgcgc ccgacttcca cgccgccgac atcgcctgcc gccaggccgc
     3241 gcccggcttc gacttcgcgc tgcgccaccg ctggtcactg tccggcaagg cgctcagcgc
     3301 cggctcgccc gacctgca
//
LOCUS       DAOMVO010000276         3316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8421_length_3316_cov_17.910150, whole genome shotgun sequence.
ACCESSION   DAOMVO010000276 DAOMVO010000000
VERSION     DAOMVO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8421_length_3316_cov_17.910150"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..1020)
                     /locus_tag="PKV30_11350"
     CDS             complement(331..1020)
                     /locus_tag="PKV30_11350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRL30839.1"
                     /translation="MGQFIDLTAADGQKIPAYVAEPAGQPKGAVVVLQEIFGVNAHIR
                     EVADGYAKDGYLAVAPSTFQRVKPGVDLGYTDADMQEGFGYKNAVEALPAPGVMQDIQ
                     AAIDYAAQKSGGKVGIVGYCWGGLLTWRAAAMLNGLSAAVPYYGGGMTQPAEIARQPK
                     VPVLAHLSDRDQWVPLDGVEAFKKAHPEVEVHLYPANHGFNCDHRAAWDAESAQLARE
                     RTLVFFAKHLG"
     gene            complement(1234..1836)
                     /locus_tag="PKV30_11355"
     CDS             complement(1234..1836)
                     /locus_tag="PKV30_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRL30840.1"
                     /translation="MTRTVLYYFAPQSPWSYLGHARLVDLLARSRREVAVLPVDYGRI
                     FPQSGGLPLAKRAPQRQAYRLVELKRFAEHLGLALNPQPRYFPVDATPSARLITAVAQ
                     QDGMAAAMALAGAVLAACWREERDIARDATLAELLEECDLPAERLAESRGADTQARYD
                     AHTQAAMDAGVFGAPTYVIDGELFWGQDRLDFVARKLGIV"
     gene            1890..2642
                     /locus_tag="PKV30_11360"
     CDS             1890..2642
                     /locus_tag="PKV30_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL30841.1"
                     /translation="MAPDKVLLVTGGGRGIGAATARLAAQGGWAVAVNYAVNSLAADE
                     VVRAIRAGGGTAISVQADVADEAQVLKMFAHVDSKLGRLTGLVNNAGVVDRAQPVAEQ
                     SVARWRRMFDINVIGSMICAREAVRRMGTAHGGAGGSIVNLSSAAARLGSPNEYVDYA
                     AAKAAIDAFTIGLAKEVAPQGIRVNAVRPGLIETEIHASGGRPNRVEELKAGVPMGRG
                     GTADEVAQLIVWLLSDAASYTTMSLIDVSGAR"
     gene            2649..3143
                     /locus_tag="PKV30_11365"
     CDS             2649..3143
                     /locus_tag="PKV30_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRL30842.1"
                     /translation="MSDHPQQHIKYFWEDLPVGTTLDIGSTTVDRDEVIAFASKYDPQ
                     PFHLDEAAAAKSMFGKLSASGWHTCAMAMGLMVRGFLHESSSLGSPGLEKVSWLTPVY
                     PGDTLTLKQTILESRPMKSRPDVGLTRTLWEMFNQHGEQVLMMDGYGMFRRRTPAPAD
                     SASK"
BASE COUNT          564 a         1090 c         1130 g          532 t
ORIGIN      
        1 cgatgggatg tagtttgcgt ctgcgagcca gtttaaaaaa ccacctgaaa gtgaatgaac
       61 catttccgat ttcgaaacaa tcgggatgca ggagggttgt gcgaaggcgc agcgacgcgc
      121 ccgcgcggtg ggcgcgtcgt cagcttgtcc agcgttctgc gctgggcgtt ttagggcgtg
      181 gcggcgcatc ggcgccgcgc cataccgcag cgggcgccac tcggcgcggc agcgcggcta
      241 gatgctgaaa agcgacgcca gcgcccgccc gatgtgcgcc agcagctaca tgatcgatag
      301 cgccagccct gcgccgccct gcccgccagc tcagcccagg tgcttggcaa agaagaccag
      361 cgtgcgctcg cgcgccagct gggccgattc ggcgtcccac gccgcccggt ggtcgcagtt
      421 gaagccgtgg ttggccgggt acaggtgcac ctcgacctcg gggtgcgcct ttttgaacgc
      481 ttccacgccg tccagcggca cccactggtc ccggtcggac aggtgcgcca gcacgggcac
      541 cttgggctgg cgcgcaatct cggccggctg cgtcatgccg ccgccgtagt aaggcacggc
      601 ggccgacagg ccgttcagca tggccgccgc gcgccaggtc agcagcccgc cccagcagta
      661 gccgacgatg ccgaccttgc cgccgctttt ttgcgcggcg tagtcgatgg cggcctggat
      721 gtcctgcatc acgccgggcg cgggcagcgc ctcgacggcg ttcttgtagc caaagccttc
      781 ctgcatgtcg gcgtcggtgt agcccagatc cacgccgggc ttgacccgct ggaaggtgct
      841 gggcgccacg gccaggtagc cgtccttggc gtagccgtcg gccacttcgc ggatgtgggc
      901 gttgacgccg aaaatctcct gcagcaccac gacggcgccc ttgggctggc cggccggttc
      961 ggccacgtag gcggggattt tctggccgtc ggcggcggtg aggtcgatga attggcccat
     1021 ggtgatttgc tcctgattaa atagctgtct gcgcttgtct ggcaagcgcc agtgcccaaa
     1081 atgaacaatg atcggcgcgg cgcgccatga ccttgcttgc gctcgatgac ggtgcccttc
     1141 gggcagcatg acttctgtca tgcgcccaga gggcgcgtca tcgaaggccg caggccgagg
     1201 tcatgcgctc gccagagcgc ggtcatcgat cgctcaaaca atgcccaact tgcgggcgac
     1261 aaaatccagc cggtcctgcc cccagaacag ctcgccgtcg atcacgtagg tcggcgcgcc
     1321 gaacacgcca gcgtccatgg cggcctgggt gtgggcatcg tagcgcgcct gtgtgtcggc
     1381 gccgcgcgac tcggccaggc gctcggcggg cagatcgcat tcttcgagca gttcggccag
     1441 cgtggcgtcg cgggcaatgt cgcgctcttc gcgccagcag gcggccagca cggcgccggc
     1501 cagcgccatc gcggcggcca tgccgtcttg ctgcgccacg gcggtgatca ggcgcgccga
     1561 cggcgtggcg tcgaccggaa agtagcgcgg ctgcggattc agcgccaacc ccaggtgctc
     1621 ggcaaagcgc ttcagctcga ccagccggta ggcctgccgc tgcggcgcgc gcttggccag
     1681 cggcagcccg cccgattgcg gaaagatgcg cccgtagtcc accggcagca ccgccacctc
     1741 gcgccggctg cgcgccagca aatcgaccag gcgcgcatgg ccaagatagc tccacgggct
     1801 ttgcggcgcg aagtaataca gaacggtgcg ggtcatgcaa gtctcctgaa ataatgatct
     1861 tcgagcgtga tttttacagg agatgcggcg tggcaccaga caaggttttg ctggtcaccg
     1921 gcggcggccg gggcattggc gccgccaccg cgcggctggc ggcgcagggc ggttgggcgg
     1981 tggcggtgaa ctacgccgtc aattcgctcg ccgccgacga ggtggtgcgc gccatccgcg
     2041 ccggcggcgg caccgccatc agcgtgcagg ccgacgtggc tgatgaagcc caggtgctca
     2101 agatgttcgc gcacgtcgat tcaaagctgg ggcgcctcac gggcctggtc aacaacgccg
     2161 gcgtggtgga tcgggcgcaa ccggtggccg agcaaagcgt ggcgcgctgg cgccgcatgt
     2221 tcgacatcaa cgtcatcggc tcgatgattt gcgcgcgcga ggcagtgcgg cgcatgggca
     2281 cggcgcacgg cggcgcgggc ggcagcatcg tcaacctttc cagcgcggcg gcgcggctcg
     2341 gctcaccgaa cgaatacgtc gactatgcgg ccgccaaggc cgccatcgac gccttcacca
     2401 tcggcctggc gaaagaagtg gcgccgcagg gcattcgcgt caacgccgtg cggccggggc
     2461 tgatcgagac cgaaattcac gccagcggcg gccggccgaa ccgcgtcgaa gaactgaaag
     2521 ccggcgtgcc catgggccgc ggcggcacgg cggacgaagt cgcgcagttg atcgtctggc
     2581 tgctgtcgga tgcggcaagc tacaccacca tgtccttgat tgacgtttcc ggagcacgct
     2641 gaaaaaccat gtccgaccac ccgcaacaac acatcaagta cttctgggaa gacctgcccg
     2701 tgggcaccac gctcgacatc ggcagcacca ccgtcgaccg cgacgaggtg atcgcctttg
     2761 ccagcaagta cgaccctcag cctttccacc tggacgaagc ggcggctgcc aagtccatgt
     2821 tcggcaagct cagtgccagc ggctggcaca cctgcgcgat ggcgatgggg ctgatggtgc
     2881 gcggctttct gcacgagtcg agcagcctgg gctcgcccgg gctggagaag gtgagctggc
     2941 tgacgccggt gtaccccggc gacacgctga cgctcaagca aaccatcctc gaatcgcgcc
     3001 cgatgaaatc gcgccccgac gtcggcctga cgcgaacgct gtgggagatg ttcaaccagc
     3061 acggcgagca ggtgctgatg atggacggtt atggcatgtt caggcggcgg acgccggcgc
     3121 ctgccgattc ggcatcaaaa taagcgccag cgcttgatag acaagcgcga acagctcttt
     3181 atttgatagc aatttactta cggtccttcg cgcgcccaca ccggcggtga gccgatgctc
     3241 tcctgcagca gatcgaggaa cagccgcgtg cgcagcggca ggcggcgttc gccgtagacg
     3301 gcgtagacca tgccga
//
LOCUS       DAOMVO010000277         3309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8452_length_3309_cov_17.187769, whole genome shotgun sequence.
ACCESSION   DAOMVO010000277 DAOMVO010000000
VERSION     DAOMVO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8452_length_3309_cov_17.187769"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKV30_11370"
     CDS             <1..716
                     /locus_tag="PKV30_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRL30843.1"
                     /translation="SDGIMVARGDLAVEVGNAAVPALQKKMIRMARDADKLVITATQM
                     MESMITNPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPLETVRQMAAVCEAAEEH
                     DPVELDADFSGATFTRIDQSIAMGALFTAYHLGAKAIVALTDSGSTALWMSRHRVRIP
                     IYALTPRVSSQRKMALYRNVRPMLMDTAADRDTALKQAEAYLKRQDVVAAGDVYAITC
                     GEPMGAPGGTNMLQICRVG"
     gene            complement(738..1274)
                     /locus_tag="PKV30_11375"
     CDS             complement(738..1274)
                     /locus_tag="PKV30_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL30844.1"
                     /translation="MNTLDSSVFLWLNLGHDAPEALVWLAMACSRWLPAVLLMALLPL
                     AALGGPRGRRTVWLALLGMGLAWAAAALLKHSVAAPRPFMLGLGTEWLGHSGGNGFPS
                     SHAAVAMAFAVSMALGPWRWPWRAAAVLCGLLVGWSRIALGVHFPSDVLAAALLGTAC
                     ACGVYTLAGRVRSLAWAP"
     gene            complement(1323..2084)
                     /locus_tag="PKV30_11380"
     CDS             complement(1323..2084)
                     /locus_tag="PKV30_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL30845.1"
                     /translation="MTTSLAATNGPDRRDAPSRAAAPSTASVWLAASALGLLALVVWD
                     ASGLDLPLARWFGDASGFAHRSDWLLTKVLHDGARNASWGLTALLALMVWRPLGVLRR
                     LSRGERIGLLLGVVASVLAMSLIKRASLTSCPWDLQEFGGTATHVSHWLWGVADGGGG
                     NCFPAGHASAGFAYLAGWFWLRRVSPRAAAWWLGAALVLGFGLGAVQQVRGAHYMSHT
                     LWTAWLCWTIGGAVWWLAAGRRPRSNTNCVPTRTR"
     gene            2284..>3309
                     /gene="fba"
                     /locus_tag="PKV30_11385"
     CDS             2284..>3309
                     /gene="fba"
                     /locus_tag="PKV30_11385"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299538.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRL30846.1"
                     /translation="MPLVSMRELLDHAAANHYGIPAFNVNNLEQVQAVMAAADEVGAP
                     VILQASAGARKYAGEHFIKHLIAAAAEAYPHIPLVMHQDHGTSPKICQGAIDLGFGSV
                     MMDGSLREDGKTPADFDYNVRVTREVVDMAHKVGVTVEGELGCLGSLETGEAGEEDGV
                     GAVGKLSHDQMLTDPEEAAQFVAATQLDALAIAIGTSHGAYKFSRPPTGDVLAIGRVK
                     EIHARIPNTHLVMHGSSSVPQDLLDIINQFGGQMKQTYGVPVKEIQEAIKHGVRKINI
                     DTDIRMAMTGAVRKFQAENPDKFDMREWMKPAREAAKAICKQRYIEFGCEGQGAKIKG
                     VPLSVMAE"
BASE COUNT          615 a         1226 c         1091 g          377 t
ORIGIN      
        1 ccagcgacgg catcatggtg gcgcgcggcg acctggcggt ggaagtgggc aacgcggcgg
       61 tgccggcact gcagaaaaag atgatccgca tggcgcgcga cgccgacaag ctggtcatca
      121 ccgccacgca gatgatggaa agcatgatca ccaacccggt gcccacgcgc gctgaggtga
      181 gcgacgtggc caacgccgtg ctggacggca ccgacgccgt gatgctgtcg gccgagacgg
      241 cggccggcaa atacccgctg gaaaccgtgc gccagatggc cgccgtgtgc gaggcggccg
      301 aggagcacga cccggtggaa ctggacgccg atttcagcgg cgccaccttc acccgcatcg
      361 accagtcgat tgccatgggc gcgctgttca cggcctacca cctgggcgcc aaggccatcg
      421 tggcgctgac cgattccggc tcgaccgcgc tgtggatgag ccgccaccgc gtgcgcatcc
      481 ccatctacgc gctcacgccg cgcgtcagct cgcagcgcaa gatggcgctg taccgcaacg
      541 tgcggccgat gctgatggac accgccgccg accgcgacac ggcgctgaaa caagccgagg
      601 cctacctgaa gcgccaggac gtggtggccg ccggcgacgt ctacgccatc acctgcggcg
      661 agcccatggg cgcgcccggc ggcaccaaca tgctgcagat ctgccgcgtc ggctaaggcg
      721 cttttttcgc cgcgccgcta tggcgcccac gcgagcgagc gcacgcgccc ggccagcgtg
      781 tacaccccgc aagcgcacgc cgtgcccagc agcgctgccg ccagcacatc ggacggaaag
      841 tgcacgccca gagcgatgcg gctccaaccg accagcagcc cgcacaacac agcggccgca
      901 cgccacggcc agcgccacgg cccgagcgcc atcgacaccg cgaacgccat cgccaccgcc
      961 gcgtgcgaac tgggaaaccc gttgccgccc gaatgaccga gccattcggt gcccaggccc
     1021 agcatgaaag ggcgcggcgc cgccacactg tgcttgagca gcgcggccgc cgcccaggcc
     1081 aggcccatgc cgagcagcgc cagccacacc gtgcgccgcc cgcgcgggcc gccaagcgct
     1141 gccagcggca gcagcgccat cagcagcacg gccggcagcc accggctgca cgccatggcg
     1201 agccacacca gcgcctcggg cgcgtcgtgc cccaggttga gccacagaaa caccgatgaa
     1261 tcgagcgtat tcacacccac agcctgcagc gccagcatga cgtgccggtg aatgtcgccg
     1321 cgtcagcgcg tgcgtgtcgg cacgcagttg gtgttggacc ggggccgccg cccggccgcc
     1381 agccaccata cggcgccgcc gatggtccag cacagccagg ccgtccacag cgtgtggctc
     1441 atgtagtgcg cgccgcgcac ctgctgcacc gcgcccaggc cgaaacccag cacgagcgcg
     1501 gcgcccagcc accaggcggc cgcgcgcggc gacacgcggc gcagccagaa ccagccggcc
     1561 agatacgcaa agccggccga cgcgtgcccg gccggaaagc agttgccgcc accgccatcc
     1621 gccacgcccc acagccagtg cgacacgtgg gtcgcggtgc cgccgaattc ctgcaggtcc
     1681 cacgggcagc tggtcaggct ggcgcgcttg atcaacgaca tcgccagcac cgaggccacc
     1741 acgcccagca gcagcccgat gcgctcgccg cgcgacaggc gccgcagcac gccgagcggg
     1801 cgccagacca tcagcgccag cagcgccgtc agcccccacg acgcattgcg cgcaccatcg
     1861 tgcagcacct tggtcagcag ccagtcgctg cggtgggcaa agccgctggc atcgccgaac
     1921 cagcgcgcca gcggcaaatc gagcccgctg gcgtcccaca cgaccagcgc cagcaagccg
     1981 agcgcactgg cggccagcca cacagaggcg gtcgacggcg cggcggcgcg gctgggcgcg
     2041 tcgcgccggt cggggccgtt cgtggcggca aggctggtcg tcatgggggc gatcgtggcc
     2101 acctcatctg agcgctttct taacgccgcg ccacgtgcgc agcgcggccc cgcgccggcg
     2161 cacgggggtc accgccccag acaggccggc gcaagcacgc ttcggtaaaa ttgcacaaat
     2221 tccgggcacc ggacacaccg tgcccgacga tcgagtttct gactctcccc gaggaccctc
     2281 cccatgccac tcgtttccat gcgcgaactg ctggaccacg ccgccgccaa ccactacggc
     2341 atcccggctt tcaacgtcaa caacctcgag caggtgcagg ccgtgatggc ggcggccgac
     2401 gaagtgggcg cgccggtcat cctgcaggcc agcgccggcg cgcgcaagta cgcgggcgag
     2461 cacttcatca agcacctgat cgccgcggcc gccgaggcct acccgcacat cccgctggtg
     2521 atgcaccagg accacggcac ctcgcccaag atctgccagg gcgccatcga cctcggtttt
     2581 ggctcggtga tgatggacgg cagcctgcgc gaagacggca agacgccggc ggacttcgac
     2641 tacaacgtgc gcgtgacccg ggaagtggtc gacatggcgc acaaggtcgg cgtgacggtg
     2701 gagggcgaac tgggctgcct cggctcgctg gagaccggcg aggcgggcga ggaagatggc
     2761 gtgggcgccg tcggcaagct gagccacgac cagatgctga ccgaccccga ggaagccgcg
     2821 cagttcgtcg ccgccacgca gctcgacgcg ctggccattg ccatcggcac cagccacggc
     2881 gcctacaagt tcagccggcc gcccactggc gacgtgctgg ccatcggccg cgtgaaggaa
     2941 atccacgccc gcatccccaa cacgcacctg gtgatgcacg gctcgagcag cgtgccgcag
     3001 gacttgctgg acatcatcaa ccagtttggc ggccagatga agcagaccta cggggtgccg
     3061 gtgaaggaga ttcaggaagc catcaagcac ggcgtgcgca agatcaacat cgacaccgac
     3121 atccgcatgg ccatgaccgg cgcggtgcgc aaattccagg ccgagaaccc cgacaagttc
     3181 gacatgcgcg aatggatgaa gcccgcgcgc gaagccgcca aggccatctg caagcagcgc
     3241 tacatcgaat tcggctgcga aggccagggc gccaagatca agggcgtgcc gctgtcggtg
     3301 atggccgag
//
LOCUS       DAOMVO010000278         3290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8520_length_3290_cov_10.727975, whole genome shotgun sequence.
ACCESSION   DAOMVO010000278 DAOMVO010000000
VERSION     DAOMVO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8520_length_3290_cov_10.727975"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKV30_11390"
     CDS             complement(<1..231)
                     /locus_tag="PKV30_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL30847.1"
                     /translation="MSDQWKFETQSVHAGYTPDPTTKAVAVPIYQTVAYAFDSAQHGA
                     DLFDLKVAGNIYSRIMNPTCDVLEKRVAALEGG"
     gene            complement(327..1427)
                     /locus_tag="PKV30_11395"
     CDS             complement(327..1427)
                     /locus_tag="PKV30_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL30848.1"
                     /translation="MTNLPPYPLDATLFARAQALLDEEWLAKDADLAPVLPTVLSRGV
                     GQDWHKAGTFRHHLVGVARSLAMWQQPRDVRLLGLLHSVYGNAFVDLVKFDASTERAG
                     LQALIGDEAEELVYAFCTSSRREFIEKALAGQIEADGSVPLSRNDGGRATLPARTVAA
                     FLIVSMADTIEQWSSWQDDVYSRFPTPNDQRPQAVHWAASLWPGPMRPSGRMLHQINR
                     LGLALQHPALKNLLPMPPVFDHCTQALSTADDAAGAALYWSVVQQDVPLVDLDVATGV
                     LEQAVRHNPWVGEPRMVLAQLYLSAGRAEDAAQAAESALAAFCAWGNSWDKRVAWEAW
                     VAWTRILRQSAQTGTWPARLDKLNNLALRDGG"
     gene            complement(1612..2034)
                     /locus_tag="PKV30_11400"
     CDS             complement(1612..2034)
                     /locus_tag="PKV30_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRL30849.1"
                     /translation="MATNPESILWPMLALMLWTLCVLLLLPLARYRAIRAGAVDWDDF
                     APGESARVPEPARRVNRALVNLLEVPVIFYALVLACLMTARVDATALALAWAYVALRM
                     AHTIVHLSYNRVTHRFIVFAASNGVLVAMLLRLAWRLK"
     gene            2137..2409
                     /locus_tag="PKV30_11405"
     CDS             2137..2409
                     /locus_tag="PKV30_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HRL30850.1"
                     /translation="MARMVNCIKLGTEAEGLDFPPYPGELGKRIWESVSKQAWQDWLK
                     HQTMLVNENRLNLADQRAREYLKRQMENHFFGAGADAAAGYVPPSA"
     gene            2498..>3290
                     /gene="mnmD"
                     /locus_tag="PKV30_11410"
     CDS             2498..>3290
                     /gene="mnmD"
                     /locus_tag="PKV30_11410"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRL30851.1"
                     /translation="MADPVVRWRDDGTPFSPRFGDVYRSAGPAGALAQARQVFLHGCG
                     LLPTPGQEAIWRGAPRWQVLEAGFGLGLNFLTTWHAWRQAAERPRQLFYTAVEAFPPE
                     PDDLRRSAAPFPELAPLAESLIAPWHGLLPGVHRLGLDGGQVQLTLAIGDVQPMLAEL
                     TGEYDSVFLDGFDPACNPEMWRLHTLRAIARLARRGARLATRCVADEVRERLVACGFE
                     VEGTTAAGSPPALRARYAPRWTPRPRRVGDEAPMVAQPARCAVVGA"
BASE COUNT          521 a         1105 c         1161 g          503 t
ORIGIN      
        1 gccgccttcc agcgccgcca cgcgcttttc gagcacgtcg caggtcgggt tcatgatgcg
       61 gctgtagatg ttgccggcca ccttcaaatc gaacagatcg gcgccgtgct gcgcgctgtc
      121 gaaagcgtag gccaccgttt ggtagatggg cacggcgacg gccttggtgg tcgggtcggg
      181 cgtgtagccg gcgtgcacgg attgggtttc aaacttccat tggtcggaca tggggtgctc
      241 cttcaagggt gcaaataact attaaaaccg tagctgctct cgcttgtcag aaaagcgctg
      301 gcggccgatt tgacttgtat ttttcgtcaa ccgccgtccc gcagcgccag gttgttcagc
      361 ttgtccagcc gcgcgggcca ggtgcccgtc tgcgcgctct ggcgcaggat gcgcgtccag
      421 gcgacccagg cttcccaggc cacgcgcttg tcccacgaat tgccccaggc gcagaaggcg
      481 gccagcgcgc tttcggcggc ctgcgccgca tcttcagcac ggccggcgga caggtacagc
      541 tgcgccagca ccatgcgcgg ctcgccgacc caggggttgt ggcgcacggc ctgctccagc
      601 acgccggtgg ccacgtccag atcgaccagc ggcacatcct gctgcaccac cgaccagtac
      661 agcgcggcgc ccgcggcgtc gtcggcggtg ctcagggctt gagtgcagtg gtcgaacacc
      721 ggcggcatgg gcagcaggtt tttgagcgcc gggtgctgca gcgccagacc cagccggttg
      781 atctggtgca gcatgcggcc cgaggggcgc atcggccccg gccacagcga cgcggcccag
      841 tgcacggctt gcgggcgctg gtcgttgggc gtgggaaagc gcgaatacac gtcgtcttgc
      901 cagctcgacc actgctcgat ggtgtcggcc atgctgacga tgagaaacgc cgccacggtg
      961 cgcgccggca gcgtcgcgcg gccaccgtcg ttgcgcgaca ggggcacgct gccgtcggcc
     1021 tcgatctggc cggccagcgc tttttcgatg aattcgcggc gcgatgaggt gcaaaaggcg
     1081 tagaccagtt cttcagcctc gtcgccaatc agcgcctgca ggccggcgcg ctcggtggac
     1141 gcatcgaact tcaccagatc gacaaacgcg ttgccgtaca cgctgtgcag caagcccagc
     1201 aggcgcacgt cgcgcggctg ctgccacatc gccaacgagc gcgccacgcc gacgaggtgg
     1261 tggcggaagg tgccggcctt gtgccagtcc tgccccacgc cgcgcgagag cacggtgggc
     1321 agcacgggcg ccaggtcggc gtccttggcc agccattctt cgtccagcag cgcttgggcg
     1381 cgggcgaaca gggtggcgtc gagtgggtat ggcggcaggt tggtcatatc tcaggtctgt
     1441 catgttatcc acggcactgg cgcatcgccc tcgggcgaca aacccaaaac agccagaagg
     1501 ccgcggagca ggccatgcgc gggcgccgtg gccgggcttg caccggccac tggcgacgtc
     1561 cccctcccga agagagaggg ggaagacgcc gaaggcggct cagggggtgt ttcatttgag
     1621 gcgccacgcc aggcgtaaca gcatagccac cagcacccca ttgctggcgg caaacacgat
     1681 gaagcggtgc gtgacgcggt tgtagctcag gtggacgatg gtatgcgcca tgcgcagcgc
     1741 cacgtaggcc caggccagcg ccagcgccgt ggcgtcgacg cgcgcggtca tcaggcaggc
     1801 cagcaccagc gcgtagaaga taaccggcac ctccagcagg ttgaccagcg cgcggttgac
     1861 gcggcgcgcc ggctcgggca cgcgggccga ttcgccgggc gcaaagtcgt cccagtccac
     1921 cgcgccggcc cgaatcgccc gataacgtgc cagcggcaac agcagcagca cgcacagcgt
     1981 ccacagcatc agcgccagca tgggccacag gatggattcg gggttggtcg ccacgggcgg
     2041 gagtgtatcg accgtgcggg cgctcggggc acatgaagct tgctacagtg cggcatggcg
     2101 cccgcgcggc gcggtcagca tctgaaggac aaacgcatgg cacgcatggt caactgcatc
     2161 aagctcggca ccgaggcgga ggggctggac tttccgccct acccgggcga actgggcaag
     2221 cgcatctggg aaagcgtcag caagcaggcg tggcaagact ggctcaagca ccagaccatg
     2281 ctggtgaacg aaaaccgcct caacctggcc gaccagcggg cgcgcgagta cctcaagcgg
     2341 cagatggaga atcatttctt cggcgccggt gccgacgcgg ctgcggggta cgtgccgccg
     2401 agcgcctgat tcgctcgggc cgggcgcccg attttcaatt caaatgggcc gctggcgctt
     2461 gacagacaag cgcgagcagc tatcaattca atagttcatg gccgacccgg tggtgcgctg
     2521 gcgcgacgac ggcacgccgt tcagcccgcg ctttggcgac gtctaccgca gcgccgggcc
     2581 cgccggcgcg ctggcgcagg cgcggcaggt ttttctgcac ggctgcgggc tgctgccgac
     2641 gcccgggcaa gaggccatct ggcgcggcgc gccacgctgg caggtgctgg aggccggctt
     2701 tggcctcggc ctcaactttc tcaccacctg gcacgcgtgg cggcaggcgg ccgagcggcc
     2761 gcgccagttg ttttacacgg cggtcgaagc ctttccgccg gagcccgatg acctgcggcg
     2821 cagcgccgcg cccttccccg aactggcgcc gctggccgaa tcgctgatcg ccccatggca
     2881 cggcctgctg cccggcgtgc accggctggg gctggacggc gggcaggtgc agctgacgct
     2941 cgccatcggc gacgtgcagc ccatgctggc cgagctgacg ggcgagtacg acagcgtttt
     3001 tctcgacggc ttcgatcccg cgtgcaaccc cgagatgtgg cgcctgcaca cgctgcgcgc
     3061 catcgcacgg ctggcgcgcc gtggtgcacg cttggcgacg cggtgcgtgg cggacgaggt
     3121 gcgcgagcgg ctcgtcgcct gcggttttga ggtcgaaggc accaccgcag cggggtcgcc
     3181 ccccgcgctg cgcgcgcgtt acgccccgcg ctggacgccg cgcccgcgcc gcgtgggcga
     3241 tgaggcgccc atggtggcgc agccggcgcg ctgtgccgtc gtcggcgccg
//
LOCUS       DAOMVO010000279         3284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8543_length_3284_cov_14.348405, whole genome shotgun sequence.
ACCESSION   DAOMVO010000279 DAOMVO010000000
VERSION     DAOMVO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_3284_cov_14.348405"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="PKV30_11415"
     CDS             <1..97
                     /locus_tag="PKV30_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRL30852.1"
                     /translation="WEDFILRNRRILADQMEFLMAHLPKTSRLHG"
     gene            complement(123..902)
                     /locus_tag="PKV30_11420"
     CDS             complement(123..902)
                     /locus_tag="PKV30_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30853.1"
                     /translation="MDEIETLGKMNSTQRWHRVSALGLCLGALAAPALAQTPPNLLTN
                     AGFEDNPLPALVCGNNYPKPITPWVMLSGSQTNVIAVDGGANCNYGNSGPALDAQNTP
                     QGTWQHYLDLLATGTVYQTFTVPSCPGGGAAPRTASFSGYFSSRDGSSSGSGRIAIVS
                     GAGAGGAELAAASTTPGGLSYQSWTPVSGDVPLTPGSTFSYVVSMTNPTNFDHASVTL
                     SACPVVSTPTAVPANDPWALLLAAAGVGWLARRRLARQQRG"
     gene            1204..1782
                     /locus_tag="PKV30_11425"
     CDS             1204..1782
                     /locus_tag="PKV30_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL30854.1"
                     /translation="MPRLIFFCGHAGTGKTTLAKRALPALHRATGESFCLLDKDTLYG
                     DYSSAVMGVLTGDPNDRDSPPYLQHLREPEYAGLLKTARENLALGVNCIVVGPLSREV
                     RAHKLTDRAWLGVGDEVQVRIVWVHLREDEARQRIAARGNPNDAWKLAHWDDYRTRLF
                     MPSAQDHPELLLLDNSEPAERVFETLMTALGG"
     gene            complement(1876..3261)
                     /gene="mdtD"
                     /locus_tag="PKV30_11430"
     CDS             complement(1876..3261)
                     /gene="mdtD"
                     /locus_tag="PKV30_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HRL30855.1"
                     /translation="MLWLVAIGFFMQTLDSTIVNTALPAMARSLGESPLRMQMVVVAY
                     ALTMAVIIPASGWLADRFGTQRIFLLAIVLFTLGSAACAASGSLNQLIAARVLQGTGG
                     AMLLPVGRLTVLRNFPNEQFLAAMSFVAIPGLIGPLIGPTLGGWLVEVASWHWIFLIN
                     LPVGLIGAIATLRLMPNQRTPDIGRFDLGGYLLLTVAMVAISLSLDGMGALSWARTLV
                     VLLLVIGLAALAAYWLHALKSAAPLFSPALFAIASYRVGVLGNLFARIGSGAMPFLLP
                     LLMQLVMGFSPAQAGMLMLPVALASMATKRLVTRIITRWGYRRVLVTNTLLLGVMIAG
                     FALMTPEQPLLLRIVQLALFGAVNSMQFTAMNAVTLKDLAPARAASGNSLFSMVQMLG
                     MSLGVTCAAALLHAFTAWLQTGAGADALPAFRAALALIGVMTMASAAIFWQLDRGTPV
                     RSSAAAAVDET"
BASE COUNT          599 a         1091 c         1114 g          480 t
ORIGIN      
        1 gtgggaagac ttcatcctgc gcaaccgccg catcctggcg gaccagatgg agttcttgat
       61 ggcgcatctg cccaagacct cgcgcctgca cggctgaggt gcggcgagga actgggcaaa
      121 ccctagcccc gttgctggcg agccagcctg cgccgggcca gccagccgac ccccgcggcg
      181 gccagcaaca aggcccacgg atcatttgcc ggcacagcgg tgggcgtcga taccaccgga
      241 caggcgctga gcgtgaccga ggcgtggtcg aagttggtgg gattggtcat ggacacgacg
      301 taagagaacg tgctcccagg cgtcaggggc acgtcgccgc tcactggtgt ccacgactgg
      361 tagctgagac cgccaggcgt tgtgctggcc gcagccaact ccgcgccgcc tgcgcctgca
      421 ccggacacga tggcgatgcg ccctgaacca gagctgctgc catcgcggct cgagaagtag
      481 cccgagaaac tggcggtgcg tggcgccgcg ccgccgccag ggcaagaggg cacggtgaag
      541 gtttggtaga ccgtgccggt cgccagcaga tcaaggtaat gctgccaggt gccttgcggc
      601 gtgttctggg cgtcaagcgc ggggccgctg ttgccgtagt tgcagttggc gccaccgtcg
      661 accgcgatga cgttggtctg gctgccgctg agcatgaccc acggtgtgat gggcttgggg
      721 tagttgttgc cgcaaacgag agcggggagc gggttgtctt cgaagccggc attggtcagc
      781 aggttcggtg gcgtctgcgc cagcgccggc gcggcgaggg cgcccaagca cagcccgagc
      841 gccgatacac gatgccagcg ctgagtcgaa ttcattttgc ccaaggtttc aatttcgtcc
      901 atcttgtctc ccgtggtccc tttattgttt ccgccgatgc accttggcgc gcctcggccc
      961 ctgatcggcg cagcgtaacc gccaccgacc acaatccgca ccatgttgca gagcgttacc
     1021 tgctgatggg atcacgatcc gggcagtcgc tgccggcgcc tgtgcgggtc acttcgatca
     1081 tagatgctac cgggggtttg gtcatcggcc ttttcttgcc caaccagtgc agcttggcgg
     1141 cccgccgaga acaccgcatc cagaagaaga accagccggg cggcgccggg catactgcgc
     1201 gccatgcccc gcctcatctt cttctgcggc cacgccggca ccggcaagac cacgctggcc
     1261 aagcgcgcgt tgccagcgct gcaccgcgcc accggcgaat cgttttgcct gctcgacaag
     1321 gacacgctct atggcgacta cagctcggcc gtgatgggcg tgctgacggg cgacccgaac
     1381 gaccgcgaca gcccgccgta cctgcagcac ctgcgcgagc cggaatacgc ggggctgctg
     1441 aaaacggcgc gcgaaaacct ggcgctgggc gtcaactgca tcgtcgtcgg cccgctgtcg
     1501 cgcgaggtgc gggcgcacaa gctgaccgac cgtgcgtggc tgggcgtcgg cgatgaagtg
     1561 caggtgcgca tcgtctgggt gcatctgcgc gaagacgagg cccggcagcg catcgccgcg
     1621 cgcggcaacc ccaacgatgc gtggaagctg gcgcactggg acgactaccg cacgcggctg
     1681 ttcatgccca gcgcgcaaga ccatcccgag ctgctgctgc ttgacaacag cgagccggcc
     1741 gagcgggtct tcgagacctt gatgacggcg ctgggtggtt gagttttttg aggattaaaa
     1801 aggccgccag cgcttgccca tcaagcgcga acagctacta aaaagatagt atgacgacgt
     1861 gcgtggtcgc acgcatcaag tttcatccac cgcggctgcg gccgaagatc gcaccggcgt
     1921 gccgcgatcc agctgccaga agatcgccgc cgacgccatg gtcatgacgc cgatcagcgc
     1981 cagcgcggcg cgaaaggcgg gcagggcgtc ggcccccgcg cccgtctgca gccaggccgt
     2041 gaaggcgtgc agcagcgcgg ccgcgcacgt cacgcccagg ctcatgccca gcatctgcac
     2101 catcgagaac aggctgttgc cgctggccgc gcgcgcgggg gccaggtctt tcagcgtgac
     2161 cgcgttcatg gcggtgaact gcatcgagtt gacggcgcca aacagcgcca gttgcacgat
     2221 gcgcagcagc agcggctgct cgggcgtcat cagagcgaag ccggcgatca tcacgcccag
     2281 cagcagcgtg ttggtgacca gcacgcgccg gtagccccag cgcgtgatga tgcgcgtgac
     2341 caggcgcttg gtggccatgc tggccagcgc caccggcagc atcagcatgc cggcctgcgc
     2401 gggcgaaaaa cccatcacca gctgcatcag cagcggcagc aggaaaggca tggcgcccga
     2461 gccgatgcgc gcaaacaggt tgcccagcac gcccacgcgg tagctggcaa tggcgaacag
     2521 cgccggcgaa aacagcggcg ccgcgctttt gagcgcgtgc agccagtagg ccgccagcgc
     2581 ggccagcccg atcaccagca acagcacgac gagcgtgcgc gcccagctca aggcccccat
     2641 gccgtcgagc gagagcgaga tcgccaccat ggccaccgtc agcagcagat aaccgccgag
     2701 gtcgaagcgg ccgatgtcgg gcgtgcgctg gttgggcatc aggcgcagcg tggcgatcgc
     2761 gccgatcagc ccgacgggca ggttgatcag aaagatccag tgccacgaag ccacttccac
     2821 cagccagccg cccagcgtcg gcccgatcag cgggccgatc agaccgggga tggcgacaaa
     2881 gctcatcgcc gccaggaact gctcgtttgg aaagttgcgc agcaccgtta gccgccccac
     2941 cggcagcagc atggcgccgc ccgtgccctg cagcacgcgc gcggcgatca gctgattgag
     3001 gctgcccgac gccgcgcacg cggctgagcc gagcgtgaac agtacgatcg ccagcagaaa
     3061 gatgcgctgc gtgccaaagc ggtcggccag ccagccgctg gccgggatga tcaccgccat
     3121 ggtcagcgca taagccacca ccaccatctg catgcgcagc gggctctcgc ccaggctgcg
     3181 cgccatggcg ggcagggcgg tgttgacgat ggtcgaatcc agcgtctgca tgaaaaagcc
     3241 gatggcgacc agccagagca gggcgcgggg cgacaggggg cggt
//
LOCUS       DAOMVO010000280         3264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8624_length_3264_cov_14.255531, whole genome shotgun sequence.
ACCESSION   DAOMVO010000280 DAOMVO010000000
VERSION     DAOMVO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8624_length_3264_cov_14.255531"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKV30_11435"
     CDS             complement(<1..665)
                     /locus_tag="PKV30_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL30856.1"
                     /translation="MNFSAWSIRNPVPTLMLFLLLTLGGLFSFHQMKVQNFPDIDMPM
                     VSVVAALPGATPGQLENEVARKIENTLATVQGVKHISTTIVDGSVSIMVEFRLEKDLQ
                     EALDDVRSAVSRVRGDLPSDLREPIVAKLDLATQPMLAYALSSKRMGDADLSWFVDND
                     VSKRLLAIPGVGAVNRVGGADRQVQVALDPARLQGLGITAAEVSRQLRQVQMESAGGR
                     VDLG"
     gene            complement(692..1849)
                     /locus_tag="PKV30_11440"
     CDS             complement(692..1849)
                     /locus_tag="PKV30_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL30857.1"
                     /translation="MKPSSFLLLLAAALTLAACGKKEGAANGPQAAASAPRPALTVTV
                     ARPERQNLTREITANGNIAAWQEASVGADVSGLRLADVRVNVGDVVKKGQVLANFDAA
                     PVQQDQAQARASLAEAEAAYADARGNAERARAVQSSGALSQQQINQYVTAEKTARARV
                     EAARAVVGSQAIRVRNTQVLAPDAGVISARAATVGQVVAPGTELFRMVRQGRLEWRGE
                     VMAEDLPAIHPGQSVRIDLPSAPDADGPVVTGTVRQLAPTVDPKTRFAIVYVDLPRGG
                     AARAGMFARGRIAIGQSDALTVPQDAIVMRDGFAHVLKLGEGDRVQLLRVQTGRLADG
                     RVEITQGLDANARVVVRGGAFLNDGDLVQVVADSVPKVPSTPASPAQAASK"
     gene            complement(1927..3264)
                     /locus_tag="PKV30_11445"
     CDS             complement(1927..3264)
                     /locus_tag="PKV30_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRL30858.1"
                     /translation="MAPPAWIAPLPHAGSAVALADWWRAAGDATLTELIDAALQDNPS
                     LAAAEARVAQARAQVGSQRAGLLPQVGAAASASRGNAGGVGSTPPGVDPPVATTLQAG
                     VQASWEIDLFGRQRALVHAASARAEGAEALAHLARVALVGDVASTYHGLRLCQALLDT
                     ARQDLASRFETARLALISRDAGFTAPATAALADASAADGRSRTTQQQAACDVQRKALV
                     ALTGLPEPDIEQKMAVAQVQQAPAALFSIASLPADTLRQRPDVWAAEREVLAARAEIV
                     SADAARWPGLSLGGNIGALQLRAGGATRNFSTWQFGPLQLSLPIFDGGRIAAGQAAAR
                     ARYDEAVANYQGQVRQAVQEVETALTQGAAARAREADARAAVAGYRQQFAATEALYRQ
                     GMANLPQLEEARRNLLVSEITLAQLLNDQQAAGVALYRAAGGGWQAPAGGAAP"
BASE COUNT          432 a         1165 c         1178 g          489 t
ORIGIN      
        1 cccagatcga cgcggccgcc ggcgctttcc atctgcactt ggcgcaactg gcgcgacacc
       61 tcggccgcgg tgatgcccag accttgcagg cgcgccggat cgagagcgac ctgcacctgc
      121 cgatccgccc cgcccacgcg gttgaccgcg cccacgccgg ggatggccag cagccgcttg
      181 gacacgtcgt tatcgacaaa ccacgacagg tcggcatcgc ccatgcgctt ggacgacagc
      241 gcataggcca gcatcggctg cgtcgccagg tcgagcttgg cgacgatggg ctcgcgcagg
      301 tcgctcggca aatcgccccg cacgcgcgag acggccgagc gcacgtcgtc cagcgcttcc
      361 tgcaaatctt tttcgagccg gaactcgacc atgatcgaca ccgagccatc gacgatggtg
      421 gtgctgatgt gcttgacgcc ctgcaccgtg gccagggtgt tctcgatctt gcgcgcgacc
      481 tcgttctcca gctgcccggg cgtggcgccc ggcagcgcgg ccaccacgct gaccatcggc
      541 atgtcgatgt cgggaaagtt ctgcaccttc atctggtgaa agctgaacaa gccccccagc
      601 gtgaggagca ggaacagcat cagcgtcggc accgggttgc ggatcgacca ggcggagaag
      661 ttcatggggc ggcgccttgt ggattcgcta cttatttgct agctgcttgc gccggactgg
      721 cgggcgtcga cggcactttt ggcactgaat cggccaccac ctgcaccaga tcgccgtcgt
      781 tcaggaaggc gccgccgcgc accaccacgc gggcgttggc gtccagcccc tgcgtgattt
      841 ccacgcggcc atcggccagc cggccggtct gcacgcgcag cagttgcacg cggtcgccct
      901 cgcccagctt cagcacgtgg gcaaaaccgt cgcgcatgac gatggcgtct tgcggcaccg
      961 tgagcgcatc gctctggccg atggcgatgc gcccgcgcgc aaacatgccg gcgcgcgccg
     1021 cgccgccgcg cggcaaatcg acgtagacga tggcaaagcg cgtcttgggg tcgacggtgg
     1081 gcgccagttg ccgcaccgtg ccggtgacga cggggccatc ggcatcgggc gcgctgggca
     1141 ggtcgatgcg caccgactgg ccggggtgga tggcgggcag gtcttctgcc atcacctcac
     1201 cgcgccattc gagccggccc tggcgcacca tgcgaaacag ctcggtaccg ggcgccacca
     1261 cctggccgac ggtggcggcg cgcgccgaga tgacgccggc atcgggcgcc agcacctgcg
     1321 tgttgcgcac gcgaatcgcc tggctgccga ccacggcgcg cgccgcctcg acgcgggcgc
     1381 gggcggtttt ctcggccgtc acgtactggt tgatctgttg ctggctcagg gcgccgctcg
     1441 actgcacggc gcgtgcgcgt tcggcgttgc cgcgcgcgtc ggcgtaggcg gcttcggcct
     1501 cggccaggct ggcgcgggcc tgcgcctggt cttgctggac cggcgcggcg tcaaaattgg
     1561 ccagcacctg gcccttcttg accacgtcgc ccacgttcac gcgcacgtcg gccagccgca
     1621 ggccagacac gtcggcaccc acgctggctt cttgccaggc ggcgatgttg ccgttggcgg
     1681 tgatctcgcg cgtgaggttc tggcgctcgg ggcgagccac ggtcacggtg agcgcggggc
     1741 gcggggcgct ggcggcggct tgggggccgt tggccgcgcc ttccttcttg ccgcaggcgg
     1801 cgagcgtcag ggcggcggcg agcaagagca gaaacgaact gggcttcatg aatggtttcc
     1861 ggcgccaggc atcgctggcg cattgatcaa tgggcggcca acgccgatct ggcgcggggt
     1921 gcccgctcat ggcgccgcgc ccccggccgg tgcctgccag ccgccacccg ccgcgcggta
     1981 cagcgctacg ccggcggcct gctggtcgtt aagcaactgc gccagcgtga tttcagacac
     2041 cagcaggtta cggcgcgctt cttcaagctg cggcaggttg gccataccct ggcggtacag
     2101 cgcctcggtg gcggcaaact gctggcggta gccggccacg gcggcgcggg cgtcggcctc
     2161 gcgcgcgcgg gcggcggcgc cttgcgtgag ggccgtctcc acctcttgca cggcctggcg
     2221 cacctggccc tggtagttgg cgaccgcctc gtcgtagcgc gcccgcgcgg cggcctggcc
     2281 ggctgcaatg cggccgccgt cgaagatcgg caggctgagc tgcagcggcc cgaattgcca
     2341 ggtcgaaaag ttgcgcgtgg cgccgccagc gcgcaactgc agcgcgccga tgttgccgcc
     2401 caggctcaag cccggccagc gcgcggcgtc ggcggagacg atctcggccc gtgcggccag
     2461 cacctcgcgc tcggcggccc acacgtcggg gcgctggcgc agggtgtcgg cgggcagtga
     2521 cgctattgaa aaaagagctg ctggcgcttg ctgcacctga gccacggcca ttttttgttc
     2581 aatgtccggc tcgggcaggc cggtcagcgc gaccagcgcc ttgcgctgca cgtcgcacgc
     2641 cgcctgctgc tgcgtggtgc gcgagcggcc atcggcggcg ctggcgtcgg cgagagccgc
     2701 ggtggctggc gcggtgaagc cggcgtcgcg gctgatgagc gccagccgtg cggtttcgaa
     2761 gcgcgaggca aggtcctgcc gggcagtgtc gagcagcgcc tggcacaggc gcaggccgtg
     2821 gtacgtgctg gccacgtcgc ccaccagcgc cacgcgcgcc aggtgcgcca gcgcctcggc
     2881 gccctcggca cgcgcgctgg ctgcgtggac gagcgcgcgt tggcggccga acagatcgat
     2941 ctcccaactc gcctgcacgc cggcttgcag cgtggtggcc accggcggat cgacgcccgg
     3001 cggcgtgctg cccacgccgc ccgcgttgcc gcgactggcg ctggcggcgg cgcccacctg
     3061 cggcagcagc cccgcgcgct ggctgcccac ctgcgcgcgc gcctgcgcca cgcgcgcctc
     3121 ggcggcggcg aggctcgggt tgtcttgcag cgcggcgtcg atcaattcgg tcagcgtggc
     3181 atcgcccgcc gcacgccacc agtcggccaa cgccaccgcg ctgccggcgt gcggcagcgg
     3241 cgcgatccag gctggcggcg cgat
//
LOCUS       DAOMVO010000281         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8794_length_3217_cov_14.621126, whole genome shotgun sequence.
ACCESSION   DAOMVO010000281 DAOMVO010000000
VERSION     DAOMVO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8794_length_3217_cov_14.621126"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1194)
                     /locus_tag="PKV30_11450"
     CDS             complement(<1..>1194)
                     /locus_tag="PKV30_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL30859.1"
                     /translation="DLLAAHVQAQLTAEQLAEERMHERLRARLLNQVQYAVQPAQVLM
                     AEAAEIARVFDADAVLATNHAGLHCHGDVPVALRAQLLRWLMAQELAGEPVLVTSSLS
                     ELAPELREHLDGWAGLLAISYQPRAQAALVLLRCEQTETIDWGHDPAKATVVGPLGPR
                     LTPPGSFQLWRETVQGRAEPWTATQVAAMGQLRADLSRIVVTRAAELERQRAAVASVL
                     DSHGRDGRMPEDAGRIERLLRQGRMALAREPLDVAALVAERLEAAQRRHGGIAAFLER
                     PGKAPQFTAVPVQGDPARLAQLLDNLLDNAAQHGAVGEALVIRVETQDAFAVIEVSNI
                     SPPIDPQLVARLFNPLVSAAAGDASAGLGYGLYVCQAIAVAHGGDLAYTYEDPFVTMT
                     ARLPLV"
     assembly_gap    1195..1294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1295..1753)
                     /locus_tag="PKV30_11455"
     CDS             complement(<1295..1753)
                     /locus_tag="PKV30_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRL30860.1"
                     /translation="MFLGLLQDAVHMVRELTGFDRVMAYRFRADDSGDIVAEERDAAL
                     DPYLGWRFPASDIPAQARRLYIENTMRLIADVGSTPVAVRAAPGVDQPLDMSHGVLRS
                     VSPIHIEYLQNIGVGASMSLSIVLEGRLWGMLACHHRTPRQVPYVTRAACD"
     gene            1806..2264
                     /locus_tag="PKV30_11460"
     CDS             1806..2264
                     /locus_tag="PKV30_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30861.1"
                     /translation="MRAIRRWAAFELGHQPALHGVQQQIEPAPANLDGGVARLRRVGG
                     RGRPGRLREQRVHGRVFKVRRRGDFAQAGRTGQQGGQVVAGVGHAQIGIQRHQGAVRL
                     DETGNVDRLAVAIAQIHRRRRSGGGRAQRRGHARLSWAAGAAASARRCNS"
     gene            complement(2204..2839)
                     /locus_tag="PKV30_11465"
     CDS             complement(2204..2839)
                     /locus_tag="PKV30_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HRL30862.1"
                     /translation="MSPTPLAAEPTEQLGDRVNLRAVLRAATADLHAHIDRAMPLARS
                     APTLADYLTHLLLLRDWLGALGQSMSGLNGLADEQVAVLADAQQAAALIGQTSPVPRD
                     AAGPEQAALRALDNGAHADAARLGVRYVIEGSHLGGKVLRRRWGERLAPHPLGYLGGG
                     APDRWPAFLNELAAARSPDERAAACAGAVLAFQLLQRLADAAAPAAHESRA"
     gene            complement(2904..>3217)
                     /locus_tag="PKV30_11470"
     CDS             complement(2904..>3217)
                     /locus_tag="PKV30_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRL30863.1"
                     /translation="GVHESTISRVTTAKYMATPRGTFELKYFFGSALGTEAGGNASST
                     AVRALIKQLIAAEDPAKPLSDSQLADMLKEQGIDCARRTVAKYREALRIPVATLRKAV
                     A"
BASE COUNT          438 a         1096 c         1132 g          451 t
ORIGIN      
        1 accagcggca gccgcgccgt catggtcacg aacgggtctt cgtaggtgta ggccagatcg
       61 cccccatgcg ccacggcaat ggcctggcac acgtacagcc catagcccag ccccgcgctg
      121 gcatcgccgg cggcagccga caccagcggg ttgaacagcc tcgccaccag ttgcggatcg
      181 atgggcgggc tgatgttgct gacctcaatg acggcaaacg cgtcctgtgt ttcaacgcgg
      241 atcaccagcg cctcgcccac ggcgccgtgc tgcgcggcgt tgtcgagcag gttgtcgagc
      301 aactgcgcca gccgcgccgg gtcgccctgc accggcaccg cggtgaattg cggcgccttg
      361 cccggccgct ccagaaacgc ggcaataccg ccgtggcggc gctgcgccgc ctccagccgc
      421 tcggcgacca gggccgccac gtcgagcggc tcgcgggcca gcgccatgcg gccctggcgc
      481 agcagccgct cgatgcggcc ggcgtcctcc ggcatgcggc catcgcggcc atggctgtcc
      541 agcaccgagg ccacggcggc gcgctggcgc tccagctcgg ccgcgcgcgt gaccacgatg
      601 cggctcaggt cggcgcgcaa ctggcccatg gcggcgacct gcgtggcggt ccagggctcg
      661 gcgcggccct gcacggtttc gcgccacagc tggaacgagc cgggcggcgt caaccggggc
      721 cccagcgggc cgaccaccgt ggccttggcc gggtcgtggc cccagtcgat ggtttcggtc
      781 tgttcgcagc gcagcagcac caatgccgcc tgcgcgcgcg gctggtagct gatggccagc
      841 aagccggccc agccgtccag gtgttcgcgc aattccggcg ccagctcgga cagcgaggag
      901 gtcaccagca cgggctcgcc ggccagctct tgcgccatca gccagcgcag cagttgcgcg
      961 cgcagcgcca ccggcacatc gccgtggcag tgcaggccgg cgtggttggt ggccagcact
     1021 gcgtcggcgt cgaacacgcg ggcgatctcg gccgcttcgg ccatcagcac ctgagcgggt
     1081 tgcacggcgt attgcacctg gttgagcagg cgcgcgcgca gccgctcgtg catgcgctct
     1141 tcggccaact gctcggccgt cagttgggcc tgcacatgcg cggccagcaa gtcgnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcgca cgcggcccgc gtgacgtaag
     1321 gcacctggcg cggcgtgcgg tggtggcagg ccagcatgcc ccacaggcgc ccttccagca
     1381 cgatggacag gctcatcgac gcaccgacgc cgatgttctg caaatattcg atgtggatcg
     1441 gcgacacgct gcgcagcacg ccgtggctca tgtccagcgg ctgatcgacg cccggcgcgg
     1501 cgcgcaccgc caccggcgtg ctgcccacgt cggcgatcag gcgcatcgtg ttttcgatgt
     1561 acaggcggcg cgcctgcgcc ggaatgtcgc tggccggaaa gcgccagccc aggtaggggt
     1621 cgagcgccgc atcgcgctcc tcggccacga tgtcgccgct gtcgtcggcg cgaaagcggt
     1681 aggccatcac gcggtcgaag ccggtcagct cgcgcaccat gtgcacggcg tcttgcagca
     1741 ggcccaaaaa catccgtctg ttgccgaaag cgcttgaccg cggcgtgcag gcccggcccg
     1801 tagtcgtgcg cgccatccgc cggtgggcgg ctttcgaact cggccatcag ccggccttgc
     1861 acggcgtgca acagcagatc gaaccggcgc ccgccaacct ggatggcggt gtggcgcgac
     1921 tgcggcgcgt cggcgggcgc ggtcgaccgg gccgcctgcg tgagcagcgc gtgcacggcc
     1981 gggtcttcaa agtgcgccgg cgtggcgact tcgcccaggc cgggcgcacc gggcagcagg
     2041 gtggccaggt tgtcgctggc gtaggtcacg cgcagatcgg catccagcgc catcagggcg
     2101 ccgtgcggct ggatgagacc gggaatgtgg atcggctcgc ggtcgcaatt gctcaaatcc
     2161 accgccggcg ccgcagcggt ggcgggcgcg cccagcggcg cggtcatgcg cgactttcgt
     2221 gggcggccgg cgcggcggcg tccgccaggc gctgcaacag ctgaaacgcc agcacggcgc
     2281 ccgcgcaggc ggcggcacgc tcgtcaggcg accgtgccgc cgccagttca ttcaggaagg
     2341 ccggccatcg atccggcgcc ccaccgccca gatagcccag cgggtgcggc gccaaccgct
     2401 cgccccagcg gcggcgcagg accttgccgc ccaggtgtga gccttcaatg acgtaacgca
     2461 cgcccagtcg cgccgcgtcg gcgtgtgcgc cgttgtccag cgcgcgcagc gccgcctgct
     2521 cggggccagc cgcgtcgcgc ggcaccggcg aggtttggcc gatcagcgcg gcggcttgtt
     2581 gggcatcggc caggacggcg acctgctcgt cggccaggcc attgagcccg ctcatggatt
     2641 gccccagcgc gccaagccag tcgcgcagca gcagcaggtg tgtcaggtaa tcggccaggg
     2701 tcggcgcgga ccgtgccagc ggcatggcgc ggtcgatgtg ggcgtgcaga tcggcggtgg
     2761 cagcgcgcag cacggcgcgc agattgaccc tgtcgccgag ttgttccgta ggctcagcag
     2821 cgaggggggt tggggacatg agagggtcag tgtaatccgc agcctgccgt ggcgttgtgt
     2881 gaaatcacat gcctgcgccg gaatcaggcc accgccttgc gcagcgtcgc caccggaatg
     2941 cgcagggctt cgcggtactt ggcgacggtg cggcgcgcgc agtcgatgcc ctgctctttc
     3001 agcatgtcgg ccagctggct gtcggacagc ggcttggccg ggtcttcggc ggcgatgagc
     3061 tgcttgatga gcgcgcgcac cgccgtgctg ctggcgttgc cgccggcttc ggtgccgagc
     3121 gccgagccga agaagtactt cagctcgaag gtgccgcgcg gcgtggccat gtacttggcg
     3181 gtggtcacgc ggctgatggt ggattcgtgc acaccca
//
LOCUS       DAOMVO010000282         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8795_length_3217_cov_14.028779, whole genome shotgun sequence.
ACCESSION   DAOMVO010000282 DAOMVO010000000
VERSION     DAOMVO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8795_length_3217_cov_14.028779"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PKV30_11475"
     CDS             <1..650
                     /locus_tag="PKV30_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRL30864.1"
                     /translation="LRERLLSADPTLKLLTAKQPLRVPKTLSEAQVEALLNAPDTDTP
                     LGLRDRTMLELMYASGLRVSELTTLKTFNVAFNEGVLRVMGKGSKERLVPFGEEAGDW
                     LQRYVSESRPALLGPRQTDDLFVTSAGRTPGTAMSRVMFWGLVKRYALQAGVTAPLSP
                     HTLRHAFATHLLNHGADLRAVQMLLGHADISTTTIYTHIARERLKDLHAQHHPRG"
     gene            1063..2049
                     /locus_tag="PKV30_11480"
     CDS             1063..2049
                     /locus_tag="PKV30_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL30865.1"
                     /translation="MNSTTFIANRRAALATLAALAASPAAFAQPWPAKPVKITVAYPP
                     GGVADSVARLLADRLAPVLGQSVVVDNKAGASGSIGMDAVAKAAPDGHSLGFAAISPL
                     VLNPHLGKSPFDPVKDIVPVVSVIASPVLLLGTSALAARDFKALIDAAKAKPGAVRWA
                     TSGPASLGHIVLEQVKAGTGADITHIPYKGGGQQINDALGGQFEVLSVNAGPAVMGHI
                     KAGKLTSLAVGSPARLEQLPNVPTLAELGLPAANVASVFGLFAPAGTPVAVIERLNAE
                     VNKLLKDKDVLAKIDAADNLPTGGSAADFGKFIANESANNARIIKAAGITAG"
     gene            2127..>3217
                     /locus_tag="PKV30_11485"
     CDS             2127..>3217
                     /locus_tag="PKV30_11485"
                     /inference="COORDINATES: protein motif:HMM:NF020322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL30866.1"
                     /translation="MNGTGLLSQPALQAALLAAAGALAYWLKDVPGLVVSWARRFFIS
                     TLTVDSRDEFLFSALVEYMDTHPRLRGVNQFTARSVRRGTAYQSLDDDLRNGQPPRAY
                     LSPGEGLHILRLDGRWLWIRRDVQVAQAVFEKISLSQFGRSPARLQAFLQAAIDTRAA
                     RETDTLSVYIPNPFHGGDWMRARLGSRRPLASVVLKAGQGEALLADLERFFDSRERYA
                     QLGIPWRRGYLLYGPPGTGKTSLVTALASELHLNVCTLSLASPIVTDEKIHTLLASVP
                     QRSLLLIEDVDAFFRERDAAHAQVKLSFSGFLNALDGVATQEGTVLFMTTNHVERLDP
                     ALIRAGRIDERVELGYADEEQLRRLYRKF"
BASE COUNT          513 a         1163 c         1060 g          481 t
ORIGIN      
        1 cgctgcgcga gcgcctgctg agcgccgacc cgacgctcaa actgctcacc gccaagcagc
       61 cgctgcgcgt gcccaagacg ctgagcgagg cgcaggtcga ggcgttgctg aacgcgcccg
      121 acaccgacac gcccctgggc ctgcgcgacc gcaccatgct ggagctgatg tacgccagcg
      181 gcctgcgtgt gagcgagctg accacgctca agaccttcaa cgtcgccttc aacgaaggcg
      241 tgctgcgcgt gatgggcaaa ggcagcaagg agcgcctggt gcccttcggc gaagaggcgg
      301 gcgactggct gcagcgctat gtttctgaaa gccgaccggc cctgctgggc ccgcgccaga
      361 ccgatgatct gttcgtcacc agcgccggcc gcacgcccgg cacggcgatg tcgcgcgtga
      421 tgttctgggg cctcgtcaag cgctacgcgc tgcaggccgg cgtgaccgcg ccgctgtcgc
      481 cccacacgct gcgtcacgcc ttcgccacgc acctgctcaa ccacggcgcc gacctgcgcg
      541 ccgtgcagat gctgctgggc cacgccgaca tcagcaccac caccatctac acccacatcg
      601 cgcgcgagcg gctgaaggat ttgcacgcgc agcaccatcc gcgcggttga gagtcttttt
      661 gggctgcagg cccttgtgca gcaagcgctg acagctatcg atatcgaagt atccacgggc
      721 ttgtgggagc ggccgtggcc gcgatggctg agccggatca agcgacgcgc tgccatcgcg
      781 gccaaggccg ctcccacaaa ggccggcata gcgtcgacgg ctgagatttc acgatcatgg
      841 atgcaaaaaa tagggagtat gtgatattga atcccgtgaa tagggcgcta gtggattgct
      901 ttatggggag tatgtggccc atgccctgat gcgggcgcgg gcttgcccgc gatgaccgct
      961 gcccgtggcc gacgcgcgcc acgcggccca ggcttccccc agcccccggc atccggccgc
     1021 ccaccgccgg gccgcgccac ccgctgtcgc acaatcgccg ccatgaactc caccaccttc
     1081 atcgccaacc gccgcgcggc cttggccacc ctcgccgcgc tcgccgcatc acccgccgcc
     1141 ttcgcccagc cctggcccgc caaacccgtc aagatcaccg tcgcctaccc gccgggcggc
     1201 gtggccgaca gcgtggcccg cctgctggcc gaccgcctgg cgcccgtgct gggccagagc
     1261 gtggtggtcg acaacaaggc cggcgccagc ggcagcatcg gcatggacgc cgtggccaag
     1321 gccgcgcccg atggccacag cctgggcttt gcggccatca gcccgctggt gctcaacccg
     1381 cacctgggca agtcgccgtt cgacccggtc aaggacatcg tgccggtggt cagcgtcatc
     1441 gcctcgcccg tgctgctgct gggcacctcg gcgctggcgg ccagggattt caaggcgctg
     1501 atcgacgccg ccaaggccaa gcccggcgcc gtgcgctggg cgacgtccgg ccccgcctcg
     1561 ctcggccaca tcgtgctgga gcaggtgaag gccggcaccg gcgccgacat cacccacatc
     1621 ccctacaagg gcggcggcca gcagatcaac gatgcgctgg gcggccagtt cgaggtgctg
     1681 agcgtgaacg ccggcccggc cgtgatgggc cacatcaagg ccggcaagct cacgtcgctg
     1741 gccgtgggct cgcccgcgcg gcttgagcag ttgccgaacg tgcccacgct ggccgagctg
     1801 ggcctgccgg cggccaacgt ggcctcggtg ttcggcctgt tcgcgcctgc cggcacgccg
     1861 gttgccgtga tagagcgcct caacgccgag gtcaacaaac tgctgaagga caaggacgtg
     1921 ctggccaaga tcgacgccgc cgacaacctg cccaccggcg gcagcgcggc cgacttcggc
     1981 aagttcatcg ccaacgaatc ggccaacaac gcgcgcatca tcaaggcggc gggcatcacg
     2041 gcgggttgag tttgatttca tattgatagc tggtggcgct tacccataaa gtgctgcagc
     2101 ccgatttgac ctagaaaaag gcactcatga acggcaccgg cctgctctcc caacccgccc
     2161 tgcaggccgc gctgctggcc gccgcaggcg cgttggcgta ttggctgaag gacgtgccgg
     2221 gcctggtcgt cagttgggcc aggcgcttct tcatcagcac gctcaccgtc gattcgcgcg
     2281 acgagttcct gttcagcgcg ctggtcgagt acatggacac gcaccccagg ctgcgcggcg
     2341 tcaaccagtt caccgcgcgc agcgtgcggc gcggcacggc ctaccagagc ctggacgacg
     2401 acctgcgcaa cggccagccg ccgcgcgcct acctgtcgcc cggcgagggc ctgcacatcc
     2461 tgcggctgga tgggcgctgg ttgtggatcc ggcgcgacgt gcaggtggcg caggccgtgt
     2521 tcgagaagat cagcctgtct cagttcggcc gctcgcccgc gcgcctgcag gcctttctgc
     2581 aggccgcgat cgacacccgc gccgcgcgcg agaccgacac gctctcggtc tacatcccca
     2641 acccgttcca cgggggcgat tggatgcgcg cgcgcctcgg ctcgcggcgg ccgctggcgt
     2701 cggtggtgct gaaagccggg cagggcgagg cgctgctggc cgacctggaa cgcttcttcg
     2761 acagccgcga gcgctatgcg caactgggca ttccgtggcg gcgcggctac ctgctgtacg
     2821 gcccgcccgg caccggcaag acctcgctgg tcaccgcgct ggcgagcgag ctgcacctga
     2881 acgtgtgcac cctcagcctg gccagcccca tcgtcaccga cgagaagatc cacacgctgc
     2941 tggcctcggt gccgcagcgc tcgctgctgc tgatcgagga cgtcgacgcc ttcttccgcg
     3001 aacgcgacgc cgcgcacgcg caggtcaagc tgtcgttcag cggcttcctc aacgcgctcg
     3061 acggcgtggc cacgcaggag ggcacggtgc tgttcatgac gaccaaccac gtcgagcggc
     3121 tcgacccggc gctcattcgc gccggccgca tcgacgagcg ggtggaactg ggctatgcgg
     3181 acgaagagca gttgcggcgg ctgtaccgca agttcca
//
LOCUS       DAOMVO010000283         3216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_8799_length_3216_cov_13.298640, whole genome shotgun sequence.
ACCESSION   DAOMVO010000283 DAOMVO010000000
VERSION     DAOMVO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8799_length_3216_cov_13.298640"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1290
                     /locus_tag="PKV30_11490"
     CDS             100..1290
                     /locus_tag="PKV30_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRL30867.1"
                     /translation="MSNNPRPLDGITVISLEHAIAAPFASRQLADLGARVIKVERPGA
                     GDFARAYDTRVKGEASHFIWTNRSKESLTLDLKHPDALAVLMDLLKDADVLLQNLAPG
                     AAARMGISWEALHEKHPKLIVCDISGYGADGPYRDKKAYDLLIQSEAGFLSITGTPET
                     PSKAGNSMADIAAGMYAFTSILSALLLRGKTGEGSHIDVSMLEALGEWMGYPMYYAFD
                     GAAPPPRTGASHASIYPYGPFPAGDGGTVMLGLQNEREWKVFCDVVLQDAALASDERF
                     NANAQRNANREALRALIVEAFSKLSTPQVEARLDQAQIANARMNDMAGLWAHPQLQAR
                     GRWRDVGSPAGAVPALLPPGRNSAFDHRMDAVPRVGEHTDAILLALGRSAEQVAALRA
                     AQAI"
     gene            1358..2731
                     /locus_tag="PKV30_11495"
     CDS             1358..2731
                     /locus_tag="PKV30_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRL30868.1"
                     /translation="MNTPPNLPAEQLAAFAAGLRFDQIPEPVVRFTENLLVDWFGSAV
                     AGHGSRPVESIARFAQSMGSAAGPSEVIFSRQTSSPYLAAMANAAASHLAEQDDVHNG
                     SVFHPATVVFSPAVAVAQALGASGQQLIAASVAGYEVGIRVGEFLGRSHYKVFHTTGT
                     AGTLAAAAAVGHLLGLNAEQMQHAFGSAGTQSAGLWEFLRTAADSKQLHTAHAAGAGL
                     MAAYLAQDGFTGAADIFGGPQGMAAGMSSDADAARLTDGLGTRWATAETSFKWHACCR
                     HTHPAADALLAVMQAHGLHAHDIARVVAHVHQGAIDVLGPVVDPRTVHQSKFSMGTAL
                     ALAAQHGHAGLNEFDAEFQSAATQALRDKVAMVLDEEVDTAYPARWIGKVSVTTTDGR
                     TLHGRVDEPKGDPGNTLSRDEITAKALRLAAWSGGASEMEMRQAVDALWQVADWPRVG
                     MLLKGTA"
     gene            2874..>3216
                     /locus_tag="PKV30_11500"
     CDS             2874..>3216
                     /locus_tag="PKV30_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRL30869.1"
                     /translation="MIAGAITFLFVPGNRPDRFAKALASGAGAVIVDWEDAVAPADKI
                     AVREQFAQAWPSVPPADRARLLLRINAAGTPWHAGDVAALPALAAQGLITVVVPKAES
                     AAALAPVAAALG"
BASE COUNT          533 a         1111 c         1093 g          479 t
ORIGIN      
        1 cctacttttc tttggcggag caaagaaaag taggtgcgcc gccgggcgca catcccggcc
       61 aacagcgcat ccaatccgcc tgaaggcagc tagcaaaaca tgagcaacaa cccaagaccc
      121 ctcgacggca tcaccgtcat ctccctcgaa cacgccatcg ccgccccctt cgccagccgc
      181 cagctggccg acctgggcgc gcgcgtcatc aaggtcgaac gccccggcgc gggcgacttc
      241 gcgcgtgctt acgacacccg cgtgaagggc gaggcctcgc acttcatctg gaccaaccgc
      301 agcaaggaaa gcctcacgct cgacttgaag caccccgacg cactcgccgt gctgatggac
      361 ctgctcaaag acgccgatgt gctgctgcaa aacctggcgc ccggcgcggc ggcgcgcatg
      421 ggtatctcat gggaggcgct gcacgaaaag caccccaagc tcatcgtctg cgacatctcc
      481 ggctacggcg ccgacggccc gtaccgcgac aaaaaggcgt acgacctgct gatccagagc
      541 gaggcgggtt ttctgtcgat caccggcacg cccgagacgc cgtcgaaggc cggcaactcg
      601 atggccgaca tcgccgccgg catgtacgcc ttcaccagca tcctgtcggc gctgctgctg
      661 cgcgggaaga cgggcgaggg cagccacatc gacgtgtcga tgctcgaagc gctgggcgag
      721 tggatgggct acccgatgta ctacgccttc gacggtgccg cgccgccgcc gcgcaccggc
      781 gcatcgcacg ccagcattta cccctatggg ccgtttccgg ccggcgatgg cggcaccgtg
      841 atgctgggcc tgcagaacga gcgcgagtgg aaggtgtttt gcgacgtggt actgcaagac
      901 gcggcgctgg cgagcgacga gcgcttcaat gccaatgcgc aacgcaacgc caaccgcgag
      961 gcgttgcgcg cgctgatcgt cgaggcgttt tcaaagctga gcacgcccca agtcgaggcg
     1021 cggctggacc aggcgcagat cgccaacgcg cgcatgaacg acatggccgg gctgtgggca
     1081 cacccgcagt tgcaggcgcg cggacgttgg cgcgacgtgg gctcgcccgc cggcgcggtg
     1141 cccgcgctgc tgccgccggg ccgcaacagc gcgttcgacc accgcatgga cgccgtgccg
     1201 cgcgtcggcg agcacaccga tgcgatcctg cttgcattgg ggcgcagtgc ggagcaggtc
     1261 gcggcgctgc gtgcagccca ggcgatctga cttgaactac ttcactattg atagctgttt
     1321 gcgcttgtct gacgtgcgcc agcggccgat ttgattcatg aatacaccgc ccaacctgcc
     1381 cgccgaacaa ctggccgcct tcgcagccgg cctgcgtttt gatcagattc ccgagcccgt
     1441 ggtgcgcttc accgagaacc tgctggtcga ctggttcggc tcggcggtgg cgggccatgg
     1501 ttcacgcccc gtcgagagca tcgcgcgctt cgcccagagc atggggtcgg ccgccggccc
     1561 gagcgaggtc atctttagcc gccagaccag cagcccgtac ctggccgcca tggccaacgc
     1621 cgccgcctcg cacctggccg agcaggacga cgtgcacaac ggctcggtgt ttcacccggc
     1681 gacggtggtg ttctcgcccg ccgtcgcggt ggcgcaggcg ttgggcgcga gcggccagca
     1741 attgatcgcg gccagcgtgg cgggctatga ggtgggcatt cgcgtcggcg aattcctcgg
     1801 ccgctcgcac tacaaggtgt tccacaccac gggcacggcc ggcacgctgg cggccgcggc
     1861 cgcggtgggt cacttgctgg gcctgaatgc cgaacaaatg caacacgcct tcggttcggc
     1921 cggcacacag tcggccgggc tgtgggaatt tctgcgcacc gccgccgaca gcaagcagtt
     1981 gcacaccgcg cacgccgccg gtgcgggcct gatggcggcg tacctggcgc aggacggctt
     2041 caccggtgcg gccgatatct tcggcggccc gcaaggcatg gccgccggca tgagcagcga
     2101 cgctgatgcg gcgcgcctga ccgatggctt gggcacgcgc tgggcgacgg ccgaaacctc
     2161 gttcaaatgg catgcctgct gccgccacac gcaccccgcc gccgatgcgc tgctggcggt
     2221 gatgcaggcg cacggcctgc atgcccatga catcgcgcgc gtcgtcgcgc acgtgcacca
     2281 gggcgccatc gacgtgctgg ggccggtggt cgatccgcgc accgtgcacc agtcgaagtt
     2341 cagcatgggc accgcgctgg cgctggcggc gcagcacggg cacgcgggcc tgaacgagtt
     2401 cgacgccgaa tttcaaagcg ccgccacgca ggcattacgc gacaaggtgg cgatggtgct
     2461 cgatgaagaa gtcgacaccg cctacccggc acgctggatc ggcaaggtca gcgtaaccac
     2521 cacggacggc cgcacgctgc atgggcgcgt ggacgagccc aagggcgacc ctgggaacac
     2581 gctgagccgc gacgagatca cggccaaggc gctgcgtctt gcggcatgga gcggcggcgc
     2641 cagcgagatg gagatgcgtc aggccgtcga tgcgctgtgg caggtggcgg attggcctcg
     2701 cgtgggaatg ctgctgaaag gtactgcatg aattgccgtg gtcgtgctcc gttttgcgcc
     2761 ctctcccgct ggcgggagag ggtgggggtg agggtggttt gcgcgacccg catcagcgct
     2821 gtcggccctc acctcgccct ctcagaggga gtaaagacaa ccaagagatt cacatgatcg
     2881 ccggcgccat caccttcctc ttcgtccccg gcaaccgccc cgaccgcttt gccaaggcgc
     2941 tggccagcgg cgccggcgcg gtgatcgtcg attgggaaga cgccgtggca ccggccgaca
     3001 agatcgccgt gcgcgagcag ttcgcgcaag cttggcccag cgtgccgcca gccgaccgcg
     3061 cgcgcctgct gctgcgcatc aacgccgccg gcacgccctg gcatgcgggc gacgtggccg
     3121 cgctgcccgc gctcgccgcg cagggcctga taacggtggt cgtgcccaag gccgaaagcg
     3181 ctgccgcact ggcgcccgtg gcggcggcgc tgggcg
//
LOCUS       DAOMVO010000284         3159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9047_length_3159_cov_17.743879, whole genome shotgun sequence.
ACCESSION   DAOMVO010000284 DAOMVO010000000
VERSION     DAOMVO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_3159_cov_17.743879"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKV30_11505"
     CDS             complement(<1..670)
                     /locus_tag="PKV30_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL30870.1"
                     /translation="MGKVTGFMEFERIEEGYAPVAERVKHYKEFVLALDDAKARQQGA
                     RCMDCGTPFCNSGCPVNNIIPDFNDLVYRGDWKNAFAVLDSTNNFPEFTGRICPAPCE
                     AACVLNINNDPVGIKSIEHAIIDRAWDEGWVVPRKPAHKTGKKVAVVGSGPAGLAAAQ
                     QLARAGHDVTLFEKNDRVGGLLRYGIPDFKLDKAHIDRRVEQMQAEGVTIRTGVLVGG
                     MPGEG"
     gene            complement(697..1170)
                     /locus_tag="PKV30_11510"
     CDS             complement(697..1170)
                     /locus_tag="PKV30_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRL30871.1"
                     /translation="MASYTADLLWERGEQDFLSNRYSRRHLIRFDGGAVLAGSSSPHV
                     VPQPWSDASAVDPEEAFVASLSSCHMLWFLSIAAKRGFCVDRYADAAEGVMARNAERR
                     MAMTVVTLRPTVAFSGAVQPTPEQVANMHHEAHDECFIANSVKTEVRCEPQMAAA"
     gene            complement(1183..>3159)
                     /locus_tag="PKV30_11515"
     CDS             complement(1183..>3159)
                     /locus_tag="PKV30_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRL30872.1"
                     /translation="GKSNTGEGGEDALRYRNELKGIPIKQGQTMSDLLGNIFEVDYPL
                     QDGDSLRSKIKQVASGRFGVTAEYLSSADQIQIKMAQGAKPGEGGQLPGGKVSDYIGK
                     LRHSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVAPHSDISVKLVSEVGVGTIAAGV
                     AKCKSDHVVIAGHDGGTGASPWSSIKHAGSPWEIGLAETQQTLVLNRLRGRIRVQADG
                     QMKTGRDVAIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFT
                     GKPEHVVNYFFFVAEEVRQIMAQLGIRKFDDLIGRSDLLDTRKGIAHWKAKGLDFSRL
                     FAQPDVPADVPRFHVATQDHGLEKSLDNRLIEKSRPAIDQGEAVKIMDTARNVNRSVG
                     AMLSGAVTKVHPEGLPDDTIRIQLEGTGGQSFGAFLCKGITLQLIGDANDYTGKGLSG
                     GRIAIRPSLDFRGNAADNIIVGNTVMYGATTGEAYFSGVAGERFGVRLSGATAVVEGT
                     GDHGCEYMTGGTVAVLGKTGRNFAAGMSGGVAYVYDEDGQFAKRCNLSMVTLEKVLPA
                     AEQKAAQDVGTWHRGQTDEEQLKKLLDDHLRWTGSRRARELLDNWATAREKFVKVFPT
                     EYKRALGEIHVKKQALAQTSQARAATKKEATPAK"
BASE COUNT          465 a         1041 c         1034 g          619 t
ORIGIN      
        1 ggccttcgcc gggcatgccg ccgaccagca ccccggtgcg gatcgtcacg ccctcagcct
       61 gcatctgctc cacgcggcgg tcgatgtggg ccttgtcgag cttgaagtcg ggaatgccgt
      121 agcgcagcag gccgccgacg cggtcgttct tttcgaacag cgtcacgtcg tggccggcgc
      181 gtgccagctg ctgcgcggcc gccagcccgg cggggccgga gccgaccacg gccaccttct
      241 tgccggtctt gtgcgcgggc ttcctgggca ccacccagcc ttcgtcccac gcgcggtcga
      301 tgatggcgtg ctcgatgctc ttgatgccga ccggatcgtt gttgatgttc agcacgcagg
      361 ccgcctcgca cggcgcgggg cagatgcggc cggtgaactc ggggaagtta ttggtcgaat
      421 cgagcaccgc aaaggcgttc ttccagtcgc cgcgatacac cagatcattg aaatccggaa
      481 tgatgttgtt gaccgggcag cccgaattgc agaacggcgt gccgcagtcc atgcagcgcg
      541 cgccctgctg gcgcgccttg gcgtcatcga gcgcgaggac gaattccttg tagtgcttca
      601 cgcgctcggc cacgggcgcg tagccctctt cgatgcgctc gaattccatg aagccggtga
      661 cttttcccat gatgcttttc tctctacttt gatcgttcag gctgcggcca tctgcggctc
      721 gcagcgcact tcggtcttga ccgagttggc gatgaaacat tcgtcatgcg cctcgtggtg
      781 catgttggca acttgctcgg gtgtgggttg cacggcgcca gaaaacgcga ccgtcggtcg
      841 cagcgtgacg accgtcatcg ccatgcggcg ctcggcgttg cgcgccatca cgccctcggc
      901 cgcgtcggcg tagcggtcca cacaaaagcc gcgcttggcg gcaatcgaca gaaaccacag
      961 catgtggcag ctggacagcg atgcgacgaa ggcttcttcc gggtcgaccg ccgacgcgtc
     1021 cgaccacggc tggggcacga cgtgcggcga cgacgagccg gccagcaccg cgccgccgtc
     1081 aaagcggatc aggtggcggc ggctgtagcg gttggacaga aagtcctgtt cgccacgttc
     1141 ccacagcaga tcggcggtgt agctggccat gcgctgttcg actcacttgg cgggagttgc
     1201 ttctttttta gtagctgctc gcgcttgact ggtctgcgcc agagcctgtt ttttgacatg
     1261 aatctcaccc agtgcgcgct tgtactcggt cgggaagacc ttgacgaact tctcacgcgc
     1321 ggtggcccag ttgtcgagca gttcgcgcgc gcggcggctg ccggtccagc gcaagtggtc
     1381 gtcgagcagc ttcttgagtt gctcttcgtc ggtctggccg cggtgccaag tgcccacgtc
     1441 ttgcgcagcc ttttgctcgg cggccggcag cactttttcc agcgtcacca tcgacaggtt
     1501 gcagcgcttg gcgaactggc cgtcttcgtc gtagacgtag gccacgccgc ccgacatgcc
     1561 ggcagcgaag ttgcggccgg tcttgccgag cacagccacg gtgccgccgg tcatgtattc
     1621 gcagccgtga tcgcctgtgc cttcgacgac agccgtggcg cccgacaggc gcacgccgaa
     1681 gcgctcgccg gccacgcccg agaaatacgc ctcgcccgtc gtcgcgccgt acatcacggt
     1741 gttgccgacg atgatgttgt cggccgcgtt gccgcggaag tccaggctgg gccggatggc
     1801 gatgcgcccg cccgacaggc ccttgccggt gtagtcgttg gcgtcgccga tcagctgcag
     1861 cgtgatgccc ttgcacagga aggcgccgaa cgactggccg cccgtgccct cgagctgaat
     1921 gcggatggtg tcgtcgggca ggccttcggg gtgcaccttg gtcaccgcgc ccgacagcat
     1981 ggcgccgacc gagcggttga cgttgcgcgc cgtgtccatg atcttgacag cctcgccctg
     2041 gtcgatggcg gggcgggact tctcgatcag gcggttgtcg agcgatttct ccaacccgtg
     2101 gtcctgcgtg gcgacgtgga agcgcggcac atcggccggc acgtccggct gcgcgaacag
     2161 gcggctgaag tccaagccct tggccttcca atgcgcgata cctttgcgcg tgtcgagcag
     2221 gtcgctgcgg ccgatcaaat catcgaactt gcggatgccc agctgcgcca tgatctggcg
     2281 cacttcttct gctacgaaaa agaagtaatt aaccacgtgc tcgggcttgc cggtgaactt
     2341 cgcgcgcagc accgggtctt gcgtggccac gcccaccggg caggtattga ggtggcactt
     2401 gcgcatcatg atgcagcctt cgaccaccag cggcgcggtg gcgaagccga attcgtcggc
     2461 gcccagcagc gcgccgatgg cgacgtcgcg gccggtcttc atctggccgt cggcctgcac
     2521 gcggatgcgg ccacgcaggc ggttcagcac cagcgtctgc tgcgtttcgg ccaggccgat
     2581 ttcccacggc gagccggcgt gcttgatcga cgaccaaggc gatgcgcccg tgccgccgtc
     2641 gtgccctgca atcaccacgt ggtcgctctt gcacttggcc acgcccgcgg cgatggtgcc
     2701 cacgcccact tcgctgacca gcttgacgct gatgtcgctg tgcggcgcca cgttcttcaa
     2761 atcgtggatc agctgcgcca ggtcttcgat cgaatagatg tcgtggtgcg gcggcgggct
     2821 gatgaggccc acgccgggca cgctgtggcg cagcttgccg atgtagtcgc tgaccttgcc
     2881 gcccggcagc tggccgccct cgcccggctt ggcgccctgc gccatcttga tctgaatctg
     2941 gtcggcgctg ctcaggtatt cggcagtgac gccaaagcgc cccgaggcca cctgcttgat
     3001 cttgctgcgc aggctgtcgc cgtcttgcag cgggtaatcg acctcgaaga tgttgcccag
     3061 caggtcggac atggtctggc cctgcttgat ggggatgcct ttaagctcat tgcggtagcg
     3121 cagcgcgtct tcgccgccct cacccgtgtt gctcttgcc
//
LOCUS       DAOMVO010000285         3136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9132_length_3136_cov_13.525154, whole genome shotgun sequence.
ACCESSION   DAOMVO010000285 DAOMVO010000000
VERSION     DAOMVO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9132_length_3136_cov_13.525154"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..391
                     /locus_tag="PKV30_11520"
     CDS             23..391
                     /locus_tag="PKV30_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HRL30873.1"
                     /translation="MQARAPIPILRLFDEAAARAFYVDFLGFRVDWEHRFEADLPLYM
                     QLTLGDCVLHLSGHHGDATPGSHVRVPCTGLREFQQALLAKAYRHARPGVEAQPWGLD
                     MTVSDPFGNRLTFTQALNAA"
     gene            complement(404..886)
                     /locus_tag="PKV30_11525"
     CDS             complement(404..886)
                     /locus_tag="PKV30_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRL30874.1"
                     /translation="MRTNTHDIHHQIAIRAPRSAVYRAIAEPAQIGRWWGAQTPVPTP
                     EGLVLEHQAGPYGTVRLRVVDLQPDARIEWACIGDYPPDNPASAWVGTRFVFELSIGE
                     SGAAMVERACSPAPIAELTTVDFRQTDYDLRGRFAGFNNSAWGQVLQSLKAACEAKAA
                     "
     gene            complement(883..1224)
                     /locus_tag="PKV30_11530"
     CDS             complement(883..1224)
                     /locus_tag="PKV30_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation protein"
                     /protein_id="HRL30875.1"
                     /translation="MTKYLISFPAAAMVVPDADWEAVVRDSHAVIDEAKAAGVYVFGG
                     GIDESVATALVSADGSAAEGGYPWAPRLNGGFTVLELPSREAAVAWAARIAKACRCDQ
                     ELRAFGFDPQS"
     gene            complement(1221..1646)
                     /locus_tag="PKV30_11535"
     CDS             complement(1221..1646)
                     /locus_tag="PKV30_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRL30876.1"
                     /translation="MNITIETTVNAPIDRVWAAWSDPRAIEQWNAASHDWHTPRASVD
                     LREGGQFSSRMEAKDGSMGFDFAGTYTRIEPQRLIEYAMGDTRTVRVEFIPAGDAVTV
                     RETFDAEDTHSAEQQRQGWQAILDNFARHVEVSSGGTMA"
     gene            complement(1737..2174)
                     /locus_tag="PKV30_11540"
     CDS             complement(1737..2174)
                     /locus_tag="PKV30_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL30877.1"
                     /translation="MQFAPYLNFNGNCAEAFAFYKDLFKGQIVHQSTFGEMPPDDKMP
                     PLPPEAKDGIMHVHLQVGEQALMGSDAMPGAGEACAGGYVKPQGLWVSIGVADTAEGQ
                     RVFKALADGGQVAMPFEKTFWSEGFGMVTDRFGTPWMVNVAHK"
     gene            complement(2205..2708)
                     /locus_tag="PKV30_11545"
     CDS             complement(2205..2708)
                     /locus_tag="PKV30_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRL30878.1"
                     /translation="MPSVQARIQPCLWFDTQAEEAVRFYTGVFADSRIGAITHYPEAG
                     REQHGKPPGSVMTVAFELLGQPMLALNGGPHFKFSPALSLMVLCDTQDEIDHYWNALS
                     QGGDPAAQECGWLKDRYGLSWQITPTIFQEMEASTDRPAVERYMAVLMTMKKLDIARL
                     RQAFDGR"
     gene            complement(2718..3071)
                     /locus_tag="PKV30_11550"
     CDS             complement(2718..3071)
                     /locus_tag="PKV30_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRL30879.1"
                     /translation="MKPDTYVDGFVCAVPTARREEYRQHAINAARAFKKRGALSVVDC
                     WGNDVPEGKLTSFSLAVQRKPDETVVFSWVTWPSKQVRDEAWKLLMDDPDMPKDMPFD
                     GQRLIYGGFELLSDV"
BASE COUNT          491 a         1034 c         1042 g          569 t
ORIGIN      
        1 cgcggcgcca gcgcgggatg tgatgcaagc ccgggcgccc atcccgatcc tgcgcctgtt
       61 cgatgaagca gcggcgcgcg cgttctatgt cgactttctc ggctttcgcg tcgattggga
      121 acatcggttt gaagccgatc tgccgctgta catgcagctg acgctgggcg actgcgtgct
      181 gcacctgtcc ggccaccacg gcgacgccac gcccggcagc cacgtgcgcg tgccgtgcac
      241 cgggctgcgc gagtttcagc aggcgctgct cgccaaggcg taccgccacg cccgccccgg
      301 cgtcgaggcg cagccgtggg gcctggacat gacggtgagc gatccgtttg gcaatcggct
      361 gacgtttacg caggcgttga acgccgcgtg agccgctggc atttcaagcc gcttttgcct
      421 cgcacgccgc cttcagcgac tgcaacacct gcccccaggc gctgttgttg aagccggcaa
      481 agcgcccgcg caggtcgtag tcggtctggc gaaagtccac tgtggtcagc tcggcgatag
      541 gcgccggact gcaggcgcgc tcgaccatcg ccgcgccgct ctcgccgatg gacaactcaa
      601 agacaaagcg cgtgcccacc caggcggagg cggggttgtc cggcgggtag tcgccgatgc
      661 aggcccactc gatgcgcgcg tccggctgca gatcgaccac gcgcagccga accgtgccgt
      721 agggcccggc ctggtgttcc agcaccagcc cctcgggcgt gggcaccggc gtttgcgcgc
      781 cccaccagcg gccgatttgc gccggctcgg cgatggcgcg gtacaccgcg ctgcgcggcg
      841 cgcggatggc gatctggtgg tggatgtcat gggtgttcgt tctcaagact gcggatcgaa
      901 gccgaaggcg cgcagttcct gatcgcaacg acaggctttg gcgatgcgcg ccgcccacgc
      961 gaccgcggcc tcgcgcgatg gcagttcgag cacggtaaaa ccgccgttga gccgcggcgc
     1021 ccatgggtag ccgccctcgg cggccgaacc atcggccgag acgagcgcgg tcgcaacgct
     1081 ttcgtcgatg ccgccaccga agacatagac gccggccgcc ttggcctcgt cgatcacggc
     1141 gtgcgaatcg cgcaccaccg cttcccagtc ggcgtcgggc acgaccatcg cggcggcggg
     1201 gaaggagatc aggtacttgg tcatgccatc gtgccgcccg acgagacttc gacatgccga
     1261 gcaaagttgt ccaggatcgc ttgccagccc tggcgctgct gctcggcgct gtgcgtgtcc
     1321 tcggcgtcga aggtttcgcg caccgtcacg gcatcgcccg cggggatgaa ctcgacgcgc
     1381 acggtgcgcg tgtcgcccat ggcgtattcg atcaggcgct gcggctcaat gcgcgtgtag
     1441 gtgcccgcga agtcgaagcc catgctgccg tccttcgctt ccatgcgcga gctgaactgg
     1501 ccgccttcgc gcaagtccac gctggcgcgc ggcgtgtgcc agtcgtgcga ggcggcgttc
     1561 cactgctcga tggcgcgcgg gtcgctccag gccgcccaca cgcggtcgat gggggcgttg
     1621 acggtggttt cgatggtgat gttcatcgtg cgttgtccaa caatacacta cttaattaat
     1681 agctgctcgc gcttgtcccg caagcgctgg aggctgattt ggcttgaaaa aacgccttac
     1741 ttgtgcgcca cgttcaccat ccacggcgtg ccgaagcggt ccgtcaccat gccgaagcct
     1801 tcggaccaga aggtcttctc gaacggcatg gcgacctggc caccatcggc cagcgccttg
     1861 aagacgcgct gcccttcggc cgtgtcggcg acgccgatcg acacccacag gccttgcggc
     1921 ttgacgtagc cgccggcgca ggcctcgccc gcgccgggca tggcgtcgga gcccatcagc
     1981 gcctgctcgc cgacctgcag gtgcacgtgc atgatgccgt ccttcgcttc gggcggcagc
     2041 ggcggcatct tgtcgtcggg cggcatctcg ccaaaggtgc tctggtgcac gatctggccc
     2101 ttgaacaagt ctttgtagaa ggcaaacgct tcggcgcagt tgccgttgaa gttgagatac
     2161 ggggcgaatt gcataggggt ctccttggcg ggttgtgagc ggcttcagcg gccgtcgaaa
     2221 gcctggcgca ggcgcgcgat gtcgagcttt ttcatcgtca tcaacactgc catgtagcgc
     2281 tccacggcgg ggcggtcggt cgatgcttcc atctcctgaa agatcgttgg cgtgatctgc
     2341 cacgacaggc catagcgatc cttcagccag ccgcattctt gcgccgccgg atcaccgcct
     2401 tgtgacaagg cgttccagta atggtcgatc tcgtcctgcg tgtcgcacaa caccatcagc
     2461 gacagcgcag ggctgaactt gaagtgaggc ccgccgttga gcgccagcat gggctggccg
     2521 agcaactcga acgccaccgt catcacgctg cccggcggct tgccgtgctg ctcgcgcccg
     2581 gcctcggggt agtgcgtgat ggcgccgatg cgcgagtcgg cgaagacgcc ggtgtaaaag
     2641 cgcacggctt cctcggcctg ggtgtcgaac cacaggcagg gctggatgcg ggcttgaacg
     2701 ctgggcatgg cggtgcctta cacgtcggac agcagctcga agccgccgta gatcagccgc
     2761 tggccgtcga acggcatgtc cttgggcatg tcggggtcgt ccatcagcag tttccaggct
     2821 tcgtcgcgca cctgcttcga cggccaggtg acccacgaga agaccaccgt ttcgtccggc
     2881 ttgcgctgca cggcgagcga gaacgaagtg agcttgcctt cgggcacgtc gttgccccag
     2941 cagtcgacca cgctgagcgc gccgcgcttc ttgaaggcgc gcgcggcatt gatggcgtgc
     3001 tggcggtact cctcgcgccg ggcggtaggc acggcgcaga caaatccatc cacgtaggtg
     3061 tcaggtttca tgaaatctcc ttgtttcacg gtagggcgtt gtgatgtgta caacgtccac
     3121 atcattctgc cctagg
//
LOCUS       DAOMVO010000286         3129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9153_length_3129_cov_17.770332, whole genome shotgun sequence.
ACCESSION   DAOMVO010000286 DAOMVO010000000
VERSION     DAOMVO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_3129_cov_17.770332"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="hprK"
                     /locus_tag="PKV30_11555"
     CDS             complement(<1..882)
                     /gene="hprK"
                     /locus_tag="PKV30_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRL30880.1"
                     /translation="MKPTVISADVLFEDHRATLRWHWIAGLEASERRFDDAAISQARS
                     SADLVGYLNYIHPYRVQILGEREVAYLTNGSREDCARRVARIVTLEPPVLVLCDGQTA
                     PDALLSMCERAQIPMFATHESAAFVIDVLRAYLSKHFANRTTMHGVFMDILGLGVLIT
                     GESGLGKSELGLELISRGHGLVADDAVDLFRINQATIEGRCPELLQNLLEVRGIGLLD
                     IRAIFGETAVRRHMRLRLIVHLVRRETMERDYERLPYEPLTQEVLGVPIQKAVIQVVA
                     GRNIAVLVEAAVRNHILQ"
     gene            complement(926..1396)
                     /locus_tag="PKV30_11560"
     CDS             complement(926..1396)
                     /locus_tag="PKV30_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRL30881.1"
                     /translation="MNRLASILPESQVLVGVEATSKKRAFEEAGLLFENLHGLSRALI
                     TDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAVLQLAQPIGFDAPDEQPVGLLIF
                     LLVPEAATQKHLEILSEIAELLSDSGLRERIKAAGSVPELHGLIAQWQSAQVAP"
     gene            complement(1608..1937)
                     /gene="raiA"
                     /locus_tag="PKV30_11565"
     CDS             complement(1608..1937)
                     /gene="raiA"
                     /locus_tag="PKV30_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRL30882.1"
                     /translation="MNLTISGHHLDVTPALRSYVTGKLDRINRHFDQVVDVKVLLSVE
                     NQKEKEKRQRAECRIGVKGNDLFAESSHEDLYAAVDELVDKLDRQIVKHKDKLQSHDR
                     AAAKHLM"
     gene            2323..>3129
                     /locus_tag="PKV30_11570"
     CDS             2323..>3129
                     /locus_tag="PKV30_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRL30883.1"
                     /translation="MLNVFSLSNGRLFQEEIESLEELARFRPIWVDLEEPTLEEKRWV
                     KQYYGLSIPEDAIDDDIEESARFYEEDNGELHIRSDFLLIDTDDEARQVRVAFILNSA
                     NDQLRSHGVLFSIHDETVPAFRLLRMRARRMPGLLGDAKDVLLALLDGDAEYCADTLE
                     GIYDELEAAGKKVLDDNVTDEVAADVLATIARQEDMNGRIRRNVMDTRRAVSFLMRSR
                     MLDAEQFEEARQIQRDLDSLDSHTTFLFDKINFLMDATVGFININQNKIIK"
BASE COUNT          541 a         1033 c         1027 g          528 t
ORIGIN      
        1 ctgcaggatg tggttgcgca ccgccgcctc caccagcacg gcgatgttgc ggccggccac
       61 cacctggatc accgccttct ggatcggcac gcccagcacc tcctgcgtca gcggctcgta
      121 gggcagccgc tcgtagtcgc gctccatggt ttcgcgccgc accaggtgca cgatcaggcg
      181 cagccgcatg tggcggcgca ccgcggtctc gccgaagatg gcgcggatgt cgagcaggcc
      241 gatgccgcgc acttccagca ggttctgcag cagctcgggg cagcggccct cgatggtggc
      301 ctggttgatg cggaacaggt ccaccgcgtc gtcggccacc aggccgtggc cgcgcgagat
      361 cagctccagc cccagctcgc tcttgcccag gcccgactcg ccggtgatca gcacgcccag
      421 gcccaggatg tccatgaaca cgccgtgcat ggtggtgcgg ttggcgaagt gcttggacag
      481 gtaggcgcgc agcacgtcga tgacgaaggc cgccgattcg tgcgtggcga acatcgggat
      541 ctgcgcccgc tcgcacatcg acagcagcgc gtccggcgcg gtctggccgt cgcacagcac
      601 cagcaccggc ggctccagcg tgacgatgcg cgccacccgg cgcgcgcagt cctcgcgcga
      661 gccgttggtc aggtaggcga cctcgcgctc gcccaggatc tgcacgcggt aggggtggat
      721 gtagttcagg tagccgacca ggtcggcact ggagcgcgcc tggctgatgg cggcatcgtc
      781 gaagcggcgc tcggaggcct cgagcccggc aatccagtgc cagcgcagcg tggcgcggtg
      841 gtcctcgaac aggacgtcgg cactgatgac ggtgggcttc atggccggcg ctgcgtcgcg
      901 tcagcggcgc gggcagggcc cgcggtcacg gggcaacctg ggccgactgc cactgcgcga
      961 tcaggccgtg cagctcgggc accgagcccg ccgccttgat gcgctcgcgc aggcccgagt
     1021 cgctcagcag ctcggcgatc tcggacagga tctcgaggtg cttctgcgtc gccgcctcgg
     1081 gcaccagcag gaagatcagc aggcccacgg gctgctcgtc gggcgcgtcg aagccgatcg
     1141 gctgcgccag ttgcagcacc gcggccatgg gcgccttcag gcccttgatg cggccgtgcg
     1201 gaatggccac gccgtggccc aggccggtgg agcccaggcg ctcgcgcgcg aacaggctgt
     1261 cggtgatgag cgcgcgcgac aggccgtgca ggttctcgaa cagcaggccc gcttcttcaa
     1321 aagcgcgctt cttgctggtg gcttcgacac ccaccagcac ctgcgattca ggaaggatgg
     1381 aggctagacg gttcatgggc attgaccctg caattatgcg cctgtggcgc gcggcatttc
     1441 atggcggcgc cgccaccgcg tgcaggcagg catcgcccag ggggcgagtt cgggcacttc
     1501 accgggtacg tgcacatctc ggactgaaaa agaaagccgc ccggcaggat gccggggcgg
     1561 cttgcaaact ggagtctaca gtcttcgctc gttgttgtta cacgatacta catcaaatgc
     1621 ttggcagccg cacggtcgtg cgactgcagt ttgtccttgt gcttgacgat ttgccggtcc
     1681 agcttgtcga ccagttcatc gacggcggca taaaggtctt cgtggctgct ttcggcaaac
     1741 aggtcgttgc ccttgacgcc gatgcggcat tccgcgcgtt gccgtttctc cttttccttc
     1801 tggttttcga ccgaaagcag caccttgacg tccaccacct ggtcgaaatg ccggttgatc
     1861 cggtccagtt tgcccgtcac gtagctgcgc agggcagggg tgacatcgag gtggtgtccg
     1921 ctgatcgtca ggttcatgaa tagcctcctt ttggcataaa tgaacggccg gcccgggcgc
     1981 aggtgccctg ccgccaccac gacagcgccc gaaccaagtg tcttcacgct gtcatgcgag
     2041 tccactatgc cgccgtttcg cggcactggc aacccggggg ccaaagcccg cgcgtcccac
     2101 tgggtcgggg gccggcaggt tgacagggcg cgggttctgg cgctgcgggc caaagggcca
     2161 gtgccataat cgccacgtca catttttgtt gccgaaagcc cgccttggaa cccgtcccga
     2221 gtcgatagct ttctcctccc tgggacgctg gcgcgcgggg ggctgaaagc caccgcccca
     2281 cccgccgcgc cgccacaagc cttcaccagc ccagggaaac tcatgctcaa cgtcttctcg
     2341 ctgtccaacg gacggctgtt tcaggaagag atcgaatcgc tcgaagagct ggcgcgcttt
     2401 cggccgatct gggtcgatct ggaagaaccc acgctggaag aaaagcgctg ggtcaagcag
     2461 tattacggcc tgtccatccc cgaggatgcg atcgacgacg acatcgagga atcggcccgc
     2521 ttctacgaag aagacaacgg cgaactgcac attcgcagcg acttcctgct gatcgacacc
     2581 gacgatgaag cccgccaggt gcgcgtggcc ttcatcctga acagcgccaa cgaccagctg
     2641 cgcagccacg gcgtgctgtt ctcgatccac gacgagacgg tgccggcgtt tcgcctgctg
     2701 cgcatgcgcg cgcgccgcat gcccggcctg ctgggcgacg ccaaggacgt gctgctggcg
     2761 ctgctggacg gcgatgccga atactgcgca gacacgctcg aaggcattta cgacgaactg
     2821 gaagcggccg gcaaaaaggt gctggacgac aacgtgaccg acgaggtggc cgccgacgtg
     2881 ctggccacca tcgcgcggca ggaagacatg aacggccgca tccggcgcaa cgtgatggac
     2941 acgcgccgcg cggtcagctt tctgatgcgc agccgcatgc tggacgccga gcagttcgag
     3001 gaggcgcgcc agatccagcg cgacctggat tcgctggaca gccacaccac cttcttgttc
     3061 gacaagatca acttcttgat ggacgccacg gtcggtttca tcaacatcaa ccagaacaag
     3121 atcatcaag
//
LOCUS       DAOMVO010000287         3122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9176_length_3122_cov_12.357679, whole genome shotgun sequence.
ACCESSION   DAOMVO010000287 DAOMVO010000000
VERSION     DAOMVO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_3122_cov_12.357679"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..882
                     /locus_tag="PKV30_11575"
     CDS             136..882
                     /locus_tag="PKV30_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL30884.1"
                     /translation="MPATPAPKQSGVALVIGAGDSTGGAIAKRFAREGYVACVTRRSA
                     DKLQPLVDEIKAAGGDARGFASDARKEEDVVALIEQIEREVGPIEVLVFNIGANVPCS
                     ILEETARKYFKIWEMACFSGFLNAREVAKRQVARGRGTILFTGATAGTRGAAGFAAFA
                     GAKHALRALAQSMARELGPRGIHVGHVVVDGAIDTEFIRTQFPERYALKSQDGILNPD
                     HIAENYWYLHSQPRDAWTFELDLRPYMETW"
     gene            959..1555
                     /locus_tag="PKV30_11580"
     CDS             959..1555
                     /locus_tag="PKV30_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRL30885.1"
                     /translation="MTSQVEFFFDVGSPASYLAWTQLPKLCADAGAELKLRPMLLGGV
                     FQATGNASPVTVPAKGAYTFIDFARHAKRYGVTLIRNPHFPINTLMLMRAITGAQMHA
                     SERDAVLIDAIFKAIWTEQLNMNDPEVASVALANAGFPAGLIASWIGDAAVKARLKET
                     TDEAIARGVFGAPTMFVGDEMFFGQDRLEWVREALGAA"
     gene            complement(1560..1751)
                     /locus_tag="PKV30_11585"
     CDS             complement(1560..1751)
                     /locus_tag="PKV30_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30886.1"
                     /translation="MSVLTDVLDRLTDITTLRAQVTELIGQQREMRGVMLTQQKELAE
                     LRGQLKALIQMQSASAKKA"
     gene            complement(1748..2032)
                     /locus_tag="PKV30_11590"
     CDS             complement(1748..2032)
                     /locus_tag="PKV30_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRL30887.1"
                     /translation="MTSMVLTQVYLEPQQKKALTAQARQSGRRVSELMREAVDAAIAG
                     VTTEDLRVLDEGTRRAQGDLKAMLAELQDNASEHAAFMREIAKLQKAAAK"
     gene            complement(2119..2568)
                     /locus_tag="PKV30_11595"
     CDS             complement(2119..2568)
                     /locus_tag="PKV30_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30888.1"
                     /translation="MVEFQDKKIRTGVRLDHAPPGADDCDGWNPIAARLVSDEGRDPL
                     AHLLAAEAQHQQQAQADAAISLAAAWLRLIQHFDQDMADMPRHLLISRSWAYRCVQRA
                     SELARCQRHLSGALVDTSLPRPWRRFKLCRVPVQLTLDFGDHALLPL"
     gene            complement(2783..>3122)
                     /locus_tag="PKV30_11600"
     CDS             complement(2783..>3122)
                     /locus_tag="PKV30_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HRL30889.1"
                     /translation="YCLGLHAKMPPIEDQLPLMRALWPGPLVCRWNLHRRHGAYGYEG
                     AKAQYEPFDKLVDPDVETRQHLARVIAGTTGAGELAYVTVNNKAEGSAPLSVAALAEQ
                     LVGRATPDAA"
BASE COUNT          528 a         1006 c         1046 g          542 t
ORIGIN      
        1 ttcggcgtgg cggatgatca tgatcttgtg gggcatggtc gtctgtgctt gtcgaacagg
       61 ttgaacgcgc gggcgaaaac tttgcggcca ctctaaactg ggctaccgca aaagcccaca
      121 cggaggagac cgatcatgcc agccacccca gcgcccaagc agtccggcgt tgcccttgtc
      181 atcggcgccg gcgattcgac cggcggcgcc atcgccaaac gctttgcgcg cgaaggctac
      241 gtcgcctgcg tcacgcgccg ctcggccgac aagctccagc cgctggtgga cgaaatcaag
      301 gcggccggcg gcgacgcgcg cggctttgcc agcgatgcgc gcaaggaaga agatgtggtg
      361 gcgctcatcg agcagatcga gcgcgaggtg ggccccatcg aggtgctggt gttcaacatc
      421 ggcgccaacg tgccgtgcag catcctggaa gagacggcgc gcaagtactt caagatctgg
      481 gaaatggcgt gtttcagcgg cttcctgaac gcgcgcgaag tcgccaagcg gcaggtggcg
      541 cgcgggcgcg gcacgattct gttcaccggc gccacggcag gcacgcgcgg cgcggcgggg
      601 tttgcggcct ttgccggcgc caagcacgcg cttcgcgcgc tggcgcaaag catggcgcgt
      661 gagttggggc cgcgcggcat ccacgtcggg cacgtggtgg tcgatggcgc catcgacacc
      721 gagttcatcc gcacgcagtt ccccgagcgc tatgcgttga aaagccagga cggcatcctc
      781 aaccccgatc acatcgccga gaactactgg tacctgcaca gccagccgcg cgacgcgtgg
      841 acgttcgagc tggatctgcg gccgtacatg gaaacctggt aactccatat ttgatagctg
      901 ctcgcgcttg atggacaagc gctggcggca cttttcatat gaattctgga ggatgatcat
      961 gacaagccaa gtcgaattct tctttgacgt cggcagcccg gcgtcgtacc ttgcgtggac
     1021 gcaactgccc aagctgtgtg ccgacgccgg cgctgaattg aagctgcgcc ccatgctgct
     1081 gggcggcgtg ttccaggcca ccggcaatgc atcgcccgtc acggtgccgg ccaagggcgc
     1141 ctacaccttc atcgacttcg cgcggcacgc caagcgctat ggcgtcacgc tgatccgcaa
     1201 cccgcatttc ccgatcaaca cactgatgtt gatgcgcgcc atcaccggcg cgcaaatgca
     1261 cgcgtccgag cgcgacgcgg tgctgatcga cgccatcttc aaggccatct ggaccgagca
     1321 gctgaacatg aacgacccgg aagtcgcctc cgtcgcgctc gccaacgccg gctttccggc
     1381 cggcctgatc gcatcgtgga tcggcgacgc ggccgtgaag gcgcgcctga aggaaaccac
     1441 cgacgaagcc atcgcacgcg gcgtgttcgg cgcgccgacc atgttcgttg gcgacgagat
     1501 gttctttggg caggatcggt tggagtgggt gcgggaggcg ttgggggccg cgtgaaggtt
     1561 caggccttct tggctgacgc cgactgcatc tgaatcagcg ccttgagctg accgcgcagc
     1621 tcggccagct ctttttgctg cgtcagcatc acgccgcgca tttcgcgctg ctggccaatc
     1681 agctcggtga cctgggcgcg cagggtggtg atgtcggtca ggcggtccag gacatcggtc
     1741 agcacgctca tttggcggcg gccttttgca gcttggcgat ctcgcgcatg aaggcagcgt
     1801 gctcgctggc gttgtcctgc aactcggcca acatggcttt taggtcgccc tgggcgcggc
     1861 gcgtgccttc gtcgagcacg cgcaggtctt ccgtagtgac gccagcaatc gccgcgtcca
     1921 cggcttcgcg catcagctcg gacaccctgc ggccagactg cctggcctgc gcggtcagcg
     1981 ccttcttctg ctgcggctcc agatagactt gagtgaggac catcgaggtc atgacaggct
     2041 ccttatacgg gcgcgcgcca agcggcacgc gatgcatgtc aatatatatc atcatgcatc
     2101 ccgaatgcaa gactgaggtc acaacggcag cagcgcgtgg tccccgaaat ccagcgtcag
     2161 ttgcaccggc acgcggcaca gcttgaagcg tcgccacggg cgtggcaacg aggtgtcgac
     2221 cagcgcgccc gacaggtggc gctggcagcg ggccagctcg gatgcacgct gcacgcagcg
     2281 atacgcccat gagcgcgaaa tcagcaggtg tcgcggcatg tcagccatgt cctggtcgaa
     2341 gtgctggatg aggcgcagcc aggcggcggc caggctgatg gcggcgtcgg cttgcgcctg
     2401 ctgctgatgc tgcgcctcgg cggccagcag gtgcgccagc gggtcgcgac cctcgtcgct
     2461 gaccaagcgg gccgcaatgg ggttccagcc atcgcaatcg tctgcgccag gcggggcgtg
     2521 gtcgagtcga acgccggtgc ggatcttctt gtcctgaaat tcgaccaacc gcttttagag
     2581 ccggccgaga agcctgtcgg caagctcggg ggccgcgagt cgagcgcgtt tgggctttgc
     2641 tcagccatct cggccgccag caggcgcgcc tcgccagcga cgtcggcctg cgagaattcg
     2701 ccgcgtgtgg cgcccgagat gcgagcgaag tcgcgtaaat gctgctcgat gaagcgctcc
     2761 agcgcaggcc agtccagcgt gctcacgccg cgtccggcgt tgcgcggccg accaactgct
     2821 cggccagcgc cgccaccgac agcggcgccg agccttccgc cttgttgttg acggtgacgt
     2881 aggccagttc acccgcaccc gtggtgccag cgatcacgcg ggccaggtgc tggcgcgttt
     2941 ccacatcggg atcgaccaac ttgtcgaacg gctcgtactg cgccttggcc ccttcgtagc
     3001 cgtaggcgcc atggcgccgg tgcagattcc agcggcagac cagcgggccg ggccacaggg
     3061 cgcgcatcag gggcagctgg tcttcgatgg gcggcatctt ggcgtgcagg ccgaggcaat
     3121 ag
//
LOCUS       DAOMVO010000288         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9238_length_3105_cov_13.245574, whole genome shotgun sequence.
ACCESSION   DAOMVO010000288 DAOMVO010000000
VERSION     DAOMVO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9238_length_3105_cov_13.245574"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKV30_11605"
     CDS             <1..1098
                     /locus_tag="PKV30_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRL30890.1"
                     /translation="AAPQRHFLSGNPHFCRSALARFTPQDFIALLQKHGVGFHEKHKG
                     QLFCDRSAEDLIGLLLAECDAAPARDGRVERWQPCRVRGVRFQAESGSGAHQASASSY
                     FVDSDQGVMSAPALVIATGGLSIPKIGATDLGQRLARQFGLKVTELRPGLVPLTFDGA
                     AWAPYAQLAGLALPVAISTGVKKQRTTFHEDLLFTHRGLSGPAVLQISSYWQPGTPIV
                     IDLLPGVDLAAELADAKARGARKLIAGVLAAWLPQRLADAWAQQDAAWQRPIAEASDK
                     ALAQLAERLSRWQLTPTGTEGYKKAEVTVGGVDTHGLSSQTMEAKAQPGLYFIGEVVD
                     VTGWLGGYNFQWAWASAHACAEALAARKAAG"
     gene            1395..2525
                     /locus_tag="PKV30_11610"
     CDS             1395..2525
                     /locus_tag="PKV30_11610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRL30891.1"
                     /translation="MDLDRLNRYADSHGPITRGRGLLTGTIALKLAILCFLGVLAFHF
                     PQYLTTPELRRSYDVGVIRQIMFWAMVMAGSLALVNIIFNRSRWLSAFAFGLVALTVM
                     LGGHSVNVDPNFPDHTPYIGLDWFILDLLGSSLIFIFIEKLFPLRKNQPVFRPEWQAD
                     LHHFIVNHMVFGFILRATNLMVHKFFGWAANDGIRGWVAGLNFWVALFLIVLVADLVQ
                     YFTHRAYHEVPLLWRLHAVHHSAKSMDWMAGSRQHILELLITRTLLLAPIYVLGFSKE
                     VIDAHIIIVGFQAVLNHCNVNVRLGPLRFLLVTPNFHHWHHRQDQEALDKTYAAHFAF
                     LDYLFGTAVKSQRAWPSDYGVLGDYVPDGFFRQLKFPFVWKG"
     gene            complement(2488..>3105)
                     /locus_tag="PKV30_11615"
     CDS             complement(2488..>3105)
                     /locus_tag="PKV30_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL30892.1"
                     /translation="VTKATMSAHLARLVDADLVAVSAQGRHRYYRLAGHDVAELLESL
                     MGVAFRTGAVRLRSSPREPALRHARTCYDHLAGERAVAVYEALLNAGILQKDELDALA
                     LTAPGADWLKSWGLDAAALAPHSRRPLCRTCVDWSERRHHLGGALGAALLARFQALGW
                     LRRDAASRVVRFTPPGEQALTAWLRSAEDWGARLSPSTQTETSTA"
BASE COUNT          502 a         1045 c         1024 g          534 t
ORIGIN      
        1 gccgcgccgc agcggcattt cctgagcggc aacccgcact tttgccgctc ggcgctggcg
       61 cggttcacgc cgcaagactt catcgcgctg ctgcaaaagc acggcgtggg ctttcatgaa
      121 aagcacaagg gccagctgtt ttgcgaccgc tcggccgaag acctgatcgg cctgctgctg
      181 gccgaatgcg acgccgcgcc ggcgcgcgac ggacgcgtgg aacgctggca gccgtgccgt
      241 gtgcgcggcg tgcggtttca agccgaatca ggctctggcg cacatcaggc aagcgcgagc
      301 agctattttg ttgatagcga tcagggggtg atgtcggcgc cggcgctggt catcgccacg
      361 ggcggtttgt cgatcccgaa gattggcgcc accgatctcg gccagcggct ggcgcggcag
      421 tttggcctca aggtgaccga gctgcggcct ggcctggtgc cgctcacctt tgacggcgcg
      481 gcctgggcgc cctatgcgca actggccggg ctggcgctgc cggtggcgat cagcaccggg
      541 gtgaagaagc agcgaaccac ctttcatgaa gacctgctgt tcacgcaccg cggcctctca
      601 ggcccggcgg tgctgcagat ttcaagctac tggcagccgg gcacgccgat cgtgatcgat
      661 ctgctgccgg gcgtcgattt ggcggctgag ttggccgacg ccaaggcgcg cggcgcgcgc
      721 aagctgatcg cgggcgtgct ggccgcatgg ctgccgcagc gcctggccga cgcctgggcg
      781 cagcaagacg ccgcctggca gcgccccatc gccgaggcca gcgacaaggc gctggcgcaa
      841 ctggccgagc gcctgtcgcg ctggcagctc acgcccacgg gcaccgaggg ttacaagaaa
      901 gccgaggtca ccgtgggcgg cgtcgatacg catggcctgt cgtcgcaaac catggaggcc
      961 aaggcgcagc ccggcctgta cttcatcggc gaggtggtgg acgtgaccgg ctggctgggc
     1021 ggctacaact ttcaatgggc ctgggccagc gcgcatgcgt gcgccgaggc gctggcggcg
     1081 cgcaaggcgg cgggctgatg agcaaaaaca gcttgtccca tgcttgctgg cgtaaaaccc
     1141 gtcgaacccc atttccacct tcattccggg gcaagccgga gtccagacga cgtatccgac
     1201 ccaaggaacg tcagcgcagt ggcaccggct gcctggattc cggcttgcgc cggaatgacg
     1261 gcaagcagcg ccgctggcct gcgatgtgcg tcgaatggta aaaaaagccg ccagcgctcg
     1321 cacagcctgc gcaagcagct atcaataaca tagtcaccca caccaaacgc cgctggctaa
     1381 aatcgcgccc caggatcgac ctcgaccgcc tgaaccgcta cgccgacagc cacggcccca
     1441 tcacgcgcgg gcgcggcctg ctcaccggca ccatcgcgct caagctggcg atcttgtgtt
     1501 ttctgggtgt gctggcgttt cactttccgc agtacctgac cacgcccgag ctgcgccgca
     1561 gctacgacgt gggcgtgatc cggcaaatca tgttctgggc catggtgatg gcgggcagcc
     1621 tggcgctggt caacattatc ttcaaccgct cgcgctggct gtcggccttt gccttcggcc
     1681 tggtggcgct gaccgtgatg ctgggcgggc acagcgtcaa cgtggacccg aattttcccg
     1741 accacacgcc ctacatcggg ctggactggt tcattctgga cttgctgggg tcgtcgttga
     1801 tcttcatctt catcgaaaag ctgtttccgc tgcgcaagaa ccagccggtg ttcaggcccg
     1861 agtggcaggc cgatctgcac cacttcatcg tcaaccacat ggtgttcggt ttcatcctgc
     1921 gggccaccaa cctgatggtg cacaagttct tcggctgggc ggccaacgac ggcatccgcg
     1981 gctgggtggc ggggctcaac ttctgggtcg cgctgttttt gatcgtgctg gtggccgacc
     2041 tggtgcagta cttcacgcac cgcgcgtacc acgaggtgcc gctgctctgg cgcctgcacg
     2101 ccgtgcacca cagcgcgaag agcatggact ggatggccgg ctcgcgccag cacattcttg
     2161 agttgctcat cacccgcacg ctgctgctgg cgccgatcta cgtgctgggt ttttccaagg
     2221 aagtgatcga cgcccacatc atcatcgtgg gctttcaggc ggtgctcaac cactgcaacg
     2281 tcaacgtgcg gctggggcca ctgcgctttc ttctggtcac acccaacttc caccactggc
     2341 accacaggca ggaccaggaa gcgctggaca agacctacgc cgcgcacttc gcgtttctgg
     2401 actatttgtt cggcaccgcc gtcaaaagcc agcgcgcctg gccgtccgac tacggcgtgc
     2461 tgggcgacta cgtgcccgac ggcttcttca ggcagttgaa gtttccgttt gtgtggaagg
     2521 gctgaggcgc gcaccccagt cttcggcgct gcgcagccac gcggtgagcg cctgctcgcc
     2581 cggcggggtg aagcgcacca cgcgcgaggc ggcatcgcgc cgcagccagc cgagcgcctg
     2641 aaaacgcgcc agcaaggccg cgcccagcgc gccgcccaga tggtggcgcc gctcgctcca
     2701 gtcgacgcag gtcctgcaca acgggcggcg gctgtgcggc gcgagggctg cggcgtccag
     2761 cccccagctc ttgagccaat cggcccctgg cgcagtcagg gcaagcgcgt ccagctcatc
     2821 tttttgtagt atgccggcgt tcagcaaggc ttcgtacacg gccaccgcgc gctcgccggc
     2881 caggtggtcg tagcaggtgc gcgcgtggcg cagcgccggc tcgcgcgggc tgctgcgcaa
     2941 gcgcacggcg ccggtgcgaa aggccacgcc catcaggctt tccagaagct cggccacatc
     3001 gtgccccgcc agccggtagt agcggtgccg gccctgcgcc gacacggcga ccaggtcggc
     3061 gtccaccagc cgcgcgaggt gcgcgctcat ggtcgccttg gtcac
//
LOCUS       DAOMVO010000289         3096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9280_length_3096_cov_17.876685, whole genome shotgun sequence.
ACCESSION   DAOMVO010000289 DAOMVO010000000
VERSION     DAOMVO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9280_length_3096_cov_17.876685"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKV30_11620"
     CDS             complement(<1..579)
                     /locus_tag="PKV30_11620"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HRL30893.1"
                     /translation="MTILDFDAPAVRQLRVDLALALRAAAHHGLAEGVCNHFSVELPD
                     GSGRFLLNPRGLMWAEVQADDVVMVDAQGTRLAGRHEVEPTAMFIHAAIHRLCRKACV
                     LHTHMPYATALTLTTDRALDTTLSQNAMRFHGRTAIDARYNGLALDAREGERIAAAMG
                     DADVGFLSNHGVVVCGARIDHAYDDLYYLERAC"
     gene            698..1180
                     /gene="hpaR"
                     /locus_tag="PKV30_11625"
     CDS             698..1180
                     /gene="hpaR"
                     /locus_tag="PKV30_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HRL30894.1"
                     /translation="MSRAAPFAHRNLPLLLLRAREAVMQHRRPSLRAQGLSDQQWRVL
                     RVLAEHTEGLETGQVAREAHLLGPSLTGVLVRMARDGWVTRERSAHDARRSVVRITAE
                     GQTLVCALSADIEAQYADLERHLGRARLTQLYGLLDELIALQEAGDECAAHELLETAA
                     "
     gene            1177..1878
                     /locus_tag="PKV30_11630"
     CDS             1177..1878
                     /locus_tag="PKV30_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRL30895.1"
                     /translation="MSAALRWLPQGTVYGALLNFRREWDLWAPQMAQAPYKAAPQAPV
                     LYIKTANTFAPCGAAIAVPDEVDVGATLGLVIGGFEEKLPPALAESAQAATKKEAGGL
                     HRIAGCVLLNDLSLPHASYYRPPIKFRNRDGFLGCGPVLQAVPDLSTLALELRINGTW
                     AQRIELPTLVRDAATLLRDVSEFTTLQAGDVLMLGTDCLPDGTRPRVKAGDRIEISAP
                     GFAPLVHTLIEEKAT"
     gene            1875..2684
                     /locus_tag="PKV30_11635"
     CDS             1875..2684
                     /locus_tag="PKV30_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRL30896.1"
                     /translation="MKRARIAWAGAVHDAIERDGRLELLTPAFKNRLLDFGDVVWLPP
                     LAPMPPHRPRTVLALAINYADHAKELAFKPPEEPLAFAKGAASLIGHRAFTVRPDGVQ
                     NMHYECELAVVIGREARHVKRGNAYDFVRGYTVANDYAIRDYLENWYRPNLRVKNRDT
                     CTPIGPWLVDAADVPDPMKLQLRTTVNCTVTQQGNTRDMVFDVPTLIEYFSSFMTLYP
                     GDLILTGTPDGVVDCPVGSHVVCEIEGIGALENTIIEETGRQRPSDKREQL"
     gene            2681..>3096
                     /locus_tag="PKV30_11640"
     CDS             2681..>3096
                     /locus_tag="PKV30_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL30897.1"
                     /translation="MKIDHLINGKAVPGDTYFETVNPATQQVLAEVAAGGEAEVNAAV
                     SAAKDAFPKWAGLPATERAKKIRALGELIAQHVPEISTTETDDTGQTISQTAKQLVPR
                     AADNFSYFAEMCTRVDGHTYPTPTHLNYTLFHSVGV"
BASE COUNT          546 a         1048 c         1046 g          456 t
ORIGIN      
        1 gcaggcgcgc tccaggtagt acaggtcgtc gtaggcgtgg tcgatgcgcg cgccgcacac
       61 caccacgccg tggttgctca agaaacccac gtcggcatcg cccatggcgg cggcgatgcg
      121 ctcgccctcg cgcgcgtcca gcgccaggcc gttgtagcgc gcgtcgatgg ccgtgcggcc
      181 atgaaagcgc atggcgtttt gcgagagcgt ggtgtcgagc gcgcgatcgg tcgtcaacgt
      241 cagcgccgtg gcgtacggca tgtgcgtgtg cagcacacag gccttgcggc acaggcggtg
      301 gatggcggcg tggatgaaca tggccgtcgg ctccacctcg tgccgccccg ccagccgcgt
      361 gccttgcgcg tccaccatca ccacgtcgtc ggcctgcact tcggcccaca tcagcccgcg
      421 cgggttgagc agaaagcgcc ctgagccatc gggcagctcg acgctgaaat ggttgcacac
      481 gccctcggcc aggccgtggt gcgcggcggc gcgcagggcc agcgccaggt cgacgcgcaa
      541 ctggcgcacg gcgggggcgt cgaagtcgag gatagtcatg gagactccag ggtggcggtc
      601 acatcacgca ttgcagcgta cacgcgaccg acaggtgctg ccggcgggca cgctcaaaac
      661 gcttgcgtcg cgagcattta tttaatacat taaataaatg tcccgcgccg ccccgtttgc
      721 ccaccgaaac ctgcccctgc tgctgctgcg cgcgcgcgaa gcggtgatgc agcaccggcg
      781 gcccagcttg cgggcgcagg gcttgtcgga ccagcagtgg cgcgtgctgc gcgtgctggc
      841 agagcacacc gaggggctgg agacgggcca ggtcgcgcgc gaggcgcacc tgctggggcc
      901 gagcctgacg ggcgtgctgg tgcgcatggc gcgcgatggt tgggtcacgc gcgagcgcag
      961 tgcgcacgac gcgcggcgca gcgtggtgcg catcaccgcc gaaggccaga cgctggtgtg
     1021 cgcgctgtcg gccgacatcg aggcgcagta cgccgacctg gagcgccacc tgggccgcgc
     1081 gcgcctgacg cagctgtacg gcctgctgga cgagttgatc gcgctgcagg aagcgggcga
     1141 cgagtgcgcc gcgcacgagt tgctggagac cgccgcgtga gcgccgcttt gcgctggctg
     1201 ccgcaaggca cggtgtacgg cgcgctgctc aacttccggc gcgagtggga tctgtgggcg
     1261 ccgcagatgg cgcaggcgcc ctacaaggcc gcaccccagg cgccggtgct gtacatcaag
     1321 acggccaaca cgttcgcccc ttgcggcgcg gccatcgccg tgccggacga ggtggacgtc
     1381 ggcgccacgc tgggcctggt gatcggtggc tttgaagaaa aactgccgcc agcgcttgct
     1441 gaatcagcgc aagcagctac aaaaaaagaa gctggcggcc tgcacagaat cgccggctgc
     1501 gtgctgctga acgacctgag cctgccgcac gccagctact accgcccgcc catcaagttc
     1561 cgcaaccgcg acggctttct cggttgcggc ccggtgctgc aggccgtccc tgatctgagc
     1621 acgctggcgc ttgagctgcg catcaacggc acgtgggcgc aacggatcga gctgcccacg
     1681 ctggtgcgcg acgcggccac cctgttgcgc gatgtaagcg aattcacgac gctgcaagcc
     1741 ggcgacgtgc tgatgctggg caccgactgc ctgccggatg gcacgcgccc gcgcgtcaag
     1801 gcgggggatc gcatcgagat cagcgcgccc ggcttcgcgc cgctggtgca cacgctgatc
     1861 gaggagaagg ccacatgaag cgcgcccgca tcgcctgggc cggcgccgtg cacgacgcca
     1921 tcgaacgcga cggccggctg gagttgctga cgcctgcgtt caaaaaccgc ctgctggact
     1981 ttggcgacgt cgtctggctg cccccgctgg cgcccatgcc gccgcaccgc ccgcgcaccg
     2041 tactggcgct ggccatcaac tacgccgacc acgcgaaaga gctggccttc aagccgcccg
     2101 aagagccgct ggcgtttgcc aagggcgccg cctcgctgat cggccaccgc gccttcacgg
     2161 tgcggcccga tggcgtgcag aacatgcact acgaatgcga gctggcggtg gtcatcggcc
     2221 gcgaagccag gcatgtgaag cgcggcaacg cctacgactt cgtgcgcggc tacaccgtgg
     2281 ccaacgacta cgcgatccgc gactacctgg aaaactggta ccgcccgaat ctgcgcgtga
     2341 agaaccgcga cacctgcacg cccattggcc cgtggctggt cgacgcggcc gatgtgccgg
     2401 atccgatgaa gctgcaactg cgcaccacgg tcaactgcac cgtcacgcaa cagggcaaca
     2461 cgcgcgacat ggtgttcgat gtgccgactt tgatcgaata cttcagcagc ttcatgacgc
     2521 tgtaccccgg cgatctgatc ctgaccggca cgcccgacgg cgtggtcgat tgcccggtgg
     2581 gcagccacgt ggtgtgcgag atcgaaggca tcggcgcgtt ggagaacacc atcattgaag
     2641 aaacagggcg ccagcgcccg tcagacaagc gcgagcagct atgaaaattg accatctcat
     2701 caatggcaaa gcggtgcccg gagacaccta tttcgaaacc gtgaaccccg ccacgcagca
     2761 ggtgctggcg gaagtggcgg cgggcggcga ggctgaagta aatgccgccg tttccgccgc
     2821 caaagacgcc tttccgaaat gggccggcct gccggccacc gagcgcgcca agaagattcg
     2881 cgcgctgggc gagttgatcg cccagcatgt gcccgaaatc tcgaccacag agaccgacga
     2941 caccggccag accatcagcc aaacggctaa gcagctggtg ccgcgcgcgg ccgacaactt
     3001 cagctacttc gccgagatgt gcacgcgcgt cgacggtcac acatacccca cgccgacgca
     3061 cctgaactac acgctgttcc actcggtcgg cgtgtg
//
LOCUS       DAOMVO010000290         3071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9396_length_3071_cov_11.491379, whole genome shotgun sequence.
ACCESSION   DAOMVO010000290 DAOMVO010000000
VERSION     DAOMVO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9396_length_3071_cov_11.491379"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..826)
                     /locus_tag="PKV30_11645"
     CDS             complement(56..826)
                     /locus_tag="PKV30_11645"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HRL30898.1"
                     /translation="MATSSTRFAGIDALRGAAMLWMTAYHFCFDLQHFGYTRQNFYED
                     PFWTWQRTGIVSLFLLCAGAGQAIAVLQGQSWPRFWRRWAQVVGCAALVSLGSWWMFP
                     HSWISFGVLHGMALMLLMVRWLLVRGWLRGATPWVLGLGAVLAAPLLSALLQSHGSPA
                     LGAALQSRWWNWLGVVTEKPPTEDWVPLLPWLGVMLWGAGAAQWWWQRRRSRAVRTPG
                     AAERALGLLGRWSLSYYMLHQPVLIGLLMAFRALSAAY"
     gene            1034..>3071
                     /locus_tag="PKV30_11650"
     CDS             1034..>3071
                     /locus_tag="PKV30_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRL30899.1"
                     /translation="MTASDSTPPHRRPLLHWVLGLVAALLVATAAALAWLTWNDWNRS
                     RGWVSEKVSQAIGRDFAIRGPLDLDWQWPQQMESGWRRWIPTPVVTAHDVLIGNPPDF
                     AAKGPHFAHLARATADIALLPLLARRIEIRSVALTEPDVRLERLAEKNNNWTFDFRRE
                     SGAEPRWSVSLGRLLLSKGELGYDDALRKLSVSGTVDTLPADQTEDGRYGIGFDFSGW
                     QGKAEVRGSGKAGQLLSLREEQLDYPLKLDARAGRLGATAEGTIANPRQLSGVDLQVN
                     LKGGSLADLFPLTGIVLPDTPPFQTRGQLVGTLKPDGAVWQYQGFTGTVGKSDLAGDV
                     TYTSAQPRPILKGTMKSKLLRLEDLGPVVGAKSNNPDKKQRAGKVLPDDPFDTSRWDK
                     MDLDLQYTGQRIERPQAVPLDSLRAHAVMDNAQLKLAPLDFGIAKGRFTTQVLLNSRT
                     KPMQVSLRGDVKNLQLSALFPKIELMKKSLGDVDGGIALDGRGQSVAQILATANGQAK
                     LYVRDGVMSQQLLDLAGLNLGSVIVSKLFGQDKEVRLRCAIADVPVRNGVAYMQNVKV
                     NTDDALIEIDGTADMRQEMFDIDVNPKAYSLKFFSLRTPLEVRGPFIKPHVGVKPGPL
                     IVRAAAAVAALAAAPGALVLVPITVPGAADNESCGPLLAKATRDPKAGAPAAVAS"
BASE COUNT          546 a         1099 c         1007 g          419 t
ORIGIN      
        1 tctcaatagc tgctcgcgct tgccagacaa gcgccagcgc cgcttttctt ccagatcaat
       61 acgctgcgga cagggcgcga aaggccatca acagcccgat cagcaccggc tggtgaagca
      121 tgtagtagct gaggctccag cgccccagca gccccaacgc gcgctcggcc gcgcccggcg
      181 tgcgcaccgc gcgcgacctg cgacgctgcc accaccattg cgccgcgccc gcgccccaca
      241 gcatcacgcc cagccacggc agcagcggca cccaatcctc ggtcggcggt ttttcggtga
      301 ccacgccgag ccagttccac caccggcttt gcaaggcggc gcccagcgcg ggtgagccgt
      361 gcgattgcag cagcgcgctc aaaagcggcg ctgccagcac cgcccccaag cccagcaccc
      421 agggcgtggc gccgcgcagc cagccgcgca ccagcagcca gcgcaccatc agcagcatca
      481 gcgccatgcc gtgcagcacg ccaaagctga tccagctgtg cggaaacatc caccacgaac
      541 ccagcgatac cagcgccgcg caccccacca cctgcgccca gcgacgccag aagcgcggcc
      601 agctctggcc ttgcagcacg gcgatggcct gcccggcgcc ggcgcacagc aaaaacaggc
      661 tgacgatgcc ggtgcgctgc caggtccaga acgggtcttc atagaagttc tgccgcgtgt
      721 agccaaagtg ctgcaggtcg aagcagaagt ggtaggcggt catccacagc atggccgcgc
      781 cgcgcagggc gtcgatgccg gcaaaacgcg tggatgaagt ggccatgtcg gacaagtgcg
      841 cacaggggcc gccgacgggc ggcgcatgac gctgtaggaa gaagacgaaa gcggcgatct
      901 tagccatcgc cagggcgtcc gcgccgctag gatgggccga tccgttgaac cctggcgcgc
      961 caggtcgccg cctttgaccg agcccgacct ccgagcgtgc ccgagcgccc ccgatggggc
     1021 gcagtgaccc gagatgaccg cttccgattc cacgccgccg caccgccgcc ctcttttgca
     1081 ctgggtgctg ggccttgtcg ccgcgctttt ggtggccacg gccgccgcgc tggcctggtt
     1141 gacctggaac gactggaacc gctcgcgcgg ctgggtgtcc gaaaaagtct cgcaggccat
     1201 cggccgcgat ttcgctattc gcggcccgct cgacctggac tggcagtggc cgcagcagat
     1261 ggaaagcggc tggcggcgct ggattcccac cccggtggtg acggcgcacg acgtgttgat
     1321 cggcaatccg cccgactttg ccgccaaggg cccgcacttt gcgcacctgg cccgcgccac
     1381 cgccgacatt gcgctgctgc cgctgctggc gcggcgcatc gagatccgca gcgtggcgct
     1441 gaccgagccc gacgtgcggc tggagcgcct ggcagaaaag aacaacaact ggaccttcga
     1501 cttccgccgc gaatccggcg ccgagccgcg ctggagcgtc agcctgggcc gcctgctgct
     1561 ctcaaagggc gagctgggct acgacgacgc gctgcgcaag ctcagcgtgt cgggcaccgt
     1621 cgacaccctg cccgccgacc agaccgaaga cggccgctac ggcatcggct tcgacttttc
     1681 cggctggcag ggcaaggccg aagtgcgcgg cagcggcaag gccgggcaac tgctgtcgct
     1741 gcgcgaggaa caactcgact acccgctcaa gctcgacgcg cgcgctggcc gcctgggcgc
     1801 cacggccgag ggcaccatcg ccaacccgcg ccaactgtcg ggcgtggatt tgcaggtcaa
     1861 cctcaagggc ggcagcctgg ccgatctgtt tccgctcacc ggcatcgtgt tgcccgatac
     1921 gccgcccttc cagacccgcg gccaactggt cggcacgctc aagcccgatg gcgccgtgtg
     1981 gcaataccag ggcttcaccg gcacggtcgg caaaagcgat ctggcgggcg acgtgaccta
     2041 cacctcggcc cagccgcgcc cgatattgaa gggcacgatg aagtccaagc tgctgcggct
     2101 cgaagacctg gggccggtgg tcggcgccaa gtcgaacaac cccgacaaga agcagcgcgc
     2161 cggcaaggtg ctgcccgacg accccttcga caccagccgc tgggacaaga tggacctgga
     2221 cctgcagtac accggccagc gcatcgaacg cccgcaggcc gtgccgctcg acagcctgcg
     2281 cgcgcatgcc gtgatggaca acgcccagct caagctggcg ccgctcgact ttggcatcgc
     2341 caagggccgt ttcaccacgc aggtgctgct gaattcgcgc accaagccga tgcaggtgtc
     2401 gctgcgcggc gacgtgaaga atctgcagtt gtcggcgctg tttcccaaga tcgagctgat
     2461 gaagaaaagc ctgggcgatg tcgatggcgg catcgcgctc gacgggcgcg gccagtccgt
     2521 cgcgcaaatc ctggccaccg ccaatggcca ggccaagctc tacgtgcgcg acggcgtgat
     2581 gagccagcaa ctgcttgacc tggccgggct caacctcggc agcgtcatcg tcagcaagct
     2641 gttcggacag gacaaggaag tgcgcctgcg ctgcgccatt gccgacgtgc cggtgcgcaa
     2701 tggcgtggct tacatgcaaa acgtgaaggt caacaccgac gacgcgctga tcgagatcga
     2761 cggcacggcc gacatgcggc aggagatgtt cgacatcgac gtcaacccca aggcctacag
     2821 cctcaagttc ttctcgctgc gcacgccgct ggaagtgcgc gggccgttca tcaagccgca
     2881 cgtgggcgtc aagcccggcc cgctgatcgt gcgcgcggcc gccgccgtgg ccgcgctggc
     2941 ggccgcaccg ggcgcgctgg tgctggtgcc catcacggtg ccgggcgcgg cggacaacga
     3001 aagctgcggc ccgctgctgg ccaaggccac gcgcgacccc aaggcgggcg cgccagcggc
     3061 ggtggcgtcg g
//
LOCUS       DAOMVO010000291         3054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9463_length_3054_cov_11.477826, whole genome shotgun sequence.
ACCESSION   DAOMVO010000291 DAOMVO010000000
VERSION     DAOMVO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9463_length_3054_cov_11.477826"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1025)
                     /locus_tag="PKV30_11655"
     CDS             complement(87..1025)
                     /locus_tag="PKV30_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL30900.1"
                     /translation="MPDAESADLIDQLSHRHIEVFRAVMQAGQVTRAAEALRSSQPTV
                     SRELARLEHILGYALFDRIQGRLRPTVRARALYDEVERAWQGLARVQASARELRQFAT
                     GRLHLACLPALAHALVPPALRRFAAQHPQASVTVVPMESPALEAALSEQQADLGLTEA
                     RSAPVGCELHPMLEADEVVVLPAAHPLAAREVLALADFAGQPFINLALADPYRAQIDA
                     LFAAAGVARAARLESASAVSICALVAEGLGLSIINPLTARAMAAGDARLVVRPLAASV
                     RFQVALVRPLWRAEHPLLGDLQGALVAAASGGEEVV"
     gene            1095..2360
                     /gene="lysA"
                     /locus_tag="PKV30_11660"
     CDS             1095..2360
                     /gene="lysA"
                     /locus_tag="PKV30_11660"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRL30901.1"
                     /translation="MDPFSDDLLTTLARQHGTPLWVYDAATIRQRIAELRAFDTVRYA
                     QKANSNLSLLKLMREQGVAVDAVSRGEIERALAAGYTADPMPGSTASAMVFTADLLDA
                     ETLATVVRHRVPVNAGSIDMLHQLGAASPGHAVWLRINPGFGHGHSNKTNTGGEHSKH
                     GIWHAALGEALDAVQQHGLKLVGLHMHIGSGVDYGHLQQVCRTMVQLVAQAGAARLPD
                     LHAISAGGGLSIPYQADQPRIDTAHYFSLWDAARREVAALLGRPVALEIEPGRYLVAE
                     SGVLLTEVRAVKSQGSQRFTLVDAGFNDLMRPAMYGAHHGMSLIAGSNTPPISQRPLR
                     ETVVAGPLCESGDVFTQAEGGVVLTRALPEAQVGDLLVIHDAGAYGASMSSNYNTRGL
                     AAEVLVDGGAQRLIRRRQTVAELLALEAV"
     gene            2391..2837
                     /locus_tag="PKV30_11665"
     CDS             2391..2837
                     /locus_tag="PKV30_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRL30902.1"
                     /translation="MSLKDQITEDMKTAMRAKDSGRLGTIRLLQAAMKQKEVDERVTL
                     DDAMVIAIVDKLIKQRKDSIDAFTKADRQDLVDIEEAEMKVLQVYLPARLSADEVAAE
                     VKAIVAELGASGPGDMGKVMGAVKQRLAGKADMGAVSAAVKAALAG"
BASE COUNT          550 a         1048 c         1037 g          419 t
ORIGIN      
        1 tttttcatgc atgaccgaaa ggcagtcgct acaaaaaaaa gagctgcttg cgcttactcc
       61 acaagcgcca gaggccaaaa tgaccctcaa accacctctt ccccaccgct cgccgccgcc
      121 accagcgcgc cctgcaaatc ccccagcagc ggatgctcgg cccgccacag cggtcgcacc
      181 agcgccacct gaaagcgcac cgacgccgcc agcggccgca ccaccagccg cgcatcgccg
      241 gcggccatgg cccgggcagt gagcgggttg atgatggaca ggcccagccc ctcggccacc
      301 agcgcgcaga tggagacggc gctggcgctt tccagccgcg ccgcgcgcgc cacgccggcg
      361 gcggcaaaca gggcgtcaat ctgcgcgcgg tacgggtcgg ccagcgccag gttgatgaag
      421 ggctggccgg cgaaatcggc cagcgccagc acctcgcgcg cggccagcgg gtgcgcggcg
      481 ggcagcacaa ccacttcgtc ggcctccagc atcgggtgca gctcgcagcc cacgggggcg
      541 ctgcgcgctt cggtcagacc caagtcggcc tgctgttcgg acaaggccgc ctccagcgcg
      601 ggcgactcca tgggcacgac cgtaacgctg gcctgcgggt gctgggcggc aaagcgccgc
      661 agcgcgggcg gcaccagcgc gtgcgccaat gcgggcaggc aggccaggtg caggcggccg
      721 gtggcgaact gccgcagctc gcgcgcgctg gcctgcacgc gcgccaggcc ctgccaggcg
      781 cgctcgacct cgtcgtacag cgcacgcgca cgcaccgtgg ggcgcaggcg tccctgaatg
      841 cgatcaaaca gcgcgtagcc caggatgtgt tcgaggcgcg ccagttcgcg gctgacggtg
      901 ggctgtgagc tgcgcagcgc ctcagccgca cgcgtgacct gcccggcctg catgacagcg
      961 cggaatacct cgatgtggcg atgtgagagc tggtcgatca gatcggcaga ttcagcgtcg
     1021 ggcatatcaa aaatgaattc agactgaaaa aacagctatt ttgacgaatg tctgcttggc
     1081 ccgatcatcg gcgcatggat cctttttctg acgatctgct caccaccctc gcccgccagc
     1141 acggcacgcc gctgtgggtg tacgacgccg ccaccatccg ccagcgcatt gccgagctgc
     1201 gcgcgttcga cacggtgcgc tatgcgcaaa aggccaactc caacctgagc ctgctcaagc
     1261 tgatgcgcga acaaggcgtg gcggtcgatg ccgtgtcgcg cggcgagatc gagcgcgcgc
     1321 tggcggcggg ctacacggcc gatcccatgc ccggctccac ggccagcgcc atggttttca
     1381 ccgccgatct gctggacgcc gagaccctcg ccaccgtggt gcgccaccgc gtgccggtca
     1441 acgccggctc catcgacatg ctgcaccagc tgggcgcggc atcgcccggc cacgccgtgt
     1501 ggctgcgcat caaccccggc tttggccacg gccacagcaa caagaccaac accggcggcg
     1561 aacacagcaa gcacggcatc tggcacgccg cgctgggcga ggcgttggat gcggtgcagc
     1621 agcacggcct caagctggtg gggctgcaca tgcacatcgg ctcgggcgtg gactacgggc
     1681 acttgcagca ggtctgccgg acgatggtgc agctggtggc gcaggcgggc gcggcgcggc
     1741 tgcccgactt gcacgcgatt tcagcgggcg gcggcttgtc gattccctac caggccgatc
     1801 agccccgcat cgacaccgcg cactacttca gcctgtggga cgccgcccgg cgcgaagtgg
     1861 ccgcgctgct gggccgcccg gtggcgctgg agatcgagcc gggccgctac ctggtggccg
     1921 aaagcggcgt gctgctgacc gaggtgcgcg ccgtcaagtc gcagggcagc cagcgcttca
     1981 cgttggtgga tgcgggcttc aacgacctga tgcgcccggc catgtacggc gcgcaccacg
     2041 gcatgagcct gatcgcgggc agcaacacgc cgccgatcag ccaacgcccg ctgcgcgaga
     2101 ccgtggtcgc cggcccgctg tgtgaatcgg gcgacgtgtt cacgcaggcc gagggcggcg
     2161 tggtgctgac gcgcgcgctg cccgaggcgc aagtgggcga cctgctggtg atccacgacg
     2221 cgggtgccta cggcgccagc atgtcgagca actacaacac gcgcggcctg gccgccgagg
     2281 tgctggtcga tggcggcgcg cagcgcctga tccgccgacg ccagaccgtg gccgagctgc
     2341 tggcgctgga ggcggtgtaa gacaatcgcc gttttgacga gacacacgcc atgagcctga
     2401 aagaccagat caccgaagac atgaaaaccg ccatgcgcgc caaagacagc gggcgcctgg
     2461 gcaccatccg cctgctgcag gcggcgatga agcaaaaaga ggtggacgag cgcgtgacgc
     2521 tggacgacgc gatggtgatc gccatcgtcg acaagctcat caagcagcgc aaggacagca
     2581 tcgacgcctt caccaaggcc gaccggcagg atctggtgga catcgaagaa gccgagatga
     2641 aggtgttgca ggtctacctg cccgcgcgcc tctcggccga tgaagtggcc gccgaagtga
     2701 aagccatcgt ggccgagctg ggtgccagcg ggccgggcga catgggcaag gtgatgggcg
     2761 ccgtcaagca gcggctggcc ggcaaggcgg acatgggtgc ggtcagcgcg gcggtcaagg
     2821 cggcgctggc gggctgatca aaagccacgc cataaaatat caagttgata gctgctcgcg
     2881 cttgctggac aagcgccagc ggcttatttt attgatattc cagccgcacc acgcccgcgc
     2941 acacccgcaa cattcgcttg caccccgaac ctccagcttt gcacgccggg cgccagcgcg
     3001 gcaaaccgaa tcgcaccgcc gcgcgttagg tgaggtgtgg gaacacgccg ttcc
//
LOCUS       DAOMVO010000292         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9470_length_3052_cov_12.571905, whole genome shotgun sequence.
ACCESSION   DAOMVO010000292 DAOMVO010000000
VERSION     DAOMVO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_3052_cov_12.571905"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..707)
                     /locus_tag="PKV30_11670"
     CDS             complement(21..707)
                     /locus_tag="PKV30_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRL30903.1"
                     /translation="MIVTHRLIVRLRRHPSSALLAAQLLGVLLYPLLQDSASGQIALA
                     VFGLVVLGLALRMVRRSPAATQSALALAACVVVFSVWNVLAPDRRLLLAVSLLEAAFY
                     FYAAGALIRYMLQDDVATTDELVAAGATFTVLAWAWAHLYMACQVLAPGSFIAAVRPD
                     AARSWFELLFLSFTSLSSVGLGDVVPVKPMARALVMLEEFAGVMYIALVVSRLVGLLT
                     LRRIEQHPPQ"
     gene            762..1574
                     /locus_tag="PKV30_11675"
     CDS             762..1574
                     /locus_tag="PKV30_11675"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRL30904.1"
                     /translation="MSSRPIRHQYEDFMCHVFEHGVFKADRTGTGTKSVFGYQMRFDL
                     SEGFPLVTTKKVHLKSIIYELLWFLRGDGNARWLQERGVTIWDEWAKPDGDLGPVYGV
                     QWRSWPKPDGGHIDQIAQVVQTLKTNPDSRRIIVSAWNVADLDRMALMPCHAFFQFYV
                     ADGKLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLAVGDFIWTGGDCHIYSNH
                     EAQVKEQLSRAPFPYPTLHIRRRPPSIFDYEFDDFEVQGYQHHPAIKAPVAV"
     gene            1643..2644
                     /locus_tag="PKV30_11680"
     CDS             1643..2644
                     /locus_tag="PKV30_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRL30905.1"
                     /translation="MTDLLSLYRTMVRIRAFEDAAEVASQGGVAAWGKEASATPALVR
                     GPLHLSTGQEAVAAGVCAHLTPADLLTSTHRGHGHTLAKGADPTKMMCELFGRATGYN
                     GGKGGSMHIADFSVGMLGANGVVAAGLPIAVGAAQGLKIRGQDAIAVCFFGDGAINRG
                     PFMEALNWAVVYHLPVLFVCEDNRISATTASAPMTAGPGASARASAMGIAAVQVDGTD
                     VQAVSNAAEELIREIRAGSGPRLLHALTDRHKGHVSVDPGTYRDPDAVAAALARDGIG
                     RTRAQLVAQGHAAQVEQIEREAKTEIDAAVATATAAPLPDKSAAYTDIQSTGAGVWQ"
     gene            2659..>3052
                     /locus_tag="PKV30_11685"
     CDS             2659..>3052
                     /locus_tag="PKV30_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRL30906.1"
                     /translation="MTYAQAAALALSQAMARDPNVIALGEDLGRGGVFGQYRDADGEP
                     LVRRFGPDRIIDTPISESTIVGAAVGMALAGCTPVVELRVVDFALCAIDEVVNQAAKN
                     RYMFGGQGRVPMVMRMPIGIWSSSAAQHS"
BASE COUNT          540 a         1028 c         1024 g          460 t
ORIGIN      
        1 aagcaaagcg ccgcgcaggg tcattgcggc gggtgctgtt cgatgcggcg cagcgtgagc
       61 agccccacca ggcgcgatac caccagcgcg atgtacatca cgccggcaaa ctcttccagc
      121 atgaccagcg cgcgcgccat tggcttgacg ggcaccacgt cgcccaggcc cacgctggac
      181 agcgacgtga aactgaggaa cagcagctcg aaccaactgc gcgccgcatc gggccgcacg
      241 gcggcgatga agctgcccgg cgccagcacc tggcacgcca tgtacaggtg cgcccaggcc
      301 caggccagca cggtgaaggt ggcgccggcg gccaccagtt cgtcggtggt ggccacgtcg
      361 tcctgcagca tgtagcggat cagcgcgccg gcggcgtaga agtagaacgc cgcctccagc
      421 agcgacaccg ccagcagcag acgccggtcg ggcgccagca cgttccacac cgaaaacacc
      481 accacgcacg ccgccagcgc cagcgccgac tgcgtggccg ccgggctgcg ccgcaccatg
      541 cgcagcgcca gccccagcac caccaggcca aacaccgcca gcgcaatctg cccgctggcc
      601 gaatcctgca gcagcggata cagcaacacg cccagcagtt gcgcggccag cagcgcggac
      661 gacgggtggc ggcgcaggcg cacgatgagg cgatgggtga cgatcatgac cgccgattgt
      721 cgccaaacgt ggggcgcttg cgcatgcgcg agaatcggcg catgagcagc cgcccgatcc
      781 gccaccagta cgaagatttc atgtgccacg tgttcgagca cggcgtgttc aaggccgacc
      841 gcactggtac cggcaccaag agcgtgttcg gctaccagat gcggtttgat ttaagcgagg
      901 gctttccgct cgtcaccacc aagaaggtgc acctcaaaag catcatctac gagctgctgt
      961 ggtttttgcg cggcgacggc aacgcgcgct ggctgcaaga gcgcggcgtg acgatctggg
     1021 acgagtgggc caagcccgat ggcgacctgg ggccggtgta cggtgtgcag tggcgcagtt
     1081 ggcccaagcc ggatggcggc cacatcgacc agatcgcgca ggtggtgcag acgctgaaga
     1141 cgaaccccga ctcacgccgc atcatcgtga gcgcctggaa cgtggccgat ctggaccgga
     1201 tggcgctgat gccgtgccac gcgttctttc agttctacgt ggcggatggc aaattgagtt
     1261 gccagcttta tcagcgctca gccgacatct ttctgggcgt tcccttcaac atcgccagct
     1321 acgcgctgtt gacgcacatg ctggcgcagc agtgcgatct ggctgtgggc gacttcatct
     1381 ggacgggtgg cgattgccac atctacagca accacgaagc gcaggtgaag gagcaactga
     1441 gccgcgcgcc cttcccctac cccacgctgc acatcaggcg ccgcccgccc tccatcttcg
     1501 actacgaatt cgacgatttc gaggtgcagg gttatcagca ccacccggcc atcaaggcgc
     1561 cggtggcggt gtagttttga atcgaattgg cccctggcgc ttgcccatca agcgcgagta
     1621 gctatccatt ttgaagcaac gaatgaccga tctgttatcg ctctaccgca ccatggtgcg
     1681 catccgcgcc ttcgaggacg cggccgaagt cgccagccag ggcggcgtgg cggcctgggg
     1741 caaggaggcg tcggccacgc cggcgctggt gcgcgggccg ctgcatttgt ccacgggtca
     1801 ggaggccgtg gcggcgggcg tgtgcgcgca cctgacgccc gccgacctgc tcaccagcac
     1861 gcaccgcggc cacggccaca cgctggccaa gggcgctgat ccgacgaaga tgatgtgcga
     1921 gctgttcggc cgcgccacgg gctacaacgg cggcaagggc ggctcgatgc acatcgcgga
     1981 cttctcggtg ggcatgctgg gtgccaacgg cgtggtggcc gctggcctgc cgattgccgt
     2041 gggcgctgcg caggggctga agattcgcgg gcaggatgcc atcgccgtgt gtttcttcgg
     2101 cgacggcgcc atcaaccgcg ggccgttcat ggaggcgctc aactgggccg tggtgtatca
     2161 cctgccggtg ctcttcgtgt gcgaggacaa ccgcatcagc gccaccaccg cgagcgcgcc
     2221 aatgacggct ggacccggcg ccagcgctcg cgcatccgct atggggattg cagcggttca
     2281 agtcgatggc acggatgtgc aagcggtgtc gaacgccgcc gaggaactga tccgcgagat
     2341 ccgcgccggc agcggcccac gcctgctgca cgcgctgacg gatcggcaca aggggcatgt
     2401 gtcggtcgat cctggcacct atcgcgaccc ggatgccgtg gcggcggcgt tggcccgcga
     2461 cggcattggc cgcacgcgcg cgcaactcgt cgcacaaggc catgccgcgc aggtcgagca
     2521 gatcgagcgc gaggccaaga ccgagatcga cgccgctgtc gccaccgcca ccgccgcgcc
     2581 gctgccggat aaatccgccg cctacaccga catccagagc accggcgcgg gagtgtggca
     2641 atgagcgccc cggttcaaat gacttacgcg caggccgccg cgctggcctt gtcgcaggcg
     2701 atggcgcgtg atcccaacgt cattgcgctg ggcgaagacc tgggacgcgg cggcgtgttc
     2761 ggccaatacc gcgatgccga cggtgagccg ctggtccgac gctttggccc ggatcgcatc
     2821 atcgacacgc cgatctccga aagcaccatc gtcggcgccg ccgtcggcat ggcgctggcc
     2881 ggctgcacgc cggtggtcga gttgcgcgtg gtcgatttcg ccctgtgcgc cattgacgaa
     2941 gtggtgaacc aggccgccaa gaaccgctac atgttcggcg gccagggccg cgtgccgatg
     3001 gtgatgcgca tgcccatcgg catctggtcg tcgtcggccg cgcagcacag cc
//
LOCUS       DAOMVO010000293         3017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9595_length_3017_cov_10.969953, whole genome shotgun sequence.
ACCESSION   DAOMVO010000293 DAOMVO010000000
VERSION     DAOMVO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_3017_cov_10.969953"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PKV30_11690"
     CDS             <1..1120
                     /locus_tag="PKV30_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRL30907.1"
                     /translation="RGGRGGSRNERGDRASDERRQEGRAEGQGEARSGQRDGSRRNGE
                     RNERGDRGPRTDRQGPRERRDALPGDGANAAQTADAAALDTASDNAARAPRGERSERG
                     EGRNRRGGEGRRDRGERGDRAERSDRPDAPPQDDALRADSAMAAAAVPEGAAPDHAGE
                     AGNGEERRERRSRDRYGRDRRERDGERRDEPRADLPGAVADDGSVVAAPASADPSSPR
                     SYFARSGDAAPTPAQVTETAVVHEEKTTPALAEQAPAATQNVANAAPTRAPVAAPVPA
                     PAARANGVPKVAPFSLPLGELESLAQTAGLQWVNSDADKIAAVQAAIAAEPRPIHVPR
                     ERPPLVELDEGPLVLVETRKDLRQVTLPFEQQGGAQPH"
     gene            complement(1287..1697)
                     /locus_tag="PKV30_11695"
     CDS             complement(1287..1697)
                     /locus_tag="PKV30_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30908.1"
                     /translation="MCSDLFSAASLALISDEMKFFRLATSCRSVSISCLRVQRLLAVG
                     ARDQVLRAQPDQTGCQQRGAGTQADQEWFEPGQQARIDFKRARLLGRDDENGGSCDAR
                     GQKTVNDRTGHGTARKTPHDSQPLSLHLKRRLGA"
     gene            1700..2107
                     /locus_tag="PKV30_11700"
     CDS             1700..2107
                     /locus_tag="PKV30_11700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL30909.1"
                     /translation="MASCKTTCRRATSTSNPCPSSRPQGGYGNLVEVAHPHGYNTLYA
                     HLSRVEVTKGQQLAAGDTVGLLGSTGRSTGPHLLRSAAARRTARPGAFSGVGRHRRLG
                     LTLTSETAPCSAPAKTRPRSAPSTARRPSRSTP"
     gene            2032..2484
                     /locus_tag="PKV30_11705"
     CDS             2032..2484
                     /locus_tag="PKV30_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRL30910.1"
                     /translation="MFGSSKDKTPIRTIDSAAAKQVDTVIAEQCTLEGDLTTKNSVKV
                     DGRIQGTLRAEGRAIIGETGVVKGDVHAVDLLVLGRLEGNVHAQRLHLQASAQIHGNI
                     EAETLQVDPGARYQGSVTMRDAGAAPAVLPFSPPEPAADEAASTPAKA"
     gene            2723..>3017
                     /locus_tag="PKV30_11710"
     CDS             2723..>3017
                     /locus_tag="PKV30_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRL30911.1"
                     /translation="MSLINTKVQPFKTEAFHDGKFEPVTEKNFSDGKKWSVVIFMPAA
                     FTFNCPTEIEDAADNYADFQKANAEVYIVTTDTHFSHKVWHETSDAVGKAKFYL"
BASE COUNT          570 a         1093 c          962 g          392 t
ORIGIN      
        1 ccgtggcggc cgtggtggct cgcgcaacga gcgcggcgac cgcgccagcg acgagcgccg
       61 ccaggaaggc cgcgccgaag gccagggcga agcccgcagc ggccagcgcg acggcagccg
      121 ccgcaatggc gaacgcaacg agcgcggcga ccgtggcccg cgcactgatc gccagggccc
      181 gcgcgagcgc cgcgatgcgc tgccaggcga cggcgccaac gcggcgcaga cggccgatgc
      241 agccgccctc gacaccgcca gcgacaacgc agcacgcgcc ccgcgtggcg aacgcagcga
      301 gcgcggcgaa ggtcgcaacc gccgtggcgg tgaaggccgc cgcgaccgag gcgagcgcgg
      361 tgaccgtgcc gagcgctccg accgccccga tgcaccgccg caggacgacg ccctgcgcgc
      421 cgacagcgcc atggccgccg ctgccgtgcc ggaaggcgcc gcgcccgacc acgccggtga
      481 agccggcaac ggcgaagagc gccgcgaacg ccgctcgcgc gaccgctacg gccgcgaccg
      541 gcgcgagcgc gacggcgagc gccgcgacga gccacgtgcc gatctgccgg gcgccgtcgc
      601 ggacgacggc agcgtggtcg cggcacccgc aagcgccgac ccgtcgtcgc cgcgctcgta
      661 ctttgcccgc agtggcgacg ccgcgccgac gcctgcgcag gtgactgaga ccgccgtcgt
      721 tcatgaagaa aaaacgacgc cagcgcttgc tgagcaagcg ccagcagcta cacaaaatgt
      781 agcaaacgcc gctccgacgc gcgcgccggt ggctgcaccg gtacccgcac ccgccgcacg
      841 cgccaacggc gtgcccaagg tcgcgccgtt cagcctgccg ctgggcgagc tggaatcgct
      901 ggcgcaaacc gccggcctgc agtgggtgaa ttccgacgcc gacaagatcg ctgccgtgca
      961 agccgccatc gccgccgagc cgcgcccgat ccatgtgccg cgcgagcgcc cgccgctggt
     1021 cgagctggac gaaggcccgc tggtgctggt tgaaacccgc aaggacctgc gccaggtcac
     1081 gctgccgttc gagcagcaag gcggcgcgca gccgcactga cggtgcgcca cgccaggcgc
     1141 cgctcgcgcg ggcggcgcct ggcagaccgc atcgccggac gctctcgtta tgagagcact
     1201 ttgcgctggt cccgcttgcg ctgcggcccc attcggcata aaagaacgga cgcatccggc
     1261 caacaccgtt tcgccctcgc cctgaatcaa gccccaagcc gccgctttag gtgtaggctc
     1321 aaaggttgcg aatcgtgggg tgtctttcgt gcagtaccgt gcccagtcct gtcattcacc
     1381 gtcttctgtc cgcgcgcatc acacgatcca ccgttctcat cgtcacgccc gagcaaacgc
     1441 gctcgcttga agtcgatacg cgcctgctga cctggctcaa accactcttg gtcggcctgg
     1501 gtgccagcac cgcgctgctg acagccggtc tggtcgggct gggcgcgcag cacctgatct
     1561 cgcgcgccga cagccagcag gcgctggacg cgcaagcaag agatcgacac gctgcggcag
     1621 gaggtggcca acctgaagaa cttcatctcg tccgagatca acgccaagct ggcggcgctg
     1681 aaaagatccg agcacatgat tggcgagctg caaaactacc tgcaggcgcg cgacgtcaac
     1741 gtcaaacccg tgtccatcga gccgcccaca aggcggctac ggcaacctgg tggaagtggc
     1801 gcacccgcac ggctacaaca cgctgtatgc gcacttgtcg cgcgtcgaag tcaccaaggg
     1861 ccagcaactg gcggccggcg atacggtcgg cctgctcggc tccaccggcc gctcgaccgg
     1921 cccacacctg ttacgaagtg cagcggcgcg gcgaacggct cgacccggag cattttctgg
     1981 cgttgggcga caccggcgcc tcggcctcac gctgacatcg gagacagcgc catgttcggc
     2041 tccagcaaag acaagacccc gatccgcacc atcgacagcg cggcggccaa gcaggtcgac
     2101 accgtgatcg ccgagcagtg cacgctcgaa ggcgatttga cgaccaagaa ctcggtcaag
     2161 gtcgatggcc gcattcaggg caccttgcgt gccgagggcc gcgccatcat tggcgagacc
     2221 ggcgtggtca agggcgacgt gcacgccgtt gacttgctgg tgctggggcg cctggaaggc
     2281 aacgtgcacg cccagcgcct gcacctgcag gccagcgcgc agatccacgg caacatcgag
     2341 gccgagacgc tgcaggtcga ccccggcgcg cgctaccagg gcagcgtgac catgcgcgat
     2401 gcaggcgcgg cgcctgcagt gctgcccttc tcgccgccgg agcccgcagc cgacgaagct
     2461 gcgtccacgc ccgccaaggc ttgaggcgct gcccggacgt ccgcccgcat ccattcgatg
     2521 cgagtgcggc cattgcgtcc ggacaccggc ccgtcacggt ttctgcagta agctgattgg
     2581 ctgtcgacac cagcgtgccg gcaatgagca acgcgcctat agccgccgcc taaccataga
     2641 ttagacaaaa tgaatcacaa gttcgatagc tctaacctat aattcaccca tcgcaagttc
     2701 atcaaccacc aggagcacac gcatgtccct catcaacacc aaagtccagc ccttcaagac
     2761 cgaagccttc cacgacggca agttcgagcc ggtgaccgag aagaactttt ccgacggcaa
     2821 gaagtggtcc gtggtgatct tcatgccggc cgccttcacc ttcaactgcc cgaccgaaat
     2881 tgaagacgcg gccgacaact acgccgactt ccagaaggcc aatgccgagg tctacatcgt
     2941 caccaccgac acgcatttca gccacaaggt gtggcacgaa acgtccgacg ccgtcggcaa
     3001 ggccaagttc tacctgg
//
LOCUS       DAOMVO010000294         3004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9653_length_3004_cov_15.579858, whole genome shotgun sequence.
ACCESSION   DAOMVO010000294 DAOMVO010000000
VERSION     DAOMVO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_3004_cov_15.579858"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="moaC"
                     /locus_tag="PKV30_11715"
     CDS             <1..371
                     /gene="moaC"
                     /locus_tag="PKV30_11715"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRL30912.1"
                     /translation="LALIESGSAKKGDVLGIARIAGIMAAKKTSELIPLCHPLALSRV
                     AIDFEAARADGVSAGAVICTATVETTGPTGVEMEALTAVQVALLTIYDMCKAADRGMV
                     MTGVRLLQKHGGKSGSYVAG"
     gene            complement(645..2225)
                     /locus_tag="PKV30_11720"
     CDS             complement(645..2225)
                     /locus_tag="PKV30_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HRL30913.1"
                     /translation="MPFNLFCAGLVALPWLWPFTSGPVAATLPYLAAMAVAAVLLLAW
                     PARASGRDAAIRAVAAGWLAAALASSGIALLQYFDLETPLFPWVNIAEPGQAFGNLRQ
                     PNQLASLLVIGLWALRWWLQRGGIGRGVAIWMAAFMSTALAATASRVGLVEFLALGAL
                     ALWWAARAGGRWRVAALAVLGALALYALAAVALPWALQASEGAAGRDLVERLRSAEST
                     CGSRFILYSNVLHLISLKPWTGWGWGELAYAHYITLYDGARFCHILDNAHNLPLHLAV
                     ELGVPLAVAVCGALAWLVWRGRPWAEQNPTRQLAWGVLTVIGIHSLVEYPLWYGPFQM
                     AVVLCVWMLWSTRQERGGDEVLRRAQDERGAGAALPASTWRYPVAAAMLTMAAYAGWD
                     YHRISQIYLPVEQRAAAYRDDAMGHARKSWLYADVVRFAEVTTRPAVRENAQWMLPAA
                     LKTLHFSPEPRVIQKVIESATLLGRDDVALAHLARFRAAFPREQKQWAEDNRRVLEGA
                     QTQLRAASEAADLNEKGR"
     gene            2330..2902
                     /locus_tag="PKV30_11725"
     CDS             2330..2902
                     /locus_tag="PKV30_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRL30914.1"
                     /translation="MYPLERLGVEAYLAGEQRTEVKHEYLDGQIVAMGGASRAHGLLA
                     LSLSALLLPHARRKGCQLFTADMKVRIDHDSGSWFYYPDLVLACDPSDREPLYVRRPC
                     LLVEVLSPSTEHIDTREKLLAYRLLPSLREYLMLRQDSLQADLYQRAADGRWQHQRLT
                     APGDVLTLECLGANVTLSEIYVDLADLSVG"
BASE COUNT          534 a         1071 c          951 g          448 t
ORIGIN      
        1 cgctggcgct gatcgaatcg ggcagcgcca agaagggcga cgtgctcggc atcgcgcgca
       61 tcgccggcat catggcggcc aagaagacga gcgaactgat cccgctctgc cacccgctgg
      121 cgctgtcgcg cgtcgctatt gatttcgaag ctgctcgcgc cgatggggta agcgctggcg
      181 ccgttatttg caccgccacg gtggaaacca ccggccccac cggcgtcgag atggaagcgc
      241 tgacggcggt gcaggtggcg ctgctgacca tctacgacat gtgcaaggcc gccgaccgcg
      301 gcatggtgat gacgggcgtg cggctgctgc aaaagcacgg cggcaagtcg ggcagctacg
      361 tggcggggtg atggcggcgc tgcagggcga tggttcaaga gtgcctgaac atcacccctc
      421 ctcggaagcg tgccgggata ggttctccct ctcccgcgcg cgggagaggg ttggggtgag
      481 agtgtgcaac cgttattgag taacgtctga cgctgcctgc ggcagcgccc ctcaccccca
      541 ccttctaccc ctttcaggtg gagagggagt tcttttgctt gcgcgtccga tccttatggc
      601 ggataaatca aaaatgatag ctgctaacgc ttgtgtatca agcgctagcg gcctttttca
      661 ttcaaatctg ccgcctcgct ggccgctcgc aactgggtct gcgcaccctc cagcacgcgg
      721 cggttgtcct ccgcccactg cttctgctca cgcgggaacg ccgctcgaaa gcgcgccaga
      781 tgcgccagcg ccacgtcgtc gcggcccagc agcgtcgcgc tttcgatcac cttctggatc
      841 acgcgcggct cgggcgaaaa atgcagggtt ttcagcgctg ccggcagcat ccattgggcg
      901 ttctcgcgca ccgccgggcg cgtcgtcacc tcggcaaagc gcaccacatc ggcgtacagc
      961 cacgacttgc gcgcgtggcc catggcgtcg tcgcggtaag cggcggcgcg ctgctcgacg
     1021 ggcaggtaaa tctggctgat gcggtggtag tcccagccgg cataggcggc catcgtcagc
     1081 atcgcggcgg cgacggggta acgccaagtg ctggccggca aggcggcgcc cgcgccccgt
     1141 tcgtcctgag cccgtcgcag gacttcatcc ccgccgcgtt cctgccgcgt cgaccacagc
     1201 atccatacgc acagcaccac cgccatctgg aacggcccgt accacagcgg gtactccacc
     1261 agactgtgga tgccgatcac cgtcagcacg ccccaggcca gctggcgcgt ggggttttgc
     1321 tcggcccacg gccgcccgcg ccagaccagc cacgccagcg caccgcacac cgccaccgcc
     1381 agcggcacgc ccagctcgac cgccaagtgc agcggcaggt tgtgcgcgtt gtctaggatg
     1441 tggcaaaagc gcgcgccgtc gtacagagtg atgtagtgcg cgtacgccag ctcgccccag
     1501 ccccagcccg tccaaggctt caggctgatg agatgcaaga cattgctata aagaatgaag
     1561 cggctgccgc aggtggattc ggcgctgcgc agccgttcga ccagatcgcg tccggccgcg
     1621 ccctcgctcg cctgcaaggc ccacggcagc gccaccgccg ccagtgcgta cagcgcgagt
     1681 gcgcccagca ccgccagcgc cgccacgcgc cagcgcccgc ccgcgcgcgc ggcccaccac
     1741 aaggccagag cgcccagcgc caggaactca accagcccca cgcgcgaagc cgtggccgcc
     1801 aacgccgttg acatgaacgc cgccatccag atcgccacgc cgcggccaat gccgccgcgc
     1861 tgcagccacc accgcagagc ccacagcccg atcaccagca gagaagccag ctggttgggc
     1921 tgccgaaggt tgccgaacgc ctgtccaggc tcggcgatgt tcacccaggg aaaaagtggc
     1981 gtttcgagat cgaaatactg caacagcgcg atgccgctgc tggccagcgc cgccgccagc
     2041 cagccggcgg cgaccgcgcg aatggcggca tcgcgccccg aagcccgtgc gggccacgcc
     2101 agcagcagca cagccgccac cgccatggcc gccagatacg gcagcgtggc cgccacgggg
     2161 ccggaggtga aaggccagag ccagggcagc gccaccagtc cggcgcaaaa gagattgaac
     2221 ggcatggcgt cgattgtgcc cagcctgctg ctccgcccgg cttgggcaaa cgagcgccgc
     2281 cgcctacact gacgacttag cccccgaagg actgcccaag aacgccgcca tgtatccgct
     2341 cgaacgcctc ggtgtcgaag cctatctcgc gggtgaacag cgcaccgaag tcaagcacga
     2401 atacctcgac ggccagatcg tcgccatggg cggcgccagc cgtgcgcacg gcctgctggc
     2461 cctctcgctc agcgccctgc tgctgcccca cgcccgtcgc aaggggtgcc aactgttcac
     2521 agccgacatg aaggtgcgca tcgaccacga cagcggcagc tggttctatt accccgacct
     2581 ggtgctggcc tgcgacccca gcgaccgcga gccgctgtac gtgcgccgcc cctgcctgct
     2641 ggtggaagtg ctttcgccca gcaccgagca catcgacacg cgcgaaaaac tgctggccta
     2701 ccgcctgctg cccagcctgc gtgaatacct gatgctgcgc caggacagcc tgcaagccga
     2761 cctgtatcag cgtgccgctg atggccggtg gcagcaccaa cgcttgaccg cgccgggcga
     2821 cgtgctcacg ctggaatgcc tgggtgctaa tgtcacgctg agcgagattt atgtcgacct
     2881 ggcggatttg agcgtcgggt aatcccgggt gggcggacca aaaatcgtga tctgacccca
     2941 tttttacctc cctgcacgcc gagctgtacc agcgccgcga cgatggcggc tggacgcacc
     3001 agcg
//
LOCUS       DAOMVO010000295         3004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9654_length_3004_cov_10.544591, whole genome shotgun sequence.
ACCESSION   DAOMVO010000295 DAOMVO010000000
VERSION     DAOMVO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9654_length_3004_cov_10.544591"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKV30_11730"
     CDS             complement(<1..576)
                     /locus_tag="PKV30_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30915.1"
                     /translation="MTSDQLPRPTDDELVARYHEATALEDAAPGPDLRDAILAQAQVL
                     AWKHAETMADDGEPAIDEVAAGARPASAEPVFHSELPPPPALAVEAANDRRWFVPAVA
                     SVAVLGLAGLLALQFGHAPADDQDVALGRAPAPQAEPAAPAAPAAADVEAKSAAADQA
                     TAAAPAAEPARPAEAPSTDAPAPPPSVAAPPA"
     gene            complement(737..1336)
                     /locus_tag="PKV30_11735"
     CDS             complement(737..1336)
                     /locus_tag="PKV30_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL30916.1"
                     /translation="MSPLAATADEQLMRAYATGQAAAFETLYDRHSARVWRYILRSTG
                     DAAAADDLAQEVWFSVAREAGRYAPRASAPDLPLARFTTWLFTLARHRVIDHLRARRP
                     QASLDAPVGEGDDTLADTLAAPSGFGPLRRIETRQQAAQLLAALAELPPEQREAFLLQ
                     AEAEMSVSDIAVATGVGFETAKSRLRYARAALRRALENL"
     gene            complement(1398..2219)
                     /locus_tag="PKV30_11740"
     CDS             complement(1398..2219)
                     /locus_tag="PKV30_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30917.1"
                     /translation="MKSLPRLMMSALALSAATLAAPAQAIGRLVDVDVIDRDTGAQLP
                     VYQHRGEWWIAGRPGARYAIQVRNAAPGRVLGVMAVDGVNVISGDTASWEQTGYVLSR
                     GQTAQITGWRKSDAEVAAFHFTALPNSYAARTGRPDDVGVIGVAVFRERYVPPPPPVS
                     AAPWPSRREGKFQGEPSATAAESAAPQADASARERAAAPAPRLGTGHGERETSYVSNT
                     SFERRSSRPDEVITIRYDSRQNLIAAGIIANPVQQPVPRPFPESVRYTPDPPAWR"
     gene            2357..2761
                     /locus_tag="PKV30_11745"
     CDS             2357..2761
                     /locus_tag="PKV30_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30918.1"
                     /translation="MKTPDDTPQLTAPELYTGEDGRARFRERPLPLAEGTPMTRLSGL
                     APSSGWQFRRSPVGFASDVHCTTAPQWLVVLQGQMEIGLPHDGSTRVFGPGEFFYSND
                     TLPAGAVFDPRVHGHSSRLLGDAPLVTLFVRT"
BASE COUNT          396 a          999 c         1141 g          468 t
ORIGIN      
        1 ggcgggcggc gcggcgaccg atggcggcgg cgcgggcgcg tcggtgctgg gtgcttcggc
       61 agggcgagcc ggctcggctg caggcgcggc ggcggtggcc tgatcggcgg cggctgactt
      121 ggcttcaacg tcggccgcag cgggcgcggc cggcgctgcg ggctcggctt gcggcgcggg
      181 ggcgcggccc agtgccacgt cctggtcgtc agccggcgcg tggccgaatt gcagcgccag
      241 caggccggcc aggcccagca cggcgacgct ggccaccgcc ggcacgaacc agcggcggtc
      301 gttggcggct tcgacggcga gcgctggggg cggtgggagt tctgaatgaa aaaccggttc
      361 agcgcttgca ggacgtgcgc cagcagctac ctcatcgata gcaggctcgc cgtcgtcggc
      421 catcgtttcg gcgtgcttcc aggccagcac ctgcgcctgc gcgaggatgg catcgcgcag
      481 gtcggggccg ggcgccgcgt cttccagcgc cgtcgcctcg tggtagcggg cgaccagctc
      541 gtcgtcggtc gggcgtggca gctgatcgga ggtcatcgcg gtgctcctcg ttgccgtctg
      601 tcagcccgcg cccaccctct ggtggccgcg gagcaggcca cgcgcgaaac gccgcggacg
      661 gacctgcgcc ggccgctggc gttgtccccc ctcccgcagg agagggggga aggcgcgaag
      721 cgactcaggg gggggctcat aaattttcca gtgctcgccg cagcgccgcg cgggcgtagc
      781 gcaggcggct tttggcggtc tcgaagccga cgccggtggc gacggcgatg tcgctcacgc
      841 tcatttcagc ctcggcctgc agcaagaagg cttcgcgctg ctcgggcggc agttcggcca
      901 gcgcggccag cagttgcgcg gcctgctggc gcgtttcgat gcgccgcagc gggccgaagc
      961 cggagggcgc ggccagcgtg tcggccaggg tgtcgtcgcc ctcgcccacc ggtgcgtcca
     1021 ggctggcctg cgggcggcgc gcgcgcaggt ggtcgatgac gcggtggcgc gccagcgtga
     1081 acagccaggt ggtgaagcgc gccagcggca ggtcgggtgc cgaggcgcgc ggcgcgtagc
     1141 gcccggcctc gcgcgccacg ctgaaccaca cctcctgcgc caggtcgtcg gccgcggcgg
     1201 cgtcgcccgt gctgcgcaaa atgtagcgcc acacgcgcgc cgaatgccgg tcgtacagcg
     1261 tctcgaacgc cgcggcctgg ccggtcgcgt aagcgcgcat gagctgctca tcggcggtgg
     1321 cggccagcgg ggacatggcc gcgattatgt cggtgcgccg ccatgcgccc ctcatccgcc
     1381 gggggcacgg cgcccgatca gcgccacgcc ggcgggtccg gcgtgtagcg caccgattcc
     1441 ggaaacggcc gcggcaccgg ctgctgcacc gggttggcga tgatgccggc ggcgatcagg
     1501 ttctggcggc tgtcgtagcg gatggtgatg acctcgtccg gccggctgct gcgccgctcg
     1561 aagctggtgt tgctgacgta cgaggtttcg cgctcgccgt ggccggtgcc caggcgcggg
     1621 gcgggcgcgg cggcgcgttc gcgggcgctg gcgtcggcct gcggggcggc ggattcggcg
     1681 gcggtggccg atggttcgcc ctggaacttg ccttcgcgcc gcgacggcca cggcgctgcc
     1741 gacaccggcg gtggcggcgg cacatagcgc tcgcggaaca ccgcgacgcc gatcacgccg
     1801 acgtcatcgg gccggccggt gcgcgcggca tacgaattgg gcagcgcggt gaagtggaag
     1861 gccgccactt cggcgtcgct cttgcgccag ccggtgatct gcgcggtttg cccgcgcgaa
     1921 agcacgtagc cggtttgctc ccacgacgcc gtgtcgccgc tgatgacgtt gacgccatcc
     1981 accgccatca cgcccagcac gcggcccggc gcggcgttgc gcacctggat cgcgtagcgc
     2041 gcgccggggc gcccggcaat ccaccattcg ccgcgatgct ggtacaccgg cagctgggcg
     2101 ccggtgtcgc ggtcgatcac gtcgacatcg accagacggc cgatggcttg cgcgggggca
     2161 gccagcgtgg cggcggacag ggccagcgcg gacatcatca agcggggcag cgatttcatg
     2221 ggcatctcca tcaatgaaag ggtggtgcgg catgaaacgg gcgtggccgg ccgatggggt
     2281 tgacggcggc gcataaagat gtgtcggctg gtaacgcgct acgcttggcg ccttgttgct
     2341 gaacaccgcg ctggccatga agacccctga cgacaccccc caactcaccg cgcccgagct
     2401 gtacaccggc gaagacggtc gcgcgcgctt tcgcgagcgg cccctgccgc tggccgaagg
     2461 cacgccgatg acgcgcctgt cggggctggc gccttcaagc ggctggcagt ttcggcgcag
     2521 cccggtcggc tttgccagcg acgtgcactg caccaccgcg ccgcagtggc tggtggtgct
     2581 gcaggggcag atggaaatcg gcctgccgca cgacggcagc acgcgcgtgt tcggtccggg
     2641 cgagtttttc tattcgaacg acacgctgcc ggcgggcgcc gtgttcgacc cgcgcgtgca
     2701 tggccattcc agccgcttgc tgggcgatgc gccgctggtg acgctgttcg tgcggacgtg
     2761 agccgaactg gcgattgcta ttcatttgat agctgcttgc gcacgtagat cgggcgctgg
     2821 agcctgattt gacttgattg gccctgaatc gcaggtgaac atcgttgctt acccgtcatt
     2881 ccggcgaaag ccggaatcca agggcgtctc cacaaaaaat cgcctgtgga aatgacaatc
     2941 gccgtctgga ttccggcttt tgccggaatg acggtggcag gcaggattta gtgggttttc
     3001 tggt
//
LOCUS       DAOMVO010000296         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9699_length_2995_cov_15.931633, whole genome shotgun sequence.
ACCESSION   DAOMVO010000296 DAOMVO010000000
VERSION     DAOMVO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_2995_cov_15.931633"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..516
                     /locus_tag="PKV30_11750"
     CDS             58..516
                     /locus_tag="PKV30_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRL30919.1"
                     /translation="MLGIKRQPATRSVIGAGMRVQGPCTFSDGLQIDGEVLGDVTAEG
                     GQPSVLVIGEGGSVQGAISADHVVIGGSVVGPVLARELLELQSKARVQGDVRYKTLEM
                     QQGAVIAGQLQPQLTPPPAAARESQAQEPTDVRSPPPAEPTEPTLDFDRP"
     gene            619..996
                     /gene="erpA"
                     /locus_tag="PKV30_11755"
     CDS             619..996
                     /gene="erpA"
                     /locus_tag="PKV30_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRL30920.1"
                     /translation="MSAVAQDITTPTAMPDPIVFTDSAAAKVADLIAEEGNPELKLRV
                     FVQGGGCSGFQYGFTFDEIANDDDTTMTKNGVSLLIDAMSYQYLVGAEIDYKEDLQGA
                     QFVIKNPNAQSTCGCGSSFSVPE"
     gene            complement(1136..2494)
                     /locus_tag="PKV30_11760"
     CDS             complement(1136..2494)
                     /locus_tag="PKV30_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL30921.1"
                     /translation="MKQELISTGLAFADFVHRHPKRISAAIAVLLMGGAGGAFAVASL
                     APTAPTEPLRLVSESVAPSALTQQADALDVHSFTLYRSEQTRASDTPEALLKRLGLAD
                     PAAAAFLRKNETTRQALFGKAVAGRTVTAEATDRLELQTLRTRWVESADDDSFKRLVV
                     EKSGDSFSVRIETAPLVANERLAGATIRGTLYAATDEAGLPDSVTKQLTEIFESNVDF
                     HRGLRRGDRFSIVYETLEADGEPLRAGKILSAEVVNRGKTHQAIWFKEAGASKGTYYS
                     FDGQSLRKAYLLSPMEVSRITSGFGMRNHPVYGYSREHAGTDYAAPTGTPVRSIGDGS
                     VSFAGVQNGYGNVIQIKHRNGKDSTLYAHLSRIDVKVGDNVMQGENIGAVGSTGVSTG
                     PHLHFEFRINNEPQDPAEVLAEQREYVPVSPGGKAAFAKLASGMKTQLAAASEITRGS
                     FE"
     gene            2691..>2995
                     /locus_tag="PKV30_11765"
     CDS             2691..>2995
                     /locus_tag="PKV30_11765"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073020.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRL30922.1"
                     /translation="MSDKLLTNPPITDRVREAMAISLRGCDELLPQDDWLTKLARSEA
                     TGVPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTTLIGDPSGRNS
                     "
BASE COUNT          532 a          917 c         1023 g          523 t
ORIGIN      
        1 ttttttatgg ccgcgtggta aaagcgggca ttgcggaaaa aagcaggaga acgagccatg
       61 ttgggcatca agagacagcc ggccacgcgc agcgtcatcg gcgccggcat gcgggtgcag
      121 ggcccgtgca cgttttcaga cggcctgcag attgacggcg aagtgctggg cgatgtgacg
      181 gccgagggcg gccagcccag cgtgctggtg atcggcgaag gcggcagcgt gcagggcgcc
      241 atcagcgccg atcacgtggt gattggcggc agcgtggtcg gcccggtcct ggcgcgcgag
      301 ctgctcgaat tgcagtcgaa ggcgcgcgtg cagggcgacg tgcgctacaa gacgctggaa
      361 atgcagcagg gcgccgtcat cgccggccag ctgcagccgc agctgacgcc gccgccggcg
      421 gccgcgcgcg aaagccaggc gcaggagcca accgatgtac gcagcccgcc gccggcggag
      481 ccgaccgagc cgacgctgga tttcgatcgc ccttgacctc agggtggcgc ggcccgttgc
      541 gccagcgaaa tcccgagtaa ttcgatatac tatttaaaag gccctgcaga cgcaggcgcc
      601 gactgcagga gttacgccat gagcgccgtt gcgcaagaca tcaccacccc gaccgcgatg
      661 cccgatccca tcgtgttcac cgactcagcc gccgccaagg tggccgatct gatcgccgaa
      721 gaaggcaacc ctgaactgaa gttgcgcgtg ttcgtgcagg gcggcggctg ctcgggcttt
      781 cagtacggtt tcaccttcga cgaaatcgcc aacgacgacg acacgacgat gaccaagaac
      841 ggcgtctcgc tgctgatcga tgccatgagc tatcagtatc tggtgggcgc cgagatcgac
      901 tacaaggaag acctgcaagg cgcgcagttc gtgatcaaga acccgaacgc gcagagcacg
      961 tgcggctgcg gctcatcgtt ctcggtgccg gaataagcgt cgtcagctca ccacgacaaa
     1021 gggcgccttg cggcgccctt ttgtttgggt ggaatcttaa tcaaattggc cgctggcgct
     1081 tgtccagaaa gcgcgagcag ctataaattc aggagtaatc gccggctaag ccgactcact
     1141 caaagctgcc gcgcgtgatc tcgctggcgg cggccagctg ggtcttcatg ccgctggcca
     1201 gcttggcgaa ggcggccttg ccgccgggcg acacgggcac gtattcgcgc tgctcggcca
     1261 gcacctcggc cgggtcttgc ggctcgttgt tgatgcggaa ttcgaagtga aggtgcggcc
     1321 cggtggacac gccggtggaa cccacggcgc caatgttttc gccctgcatc acgttgtcgc
     1381 cgaccttgac gtcgatgcgc gacaggtgcg cgtacagcgt cgaatctttg ccgttgcggt
     1441 gcttgatctg gatgacgttg ccgtagccgt tttgcacgcc ggcaaagctg accgagccat
     1501 cgccgattga gcgcaccggc gtgccggtgg gcgcggcgta gtcggtgccg gcgtgttcgc
     1561 ggctgtagcc gtagaccggg tggttgcgca tgccaaagcc gctggtgatg cgcgagactt
     1621 ccatcggcga cagcaggtaa gccttgcgca ggctctggcc gtcgaaagag taataggtgc
     1681 ccttgctggc gccggcttcc ttgaaccaga tggcctgatg cgtcttgccg cggttgacca
     1741 cttcggcgct caggatcttg ccggcgcgca gcggctcgcc atcggcttcc agcgtttcgt
     1801 agacgatcga gaaacggtcg ccgcggcgca ggccgcggtg gaaatcgacg ttggattcga
     1861 agatttcggt cagctgcttg gtcacgctgt cgggcaggcc ggcctcgtcg gtggcggcgt
     1921 acagcgtgcc gcgaatggtg gcgccggcca ggcgttcgtt ggcgacaagc ggcgcggttt
     1981 cgatgcgcac gctgaacgaa tcgccgctct tttcgaccac caggcgcttg aagctgtcgt
     2041 cgtccgcgct ttcgacccag cgcgtgcgca gggtttgcaa ttcgagccga tcggtggcct
     2101 cggccgtcac ggtgcggccg gccacggcct taccgaacag cgcctggcgc gtggtctcgt
     2161 tcttgcgcag gaaggcggcg gccgccgggt cggccaggcc caggcgcttg agaagcgctt
     2221 cgggcgtgtc gctggcgcgc gtttgctcgc tgcggtagag ggtgaatgag tgcacgtcga
     2281 gtgcgtcggc ctgctgcgtc aatgcgctgg gcgccacact ttcagacacc aggcgcagcg
     2341 gctcggtcgg cgcggtgggc gccaacgagg cgacggcaaa agcgccgccc gcgccaccca
     2401 tcagcagaac ggcgatggcg gcagagatgc gtttgggatg gcgatggaca aagtcagcga
     2461 acgcgaggcc ggtggaaatc agttcttgct tcacgcgacg gggtttccgg aaattcgttg
     2521 cagtcgggct gacggcgcgc gaaaaaactt tgtacaacca acgcgcgcgg tgcggacagc
     2581 caagcgaagc gcccttggtc caagcgtggt gccggggcgc ggcctctaga atcgggcctt
     2641 agcctacaag tataacggcg taggcatgcg cctacaaccc aatgtatacg atgtctgaca
     2701 agttgctgac aaatccgccc atcaccgacc gcgttcgcga ggccatggcg atttcgctgc
     2761 gcggctgcga cgaattgctg ccgcaggacg actggctgac caaactggcc cgctcagagg
     2821 cgaccggcgt gccgctgcgc atcaagctgg ggctcgaccc gacggcgccc gacatccaca
     2881 tcggccacac ggtggtgctg aacaagatgc gccagctgca ggacctgggg cacacggtga
     2941 tctttttgat cggcgacttc accacgctga ttggcgatcc gtccgggcgc aattc
//
LOCUS       DAOMVO010000297         2987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9752_length_2987_cov_16.016712, whole genome shotgun sequence.
ACCESSION   DAOMVO010000297 DAOMVO010000000
VERSION     DAOMVO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9752_length_2987_cov_16.016712"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1248)
                     /locus_tag="PKV30_11770"
     CDS             complement(49..1248)
                     /locus_tag="PKV30_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRL30923.1"
                     /translation="MPTPSNRAGRYVQQATGYRAFVPEPLPPEPPIVFDGELQTWLSN
                     ADRDLARLDAIADVLPNPDLFVAMYVRHEAVLSSQIEGTQSTLEDVLAFETAATADDA
                     PQDVEEVFNYVRAMNHGLRRLAEHFPLSLRLIREIHAELMHGGGRKIRGGEKTPGEFR
                     RTQNWIGGAGCTLTTASFVPPPPHELMNALDGLEKFLHQGRHSLPLLVRCGLAHAQFE
                     TIHPFLDGNGRVGRLLITLLLCEEGALTRPLLYLSVFLKAHRAEYYDRLTAIRTHGHW
                     EAWLKFFLRGVSTTARAATQTARDIVALQAAHRAAVANNAYALKLLDHLYQQPYVNAK
                     TCAQVTGCSMPTAIKLLEDLRARTWLHETTGQQRNRVYRYQPYLDLFHRDTVQSVFET
                     QFTPEAP"
     gene            1389..1901
                     /locus_tag="PKV30_11775"
     CDS             1389..1901
                     /locus_tag="PKV30_11775"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRL30924.1"
                     /translation="MTFLHAVQADITTLAVDAIVNAANSSLLGGGGVDGAIHRAAGPE
                     LVFECRLLGGCKTGDAKTTRGHRLPAKWVIHTVGPVWRGGDQGEPALLTACYRRAMEE
                     AVRVGARTVAFPAISTGVYGYPKAAAATVAVATLRQALVAHPGAFDEVVLCCFSDDDR
                     RTVQAQLDAG"
     gene            2044..2931
                     /locus_tag="PKV30_11780"
     CDS             2044..2931
                     /locus_tag="PKV30_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRL30925.1"
                     /translation="MTPLLQSPALLLLAVGTLLGLNFPLGKLAQAAQVPAVLWAALIS
                     LGASSLLGAWLLLRRRPVVCDAQHARYFVITALVSYALPNLLVLAAMAHLGSGPTAMM
                     FTLSPLFTALLSRLAGLRAPARLEYWGIAIGFGGALLVALARGEAGRPAAWLWVGVGL
                     LIPLLLALGNVYRSLDWPPRADGTWLAVGSHGVAALLLLALCALTGAWRGLPALAGVP
                     WLAAAQVAAAVLMFPLFFRLQALGGPVLLSQIGTVGAGVGVAVGAGLLGERYPPLVWG
                     GVALIALGIGLTVWARRGT"
BASE COUNT          396 a          850 c         1166 g          575 t
ORIGIN      
        1 ttgggtgaag gcgtctgggc ggccgagcca gtcgagggtg ggcatgggtc agggggcttc
       61 tggcgtgaac tgggtttcaa acacgctttg cacggtgtcg cggtgaaaca ggtcgaggta
      121 gggctggtaa cggtacacgc ggttgcgctg ctggccggtg gtttcgtgca gccaggtgcg
      181 cgcgcgcagg tcttcgagca gtttgatggc ggtgggcatg ctgcagccgg tgacctgggc
      241 gcaggtcttg gcgttgacgt agggctgctg atacaggtgg tcgagcagtt tcagcgcgta
      301 ggcgttgttg gccaccgcgg cgcggtgcgc ggcttgcagg gcgacgatgt cgcgcgcggt
      361 ctgggtggcg gcgcgcgcgg tggtgctgac gccgcgcaga aagaacttga gccacgcctc
      421 ccagtggccg tgcgtgcgga tggcggtgag gcggtcgtag tattcggcgc ggtgcgcctt
      481 gagaaatact gacaagtaca gcagcgggcg cgtgagggcg ccttcctcgc acagcagcaa
      541 ggtgatgagc aggcggccga cgcggccgtt gccgtcgaga aacgggtgga tcgtttcgaa
      601 ctgcgcgtgc gccagtccgc agcgcaccag caagggcagg ctatggcggc cttgatgcaa
      661 gaacttttcc agaccatcaa gcgcattcat cagctcgtgc ggcggcgggg gcacgaagct
      721 ggcggtggtc aaggtgcagc cggcgccgcc gatccagttt tgcgtgcggc ggaattcgcc
      781 gggggttttc tccccgccgc gaatcttgcg gccaccgccg tgcatcagtt cggcgtggat
      841 ttcgcgaatc aggcgcagac tcaaggggaa gtgctcggcc aggcgccgca agccgtggtt
      901 catggcgcgc acgtagttga agacttcttc cacatcttgc ggcgcgtcgt cggcggtggc
      961 agcggtctcg aatgccagca cgtcttcaag cgtgctttgc gtgccttcga tctgcgacga
     1021 caacacggct tcgtgccgca catacatggc gacgaacagg tcggggttgg gcagcacatc
     1081 ggcgatggcg tcaagccgcg ccaggtcgcg gtcggcgttg gagagccagg tttgcagctc
     1141 accatcgaaa acgatcggcg gttcgggcgg cagcggctcg ggcacgaagg cgcggtagcc
     1201 agtggcctgc tggacgtaac ggccggcgcg gttggatggg gtgggcatgg ggtgcgttta
     1261 aataggagcg cgctgggttg tattttattt aaaaaaaaga ctcaattttt aatagtagga
     1321 gtccgaaggc caggctgttt aagttgtgcg ggcaaaaaga ctgtgtttat gctcatggtt
     1381 gatcaagtat gacgtttttg cacgctgttc aagccgacat caccacgctg gcggtggacg
     1441 ccatcgtcaa cgccgccaac agcagcctgt tgggcggcgg cggggtggat ggggccattc
     1501 accgcgcggc ggggccggag ctggtgttcg aatgccggct gctcggcggt tgcaagacgg
     1561 gcgacgccaa gacgacgcgc ggccatcgcc tgccggccaa gtgggtgatt cacaccgtgg
     1621 gcccggtgtg gcgtggtggc gatcagggcg agccggcgct gctgaccgcg tgttaccgcc
     1681 gcgcgatgga agaggcggtg cgtgtcggtg cgcgcacggt ggcgtttccg gccatcagca
     1741 cgggggtgta cggctacccc aaggcggctg ccgcgaccgt ggcggtggcg acgctgcggc
     1801 aggcgctggt ggcacatccg ggcgcgttcg acgaagtggt gctgtgctgt ttttcagacg
     1861 acgaccggcg caccgtgcag gcgcagttgg atgcggggtg acgcgcgccg cgcgcctgtc
     1921 gccagtcaaa tgcttcaaat ttggtagctg cttgcgcttg ccctgcttgc gctggctgcc
     1981 ctttatcttc gatatcctgt gcgcatcgcg ctgccagcgc ccccggtttg ctgatccgcc
     2041 atcttgacgc cgctcctcca atcacccgcc ctcctgctgc tggccgtcgg cacgctgctg
     2101 ggcctgaact ttccgctcgg caagctggct caagccgcgc aggtgccggc ggtgctgtgg
     2161 gcggcgctga tttcgctggg cgccagctcg ctgctgggcg cgtggctgct gctgcgtcgc
     2221 cgaccggtgg tgtgcgatgc gcagcacgcg cgctacttcg tcatcacggc gctggtgtcg
     2281 tacgcgctgc ccaatctgct ggtgttggcg gccatggcgc acctgggcag cgggccgacg
     2341 gcgatgatgt tcacgctgtc gccgctgttc accgcgctgc tgtcgcgcct ggccgggctg
     2401 cgcgcgccgg cgcggctgga gtactggggc atcgccatcg gctttggcgg cgcgctgctg
     2461 gtggcgctgg cgcgtggtga ggcggggcgg cctgccgcgt ggctgtgggt gggcgtgggg
     2521 ctgctgattc cgctgttgct ggcgctgggc aacgtgtacc gctcgctcga ctggccaccg
     2581 cgcgccgacg gcacctggct ggcggtgggc agtcacggcg tggcggcgtt gctgctgctg
     2641 gcgctgtgtg cgctgacggg cgcctggcgc ggcctgccgg cactggcggg cgtgccgtgg
     2701 ctggcggcgg cgcaggtggc ggcggcggtg ctgatgtttc cgctgttctt tcggctgcag
     2761 gcgctgggcg ggccggtgct gctgagccag atcggaaccg tgggcgcggg cgtgggcgtg
     2821 gcggtgggcg cgggcctgct gggcgagcgc tatccgccgc tggtgtgggg cggcgtggcg
     2881 ttgatcgcgc tgggcatcgg gctgacggtg tgggcgcggc gcgggacttg aaggggttga
     2941 aatgctattg atgtgggagc tgcttgcgca ggtgggacaa gcgctgg
//
LOCUS       DAOMVO010000298         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9792_length_2977_cov_13.648186, whole genome shotgun sequence.
ACCESSION   DAOMVO010000298 DAOMVO010000000
VERSION     DAOMVO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9792_length_2977_cov_13.648186"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PKV30_11785"
     CDS             <1..1583
                     /locus_tag="PKV30_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRL30926.1"
                     /translation="NVMPALYKGLAIAGVLSLIAFYFVTVWLMPDNAIRDSGTQIRLF
                     GACAVGLILTGALVWITEYYTGTQYKPVRHIAQASTTGHGTNIIAGLGVSMRSTAWPV
                     LMVCVAIIASYSLAGLYGIAVAATSMLSMAGIVVALDAYGPITDNAGGIAEMSELPSS
                     VRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLEGLGSAISFDLSDPMV
                     IVGLFIGGMIPYLFGAMAMEAVGRAAGSVVVEVRRQFSTIKGIMEGTAKPEYGKAVDM
                     LTSAAIKEMVIPSLLPVVVPILVGLLLGPKALGGLLMGTIVTGLFVAISMCTGGGAWD
                     NAKKYIEDGHHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPLV
                     VKFHGGEVKAAPHAPVAATAPAGAMADADSVQVEGNVVRFYFATGKAAPHPDGARALQ
                     VIVDGVKAGKKAIVSGYVDATGSAAANEEISKQRAFAVRDLIKSLGVADEQIDLRKPD
                     NIQAGTGAQARRVEVTLQ"
     gene            1774..>2977
                     /locus_tag="PKV30_11790"
     CDS             1774..>2977
                     /locus_tag="PKV30_11790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRL30927.1"
                     /translation="MNAPVTLPPPAETETPDTLAATANSLAAGTRLGEFELRGVLGEG
                     GFAIVYKAHDKSLRRTVAIKEYMPGAIATRTPDGSVVPREASVAGTLQRGLDSFLDEA
                     RLLAQFDHPALIRVYRFWQQNNTAYMAMQLCQGRTLRALREEQPKLVGSEAWLKQMLA
                     PILDALELLHAQRCYHRDISPDNIMILDSGVPMLLDFGAARQAMGDATQALTVIVKPG
                     YAPIEQYDSVLEQGPWTDVYSLGAVLHYAITGRPVTASVSRLLKDPLPRLADTPGLAI
                     SPAFARAIDRALTVHPDERIRSITEFREALELPTFWAEMQRQSVELGALRSHSGHTGF
                     GTLDGLEDEVAPSKAEPVDAPVDAEAEAARADIERRRRERARRQAAGQGRARDEGARA
                     GLHQGTGKT"
BASE COUNT          504 a         1038 c          989 g          446 t
ORIGIN      
        1 agaacgtgat gcccgcgctg tacaagggcc tggcgattgc gggcgtgctg tcgctgatcg
       61 ccttctactt tgtcaccgtg tggctgatgc ccgacaacgc catccgggac agcggcacgc
      121 agatccgcct gttcggcgcg tgcgcggtgg gcctcatcct gaccggcgcg ctggtctgga
      181 tcaccgagta ctacaccggc acgcaataca agccggtgcg ccacatcgcg caggccagca
      241 ccaccggcca cggcaccaac atcattgccg gcctgggcgt gtcgatgcgc tccaccgcct
      301 ggccggtgct gatggtgtgc gtggccatca ttgcgtccta ctcgctggcc ggcctgtacg
      361 gtatcgcggt ggccgccacg tcgatgctct ccatggccgg tatcgtggtg gctctggacg
      421 cctacggccc catcaccgac aacgccggcg gcattgccga gatgagcgag ctgcccagca
      481 gcgtgcgcga catcaccgac ccgctggacg ccgtgggcaa caccaccaag gccgtcacca
      541 agggctacgc catcggttcc gccggcctgg ccgcgctggt gctgtttgcc gactacacgc
      601 acaagctcga aggcctgggc agcgccatca gctttgattt gagcgacccg atggtgatcg
      661 tcggcctgtt catcggcggc atgattccct acctgttcgg cgccatggcg atggaagccg
      721 tgggccgcgc ggccggcagc gtggtggtgg aagtgcgccg ccagttcagc accatcaagg
      781 gcatcatgga aggcacggcc aagcccgagt acggcaaggc ggtggacatg ctgacatcgg
      841 ccgccatcaa ggaaatggtc attcccagcc tgttgcccgt ggtggtgccg atcctggtcg
      901 gcctgctgct cggccccaag gcgctgggcg gcctgctgat gggcaccatc gtcaccggcc
      961 tgttcgtcgc catcagcatg tgtaccggcg gcggcgcgtg ggacaacgcc aagaagtaca
     1021 tcgaggacgg ccaccacggc ggcaagggat ctgaggcgca caaggccgcc gtcaccggcg
     1081 acaccgtggg cgacccttac aaggacaccg ccggcccggc cgtcaacccg ctgatcaaga
     1141 tcatcaacat cgtggcgctg ctcatcgtgc cgctggtggt caagttccac ggcggcgagg
     1201 tgaaggcggc accgcacgcg cccgtggcgg ccacggcccc ggccggcgcc atggcagacg
     1261 ccgattcggt gcaggtcgaa ggcaacgtgg tgcgctttta cttcgccacc ggcaaggccg
     1321 cgccgcaccc cgatggcgcg cgcgcgctgc aagtcatcgt cgatggcgtg aaggcgggca
     1381 agaaagccat cgtcagcggc tatgtcgacg ccaccggcag cgccgccgcc aacgaggaaa
     1441 tctccaagca gcgcgccttt gccgtgcgcg acctgatcaa gtcgctgggc gtggcggacg
     1501 agcagatcga tctgcgcaag cccgacaaca tccaggccgg caccggcgcg caggcgcggc
     1561 gcgtggaagt caccttgcaa taacagaaaa gcgcgcccgg cgcgctcacc cccctgtgtg
     1621 caggcccgcc ttcgtgcggg ctttttcatg cgcgcctgcg catgacatag ttcacaaaaa
     1681 ttactcccta agcatacatt ttcacgagcg attggcctac gcttgcacct gcgcggcgcc
     1741 cccaacatgc gccagccgac ttcgacttat tgcatgaacg cccctgtcac actgccgccg
     1801 cccgccgaga ccgaaacgcc cgatacgctg gccgccaccg ccaacagctt ggctgcgggc
     1861 acgcggctgg gcgagttcga actgcgcggc gtgctgggcg aaggcggctt tgccatcgtc
     1921 tacaaggcgc acgacaagtc gctgcgccgc acggtcgcca tcaaggaata catgcccggc
     1981 gccattgcca cccgcacgcc agacggcagc gtggtaccgc gcgaggcgtc ggtcgccggc
     2041 acccttcagc gcgggctgga cagctttctg gacgaggcgc gcctgctggc gcagttcgac
     2101 cacccggcgc tgatccgcgt ctaccgcttc tggcagcaga acaacaccgc ctacatggcc
     2161 atgcagctgt gccagggccg cacgctgcgc gcgctgcgtg aagagcagcc caagctggtc
     2221 ggcagcgagg cctggctcaa gcagatgctg gcgccgatcc tcgacgcgct ggagttgctg
     2281 cacgcgcagc gctgctatca ccgcgacatc tcgcccgaca acatcatgat cctggactcg
     2341 ggcgtgccga tgctgctcga cttcggcgcc gcgcgccagg ccatgggcga tgccacgcag
     2401 gcgctgacgg tgatcgtcaa gcccggctac gcgccgatcg agcagtacga cagcgtgctc
     2461 gagcaaggcc catggacgga tgtgtacagc ctgggcgcgg tgctgcacta cgccatcacc
     2521 ggccggccgg tcacggcctc ggtctcgcgc ctgttgaaag atccgctgcc gcgcctggcc
     2581 gatacgccgg ggctggcgat ttcgccggcg tttgcgcgcg ccatcgaccg cgcgctgacg
     2641 gtgcacccgg acgagcgcat ccgcagcatc accgagttcc gcgaggcgct ggaattgccc
     2701 accttctggg ccgaaatgca gcgccagagc gtcgagttgg gcgcgctgcg cagccattcc
     2761 ggccacaccg gcttcggcac gctggacggg ctggaggacg aggtggcgcc gtcaaaagcc
     2821 gagcctgtgg acgcgccggt cgacgccgag gccgaggcgg cacgcgccga catcgaacgg
     2881 cgacgccgcg agcgcgcccg gcgccaggct gccggccagg gccgcgcacg ggacgaaggc
     2941 gcgcgcgctg gcttgcacca gggaacgggg aaaacgg
//
LOCUS       DAOMVO010000299         2967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9849_length_2967_cov_12.367102, whole genome shotgun sequence.
ACCESSION   DAOMVO010000299 DAOMVO010000000
VERSION     DAOMVO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9849_length_2967_cov_12.367102"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..731)
                     /locus_tag="PKV30_11795"
     CDS             complement(51..731)
                     /locus_tag="PKV30_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRL30928.1"
                     /translation="MNGSLIDDLLGQLQGAPAGQIAQQLGTDPNTAQDAIAAALPMIV
                     GALGRNAQQPGGATDLFSALQRDHGAGAGGMDLGSMLGGLLGGGNAGGLGGLGGLLGS
                     VVGGGAPASRQLDAGGILGHIFGGAQPRAESGLGQATGLNSGQAGQLLKILAPIVMAF
                     LAKQVSARNMDAGGLGSALGQERARVQNQGGLGGGLMNAVLDQDGDGDVDLSDLLRMG
                     GSLLGGRR"
     gene            869..1630
                     /locus_tag="PKV30_11800"
     CDS             869..1630
                     /locus_tag="PKV30_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL30929.1"
                     /translation="MRVDGKSIIVTGAGGGIGEGIARRLASEGAAVIVNDINAALGEK
                     VTADINASGSRARFVAGDVTKSADWQALVAAAQAFGGKLDVIVNNAGWTHRNRPMLEV
                     SEAEFDKVYAVNMKSIYWSAIHGVPALRQAGGGSFINIASTAGVRPRPGLTWYNGSKA
                     AVIVTSKSMAAELGPQNIRVNCINPVFNPDTGLSAEFAGGPVDEARRARFLATIPLGR
                     FSTALDVANAALYLASDEAAFISGVCIEVDGARCV"
     gene            1926..2936
                     /locus_tag="PKV30_11805"
     CDS             1926..2936
                     /locus_tag="PKV30_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30930.1"
                     /translation="MRPTAAVLARPVLPRRLRPRGALAGATLALAALVGAGAQAANAP
                     FPTVTTPVKLDTRVLASASWYEALNTIVYEAGTYHMWYVNPDLTGVRHATSADGINFT
                     TLGGDLTLPANWWTAYGATVQPQINYLRVSRDGDGNWILMIWHPNGANQGPFNYNTSL
                     WLLGSNIANTAPTLIGPLPISPGGNHVGPFGIVGDRLYLGQDTAQAFGRYLLMPSTTS
                     SPPTTWPAGMVDAANAYAGTGLCSFATCPGDPNSSYIHNYGRTLDQGGVLGTYYTLYN
                     YNTWTRRSKQLWYMESTDGGDTWGSAQPLFTDGNAVTVDGLPNAGNFSLPEVTALGRV
                     IK"
BASE COUNT          497 a         1100 c          942 g          428 t
ORIGIN      
        1 cgggcgcacg atagcgcact cgaccactgc gccgactccc tcgcctccgc tcagcgccgc
       61 ccgcccagca ggctgccgcc catgcgcagc aggtcggaca gatcgacgtc gccatcgccg
      121 tcctgatcga gcacggcgtt catcagcccg ccgcccaggc cgccctggtt ttgcacgcgg
      181 gcgcgctcct ggccgagcgc gctgcccagg ccgccggcgt ccatgttgcg cgcgctgacc
      241 tgcttggcca gaaacgccat cacgatgggc gccagaattt tcagcaactg accggcctgg
      301 cccgagttga ggcccgtggc ctgccccagc cccgattcgg cgcgcggctg cgcaccgccg
      361 aagatgtggc ccaggatgcc gcccgcgtcc agctggcggc tggccggcgc gccgccgccg
      421 accacgctac ccagcaagcc gccgagcccg cccaggccgc cggcgttgcc gccgccgagc
      481 agaccgccga gcatgctgcc caggtccata ccgcccgcgc cggcgccgtg gtcgcgctgc
      541 agcgcgctga acaggtcggt ggcgccaccc ggctgctggg cgttgcggcc gagcgcgccc
      601 acgatcatgg gcagcgcggc ggcaatggcg tcttgcgcgg tgttggggtc ggtgcccagc
      661 tgctgcgcaa tctggccggc gggcgcgcct tgcagttggc caagcagatc gtcgataaga
      721 gagccgttca tgggggtgtc cttgcctgac aggtggtgaa tgaagtgggt tgaacgcgca
      781 tggtagtggg atcgccgaca cggcgccggt gccacggcgg gcgcggacta aactgcgcca
      841 acgtcctaca cgaatcaaaa ggagcaccat gcgcgtcgac ggcaaatcca tcatcgtcac
      901 cggcgccggt ggcggcattg gcgagggcat cgcgcgccgg ctggcgtccg aaggcgccgc
      961 cgtcatcgtg aacgacatca acgccgcgct gggcgaaaag gttacggccg atatcaacgc
     1021 cagcggcagc cgggcgcgct ttgtggcggg cgacgtgacg aaaagcgccg actggcaagc
     1081 actggtcgcc gccgcacagg cctttggcgg caagttggac gtgatcgtca acaacgccgg
     1141 ctggactcac cgcaaccgcc ccatgctgga agtgagcgag gccgagttcg acaaggtcta
     1201 cgccgtcaac atgaagagca tctactggag cgctatccat ggcgtgccgg cgctgcggca
     1261 ggcgggcggc ggcagcttca taaacatcgc ctccaccgcc ggcgtgcgcc cgcgcccggg
     1321 cctcacctgg tacaacggca gcaaggcggc ggtgatcgtc accagcaagt cgatggcggc
     1381 cgagctggga ccgcagaaca tccgcgtgaa ctgcatcaac cccgtgttca accccgacac
     1441 cggcctgtcg gccgaattcg ccggcggccc ggtcgacgag gcgcgccgcg cccggtttct
     1501 ggccaccatc ccgctggggc gcttttccac cgcgctggac gtggccaacg ccgcgctcta
     1561 cctggccagc gacgaggccg ccttcatcag cggcgtctgc atcgaggtgg acggcgcgcg
     1621 ctgcgtctga cgaccgcccg gccccacgga ccgaccaccc tccgcctgaa cccaccgacc
     1681 acgtcgggca ggcgtgttcg cgcgcctgcc cgcctgttgg cgcgcccaaa cccgggcgca
     1741 gccggcgtgg agcgcgcctg cgcggcagac cttgcacgcg ctttggtcgc gacaaatcga
     1801 ccagtactcc accgatttcc tgcaatctgg cacggaccag tgctgcgcct gcgcgcccat
     1861 tgcttcttta ggtgtacacg cgtcattaca ctttgaagca ccaaaaccac gatgccttga
     1921 tcaccatgcg accgaccgcc gccgtgcttg cccgccccgt cctgccccgt cgcttgcgcc
     1981 cgcgcggggc gcttgccggc gctactttgg cactggccgc gctggtgggc gcgggcgcgc
     2041 aagcggccaa cgcgccgttc cccaccgtga cgacgcccgt caagctggac acccgggtgc
     2101 tggcatcggc cagctggtat gaggcgctca acaccatcgt ctacgaggcc gggacgtacc
     2161 acatgtggta tgtcaacccc gatctcacgg gcgttcgcca cgccacctcg gcagacggga
     2221 tcaacttcac caccctcggg ggcgacctca ccctgcccgc caactggtgg acggcctatg
     2281 gcgccacagt gcagccccag atcaactacc tgcgcgtctc gcgcgacggc gacggcaact
     2341 ggatcctgat gatctggcac cccaatggcg ccaaccaggg cccgttcaac tacaacacca
     2401 gcctgtggct gctcggcagc aacatcgcca acaccgcgcc aacgctgatc ggcccgctgc
     2461 cgatcagccc gggcggcaac cacgtcggcc cgttcggcat tgtcggcgac cgcctctatc
     2521 tcggccaaga caccgcgcag gcctttgggc ggtatctgct catgcccagc acgaccagct
     2581 cgccacccac gacctggcca gccgggatgg tggacgccgc caacgcctat gcgggcaccg
     2641 gcctgtgctc gttcgccacc tgcccaggcg accccaattc gtcctacatc cacaactacg
     2701 gccgcacgct cgaccagggc ggtgtgctgg gcacctacta cacgctttac aactacaaca
     2761 cctggacccg acgcagcaag cagctgtggt acatggaaag cacggacggc ggcgacacct
     2821 ggggcagcgc ccagccgctg ttcaccgacg gcaatgccgt caccgtggat ggcctgccca
     2881 acgccggcaa cttcagcctg cccgaggtca cggccctagg gcgtgtcatc aaatgagcca
     2941 aacgacagtg gcagccagat gtatgga
//
LOCUS       DAOMVO010000300         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9892_length_2959_cov_22.227273, whole genome shotgun sequence.
ACCESSION   DAOMVO010000300 DAOMVO010000000
VERSION     DAOMVO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9892_length_2959_cov_22.227273"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PKV30_11810"
     CDS             <1..915
                     /locus_tag="PKV30_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL30931.1"
                     /translation="QQIINGLVLGSMYALIALGYTMVYGIIQLINFAHGEVLMVGALT
                     AWSVIGMMQASMPGAPGWAILIMAMLIACVVAAALNFVIEKVAYRPLRNSPRLAPLIT
                     AIGMSILLQTLAMIIWKPNYKPFPTLLPSAPFNIGGAFITPTQIMILSVTAVSLAVLM
                     WLVHYTKIGRAMRATAENPRVAGLMGVKPDVVISATFIIGAILAAIAGVMYASNYGTA
                     QHTMGFLPGLKAFTAAVFGGIGNLGGAVLGGILLGLIEAIGSGYIGQLTGGLLGSHYT
                     DIFAFIVLIIILTLRPSGLLGERVADRA"
     gene            1074..2264
                     /locus_tag="PKV30_11815"
     CDS             1074..2264
                     /locus_tag="PKV30_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30932.1"
                     /translation="MTQKNKIIAFVLCGIALLVLPLILQHFGNAWVRIADIALLYVML
                     ALGLNIVVGYAGLLDLGYVAFFAVGAYLYGLLASPHLTSTFAAIGAAFPNGFHTSLWL
                     VIPLALGLATITGVLLGIPVLKLRGDYLAIVTLGFGEIIRIFLNNLDHPYNITNGPKG
                     INQIDSVKIFGLDLGRPLTIGGYEIASVTLYYYLFLALVIFTVIICQRLQDSRIGRAW
                     MAIREDEIAAKAMGINTRNMKLLAFGMGASFGGVSGSMFAAFQGFVSPESFSLMESVM
                     IVAMVVLGGIGHLPGVILGAVLLAALPEVLRYVAGPLQAMTDGRLDSAILRQLLIALA
                     MIIVMLLRPRGLWPSPEHGKGRVPGGANKITPTTAQQNPPGVDFPADAVPGNTERRQY
                     PINP"
     gene            2280..>2959
                     /locus_tag="PKV30_11820"
     CDS             2280..>2959
                     /locus_tag="PKV30_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30933.1"
                     /translation="MSTEDTVLKVEGVSKRFGGLQALSDVGITIRRGQVYGLIGPNGA
                     GKTTFFNVITGLYTPDSGSFELAGQPYKPQAVHQVAKAGIARTFQNIRLFAEMTALEN
                     VMVGRHVRTRSGLFGAIFRTGGFKAEEDAIAERAQQLLDYVGIGKYANFRSRTLSYGD
                     QRRLEIARALATDPQLIALDEPAAGMNATEKVALRELIDRIRKDNRTILLIEHDVKLV
                     MGLCDRVTV"
BASE COUNT          477 a         1003 c          929 g          550 t
ORIGIN      
        1 cagcagatca tcaacggtct ggtgctgggc agcatgtatg ccttgatcgc cctgggttac
       61 accatggtct acggcatcat tcaactgatc aactttgcgc acggcgaagt gctgatggtc
      121 ggggcgctca ccgcctggtc ggtcatcggc atgatgcagg ccagtatgcc gggcgcgccg
      181 ggctgggcca tcttgatcat ggccatgctg atcgcctgcg tggtcgccgc cgcgctcaac
      241 ttcgtgatcg agaaagtggc ctaccggccg ctgcgcaaca gcccgcgcct ggcgccgctg
      301 atcaccgcca tcggcatgtc gatcctgctg cagacgctgg ccatgatcat ctggaagccg
      361 aactacaagc ccttcccgac cctgctgccg agcgcgcctt tcaacatcgg cggcgccttc
      421 atcacgccga cgcagatcat gatcctgtcc gtcaccgcgg tgtcgctggc ggtgctgatg
      481 tggctggtgc attacacgaa gatcggccgc gcgatgcgcg ccacggccga gaacccgcgc
      541 gtggcgggcc tgatgggcgt caagcccgac gtggtgatct cggccacctt catcatcggc
      601 gccattctgg cggccatcgc cggcgtgatg tacgccagca actacggcac ggcgcaacac
      661 accatgggtt tcctgccggg gctgaaagcc ttcacggcgg cggtgtttgg cggcatcggc
      721 aacctgggcg gcgcggtgct gggtggcatc ctgctggggc tgatcgaagc catcggctcg
      781 ggctacatcg gccagctcac cggcggcctg ctgggcagcc actacaccga catcttcgcc
      841 ttcatcgtgc tgatcatcat cctcacgctg cgcccctctg gcctgctggg tgaacgcgtc
      901 gccgacagag catgaacaac accccccctg agtcgccttc ggcgccttcc cccctctgct
      961 gcgcgaaggg ggacaacgcc agtgcccggc acaggtccgt gcacggcgtt cgcttgaatg
     1021 gcctgctccg cggccttcgg tgcgccatgg cgcacgcata acggatcact ttcatgacgc
     1081 agaagaacaa gatcatcgcc ttcgtgctgt gcggcatcgc gctgctggtg ctgccgctca
     1141 tcctgcaaca cttcggcaac gcctgggtgc gcattgccga catcgcgttg ctgtacgtga
     1201 tgctggccct gggcctgaac atcgtggtcg gctacgcggg cctgctcgac ttgggctacg
     1261 tggccttctt tgccgtcggc gcctacctgt acgggttgct ggcctcgccg cacctgacca
     1321 gcacttttgc cgccatcggc gcggcgttcc ccaacgggtt tcacacctcg ctctggttgg
     1381 tgattccgct ggcactgggg ctggccacca tcaccggcgt gctgctgggc attccggtgc
     1441 tcaagctgcg cggcgactac ctggccatcg tcacgctggg cttcggcgag atcattcgca
     1501 tcttcctgaa caacctggac catccctaca acatcaccaa cggccccaag ggcatcaacc
     1561 agatcgactc ggtgaagatt ttcgggctgg atctggggcg gccgctgacc attggcgggt
     1621 acgagatcgc gtcggtcacg ctgtactact acctgttcct ggcgctggtg atcttcaccg
     1681 tcatcatctg ccagcgcttg caggattcgc gcatcggccg cgcctggatg gccatccgtg
     1741 aagacgagat cgccgccaag gccatgggca tcaacacccg caacatgaag ctgctggcct
     1801 ttggcatggg cgcctcgttc ggcggcgtgt cgggctcgat gtttgcggcc ttccagggct
     1861 tcgtttcgcc ggaatcgttc agcctgatgg agtccgtgat gatcgtcgcc atggtggtgc
     1921 tgggcggcat tggccacctg ccgggcgtga ttctgggcgc cgtgctgctg gcggcgctgc
     1981 ccgaggtgct gcgctacgtg gccgggccgc tgcaggccat gaccgatggc cggctcgatt
     2041 cggccatcct gcgtcagttg ctgattgcgc tggccatgat catcgtcatg ctgctgcgcc
     2101 cgcgcggcct gtggccatcg cccgagcacg gcaagggccg cgtgcctggg ggcgccaaca
     2161 agatcacccc gaccacggcg cagcaaaacc cgccaggcgt ggactttccc gccgacgccg
     2221 ttccgggcaa caccgagcgc cgtcagtatc cgatcaaccc ttgagtgcag gacagcagca
     2281 tgagcacaga agacaccgtc ctcaaggttg aaggcgtctc caagcgattc gggggcctgc
     2341 aggccctgtc ggacgtcggc atcaccatcc ggcgtggcca ggtctatggc ctgattggcc
     2401 ccaacggcgc cggcaagacg actttcttca acgtcatcac aggcctgtac acgcccgaca
     2461 gcggcagctt cgagctggcc gggcaaccct acaagccgca ggctgtgcac caggtcgcca
     2521 aggccggcat cgcgcgcacg ttccagaaca tcaggctgtt tgccgagatg accgcgctgg
     2581 agaacgtgat ggtcggccgc cacgtgcgca cccgctcggg gctgttcggc gccatcttcc
     2641 gcaccggcgg cttcaaggcc gaggaagacg cgatcgccga gcgcgcacag caattgctcg
     2701 actacgtggg cattggcaag tacgccaatt tccgctcgcg cacgctgtcc tacggcgacc
     2761 agcgccggct ggagattgcc cgtgcgctgg ccaccgaccc gcagctgatc gcgctggacg
     2821 aaccggccgc cggcatgaac gccaccgaga aggtcgccct gcgcgagctg atcgaccgca
     2881 tccgcaagga caaccgcacc atcctgctga tcgagcacga cgtgaagctg gtgatgggcc
     2941 tgtgcgaccg cgtgacggt
//
LOCUS       DAOMVO010000301         2950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9928_length_2950_cov_12.686701, whole genome shotgun sequence.
ACCESSION   DAOMVO010000301 DAOMVO010000000
VERSION     DAOMVO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9928_length_2950_cov_12.686701"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="dnaK"
                     /locus_tag="PKV30_11825"
     CDS             <1..1307
                     /gene="dnaK"
                     /locus_tag="PKV30_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRL30934.1"
                     /translation="VDGEKQFEVLSTNGDTFLGGEDFDQRIIDYVITEFKKEAGVDLK
                     NDVLALQRLKEAAEKAKIELSSAQATDINLPYITADASGPKHLNIKLTRAKFESLVEE
                     LIERTIAPCRTAIKDAGVSVGDISDVILVGGQTRMPKVQEKVKEFFGKEPRKDVNPDE
                     AVAVGAAIQGQVLSGDRKDVLLLDVTPLSLGIETLGGVMTKMITKNTTIPTKFAQTFS
                     TADDNQPAVTIKVYQGEREMASGNKLLGEFNLEGIAPAPRGMPQIEVTFDIDANGILH
                     VSAKDKATGKENKITIKANSGLSEAEIQQMVKDAELNSAEDKKKLELIQARNQGDAMV
                     HSVRKSLTEHGDKLEAGEKSAIEDAIKAVEEAIKGDDKADIESKTETLMTASQKLGEK
                     MYADAAAAAQAAGAAPGGTPGAGAAAADDNVVDAEVKEVKKD"
     gene            1419..2549
                     /gene="dnaJ"
                     /locus_tag="PKV30_11830"
     CDS             1419..2549
                     /gene="dnaJ"
                     /locus_tag="PKV30_11830"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRL30935.1"
                     /translation="MATKRDYYEVLGVPKNASDEEIKKAYRKLAMKYHPDRNQGGVAV
                     EAEVKFKEAKEAYEMLSDPQKRAGYDQYGHAGVDPNMRGGPEGFGGFAEAFGDIFGDI
                     FGQQRGGRGGQQMYRGADLSYAMEITLEEAAAGKDAQIRIPSWDECDTCHGSGAKPGT
                     SATTCGTCHGQGVVQMRQGFFSVQQTCPHCRGTGKLIKDPCGVCHGQGKVKNQKTLEV
                     KIPAGIDDGMRIRSAGNGEPGTNGGPPGDLFIEIRLKKHDIFERDGDDLHCVVPISFA
                     AAALGGEIEVPTLNGKAAIDIPDGTQAGKQFRLRGKGIKGVRASYPGDLYCHISLETP
                     VKLTDHQRKLLKEFDESLKKGGAKHSPSAESWTDRLKSFFGA"
     gene            2663..>2950
                     /locus_tag="PKV30_11835"
     CDS             2663..>2950
                     /locus_tag="PKV30_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30936.1"
                     /translation="MHLSSSGTAALPPAPHFPAAPQSRALQLLNRPISPFGAALLAAL
                     WIAGIGNLALWRSMADLPEVANARGLAFGIGFGVAIAAIHVALFSLLAWRWT"
BASE COUNT          626 a          952 c          952 g          420 t
ORIGIN      
        1 atgtcgatgg cgaaaagcag ttcgaggtgc tgtccaccaa cggcgacacc ttcctcggcg
       61 gcgaagactt cgaccagcgc atcatcgact acgtcatcac cgagttcaag aaagaagccg
      121 gcgttgacct gaagaacgac gtgctggccc ttcagcgcct gaaggaagcg gctgaaaaag
      181 ccaagatcga gctgtcgagc gcgcaagcga ccgacatcaa cctgccctac atcacggccg
      241 acgcctcggg ccccaagcac ctgaacatca agctcacgcg cgccaagttc gagagcctgg
      301 ttgaagagct gatcgagcga accatcgccc cctgccgcac cgccatcaag gatgcaggcg
      361 tgagcgtggg cgacatcagc gacgtgattc tggtcggcgg ccagacccgc atgcccaagg
      421 tgcaggagaa ggtcaaagag ttcttcggca aggagccgcg caaggacgtc aaccccgacg
      481 aagccgtggc cgtcggcgcc gccatccagg gccaggtgct ctcgggcgac cgcaaggacg
      541 tgctgctgct cgacgtgacc ccgctgtctc tgggcattga aaccctgggt ggtgtgatga
      601 cgaagatgat caccaagaac accaccatcc cgaccaagtt cgcgcagacc ttctccaccg
      661 ccgacgacaa ccagccggcc gtgaccatca aggtctacca gggcgagcgc gagatggcca
      721 gcggaaacaa gctgctgggc gagttcaacc tggaaggcat tgcgcccgcg ccgcgcggca
      781 tgccgcagat cgaggtcact ttcgacatcg acgccaacgg catcctgcac gtgtcggcca
      841 aggacaaggc gaccggcaag gagaacaaga tcaccatcaa ggccaactcg ggcctgtccg
      901 aggccgagat tcagcagatg gtgaaggacg ccgagctgaa ctccgccgag gacaagaaga
      961 agctcgaact gatccaggcc cgcaaccagg gcgacgccat ggtgcacagc gtgcgcaagt
     1021 cgctcaccga gcacggcgac aagctggaag ccggcgagaa atcggcgatc gaagacgcga
     1081 tcaaggccgt cgaagaagcc atcaagggcg acgacaaggc cgatatcgag tcgaagaccg
     1141 agacgctgat gaccgcgagc cagaagctcg gcgaaaagat gtacgccgat gccgccgccg
     1201 cggcgcaggc cgcaggtgct gcccccggtg gcacgccggg tgccggcgct gctgccgccg
     1261 acgacaacgt ggttgacgcc gaagtgaaag aagtcaagaa ggactgaagc gacggcgcgc
     1321 gcgggtaatc accgccccag cgctgccttt gccgccgcgc agaagattcc ccggagtcgg
     1381 ctgcgcggcg tttgtctgag ccgtttcgag aaatattgat ggccacaaag cgcgactatt
     1441 acgaagtcct gggcgttccc aagaacgcca gcgatgagga aatcaagaag gcttatcgca
     1501 agctggcgat gaagtaccac cccgaccgca accagggcgg cgtggccgtg gaggccgagg
     1561 tcaagttcaa ggaggccaaa gaggcctacg agatgctgtc cgacccgcaa aagcgcgccg
     1621 gctacgacca gtacggccac gcgggcgtcg accccaacat gcgcggcggg ccggaaggct
     1681 ttggcggttt tgccgaggcg tttggcgaca tctttggcga catcttcggc cagcagcgtg
     1741 gcgggcgcgg cggtcagcag atgtaccgcg gcgccgacct gagctacgcg atggaaatca
     1801 cgctggaaga agccgccgcg ggaaaggacg cgcaaatccg catccccagc tgggacgagt
     1861 gcgacacctg ccacggctcg ggcgccaagc ccggcaccag cgccaccacc tgcggcacct
     1921 gccacggcca gggtgtggtg cagatgcgcc agggcttttt cagcgtgcag cagacctgcc
     1981 cgcattgccg cggcaccggc aagctcatca aggacccgtg cggtgtgtgc cacggtcagg
     2041 gcaaggtcaa gaaccagaaa acgctggaag tgaagattcc cgccggcatt gacgacggca
     2101 tgcgcatccg ctcggccggc aacggcgagc cgggcaccaa cggcgggccg ccgggcgatc
     2161 tgttcatcga gattcgtttg aaaaagcacg acatcttcga gcgcgacggc gacgatttgc
     2221 attgcgtggt gccgatcagc tttgccgccg ctgcgctggg cggcgagatc gaggtgccga
     2281 cgctcaacgg caaggccgcc atcgacattc ccgacggcac gcaggccggc aagcagtttc
     2341 gcctgcgcgg caagggcatc aagggcgtgc gcgccagcta cccgggcgat ttgtactgcc
     2401 acatcagcct ggaaacgccc gtcaagctga ccgaccacca gcgcaagctg ctgaaagagt
     2461 tcgacgaatc gctgaaaaaa ggcggcgcca agcactcgcc cagcgccgag agctggaccg
     2521 atcggttgaa gagcttcttc ggcgcctgag tcgcgcttcg gcacacgcca agagaaccgc
     2581 cacggctgca aggcctggcg gttttttcat gcccacgcgc cgttaagacg cgcttcagac
     2641 ggcggcgcga gacttgcccg ccatgcacct ctcgtcatcg ggcacagcgg cgttgccgcc
     2701 agcgccccac tttcccgcag caccgcaatc gcgtgcgctg cagctgctga accgaccgat
     2761 ttcccccttt ggcgccgcgc tgctggcggc gctgtggatc gccggcatcg gcaaccttgc
     2821 cttgtggcgt tcgatggccg acctgcccga ggtggccaac gcacgcgggc tggcattcgg
     2881 catcggcttt ggcgtggcga ttgccgctat tcacgtggcg ctgttcagcc tgctggcctg
     2941 gcgctggacg
//
LOCUS       DAOMVO010000302         2936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_9982_length_2936_cov_11.282541, whole genome shotgun sequence.
ACCESSION   DAOMVO010000302 DAOMVO010000000
VERSION     DAOMVO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_2936_cov_11.282541"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1383
                     /locus_tag="PKV30_11840"
     CDS             154..1383
                     /locus_tag="PKV30_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRL30937.1"
                     /translation="MLFNGLGFERQVALRFLREGRMQTVLIIVGVAAGVAVIAYITAL
                     VNGLQGNTVAKTLGAQAHISLRAPDDVVTPAWPPTGGAARLTDTQPRAQRLRSVANWQ
                     ALVLQLEQRPEVRTVSPMVSGSGLALRGEASQAIALMGVDLDRYDRIAGLRGKTVSGQ
                     ARLEPGDAIVGTELAKDLGVRVGDRLSLNTGRVTDSVRVTALVDLGVKDLNRRTVIVP
                     LRAAQSLLGLAGGATVVDLQVRDVWQAEAVARELRRALPYKVESWQETNAQLVSALNA
                     QSISTTVIRLVVAVVVVLGIASVLVVSVVQKQREIGILRAMGATRGQVMRVFLVEGAV
                     VGALGSALGVLLALALIWVFTKLARGSDGLPLFVIHLPPETALQTALLAVVCGVLAAV
                     APARRAARLDPAQAIRL"
     gene            1380..>1612
                     /locus_tag="PKV30_11845"
     CDS             1380..>1612
                     /locus_tag="PKV30_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL30938.1"
                     /translation="MSVPSVSTNGDALIALHAIRKVYNAGLPSEVEVLHGIDWRVRRG
                     EFVALIGPSGSGKSTLLNIVGLLERPSGGEYRLL"
     assembly_gap    1613..1712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1713..1910
                     /locus_tag="PKV30_11850"
     CDS             <1713..1910
                     /locus_tag="PKV30_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL30939.1"
                     /translation="PTGNLDTAASDAVFALLRDIHDTRGTAFVIVTHDPRLAARCDRV
                     VELVDGRIARDEPTAPATERR"
     gene            1918..2850
                     /gene="dapF"
                     /locus_tag="PKV30_11855"
     CDS             1918..2850
                     /gene="dapF"
                     /locus_tag="PKV30_11855"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRL30940.1"
                     /translation="MRIRFTKMQGAGNDFVVLDETRARLNLTAAQYRFLGDRHFGVGA
                     DQILSVRPAPSTDVDFEYVIHNGADGGEVEQCGNGSRCFVRYVRDQGLTDNTSVRVKV
                     KNGVLTLSEQADGRVSVDMGAPVFELAKIPFNPRGLTPVPAGATPDRVWQLWPLDLKT
                     NLAPAPASQAQAAIVNVAVLSMGNPHAVQLVGDVDAAPVATHGPLIERHPAFPNRVNA
                     GFMQIVDRRHVRLRVYERGAGETLACGTGACAAVVAGIRLGLLDERVDVQTRGGALTI
                     EWAGGAPDHVFMTGPAVTVFEGSIDIPDDLSEPA"
BASE COUNT          409 a          937 c         1045 g          445 t
ORIGIN      
        1 cgggcgatgc ggtggtggtg gcgggcgatg cggcgccggg gaaggccgtg cgcgtgcgcg
       61 tgctgccgtg ggcgccgggc gcggccgcgg ggctgccagg cggtgccggc cccgatgacg
      121 cgtcgtcgct caccaacgcg atggggcgct agcgtgctgt tcaacgggct tggttttgag
      181 cgccaggtgg cgctgcgctt tctgcgcgaa ggccgcatgc agacggtgct catcatcgtc
      241 ggcgtggccg ccggcgtggc ggtgattgcc tacatcacgg cgctcgtcaa cggtctgcag
      301 ggcaatacag tggccaagac gctgggcgcg caggcgcaca tcagcctgcg cgcgccagac
      361 gacgtggtga cgcccgcgtg gccgcccacg ggcggcgccg cgcgcctgac cgacacgcag
      421 ccgcgcgccc agcgcctgcg ctcggtggcc aactggcagg cgctggtgct gcagcttgag
      481 caacggcccg aggtgcgcac cgtctcgccc atggtttcgg gcagcgggct ggcgttgcgc
      541 ggcgaggctt cgcaggccat tgcgttgatg ggcgtcgatc tggaccgcta cgaccgcatc
      601 gccggcctgc gcggcaagac ggtgagcggc caggcgcggc tggagccggg tgacgccatt
      661 gtcggcacag agctggccaa ggacttgggc gtgcgcgtag gcgaccgcct gagcctgaat
      721 accggccgcg tgaccgattc cgtgcgcgtg acggcgttgg tcgatctggg cgtgaaggac
      781 ttgaaccgcc gcaccgtcat cgtgccgctg cgcgcggcgc aaagcctgct cggcctggcc
      841 ggcggcgcca cagtggtcga cctgcaggtg cgcgacgtgt ggcaggccga ggcggtggcg
      901 cgcgagctgc ggcgcgcgct gccctacaag gtcgaaagct ggcaggagac caatgcgcag
      961 ctggtgtcgg cgctcaacgc gcagagcatc agcaccaccg tgatccggct ggtggtggcg
     1021 gtggtggtgg tgctgggcat cgccagcgtg ctggtggtgt cggtggtgca aaagcagcgc
     1081 gagatcggca tcctgcgcgc catgggcgcc acgcgcgggc aggtgatgcg cgtgtttctg
     1141 gtcgaaggcg cggtggtcgg cgcgctgggt tcggcgctcg gcgtgctgct ggcgctggcg
     1201 ctgatctggg tgtttacaaa attggcgcgc ggctccgacg ggctgccgct gttcgtcatc
     1261 cacctgccgc ccgaaacggc gttgcagacc gcgctgctgg ccgtggtgtg cggcgtgctg
     1321 gcggccgtcg ccccggctcg gcgcgcggcg cggctggacc cggcgcaggc gatccggctg
     1381 tgagcgtgcc atccgtgtca acgaatggcg acgcgctgat cgccctgcac gccatccgca
     1441 aggtctacaa cgctggcctg ccgagcgagg tggaggtgct gcacggcatc gactggcgcg
     1501 tgcggcgcgg cgagttcgtg gcgttgatcg gcccctcggg ctcgggcaag agcacgctgc
     1561 tcaacatcgt ggggctgctg gagcgcccga gcggcggcga ataccgactg ctnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccaccgg caacctcgac accgccgcgt
     1741 ccgacgccgt gttcgcgctg ctgcgcgaca tccacgacac gcgcggcacg gcgtttgtca
     1801 tcgtcacgca cgacccgcgc ctggcggcgc gctgcgaccg ggtggtggag ctggtggacg
     1861 gccgcatcgc gcgcgacgag cccaccgcgc ccgccaccga gcgccgataa tcgcgtcatg
     1921 cgcattcgct ttaccaagat gcagggtgcg ggcaacgatt tcgtggtgct cgacgaaacg
     1981 cgcgcgcggc tgaacctgac ggcggcgcaa tatcggtttc tgggtgaccg gcactttggg
     2041 gtcggggccg atcagatttt gagcgtgcgg cccgcgccct cgaccgatgt ggacttcgaa
     2101 tacgtgatcc acaacggtgc cgatggcggc gaggtcgagc aatgtggcaa cggctcgcgc
     2161 tgctttgtgc gctatgtgcg cgaccagggc ttgaccgaca acacgagcgt gcgcgtcaag
     2221 gtaaaaaacg gcgtgctgac gctctccgag caggccgacg gccgcgtcag cgtcgacatg
     2281 ggcgcgccgg tgttcgagct ggcgaagatt cccttcaatc cccggggcct gacccccgtg
     2341 ccagccggcg ccacgcccga ccgcgtgtgg cagctctggc cgcttgattt gaagacaaat
     2401 ctggctccag cgcccgccag tcaagcgcaa gcagctattg ttaatgtagc agtcttgtcc
     2461 atgggaaacc cacacgccgt gcagctggtg ggcgatgtgg atgccgcgcc ggtggccacg
     2521 cacggcccgc tcatcgagcg ccacccggcc ttcccgaacc gcgtcaacgc cggcttcatg
     2581 cagatcgtgg atcgccgcca cgtgcgcctg cgcgtgtacg agcgcggcgc gggcgaaacg
     2641 ctggcctgcg gcaccggcgc ctgcgcggcg gtggtggcgg gcatccggct cgggctgctg
     2701 gacgaacgcg tcgacgtgca aacgcgcggc ggcgcgctga cgattgaatg ggccggcggc
     2761 gcgcccgacc acgtcttcat gaccggccct gccgtcacgg tgtttgaagg cagcatcgac
     2821 attcccgacg acttatcaga accggcctga agcgccgcga acccccatgc aaacccagcc
     2881 gatcaccgaa gaagacatcg cccagttcct gatccacacg ccggatttct tcgagc
//
LOCUS       DAOMVO010000303         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10022_length_2927_cov_14.593663, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000303 DAOMVO010000000
VERSION     DAOMVO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10022_length_2927_cov_14.593663"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1248
                     /locus_tag="PKV30_11860"
     CDS             265..1248
                     /locus_tag="PKV30_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL30941.1"
                     /translation="MQHSQTHLRSRPIQAGHLRAFDAVARHLNFRLAAEEMALTQSAV
                     SRQIQALEEEVGVPLFLRHTRAVELTAAGMQLLQAVAQALPRIDTAVRQIRQSAGRRQ
                     VTVTTFASFASMWLIPRLEAFQREFADIDIRVDASDVAVDLEVTDVDVAVRYARAERM
                     PPGALRMFGESVTPMASPWLLKSGPPLKQPTDVLGHTLIEAADAYTPHLEWLTWRRWL
                     DTHGMARAQPKRWLSFNYAYQMTQAALSGQGIVLARPPLVAESLARGELVEVFPHTRL
                     HSPMAYWLLLAPRSAQRPEVKAFADWLSAQAVATREVTGEGEDSDLVNDLD"
     gene            complement(1330..1962)
                     /locus_tag="PKV30_11865"
     CDS             complement(1330..1962)
                     /locus_tag="PKV30_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRL30942.1"
                     /translation="MKPISRQNVEHTYGHYARFYDLVFGAVLQGGRRRMCEAVRAVAP
                     ATLLEVGVGTGLTLAQYPAETAVTGIDLSPEMLRHAEQRAAALPGRQIALHCMDAEAL
                     AFADGSFDCVTLPYVLSVTPDPARLTAELRRVCKPGGDIFVLNHFSGGNAVWALPEHL
                     VGGLAARIGFRSEFPFDEHMAHRGWTLLSRSKVNLLGLTTLVHLKNQPAA"
     gene            complement(2010..2477)
                     /locus_tag="PKV30_11870"
     CDS             complement(2010..2477)
                     /locus_tag="PKV30_11870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL30943.1"
                     /translation="MLIRTFALLAAGAALGLQAGCASQAPAVTHLNSGKVLPTGLPFS
                     EAVRVGNTVYLSGQLGNVPGPGPIKLAPGGMAGEAKQVMDNIQTALQAHGLSMADVVK
                     CTVMLADMSEWGAFNDVYKTYFSGNFPARSAFGSNGLAFNARVEVECIAAWRK"
     gene            complement(2692..>2927)
                     /locus_tag="PKV30_11875"
     CDS             complement(2692..>2927)
                     /locus_tag="PKV30_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRL30944.1"
                     /translation="YTRAKEQMFFETDTADSPWTVVKSDCKKRARLNAMRYVLHKLPY
                     NGKALDQVGKLDPLIVGRAHVVYERGERPQALM"
BASE COUNT          481 a         1000 c          987 g          459 t
ORIGIN      
        1 aaggtgaccc aggccgcgcc gcgctcgatg cacagctcgc ccgcgcggcg cgggcgcagc
       61 gccagcgcct ggccgggcgc caccgtgtgc aggggctggg cggccgccgc cagggccagc
      121 cgcgacaagg tgttctcggg tgcgttcatg atcggctccg tctgttgttt cgacatcaat
      181 cggtgacgcc atggtgcgct ggcgcgcatt gacagtccaa tgaaatcggg cgaggctatt
      241 gatgccgttc gcacatcaat cgacatgcag cattcgcaaa cccacctgcg ctcgcgcccg
      301 atccaggccg gccacctgcg cgccttcgac gcggtggcgc ggcacctcaa cttccggctg
      361 gcggccgagg agatggcgct gacacaatcg gccgtcagcc gccagatcca ggcgctggaa
      421 gaagaagtcg gcgtgccact atttttgcgc cacacgcgcg cggtagagct gacggcggcc
      481 ggcatgcagc tgctgcaggc ggtggcgcaa gcgctgccgc gcatcgacac ggcggtgcgg
      541 cagattcgcc agagcgccgg gcgccgccag gtcacggtga ccacctttgc ctcgtttgcg
      601 tcgatgtggc tgattccgcg gctggaggcg tttcagcgcg agtttgccga catcgacatc
      661 cgcgtcgacg ccagcgacgt cgcggtcgac ctggaggtga cggacgtcga cgtggcggtg
      721 cgctacgcgc gcgccgagcg catgccaccc ggcgcgctgc gcatgtttgg cgaatcggtc
      781 acgcccatgg ccagcccgtg gctgctgaaa agcgggccgc cgctgaagca gccgaccgac
      841 gtgctgggcc acacgctgat cgaggccgcc gacgcctaca ccccgcacct cgaatggctg
      901 acctggcgcc gctggctcga cacgcacggc atggcgcgcg cgcagcccaa gcgctggctc
      961 agcttcaact atgcgtacca gatgacacag gccgcgctgt cgggccaggg catcgtgctg
     1021 gcgcgcccgc cactggtggc tgagagcctg gcgcgcggcg agctggtgga agtttttccc
     1081 cacacgcggc tgcattcgcc catggcgtac tggctgctgc tggcgccgcg cagcgctcag
     1141 cggcccgagg tgaaggcgtt cgccgattgg ctgtcggcgc aggccgtggc cacgcgcgag
     1201 gtcacgggcg agggcgagga ttcagatctg gtgaacgatc tggattgatc gctgttgctt
     1261 ctgaattcat agctgcttgc gcttgtccat caagcgctgg aggcataaaa cacctaaaaa
     1321 agcaggtgct caagcggccg gctgattctt cagatgcacc agcgtggtca gtcccagcag
     1381 gttgaccttg ctgcgggaga gcagcgtcca cccacggtgc gccatgtgct cgtcaaacgg
     1441 gaactctgag cgaaagccga tgcgcgccgc cagcccgccc accagatgct cgggcagcgc
     1501 ccacaccgcg ttgccgccgc tgaaatggtt cagcacgaag atgtcgccgc ccggcttgca
     1561 cacgcgccgc agctcggcgg tgagccgcgc cgggtcgggc gtgaccgaca gcacgtaggg
     1621 cagggtgacg caatcgaagc tgccatcggc aaacgccagc gcctcggcat ccatgcagtg
     1681 cagcgcgatc tggcggccgg gcagggcggc ggcgcgctgc tcggcgtggc gcagcatctc
     1741 gggcgacagg tcgatgccgg tcacggccgt ttcggccggg tactgcgcca gcgtcagtcc
     1801 ggtgccgacg ccgacttcga gcagggtggc cggcgccacc gcgcgcaccg cctcgcacat
     1861 ccggcgccgg ccgccctgca ggacggcgcc aaacaccagg tcgtaaaacc gcgcgtagtg
     1921 gccgtaggtg tgctcgacgt tctggcggct gatgggcttc atgggcggcg cggattctat
     1981 cggccggcgc tttgggtcaa gtggacggct cacttgcgcc aggccgcgat gcactccacc
     2041 tcgacccgag cgttgaacgc cagcccgttg ctgccaaagg cgctgcgggc cgggaagttg
     2101 ccgctgaaat aggtcttgta gacgtcgttg aaagcgcccc attcgctcat gtcggccagc
     2161 atcaccgtgc acttgaccac atcggccatc gacaggccat gcgcctgcag cgcggtctgg
     2221 atgttgtcca tcacctgttt cgcctcaccc gccatgccgc ccggcgccag cttgatcggg
     2281 cccgggccgg gcacgttgcc gagctgcccc gacaggtaga ccgtgttgcc gacccgcacc
     2341 gcctcggaaa acggcagccc ggtcggcagc accttgcccg agttgaggtg cgtcaccgcc
     2401 ggcgcctgcg atgcgcaacc ggcctgaagc cccagcgccg cgccagcggc cagcaacgcg
     2461 aatgtgcgga tcaacatgga agtctcctca tatcgttgaa atgaaagcga cccggcaaac
     2521 agagcaccgg gctcgcaaag cgtaccgcag cacaagcccg cagggctgcg cagcaagccc
     2581 tttcaaaccg ccgcgaagca ggcgataccg gaacgccccg ctcggggttc ccgcgagcgc
     2641 gaagcgttgt ccccctgggg ggaaggcggc cgcaggccga ctcagggggg atcacatcaa
     2701 ggcctgcggc cgctcccccc gctcatacac cacatgcgcg cgcccgacga tcagcggatc
     2761 gagcttgccg acctgatcga gcgccttccc gttgtagggc agcttgtgca gcacatagcg
     2821 catggcgttc aggcgcgcgc gtttcttgca atcgcttttc accaccgtcc acgggctgtc
     2881 ggcggtgtcg gtctcgaaga acatctgctc cttggcgcgg gtgtagt
//
LOCUS       DAOMVO010000304         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10128_length_2903_cov_12.729284, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000304 DAOMVO010000000
VERSION     DAOMVO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10128_length_2903_cov_12.729284"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKV30_11880"
     CDS             complement(<1..641)
                     /locus_tag="PKV30_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRL30945.1"
                     /translation="MDLRPLALQALCEADLEQKLAQVAALKAQAAALSIVPESLLPEP
                     AALPGRPARPALIDPAKVPQRSVHTPEGRAALIHAICHIEFNAINLALDAIWRFADMP
                     EDFYRDWLRVAIEEAYHFKLLHEHLRMQLGHAYGDFPAHDGLWAMCEKTKDDITARMA
                     LVPRTLEARGLDATPLIQNKLRAVGTPDALAACDILDVILRDEIGHVAIGNRW"
     gene            690..1211
                     /locus_tag="PKV30_11885"
     CDS             690..1211
                     /locus_tag="PKV30_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRL30946.1"
                     /translation="MAVYEVDGMTPQVDATAWIADSAQVMGNVTIGPDASVWFGCVLR
                     GDTESMSIGEGSNIQDLTVMHADLGLPLTIGKHVTVGHKVMLHGCTIGDESLIGIGAV
                     VLNGARIGKSCLVGAGSLVTEGKEFPDGSMIMGTPAKVVRQLTPEQIEGLRQSAQHYI
                     DNARKFRATLKKL"
     gene            1362..2351
                     /locus_tag="PKV30_11890"
     CDS             1362..2351
                     /locus_tag="PKV30_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRL30947.1"
                     /translation="MSELHKFIFDGLPVRGQLVRLTDAWQEVLRRRAANTQTGAYPPP
                     VAEMLGEMTAAAVLMQSNIKFDGALIMQIFGDGPVKVAVAEVQPDMRLRATVSIVGAV
                     PPGARLPEMVNAHGQGRCAITLDPKNRQDGQQPYQGVVPLADEGGAPLMDLAAVVEHY
                     MRQSEQLDTTLVLAADEQVAAGLLIQRLPVQGQDNLSQSSVAREDDAGEAAEDDYQRI
                     AILAKSLKREELLGLDADTILRRLFWEENLMRFEPQAGASGPRFACSCSRERVAAMLK
                     GLGQQEADSIIAEQGQIEVSCDFCGQQERFDAVDVGRLFTPAAQQSPQPGSVQ"
     gene            2470..>2903
                     /locus_tag="PKV30_11895"
     CDS             2470..>2903
                     /locus_tag="PKV30_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRL30948.1"
                     /translation="MRFDVLTLFPELFAPFVEAGITRRAYASGQIVLKLWNPRDYAEG
                     NYRRVDDRPFGGGPGMVMLAEPLARCLAAVQADRADNDPGPVVLFSPIGQPLNHAAVE
                     HWSAGQGAILICGRYEGIDQRFIDAHVTHQISLGDFVLSGGE"
BASE COUNT          481 a          938 c         1048 g          436 t
ORIGIN      
        1 taccagcggt tgccgatggc gacgtggccg atctcgtcgc gcaggatgac gtcgaggatg
       61 tcgcaggcgg ccagcgcgtc gggcgtgccg acggcgcgca gcttgttctg gatgagcggc
      121 gtggcgtcga ggccgcgcgc ctccagcgtg cggggcacca gcgccatgcg ggcggtgatg
      181 tcatccttcg tcttttcgca catggcccac aggccgtcgt gcgcggggaa gtcgccatac
      241 gcgtggccca gctgcatgcg caggtgctcg tgcagcagct tgaagtgata cgcctcttcg
      301 atggccacgc gcagccagtc gcggtagaaa tcctcgggca tgtcggcaaa gcgccagatc
      361 gcgtcgagcg ccaggttgat ggcgttgaac tcgatgtggc agatggcgtg gatgagcgcc
      421 gcgcggcctt cgggcgtgtg cacgctgcgc tggggcacct tggcggggtc gatcagtgcg
      481 ggccgggcgg ggcggccggg cagggcggca ggctctggca gcagcgactc gggcactatc
      541 gaaagcgcag ctgcctgcgc tttgagggcg gcgacttggg ccagtttctg ttcaagatcg
      601 gcttcgcaaa gggcttgaag ggcaaggggg cgcaggtcca ttcgtagaat tctatgaacc
      661 ccaacccgaa caacccagga gacgaagcga tggcggtgta tgaagtggac ggcatgacgc
      721 cgcaggtgga cgcgacggcg tggattgccg attcggccca ggtgatgggc aacgtgacga
      781 tcggccccga tgccagcgtg tggttcggct gcgtgctgcg cggcgacacc gagagcatgt
      841 cgatcggcga gggcagcaac atccaggacc tgacggtgat gcacgccgac ctcggcctgc
      901 cgctgaccat cggcaagcac gtgacggtgg ggcacaaggt gatgctgcac ggttgcacca
      961 tcggcgacga gtcgctgatc ggcatcggcg cggtggtgct gaacggcgcc aggatcggca
     1021 agagctgcct ggtgggcgcg ggctcgctgg tgacggaggg caaggagttc ccggacggca
     1081 gcatgatcat gggcacaccg gccaaggtgg tgcgccagct cacgcccgag cagatcgagg
     1141 gcctgcgcca gagcgcgcag cactacatcg acaacgcgcg caagttccgc gcgacgctga
     1201 agaagctgta aatcggctgg tagtcccgtt ttttggtttg ggagcgggct tgcccgcgat
     1261 ggggcgccag cccaacgcga ggcgctgtca tcgcgggcaa gcccgctccc acagcttgca
     1321 cgcccggttg acaaacccgg gcctgcccca aaataccccg gatgtccgaa ctgcacaaat
     1381 tcatcttcga cggcctgccg gtgcgcggcc aactggtgcg cctgaccgac gcctggcaag
     1441 aggtgctgcg ccgccgcgcc gccaacaccc agaccggcgc ctacccgccg ccggtggccg
     1501 agatgctggg cgagatgacc gccgccgcgg tgctgatgca gagcaacatc aagttcgacg
     1561 gcgcgctgat catgcagatc ttcggcgacg gcccggtgaa ggtggccgtg gccgaggtgc
     1621 agcccgacat gcggctgcgt gccacggtct ccatcgtcgg cgccgtgccg cccggcgcgc
     1681 gcctaccgga gatggtgaac gcgcacggcc agggccgctg cgccatcacg ctcgacccca
     1741 agaaccgcca ggatggccag cagccctacc agggcgtggt gccgctggcc gacgaaggcg
     1801 gcgcgccgct gatggacctg gccgccgtgg tggagcacta catgcgccag tccgagcagc
     1861 tcgacaccac gctggtgctg gccgccgacg agcaggtggc cgccggcctg ttgatccagc
     1921 ggctgccggt gcaggggcag gacaacctgt cgcaaagcag cgtggcgcgc gaagacgatg
     1981 ccggcgaagc ggccgaggac gactaccagc gcatcgccat cctggccaag agcctgaagc
     2041 gcgaggaact gctgggcctg gacgccgaca ccatcttgcg ccgcctgttc tgggaagaga
     2101 acctgatgcg cttcgagccg caggccggcg ccagcgggcc gcgctttgcc tgctcgtgca
     2161 gccgcgagcg cgtggccgcc atgctgaagg ggctgggcca gcaagaggcc gactcgatca
     2221 ttgccgagca gggccagatc gaggtgagct gcgacttctg cggccagcag gagcgcttcg
     2281 acgcggtgga cgtggggcgc ctgttcacgc ccgccgcgca gcaatcgcct cagccgggat
     2341 cggttcaata gagggggagt atatggtctc agcgcttgct gatcgggcgc tggcggccat
     2401 atgtaggggg agtatgtgat aaacagccct gaatccaaga gcacgccagc ggcgcccgcg
     2461 cgcgcgccgc tgcgcttcga cgtgctgacg ctgttccccg agctgttcgc acccttcgtc
     2521 gaggccggca tcacccgccg cgcctatgcc agcggccaga tcgtgctgaa gctgtggaac
     2581 ccgcgcgatt acgcggaagg caactaccgc cgcgtcgacg accgcccctt cggcggcggc
     2641 cccggcatgg tgatgctggc cgagccgctg gcgcgttgcc tggcggcggt gcaggccgac
     2701 cgcgctgaca acgacccggg gccggtggtg ctgttctcgc ccatcggcca gccgctgaac
     2761 cacgccgccg tcgaacactg gtcggcgggg cagggcgcca tcctgatctg cggccgctat
     2821 gagggcatcg accagcgctt catcgatgcc cacgtcacgc accagatcag cctgggcgat
     2881 ttcgtgctct cgggcggcga gat
//
LOCUS       DAOMVO010000305         2902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10131_length_2902_cov_13.701440, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000305 DAOMVO010000000
VERSION     DAOMVO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10131_length_2902_cov_13.701440"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV30_11900"
     CDS             complement(<1..242)
                     /locus_tag="PKV30_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRL30949.1"
                     /translation="MTAAPVQGEDGLVLHQHDARGVHRLTLNSPKSFNVLGEAVLGEL
                     QRQFDAIGADPTARVLVIAAEGKAFCAGHDLKQMKA"
     gene            complement(311..1264)
                     /locus_tag="PKV30_11905"
     CDS             complement(311..1264)
                     /locus_tag="PKV30_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRL30950.1"
                     /translation="MGKTGYFTHPDCRKHDMGAGHPECPERLDAIEDRLLISGVGDAL
                     ERREATPAAMGELLLGHGRRHVAALRGLNDILVEEMLAGGPDHYALDPDTSMNQHTYR
                     AALLSAGAAIDAVDAVMTGELENAFCCTRPPGHHATRNQAMGFCFFSNVAVAAKYACE
                     RHGVKRVAIIDFDVHHGNGTEDIVAGDERILMCSFFQHPFYPEGGSLKNDANLVNCPV
                     PAYTKGMDVRELVEMMWMPRLEEHQPELIFISAGFDAHREDDMGQMGLTESDYAWMTD
                     RIKSVAQRFAKGRIVSCLEGGYNLSALARSVEAHVRVLADV"
     gene            1317..2237
                     /locus_tag="PKV30_11910"
     CDS             1317..2237
                     /locus_tag="PKV30_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL30951.1"
                     /translation="MNVTQKIKAVVDQLNTVMVGKSVQVQDGVCCLLAGGHLLIEDVP
                     GVGKTTLAHALARTFGLQFSRVQFTADLMPSDLSGVSVYERGQERFVFHPGPVFAQVL
                     LADEINRASPKTQSALLEAMEEKQVTVEGETRALPWPFFVIATQNPHDQLGTYALPES
                     QLDRFLMRISLGYPERAAERELLSGNGRRDMLEQLPAVLNAEDLAALQQAVGRVHVAE
                     PLLDYVQDLVAATRNGRWFLQGLSPRAGIALMRAARAQALISGRDYVAPDDVQAILPQ
                     CVAHRMIPVSDAGRGAVEQVRAMIDAVPLP"
     gene            2395..>2902
                     /locus_tag="PKV30_11915"
     CDS             2395..>2902
                     /locus_tag="PKV30_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRL30952.1"
                     /translation="MRARANARLRRWWHDRLPLSDTLTLTQRNVYILPTRAGWMLAAT
                     LIVLLIASINYQLNLGYLLTFLLAGSAAAGMVVCHNTLRGLTLHLLLPQPLFAGSLAV
                     LDVKLVNPRRTPRHAIGVALLDTDNWSWADVPAQSESAVQVSFKPARRGLHRVPTLTA
                     ETRFPLGTF"
BASE COUNT          459 a          991 c          935 g          517 t
ORIGIN      
        1 ttggccttca tctgcttgag gtcgtggccg gcgcaaaagg ccttgccctc ggcggcgatg
       61 accagcacac gcgccgtcgg gtcggcgcca atggcatcga actggcgctg caattcgccc
      121 agcaccgctt cgcccagcac gttgaaggac ttcgggctgt tcagcgtcag ccggtgcacg
      181 ccacgcgcgt cgtgctggtg cagcaccagg ccgtcctcgc cttgaacggg ggctgctgtc
      241 atgatatcga aactatgttt ttaatagctg ctcgcgcttg tccatcaagc gctggcggcc
      301 attttaatgc tcaaacgtcg gccagcacac gcacgtgcgc ctcgacgctg cgcgccagcg
      361 ccgacaggtt gtagccgcct tccaggcagc tcacgatgcg acccttggca aagcgctggg
      421 ccaccgactt gatgcgatcg gtcatccacg cgtagtcgct ctcggtcagg cccatctggc
      481 ccatgtcgtc ctcgcggtgc gcgtcaaagc cggcgctgat gaagatcagc tcgggctggt
      541 gttcttccag gcgcggcatc cacatcatct cgaccagctc gcgcacgtcc atgcccttgg
      601 tgtacgcggg caccgggcaa ttgaccaggt tggcgtcgtt cttcaacgat ccgccctcgg
      661 ggtagaacgg gtgctggaaa aagctgcaca tcaggatgcg ctcgtcaccg gccacgatgt
      721 cttcggtgcc gttgccatgg tgcacgtcga agtcgatgat ggccacgcgc ttgacgccgt
      781 ggcgttcgca cgcgtacttg gcggccacag cgacgttgct gaaaaagcaa aagcccatgg
      841 cctggttgcg cgtggcgtgg tggccgggcg gacgggtgca gcaaaaggcg ttttccagct
      901 cgccggtcat caccgcgtcc accgcgtcga tggcggcgcc ggccgacagc agcgcggcgc
      961 ggtaagtgtg ctggttcatc gacgtgtcgg ggtcgagcgc atagtggtcg ggcccgccgg
     1021 ccagcatctc ttcgaccagg atgtcgttca acccgcgcag cgcggccacg tgacggcggc
     1081 cgtggcccag cagcaattcg cccatggcgg cgggcgtggc ctcgcgccgc tccagcgcat
     1141 cgccgacgcc tgaaatcagc aaacgatcct ctatcgcatc cagccgctcg gggcattcgg
     1201 gatgacccgc gcccatgtcg tgcttgcggc aatcggggtg tgtgaaatac ccggttttac
     1261 ccacgcttgt ctccttgtcc cacgatacgg cttcgaatat cagttacctt ccttgcatga
     1321 acgtgactca gaagatcaag gccgtcgtcg atcagcttaa cacggtgatg gtgggcaaat
     1381 cggtgcaggt gcaagacggc gtgtgctgcc tgctggccgg cggccacctg ttgatcgagg
     1441 acgtgccggg cgtgggcaag accacgctgg cgcacgcgct ggcgcgcacc tttggtctgc
     1501 agttttcgcg cgtgcagttc accgccgacc tgatgcccag cgatctttcg ggcgtgtccg
     1561 tgtacgagcg cgggcaggag cgcttcgtgt tccaccccgg cccggtgttt gcgcaagtgc
     1621 tgctggccga cgagatcaac cgcgccagcc ccaagacgca aagcgcgctg ctcgaagcga
     1681 tggaagaaaa gcaggtgacc gtcgaaggcg aaacgcgcgc cctgccctgg ccgtttttcg
     1741 tcatcgccac gcagaacccg cacgaccagt tgggcaccta cgccctgcct gagtcacagc
     1801 tcgaccgctt tctgatgcgc atcagcctcg gctaccccga gcgcgccgcc gagcgcgaac
     1861 tgctgtcggg caacggccgg cgcgacatgc tcgagcaact gcccgccgtg ctgaacgccg
     1921 aggacctggc cgcgctgcag caggccgtgg gccgcgtgca cgtggccgag ccgctgctcg
     1981 actacgtgca ggacctggtc gccgccacgc gcaacggccg ctggtttttg caaggcctgt
     2041 cgccgcgcgc cggcattgcg ctgatgcggg cggcgcgcgc gcaggcgctg atcagcgggc
     2101 gcgactacgt ggcgccagac gacgtgcaag ccatcctgcc gcaatgcgtg gcacaccgca
     2161 tgattcccgt gagcgatgcc gggcgcggcg cggtcgagca ggtgcgggcg atgatcgatg
     2221 cggtgccgct gccttgatcg tgctcgctcg gaaactacac ttttgatagc tgctcgcgca
     2281 cgtccaacaa gcgccagcgg ctgatttttc ttggaacttt tcgctgcgcg caacgatcct
     2341 gcaggcatgg cgtccaccac cgcttccacc gcgcccaccc cgccctggtg ggcgatgcgc
     2401 gcgcgcgcca acgcgcggct gcgccgctgg tggcacgacc gcctgccgct gtcggacacg
     2461 ctcacgctca cgcagcgcaa cgtctacatc ctgcccacgc gcgccggctg gatgctggcg
     2521 gccacgctca tcgtgctgct catcgcctca atcaactacc agctcaacct gggctatttg
     2581 ctgaccttct tgctggccgg cagcgcggcg gccggcatgg tggtgtgcca caacacgctg
     2641 cgcgggctga cgctgcacct gctgctgccg caaccgctgt ttgccggcag cctggcggtg
     2701 ctggacgtga agctggtcaa cccgcgccgc acgccgcgtc atgccatcgg cgtggccctg
     2761 ctcgacaccg acaactggtc gtgggccgat gtgccggcgc agagcgaaag cgcggtgcaa
     2821 gtcagcttca aacccgcgcg gcgcgggctg caccgcgtgc ccacgctgac ggccgaaacg
     2881 cgctttccgc tcggcacctt cc
//
LOCUS       DAOMVO010000306         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10136_length_2901_cov_10.861911, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000306 DAOMVO010000000
VERSION     DAOMVO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_2901_cov_10.861911"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKV30_11920"
     CDS             <1..638
                     /locus_tag="PKV30_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL30953.1"
                     /translation="DALKHFWNKWGNLITWVLIAVLGAYAAWNGWQYWQRRQAAMAAV
                     LYDEIDRAAKAGDTTRVERALVDMKDKYGGTTYAAYAGLQGAKLLADKGKLTEAKAAL
                     AAVADKAPDDGLRAVARLRLASVQTGEKAYDEALKTLSASFPAQYTALAADRRGDVLL
                     LQGKRSEAATEFGKAYQGLAAESGDYRRLVGMKLNALGIDPDAGAKAGATS"
     gene            650..1777
                     /gene="bamB"
                     /locus_tag="PKV30_11925"
     CDS             650..1777
                     /gene="bamB"
                     /locus_tag="PKV30_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HRL30954.1"
                     /translation="MTRHRWVLSGAGVLAAAVLAGCAGGSARPKPAELPPNVALVGVR
                     QAWTARLPAVAFPLQVNVQGQQVALAATDGTVVTLDGASGREIGRASAGAQLSAGVGS
                     DGRTLAVVTRDNQVVTLEGGRELWRRKLATQSYTAPLVAGGRVFVLGADRSLAAFDGR
                     NGAPLWRLERPAEPLVLRQAGMLQAMGNTLVAGMAGRMVGVDPDNGTVRWEAPLASAR
                     GTNDVERLIDLVGGVSRQNNTLCARAFQAVVGCVDTAAGNVQWTKAASGSTGIAGDGQ
                     RLFGTESDGRVLAWRADTGDRAWASEYLQWRQLTGPLVLGRSVVVGDSTGLVHLLSRE
                     DGRPLNRLTTDGSGIAATPVTVGNTLVVVTRNGGVYGFVPE"
     gene            2016..>2901
                     /gene="der"
                     /locus_tag="PKV30_11930"
     CDS             2016..>2901
                     /gene="der"
                     /locus_tag="PKV30_11930"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRL30955.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTKSRDAIVADFAGLTRDRHYGQ
                     GRLGNREYIVIDTGGFEPTAESGIFKEMAKQTRQAVAEADVVIFVVDARDGLSAQDHD
                     IANYLRRLGKPTLLVANKAEGMRAGTQLAEFYELGLGEVIPVSAAHGEGVRSMLEAAL
                     DTLNLPEPEDEPEPDPHAPIRLAVAGRPNVGKSTLINTWLGEERLVAFDLPGTTRDAI
                     SVEFERNGQKFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIESANVVLLLIDAEQGVT
                     DQDAHIAGFILDAGRAVVL"
BASE COUNT          450 a          984 c         1057 g          410 t
ORIGIN      
        1 tcgacgcgct caaacacttc tggaacaagt ggggcaacct gatcacctgg gtgctgatcg
       61 ccgtgctggg tgcctacgcc gcctggaacg gctggcaata ctggcagcgg cgccaggcgg
      121 ccatggcggc cgtgctgtac gacgaaatcg accgcgccgc caaggccggc gacaccaccc
      181 gcgtcgaacg cgcgctggtc gacatgaagg acaagtatgg cggcaccacc tacgccgcct
      241 acgccggcct gcaaggcgcc aagctgctgg ccgacaaggg caagctcacc gaggcaaaag
      301 ctgcgctggc cgccgtggcc gacaaggcgc ccgacgacgg gctgcgcgcc gtcgcgcgcc
      361 tgcgcctggc atcggtgcag acgggcgaaa aagcgtatga cgaggcgctg aagacgctca
      421 gcgccagctt tccagcccaa tacacggcgc tggccgccga ccgccggggc gacgtgctgc
      481 tgctgcaagg caagcgctcc gaggccgcca ccgaattcgg caaggcctat caaggcctgg
      541 cggccgagag cggcgattac cgccgcctgg tcggcatgaa gctcaatgcg ctgggcatcg
      601 accccgatgc cggcgccaag gcgggggcga cgtcgtgagc gcgcgccgca tgacgcgtca
      661 ccgctgggtg ctgtccggcg caggcgtgct ggccgccgcc gtgctggccg gctgcgccgg
      721 cggctcggcg cgccccaagc cggccgagct gccgcccaac gtcgccctgg tcggcgtgcg
      781 gcaggcgtgg acagcccggt tgccggcggt ggcctttccg ctgcaggtca atgtgcaggg
      841 gcagcaggtg gcgctggcgg ccaccgatgg caccgtggtc acgctggatg gcgccagcgg
      901 tcgtgaaatc ggccgtgcca gcgccggcgc gcagttgtcg gccggcgtcg gcagcgacgg
      961 ccgcacgctg gccgtggtca cgcgcgacaa ccaggtggtg acgctggagg gcgggcgcga
     1021 gttgtggcgc cgcaagctcg ccacgcaaag ctacaccgcg ccgctggtgg ccggcggccg
     1081 cgtcttcgtg ctcggcgccg accgttcgct ggccgccttc gacggccgca atggcgcgcc
     1141 gctgtggcgg ctggagcgcc ccgccgagcc gctggtgctg cgccaggccg gcatgctgca
     1201 ggccatgggc aacacgctgg tggcgggcat ggccggccgc atggtcggcg tcgacccgga
     1261 caacggcacc gtgcgctggg aagcgccgct ggcctcggcg cgcggcacca acgatgtcga
     1321 gcgcctgatc gatctggtcg gcggcgtgag ccggcagaac aacaccctgt gcgcgcgcgc
     1381 cttccaggcc gtggtcggct gtgtcgatac ggcggccggc aacgtgcaat ggactaaagc
     1441 cgccagcggc tcgaccggca tcgcgggcga cggccagcgc ctgttcggca ccgaatccga
     1501 cggccgcgtg ctggcctggc gcgccgacac cggcgaccgc gcctgggcgt ctgaatacct
     1561 gcagtggcgt cagttgaccg gcccgctggt gctgggccgc tcggtcgtcg tgggcgacag
     1621 caccggcctg gtgcacctgc tgtcgcgcga agacggccgc ccgctgaacc gcctgaccac
     1681 cgatggctcc ggcattgcgg ccacgccggt gacagtgggc aacacgctgg tggtggtgac
     1741 gcgcaatggc ggggtgtacg ggttcgtgcc cgaatgaatc ggctccccct gaggcgctga
     1801 cgcgccctcc cccagagggg gacaacgctg gtggccgggg ggaccccggt cacggcattc
     1861 cacagtggcc tgctccgcgg ccttctggtt cggcctgctg cgcggccctg ggagggcggt
     1921 atttgggtga aaatggcctc tagcgcttga tggacaagcg cgagcagcta tcgttttgat
     1981 acttcctgcc gctggcggga gtgaaaggtg gggcgatgaa acccgtgatc gcgctggtgg
     2041 ggcggcccaa tgtgggcaaa tccacgctgt tcaaccggct gacgaaaagc cgcgatgcca
     2101 tcgtggccga ctttgcgggg ctgacgcgcg accggcacta tggtcagggg cggctgggca
     2161 accgcgaata catcgtcatc gacaccggcg gcttcgagcc gacggccgaa tcgggcatct
     2221 tcaaggaaat ggccaagcag acgcgccagg ccgtggccga ggccgatgtg gtgatcttcg
     2281 tggtcgatgc gcgcgacggc ctgtcggcgc aggaccacga catcgccaat tacctgcgcc
     2341 gcctgggcaa gccgacgctg ctggtggcca acaaggccga gggcatgcgc gccggcacgc
     2401 agctggccga gttctatgaa ttgggtctgg gcgaggtgat tcccgtctcg gccgcgcacg
     2461 gcgagggcgt gcgcagcatg ctggaagccg cgctcgacac cctgaatctg cccgagccgg
     2521 aggacgagcc cgaacccgac ccccacgcgc ccatccgcct ggcggtggcg gggcgcccca
     2581 acgtcggcaa atccaccctc atcaacacct ggctgggcga agagcgcctg gtggcctttg
     2641 atctgcccgg caccacgcgc gatgccatct cggtcgagtt tgagcgcaat ggccagaagt
     2701 tcgagctgat cgacaccgcc ggcctgcgcc gcaagggcaa ggtgttcgag gcgatcgaga
     2761 agttctcggt cgtcaagacg ctgcaggcca tcgaatcggc caacgtggtg ctgctgctga
     2821 tcgacgccga gcagggcgtg accgaccagg acgcgcacat cgccggcttc atcctcgacg
     2881 ccggccgcgc cgtggtgctg g
//
LOCUS       DAOMVO010000307         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10197_length_2888_cov_13.838334, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000307 DAOMVO010000000
VERSION     DAOMVO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_2888_cov_13.838334"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKV30_11935"
     CDS             <1..294
                     /locus_tag="PKV30_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase"
                     /protein_id="HRL30956.1"
                     /translation="GAAPREIDLFHPTAVEDTAALNKVRADAQAAGVRLHVLVDARDG
                     HLSGERLRQTVPGWREASVWFCGPAGFGKALRADLLAQGLPADAFHQELFEMR"
     gene            431..1591
                     /locus_tag="PKV30_11940"
     CDS             431..1591
                     /locus_tag="PKV30_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRL30957.1"
                     /translation="MKRLWLVFAQTVTVLLAAYFVVATLKPQWLNRGVAVPGSTGTIS
                     ILEAPAAPIGTAAAPGSLAGAVRQAAPAVVSINTSTSARGPQSNDPWFRFFFGDRGDL
                     QQQQTGLGSGVIMSPEGYVLTNNHVVEGADAIEVVLPDARRTTAKVIGTDPESDLAVL
                     KITLDKLPAITLGNSDALQVGDQVLAIGNPFGVGQTVTSGIVSALGRNQLGINTFENF
                     IQTDAAINPGNSGGALTDINGNLMGINTAIYSRSGGSMGIGFAIPVSTAKQVMESIVK
                     NGQVVRGWIGVEPNELTPELAQTFGIQLAQGVIVTGVLNSGPAAEAGIRPGDVITQVA
                     GQPVRTVSELLTRIAGLTPGQATPFALQRQGQAVEVSVTPAQRPRPQRVMQR"
     gene            complement(1695..2498)
                     /gene="tatC"
                     /locus_tag="PKV30_11945"
     CDS             complement(1695..2498)
                     /gene="tatC"
                     /locus_tag="PKV30_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRL30958.1"
                     /translation="MTEPNPKTDPEDELAGTEQPFVAHLMELRDRLIKALVAVGVVAG
                     VLALYPGPGALYDLLAAPLIAHLPAGATMIATSVISPFMVPLKILLMAAFLVALPVVL
                     YQVWAFVAPGLYSHEKKLVLPLVVSSTILFFLGVAFCYFFVFGKVFTFIQSFAPKSIT
                     AAPDIEAYLTFVLTMFMAFGLAFEVPIAVVVLARLGVVTVEQLKQFRGYFIVLAFVIA
                     AIVTPPDVVSQLALAIPMCLLYEVGIWAAQLFIKHTNAPEDDAAEAAKS"
     gene            complement(2635..>2888)
                     /gene="tatB"
                     /locus_tag="PKV30_11950"
     CDS             complement(2635..>2888)
                     /gene="tatB"
                     /locus_tag="PKV30_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HRL30959.1"
                     /translation="GLHSTKADFEKDWSSVTAGLDGSGTASDSAPAWQPPAPEYRRPD
                     KNWRLKRGATPQWYKARHGVRRQVQSGAARVARYRPKNK"
BASE COUNT          496 a          984 c          974 g          434 t
ORIGIN      
        1 ggcgccgcgc cgcgcgagat cgacctgttc caccccaccg ccgtcgaaga caccgccgcg
       61 ctgaacaagg tgcgcgccga tgcccaggcc gccggcgtgc gcctgcacgt gctggtggac
      121 gcgcgcgacg ggcacctgtc gggcgagcgc ctgcgccaga cggtgccagg ctggcgcgaa
      181 gccagtgtgt ggttctgcgg ccccgccggc tttggcaagg cgctgcgcgc cgacctgctg
      241 gcgcagggcc tgccggccga tgcgttccat caggagctgt tcgagatgcg ttgacgccgc
      301 tcgccaccac cacccgcaca cgcgggtggc gccttgaaaa accacctcgc gcctacaatt
      361 tgcccccttg gcgccggccg cgccgcccct gtcgggcgcg ggctttggac gccgaacgga
      421 aaccgcccgc atgaaacgcc tctggctcgt ctttgcccaa accgtcaccg tgctgctggc
      481 cgcgtacttc gtcgtcgcca cgctcaagcc gcagtggctg aaccgtggcg tggcggtgcc
      541 cggcagcacc ggcaccatct cgattctgga ggcgcccgcc gcgcccattg gcacggcagc
      601 ggcgcccggc agcctggctg gcgcggtgcg ccaggcggcg ccggcggtgg tcagcatcaa
      661 caccagcacc agcgcgcgcg gcccgcaaag caacgacccg tggttccgct tcttctttgg
      721 cgaccgcggc gacctgcagc aacagcaaac cggcctcggc tcgggcgtga tcatgagccc
      781 cgagggctac gtgctgacca acaaccacgt ggtcgaaggc gccgacgcca tcgaggtggt
      841 gctgcccgac gcgcgccgta ccacggccaa ggtgatcggc acggaccccg agagcgatct
      901 ggcggtgctg aagatcacgc tggacaagct gccggccata acgctcggca attccgacgc
      961 gctgcaggtg ggcgaccagg tgctggccat cggcaacccg tttggcgtcg gccagacggt
     1021 gaccagcggc atcgtctcgg cgctggggcg caaccagttg ggcatcaaca cgttcgaaaa
     1081 cttcatccag accgatgccg ccatcaaccc cggcaactcg ggcggcgcgc tgaccgacat
     1141 caacggcaac ctgatgggca tcaacaccgc gatttattcg cgctcgggcg gcagcatggg
     1201 cattggcttt gccattcccg tcagcacggc caagcaggtg atggagagca tcgtcaagaa
     1261 cggccaggtg gtgcgcggtt ggatcggcgt cgagccgaac gagttgacgc ccgagctggc
     1321 gcagaccttc ggcatccagt tggcgcaagg cgtgatcgtg accggcgtgc tcaacagcgg
     1381 tccggcggcg gaagccggga ttcgccccgg cgacgtcatc acgcaggtgg ccggccagcc
     1441 ggtgcgcacg gtgtcggaat tgctgacgcg catcgcgggc ctgacgcccg gccaggcgac
     1501 gccgtttgcg ctgcagcgcc agggccaggc tgtggaggtg tccgtcacgc ccgcgcagcg
     1561 cccgcggccg cagcgcgtga tgcagcgtta aagcgcgctg attctcactt tttgatagct
     1621 gttcgcgcac gtccggcgtg cgccagaggc caatttgatc ttgaatggag gcgccgtggc
     1681 agcgccccgc ggcttcaaga cttggccgct tccgccgcgt cgtcttcggg cgcgttggtg
     1741 tgcttgatga acagctgcgc ggcccaaatg ccgacctcgt acagcaggca catcggaatc
     1801 gccagcgcca gttgcgacac cacgtcgggc ggcgtgacga tggcggcgat gacgaaggcc
     1861 agcacgatga agtagccgcg aaactgcttc aactgctcga cggtcaccac gcccagccgc
     1921 gccagcacga cgacggcaat cggcacttcg aacgccaggc caaaggccat gaacatggtc
     1981 agcacgaagg tcaggtaggc ctcgatgtcc ggcgcggcgg tgatgctctt gggcgcaaag
     2041 ctctggatga aggtgaacac cttgccaaag acaaagaagt agcaaaacgc cacgcccaga
     2101 aagaacagga tggtgctgga caccaccagc ggcagcacca gcttcttctc atgcgaataa
     2161 agccccggcg ccacgaaggc ccacacctgg tacagcacca ccggcagcgc gaccagaaag
     2221 gccgccatca gcaaaatctt gagcggcacc atgaaaggcg agatgaccga ggtggcgatc
     2281 atcgtcgcgc cggccggcag gtgcgcgatc agcggtgcgg ccagcaggtc gtacagcgcg
     2341 cccgggccgg ggtacagcgc cagcacgccg gccaccacgc ccaccgccac cagcgccttg
     2401 atcagccggt cgcgcagctc catcaggtgc gcgacaaacg gctgctcggt gccggccagc
     2461 tcgtcttcgg gatcggtctt ggggttgggt tcggtcatgg gagaagcgat tgtcgcctgt
     2521 gtggcgatga cgaaatgact tcgccgctag cgcggcttca atgacgaaat gacttggctg
     2581 gacatggcgc gacgacgcgc cgtcatcgag gcccgcgggg ccgaggtcat gtcgtcattt
     2641 gttcttggga cggtagcgcg ccacgcgcgc cgcgcccgac tgcacctggc ggcgcacgcc
     2701 gtggcgcgcc ttgtaccact gcggcgtggc gccgcgcttc aagcgccagt tcttgtcagg
     2761 gcggcggtat tcgggcgccg gcggctgcca ggccggcgca ctgtcgctgg ccgtgccact
     2821 gccgtccagc ccggcggtga cgctgctcca gtccttctcg aaatcggcct tggtggagtg
     2881 aaggcctt
//
LOCUS       DAOMVO010000308         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10297_length_2866_cov_11.675205, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000308 DAOMVO010000000
VERSION     DAOMVO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10297_length_2866_cov_11.675205"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKV30_11955"
     CDS             complement(<1..632)
                     /locus_tag="PKV30_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HRL30960.1"
                     /translation="MADGGAPIWRPDWPAPAHVQACFTTRGTGTADGASAPPCDFFNL
                     GDHVGDAPEAVRANRLRLQHTLRARPVFMNQVHGVGVLHVQPDTPDGATADAAVASQR
                     GLACTVMVADCLPVLLTDTAGRAVAAAHAGWRGLGGGVIEQTLESFRAAALFHQAQAA
                     INSEANQLMAWLGPCIGPQAFEVGAEVREAFVATDAGAAACFTPHAVGKYL"
     gene            complement(632..1273)
                     /gene="maiA"
                     /locus_tag="PKV30_11960"
     CDS             complement(632..1273)
                     /gene="maiA"
                     /locus_tag="PKV30_11960"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRL30961.1"
                     /translation="MKLYSYFRSSASFRVRIALNLKGLAYDYVPVHLPSGAQKKAEFA
                     AVSAEGLVPVLELDDGQRLTQSMAIIEYLDETHPLPAPLLPPEAVGRARVRALAQIVA
                     CEIHPLNNLRVLKYLVNELKTGDDAKNAWYRHWVRLGLQSYESHLAAHPGRVSHGDTP
                     TLADCCLVPQIFNAQRFDCDLSGLPRTMAAFEACMALEAFQKAQPSACPDFEA"
     gene            complement(1346..2053)
                     /locus_tag="PKV30_11965"
     CDS             complement(1346..2053)
                     /locus_tag="PKV30_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRL30962.1"
                     /translation="MTKLIDSGRGLLIAATAAALLTTGCANMTETQRNTGIGAGVGAV
                     AGGLIGDGKGAAIGAAVGAAGGYAWSRYMENKRVQMQQATAGTGVQVTQTPDNQLKLH
                     IPNDISFQTGRADLEPRLRPILDQFAQGLGQQPGTEVFIVGHADSTGSDAINNPLSQQ
                     RANSVRDYLVSRGADSRIIRTEGRGSREPVGSNATDAGRAQNRRVEIFLSERASVAQA
                     PAPAPVPTYNTPVGQPR"
     gene            complement(2179..2625)
                     /locus_tag="PKV30_11970"
     CDS             complement(2179..2625)
                     /locus_tag="PKV30_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRL30963.1"
                     /translation="MSNRFIFEQAPGHLIRRAHQISVAVFADEVGAHAVTPVQFAILN
                     LLLGRPGIDQITLAQGVAFDAATIGSVIGRLEKKGWLRRQPDVADRRRKLLWLTPEGE
                     RAAGALKPLVEGAQQHILEPLSADEQKQLVALLSKLVTHHPRRAAT"
     gene            2673..>2866
                     /locus_tag="PKV30_11975"
     CDS             2673..>2866
                     /locus_tag="PKV30_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRL30964.1"
                     /translation="MHRHIIKHCRACGTAVELRVPDDGDTRERAVCPACGTIHYVNPL
                     LVVGTVPYLGDQVLLCKRAI"
BASE COUNT          447 a          967 c          994 g          458 t
ORIGIN      
        1 aggtatttgc cgaccgcgtg cggtgtgaag caggcggcgg cgccggcgtc ggtggcgaca
       61 aaagcttcgc gcacttccgc ccccacctca aacgcctgcg gcccgatgca ggggccgagc
      121 caggccatca gctgattcgc ttcactattg atagctgctt gcgcttggtg gaaaagcgct
      181 gcagccctga aagactccaa agtctgctcg atcacaccac cgcccaagcc gcgccaaccg
      241 gcgtgcgccg ccgccacggc acggccagcg gtgtcggtca gcagcaccgg caggcagtcg
      301 gccaccatca cggtacaggc caggccgcgt tggctagcga cggcggcatc ggccgtcgcg
      361 ccatcgggcg tgtctggctg cacgtgcaac acgcccacgc cgtgcacctg gttcatgaac
      421 accgggcgcg cacgcagcgt gtgctgcagg cgcaggcggt tggcgcgcac cgcttcgggc
      481 gcgtcgccca cgtggtcgcc cagattgaag aagtcgcacg gcggcgccga agcgccatcg
      541 gccgtgcccg tgccccgcgt ggtgaagcac gcctgcacgt gggcgggtgc cggccagtcc
      601 ggccgccaga ttggcgcgcc gccgtcggcc atcaggcttc gaagtcgggg caggccgagg
      661 gctgcgcttt ttgaaaagcc tccagtgcca tgcaggcctc aaacgcggcc atggtgcgcg
      721 gcaagccgct caaatcgcag tcgaagcgct gggcgttgaa gatctgcggc accaggcagc
      781 aatcggccag cgtgggcgtg tcgccgtggc tgacgcggcc cgggtgcgcc gccagatgcg
      841 actcgtacga ttgcaggccg agacgcaccc agtggcgata ccaggcgttc ttggcgtcgt
      901 cgccggtttt cagctcgttc accaggtact tcagaacgcg caggttgttg agcgggtgaa
      961 tctcgcacgc cacgatctgc gccagggcac gcacgcgcgc gcggcccacg gcctcgggcg
     1021 gcagcaaggg cgcgggcaac ggatgggttt cgtccaggta ctcgatgatc gccatcgact
     1081 gcgtgaggcg ctggccatcg tccagctcca gcaccggcac caggccttcg gcgctgaccg
     1141 cggcaaactc ggctttcttc tgcgcgccgc tcggcaggtg caccggcaca tagtcgtagg
     1201 ccaagccttt caggttcagc gcgatgcgca cgcgaaacga ggcggaggag cggaagtagg
     1261 agtagagctt catgcggcac gactatagcg gcgcaaacaa aaagcgcccc gtggggcgct
     1321 tggtgcggcg gctggacacg cccgatcagc gcggctggcc gaccggcgtg ttgtaggtcg
     1381 gcaccggcgc cggtgcgggc gcctgcgcca cgctggcgcg ctcggacagg aagatctcga
     1441 cgcggcggtt ctgcgcccgc ccggcgtcgg tggcgttgct gcccaccggc tcgcgcgagc
     1501 cgcggccttc ggtgcggatg atgcgcgaat cggccccgcg cgagaccagg taatcgcgca
     1561 cgctgttggc gcgctgctgc gacagcgggt tgttgatggc gtcactgccg gtcgaatccg
     1621 catggccgac gatgaacacc tcggtgcccg gctgctggcc caggccttgc gcaaactggt
     1681 cgaggatcgg gcgcagccgc ggctcaaggt cggcgcggcc ggtctggaaa gagatgtcgt
     1741 tcgggatgtg cagcttcaac tggttgtcgg gcgtctgcgt gacctgcacc ccggtgccgg
     1801 cggtggcctg ctgcatctgc acgcgcttgt tttccatgta acgcgaccag gcgtagccgc
     1861 ccgccgcgcc gacggcagca ccaatggcgg cgcccttgcc gtcgccaatc agtccgccag
     1921 ccacggcgcc cacgccagca ccgatgccgg tgttgcgctg cgtttcggtc atgttggcgc
     1981 agccggtggt cagcagcgcg gccgcggtgg cggcaatgag caggccacgg cctgagtcga
     2041 tgagtttggt catggaagtc gtcctttcgt attctgatcg gccatgagca cggcctgtgt
     2101 gacgcgacgc tgtgcggcgc gctttgcatt taacgagcga cgtcggcgac aacctgtagg
     2161 acgcggcctc aaacccgttc aggtggcggc gcggcgcggg tgatgtgtga ccaatttgga
     2221 caacagcgcc accagctgct tctgctcatc ggcgctgagc ggctccagaa tgtgctgctg
     2281 cgcgccctcg accagcggct tcagcgcgcc ggccgcccgt tcgccctcgg gcgtgagcca
     2341 caacagcttg cggcgccggt cggccacatc gggctggcgg cgcagccagc cttttttctc
     2401 cagccggccg atcaccgagc cgatggtggc ggcatcaaac gccacgccct gcgccagcgt
     2461 gatctggtcg atgccgggcc ggccgagcag caggttgagg atcgcgaact gcaccggcgt
     2521 gactgcgtgc gcgccgacct catcggcaaa caccgcgacc gagatttggt gcgcgcgccg
     2581 gatcaggtgc cccggcgcct gctcgaaaat gaagcggttg gacatggcga cgagtgtagc
     2641 gatgcgtttt tagcccacta cactgacgcc caatgcaccg ccacatcatc aagcactgcc
     2701 gcgcctgcgg caccgccgtg gagcttcgcg tgccggacga cggcgacacg cgtgagcgcg
     2761 ccgtgtgccc cgcctgcggc accatccact acgtcaaccc gctgctggtg gtgggcaccg
     2821 tgccttacct gggtgaccag gtgctgctgt gcaagcgcgc catcga
//
LOCUS       DAOMVO010000309         2853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10352_length_2853_cov_13.858113, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000309 DAOMVO010000000
VERSION     DAOMVO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10352_length_2853_cov_13.858113"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKV30_11980"
     CDS             complement(<1..362)
                     /locus_tag="PKV30_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL30965.1"
                     /translation="MTDHSPPPSGADRAFGFATRAIHAAAVPDPVTGARATPIHQSTS
                     FVFDSAEHASSLFNLQTFGNVYSRISNPTVAVFEERMAALENGRAALACASGMAAQMT
                     ALLAILKAGDHIVAASTL"
     gene            complement(592..1212)
                     /locus_tag="PKV30_11985"
     CDS             complement(592..1212)
                     /locus_tag="PKV30_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRL30966.1"
                     /translation="MNTLPSRTLLSAAALLVLGAHLPARAARVGQPAPDFSLPDTGGK
                     AVRLADFRGRFVVLEWTNPGCPFVRKHYESGNMGATQKLARDQGVAWLAINSTETASG
                     DYLEPPRLADWLKQHQGVPTAVLMDPEGTTGRAYDAKTTPHLYIVDPQGMLVYAGGID
                     SIASARVSDIEKATNHVKAALADIAAKRPIAQAVTKPYGCTIKYKT"
     gene            complement(1229..1744)
                     /locus_tag="PKV30_11990"
     CDS             complement(1229..1744)
                     /locus_tag="PKV30_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRL30967.1"
                     /translation="MARTPDTISTLRRVLNECRTLAVVGLSPQWHRPSFFAAKYMQTH
                     GFRIVPVNPLVAREGGRILGERAWASVREADAAIRAEGGRIDMVDCFRKSEDIPPLAD
                     DAVAIGAKCLWLQLGVVNENAARAAEDAGLLVIQNRCVKIEHARLFGGLGWAGVNTKV
                     ISSKRPQVLPY"
     gene            2023..>2853
                     /locus_tag="PKV30_11995"
     CDS             2023..>2853
                     /locus_tag="PKV30_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL30968.1"
                     /translation="MDQHYLTPLFEPKSIVLFTGPQGAPEQQTPQARALLAHMAAQPF
                     KGQVTPLDIHTTGTLADLAQARADLAIIALPEDEIVPALELAGRIKVKAALVLSAGIP
                     QAKAAEMHRVAKQHGMWLLGPNSQGFQRPMLQLNASSAGDLATQGQLALVSQSGALTA
                     SILDWARQNRVGFSNVIALGAHTQVGLAGVLDYLANDRHTQGIVVYMEGIANARRFTS
                     ALRAAANSKPVVVLKAGRKQDGNAAAQTHSGAIVGSDEVWGAVLRRAGAVRVRSFVEL
                     F"
BASE COUNT          425 a          945 c         1040 g          443 t
ORIGIN      
        1 tagagcgtgc tggcggccac gatgtggtcg ccggccttca ggatggccag cagcgccgtc
       61 atctgcgccg ccatgccgct ggcgcaggcc agcgcggcgc ggccgttttc cagcgcggcc
      121 atgcgctcct cgaacacggc caccgtcggg ttgctgatgc ggctgtagac gttgccgaag
      181 gtctgcaggt tgaacaggct ggaggcgtgc tcggcgctgt cgaacacgaa gctggtgctc
      241 tggtggatcg gcgtggcgcg cgcgccggtc accggatcgg gcacggcggc ggcgtggatg
      301 gcgcgggtgg cgaagccgaa ggcgcggtcg gcgccggatg ggggtgggct gtggtcggtc
      361 attcggtttg gaaaacagac ggtcggtagg tcacgaaacg cggctggccc tcacccctgc
      421 cctgccgcca gcgggagagg gggcaagacc cggcagcgcg gccagcgatt cggccggccg
      481 cggagcaggc catgccagcg aacgccatgc ccggacctgc gccgggcatg gcgttgtccc
      541 ccctcgcgca gcagaggggg gaaggcgccg caggcgactc aggggggtgc atcaagtctt
      601 gtacttgatg gtgcagccgt acggcttggt gaccgcctgc gcgatcgggc gcttggccgc
      661 gatgtcggcc agcgccgcct tcacgtggtt ggtcgctttc tcgatgtcgg acacgcgtgc
      721 cgaggcgatg ctgtcgatgc cgcccgcgta caccagcatg ccctgcgggt cgacgatgta
      781 caggtgcggc gtggtcttgg cgtcgtaggc gcggccggtg gtgccctcgg ggtccatcag
      841 cacggcggtg ggcacgccct ggtgctgctt cagccagtcg gccagcctgg gcggttccag
      901 atagtcgccg ctggcggttt cggtggagtt gatggccagc cacgccacgc cctggtcgcg
      961 cgccagcttc tgcgtggcac ccatgttgcc gctttcgtag tgcttgcgca cgaaggggca
     1021 gccggggttg gtccactcga gcaccacgaa acggccgcgg aagtcggcca gtcgcaccgc
     1081 tttgcccccg gtgtcgggca ggctgaagtc gggcgcgggc tggcccacgc gggcggcgcg
     1141 ggcgggcagg tgcgcgccca gcacgagcag ggccgcggcg gagagcaggg tgcggctggg
     1201 cagggtgttc atggcaaagc tcctgtcgtc agtaggggag aacctgcggg cgcttggacg
     1261 agatgacttt cgtgttcacc cccgcccagc ccagcccgcc gaacaggcgc gcgtgctcga
     1321 tcttcacgca gcggttctgg atcaccaaca ggccggcgtc ctcggccgcg cgcgcggcat
     1381 tctcgttgac cacgccaagc tgcagccaca ggcacttcgc gccaatggcc acggcgtcgt
     1441 cggccagcgg cgggatgtcc tcgctcttgc ggaagcagtc caccatgtcg atgcgcccgc
     1501 cctcggcgcg gatggcggcg tcggcctcgc gcacgctggc ccaggcgcgt tcgcccagga
     1561 tgcggccgcc ctccctggcc accagcggat tcaccggcac gatgcgaaag ccgtgcgtct
     1621 gcatgtactt ggcggcaaag aagctcggcc ggtgccattg cggcgacagg ccaaccactg
     1681 ccagcgtgcg gcactcgttc agcacgcggc gcagggtgga gatggtgtcg ggggtgcggg
     1741 ccatgggcgc agtgtacgca ggccggggcg gtggcgtgca gcggccccga tttcaagcca
     1801 aatcggcctt caggcgccgc ccatcaagcg ctgatagcta ctgtattaat agtaaaaagt
     1861 cgatgaaaca cctgttgctg cggcgcatcg tggcctgccg cgcagcggga ttgatgcacg
     1921 gccgtcgctg cttcttgatc aaaacccgtt agtctgtaag ggaaattgtg cccagaatac
     1981 gggttaaccc ttatcactag cgcttcggcg catgcaacgc ccatggacca gcactacctc
     2041 acccccctgt tcgagcccaa gtccatcgtc ctgttcaccg gcccgcaagg cgcgcccgag
     2101 cagcagacgc cgcaggcgcg cgcgctgctg gcgcacatgg cggcgcagcc cttcaagggc
     2161 caggtcacgc cgctggacat ccacaccaca ggcacgctgg ccgatctggc gcaggcgcgg
     2221 gccgatctgg ccatcatcgc gttgcccgag gacgaaatcg tgcccgcact ggagctggcc
     2281 gggcgcatca aggttaaagc ggcgctggtg ctgtcggccg gcatcccgca ggccaaggcg
     2341 gccgagatgc accgcgtggc caagcagcac ggcatgtggc tgctggggcc caacagccag
     2401 ggcttccagc ggccgatgct gcagctgaac gccagctcgg ccggcgacct ggccacgcag
     2461 gggcagctgg cgctggtgtc gcaatccggc gcgctcacgg cctcgatcct ggattgggcg
     2521 cggcagaacc gcgtcggctt ttccaacgtc atcgccctgg gcgcgcacac gcaggtgggg
     2581 ctggccggcg tgctggacta cctggccaac gaccgccaca cgcagggcat cgtggtgtac
     2641 atggagggca ttgccaacgc ccggcgcttt accagcgcgc tgcgcgcggc ggccaacagc
     2701 aagccggtgg tggtgctgaa ggccggccgc aagcaggacg gcaacgcggc cgcgcagacg
     2761 cactcgggcg ccatcgtcgg cagcgacgag gtgtggggcg ccgtgctgcg ccgcgccggc
     2821 gcggtgcgcg tgcgctcttt cgtcgagctg ttc
//
LOCUS       DAOMVO010000310         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10410_length_2841_cov_11.618808, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000310 DAOMVO010000000
VERSION     DAOMVO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_2841_cov_11.618808"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1457)
                     /locus_tag="PKV30_12000"
     CDS             complement(123..1457)
                     /locus_tag="PKV30_12000"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRL30969.1"
                     /translation="MILALYSLATWLAQPLLRRKLRRRGVAEPGYLRAVDERFGRYTT
                     ARPDGRGPLVWIHAVSLGETRAAAILLAALRERLPTMRLLLTHGTATGRAEGQKLLGA
                     GDVQVWQPWDTRGATRRFLTHFKPDVGLLMETEIWPNQMHEAAARGVPMVLANARLSE
                     KSLRGAQRAGLLLRPAYCALTAAWAQSEADAARLRTIGVRQVDVLGNLKFDARPDAAQ
                     QALGARWRAALGGRPVVMLASSREGEEVELLEQIKALALDGQARAAIKNEVKAMSAPL
                     WLIVPRHPQRFAEVADLARAAGFEVAQRSRWADAPSPDALTADVWIGETLGEMTLYYS
                     LADVALLGGSFAPLGGQNLIEAAACGCPVVMGPHTFNFAQAAELAEAAGAARRVGDMA
                     AGVAAALALSQRGPAHAAMSRAALDFAGAHQGAAARTADALAAVLERTKGLP"
     gene            complement(1523..>2841)
                     /locus_tag="PKV30_12005"
     CDS             complement(1523..>2841)
                     /locus_tag="PKV30_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRL30970.1"
                     /translation="VAEAIGLAFGTQAMALGERVVAVGRDGRLSGPALSAALMRGLVA
                     AGVEVIDIGLATTPMLYFAAHTLCTSGIQVTGSHNPRDDNGFKMVLAGRAIYGDDIQA
                     LRRMIETDGWHKQAGGSVRTHDVLPAYRERIVGDIKMARPMKVVVDCGNGVAGASAPG
                     IFRAIGCEVIELFSEVDGNFPNHHPDPSKPDNLRDVIRVLQDTDAELGLAFDGDGDRL
                     GIVTKDGQNIFPDRQMMLFAQDVLARVPGGTIVYDVKCSQRLAPAIEAAGGVPLMFKT
                     GHSLVKARMKEVDSPLGGEMSGHIFFKERWYGFDDGTYAGCRLLEILSRHADPSAVLN
                     QLPTSHSTPELNVACAEGEPHRLAAELQGLAADAFKAPAQVSTIDGLRVDWPDGFGLI
                     RASNTTPVLVLRFEGQTDAALKRIEADMLALLRRVKPEAQLGASAH"
BASE COUNT          428 a          985 c          984 g          444 t
ORIGIN      
        1 atctccacgc cgaacagcac ggcctggatg acgaggaaga tggcgaagaa gcgaagcacg
       61 gttcgggcag cctgtgaaga ggaactcgcg ccgactctag caggaccgtg tgcctgaatg
      121 tttcagggca agcccttggt gcgttccaac acggccgcca gcgcatcggc ggtgcgcgcg
      181 gcggcgccct gatgcgcgcc agcaaagtcc agcgcggcgc gcgacatggc tgcgtgggcg
      241 gggccgcgct gcgacaaggc cagcgcggcg gcgacgccgg cagccatgtc gcccacgcgc
      301 cgcgccgcgc ccgcggcttc ggccagttcg gccgcctgcg cgaagttgaa ggtgtgcggc
      361 cccatcacga ccgggcagcc gcaggcggca gcttcgatca ggttctggcc tcccagcggc
      421 gcgaagctgc cgcccagcaa ggcgacgtcg gccaggctgt agtagagcgt catctcgcct
      481 aaggtctcgc cgatccagac gtcggcggtc agcgcgtcgg gcgacggcgc atcggcccag
      541 cgactgcgtt gcgccacctc gaagccggcc gcgcgcgcca ggtcggcgac ttcggcaaat
      601 cgctgcgggt ggcgcggcac gatcagccag agcggcgcgc tcatcgcttt gacttcgttt
      661 ttgatagctg cccgcgcttg tccgtcaagc gccagcgcct tgatttgttc aagaagctcg
      721 acctcctcgc cctcgcgcga gctggccagc atgaccaccg ggcgaccacc cagcgcggca
      781 cgccagcgcg cgcccaacgc ctgctgcgcc gcgtcgggcc gggcgtcgaa cttcaggttg
      841 cccagcacat cgacctggcg cacgccgatg gtgcgcaggc gcgcggcgtc ggcctcggac
      901 tgcgcccagg cggcggtcag cgcgcagtag gcggggcgca acaacaggcc ggcgcgctgg
      961 gcaccgcgca gcgatttctc ggacaagcgg gcgttggcca gcaccatggg cacgccgcgc
     1021 gcggcggctt cgtgcatctg gttgggccag atctcggttt ccatcagcaa gccaacgtcg
     1081 ggcttgaagt gcgtgagaaa gcgccgcgtg gcgccgcgcg tgtcccacgg ctgccagacc
     1141 tggacgtcgc ccgcgcccag cagcttctgc ccttcggcgc ggccggtggc ggtgccgtgc
     1201 gtcagcagca gacgcattgt cggcaagcgc tcgcgcaacg cggccagcag gatggcggcg
     1261 gcgcgtgttt cgcccagcga cacggcgtga atccagacca gcgggccgcg cccatcgggc
     1321 cgcgccgtgg tgtaacggcc aaagcgctcg tccaccgcgc gcagatagcc cggctcggcc
     1381 acgccgcgcc ggcgcagctt gcggcgcaac agcggttgcg cgagccaggt ggccaggctg
     1441 tagagcgcga ggatcatcgc gcgctatcaa aaacaatagc ggcttgcgct tgctgcatca
     1501 gcgctggcgg ctttttttgc attcagtgtg cggatgcgcc aagttgcgcc tcgggcttga
     1561 cgcgtcgcag cagcgccagc atgtcggctt cgatacgttt cagcgccgca tcggtctgcc
     1621 cctcgaagcg cagcaccagc accggcgtgg tgttgctggc gcggatcagg ccgaagccgt
     1681 cgggccagtc gacgcgcagg ccgtcgatgg tgctcacttg tgcgggtgcc ttgaacgcgt
     1741 cggcggccaa gccttgcaac tcagccgcca ggcggtgcgg ctcgccttcc gcgcaggcca
     1801 cgttcagctc gggcgttgaa tggctggtgg gcagttggtt gagtacggcg ctggggtcgg
     1861 cgtgacggct caggatttcc agcaggcggc agccggcgta ggtgccgtcg tcgaagccgt
     1921 accagcgctc cttgaagaag atgtggccgc tcatctcgcc gcccaggggc gagtcgacct
     1981 ctttcatgcg cgccttgacc agcgagtggc cggtcttgaa catcagcggc acgccgcccg
     2041 cagcttcaat ggcgggcgcc agacgctgcg agcatttcac gtcgtagacg atggtgccgc
     2101 ctggcacgcg ggcgagcacg tcttgcgcga acagcatcat ttgccggtcg gggaagatat
     2161 tctgcccgtc cttggtgacg atgcccagcc ggtcgccgtc gccgtcgaag gccaggccca
     2221 gctcggcgtc ggtgtcttgc agcacgcgga tcacgtcgcg caggttgtcg ggcttgctcg
     2281 ggtcggggtg gtggttgggg aagttgccat cgacctcgct gaacagctcg atcacttcgc
     2341 agccaatcgc gcggaagatg cccggcgccg atgcgcccgc cacgccgttg ccgcaatcga
     2401 ccacgacttt catcggccgc gccattttga tgtcgcccac aatgcgctcg cggtacgcgg
     2461 gcagcacgtc gtgcgtgcgc acgctgccgc cagcttgctt gtgccagcca tcggtttcga
     2521 tcatgcggcg cagcgcctga atgtcgtcgc cgtaaatcgc gcgcccggcc agcaccatct
     2581 tgaagccgtt gtcatcgcgc gggttgtggc tgcccgtgac ctgaatgccg ctggtgcaca
     2641 gcgtgtgtgc ggcaaagtac agcatcggcg tggtggccag gccgatgtcg atcacctcca
     2701 cacccgccgc gaccagcccg cgcatcagcg ccgccgacag cgccgggccc gacaggcgcc
     2761 catcgcgccc cacggcgacc acgcgttcgc ccagcgccat cgcctgcgtg ccgaaagcca
     2821 gcccgatggc ctcggccacc g
//
LOCUS       DAOMVO010000311         2832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10457_length_2832_cov_16.208138, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000311 DAOMVO010000000
VERSION     DAOMVO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10457_length_2832_cov_16.208138"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKV30_12010"
     CDS             complement(<1..554)
                     /locus_tag="PKV30_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRL30971.1"
                     /translation="MPPAVDGPRSSPSTDALPPLAALSAHVEALAARGAERRFRAGTL
                     IIQEGERGDTLYIVRSGRLRAFVADNSGKELTLGLYGPGEYVGEMSLDGGPRSASVEA
                     AEPTTCAVITRDVLMTYIAEQPGFALELMARLIRRARLATDNARSMALVDVYGRLTRL
                     LDQLAEPPDADGARTLRERLTHQQ"
     gene            complement(561..1265)
                     /locus_tag="PKV30_12015"
     CDS             complement(561..1265)
                     /locus_tag="PKV30_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRL30972.1"
                     /translation="MQAVATVRERERNQAPVETAKADDLHPSFLLRLLDEIDYGLVLV
                     NAQGRVQHANHLARYELATGRLLHSGPDGQIGTAAAPQTEQLMAAIHGAVQGRRRLLY
                     LTHGEHNMPLAVIPLVHALEGPASSVLLVMARQRAGDNLALQMFAREHSLTPTEESVL
                     RALCDGRDVDEIAIQHGVAESTVRTQVRSLRDKTGANGIRQLVQRVLALPPVVPALRG
                     NGSPQRASALSGFQFV"
     gene            complement(1446..2093)
                     /locus_tag="PKV30_12020"
     CDS             complement(1446..2093)
                     /locus_tag="PKV30_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL30973.1"
                     /translation="MKTAPRYLTVPVTPFQQNCSIVWCPRTMEAAVIDPGGDLPRIEA
                     AVQQLGVQLKQIWLTHAHIDHAGGTAELAERLGLPIVGPHPGDQFWIDGIAEQGRMFG
                     FPQVEKFSPTRWLHDGDTVQVGDWVFNVRHCPGHTPGHVVFHSPEAERAFVGDVLFAG
                     SIGRTDFPGGNHAELIASIRERLWPMGDDTVFIPGHGPESSFGQERRHNPFVRGG"
     gene            complement(2165..2497)
                     /locus_tag="PKV30_12025"
     CDS             complement(2165..2497)
                     /locus_tag="PKV30_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30974.1"
                     /translation="MFPQEWRPILQSMVQIYPQGCNLGLIGRSTDLDTYVIKRTVRDL
                     TARGFIALDASDKDGRMLRLTEAGMAVACDRAANPEAPEVVSALEFDALQEMSRCRAQ
                     RSGFGRLN"
     gene            complement(2715..>2832)
                     /locus_tag="PKV30_12030"
     CDS             complement(2715..>2832)
                     /locus_tag="PKV30_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRL30975.1"
                     /translation="YHLATSAVAAAARHSAIYKDEKFSDHAPITIDYDLKLI"
BASE COUNT          434 a          899 c         1011 g          488 t
ORIGIN      
        1 atttgctggt gcgtcaggcg ctcgcggagc gtgcgcgcgc cgtcggcgtc gggcggctcg
       61 gccagctggt caagcaggcg cgtgaggcgg ccatacacgt cgaccagcgc catgctgcgc
      121 gcgttgtcgg tcgccaggcg ggcgcggcgg atcaggcgcg ccatcagttc cagcgcgaaa
      181 ccgggctgct cggcgatgta ggtcatcagg acatcacgcg tgatgacggc gcacgtggtg
      241 ggttcggcag cctcgacgct ggccgagcgc gggccgccgt caagcgacat ctcgcccacg
      301 tattcgccgg ggccgtacag gccgagggtg agttccttgc cgctgttgtc ggccacgaag
      361 gcgcgcagcc ggccggagcg cacgatgtag agcgtgtcgc cgcgctcgcc ttcctgaatg
      421 atcagggtac cggcgcgaaa acgccgttcg gcgccgcgcg cggccagcgc ctcgacgtgg
      481 gcagacaggg ccgccagcgg cggcagggcg tcggttgaag gggatgaacg cgggccgtca
      541 acggcggggg gcatgcccca tcatacaaat tgaaacccgc tcagggctga cgcgcgctga
      601 gggctgccgt tgccgcgcag ggccggcacc acgggcggca gcgccagcac gcgctgcacc
      661 agctggcgga tgccgttggc accggtcttg tcgcgcagcg aacgcacttg ggtgcgcacg
      721 gtggattcgg ccacgccgtg ctggatggcg atttcgtcga cgtcgcggcc gtcgcacagg
      781 gcgcgcagca cggattcctc ggttggcgtc aggctgtgtt cgcgggcaaa catctgcagc
      841 gccaggttgt cgccggcccg ctggcgcgcc atgaccagca gcacgctgct ggccggccct
      901 tcaagcgcgt gcaccagcgg gatgacggcc agcggcatgt tgtgctcgcc atgcgtcagg
      961 tacagcaggc gccggcggcc ttgcacggcg ccgtggatgg cggccatgag ctgctcggtc
     1021 tgcggcgcgg cggcggtgcc gatctggccg tcgggcccgc tgtgcagcag gcggccggtg
     1081 gccagttcgt agcgcgccag gtggttggca tgctgcaccc ggccttgtgc gttgaccagt
     1141 accagcccgt agtcgatctc gtccagcaac cgcagcaaaa agctggggtg caggtcgtcg
     1201 gcctttgcgg tctcgacggg tgcctggttt ctctccctct ccctgactgt agcgacagct
     1261 tgcatttcat cctccgtggc cgggttgcgg catgggggaa agtttaggca cggctttcgg
     1321 tcgctaccag tgattgacta acacgcgctt tgtgatccat tcacatccgc ttggcctgcg
     1381 ctgcaccgcg tgcaacatgt ccttgcaatg cgtcgcgcgc gccacgccgg cgtgcgaaat
     1441 gcccgtcagc cgccgcgcac gaaggggttg tggcgccgct cctggccaaa gctgctttcc
     1501 ggcccgtggc cggggatgaa caccgtgtcg tcgcccatcg gccacaggcg ctcgcggatg
     1561 ctggcgatga gctcggcgtg gttgccgccg gggaagtcgg tgcggccgat gctgccagca
     1621 aacagcacgt cgccgacgaa ggcgcgctcg gcttcgggcg aatgaaacac gacgtggccc
     1681 ggtgtgtggc cggggcaatg ccgcacgttg aacacccagt cgcccacctg caccgtgtcg
     1741 ccgtcgtgca gccagcgcgt ggggctgaat ttttcgacct gcggaaagcc gaacatgcgc
     1801 ccctgctcgg cgatgccgtc gatccagaac tggtcgcccg ggtgcgggcc gacgatgggc
     1861 aagccgagcc gctcggccaa ctccgccgtg ccgcccgcgt ggtcgatgtg ggcgtgggtg
     1921 agccagattt gcttcagttg caccccgagc tgctgcaccg ccgcctcgat gcgcggcaga
     1981 tcgccgcccg ggtcgatgac ggccgcttcc atcgtgcgag ggcaccagac gatggagcag
     2041 ttttgctgaa acggcgtgac gggaacggtc aggtagcgcg gagcggtttt catgggatgg
     2101 gtgttctaca ccaagtgaac accccgccct gaatatgcgc cgcgggcgcg gcgcttgggg
     2161 ccgctcagtt cagccggcca aagccggagc gctgggcgcg gcagcgcgac atttcctgca
     2221 gcgcgtcaaa ttccagcgcg ctcaccactt cgggcgcttc gggattggcc gcacggtcgc
     2281 aggccacggc catgccggct tcggtcaggc gcagcatgcg gccgtccttg tcgctggcgt
     2341 ccagggcaat gaagccgcgc gccgtgaggt cgcgcacggt gcgcttgatg acgtaagtgt
     2401 cgaggtcggt cgagcgaccg atcagcccca gattgcagcc ttgcgggtag atctggacca
     2461 tcgattggag gatgggtctc cattcctggg gaaacatggc acgtgcctcc tgttcgttaa
     2521 gggcagcgtt gcttcttgga gctccctggc gtggcggcgg cgcattgaaa gggcgcccgg
     2581 ccagtcagcc cactataggc cggaatggcc gtggcgtgtg aggcccttcg cgaatccgag
     2641 gccatgaaat ttgaatgaaa acagcctgca gcgcttgtcc atcaagcgcg agaagctatg
     2701 ctaaaaatag caaatcagat cagcttcagg tcgtaatcaa tggtgatcgg cgcgtggtcg
     2761 ctgaacttct cgtccttgta gatcgcgctg tggcgcgcgg cggcggccac ggcagatgtc
     2821 gccaggtggt ag
//
LOCUS       DAOMVO010000312         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10467_length_2830_cov_13.067748, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000312 DAOMVO010000000
VERSION     DAOMVO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10467_length_2830_cov_13.067748"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKV30_12035"
     CDS             <1..641
                     /locus_tag="PKV30_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HRL30976.1"
                     /translation="ALILLDIAAEPAKDGEAELEPVRIVEGDSAPTRYGDQADGADDA
                     PARAQDQAGARAARRETPVPVVLTRFAAQNLYAPLRTVEPLPGVMRVNLRRDLDLGTL
                     VPTLPVRAVALASWRLEDQWVTAVRLTNSSGGWITLDPRVLQGDFLTATFQHEALGPR
                     GTPEDTTVLYLVTRGHGLAQSLLPAINRFDPAVHLPQPEAEATDDKEARHAQ"
     gene            631..2061
                     /locus_tag="PKV30_12040"
     CDS             631..2061
                     /locus_tag="PKV30_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HRL30977.1"
                     /translation="MRSNGLLKWLMIPVALLVLFVAIRLFSGGGSSAPPAADAGAKLT
                     PEEMKALGIEGDTPRDTVATLVAQVKQLRTELQTALSDNKSQREENQRLRQRENSIDQ
                     RINSALETERSNLRRDQQQAASERQQTEGLLADLQRRLEGIGGRGGGHADLPVGLGLR
                     NGDEAGMEGGVRWVEPDDAKPADGRNGSRGTGGGMSFPTSFGPAQSTLETTAQTVANA
                     GARAAGVKSAKPVYTVPTNSTLMGSVAMTALIGRVPIDGTVNDPYPFKVLVGPDNLTA
                     NGIDIPDVAGAVFSGTASGDWTLSCVRGQVRSITFVFHDGTIRTIPEDRDGNQQNNQQ
                     QNSQSGGLGWISDPYGIPCVSGERRSNAQQYLGSQALITAAGAGVASLIDSDSGQMSY
                     VGADGSIGSVGISGNEAVGRILAGGVRDMADWVNKLYGQAFAAVYVQPGAKVAVHLEK
                     PLAIDFDPEGRKVDHRAGESHALELE"
     gene            2042..2482
                     /locus_tag="PKV30_12045"
     CDS             2042..2482
                     /locus_tag="PKV30_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HRL30978.1"
                     /translation="MLSNLNKGLALALAVAVLGGCATSKEKLLPHGDSTMMDIWQQNA
                     GDGGGGAGQVARRQLLDARQSLRRPLTEMDVQAAPAEQMRYTRTARNEVYRQFQRLPN
                     PDLVMYVYPHLAGTDPVPVPGYTTVFPLYQRVQYAMPGERVEDY"
     gene            2482..>2830
                     /locus_tag="PKV30_12050"
     CDS             2482..>2830
                     /locus_tag="PKV30_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HRL30979.1"
                     /translation="MRWKLPWHKSAAPKLAASGAGDDERPEGWQRHVEALRQAGIPEP
                     GTAVHGRRPATVADEQALYDVAPSFAEFLPWVEFLPDSKSMLLEDGQSVAAFYELVPL
                     GTEGREPGWLAHAR"
BASE COUNT          494 a          966 c          974 g          396 t
ORIGIN      
        1 gcgccttgat cctgctggac atcgctgccg agccggccaa ggacggcgaa gccgagttgg
       61 aaccggtgcg catcgtcgag ggcgacagcg ccccgacgcg ctatggcgat caagccgacg
      121 gagcagacga cgccccggcg cgcgcccagg accaggcagg cgcgcgggca gcgcggcgtg
      181 aaaccccggt tccggtcgtg ctgacgcgct tcgccgcgca gaacctctac gcaccgctgc
      241 gcaccgtgga gccgctgccg ggcgtcatgc gggtgaacct gcgccgcgat ctcgacctcg
      301 gcacgctggt gccgacgctg ccggtgcgcg cagttgcgct cgcctcgtgg cgtctggaag
      361 accagtgggt cacagccgtg cgcctcacca atagcagcgg cggctggatc accctggacc
      421 cgcgcgtgtt gcagggcgat ttcctcaccg ccaccttcca gcacgaagca ctcggcccgc
      481 gcgggacgcc agaggacacg accgtcctgt acttggtgac gcgggggcac ggcctcgcgc
      541 aatcgctgct gccggcgatc aaccgcttcg acccagccgt gcatctgccg cagcccgaag
      601 ccgaggccac cgacgacaag gaggcccgcc atgcgcagta acggactcct gaagtggtta
      661 atgatccccg tggccctgct ggtgctgttc gtcgccatcc ggctgttttc gggtggaggc
      721 tcgtcggcgc cgcccgctgc ggatgccggc gccaagctca cgccggagga gatgaaggcg
      781 ctgggcatcg agggcgatac cccccgcgat accgtggcga cgctcgtggc gcaagtgaag
      841 cagttgcgca ccgaactgca gacggcgctg tccgacaaca agtcccagcg cgaagagaac
      901 cagcgcctgc gccaacgcga gaactcgatc gaccagcgca tcaattcggc gctggagacc
      961 gagcgctcca acctgcgccg cgaccagcag caggcagcca gcgaacgcca gcagaccgag
     1021 ggactgctcg ccgacctgca gcggcgcctg gaaggcatcg gcgggcgcgg cggcggccac
     1081 gccgatctac ccgtgggcct ggggctgcgt aacggcgacg aggcaggcat ggaaggcggc
     1141 gtgcgctggg tcgagccgga cgacgcgaag cccgctgatg ggcgtaacgg cagtcgcggc
     1201 acgggtggcg gcatgagctt tccgacgagc ttcggccccg cgcagagcac gctggaaacc
     1261 acggcacaaa ccgtggcgaa cgcgggcgcc cgcgccgcag gcgtcaagag cgccaagccg
     1321 gtctatacgg tgccgaccaa ctcgacgctg atgggatcgg tggcaatgac ggcgctgatc
     1381 ggccgcgtgc cgatcgacgg tacggtcaac gatccgtatc cgttcaaagt cctggtcggt
     1441 cccgacaatc tcacggcaaa cggtatcgac attcccgacg tggcgggcgc cgtgttttcc
     1501 ggcacggcct cgggcgactg gacgctctct tgcgtgcgcg gccaggtgcg cagcatcacc
     1561 tttgtgttcc atgacgggac cattcggacg attcccgaag accgcgacgg caaccagcag
     1621 aacaaccagc agcagaactc tcaaagtggg ggcctgggct ggatcagcga cccgtatggc
     1681 attccttgcg tcagcggaga gcggcgcagc aacgcccagc agtacctcgg ctcacaggcg
     1741 ctgatcaccg cggcgggtgc cggcgtcgcc tcgctcatcg acagcgacag cggtcagatg
     1801 tcctacgtgg gcgccgatgg ctccatcgga agcgtcggca tctcgggcaa cgaagccgtg
     1861 ggccgcatcc tggccggcgg cgttcgggac atggccgatt gggtgaacaa gttgtacggc
     1921 caggccttcg ccgccgtcta tgtccaaccc ggcgcaaagg tcgccgtcca cctcgaaaag
     1981 ccgctcgcca tcgatttcga tcccgaaggt cgcaaggtcg atcaccgtgc aggagaaagc
     2041 catgctctcg aacttgaata agggcctggc gctggccctt gccgtcgcgg tgctcggcgg
     2101 ctgcgccacc agcaaggaaa agctgctgcc ccacggcgac agcacgatga tggacatctg
     2161 gcagcagaac gccggtgacg gcggcggtgg cgccggccag gtggcacgca ggcaattgct
     2221 cgacgcgcgc cagagcctgc gccggccgct gaccgagatg gatgtacagg ccgcgcccgc
     2281 cgagcagatg cgctacacgc gcacagcgcg caacgaggtc tatcgccagt tccagcgcct
     2341 gccgaatccc gacctggtga tgtacgtgta cccgcacctg gcaggcacgg acccggtgcc
     2401 cgtgccgggc tatacgacgg tttttccctt gtaccagcgc gtgcagtacg ccatgccggg
     2461 cgagcgcgtg gaggactact gatgcgctgg aaactcccct ggcacaagtc ggccgcgccg
     2521 aagctggccg catccggcgc aggcgatgac gagcggccgg agggctggca acgccacgtc
     2581 gaggccttgc gccaggccgg catcccagaa cccggcacgg cggtccatgg ccgcaggccg
     2641 gcgaccgtgg ccgacgagca ggcgctgtac gacgtcgcgc cgtcgttcgc ggaattcctg
     2701 ccctgggtgg agttcctgcc cgattcgaag tccatgctgc tggaagacgg gcaatcggtc
     2761 gcggcattct acgagctggt gccgctgggc accgagggcc gggaacccgg ctggctcgcg
     2821 catgcccgcg
//
LOCUS       DAOMVO010000313         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10650_length_2798_cov_15.972293, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000313 DAOMVO010000000
VERSION     DAOMVO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10650_length_2798_cov_15.972293"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKV30_12055"
     CDS             <1..422
                     /locus_tag="PKV30_12055"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903338.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRL30980.1"
                     /translation="RHGLRMGTSEIYSAVEGIADVLDSMVVDLEYLGRDSYMPLFVVL
                     RPGVELDEALKGRIAGAIRQSLSPRFVPDDIFQVAEIPRTLSGKKQELPIKKLLLGQP
                     LEKVVNRDAMANPASLTWFAEFAAQRASASKSGAASA"
     gene            419..751
                     /locus_tag="PKV30_12060"
     CDS             419..751
                     /locus_tag="PKV30_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30981.1"
                     /translation="MTHKRQATIGHEYPCFAGDGEAARMMRATDWAATPLGPVEGWPP
                     GLGSIVATLLGSRQPMFLWWGRELVQFYNDAYIPSLGQGKHPAAMGHRGRDCWPEISP
                     SSARRSMT"
     gene            855..1082
                     /locus_tag="PKV30_12065"
     CDS             855..1082
                     /locus_tag="PKV30_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL30982.1"
                     /translation="MHQAPVAMAIMSGPRHVFTLANPLYCHILGRTEEALLGKPTARP
                     FPSWLPPGRSAGWTTPTAPTCATPAPTRSCG"
     gene            1022..1567
                     /locus_tag="PKV30_12070"
     CDS             1022..1567
                     /locus_tag="PKV30_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRL30983.1"
                     /translation="MDDAYRTDMRYASPDTLVRLNVGDGDLSERWFQFSIKPRHDLAG
                     RVQGLMSVATDVTAQVLARRALEQSHAEHQRLLKRAQDAARAKDEFLAMLGHELRNPL
                     APIVSALHVMQARQDSTTEREQAVIRRQVKHMSRLVDDFARRLAHCARPRSPAAQPDA
                     RRRHPAARGRDGATAHRPAAA"
     gene            1533..2423
                     /locus_tag="PKV30_12075"
     CDS             1533..2423
                     /locus_tag="PKV30_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL30984.1"
                     /translation="MAQPLIAQRRHELIVEPPARPVLWWGDADRLVQVVSNLLTNAAR
                     YTPVGGRIHLSGGRVAKQLVIEVTDNGMGIAPEALDGIFDVFYQVQRQASDRAAGGLV
                     LGLALVKSLVRMHEGEVEAHSDGPGQGSRFVVRLPLREQPVEAARGTLLTDAAWQHSR
                     RVLLVDDNQDGVELLAEAPALQGHQVHVAFEAEQGLLLAEQHRPQLALLDIGLPGMDG
                     HALAARMRTLLGDDCTLVALSGYSQHGDRERARAAGFAHYLVKPVDPARLLALMAAFG
                     SDAGPAQRQSPDDSAMAPLN"
     gene            complement(2614..>2798)
                     /locus_tag="PKV30_12080"
     CDS             complement(2614..>2798)
                     /locus_tag="PKV30_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRL30985.1"
                     /translation="GMDGLELTRLLRERYGDAMVLIAVSGSDPDDERVSQTFENVDHY
                     FRKPVDLAALRRLLKD"
BASE COUNT          461 a          989 c          958 g          390 t
ORIGIN      
        1 accgccacgg cctgcgcatg ggcaccagcg agatctacag cgcggtcgag ggcattgccg
       61 acgtgctcga ctcgatggtg gtcgatctcg aatacctggg ccgtgacagc tacatgccgc
      121 tgttcgtggt gctgcgcccc ggcgttgaac tggacgaggc gctgaagggc cgcatcgccg
      181 gcgccatccg ccagtcactc agcccacgct tcgtccccga cgacatcttc caggtcgccg
      241 aaatcccgcg cacgctgtcg ggcaagaaac aggagctgcc gatcaagaaa ttgctgctcg
      301 gccagccgct ggaaaaagtc gtcaaccgcg acgccatggc caacccggcg tcgctcacct
      361 ggtttgccga gttcgccgcc cagcgtgcaa gcgcttcaaa atcaggagcg gccagcgcat
      421 gacacacaag cgccaggcga ctattggaca cgaatacccg tgcttcgccg gcgacggtga
      481 agcggcccgc atgatgcgcg ccaccgactg ggccgccacg ccgctcggcc cggtggaagg
      541 ctggccgccc ggcctgggca gcatcgtcgc cacgctgctc ggctcgcgcc aaccgatgtt
      601 cctgtggtgg gggcgcgagc tggtgcagtt ctacaacgac gcctacatcc ccagccttgg
      661 ccagggcaag cacccggccg ccatgggcca tcgcgggcgc gactgctggc ccgagatcag
      721 cccatcatcg gcccgcagat cgatgacgta accggcaatt tctgcaacag ctcgccgagc
      781 agctgcgcag ttgcctggcc gaagccgaaa gccgccgcgc gcgcggcgtg gccgatgccg
      841 agcgtgacca cctgatgcac caggcgcccg tcgccatggc catcatgagc gggccgcgcc
      901 acgtgttcac gctggccaac ccgctgtact gccacatcct gggccgcacg gaagaagcgc
      961 tgctcggcaa gcctaccgcg aggcctttcc cgagctggct gccgccgggc cgcagcgccg
     1021 gctggacgac gcctaccgca ccgacatgcg ctacgccagc cccgacacgc tcgtgcggct
     1081 gaacgtgggc gacggcgacc tgtccgagcg ctggttccag ttctccatca agcctaggca
     1141 cgacctggcc ggccgcgtgc aggggctgat gtcggtcgcc accgacgtca ccgcgcaggt
     1201 gctggcgcgc cgcgcgctgg agcaaagcca cgccgagcac cagcgcctgc tgaagcgcgc
     1261 gcaggacgcc gcgcgcgcca aggacgaatt cctcgccatg ctcggccacg agctgcgcaa
     1321 cccgctggcg cccatcgtca gcgcgctgca cgtgatgcag gcgcggcagg attcgaccac
     1381 cgagcgcgaa caggccgtga tccgccgcca ggtcaagcac atgtcgcgcc tggtggacga
     1441 ttttgctcga cgtcttgcgc attgcgcgcg gccgcgttcg cctgcagccc agcctgacgc
     1501 gcgccgccgt catcctgcgg cgcgcggccg agatggcgca accgctcatc gcccagcggc
     1561 ggcatgaact gatcgtggag ccgccggcac ggccggtgct gtggtggggc gatgccgacc
     1621 ggctggtgca ggtggtcagc aacctgctga ccaacgccgc gcgctacacg ccggtcggcg
     1681 ggcgcatcca cctgtccggc gggcgcgtgg ccaagcaact ggtgatcgaa gtgaccgaca
     1741 acggcatggg catcgcgccc gaggcgctgg acggcatctt cgacgtgttc taccaggtac
     1801 agcggcaggc gtccgaccgc gcagctggcg ggctggtgct gggccttgcg ctggtcaaaa
     1861 gcctggtgcg catgcacgaa ggcgaggtcg aggcgcacag cgacggccca gggcagggca
     1921 gccgcttcgt ggtgcgcctg ccgctgcgcg agcagccggt cgaagcggcg cgcggcacgc
     1981 tgctgaccga tgccgcgtgg cagcacagcc ggcgcgtgct gctggtggac gacaaccaag
     2041 acggtgtcga actgctggcc gaggcgccgg cgctgcaggg ccaccaggtg cacgtcgcct
     2101 tcgaggccga acaagggctg ctgctggccg agcagcaccg cccgcaactg gcgctgctcg
     2161 acatcggcct gcccggcatg gacggccacg cgctggccgc gcgcatgcgc acgctgctgg
     2221 gcgacgattg cacgctggtg gcgctgtccg gctacagcca gcatggcgac cgcgaacgcg
     2281 cgcgcgccgc cggttttgcg cactacctgg tcaagcccgt cgacccggcg cgcctgctgg
     2341 cgctgatggc cgcctttggc tccgacgccg gcccagcgca gcggcaatcg ccggacgaca
     2401 gtgcgatggc gccgttgaat taagagtcaa atgggccgct ggcgcttgtg cagcaagcac
     2461 tagcagctat gaaaaagata gaaatcatgc gcggccgccg cagcaaagat ccggaggtcg
     2521 cggagcaggc caagcgggaa cgccgcggaa cgggcttggc ccggccgctg gcgttgtccc
     2581 gtgggggaag acgccgcagg cggctcaggg gggtcaatct ttcagcagcc gccgcagcgc
     2641 tgccaggtcc accggcttgc gaaagtaatg atcgacgttc tcaaacgtct ggctcacgcg
     2701 ctcgtcgtcc gggtcgctgc cgctgacggc gatcagcacc atagcgtcgc cgtagcgctc
     2761 gcgcagcagg cgcgtcagct cgaggccgtc catgccgc
//
LOCUS       DAOMVO010000314         2797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10655_length_2797_cov_13.713713, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000314 DAOMVO010000000
VERSION     DAOMVO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10655_length_2797_cov_13.713713"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="PKV30_12085"
     CDS             complement(<1..1842)
                     /locus_tag="PKV30_12085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRL30986.1"
                     /translation="MSTSPAPSSTGYSERSIKVLKGLEPVKQRPGMYTRTDNPLHVIQ
                     EVIDNAADEALAGFGKKIKVTLHTDGSVSVEDDGRGIPFGLHPEEKAPVVELVFTRLH
                     AGGKFDKGSGGAYSFSGGLHGVGVSVTNALSKRLEVSSHREGKVARIVFSGGDVVEAM
                     TLRSLETGERKQGTTVRAWPDAKYFESPALPANEMVHLLRSKAVLMPGVTVTLIDEKK
                     KDTQSWQYKGGLRDYLEQTLTADAVIPLFEGEGYAGKDSDSFAEGEGAEWAVAFTEDG
                     HPVRESYVNLIPTTAGGTHDSGLRDGLFQAVKSFIELHALLPKGVKLMPEDVFARASY
                     VLSAKVLDPQFQGQIKERLNSRDAVRLVSSYVRPALELWLNQHVDYGKRLAELAIKAA
                     QTRQRAGQKVEKRKGSGVAVLPGKLTDCESRDIADNEVFLVEGDSAGGSAKMGRDKET
                     QAVLPLRGKVLNTWEVERDRLFANTEIHDISVAIGVDPHGPGDQVDLSGLRYGKICIL
                     SDADVDGSHIQVLLLTLFFRHFPKLIEAGHLFVARPPLFRVDAPARGKKPAAKMYALD
                     EGELTAILDKLRKDGVSEGKWSIGRFKGLGEMNAEQLWETTLNPDTRR"
     gene            2009..2587
                     /locus_tag="PKV30_12090"
     CDS             2009..2587
                     /locus_tag="PKV30_12090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL30987.1"
                     /translation="MTATPHHADGTASPWVQRWTPLVAPGASVLDVACGRGRHMRWFA
                     ERGHAVTGVDRDTQALEAARAWGETVWSDIEVDPWPFEERRFGAVVVTNYLWRLLLAT
                     ILDSVQAGGVLIYETFAQGNETVGRPSRPDFLLRPGELLGLCSGPGWRVVAYEDGFLP
                     EPERFVQRIAAVRLGDGATDSPQRFPLSDARR"
     gene            2667..>2797
                     /locus_tag="PKV30_12095"
     CDS             2667..>2797
                     /locus_tag="PKV30_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRL30988.1"
                     /translation="MTPITGSIVALVTPMHEDGSVDYPTLRKLIDWHIAEGTDCIGVV
                     "
BASE COUNT          447 a          965 c          864 g          521 t
ORIGIN      
        1 gcggcgggtg tcgggattga gcgtggtttc ccacaattgt tccgcgttca tctcgcccag
       61 gcccttgaag cggccaatgg accattttcc ctcagatacg ccatccttgc gcagcttgtc
      121 gaggatggcg gtcaactcgc cttcgtccag cgcgtacatc ttggcggccg gcttcttgcc
      181 ccgcgccggc gcatcaacgc gaaacagcgg cggccgcgcc acgaacaggt gacccgcctc
      241 gatcagcttg gggaagtgac ggaagaacag cgtcagcagc agcacctgga tgtgcgagcc
      301 atcgacgtcg gcgtcggaca ggatgcagat cttgccgtag cgcagcccgc tcaaatcgac
      361 ctggtcgccc ggcccgtgcg gatcgacgcc gatggcgacc gagatgtcgt ggatctcggt
      421 gttggcgaac agccggtcgc gctcgacctc ccacgtattc agcaccttgc cgcgcagggg
      481 cagcacggct tgcgtttcct tgtcgcggcc catcttggcg ctgccaccgg ccgaatcgcc
      541 ctcgaccaga aacacctcgt tgtcggcgat gtcgcggctt tcgcaatcgg tgagcttgcc
      601 gggcagcacg gccacgcccg atcccttgcg cttttcgacc ttttgccccg cacgctggcg
      661 cgtttgcgcg gccttgatgg cgagctcggc cagccgcttg ccgtaatcga cgtgctggtt
      721 cagccagagc tccagcgccg ggcgcacgta gctggacacc aggcgcacgg cgtcgcgcga
      781 gttgagccgc tccttgatct ggccctggaa ctgcgggtca agcaccttgg cactcaagac
      841 atagctggcg cgggcaaaca cgtcctcggg catcagcttg acgcccttgg gcagcagcgc
      901 atgcaactcg atgaagctct tgacggcctg gaacagccca tcgcgcaagc cgctgtcgtg
      961 cgtgccgccc gcggtggtgg ggatcaggtt gacgtagctc tcgcgcaccg ggtggccgtc
     1021 ttcggtgaag gccaccgccc attcggcgcc ctcgccttca gcaaaactgt cggagtcctt
     1081 gccggcataa ccctcgcctt caaaaagagg gatcacggca tcggccgtca gcgtctgctc
     1141 cagatagtcg cgcagaccgc ccttgtattg ccagctttgc gtgtctttct ttttctcgtc
     1201 gatcagcgtg acggtgacgc ccggcatcaa caccgccttg ctgcgcagca ggtgcaccat
     1261 ttcgttggct ggcagcgcgg gcgattcaaa gtacttggcg tcgggccagg cgcgcaccgt
     1321 ggtgccctgc ttgcgctcgc cggtttcgag cgagcgcagc gtcatcgcct cgaccacatc
     1381 gccaccactg aacacgatgc gcgccacctt gccctcgcgg tggctgctta cctccagccg
     1441 tttgctgagc gcgttggtca ccgacacacc cacgccgtgc aggccgcccg aaaaactgta
     1501 ggcgccgccc gagcccttgt cgaacttgcc gcccgcgtgc agccgcgtga acaccagctc
     1561 gaccaccggc gctttctctt ccggatgcag gccgaacgga atgccgcgtc catcatcctc
     1621 cacgctcact gagccatcgg tgtgcagcgt gaccttgatc ttcttgccga acccggccag
     1681 cgcctcgtcg gccgcgttgt cgatcacctc ctggatcacg tgcagcgggt tgtcggtgcg
     1741 cgtgtacatg cccggccgct gcttgaccgg ctccagccct ttcaggacct tgatggaacg
     1801 ctcggaatag ccagtagagg acggtgcggg tgacgtggac ataaccgcgc attttagccc
     1861 gttggcgcga tttcactgga tgcaaacaca gatggcgcgc cagacgcgtg cccctcaact
     1921 ctctccaatc aggttaaaaa atgcctccag cgcttgatgg acaagcgctg gcagctatca
     1981 atagcatagt tgcgctatgc tttgcgccat gaccgccacg ccccaccacg ccgacggcac
     2041 cgcttcgccc tgggtccagc gctggacgcc gctggttgcg ccgggcgcgt cggtgctcga
     2101 cgtcgcctgc gggcgcggcc ggcacatgcg ctggttcgcc gaacgcggtc acgccgtcac
     2161 cggcgtcgac cgcgacaccc aagcgctcga ggctgcgcgc gcgtggggcg aaaccgtttg
     2221 gtccgacatc gaggtcgatc cctggccttt cgaggagcgg cgttttggcg ctgtcgtcgt
     2281 caccaactac ctgtggcgct tgctgttggc gacgatcctg gacagcgtcc aggcaggcgg
     2341 cgtgctgatc tacgaaacct tcgcccaagg caacgaaacc gtgggccgcc cgtcgcgccc
     2401 cgacttcctg ctgcggccgg gcgaactgct gggcctgtgc agcgggccgg gctggcgcgt
     2461 ggtggcgtac gaagacggct tcttgcccga gcccgagcgc ttcgtgcagc gcatcgccgc
     2521 ggtgcggctg ggcgacgggg cgaccgattc gccccaacgg tttccgctgt cagacgcgcg
     2581 ccgatagcgt ggcggctgcg gtgtgaacct tttgtcgcac acccatctaa aatgcacgtt
     2641 ttacgcccga aactccctcg gattccatga cccccatcac aggcagcatc gtcgccctcg
     2701 tcacccccat gcacgaggac ggcagcgtcg actacccgac gctgcgcaag ctgatcgact
     2761 ggcacatcgc cgaaggcacc gactgcatcg gcgtggt
//
LOCUS       DAOMVO010000315         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10800_length_2768_cov_17.725765, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000315 DAOMVO010000000
VERSION     DAOMVO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10800_length_2768_cov_17.725765"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..1938
                     /locus_tag="PKV30_12100"
     CDS             421..1938
                     /locus_tag="PKV30_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HRL30989.1"
                     /translation="MQMLVSAGLIALVVVWGLLAPASLGAVFNEALAAITRNFGWFYL
                     WVVLGLVLLALVLAFSRYGDMKLGADDDEPEFSNGAWFSMLFAAGMGIGLVYFGVAEP
                     LSHFASPPPGVAARTPEAANAAMRYAFFHWGLHPWAIYGIVGLAIAYFQFRRGAPALV
                     SSATETLPWAWARRLTPAFNVLAVVATAFGVAASLGVGAAQINSGLHTVFGLPVGPTW
                     QVGIIAVVTVLFVTSSVSGVARGVKLLSVGNLIAAAALAGVVFVLGPTVHIIDTFTNS
                     LGGYASEFVRMSLRLTPFRDSSWVGSWTIFYWAWWLAWSPFVGLFIARVSRGRTIREF
                     VLGVVIAPTLVAFLWFSIFGGAALSLEIFQGAPLADVAAKDSSLALFAMLQQLPLATL
                     TSIIGTLLVVVFFVTSGDSATLVLATMSHGGADNPPHRSKIIWGVLIAGIAAALLLAG
                     GVQAVQTASIVFALPFTVVVVLMAVALVRAIRADWEADQKRQRALRRRMEQLVQP"
     gene            2064..2483
                     /locus_tag="PKV30_12105"
     CDS             2064..2483
                     /locus_tag="PKV30_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRL30990.1"
                     /translation="MNGRRILTALAAVLLGALAWRTGGWAGLALLGSGLLLWLLLNYT
                     RIMTIMKRAADRPVGYVGSAVMLNARLKQGMPLLDVLALTRALGERQSPPEAEPDVYR
                     WRDAGESVVTAEFEGGKLARWQLVRPAEPPAEAPPAA"
     gene            2540..>2768
                     /locus_tag="PKV30_12110"
     CDS             2540..>2768
                     /locus_tag="PKV30_12110"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481177.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HRL30991.1"
                     /translation="MSTPALSMADRDGQIWFDGQLVDWRDAKIHVLTHTLHYGCGAFE
                     GVRAYNTAGGTAIFRLQEHTQRLFNSAKILRM"
BASE COUNT          368 a          915 c          963 g          522 t
ORIGIN      
        1 cacgccgtgg agcgcgtgga tgaggcgatg gcgctggtgc gcgggctgga ttgaagcttt
       61 attattgata gctgcttgcg cttgatggac aagcgccagc ggccattttc gatctgtttt
      121 ttggatcgcc catccgcgtt ccacatcatg acaacccagt ttgcgacaat cgcttacttt
      181 cgcggcgcat gcagtgcgtt gccagcggat cggcgccaga tcgcatccgt tggcgcctga
      241 tgcgccttct cggcccccgc gcctggcggg cgccacctgt tcatccccct gatcgcccgg
      301 cgctccagtg cgccttgccg ccccttgggg cggcgctaat ggcgcaacgg ggcgcatgct
      361 gcatttgcgg caagccttca agcttgccga ccgcacgtgc caaagaagaa ggaaaaaccc
      421 atgcaaatgc tggtttcagc gggcctgatc gccctcgtcg tcgtctgggg gctgctggca
      481 cccgcctcgc tgggcgcggt gttcaacgag gcgctggccg ccatcacgcg caacttcggc
      541 tggttctacc tgtgggtggt gctgggcctg gtgctgctgg cgctggtgct ggccttcagc
      601 cgctatggcg acatgaagct gggcgccgac gacgacgagc ccgagttcag caacggcgcc
      661 tggttctcga tgctgtttgc ggccggcatg ggcatcgggc tggtgtactt tggcgttgcc
      721 gagccgctgt cgcactttgc gtcgccgccg cccggcgtgg ccgcgcgcac gcccgaggcg
      781 gccaacgccg ccatgcgcta cgccttcttc cactgggggc tgcacccgtg ggccatctac
      841 ggcatcgtcg ggctggcgat tgcgtatttc cagtttcggc gcggcgcgcc ggcgctggtc
      901 agttcggcca ccgagacact gccctgggcc tgggcgcggc gcctgacgcc ggccttcaac
      961 gtgctggcgg tggtggccac cgcgtttggc gtggcggcgt cgctgggcgt tggcgcggcg
     1021 cagatcaaca gcgggctgca caccgtgttc gggctgccgg tcgggccgac gtggcaggtt
     1081 ggcatcatcg ccgtggtgac ggtgctgttc gtcacttcgt ccgtcagcgg cgtggcgcgc
     1141 ggcgtcaagc tgctgtcggt cggcaacctg atcgcggccg cggcgctggc gggggtggtg
     1201 ttcgtactgg ggccgacggt gcacatcatc gacaccttca ccaattcgct gggcggctac
     1261 gccagcgagt ttgtgcgcat gagcctgcgg ctgacgccgt tccgcgattc aagctgggtc
     1321 ggcagctgga ccatttttta ctgggcctgg tggctggcgt ggtcgccttt cgtcggactg
     1381 ttcatcgccc gcgtgtcgcg cgggcgcacg atccgcgaat tcgtgctggg cgtggtgatc
     1441 gcgccgacgc tggtggcgtt tttgtggttc tcgatcttcg gcggcgccgc gctgagcctg
     1501 gagattttcc agggcgcgcc gctggccgac gtggcggcca aggattcgtc gctggcgctg
     1561 tttgccatgc tgcagcagtt gccgctggcc acgctgacct cgatcatcgg cacgctgctg
     1621 gtggtggtgt tcttcgtcac ctcgggcgat tcggccacgc tggtgctggc caccatgagc
     1681 cacggcggcg ccgacaaccc gccgcaccgc agcaagatca tctggggcgt gctgatcgcc
     1741 ggcattgccg ccgcgctgct gctggccggc ggcgtgcagg cggtgcagac ggcctcgatc
     1801 gtgtttgcgc tgccgttcac ggtggtggtg gtgctgatgg cggtggcgct ggtgcgtgcc
     1861 atccgcgccg actgggaggc tgatcagaag cgccagcgcg cgctgcgccg gcgcatggag
     1921 cagctggtgc agccctgatt cgcgcaacct cgattgctat tgcttgcata gctgctcgcg
     1981 cttgcttgac aagcgccaga ggcatttttg accacaaatc tccgaccgaa cccgtgcccg
     2041 ttgccaggcg cgacaatcaa gccatgaacg gacgacgaat cctgaccgcc ctggccgccg
     2101 tgctgctggg tgccctggcc tggcgcaccg gcggctgggc cggcctggcg ctgctgggca
     2161 gcgggctgct gctgtggctg ctgctcaact acacgcgcat catgaccatc atgaagcgcg
     2221 ccgccgaccg acccgtgggc tacgtgggca gcgcggtgat gctgaacgcc cggctgaaac
     2281 agggcatgcc gctgctcgac gtgctggcgc tcacgcgcgc gctgggcgag cgccagtcgc
     2341 cgcccgaggc cgagcccgac gtctaccgct ggcgcgacgc cggtgaatcg gtggtgacgg
     2401 ccgaattcga aggcggcaag ctggcgcgct ggcaactggt gcggcccgcc gagccgccgg
     2461 ccgaggcgcc gccagcggct taaaatcagc cggtttgacg gccggcccgt cgagttcacc
     2521 gcctgcacaa ggaacaccca tgagcacccc cgccctttcc atggccgacc gcgatggcca
     2581 gatctggttc gacggccagc ttgtcgactg gcgcgacgcc aagatccacg tgctgacgca
     2641 cacgctgcac tacggctgcg gcgcgttcga gggcgtgcgg gcctacaaca cggcgggcgg
     2701 caccgccatc ttccgcctgc aggaacacac ccagcgcctg ttcaacagcg ccaagatcct
     2761 gcgcatga
//
LOCUS       DAOMVO010000316         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10839_length_2761_cov_20.098300, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000316 DAOMVO010000000
VERSION     DAOMVO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10839_length_2761_cov_20.098300"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2761)
                     /locus_tag="PKV30_12115"
     CDS             complement(<1..>2761)
                     /locus_tag="PKV30_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL30992.1"
                     /translation="MVVRTNWPGATAQQVAEQVTDKIERTLQEVPYADKIRSYSKPGE
                     SMIIFQLKDNSPPKEVPQIWYTVRKKVGDMRYTLPQGVQGPFFNDDFGDVYGVIYTLS
                     GDGFSYAELKQFADDARQQLLRVKDVAKVELFGVQDEKIYIEISQKRLAELGLDMNQV
                     LGQLGAQNAIESAGAVQTPLDVVQVRVGGQFNDVEQLRAMPIRGASGNQLRLGDIAEI
                     ERGLIDPPQVKVRHDGHEVIALGVSMAKGGDIIALGKSLKDVSAKIEKSLPLGVALQQ
                     VQDQPKAVADSVSEFVKVLIEAVVIVLAVSFLSLGLHKRQGRHPFWRRWTLDVRPGLV
                     VGITIPLVLAVTFLAMQYWGIGLHKISLGSLIIALGLLVDDAIIAVEMMVRKMEEGYD
                     KFRAATFAYEITAIPMLTGTLITAVGFLPIGIAKSMTGEYTFAIFAVTVIALLVSWVA
                     SVVFVPLLGAWLLRVPPHVAAARAEALNSGQPAHDEPHEMFDSAFYNGFRRLVNWCVA
                     HRWLTIAATVALFALGVLGMGKVQQQFFPDSSRPEILVDLWSPEGTSFTANEAVAKRV
                     EARLMAEPGVTSVTQWIGAGVPRFYLPLDQIFPQTNVSQLIVLPRDLATRDQLRLKLP
                     TILAAEFPEVRGRVKLLPNGPPVPYPVQFRVIGPDPQRLRAHADEVTAIMRDSANTRG
                     VNDNWNESIKAIRLEVDQAKARALGVTSQSIAQAGRVMLAGSTVGQYREGDRLIDIVL
                     RQPLDERDAITDIANAYLPTASGKSIPLTQVAKPVFVWEPGVMWRENRDYAITVNADV
                     VEGLQGATVTNELLPPLRALESKWAQAGEGAYRIEVAGAVEESSKGSASIAAGVPVML
                     FVIFTLLMLQLHSFSRSMLVFLTGPLGIAGVAAALLVLNRPFGFVALLGVIALMGMIQ
                     RNSVIL"
BASE COUNT          475 a          966 c          852 g          468 t
ORIGIN      
        1 caggatcacc gagttgcgct ggatcatgcc catcagcgcg atcacgccca gcagcgcgac
       61 aaagccgaac ggccggttca gcaccagcag cgccgccgcc acgccggcaa tgccgagcgg
      121 cccggtcaag aacaccagca tggatcggct gaagctgtgc aactgcagca tcagcagcgt
      181 gaagatgacg aacagcatga ccggcacgcc agcggcgatc gaggccgagc ccttgctgct
      241 ttcttccacc gcgccagcga cctcgatgcg gtaggcgccc tcgcccgcct gcgcccactt
      301 ggattcgagc gcgcgcagcg gcggcagcaa ctcgttggtc acggtggcgc cctgcaggcc
      361 ttccaccacg tcggcgttga cggtgatggc gtagtcgcgg ttttcgcgcc acatcacgcc
      421 cggttcccac acgaacaccg gcttggccac ctgcgtgagc ggaatcgact tgccgctggc
      481 cgtcggcagg taggcattgg cgatgtcggt gatggcgtcg cgctcgtcca gcggctgccg
      541 cagcacgatg tcgatcagcc tgtcgccctc gcggtactgc cccaccgtgc tgccggccag
      601 catcacgcgt ccggcctggg cgatggactg gctggtcacg cccagcgcgc gtgccttggc
      661 ctgatcgacc tccagccgga tggctttgat ggattcgttc cagttgtcgt tgacgccgcg
      721 cgtgttggcg ctgtcgcgca tgatggccgt cacctcgtcg gcgtgcgcgc gcaggcgctg
      781 cgggtcgggg ccgatgacgc ggaattgcac cgggtagggc accggcggcc cgttgggcag
      841 cagcttgacg cggccgcgca cctcggggaa ttcggccgcc agaatggtcg gcagcttgag
      901 ccgcagctgg tcgcgcgtgg ccagatccct gggcagcacg atcagttgcg acacgttggt
      961 ctgcggaaaa atctgatcca gcggcagata gaagcgcggc acgccggcgc cgatccactg
     1021 cgttacgctg gtcacgcccg gctcggccat caggcgcgcc tcgacccgct tggccaccgc
     1081 ttcgttggcg gtgaaagacg tgccctcggg cgaccacaga tcgaccagaa tctctggccg
     1141 gctcgaatcg gggaagaact gctgctgcac cttgcccatg cccagcacgc caagcgcaaa
     1201 caacgccacc gtggcggcaa tcgtcagcca gcggtgcgcc acgcaccagt tcaccaggcg
     1261 tcgaaagccg ttgtagaacg cgctgtcgaa catctcgtgc ggttcgtcgt gcgccggctg
     1321 gccgctgttg agcgcttcgg cgcgcgcggc ggccacgtgc ggcggcacgc gcagcagcca
     1381 ggcgcccaag agcggcacga acaccaccga cgccacccag ctcaccagca gcgcaattac
     1441 cgtcaccgcg aagatggcaa aggtgtattc gcccgtcatc gacttggcga tgccgatggg
     1501 cagaaaaccc accgccgtga tcagcgtgcc ggtcagcatg gggatggccg tgatctcgta
     1561 cgcaaaggtg gcggcgcgga acttgtcgta gccctcttcc atcttgcgca ccatcatttc
     1621 gacggcaatg atggcgtcgt ccaccagcag gcccagcgcg atgatcagcg agccgagcga
     1681 gatcttgtgc agcccgatgc cccagtactg catggcgaga aaagtcaccg ccagcaccag
     1741 cggaatggtg atgcccacca ccaggcccgg gcgcacatcc agcgtccagc ggcgccagaa
     1801 cggatgccgc ccctggcgct tgtgcagccc caggctcaaa aagctcacgg ccagcacgat
     1861 caccaccgcc tcgatcagca ccttgacgaa ttcgctcacc gaatcggcca ccgccttggg
     1921 ctggtcctgc acctgctgca acgccacgcc gagcggcagg cttttttcga tcttcgcgct
     1981 cacgtctttc agcgatttgc cgagcgcaat gatgtcgccg cccttggcca tcgacacacc
     2041 cagcgcaatc acctcgtggc cgtcgtggcg caccttgacc tgcggcggat cgatcaagcc
     2101 gcgctcgatc tccgcgatgt cgcccagacg cagctggttg cccgaagcac cccggatcgg
     2161 catggcacgc aactgctcga cgtcgttgaa ctggccgccc acgcgcacct gcaccacgtc
     2221 cagcggcgtc tgtaccgcgc cggccgattc aatggcgttt tgcgcgccca gctggcccag
     2281 cacctggttc atgtccagcc ccagctcggc caagcgcttt tgcgagattt cgatgtagat
     2341 cttctcgtcc tgcacgccga acagctcgac cttggccacg tccttcacgc gcagcagttg
     2401 ctggcgcgcg tcgtcggcaa actgcttcaa ctcggcatac gaaaaaccat cgcccgacag
     2461 cgtgtagatc acgccgtaca cgtcgccgaa atcgtcgttg aagaacggcc cctgcacgcc
     2521 ctgcggcaag gtgtagcgca tgtcacccac cttcttgcgc accgtgtacc agatctgcgg
     2581 cacctccttg ggcggcgagt tgtctttcag ctgaaagatg atcatcgact cgcccggctt
     2641 gctgtagctg cggatcttgt cggcgtaggg cacttcctgc agcgtgcgct cgatcttgtc
     2701 ggtgacctgc tcggccacct gctgcgccgt ggcgccgggc cagttggtgc gcaccaccat
     2761 g
//
LOCUS       DAOMVO010000317         2758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10860_length_2758_cov_8.967074, whole genome shotgun sequence.
ACCESSION   DAOMVO010000317 DAOMVO010000000
VERSION     DAOMVO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10860_length_2758_cov_8.967074"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKV30_12120"
     CDS             complement(<1..205)
                     /locus_tag="PKV30_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase (NADP(+))"
                     /protein_id="HRL30993.1"
                     /translation="MDAKATPLALLKDPTLLKTDGLIDGKWVKGKARFDVIDPATGAK
                     LADVADLGPRETKAAIAAANQAWP"
     gene            complement(342..1802)
                     /gene="cls"
                     /locus_tag="PKV30_12125"
     CDS             complement(342..1802)
                     /gene="cls"
                     /locus_tag="PKV30_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRL30994.1"
                     /translation="MIAWLQQFILAGHPLTVWIGWTWTISSIVLTTWIILQRKSPVST
                     LAWIMVLNLMPVVGLLVYAYFGPQRIKRQRLKRWHKRAALMSREDVSALRAERPDVPA
                     WAVQHARLIEEASGLPMSSAQSVDVLASGGATLDALLREIDGARGHIHLEYYIFEPDE
                     TGSRLLQALIAKARAGVKVRLLVDAIGSPKLLSRRHRRLLDEFRAAGGEFAVFHPARL
                     DRLRPLVNLRTHRKIVVIDGHTGFVGGINVTDDENEHIRPDHAYRDTHLLIRGGAVRW
                     LQYVFLRDWHYAEGRPPAPDEDLLPEQAPGTLPVQIVTSGPDSDTEAIHRAMIDALNM
                     ARERIWLATPYFVPTEAAYTALTNAALRGVQVKLMVPEKSDSRVVTAAARSYFKELQD
                     AGVLVFEYRGRMFHAKTLLVDELYGMVGSANFDNRSFRLNFEVMAVVIERDFNQRLAE
                     MFDDDLSHCKLVPLDRRAPPWQQLFEAVARLASPLL"
     gene            complement(1816..2172)
                     /locus_tag="PKV30_12130"
     CDS             complement(1816..2172)
                     /locus_tag="PKV30_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HRL30995.1"
                     /translation="MASITLYGIPNCDTVKKARAWLAERGVDHAFHDFKKQGVPPEHL
                     ARWLAAAGWDKVLNRQGTTWRKLDAAAQAAVHDAASAADLLLREPSAIKRPVVEWANG
                     AITVGFAPDAWSQRLG"
     gene            2214..>2758
                     /locus_tag="PKV30_12135"
     CDS             2214..>2758
                     /locus_tag="PKV30_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRL30996.1"
                     /translation="MKTLDDWLAHCEQLHPKTIDMGLDRVRAVAERMGLRFDCPVFTV
                     AGTNGKGSTCAMLEAILTQAGFRTGLFTSPHLVRFEERCRIRGQNVDASDLVAAFARV
                     ESARAEISLTYFEFTTLAILDTLARAGLDAVILEVGLGGRLDAVNIIDTDCAIITSID
                     IDHAELLGDTREKIGFEKAGIL"
BASE COUNT          463 a          897 c          928 g          470 t
ORIGIN      
        1 cgggccaagc ttggttggcg gcggcgatgg cggccttggt ctcgcgcggg cccagatcgg
       61 ccacgtcggc cagcttggcg ccggtggcgg ggtcaatgac gtcaaagcgt gccttgccct
      121 tgacccactt gccatcgatc aggccatcgg ttttcagcag ggtagggtct ttcagcaggg
      181 caagcggggt ggcttttgcg tccatggtgt ctccaggcat tgggttcgtg atacgagcca
      241 ttgtaggcaa ggcgtgtgac ccctcgcttc aagggtttta aggcgccaga gcttgcccat
      301 caagcgcgag cagctacgta aaagatagct taagggttac gctacaagag cggcgacgcc
      361 aaccgcgcca ccgcctcaaa caactgctgc cacggcggcg cgcgccgatc cagcggcacc
      421 agcttgcagt gcgacaaatc gtcgtcgaac atctcggcca gccgctggtt gaagtcgcgc
      481 tcgatcacca ccgccatcac ctcgaagttg aggcggaagc tgcggttgtc gaagttggcg
      541 ctgcccacca tgccgtacag ctcgtccacc agcagcgtct tggcgtggaa catgcggccg
      601 cggtattcga acaccagcac gccagcgtcc tgcaactcct tgaagtacga gcgcgcggcg
      661 gccgtcacca cgcgcgaatc gctcttctcg ggcaccatca gcttgacctg cacgccgcgc
      721 agtgcggcgt tggtgagtgc ggtgtaggcc gcttcggtcg gcacgaagta aggcgtggcc
      781 agccagatgc gttcgcgcgc catattcagc gcgtcgatca tggcgcggtg aatcgcctcg
      841 gtgtccgaat cggggccgct ggtgacgatt tgcaccggca gcgtgccggg cgcctgctcg
      901 ggcagcaggt cttcgtcggg cgccggcggg cggccttcgg cgtagtgcca gtcgcgcaaa
      961 aacacgtatt gcagccagcg caccgcgccg ccgcgaatca gcaaatgcgt gtcgcggtag
     1021 gcgtggtcgg ggcggatgtg ctcgttctcg tcgtcggtca cgttgatgcc gccgacaaag
     1081 ccggtgtggc cgtcgatcac cacgattttg cggtgcgtgc gcaggttgac cagcggccgc
     1141 aggcgatcga gccgcgcggg gtggaacacc gcaaattcgc cgcccgccgc gcgaaactca
     1201 tccagcaacc gccgatgccg gcgcgacagc agcttgggcg agccgatggc gtccaccagc
     1261 aggcgcacct tcacgccggc gcgcgccttg gcgatcagcg cctgcagcag acgcgagccg
     1321 gtctcgtcag gctcgaagat gtagtactcg aggtggatgt gcccgcgcgc gccgtcgatc
     1381 tcgcgcagca gcgcatccag cgtggcgccg ccgctggcca gcacgtcgac gctttgcgcg
     1441 ctcgacatcg gcaggccgct ggcttcttcg atcagtcgcg cgtgctggac cgcccaggcg
     1501 ggcacgtcgg ggcgctcggc gcgcagcgcg ctcacgtcct cgcgcgacat cagcgcggcg
     1561 cgcttgtgcc agcgcttgag ccgctggcgc ttgatgcgct gcgggccaaa gtaggcgtac
     1621 accagcaagc ccaccaccgg catcaggttc aacaccatga tccaggccag cgtcgacacc
     1681 ggcgacttgc gctgcaggat gatccaggtg gtgaggacga tgctggaaat cgtccacgtc
     1741 cagccgatcc acaccgtcag cggatgcccg gcaaggatga attgctgtag ccaggcgatc
     1801 atctgggaag cggcttcagc cgagccgctg tgaccaggcg tccggcgcaa agccgacggt
     1861 gatggcgccg ttggcccatt cgaccaccgg gcgcttgatg gcgctgggct cgcgcagcag
     1921 caaatcggcg gcgctggcgg cgtcgtgcac ggcggcttgc gcagccgcat ccagcttgcg
     1981 ccaggtcgtg ccctggcggt tgagcacctt gtcccagccc gccgcggcca gccagcgcgc
     2041 caggtgctcg ggcggcacgc cctgcttctt gaaatcgtga aaggcgtggt ccacgccgcg
     2101 ctcggccagc caggcgcgcg ccttctttac ggtgtcgcag ttggggatgc cgtacagggt
     2161 gatgctcgcc atggcgggcg atgatgccac gggtgaaaga caatcggcgc ctgatgaaaa
     2221 cgctcgacga ctggctcgcg cactgcgagc aactgcaccc gaaaaccatc gacatggggc
     2281 tggaccgcgt gcgtgccgtg gccgagcgca tggggttgcg ctttgactgc ccggtgttta
     2341 ccgtcgccgg caccaacggc aagggatcga cctgcgccat gctggaggcg atcctgacgc
     2401 aagcgggttt tcgcaccggg ctgttcacct cgccgcacct ggtgcggttt gaagaacgct
     2461 gccggattcg cggccagaac gtcgatgctt ctgatttggt agctgccttc gcccgtgtgg
     2521 aaagcgccag ggccgaaata tccttgacgt atttcgagtt caccacgctc gccatcctcg
     2581 acacgctggc gcgcgcgggg ctcgacgcgg tgatcctgga agtgggcctg ggcggtcgac
     2641 tggacgcggt gaacatcatc gacaccgact gcgccatcat caccagtatc gacatcgacc
     2701 acgccgaact gctgggcgat acgcgcgaga agatcggctt cgagaaagcc ggcatcct
//
LOCUS       DAOMVO010000318         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10871_length_2756_cov_11.863384, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000318 DAOMVO010000000
VERSION     DAOMVO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_2756_cov_11.863384"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKV30_12140"
     CDS             <1..576
                     /locus_tag="PKV30_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRL30997.1"
                     /translation="LKERDAGEGVHHSRMWHAALDAIQYGPVPVVAALHGAVVGGGLE
                     LASAAHIRVADASTFYALPEGTRGIFVGGGGSVRIPKLIGTARMMDMMLTGRVYNAED
                     GERIGLAQYRVAEGSAFDKALELATRIATNAPLTNYALTHALPRIAEQPADHGYMTEA
                     LISAIAQAAPEAKQRVRDFLEGKAGTKVKKA"
     gene            648..2537
                     /locus_tag="PKV30_12145"
     CDS             648..2537
                     /locus_tag="PKV30_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HRL30998.1"
                     /translation="MSATETSPRYRPLAFGVTRVEVRAGDDGAQYLAADVPLQDFDTR
                     MSDRLIHWARTTPDETFIAQRERLADGQLGDWRRVSYAQALDAARRIGQALLTRGLSA
                     ERPVVILSDNGIDHALLALGCLYAGVPYSPASPAYSTISKDYGKLRHVIDTLTPGLIW
                     AEDGARYGAAIAAVASGGCEVVLSRNADHTSLGGRASTPLAELLATEATPAVDAAQQA
                     TTPDTIVKFLFTSGSTKLPKAVINTHRMWCANQQQMRQSMPLLTEERPVLIDWLPWNH
                     TFGGNHNFGMVLANGGTLYIDEGKPTPALIHETLRNLREIAPTMYFNVPTGFEAIAQT
                     MKTDDALRRNFLSRVKMFFYAGASLAQPVWDSLFESAERELGERIVMTCGFGMTESSP
                     YGLFVTSPDVKAGDLGLPTPGMDVKLAPSNGKLEIRYRGPNITPGYWRAPEATAEAFD
                     DEGFFCTGDAVTWCDATNPHAGLRFDGRIAEDFKLATGTFVSVGPLRGRVIAGGAPYI
                     QDVVLTGLDRKEVGALIFTTPAVRSLTDLPSDAPLAQVLADRRVLQKFQSLATDLSLS
                     ATGSANRVTRLMLMAEPPSIDLGEVTDKGSINQRAVLKHRDALVQALYDETPGHIVIK
                     PGVNL"
     gene            2552..>2756
                     /locus_tag="PKV30_12150"
     CDS             2552..>2756
                     /locus_tag="PKV30_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRL30999.1"
                     /translation="MAAKGFFTAFDDVWMLDGVRTPMVDYCGAFGHISPTDLGIKAAR
                     EALKRAGVPGSDIGSVVTGNMAPG"
BASE COUNT          476 a          970 c          897 g          413 t
ORIGIN      
        1 ctgaaagagc gcgacgccgg cgaaggcgtg caccactcgc gcatgtggca tgcggcgctc
       61 gatgcgatcc agtacggccc ggtgcccgtg gtcgccgcgc tgcacggcgc ggtggtcggc
      121 ggcgggctgg agttggccag tgccgcgcac atccgcgtgg ccgatgcctc cactttttac
      181 gcgctgcccg aaggcacgcg gggcatcttc gttggcggcg gcggctcggt gcgcatcccc
      241 aagctgatcg gcaccgcgcg catgatggac atgatgctca ccggccgcgt ctacaacgcc
      301 gaagacggcg agcgcatcgg cctggcgcag taccgcgtgg ccgaaggcag cgcgttcgac
      361 aaagcactgg agctggccac ccgcatcgcc accaacgcgc cgctcaccaa ttacgcgctg
      421 acgcacgccc tgccccgcat cgccgagcag ccggccgacc acggctacat gaccgaggcg
      481 ctgatcagcg ccatcgcgca agcggcgcca gaagccaagc agcgcgtgcg cgatttcctc
      541 gagggcaagg cggggacgaa agtcaagaag gcttgaagcc ttttaggccc gcagcgcttg
      601 tctatcaagc gcaagcagct ataaaaatca gagtatccga gacaagcatg agcgccaccg
      661 agacctcccc ccgctaccgc ccgctggcct tcggcgtcac ccgcgtcgaa gtgcgcgcgg
      721 gcgatgacgg cgcgcaatac ctggcggccg atgtgccgct gcaggatttc gacacgcgca
      781 tgtcggatcg cctgatccat tgggcgcgga cgaccccgga cgaaaccttc atcgcccagc
      841 gcgagcggct ggccgacggc caactgggtg actggcgccg cgtgagctac gcccaggcgc
      901 tggatgccgc gcggcgcatt ggtcaggcgc tgctcacgcg cgggctgtcg gccgagcggc
      961 cggtggtgat cctgtccgac aacggcatcg atcacgcgct gctggcgctc ggctgcctgt
     1021 acgccggcgt gccgtattcg cccgcctcgc ccgcgtattc gaccatcagc aaggactacg
     1081 gcaagctgcg ccacgtcatc gacacgctga cgcccgggct gatctgggcc gaggacggcg
     1141 cgcgctatgg cgcggcgatt gccgccgtgg cctccggcgg ctgcgaagtc gtgctgtcgc
     1201 gcaacgccga tcacacatcg cttggcggcc gtgccagcac gccgctggcc gaactgctgg
     1261 ccaccgaagc cacgcccgcc gtcgatgccg cccagcaagc caccacgccc gacaccatcg
     1321 tcaagttcct gttcaccagc ggctcgacca aattgcccaa ggccgtcatc aacacccacc
     1381 gcatgtggtg cgccaaccag cagcagatgc gccagtcgat gccgctgctc accgaagagc
     1441 gcccggtgct gatcgactgg ttgccctgga accacacctt cggcggtaac cacaacttcg
     1501 gcatggtgct ggccaatggc ggcacgctct acatcgacga aggcaagccg acgcccgcgc
     1561 tgatccacga gacgctgcgc aacctgcgcg agatcgcgcc aacgatgtac ttcaacgtgc
     1621 cgacgggctt cgaggccatt gcgcagacca tgaagacgga cgatgcgctg cgccgcaact
     1681 tcctctcgcg cgtcaagatg ttcttctacg ccggtgcgtc gttggcgcag ccggtgtggg
     1741 actctctttt cgagagcgcc gagcgcgaac tgggcgagcg cattgtcatg acctgcggct
     1801 tcggcatgac cgaatccagc ccttatggcc tgttcgtcac cagccccgac gtgaaggccg
     1861 gcgatctcgg cctgccgacg ccgggcatgg acgtgaagct ggcgcccagc aacggcaagc
     1921 tcgagatccg ctaccgcggc cccaacatca cgcccggcta ctggcgcgcg cccgaggcca
     1981 cggccgaagc gttcgacgac gaaggctttt tctgcaccgg tgacgccgtg acctggtgcg
     2041 acgcgacgaa cccgcacgcc ggcctgcgct tcgacgggcg catcgcggaa gacttcaagc
     2101 ttgctaccgg cacctttgtc agcgtcgggc cgttgcgcgg gcgcgtgatt gcgggcggcg
     2161 cgccgtacat ccaggacgtg gtgctgacgg ggctggaccg caaggaagtt ggcgccctga
     2221 tctttaccac gccggccgtg cgcagcctga ccgatctgcc gtccgatgcc cccctggcgc
     2281 aggtgctggc tgatcggcgc gtgctgcaga aattccagag cctggccacc gacctgtcgc
     2341 tcagcgccac cggcagcgcc aaccgcgtga cgcggctgat gctgatggcc gagccgcctt
     2401 cgatcgacct gggcgaagtg acggacaagg gctcgatcaa ccagcgcgcc gtgctcaagc
     2461 accgcgatgc gctggtgcag gcgctgtacg acgagacacc ggggcacatc gtcatcaagc
     2521 ccggtgtgaa cctctgactc aaggacaaga catggctgcc aaaggattct tcaccgcctt
     2581 cgacgacgtc tggatgctcg atggcgtgcg cacgcccatg gtcgactact gcggcgcgtt
     2641 cggccacatc agccccaccg acttagggat caaggcggcg cgcgaggcgc tcaagcgcgc
     2701 cggcgtgccg ggcagcgaca tcggctcggt cgtcaccggc aacatggcgc cgggcg
//
LOCUS       DAOMVO010000319         2749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10905_length_2749_cov_11.666667, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000319 DAOMVO010000000
VERSION     DAOMVO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10905_length_2749_cov_11.666667"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKV30_12155"
     CDS             <1..706
                     /locus_tag="PKV30_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRL31000.1"
                     /translation="LRRLAGIATRVIHLAPPPAEGEGDPRSAALLRALARRTSPLALV
                     YASTSGVYGDCGGAWIDETRALRPQTARARRRVAAESALRAFGRAAGTRTSILRIPGI
                     YAPDREGGTPRARLERGTPVLAADDDVYTNHIHADDLARAVAAALWRGKPQRAVNASD
                     DTELKMGDYFDLAADLYGLPRPPRIGRDQAREQLAPTLLSFMSESRRLLNGRLKRELR
                     LRLDHATPATGLLSKR"
     gene            830..1804
                     /locus_tag="PKV30_12160"
     CDS             830..1804
                     /locus_tag="PKV30_12160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL31001.1"
                     /translation="MTLIDKPRADRRAFITGSAAWAASGLLASGGLQAQQLRAGSFAA
                     GDYKPRRGQSGKDVIWIPTPDLFVTRMLRLAQVTPQDFVIDLGSGDGKIVIAAAREGA
                     RGLGIEYNPDMVELSRRRAQEAGVAERTRFEKADIFESDFSQATVVTMYLLPQLNLKL
                     RPRILALKPGTRVVSHEFRMGRWRPDETSNVDHAALHLWLVPANVGGQWELQFPQQAG
                     PVTTRLEVRRQHFQDFTGTVGFKDFDTGLREPKVAGEQVRFGFTDEDGRLRRFVGQVA
                     GDVINGSVYDLTAGGARAPFSARRIGAAPAIDGSGPAAADEAESLGND"
     gene            1804..>2749
                     /locus_tag="PKV30_12165"
     CDS             1804..>2749
                     /locus_tag="PKV30_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRL31002.1"
                     /translation="MQPGSETTQPQQTLRLREAVAIIVGIVIGAGIFKAPSLVAQFTG
                     SIGWMLAAWVAGGVISLIGALCYAELAAAWPHAGGDYHFLQRAYGRRVAFLFAWARFS
                     VITTGSIALLAFVFGDYMSAVLPLGTYSAALWGALAVVVLTWLNARGIHASASAQSWL
                     TLAEVLGLVLVIAAGLWMWSSSVHRMPMPDGAVVAGAVSAMPGLGMLGLAMVFVLLTY
                     GGWNEAAYLSAELRGSRRGMLHALVISLALITVLYLLVNGAYVYGLGIQGMAKSEAVA
                     ADLLRVAFGRTGETLIAIMVAVAALTSINATMIVGARTN"
BASE COUNT          387 a          938 c          969 g          455 t
ORIGIN      
        1 cctgcggcgc ctggccggca tcgccacgcg cgtgatccac ctggcgccgc caccggccga
       61 aggcgagggc gacccgcgca gcgccgcgct gctgcgcgcg ctagcgcgcc gcacgtcgcc
      121 gctggcgctg gtctacgcat cgaccagcgg cgtgtacggc gactgcggcg gcgcctggat
      181 cgacgagaca cgcgcgctgc gcccgcaaac cgcccgtgcg cgccgccgtg tggcggccga
      241 aagcgcgttg cgcgcctttg gccgcgccgc cggcacacgc accagcatcc tgcgtattcc
      301 tggcatctac gcgcccgacc gcgaaggcgg cacgccgcgc gcgcggctgg agcgcggcac
      361 gcccgtgctg gcggcggatg acgacgtgta caccaatcac atccacgccg acgacctggc
      421 gcgcgccgtg gcggcggcgc tgtggcgcgg caagccgcag cgcgcggtca acgccagtga
      481 cgacaccgaa ttgaagatgg gcgactactt cgatctggcg gctgatttgt acggcctgcc
      541 gcgcccaccg cgcatcggcc gcgaccaggc gcgcgagcaa ctggcgccga cgctgctgtc
      601 tttcatgagc gagtcgcgcc gcttgctcaa cgggcgcctc aagcgcgagc tgcgcctgcg
      661 gctggaccac gccacgccgg ccaccggatt gctctctaaa agatagctgc ttgcgcagtc
      721 agaacaagcg ctggaggctt tttttgttgc cgttgttgag tgcagcgccg aggctgacgc
      781 gcgtcggtta ttcttttgcg ctgctgccat cgcttcgtgg aaagaacgca tgacactgat
      841 cgacaaacca cgcgccgatc ggcgcgcctt catcactggt tctgcggcct gggccgccag
      901 cggcttgctg gcctcgggcg ggctgcaggc gcagcaattg cgcgcaggct cgtttgccgc
      961 cggcgactac aagccgcggc gcggccagtc gggcaaggac gtgatctgga tacctacgcc
     1021 cgatctgttc gtcacccgca tgctgcggct ggcgcaggtg acgccgcaag acttcgtaat
     1081 cgacctcggc tcgggcgacg gcaagatcgt gattgccgcc gcgcgcgagg gcgcccgcgg
     1141 gctgggcatt gaatacaacc ccgacatggt cgagctgtcg cgccgccgcg cgcaggaagc
     1201 cggcgtggcc gagcgcacgc gcttcgagaa ggcggacatc ttcgagtccg atttcagcca
     1261 ggcgacggtc gtcaccatgt atttgctgcc gcaactcaac ctgaagctgc gcccgcgcat
     1321 cctggcgctc aagcccggca cgcgtgtggt ctcgcacgaa tttcgcatgg ggcgctggcg
     1381 tccggacgaa acatccaacg tcgatcacgc cgcgctgcac ctgtggctgg tgccggccaa
     1441 cgtgggcggg cagtgggagc tgcagtttcc gcagcaagcc ggcccggtga ccacgcggct
     1501 ggaagtgcgg cgccagcatt ttcaggactt caccggaacg gtcggcttca aggatttcga
     1561 taccggtctg cgcgagccca aggtggccgg cgagcaggtg cgttttggct ttaccgacga
     1621 agacgggcgc ctgcgccgct ttgtgggcca ggtggcgggc gatgtcatca acggcagcgt
     1681 gtacgacctg acggccggcg gcgcgcgcgc ccccttcagc gcccgccgca tcggcgccgc
     1741 gccggccatc gacggctcag gcccggccgc cgcggacgag gccgaatcgc tgggcaacga
     1801 ctgatgcagc cgggctccga aaccacccag ccgcaacaga ccctgcggct gcgcgaggcg
     1861 gtggcgatca tcgtcggcat cgtcatcggc gcgggcatct tcaaggcgcc ttctctggtg
     1921 gcgcagttca ccggctccat cggctggatg ctggccgcgt gggtggccgg cggggtgatc
     1981 tcgctcatcg gcgcgctgtg ctacgccgag ctggccgcgg cctggccgca cgcgggcggc
     2041 gactatcact ttctgcagcg cgcctatggc cggcgggtcg cgtttttgtt tgcgtgggcg
     2101 cgcttttcgg tcatcaccac cggctccatc gccttgctgg ccttcgtgtt tggcgactac
     2161 atgagcgccg ttttgccgct gggcacctac agcgcggcgc tgtggggcgc gttggccgtg
     2221 gtggtactga cctggctcaa cgcgcgcggc attcacgcca gcgccagcgc gcaaagctgg
     2281 ctcacgctgg ccgaggtgct gggcctggtg ctggtcatcg ccgccggcct gtggatgtgg
     2341 agcagcagcg tgcaccgcat gccgatgccc gatggcgctg tggtcgctgg cgccgtttca
     2401 gccatgccgg ggcttggcat gctgggcctg gcgatggtct tcgtgctgct gacctacggc
     2461 ggctggaacg aggccgcgta cctgagcgcc gaactgcgcg gctcgcgccg cggcatgctg
     2521 cacgcgctgg tgatcagcct ggcgttgatc acggtgcttt acctgctggt caacggggct
     2581 tacgtgtacg ggctgggcat tcagggcatg gcaaagagcg aggcggtggc ggccgacctg
     2641 ctgcgcgtgg ccttcgggcg caccggcgag acgctgatcg ccatcatggt ggcggtggcg
     2701 gcgctgacct cgatcaacgc caccatgatc gtcggcgcgc gcaccaact
//
LOCUS       DAOMVO010000320         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10936_length_2743_cov_15.884301, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000320 DAOMVO010000000
VERSION     DAOMVO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10936_length_2743_cov_15.884301"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..246
                     /locus_tag="PKV30_12170"
     CDS             4..246
                     /locus_tag="PKV30_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HRL31003.1"
                     /translation="MSKKNIEECVRESLEAYFRDLRGTEPGALYDMVVRTVEKPLLDV
                     VMQHAGHNQSRAAEWLGMNRNTLRKKLLEHELLSPP"
     gene            346..1950
                     /gene="purH"
                     /locus_tag="PKV30_12175"
     CDS             346..1950
                     /gene="purH"
                     /locus_tag="PKV30_12175"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRL31004.1"
                     /translation="MNALISVSDKTGVAEFAAALVALGFNLLSTGGTAKLLQEAGLPV
                     TEVAEVTQFPEMLDGRVKTLHPKVHGGLLARRDVPAHMAALAEHGIGTIDMLVVNLYP
                     FEATVAKPGCTLADAIENIDIGGPAMVRSAAKNWQDVAVLTDAAQYAPVLAELQAGGG
                     KLSDKTRFALAVAAFNRISNYDAAISDYLSAIDFGAAGASGTPVRGLFAAQSNGRFIK
                     LQDLRYGENPHQQAAFYRDLYPAPGSLVTAEQLQGKELSYNNIADADAAWECVKQFSA
                     PACVIVKHANPCGVAMGADVLEAYQKAFKTDPTSAFGGIIAFNVPVDGAAAEAVSKQF
                     VEVLMAPDFTPEALAVFKAKQNVRVLRISLAGVQPGGASAWDRGLNAMDVKRVGSGLL
                     MQTADNHELTLAELKVVTKKTPTDQQLQDLLFAWKVAKFVKSNAIVFCSGGMTMGVGA
                     GQMSRLDSARIASIKAGHAGLSLQGTAVASDAFFPFRDGLDVVIDQGATCVIQPGGSM
                     RDQEVIDAADERGVAMVFTGVRHFRH"
     gene            complement(2016..2573)
                     /locus_tag="PKV30_12180"
     CDS             complement(2016..2573)
                     /locus_tag="PKV30_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31005.1"
                     /translation="MRKVGIIALALLLAAASGVQARGARVKGSGGSHGSSHSAPHAES
                     SGGGVHLPSVRPRSQGGSEQNQAATGGATNTAAPDSRTAMQDRLNAELAAQRAATPPG
                     APVGTLSAAPKLMEPAPLAANERRVTQRTAVEKTVAVKAMAPADLTALVPKDSAKKAN
                     VDDQRRPDRFNSRGVNCSLYPARCG"
BASE COUNT          473 a          854 c          957 g          459 t
ORIGIN      
        1 accatgagca agaagaacat cgaggagtgc gtgcgcgaaa gcctggaggc gtacttccgc
       61 gacctgcgcg gcaccgagcc gggtgcgttg tacgacatgg tggtgcgcac ggttgaaaag
      121 ccgctgctcg acgtcgtgat gcagcacgcc ggccacaacc agagccgcgc cgccgaatgg
      181 ctgggcatga accgcaacac gctgcgcaag aagctgctcg aacacgagct gctgtcgccg
      241 ccatgatgcg gcagcgccaa agccgaaaac tatgtttttg atagctgctt gcgcttgatg
      301 gacaagcgcc ggcgcctgtt tttatattcg aactcgttgt cagccatgaa cgcattgatc
      361 tccgtttccg acaagaccgg cgtcgccgaa ttcgccgccg cgctggtggc gctgggcttc
      421 aatctgctgt cgaccggcgg cacggccaag ctgctgcagg aggccggcct gccggtgacc
      481 gaggtggccg aggtgacgca gttccccgag atgctcgacg gccgcgtgaa aacgctgcac
      541 cccaaggtgc acggcggcct gctggcgcgg cgcgacgtgc cggcgcacat ggccgcgctg
      601 gccgagcacg gcatcggcac catcgacatg ctggtggtga acctgtaccc gttcgaggcc
      661 acggtggcca agccgggttg cacgctggcc gacgcgatcg agaacatcga catcggcggc
      721 ccggccatgg tgcgcagcgc ggccaagaac tggcaggacg tggcggtgct gaccgacgcc
      781 gcgcagtacg cgccggtgct ggccgagttg caggcgggcg gcggcaagct gtccgacaag
      841 acgcgttttg ccctggccgt ggccgcgttc aaccgcatca gcaattacga cgcggccatc
      901 agcgactatc tgtctgctat tgatttcggt gctgctggcg caagtgggac gcccgtcaga
      961 gggctgtttg ccgcgcaaag caatggccgc ttcatcaagc tgcaagacct gcgctacggc
     1021 gagaacccgc accagcaagc cgccttctac cgcgacctgt accccgcgcc cggctcgctg
     1081 gtgacggccg agcagctgca gggcaaggag ctgagctaca acaacatcgc cgatgccgac
     1141 gcggcgtggg aatgcgtcaa gcagttcagc gcgcccgcat gcgtgatcgt caagcacgcc
     1201 aacccgtgcg gcgtggcgat gggcgcggat gtgctggagg cgtatcaaaa agccttcaag
     1261 accgacccga ccagcgcctt cggtggcatc atcgccttca acgtgccggt cgacggcgcg
     1321 gcggccgaag cggtgtcgaa gcagttcgtc gaggtgctga tggcgcccga tttcacgccc
     1381 gaggcgttgg ccgtgttcaa ggccaagcag aacgtgcgcg tgctgcgcat ttcgctcgcc
     1441 ggcgtgcagc cgggcggcgc cagcgcttgg gatcgcgggc tgaatgccat ggacgtcaag
     1501 cgcgtcggct cgggcctgtt gatgcagacc gccgacaacc acgagctgac gctggccgag
     1561 ctgaaggtgg tgacgaagaa aacgccgacc gatcagcaac tgcaagacct gctgtttgcc
     1621 tggaaggtcg ccaagttcgt caagagcaac gccatcgtgt tctgctcagg cggcatgacc
     1681 atgggtgtcg gcgccgggca gatgagccgg ctcgactcgg cgcgcatcgc cagcatcaag
     1741 gcggggcacg caggcttgtc gctgcaggga acggcggtgg cgagcgatgc gttctttccg
     1801 ttccgcgacg ggctggacgt ggtgatcgac cagggcgcga cctgcgtcat ccagcccggc
     1861 ggttcgatgc gcgatcagga agtgatcgat gcggccgacg agcgcggcgt ggcgatggtg
     1921 ttcactggcg tgcggcactt caggcattga agcgctgccc aaaaacaaga aagcggccaa
     1981 acggccgctt ttcttgttgg ggcgacagca agcctttagc cgcagcgcgc cggatacagc
     2041 gaacagttca cgccccggct gttgaagcgg tcagggcggc gttggtcgtc gacgtttgct
     2101 ttcttggccg agtccttggg caccagtgcg gtcaggtcgg ccggtgccat cgccttcacg
     2161 gcgacggttt tctccaccgc agtgcgttgc gtgacacggc gttcgttggc ggccagcgga
     2221 gccggctcca tcagcttggg cgccgccgac agcgtgccca cgggtgcgcc gggcggcgtg
     2281 gccgcgcgct gggccgccag ttcggcgttg aggcggtcct gcatggcggt gcgcgaatcg
     2341 ggcgcggcgg tgttggtggc gccgccggtc gccgcttggt tctgctcgct gccgccctgg
     2401 ctgcgcgggc gcacgcttgg cagatgaacg ccgccgcccg atgattcggc gtgcggcgcg
     2461 ctgtgcgacg aaccgtgcga gccgcccgag cccttgacgc gcgcgccccg ggcctgcacg
     2521 ccgctggccg cggcgagcaa cagggcgagc gcgatgatgc cgactttcct cataccttcc
     2581 tccgttgatg ggtgcgctgg agaatagcaa cgaggacgga aaacgagaac ctgtagctga
     2641 acgattgagg cgttgaaagc gccgcttgtg tgagaagtgc cacgctcgac tagggcgtgt
     2701 catcgaatta tgaggatgct tcgttatcag gagcatgaat cgc
//
LOCUS       DAOMVO010000321         2741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10950_length_2741_cov_9.489948, whole genome shotgun sequence.
ACCESSION   DAOMVO010000321 DAOMVO010000000
VERSION     DAOMVO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_2741_cov_9.489948"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKV30_12185"
     CDS             complement(<1..1030)
                     /locus_tag="PKV30_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRL31006.1"
                     /translation="MFSLFRRKKTADPAPAMLDPAEAPAPAAPPGVGEAVHTTFVLSD
                     AVDVPLDDLERQALAAGSQNDAADEPPEPADGAAPQGGFMSRWFGGGAAPAPVAPPVS
                     VPAPPPAAPAVAPPIAPPSATNIEAARAELAPIIAEKDLELAPASATAEAARVGWMDR
                     LKTGLKRTGSSISTVFTGTQIDDALYEDLETALLMADAGVPATEFLLQDLKRRVKESR
                     ATDTAQVKGLLRDAVADMLAPLEKTLVVGVYHPTVIMVAGVNGAGKTTSIGKLTKHFV
                     DAGASVLLAAADTFRAAAREQLAVWADRTQVEIITQQGADPSAVAFDAVGAGRARRKD
                     VVLVDTAGR"
     assembly_gap    1217..1316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1317..2378)
                     /gene="selD"
                     /locus_tag="PKV30_12190"
     CDS             complement(<1317..2378)
                     /gene="selD"
                     /locus_tag="PKV30_12190"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRL31007.1"
                     /translation="MTASTSPKTAEPRLTSLSHGGGCGCKIAPGVLSSILRGTTQMPV
                     PPELLVGIETADDAAVYKLNDSQALVATTDFFMPIVDDPFDFGRIAATNALSDVYAMG
                     GTPIMALAVVGMPINVLSVETIGRILEGGASVCRAAGIPIAGGHTIDSVEPIYGLVAL
                     GLVHPDRVARNAAAQPGDVLVLGKPLGVGIYSAALKKEKLGVDGYAHMMAATTRLNTP
                     GPALAALPGVHAVTDVTGFALAGHALELARGAGVTVQLDWSSVPLLPGVRDLAAQGIV
                     TGASGRNWAAYGAEVALPEGFADAERALLTDPQTSGGLLVACAPESLRAVLDVFKAQG
                     FGDAAVVGRVLPKGEKPLVV"
     gene            2545..>2741
                     /locus_tag="PKV30_12195"
     CDS             2545..>2741
                     /locus_tag="PKV30_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRL31008.1"
                     /translation="MHFKPSLSALLLVASASFASAALSQQLAPPAAAASPVSQFKLAN
                     GLTLIVKPDRRAPTAVHMLWVR"
BASE COUNT          402 a          915 c          900 g          424 t
ORIGIN      
        1 ggcggccggc ggtgtcgacc agcaccacgt ccttgcggcg cgcgcggccg gcgccgacgg
       61 cgtcgaaggc gacggccgac gggtcggcgc cttgctgcgt gatgatctcg acttgcgtgc
      121 gatcggccca cacggcgagc tgctcgcgtg cggcggcgcg aaaagtgtcg gccgccgcca
      181 gcagcacgct ggcgccggca tcgacgaagt gcttggtcag cttgccgatg ctggtcgtct
      241 tgccggcgcc gttgacgccc gccaccatga tcaccgtagg gtggtagacg cccaccacca
      301 gcgtcttttc aagcggcgcg agcatgtcgg ccacggcgtc gcgcaacagg cccttgacct
      361 gcgcggtgtc ggtggcgcgc gactccttca cgcggcgctt taagtcctgc agcagaaact
      421 cggtcgccgg cacgccggca tcggccatca gcagcgcggt ttccaggtct tcatacagcg
      481 cgtcgtcgat ctgcgtgccg gtgaagacgg tgctgatgct gctgccggtg cgtttcagcc
      541 cggtcttgag acggtccatc cagcccacgc gcgctgcttc ggcggtggcg gaggcggggg
      601 cgagttcaag gtctttttcg gcgatgatcg gcgccagttc agcgcgagca gcttcgatgt
      661 ttgtagcgct tgggggcgcg atgggaggtg cgaccgccgg cgccgcaggc ggtggcgctg
      721 gcacgctgac cggcggcgca acgggcgcag gcgccgcgcc gccgccaaac cagcgcgaca
      781 tgaagccgcc ctgcggtgcg gcgccgtctg ccggctcggg cggctcatcg gccgcgtcgt
      841 tctgcgaacc cgcggccagc gcctggcgct ccagatcgtc cagtggcaca tccacggcat
      901 cgctcagcac gaaggtggtg tgcaccgcct cgcccacgcc cggcggcgct gcgggcgccg
      961 gcgcctcggc gggatccagc atggcgggcg ctggatcggc ggttttcttg cggcggaaaa
     1021 gactgaacat gaaacgggca gggtctggag tcgccccatt ctaggaggcg gccatgcgag
     1081 cggcagcgac cgcgaccggc tttactatga ataacggagc tactggcgct tgccagacat
     1141 gcgctgcaag ccgatttggc tttgaatcag gtggcgcccg tcttggcgaa gtagcgcacg
     1201 ccgggcaggt cggcgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacc
     1321 accagaggct tctctccctt gggcagcacg cgccccacca ccgccgcatc gccaaacccc
     1381 tgcgccttga acacatccag cacggcccgt aggctttccg gcgcgcaggc caccaacaac
     1441 ccgccgctgg tctgcggatc ggtcagcagg gcgcgctcgg catcggcaaa gccttccggt
     1501 aacgcgactt cggcgccgta agccgcccag ttgcgccccg aagcaccggt gacgatgccc
     1561 tgcgccgcca gatcgcgcac gccgggcagc agcggcacgc tgctccaatc gagttgcacc
     1621 gtcacgccgg cgccgcgcgc cagctccagc gcgtggccgg ccagcgcgaa gccggtgacg
     1681 tcggtgacgg cgtgcacgcc cggcagggcg gccagcgccg ggccgggggt gttcaggcgc
     1741 gtggtggcgg ccatcatgtg ggcgtaaccg tcgacgccca gcttttcctt cttgagcgcc
     1801 gccgaataga tgcccacgcc gagcggtttg cccaacacca acacatcgcc tggctgcgcg
     1861 gcggcgttgc gcgccacgcg gtccgggtgc accaggccga gcgcgaccag gccatagatc
     1921 ggctcgaccg aatcgatggt gtggccgccc gcaatcggga tgcccgccgc gcggcacacg
     1981 ctggcgccgc cttccagaat cctgccaatc gtctcgaccg acagcacgtt gatcggcatg
     2041 cccaccacgg ccagcgccat gatcggcgtg ccgcccatgg cgtacacgtc gctgagcgcg
     2101 ttggtggcgg cgatgcggcc aaagtcgaac gggtcgtcga cgatgggcat gaagaaatcg
     2161 gtggtcgcca ccagcgcctg gctgtcgttg agcttgtaaa cggcggcgtc gtcagccgtt
     2221 tcgatgccga ccagcaactc cggcggcacc ggcatttgcg tggtgccgcg caggatgctg
     2281 gacaacacgc ccggtgcgat cttgcagccg caaccaccgc cgtggctgag cgaggtgagg
     2341 cggggttcgg cggtcttggg ggaggtcgaa gcggtcatgc gatcgattgt cgcgcatgcc
     2401 catgaactat tgaatcgata gctgcccgcg cttgataggc aagcgctggc gccgtaaaag
     2461 cgtcagaatc ggcgccctgg cgcgctcagg cacactcgca ccttcaagga acgaatttcc
     2521 acccctccga ctctgtctgc ggtcatgcat ttcaaacctt ctctttctgc gctgctgctg
     2581 gtcgccagtg cctcttttgc gtccgccgcg ctgtcacaac aactggctcc gccggcagcc
     2641 gctgcgagcc cggtcagcca attcaagctg gccaacggcc tcaccctcat cgtcaagccc
     2701 gaccgccgcg cgcccaccgc ggtgcacatg ctgtgggtgc g
//
LOCUS       DAOMVO010000322         2736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_10980_length_2736_cov_17.776576, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000322 DAOMVO010000000
VERSION     DAOMVO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10980_length_2736_cov_17.776576"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKV30_12200"
     CDS             complement(<1..704)
                     /locus_tag="PKV30_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRL31009.1"
                     /translation="MELALICLASLLAGGIDSIVGGGGLILVPALFAVYPSAAPATLF
                     GTNKSASVWGTSIATVQYARRVQMRWAVMLPAAGAALVGAFIGAWVVTLIDPSFLRRL
                     LPFILLAVLLYTLAKKDLGRTHAPRHTAGRETLLACAVGLIIGWYDGFFGPGTGSFFI
                     FLFVRLLGYDFLNASASAKLLNVATNLSALALFAMKGHVWWKIGLVMAVANVAGSLIG
                     TRLALKHGAGFVRGVF"
     gene            complement(1104..2456)
                     /gene="argG"
                     /locus_tag="PKV30_12205"
     CDS             complement(1104..2456)
                     /gene="argG"
                     /locus_tag="PKV30_12205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRL31010.1"
                     /translation="MSHTILQSLPAGQKVGIAFSGGLDTSAALLWMRQKGAIPYAYTA
                     NLGQPDESDYDAIPRKAMEYGAEKARLVDCRMQLASEGIAALQCGAFHIRTGGATYFN
                     TTPLGRAVTGTMLVNAMKEDDVNIWGDGSTYKGNDIERFYRYGLLANPSLKIYKPWLD
                     QRFIDELGGRKEMSEFMQKAGFDYKMSTEKAYSTDSNMLGATHEAKDLEELSSGIRIV
                     NPIMGVAFWKPEVDVKPEEVRVRFHEGRPVALNGQDIADPVALFLEANAIGGRHGLGM
                     CDQIENRIIEAKSRGIYEAPGMALLHIAYERLLSGIHNEDTIEQYRINGLKLGRLLYQ
                     GRWFDPQAIMLRETAQRWVAAAVTGEVTLELRRGNDYSILNTESPNLTYAPERLSMEK
                     VENAPFTPLDRIGQLTMRNLDITDTRGKLAVYSQSGLLQLGEGSMLPQLGHDSGAGKK
                     "
BASE COUNT          478 a          866 c          894 g          498 t
ORIGIN      
        1 atgaacacgc cgcgcacaaa gccggcgccg tgcttgagcg ccagccgcgt gccgatcagg
       61 ctgcctgcca cgttggccac ggccatcacc aggccgatct tccaccacac atggcccttc
      121 atggcgaaca gcgccagcgc cgacaggttg gtcgccacgt tcagcagctt ggccgaggcc
      181 gaggcgttaa gaaagtcgta gcccaagagg cgcacgaaca ggaagatgaa gaagctgccc
      241 gtgcctggcc cgaagaagcc gtcgtaccag ccgatgatga gaccgacggc gcaggccagc
      301 agcgtctcgc gcccggccgt gtggcgcggc gcgtgggtgc ggcccaggtc ctttttcgcc
      361 agcgtgtaga gcagcaccgc cagcaggatg aagggcagca ggcggcgcag aaagctcggg
      421 tcaatcagcg tgaccaccca ggcgccgatg aacgcgccca ccagcgctgc gccggccgcc
      481 ggcagcatca ccgcccagcg catctgcacg cggcgcgcgt attgcacggt ggcgatgctg
      541 gtgccccaga ccgaggcgct tttattggtg ccgaacagcg tggccggcgc ggcgctgggg
      601 tagaccgcaa acagcgccgg caccaggatc agcccgccgc cgcccacgat ggaatcgatg
      661 ccgccggcca gcagcgaagc caggcagatg agcgccagtt ccatcgaaag atcgctttgt
      721 cgctgtcaaa ttgatagctg ccagcgcaag caggaaaggc gccagtggcc gaaaagacct
      781 tgattatgcg ggctgaagcg tgcgccgcgt gcaagcgcat gggacgaaaa aaaaagagcg
      841 cctttgcggg gcgctcctga gagtcatctg cgaattgcta tgaagaaatc ttgctttgaa
      901 ggcttcgggc ctttcgggtg gtggtctggc ttgtctcctc gggcccttcg agccgcggcc
      961 gcagccgacg acgctagagt catcacaatg taacagcggc caggcacttc ctgtattggg
     1021 gctaccccgt acgaaattgg tgcgcaatcc gcccgattgg cgtgcgaacc attcaaattg
     1081 agtgcttttg actgaacgtc ggcttacttc ttgcccgcgc cgctgtcgtg ccccaactgc
     1141 ggcagcatcg agccttcgcc cagttgcagc agcccgctct ggctgtacac ggccagcttg
     1201 ccgcgcgtgt cggtgatgtc caggttgcgc atggtcagct ggccgatgcg gtcgagcggc
     1261 gtgaaggggg cgttttccac cttttccatc gacaggcgct cgggcgcgta ggtcaggttg
     1321 ggcgattcgg tgttgagaat gctgtagtcg ttgccgcggc gcagctccag cgtcacctcg
     1381 cccgtcacgg ccgccgccac ccagcgctgc gccgtctcgc gcagcatgat ggcctgcggg
     1441 tcgaaccagc ggccctggta cagcaggcgg cccagcttga ggccgttgat gcggtactgc
     1501 tcgatggtgt cctcgttgtg gatgccggac agcagccgct cgtaggcgat gtgcagcagc
     1561 gccatgccgg gcgcttcgta aatgccgcgc gacttggctt cgatgatgcg gttttcgatc
     1621 tggtcgcaca tgcccagccc gtggcgaccg ccaatggcgt tggcttccag aaacagcgcc
     1681 accgggtcgg caatgtcctg cccgttcagc gccaccgggc ggccttcgtg gaagcgcacc
     1741 cgcacctctt ccggcttcac gtcgacctcg ggcttccaga acgccacacc catgatcggg
     1801 ttgacgatgc ggatgccgct gctcaactct tccaagtcct tcgcctcgtg cgtggcgccc
     1861 agcatgttcg aatcggtcga ataggccttt tcggtcgaca tcttgtagtc aaaaccggcc
     1921 ttctgcatga attccgacat ctccttgcgg ccgcccagct cgtcgatgaa gcgctggtcg
     1981 agccagggct tgtagatctt cagcgacggg ttcgccagta agccgtagcg gtagaagcgc
     2041 tcgatgtcat tgcccttgta tgtgctgcca tcgccccaga tgttgacgtc atcttccttc
     2101 atcgcgttga ccagcatggt gccggtcacc gcgcggccca gcggcgtggt gttgaagtag
     2161 gtggcgccgc cggtacggat gtgaaaggcg ccgcactgca gcgcggcaat gccttcgctg
     2221 gccaactgca tgcggcaatc gaccaggcgc gccttttcgg cgccgtattc catcgccttg
     2281 cgcggaatcg cgtcgtagtc cgattcgtcc ggctggccca ggttggcggt gtaggcgtag
     2341 gggatggcgc ccttctggcg catccacagc agcgcggcgg aggtatccag gccgcctgaa
     2401 aaggcgatgc cgaccttctg gccggcggga agcgattgga ggatggtgtg ggacatgggc
     2461 tggcggcggg cggtaccgcg tcgatcaaag gtgagggtga acagggaagc cggctattgt
     2521 ccgacctttc cggccgcagc cgccgggcgc cgtgcggcca aggctgcttt taagcgaagt
     2581 tgaccgctta tccttactaa tcaagcgccg gcagctattt atacgatagc agtcgtcggc
     2641 aaatcgacag gccgcgccgc aacgccttca cactgcgccg cacaatctgc cggcttgcgg
     2701 cggcggccag cagcggggcg aagaggtcac gctggt
//
LOCUS       DAOMVO010000323         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11131_length_2711_cov_16.600527, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000323 DAOMVO010000000
VERSION     DAOMVO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11131_length_2711_cov_16.600527"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1281)
                     /locus_tag="PKV30_12210"
     CDS             complement(1..1281)
                     /locus_tag="PKV30_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL31011.1"
                     /translation="MDRRQFIQAGSAASVLGVAAGCASTGGGSGAGGNVVVVGGGYGG
                     ATAAKYIRMWSEGRVNVTLVEPNDSFISCPISNLVIGGSKQMADITTPYDNLAKRHGV
                     KIIKDRAVSIDAGKRTITLAGGTVLPYDRLVLSPGIEFMWDALPGLKKPGAQDKVLHS
                     WKAGPQTVALRRQLEAMPDGGVYALTIPLSPYRCPPGPYERACQVAHYFSKAKPKSKV
                     LILDANDDVTSKGPLFKKAWADRYKGIIDYRPKHNLTDVDAATGTLKFEFNDDLKAQV
                     LNVVPPQRAGDIAHNAGLTTANKRWCEVDWQTMESKAAKNIHILGDALQVAPLMPKSG
                     HMANQHGKVAAAAIVALLSGRAPNALPIYNNTCYSFVSDTDVVHVASVHRYDDAQKTM
                     LTVQGSGGVSAAASELEGKYAMAWARNIWADSLA"
     gene            complement(1293..1604)
                     /locus_tag="PKV30_12215"
     CDS             complement(1293..1604)
                     /locus_tag="PKV30_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I cytochrome c"
                     /protein_id="HRL31012.1"
                     /translation="MKPSLFTGLAAAALCLAAGAAHAQVDPLQVRSWAAACANCHGTN
                     GKAQPGNEPLAGQNKDEMMKKLMDFKSGAKPATIMHQLAKGYTDEQLAAITAWFAAQK
                     K"
     gene            complement(1744..2088)
                     /locus_tag="PKV30_12220"
     CDS             complement(1744..2088)
                     /locus_tag="PKV30_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRL31013.1"
                     /translation="MVKKDTPDAPDSELESDEVFELAAEVFRVMSAPMRLKIISALCN
                     GEKNVGELLADIHTTQPNMSQHLNTLYKAGVLGRRRDGVQIYYRIINERVVTLCRAVC
                     TQIAIETVDEAR"
     gene            complement(2118..>2711)
                     /locus_tag="PKV30_12225"
     CDS             complement(2118..>2711)
                     /locus_tag="PKV30_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRL31014.1"
                     /translation="LAYSLRRAPTLLRRAAVQVVSLGYAVPGAVIVVGLLLPVGWLQA
                     RWPDSGIGAVVTVTVWGLVWAYLVRFVSVALQSMQSGYARVPRSLDESARMLGAGGVE
                     LAARVHWPLLRRAGFAAALLVFVDVMKELPATIVLRPFNTDTLAVVANQLARDERLGE
                     AALPSLALVLVGLVPVILLSRTLRVDRRPLPPAEAQV"
BASE COUNT          423 a          904 c          908 g          476 t
ORIGIN      
        1 tcaggccagg ctatcagccc agatgttgcg cgcccacgcc atggcgtact tgccttccag
       61 ctcgctcgcc gccgccgaca cgccgcccga gccctgcacg gtaagcatgg tcttttgcgc
      121 gtcgtcataa cgatgcacgc tggccacgtg gacgacgtcg gtgtcgctga cgaagctgta
      181 gcaggtgttg ttgtagatcg gcagcgcgtt gggcgcgcgg cccgacagca gcgcgacgat
      241 ggcggccgcc gccaccttgc cgtgctggtt ggccatgtgg cccgatttgg gcatcaatgg
      301 cgccacctgc agcgcgtcgc ccaggatgtg gatgttcttg gcggccttgg attccatggt
      361 ctgccaatcg acctcgcacc agcgcttgtt ggcggtggtc aggcccgcgt tgtgggcaat
      421 gtcgcccgcg cgctggggcg gcacgacgtt cagcacctgc gccttcaggt cgtcgttgaa
      481 ctcgaacttg agcgtgccgg tggcggcatc gacgtcggtc aggttgtgct tgggccggta
      541 gtcgatgatg cctttgtagc ggtcggccca ggccttcttg aacagcggcc ccttggacgt
      601 gacgtcgtcg ttggcgtcca ggatcagcac cttggacttg ggtttggcct tgctgaagta
      661 gtgcgccacc tggcaggcgc gctcgtaggg ccctggcggg cagcggtagg gcgacagcgg
      721 aatggtcagc gcatagacgc cgccatcggg catggcttcg agctgccggc gcaaagccac
      781 ggtttgcggg ccggccttcc acgaatgcag caccttgtct tgcgcgccgg gctttttcag
      841 gccgggcagc gcgtcccaca tgaattcgat gccaggcgac agcaccaggc ggtcgtatgg
      901 cagcaccgtg ccaccagcca gcgtgatggt gcgcttgccg gcgtcgatgg agaccgcgcg
      961 gtccttaatg atcttcacgc cgtgacgctt ggccaggttg tcgtagggcg tggtgatgtc
     1021 ggccatctgc ttgctgccgc cgatgaccag gttggagatc gggcaagaga tgaagctgtc
     1081 attgggctcg accagcgtga cattgacgcg cccctcagac cacatgcgga tgtacttggc
     1141 cgccgtggcg ccgccgtagc cgccgccgac gacgaccacg ttgccccccg cgccgctgcc
     1201 accgcccgtg gaggcgcaac ccgcggccac gccgagcacc gatgcggcag agccagcctg
     1261 aatgaattga cgacgatcca tggtgtgcct cctcacttct tctgcgccgc gaaccacgcg
     1321 gtgatggcgg cgagctgctc gtcggtgtag cccttggcca gctgatgcat gatggtcgcg
     1381 ggcttggcgc cgctcttgaa gtccatcagc ttcttcatca tctcgtcctt gttctggccg
     1441 gccagcggct cgttgccagg ctgcgctttg ccgttggtgc cgtggcagtt ggcacaggca
     1501 gcggcccagc tgcgcacctg cagcggatcg acttgggcat gggccgcgcc ggcggccagg
     1561 cacagggccg cggcggcaag ccctgtaaac aaggaaggtt tcatgacgtg ggatcctcca
     1621 gaggggtggc cgatggacgc gaacggcgat caattcgccg ttgcttatat gtaatcggat
     1681 gatacggatc gaggccggca gcgtcgtcgg ggattacccc agcgcatcgg tgcgcggccc
     1741 gaatcaacgc gcctcgtcca ctgtttcaat ggcgatttgc gtgcacaccg cgcggcacag
     1801 cgtgaccacg cgttcgttga tgatgcggta atagatctgc acgccatcgc gccgccggcc
     1861 cagcacgccc gccttgtaca aggtgttgag gtgctggctc atgttgggct gcgtggtgtg
     1921 gatgtcggcc aacagctcgc cgacgttctt ttcgccattg cacagcgcgc tgatgatctt
     1981 cagccgcatc ggcgccgaca tgacgcggaa cacttcggcg gccagctcga agacctcgtc
     2041 cgattcaagt tcgctgtcgg gggcgtcggg ggtgtctttt ttcaccatga tcgggcttca
     2101 ggcagtggcg ctgcccacta tacctgcgcc tcggcaggcg gcaagggccg ccgatcgacg
     2161 cgcaacgtgc ggctgagcag aatcaccggc accagcccca ccagcaccag cgccagcgag
     2221 ggcaacgctg cttcgcccag ccgctcgtcg cgcgccagct ggttggccac cacggccagc
     2281 gtgtcggtgt tgaaggggcg cagcacgatg gtggccggca gttccttcat cacgtcgacg
     2341 aacaccagca gcgccgccgc aaagccggcg cgccgcagca gcggccagtg cacgcgcgcc
     2401 gccagttcca cgccgccggc gcccagcatg cgggccgatt cgtccaggct gcgcggcacg
     2461 cgggcgtagc cgctctgcat cgactgcagt gccaccgaga caaagcgcac cagataggcc
     2521 cagaccagcc cccacaccgt gacggtgacc accgcgccga tgccgctgtc cggccagcgc
     2581 gcctgcagcc agccgaccgg cagcagcagg ccgaccacga tcaccgcgcc cggcaccgcg
     2641 tagcccagcg acaccacctg cacggccgcg cggcgcagca gcgtgggcgc gcggcgcagg
     2701 ctgtaggcca g
//
LOCUS       DAOMVO010000324         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11185_length_2701_cov_12.215042, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000324 DAOMVO010000000
VERSION     DAOMVO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11185_length_2701_cov_12.215042"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PKV30_12230"
     CDS             complement(<1..1449)
                     /locus_tag="PKV30_12230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRL31015.1"
                     /translation="MNDQTVLDLTGDGSAPDDDLALATYAQRAYLEYALSVVKGRALP
                     DVADGQKPVQRRILYAMDRMGLAYGGAGGNTAATPVKSARVVGDVLGKYHPHGDSAAY
                     DALVRMAQDFSQRYPLIDGQGNFGSRDGDGAAAMRYTEARLSKITRLLLEEIDEGTVD
                     FQPNYDGREQEPRQLPARLPFALLNGASGIAVGLATEIPSHNLREVADACVALIKTPK
                     LTDEELFVLIPGPDYPGGGQIISAASDIQDAYKSGRGSLKVRARWKIEDLARGQWQLV
                     VTELPPGASTQKVLEEIEELTNPKVKTGKKAVTQEQNQLKATVLSVLDEVRDESSKDA
                     PVRIVFEPKSRTVGQDELIHTLLAHTSLETSASINLTSVGLDGRPTQKSLRQMLVEWI
                     EFRQQTITRRSEHRLQKVLDRIHILEGRQLVLLNIDEVIAIIRNSDEPKAALIARFKL
                     SDRQADDILDIRLRQLARLEAIKIEQELSELRK"
     gene            complement(1472..2425)
                     /locus_tag="PKV30_12235"
     CDS             complement(1472..2425)
                     /locus_tag="PKV30_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRL31016.1"
                     /translation="MHLSAHADLWTYVDEKGITHFAATQVDERYALFYKGGDIAKLDL
                     VGSAAASPARAQSRVGLKASGKTGEPKFEVPKRFAKLDSSTGYKSVQKHLQAAAKTHK
                     VDYELLKAVIAAESGFDPQAVSPKGAVGLMQLMPPTAEQYGVLADKEGRKDRKGKLLP
                     ARSVEEKLTDPQTNINAGARYLAYLIKLFKGEMELAVAAYNAGEGAVQRAGNKIPKFK
                     ETQGYVKTVMGLYGVFKPEDKTTLLATSRAVPAAIGTRAGGRVRVELNGGGRAFEAAK
                     PAPSTSAADAAAAGVNALTPVLFSVPTLGADVRVSNSAAGS"
     gene            complement(2509..>2701)
                     /locus_tag="PKV30_12240"
     CDS             complement(2509..>2701)
                     /locus_tag="PKV30_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31017.1"
                     /translation="EMNAEQLWETTLNPDTRRLLPVRLGRFDFGQTTAEMTKLMGKGE
                     AASRRELMELHGDAVDIDI"
BASE COUNT          405 a          878 c          889 g          529 t
ORIGIN      
        1 cttgcgcagc tcggacagtt cctgctcgat cttgatggct tcgagccgcg ccagttggcg
       61 caggcggatg tcgaggatgt cgtcggcctg ccgatctgac agcttgaagc gcgcgatcag
      121 cgccgccttc ggctcgtcgc tgtttcggat gatcgcaatc acctcgtcga tgttcagcag
      181 caccagctgc cggccttcga ggatgtggat gcggtccagc accttttgca gccggtgctc
      241 gctgcggcgc gtgatggtct gctggcggaa ctcgatccac tccaccagca tctgccgcag
      301 cgacttttgc gtggggcggc cgtccaggcc gacgctggtc aggttgatcg aggcggatgt
      361 ttccaggctg gtgtgcgcca gcaacgtgtg gatcaactca tcttgcccga cggtgcggct
      421 tttgggctcg aacacgatgc gcaccggcgc gtccttgctc gattcgtcgc gcacctcgtc
      481 cagcaccgac agcacggtgg ccttgagctg gttctgctcc tgcgtcaccg ccttcttgcc
      541 ggtcttgacc ttggggttgg tcagttcctc gatttcttcc agcacctttt gcgtcgaggc
      601 gccgggcggc agttcggtca cgaccagttg ccactggccg cgcgccaggt cttcgatctt
      661 ccagcgcgca cgcactttca ggctgccgcg gccgcttttg tacgcatcct gaatgtcact
      721 ggcggcgctg atgatttgcc cgccgcccgg atagtcggga ccggggatga gcacgaacag
      781 ctcttcatcg gtcagtttcg gggtcttgat gagcgccacg caggcatcgg ccacttcgcg
      841 caggttgtgg ctcgggattt cggtcgccag gccgacggcg atgccgctgg cgccattgag
      901 cagcgcaaac ggcaggcgcg cgggcaactg gcgcggctcc tgctcgcggc cgtcgtaatt
      961 gggctggaaa tcaaccgtgc cttcgtcaat ctcttcgagc agcaggcgcg tgattttgga
     1021 caggcgcgcc tcggtgtagc gcatggccgc ggcgccgtcg ccgtcgcggc tgccgaagtt
     1081 gccctggccg tcgatgagcg ggtaccgctg gctgaaatcc tgcgccatgc gcaccaacgc
     1141 gtcgtaagcg gcgctgtcgc cgtgcgggtg gtacttgccc agcacgtcgc ccaccacgcg
     1201 ggcgcttttg accggcgtgg cggccgtgtt gccgccggcg ccgccgtagg ccagacccat
     1261 gcgatccatc gcgtacagga tgcggcgctg caccggcttc tggccatcgg ccacgtcggg
     1321 cagcgcgcgg cccttgacga ccgacagcgc gtattcaagg taagcgcgct gcgcgtaagt
     1381 ggccagcgcc aggtcgtcgt ccggggcgct gccgtccccg gtcaagtcca ggaccgtttg
     1441 atcgttcatg cgggaaaacg gaagaaggga ttcagctgcc ggcggcagag ttgctcacgc
     1501 gcacatcggc gcccagcgtg ggcacgctga acaagaccgg ggtcagcgcg ttcacgccgg
     1561 cggcggcagc gtcggccgcc gaagtcgaag gcgccggctt ggccgcctcg aacgccctgc
     1621 cgccgccatt cagctcgacg cgcacgcgcc cacccgcccg cgtgccgatg gctgccggca
     1681 cggcgcggct ggtggccagc agcgtggtct tgtcctcggg cttgaagaca ccgtaaaggc
     1741 ccatcacggt cttcacgtag ccctgggttt ccttgaactt ggggatcttg ttgccggcgc
     1801 gctgcaccgc gccttcaccg gcgttgtagg cggccacggc caattccatc tcacccttga
     1861 acagcttgat caagtaggcc aggtagcgcg cgccggcgtt gatgttggtt tgcgggtcgg
     1921 tcagtttctc ttcgaccgag cgcgcaggca gcaacttgcc cttgcggtcc ttgcggcctt
     1981 ccttgtcggc cagcacgccg tactgctcgg ccgttggcgg catcaattgc atcaagccga
     2041 cggcgccctt gggcgacacg gcctgcgggt caaagcccga ttcggcggcg atcacggctt
     2101 tcagcaactc gtaatcgacc ttgtgcgtct tggcggccgc ctgcaggtgc ttttgcaccg
     2161 acttgtagcc ggtcgatgaa tcgagcttgg caaagcgctt gggcacctcg aacttcggct
     2221 cgccggtctt gccgctggcc ttcaggccca cgcgcgactg cgcgcgggca ggtgaagcgg
     2281 cggcgctgcc cacgagatcc agcttggcga tgtcgccgcc cttgtagaac agcgcgtaac
     2341 gctcgtcgac ctgggtggcg gcgaaatggg tgatgccctt ctcatcgacg taggtccaca
     2401 gatcggcgtg cgccgagaga tgcaaaaatg agagcgcaag acccaatact ggcaagcgcc
     2461 agcggccaaa aaaacccttg aaaacggaag caggtggcat gcgtcggttc agatgtcgat
     2521 gtccacggcg tcgccgtgca gttccatgag ctcgcgccgc gaagcggctt cgcccttgcc
     2581 catgagcttg gtcatttccg ccgtggtctg gccaaagtca aaacggccca gtcgcaccgg
     2641 cagcaggcgg cgggtgtcgg gattgagcgt ggtttcccac aattgttccg cgttcatctc
     2701 g
//
LOCUS       DAOMVO010000325         2697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11209_length_2697_cov_15.883043, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000325 DAOMVO010000000
VERSION     DAOMVO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11209_length_2697_cov_15.883043"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /gene="recG"
                     /locus_tag="PKV30_12245"
     CDS             <1..1916
                     /gene="recG"
                     /locus_tag="PKV30_12245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRL31018.1"
                     /translation="QIEGEVTHQEVTFRPRRQLLVSVNDGSDTVVLRFFNFYPSQQKQ
                     MAVGSRIRARGELRGGFAGLTMMHPTVRAAGGALPAALTPVYPTVAQLSQPYLRKAVQ
                     AGLARADLSESLPEAVLNEIGPLRLWDLRKALSFLHQPTPDVSLAELEDHSHPAWQRL
                     KAEELLAQQLSQLEARRAREHLRAPVLRGAPGGLAEQLLAALPFQLTGAQRRVGEEIA
                     HDLARAQPMHRLLQGDVGSGKTVVAALAACIAIDAGWQCALMAPTEILAEQHFAKLVE
                     WLAPLLAARGQRVAWLTGSQKKKARGEMLALIDSGEAALVVGTHAVIQEQVRFARLGL
                     AIIDEQHRFGVQQRLDLRRKLSEAQQEPHLLMMSATPIPRTLAMSYYADLDVSTLDEL
                     PPGRTPVVTKLVAAHRRAEVIERIRAQIAEGRQVYWVCPLIEESEALDLRNATETHAE
                     LSQALPGQMIGLLHSRMPTAEKKAVMALFTAGDMPVLVSTTVIEVGVDVPNASLMVIE
                     HAERFGLSQLHQLRGRVGRGAAASACVLLYEAPEGGRLGETARARLKAMVETNDGFEI
                     ARRDLDIRGPGEFLGARQSGAALLRFADLAEDSALLQWARAAAPRMLAEHPDLAAQHV
                     ARWLGSKSDYLKA"
     gene            1960..2238
                     /locus_tag="PKV30_12250"
     CDS             1960..2238
                     /locus_tag="PKV30_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31019.1"
                     /translation="MLDQFVKMTQRIIAEDGFDNYLPTLLLPATREVRVLDQVEGDGE
                     SLGITDWLAEQVGPDEDFLVAFKAGDAHFMVVGRLGGQACERLCAVGA"
     gene            2252..>2697
                     /locus_tag="PKV30_12255"
     CDS             2252..>2697
                     /locus_tag="PKV30_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL31020.1"
                     /translation="MTLTELKYIVAVAREKHFGKAAEACFVSQPTLSVAVKKLEDELE
                     LKLFERSAGEVTVTPLGDEIVRQAQSVLEQAAEIKEIAKRGKDPLGGPLKLGVIYTIG
                     PYLLPDLVRQNIALTPQMPLMLQENFTVRLLEMLRTGEIDCAILAEP"
BASE COUNT          438 a          870 c          967 g          422 t
ORIGIN      
        1 tgcagatcga gggcgaggtg acgcaccagg aggtgacgtt tcgcccgcgc cgccagttgc
       61 tggtgagcgt gaacgatggc agcgacaccg tggtgctgcg ctttttcaac ttctacccct
      121 cgcagcaaaa gcagatggcg gtgggcagcc gcatccgcgc gcgcggcgaa ctgcgcggcg
      181 gttttgccgg cctgaccatg atgcacccca cggtgcgcgc ggccggcggc gcgctgcccg
      241 cggcgctgac gccggtgtac ccgacggtgg cgcaactgtc gcagccctac ctgcgcaagg
      301 cggtgcaggc cgggctggcg cgggccgatc tttcggaatc gttgccggag gcggttttga
      361 atgaaatcgg ccccttgcgc ttgtgggact tgcgcaaggc gctctcattt ttgcatcaac
      421 cgacgcccga tgtgtcgctg gccgagctgg aagaccacag ccacccggcc tggcagcggc
      481 tgaaggccga ggaactgctg gcgcagcagt tgtcgcagct ggaggcacgc cgcgcacgcg
      541 agcatctgcg tgcgccggtg ctgcgcggcg cgccgggcgg cttggccgag cagttgctgg
      601 ctgcgctgcc gtttcagctg accggcgcgc agcgccgcgt gggtgaggag atcgcgcacg
      661 acctggcccg cgcgcaaccc atgcatcgtt tgctgcaagg cgacgtcggc agcggcaaga
      721 cggtggtcgc cgcgctggcg gcttgcatcg ccatcgacgc cggctggcaa tgcgcgctga
      781 tggcgccgac cgagatactg gccgagcagc actttgccaa gctggtcgaa tggctggcgc
      841 cgctgctggc cgcgcgcggc cagcgcgtgg cgtggctgac cggcagccag aagaaaaagg
      901 cgcgcggcga gatgctggcg ctgatcgact ccggcgaggc ggcgctggtg gtgggcacgc
      961 acgccgtcat ccaggagcag gtgcgctttg cccggctggg gctggccatc atcgacgagc
     1021 agcaccgttt tggcgtgcag caaaggctgg acttgcggcg caagctatca gaagcgcagc
     1081 aagaacccca cctgctgatg atgagcgcca cccccatccc gcgcacgctg gcgatgagct
     1141 actacgccga tctggatgtc tccacgctgg acgaactgcc gcccggccgc acgccggtcg
     1201 tcaccaagct ggtggcggcg caccggcgtg ccgaggtgat cgagcgcatc cgcgcgcaga
     1261 tcgccgaagg ccggcaggtg tactgggtct gcccgctgat cgaagaaagc gaggcgctgg
     1321 atttgcgcaa tgccaccgaa acgcacgccg aactgagcca agccttgccc ggccagatga
     1381 tcggcctgtt gcactcgcgc atgccgacgg ccgagaagaa ggcggtgatg gcgctgttca
     1441 ccgccggcga catgccggtg ctggtcagca ccaccgtcat tgaggtcggc gtggatgtgc
     1501 ccaacgccag cctgatggtg atcgaacacg ccgagcgctt tgggctgtcg caactgcatc
     1561 aactgcgcgg ccgcgtgggt cggggcgccg ccgcctctgc ttgcgtgctg ctgtacgagg
     1621 cgcccgaagg cggccgcctg ggcgagacgg cgcgcgcgcg cctcaaggcg atggtggaga
     1681 cgaacgacgg cttcgagatc gcccggcgcg atctggacat tcgcggcccg ggcgaattcc
     1741 tgggcgcccg ccagtccggc gccgcgctgc tgcgctttgc cgatctggcc gaagacagcg
     1801 cgctgttgca atgggcgcgt gccgccgcgc cgcgcatgct ggccgaacat cccgatctgg
     1861 cggcgcagca cgtggcgcgc tggttggggt caaaatccga ctatctgaaa gcgtgagccg
     1921 cttcggctca cccacaatgc gctgcgacgg aactcgccca tgcttgatca attcgtgaag
     1981 atgactcagc ggatcattgc cgaagacggc ttcgacaact atctgcccac cctgctgctg
     2041 ccggccacgc gcgaggtgcg ggtgctggac caggtggagg gcgacggcga atcgctcggc
     2101 atcaccgact ggctggccga gcaggtcggg ccggacgagg acttcctggt cgccttcaag
     2161 gccggcgacg cgcacttcat ggttgtcggc cggctcggcg ggcaggcgtg cgagcgtttg
     2221 tgtgcggttg gggcgtgagc ggaactgcag catgactctc accgagctca aatacatcgt
     2281 cgccgtggcc cgcgaaaagc actttggcaa agccgccgaa gcctgcttcg tctcgcagcc
     2341 gacgctgtcg gtggcggtga agaagctcga ggacgagctg gagctgaagc tgttcgagcg
     2401 cagcgcgggc gaggtcacgg tgacgccgct gggcgacgag atcgtgcgcc aggcgcaaag
     2461 cgtgctggag caggcggccg agatcaagga gatcgccaag cgcggcaagg acccgctggg
     2521 cggcccgctc aagctgggcg tgatctacac catcggcccg tatctgctgc ccgacctggt
     2581 gcggcagaac attgcgctga cgccgcagat gccgctgatg ctgcaggaga acttcactgt
     2641 gcgcctgctg gagatgctgc gcaccggcga gatcgactgc gccatcctgg ccgagcc
//
LOCUS       DAOMVO010000326         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11212_length_2696_cov_16.249148, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000326 DAOMVO010000000
VERSION     DAOMVO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11212_length_2696_cov_16.249148"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /gene="tatB"
                     /locus_tag="PKV30_12260"
     CDS             complement(<1..278)
                     /gene="tatB"
                     /locus_tag="PKV30_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRL31021.1"
                     /translation="MLDLGISKMALIGAVALIVIGPEKLPRVARTVGTLLGKAQRYVS
                     DVKAEVNRAMDLDELKKMKESVEEAGREVEKGLHSTKADFEKDWSSVTA"
     gene            complement(371..601)
                     /gene="tatA"
                     /locus_tag="PKV30_12265"
     CDS             complement(371..601)
                     /gene="tatA"
                     /locus_tag="PKV30_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HRL31022.1"
                     /translation="MGSFSIWHWLIVLLIVVMIFGTKKLKNMGTDLGGAVKGFKDGMR
                     DGAADADAPAAQVGNNTAATKETIDVQAKEKT"
     gene            complement(703..1071)
                     /locus_tag="PKV30_12270"
     CDS             complement(703..1071)
                     /locus_tag="PKV30_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRL31023.1"
                     /translation="MTTSAHDPNCLFCKIVAGQIPSKKVYEDDELLAFHDIHPWAPVH
                     FLIIPKAHIPSMAQVGPEHEALMGRIMMLAPRLALEQGCNPYPAGGFRIVVNTGAEGG
                     QEVQHLHIHVMGGPRPWLKG"
     gene            complement(1110..2144)
                     /locus_tag="PKV30_12275"
     CDS             complement(1110..2144)
                     /locus_tag="PKV30_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRL31024.1"
                     /translation="MAKNGNGRAANTKTVNLALQGGGSHGAFTWGVLDALLEDGRVRF
                     EGASGTSAGAMNAVVMAHALARARANGQRGQAVNEAARAALARFWDGVGVLGSFMSGL
                     PLPAAQAVGSWFAQWLSPYQANPFGLNPLRDLIKREVDFDLLAQQRYLKVFVAATNVR
                     TGRGEIFSGARVSADAVMASACLPAMFQAVQIEGEYYWDGGYSGNPAIYPLIYETRSA
                     DVILVQINPVEAPYVPGVGAADIMERVNEITFNAPLLAEIRAIEFVSRLLAEGKLDRT
                     RYKDVLLHRVDGGAALAQFGATSKARADPAFLRRLFTLGRGAGQDWLAEHFNDIGVCS
                     SVEKVPHTRA"
     gene            complement(2263..2670)
                     /locus_tag="PKV30_12280"
     CDS             complement(2263..2670)
                     /locus_tag="PKV30_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31025.1"
                     /translation="MNNRNDIIDVDTVQSTAVARADSLKTWGWVSYILHLIVAVGAVL
                     PGAQVSIALLIIALVMDLVKKDDAAGSWQESHFKWRINSVLWAGVLYLVTAPLWLFFL
                     LPGWIAWGLISIWFLYRIVKGMVRMNAGKPITP"
BASE COUNT          450 a          916 c          856 g          474 t
ORIGIN      
        1 gcggtgacgc tgctccagtc cttctcgaaa tcggccttgg tggagtgaag gcctttttcg
       61 acctcgcggc cagcctcttc caccgattcc ttcatcttct tgagctcgtc caggtccatc
      121 gcccggttga cctcggcctt gacgtccgac acataacgct gcgccttgcc cagcagcgtg
      181 ccgacggtgc gcgccacgcg tggcagcttc tcggggccga tgacgatcag cgccacggcg
      241 ccgatgagcg ccatcttgga aatgccaagg tcaagcatct tgtggttcag ctttggatgt
      301 cgagcgcttc agcgaaaacc gcctgaatca cgctcaacgc cagacgccga acgctcaacc
      361 tgaaaaaccc tcaggttttt tccttcgcct gcacgtcgat ggtttccttg gtggccgcgg
      421 tgttgttgcc cacctgcgcg gcgggcgcgt cggcgtcggc tgcgccatcg cgcatgccgt
      481 ccttgaagcc cttgaccgcg ccgcccaggt cggtgcccat gttcttcagc ttcttggtgc
      541 cgaagatcat gacaacgatc agcaacacga tcagccaatg ccagattgaa aacgaaccca
      601 tgtcaactct ccttgcgcgc cgccattgag cgccgttgca gccattttag acgccggggg
      661 cgtcgttcag gttcccgcgc gccccgcaca cgggaaggga aatcagcctt tgagccacgg
      721 gcgcggcccg cccatgacgt ggatgtgcag gtgctgcacc tcctgcccgc cttcagcccc
      781 cgtgttcacc acgatgcgaa agccgcctgc ggggtacggg ttgcaaccct gctccagcgc
      841 cagccggggg gccagcatca tgatgcggcc catcagcgcc tcatgttccg gcccgacctg
      901 cgccatcgac gggatgtgcg ccttgggaat gatcaaaaaa tgcaccggcg cccacgggtg
      961 gatgtcgtga aaggccagca gttcgtcgtc ctcgtagacc tttttcgacg gaatctggcc
     1021 ggcgacgatc ttgcagaaca ggcaattggg gtcgtgtgcg gaagtggtca tggtgcgccg
     1081 cgccgccatc ggcgcgccaa ggagaaaaat caagcgcgag tatgcgggac tttttcgaca
     1141 ctcgagcaga cgccgatgtc gttgaagtgc tcggccagcc aatcctgccc ggcgccgcgg
     1201 ccgagcgtga acagccggcg cagaaaagcc gggtcggcgc gcgccttgct ggtggcgcca
     1261 aactgcgcca gcgcggcgcc gccgtccacg cggtgcaaca gcacgtcctt gtagcgggtg
     1321 cggtccagct tgccttcggc cagcagtcgg ctgacgaatt cgatggcgcg aatttcggcc
     1381 agcagcggcg cgttgaaggt gatctcgttc acgcgctcca tgatgtcggc ggcgccgacg
     1441 cccggcacgt agggcgcttc caccggattg atctgcacca ggatgacgtc ggcgctgcgc
     1501 gtttcgtaga tcagcgggta gatcgccggg ttgcccgagt agccgccgtc ccagtagtac
     1561 tcgccctcga tctgcaccgc ctgaaacatc gccggcaggc aggccgaggc catcaccgca
     1621 tcggccgaca cgcgggcgcc gctgaaaatc tcgccgcgcc cggtccgcac gttggtcgcg
     1681 gccacaaaca ccttcaggta gcgctgctgc gccagcaggt caaagtcgac ttcgcgcttg
     1741 atcagatcgc gcagcggatt gagcccgaac gggttggcct ggtagggcga cagccactgc
     1801 gcaaaccagc tgccgaccgc ctgcgccgcc ggcagcggca gcccgctcat gaaggagccg
     1861 agcacgccca cgccgtccca gaagcgcgcc agcgcggcgc gtgcggcctc gttcacggcc
     1921 tgcccgcgct gcccattggc gcgggcgcgc gccagggcgt gcgccatcac caccgcgttc
     1981 atggcgccgg cgctggtgcc gctggcgcct tcgaagcgca cacggccgtc ttccagcagc
     2041 gcgtccagca cgccccaggt gaatgcgccg tgcgagccgc cgccttgcag cgccaggttg
     2101 acggtcttgg tgttagctgc gcgcccgtta ccgttctttg ccatggtttt tttcagtgac
     2161 gcacaaacgg ccgcggagca ggctacgcgg gggcgccgtg gaacgggctt ggcccggcca
     2221 ctggcgccgt ccccctttta agggggaagc cgcgccagcg gctcaggggg tgatcggctt
     2281 gccggcgttc atgcgcacca tgcccttgac gatgcggtac aggaaccaga tcgagatcag
     2341 gccccaggcg atccagcccg gcagcaggaa gaacagccac agcggcgccg tcaccaggta
     2401 cagcacgccg gcccacagca ccgaattgat gcgccatttg aaatggcttt cttgccagct
     2461 gccggcggcg tcgtccttct tcaccaggtc catcaccagc gcgatgatga gcagcgcaat
     2521 gctgacctgc gcgcccggca gcaccgcgcc cacggccacg atcaggtgca ggatgtagct
     2581 gacccagccc caggtcttga gcgaatcggc gcgcgccacg gctgtggact ggacggtatc
     2641 gacgtcgatg atgtcgttgc ggttgttcat gcgccggctc ctttcgatgc ggggtc
//
LOCUS       DAOMVO010000327         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11253_length_2687_cov_14.953647, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000327 DAOMVO010000000
VERSION     DAOMVO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11253_length_2687_cov_14.953647"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKV30_12285"
     CDS             complement(<1..274)
                     /locus_tag="PKV30_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRL31026.1"
                     /translation="MRLAAAHPPPRALILAAGRGERMRPLTDTCPKPLLAVHGRPLLQ
                     WWVDALTQGGVAEIVINTAWLGEKIEDYFGRQRTPDKREQLSNQEFT"
     gene            complement(288..650)
                     /locus_tag="PKV30_12290"
     CDS             complement(288..650)
                     /locus_tag="PKV30_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL31027.1"
                     /translation="MVDDEPQVLAAWRPLLQGWGIEACCVLNGAAAWDALGRGFAPDV
                     ILCDQRLRAGEDGFELLQALLVRCPAARGAMVSGEFSSPALAQAEDEGYIVLRKPLDP
                     AILQALLARWMASAAAKS"
     gene            complement(664..1413)
                     /locus_tag="PKV30_12295"
     CDS             complement(664..1413)
                     /locus_tag="PKV30_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL31028.1"
                     /translation="MTPPAWRAAAEGLRAILVPPEMAQLSAEGRARLLRMTFRSVRYS
                     ISTIPGLSVPLVLFALYLQAGTLVARPAPVALPPLLNEIATLYRAQADRQGLRLRTWL
                     PPGDCVVQADAHLLRQALINLTHNALRYTPRGGVLLGVRRRGAHWLIEVWDTGVGVAA
                     SDQARIFAPGYRAAARAERAQRRRRGPWPRPGGGGAGGVALGRGLRHALAAGPRLVLL
                     AAPAGRGAAGRTPHGLVGGGKRAASWARRTH"
     gene            complement(1410..1655)
                     /locus_tag="PKV30_12300"
     CDS             complement(1410..1655)
                     /locus_tag="PKV30_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRL31029.1"
                     /translation="MEATELDVLVTPADLGLTERQGDILALLLDGLPNKRIAQALRLS
                     ESTVKEHVSAVLARMGAHSRVELMTRLRGQRLVRRPA"
     gene            complement(1625..2059)
                     /locus_tag="PKV30_12305"
     CDS             complement(1625..2059)
                     /locus_tag="PKV30_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL31030.1"
                     /translation="MSYGLVIDDHRLVARGVTGFLRAHPRLSDAVDAGDAEQARAILA
                     DRGMPAIVLIDFWLGNDTAQAVIAHLLVTFHGARVLVMSGDDRPGVRATAMQTGAHGF
                     VLKHESPEVVHRAVTELLDGAPGFKTSGPTPCPWKPPSSMCW"
     gene            2344..>2687
                     /locus_tag="PKV30_12310"
     CDS             2344..>2687
                     /locus_tag="PKV30_12310"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914542.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRL31031.1"
                     /translation="MPNSSLMANAIRALAMDAVQQANSGHPGAPMGMADMAVALWGRH
                     LKHNPSNPLWADRDRFVLSNGHASMLIYALLHLTGYDLPLQELKDFRQLHSKTPGHPE
                     HGLTPGVETTTGP"
BASE COUNT          430 a          992 c          909 g          356 t
ORIGIN      
        1 ccgtgaattc ctgatttgat agctgctcgc gcttgtctgg tgtgcgctgg cggccaaaat
       61 agtcttcaat cttctcgccc agccaggcgg tgttgatgac gatctcagcc acgccgccct
      121 gggtcagcgc gtcgacccac cactgcagca gcggccggcc gtgcaccgcg agcagcggct
      181 tggggcaggt gtcggtcagc gggcgcatgc gctcgccacg gccggcggcc aggatgagcg
      241 cgcggggcgg tgggtgcgcg gcggccagtc tcatgcgcgg cactttatta cgatttggcg
      301 gccgccgacg ccatccagcg cgcgagcagc gcctgcagga tcgccggatc gagcggcttg
      361 cgcagcacga tatagccttc gtcctcggcc tgtgccagtg cgggcgagct gaattcgcca
      421 ctcaccatgg cgccgcgcgc ggcggggcag cgcaccagca gcgcctgcag caactcaaag
      481 ccgtcttcgc cggcgcgcag gcgttggtcg cacaggatca cgtcgggcgc gaagccgcgg
      541 ccgagcgcgt cccacgcggc ggcgccgttc agcacgcagc acgcctcgat gccccagcct
      601 tgcagcagcg ggcgccacgc cgccagcacc tgcggctcgt cgtcgaccac caggcagcgc
      661 ccgctaatgg gtgcggcgag cccatgacgc ggcgcgcttg cctccgccga ccaggccatg
      721 gggcgtgcgg ccggcggcgc cgcggcccgc aggcgcagcc agaagcacga gccgcggccc
      781 agccgcgagc gcatgccgta gcccacgccc aagagcgacg ccgcctgcgc caccaccgcc
      841 aggccgaggc catggcccac gtcgtcgtcg ctgcgcgcgc tctgcgcggg ccgccgcacg
      901 gtagcctggc gcgaagatgc gcgcctggtc gctggcggcc acgcccacgc cggtgtccca
      961 gacctcgatc agccaatgcg cgccgcgccg ccgcacgccc aacaacacgc cgccgcgtgg
     1021 cgtgtagcgc agcgcgttgt gcgtcaggtt gatgagcgcc tggcgcagca gatgcgcgtc
     1081 ggcctgcacc acgcagtcgc cgggcggcag ccaggtgcgc agccgcaggc cctggcggtc
     1141 ggcctgcgcg cggtagagcg tggcgatctc gttcaacagc ggcggcaggg cgactggcgc
     1201 cgggcgcgcc accagggtgc ccgcctgcaa gtacagcgca aacagcacca gcggcaccga
     1261 cagacccgga atggtggaga tgctgtagcg cacgctgcga aaggtcatgc gcaacagccg
     1321 ggcgcgcccc tcggcgctca gttgcgccat ctcgggcggc accagaatgg cccgcagccc
     1381 ctcggccgcg gcgcgccagg ccggcggcgt catgccggtc gccggaccag gcgctggccg
     1441 cgcaggcggg tcatcagctc gacgcggctg tgcgcgccca tgcgcgccag cacggccgac
     1501 acatgctcct tgacggtgct ttcagacaac ctgagcgcct gggcaatgcg cttgttgggc
     1561 aaaccatcca acagcagcgc caggatgtcg ccctggcgct cggtcaggcc caggtcggcc
     1621 ggcgtcacca gcacatcgag ctcggtggct tccatgggca cggtgtcggg ccagaggtct
     1681 tgaaaccagg cgccccgtcc agcagttcgg tgacggcgcg atgaaccacc tcgggcgact
     1741 cgtgcttcag cacgaagccg tgcgcgccgg tttgcatggc ggtggcccgc acgccgggcc
     1801 gatcgtcgcc gctcatcacc agcacgcgcg cgccgtgaaa ggtgacgagc aaatgcgcga
     1861 tcaccgcctg cgccgtgtcg ttgccaagcc agaagtcgat cagcacgatg gccggcatgc
     1921 cgcggtcggc caggatcgcg cgcgcctgct cggcatcgcc ggcgtccacg gcgtcactca
     1981 gccgggggtg agcgcgcaaa aaccccgtca cgccgcgcgc gaccaggcga tgatcgtcga
     2041 tgaccagacc gtaagacatg gctgaacagg gcggtgcggc gcccagaaaa aaagcggcgg
     2101 cacggcaaag cccttgcacc ataccctacc aaggcagggt ctcgtgaagt acgccaccgg
     2161 cttgttcgcg cacgattcat gcgcgcaccg tggcggcggg tgcgcgcgac ccaggctgcc
     2221 cgtgcacggc tgtttcaggt tgcgcgccag cgcagccacc cgctgcgcaa tttgcatcgg
     2281 cgctcacgtg gggtcggtcg atagaattga gggttctgcg tagcccaccc ctgaagccgt
     2341 cccatgccga attcctccct catggccaac gccatccgcg ccctcgcgat ggacgccgtc
     2401 cagcaagcca attctggcca cccgggcgcg cccatgggca tggccgacat ggccgtcgcg
     2461 ctgtggggcc gccacctcaa gcacaacccc agcaacccgc tgtgggccga ccgcgaccgc
     2521 ttcgtgctca gcaacggcca cgcgtcgatg ctgatctacg ccctgctgca cctcacaggc
     2581 tacgacctgc cgctgcagga gctgaaagac ttccgccaac tgcacagcaa aacgcccggc
     2641 caccccgagc atggcttgac gcccggcgtg gaaaccacca ccggccc
//
LOCUS       DAOMVO010000328         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11330_length_2672_cov_14.032862, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000328 DAOMVO010000000
VERSION     DAOMVO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11330_length_2672_cov_14.032862"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKV30_12315"
     CDS             complement(<1..883)
                     /locus_tag="PKV30_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL31032.1"
                     /translation="MRVLLGFLLAACAPVTWAAHGYALWGDMKYPPGFIHFDYVNPDA
                     PKGGEIRLVSNLRLSTFDKYNPFTIRGAAPAYLSQLMFDSLLAGSLDEEGAGYGLLAE
                     DVAVAPDGLSATFKLRKNARFHNGDPVLAQDVKHSYDTLIGPHTSPAYKTLLVDVAGV
                     DVVDDRTVRYRFRQANRELPLTVGGLPVFSRKWGQGKPFNEVVMEPPIGSGPYKIGPV
                     KFGKDITYVRDTDYWAKDLNVRRGTGNFDRINVKIYKDNTARLEALKAGEFDLMAFYS
                     AGDWARRVNGKRFDTGEL"
     gene            925..1728
                     /gene="fabI"
                     /locus_tag="PKV30_12320"
     CDS             925..1728
                     /gene="fabI"
                     /locus_tag="PKV30_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRL31033.1"
                     /translation="MSNPSGFLAGKKLLITGVLSNRSIAYGIARACRNQGAELAFSYV
                     GERFKDRITDFAAEFDSKLIFDCDVGSDEQIEAMFKDLSATWPTFDGFVHAIGFAPRE
                     AIAGNFFDGLSRENYRIAHDISAYSFPGMAKAALPYLSDKAALLTLSYLGALRTIPNY
                     NTMGLAKASLEASVRYMAEALGGKGIRVNGISAGPIKTLAASGIKDFGKLLGYVAQAA
                     PLRRNVTIEDVGNVAAFLLSDLASGVTAEITYVDGGFSQTAGLSADMVG"
     gene            1799..2659
                     /locus_tag="PKV30_12325"
     CDS             1799..2659
                     /locus_tag="PKV30_12325"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HRL31034.1"
                     /translation="MKRRLLLLGCLLAVAQRAFADAPALLLAGRYRPGIDLRDWWVSE
                     KYDGVRAHWDGRQLRTRGGERIAAPAWFTAGWPALPMDGELWAGRGRFEHAQSAAARE
                     QPDDAQWRALRYMVFDLPAHPGSFDERLAALRAAVSALDHTWVQAVPQRKVAGDAELQ
                     ALLREVVRGGGEGLMLHRGASLYRAGRGDDLLKLKTHDDAEARVIAHVPGKGKHAGRL
                     GALMVETPEGRRFALGTGFTDAQRADPPPVGSWVTYRFRGLHEGSGLPRFASFLRVRS
                     DEARFRSAPR"
BASE COUNT          444 a          882 c          896 g          450 t
ORIGIN      
        1 ccagctcgcc ggtgtcgaag cgcttgccgt tcacgcgccg cgcccagtcg ccggctgagt
       61 aaaacgccat gaggtcgaac tcgcctgcct tcaatgcctc cagccgggcg gtgttgtcct
      121 tgtagatctt gacgttgatg cggtcgaagt tgccggtgcc gcggcgcacg ttcaaatcct
      181 tcgcccagta gtcggtgtcg cgcacgtagg tgatgtcctt gccgaacttc accgggccaa
      241 tcttgtacgg gccgctgccg atgggcggct ccatcaccac ttcgttgaag ggcttgccct
      301 gcccccattt gcggctgaac accggcaggc cacccacggt gagtggcagt tcgcggttgg
      361 cttgcctgaa gcggtagcgc acggtgcggt catcgaccac gtcgacaccc gccacgtcga
      421 ccagcagcgt cttgtacgcg ggcgaggtgt gcgggccgat cagggtgtcg tagctgtgct
      481 tcacatcctg cgccagcacc gggtcgccgt tgtgaaagcg cgcgttcttg cgcagcttga
      541 aggtggccga caagccgtcg ggcgccaccg ccacgtcttc ggccagcagg ccgtagccgg
      601 cgccctcttc gtccaacgag ccggccagca ggctgtcgaa catcagctgg ctcaggtacg
      661 cgggcgcagc accgcggatg gtgaaggggt tgtacttgtc aaaggttgac agccgcaggt
      721 tgctgaccag ccgaatctcg ccgcccttgg gcgcatcggg gttgacatag tcgaaatgga
      781 tgaagcccgg cgggtacttc atgtcacccc acagggcgta gccgtgggcg gcccaggtga
      841 cgggcgcgca ggccgcaagc aggaaaccaa gcaaaacacg catgcgacaa ttctgacggc
      901 atttcttaga ggagcatgaa gcacatgagc aaccccagcg gctttctggc gggcaagaaa
      961 ctgttgatca ccggcgtgct gtcgaaccgg tccatcgcct acggcatcgc gcgtgcgtgc
     1021 cgcaaccagg gcgccgagtt ggccttcagc tacgtgggtg agcgtttcaa ggaccgcatc
     1081 accgacttcg ccgccgaatt tgattcgaag ctgatcttcg actgtgacgt cggcagcgac
     1141 gagcagatcg aggcgatgtt caaggacttg tccgccacct ggccgacctt cgacggcttc
     1201 gtccacgcca tcggctttgc cccgcgcgag gccattgccg gcaacttttt cgacggcctg
     1261 agccgcgaaa actaccgcat cgcgcacgac atcagcgcct acagctttcc cggcatggcg
     1321 aaggccgccc tgccctatct gagcgacaag gccgcgctgc tgacgctgag ttatctgggc
     1381 gcgctgcgca ccatccccaa ctacaacacc atgggcctgg ccaaggcgtc gctggaagcc
     1441 agcgtgcgct acatggccga ggcgctgggc ggcaagggca ttcgcgtcaa cggcatcagc
     1501 gccggcccca tcaagacgct ggcggccagc ggcatcaagg atttcggcaa gctgctgggc
     1561 tacgtggccc aggccgcgcc gctgcgccgc aacgtgacga ttgaggacgt gggcaacgtg
     1621 gcagcctttt tgctgagcga tctggccagc ggcgtaacgg ccgagatcac ctacgtcgat
     1681 ggcggcttca gccagacggc gggcttgagt gccgacatgg tgggttgacc accaaatacg
     1741 gatcaaacag gcctccagcg cttgtccagc aagcgcaaac agctcactta ttcatagtat
     1801 gaagcgccgc ttgctgctgc tgggctgcct gctcgccgtc gcgcagcggg ccttcgccga
     1861 cgccccggcc ctgctgctgg cgggccgcta ccgccccggc atcgacctgc gcgactggtg
     1921 ggtgagcgaa aagtacgacg gcgtgcgcgc ccattgggac ggcaggcaac tgcgcacgcg
     1981 cggcggcgaa cggatcgccg cgccggcgtg gttcacggct ggctggccgg cgctgccgat
     2041 ggatggcgaa ttgtgggcgg gccgcggccg tttcgagcat gcccagtcgg ccgccgcacg
     2101 ggaacaaccc gacgacgccc agtggcgcgc cctgcgctac atggtgttcg atctgccggc
     2161 gcaccccggc agctttgacg aacggctggc cgccctgcgc gccgccgtgt cggcgctgga
     2221 ccacacatgg gtgcaggccg tgccgcaacg caaggtggcg ggcgatgccg aactgcaggc
     2281 gctgctgcgc gaggtggtgc gcggcggcgg cgaggggctg atgctgcacc gcggcgcctc
     2341 gctctaccgc gccggccggg gcgatgatct gctcaaactc aaaacccacg acgacgccga
     2401 ggcacgcgtc atcgcccatg tgccgggaaa aggcaagcac gccgggcgac tgggcgcgct
     2461 gatggtcgag acgcccgagg ggcggcgctt tgcgctgggc accggcttca ccgacgccca
     2521 gcgcgccgat ccgccacccg tcggcagttg ggtgacttat cgttttcgcg gactgcacga
     2581 aggcagcggg ctgccgcgct ttgccagctt cttgcgtgtg cgctcggatg aggcacgctt
     2641 tcgcagcgcg ccgcgttgat gccgtgcggc ac
//
LOCUS       DAOMVO010000329         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11365_length_2666_cov_13.275756, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000329 DAOMVO010000000
VERSION     DAOMVO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11365_length_2666_cov_13.275756"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKV30_12330"
     CDS             <1..1088
                     /locus_tag="PKV30_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRL31035.1"
                     /translation="FSGLFIGPTSQAPRVDEARNDQLYELEIALQEIHKARAERGEAM
                     PPWLVDRSLRNILVDITGNTHRSEFCIDKLYSPDTPTGRLGLLEMRAFEMPPHARMSI
                     AQQLLMRALVAHFWRTPYTAPAARWGTQLHDRFMLPAYIQADFDDVMADMQRAGYAFD
                     ASWFAPHLEFRFPVVGELDVLGMHVELRNALEPWHVMGEESTGSGTARYVDSSLERLQ
                     VHVTGFNPSRYAITCNGHALPLQPLGAEGGYVAGVRYKAWNPWSSLHPSIKPHTPLVF
                     DIYDTWSQRSVGGCQYHVAHPGGRNYDTFPVNAYEAESRRLARFFRMGHTSGAMEVPA
                     ATLHLPGSREFPCTLDLRRSNNTRMRQ"
     gene            1096..>2666
                     /locus_tag="PKV30_12335"
     CDS             1096..>2666
                     /locus_tag="PKV30_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRL31036.1"
                     /translation="MNQSEIPVLDALEPGPLAHAAASLPSLGPFDELRGLPSQGGQWP
                     AVLDQLRGSPMAPSWHEFFQYLGPYGCEELDRRAARLARQLRENGVTYNVYADEANLQ
                     RPWPLDLFPLLITSSDWQAIEAGVLQRVQVLETVMRDTYGERRLLKDGLLPAALVQGH
                     PDYLRAMHGVPVVGGTHLHIVAFDLARGPKGYWWVVSQRTQAPSGLGYLLENRSAVST
                     QFADAFEGMNVRRLAATYRSFIEGIKAACPAGENAHIALLTPGPYNETYFEHSYLARY
                     LGLTLVEGGDLTVRGRRLYLKTLHGLRPVHGLIKRVDDAFLDPLEMRPESRLGVPGLL
                     EAVRAGNLLVANAPGAGFLESSALLGFLPALARRLLGEELKLPALHTWWCGEPAAMRE
                     VLPELGRCVIKPTYPWSTSRGTFNAGVGPLMAPEMLASWADAIQRVPEEHTVQAYMSP
                     AQIPTWGMSGGRTGIVPRAAMLRVFALSDGAGSWQVLPGGMTRLVSESAGLATMAMGG
                     SSADTWVLGDEPLQE"
BASE COUNT          406 a          957 c          896 g          407 t
ORIGIN      
        1 tgttcagcgg cctgttcatc ggccccacca gccaggcgcc gcgcgtggac gaggcacgca
       61 acgaccagtt gtacgagctg gaaattgcgc tgcaagaaat ccacaaggcg cgcgccgaac
      121 ggggtgaagc aatgccgccg tggctggtgg atcgatcctt gcgcaacatc ctcgtcgaca
      181 tcaccggcaa cacccaccgc agcgagttct gcatcgacaa gctgtactca cccgacacgc
      241 ccaccggccg cctcggcctg ctggagatgc gcgccttcga gatgccgccg cacgcgcgca
      301 tgtcgattgc gcagcaattg ctgatgcgtg cgctggtggc ccatttctgg cgcacgccct
      361 acaccgcgcc cgccgcgcgc tggggcacgc agttgcacga ccgcttcatg ctgccggcct
      421 acatccaggc cgacttcgac gacgtgatgg ccgacatgca gcgcgctggc tacgcgttcg
      481 atgccagttg gtttgcgccg cacctcgagt ttcgctttcc ggtcgtcggc gagcttgacg
      541 tgctcggcat gcacgtcgaa ctgcgcaacg cgctggagcc ctggcacgtg atgggcgaag
      601 aaagcaccgg cagcggcacg gcgcgctatg tcgattcgtc gctggagcgc ttgcaggtgc
      661 acgtcaccgg cttcaacccc agccgctacg ccatcacctg caacggccac gcgctgccgc
      721 tgcagccgct gggcgccgag ggcggctacg tggccggcgt gcgctacaag gcgtggaacc
      781 cctggtcgtc gctgcaccca tccatcaagc cgcacacgcc gctggtgttc gacatctacg
      841 acacctggag ccagcgcagc gtgggcggct gccagtacca cgtggcgcac ccgggcgggc
      901 gcaactacga caccttcccc gtcaacgcgt atgaggccga aagccgccgg ctggcgcgct
      961 ttttccgcat ggggcacacc agcggcgcca tggaggtgcc tgccgccacg ctgcacctgc
     1021 ccggcagccg cgaattcccc tgcacgctgg acctgagacg gagcaacaac acgcggatgc
     1081 gacaatgacc gctggatgaa ccagtcggaa attcccgtcc tcgacgcgct ggagcccggg
     1141 cccctggcgc atgccgccgc gtccttgcct tcgctcggcc cctttgacga gttgcgcggc
     1201 ctgccctcgc agggcggcca gtggcccgcg gtgctggatc agttgcgcgg cagcccgatg
     1261 gcgcccagct ggcacgagtt cttccagtac ctcggcccct acggctgcga agagctggac
     1321 cgccgcgccg cacgcctggc gcgccagttg cgcgaaaacg gcgtcaccta caacgtttac
     1381 gccgacgagg ccaacctgca gcggccgtgg ccgctcgatc tgtttccgct gctgatcacg
     1441 tccagcgact ggcaagccat tgaagccggc gtgctgcagc gcgtgcaggt gcttgaaacc
     1501 gtgatgcgcg acacctacgg cgagcgccgc ctgctgaagg acggcctgct gcccgccgcg
     1561 ttggtgcaag gccacccgga ctacctgcgc gccatgcacg gcgtgccggt ggtgggcggc
     1621 acgcacctgc acatcgtcgc cttcgatctg gcgcgcggac ccaagggcta ttggtgggtg
     1681 gtgtcgcagc gcacgcaggc gccctcgggc ctgggctacc tgctggaaaa ccgctcggcc
     1741 gtctcgaccc agttcgccga tgccttcgag ggcatgaacg tgcgccgcct ggcggccact
     1801 taccgcagct tcatcgaggg catcaaggcc gcctgcccgg ccggcgagaa cgcgcacatc
     1861 gcgctgctca cgcccgggcc gtacaacgag acctacttcg agcactccta cctcgcgcgc
     1921 tacctgggcc tgacgctggt cgaaggcggc gatctgacgg tgcgcgggcg ccgcctgtac
     1981 ctgaagacgc tgcacggcct gcggccggtg cacggcctca tcaagcgggt cgacgacgcc
     2041 tttctcgacc cgctggagat gcgccccgaa tcgcgcctgg gcgtgcccgg cctgctggag
     2101 gcggtgcgcg ccggcaacct gctggtggcc aacgcgccgg gtgcgggctt tctcgaatcg
     2161 agcgcgctgc tcggctttct gcccgcgctg gcgcggcgcc tgctgggcga agagctgaag
     2221 ctgcccgccc tgcacacctg gtggtgcggc gagccggccg ccatgcgcga ggtgctgccc
     2281 gagctgggcc gctgcgtgat caagccgact tacccgtggt cgaccagccg cggcaccttc
     2341 aacgccggcg tcgggccgct gatggcgccc gagatgctgg cctcgtgggc cgacgccatc
     2401 cagcgcgtgc cggaagagca caccgtgcag gcctacatgt cgccagcgca gatccccacc
     2461 tggggcatga gcggcgggcg caccggcatc gtgccgcgcg cggccatgct gcgcgtgttc
     2521 gcgctgtccg acggcgccgg ttcgtggcag gtgctaccgg gcggcatgac gcggctggtg
     2581 agcgaaagcg ccggcctggc caccatggcg atgggcggca gcagcgccga cacctgggtg
     2641 ctgggcgacg agccgctgca agaaga
//
LOCUS       DAOMVO010000330         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11370_length_2665_cov_17.427203, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000330 DAOMVO010000000
VERSION     DAOMVO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11370_length_2665_cov_17.427203"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKV30_12340"
     CDS             complement(<1..535)
                     /locus_tag="PKV30_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRL31037.1"
                     /translation="MATATLAMPSSPVARPRRRPARRVLPGFNLTLGYTLLYLSLIVL
                     IPLSALLFKTFSMTWADFWAAVSAPRVLASFRLTFGASLVAALVNVVAGLLVAWVLVR
                     YSFPGKKIADALVDLPFALPTAVAGISLTAILAENGWIGQYLAPLGIQLAFNPKGIVI
                     ALIFIGLPFVVRTVQPVL"
     gene            complement(745..1743)
                     /locus_tag="PKV30_12345"
     CDS             complement(745..1743)
                     /locus_tag="PKV30_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL31038.1"
                     /translation="MKTSFRLAFAAVALAAASAASAQTSLLNVSYDVAREFYRDFNTA
                     FIAHYKKTTGKDIKVDQSHAGSSAQARAVADGLAADVVTMNTTTDIDFLADKGVVAKD
                     WRQKFPNGASPTTSTMLFLVRNGNPKNIKDWDDLIRPDVKVVVVNPKTGGNGRLAYLA
                     AWGQVRAKGGTDAQAAEFVSKLYKNVPALARGGRDATGMFLQRNLGDVLVTFESEVVS
                     VENEFGKGKVDAIHPSASIVAENPVAVVERTVAKKGTAAEAKAYLDYLYSPEGQEIAA
                     KHNIRPRNEAILKKHADVFKPIKLFTVDQYFGSLAEAQKVHFNDGGQFDKLYTPGK"
     gene            complement(1878..>2665)
                     /locus_tag="PKV30_12350"
     CDS             complement(1878..>2665)
                     /locus_tag="PKV30_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRL31039.1"
                     /translation="EYFCVMGQRDTDKLALQEPLGSGPIQDWLAATARELNLWLVGGT
                     LPLSCEPRDEARRSVGPPQAQAAPSGGSAAREAAERGGKCRNASLAFNPSGERVACYD
                     KIHLFKFDDGSRVYDEAAVLARGSRPTLFDLPSRDGHTWRVGMSVCYDLRFPELYRFY
                     ASAGADLLLVPSAFTYVTGQAHWELLLRARAVENLCGVVAAAQGGTHENGRRTWGHSM
                     VIDAWGQVLAQRAEGAGVALAELDFAALAARRTQLPALGHRVM"
BASE COUNT          454 a          890 c          879 g          442 t
ORIGIN      
        1 ccagcaccgg ctgcacggtg cgcaccacga agggcaggcc gatgaagatc agcgcaatca
       61 cgatgccctt ggggttgaac gcgagctgaa tgcccagcgg cgccaggtat tgcccgatcc
      121 agccgttttc cgccaggatg gcggtgagcg aaatgccggc gacggcggtg ggcagcgcga
      181 agggcaagtc gaccagcgca tcggcgatct tcttgccggg gaacgaatag cgcaccagca
      241 cccaagccac cagcaacccg gccaccacgt taaccagcgc ggcgaccagc gacgcgccaa
      301 aggtgagcct gaacgacgcc agcacgcgcg gcgcgctgac ggcggcccag aagtcggccc
      361 acgtcatcga gaaagtcttg aagagcagcg ccgacagcgg aatgagcacg atcaggctca
      421 ggtacagcag cgtgtagccg agcgtgaggt tgaagccggg cagcacgcgg cgcgcggggc
      481 ggcggcgtgg gcgcgcgacg ggtgacgagg gcatggcgag cgtggcggtg gccatatgaa
      541 tttgatagct gcttgcgctt gatggacgcg gatggcggcg gtttttctcc ctcccccgct
      601 gggggagggc aggggtgggg gctgaccacg tgcccacagc cagcggcgtt gccccccatc
      661 cccaccttcc cccagagggg gaaggagcca agtcaccctc accccaaccc tctcccgcgc
      721 gcgggagagg gagtcttcag cgccttactt gccaggcgta tacaacttgt cgaactgccc
      781 accatcgttg aaatgcacct tctgcgcctc ggccagcgaa ccgaagtact ggtccaccgt
      841 gaacagcttg ataggcttga acacgtcggc gtgtttcttc aggatcgcct cgttgcgcgg
      901 gcgaatgttg tgcttggcgg cgatttcctg gccctcgggc gagtacaggt aatccagata
      961 agccttggct tcagccgccg tacctttctt ggccaccgtg cgctccacca ccgcgaccgg
     1021 gttctccgcc acgatgctgg cgctcgggtg aatggcatcg accttgcctt tgccgaactc
     1081 gttctccacc gacaccactt cggactcaaa cgtcaccagc acgtcgccca gattgcgctg
     1141 caggaacatg cccgtggcat cgcgcccgcc gcgcgccagc gccggcacgt tcttgtagag
     1201 cttgctgacg aactcggccg cctgcgcatc ggtgccgccc ttggcgcgca cctggcccca
     1261 ggccgccaga taggccagac ggccgttgcc gccggtcttg gggttgacca cgacgacctt
     1321 cacgtcgggg cggatcaggt cgtcccagtc cttgatgttt ttggggttgc cgttgcgcac
     1381 caggaacagc atggtcgatg tggtgggcga ggcgccgttc ggaaacttct gccgccagtc
     1441 cttggcgacc acgcccttgt cggccaggaa gtcgatgtcg gtggtggtgt tcatcgtcac
     1501 cacgtcggcg gccaacccgt ccgccacggc gcgcgcctgc gcgctcgagc cggcgtgcga
     1561 ctgatcgacc ttgatgtcct tgccggtggt ctttttgtaa tgggcgatga aggcagtgtt
     1621 gaagtccctg tagaactcgc gcgcgacgtc gtaggagacg ttgagcaggc tggtctgcgc
     1681 gctggcagcg ctggcggcgg ccagagccac agcggcgaag gcgaggcgga aagaggtttt
     1741 catggcacgt caaaaaacaa ggccgacgaa gtgtcggctg tcccctaaag tttggcgcgg
     1801 cgcccttaaa actcaaacga cttttttgtt gtttctttat gcgtgaaaac ggataaagcg
     1861 gcacacgggc ccagccgtca catcacccgg tgccccagcg ccggcaactg cgtccgccgc
     1921 gccgccagcg ccgcaaagtc caactctgcc aaagccacgc ccgcgccttc ggcccgctgc
     1981 gccagcacct ggccccaagc atcgatcacc atgctgtggc cccaggtgcg gcggccgttc
     2041 tcgtgcgtgc cgccttgcgc ggcggcgacc acgccgcaca ggttttcaac cgcgcgggcg
     2101 cgcagcagca gttcccaatg cgcctggccg gtgacgtaag tgaaggcgct gggcaccagc
     2161 agcaggtcgg cgcctgcgga cgcgtaaaag cggtacagct cgggaaagcg caggtcgtag
     2221 cacacgctca tgcccacgcg ccaggtgtgg ccgtcgcgcg aaggcagatc gaacagcgtc
     2281 gggcggctgc cgcgcgccag cacggccgcc tcgtcgtaca cgcggctgcc gtcgtcgaac
     2341 ttgaacaggt ggatcttgtc gtagcaggcc acgcgctcgc cgctcgggtt gaacgccagg
     2401 ctggcattgc gacacttgcc cccacgctct gccgcttcgc gtgctgcgct gccccccgag
     2461 ggggctgcct gcgcttgggg cggcccgacg cttcggcgcg cttcatcacg cggctcgcac
     2521 gacaggggca gcgtgccgcc gacgagccac aggtttagct cgcgcgccgt ggcggccagc
     2581 cagtcctgga tcgggccact gcccagcggc tcctgcaacg ccagcttgtc ggtgtcgcgc
     2641 tggcccatga cgcagaagta ttcgg
//
LOCUS       DAOMVO010000331         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11400_length_2660_cov_17.770825, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000331 DAOMVO010000000
VERSION     DAOMVO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11400_length_2660_cov_17.770825"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKV30_12355"
     CDS             <1..375
                     /locus_tag="PKV30_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL31040.1"
                     /translation="RTLLLGSALQGVALLMFLPVDGLVPLYVVSALFGLFQGGIVPSY
                     AIIIREHFPAREAGARTGAVIMATLVGMALGGWMSGWVFDMTGSYRAAFINGIGWNLL
                     NLSIASWLYWRTRGPSPRLALR"
     gene            complement(454..651)
                     /locus_tag="PKV30_12360"
     CDS             complement(454..651)
                     /locus_tag="PKV30_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31041.1"
                     /translation="MRLSTVMAVLLIGTVLYIVMAVIRAIVIGRMEGMQDEALDYRRK
                     AVRAGIFGAVVLLVAYVMDWW"
     gene            complement(688..1152)
                     /locus_tag="PKV30_12365"
     CDS             complement(688..1152)
                     /locus_tag="PKV30_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31042.1"
                     /translation="MTTIELFHLRRVRDKPRAAALLAAHASLTAEAALAAVHQAVGGG
                     KPQITVADEDGARRLIAALAGTGFIARRAAAGDFDAAQHAGHALATVLPRCAPGAADA
                     AGAALLAGDWAEALALALQHLQMHRPADDADRVLVEHAAIDTGLVRGVPGRV"
     gene            complement(1149..2201)
                     /gene="murB"
                     /locus_tag="PKV30_12370"
     CDS             complement(1149..2201)
                     /gene="murB"
                     /locus_tag="PKV30_12370"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRL31043.1"
                     /translation="MIVERNVPLQPLNTFGIAARADTLVRVRGEDDVRDLLRHALLDQ
                     APKLILGGGSNVVLTGDVKGVVLKVEVPGRRVVRETGRHVIVEAGAGENWHDLVTWTL
                     DQGLPGLENLALIPGTVGASPVQNIGAYGLELQDRFDSLDAIDFTTGDVFSLNAAQCG
                     FGYRDSVFKHPAAQVGDFGLAGRSLILRVRFALPKDWQPVLGYLDIERRMQEEGVAHP
                     TARQLYDWIVAIRSAKLPDPAVIGNAGSFFKNPTVTPEQCADIIGRDPKVVHYPMPDG
                     SVKLAAGWLIDACGWKGKHVGNAAVYEKQALVLVNRGGRENPATGGEVMTLAKAIQTS
                     VYERFGIRLEPEPVVV"
     gene            2245..>2660
                     /locus_tag="PKV30_12375"
     CDS             2245..>2660
                     /locus_tag="PKV30_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRL31044.1"
                     /translation="MPSFDTVCDPDMVEVKNAVENAAKEIGTRFDFKGTSAAIELKDK
                     DITLLGDAEFQLQQVEDILRNKLTKRNVDVRFLDTGKVEKMGGDKVKQVVKVKSGIAG
                     DDAKKIQKLIKESKLKVQAAIQGDSVRVTGAKRDDL"
BASE COUNT          440 a          887 c          898 g          435 t
ORIGIN      
        1 cgcacgctgc tgctgggctc ggcactgcag ggcgtggcgc tgttgatgtt tctgccggtg
       61 gacgggctgg tgccgctgta cgtggtgtcg gcactgttcg ggctgttcca gggcggcatc
      121 gtgccgtcgt acgcgatcat catccgcgag cactttccgg cgcgcgaagc cggcgcgcgc
      181 accggcgccg tcatcatggc cacgctggtg ggcatggcgc tgggcggctg gatgagcggc
      241 tgggttttcg acatgacggg cagctaccgc gcggccttca tcaacggcat cggctggaac
      301 ctgctgaacc tgagcatcgc cagctggctg tactggcgca cgcgcgggcc gtcgccgcgc
      361 ctggcgctgc gctagggcgc cgattcagct gcttttattt tcatagctgt tcgcgcttgt
      421 ctggcgtgcg ccagcggcca tttttcttca aattcaccac cagtccatga cgtaggcgac
      481 cagcagcacg acggcgccga agatgcccgc gcgcacggcc tttcggcggt agtcgagcgc
      541 ctcgtcctgc atgccttcca tgcggccgat gacgatggcg cggatgaccg ccatgacgat
      601 gtacaagacc gtgccgatca gcagcacggc catcacggtc gataaacgca tgtcgtctgc
      661 cctttcagtt cgttgaagcc tgcgggttca cacccgcccc ggcacgccgc gcaccaggcc
      721 ggtgtcgatg gccgcgtgtt ccaccagcac gcggtcagca tcgtcggccg ggcggtgcat
      781 ctgcaggtgc tgcaaggcga gcgcaagcgc ctcggcccag tcaccggcca gcagcgcagc
      841 gccggccgca tcggcggcgc cgggggcgca gcggggtagc accgtcgcca gcgcgtggcc
      901 ggcgtgctga gcggcatcga aatcgccagc ggccgcgcgc cgggcgatga agccggtgcc
      961 cgccagcgcc gcaatcaggc gccgcgcccc gtcttcgtcc gccacggtga tctgcggctt
     1021 gccgccgccc acggcctggt gcacggcggc caaggccgcc tcggccgtca aactggcgtg
     1081 cgcggccagc aaggcggcgg cgcggggctt gtcgcgcacg cggcgcaggt gaaacagctc
     1141 gatggtggtc acaccaccac cggctcgggc tccagccgga tgccaaagcg ctcgtacacg
     1201 ctggtctgga tggccttggc cagcgtcata acttcgccgc cggtggccgg gttctcgcgc
     1261 ccgccgcggt tgaccagcac cagcgcctgc ttctcgtaca ccgccgcgtt gccgacgtgc
     1321 ttgcccttcc agccgcaggc gtcgatcagc cagccggccg ccagcttcac gctgccgtcg
     1381 ggcatggggt aatgaaccac tttcgggtcg cgaccgatga tgtcggcgca ctgctcgggc
     1441 gtgacggtgg ggttcttgaa aaagctgccg gcgttgccga tgacggccgg gtcgggcagc
     1501 ttggcgctgc gaatcgccac gatccagtca tagagctggc gcgcggtggg gtgcgccacg
     1561 ccctcttcct gcatgcggcg ctcgatgtcc agatagccca gcaccggctg ccagtccttc
     1621 ggtaaggcaa aacgcacgcg caggatgagc gagcggccgg ccagcccgaa atcgcccacc
     1681 tgcgcggccg ggtgcttgaa caccgaatcg cggtagccga agccgcactg cgcggcgttc
     1741 aggctgaaca cgtcgccggt cgtgaagtcg atggcgtcga gcgagtcaaa gcggtcttgc
     1801 agctccagcc cgtaggcgcc gatgttctgc accggcgatg cgcccacggt gccggggatc
     1861 agtgccaggt tttccagccc cggcaggcct tgatccaaag tccaggtgac taagtcgtgc
     1921 cagttctcgc ccgcgccagc ttcgacgatg acgtggcgac ccgtctcgcg caccacgcgg
     1981 cggccgggca cttcgacctt cagcaccacg cctttcacgt cgcccgtcag caccacgttg
     2041 ctgccgccgc ccaggatgag tttgggcgcc tgatcgagca gcgcgtggcg cagcaggtcg
     2101 cgcacatcgt cttcgccgcg cacgcgtacc agcgtgtcgg cgcgcgcggc gatgccgaag
     2161 gtgttcagcg gttgcagggg gacgttgcgc tccacgatca tgggacaatt gtcgcattcg
     2221 gtttttgctt tgaaggaacg gcccatgccc tcattcgaca cggtttgcga ccccgacatg
     2281 gtggaagtga agaacgcggt ggaaaacgcc gccaaggaga tcggcacgcg cttcgatttc
     2341 aagggcacga gcgccgccat cgagctgaaa gacaaggaca tcaccctgct gggcgacgcc
     2401 gaatttcagc tgcagcaggt cgaagacatc ctgcgcaaca agctgaccaa gcgcaacgtg
     2461 gacgtgcgct ttctcgacac cggcaaggtc gagaaaatgg gcggagacaa ggtcaagcag
     2521 gtcgtcaagg tgaaaagcgg cattgccggt gacgacgcca agaagatcca gaagctgatc
     2581 aaggaaagca agctcaaggt gcaggccgcc atccagggcg acagcgtgcg cgtgaccggc
     2641 gccaagcgcg acgacctgca
//
LOCUS       DAOMVO010000332         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11405_length_2659_cov_14.020353, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000332 DAOMVO010000000
VERSION     DAOMVO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11405_length_2659_cov_14.020353"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /gene="glmM"
                     /locus_tag="PKV30_12380"
     CDS             <1..977
                     /gene="glmM"
                     /locus_tag="PKV30_12380"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747734.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRL31045.1"
                     /translation="AVEAMMAEAPQWVDSAHLGRARRLDDAAGRYIEFCKSTFSSHLS
                     LRGMKIVVDAANGAAYQIAPKVFHELGAEVISIGCAPDGLNINDGVGATHPEALARTV
                     LEQRADYGIALDGDADRLQMVDATGRLFNGDELLYLLAADRLAHREAKGQARGPELAG
                     VVGTLMTNMAVELGIRRLGYEFVRAKVGDRYVLEELHRRGWILGGEGSGHLLMLERHS
                     TGDGLVSALQVLRACAASGRTVAEQLADVALFPQTLINVRLQPGQDWQSNQRLKDETQ
                     AVEAELADAGRVLIRASGTEPLVRVMVEAQDGAVAQASAERLASAITE"
     gene            1060..1821
                     /locus_tag="PKV30_12385"
     CDS             1060..1821
                     /locus_tag="PKV30_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HRL31046.1"
                     /translation="MTLKRDPLASQEVNLSEADGIRYLHLGTEWVQGSMRINAPFDID
                     LEYVQRMMAWLLFVAPHSVTGRHAMQLGLGAGAITKFCYKKLKMTCTAVEINPRVADV
                     CRAWLRLPPEDERLRVVIGDAAREIRQPEWLGTVDALQVDLYDHEAAAPVLDSPDFYA
                     DCRHLLTEDGCMTVNLFGRSASFERSVESIASAFGPRAVWQFKPTREGNAVVLAQRTP
                     SRPKRDELQARADEIEARWDLPARKWLKVFKPVEK"
     gene            2032..>2659
                     /locus_tag="PKV30_12390"
     CDS             2032..>2659
                     /locus_tag="PKV30_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HRL31047.1"
                     /translation="MADAYRGPVQWRQLVDWLREDGVITAVEADRTIARCSQAESAQP
                     ALVRLAAVGMQRASDGQAMDVEELTQHLARRAGLPYLRIDPLRVDAGKVSDTMSATYA
                     ERHKVLPVQVTAAEVVVATSEPFIDDWVAEVERQSRRSVRRVVANPAEIKRFTAEFFA
                     LAKSVRAAIKSGGAAGATSFEQLVELGKTGRQLDANDQGVIQVVDWLWQ"
BASE COUNT          455 a          876 c          932 g          396 t
ORIGIN      
        1 tggccgtcga agccatgatg gccgaggcac cgcagtgggt cgattcggcg cacctgggcc
       61 gcgcgcgccg gctcgacgac gctgccggcc gctacatcga attctgcaag agcaccttca
      121 gcagccattt aagcctgcgc ggcatgaaga tcgtggtgga tgcggccaat ggcgcggcct
      181 accagatcgc gcccaaggtg ttccatgagc tgggcgccga agtgatttcg atcggctgcg
      241 cgcccgacgg cctgaacatc aacgacggcg tcggcgccac gcaccctgaa gcgctggcgc
      301 gcacggtgct ggagcagcgc gccgactacg gcatcgcgct agacggcgac gccgatcgcc
      361 tgcagatggt cgatgccacc ggccggctgt tcaacggcga cgaattgctc tacctgctgg
      421 ccgccgaccg cctcgcgcac cgcgaggcca agggccaggc gcgcggaccc gaactcgccg
      481 gcgtggtcgg cacgctgatg accaacatgg cggtcgagct gggcatccga cgcctgggct
      541 acgagttcgt gcgcgccaag gtgggcgacc gctacgtgct ggaagagttg caccggcgcg
      601 gctggattct gggcggcgag ggctcgggcc acctgctgat gctggagcgg cacagtacgg
      661 gcgacgggct ggtgtcggcg ctgcaggtgc tgcgcgcctg cgccgccagc ggtcgtacgg
      721 tggccgagca gctggccgat gtcgcgctgt ttcctcaaac gctgatcaac gtgcgcctgc
      781 agccggggca ggactggcaa agcaatcaac gcctgaagga cgaaacccag gcggttgaag
      841 ccgagctggc ggacgctggc cgcgtgctga tccgcgccag cggcaccgaa ccgctggtgc
      901 gcgtgatggt cgaggcgcag gatggcgccg tggcgcaagc cagcgccgag cggctggcca
      961 gcgccatcac ggaatgatga taaaaaaggc cgccagcgct tgatggacaa gcgcaagcag
     1021 ctatgaaaat tgaagtgccc aacgcctcgc cataatttca tcaccttgaa acgcgatccc
     1081 ctggcctcgc aggaggtcaa tctgtccgaa gccgacggca tccgctacct gcacctgggc
     1141 accgagtggg tgcagggcag catgcgcatc aacgcgccgt tcgatatcga tcttgagtat
     1201 gtgcagcgca tgatggcgtg gctgctgttt gttgcgcccc attcggtgac gggccgccac
     1261 gccatgcagc tgggtctggg cgcgggcgcc atcaccaagt tctgctacaa aaaactcaag
     1321 atgacctgca ccgcggtcga aatcaacccg cgcgtggccg acgtgtgccg cgcctggctg
     1381 cgcttgccgc ccgaggacga gcgccttcgc gtggtgatcg gcgacgccgc gcgtgaaatt
     1441 cgccagcccg aatggctggg caccgtggat gcgctgcagg tcgatctgta cgaccacgag
     1501 gccgctgcgc cggtgctaga cagcccggat ttctacgccg attgccgcca tctgttgacc
     1561 gaagatggct gcatgacggt caacctgttc ggccgctcgg ccagcttcga gcgcagcgtg
     1621 gagagcatcg ccagcgcctt cggcccgcgc gccgtatggc agttcaagcc cacgcgcgag
     1681 ggcaacgccg tcgtgctggc gcagcgcacg ccgagccgcc ccaagcgcga tgagttgcag
     1741 gcgcgcgccg acgagatcga ggcgcgctgg gatctgcccg cacgcaagtg gctgaaggtc
     1801 ttcaaacctg tagagaaatg atccccagga atgggctccc cctgagtcgc ctacggcgcc
     1861 gtcccccagg gcacaacgcc ggtggccggt gcaagcccgg ccacggcgtt cccgcgtggc
     1921 ctgctccgcg gccattcggg caactcagcg tggggctgcc cgtgacggac aggctgtgcg
     1981 cgatggagat ttgacgtgaa acacaagaca aaacccgccc cgctcacgcc catggccgac
     2041 gcctaccgcg gccccgtgca atggcgccag ttggtcgact ggctgcgcga agacggggtc
     2101 atcaccgccg tcgaggccga ccgcaccatc gcccgctgct cgcaggccga gagcgcgcag
     2161 ccggcgctgg tgcggctggc ggcggtcggc atgcagcgcg ccagcgatgg ccaggcgatg
     2221 gacgtggagg agctgacgca gcatctggcg cggcgcgccg gcctgccata tttgcgcatc
     2281 gatccattgc gcgtcgatgc cggcaaggtg tcggacacca tgagcgccac ctatgccgag
     2341 cgtcacaagg tgctgccggt gcaggtcacg gcggccgagg tggtggtggc caccagcgag
     2401 cccttcatcg acgactgggt ggccgaggtc gagcgccagt cgcgccgcag cgtgcgccgc
     2461 gtggtggcca acccggccga gatcaagcgt ttcacggccg agttctttgc gctggccaag
     2521 tcggtgcgcg cggcgatcaa gagcggcggc gcggcgggcg cgaccagctt cgagcaactg
     2581 gtggagctgg gcaagaccgg ccgccagctc gacgccaacg accagggcgt gatccaggtg
     2641 gtcgactggc tgtggcagt
//
LOCUS       DAOMVO010000333         2635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11549_length_2635_cov_16.270930, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000333 DAOMVO010000000
VERSION     DAOMVO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11549_length_2635_cov_16.270930"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..468)
                     /locus_tag="PKV30_12395"
     CDS             complement(49..468)
                     /locus_tag="PKV30_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31048.1"
                     /translation="MKRWSAWGLLWATGAGAQGTSPTLVYPDELLPTRMVLCQREVGG
                     AERERDRSAKLRTCLARRLEGERIVERNCKREAGSVNGASARAQAQRACERQALGVPS
                     SELPRRPPPPPKPKVEPGLGTEPSAAAPARMPAAGDQ"
     gene            644..2146
                     /locus_tag="PKV30_12400"
     CDS             644..2146
                     /locus_tag="PKV30_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRL31049.1"
                     /translation="MDAATAESAPLTLQKPELQAQVVAALQRVLPPESLLWTPEQTTP
                     YECDGLTAYRQRPLAVALPASYEQIQQVLRTCHALGVPVVARGAGTGLSGGAMPHERG
                     VTLSLAKFNRILKVDPLARTALVQCGVRNLAISEAVAAHGLYYAPDPSSQIACTIGGN
                     VAENSGGVHCLKYGLTLHNVLKVKGFTVEGEPIEFGGDALDAPGYDLLALAVGSEGML
                     AVMTEVTVKLTPKPQLALCIMASFADVRHAGDAVAAIIAQGIIPAGLEMMDKPMTAAV
                     EDFVHAGYDLDAAAILLCESDGTPHEVAEEVARMEAVLRQAGATRLAVCADEAERLKF
                     WSGRKNAFPASGRISPDYMCMDSTIPRKRLADILLSIQEMERKYGLRCCNVFHAGDGN
                     LHPLILFDANDPDQLHRAELFGADILETSVAMGGTVTGEHGVGVEKLNSMCVQFSAEE
                     NAQMLGVKRAFDPQGLLNPGKVIPTLARCAEYGKMLVRGGALPHPELERF"
     gene            complement(2391..>2635)
                     /locus_tag="PKV30_12405"
     CDS             complement(2391..>2635)
                     /locus_tag="PKV30_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31050.1"
                     /translation="CKSCTPRRVSSRVISLLTAEALMQMRVLQQAGAAGANLASSLNI
                     SAFNLGNALGAWVGGLVLSHGFGLLALGGAAAEPEI"
BASE COUNT          437 a          904 c          874 g          420 t
ORIGIN      
        1 cgctgcatgc gggctttttt tgcgcttggc ggcaatgaag caacgccgct actggtctcc
       61 cgccgccggc atgcgcgcgg gtgcggcggc gctcggctca gtgcccagcc cgggctccac
      121 cttaggcttg ggcggtgggg gcggccgacg cggcaattca gacgacggca cgccgagcgc
      181 ctgccgttcg caggcgcgct gggcctgcgc gcgggcgctg gcgccgttca cgctgccggc
      241 ctcgcgcttg cagttgcgct cgacgatgcg ttcgccctcc agccggcgcg ccaggcaggt
      301 gcgcagcttg gcgctgcgat cacgctcgcg ctcggcgccg cccacctcgc gctggcacag
      361 caccatgcgc gtgggcagca gctcgtcggg gtagaccaag gtcggcgacg tgccctgcgc
      421 gcccgcgccc gtggcccata aaagacccca ggccgaccat cgtttcatcg cggttgaatg
      481 ctgcatctcg tttttctgtg tggaaagcgg gctcgctcgc ggcgccggcg cccccgacac
      541 cggcagccga attgtcgccg cgcccccccg acgcccccgt gacggcgcgc cacggcgccc
      601 gcccatgccc tatagtgggc cgatcgccat ccgaagcccc gccatggacg ccgccaccgc
      661 cgaatcggcc cctttgacac tacaaaaacc agagctgcaa gcgcaggtgg tagctgcgct
      721 gcagcgtgtt ttgccgcctg aatcgctgtt gtggacgcct gagcagacca cgccctacga
      781 atgcgacggc ctgaccgcct accgccagcg cccgctggcc gtggccttgc ccgccagtta
      841 cgaacagatt caacaagtgt tgcgaacctg tcacgcgctg ggcgtgccgg tggtggcgcg
      901 cggcgccggc accggcctct cgggcggcgc catgccgcac gagcgtgggg tgacgctgtc
      961 gctggccaaa ttcaatcgca tcctgaaagt cgacccgctg gcgcgcacgg cgctggtgca
     1021 gtgcggcgtg cgcaacctgg ccatcagcga ggcggtggcc gcgcacggcc tgtactacgc
     1081 gcccgacccg tcgagccaga tcgcctgcac catcggtggc aacgtggccg aaaactccgg
     1141 cggcgtgcac tgcctgaaat acggtctgac gctgcacaac gtgctgaaag tgaagggctt
     1201 cacggtggaa ggcgagccca tcgagttcgg tggcgacgcg ctcgacgcgc ccggctacga
     1261 cctgctggcg ctggccgtcg gcagcgaggg catgctggcg gtgatgaccg aggtcaccgt
     1321 caagctcacg cccaagccgc agctggcgct gtgcatcatg gccagctttg ccgacgtgcg
     1381 gcacgcgggc gacgcggtgg cggccatcat tgcgcagggc atcatcccgg ccgggctgga
     1441 gatgatggac aagcccatga ccgccgccgt cgaggatttc gtgcacgccg gctacgacct
     1501 ggacgcagcc gccatcctgc tgtgcgaaag cgacggcacg ccgcacgagg tggccgagga
     1561 agtggcgcgc atggaggccg tgctgcgcca ggccggcgcc acgcgcctgg cggtgtgcgc
     1621 cgacgaggcc gagcggctga aattttggag cgggcgcaag aacgcctttc ccgccagcgg
     1681 gcgcatcagc cccgactaca tgtgcatgga ttcgaccatc ccgcgcaagc gcctggccga
     1741 tatcctgcta tcgattcagg agatggagcg caaatacggc ctgcgctgct gcaacgtttt
     1801 tcacgccggc gacggcaacc tgcatccgct gatcctgttc gatgcaaatg acccggatca
     1861 gctgcaccgg gccgaattgt ttggcgccga cattctggaa accagcgtgg cgatgggcgg
     1921 cacggtgacg ggcgaacatg gcgtgggggt ggaaaagctc aactcgatgt gcgtgcagtt
     1981 cagcgccgaa gaaaacgcgc agatgctggg cgtcaagcgc gccttcgatc cgcaggggct
     2041 gctcaacccc ggcaaggtga tccccacgct ggcgcgctgc gccgagtacg gcaagatgct
     2101 ggtgcgcggc ggggcgctgc cgcatccgga gctggagcgc ttctgaagcg cagcgctcag
     2161 ttgatattgt tttgatagct gtttgcgctg gtctggcaag cgctggcggc caatttggct
     2221 ctgaatcacg atcacagcgc caccacaatc gtaggctggg tagagcacca acctaccaaa
     2281 aggtgtgaat ccgaaagatc aaccgccaag cccctgcagc agcaccgaat tcacttctat
     2341 ttttatagct gcttgcgctt gactcgcaag cgctgaaggc cgatttgacg ctaaatctct
     2401 ggctccgccg ccgccccacc cagcgccagc agcccgaagc cgtgcgacag caccagcccg
     2461 ccgacccagg cgcccagcgc gttgcccagg ttgaaggccg agatgttcag gctcgacgcc
     2521 aagttggcgc ccgccgcgcc cgcctgttgc agcacgcgca tctgcatcag cgcctcggcc
     2581 gtcagcaagc tgatgacacg gctggaaacg cgcctgggcg tgcagctttt gcacc
//
LOCUS       DAOMVO010000334         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11648_length_2617_cov_9.814598, whole genome shotgun sequence.
ACCESSION   DAOMVO010000334 DAOMVO010000000
VERSION     DAOMVO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11648_length_2617_cov_9.814598"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PKV30_12410"
     CDS             <1..1554
                     /locus_tag="PKV30_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRL31051.1"
                     /translation="YLHSMMMAGIVASHETTSNGIANALKILLQNPEVWRELCDDPSL
                     IPSAVEECIRYNGSVAAWRRIATRDATVGGVHIPAGSKLLIVMSSANHDERHFADADR
                     VDIRRDNASDHLTFGYGSHQCMGKNLARLELQVFLEEFTKRLPHVRLSEQRFDYVPNT
                     SFRGPKHLWVEWDPAHNPERANPALLQTELPVRLGEPSKQTVTRPMVVERVTRITDDA
                     VLLRLVAANGQPLPKWQPGAHIDIECGDTELSRQYSLCGDPADAGAFEIAVLRDAASR
                     GGSTWVHDQLKPGDRVRIRGPRNHFHFDEAAKKAIFIAGGIGITPIAAMAHRARLLGM
                     DYEIHYSGRSRTLMPLLDALIATHDERLHVYAKDEGQRADLTALLATPQPGVRIYACG
                     PERMLNALPACTAHWPDDALRIEHFQTEPPTLDPAKEHAFEIELKDSGITLPVRADQT
                     ILSALKAANIDGQSDCCEGLCGSCEVRVLGGAVDHRDLVLTRGERDAQDRMMVCCSRA
                     AGAKLVLEL"
     gene            complement(1642..2466)
                     /locus_tag="PKV30_12415"
     CDS             complement(1642..2466)
                     /locus_tag="PKV30_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRL31052.1"
                     /translation="MTIRKRPAPAPAQSVAEPRRRRVQSAEMGMGILKSLAALGGAAS
                     LTAVAQAVGESTAKVHRYMASLVEQDLVAQDAATQRYFLGQAAIHIGLAALRQCEPIR
                     AGEPALARIQSELGVTCFIAVMGNQGPTILRIEEPALPVVVNVRAGSVLPLLWSATGQ
                     AFLAFADEAGMDARSLQEWKQAAPAQRALLPEKAPIAAMRERVRAQGCASVRDLLAPG
                     ISAVAAPIRRHTGQVAAVLTALGASGGFDSAPEGRIAARVRQEADAISAALGFVDG"
BASE COUNT          443 a          929 c          839 g          406 t
ORIGIN      
        1 tacctgcact cgatgatgat ggccggcatc gtcgcctcgc acgagaccac gtccaacggc
       61 atcgccaacg cgctgaagat cctgctgcaa aaccccgagg tgtggcgcga actgtgcgac
      121 gaccccagcc tgatccccag cgcggtggaa gagtgcatcc gctacaacgg ctcggtcgcc
      181 gcgtggcggc gcatcgccac gcgcgacgcc acggtgggcg gcgtgcacat ccccgccggc
      241 agcaaactgc tgatcgtgat gtcgtcggcc aaccacgacg agcgccactt tgccgatgcc
      301 gaccgcgtcg acatccgccg cgacaacgcg agcgaccacc ttaccttcgg ctacggctcg
      361 caccagtgca tgggcaagaa cctggcgcgg ctggaattgc aggtgtttct ggaagagttc
      421 accaagcgcc tgccgcacgt gcgtttgagc gagcagcgct ttgactatgt gcccaacacg
      481 tccttccgtg gccccaagca tttgtgggtg gagtgggacc cggcgcacaa ccccgagcgc
      541 gccaaccccg cgctgttgca aaccgagttg cccgtgcgcc tgggcgagcc gtccaagcag
      601 acggtgacgc ggcccatggt cgtcgagcgc gtcacgcgca tcaccgacga cgcggtgctg
      661 ctgcggctgg tggccgccaa cggccagccg ctgcccaaat ggcagcccgg cgcgcacatc
      721 gacatcgagt gtggtgacac ggagctctcg cgccagtatt cgctgtgtgg cgatcccgcc
      781 gatgccggcg cattcgagat cgccgtgctg cgcgacgcgg ccagccgggg cggctcgacc
      841 tgggtgcacg atcaactgaa gccaggcgac cgcgtgcgca ttcgcggccc gcgcaaccac
      901 tttcactttg acgaagcggc gaagaaagcc atcttcatcg ctggcggcat cggcatcaca
      961 cccatcgccg ccatggcgca ccgcgcgcgg ctgctgggca tggattacga aatccactac
     1021 agcggccgca gccgcacgct catgccgttg ctggatgcac tcatcgccac gcacgacgag
     1081 cgcctgcatg tctacgccaa ggacgaaggc cagcgcgccg acctgacggc gctgctggcc
     1141 acgccgcaac ccggcgtgcg catctacgcc tgcggccccg agcgcatgct gaacgcgctg
     1201 cccgcctgca ccgcgcactg gcccgacgac gcgctgcgca tcgaacactt ccagaccgag
     1261 ccgcccacgc tggacccggc caaggaacac gcgtttgaga tcgagctgaa agactccggc
     1321 atcacgctgc cggtgcgtgc cgaccagacc atcctctcgg ccctgaaagc cgccaacatc
     1381 gatgggcaga gcgattgctg cgagggcctg tgcggctcgt gcgaagtgcg cgtgctgggc
     1441 ggcgccgtcg atcaccgcga cctggtgctg acgcgcggcg agcgcgacgc gcaagaccgc
     1501 atgatggtgt gctgctcgcg cgcggcgggc gcgaagctgg tgctggagct ttgacaagcg
     1561 cccgcggaat atcaaaaatg atagctgctt gcgcttgcct atcaagcgct ggcgcttttt
     1621 ttactgaaaa accgaccgct gtcagccgtc cacgaagccc agcgccgcgc tgatcgcatc
     1681 cgcctcttgc cgcacccgcg cggcgatgcg gccctcgggc gctgaatcga aaccccccga
     1741 cgcacccagc gccgtcagca ctgccgccac ctggccggta tggcggcgga tcggcgccgc
     1801 caccgcgctg atccccggcg ccagcagatc gcgcacgctg gcgcagcctt gtgcgcgcac
     1861 gcgctcgcgc atcgcggcaa tcggggcttt ttcgggcagc agcgcacgct gggcgggcgc
     1921 ggcctgcttc cattcttgta gcgaccgggc atccatgccg gcttcgtcgg caaacgccag
     1981 aaaagcctgg ccggtggccg accacagcaa cggcaatacc gatcccgcgc gcacgttcac
     2041 caccaccggc agcgccggct cctcgatgcg cagaatcgtc gggccctggt tgcccatgac
     2101 ggcgatgaag caggtcacgc ccagctcgga ctggatgcgc gccagcgccg gctcgccggc
     2161 acggatcggc tcgcattggc gcagcgccgc cagcccgatg tggatggccg cctggcccag
     2221 aaagtagcgc tgcgtggccg cgtcctgcgc aaccagatcc tgctcgacca ggctggccat
     2281 gtagcggtgc accttggccg tgctttcgcc caccgcctgc gccaccgccg tcaggctggc
     2341 ggcgccgccc aacgcggcca ggcttttcag gatgcccatg cccatttcgg ccgattgcac
     2401 gcggcggcga cgcggttcgg ctacggattg ggcgggggcc ggggcaggtc gttttcggat
     2461 cgtcatgaag gcaagcgtat gtgtagaaat ccggagcaat cattgcgcaa tgggaagtag
     2521 tattacgcgt tttgacgcac atcaatcgtt catcatgaag tcattgatct ccgccgcgct
     2581 ggtcgcgctt ccgctggcct tgggcgccag cgccgct
//
LOCUS       DAOMVO010000335         2611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11677_length_2611_cov_15.420970, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000335 DAOMVO010000000
VERSION     DAOMVO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11677_length_2611_cov_15.420970"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..617
                     /locus_tag="PKV30_12420"
     CDS             81..617
                     /locus_tag="PKV30_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31053.1"
                     /translation="MKHSALLALVVAAALVGCGGSDDDDNITPAPAPAPAPSVPGATV
                     QALYDYAGNWVEHDERCEWNASAAQYDKEVDVITRTADGVFQRQETTNYYADGQCAGA
                     VTRSVVTSVEYWYVVGHGQLADGTRVDQIHETAQAGGAVRELCVAFVQDGKLFKECND
                     AGRAFPTAIDRTEWDVKQ"
     gene            complement(920..>2611)
                     /locus_tag="PKV30_12425"
     CDS             complement(920..>2611)
                     /locus_tag="PKV30_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRL31054.1"
                     /translation="AIACLDVMVALGVRPDNVYACDSKGLIYEGRPGGFDESKARYAQ
                     PDTGLRTLADVVKGADVFLGCSAPGVLTGEMVKTMADQPVILALANPEPEIRPEDAKA
                     ARPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEAMKLACVHEIANLAKAE
                     TSAEVAAAYAGKDLQFGPDYIIPTPFDPRLITRIAPAVAKAAVESGVAERPITDMEAY
                     RTSLERFVYQTGMVMRPVYDAAKAVPHDRKRVAYAEGEDERVLRAAQIAIDDRLAMPI
                     LIGRPAVIEQRIQKAGLRMRLGADVQCVNPEDDPRFRQYWEAYHRLMARNGVTVEAAK
                     AAVRRSSTIIAALMVHLGDADAMLCGALGRFDQHLEHIHDIIGVEPGAPGYATVNALL
                     LQDRTLFVADTYVNEDPSAELLAAIAKMAADEVHNFGLPPKVAFVSHSNFGSSKRPSA
                     LKMRRARDLFRAQHPSIMCDGEMHGDSALSSTLRERAVADSTLDGEANLLVCPSLDAA
                     NILFNVLKITVGHGVTIGPILLGTAAPAHVLTASATVRRVVNMTALAVAEAESKR"
BASE COUNT          408 a          839 c          943 g          421 t
ORIGIN      
        1 atcgaggcgt tcaagcagta agctggcgtg ccaaagctgc tacgatggcg ttctgtcagt
       61 ttttcaagga ggaacgtccc atgaaacact ctgcgttgtt ggccctggtg gtagccgcgg
      121 ccttggtcgg ctgcggtggc agcgatgacg acgacaacat cacgcccgca ccggcaccag
      181 cccctgcgcc ttctgtacca ggtgctaccg tgcaggcgct gtacgactac gctggcaatt
      241 gggtagagca tgacgagcgc tgcgagtgga acgcctcggc cgcccagtat gacaaggagg
      301 ttgacgtcat cacccgcacg gcggacggtg tgttccagcg ccaggaaacc accaactact
      361 acgccgatgg gcaatgcgcg ggtgcggtga cgcgcagcgt ggtcacctcg gtggaatact
      421 ggtacgtggt gggtcacggg cagctggccg acggcacgcg tgtggatcag atccacgaaa
      481 ccgcccaggc gggcggcgcg gtgcgagagc tgtgcgtggc cttcgtgcag gacggcaagc
      541 tgttcaagga gtgcaacgac gctggccgcg ctttccccac ggcgattgac cgcactgaat
      601 gggatgtgaa gcagtaagcc tgcgcgcctc aaaaaagccc gccccaacaa aggggcgggc
      661 tttttttgtg ttcagaacag cgatggaggc gcgaaagagc gctgactagc cgccgcgtgc
      721 ccccatccct gccttccccc agcgggggaa ggagcaaggg cagggccgcg aagccggccc
      781 accgaaaggc cgcttaagca ggccaaaaac cagggcaccc gtggccgggg tccccccggc
      841 cagtgggtgc gtcccccctc ccgaagggcg aagcccgcag agagaggggg gaaggcgccg
      901 aaggcgcctc aggggggtgt caccgtttac tttctgcctc cgccaccgcc agcgccgtca
      961 tgttcaccac gcgccgcacc gtggccgatg ccgtcagcac gtgggccggc gcggcggtgc
     1021 cgagcaggat ggggccgatg gtgacgccgt ggcccacggt gatcttcagc acgttgaaca
     1081 atatgttggc cgcgtccagg ctggggcaga ccagcaggtt ggcttcgccg tcgagcgtgc
     1141 tgtcggccac ggcgcgttcg cgcagggtcg acgacagcgc gctgtcgccg tgcatttcgc
     1201 cgtcgcacat gatggacggg tgctgcgcgc ggaacaggtc gcgcgcgcgc cgcatcttca
     1261 gcgccgaggg gcgcttggac gagccgaagt tggagtgcga tacgaaggcc accttgggcg
     1321 gcaggccgaa gttgtgcacc tcgtcggcgg ccatcttggc gatggcggcc agcagctcgg
     1381 cgctggggtc ttcgttgacg taggtgtcgg ccacgaacag cgtgcggtcc tgcagcagca
     1441 gcgcgttcac ggtggcgtag ccgggcgcgc cgggctccac gccgatgatg tcgtggatgt
     1501 gctccaggtg ctggtcgaag cggccgagcg cgccgcacag cattgcgtcg gcgtcgccca
     1561 ggtgcaccat cagcgcggcg atgatggtgg aggagcgccg caccgcggcc ttggccgcct
     1621 cgacggtgac gccgttgcgc gccatcaggc ggtggtaggc ctcccagtac tggcgaaagc
     1681 gcgggtcgtc ctcggggttc acgcactgca cgtcggcgcc caggcgcatg cgcaggccgg
     1741 ctttctggat gcgctgctcg atcaccgccg ggcggccgat caggatcggc atggccagcc
     1801 ggtcgtcgat ggcgatctgc gccgcgcgca gcacgcgctc gtcctcgccc tcggcatagg
     1861 ccacgcgctt gcggtcgtgc ggcacggcct tggcggcgtc gtacacgggg cgcatcacca
     1921 tgccggtctg gtagacgaag cgctccaggc tggtgcggta ggcctccatg tcggtgatgg
     1981 ggcgttccgc cacgcccgat tcgaccgccg ccttggccac cgccggcgcg atgcgcgtga
     2041 tcaggcgcgg gtcgaacggc gtcgggatga tgtagtcggg gccgaactgc aagtccttac
     2101 cggcatacgc cgccgccact tcggcgctgg tctcggcctt ggccaggttg gcgatttcat
     2161 gcacgcaggc cagcttcatg gcctcggtga tcttggtggc gccgcagtcc agcgcgccac
     2221 ggaagatgta cgggaagcac aggacgttgt tgacctggtt cgggtagtcc gagcggccgg
     2281 tggcgatgat gcagtcgggc cgcgcggcct tggcgtcctc ggggcggatt tcgggctcgg
     2341 ggttggccag cgccaggatc accggctggt cggccatggt cttgaccatc tcgcccgtca
     2401 gcacgccggg cgcagagcag ccgaggaaca cgtcggcgcc cttcaccacg tcggccagcg
     2461 tgcgcaggcc ggtgtcgggc tgggcgtagc gcgccttcga ttcgtcgaag ccgccggggc
     2521 gcccttcgta gatcaggccc ttggagtcgc aggcgtacac gttgtcgggc cgcacgccca
     2581 gcgccaccat cacgtccagg caggcgatgg c
//
LOCUS       DAOMVO010000336         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11737_length_2601_cov_19.887667, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000336 DAOMVO010000000
VERSION     DAOMVO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_2601_cov_19.887667"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2601
                     /locus_tag="PKV30_12430"
     CDS             <1..>2601
                     /locus_tag="PKV30_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRL31055.1"
                     /translation="TEIHDYLRLLFARAGTPYCPDHDLPLTVQTVSQMVDAVLALPED
                     TKIMILAPVAREKKGEFAELFEQMQAQGYVRFRVDGQVLAVEDLPQLKKAEKHDLDVV
                     IDRLKVRPDMKARLADSFEAALRLADGRAIALEMDSGQEHLFNSRFACPVCGYSIAEL
                     EPRLFSFNAPAGACPSCDGLGTTEVFDAERVVAFPSLSLASGAIKGWDRRNGYYFALI
                     ESLAKHYKFSVDTPFEQLPEAVRHAILHGSGDEEIKFSYTFESGASAGRKVNKKHPFE
                     GILPNMARRYRETDSPIVREELARYRGTQPCTACGGTRLRKEARHVFVGEAGQRRAIY
                     QISHITLGEAKAYFDALQLQGAKAEIADKVVREIGNRLKFLNDVGLNYLSLERSAETL
                     SGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNDRLIGTLQHLRDLGNSVLVVE
                     HDEDMMRAADHVIDMGPGAGVHGGRVMAQGTFAQVAAAPDSVTGQYLRGTRAIAIPKR
                     RAAPAGLRVDYEMDSCSRLTDKRQSPEMLENAAPEGVLRIVRASGHNLKNVSVDVPVG
                     LLTCVTGVSGSGKSTLVNDTLYAAAARQIHRAHDEPAPHVAIEGLEQFDKVIAVDQSP
                     IGRTPRSNPATYTGLFTPIRELMAEVATAKERGYGPGRFSFNVPQSSGGGRCEACQGD
                     GVVKVEMHFLPDVYVPCDVCHGQRYNRETLEVLWKGKNIAQILELTVEDAAAFFQDVP
                     TIARKLQTLLDVGLSYIRLGQAATTLSGGEAQRVKLAQELSKRDTGRTLYILDEPTTG
                     LHFADIDLLLKVLHQLRDAGNTIVIIEHNLDVIKTADWLIDMGPEGGAGGGTVVGVGT
                     PEELAANPAS"
BASE COUNT          465 a          881 c          888 g          367 t
ORIGIN      
        1 tgaccgagat tcacgactac ctgcgcctgc tgtttgcgcg cgccggcacg ccgtattgcc
       61 ccgatcacga cctgccgctc accgtgcaga ccgtgagcca gatggtcgac gcggtgctgg
      121 cgctgcccga ggacacgaag atcatgatcc tcgcgccggt ggcgcgcgag aagaagggcg
      181 agttcgccga gctgtttgag cagatgcagg cgcagggcta cgtgcgcttt cgcgtcgacg
      241 gccaggtgct ggcggtggaa gatttgccgc agctgaaaaa ggccgagaag cacgacctcg
      301 acgtcgtcat cgaccgcctc aaggtgcgcc ccgacatgaa ggcgcgcctg gccgacagct
      361 tcgaggccgc gttgcgcctg gccgatggcc gcgccatcgc gctggagatg gatagcggcc
      421 aggagcacct gttcaacagc aggttcgcct gcccggtgtg cggttattcg attgccgaac
      481 tggagccgcg cctgttctcc ttcaacgccc cggccggcgc ctgccccagc tgcgacggcc
      541 tgggcaccac cgaggtgttc gacgccgagc gcgtggtggc cttcccatcg ctcagcctgg
      601 ccagcggcgc catcaagggc tgggaccggc gcaacggcta ctacttcgcg ctgatcgaat
      661 cgctggccaa gcattacaag ttcagcgtcg acacgccgtt cgagcagctg cccgaagcgg
      721 tgcgccacgc catcctgcac ggctccggcg acgaggagat caagttcagc tacaccttcg
      781 agagcggcgc atcggccggc cgcaaggtga acaagaagca cccgttcgag ggcatcctgc
      841 ccaacatggc gcggcgctac cgcgagaccg attcgcccat cgtccgcgag gaactggccc
      901 gctaccgcgg cacgcagccc tgcacggcct gcggcggcac gcgcctgcgc aaggaggcac
      961 gccacgtgtt cgtgggcgag gccgggcagc gccgcgcgat ctaccagatc agccacatca
     1021 cgctgggcga ggccaaggcg tatttcgacg cgctgcaact gcagggcgcc aaggccgaga
     1081 tcgccgacaa ggtggtgcgc gagatcggca accgcctcaa gttcttgaac gacgtcggcc
     1141 tgaactacct gagcctggaa cgcagcgccg agacgctgag cggcggcgag gcgcagcgca
     1201 tccgcctggc cagccagatc ggcagcggcc tgacgggcgt gatgtacgtg ctggacgagc
     1261 ccagcatcgg cctgcaccag cgcgacaacg accgcctgat cggcacgctg cagcacctgc
     1321 gcgacctggg caacagcgtg ctggtggtcg agcacgacga agacatgatg cgcgcggccg
     1381 accacgtgat cgacatgggc cccggcgccg gcgtgcacgg cgggcgcgtg atggcgcagg
     1441 gcacgttcgc gcaggtggcc gcggcgcccg attcggtgac cggccagtac ctgcgcggca
     1501 cgcgcgccat cgccatccca aagcgccgcg cggcgccggc ggggctgcgt gtggactatg
     1561 aaatggatag ctgctcgcgc ttgacagaca agcgccagag ccctgaaatg cttgaaaacg
     1621 cggcacccga gggcgtgctg cgcatcgtcc gcgccagcgg gcacaacctg aagaatgtca
     1681 gcgtcgatgt gccggtcggg ctgctgacct gcgtgacggg cgtgtcgggc tcgggcaagt
     1741 cgacgctggt caacgacacg ctgtacgccg ccgccgcgcg gcagatccat cgcgcgcacg
     1801 acgagccggc gccgcacgtg gccatcgaag ggctggaaca gttcgacaag gtcattgccg
     1861 tcgatcagtc gcccataggc cgcacgccgc gcagcaaccc ggccacctac acggggctgt
     1921 tcacgccgat ccgcgagctg atggccgaag tcgccaccgc caaggagcgc ggctatggcc
     1981 ccgggcgctt cagcttcaac gtgccgcaat ccagcggcgg cggccgctgc gaagcctgcc
     2041 agggtgacgg cgtggtgaag gttgagatgc actttttgcc cgatgtctac gtgccctgcg
     2101 acgtctgcca cggccagcgc tacaaccgcg agacgctgga agtgctgtgg aagggcaaga
     2161 acattgcgca gattctggag ctgacggtgg aggacgcggc ggccttcttc caggatgttc
     2221 caacaatagc ccgcaagctg cagacgctgc tggacgtggg cctgtcgtac atccgcctgg
     2281 gccaggcggc caccacgctc tcgggcggcg aggcgcagcg cgtgaagctg gcgcaggagc
     2341 tgagcaaacg cgacacgggc cgcacgctct acatcctgga cgagcccacc accggcctgc
     2401 actttgccga catcgacctg ctgctcaagg tgctgcacca gctgcgcgat gcgggcaaca
     2461 ccatcgtcat catcgagcac aacctcgacg tgatcaaaac cgccgactgg ctcatcgaca
     2521 tgggccccga aggcggtgcg ggcggcggca ccgtggtggg cgtgggcacg ccggaggagc
     2581 tggcggccaa cccggccagc c
//
LOCUS       DAOMVO010000337         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11822_length_2588_cov_11.046190, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000337 DAOMVO010000000
VERSION     DAOMVO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11822_length_2588_cov_11.046190"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /gene="gluQRS"
                     /locus_tag="PKV30_12435"
     CDS             complement(<1..734)
                     /gene="gluQRS"
                     /locus_tag="PKV30_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRL31056.1"
                     /translation="MYVGRFAPSPTGPLHAGSLVAALASWLDARAHGGQWLVRIEDVD
                     VPRCVPGADALILQQLAACGLHPDAPPAWQSRRGGRYQAALDQLLAAGRAYPCGCSRK
                     DIEAGQAARGVVRQRGAELPYPGTCRPEHGGLGNRAARAWRFHTGIFESFEPPALVQQ
                     ARAAIDSEVNSHATPWHDRRLGPQQQDVAQVVGDFVLRRADGLWAYQLAVVVDDADQG
                     VTHVVRGEDLADNTPRQILLQRALDLP"
     gene            complement(847..1914)
                     /locus_tag="PKV30_12440"
     CDS             complement(847..1914)
                     /locus_tag="PKV30_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL31057.1"
                     /translation="MTRTRPRLSSPRRIAVIGAGIAGLTAARTLQRAGHDVQVFEAAP
                     VPGGRVATQDSPFGGFDSGAQYFTVRDARFAKALEATAADIVRRWSANAVRVLDEHGR
                     VVEAALPAREAHWVAVPAMARLPERWAAPLATAGALHLATRVTHIARDALAPSRWQLH
                     TEGAEPQAVQAGFDAVLWALPPSNVRDALVKSERLAALVEPLSKVDVAPCWSLMLAYP
                     NAAQPGLQTLGPQWSAARSTHHRVAWLARESSKPGREQIERWTLQASAAWSREHENDS
                     AERVTAKLLKAFAEVAGIRATPAWSDARLWRQAQTLKPLGRPYVWDAARGIGLCGDWC
                     IGHRVEDAFVSGLELALAVHG"
     gene            2059..2478
                     /locus_tag="PKV30_12445"
     CDS             2059..2478
                     /locus_tag="PKV30_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRL31058.1"
                     /translation="MNALQNPLALIGRLLLAYFFIPAGIGKIGGFAGTVGYIASKGLP
                     LPEVGAVLAIIVEVGVGLMLLLGFKTRWAALILMVFTLAASVFFHNYWALPEAQAAMQ
                     KLMFNKNLGVAGGLLAFAAFGAGAFSLDGLSQRGGRR"
BASE COUNT          407 a          885 c          896 g          400 t
ORIGIN      
        1 ggtaaatcca gcgcgcgctg cagcagaatc tggcgcggcg tgttgtcggc caggtcttcg
       61 ccgcgcacga cgtgggtcac gccctggtcg gcgtcatcga ccaccaccgc caactgatac
      121 gcccacaggc cgtcggcgcg gcgcagcacg aagtcgccca cgacctgcgc cacgtcctgc
      181 tgctgcgggc cgaggcggcg gtcatgccat ggggttgcgt ggctgttcac ttcactatcg
      241 atagctgctc gcgcttgctg cacaagcgct ggaggctcga atgactcaaa aattccggtg
      301 tgaaagcgcc atgcgcgcgc cgcccggttg cccagaccgc cgtgttcggg ccggcaagtg
      361 ccggggtagg gcagctcggc cccgcgttgc cgcacgacgc cgcgcgcggc ctgccccgct
      421 tcaatgtcct tgcgcgagca gccgcagggg taggcgcgcc cggcagccag cagttgatcc
      481 agcgccgcct gatagcgtcc gccgcgccgc gactgccatg ccggcggcgc atccgggtgc
      541 agcccgcagg cggccaattg ctgcaggatc agcgcgtcgg cacccggcac gcagcgcggc
      601 acatcgacgt cttcgatgcg caccagccat tgcccgccgt gggcgcgcgc gtcgagccaa
      661 ctcgccagcg ccgccaccag cgagccggcg tgcagcggcc cggtgggcga gggggcgaag
      721 cggccgacgt acatgaagca atgacggaat gactcaagcc gcgccgcggc ttcaatgacg
      781 aaatgacggg ggttcagtca tggcgcgcca gcgccgtgat cgaaggcgcc agccgagctc
      841 atttcgtcat ccgtgcacgg ccagcgccag ctccagcccc gagacaaagg cgtcctccac
      901 ccggtggcca atgcaccagt cgccgcacag gccgatgccg cgcgcggcgt cccacacgta
      961 cggtcggccc agcggcttga gcgtctgcgc ttgccgccac agccgcgcat cgctccaggc
     1021 cggcgtggcg cggatgccgg cgacctcggc aaaggctttc aacagcttgg cggtgacgcg
     1081 ctcggcactg tcgttctcat gttcgcgcga ccaggcggcg ctggcttgca gcgtccagcg
     1141 ctcgatctgc tcgcgcccgg gtttggacga ttcgcgcgcc agccaggcca cgcggtggtg
     1201 cgtgctgcgc gcggcactcc actgcgggcc cagggtttgc aggccgggct gcgcggcgtt
     1261 cggataggcc agcatcaagc tccagcacgg cgccacgtcg accttcgaaa gcggctcgac
     1321 caaggcagca agtcgctctg atttcacgag cgcgtcgcgc acgttggatg ggggcaaagc
     1381 ccataaaacg gcatcaaaac ccgcctgcac ggcctgcggc tcggcgcctt cggtgtgcag
     1441 ctgccagcgc gacggtgcga gtgcgtcgcg cgcgatgtgc gtcacgcgcg tggccagatg
     1501 cagcgccccc gcggttgcca gcggggcggc ccagcgctcg ggcagtcgcg ccatggcggg
     1561 cacggcgacc cagtgcgcct cgcgcgcggg cagcgcggcc tcgaccacgc ggccgtgttc
     1621 gtccagcacg cgcacggcgt tggcgctcca gcggcgcacg atgtcggcag cggtcgcttc
     1681 aagtgccttg gcaaagcgcg cgtcgcgcac ggtgaagtac tgcgcgccgc tgtcgaagcc
     1741 accaaacggc gagtcctgcg tggccacgcg cccgcccggc accggggcgg cctcgaacac
     1801 ctgcacgtcg tggcccgcgc gctgcagcgt gcgcgcagcg gtcaggccgg cgatgccggc
     1861 gccgatgacg gcgatgcggc ggggagagga aaggcgcgga cgggttcgtg tcatgcccgg
     1921 cactctacac gctggcgcgt gcctcaagat gcgggcagca gcgcttgaac cgcctgaagg
     1981 atgccctcga acgcctgcag ccgcgcacgc ggaggtcttg taaggtcacg tctcagacca
     2041 tttgaagcac cagctcccat gaacgcattg caaaaccccc tcgccttgat cggccgcctg
     2101 ctgctggcct atttcttcat cccggccggc atcggaaaga tcgggggttt tgccggcacc
     2161 gtgggctaca tcgcatcgaa aggcttgccg ctgccggagg tgggtgcggt gctggccatc
     2221 atcgtggaag tcggcgtggg gctgatgctg ctgctgggct tcaagacgcg ctgggcggca
     2281 ctgatcctga tggtgttcac gctggccgcg agcgtgtttt ttcacaacta ctgggcgctg
     2341 cccgaggcgc aggcggccat gcagaaactg atgttcaaca agaacctggg tgtggcaggc
     2401 ggcctgctgg cgtttgccgc ctttggcgca ggtgcgttca gcctggatgg gctcagccaa
     2461 cgcggcggcc gacgttgaat tctggatcaa aatggccgtt ggcgcttgtt catcaagcgc
     2521 gggaagctat aaataacgta gcgcgccaac gattttcgcg ctgcatgaaa aaagccccgg
     2581 catgccgg
//
LOCUS       DAOMVO010000338         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11833_length_2586_cov_14.463058, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000338 DAOMVO010000000
VERSION     DAOMVO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11833_length_2586_cov_14.463058"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PKV30_12450"
     CDS             complement(<1..787)
                     /locus_tag="PKV30_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HRL31059.1"
                     /translation="MKNRFDLILIAAGACLACATAANAGQRCDTAKPTPRVIERGLNL
                     AQRTAAALDAEHRQHGTRVVLLARAGQDLSKYGQHWSHVGWAYKTAEGPWRVVHKLNE
                     CGTDQSLITRQGLGEFFLDDLWRYEAAWAPPPPELQDALWKLLQDNRRAPALHQRRYS
                     MVSYAWGTRYQQSNQWAIETLALAAEPQVQRREQAQAWLRLKDYRAATLRIGALTRLG
                     GRMTAGNVAFDDHPNAQRFADRIETVTADSVLDWLQRLSAGGAA"
     gene            complement(798..1274)
                     /locus_tag="PKV30_12455"
     CDS             complement(798..1274)
                     /locus_tag="PKV30_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31060.1"
                     /translation="MMSNETLMNHLRRTTAAVLCASAMLVAAGSAQAQSEVSLGLSML
                     PVASVVGTASVAGAAASAVVAVPAALLVSGAVLTVKAVEVSARGTVFVLERASDGAVA
                     SVELGAAAAGSVALGVGASVVTTMIASGVILSAAGEVLCFIPNAIGRALLYNEKIL"
     gene            complement(1408..1869)
                     /locus_tag="PKV30_12460"
     CDS             complement(1408..1869)
                     /locus_tag="PKV30_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HRL31061.1"
                     /translation="MQTPFIRRDTKAWQLQVWTSFGIAVTLCATGLAFLPGQDLDRAF
                     MFMGYLFCLCTSFVLSKFVRDGESAQGAARVADTPMFRFVVWGGFGLAMALTAWGLIR
                     MDINDTYKVFLGVSWLYLITGAFTLAKMLRDRQEADLLEAEWRGRREARGE"
     gene            complement(2007..2465)
                     /locus_tag="PKV30_12465"
     CDS             complement(2007..2465)
                     /locus_tag="PKV30_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31062.1"
                     /translation="MSAAAPLWLLLIQERITLIQQAPGAQNAADLTLPIGTATLRLHG
                     DPQTPLQIEHAIESVEDVVMNVRAHLGAAARLATRDAALHALARHADAAGGWLGIDAV
                     EQLFSRLAARAAGRPAAQDALPVDGASAARLVILRELMHHGALSGLQLID"
BASE COUNT          410 a          893 c          888 g          395 t
ORIGIN      
        1 gcgctgcgcc gccggccgac aggcgctgca gccagtcgag caccgaatcg gccgtcaccg
       61 tctcgatgcg gtcggcaaag cgctgcgcgt tggggtggtc gtcaaacgcc acgttgcccg
      121 ccgtcatgcg gccgcccagg cgcgtcagcg cgccgatgcg cagcgtcgcg gcacggtagt
      181 ccttcaggcg caaccaggcc tgcgcctgct cgcgccgctg cacctgcggt tcggccgcca
      241 gcgccagcgt ttcgatggcc cactggttgc tttgctggta gcgcgtaccc caggcgtagc
      301 tgaccatgct gtagcgacgc tggtgcagcg ccggtgcgcg gcggttgtct tgcagcaatt
      361 tccacagcgc gtcttgcagc tcgggcggcg gcggcgccca ggcggcttcg tagcgccaca
      421 gatcgtccag aaagaattcg cccaagccct ggcgcgtgat gagcgactgg tcggtgccgc
      481 attcgttgag cttgtgcacc acgcgccacg ggccttcggc cgtcttgtag gcccagccga
      541 cgtgcgacca gtgctggccg tacttgctca agtcttgtcc ggcgcgtgcc agcagcacca
      601 cgcgcgtgcc gtgctggcgg tgctcggcgt ccagtgcggc ggcggtgcgt tgggccaggt
      661 tcaggccgcg ctcgatgacg cgcggcgtgg gcttggcggt gtcgcagcgt tgaccggcgt
      721 ttgcggccgt ggcgcacgcc agacaagcgc cagcagctat caaaatcaaa tcgaatcgat
      781 ttttcatggc gtccccctca caagattttt tcgttgtaaa gcagcgcgcg cccgatggcg
      841 ttggggatga aacacagcac ctcacccgcc gccgacagga tgacgccgct ggcgatcatg
      901 gtggtcacca cgctggcgcc cacgcccagc gccacgctgc ctgccgccgc cgcgcccagc
      961 tcgacgctgg ccactgcgcc gtcgctggcg cgctccagca cgaagacggt gccgcgcgcc
     1021 gagacctcga ccgccttcac cgtcagcacc gcgccgctga ccagcagcgc ggccggcacg
     1081 gctaccacgg cgctggccgc cgcgcccgcc accgacgccg tgccgacgac cgaagccacc
     1141 ggcagcatcg acaggccaag ggagacttcg ctctgcgcct gggcgctgcc ggccgccacc
     1201 agcatggcgc tggcgcacaa cacggcggcg gtggtgcggc gcaggtggtt catgagtgtt
     1261 tcgttggaca tgatggactc ctcaggaagg gatggggatg accgcgctgc gcgcgatgat
     1321 ctggctgcgg gcgcagcgtc gatgacgaaa tgacggtgtt cgtcatggcg cgcagcgccg
     1381 tcatcgaagc gcagcgcggt catggcgtca ttcaccacgc gcctcacgcc ggccgcgcca
     1441 ttcggcttcc agaagatcgg cttcctgccg atcgcgcagc atcttggcca gcgtgaaggc
     1501 gccggtgatc aggtacagcc agctcacgcc gaggaaaacc ttgtaggtgt cgttgatgtc
     1561 catgcggatc aggccccagg cggtgagcgc catcgccagc ccgaaaccgc cccagacaac
     1621 gaagcggaac atgggcgtgt cggccacgcg cgccgcgccc tgcgccgatt cgccgtcgcg
     1681 cacgaacttc gacaacacga agctagtgca caggcagaac aagtagccca tgaacatgaa
     1741 ggcgcggtcg aggtcctgcc ccggcaaaaa cgccaggccg gtggcgcaca aggtcacggc
     1801 gatgccgaac gaagtccaca cctgcaactg ccaggccttg gtgtcgcggc ggataaaagg
     1861 ggtttgcatg agcgttccat gggtggggtt gaacatggaa cgcagtctgg ccgcgcggcg
     1921 ggcccaagtg gcgttgaaat gcgctgggtg gcgattgggc ggctttcagg tatcggcggg
     1981 tgataccgat accgacgcgc gcgccgtcag tcgatcaact gaaggccgct caaagccccg
     2041 tggtgcatca actcgcgcag gatcaccagg cgcgccgcgc tggcgccatc gaccggcagc
     2101 gcgtcctgcg cggcggggcg gccggcggcg cgcgcggcca ggcggctgaa gagttgctcg
     2161 acggcgtcga tgcccagcca gccacctgcg gcatcggcgt ggcgcgccaa cgcgtgcagc
     2221 gccgcgtcgc gcgtggcgag ccgcgcggcg gcgcccaggt gcgcgcgcac gttcatcacg
     2281 acgtcttcaa ccgactcgat cgcgtgctcg atctgcagag gcgtctgcgg atcgccgtgc
     2341 aggcgcagcg tggcggtgcc gatgggcaac gtcaggtcag cagcattctg tgcccctggt
     2401 gcttgctgga tgagcgtgat gcgctcctga atcagaagca gccacagcgg cgcggcagcg
     2461 ctcatgccgc gctgcgcgcc cagtcccaca cataggtctc gccgcgcgcc atgcgctgcg
     2521 cgtgctcggc cacgacctgc tcgaagcgcg ccatgtcggc atccggcacg ccctccagcc
     2581 gcacgc
//
LOCUS       DAOMVO010000339         2578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11876_length_2578_cov_15.287356, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000339 DAOMVO010000000
VERSION     DAOMVO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11876_length_2578_cov_15.287356"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKV30_12470"
     CDS             <1..568
                     /locus_tag="PKV30_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRL31063.1"
                     /translation="DGQVVSLPVQRNLHRGGILAVTEVYEGNVPPALARQAQAATIDI
                     ANELSYVGVLCVEYFVLQDGRLIVNEMAPRPHNSGHYTVDACDASQFDLQVRTLAGLP
                     LPPPRQHSAAIMLNLLGDLWFDAACAPRPLPWADVLALPGAHLHLYGKTEARPGRKMG
                     HLTFTGPDAASVRERALRAAALLGIEAF"
     gene            570..1562
                     /locus_tag="PKV30_12475"
     CDS             570..1562
                     /locus_tag="PKV30_12475"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRL31064.1"
                     /translation="MVLDGNAPESIARAADALAAGQLIGLPTETVYGLAADAGSDAAV
                     RGIFAAKGRPAGHPLIVHVASAAQVSDFADQVPVFAQKLIDAFWPGPLTLILPRRAGV
                     AEAAAGGHATIGVRCPAHPVAQALLAACAARGVRGLAAPSANRFGRISPTTAQHVAQE
                     FGDELLVLDGGPCAVGIESTIVDCTRGAPVLLRPGQLARADIERVAGQHLLSKEELLA
                     PDPQAPGTLLAHYAPRAKLRLMDVRELRAALDMLGADAKNIAVWARAPLRSASRALLL
                     RRMPDDADAAAQQLFAVLRGFDDAGVKLIWVEAPPPEPTWDGVRDRLQRAAASA"
     gene            1632..2540
                     /locus_tag="PKV30_12480"
     CDS             1632..2540
                     /locus_tag="PKV30_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRL31065.1"
                     /translation="MNASWIGRAARTLIGASAVALLAACGSGSVVSDLTPKRFITVGD
                     GFMDVGQAGYRYTVNDGSNIWVQDLAAHYQQTITSANSGGWGYAQGGARVAAADAGGA
                     PSVTAQIDAVLARTTFNANDDVVVVGGGIADVVAAVGATGFTDQTKKDVQAAGKALGE
                     QIKRLVDAGAAHVLVVGVYNLGNTPWAAGQNQKSALTDLSVDFNTGVQLEIVNLGKNV
                     LFVDPALLHNLMYNKPENYQFDNGRDAVCTTPDATTCNTSTLVAGADHNKWLYADGLY
                     LTPEASRRFGSDRYSESVYYKFKNRW"
BASE COUNT          404 a          892 c          891 g          391 t
ORIGIN      
        1 cgacggccag gtcgtcagcc tgccggtgca gcgcaacctg caccgcggcg gcatcctcgc
       61 cgtgaccgag gtttatgaag gtaacgtgcc gccagcgctg gccagacaag cgcaagcagc
      121 tacgattgat atagcaaacg agttgagcta cgtcggcgtg ctgtgcgtcg aatacttcgt
      181 gctgcaggac ggccgcctga tcgtcaacga aatggcgccg cgcccgcaca acagcggcca
      241 ctacacggtc gacgcgtgcg acgcctcgca gttcgatctg caggtgcgca cgttggccgg
      301 cctgcccctg ccgccgccgc gccagcacag cgccgccatc atgctcaacc tgctgggcga
      361 tctgtggttt gacgctgcgt gcgccccgcg cccactgccg tgggccgatg tgctggcgct
      421 gcccggcgcg catctgcacc tgtacggcaa gaccgaagcg cggccggggc gcaagatggg
      481 gcacctgact ttcaccgggc ccgatgccgc gagcgtgcgc gagcgtgcgc tgcgcgcggc
      541 ggcgttgctg gggattgagg cgttttgaga tggtgctgga tggcaacgcg cccgaatcca
      601 tcgcccgcgc cgccgatgcg ctggccgccg gccagttgat cggcctgccg accgaaaccg
      661 tctacggcct ggccgccgac gccggcagcg acgcggcggt gcgcggcatc tttgccgcca
      721 aggggcgccc ggccggtcac ccgctgattg tgcacgtggc cagtgcggcg caagtgagcg
      781 actttgcgga tcaggtgccg gtttttgcgc aaaagctcat cgacgccttc tggcccggcc
      841 cgctgacgct gatcctgccg cgccgcgcgg gcgtggccga ggcggcggcg ggcgggcacg
      901 ccaccatcgg tgtgcgttgc ccggcgcacc cggtggcgca agcgctgttg gcggcctgcg
      961 ccgcgcgcgg cgtgcgcggg ctggcggcgc ccagcgccaa ccgctttggc cgcatcagcc
     1021 cgaccacggc gcagcacgtg gcgcaggaat ttggcgacga actgctggtg ctcgatggcg
     1081 ggccgtgcgc ggtgggcatc gaatcgacca tcgttgattg cacgcgcggc gcgccggtgc
     1141 tgctgcgccc ggggcagctc gcgcgggccg acatcgagcg cgtggcgggt caacacctgc
     1201 tatccaaaga agagctgctc gcgcccgatc cacaagcgcc aggcacgctt ttggcccatt
     1261 acgcaccgcg cgccaagctg cgcctgatgg acgtgcgcga gctgcgtgcc gcgctcgaca
     1321 tgctgggtgc cgacgcgaaa aacatcgccg tctgggcgcg cgcgccgctg cgctcggcca
     1381 gccgtgcgct gctgctgcgc cgcatgccgg acgatgcgga tgccgctgcg cagcagttgt
     1441 ttgccgtgct gcgtggcttt gacgacgccg gcgtcaaact catctgggtc gaggcgccgc
     1501 cgccagagcc gacctgggac ggcgtgcgcg accgcctgca gcgcgccgcc gcctcggcct
     1561 gacgcgctgc gtgccacgcg ccggccggcg tcgttaaact acgtggtcct gctttggaga
     1621 agtttttcat catgaacgca tcctggattg gccgcgctgc gcgcaccctg atcggcgcca
     1681 gcgccgtggc cctgctggcc gcctgcggca gcggctcggt cgtgtcggac ctgacgccca
     1741 aacgcttcat caccgtcggc gatggcttca tggacgtcgg ccaggccggc taccgctaca
     1801 ccgtcaacga cggcagcaac atctgggtgc aagacctggc ggcgcattac cagcagacca
     1861 tcacatcggc caacagcggc ggctggggct atgcccaggg cggggcgcgc gtggcggcgg
     1921 ccgacgcggg cggcgccccc agcgtgacgg cgcagatcga cgccgtgctg gcgcgcacca
     1981 ccttcaacgc caacgacgac gtggtggtcg tgggcggcgg catcgccgac gtggtggctg
     2041 ccgtcggcgc cactggcttt accgaccaga ccaagaaaga tgtacaggcc gccggcaagg
     2101 cccttggcga gcagatcaag cgcctggtgg acgccggcgc cgcgcatgtg ttggtggtgg
     2161 gcgtgtacaa cctgggcaac acgccgtggg cggcagggca gaatcagaaa agcgcactga
     2221 ccgacctgtc ggtggatttc aacaccggcg tgcagctcga aatcgtcaac ctgggcaaga
     2281 acgtgctgtt cgtcgacccc gccctgctgc acaacctgat gtacaacaag cccgagaact
     2341 accagttcga caacggccgc gacgcggtgt gcaccacgcc cgatgccacc acctgcaaca
     2401 cgtcgacgct ggtggcaggg gccgaccaca acaagtggct gtatgccgat ggcctgtacc
     2461 tgacgcccga ggcctcgcgc cgcttcggct ccgatcggta ttccgaaagc gtgtactaca
     2521 aattcaagaa ccgctggtga tcgtctacgg ctccaaccga aaaagcggcc cgcgggcc
//
LOCUS       DAOMVO010000340         2578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11877_length_2578_cov_13.073722, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000340 DAOMVO010000000
VERSION     DAOMVO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11877_length_2578_cov_13.073722"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="PKV30_12485"
     CDS             <1..162
                     /locus_tag="PKV30_12485"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541734.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRL31066.1"
                     /translation="FLHGRIRFDRIHAVNDATLGAVALSKPASLGDLLALDASARREA
                     ESLVARWAT"
     gene            251..1624
                     /gene="rseP"
                     /locus_tag="PKV30_12490"
     CDS             251..1624
                     /gene="rseP"
                     /locus_tag="PKV30_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRL31067.1"
                     /translation="MLLTLVAFVVALGLLIAVHEWGHYRMAVARGVKVLRFSVGFGKT
                     IARFKPKKQRPGQDTEFVIGAIPFGGYVRMLDEREAPVADGERHLAFNTQPLKSRALI
                     VAAGPVANLLLAVLLYALVNWIGVNEPRALLSPPVAGSLAQKAGLQGGETVRQAGLAG
                     DELKPVASFETLRWVLTRGALDGQDVVLDVARDGGRGREVTLPLSSLATRDPDANLFR
                     AIGIVSPLSQPVMGEIMAGGAAQRAGLQQGDVVRRVGSTEIVDGQQLRELIRASGQSG
                     SATATEWQIERDGRAMQLQVQPDVQDDKGQPVGRIGAYVGSLPEMVKVRQGPVDGLWS
                     GVVRVWEVSTLSLKLLGRMLTGELSLKNLSGPIAIADYAGKSASMGLTYYLGFLAFIS
                     VSLGVLNLLPVPVLDGGHLMYYLWEAVTGKPVTGVWLERLQYVGMSLLLAMMAIAMYN
                     DIVTRLG"
     gene            1734..>2578
                     /gene="bamA"
                     /locus_tag="PKV30_12495"
     CDS             1734..>2578
                     /gene="bamA"
                     /locus_tag="PKV30_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRL31068.1"
                     /translation="MSLAAQLAWAVDPFTVRDIRVEGLQRVEPGTVFASLPVRVGETY
                     NDDKGAAAIRALFGLGLFNDVRLEVQGDVLVVIVQERPTVAAVEFAGNKEFTNEVLQG
                     VLRDAGLAAGRPYDRALADRAEQELKRQYINRSLYGAEVVTTVTPSERNQVRLTFNIT
                     EGRPARIKDIDIIGNQAFSDSRLKDLFDLDSGGWLSWYTKSDQYSRAKLNADLEALRS
                     FYLARGYLDFKIDSTQVAMAPDKESITITVNITEGDRFAVSGVTLDGNYLDKDDEFKS
                     LIKIKP"
BASE COUNT          406 a          800 c          896 g          476 t
ORIGIN      
        1 tttttgcacg ggcgcatccg cttcgaccgc atccacgccg tcaacgatgc aactttgggc
       61 gcggtggcac tgtccaagcc ggcgtcgctc ggcgatctgt tggcgctgga cgccagcgcg
      121 cggcgtgagg ccgaatcgct ggtggcgcgc tgggccacct gatcgggccg agttttgagt
      181 aaaaagtggc tgcggcgctt gtctatcaag cgcaagcagc tattatttta atagagaacg
      241 caggtactgc atgttgctga cgctggttgc ttttgtcgtc gccctggggc tgctgattgc
      301 cgtgcacgaa tggggccact accgcatggc ggtggcgcgc ggcgtcaagg tgctgcgctt
      361 ttccgtcggt ttcggcaaga cgattgcgcg cttcaagccc aagaagcagc gcccgggcca
      421 ggacaccgaa tttgtcatcg gcgccattcc cttcggcggc tacgtcagga tgctggacga
      481 gcgcgaggcg ccggtggccg atggtgagcg gcacctggcc ttcaacacgc agccgctgaa
      541 gtcgcgcgcc ctcatcgtgg ccgccgggcc ggtggccaac ctgttgctgg cggtgctgtt
      601 gtacgcactg gtcaactgga tcggcgtgaa cgagccgcgc gcgctgctgt cgccgccggt
      661 cgctggctcg ctggcgcaga aggccggcct gcagggcggc gaaacggtgc gtcaggcggg
      721 cttggcgggc gatgagctga agccggtggc ctcttttgaa accctgcgct gggtgctgac
      781 acgtggcgcg cttgatgggc aggacgtggt gctcgacgtg gcgcgcgatg gcgggcgcgg
      841 gcgtgaggtg acgctgccgc tgtccagctt ggcgacgcgc gaccctgatg cgaatctgtt
      901 tcgcgccatc ggcatcgtct cgccgctcag ccagccggtg atgggcgaga tcatggcggg
      961 tggtgctgcc cagcgcgccg ggttgcagca gggcgatgtg gtgcggcgcg tgggcagcac
     1021 cgagatcgtt gacggccagc aactgcgcga gctgatccgc gccagcgggc agagcggcag
     1081 cgccaccgcc accgaatggc agatcgagcg cgatggccgc gcgatgcagc tgcaggtgca
     1141 acccgacgtg caggacgaca agggccagcc ggtcgggcgc atcggcgcct acgtgggcag
     1201 cctgcccgag atggtcaagg tgcgccaggg gccggtcgac ggcctgtggt cgggcgtggt
     1261 gcgggtgtgg gaagtgtcga ccttgtcgct caagctgctg ggtcgcatgc tgacgggcga
     1321 gctgtcgctg aagaacctct ctggccccat cgccatcgcc gattacgcgg gcaagtcggc
     1381 ctcgatgggg ctgacttatt acctgggctt tctggccttc atcagcgtca gcctcggcgt
     1441 gctgaacctg ctgccggtgc cggttctgga tggggggcac ctgatgtatt atctttggga
     1501 ggccgtgacg ggcaagcccg tgacgggcgt ttggctggag cgccttcagt acgtgggcat
     1561 gagtttgttg ctggccatga tggccatcgc gatgtacaac gacatcgtca ctcgcctggg
     1621 ctgaccgatt cgcttcttcc gctggcctcg cgcggccgca gtttcctctc aagcattcca
     1681 tgaaatccat cgcacaacgt ttccgtctgc ggtctgtcgc ctcgctggtg gcgatgtctt
     1741 tggcggcgca actggcctgg gcggtcgacc cgttcaccgt gcgagacatt cgcgttgaag
     1801 gcctgcagcg cgtggagccg ggcaccgtgt tcgcctcgct gccggtgcgc gtgggggaga
     1861 cctacaacga cgacaagggc gccgccgcca tccgcgcgct gttcgggctc ggcctgttca
     1921 acgacgtgcg gcttgaagtg cagggcgacg tgctggtggt gatcgtgcag gagcggccca
     1981 cggtggccgc ggtcgaattc gccggcaaca aggaattcac caacgaggtg ctgcaaggtg
     2041 tgctgcgcga tgccggcctg gccgccggcc gcccctacga ccgcgcgctg gccgaccgcg
     2101 ccgagcagga gctcaagcgc cagtacatca accgcagcct gtacggcgcc gaggtggtga
     2161 ccaccgtcac gccgtccgag cgcaaccagg tgcgcctgac cttcaacatc accgaaggcc
     2221 ggcctgcgcg catcaaggac atcgatatca tcggcaacca ggcgttctcg gattcgcgcc
     2281 tgaaagacct gttcgacctc gactccggtg gctggctgtc gtggtacacc aagagcgacc
     2341 agtattcgcg cgccaagctc aacgccgacc tggaggcgct gcgctcgttc tacctggcgc
     2401 gcggctatct cgacttcaag atcgattcca cccaggtcgc catggcaccg gacaaggagt
     2461 cgatcaccat caccgtcaac atcactgaag gtgatcgctt cgcggtgtcg ggcgtcacgc
     2521 tggacggcaa ttacctggac aaggacgacg agttcaagtc gctcatcaag atcaagcc
//
LOCUS       DAOMVO010000341         2575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11894_length_2575_cov_12.661111, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000341 DAOMVO010000000
VERSION     DAOMVO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11894_length_2575_cov_12.661111"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..628
                     /gene="aqpZ"
                     /locus_tag="PKV30_12500"
                     /pseudo
     CDS             1..628
                     /gene="aqpZ"
                     /locus_tag="PKV30_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            complement(760..1305)
                     /locus_tag="PKV30_12505"
     CDS             complement(760..1305)
                     /locus_tag="PKV30_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL31069.1"
                     /translation="MRIPDWTPDTKPQPDELVAAIRARRGGDLINLDRALLWSEPLAR
                     GWNVYLKAVRTELPTSRKLRELAICTVALLTGAEYEYHHHAPDFLTAGGTQVELAALG
                     RVVAADPRRSAADPALGKVEQLVIRYAAQMTRDVTVDDALFGALRQHFDTTQIVELTS
                     AIATYNMVARFLVALGVSPET"
     gene            1428..>2575
                     /locus_tag="PKV30_12510"
     CDS             1428..>2575
                     /locus_tag="PKV30_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRL31070.1"
                     /translation="MKRRTTWIALLAALVVAVAAFWLWRGRAVPVDVVEVRSAPLVRT
                     LQFSARVATTSRVNLGSTQTGRVTAVALREGARVQRGDVLVQLETDELRSALAQAVAA
                     ERQAAARVQSLRGSGRSVAQAQVAQAQATLHAAERELARTQQLVAQGFVSAARLDDAR
                     KAVALARAQQDSARAQQQTVGDGGPDLALAEAQRTQAQAAVQAAEARLAQATVRAPAD
                     GRVLTRSVEPGQIVQPGAALMSLALEGPTQLVAQVDERFLDQLQAGQPARVVADAFAG
                     QPFAARVLTIAPAVDAQRGSVEVKLAPDGAAPAFLREDMTLSVEVQTGRRDAALVLPT
                     RALRGAGAPGEGTVWVVQGGQVQARAIGLGLRTLDAVEAARGLAAGDAV"
BASE COUNT          338 a          838 c          995 g          404 t
ORIGIN      
        1 ggcagcgcgg tgctggcggc ggcgtttccg cagctgggca tcggctttca cggcgtggcg
       61 ctggcgtttg gcctgacggt gctgacgatg gcctacgcca tcggccacat ctcgcgctgc
      121 cacctgaacc cggcggtgag ctttggcctg tgggccggcg gctgctttcc gaccaaggag
      181 ctggtgcctt accggcggct caggtgattg gcgccatcgc ggcggcggcc gtgctgtacc
      241 tcatcgccag cggcaagccg ggttttgagg tgggcggctt tgccaccaac ggctatggcg
      301 atctgtcgcc ggcaagtact cgatggtgtc ggtgctggtg tgcgaggtgg tgatgacggc
      361 gatgttcctg ttcgtcatca tgggctcgac ccacggcaag gcgccggccg gctttgcgcc
      421 gatcgccatt ggcctgtgcc tgacgctgat tcacctggtc agcattccgg tgtcggacac
      481 ttcggtgaac ccggcgcgct cgaccggcgt ggcgctgttt gcgcagaccg gcgcgctaag
      541 tcagttgtgg ctgttctggg tggcgccgat tgtgggcggc atgatcggcg cggtggtcta
      601 caaggcggtg gcgggcgctc gcgactgaca gcggcctccc ggctaatcgg ctgagaaatc
      661 gccgccaccg gttgccggtg gcggcgattt gctattatta atatagctgc tcgcgcttgt
      721 ccagcaagcg ccaggggcta aaaaggctta aaagtcaggt cacgtctccg ggctcacccc
      781 cagcgccacc agaaaacgcg ccaccatgtt gtaggtggcg atggcgctgg tcagctccac
      841 gatctgcgtg gtgtcgaagt gctggcgcag cgcgccgaac agcgcgtcgt cgacggtcac
      901 atcgcgcgtc atctgcgcgg catagcgaat caccagttgc tcgaccttgc cgagcgccgg
      961 gtcggcggcg ctgcggcgcg ggtcggccgc cacgacgcgg ccgagcgcag ccagctcgac
     1021 ttgcgtgccg ccggcggtaa gaaaatccgg cgcgtggtgg tggtattcgt actcggcgcc
     1081 ggtcagcagc gccacggtgc agatggccag ctcgcgaagc ttgcgactgg tgggcagctc
     1141 ggtgcgcacg gctttcaggt acacgttcca gccgcgcgcc agcggctcgc tccacagcag
     1201 ggcgcggtcg aggttgatga ggtcgccgcc gcgccgcgcg cggatggcgg ccaccagttc
     1261 gtcgggctgc ggcttggtgt cgggggtcca atcggggatg cgcatgcggg gctccttgca
     1321 cgggggtagc gccaagcata ggccgggccg cgcccgataa tggaacacat gcgcaaccca
     1381 ccacccccaa ccgtgacgca gcgccgcgtt tttgcacggg gcgcgcgatg aagcggcgca
     1441 ccacctggat cgcgctgctg gcggctttgg tcgtggcggt ggcggcgttt tggctgtggc
     1501 gcgggcgtgc cgtgccggtc gatgtggtcg aggtgcgcag cgcgccgctg gtgcgcacgc
     1561 tgcagttctc ggcccgcgtg gcgaccacct cgcgcgtcaa cctgggcagc acccagactg
     1621 gccgcgtgac ggccgtcgcc ctgcgcgaag gtgcgcgcgt gcagcgcggc gacgtgctgg
     1681 tgcaattgga aaccgatgag ctgcgcagtg cgctggcgca ggccgtggcg gctgagcgcc
     1741 aggccgccgc gcgcgtgcaa agcctgcggg gcagcgggcg ctccgtcgcg caggcgcaag
     1801 tggcgcaggc acaggccacg ctgcatgcgg ccgagcgcga actggcgcgc acgcagcaac
     1861 tggtggcgca gggctttgtc agcgccgcgc ggcttgacga cgcacgcaag gccgtggccc
     1921 tggcgcgcgc ccagcaggac agcgcgcgcg cccagcagca gacggtgggc gatggcgggc
     1981 ccgacctggc gctggccgag gcgcagcgca cccaggcgca ggcggcggtg caggcggccg
     2041 aggcgcggct ggcgcaggcc accgtgcgcg cgccggccga tggccgcgtg ctcacgcgca
     2101 gcgtggagcc agggcagatc gtgcagcccg gcgccgcgct gatgagcctg gcgctggagg
     2161 gcccgacgca gctggtggcg caggtggacg agcgcttttt ggaccagctg caagccggcc
     2221 agcccgcgcg cgtggtggcc gatgcctttg ccggtcagcc gtttgccgcg cgcgtactca
     2281 ccattgcgcc agcggtggat gcgcagcgcg gctcggtgga agtcaagctg gcgcccgacg
     2341 gtgccgcgcc ggcctttctg cgcgaggaca tgacgctctc ggtcgaagta cagaccggcc
     2401 ggcgcgacgc agccctggtg ctgcccacgc gcgcgctgcg cggcgccggc gcgccgggcg
     2461 aaggcacggt gtgggtggtg cagggcgggc aggtgcaggc gcgcgccatc ggcctcggcc
     2521 tgcgcacgct ggatgcggtg gaggctgcgc gcggcctggc ggcgggcgat gcggt
//
LOCUS       DAOMVO010000342         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11907_length_2573_cov_12.613582, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000342 DAOMVO010000000
VERSION     DAOMVO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11907_length_2573_cov_12.613582"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /gene="dapA"
                     /locus_tag="PKV30_12515"
     CDS             <1..809
                     /gene="dapA"
                     /locus_tag="PKV30_12515"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRL31071.1"
                     /translation="RKLIDWHIAEGTDCIGVVGTTGESPTVNVEEHQEIIRVSVEQAA
                     KRVPIMAGCGANSTAEAIELARFAKQVGADCQLQVVPYYNKPTQEGQYQHFKAIAEAV
                     GDLPMYLYNVPGRSVADMLHDTVLRLAQVPGIVGIKEATGNIERAQWLIRDVPKGFAV
                     FSGDDPTAVALMLCGGQGNISVTANVAPRLMHDLCVAAIAGDREKAMQIQFQLMPLHK
                     QLFVEANPIPVKWAMARMGLMGGAMRLPMTPLSAANEPVVERALNDAGLL"
     gene            891..2003
                     /gene="bamC"
                     /locus_tag="PKV30_12520"
     CDS             891..2003
                     /gene="bamC"
                     /locus_tag="PKV30_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HRL31072.1"
                     /translation="MPTQRSCTTLLGAALLLAAGGCSVLEPDKIDYKSSGRGVSLEVP
                     PDLTQLPGQSRYAAQSGAVTASGYQAGQVATAAAGTPTAANKVADVQFMRQDGERWLR
                     VDRAPDQLWGPVRDFWLESGFLMAIDQANMGLMETDWAENRAKIPQDFIRNTIGKVFD
                     SLYSTGERDKFRTRLERNANGGTDIFVTHRGMEEVYSSARRDSTVWQPRARDPELEAE
                     FMRRMMVKLGVSQEQAKAALATAATTQAAAPNARVVAAAGGAPAVQLAEDFDRAWRRV
                     GLALDRTGFTVEDRDRSKGLYFVRYIDPTVEKKEAGFFGRLLGRGSQQLPTSQYQIAV
                     RSEGQSSVVTVNDRAGAPAASADAQRIVKVLADELK"
     gene            2014..>2573
                     /locus_tag="PKV30_12525"
     CDS             2014..>2573
                     /locus_tag="PKV30_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL31073.1"
                     /translation="MLRFRSLASGSSGNATLIEGSDGLHRTRVLVDCGLGLRQLIARL
                     AVEGIGPADLDGIFITHEHGDHIGCAPMLVARYGVPLWTSAGTAQYAAFAGLESALNL
                     VRDGQVFAIGGLQLHPFTVPHDAREPLQLRCTDGDRVLGLMTDIGHVTGHALAALAGC
                     HALVLESNHDVELLAQSRYPDFLKRRV"
BASE COUNT          442 a          878 c          864 g          389 t
ORIGIN      
        1 tgcgcaagct gatcgactgg cacatcgccg aaggcaccga ctgcatcggc gtggtcggca
       61 ccacgggcga atcgcccacg gtgaacgtcg aagagcatca ggagatcatt cgcgtctcgg
      121 tcgagcaggc ggccaagcgc gtgcccatca tggccggctg cggtgcaaac tcgacagccg
      181 aagccatcga gctggcgcgg ttcgccaagc aggtcggtgc cgactgccag ttgcaggtgg
      241 tgccctacta caacaagccg acgcaagaag gccagtacca gcacttcaag gccattgccg
      301 aggcggtggg cgacctgccc atgtacctgt acaacgtgcc cggccgcagc gtggccgata
      361 tgttgcacga cacggtgctg cgcctggccc aggtgccggg catcgttggc atcaaggaag
      421 ccaccggcaa catagagcgc gcgcagtggc tgatccgcga cgtgcccaag ggttttgccg
      481 tcttctcggg cgacgacccc acggccgtgg cgctgatgct gtgcggcggc cagggcaaca
      541 tcagcgtcac cgccaacgtg gcgccgcgcc tgatgcacga tttgtgcgtg gccgccatcg
      601 cgggcgaccg cgagaaggcc atgcagatcc agttccagct gatgccgctg cacaagcagt
      661 tgtttgtcga ggccaacccg atccccgtca agtgggccat ggcgcgcatg ggcctgatgg
      721 gcggcgccat gcggctgccg atgacgccgc tgtcggcggc caacgagccg gtggtcgagc
      781 gcgccttgaa cgacgccggc ctgctctgaa atcactacat aaataatagc tgcttgcgct
      841 tgccagacaa gcgccggcgc ccgatttgat tcaaaggaat atccaacgtg atgcctaccc
      901 aacgctcttg caccaccttg ctcggcgccg ccttgctgct ggcggccggc ggctgctcgg
      961 tgcttgaacc cgacaagatc gactacaaga gttcgggccg cggcgtctcg ctcgaggtgc
     1021 cgcccgatct gacccagttg cccggccagt cgcgctatgc cgcgcaaagc ggcgccgtca
     1081 cggccagcgg ctaccaggcc ggccaggtgg cgacggctgc cgccggcacg ccgacggcag
     1141 ccaacaaggt ggccgacgtg cagttcatgc gccaggacgg cgagcgctgg ctgcgcgtgg
     1201 accgcgcgcc cgaccagttg tggggcccgg tgcgcgactt ctggcttgaa agcggcttct
     1261 tgatggccat cgatcaggcc aacatgggcc tgatggaaac cgactgggcc gagaaccgcg
     1321 ccaagatccc gcaggatttc atccgcaaca ccatcggcaa ggtgttcgat tcgctctatt
     1381 ccaccggcga gcgcgacaag ttccgcaccc ggctggagcg caacgccaac ggcggcaccg
     1441 acatcttcgt cacgcaccgc ggcatggaag aggtgtattc gtcagcccgc cgcgacagca
     1501 ccgtctggca accgcgcgcg cgcgatcccg agctggaggc cgaattcatg cgccgcatga
     1561 tggtcaagct cggcgtctcg caagagcagg ccaaggccgc gctggccacc gccgccacga
     1621 cgcaggccgc ggcgcccaac gcccgcgtgg tggccgcggc cggcggtgcg ccggccgtgc
     1681 aactggcgga agactttgac cgcgcctggc gccgcgtggg gctggcgctc gatcgcaccg
     1741 gcttcaccgt ggaagaccgc gaccgcagca agggcctgta cttcgtgcgc tacatcgacc
     1801 ccacggtgga gaagaaagaa gccggcttct tcggccgcct gctcggccgc ggcagccagc
     1861 agttgcccac cagccagtac cagatcgcgg tgcgcagcga aggccagagc agcgtcgtga
     1921 cggtcaacga ccgcgctggc gcgccagcgg catcggccga tgcgcagcgc atcgtcaagg
     1981 tgctggccga cgagctgaag taagcgcggc tcgatgctgc gttttcgcag cctggccagc
     2041 ggcagctcgg gcaacgccac gctcatcgag ggcagcgatg gcctgcaccg cacccgcgtg
     2101 ctggtcgatt gcgggctggg cctgcgccaa ctgattgcgc ggctggcggt tgagggcatc
     2161 ggccccgccg atctggacgg catcttcatc acgcacgagc acggcgacca catcggctgc
     2221 gcgcccatgc tggtggcacg ctacggcgtg ccgctgtgga ccagcgccgg caccgcgcag
     2281 tacgcggcgt ttgccgggct tgaatcagcg ctgaacctgg tgcgcgacgg ccaggtgttt
     2341 gccatcggcg gcctacagct tcaccccttc accgtgccgc acgacgcgcg cgaaccgctg
     2401 caactgcgct gcaccgatgg cgaccgcgtg ctgggcctga tgaccgacat cggccacgtc
     2461 acgggccatg cgctggccgc gctggcgggt tgccacgcgc tggtgctgga gagcaaccac
     2521 gacgtcgagt tgctggcgca atcgcggtat ccggactttt tgaagcgccg cgt
//
LOCUS       DAOMVO010000343         2567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11951_length_2567_cov_13.611863, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000343 DAOMVO010000000
VERSION     DAOMVO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11951_length_2567_cov_13.611863"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKV30_12530"
     CDS             <1..554
                     /locus_tag="PKV30_12530"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRL31074.1"
                     /translation="HLSHFVSQYGYWAVAAGCLLEGETVMVLAGLAARRGLLDLPLVM
                     ALGSAMGALGDITMFSLGRWRGAAVLARWPRLLGYQERLNRLLARWGAGVVVGVRFMY
                     GMRIAGPILLGMSDLPWPRFLAFNLLGAALWGFGVAGAGWLFGHAAEALLGEVHDAEK
                     WIVLALVVVLGGVAVWRWWRHRV"
     gene            complement(627..2024)
                     /gene="gorA"
                     /locus_tag="PKV30_12535"
     CDS             complement(627..2024)
                     /gene="gorA"
                     /locus_tag="PKV30_12535"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HRL31075.1"
                     /translation="MTNQQTFDFDLYVIGGGSGGVRAARMAASRGVRVGMAESGRMGG
                     TCVNVGCIPKKLYSYAAHYAEAFVEARGFGWELAHGPTLDWPTLKANRAKEITRLNGI
                     YDGLMLNAGVQRLQGHARLVDAHTVEVTAADGSSTRHTARNIVIATGGTPSVPEVEGH
                     ELVVTSDDMFDLPDFPRRLVVVGGGYIGCEMASIFHGLGAQTTLLYRGEQILRGFDDE
                     VRDFTADEMRKAGVDVRVHCDVARIAPGAECARQVVLSDGTVIEADVVLYATGRRPNV
                     EGLGLAEVGVKQASDGTIEVDANFATSVPGVYALGDVVGRMELTPVALAEGMALVDHL
                     FGPAPGKALRTMDYDNIPTAIFTHPPVGTVGLSEAHAREKFSAIQVFRSDFKPLRHTL
                     SGRGERTLVKLIVDSATDRVVGLHMVGADAGEIVQGFAVALKCGATKAQFDATIGIHP
                     TSAEEFVTLREVART"
     gene            complement(2190..>2567)
                     /locus_tag="PKV30_12540"
     CDS             complement(2190..>2567)
                     /locus_tag="PKV30_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRL31076.1"
                     /translation="FAMDSGAARASQASAGGLKDIKTTAQDNAPRISAPQDGTILALD
                     PDIPPARQRLTLLADAGGAPAAQLRWWIDDRPIGSGARAQWLPWPGRHVVQLRDAQGR
                     VHDERRIQVRGAVAKTASPRKPP"
BASE COUNT          333 a          840 c          926 g          468 t
ORIGIN      
        1 atcacctgtc gcatttcgtc tcgcaatacg gctactgggc cgtggccgcc ggctgcctgc
       61 tggagggcga gaccgtgatg gtgctggccg ggctggccgc gcgacgcggc ttgctcgatc
      121 tgccattggt gatggcgctg ggctccgcca tgggtgcgct cggcgacatc accatgttca
      181 gcctggggcg ctggcgcggc gcggcggtgc tggcgcgctg gccgcgcctg ctgggctatc
      241 aggaacggtt gaaccgcctg ctggcgcgct ggggcgccgg cgtggtggtg ggcgtgcgct
      301 tcatgtacgg catgcgcatc gcggggccga ttctgctggg catgtcggac ttgccgtggc
      361 cgcgctttct ggccttcaac ctgctgggcg cggcgttgtg gggctttggg gtggccggtg
      421 ccggctggct gttcggccat gcggccgagg cgctgctggg tgaggtgcac gatgccgaga
      481 aatggatcgt gctggcgctg gtggtggtgc tgggtggcgt ggctgtgtgg cgctggtggc
      541 gccatcgcgt ctgacgagaa ttcaagtcat ttaggccgct ggcgcttgtc tggcaagcgc
      601 gagcagctat caatacagaa gcgctttcag gttctggcca cctcgcgcag cgtgacgaac
      661 tcttccgcgc tggtcgggtg gatgccgatg gtggcgtcga actgggcctt ggtggcgccg
      721 catttcagcg ccacggcaaa gccctgcacg atctcgccgg catcggcgcc caccatgtgc
      781 aggccgacca cgcggtcggt ggcgctgtcc acgatcagct tgaccagcgt gcgctcgccc
      841 cggcccgaca gcgtgtgcct gagcggcttg aaatcgctgc gaaacacctg gatggcgctg
      901 aatttctcgc gcgcatgcgc ttcgctcagg cccacggtgc cgaccggcgg gtgggtgaag
      961 atggcggtcg ggatgttgtc gtaatccatg gtgcgcagcg cctttccggg cgccgggccg
     1021 aacaggtgat ccaccagcgc catgccttcg gccagcgcga ccggcgtcag ctccatgcgg
     1081 ccgaccacat cgcccagcgc gtagacgccg ggcacgctgg tggcgaaatt cgcatcgact
     1141 tcgatggtgc cgtcgctggc ctgcttcacg ccgacttccg ccaggcccag cccctcgacg
     1201 ttcggccggc ggccggtggc gtacagcacc acgtcggctt cgatcacggt gccgtcgctc
     1261 agcaccacct ggcgcgcgca ttcggcgccg ggggcgatgc gcgccacgtc gcagtgcacg
     1321 cgcacgtcga cgccggcctt gcgcatctcg tcggcggtga agtcgcgcac ttcgtcgtcg
     1381 aagccgcgca ggatctgctc gccgcggtac agcagggtcg tttgcgcgcc caggccatgg
     1441 aagatgctgg ccatctcgca gccgatgtag ccgccgccca ccaccaccag gcggcgcggg
     1501 aagtcgggca ggtcgaacat gtcgtcggaa gtgacgacga gctcgtgccc ttccacctcg
     1561 ggcaccgagg gcgtgccgcc ggtggcaatc acgatgtttc tggccgtgtg ccgcgtgctg
     1621 ctgccgtcgg cggcggtgac ttccaccgtg tgcgcatcga ccaggcgcgc gtggccttgc
     1681 agccgttgca cgccggcgtt gagcatcagc ccgtcgtaga tgccgttcag gcgcgtgatc
     1741 tctttcgcgc ggttggcctt gagcgtgggc cagtcgagcg tcgggccgtg cgccagttcc
     1801 cagccgaagc cgcgcgcctc gacaaacgct tcggcgtagt gcgccgcgta gctgtagagc
     1861 ttcttcggga tgcagccgac gttgacgcag gtgccgccca tgcgcccgct ctcggccatg
     1921 cccacacgca cgccgcgcga tgccgccatg cgcgccgcgc gcacgccgcc ggagccgccg
     1981 ccgatgacgt acaaatcgaa atcaaaagtc tgctggttgg tcatcggccg attgtccggc
     2041 agggcgcaat ggcgaggcgc gcggcgggtt aaacatgacc tcgcttcgct caatgaccgg
     2101 cgctggcgcg ccatgacaaa tgacaaagtg catcttgcgg cgcagcggtt ttgtcatttg
     2161 tcatgcgctc gtcaaagcgc ggtcatttgt catggtggtt tgcgcggcga cgccgtcttc
     2221 gccaccgcgc cacgcacctg gatgcggcgc tcgtcgtgca cgcggccttg cgcgtcgcgc
     2281 aactgcacca cgtggcggcc gggccagggc agccattggg cgcgcgcgcc gctgccgatg
     2341 gggcggtcgt caatccacca gcgcagttgc gcggcgggcg cgccgccggc gtcggccagc
     2401 agggtcaggc gctggcgcgc gggcgggatg tcggggtcga gcgcgaggat ggtgccgtct
     2461 tgcggcgcgc tgatgcgcgg ggcgttgtcc tgcgccgtgg ttttgatgtc ttttaggccg
     2521 ccagcgcttg cctggcttgc gcgagcagct ccgctatcca tagcaaa
//
LOCUS       DAOMVO010000344         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_11988_length_2563_cov_11.744019, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000344 DAOMVO010000000
VERSION     DAOMVO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11988_length_2563_cov_11.744019"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1186)
                     /gene="moaA"
                     /locus_tag="PKV30_12545"
     CDS             complement(29..1186)
                     /gene="moaA"
                     /locus_tag="PKV30_12545"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRL31077.1"
                     /translation="MGERVIPLVDHRTVGLAVPASAAPAPAGTLVDTRGRPLRDLRIS
                     VTDRCNFRCSYCMPKDVFNNEYAYLPHSALLSFEEITRLATRFVAHGVHKLRLTGGEP
                     LLRKGLETLIAQLAALRTPDGQPLDITLTTNGSLLARKAQALKSAGLNRVTVSLDGLD
                     DATFRAMNDVDFPVADVLAGIEAARAAGLGQYPDGRPGALKVNMVVKRGTNDDQILPM
                     ARHFRATGVTLRFIEYMDVGATNGWRMDEVLPSAEVVKRLQAEFALVPLQASAPGETA
                     ERWGYAGADGRHDPLLGEVGFISSVTQAFCGDCNRARLSMDGKLFLCLFAGQGYDLRA
                     LMRGGANDAQLDAAMAAIWQGRSDRYSELRASLPADVQQAVRRVEMSYIGG"
     gene            1291..1812
                     /locus_tag="PKV30_12550"
     CDS             1291..1812
                     /locus_tag="PKV30_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRL31078.1"
                     /translation="MHRLALSRRQLLQAGAAASVLTAPWAAAQKAGATPLPAVGAPYP
                     LAAVPLLGGGRFVPAQAEGRVLVLYWWASWCPFCAQQSPEMQKLWAAQQDRGLRMLAL
                     SIDKKPEDAANYLRQKGYTFPVVWTSPEVARAYPKPEGLPVTVVRGKDGRVVQAEKGQ
                     LFAEDVVDLARWL"
     gene            complement(1891..2451)
                     /locus_tag="PKV30_12555"
     CDS             complement(1891..2451)
                     /locus_tag="PKV30_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HRL31079.1"
                     /translation="MTAPLAGTQQGLTVTKGFSPSTVAPGGSSQLTVTLTAPAATCAP
                     APTTPIYTGLGFTDTLPAGMTLAAAPAASTTCAGATLAATGGADSFSLSGASLAPGAS
                     CTATVTVAVAGAGTFHNVISASMGQPGAVTNDQAVPALADAIANLQAGIAPVAPVPTL
                     GEAALMAMGLLLGAMGLRRLRPARRS"
BASE COUNT          367 a          859 c          930 g          407 t
ORIGIN      
        1 gcgatatctg catgggtagg cattgcatct accccccgat gtaactcatc tccactcttc
       61 gcaccgcctg ctgaacatcg gccggcaggc tggcgcgcag ctcgctgtag cggtcgctgc
      121 ggccttgcca gatggcggcc atcgccgcgt cgagttgcgc atcgttggcg ccgccgcgca
      181 tcaaggcccg caggtcgtag ccctggccag cgaacaggca caggaacagc ttgccgtcca
      241 tcgacagccg cgcgcggttg cagtcgccgc aaaacgcctg cgtcacgctg ctgatgaagc
      301 cgacttcgcc gagcagcggg tcgtgccgcc catcggcacc ggcataaccc cagcgctcgg
      361 cggtttcgcc cggcgcgctg gcttgcagcg gcaccagcgc gaattcggct tgcaggcgct
      421 tgacgacttc ggccgatggc aacacctcgt ccatgcgcca gccgttggtg gcgcccacgt
      481 ccatgtattc gatgaagcgc agcgtgacgc cggtggcgcg aaagtgccgc gccatgggca
      541 ggatctggtc gtcgttggtg ccgcgcttga cgaccatgtt gaccttcagg gcgcccgggc
      601 gcccgtccgg gtactggccc aagcccgccg cgcgcgccgc ctcgatgccg gccagcacgt
      661 cggcgactgg aaagtccacg tcgttcatgg cgcggaaggt ggcgtcatcc aagccatcca
      721 ggctgacggt gacgcgattc aggccggcgc ttttcagcgc ctgcgccttg cgcgcgagca
      781 gcgacccgtt ggtggtcagc gtgatgtcga gcggctggcc atccggtgtg cgcagtgcgg
      841 ccaattgcgc gatcagcgtc tccagcccct tgcgcagcag cggctcgccg cccgtcaggc
      901 gcagcttgtg cacgccgtgg gccacaaagc gcgtggccag gcgcgtgatt tcctcgaacg
      961 acagcagcgc gctgtgcggc aggtaggcgt attcgttgtt gaacacgtcc ttgggcatgc
     1021 agtagctgca gcggaagttg cagcggtcgg tgacgctgat gcgcaggtcg cgcagcggcc
     1081 gaccgcgcgt gtccaccagc gtaccggccg gcgcgggcgc ggccgacgcc ggcaccgcca
     1141 agccgacggt gcggtgatca accaagggaa tgacgcgttc gcccatgcgg caaattatcc
     1201 caaagcgcct gaatccgcct tgcagcaagg ccatcgggcc gcgctaccat gggctgcatc
     1261 gtccgccgcc agccaccgaa ggatttcgcc atgcaccgcc ttgccttgtc acgccgtcag
     1321 ctgttgcaag cgggcgccgc tgcgagtgtg ttgaccgcgc cctgggccgc cgcgcagaag
     1381 gccggcgcga cgccgttgcc ggcggtgggc gcgccctatc cgctggccgc cgtgccgctg
     1441 ctgggcggcg gccgcttcgt gccggcgcaa gccgaaggcc gcgtgctggt gctgtactgg
     1501 tgggccagct ggtgcccgtt ctgtgcccag caaagccccg agatgcagaa gctgtgggcg
     1561 gcgcaacagg accggggcct gcgcatgctg gcgctgtcga tcgacaagaa gcctgaggac
     1621 gcggccaact acctgcgcca gaagggctac accttccccg tggtgtggac cagccccgaa
     1681 gtggcgcgcg cctaccccaa gcccgagggc ctgccggtga ccgtggtgcg cggcaaggac
     1741 gggcgggtgg tgcaggctga aaaaggccag ttgtttgccg aggacgtggt ggacctggcg
     1801 cgctggctct agctgggccg cagtttgctt ttgacttgat agctgccagc gcccgttgga
     1861 cgggcgccgg atggctgttt gacctctgaa tcagctgcgg cgcgcggggc gcaggcggcg
     1921 cagccccatg gcgcccagca gcaggcccat ggccatcagc gcggcttcgc ccagcgtggg
     1981 caccggcgcc acgggcgcga tgcccgcctg caggttggcg atggcgtcgg ccagcgcggg
     2041 cacggcctgg tcgttggtga ccgcgccggg ctggcccatc gacgcgctga tgacgttgtg
     2101 gaaggtgcct gcccccgcca cggccacggt caccgtcgcc gtgcacgagg cccccggcgc
     2161 caggctggcg ccggacagcg agaacgaatc cgcgccgccg gtggccgcaa gcgtggcccc
     2221 ggcacaggtt gtgctggcgg ccggcgcggc ggccagggtc atgccggcgg gcagggtgtc
     2281 ggtgaagccc aggccggtgt agatgggcgt ggtgggcgcc ggggcgcatg tggccgccgg
     2341 ggccgtcagg gtcacggtga gctggctgct gccgccgggc gccacggtgc tggggctgaa
     2401 gcccttggtc acggtcaggc cctgttgcgt gcccgccagg ggcgcggtca cggtcacgaa
     2461 gttgccgcag gcatccgcgg tattgaagta ggtgcggtac tggccgctgc ctagggcgtg
     2521 tcatcgaatt atgaggatgc ttcgttatca ggagcatgaa tcg
//
LOCUS       DAOMVO010000345         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12172_length_2535_cov_21.666935, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000345 DAOMVO010000000
VERSION     DAOMVO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12172_length_2535_cov_21.666935"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PKV30_12560"
     CDS             <1..1110
                     /locus_tag="PKV30_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRL31080.1"
                     /translation="MEIVHEQRDLERYMREAVKVSNDSPVLLDRFLSDAIECDVDAVR
                     DSSGRVFIGGVMEHIEQAGVHSGDSGCSLPPYYLKQATIDELKRQTAAMAGALEVVGL
                     MNVQFAIQEVPQDDGTKKDVIYVLEVNPRASRTVPFVSKATGIQLAKVAARVMAGQTL
                     DAQGIGAEVTPPYFSVKEAVFPFVKFPGVDTILGPEMKSTGEVMGVGKTFGEAYIKAQ
                     MGAGERLPKPLRADGTPAGKVFLTVKNTDKPTAVKIARDLVAMGFALVATKGTAQAIR
                     DAGIACDTVNKVTEGRPHIVDMIKNGEISLVINTVEERRNAIADSRAIRTSSLLARVP
                     TITTIAGAEAAVEGMKFMDKLGVISVQEMHAQLTA"
     gene            complement(1123..1572)
                     /locus_tag="PKV30_12565"
     CDS             complement(1123..1572)
                     /locus_tag="PKV30_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31081.1"
                     /translation="MQKHMTTGLLGLVIGCWGAPAWSQDAPSLQIVWPPEGASLPLGG
                     GKAQTVGVVVQSNFKLMPAGQCGSDTRCGHLHMKIDPDGDKCNQPGKPYNSMNSDFGG
                     DLIKADFGACQSPKGEHVIGVLLADDRHQPVLVGGKPVTALVRVTTR"
     gene            1681..2316
                     /locus_tag="PKV30_12570"
     CDS             1681..2316
                     /locus_tag="PKV30_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL31082.1"
                     /translation="MTLPVAPEPRQSYHHGNLRQALIDAALALLEEGGVEALSVRSAA
                     QRAGVSPGAPFRHFASRTALLTALAEEATSQLRQRVEAAVQDAAHAAPLARFTSLGQA
                     YLGWACEHPAQFRVVSDRRLIDFDGSERLQADNEAIRQLMGRLLNDGVAPGIDVRLAQ
                     LTARALAYGLARMACDGHLDEWTASGPGGGEAMSAALSFFVQLLQGGPSAG"
     gene            2439..>2535
                     /locus_tag="PKV30_12575"
     CDS             2439..>2535
                     /locus_tag="PKV30_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRL31083.1"
                     /translation="MTTIPLTKRGAEKLKEELHRLKTKDRPEVINA"
BASE COUNT          460 a          828 c          826 g          421 t
ORIGIN      
        1 atggaaatcg tgcacgagca gcgcgacctg gagcgctaca tgcgcgaggc ggtgaaggtc
       61 agcaacgact cgcccgtgct gctggatcgt ttcttgagcg atgccatcga atgtgacgtc
      121 gatgccgtgc gcgattcgtc gggccgtgtg ttcatcggcg gcgtgatgga gcacatcgag
      181 caggccggcg tgcactcggg cgactccggc tgctcactgc cgccttacta cctcaagcag
      241 gccaccatcg acgaactcaa gcgccagacc gccgccatgg cgggcgcgct ggaggtggtc
      301 ggcctgatga acgtgcagtt cgcgattcag gaagtgccgc aggacgacgg cacgaagaag
      361 gacgtgatct acgtgctgga agtcaacccg cgcgccagcc gcaccgtgcc cttcgtcagc
      421 aaggcgaccg gcattcaact ggccaaggtc gcggcgcgcg tgatggccgg ccagacgctg
      481 gatgcgcaag gcattggcgc cgaagtcacg ccgccgtatt tcagcgtgaa agaggcggtg
      541 ttccccttcg tcaagttccc gggcgtcgac accatcctcg gccccgagat gaaatccacc
      601 ggcgaggtga tgggcgtcgg caagaccttt ggcgaggcct acatcaaggc ccagatgggc
      661 gccggcgagc gcctgcccaa gccgctcagg gccgatggca caccggccgg caaggtgttt
      721 ctcacggtca agaacaccga caagccgacg gcggtcaaga tcgcacgcga tctggtggcg
      781 atgggttttg cgctcgttgc caccaagggc acggcgcagg ccatccgcga cgccggcatt
      841 gcttgcgaca ccgtgaacaa ggtcaccgaa ggccggccgc acatcgtcga catgatcaag
      901 aacggcgaga tttcgctggt catcaacacg gtggaagagc gccgcaacgc gattgccgac
      961 agccgcgcca tccgcaccag ctcgctcttg gcgcgcgtgc ccaccatcac caccatcgcc
     1021 ggcgccgagg cggcggtgga gggcatgaag ttcatggaca agctgggcgt catctccgtg
     1081 caggaaatgc acgcccaatt gacggcctga gcgggttgcg cgtcagcgcg tggtcacccg
     1141 caccagcgcg gtgaccggct tgccaccgac cagcaccggt tggtgccggt cgtcggccag
     1201 cagcacgccg atgacatgct cgcccttggg cgactggcag gcgccaaagt cggccttgat
     1261 caggtcgccg ccaaagtcgc tgttcatgct gttgtagggc ttgccgggct ggttgcactt
     1321 gtcgccatcg gggtcgatct tcatgtggag gtggccgcag cgcgtgtcgc tgccacactg
     1381 gccggccggc atgagcttga agttgctctg caccacgact ccgacggttt gcgccttgcc
     1441 gccacccagc ggcagactgg cgccttcggg cggccagacg atctggagcg agggcgcgtc
     1501 ttgcgaccag gcgggcgcac cccaacaacc tatgaccaag cccagcaggc cggtagtcat
     1561 gtgcttttgc atggaaaaac tccttcaatg tcggtgaaaa gttgaacatc gtgatgtaat
     1621 cgatgaatac atccgctgca catactaccc taaagtaatc gctgattact tgaaattttc
     1681 atgaccttac ccgttgcccc cgagccccgg cagagctacc accacggcaa tctgcgccag
     1741 gcgctgatcg acgctgcgtt ggcgttgctc gaagaaggcg gcgttgaggc cttgagcgtg
     1801 cgctccgctg cccagcgcgc cggggtatcg cccggcgcgc cgtttcggca cttcgcctcg
     1861 cgcaccgccc tgctcaccgc gctggccgaa gaggcgacct cgcaattgcg ccagcgcgtc
     1921 gaagcggcgg tgcaggacgc ggcacacgcc gcaccgctgg cacgcttcac cagcctgggg
     1981 caggcctatc tgggctgggc ctgcgagcat ccggcccagt tccgcgtggt cagcgaccgt
     2041 cgcctgatcg atttcgacgg ctctgaacgg cttcaggccg acaacgaagc catccgccaa
     2101 ctcatgggcc ggctgctgaa cgacggggtc gcacctggca tcgacgtgcg tctggcccaa
     2161 ctgacggccc gggcgctggc ctatggcctg gcgcgcatgg cgtgcgacgg ccaccttgat
     2221 gaatggactg caagcggtcc cggcggcggc gaggcgatga gcgccgcgct gtcgttcttc
     2281 gtgcagctgc tccaaggcgg cccgtcggca ggctgagcgg gctgcagccc gcgtctggcg
     2341 gcgccagggg cggcgctctt tggcggcata attgccccta tcaataccgc cgaggggaaa
     2401 cgctcggcgg tttcgctttt gtgcaaggat ttcgagacat gacgaccatt cccctgacca
     2461 aacgcggcgc cgaaaagctc aaggaagagc tgcaccgcct gaagaccaag gaccgccccg
     2521 aggtcatcaa cgcca
//
LOCUS       DAOMVO010000346         2532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12191_length_2532_cov_14.978603, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000346 DAOMVO010000000
VERSION     DAOMVO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12191_length_2532_cov_14.978603"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..720
                     /locus_tag="PKV30_12580"
     CDS             124..720
                     /locus_tag="PKV30_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31084.1"
                     /translation="MLSDVTPFSSGFGGASQSPAIAQAFAHAALDPAGACKQPAAGVV
                     DVAVSLTGPASLTPGTPANYLLRVAHPGVVASTAITVTLTLPTGVTATGTPPVGCTFS
                     SANTIVTCQLPDLSVAGSSNLSIQLVAAAGGAGGNAQSSVPAQTGEVNTANNSAALAI
                     AIGAAPPSPTAVPTLDVGALFALGGLLPLVAARGRRQN"
     gene            complement(769..2484)
                     /locus_tag="PKV30_12585"
     CDS             complement(769..2484)
                     /locus_tag="PKV30_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL31085.1"
                     /translation="MIRLSELRLPLAQADAPDAALRAAAAHKLQVSESTIAGLTVFKR
                     SFDARKADLLAVYIVDVELADPAAESPLLTRFDADPHVQPTPDMAWQPPVTAPADRSC
                     ERPVVIGFGPCGIFAALALAQMGLKPIVLERGKPVRERTQDTWGLWRRGELNPESNVQ
                     FGEGGAGLFSDGKLYSQVRDPRHLGRKVMQEFVAAGAPEEILYVAHPHVGTFKLVKVV
                     ENLRAEIVRLGGEIRFQQRVVDLLFKDELPALVLQALAVENLQNGERYELPTRHAVLA
                     LGHSSRDTFEALYRRGVDMVAKPFSIGVRIEHPQGVIDRARWGRHAGHPLLGAADYKL
                     VHHASNGRAVYSFCMCPGGTVVAATSEPGRVVTNGMSQYSRNERNANAGLVVGIEPSD
                     FPRDAAAFVEALGESFGADVLPADQTHPLAGIVLQRQLESGAYQLGGASYHAPAQRVG
                     DFLAQRPSAAPGAVEPSYKPGVHWTDLHAALPAFATAAMSEALPVFGRKIKGYDMADA
                     VMTGVETRTSSPVHIRRGDDLQSLNVRGLYPAGEGAGYAGGILSAGIDGIKVGEAVAL
                     SVLQG"
BASE COUNT          382 a          864 c          866 g          420 t
ORIGIN      
        1 gtgggtgctt tgtaaggaag ttgcttctgt attgatagct atcagcgccc atcagtcaag
       61 cgctggggcc gaaaaagact tcctatctcc gcgacctttg gactcagctg ggcacagcgg
      121 cgcatgttga gtgatgtcac gccgttctcc tcagggtttg gtggcgcgtc tcagagtccg
      181 gccatcgcgc aggcatttgc ccatgccgcg ctcgatccag ccggtgcctg caagcagccc
      241 gcggctggcg tggtggatgt ggcggtcagc ctgaccggcc ccgccagcct gacgcccggc
      301 acacccgcaa actacttgct gcgggtggcc caccccggcg tggtggcgag caccgcgatc
      361 acggtgaccc tcacattgcc gacgggtgtg accgcgaccg gtactccgcc tgttggctgc
      421 accttcagtt ccgcaaacac catcgtgacg tgtcagttgc ccgacctgtc agttgccggg
      481 agctccaact tatcgatcca gctggtggcc gcagcaggcg gtgcgggggg caacgcccag
      541 tcgtcggtgc cggcccagac cggcgaagtc aacacggcca acaacagtgc ggctttggcg
      601 atcgccattg gcgccgcgcc gccgtcaccg accgccgtgc cgacgctcga tgtcggggcg
      661 ctgtttgcgc tgggcggctt gctgccgctg gtggctgcgc gcggccgtcg ccaaaactga
      721 agccaaacct ccatccgtgg cggggctctt gggcagcgcc tgcgagcctt agccctgcag
      781 cacactcagc gccaccgctt cgccgacctt gatgccgtcg atgccggccg acagaatgcc
      841 gccggcgtag cccgcgcctt cgccggccgg gtacaggccg cgcacgttca ggctttgcaa
      901 atcgtcgcca cgacggatgt gaacgggcga cgaggtgcgc gtctcgaccc ccgtcatcac
      961 cgcatcggcc atgtcgtagc ccttgatctt gcggccgaac accggcagcg cctcgctcat
     1021 ggcggccgtg gcgaaggcgg gcagggcggc gtgcagatcg gtccagtgca cgccgggctt
     1081 gtatgagggc tcgacggcgc ccggcgcggc gctcggccgc tgcgccagaa agtcgcccac
     1141 gcgctgcgcc ggggcgtggt agctggcgcc gcccaactgg tacgccccgc tttccagctg
     1201 gcgctgaagc acgatgccgg ccagcgggtg cgtctggtcg gcgggcagca catcggcgcc
     1261 aaacgattcg cccagcgcct cgacgaacgc cgccgcatcg cgcggaaagt cgctcggctc
     1321 gatgcccacc accaggcccg cattggcgtt gcgttcgttg cggctgtact ggctcatacc
     1381 gttggtcacc acgcggcccg gctcgctggt ggccgcgacc accgtgccgc cggggcacat
     1441 gcaaaagctg tagacggcgc ggccgttcga tgcgtggtgc accagcttgt aatcggccgc
     1501 gcccagcagc gggtggccgg cgtggcggcc ccagcgcgcg cggtcgatca cgccttgcgg
     1561 gtgctcgatg cgcaccccaa tcgagaacgg cttggccacc atgtcgacgc cgcgccggta
     1621 cagcgcctcg aaggtgtcgc gcgacgagtg gccgagcgcc agcacggcgt ggcgcgtggg
     1681 cagctcgtag cgttcgccgt tttgaaggtt ttcgaccgcc agtgcttgca ggacaagcgc
     1741 gggcagctca tctttaaata gcaaatcgac cacacgctgc tgaaagcgaa tttcgccgcc
     1801 cagccgcacg atctcggccc gcaggttctc caccaccttc accagcttga aggtgccgac
     1861 gtgcgggtgg gcgacgtaca ggatttcttc cggcgcgcct gcggcgacga attcctgcat
     1921 caccttgcgc cccagatggc gcgggtcgcg cacctggctg tagagcttgc cgtcgctgaa
     1981 caatcccgcg ccgccttcgc cgaactgcac gttgctttca ggattcagct cgccgcgccg
     2041 ccacaggccc caggtgtcct gcgtgcgctc gcgcaccggc ttgccgcgct ccagcacgat
     2101 gggtttcaag cccatctgcg ccagcgccag cgcggcaaag atgccgcagg ggccgaagcc
     2161 gatgacgacc gggcgttcgc acgaccgatc ggccggcgcg gtgacgggcg gctgccaggc
     2221 catgtcgggc gtgggctgca cgtgcgggtc ggcgtcgaag cgcgtgagca gcgggctttc
     2281 ggcggccggg tcggccagtt cgacgtcgac gatgtaaacc gccagcagat cggccttgcg
     2341 ggcgtcaaag ctgcgcttga agaccgtgag gcctgcgatg gtcgattcag acacttgcag
     2401 cttgtgcgcc gcggcggcgc gcaaggcagc gtcgggcgcg tcggcctgcg cgagtggcag
     2461 gcggagttcg gacaggcgga tcatggtcgg cggtcttgtg gcgatcaagc agaagccgca
     2521 gatggtagcg cc
//
LOCUS       DAOMVO010000347         2519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12276_length_2519_cov_14.563718, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000347 DAOMVO010000000
VERSION     DAOMVO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12276_length_2519_cov_14.563718"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PKV30_12590"
     CDS             <1..161
                     /locus_tag="PKV30_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRL31086.1"
                     /translation="FREPIIDYVVVHELAHLREMNHSPRFWQHVENVLPDYAERRGAL
                     KDEAVPRW"
     gene            591..>1183
                     /locus_tag="PKV30_12595"
     CDS             591..>1183
                     /locus_tag="PKV30_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HRL31087.1"
                     /translation="MTTTELLVSKTELAATRVRQAPAAPLQSGQARARVDRFALTSNN
                     ITYAAFGDAMHYWDFFPVQGDDAQGGGSVPVWGFGTVVESACTEVPVGERVYGYWPMA
                     SHAVLTPARVTPSGWIDGAAHRAPLPPIYNQYERSSADPFYRADTEDLQALLRPLFAT
                     SWLIDDFMADSDFFGARTLLLSSASSKTAYATAWCLA"
     assembly_gap    1184..1283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1284..1502
                     /locus_tag="PKV30_12600"
     CDS             <1284..1502
                     /locus_tag="PKV30_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 domain-containing protein"
                     /protein_id="HRL31088.1"
                     /translation="AQGKKRASEWGSAVFGARLLGGWNGFLKTVLDQGWVQVTHAQGP
                     AAAQAAYAQVLGGRNDPRLGQMVSLAAE"
     gene            complement(1507..2277)
                     /locus_tag="PKV30_12605"
     CDS             complement(1507..2277)
                     /locus_tag="PKV30_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL31089.1"
                     /translation="MDTAELFSLKGRSALITGGSRGIGRMIAEGFLRAGARVYISARK
                     ADACDATAAELSVIGPCLSLPADVSTQGGVQQLVAAYQQHEQSLDILVNNAGAAWGAP
                     YDEFPESGWDKVVDLNLKAPFFLTQALTPLLKKAATDHLAKVIHIASIDGVSVNPQET
                     YSYAASKAGLIHLTRRMALKLAPERIAVSAIAPGAFASTMNKDARDHGDEVAQRIPLG
                     RIGTPEDMAGAAIFLASRAGDYVVGSTLIVDGGVTWAR"
     gene            complement(2362..>2519)
                     /locus_tag="PKV30_12610"
     CDS             complement(2362..>2519)
                     /locus_tag="PKV30_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL31090.1"
                     /translation="DVLPVLFKRPLDLHQTTFALGEAVAHLHRLWHAGQLRRERDAQG
                     IWRFAPT"
BASE COUNT          409 a          783 c          818 g          409 t
ORIGIN      
        1 actttcgtga acccatcatc gactacgtgg tggtgcacga gctggcccac ctgcgtgaga
       61 tgaaccacag cccgcgcttc tggcaacacg tcgaaaacgt gctgcccgac tacgccgagc
      121 ggcgcggtgc gctgaaggac gaggcggtgc cgcgctggtg aggctgaagg ccgaataccg
      181 gacgcgaagg acgcaaagga tccgcgaagg acgcaaaaaa actgcttgat gagcaagaac
      241 tctcagacgg cgcagcgtcc ggctgttact ccctctccct ttgggagcgg gttggggtga
      301 gggcccccgc tgattggcgt aagcggtgtt gcccccatcc cagccttccc ccagcggggg
      361 aaggggccaa gcccgtcaat cgctcggaaa cttcagtctg agacttcttg ccaatcaaga
      421 aaaagagagc tgggtgtcct cgtggcgcac tgagttgcag cggcgttgat caaaacggtc
      481 cggccgtcaa tcaccttggt gtccgcgtgc agcgagcgcg gggcgctatg ttttcatgaa
      541 ttctgtattt ttcgcgccct tcgcgtccgg ctgttttcaa ccttcacacc atgaccacca
      601 ccgaactgct cgtcagcaag accgagctcg ccgccacccg cgtgcgccag gcgcccgccg
      661 cgcctttgca atccggccag gcgcgcgcgc gtgtggaccg cttcgccctt acgtccaaca
      721 acatcaccta cgccgcgttt ggcgacgcca tgcactactg ggatttcttt ccggtgcaag
      781 gcgacgacgc ccagggcggg ggcagcgtgc cggtgtgggg ctttggcacg gtggtcgaat
      841 cggcctgcac cgaggtgccg gtgggcgagc gcgtgtacgg ctattggccg atggcgagcc
      901 acgccgtgct gacgcccgcg cgcgtcacgc ccagcggctg gatcgatggc gcggcgcacc
      961 gcgcgccgct gccgcccatc tacaaccagt acgagcgcag cagcgccgac ccgttctacc
     1021 gcgccgacac cgaagacctg caagcgctgc tgcgcccgct gtttgccacc tcgtggctga
     1081 tcgacgactt catggccgac agcgactttt tcggcgcgcg cacgctgctg ctgtccagcg
     1141 cgtccagcaa gacggcctat gccaccgcgt ggtgcctggc gcannnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnngcgcagg gcaagaagcg ggcgagcgaa tggggatcgg
     1321 cggtgttcgg cgcacggctg ctgggcggct ggaacggttt cctgaaaacc gtgctggacc
     1381 aaggctgggt gcaggtgact cacgcccaag ggccggcggc ggcgcaggcc gcctatgcgc
     1441 aagtgctggg cgggcgcaac gacccgcgct tgggccagat ggtcagcctg gcggccgaat
     1501 gagctttcag cgcgcccagg tcacgccgcc atcgacgatc agcgtcgagc cgaccacgta
     1561 gtcgcccgcg cgcgaggcca ggaagatcgc cgcgccggcc atgtcctcgg gcgtgccgat
     1621 gcggccgagc ggaatgcgct gcgccacttc gtcgccatgg tcgcgcgcgt ccttgttcat
     1681 ggtcgaggca aaggcgccgg gggcgatggc gctgacggcg atgcgctcgg gcgccagttt
     1741 cagcgccatg cggcgcgtga ggtgaatcag gccggccttg ctggcggcgt atgaatacgt
     1801 ctcctgcggg ttgaccgaga cgccatcgat ggacgctatg tggatcacct tggccaggtg
     1861 gtcggtggcg gctttcttga gcagcggggt gagcgcctgg gtgaggaaga acggcgcctt
     1921 caggttcaga tcgaccacct tgtcccagcc gctctccgga aactcgtcgt agggcgcacc
     1981 ccaggcggcg ccggcgttgt tgaccaggat gtcgagcgac tgttcatgct gctggtaagc
     2041 ggccaccagc tgctgcacgc cgccctgcgt ggacacatcg gccggcagcg acaagcaagg
     2101 accaatgacc gacagctcgg ccgccgtggc gtcgcacgcg tcggccttgc gcgccgagat
     2161 gtagacgcgc gcgccggcac gcagaaagcc ttcggcgatc atgcggccga tgccgcgcga
     2221 accgccggtg atgagggcgc tgcggccctt gagcgaaaac agttcggcgg tgtcgatcat
     2281 ggtgacgtct cctgcaatat taatttgata gctgcttgcg cttgctcatc aagcgctggc
     2341 ggcctatttg accttgagaa actaagttgg cgcaaagcgc cagatgcctt gcgcatcgcg
     2401 ctcgcgccgc agttgccccg cgtgccacag ccggtgcaaa tgcgccaccg cctcgcccag
     2461 cgcaaaggtg gtctggtgca ggtcgagcgg gcgcttgaac agcacgggca gcacgtcgt
//
LOCUS       DAOMVO010000348         2505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12370_length_2505_cov_19.644898, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000348 DAOMVO010000000
VERSION     DAOMVO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12370_length_2505_cov_19.644898"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKV30_12615"
     CDS             complement(<1..782)
                     /locus_tag="PKV30_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRL31091.1"
                     /translation="MQRRHVIALAAAVASLTAGTAFAQAYPNKPIRLIVPFAAGGTTD
                     IIARVISEPLGKALGQPVVVDNKGGAGGVIGAQEAARAAPDGYTISMATVSTTAANPA
                     INPKTGYDPVADFVPIINIAATPNLIAVHPSFAAKDHKALVELLKSQPGKFSYASSGT
                     GGIGHLQTELWKSLTGVFVTHIPYRGAGPALNDVVAGQVPIIFDNVPSSLPFIKSGKL
                     VPVVVAAPERLKDLPNTPTFKEVGLEPVNRMAYYGLLASKGTP"
     gene            949..2499
                     /locus_tag="PKV30_12620"
     CDS             949..2499
                     /locus_tag="PKV30_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRL31092.1"
                     /translation="MTNPTAPAPRRTLVDRFLDGVEKIGNKLPDPVMIFIAICALVLI
                     ASWLAAAAGVSVTNPATGKTVAAVNLLSADGLRRILSEAVKNFGAFPALGLVLVVMLG
                     IGVAEKTGYFEVAMRRVIERAPQAMIIPVIIFTAVLGNVAGDAAPIVLPPLAAMVFLR
                     LGLHPVAGVAMAYAAALGGFAANLILGMSDALVLAFTAPAAALVDPSVETNTAMNYYF
                     IAASTFLLTAVVWVVATRVGIPRLGRYDNPEFVSQEQPALTPQEIKALRAANWSLLAV
                     VGVLLALSTPSNGLLRNATTGSLIQDSPLMNGIGLIITLFFFVPGLVYGLVAGKIRSG
                     GDLAGMMTSSMASMGGFIVIVFFAAQMLAFFNWSNLGTIIAVKGAELLQNQHGMVLIV
                     GVVILSSIVNIFIGSASAKWAILAPIFVPMMMLLGFHPAFTQMIYRVGDSITNPITPM
                     LPYFPLVLGLAQKYDRKMGIGTLIAALLPYSIAIGVCWTIFLLVWFWLGWPVGPGGPI
                     YLNTPVGG"
BASE COUNT          373 a          793 c          888 g          451 t
ORIGIN      
        1 ggcgtgccct tggaggccag caggccgtag taggccatgc ggttcaccgg ctccaggccc
       61 acctccttga aggtgggggt gttgggcagg tccttcaggc gctcgggcgc ggccaccacc
      121 acgggcacca gcttgccgct cttgatgaag ggcagcgacg agggcacgtt gtcgaagatg
      181 atcggcacct ggccggccac cacgtcgttc agcgccgggc cggcgccgcg gtaggggatg
      241 tgggtgacga acacgccggt caggcttttc cacagctcgg tctgcaggtg gccgatgccg
      301 ccggtgcccg acgaggcgta gctgaacttg cccggctggc ttttcagcag ctcgaccagg
      361 gccttgtggt ccttggcggc aaagctgggg tgcaccgcga tcaggttggg cgtggcggcg
      421 atgttgatga tgggcacgaa gtcggccacc gggtcgtagc cggtcttggg gttgatggcc
      481 gggttggccg cggtggtgga caccgtggcc atgctgatgg tgtagccgtc cggcgcggcg
      541 cgcgcggcct cctgcgcgcc gatgacgccg ccggcgccgc ccttgttgtc gaccaccacg
      601 ggctggccca gggccttgcc cagcggctcg gagatcacgc gcgcgatgat gtcggtggtg
      661 ccgccggcag caaagggcac gatcaggcga atcggcttgt tgggataggc ctgggcaaag
      721 gcggtgccgg ctgtcaggct ggcgacggcg gcagccaatg cgatcacgtg gcgacgttgc
      781 ataagggcta aactcctgag aaattgaatg aacctcttga ttccgtgcaa tgcggctgag
      841 gcgggcaatc aacaagaata cacacataca acgccagttc gcggcccgga gcgacgggga
      901 tttcccctag cctgtgcgca ctggcgaagc aacaagaaga gagctgccat gaccaacccg
      961 accgccccgg caccgcgccg caccctggtg gaccgctttc tcgacggcgt cgagaagatc
     1021 ggcaacaagc tgcccgatcc ggtgatgatc ttcatcgcca tctgcgcgct ggtattgatc
     1081 gcctcgtggc tggcggcggc ggcgggcgtg tcggtcacca acccggccac gggcaagacg
     1141 gtggctgcgg tcaacctgct cagcgccgac gggctgcgcc gcatcctgtc ggaggcggtg
     1201 aagaacttcg gcgccttccc ggcgctgggc ctggtgctgg tggtgatgct gggcatcggc
     1261 gtggccgaga agacgggcta cttcgaagtg gccatgcgcc gtgtgatcga gcgcgcgccg
     1321 caggccatga tcatcccggt gatcatcttc accgccgtgc tgggcaacgt ggcgggcgat
     1381 gcggcgccca tcgtgctgcc gccgctggcg gccatggtgt ttctgcggct ggggctgcac
     1441 ccggtggcgg gcgtggccat ggcgtatgcg gcggcgctgg ggggctttgc ggccaacctg
     1501 atcctgggca tgtcggacgc gctggtgctg gccttcaccg cgccggcggc ggcgctggtc
     1561 gacccgagcg tggagaccaa cacggcgatg aactactact tcatcgccgc ctccaccttc
     1621 ttgctgacgg cggtggtgtg ggtggtggcc acgcgcgtgg gcattccgcg cctggggcgc
     1681 tacgacaacc ccgagttcgt ctcgcaagag caacccgcgc tgacgccgca ggagatcaag
     1741 gcgctgcgcg cggccaactg gtcgctgctg gcggtggtgg gcgtgctgct ggcgctcagc
     1801 actccgtcca acggcctgct gcgcaacgcc accaccggca gcctgatcca ggattcgcca
     1861 ctgatgaacg gcatcggcct gatcatcacg ctgttcttct tcgtgccggg gctggtgtac
     1921 ggcctggtgg ccggcaagat caggagcggc ggcgacctgg cggggatgat gaccagttcg
     1981 atggcgtcga tgggcggctt catcgtcatc gtgttcttcg ccgcgcagat gctggccttc
     2041 ttcaactgga gcaacctggg caccatcatc gcggtgaagg gcgccgaact gctgcagaac
     2101 cagcacggca tggtgctgat cgtcggcgtg gtcatcctgt cgtccatcgt caacatcttc
     2161 atcggcagcg cctcggccaa gtgggccatc ctggcgccga tcttcgtgcc catgatgatg
     2221 ctgctgggct ttcacccggc gttcacgcag atgatctacc gcgtgggcga ttccatcacc
     2281 aaccccatca cgccgatgct gccgtacttt ccgctggtgc tggggctggc gcagaagtac
     2341 gacaggaaga tgggcatcgg cacgctgatc gccgcgctgc tgccctactc catcgccatc
     2401 ggcgtgtgct ggacgatctt cctgctggtg tggttctggc tcggctggcc ggtcggcccc
     2461 ggcggcccga tctacctgaa cacgcccgtg ggcggctgag cggcg
//
LOCUS       DAOMVO010000349         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12467_length_2492_cov_11.925728, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000349 DAOMVO010000000
VERSION     DAOMVO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12467_length_2492_cov_11.925728"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PKV30_12625"
     CDS             <1..822
                     /locus_tag="PKV30_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL31093.1"
                     /translation="FARPTHSYLGLSPGLDFETRIIAKPNIAAVLRRELAAPGYRPSP
                     LVIGAATDCYQPAERDLRLTRALIEVLGESRHPFSIITKSSAVERDLDLIAPLAARGL
                     AAVYVTITTLDAALARQLEPRAATPARRLRTLRTLAVAGVPTGVSVAPQIPFINNDME
                     QVLQAARDAGAERAFYTVLRLPWEVAPLFQEWLAAHFPDRAARVMARVRDMRGGRDYQ
                     AQFGRRMQGEGVWADLLAQRFAAACTRLGLNGERRGVLDCSQFRPPALNGQQSLF"
     gene            complement(1050..>2492)
                     /locus_tag="PKV30_12630"
     CDS             complement(1050..>2492)
                     /locus_tag="PKV30_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HRL31094.1"
                     /translation="RWTLKPVITLFLLAAAGGAYFMLSYGVVIDRSMMVNVLLTDGRE
                     ARELLSLRMLGALLALGVLPAALLWRARIAWAPPLRQLRQNAIVFAASCAVLASAGGL
                     FFQDLSATMRNHTQLRYLVNPLNSFYALAIVGRQPVQRSGVAVLPLGEDAHLPALAAG
                     AKPPLMVMVVGETARADHMGLNGYARDTTPRLASEGVASLRNVWSCGTNTAASVPCMF
                     SNLGREAFENRPANTEGLADVLQRAGYAVLWLDNQVGGCKGVCDRVPKVDYRALQAAA
                     PELCQGDECFDEVMLRGLDERIARLPAERRARGVVVFMHQMGSHGPAYYLRSPARFKR
                     FTPECTTNALQQCAREQVVNAYDNTIAYTDHFLASTIDWLKSREAQWAPALVYVSDHG
                     ESLGENNLYLHGLPYRFAPDVQKHVPWISWFSPQFERQSGVSTACLKAKADERLSHDD
                     YFHSALGLLNVQTRVYEAARDIGASCRAAT"
BASE COUNT          409 a          853 c          838 g          392 t
ORIGIN      
        1 tttgcgcggc cgacgcacag ctacctgggc ctgtcgccgg ggctggattt cgagacccgc
       61 atcatcgcca agcccaacat cgccgccgtg ctgcggcgag aactggcggc gcccggctat
      121 cgccccagcc cgctggtgat cggcgctgcc accgactgct atcagccggc cgagcgcgac
      181 ttgcgcctga cgcgcgcact gatcgaggtg ctgggcgaat cgcggcaccc gttttccatc
      241 attaccaaat ccagcgcggt ggagcgcgat ctcgacctga tcgccccgct ggccgcgcgc
      301 gggctggcgg cggtgtacgt gaccatcacc acgctggatg cggcgttggc gcgccagctg
      361 gagccgcgcg ccgccacgcc cgcgcggcgc ctgcgcaccc tgcgcacgct ggcggtggcc
      421 ggcgtgccca cgggcgtcag cgtggcgccg cagattccct tcatcaacaa cgacatggag
      481 caggtgctgc aagccgcgcg cgacgccggc gccgaacgcg ccttctacac cgtgctgcgc
      541 ctgccgtggg aagtggcgcc gctgtttcaa gaatggctgg cggcgcattt ccccgaccgc
      601 gccgcgcgcg tgatggcccg cgtgcgcgac atgcgcggcg ggcgcgacta ccaggcgcag
      661 ttcggacgcc gcatgcaggg cgagggcgtc tgggccgatt tgctggcgca gcgatttgcc
      721 gctgcgtgca cccgcctggg gctgaacggc gagcgccgcg gcgtgcttga ctgcagccag
      781 tttcggccac cggcgctgaa cgggcagcaa agcctgtttt gaagtgttga cagcgacagc
      841 aagcccccga aaacgaccca acacccgttc gtcctgagct tgtcgaagga cttggtagcc
      901 cgtggatcgg cttcgacaag cctgtcatgg gcttgccgaa tggctcagcc cgaacggatg
      961 gtgtgaggtg cttccgagga acgttttacg gttttttggc cgccagcgct tgcctggcaa
     1021 gcgcgagcag ctatcgatac cgtagcgatt caagtcgccg cgcgacaact ggcaccaatg
     1081 tcgcgcgccg cttcatacac ccgcgtctgc acgttcagca gcccgagcgc gctgtgaaag
     1141 tagtcgtcgt gcgacaagcg ctcatccgcc ttcgctttca ggcacgccgt ggacacgccg
     1201 ctctggcgct cgaactgggg cgagaaccag ctgatccacg gcacgtgttt ttgcacgtcg
     1261 ggcgcaaagc ggtagggcag gccgtgcagg tacaggttgt tctcgcccag cgattcgccg
     1321 tggtctgaca cgtagaccag ggccggcgcc cactgcgcct cgcgtgattt cagccagtcg
     1381 atggtcgagg ccagaaagtg gtcggtgtag gcaatggtgt tgtcgtaggc attcaccacc
     1441 tgttcgcgcg cgcactgctg cagcgcgttg gtggtgcatt cgggcgtgaa gcgcttgaag
     1501 cgcgcgggcg agcgcaggta gtacgccggg ccgtggctgc ccatctggtg catgaagacg
     1561 accacgccgc gcgcgcgccg ctcggcgggc aggcgggcaa tgcgttcatc gagcccgcgc
     1621 agcatcacct cgtcaaaaca ctcatcgccc tggcacaact cgggcgctgc cgcttgcagc
     1681 gcgcggtagt cgaccttggg cacgcggtcg cacacgccct tgcagccgcc gacctggttg
     1741 tcgagccaca gcacggcgta gccggcgcgc tgcagcacgt cggccaggcc ctcggtgttg
     1801 gccgggcggt tttcaaacgc ctcgcgcccc aggttggaga acatgcatgg caccgaggcg
     1861 gcggtattgg tgccgcacga ccacacgttg cgcaggctgg ccacgccctc tgaggccagg
     1921 cgcggcgtgg tgtcgcgcgc atagccgttc aggcccatgt ggtcggcgcg cgcggtctcg
     1981 cccaccacca tgaccatcag cggcggcttg gcgcccgccg ccagcgcggg caggtgcgcg
     2041 tcttcgccca gcggcagcac cgccaccccg ctgcgctgca ccggctggcg cccgacgatg
     2101 gccagcgcat agaacgagtt gagcgggttg accagataac gcaactgcgt gtggttgcgc
     2161 atggtggcgg ataaatcctg aaagaaaagg ccgccagcgc ttgccaatac agcgcaagaa
     2221 gctgcaaaaa caatagcgtt ctggcgcagt tgacgcagcg gcggcgccca cgcgatgcgc
     2281 gcgcgccaga gcagcgccgc cggcagcacg cccagcgcca gcaaggcgcc cagcatccgc
     2341 aggctgagca attcgcgcgc ctcgcgccca tcggtcagca gcacgttgac catcatgctg
     2401 cggtcgatca ccacgccata actcagcatg aaataggcgc cgccggccgc cgccagcaag
     2461 aacagcgtga tcaccggctt gagcgtccag cg
//
LOCUS       DAOMVO010000350         2469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12624_length_2469_cov_15.625104, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000350 DAOMVO010000000
VERSION     DAOMVO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_2469_cov_15.625104"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1496)
                     /gene="gdhA"
                     /locus_tag="PKV30_12635"
     CDS             complement(168..1496)
                     /gene="gdhA"
                     /locus_tag="PKV30_12635"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRL31095.1"
                     /translation="MNDFLRRIEERDPNQPEFLQAVREVMVSLWPFVEKNPQYREYGL
                     LERICEPDRAIQFRVAWVDDKGQTQVNRAFRVEHSMAIGPYKGGMRFHPSVNLSILKF
                     LGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMSELYRHLGANTDVPA
                     GDIGVGAREVGFMAGMMKKLSNDASCVFTGKGMSYGGSLMRPEATGYGTVYFAQEMLR
                     RKQMGFEGRSVSISGSGNVAQYAAEKSMELGAKVVTLSDSDGTLVVEGGLTDGMLKDV
                     MDFKNVQRGRLKDFAKQHKLKFEAGKNPWHVPVDIALPCATQNELDAADAKHLIANGV
                     VCVAEGANMPSTLEAVDAFLAAGTLYAPGKASNAGGVATSGLEMSQNAMRLSWTHSEV
                     DLRLHEIMKDIHGNCVRHGERSDGSINYVEGANIAGFVKIADAMLAQGVL"
     gene            complement(1686..2465)
                     /gene="hisF"
                     /locus_tag="PKV30_12640"
     CDS             complement(1686..2465)
                     /gene="hisF"
                     /locus_tag="PKV30_12640"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRL31096.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDQRDLILHIIEAVASQVFIPLTVGGGVRTVDDVRRLLNAGADKTSF
                     NSAAIANPRVIRDASAKYGSQCIVVAIDAKRRVGDDAAARGEGWDVYSHGGRKNTGLD
                     AIEWAKKMAELGAGEILLTSMDKDGTKSGFDLALTRAVADAVTVPVIASGGVGHLDHL
                     ADGIQLGGADAVLAASIFHYGEFTVAQAKQRMAERGIAVRQ"
BASE COUNT          395 a          834 c          773 g          467 t
ORIGIN      
        1 gctcgcgctt gatagacaag cgccagagcc cgatttctct tgaatcacca atcgaccgac
       61 cgcgcagcag cctgccccag atcaccacgg ccgggcttgc accggcggtc ggtgatgccc
      121 gttgaggagg tggcgaaaca tggcgaagcc cgtggagcct ggggtggtca gagcacgccc
      181 tgagccagca tcgcatccgc gatcttcacg aagccggcga tgttcgcgcc ctcgacgtag
      241 ttgatgctgc cgtcgctgcg ctcgccgtgg cgcacgcagt tgccgtggat gtccttcatg
      301 atctcgtgca ggcgcagatc gacctcgctg tgcgtccacg acaggcgcat ggcgttctgg
      361 ctcatctcca ggcccgaagt cgccacacca cccgcgttgc tggccttgcc gggcgcgtac
      421 aacgtgccgg cggccaagaa ggcatccacc gcttccagcg tgctcggcat gttggcgcct
      481 tcggccacgc agaccacgcc gttggcgatc aagtgcttgg cgtcggcggc gtccagctcg
      541 ttctgcgtgg cgcagggcag cgcaatgtcg actggcacgt gccatgggtt cttgcccgct
      601 tcgaacttca gcttgtgctg cttggcaaag tccttcaggc gaccgcgctg cacgttcttg
      661 aagtccatca cgtccttcag catgccgtcg gtcaggccgc cctcgaccac cagcgtgccg
      721 tccgaatccg acagcgtgac gaccttggcg cccagctcca tcgacttttc cgccgcgtac
      781 tgcgccacgt tgccggagcc ggaaatgctg acgctgcggc cctcgaagcc catctgcttg
      841 cggcgcaaca tctcctgcgc gaagtacacc gtgccgtagc ccgtggcctc gggccgcatc
      901 aggctgccgc cgtagctcat gcccttgccc gtgaacacgc agctggcgtc gttgctcagc
      961 tttttcatca tgccggccat gaagccaacc tcgcgcgcgc caacgccgat gtcgcccgcc
     1021 ggcacgtcgg tgttggcgcc cagatggcgg tacagctcgc tcatcagcgc ctgacaaaag
     1081 cgcatcacct cgccttcgct cttgcccttg gggtcaaagt ccgatccgcc cttgccgccg
     1141 cccatgggca gcgtggtcag cgcgttcttg aaggtttgct cgaagccgag gaatttcaaa
     1201 atcgacaggt tgacgctggg atgaaaacgc atgccgccct tgtacgggcc gatggccatg
     1261 ctgtgttcga cgcgaaaggc gcggttgacc tgcgtctggc ccttgtcatc aacccacgcg
     1321 acgcggaact ggatcgcgcg gtcgggctcg cagatgcgct cgagcaagcc gtattcgcgg
     1381 tattgcgggt tcttttcgac gaacggccac agcgacacca tgacttcgcg cacggcctgc
     1441 aaaaactcgg gctggttcgg gtcgcgctct tcgatgcggc gcagaaaatc gttcatgctg
     1501 gcggcggtct tcaatcgaaa ctccttgggt tgactcggcg tgcccagcga tggtgcacga
     1561 aaacgccctc gtttccctga caggaaacca ggcgccgacg ctttgttttt gatagctggg
     1621 ggcgctgtct ggcggatggc cagggcgcta ttttcgcgca aaaatggccc caatcctgtg
     1681 cacgcttact gacgcaccgc aatcccgcgc tcggccatgc gctgcttggc ctgggccacg
     1741 gtgaactcgc cgtaatgaaa gatgctggcg gccagcaccg cgtcggcgcc gcccagctga
     1801 atgccgtcgg ccaggtggtc gaggtggccc acgccgccgg aggcgatcac cggcaccgtc
     1861 acggcatcgg ccacggcgcg cgtcagtgcc agatcgaacc cactcttggt gccatccttg
     1921 tccatgctgg tgagcagaat ctcgccggcg cccagctcgg ccatcttctt ggcccactca
     1981 atggcgtcca agcctgtgtt cttgcgcccg ccgtggctgt aaacgtccca gccctcgccg
     2041 cgcgcggccg cgtcgtcgcc cacgcggcgc ttggcgtcaa tcgccaccac gatgcactgc
     2101 gagccgtatt tggccgacgc atcgcggatg acccgcgggt tggcgatggc ggccgaattg
     2161 aagctggtct tgtcggcgcc cgcattgagc agccgccgga cgtcatccac cgtgcgcacg
     2221 ccgccgccca cggtgagggg gatgaacacc tgcgacgcca ccgcctcgat gatgtgcagg
     2281 atcaggtcgc gctggtcgct ggtggcggtg atgtcgagaa aggtgagttc gtccgcgccc
     2341 tgctcgttgt agcgcgcggc gatttcgacc ggatcgccag cgtcgcgcag ctcgacgaaa
     2401 ttgacgccct tgacgacgcg gccgccggtc acgtccaggc aggggatgat gcgtttggca
     2461 agcatggtt
//
LOCUS       DAOMVO010000351         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12699_length_2458_cov_14.124844, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000351 DAOMVO010000000
VERSION     DAOMVO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12699_length_2458_cov_14.124844"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKV30_12645"
     CDS             complement(<1..527)
                     /locus_tag="PKV30_12645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRL31097.1"
                     /translation="MSAPPHPFESLTPDFVMDALASLGLVGDGRLMALNSYENRVYLA
                     HLEPGTPLADAHAAVVLKFYRPGRWSAAQIEEEHAFAHELMAAEVPVVGPLRIDGRTL
                     HDHGGFLFSVSPRRGGRLPEPGDMETLEWIGRFLARLHTVGARQPFAQRPAVDLASYG
                     VEPRDWLLNHGALPL"
     gene            565..1035
                     /locus_tag="PKV30_12650"
     CDS             565..1035
                     /locus_tag="PKV30_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRL31098.1"
                     /translation="MTAPQYWLMKSEPDECSIDDALAAPNQTVPWIGVRNYQARNFMR
                     DDMHVGDGVLFYHSSCAEPGIAGIARVASGARVDPTQFEPGSPYYDGKSKPDAPRWLL
                     VDVQALCKTPVLGLAGMRDLPALAEMRVLQRGNRLSVTPVTAGEWRAIAALLER"
     gene            1056..1376
                     /locus_tag="PKV30_12655"
     CDS             1056..1376
                     /locus_tag="PKV30_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31099.1"
                     /translation="MDNTPRQPIPLYVKAVAALMTLVVSGLGLLAFFSRYAPPRWTRF
                     GYMAALEGSSAQTFGLALFFYGLIPLMFFARSAKQAGWIGAVVATLGTLSLVMGLIVF
                     RSSI"
     gene            complement(1491..>2458)
                     /locus_tag="PKV30_12660"
     CDS             complement(1491..>2458)
                     /locus_tag="PKV30_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL31100.1"
                     /translation="GGAGLNYVCYGLVAREVERVDSGYRSMMSVQSSLVMVPINEFGT
                     EAQKKKYLPKLATGEWIGCFGLTEPNHGSDPGSMITRAKKVDGGYSLSGAKMWITNSP
                     IADVFVVWAKDDGGQIRGFILEKGWKGLSAPAVHGKVGLRASITGEIVMDEVFCPEEN
                     AFPDVRGLKGPFTCLNSARYGIAWGALGAAEDCYNRARQYTLDRIQFGRPLAANQLIQ
                     KKLADMLTDISLGLQGCLRLGRMKDEGIAPVELTSILKRNSCGKALDIARQARDMMGG
                     NGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAITGIAAFAN"
BASE COUNT          394 a          795 c          807 g          462 t
ORIGIN      
        1 tccagcggca gcgcgccgtg gttcagcagc cagtcgcgcg gctcgacgcc gtagctggcc
       61 aggtccaccg ccggccgctg cgcaaacggc tggcgcgcgc ccacggtgtg cagccgggcg
      121 agaaagcggc cgatccattc gagcgtctcc atgtcgcccg gttcgggaag gcgcccgccg
      181 cgccgcgggc tgacgctgaa cagaaagccg ccgtggtcgt gcaacgtgcg cccatcgatg
      241 cgcagcgggc cgaccaccgg cacctcggcc gccatcaact cgtgcgcaaa ggcgtgttct
      301 tcctcgatct gcgccgcgct ccagcgcccg gggcggtaga acttgagcac cacggccgca
      361 tgcgcatcgg ccagcggcgt gccgggttcc aggtgcgcca ggtagacgcg gttctcgtac
      421 gaattcagcg ccatcagccg gccgtcgccc accaggccga ggctggccag cgcgtccatc
      481 acgaagtcgg gcgtgaggct ttcaaaaggg tggggcggag cggacatgcg cggcattgtc
      541 gctggccgcg ccacaatggc cgccatgacc gcaccgcaat actggctgat gaaatccgag
      601 ccggacgaat gctcgatcga cgatgcgctg gccgcaccca accagaccgt gccgtggatc
      661 ggcgtgcgca actatcaggc gcgcaacttc atgcgcgatg acatgcacgt gggcgacggc
      721 gtgctgttct atcactccag ttgcgctgag ccgggcattg cgggcatcgc gcgcgtggcg
      781 tcgggggcgc gggtcgatcc gacgcagttc gagcccggtt cgccgtatta cgacggcaaa
      841 tcaaagcccg atgcgccgcg ttggctgctg gtggacgtgc aggcgctgtg caagacgccc
      901 gtactggggt tggcggggat gcgtgatctg cccgcgctgg ctgagatgcg ggtgttgcag
      961 cggggaaatc gcttgtcggt cacgccggtg acggcgggcg agtggcgggc gatcgcagcg
     1021 ctgctggagc ggtagaaaaa tatcggacaa ccgtcatgga caacacaccc cgtcagccca
     1081 tcccgctcta cgtcaaagcc gtcgctgcgt tgatgacctt ggttgtatcg ggtttgggcc
     1141 tcttggcgtt tttttctcgc tacgcaccac cgcgctggac ccggtttgga tatatggcgg
     1201 cgcttgaagg gagttcggcg caaacctttg gcctcgccct ctttttttat gggctgatcc
     1261 ccttgatgtt cttcgcgcgc tctgcaaaac aggcggggtg gatcggtgcg gtggtcgcga
     1321 cgctcggcac gctcagtctg gtgatgggtc tcatcgtctt ccgctcgtcg atttgagcgc
     1381 tggcccatcg gtcaggttga cctccgctgc gtggcttcgg ctgcgcgtcc gcgcgacgct
     1441 gacgcaccag ccctcagggg cgcatttccg aagtgcgaaa acagagggca tcaattcgca
     1501 aacgccgcaa tccccgtaat cgcgcgcccc aaaatcaacg catgcacatc gtgcgtgccc
     1561 tcgtaggtgt tgaccacctc caggttcacc aggtggcgcg ccacgccgaa ttcgtcgctg
     1621 atgccgttgc cgcccatcat gtcgcgcgcc tggcgggcga tgtcgagcgc cttgccgcaa
     1681 ctgttgcgct tgaggatcga agtcagctcg acgggcgcga tgccttcgtc cttcatgcgg
     1741 cccaggcgca ggcagccttg tagccctaag ctgatgtcgg tcagcatgtc ggccagtttt
     1801 ttctggatca gctggttggc ggccagcggg cggccgaact ggatgcggtc gagcgtgtac
     1861 tggcgggcgc ggttatagca atcctctgcc gcgcccaggg caccccaggc gatgccataa
     1921 cgcgcgctgt tcaggcaggt gaacgggcct ttcaggccgc gcacgtcggg gaaggcgttt
     1981 tcttcggggc agaacacctc gtccatcacg atctcgcccg tgatgctggc gcgcaggccg
     2041 accttgccgt gcacggcggg ggcggacagg cccttccagc ccttctcaag aatgaaaccg
     2101 cgaatctgac cgccatcgtc cttggcccaa acgacgaaca cgtcggcaat ggggctgttg
     2161 gtgatccaca tcttggcgcc gctcaatgaa taaccgccat cgaccttctt ggcacgcgtg
     2221 atcatgctgc cgggatcgct gccgtggttg ggctcggtca ggccgaagca gccgatccat
     2281 tcgccggtcg ccagtttggg caaatatttt ttcttctgcg cctcggtgcc gaattcgttg
     2341 atcggcacca tgaccagcga gctttgcacg ctcatcatgc tgcgatagcc gctgtcgacc
     2401 cgttccacct cgcgcgccac caggccgtag cagacgtagt tgaggccggc gccgccgt
//
LOCUS       DAOMVO010000352         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12700_length_2458_cov_10.888473, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000352 DAOMVO010000000
VERSION     DAOMVO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12700_length_2458_cov_10.888473"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PKV30_12665"
     CDS             complement(<1..94)
                     /locus_tag="PKV30_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRL31101.1"
                     /translation="MPKKHSTPNRGYRVADQIQRDLSELIARELK"
     gene            complement(214..>2458)
                     /gene="infB"
                     /locus_tag="PKV30_12670"
     CDS             complement(214..>2458)
                     /gene="infB"
                     /locus_tag="PKV30_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRL31102.1"
                     /translation="EPAAAPAPAAEPAPAPAPAPVAAAPSAEAEDEAARAREQEESRR
                     KALAEAEAIRAMMSAPRKVLVAKKPEDRKADEAKAGAKGTLTKPAGTAAKTTTTANKE
                     VKSAKLSSSWAGDPAKKKGIPTRGDTSGGVGRGNWRSGPRGRRGDRDRDERSAPAAPV
                     EQRVLEVHVPETITVAELAHKMAVKASEVIKALMKMGQMVTINQPLDQDTAMIVVEEM
                     GHTAVIAALDDPEAFTDDEVQAAAAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVAA
                     GEAGGITQHIGAYHVETPRGVVSFLDTPGHEAFTAMRARGAQATDIVILVVAADDGVM
                     PQTKEAIKHAKAAQVPIVVAITKSDKPEANAERVKSELVGEEVVPEEFGGDSPFISVS
                     SKTGQGIDDLLEQVLLQAEVLELKAPVEAAAKGIVIEASLDKGRGPVATVLVQSGTLK
                     VGDVVLAGQTSGRVRAMLDENGRQAKSAGPSIPVEIQGLTDVPQAGDDFMVMADERRA
                     REIATYRAGKFRNTKLAKQQAAKLENMFADMTAGEVKTLPIIIKADVQGSQEALSQSL
                     LKLSTDEVKVQLVYTGVGGISESDINLAIASNAVVIGFNVRADVGARKLAEGNDIDIR
                     YYSIIYDAVDELKTAMSGMLAPEQKEEVIGTAEIRTVFVASKIGTVAGSYVTSGVVRR
                     DAHFRLLRDNVVIYTGEIESVKRMKDDVREVKEGFECGIKLKNYNDIREGDQLEIFEI
                     KEIARTL"
BASE COUNT          330 a          826 c          812 g          490 t
ORIGIN      
        1 ccttcaactc gcgcgcgatc aactccgaca gatcgcgctg gatctggtcg gcgacgcggt
       61 agccgcggtt gggggtggag tgcttcttgg gcatgaagag aatgacaaat gccggacgca
      121 gaggacgcag aacctgcgca gaaggcgcat gagattcaaa acaaaatttt gcgttctctg
      181 cggaacctct gcgtcctctg cgttcggctg ttcttacagc gtccgcgcaa tctccttgat
      241 ctcgaagatc tccaactgat caccttccct gatgtcgttg tagttcttga gcttgatacc
      301 gcactcgaag ccttccttta cttcgcgcac gtcgtccttc atgcgcttga ccgattcgat
      361 ctcgcccgtg tagatgacca cgttgtcgcg cagcaggcgg aagtgcgcat cgcgccgcac
      421 cacgccactg gtgacgtagg agccggccac ggtaccgatc ttcgaggcca cgaacacggt
      481 gcggatctcg gccgtgccga tgacttcttc cttctgctcc ggcgccagca tgcccgacat
      541 ggccgtcttc agctcgtcca cggcgtcgta gatgatgctg tagtagcgga tgtcgatgtc
      601 gttgccctcg gccagcttgc gcgcaccgac gtcggcacgc acgttgaagc cgatgaccac
      661 cgcgttggag gcgatcgcca ggttgatgtc ggactcgctg atgcctccga cgccggtgta
      721 caccagctgc accttgacct cgtcggtcga cagcttgagc agcgactgcg acagcgcttc
      781 ctgcgaaccc tgcacgtcgg ccttaatgat gatgggcagg gtcttgacct cgcccgccgt
      841 catgtcggcg aacatgttct ccagcttggc ggcctgctgc ttggccagct tggtgttgcg
      901 gaacttgccg gcacggtagg tggcgatctc gcgcgcgcgg cgctcgtcgg ccatcaccat
      961 gaagtcgtcg ccggcctgcg gcacatcggt cagaccctgg atctcgaccg gaatcgacgg
     1021 gccggcggac ttggcctgcc ggccgttttc gtccagcatg gcgcgcacgc ggccgctggt
     1081 ctggccggcc agcaccacgt cgcccacctt cagcgtgccg gactgcacca gcacggtggc
     1141 gaccgggccg cggcccttgt ccaggctggc ttcgatcacg atgcccttgg cggcggcttc
     1201 gaccggcgcc ttcagttcca gcacctcggc ctgtagcagc acctgctcca gcagatcgtc
     1261 gatgccttga ccggtcttgc tggagaccga aatgaagggc gaatcgccgc caaactcctc
     1321 gggcaccact tcttcgccga ccagctcgct cttgacgcgc tcggcgttgg cctcgggttt
     1381 gtcggacttg gtgatggcca ccacgatcgg cacctgcgcg gccttggcgt gcttgatggc
     1441 ttccttggtc tgcggcatga cgccgtcgtc ggccgccacc accaggatga cgatgtcggt
     1501 cgcctgcgcg ccacgggcac gcatggcggt aaacgcctcg tgacccggcg tgtcgaggaa
     1561 ggacacgacg ccacgcggcg tctcgacgtg gtaggcgccg atgtgctgcg tgatgccgcc
     1621 cgcttcgccc gccgccacct tggcgcggcg gatgtagtcc agcagcgagg tcttgccgtg
     1681 gtcgacgtga cccatcacgg tgacgaccgg cgggcgcggc agcgcttcgg cggctgcggc
     1741 ctgcacttcg tcgtcggtga aggcttccgg gtcgtccagc gccgcgatga cggcggtgtg
     1801 gcccatttcc tcgaccacga tcatggccgt gtcctggtcc agcggctggt tgatggtgac
     1861 catctggccc atcttcatca gcgccttgat gacctcggac gccttcaccg ccatcttgtg
     1921 cgccagttcg gccacggtaa tggtttcggg cacgtggacc tcgagcacgc gctgctcgac
     1981 cggtgcggcc ggtgcactgc gttcgtcgcg atcacgatcg ccacggcggc cgcgcgggcc
     2041 gctgcgccag ttgccgcggc ccacgccacc actggtgtcg ccacgggtcg ggatgccctt
     2101 cttcttggcc ggatcgccgg cccagctgga cgacagcttg gccgacttga cctccttgtt
     2161 ggcggtggtc gtggtcttgg cggcggtgcc ggccggcttg gtgagcgtgc ccttggcacc
     2221 ggccttggct tcgtcggcct tgcggtcttc cggtttcttg gcgaccagca ccttgcgcgg
     2281 cgcgctcatc atggcgcgga tggcctcggc ttcggccagg gccttgcggc ggctttcctc
     2341 ttgctcgcgg gcacgggcgg cctcgtcctc ggcctcggcg ctgggggcag cggccacagg
     2401 ggcgggtgcg ggggccggcg ctggttcggc ggcaggggcc ggcgccgcgg cgggctcg
//
LOCUS       DAOMVO010000353         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12798_length_2444_cov_16.802846, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000353 DAOMVO010000000
VERSION     DAOMVO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12798_length_2444_cov_16.802846"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PKV30_12675"
     CDS             complement(<1..515)
                     /locus_tag="PKV30_12675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL31103.1"
                     /translation="MTSPRTPAPDVSIVVPIYNEVDNLPDLVARIGRAMAGQPLSFEL
                     LAVDDGSTDGSRARLSELATTTPWLRPVLLARNYGQSSALQAGFDRVRGRYVVTLDAD
                     LQNEPDDIPLLLQRLETDPDVDMVSGWRKDRQDAELSRKLPSRIANKLISNATGVHLH
                     DYGCALKAYRRP"
     gene            603..2123
                     /locus_tag="PKV30_12680"
     CDS             603..2123
                     /locus_tag="PKV30_12680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL31104.1"
                     /translation="MNVFAATPGALSHRSPVAHPLAWLLALSLAHVAVRVAISPALKW
                     DEAEQMLWSQQLHAGYGAQPPLYTWLQWAVNQVLGPSVLALSFLKHALLTLAYVFMYL
                     AGRELLGPRGAWWASASMLLLPPLGWYSIRDQTHTILVTAMACAAWWLLLRIVRHRRP
                     ADFAWLGLVCGCGMLAKYSFALVAVAMVLAALSVPESRRALLSRGWWWAPIVGTLVVL
                     PHALWLLGHLHVATSETVGKMQIHAERRLGKGLLSLADDVIGTLLLWVLFALWAFRAA
                     WWRKPLTPALPWAHRLLWRYLALIVLALLGMVLVAGVTSFKGRWMVPLLCVAPLAAFA
                     ARPELQQHPRGGRYTRAIVVMALLILLAAGLRPWFSGLRGDTDELNHPAAELGLALQQ
                     AGYDGTSRIIAADHMLAGMLRTRFPRAPVDSCNTDEKEEVTPCVAGNVALAQRAGQGW
                     LLISRSDRVAPDWWAQAQGAVTPQAVQTIERPFHMVREGVAPAQYRYVWHAAGSKP"
     gene            2120..>2444
                     /locus_tag="PKV30_12685"
     CDS             2120..>2444
                     /locus_tag="PKV30_12685"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRL31105.1"
                     /translation="MSDNVTLVTGCAGFIGMHAAERLLARGERVVGIDNLNAYYDPAL
                     KRARLARLQPHAGFSFEQIDVADRAAMVELFARVKPTRVLHLAAQAGVRYSIDQPDDY
                     TDSNLL"
BASE COUNT          309 a          837 c          889 g          409 t
ORIGIN      
        1 gggcggcggt aggccttcag cgcgcagccg tagtcgtgca ggtgcacgcc ggtggcgttg
       61 gagatcagct tgttggcgat gcgggacggc agcttgcgcg agagttcagc gtcctgccga
      121 tccttgcgcc agccgctgac catgtcgacg tcggggtcgg tttccagccg ctgcagcaac
      181 agggggatgt cgtccggctc gttttgcaga tcggcatcga gcgtgaccac gtagcgcccg
      241 cgcacgcggt caaaaccggc ctgcagcgcg ctcgactggc cgtagttgcg cgccagcagc
      301 accgggcgca gccagggcgt ggtggtggcc agttcgctta agcgggcgcg gctgccgtcg
      361 gtggagccgt cgtccaccgc cagcagttcg aaagacagcg gctgccccgc catggcgcgg
      421 ccgatgcgcg ccaccaggtc gggcaggttg tccacctcgt tgtagatcgg caccacgatg
      481 gagacatcgg gcgccggtgt gcgggggctg gtcatggcgg gcgctggggc tggcgggata
      541 atggcgtcga ttgtgccttg caccgccgcc gccgctgccg tcggcgccct tccccagttt
      601 tcatgaacgt cttcgccgcc acgcccggcg ccttgtcgca ccgctcgccc gtcgcccacc
      661 cgctggcctg gctgctggcg ctgtcgctcg cgcatgtggc ggtgcgggtg gcgatttcgc
      721 cggcgctaaa gtgggacgag gccgagcaga tgctgtggtc gcagcagctg cacgcaggct
      781 acggcgcgca gccgccgctc tacacctggc tgcagtgggc ggtgaaccag gtgctgggcc
      841 ccagcgtgct ggcgctgtcc ttcctcaagc atgcgctgct gacgctggcc tacgtgttca
      901 tgtacctggc cgggcgcgag ctgctgggcc cgcgcggcgc ctggtgggcg tcggccagca
      961 tgctgctgct gccgccgctg ggctggtatt cgatccgcga ccagacgcac accattctcg
     1021 tcaccgccat ggcgtgcgcg gcctggtggc tgctgctgcg catcgtgcgg catcgaaggc
     1081 cggcggattt tgcgtggctc gggctggtgt gcggctgcgg catgctggcc aagtacagct
     1141 ttgcgctggt ggccgtcgcc atggtgctgg ccgcgctgtc ggtgcccgaa tcgcgccgcg
     1201 cgctgctgtc gcgcggctgg tggtgggcgc ccattgtcgg cacgctggtg gtgctgccgc
     1261 acgctctgtg gctgctcggg cacctgcacg tcgccaccag cgaaaccgtg ggcaagatgc
     1321 agattcatgc cgagcgccga ctcggcaagg ggctgctcag cctggcggac gacgtgatcg
     1381 gcacgctgct gttgtgggtg ctgttcgcgc tgtgggcgtt tcgcgccgcc tggtggcgca
     1441 aaccgctcac gccggcgctg ccgtgggcgc accggctgct gtggcgctat ctggcgctga
     1501 tcgtgctggc cctgctcggc atggtgctgg tggcgggcgt gaccagcttc aaggggcgct
     1561 ggatggtgcc gcttctgtgt gtggcgccgc tggccgcgtt tgccgcccgc ccggagctgc
     1621 agcagcaccc gcgcggcggg cgctacacgc gcgccatcgt ggtcatggcg ctgctgattc
     1681 tgttggccgc cggcctgcgc ccgtggttca gcgggctgcg cggcgacacc gatgagctga
     1741 accacccggc cgccgagctg ggcctggcgc tgcagcaggc cggctacgac ggcaccagcc
     1801 ggatcatcgc cgccgaccac atgctggccg gcatgctgcg cacgcgcttt ccgcgcgcgc
     1861 cggtcgattc gtgcaacacc gacgaaaaag aagaggtcac gccctgcgtg gcgggcaacg
     1921 tcgcgctcgc ccagcgggcc ggccagggct ggctgctgat ctcgcgcagc gaccgcgtgg
     1981 cgccagactg gtgggcgcag gcccagggcg ccgtcacccc acaagccgtg cagaccatcg
     2041 agcgaccctt tcacatggtg cgcgagggcg tggcgccggc gcagtaccgt tacgtctggc
     2101 acgctgcggg gtccaaacca tgagcgacaa cgtcaccctg gtcaccggct gcgccggctt
     2161 catcggcatg cacgccgcag aacggctgct ggcgcgcggc gagcgcgtgg tcggcatcga
     2221 caacctcaac gcctattacg acccggccct gaagcgggcg aggctggcgc gcctgcagcc
     2281 gcacgcgggc ttcagcttcg agcagatcga cgtcgccgac cgtgcggcca tggtcgagct
     2341 gtttgcacgc gtcaagccca cacgcgtgct gcacctggcc gcgcaggccg gcgtgcgcta
     2401 ctccatcgac cagcccgacg actacaccga cagcaacctg ctcg
//
LOCUS       DAOMVO010000354         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12921_length_2426_cov_14.954871, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000354 DAOMVO010000000
VERSION     DAOMVO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12921_length_2426_cov_14.954871"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1035
                     /locus_tag="PKV30_12690"
     CDS             202..1035
                     /locus_tag="PKV30_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL31106.1"
                     /translation="MLALYRFASRWPLALLHALGAALGWLTWAASPTYRRRITENARR
                     AGYRFADVRACVAQAGRMATETPRLWFGAPVPVQWRGVDQLDAAYAARKGIVFMTPHM
                     GCFEITAQAVGARYHAEHGPITVLYRPARQPALAEVIETARRREGLETAPTTLAGVRQ
                     MIKALRRGQAVGLLPDQVPPQGQGVWSPFFGEPAYTMTLAARLIQQTGATPLLVWGER
                     LPRAQGFRVHIQPFPETLSADLDTAVAQINRAMEGLIRQCPQQYLWGYGRYKKPRDAA
                     A"
     gene            1032..1895
                     /locus_tag="PKV30_12695"
     CDS             1032..1895
                     /locus_tag="PKV30_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HRL31107.1"
                     /translation="MTHPGILFMRALSHLPLGAVRGLGTLLGRLLFVLARSRRRVALI
                     NLRLAYPQWSEAERVALARRNFVYFAQAFLDRSWLWHAHPGLVRQRLRITGAVDDLTR
                     EGAVVMFAPHFYGMDAGGSAVMQQIDRPACTIYSTQPNPALDEWMQAGRQRFGDATLL
                     SRTEGVKPIVRALREGKMLYLLPDMDLGPTESVFVPFYGVPTATVPSLSRFAKLGHAR
                     VVPVVTRLTDFGYEVKVHPAWPDYPSADATADAALMNQRLQGYIDEMPEQYYWVHKRF
                     KTRPPGEASFY"
     gene            complement(2032..>2426)
                     /locus_tag="PKV30_12700"
     CDS             complement(2032..>2426)
                     /locus_tag="PKV30_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL31108.1"
                     /translation="ALALPDGAGWMTDALRAQYRSVWTGDGSRPGAGLTGGLNYYRVS
                     PLRPAREGDAAAAGVELPPEAFTVKPPTLVLWAMQDSALLPELLSGLQRWVPQMQLER
                     IERATHWVVHEQPARVAASIQRFLGDDQ"
BASE COUNT          344 a          845 c          849 g          388 t
ORIGIN      
        1 ttgtcggggt ggccttcaga gacggattcg gatgtgaaga gaaagctgtt cgccagttcc
       61 attgaataaa ctccagaggt ttgaacgttg cgttgccaaa cttctcgggg agccctggcg
      121 aacgctttag cagcactttt ttaacgtcgc cctgcaagta gttccataac tcggcgaccc
      181 gcgcatttta accaatccgt catgctggcc ttgtatcgct ttgcctcgcg ctggccgctg
      241 gcgctgctgc atgcgctggg cgcggcgctg ggctggctga cctgggctgc gtcgcccacc
      301 taccgccgcc gcatcaccga aaacgcgcgc cgcgccggct accgctttgc cgacgtgcgc
      361 gcctgcgtgg cgcaggccgg gcgcatggcg acggagacgc cgcgcctgtg gtttggcgcc
      421 ccggtgccgg tgcagtggcg gggcgtcgac cagctggacg cggcctacgc ggcgcgcaag
      481 ggcatcgtct tcatgacgcc gcacatgggc tgcttcgaga tcaccgcgca ggcggtgggc
      541 gcgcgctacc acgccgagca tgggccgatc acggtgctgt accggccggc gcgccagccg
      601 gcgctggccg aagtgatcga gaccgcgcgc cgccgcgaag ggctggagac agcgccgacc
      661 acgctggccg gcgtgcggca gatgatcaag gcgctgcggc gcggccaggc cgtgggcctg
      721 ctgcccgacc aggtgccgcc gcaagggcag ggcgtgtggt cgccgttttt tggcgagccg
      781 gcctacacca tgacgctggc ggcgcgactg atccagcaga ccggtgccac gccgctcctg
      841 gtgtggggcg agcggctgcc gcgcgcgcag gggtttcgcg tccacatcca gccctttccg
      901 gaaacgctca gtgccgacct ggacaccgcc gtggcgcaga tcaaccgcgc catggaaggg
      961 ctgatccgcc agtgccctca gcagtacctg tggggctacg ggcgctacaa gaagccacgg
     1021 gacgccgccg catgacgcac cccggcatcc tcttcatgcg cgcgctgtcg cacctgcccc
     1081 tgggcgcggt gcggggcttg ggcacgctgc tcgggcgcct gctgttcgta ctggcgcggt
     1141 cgcgccggcg ggtggcgctg atcaacctgc gcctggccta cccacagtgg agcgaagccg
     1201 agcgcgtggc gctggcgcgg cgcaacttcg tctacttcgc gcaggcgttt ctggatcgct
     1261 cgtggctctg gcatgcccac ccgggcctgg tgcgccagcg cctgcgcatc accggcgcgg
     1321 tggatgattt gacgcgcgaa ggcgcggtgg tgatgtttgc gccgcatttc tacggcatgg
     1381 acgccggcgg cagcgcggtg atgcagcaga tcgaccgccc cgcctgcacc atctattcga
     1441 cgcagcccaa cccggcgctg gacgagtgga tgcaggccgg ccgccagcgc tttggcgacg
     1501 ccacgctgct gtcgcgcacc gaaggcgtca agcccatcgt gcgcgccctg cgcgagggca
     1561 agatgctcta cctgctgccc gacatggacc tgggtccgac cgaatcggtc ttcgtgccgt
     1621 tctacggcgt gccgacggcg acggtgcctt cgctgtcgcg ctttgccaag ctcggccacg
     1681 cgcgcgtggt gccggtggtc acgcgcttga ccgactttgg ctacgaggtg aaggtgcacc
     1741 ccgcctggcc cgactacccg agcgccgacg ccaccgccga cgccgcgctg atgaaccagc
     1801 gcctgcaggg ctacatcgac gagatgcccg aacagtatta ctgggtgcac aagcgcttca
     1861 agacgcggcc gccgggcgag gcgtcgtttt actgagggat gaccggcgct ggcgcgccgt
     1921 gacctcggcc cgcggccatc gatgacgcac cctgcgggcg catgacttca atcatggcgc
     1981 ggtcgcgcgg ttatcgaagg cgaggccgag gtcatgcgcc gtcaggcgcg gtcattggtc
     2041 atcgcccaga aagcgctgaa tcgacgccgc cacccgcgcc ggttgctcgt gcaccaccca
     2101 gtgcgtggcg cgctcgatgc gctccagctg catctgcggc acccagcgct gcagaccgct
     2161 cagcagctcc ggcagcaggg cgctgtcctg catcgcccac agcaccagcg tgggcggctt
     2221 gacggtgaag gcttcgggcg gcaattcgac gccggccgct gccgcgtcgc cctcgcgtgc
     2281 cgggcgcagc ggcgagacgc ggtagtagtt cagcccgccc gtcaggcccg cgcccgggcg
     2341 gctgccgtcg cccgtccaca cggagcggta ttgcgcgcgc agggcgtcgg tcatccagcc
     2401 ggcgccgtcg ggcagcgcaa gggcct
//
LOCUS       DAOMVO010000355         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12931_length_2425_cov_12.577637, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000355 DAOMVO010000000
VERSION     DAOMVO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12931_length_2425_cov_12.577637"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKV30_12705"
     CDS             <1..344
                     /locus_tag="PKV30_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein pill,
                     pfgi-1"
                     /protein_id="HRL31109.1"
                     /translation="VSMPEDARATVGDGLRHVLKRSGYGLCQTAHAVIELYALPLPAA
                     HLHLGPMTLRDALLTLAGPAWELHADDRARQICFERPGDSAAIEPASEPPAAEAVQTF
                     PLEPSVSGGQP"
     gene            341..991
                     /locus_tag="PKV30_12710"
     CDS             341..991
                     /locus_tag="PKV30_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31110.1"
                     /translation="MNAPQASRRPAAAVVVQSLLWLWLIGLSVFVALGYQTMNDQADQ
                     ERLDSRLQRLEARSAGLVESIEAIQQRPAVATAADLKDTRQVLEARAAQVEKTLSGYA
                     AADDLQALRAEVEQIKARQTAAPVPRAAAPAQPRASGKAAAKPEPPPLPFRVVGAELR
                     AGQRSVSVAPSSADFTPDQLQVLLPGDALGPWRLQAIEGNTAVFQAGDQTRRVAIP"
     gene            1006..1731
                     /locus_tag="PKV30_12715"
     CDS             1006..1731
                     /locus_tag="PKV30_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HRL31111.1"
                     /translation="MKPSIILSALLLASAQLPAWAQQPATAPARNAQSQERPLVTRTP
                     GDQVASGWGLQPQEWTRYRELMDGPLGIYSPNLDPLSALGIEARTDEERRRYAELQVQ
                     IEARRVEKLLAYQRAYDEAWQRLNPDMQRVNLPDDKPSAGAVRGSGRMAVFVKDGCVA
                     CGQLVQRLQSSGTEFDLYMVGSRQDDARIRDWAKRAQIDAARVRSGSITLNHDGGRWL
                     SLGLPGDLPAVVREVNGQWQRQP"
     gene            1713..2303
                     /locus_tag="PKV30_12720"
     CDS             1713..2303
                     /locus_tag="PKV30_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRL31112.1"
                     /translation="MAAPTLTAPLRALVLAAGLCACAAQAQEVPPPAYQLAAQRAGIP
                     STVLYAVALQESGIRRNERIVPWPWSLNVAGQSRRYATRADACAGLQQAMRSTPHTRI
                     DAGLGQINLGYHQQRYASACDLLDPYRNLAIAAEILKEQHTPGEDWLVAIGRYHRPAG
                     GEPAARYRRSVSRHLARVQGARATAAVLAARQETSP"
     gene            2300..>2425
                     /locus_tag="PKV30_12725"
     CDS             2300..>2425
                     /locus_tag="PKV30_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HRL31113.1"
                     /translation="MTKSHLAHLAAKGLLVLLAGLPLVSRAGEPLIVVEDRGGTSA"
BASE COUNT          378 a          909 c          800 g          338 t
ORIGIN      
        1 acgtatccat gcccgaagat gcccgcgcca ccgtcggcga tgggctgcgg catgtactca
       61 agcgcagcgg ttacggcctg tgccagacgg cgcacgcggt aatcgagctg tatgcgctgc
      121 cgctgccggc ggcgcacctg cacctcggcc ccatgacctt gcgcgatgcg ctgctcacac
      181 tggctggccc ggcctgggaa ctgcacgcgg atgatcgcgc acggcagatt tgcttcgagc
      241 gccccggcga cagcgcggct atcgaacccg catccgagcc acccgccgcc gaggcggtgc
      301 agacgttccc gctggagcct tcggtttcgg gaggccagcc atgaacgccc cgcaggcttc
      361 ccggcgcccg gccgccgccg tggtggtgca gagccttcta tggctctggc tgatcggcct
      421 cagcgtcttc gtagccttgg gctaccagac catgaacgac caggccgacc aggaacggct
      481 cgattcccgc ctgcaacgcc tggaagcccg gtcggcgggc ctggtcgagt cgatcgaggc
      541 catccagcag cgtccggccg tcgcaacggc ggcagacctc aaagacaccc gccaagtctt
      601 ggaagcacgc gcagcccagg ttgagaaaac gctgagcggc tatgccgctg ccgacgacct
      661 tcaggcgctg cgcgcggagg tcgagcagat caaggcgcgc cagaccgctg cgcccgttcc
      721 gcgcgccgcc gcacccgccc agccgcgcgc atcgggcaag gccgccgcca agcccgaacc
      781 gccgccgctt ccgttccgcg tcgtcggcgc cgaattgcgc gctggccagc gcagcgtgtc
      841 cgtcgcgccg agcagcgcgg acttcacgcc cgaccagctt caggtgctgc tgccaggtga
      901 tgcgctcggc ccgtggcgct tacaggcgat tgagggcaac accgccgtgt tccaggccgg
      961 cgaccagacc cgtcgcgtgg cgattccctg accggagcac accacatgaa gccgtcgatc
     1021 atcctttccg cgctcctgct ggcatccgcc cagttgcccg cctgggcgca gcagccggcc
     1081 accgccccgg cccgcaatgc gcagagccag gagcgtccgc tggtcacccg aaccccgggc
     1141 gaccaggtag caagcgggtg gggcctgcaa ccgcaggaat ggacgcgcta tcgcgagttg
     1201 atggacgggc cgctaggcat ctactcgccc aacttggacc cgctgtccgc cctgggtatc
     1261 gaggcccgca ccgacgagga acggcggcgc tacgcagaac tgcaggtgca aatcgaagcg
     1321 cgccgcgtcg agaagctgct tgcctatcaa cgtgcctatg acgaagcctg gcagcgcctg
     1381 aaccccgaca tgcaacgggt gaacctgcct gacgacaagc ccagcgccgg cgcagtgcgc
     1441 ggcagcggcc gcatggcagt gttcgtcaag gacggctgcg tggcctgcgg acagctcgtg
     1501 cagcgcctgc aatcctcggg taccgagttc gacctgtaca tggtcggcag ccgccaggac
     1561 gacgcgcgca tccgcgactg ggccaagcgc gcacagatcg acgcggcacg ggtgcgtagc
     1621 ggcagcatca cgctcaacca cgacggcggc cgctggctgt cgctgggcct gcccggcgat
     1681 ttgcctgcgg tcgtgcgcga agtgaacggc caatggcagc gccaaccctg acggcgccgt
     1741 tgcgcgctct ggtgctcgct gctggcctct gcgcctgcgc cgcccaggcg caggaggttc
     1801 cgccaccggc ctaccagctt gccgcccagc gcgcgggcat tccctcgacg gtgctctacg
     1861 ccgtggcctt gcaggagagc ggcatccggc gcaacgagcg catcgtcccg tggccgtggt
     1921 ccctcaacgt cgccggccaa tcgcgccgct atgcgacccg cgccgacgcc tgcgccggtt
     1981 tgcagcaggc catgcgctcc acgccgcaca cgcgcatcga cgcgggcctg ggccagatca
     2041 acctcggcta ccaccagcag cgctacgcca gcgcatgcga tctgctcgac ccgtaccgca
     2101 atctcgccat cgccgccgag atcctgaaag aacagcacac ccccggcgag gactggctgg
     2161 tggcgatcgg ccgttaccac cgccccgcgg gtggcgagcc cgccgcccgc tatcggcgca
     2221 gcgtgtcccg ccacctcgcg cgcgtgcagg gcgcgcgcgc aaccgccgcg gtcctcgccg
     2281 cgcgccagga gacctcccca tgacgaaatc ccatctggcc catctcgcgg caaagggcct
     2341 gctcgtgctg ctggcgggtc tgccgctggt ctcgcgtgcc ggcgagccgc tgatcgtggt
     2401 cgaagaccgt ggcggcacgt cggcg
//
LOCUS       DAOMVO010000356         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_12995_length_2413_cov_11.329517, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000356 DAOMVO010000000
VERSION     DAOMVO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12995_length_2413_cov_11.329517"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKV30_12730"
     CDS             complement(<1..1166)
                     /locus_tag="PKV30_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRL31114.1"
                     /translation="MSIHAALHHVTHYRYDRPVQLGPQIVRLRPAPHCRSKIISYSLK
                     VEPAEHFINWQQDPFANWQARLVFPEKTREFKVTVDLVTEMAVFNPFDFFLEPEAENF
                     PFQYDPLLKEELAPYLALAPAADLFSNYLAGVDRRPQRTIDFLVGINQRLQHDINYLI
                     RMEPGVQTPEQTLQNASGSCRDSGWLLVQLLRHCGLAARFVSGYLIQLKADQKSLDGP
                     SGTEVDFTDLHAWCEVYLPGAGWIGLDPTSGLLAGEGHIPLACTPQPSGAAPIEGLVD
                     PAEVAFSHEMAVTRIHESPRVTLPYDDEQWRAILQLGDAVDLQLQEDDVRLTMGGEPT
                     FVGIDDRDGPEWNTAALGPTKRVFALELLHKLKDRYGPQGFLHLGQGKWYPGEQL"
     gene            1332..>2413
                     /locus_tag="PKV30_12735"
     CDS             1332..>2413
                     /locus_tag="PKV30_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRL31115.1"
                     /translation="MTATETACEPHVRRHALRWRGHAWAAALLGWVLGTVLQVQQPVL
                     WAAGVYIGVTVFAFALTLLAWRARPGAARALGWLLAAALAAFGTTGWRATMMLAERLT
                     PALEGRDVLLTGTVSRMPQKSEAGWRFRFDVQQAQLDGAPVPVPPRLQLAWYGSASTA
                     DEADAARPLLAELRAGDRWQFTARLKAPHGNLNPHGFDYELWLWEQGVGATGYVRSGP
                     RDVPPRRLTEGGWSIERWRQSVRDRILGDAGDAESRRFAGIVAALVTGDQNVIERADW
                     DVFRATGVAHLMSISGLHITMFAWLAAACIGWAWRRSARLGLPARWDPCQRLPAPAAA
                     LIGGVLLATGYALFSGWGVPAQRTVL"
BASE COUNT          362 a          785 c          863 g          403 t
ORIGIN      
        1 agttgctcgc cggggtacca cttgccctgg cccaggtgca aaaagccttg cggaccgtag
       61 cggtctttca gcttgtgaag cagctccagc gcaaacacgc gcttggtggg ccccagcgcg
      121 gcggtgttcc attccggccc gtcgcggtcg tcgatgccga caaaggtcgg ctcgccgccc
      181 atggtcaggc gcacgtcgtc ttcctgcagt tgcagatcga ccgcgtcacc cagttgcagg
      241 atggcgcgcc actgttcgtc gtcgtagggc agggtgacgc gcggcgattc gtggatgcgc
      301 gtgacggcca tctcgtgcga gaacgccacc tcggccgggt cgaccaaccc ctcgatcggc
      361 gccgcgcccg atggctgcgg cgtgcaggcg agcggaatgt ggccttcgcc cgccagcagg
      421 ccgctggtcg ggtccagccc gatccagccg gcgcccggca gatacacctc gcaccaggcg
      481 tgcaggtcgg tgaagtccac ctcggtgccg ctcgggccgt cgagcgactt ttgatcggcc
      541 ttcaactgaa tcagataacc cgacacgaag cgggccgcaa gtccgcaatg gcgcagcagt
      601 tgcaccagca gccagcccga atcgcggcac gagccgctgg cgttttgcag cgtctgctcg
      661 ggcgtttgca cgccgggttc catgcggatc aggtagttga tgtcgtgctg cagccgctgg
      721 ttgatgccga ccagaaagtc gatggtgcgc tgcggccggc ggtccacgcc agccaggtag
      781 ttcgaaaaca aatcggccgc tggcgcaagc gccaggtacg gtgccagctc ttctttcaat
      841 agcgggtcgt attgaaaggg gaagttctcg gcctcgggct ccagaaagaa atcaaacggg
      901 ttgaacaccg ccatctcggt caccagatcg acggtgacct tgaactcgcg cgtcttctcg
      961 gggaacacca gccgcgcctg ccagttggca aacgggtctt gctgccagtt gatgaagtgc
     1021 tcggccggtt cgaccttgag cgagtacgaa atgatcttgc tgcggcagtg cggggcaggg
     1081 cgcagccgca cgatctgcgg ccccagttgc acgggccggt cgtagcggta atgcgtgacg
     1141 tgatgaagcg cggcgtgaat ggacatgggg tctgctgcct ggccgttgtg gtggggatgg
     1201 atactggggc atcctagcaa ggcgcgcgcc aggcggtggc cgcgacgcgc tggcggaagt
     1261 gggtatctga agaaaatcgg ccgccagcgc ttgataaaca agcgcgagca gctataaaaa
     1321 caagagtatc gatgacggcg acggaaacag cctgcgaacc tcacgtgcgg cgccacgcct
     1381 tgcgctggcg cggccacgcg tgggctgcgg cgctgctggg ttgggtgctg ggcacggtgc
     1441 tgcaagtgca gcagccggtg ctgtgggcgg cgggcgtcta catcggcgtg acggttttcg
     1501 cgttcgcgct gacgctgctg gcgtggcgcg cccggccggg tgcggcgcgc gccctcggct
     1561 ggctgctggc cgccgcgctg gccgccttcg gcacgaccgg ctggcgcgcc accatgatgc
     1621 tggccgaacg cctcacgccc gcgctggaag ggcgcgacgt gctgctcacc ggcaccgtca
     1681 gccgcatgcc gcaaaagagc gaggccggct ggcgctttcg cttcgacgtg cagcaggcgc
     1741 agctcgacgg cgcgccggtg cccgtgccgc cgcgcctgca actcgcctgg tacggctcgg
     1801 ccagcacggc cgatgaggcc gacgccgcgc gcccgctgct ggccgagttg cgcgccggcg
     1861 accgctggca attcaccgcg cgtttgaagg cgccgcacgg caacctgaac ccgcacggtt
     1921 ttgactacga gctgtggttg tgggagcagg gcgtgggcgc caccggctac gtgcgcagcg
     1981 gcccgcgcga tgtgccgccg cgccgcttga ccgaaggcgg ttggtccatc gagcgctggc
     2041 gccagtcggt gcgcgaccgc attctgggcg atgcgggcga tgccgaatcg cgccgctttg
     2101 ccggcatcgt cgccgcgctg gtcacgggcg accagaacgt gatcgagcgc gcggattggg
     2161 acgtttttcg cgccaccggc gtcgcgcatt tgatgagcat ttcaggcctg cacatcacga
     2221 tgttcgcctg gctggcggcg gcctgcatcg gctgggcgtg gcgccgttcg gcgcgcctcg
     2281 gcctgccggc gcgctgggac ccatgccaac gcctgcccgc acccgcggca gcgctcatcg
     2341 gcggtgtgct gctggccacg ggctacgcgc tgttcagcgg ctggggcgtg ccggcgcagc
     2401 gcacggtgct gat
//
LOCUS       DAOMVO010000357         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13008_length_2410_cov_21.017834, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000357 DAOMVO010000000
VERSION     DAOMVO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13008_length_2410_cov_21.017834"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKV30_12740"
     CDS             complement(<1..497)
                     /locus_tag="PKV30_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRL31116.1"
                     /translation="MDLINNLSLGFSVAFTMQNLMYAFIGCFLGTLIGVLPGVGPVAT
                     IAMLLPATYALPPVAALIMLAGIYYGAQYGGSTTAILVNLPGESSSVVTVIDGYQMAR
                     KGRAGPALAAAGIGSFIAGCFGTLILAGFAVPLTEVAFKFGPAEYFSLMILGLIGAVV
                     LASGSL"
     gene            complement(513..1001)
                     /locus_tag="PKV30_12745"
     CDS             complement(513..1001)
                     /locus_tag="PKV30_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRL31117.1"
                     /translation="MRIKSQKDFFAGLLYFAIGVAFAWGATNYNIGDGARMGPGYFPL
                     LLGIVLAIIGGIVMFQAMVVETPDGAPIGKWAWKPLVFIIAANFLFGILLGGWPLLGI
                     PAMGLIIAIFVLTFVAAAAGTEFKFIEVLILAILLAALSWFAFVWGLNLQFQVWPTFI
                     TG"
     gene            complement(1220..>2410)
                     /locus_tag="PKV30_12750"
     CDS             complement(1220..>2410)
                     /locus_tag="PKV30_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRL31118.1"
                     /translation="QLDANDQGVIQVVDWLWQYAFDQRASDIHLEPRRDQGVIRFRID
                     GVLHPVYQVPPGVMNAITARVKLLGRMDVVEKRRPQDGRIKTRNPRGDEIEMRLSTLP
                     TAFGEKLVMRIFDPDTAVKDLSALGFTPHDAGRWEQLVSRPHGIILVTGPTGSGKTTT
                     LYSTLRRVATEEVNVSTIEDPIEKIDPALNQTQVQPQLDFSFAEGVRALMRQDPDIIM
                     IGEIRDLETAEMAVQAALTGHLVFSTLHTNDAPSALTRLMELGVPAYLLNATILGVLA
                     QRLVRTLCPQCKQPDDGASTELLAEAVKPWKINSGNWRPYKPVGCVDCRMTGFRGRMG
                     IYELLSVTEAFKGLVQHDVGPAPLRKQAVADGMRPLRLAGAMRAAEGLTTLPEVIAAT
                     PPLS"
BASE COUNT          456 a          785 c          773 g          396 t
ORIGIN      
        1 agcgagcccg aggccagcac cacggcgccg atcagaccca ggatcatgag cgagaagtac
       61 tcggccgggc cgaacttgaa ggccacttcg gtcagcggca cggcgaagcc ggccaggatc
      121 agcgtgccga agcagccggc aatgaacgag ccgatgcccg ccgccgccag cgccggcccg
      181 gcgcggccct tgcgcgccat ctggtagccg tcgatcaccg tcaccaccga cgaagattcg
      241 ccgggcaggt tgaccaaaat ggcggtggtc gagccgccgt actgcgcgcc gtagtagata
      301 ccggccagca tgatcagcgc ggccaccggc ggcagcgcgt aggtcgccgg cagcagcatg
      361 gcgatggtgg ccaccggacc gacgccgggc agcacgccga tcagcgtgcc cagaaagcat
      421 ccgatgaagg cgtacatcag gttctgcatc gtgaaggcga cgctgaaccc cagggacagg
      481 ttgttgatga gatccatgat tcagttcccc cttcagccgg tgatgaaggt cggccagacc
      541 tggaactgaa gattcaggcc ccagacaaac gcaaaccagc tgagcgccgc cagcaggatg
      601 gcgaggatca gcacttcgat gaacttgaac tcagtacccg ccgccgcagc gacgaaggtg
      661 agcacgaaga tcgcgatgat caaacccatg gccggaatgc cgagcaaagg ccagccgccc
      721 aacaggatgc cgaacaggaa gttggccgcg atgatgaaca ccagcggctt ccacgcccat
      781 ttgccgatcg gggcgccatc cggtgtttcg accaccatgg cctgaaacat cacgatgccg
      841 ccgatgatgg ccagcacgat gcccagcaac agcggaaagt agcccggccc catgcgggcg
      901 ccatcaccaa tgttgtagtt ggtcgcgccc catgcaaagg cgacgcctat ggcgaagtac
      961 agcaggccgg cgaagaaatc cttttgactc ttgatgcgca cgagtcatct cctcgaagat
     1021 ggaaaatacc agtgcgcatt gtcaccatcg gaaacgccgt gttcgatgtg gatttcacct
     1081 atcgcgcccc gggattaccc caatggccga gcgacttcat gcgttgaccg ccattgcagg
     1141 ttgctacgtt taccgtagct gcccgcgctt atccatcaag cgctggcggc ctgattgact
     1201 cgaaagtaat caatcagctc tacgagagcg gcggcgtggc ggcaatcact tcgggcagcg
     1261 tggtcagccc ttcggcagca cgcatggcgc cggccaggcg cagcggccgc atgccgtcgg
     1321 ccaccgcctg tttgcgcagc ggcgcggggc ccacgtcgtg ctgcaccagg cccttgaacg
     1381 cctcggtcac cgacagcagt tcatagatgc ccatgcggcc gcgaaagccc gtcatgcggc
     1441 aatccacgca gccgaccggc ttgtagggcc gccaattgcc gctgttgatc ttccacggct
     1501 tgacggcctc ggccagcaac tcggtggatg cgccatcgtc cggctgcttg cactgcgggc
     1561 acagcgtgcg caccaggcgc tgcgccagca cgccgagtat ggtggcgttg agcaaatagg
     1621 ccggcacgcc cagttccatc aggcgcgtga gcgcactggg cgcgtcgttg gtgtgcaggg
     1681 tgctgaacac caaatggccg gtgagcgcgg cctgcaccgc catctcggcg gtttccagat
     1741 cgcggatctc gccaatcatg atgatgtccg ggtcttgccg catcagcgcg cgcacgccct
     1801 cggcaaagct gaagtccagc tgcggctgca cctgcgtctg gttcagcgcc gggtcgatct
     1861 tctcgatcgg gtcttcgatg gtgctgacgt tgacctcttc cgtagccacg cggcgcagcg
     1921 tggaatacag cgtggtggtt ttgccggaac ccgtggggcc ggtcaccaga atgatgccgt
     1981 gcgggcgcga caccagttgc tcccagcgcc cggcgtcgtg tggggtgaag ccgagcgcgc
     2041 tcaagtcctt gaccgccgtg tcggggtcaa agatgcgcat caccagcttt tcaccaaacg
     2101 cggtgggcag cgtggacagc cgcatctcga tctcgtcgcc gcgcgggttg cgggtcttga
     2161 tgcggccgtc ctgcgggcgg cgcttttcca ccacgtccat gcggcccagc agcttgacgc
     2221 gcgcggtgat ggcgttcatc acgcccggcg gcacctgata gaccgggtgc agcacgccgt
     2281 cgatgcgaaa gcggatcacg ccctggtcgc gccgcggctc caggtgaatg tcgctggcgc
     2341 gctggtcgaa ggcgtactgc cacagccagt cgaccacctg gatcacgccc tggtcgttgg
     2401 cgtcgagctg
//
LOCUS       DAOMVO010000358         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13009_length_2410_cov_13.740552, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000358 DAOMVO010000000
VERSION     DAOMVO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13009_length_2410_cov_13.740552"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..880)
                     /gene="accD"
                     /locus_tag="PKV30_12755"
     CDS             complement(5..880)
                     /gene="accD"
                     /locus_tag="PKV30_12755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRL31119.1"
                     /translation="MSWLEKLLPPKIQKTEDGAERRQVPEGLWIKCPECETVLYKTDL
                     EGNQNVCPHCGHHHRIGARERLNVFLDHEGRYEIGQEVVPVDALKFKDSRKYPERIKE
                     AMENTGETDALVVMGGAVHSVSVVAACFEFEFMGGSMGSVVGERFVRGVETAIEQKVP
                     FICFTATGGARMQEGLLSLMQMAKTNAALTRLAKKGLPYISVLTDPTMGGVSAGFAFV
                     GDVVIAEPKALIGFAGPRVIENTVRVKLPEGFQRAEFLQQKGAVDFICDRRELREKIA
                     KVLAMLQRQPADAVQ"
     gene            complement(908..1717)
                     /gene="trpA"
                     /locus_tag="PKV30_12760"
     CDS             complement(908..1717)
                     /gene="trpA"
                     /locus_tag="PKV30_12760"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRL31120.1"
                     /translation="MSRIQSTFAKLQKEERTALIPYVTAGFPYADITPALMHGMVTAG
                     ADVIELGVPFSDPSADGPVIQKAGDRALALGVGLAQVLAMVKSFREKDQKTPVVLMGY
                     ANPVERYEQRHGDDAFIRDAAAAGVDGLLIVDYPPEECADFAAQLGAAGMDLIFLLAP
                     TSTEARMKQVAEVASGYVYYVSLKGVTGSGALNTAEVEAALPRIRQHVHVPVGVGFGI
                     RDAETAKAIGQVADAVVIGSRLIQVIEPQPHERVVAAAMDFLRPIRAALDS"
     gene            complement(1714..>2410)
                     /locus_tag="PKV30_12765"
     CDS             complement(1714..>2410)
                     /locus_tag="PKV30_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRL31121.1"
                     /translation="AGPHPYPMMVRDFQSVIGEECLTQMPEMLAAQGIAAQQPDAVIA
                     CVGGGSNAMGIFHPYIPFENTRLIGVEAAGEGLDSGKHSASLQRGSSGVLHGNRTYIL
                     QDVNGQITETHSISAGLDYPGVGPEHAWLKDIGRAEYVGATDAEALAAFHHLCRTEGI
                     IPALESSHAFAYAMKLAPTMRPDQSILVNLSGRGDKDIGTVADLDGAEVFDRPSMQGH
                     AVKGAARLAGGKA"
BASE COUNT          375 a          793 c          797 g          445 t
ORIGIN      
        1 ggggttattg gacggcgtct gccggctgcc gctgcagcat cgccagcacc ttggcgatct
       61 tctcgcgcag ctcgcgccga tcgcagatga aatcgaccgc gcccttttgc tgcaaaaact
      121 cggcgcgctg aaagccctcg ggcagcttga cgcgcacggt gttttcgatc acacgcggcc
      181 ccgcaaagcc gatcagcgcc ttcggctcgg caatcaccac atcgcccacg aaggcaaagc
      241 ccgccgaaac gccgcccatg gtcgggtcgg tcagcacact gatgtagggc aggcccttct
      301 tggccaggcg ggtgagggcg gcgttggtct tggccatctg catgagcgac agcaagccct
      361 cttgcatgcg cgcgccgccg gtggcggtaa agcagatgaa gggcaccttt tgctcgatgg
      421 cggtctcgac gccgcgcaca aagcgctcgc cgaccacgct gcccatgctg ccgcccatga
      481 actcgaactc gaagcaggct gcgaccacgc tgaccgagtg cacggcgccg cccatcacca
      541 ccagcgcgtc ggtctcgccg gtgttttcca tcgcttcctt gatgcgctcg ggatacttgc
      601 ggctgtcctt gaatttcagc gcatcgaccg gcaccacttc ctgcccgatc tcgtagcggc
      661 cctcgtggtc gagaaacacg ttgagccgct cgcgcgcgcc gatgcggtgg tggtgaccgc
      721 agtgcgggca gacgttctgg ttgccctcca ggtcggtctt gtagagcacc gtttcgcact
      781 cggggcattt gatccacaag ccttcgggca cctggcggcg ctcggcgccg tcttcggttt
      841 tctggatctt tgggggcagc agtttttcaa gccagctcat ggcagaactc cttgtttgat
      901 agcgcgatta tgaatccagc gccgcgcgga tcgggcgcag gaagtccatc gcagcggcca
      961 ccacacgctc gtgaggctgc ggttcaatga cctggatcag ccggctgccg atgaccaccg
     1021 catcggccac ctggccgatg gctttggcgg tttcggcatc gcggataccg aagccgacgc
     1081 cgaccggcac atgcacgtgc tggcggatgc ggggcagggc ggcttcgacc tcggccgtgt
     1141 tgagcgcgcc cgagccggtc acgcccttga gcgacacgta gtacacgtag ccgctggcga
     1201 cctcggccac ctgcttcatg cgcgcctcgg tgctggtggg agccagcaga aagatgaggt
     1261 ccatgcccgc agcccccagc tgcgcggcga agtcggcgca ttcctcgggc gggtagtcga
     1321 cgatcagcag gccatcgacg cccgcggcgg cggcgtcacg gatgaaggca tcgtcgccat
     1381 ggcgttgttc atagcgttcc accgggttgg cgtagcccat cagcaccacg ggcgttttct
     1441 ggtctttctc gcgaaagctt ttcaccatcg ccagcacctg cgccaggccg acgcccagcg
     1501 ccagcgcgcg gtccccggct ttctggatca cggggccgtc ggcactgggg tcggaaaacg
     1561 gcacgcccag ctcgatcacg tcggcgccgg cggtgaccat gccgtgcatc agcgcgggcg
     1621 taatgtcggc atacggaaag cccgccgtca catacgggat cagcgccgtg cgctcttctt
     1681 tctgcagctt ggcaaacgtg gactggatgc ggctcatgcc ttgcccccgg ccaaccgggc
     1741 cgcgcccttg acggcatggc cttgcatcga tgggcggtcg aacacttcgg cgccatcgag
     1801 atcggccacg gtgccgatgt ccttgtcgcc gcgtcctgac aggttgacca ggatggattg
     1861 atcgggccgc atcgttggtg cgagcttcat ggcgtaggca aaggcgtggc ttgattccag
     1921 cgcggggatg atgccctcgg tacggcacag gtggtggaag gcggccagcg cctcggcatc
     1981 cgtcgcgccg acgtattcgg cgcggccaat gtccttcagc caggcgtgct cggggccgac
     2041 gccggggtag tcgaggccgg ccgagatgct gtgcgtctcg gtgatctggc cgttgacgtc
     2101 ttgcaggatg taggtgcggt taccgtgcag cacgccgctg gagccgcgct gcaggctggc
     2161 cgagtgtttg ccgctgtcca ggccttcgcc ggccgcttcg acaccgatca ggcgcgtatt
     2221 ttcaaacggg atgtaggggt gaaagatgcc catggcgttg ctgccgccgc ccacgcaggc
     2281 gatcacggca tcaggctgct gcgcggcgat gccctgcgct gccagcatct cgggcatctg
     2341 cgtgaggcat tcctcgccga tcacgctctg gaagtcgcgc accatcatcg ggtaggggtg
     2401 cgggccggcc
//
LOCUS       DAOMVO010000359         2384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13191_length_2384_cov_8.901675, whole genome shotgun sequence.
ACCESSION   DAOMVO010000359 DAOMVO010000000
VERSION     DAOMVO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13191_length_2384_cov_8.901675"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKV30_12770"
     CDS             complement(<1..628)
                     /locus_tag="PKV30_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL31122.1"
                     /translation="MTTLEDCRARDATDPLAPLKDLFDLPDGVMYLDGNSLGALPRAV
                     PARMAQVVAQEWGRDLIQSWNKSGWLDLPRRVGDKIAPLIGAGPGEVVAADTTSINLY
                     KVLSAALNLAAQDAPGRRTIVSERSNFPTDLYIAEALCQERGCTLKLIEPHEIAASLG
                     ADVAVLMLTHVNYRTGAMHDMSAITQAAHAAGALVVWDLAHSAGAVPVD"
     gene            complement(696..1328)
                     /gene="kynB"
                     /locus_tag="PKV30_12775"
     CDS             complement(696..1328)
                     /gene="kynB"
                     /locus_tag="PKV30_12775"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HRL31123.1"
                     /translation="MSQPQLWDISPPVHAGAPVFPGDTAYAQQWSAQIGPGCPVNVSA
                     LTLSPHVGAHADAPLHYDSAGAAIGAVDLAPYLGPCRVIHAIGVRPLILWEHLAHATQ
                     ALPPRVLVRTYERMPVDAWDAQLAAFAPETVERLADAGVRLIGIDTASIDPADSKTLD
                     SHQVIRRRDVRVLENLVLDEVPEGDYELIALPLKLVTADASPVRAVLRAL"
     gene            complement(1578..1904)
                     /locus_tag="PKV30_12780"
     CDS             complement(1578..1904)
                     /locus_tag="PKV30_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRL31124.1"
                     /translation="MAGERPDCQHCGACCASFRVDFAVDELLSSGGSVPDGLTVEVTA
                     HTCRMRGTDHVPARCAALTGRVGERVACGIYEWRPAPCREFSAGGDACLRARARLGLT
                     PLQAAE"
     gene            2135..>2384
                     /locus_tag="PKV30_12785"
     CDS             2135..>2384
                     /locus_tag="PKV30_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRL31125.1"
                     /translation="MRTDMFQQVLEELKGSSADVEASALISTDGLMIASALPQGMDED
                     RVGAMSAALLSLGDRAAKELARGTLERVLLQGERGYVIM"
BASE COUNT          394 a          774 c          824 g          392 t
ORIGIN      
        1 aatcgacggg caccgcgccc gcgctgtgcg ccaggtccca caccaccagc gcgcccgcgg
       61 cgtgcgcagc ttgggtgatg gcggacatgt cgtgcatggc gccagtgcgg tagttgacgt
      121 gggtgagcat cagcacggcc acgtctgcgc ccaagctggc cgcgatttcg tgcggctcga
      181 tcagcttgag cgtgcaaccg cgctcctggc acagcgcctc ggcgatgtac aggtcggtgg
      241 ggaagttgct gcgttcgctg acgatggtgc ggcggccggg cgcgtcttgc gcggccaggt
      301 taagcgcggc ggacagcact ttgtagaggt tgatcgaggt cgtgtcggcc gccaccacct
      361 cgccggggcc ggcgccgatg agcggcgcga tcttgtcgcc cacgcggcgc ggcaggtcga
      421 gccagccgga cttgttccag ctctggatca aatcgcgccc ccactcttgc gcgacgactt
      481 gcgccatgcg cgcgggcacg gcgcggggca gggcgccgag cgagttgccg tcgaggtaca
      541 tcacgccgtc aggcagatcg aacaggtctt tgagcggcgc cagcggatcg gtggcgtcgc
      601 gggcgcggca gtcttcgagt gtggtcatgg gtcggtttga tataaaaaca ggagctgctt
      661 gcgcttgctg gacaagcgct ggcggccgat ttgtctcata aagcgcgcag caccgcccgc
      721 accggcgacg catccgccgt gaccagcttc aagggcaggg cgatcagctc gtaatcgcct
      781 tcgggtacct cgtcgagcac caggttttcc aacactcgca catcgcgccg gcggatgacc
      841 tggtggctgt ccaaggtctt gctgtcggcc gggtcgatgc tcgcggtgtc gatgccgatc
      901 aggcgcacgc cggcgtcggc caggcgctcc acggtctcgg gcgcgaaggc ggcgagctgc
      961 gcgtcccacg catcgaccgg catgcgctca taggttctga ccagcacacg cggcggcagc
     1021 gcttgtgtgg cgtgcgccag gtgctcccaa agaatgagcg gccgcacgcc aatggcgtga
     1081 atcacgcggc acgggcccag atacggcgcc aagtccaccg cgccaatcgc agcgcccgcc
     1141 gagtcgtaat gcagcggcgc atcggcgtgc gcgccgacgt gtggcgacag cgtcagcgcg
     1201 ctcacgttga ccgggcagcc ggggccgatc tgcgcgctcc attgctgcgc atacgcggtg
     1261 tcgcccggaa agaccggcgc accggcatgc accggcggcg agatgtccca aagctgcggc
     1321 tggctcatgc ggccacctta gcgctttgcg cgcggcgcag tgtcggttat ttccaactcg
     1381 cgccggctct gcggccgcgg ccgccggcgc ttggcgcaca gggcccgcaa cacggccggg
     1441 cagggtgaac gcgggtggct gccatgactg cgggggctgc ggtgcatgtg ctggcgacac
     1501 accgcctttt caacaaaaaa ggccgccagc gcttttacag aaagcgcgag cagctaccat
     1561 aaagatagca atcgcgatca ttccgccgcc tgcagcggcg tcagcccaag gcgcgctcgc
     1621 gcacgtaggc aggcgtcgcc gccggcgctg aactcccggc agggagcagg ccgccactcg
     1681 tagatgccgc aggccacgcg ttcgcccacg cgcccggtga gcgcggcgca ccgcgccgga
     1741 acatggtcgg tcccgcgcat gcgacaggta tgggcggtca cctccacggt cagcccgtcg
     1801 ggaacgctgc cgccgcttga cagcaattcg tctacggcga aatcaacccg gaaactggcg
     1861 cagcacgctc cgcagtgctg gcagtccggt cgctcgcccg ccatcgattt catagctgcg
     1921 cgcgctgatg gatgcttaag ttcagtatcg tcatacgtcg acactcgggt attgcggcga
     1981 gcgtgaaatc cgtccgcgca ttagtgcacg attgtcatgt catgtgaggt gcagatgctg
     2041 gaaaacgcct cttgtttccg ctctaatcag ggctgagccg gttcaccgaa gtcgatggcc
     2101 cggtgcaggt caatcactca aagaggatac gcaaatgcgc acagacatgt tccagcaggt
     2161 gctggaagaa ttgaaaggtt cgtccgccga cgtcgaggcg tcggcactga tttccaccga
     2221 cggtctgatg atcgcctcgg ccctgccgca gggcatggac gaggaccgtg tgggcgccat
     2281 gtcggcggcg ctgctgtcgc tgggcgatcg cgccgccaag gaactggcgc gcggcacgct
     2341 ggagcgcgtg ctgctgcagg gcgagcgcgg ctacgtgatc atga
//
LOCUS       DAOMVO010000360         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13254_length_2373_cov_13.944780, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000360 DAOMVO010000000
VERSION     DAOMVO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13254_length_2373_cov_13.944780"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PKV30_12790"
     CDS             <1..1350
                     /locus_tag="PKV30_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HRL31126.1"
                     /translation="VHERLLRVLGAARWPARYVMQHPGVIDELASPSLLDDRFDAAAF
                     EQELEWRHQALAATGEDDEENLLNLLRRGHHAEVFRTLVRDVEGRISVEQVADDLSAL
                     ADCILRVTMRWCWPRLRQKHREEPRFGVIGYGKLGGKELGYGSDLDVVFVFDDEHENA
                     QEIYSAFVRKLINWLTIKTGEGDLFEIDTALRPNGGSGLLVTRFEAYADYQENRGSNT
                     AWTWEHQAMTRARMVPPRSPAAHGARALQAAEPPESVPLRAVQARAGEAGAAALSPEG
                     AAPGLGSGPAAGRMVPPSIASPDAPPDNLVARFDAVRHAVITAPRDQAALRGEIVAMR
                     EKMRAAHPVKQGLFDVKHSPGGMVDAEFVTQYLVLAHSGDHPGLQPNLGNISLLRRAE
                     EAGLLPEGVGQRAADAYRALRRVQHRARLDEQPTQVAPDTLAGERAAIQRLWQVVFE"
     gene            1471..2082
                     /locus_tag="PKV30_12795"
     CDS             1471..2082
                     /locus_tag="PKV30_12795"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRL31127.1"
                     /translation="MSPRAWPLACLLMIAGSALVWLLAANDAVPPDWLIWRFDGWRQQ
                     PWTLWTGPLLHLVLPHAIANALALAALAVLGAALAVPPRDALALLLAWPLGTLALLWV
                     PGVGASYGLGGAVHAAAAILAVRTLQNPDTRVLGLLLAGGLLIKLRLERAWAVPIGFD
                     SGWAFNVIYAAHLTGAVAGAALALLLEACHRLFGRRPPRAAVD"
     gene            complement(2143..>2373)
                     /locus_tag="PKV30_12800"
     CDS             complement(2143..>2373)
                     /locus_tag="PKV30_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRL31128.1"
                     /translation="VLRVTAPREPCGKFAAVMGFAEAGRVMVREARCGFYLAVDAPGP
                     LQAGAALRVVPGRRGLSVADAVRAKWARHRNI"
BASE COUNT          326 a          863 c          837 g          347 t
ORIGIN      
        1 gtgcacgagc gcctgctgcg cgtgctgggc gcggcgcgct ggccggcgcg ctacgtgatg
       61 cagcacccgg gcgtcatcga cgagctggcc agccccagcc tgctggacga ccgcttcgat
      121 gccgccgcct tcgagcaaga gctggaatgg cgccaccagg cgctggccgc caccggcgag
      181 gacgacgagg aaaacctgct caacctgctg cgccgcggcc accacgccga ggtgtttcgc
      241 accctggtgc gcgacgtcga gggccgcatc agcgtcgagc aggtggccga cgatttgtcg
      301 gcgctggccg actgcatcct gcgcgtcacc atgcgctggt gctggccgcg cctgcgccag
      361 aagcaccgcg aagaaccgcg cttcggcgtc atcggctacg gcaagctggg cggcaaggaa
      421 ctgggctacg gcagcgacct cgacgtcgtt ttcgtcttcg acgacgagca cgagaacgcg
      481 caggaaatct attccgcctt cgtgcgcaag ctgatcaact ggctgaccat caagaccggc
      541 gagggcgatc tgttcgagat cgacaccgcg ctgcgcccca acggcggctc gggcctgctc
      601 gtgacccgct tcgaggccta cgccgactat caggaaaacc gcggcagcaa taccgcgtgg
      661 acgtgggagc accaggccat gacgagggca aggatggtgc ccccacgctc cccggctgcg
      721 catggtgcgc gggccttgca ggccgcggag ccgccggagt cggttcctct tcgggctgtc
      781 caggctcgcg caggcgaggc tggagccgca gccctcagcc ccgagggggc cgcgcccggc
      841 cttgggagcg gcccggcggc cgggcggatg gtgccgccgt ccatcgcatc accggacgcg
      901 ccgcccgaca acctcgtcgc ccgcttcgac gccgtgcgcc acgccgtcat caccgcgccg
      961 cgcgaccagg cggcgctgcg cggcgagatc gtcgccatgc gcgagaagat gcgcgcggcg
     1021 cacccggtca agcaggggtt gttcgacgtc aagcacagcc cgggcggcat ggtggatgcc
     1081 gagttcgtca cccaatacct ggtgctggcg cacagcggcg accacccggg gctgcagccg
     1141 aacctgggca acatcagcct gctgcggcgc gctgaagaag ccggcctgct gcccgaaggt
     1201 gtgggccagc gggcggccga tgcctaccgc gcgctgcggc gcgtgcagca ccgcgcgcgg
     1261 cttgacgagc aaccgacgca ggtggcgccc gacacgctgg ctggcgaacg cgccgccatc
     1321 cagcggctgt ggcaagtggt ttttgaatga aaatgcgggc tggcgcttgt ccgatcagcg
     1381 ccagcagcta gcaaattcat accaaccccg cgccgtgcgc gccccggttt gccgggtgcc
     1441 gcggcgcccg tcgcccgttt cttgcaccgc atgtcgcccc gcgcctggcc ccttgcctgc
     1501 ctgctgatga tcgccggcag cgcgttggtg tggttgctgg ccgccaacga cgcggtgccc
     1561 ccagactggc tgatctggcg ctttgacggc tggcgccagc agccgtggac gctgtggacc
     1621 ggcccgctgc tgcacctggt gctgccgcac gcgatcgcca acgcgctggc gctggcggcg
     1681 ctggccgtgc tgggcgcggc gctggccgtg cccccacgcg atgcgctggc gctgctgctg
     1741 gcctggccgc tcggcacgct ggcgctgctg tgggtgcccg gcgtgggcgc gagctacggc
     1801 ctgggcggcg cggtgcacgc ggccgcggcg atcctggcgg tgcgcacgct gcaaaacccc
     1861 gacacccgcg tgctgggcct gctgctggcc ggcggcctgc tcatcaagct gcggctggag
     1921 cgcgcgtggg cggtgccgat cggctttgac agcggatggg ccttcaacgt catctacgcc
     1981 gcccacctga ccggggccgt ggcgggtgcg gcactggcgc tgttgctgga ggcttgtcac
     2041 cgcctgttcg gccgccgccc gccacgcgct gcggtcgatt gattttctat tgaattgata
     2101 gctgcttgcg cttgccagac aagcgctggc ggcctttttt attcagatgt tccggtgtcg
     2161 cgcccacttg gcgcgcaccg cgtcggccac cgacaggccg cgccgccccg gcaccacgcg
     2221 cagcgcggca cccgcctgca gcgggccggg cgcatccacc gccaggtaaa agccgcagcg
     2281 cgcctcgcgc accatcacgc gcccggcctc ggcaaagccc atcaccgccg cgaacttgcc
     2341 gcacggctcg cgcggcgcgg tcacgcgcag cac
//
LOCUS       DAOMVO010000361         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13317_length_2365_cov_14.187879, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000361 DAOMVO010000000
VERSION     DAOMVO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13317_length_2365_cov_14.187879"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2365)
                     /locus_tag="PKV30_12805"
     CDS             complement(<1..>2365)
                     /locus_tag="PKV30_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL31129.1"
                     /translation="TIHTEAGVVTQRVADAAEAAGLVFACDPTSAEASCIGGNIAMNA
                     GGKKAVLWGTALDNLASWRMVTPDAGWLEVTRLNHNLGKIHDAEVASFELKYFDASGK
                     KLVKTEQLDIPGATFRKEGLGKDVTDKFLAGLPGVQKEGCDGLITSARWVLHRMPEHT
                     RTVCLEFFGHAKDAVPSIVEIKDFMFAEAKRTGVLLAGLEHLDDRYLRAVGYSTKSKR
                     GGLPKMVLIGDIAGDDADAVARATSEVVRIANSRSGEGFVAVNADARKKFWADRKKTA
                     AISRHTNAFKINEDVVIPLPRMAEYTDGIERINIELSLRNKIELADELEAFLERGNLP
                     LGQVPDGSVLGSPELLAEKVAQALAVVREVRALWQGWLRDVDTLFPQLQDHSLRASWK
                     TQIRAPLQDIFTGSDFEPLMKALGDVQQRVLKGRVWIALHMHAGDGNVHTNIPVHSDN
                     YAMLQTAHEAVDRVMALARRLDGVISGEHGIGITKLEYLSDDEIAPFAAYKQRVDPQG
                     HFNRGKLLRNKELSAQSGQALAADLTNAYTPSFGLMGHESIIMQQSDIGAIADSIKDC
                     LRCGKCKPDCSTHVPRANLLYSPRNKILATSLLVEAFLYEEQTRRGVSLKHWEEFEDV
                     ADHCTVCHKCLSPCPVKIDFGDVTMNMRNLLRKMGKKSVRPGNAAAMFFLNATDPRVI
                     NTTRAAMTGVAFKAQRAAVDFFKVAGRHQTKHPPATVGKPPLREEIIHFVNKKLPAGL
                     PSKTARALLDIEDRAYVPVIRDPKQTTADTEAVFYFPGCGSERLFSQV"
BASE COUNT          350 a          777 c          778 g          460 t
ORIGIN      
        1 acctggctga acagccgctc cgacccacag ccgggaaagt agaacaccgc ctcggtatcg
       61 gccgtggtct gcttcgggtc gcggatcacc ggcacgtagg cgcggtcttc gatgtcgagc
      121 agcgcgcgcg ccgtcttgct gggcaggccc gcgggcagct tcttgttgac gaagtggatg
      181 atctcctcgc gcagcggcgg cttgccgacg gtggcgggcg ggtgcttggt ctggtggcga
      241 ccggcgacct tgaagaaatc caccgccgcg cgctgcgcct tgaaggccac gccggtcatc
      301 gccgcgcgcg tggtgttgat gacgcgcgga tcggtcgcgt tcaaaaagaa catcgccgcc
      361 gcgttgccag gccgcacgct tttcttgccc atcttgcgca gcaggttgcg catgttcatg
      421 gtcacgtcgc caaagtcgat cttcaccggg cacggcgaca ggcacttgtg gcacaccgtg
      481 cagtggtcgg ccacgtcctc gaattcttcc cagtgcttga ggctgacgcc gcggcgcgtc
      541 tgctcttcgt acaggaaagc ctcgaccagc agcgaggtcg ccaggatctt gttgcgcggg
      601 ctgtacagca ggttggcgcg cggcacgtgc gtgctgcagt cgggcttgca cttgccgcag
      661 cgcaggcagt ccttgatcga atcggcaatg gcgccgatgt cgctctgctg catgatgatc
      721 gactcgtgcc ccatcagccc gaagctgggc gtgtaggcat tggtcaaatc ggccgccagc
      781 gcttgtccag actgcgcgga cagctcttta tttcggagca acttaccgcg attgaagtgg
      841 ccttgcggat cgacgcgctg cttgtaggcg gcaaagggcg cgatctcgtc gtcgctcagg
      901 tattcgagct tggtgatgcc gatgccgtgc tcgccgctga tcacgccatc cagccggcgc
      961 gccagcgcca tcacgcgatc caccgcctcg tgcgcggttt gcagcatggc gtagttgtcg
     1021 ctgtgcacgg ggatgttggt gtgcacgttg ccgtcgcccg cgtgcatgtg cagggcgatc
     1081 cacacacggc ccttcagcac gcgctgctgc acgtcgccca gcgccttcat cagcggctcg
     1141 aaatcgctgc cagtgaagat gtcttgcagc ggcgcgcgga tctgcgtctt ccagctggcg
     1201 cgcaggctgt ggtcttgcag ttgcgggaac agcgtgtcga cgtcgcgcag ccaaccttgc
     1261 cacagcgcac gcacctcgcg caccacggcc agcgcctgcg ccaccttctc ggccagcagt
     1321 tcgggcgagc cgagcaccga gccgtcgggc acctgcccga gcggcaggtt gccgcgttca
     1381 agaaaggcct ccagttcgtc ggccagctcg atcttgttgc gcaagctgag ctcgatgttg
     1441 atgcgctcga tgccgtcggt gtattcggcc atgcgcggca gcggaatcac cacgtcttcg
     1501 ttgatcttga aggcgttggt gtggcggctg atggcggcgg tcttcttgcg gtcggcccag
     1561 aacttcttgc gcgcatcggc attgacggcg acaaagccct cgcccgagcg cgagttggcg
     1621 atgcgcacca cttcgctggt cgcgcgcgcc acggcgtcgg catcgtcgcc cgcgatgtcg
     1681 ccgatcagca ccatcttcgg caagccgccg cgcttggact tggttgagta gcccacggcg
     1741 cgcaggtagc ggtcgtccag gtgttccagc ccggcgagca gcacacccgt gcgcttggcc
     1801 tcggcaaaca tgaagtcctt gatctcgacg atgctgggca ccgcatcctt ggcgtggcca
     1861 aagaattcga ggcacacggt gcgcgtgtgc tcgggcatgc ggtgcagcac ccagcgcgcg
     1921 ctggtgatca ggccgtcgca gccttccttc tgcacgcccg gcaggccggc caggaacttg
     1981 tcggtcacgt ccttgcccag cccctccttg cggaaggtgg cgccggggat gtcgagttgt
     2041 tccgtcttga ccagcttctt gccgctggcg tcgaaatact tcaactcgaa gctggccacc
     2101 tcggcatcgt ggatcttgcc caggttgtgg ttgaggcgcg tgacttcgag ccaacccgcg
     2161 tccggcgtca ccatgcgcca gctggccagg ttgtccagcg ccgtgcccca cagcacggcc
     2221 ttcttgccgc cggcgttcat ggcgatgttg ccaccgatgc agctggcttc ggcgctggtc
     2281 gggtcgcagg caaacaccag gcccgcagct tcggccgcat cggccacgcg ctgcgtcacc
     2341 acgccggctt cagtgtggat ggtgg
//
LOCUS       DAOMVO010000362         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13365_length_2357_cov_18.279322, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000362 DAOMVO010000000
VERSION     DAOMVO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13365_length_2357_cov_18.279322"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKV30_12810"
     CDS             <1..342
                     /locus_tag="PKV30_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905112.1"
                     /note="involved in the export of lipooligosaccharides
                     during nodulation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation factor ABC transporter ATP-binding
                     protein NodI"
                     /protein_id="HRL31130.1"
                     /translation="FMDEAERLCQRLLVLDHGRKIAEGRPRELIAEHLEPEVVEVYGQ
                     GALQLAESALAARAERVERSGETVFFYTRQARPLMQALEAHPHLRTLHRPANLEDLFL
                     KLTGRQIREDG"
     gene            506..1306
                     /locus_tag="PKV30_12815"
     CDS             506..1306
                     /locus_tag="PKV30_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL31131.1"
                     /translation="MNTPSVWSPPRLSMRWWPVFQRNLLVWRKLAIPSLLANIGEPLL
                     WLVAFGYGLGAMVGRVPTHGQEVPYILFLASGSICMSAMNAASFEALYSAFSRMHVQK
                     TWDGILNTPISLDDVVLAEMLWAGFKALFSVIAILGVMLALKISLSPKLILVPPILLI
                     AGVTFASMALVFNAKAKGYDFFTYYFAVLLSPMMFLSGVFFPRTQLPAFAQAITDWLP
                     LTAAVELVRPLFLDQWPADAARHLAVLALYAIASFWIALAFTRQRFRR"
     gene            1478..2332
                     /locus_tag="PKV30_12820"
     CDS             1478..2332
                     /locus_tag="PKV30_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRL31132.1"
                     /translation="MSITTVGIVGAGTMGNGIAQACAVSGINVVMVDIAQPAVDKGLA
                     TVGKSLDRLLQKEKITEADKAAALGRIKGSTSYDDLKGAQLVVEAATENHELKNKILK
                     QLDDLLPPEVIIATNTSSISITQLAAVTKRADKFVGMHFFNPVPMMALVEIIRGLQTS
                     DATHDAVKELALKLGKSPITVKNAPGFVVNRILVPMINEAFFVLAEGLATPEDIDTGM
                     KLGCNHPIGPLALADMIGLDVCLAVMEVYLEQFGDSKYRPCPLLREYVAAGRLGRKTG
                     MGVYSYAK"
BASE COUNT          421 a          755 c          752 g          429 t
ORIGIN      
        1 ttcatggacg aggccgagcg cctgtgccag cgcctgctgg tgctcgacca cggccgcaag
       61 atcgccgagg gccgcccgcg cgagctgatt gccgagcacc tggagccgga ggtggtggag
      121 gtctatggcc agggcgcgct gcaactggcc gaatccgcgc tggccgcgcg cgccgagcgg
      181 gtggagcgaa gcggcgagac ggtgttcttc tacacccgcc aggcgcgccc gctgatgcag
      241 gcgctggagg cgcatcctca cctgcgcacg ctgcaccggc ccgccaatct ggaggatttg
      301 ttcctcaagc tcaccggccg acagattcgg gaggacgggt gatgggcaca aaacgtcaac
      361 agccggacgc gaaggacgca aaggattcgc gaagaacgcg aaagaaaaca cgtcttttgg
      421 atgttccttt tgcgtgcttc gcggaatctt cgcgtccttc gcgtctggtt gtttgtgcat
      481 ccattcgccg tcatcagagc tcgccatgaa cacgccttcc gtctggtcgc caccgcgcct
      541 gtccatgcgc tggtggcccg tgttccagcg caacctgctg gtgtggcgca agctggcgat
      601 tcccagcctg ctggccaaca tcggcgagcc gctcttgtgg ctggtggcct tcggctacgg
      661 cctgggcgcg atggtgggcc gcgtgcccac gcacgggcaa gaggtgccct acatattgtt
      721 tttagccagc ggcagcatct gcatgagcgc catgaacgcg gccagcttcg aggcgctgta
      781 ttcggccttc tcgcgcatgc acgtgcaaaa gacctgggac ggcatcctga acacgcccat
      841 cagcctggac gacgtggtgc tggccgagat gttgtgggct ggcttcaagg cgctgttctc
      901 ggtcatcgcc atcctgggcg tgatgctggc gctgaagatc agcctgagtc cgaagctgat
      961 cctggtgccg ccgatcctgc tgatcgctgg cgtcaccttt gccagcatgg cactggtgtt
     1021 caacgccaag gccaagggct acgacttctt cacctactac ttcgcggtgc tgctgtcgcc
     1081 gatgatgttc ttgagcggcg tgttctttcc gcgcacgcag ctgccggcgt ttgcgcaagc
     1141 catcaccgac tggctgccgc tgacagcagc ggtcgaactg gtgcggccgc tgttccttga
     1201 ccagtggccg gccgatgcgg cgcgccatct ggccgtgctg gcgctgtacg ccattgccag
     1261 cttctggatc gcgctggcct tcacgcgaca gcgctttcgg cgctgaagct catgcattaa
     1321 aacaggcgcc agcgcttgat tgacaagcgc tggcagctat gttttatgta gcaattcgat
     1381 cgtttggcaa actgacgcgg cgtgccgcta acatcgtcag cttgcgtccc gcacaggccg
     1441 gggcctgcct tcattcatca cacgaggaaa aaccatcatg agcatcacca ccgtgggcat
     1501 cgtcggtgcc ggcaccatgg gcaacggcat cgcgcaggct tgcgccgtgt cgggcatcaa
     1561 cgtcgtcatg gtcgacatcg cccagccggc ggtcgacaag gggctggcca ccgtcggcaa
     1621 gagcctggac cgcctgctgc aaaaagaaaa gatcaccgaa gccgacaagg cggccgcgct
     1681 gggccgcatc aagggcagca ccagctacga cgacctgaag ggcgcgcaac tggtggtcga
     1741 ggccgcgacc gagaaccacg aactgaagaa caagatcctc aagcagctcg acgacctgct
     1801 gccgcccgag gtgatcatcg ccaccaacac ctcgtcgatt tccatcacgc aactggccgc
     1861 cgtcaccaag cgcgccgaca agttcgtcgg catgcacttc ttcaatccgg tgccgatgat
     1921 ggcgctggtc gagatcatcc gcggcctgca gacttcggac gccacgcacg acgcggtcaa
     1981 ggaactggcg ctgaagttgg gcaagtcgcc catcacggtg aagaacgcgc caggcttcgt
     2041 ggtcaaccgc atcctcgtgc cgatgatcaa cgaggccttc ttcgtgctgg ctgagggctt
     2101 ggccacaccg gaagacatcg acaccggcat gaagctgggc tgcaaccacc ccatcggccc
     2161 gctggcgctg gccgacatga tcggcttgga cgtgtgcctg gcggtgatgg aggtgtatct
     2221 ggagcagttc ggcgacagca agtaccgccc ctgcccgctg ctgcgcgaat acgtcgccgc
     2281 cggccgcctt ggccgcaaga ccggcatggg cgtgtacagc tacgccaagt gagggcggcg
     2341 ggcgcgcggc atactgg
//
LOCUS       DAOMVO010000363         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13443_length_2347_cov_10.444154, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000363 DAOMVO010000000
VERSION     DAOMVO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13443_length_2347_cov_10.444154"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="PKV30_12825"
     CDS             <1..1554
                     /locus_tag="PKV30_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31133.1"
                     /translation="DLDFARKLLSTPEIVSAFGVDAKYTDARLAGQLHSWASFGPPTG
                     ALWPPTAKEARDHAVIRPLIQNPWTLLTVQAPSDTPTTQPADLPHPRQAWQSPKPSVY
                     TPPAPYWQGTLRPASDADIWLSTGFARFERLLADLHAPANAGSAPTDPQDDVGVELAY
                     YRSWFGRAARAVSGQPLAQTRADMGDPLGYQRVAGKGVLFLHTLRDLIGAPAFDAAMR
                     RFGRERDGQAVSARDLQAILQPHTRRPLGPLFDWWTQQRGLPMLAVTAAEARATGSGW
                     ETRVTLDTTRIGPALSVPVTVETAEGDVTQRMVFDADHPQMRIATRAKPLRVVVDKHG
                     TTARGNGSPFTILTMDDEPEHALIIYGTQDDEVGNLEAARLLQTALRRREHNVQPPIK
                     PDREVSDDDLRGHHLLLVGRPSTNAVSQRFAAQWGVDFGARSFTVRGQRYTHPESAVL
                     AAGDNPLDARWSVVLVAGLSSLGTYQVVGRFADDLLTYAPLVIAPFGRDMRDVVPPLP
                     ELTVVPVIR"
     gene            complement(1636..1962)
                     /locus_tag="PKV30_12830"
     CDS             complement(1636..1962)
                     /locus_tag="PKV30_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31134.1"
                     /translation="MTRSPDRMWLREELLQLLSVAGTLAGLSITAVALLHTVGHADAT
                     PTIVDDMLVISALLFLLCTYAIFFALRTRRDTVARRLERLADVLFLLALTGMVSSGFV
                     MFYTLL"
BASE COUNT          378 a          835 c          804 g          330 t
ORIGIN      
        1 gacctcgact tcgcgcgcaa gctgctctcg acgcccgaaa tcgtctcggc cttcggcgtc
       61 gacgccaagt acaccgacgc gcggctggcc ggccagctgc acagctgggc ctcgtttggc
      121 ccgcccaccg gcgcgctgtg gccgccgacc gccaaggagg cgcgcgatca cgcggtgatc
      181 cgcccgctga tccagaaccc gtggacgctg ctcaccgtgc aggcgccgtc cgatacgccg
      241 acgacccagc cggccgatct gccgcatccc cgccaggcct ggcaatcgcc caagcccagc
      301 gtgtatacgc cgcccgcgcc gtactggcag ggcacgctgc ggccggcgag cgatgccgac
      361 atctggctgt cgaccggctt cgcgcgtttt gaacgcctgc tcgccgacct gcacgcgccc
      421 gcgaatgccg gcagcgcgcc gaccgacccg caggacgatg tgggcgtcga actggcctac
      481 taccgcagct ggtttggccg cgccgcgcgt gccgtgagcg gccagccgct ggcccagacg
      541 cgcgccgaca tgggcgatcc gctcggctac cagcgcgtgg cgggcaaggg cgtgctgttc
      601 ctgcacacgc tgcgcgactt gatcggcgcg ccggccttcg atgccgccat gcgccgcttc
      661 gggcgcgagc gcgatgggca ggccgtgtcg gcgcgcgacc tgcaagccat cttgcagccg
      721 cacacgcgcc ggcccctcgg cccgctgttc gactggtgga cgcagcagcg gggcctgccg
      781 atgctggccg tgaccgccgc cgaggcacgc gccaccggca gcggctggga aacccgggtg
      841 acgctcgaca ccacgcgcat cggccccgcg ctgtcggtgc cggtgacggt ggaaacggcc
      901 gagggcgacg tcacccagcg catggtgttc gacgccgatc acccgcagat gcgcattgcc
      961 acgcgcgcaa agccgctgcg cgtggtggtc gacaagcacg gcaccaccgc gcgcggcaat
     1021 ggctcgccct tcaccatcct caccatggac gacgaaccgg agcacgcgct catcatctat
     1081 ggcacgcagg acgacgaagt gggcaacctg gaggcggcgc gcctgctgca gaccgcgctg
     1141 cgccggcgcg agcacaacgt gcagccgccc atcaagcccg accgcgaggt gagcgatgac
     1201 gatctgcgcg gccaccacct gctgctggtc ggccggccat cgaccaacgc cgtcagccaa
     1261 cgctttgccg cgcaatgggg tgtggacttc ggcgcgcgct cgttcaccgt gcgcggccag
     1321 cgctacaccc accccgaaag cgccgtgctg gccgccggcg acaacccgct cgacgcgcgc
     1381 tggtcggtgg tgctggtggc gggcttgagc agcctgggca cctatcaggt ggtgggccgg
     1441 tttgcggacg atttgctgac ctacgcgccc ctggtcatcg ccccgttcgg gcgtgacatg
     1501 cgcgacgtgg tgccgccgct gcctgagctg acggtggtgc cggtgattcg atagttcgag
     1561 aagcgttttt tttcaagcca aactggccgc tggcgcttga tgggcaagcg cgggcagcta
     1621 tcgaatcgag agtattcaga gcagggtgta gaacatcacg aagcccgagc tgaccatgcc
     1681 cgtcagggcc agcagaaaca gcacgtcggc cagccgctcg agccgccggg cgacggtgtc
     1741 gcggcgcgtg cgcagggcaa agaaaatggc gtaggtgcac agcagaaaca gcagtgccga
     1801 aatcaccagc atgtcgtcga cgatggtggg cgttgcgtcc gcatgaccca ccgtgtgcag
     1861 caaggccacg gcggtgatgg acaagcccgc cagcgtgccg gcgaccgaca gcagttgcag
     1921 cagttcctcg cgcagccaca tgcggtcagg tgaacgggtc atcggggatg gcatggcgca
     1981 aaaaagacgt aacttagcag agcggcggcg cgccggggcg gggtctggca gtgacttcac
     2041 gcggccgaat tcggctttac atatcttcaa tcaagcgata accggcctgc cgcacgctgc
     2101 cgctacgatg cgtggctggc acggtggtcg cgctgccgac cagtggcggc gcgggctgcc
     2161 cgcgactcat aacgacagaa catgcaagcc caagaacctt cttccgagcc cgtggccgca
     2221 ccggccgcgg cgcccgccac ccgcccacgc cgctggctcg gcaccatcgt cacgctgctg
     2281 ctgatcgccg cgctggccgg cggcgcgtgg tggctggtgc agcgcgccaa ggcgcccagt
     2341 gccgcgg
//
LOCUS       DAOMVO010000364         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13480_length_2342_cov_13.801487, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000364 DAOMVO010000000
VERSION     DAOMVO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13480_length_2342_cov_13.801487"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKV30_12835"
     CDS             <1..486
                     /locus_tag="PKV30_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRL31135.1"
                     /translation="ADAAHDSALPERFDIVTALHACDTATDDAIAFGLAKKAQAMVLV
                     PCCQAELAAGLRQSKALQLSRTPLAELWRHPLHTREMGSQLTNVLRCLYLESQGYSVT
                     VTELVGWEHSLKNELIIARHTGQKKRSAGERLGALLDQFGLQALLPVRYPALVAAGDA
                     L"
     gene            complement(554..1423)
                     /gene="prmC"
                     /locus_tag="PKV30_12840"
     CDS             complement(554..1423)
                     /gene="prmC"
                     /locus_tag="PKV30_12840"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRL31136.1"
                     /translation="MSTKRKQLLILKYFDTSVANALGALQARSLPRPEAQMLLLHVLG
                     QPTHARAWLLAHDTDALPGAAHARLHELATRRLAGEPMAYLTGSKAFYGLQLQVDARV
                     LDPRDDTETLVDWALALLPRDAGRRVLDLGTGSGAIALALAAERPRARVTAVDASADA
                     LAVAAGNAARLGLELDLRHGDWFGPLADERFDLIVSNPPYIAADDPHLPALSHEPRQA
                     LVSGADGLHDIRRIIAAAPEHLAPGAWLLLEHGWDQAAAARALLSDSGWAQVQSRRDL
                     AGVERCSGGQWPL"
     gene            complement(1458..1895)
                     /locus_tag="PKV30_12845"
     CDS             complement(1458..1895)
                     /locus_tag="PKV30_12845"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HRL31137.1"
                     /translation="MRLPPSTSAPRLGDRLAHYRAVPRTLPAVACSMVMGATLLTAGC
                     TGGTPWGATPQNLPQVNLPRLENIPAHIDAGTNAGAQRLAERVRVALGAQPALADVRV
                     EGYEGGLIILSGAVPSPADRTQAVQTARQVVGVREVVDRMVGP"
     gene            complement(2035..>2342)
                     /locus_tag="PKV30_12850"
     CDS             complement(2035..>2342)
                     /locus_tag="PKV30_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRL31138.1"
                     /translation="GRSQHANKAKALQVLQARLQEKERSERAAKEAATRKGLIGSGDR
                     SDRIRTYNFPQGRLTDHRINLTLYKLALVMEGDLSDVIDALRAAREAEQMAELEGAG"
BASE COUNT          359 a          817 c          824 g          342 t
ORIGIN      
        1 gccgatgccg cgcacgacag cgcgctgccc gagcgcttcg acatcgtcac cgcgctgcac
       61 gcctgcgaca cggcgactga cgacgccatt gcctttggcc tggccaaaaa agcgcaggcc
      121 atggtgctgg tcccctgctg ccaggccgag ctggccgccg gcctgcggca gtcgaaggcg
      181 ctgcaactgt cgcgcacgcc gctggccgag ctgtggcgcc acccgctgca cacgcgcgaa
      241 atgggcagcc aactgaccaa cgtgctgcgc tgcctgtacc ttgaatcgca gggctacagc
      301 gtcaccgtga ccgagctggt cggctgggaa cacagcttga agaacgagct gatcatcgcc
      361 cgccacaccg ggcaaaaaaa gcgcagcgcc ggcgagcggc tcggggcgct gctggatcag
      421 ttcggtcttc aggcgctgct gccggtgcgc tatccagcgc tggtggcggc gggggacgcg
      481 ctataaaagg agaagctgat tgcgcacggc ggacgggctc tgcggcctga tttgatcagt
      541 attgagctga ccatcacaac ggccactgcc cacccgagca gcgctccacg cctgccagat
      601 cgcggcgcga ctgcacctgc gcccagcccg aatccgacaa caacgcgcgc gccgcggcgg
      661 cctgatccca gccatgctcc agcagcagcc aggcgccggg cgccaagtgt tctggcgcgg
      721 cggcgatgat gcggcgaata tcgtgcagcc cgtcggcgcc actcaccagc gcctggcgcg
      781 gctcgtgcga cagcgccggc aggtgcgggt cgtccgccgc gatgtagggc gggttgctga
      841 cgatgaggtc aaagcgctcg tccgccagcg ggccaaacca gtcgccgtgg cgcaagtcca
      901 gttccaaccc gagccgcgcc gcgttgcccg ctgcgacggc cagcgcgtcg gcactggcat
      961 cgacggccgt cacgcgcgcg cgcggccgct cagccgccag cgccagcgca atggcgccgc
     1021 tgccggtgcc cagatcgagc acgcggcggc ccgcatcgcg cggcagcaag gccagcgccc
     1081 agtcgaccag cgtctcggtg tcgtcgcgcg ggtcgagcac gcgggcatcg acctgcaact
     1141 gcagcccata gaacgccttg ctgcctgtga gataggccat cggctcgccg gccaggcggc
     1201 gcgtggccag ctcgtgcagg cgtgcgtggg cggcacccgg cagcgcgtcg gtgtcgtgcg
     1261 ccagcagcca ggcgcgcgcg tgggtgggtt gcccaaggac gtgcagcagc agcatctgcg
     1321 cttcagggcg cggcaggctg cgggcttgca gcgcccccaa tgcgttggcg actgatgtat
     1381 cgaagtactt caaaattaat agctgcttgc gcttggtgga caagcgctgg ggcctatttt
     1441 gatgcgcaga ccgctgatca aggcccgacc atgcggtcga ccacctcgcg cacgccgacc
     1501 acctggcgcg cggtctgcac ggcctgcgtg cggtcggcag gcgagggcac ggcaccgctc
     1561 aggatgatca ggccgccctc atagccctcg acgcgcacat ccgccagcgc gggctgcgcg
     1621 ccgagcgcga cgcgcacccg ctccgccagc cgctgcgcgc cggcgttggt gccggcgtcg
     1681 atgtgggcgg ggatgttctc caggcgtggc aggttcacct gcggcagatt ctgcggcgtc
     1741 gcgccccaag gcgtgccgcc ggtgcagccg gccgtcagca gcgtggcgcc catcaccatc
     1801 gagcaggcga cggcgggcag ggtgcgcgga acggctcggt agtgtgcaag gcggtcgccg
     1861 aggcggggcg cagaggtgga aggcggcaaa cgcatggtgg catttccttt tcgttcaagc
     1921 ggcggcgcgc gggccgcagc aatcgccggg atcgacgacg ccatgataag tgcggctaca
     1981 cgtggcagtc aggtggccgc gcgcgcaacg tcaaggcaac gcggacgcag gcgctcagcc
     2041 agcgccttcc agctctgcca tctgttccgc ctcgcgcgcg gcgcgcagcg cgtcgatgac
     2101 gtcggacaaa tcgccctcca tcaccagcgc cagcttgtac agcgtcaggt tgatgcggtg
     2161 atccgtcagc cgcccctgcg ggaagttgta ggtgcggatg cgatccgagc ggtcgcccga
     2221 gccgatcagg cccttgcgcg tggcggcttc cttggcggca cgctcgctgc gctctttttc
     2281 ctgcagccgc gcctgcagca cctgcagcgc cttggccttg ttggcgtgct ggctgcggcc
     2341 gt
//
LOCUS       DAOMVO010000365         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13526_length_2335_cov_15.263596, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000365 DAOMVO010000000
VERSION     DAOMVO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13526_length_2335_cov_15.263596"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKV30_12855"
     CDS             complement(<1..691)
                     /locus_tag="PKV30_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRL31139.1"
                     /translation="MHIHILGICGTFMGGVAALAREAGHRVTGCDAGVYPPMSDQLRA
                     LGIELIEGYGADQLPLKPDMFVIGNVVSRARLADGAPKFPLMEAILDAGAPYISGPQW
                     LLENVLQGRHVLAVAGTHGKTTTTSMLAWILERAGLSPGFLVGGVPLNFGISARLGTQ
                     AAGQQRSLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDVGQIERQF
                     HHLVRTVPASGR"
     gene            complement(785..2125)
                     /locus_tag="PKV30_12860"
     CDS             complement(785..2125)
                     /locus_tag="PKV30_12860"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL31140.1"
                     /translation="MNAVERDPTDHATPHQVVIVGGGAGGLELATRLGDTLAKRGRIA
                     VTLIDRNRAHVWKPKLHEIAAGSMDIDAHDLDYLAQAHWHHFQYRVGEMTGLDRAARE
                     VQVAPYIDNEGRQVTPARRIRYDTLVMAVGSQTNDFGTPGVAEHAIKLETAQDARRFN
                     RRLVNAAIRAHATEGDGPHHLRVAIIGAGATGVELAAELHRTMREVVSYGLERIDAEA
                     DLRIDLIEAADRVLPGLPPRLSAATLRLLQALGVQVHTSARVAAVDEGRVKLADGVVI
                     EADLIVWAAGVKAPEFLKDLDGLETNRINQLVVRPTLQTTRDEHIFAIGDCAACPWLG
                     TDKLVPPRAQAAHQQASLMLKQIGRVLDDQPLHDFQYRDFGSLVSLGEYSTVGNMMGA
                     LVGGDMVFEGMFARLMYRSLYTMHQLALHGWPKVLLDTLARTITRRTEPQVKLH"
     gene            complement(2138..>2335)
                     /locus_tag="PKV30_12865"
     CDS             complement(2138..>2335)
                     /locus_tag="PKV30_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HRL31141.1"
                     /translation="WSERPLACVAFKPGQSATPEELNAMLLQTHFAKWQLPERYEVID
                     AVPRTSTGKFWKLKLREQFPK"
BASE COUNT          364 a          783 c          775 g          413 t
ORIGIN      
        1 cgcggcccga cgccggcacg gtgcgcacca ggtgatgaaa ctgccgctcg atctggccca
       61 cgtcgtcaaa gatgtcggcg tggtcgaact ccaggttgtt cagcaccgcc gtgcgtgggc
      121 ggtagtggac gaacttgctg cgcttgtcga agaaggcggt gtcgtattcg tcggcctcga
      181 tcacgaagag cgaacgctgc tgtccggctg cttgcgttcc aagtcgggcg gatattccga
      241 aattgagcgg cacgccgccc accagaaagc ccggcgacag accagcgcgt tccagaatcc
      301 acgccagcat cgacgtggtc gtcgtcttcc catgcgtgcc ggccaccgcc agcacgtgcc
      361 gaccttgcag cacgttctcc agcagccact gcgggccgct aatgtagggc gcgccagcgt
      421 cgaggatcgc ttccatgagc ggaaacttgg gcgcgccatc ggccagccgc gcgcggctga
      481 ccacgttgcc gatgacgaac atgtcgggct tcaacggcag ttgatcggcg ccatagccct
      541 cgatcagctc gataccgagc gcgcgcaact ggtcgctcat cggcgggtag acacccgcgt
      601 cgcaaccggt gacgcggtga ccggcctcgc gcgccagcgc ggccacgccg cccatgaagg
      661 tgccgcaaat gcccaggatg tggatgtgca tgggccgaga ttctacggcc aatgttcaaa
      721 gccttttcgg gctttgacgc acgccggcag tgcgccagca gctattgttt tggtagtttc
      781 gcggtcagtg cagcttcacc tgtggctcgg tgcggcgcgt gatggtgcgc gccagcgtgt
      841 cgagcagcac cttgggccag ccgtgcagcg cgagctggtg catggtgtag agcgatcggt
      901 acatgagccg cgcgaacatg ccttcaaaca ccatgtcgcc gccgaccagc gcgcccatca
      961 tgttgcccac cgtgctgtat tcgcccagcg acacgagcga accgaagtcg cggtattgaa
     1021 agtcgtgcaa cggctggtca tcgagcacgc gcccgatttg cttgagcatc agcgatgcct
     1081 gctgatgcgc ggcctgcgcg cgcggcggca ccagcttgtc ggtgcccagc cacgggcagg
     1141 cagcgcagtc gccaatggca aagatgtgct cgtcgcgcgt ggtctgcagc gtgggccgca
     1201 ccaccagctg gttgatgcgg ttggtttcca gcccgtccag gtctttcagg aattcaggcg
     1261 ccttcacgcc cgccgcccag acgatcaggt cggcttcgat cacgacgcca tcggccagct
     1321 tcacgcggcc ttcgtccacc gccgccacgc gcgcgctggt gtgcacctgc acgcccagcg
     1381 cctgcagcag ccgcagcgtg gcggccgata agcgcggcgg caagccaggc agcacgcggt
     1441 cggcggcttc gatcaggtcg atgcgcaagt cggcctcggc gtcgatgcgc tccagcccgt
     1501 acgacacgac ttcgcgcatg gtgcggtgca gttcggccgc cagctccacg cccgtggcgc
     1561 cggcgccgat gatggccacg cgcaggtggt gcgggccgtc gccctcggtc gcgtgggcac
     1621 ggatggcggc gttgaccagc cggcggttga agcggcgcgc gtcctgcgcc gtctccagct
     1681 tgatggcgtg ctcggccacg ccgggcgtgc cgaaatcgtt ggtctggctg ccaaccgcca
     1741 tcaccagcgt gtcgtaacga atgcgtcgcg ccggcgtcac ctgccgacct tcgttgtcga
     1801 tgtagggcgc cacctgaacc tcgcgcgcgg cgcggtccag gccggtcatc tcacccacgc
     1861 ggtactgaaa gtggtgccag tgcgcctgcg caagatagtc cagatcgtgc gcatcaatgt
     1921 ccatgctgcc ggcggcgatc tcatgcagct tgggcttcca cacgtgggcg cggttgcggt
     1981 cgatcagcgt gacggcgatg cggccgcgct tggccagcgt gtcgcccagg cgcgtggcca
     2041 gttcaaggcc gcccgcaccg ccgccgacga tgacgacctg gtgtggggtg gcgtggtcgg
     2101 tggggtcgcg ttcgacagcg ttcatggtgc gtcccgccta tttcggaaac tgctcccgca
     2161 gcttcaactt ccagaacttc cccgttgatg tgcgcggcac cgcgtcgatc acctcatagc
     2221 gctcgggcag ttgccacttg gcgaagtgcg tttgcagcag catcgcgttc aattcttccg
     2281 gcgtcgcgct ctggcccggc ttgaaagcga cgcaggccag cggtcgctcg ctcca
//
LOCUS       DAOMVO010000366         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13619_length_2320_cov_10.659161, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000366 DAOMVO010000000
VERSION     DAOMVO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13619_length_2320_cov_10.659161"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKV30_12870"
     CDS             <1..360
                     /locus_tag="PKV30_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRL31142.1"
                     /translation="GEPRSGTGVAAGARASGEFDGVRGYQRGDPMKLIVWKKFAKSGE
                     LVSRDTVHMQRQELWLDFARAGALDAEGRLSRLTAWVLLADAQGLDYGLRLPGVEIKP
                     GSGAAQRLRCLEALATC"
     gene            497..>2320
                     /locus_tag="PKV30_12875"
     CDS             497..>2320
                     /locus_tag="PKV30_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HRL31143.1"
                     /translation="MTALAHLPREARDTLFLLAVIGWVILPQVAHLPIWASALAAGVL
                     VWRGWLAVTARPLPGRWWLIVLLVITLAGTWASHRTLLGRDAGVTLVVMLLTLKTLEL
                     RARRDALVVFFLGFFTMLTNFFYSQSLGVAAAMLIGLLGLLTALVNAHRPVGQPSLRE
                     SARMAGWMALAGAPVMVALFVLFPRFSPLWGIPSDALTGRSGLSSSMEVGNVAKLALD
                     DGIAMRVKFEGEPPPQRDLYFRGPVLSRFDGRNWTARGGGDGFEAAVGPGPADLQVSG
                     APIRYEVTLEPSNRPWLLTLDVAADAPELPANQRARMTADMQWLTYRPITDLLRYRAQ
                     SHPQFRYGLTGWNNRPRRDFAADLQLPPGFNPRTAALAEQLRQSVGDDPQALVRAALE
                     RLRTGGYTYTLEPGVNGQHTADEFWFDTKAGFCEHISSAFVVLLRAAGVPSRIVTGFQ
                     GGELNGVDGYWIVRNADAHAWAEVWYADRGWVRIDPTGAVMPGRIGQFQRLRAPEGFV
                     AGAIGTLSPTLIAQLRATWEAVNNRWNQWVLNYTQSRQFDLLKSLGFESPSWQDLGKL
                     IAGLLTVAALAGIAWARWERSQHDPWLRLLARARRQLAQAGV"
BASE COUNT          317 a          808 c          826 g          369 t
ORIGIN      
        1 ggcgagccgc gctcgggcac cggcgtggcc gcgggtgcgc gcgccagcgg cgaattcgac
       61 ggcgtgcgcg gctaccagcg cggcgacccg atgaagctga tcgtgtggaa aaaatttgcc
      121 aagtcgggcg agctggtcag ccgcgacacg gtgcacatgc agcggcaaga gctgtggctc
      181 gacttcgccc gcgccggcgc gttggacgcc gagggccgcc tgtcgcgcct gacggcctgg
      241 gtgctgctgg ccgacgcgca agggctggac tacggcctgc gactgccggg cgtcgagatc
      301 aagccgggca gcggcgcagc gcagcgcctg cgctgtcttg aggcgctggc gacatgctga
      361 tgaatgacga gatgacctcg gctggcgcct tcgatgacgg cgctgcgcgc catgaccgcg
      421 cacgggtcat ttcgtcatcg aggcggagcg aggacatttc gtcatgcgca ccaggggccg
      481 ccgcttgaaa aacccgatga ccgccctcgc ccacctgcca cgcgaagcgc gcgacacgct
      541 gtttttgctg gccgtgatcg gctgggtgat cttgccgcag gtggcgcatt tgccgatctg
      601 ggccagcgcg ctggcggctg gcgtgctggt gtggcgcggc tggctggccg tcaccgcgcg
      661 gccgctgccg gggcgctggt ggctgatcgt gctgctggtc atcacgctgg ccggcacctg
      721 ggcttcgcac cgcacgctgc tggggcgcga cgccggcgtc acgctggtgg tgatgctgct
      781 gacgctgaag acgctggagc tgcgcgcgcg acgcgacgcg ctggtggtgt tcttcctcgg
      841 ctttttcacc atgctgacca acttcttcta ttcgcagtcg ctgggggtgg cggcggcgat
      901 gttgatcggc ctgctcggct tgctgacggc gctggtcaac gcgcaccgcc cggtcggcca
      961 acccagcctg cgcgaatcgg cgcgcatggc cggttggatg gcgctggccg gcgcgccggt
     1021 gatggtggcg ctgttcgtgc tgtttccgcg cttctcgccg ctgtggggca ttccaagcga
     1081 cgcgctcacc gggcgcagcg ggctgtcgtc gagcatggaa gtcggcaacg tggccaagct
     1141 ggcgctcgac gacggcatcg ccatgcgcgt gaagttcgag ggcgagccgc cgccgcagcg
     1201 cgatctgtac tttcgcggcc cggtgctgag ccgcttcgac ggccgcaact ggacggcgcg
     1261 cggcggcggc gatggcttcg aggccgccgt cggccccggc ccggccgacc tgcaggtgtc
     1321 gggcgcgccg atccgctacg aagtcacgct ggagcccagc aaccgcccct ggctgctgac
     1381 gctcgatgtt gccgccgatg cgcccgagct gcccgccaac cagcgcgcac gcatgacggc
     1441 cgacatgcag tggctgacgt accgccccat caccgacctg ctgcgttacc gggcgcaaag
     1501 ccacccgcag ttccgctacg gcctgacggg ctggaacaac cggccgcggc gcgatttcgc
     1561 ggccgatctg caactgccgc ccggcttcaa cccacgcacc gcggcgctgg ccgagcaatt
     1621 gcgccagtcg gtcggcgacg acccgcaagc cctggtgcgc gccgcgctgg agcgcctgcg
     1681 caccggcggc tacacctaca cgctggagcc cggcgtgaac ggccagcaca cggcggacga
     1741 attctggttc gacaccaagg ccggcttttg cgagcacatc agctcggcct tcgtggtgct
     1801 gctgcgcgcc gctggcgtgc cctcgcgcat cgtcaccggc tttcagggtg gcgaactgaa
     1861 tggcgtggac ggctactgga tcgttcgcaa tgccgatgcc cacgcctggg ccgaggtctg
     1921 gtacgccgat cgcggttggg tgcgcatcga cccgaccggc gccgtcatgc ccgggcgcat
     1981 tggccagttc cagcgcctgc gcgcacccga gggctttgtc gccggcgcca tcggcacgct
     2041 cagcccgacg ctcatcgccc aactgcgcgc cacctgggaa gccgtcaaca accgctggaa
     2101 ccagtgggtg ctcaactaca cgcaaagccg ccagttcgat ctgctcaaga gcctgggttt
     2161 tgaaagcccc agctggcagg acttgggcaa gctcatcgcc ggcctgctga ccgtggcggc
     2221 gctcgccggc atcgcctggg cgcgttggga acgcagccag cacgacccct ggctgcgcct
     2281 gctggcgcgc gcgcgccgcc agctggcgca agccggcgtc
//
LOCUS       DAOMVO010000367         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13652_length_2317_cov_13.075155, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000367 DAOMVO010000000
VERSION     DAOMVO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13652_length_2317_cov_13.075155"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..968
                     /locus_tag="PKV30_12880"
     CDS             57..968
                     /locus_tag="PKV30_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL31144.1"
                     /translation="MSSLVSRWWDSDVGYSFRSSPTAIVAAAIALVCVFGAVFAHWVA
                     PHNPLDLTTLELSDARLPPAWSEGGSRKYLLGTDDQGRDILSALMYGARISLVVGVAS
                     VIISVIVGVALGLIAGFKGGWIDAFLMRVCDVMLSFPAILIALLIAGVGRALFPDANE
                     SLAFGVLIIAISLTGWVQYARTVRGSTMVERGKEYVQAARVTGVSNWRIMVKHVLPNV
                     LGPVMVLATIQVATAIITEATLSFLGVGVPPTSPSLGTLIRVGNDYLFSGEWWITIFP
                     GVMLVLIALSVNLLGDWLRDALNPRLR"
     gene            1161..2135
                     /locus_tag="PKV30_12885"
     CDS             1161..2135
                     /locus_tag="PKV30_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL31145.1"
                     /translation="MSLLQVKNLIVEFPSRRGNLRALDDISFDIAPGEILGVVGESGA
                     GKSLTGASIIGLLEPPGRVAGGEILLEGRRIDNLPHEQMRHVRGRQIGAIFQDPLTSL
                     NPLYSVGRQLIETIQTHLPLNADEARRRAIDLLKDTGIPAAEERIDHYPHQFSGGMRQ
                     RVVIALALAAEPKLIVADEPTTALDVSIQAQIIGLLKKVCRERGAAVMLITHDMGVIA
                     ETCDRVAVMYAGRIAEIGPVHEVINQPAHPYTEGLMAAIPDMALDREQLNQIDGAMPR
                     LNAIPRGCAFNPRCPQVFDRCRVERPDLIPAGATRAACWLHARQEVLA"
BASE COUNT          371 a          779 c          751 g          416 t
ORIGIN      
        1 ggccccagcg cttgtctggc aagcgcaagc agctataaat tgatgagtat ctaggcatga
       61 gcagtctggt atcccgatgg tgggacagcg atgtgggcta cagttttcgc agctcgccca
      121 cggccatcgt ggcggcggcg atcgcgctgg tctgcgtatt cggcgcggtg ttcgcccact
      181 gggtggcgcc gcacaacccg cttgatctga ccacgctcga actcagcgac gcgcgcctgc
      241 cgccggcctg gtcggaaggc ggcagccgca agtacctgct gggcaccgac gatcagggcc
      301 gcgacatttt gtcggcgctg atgtacggcg cgcgcatctc gctcgtcgtc ggcgtggcct
      361 cggtcatcat ctcggtcatc gtcggcgtgg cgctggggct catcgccggt ttcaagggcg
      421 gctggatcga tgccttcctg atgcgcgtgt gcgacgtgat gctgtccttc cccgccatcc
      481 tgatcgcgct gctgatcgcc ggcgtcgggc gcgcgctgtt tcccgacgcc aacgaatcgc
      541 tggcctttgg cgtgctgatc atcgccatct cgctcaccgg ctgggtgcag tacgcgcgca
      601 cggtgcgcgg cagcaccatg gtcgagcgcg gcaaggaata cgtgcaggcg gcgcgcgtga
      661 ccggcgtgtc caactggcgc atcatggtca agcatgtgct gcccaacgtg ctggggccgg
      721 tgatggtgct ggcgacgatt caggtggcca ccgcgatcat caccgaggcc acgctgtcct
      781 tcctcggcgt cggcgtgccg cccacctcgc catcgctcgg cacgctgatc cgcgtcggca
      841 acgattacct gttctcgggc gaatggtgga tcacgatttt tccgggtgtc atgctggtgc
      901 tgatcgcgct cagcgtgaac ctgctgggcg attggttgcg cgatgcgctg aatccgaggc
      961 tgcggtgagg tcagagcctc aacacccgga cgcgaaggac gcgaaagttg cgcgaaaggc
     1021 gctaaagaac tgcaggttga atttcttgcg ctcttcgcga aatcttcacg cccttcgcgt
     1081 atggctttca ggttttctgc cctccagcgc tttcccatca agcgcaagca gctattaaat
     1141 tcatagttac cacagagccc atgtcgcttc tgcaagtcaa gaacctgatc gtcgaattcc
     1201 ccagccggcg cggcaacctg cgcgcgctgg acgacatctc cttcgacatc gcgcccggcg
     1261 aaattctggg cgtggtgggc gaatcgggcg cgggtaagtc gctcacgggc gcctccatca
     1321 tcggcttgct ggagccaccg ggccgcgtcg cgggcggcga gatattgctg gaaggccgcc
     1381 gcatcgacaa cctgccgcac gagcagatgc gccatgtgcg cgggcgccag atcggcgcca
     1441 tctttcaaga cccgctgacc tcgctcaacc cgctgtattc ggtcggccgc cagctgatcg
     1501 agaccattca gacgcatctg ccgctgaacg ccgacgaggc gcgccgccgc gccatcgacc
     1561 tgctgaaaga cactggcatt cccgccgccg aagagcgcat cgaccactac ccgcaccaat
     1621 tcagcggcgg catgcgccag cgcgtggtga ttgccctggc gctggcggcc gagcccaagc
     1681 tgatcgtcgc cgacgagccg accaccgcgc ttgatgtgtc gatccaggcg cagatcattg
     1741 gcctgttgaa aaaagtgtgc cgcgagcgcg gcgcggcggt gatgctgatc acgcacgaca
     1801 tgggcgtgat cgccgaaacc tgcgaccgcg tggccgtgat gtacgccggc cgcattgccg
     1861 agatcggccc ggtgcacgaa gtcatcaacc agccggcgca cccgtacacc gaagggctga
     1921 tggcggcgat ccccgacatg gcgctcgacc gcgaacagct caaccagatc gacggcgcca
     1981 tgccgcgcct gaacgccatt ccgcgcggct gcgccttcaa tccgcgctgc ccgcaggtgt
     2041 ttgaccgttg ccgcgtcgaa cggcccgact tgataccggc tggcgccacg cgcgccgcct
     2101 gctggctgca tgcgcggcag gaggtgttgg catgagcggc gggcgtcacg gtgttttctc
     2161 cttcccccgc tgggggaagg ccgggatggg gcccaccctg gtcgaatccg gcgccgccgg
     2221 cccccacgcc aaccctcccc cagcggggga gggcgcaggt caacccctgg tgcgcgccga
     2281 agccctggcc aagaccttcg acgtctcgcc gccgctg
//
LOCUS       DAOMVO010000368         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13688_length_2312_cov_9.490031, whole genome shotgun sequence.
ACCESSION   DAOMVO010000368 DAOMVO010000000
VERSION     DAOMVO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13688_length_2312_cov_9.490031"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKV30_12890"
     CDS             complement(<1..1075)
                     /locus_tag="PKV30_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRL31146.1"
                     /translation="MNHDKPVTQTIGHLIDGQIVNDTARTQAVFNPATGQVSGHVALA
                     SKATVEQAIASAEKAFPEWRNTPPLKRARVMSKLKVLLEQNADKIAAMITAEHGKVLN
                     DAHGELQRGIENVEYASYAPELLKGEHSRNVGPGIDSWAEHQALGVTAGITPFNFPAM
                     VPLWMWPMAVACGNTFVLKPSERDPSSALFIAQLALEAGLPPGVLNVVNGDKEAVDTL
                     LHSPAVKAVSFVGSTPIAEYIYSEGTKHGKRVQALGGAKNHAVVMPDADIANAVNAMM
                     GAAYGSCGERCMAVPLIVAVGDEVAEKMIAGLKTEIANMKVGPGTDNANDMGPLVTKP
                     HFEKVTGYVDQGVKEGATLLVDGR"
     gene            complement(1084..1272)
                     /locus_tag="PKV30_12895"
     CDS             complement(1084..1272)
                     /locus_tag="PKV30_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31147.1"
                     /translation="MRLKSLPDWLLKDLPDGDVAALQRLLGTKVYVHSLDSHGYLWLG
                     SEPDQQWFCVELSCIVFS"
     gene            complement(1339..1629)
                     /locus_tag="PKV30_12900"
     CDS             complement(1339..1629)
                     /locus_tag="PKV30_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HRL31148.1"
                     /translation="MATHIAPFDAADYLDDGHTIAEYLTAALEDPNPDVFLTAVRDVA
                     RARGMAQLAKDAGLGRESLYKALTPGAKPRYDTVLKLLNALGVRLSATPVQS"
     gene            complement(1632..1931)
                     /locus_tag="PKV30_12905"
     CDS             complement(1632..1931)
                     /locus_tag="PKV30_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRL31149.1"
                     /translation="MNTFLRTEVFDVWLSALKDKIGRARITHRIRSAEHGNFGDCEPV
                     GDGVFEMRVHVGPGYRVYYTRRGQVIYLLLLGGDKSTQKRDIKRAIEMARELDKE"
     gene            complement(1959..>2312)
                     /locus_tag="PKV30_12910"
     CDS             complement(1959..>2312)
                     /locus_tag="PKV30_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL31150.1"
                     /translation="PVACAAGIAALDLLEREDGVGRVKALAPHFEAAVHSLKGAKHVL
                     DIRNYGLAAGFTIEALPGEPAKRPYEIAMHCWKNGFYVRYGGDTIQLAPPFISTPAEI
                     DRLVGALGEALDAVA"
BASE COUNT          390 a          766 c          720 g          436 t
ORIGIN      
        1 cgcgcccatc gaccaacagc gtcgcgcctt ccttcacgcc ctggtcgacg tagccagtca
       61 ccttctcgaa atgcggcttg gtcaccagcg gccccatgtc gttggcgttg tcggtgccgg
      121 ggccgacctt catattagcg atctcggtct tcagcccggc gatcatcttc tccgccacct
      181 cgtcgcccac ggccacgatc agcggcaccg ccatgcagcg ctcgccgcac gagccgtagg
      241 ccgcgcccat catggcgttc accgcgttgg cgatgtcggc gtcgggcatc accacggcgt
      301 ggttcttggc gccgcccagc gcctgcacgc gcttgccatg cttggtgccc tcgctgtaga
      361 tgtattcggc aatcggcgtc gagccgacga agctgacggc cttcacggcg ggagagtgca
      421 gcagcgtatc gacggcttcc ttgtcgccgt tcaccacgtt cagcacgccc ggcggcaggc
      481 cggcctccag cgcgagctgc gcgatgaaca gcgcgctgct cggatcgcgc tccgaaggct
      541 tcagcacgaa ggtgttgccg caggccaccg ccatcggcca catccacagc ggcaccatgg
      601 ccggaaagtt gaacggcgtg atgccagccg tcacgcccag cgcctggtgc tcggcccacg
      661 agtcgatgcc gggccccacg ttgcgcgagt gctcgccctt gagcagttcg ggcgcgtagc
      721 tcgcgtattc gacgttctcg atgccgcgct gcaactcacc atgcgcgtcg ttcagcacct
      781 tgccgtgttc ggcggtgatc atggcggcga tcttgtcggc attctgctcc agcagcacct
      841 tcagtttgct catcacccgc gcgcgcttga gcggcggcgt gttgcgccat tccggaaacg
      901 ccttctcggc cgaggcaatg gcctgctcga cggtggcctt gctcgccagc gccacatggc
      961 cgctgacttg gcccgtggcg gggttgaaca cggcttgagt gcgcgccgtg tcgttgacga
     1021 tctggccgtc gatgaggtgg ccgatggttt gggtgacggg tttgtcgtgg ttcataaaca
     1081 gtctcaagaa aaaacaatgc aggaaagctc aacgcaaaac cactgctgat ctggctcact
     1141 gcctaaccat aaatagccgt gggaatccag cgaatggacg tagaccttag tgccgagcaa
     1201 tctttgcaaa gcggcgacat cgccatccgg caagtccttc agcagccaat ctggaagcga
     1261 tttcaggcgc acgcgagagc ctggcgcgag tgaatgagca gccatagcgg gcatgctctc
     1321 aaagcgtcgc actagaactc atgactgaac aggcgtcgcc gaaagcctga cgccaagcgc
     1381 gttcaacagc ttgagcaccg tgtcatagcg cggcttggct cccggcgtca gcgccttgta
     1441 aaggctttcc cgccccagcc ccgcgtcctt ggccagttgc gccatgccac gtgcgcgcgc
     1501 cacgtcgcgc accgccgtca gaaacacgtc ggggttcggg tcttccaatg cggcggtcag
     1561 gtattcggcg atggtgtgcc catcgtccag atagtctgca gcgtcgaagg gtgcgatgtg
     1621 cgtcgccatg ttcactcctt gtccagttcg cgcgccatct cgatggcccg cttgatgtcg
     1681 cgcttctggg tcgacttgtc gccgcccagc agaagcaggt agatcacctg gccccggcgc
     1741 gtgtagtaaa cgcggtaacc ggggccgaca tgcacgcgca tctcgaaaac gccatcgcct
     1801 actggctcgc aatcgccaaa attgccgtgc tcggcggagc ggatgcggtg ggtaatgcgg
     1861 gcccgtccta tcttgtcctt cagcgcggag agccaaacgt caaagacctc cgtccgaaga
     1921 aaggtgttca tgcgcgcatc gtatccgatt ggatactttt acgccactgc gtccaacgcc
     1981 tcccccagcg cgcccaccag ccgatcaatc tccgccggcg tgctgatgaa cggcggtgcg
     2041 agctggatgg tgtcgccgcc gtagcgcacg tagaagccgt tcttccagca gtgcatggcg
     2101 atctcgtagg ggcgtttggc aggctcgccg ggcaatgcct cgatggtgaa gccggcggcc
     2161 aggccgtagt tgcggatgtc aagcacgtgc ttggcgccct tgaggctgtg cacggcggct
     2221 tcgaagtgcg gcgccagagc cttgacgcga cccacgccgt cctcgcgctc cagcaggtcg
     2281 agcgcggcga tgccggcggc gcaggccacc gg
//
LOCUS       DAOMVO010000369         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13742_length_2303_cov_13.812722, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000369 DAOMVO010000000
VERSION     DAOMVO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13742_length_2303_cov_13.812722"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="dxr"
                     /locus_tag="PKV30_12915"
     CDS             complement(<1..779)
                     /gene="dxr"
                     /locus_tag="PKV30_12915"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRL31151.1"
                     /translation="MKKQRVAVLGSTGSIGVNTLDVIARHPDRFEVVALTAATQVDVM
                     LAQCVQFKPRVAVMASAPHARELAEKIKQKGLTTQVDQAPEAIESVASSGEVDTVMAA
                     IVGAAGLAPCLAAARAGKRLLLANKEALVVGGDVFMNAVREGDATLLPIDSEHSAIFQ
                     CLPEDASQWARRIDRIMLTSSGGPFRQTPLADLPAMTPSQACAHPNFAMGRKISVDSA
                     TMMNKALEVIEARWLFDLPPERIEVVIHPQQIIHSMVQFHDA"
     gene            complement(779..1648)
                     /locus_tag="PKV30_12920"
     CDS             complement(779..1648)
                     /locus_tag="PKV30_12920"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL31152.1"
                     /translation="MLRQRIITAVIMLAVLVAALWSASPVPFIVLALVMMAAAAWEWA
                     RLNGVQGAGALALGAAMVLACGLSWWAGWVNEPFRAWWLVVALWVVVGGLLISVGVDG
                     WSRVPAFLRLALGPAALWVAWLAVVQARMVGVNFLLSVLALVAAADIAAYFAGRAFGG
                     QLVARKLAPSISPNKSWEGVFGGMAGVLLLAALWIFFDRQIAPADWSRSLYSHLWQGG
                     GLVWLVPSVMALAGLSVVGDLVESLVKRAAGAKDSSQLLPGHGGVLDRIDAQLPTLPF
                     AMALVTLVAQRVA"
     gene            complement(1650..2300)
                     /gene="uppS"
                     /locus_tag="PKV30_12925"
     CDS             complement(1650..2300)
                     /gene="uppS"
                     /locus_tag="PKV30_12925"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRL31153.1"
                     /translation="MPRIAGHRQGVESLRRCIRACVKRGVGVLTVFAFSSENWGRPQD
                     EVSGLMELMGKALVREVPELKEAGIRLHFVGDRQGLPERMRQSLTQAEAATADNRGMV
                     LNVCFNYGGRWDIAQAAAQLAARGEPITENALDRALALSHGPDPDLLIRTGGEQRISN
                     FLLWQCAYSELYFSDRLWPDFDEAALDEAFAAFAERERRFGKTSEQVAPASRAAAH"
BASE COUNT          423 a          853 c          706 g          321 t
ORIGIN      
        1 gcatcgtgaa actgcaccat cgaatggatg atctgctgcg ggtgaatcac cacctcgatg
       61 cgctcgggcg gcaggtcgaa cagccagcgc gcctcgatca cctccagcgc cttgttcatc
      121 atggtggccg agtcgaccga gatcttgcgg cccatggcga agttggggtg cgcgcaggcc
      181 tggcttggcg tcatggccgg caagtcggcc agcggcgtct gccggaacgg gccgccggat
      241 gaggtgagca tgatgcggtc gatgcggcgc gcccactggc tggcatcctc gggcaggcac
      301 tgaaagatgg ccgaatgctc gctgtcgatg ggcagcagcg tggcatcgcc ctcgcgcacg
      361 gcgttcatga acacgtcgcc gccgaccacc agcgcttcct tgttggccag cagcagccgc
      421 ttgccggcgc gcgctgcggc caggcagggg gccaggccgg cggcgccgac gatggcggcc
      481 atcacggtat caacttcgcc cgaagacgct acgctttcaa tagcttccgg cgcttgatcc
      541 acctgcgtcg tcagcccttt ttgcttgatt ttctcggcca gctcgcgggc gtgcggcgca
      601 ctcgccatca ccgccacgcg cggcttgaat tgcacgcact gcgccagcat gacatcgacc
      661 tgcgtggcag ccgtgagcgc gacgacctcg aagcgatccg ggtggcgcgc gatcacatcc
      721 agcgtgttga cgccaatcga cccggtggag ccgagcacgg ccacgcgctg ctttttcatc
      781 acgccacgcg ctgagccacc agggtgacca aggccatcgc aaaaggcagc gtcggcaact
      841 gggcgtcgat gcggtccagc accccgccgt gcccgggcag cagctggctc gaatccttgg
      901 cgccagcggc gcgcttgacc agcgactcga ccagatcgcc caccacgctc aagcccgcca
      961 gcgccatgac actgggcacc agccacacca agccgccgcc ttgccacagg tgggaataca
     1021 ggctccggct ccaatcagcg ggcgcgattt gccggtcgaa aaagatccac agcgccgcca
     1081 gcagcagcac gccggccatg ccgccgaaca cgccttccca gctcttgttg gggctgatcg
     1141 acggcgccag cttgcgcgct accagttggc cgccaaacgc acgaccggcg aagtaggctg
     1201 cgatgtcggc ggccgcgacc agcgccagca ccgagagcag aaaattcacg cccaccatgc
     1261 gggcctgcac caccgccagc caggccaccc acagcgccgc cgggcccagt gccagtcgca
     1321 ggaacgccgg cacgcgcgac cagccgtcga caccgacgct gatgagcagg ccacccacga
     1381 caacccacag cgccaccacc agccaccagg cacggaaggg ctcgttcacc caacccgccc
     1441 accaactgag cccacaagcc agcaccatgg ccgcgccaag ggccagggcg ccagcgccct
     1501 gcacgccgtt gagccgcgcc cattcccagg ccgcagccgc catcatcacc agcgccagca
     1561 cgatgaaggg cacgggcgaa gccgaccaca gcgccgccac cagcacggcc agcatgatca
     1621 cagcggtgat gatgcgctgg cgcagcatgt cagtgggctg ccgcgcgcga tgcgggcgcg
     1681 acctgttccg acgtcttgcc gaaacgccgc tcgcgctcgg caaacgcggc aaaggcttcg
     1741 tcgagcgccg cttcgtcgaa atcgggccac agccgatcgc tgaaatacag ctcgctgtac
     1801 gcgcattgcc acagcaggaa attgctgatg cgctgctcgc cgccggtgcg gatcagcaga
     1861 tcgggatcgg gcccgtgcga cagcgccaga gcgcgatcca gtgcgttttc agtaatcggt
     1921 tcgccgcgcg cggccaactg cgccgccgcc tgcgcgatgt cccagcggcc gccatagttg
     1981 aagcacacgt tcagcaccat gccgcgattg tcggccgtgg ccgcctcggc ctgcgtcagg
     2041 ctttgccgca tgcgctcggg caagccctgc cggtcgccga cgaaatgcag gcgtatgccc
     2101 gcctccttca attcgggcac ctcgcgcacc agcgccttgc ccataagctc catcaagcca
     2161 ctcacctcgt cttgcggacg gccccagttt tccgacgaaa acgcaaacac cgtcagcacc
     2221 cccacgccgc gcttcacaca ggcgcgaatg cagcgccgca gagactccac gccctgccga
     2281 tgccccgcaa tgcgcggcat cag
//
LOCUS       DAOMVO010000370         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13749_length_2302_cov_15.187806, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000370 DAOMVO010000000
VERSION     DAOMVO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13749_length_2302_cov_15.187806"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKV30_12930"
     CDS             <1..770
                     /locus_tag="PKV30_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL31154.1"
                     /translation="YDSLAAWRATTERLTSFEAALSKENQAAAPVHTAPAAMDIEVTD
                     LTLQLPTGATLLQGATLRALPGQHTLVQGPSGSGKSTLFRALAGIWPWAQGRVALPAR
                     TMFIPQRPYFPDGALRDALAYPEPASAYTDEQLRTALHDALLPALAERLDERDNWTQK
                     LSGGEQQRLAIARVLLKQPAWVLADEATSALDVPAEQALYTRLKAMTEHAGGALLSIA
                     HRPSVAAFHAQRWQLEPAAPDGAARYRLSTGPLAVPA"
     gene            complement(834..1358)
                     /locus_tag="PKV30_12935"
     CDS             complement(834..1358)
                     /locus_tag="PKV30_12935"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRL31155.1"
                     /translation="MALALALGVGAASGAAAQGVDWTLTGGEAKKADVKKLGVIAGWT
                     RAEPLWQGQQWRLKLRHEVELSAWDVPKASDLVEFGYSPVFRIERPFSGGTSAFFVEA
                     SIGVRLLSHTRVSPERTLSTAFQFSDQLGVGVQWGRDARSTLGVRYQHISNAGIKKPN
                     PGMDFVLLYYTHRF"
     gene            complement(1648..>2302)
                     /locus_tag="PKV30_12940"
     CDS             complement(1648..>2302)
                     /locus_tag="PKV30_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL31156.1"
                     /translation="VLSDLAGSDGAARAQALDGQGGRCLFMPCDVTSTEQVDALVANT
                     VQQWGSVDIVFANAGIGGLADTTDYTDPDYLRTIDINLNGVFRTARAALRQMYRQGSG
                     SIINCASILGTLGQSGAVAYSAAKGGVVNMTRTLAIEAAPRGVRVNSIGPGYIDTPLL
                     KDLPDEMRQALIRLHPIGRLGRDEEVANAVLFLGSDEASFVTGAYLLVDGGYAAGKS"
BASE COUNT          358 a          828 c          796 g          320 t
ORIGIN      
        1 actacgacag cctggccgcc tggcgcgcca ccaccgagcg gctgaccagt ttcgaggccg
       61 cgctttcaaa ggaaaatcag gctgcagcgc ccgtccatac tgcgccagca gctatggata
      121 ttgaagtaac cgacctgacc ctgcagctgc ccaccggcgc caccctgctg caaggcgcca
      181 cgctgcgcgc cctgcccggc cagcacacgc tggtgcaggg gccgtcgggc agcggcaaga
      241 gcacgctgtt ccgcgcgctg gccggcatct ggccctgggc gcaggggcgg gtggcgctgc
      301 ccgcgcgcac catgttcatc ccgcagcgcc cgtacttccc ggacggcgcg ctgcgcgacg
      361 ccctggccta ccccgagcct gccagcgcct acaccgacga acagctcagg accgcgctgc
      421 acgacgccct gctgcccgcc ctggccgaac ggctggatga gcgcgacaac tggacgcaga
      481 agctgagcgg cggcgagcag caacgcctgg cgatcgcgcg cgtgctgctg aaacaaccgg
      541 cctgggtgct ggccgacgaa gccaccagcg cactcgacgt gccggccgag caagccctgt
      601 acacgcggct gaaggccatg accgagcacg ccggcggcgc cctgctgtcc atcgcccacc
      661 ggcccagcgt ggcggcgttc catgcgcagc gctggcagct ggagcctgcc gcgccggatg
      721 gcgccgcgcg ctatcggctc agcaccgggc cgctggccgt gccggcttga accggcggaa
      781 aacgttgcgt tggcgcgcgg gtactgcgca accagctatc cgatcgatag caatcagaag
      841 cggtgcgtgt agtacagcag cacgaagtcc atgcccgggt tcggcttctt gatgccggcg
      901 ttggagatgt gctggtagcg cacgcccagc gtcgagcgcg cgtcgcgccc ccactgcacg
      961 cccaccccca gctggtcgct gaactggaag gccgtgctga gcgtgcgctc gggcgacacg
     1021 cgggtgtgcg acagcaggcg cacgccgatc gaggcttcga cgaagaaggc gctggtgccg
     1081 cccgagaacg gacgctcgat gcggaacacg ggggaatagc cgaattcgac caggtcgctg
     1141 gccttgggca cgtcccaggc ggacagttcg acctcgtgcc gcagcttcag gcgccactgc
     1201 tggccctgcc acagcggctc ggcccgcgtc cagccggcga tgacgcccag cttcttgacg
     1261 tcggccttct tcgcctcgcc gccggtcagc gtccagtcga cgccttgcgc cgcggcaccg
     1321 ctggcggcgc cgacccccaa ggccagcgcc agcgccactc cggtcaagcg cctgcccatc
     1381 ggcaacgcgg gcggtgcaga agtatggaag cgcgagccat gagcaggcgt gcggtgccga
     1441 aatgagcgca tagtgttgtt cgcgttgtgg gtgaatggcg gccggccagc ggctccggcg
     1501 ccgcagtgtg cccgaacaaa aaaagccttg ccaggcgcac ggccagcagg atggcatttt
     1561 tagcccgccg cgcctgccgg gcaatggccc tgtgcgctcg cggcattggc cacacacgtg
     1621 cgcggcccgg gccgggccgc agggcgatca ggacttgccg gccgcgtagc cgccgtccac
     1681 cagcaggtac gccccggtga cgaagctggc ctcgtcgctg ccgaggaaca gcacggcgtt
     1741 ggccacttcc tcgtcgcggc ccaggcggcc gatggggtgc agccggatca gggcctggcg
     1801 catctcgtcc ggcaggtcct tgagcagcgg cgtgtcgatg tagccggggc cgatggagtt
     1861 gacgcgcacg ccgcgcggcg ccgcctcgat ggccagcgtg cgcgtcatgt tgaccacgcc
     1921 gcccttggcc gccgaatagg ccacggcgcc gctctgcccc agcgtgccca ggatcgacgc
     1981 gcaattgatg atgctgcccg aaccctgacg gtacatctgc cgcagcgccg cgcgcgcggt
     2041 gcggaacaca ccgttcaggt tgatgtcgat ggtgcgcagg taatcggggt cggtgtagtc
     2101 cgtggtgtcg gccaggccgc cgatgccggc gttggcgaac acgatgtcga cgctgcccca
     2161 ctgctgcacg gtgttcgcca ccagcgcatc gacctgctcg gtcgaggtca cgtcgcaggg
     2221 catgaacagg cagcggccgc cctgcccatc cagcgcctgg gcgcgcgcgg cgccgtcgct
     2281 gccggccaga tcggacagca cc
//
LOCUS       DAOMVO010000371         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13847_length_2290_cov_8.802685, whole genome shotgun sequence.
ACCESSION   DAOMVO010000371 DAOMVO010000000
VERSION     DAOMVO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13847_length_2290_cov_8.802685"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKV30_12945"
     CDS             <1..291
                     /locus_tag="PKV30_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRL31157.1"
                     /translation="SYKQAKLTIHTENLTIFDEILAGRQDIFVTEAAEALVMQRQKPG
                     LCAVNPDKPLQYGEIAFLLPRGDTVMKQFVDQWLHLARASGEYAGIENRWLR"
     gene            360..1823
                     /locus_tag="PKV30_12950"
     CDS             360..1823
                     /locus_tag="PKV30_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL31158.1"
                     /translation="MFLACGMVVAQADERVVVGSKRFTESYILGEIVAQTARTTGVRA
                     EHRPGLGNTAVVLEALKAGSIDIYPEYLGTIEQEILKLPAGSAPETIARELARLGLAH
                     GVPLGFSNSYALATTEALAGQRGLRRISDLRAQPDLRVALSQEFLGRADGWPGLAARY
                     GLPQRPSAIDHGLSYAALATARIDVTDIYTTDARIAELKLHTLQDDAHYFPRYDAVLL
                     YRADLPQRAPLAWQRIAGLQGRIDTARMIAMNAEAELQGRSFAQIAAGFLSGDAGTVA
                     TPSAHSSFWQRLTADDLGRLTRQHLLLVAVSLALATLIGVPLGALAAAWRPISGPVMA
                     VVGLMQTIPSLALLAALIPLLGRIGTVPAIVALTLYALLPIVRNTATGLEQVPRALKD
                     AGLALGMTRAQCFRLVDLPLAAPVLLAGLKTAAVLTVGTATIAAFIGAGGYGERIAQG
                     LALNDGAMLLAGAVPSAALALLTQGAFALLERWAARR"
     gene            2010..>2290
                     /locus_tag="PKV30_12955"
     CDS             2010..>2290
                     /locus_tag="PKV30_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRL31159.1"
                     /translation="MTFAPLQNDTFLRACLGQATPHTPVWLMRQAGRYLPEYCATRAQ
                     AGSFMGLATNVDYATEVTLQPLARYPLDAAILFSDILTVPDAMGLGLSF"
BASE COUNT          328 a          807 c          759 g          396 t
ORIGIN      
        1 agctacaagc aggccaagct gacgattcat accgagaacc tgaccatctt tgatgaaatc
       61 ctggccggcc ggcaggacat cttcgtcacc gaggcggccg aggcgctggt gatgcagcgc
      121 cagaagccgg gcctgtgcgc cgtcaacccc gacaagccgc tgcaatacgg cgagatcgcc
      181 tttttgctgc cgcgcggcga cacggtgatg aagcagtttg tcgaccagtg gctgcacctg
      241 gcgcgcgcca gcggcgaata cgccggcatc gagaaccgct ggctgcgttg atcttcgcta
      301 tttcaagtca aacaggccgc cagcgcttgc ctggcgtgcg caaggcgctc ctgtttttga
      361 tgttcctggc ctgcggcatg gtggttgcgc aggccgacga gcgcgtggtg gtcggctcca
      421 agcgctttac cgaaagctac atcctgggcg agatcgtcgc gcaaacggcg cgcacaaccg
      481 gcgtgcgcgc cgaacaccgg ccgggcctgg gcaacacggc ggtggtgctg gaggcgctca
      541 aggccggctc gatcgacatt taccccgaat acctgggcac catcgagcag gaaatcctca
      601 agctgcccgc cggcagcgcg cccgaaacca tcgcgcgcga actggcgcgc ctggggctgg
      661 cgcacggtgt gccgctgggt ttttccaaca gctacgcgct ggccaccacc gaggcgctgg
      721 cgggccagcg cgggctgcgc cgcatctccg acctgcgcgc gcagcccgat ctgcgggtgg
      781 cgctgtcgca agaatttttg ggccgcgccg acggctggcc cgggctggcc gcgcgctacg
      841 gcctgccgca gcgcccgagc gccatcgacc acggcctgtc gtacgccgcg ttggccaccg
      901 cgcgcatcga cgtcaccgac atctacacca ccgacgcgcg cattgccgag ctgaagctgc
      961 acacgctgca ggacgacgcg cactactttc cgcgctacga cgccgtgctg ctgtaccgcg
     1021 ccgatttgcc gcaacgcgcg ccgctggcgt ggcagcgcat cgccgggctg caaggccgca
     1081 tcgacaccgc gcgcatgatc gccatgaacg ccgaggccga attgcaaggc cgctccttcg
     1141 cgcagattgc ggccggcttt ctgtctggcg atgccggcac ggtcgccacc ccaagcgcgc
     1201 attcgagctt ctggcagcgc ctcacggccg acgacctggg ccgcctgacg cgccagcacc
     1261 tgctgctggt ggccgtgtcg ctggcgctgg ccacgctgat cggcgtgccg ctgggcgcgc
     1321 tggcggcggc gtggcggccg atcagcgggc cggtgatggc ggtggtcggg ctgatgcaga
     1381 ccatcccttc gctcgcgctg ctggcggcgc tgattccgct cttgggccgc atcggtaccg
     1441 tgcccgccat cgtcgcgctg acgctctacg cgctgctgcc catcgtgcgc aacaccgcca
     1501 ccgggctgga gcaggtgccg cgcgcgctga aggatgccgg cctggcgctg ggcatgacgc
     1561 gcgcgcagtg ttttcggctg gtcgatctgc cgctggcggc gccggtgctg ctggccggcc
     1621 tgaagaccgc cgccgtgctg acggtgggca cggcgacgat tgccgccttc atcggcgccg
     1681 gcggctatgg cgagcgcatc gcccaaggcc tggcgctgaa cgacggcgcc atgttgctgg
     1741 ccggcgccgt gccctcggcc gcgctggcgc tgctgacgca aggcgccttc gcgctgctgg
     1801 agcgctgggc ggcgcggcgc tgaattcagt tactctgttt ttgatagctg cttgcgctgg
     1861 attgacaagc gctggcggcc gatttgattt gaagcccacc tcgagcagct tttttggctg
     1921 ttctttcgcg tccttcgcga atcctttgcg tccttcgcgt ccggtcttcg gttttcggcg
     1981 ccccacgaca acactgagac aatcgcccca tgactttcgc cccgctccaa aacgacactt
     2041 tcctgcgcgc ctgcctgggc caggccacac cgcacacgcc cgtgtggctg atgcgccagg
     2101 ccgggcgcta cctgcccgaa tactgcgcca cgcgcgccca ggccggcagc ttcatgggtc
     2161 tggccaccaa cgtcgattac gcgaccgagg tcacgctgca accgctggcg cgctacccgc
     2221 tggatgcggc cattctgttc agcgacatcc tcaccgtgcc cgatgcgatg ggcctgggcc
     2281 tgtcgtttca
//
LOCUS       DAOMVO010000372         2286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_13873_length_2286_cov_12.188256, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000372 DAOMVO010000000
VERSION     DAOMVO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13873_length_2286_cov_12.188256"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..644
                     /locus_tag="PKV30_12960"
     CDS             105..644
                     /locus_tag="PKV30_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRL31160.1"
                     /translation="MPALLGAAVGMLARWSQPRLAHALARLGNPLVQTLAGTALFALL
                     AAVVPLVRFSGHHELVHALAHGVVAGAAALLGLAVLKALAMALCLASGWRGGEIFPLL
                     FAGAAAGLAAVHALPGLPVTVALVAGMTAAATVGMGKPVAVLLIMLLLTGASAPGTLC
                     VGAFVGYGAARLLPARAGH"
     gene            complement(728..2275)
                     /locus_tag="PKV30_12965"
     CDS             complement(728..2275)
                     /locus_tag="PKV30_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL31161.1"
                     /translation="MRADHVTTPAAAPLIKALRRTLGAGTELVETHISWLLLSGADAY
                     KLKKPVKLDFLDFSRLEQRRAACDEELRINRRTAPDLYMRVLPVTGSPDAPQLDGSGE
                     PIDWAVHMRRFPADALLEGRAARGELQAEHIDALAREIAAFQSRADVAPPGSHYGDAA
                     QLAELAQDNFKPLLGLQRLSGKREQLLKLQHWTRTEGTRLAPLMAARRAAGHVREGHG
                     DLHLGNLLWLDGRPLLFDAIEFDPRLRWIDTACDIAFLFMDLHAHGLAPLAWRFLNAW
                     LEQTGDYEAVQRLPHDAVYRALVRAKVAALCVAQGDDAALAEVERYVQLAAELAAPRQ
                     PWLALTLGVSGSGKSSQTQSLIEQRGLVRVRADVERKRLFGLAPEASSAGVPGGIYSE
                     ATSDRVFARLLEVARGALQAGQPVVVDATFIRQSRRAPFIALAESLGVPWRVLAFDAP
                     ERVLRERVRRRQAAGGDASEAGEEVLASQLKNREPLAPHEAAHALLIDTQMAVDWTAW
                     SAALPKG"
BASE COUNT          305 a          792 c          823 g          366 t
ORIGIN      
        1 cgcccaagac cacgctgggc aagctgttca ccgtcggcta cgtggtgtgc gggctgccgc
       61 cgcacaccac gccgcgcgac ggcagcgacc tgctgtgagc catcgtgccc gcgctgctgg
      121 gcgcggccgt cggcatgctg gcccgctgga gccagccgcg cctggcacac gcgctggcgc
      181 gcctgggcaa cccgctggtg cagacgctgg ccggcacggc gctgtttgcc ctgctggcgg
      241 cggtcgtgcc gctggtgcgc ttttcgggcc accacgaact ggtgcacgcg ctggcacacg
      301 gcgtggtcgc cggcgccgcc gcgctgcttg gcctggccgt gctcaaggcg ctggcgatgg
      361 cgctgtgcct ggccagcggc tggcgtggcg gcgagatttt tccgctgctg tttgccggcg
      421 ccgccgctgg cctggccgcc gtgcatgcgc tgcccggcct gccggtgacg gtcgcgctgg
      481 tggccggcat gacggccgcc gccacggtgg gcatgggcaa gccggtggcg gtgctgctca
      541 tcatgctgct gctgaccggc gccagcgcgc ccgggacgct ttgcgtgggc gcgttcgtgg
      601 gctatggcgc ggcgcggctg ttgccggcgc gggcggggca ttgacccaac gcacagcaga
      661 aagttttttg tcaaaagtgc cgccagcgct ttctgatcaa gcgcgagcag ctcttgtatt
      721 gatagcgtta gcccttcggc aaggcggcgc tccacgccgt ccaatccacc gccatttggg
      781 tgtcaatcag cagcgcgtgc gcggcctcgt gcggcgccag cggttcgcgg ttcttcaatt
      841 ggcttgcgag cacttcctcg ccggcttccg acgcatcgcc gcccgccgcc tgccgccggc
      901 gcacgcgttc gcgcagcacg cgttcaggcg cgtcgaaagc caggacgcgc cacggcacgc
      961 ccagcgattc ggccagcgcg atgaagggcg cgcggcgcga ttggcggatg aaggtggcgt
     1021 cgaccaccac cggctgcccc gcctgcagcg cgccgcgcgc cacttcaagc aggcgcgcga
     1081 acacgcggtc gctggtggct tcgctgtaga tgccgccagg cacgcctgcg ctgctcgcct
     1141 ctggcgcgag gccgaacagg cgcttgcgtt ccacgtcggc gcgcacgcgc accagaccgc
     1201 gctgctcgat caggctttgc gtctggctgc tcttgcccga accgctgacg cccagggtga
     1261 gcgccagcca gggctggcgc ggcgcggcca gttctgccgc gagctgcaca tagcgttcga
     1321 cctctgccag cgcggcatcg tcgccctgcg ccacgcagag cgccgccacc ttggcgcgca
     1381 ccagggcgcg gtacacggcg tcgtggggca ggcgctgcac cgcttcgtag tcgccggtct
     1441 gctccagcca ggcgttcaga aagcgccagg ccagcggcgc cagaccgtgc gcgtgcaaat
     1501 ccatgaacag aaaggcgatg tcgcacgcgg tgtcgatcca gcgcaggcgc gggtcgaatt
     1561 cgatggcgtc gaacagcagc gggcgcccat ccagccacag caggttgccc aagtgcagat
     1621 cgccatgacc ttcgcgcacg tgtccggcgg cgcggcgcgc ggccatcagc ggggccaggc
     1681 gcgtgccttc ggttcgtgtc caatgctgca atttcaatag ctgctcgcgc ttgccagaca
     1741 agcgctgcag gcctaaaaga ggcttgaagt tgtcctgcgc cagttcggcc agctgcgcgg
     1801 cgtcgccata gtggctgccc ggcggtgcca cgtcggcgcg cgactgaaag gcggcgatct
     1861 cgcgcgccag cgcgtcgatg tgctcggcct gcaattcgcc gcgcgcggcg cggccttcca
     1921 gcaatgcgtc ggccggaaag cggcgcatgt gcacggccca gtcgatgggt tctccgctgc
     1981 cgtcaagttg cggcgcgtcg ggcgagccgg tgacgggcag cacgcgcatg taaagatcgg
     2041 gcgcggtgcg gcggttgatg cgcagctctt catcgcacgc ggcgcggcgt tgctcaaggc
     2101 ggctgaaatc gaggaagtcg agcttgacgg gcttcttcag cttgtaggcg tcagcgcccg
     2161 acaggagcag ccaggagatg tgggtctcaa ccagctcggt gccggcgccc agcgtgcggc
     2221 gcagggcttt gatcaggggc gcggcagcgg gggtggtcac gtggtcagcc ctcaccccag
     2281 ccctct
//
LOCUS       DAOMVO010000373         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14056_length_2265_cov_21.420814, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000373 DAOMVO010000000
VERSION     DAOMVO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_2265_cov_21.420814"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="tolQ"
                     /locus_tag="PKV30_12970"
     CDS             complement(<1..674)
                     /gene="tolQ"
                     /locus_tag="PKV30_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRL31162.1"
                     /translation="MNQDLSILHLVVNASFIVQLVMLLLLIGSITSWAAIFRKLRALK
                     NIKTQNEEFERDFWSGTSLNDLYAAAAQNAKQGGPMERIFASGMREYQKLRERRISDA
                     ATLLDGARRAMRASFQREMDAAETNLSFLASVGSVSPYVGLFGTVWGIMHAFTGLGAL
                     QQVTLATVAPGIAEALVATALGLFAAIPAVVAYNRFARDIDRIAIKQETFIEEFSNIL
                     QRNLGV"
     gene            complement(671..1084)
                     /gene="ybgC"
                     /locus_tag="PKV30_12975"
     CDS             complement(671..1084)
                     /gene="ybgC"
                     /locus_tag="PKV30_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRL31163.1"
                     /translation="MAFEFPIRIYWEDTDAGGIVFYANYLKFFERARTEWLRALGIHQ
                     RQLREQCGGMFVVADAGLKYLRPARLDDELIVTARLQEAGRAGMVLAQQAFLAGATPA
                     DRTLLCEGRIRAGWVDAQTMKPARIPSDILQKLDP"
     gene            complement(1099..>2265)
                     /locus_tag="PKV30_12980"
     CDS             complement(1099..>2265)
                     /locus_tag="PKV30_12980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRL31164.1"
                     /translation="AAAIAREAAGTDRPMIYLNIGEPDFTAPPLVQEAAEKAIHDGRT
                     QYTQATGLPALRERISDWYRERFGLDVPARRIVVTAGASAALQLACLALIDAGDEVLM
                     PDPSYPCNRQFVSAADGRAVLIPSTAAERFQLSAAKVAQAWGAHTRGVLLASPSNPTG
                     TSIHPDELKRIHDVVFERGGITLLDEIYLGLSFDDTYSHSALGLGDQIISINSFSKYF
                     NMTGWRLGWLVVPESIVDTVEQIAQHLFICPSTVSQYAALACFEKESLAEYERRRAEF
                     KARRDWFVPQLNALGLHVPVTPDGAFYAWADCRAACDKLFGPASAARSEYNSGMQCAG
                     SWDFAFELMKRAHLAVTPGRDFGHADTARFIRFSTANSMAHLQTAVDRLREVLA"
BASE COUNT          385 a          708 c          784 g          388 t
ORIGIN      
        1 tgcacgccca ggttgcgctg caggatgttg gagaactcct cgatgaaggt ctcctgcttg
       61 atggcaatgc ggtcgatgtc gcgcgcgaag cggttgtagg ccaccacggc cgggatggcg
      121 gcgaacagcc ccagcgccgt ggccaccagc gcctcggcaa tgccgggggc cacggtggcc
      181 agcgtcacct gctgcagagc gccgaggccg gtgaaggcgt gcatgatgcc ccacacggtg
      241 ccgaacaggc cgacatacgg cgataccgag ccgaccgagg cgagaaagga gaggttggtt
      301 tcggccgcgt ccatctcgcg ctggaagctg gcgcgcatgg cgcggcgggc gccgtcgagc
      361 agggtggcgg cgtcgctgat gcggcgctcg cgcagcttct ggtattcgcg catgccgctg
      421 gcgaagatgc gctccatcgg gccgccttgc ttggcgttct gcgcggccgc agcgtacagg
      481 tcgttcaggc tggtgcccga ccagaagtcg cgctcgaact cctcgttttg cgtcttgatg
      541 tttttcagcg cgcgcagttt gcgaaagatg gccgcccaac tggtgatgga gccgatcaac
      601 agcagcagca tgaccagctg cacgatgaag ctggcgttga ccaccaggtg gaggatggag
      661 aggtcctggt tcatggatcg agtttttgca ggatgtcgga aggaatgcgc gccggcttca
      721 tcgtctgggc gtcgacccag ccggcgcgga tgcggccctc gcacagcagg gtgcgatccg
      781 cgggcgtggc gcccgccaga aacgcctgtt gcgccagcac catgccggcc cggccggctt
      841 cttggagccg cgcggtgacg attagttcat catccagccg cgccgggcgc aggtacttga
      901 gcccggcgtc ggcgaccacg aacatgccgc cgcactgttc acgcaactgg cgctggtgga
      961 tgcccaatgc acgcagccac tcggtgcggg cgcgttcaaa aaacttcagg tagttggcgt
     1021 agaacacgat gccgccggcg tcggtgtctt cccagtagat gcggatcggg aactcaaacg
     1081 ccatcggatc gccgcccgtc aggccagcac ctcgcgcagg cgatccacgg cggtctgcaa
     1141 atgcgccatt gaattggcgg tcgaaaagcg aatgaagcgc gccgtgtcgg cgtggccgaa
     1201 gtcgcgcccg ggcgtcaccg ccaggtgcgc gcgtttcatc agctcgaagg cgaaatccca
     1261 actgccggcg cattgcatgc cgctattgta ttcggagcgc gccgcgctgg ccgggccaaa
     1321 caatttgtca catgcggcgc gacaatcggc ccaggcgtag aaggcgccat cgggcgtgac
     1381 ggggacatgc agccccagcg cattcaactg cggcacgaac cagtcgcgcc gcgccttgaa
     1441 ttcagcccgg cggcgctcgt attcggccag gctctctttt tcgaagcagg ccagcgccgc
     1501 gtattgcgac accgtgctgg ggcagatgaa caggtgctgc gcgatctgct cgacggtatc
     1561 aacgatgctt tcaggcacca ccagccagcc caagcgccag ccggtcatgt tgaaatattt
     1621 gctgaagctg ttgatgctga tgatctggtc gcccaaaccc agggcgctgt ggctgtaggt
     1681 gtcgtcaaac gacaagccca gatagatttc atccaacagc gtgatgccgc cgcgctcgaa
     1741 caccacgtcg tggatgcgct tcagctcatc ggggtgaatc gacgtgccgg tggggttgga
     1801 gggcgaggcc agcagcacgc cgcgtgtgtg agcgccccag gcttgggcga ccttggccgc
     1861 gctgagttga aagcgctcgg ccgctgtcga cggaatcagc accgcgcgac cgtcggcggc
     1921 gctgacgaat tggcgattgc acgggtagct cgggtcgggc atcagcactt cgtcgcccgc
     1981 gtcgatcagc gccagacagg ccagttgcag cgcggccgat gcgcccgcgg tgacgacgat
     2041 gcggcgcgcc ggcacgtcaa gcccgaaacg ctcgcgatac cagtcgctga tgcgctcgcg
     2101 cagcgcaggc aggccggtgg cttgggtgta ttgcgtgcgg ccgtcgtgaa tcgctttttc
     2161 ggccgcttcc tgtaccagcg gcggcgcggt gaagtcgggc tcgccgatgt tcaggtagat
     2221 catcggccgg tcggtgccgg cggcctcgcg cgcgatggcg gcggc
//
LOCUS       DAOMVO010000374         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14122_length_2255_cov_15.926818, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000374 DAOMVO010000000
VERSION     DAOMVO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14122_length_2255_cov_15.926818"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1250)
                     /gene="argE"
                     /locus_tag="PKV30_12985"
     CDS             complement(99..1250)
                     /gene="argE"
                     /locus_tag="PKV30_12985"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRL31165.1"
                     /translation="MNTLAWLDRLTSIDTTSRNSNLGLIETARDALKGHGLDPWLNLN
                     AEGTKANLFCTLPAQDGATQGGIALSGHTDVVPVDGQKWSSDPFKLTENAGKLYARGA
                     CDMKGFIAASLALVPEFMALPRSKPMHLALSYDEEISCLGAPFMLAELQRRGIRVDGC
                     IVGEPTSMRPVVAHKGLNAYRCRVHGKAAHSSLTTQGCNAIEHAARLICHIRDLAQMW
                     RSEGPFDEFYDVPYTTITTNQIAGGIAMNTIPDACEFTYEFRNLAGVTPQQVQARIGA
                     YVQGELLPRMQREFAGARVEIDKLAAAPGLDASEQAAITQLVRALTGDDSVRKVAYGT
                     EAGLFQGIGIPTVVCGPGSIEQAHKPDEFVEVDQLAQCEKFLRQLAATL"
     gene            1405..>2255
                     /gene="htpX"
                     /locus_tag="PKV30_12990"
     CDS             1405..>2255
                     /gene="htpX"
                     /locus_tag="PKV30_12990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HRL31166.1"
                     /translation="MKRILLFVLTNVAVMAVVGLVSSLFGLGRYVGTNTGQLMIFSLL
                     VGFTGAIVSLLMSKSMAKMTMGLKVINQPANADEAWIVETVRKFADKAGIGMPEVAIY
                     EGEPNAFATGAFKNSALVAVSTGLLRGMTREEVEAVIGHEVAHVANGDMVTMALIQGV
                     MNTFVVFASRMIGSLVDGAMRRGENSGPGIGYYVTTVVLDILFGFLAGIIVAWFSRQR
                     EFRADRGSAQLMGNPRNMINALARLGGLHAEGQQMPKNLQTMAIVGSIGKLFATHPPM
                     EERIAAL"
BASE COUNT          357 a          736 c          764 g          398 t
ORIGIN      
        1 atcaggggcg cggcagcggg ggtggtcacg tggtcagccc tcaccccagc cctctcccag
       61 ggcgagaggg ggcaagaccg gggcgcagcc ggtcacggtt acagcgtggc ggccaactgg
      121 cgcagaaact tctcgcactg cgccagttga tcgacctcca caaactcatc cggcttgtgc
      181 gcctgctcga tcgagcccgg gccgcacacc acggtgggga tgccgatgcc ctggaacagc
      241 ccggcctcgg tgccgtaggc gaccttgcgc acgctgtcgt cgccggtcag cgcgcgcacc
      301 agttgcgtga tggcggcctg ctcgctggcg tcgaggccgg gcgcggcggc cagcttgtcg
      361 atctcgacgc gcgcgccggc aaactcgcgc tgcatgcgcg gcagcagctc gccctgcacg
      421 taggcgccaa tgcgcgcctg cacttgctgc ggcgtcacgc cggccaggtt gcggaattcg
      481 taggtgaact cgcaggcgtc ggggatggtg ttcatcgcga tgccgccggc gatctggttg
      541 gtggtgatgg tggtgtaggg cacgtcgtag aactcatcga acggcccttc gctgcgccac
      601 atctgcgcca ggtcgcggat gtggcagatc agccgcgccg cgtgctcgat ggcgttgcag
      661 ccttgcgtgg tgagtgacga atgcgccgcc ttgccgtgca cgcggcagcg gtaggcgttc
      721 aagcccttgt gcgccaccac cggccgcatc gacgtcggct cgccaacgat gcaaccatcg
      781 acgcggatgc cgcgccgctg cagttcggcc agcatgaagg gcgcgcccag gcatgaaatt
      841 tcctcgtcgt acgacagcgc caggtgcatg ggcttgctgc gcggcagcgc catgaattca
      901 ggcaccagcg ccaggctggc ggcgatgaag cccttcatgt cgcaggcgcc gcgcgcgtac
      961 agcttgccgg cattttcggt cagcttgaag gggtcgctcg accacttttg cccatccacc
     1021 ggcaccacgt cggtgtggcc cgacagcgcg atgccgccct gggtggcacc gtcttgcgcg
     1081 ggcagggtgc agaacaggtt ggccttggtg ccttcggcgt tcaggttcag ccacggatcg
     1141 aggccgtggc ccttgagcgc gtcgcgcgca gtctcgatca ggccgagatt cgagttgcgg
     1201 ctggtggtgt cgatggacgt caggcggtcg agccaggcga gggtgttcat gcagcggatg
     1261 ctccttgcgt acagtgtagg acgctggcca aagtcgcgcc tgcggggcgc ccggtgtggg
     1321 aacccgccgt attgaatctg ctccaatcgc ccctaccatt gcacatcgcg cccgcagccg
     1381 ctggcgccca ccaagaggga acccatgaaa cgcatcctgc tctttgtcct gaccaacgtc
     1441 gccgtgatgg cggtggtggg cctggtcagc agcctgtttg gcctgggccg ctatgtcggc
     1501 accaacaccg ggcagttgat gattttttcg ctgctggtcg gcttcaccgg cgccatcgtg
     1561 tcgctcttga tgagcaagtc catggccaag atgaccatgg gcctgaaggt catcaaccag
     1621 ccggccaacg ccgacgaagc ctggatcgtc gagacggtgc gcaagtttgc cgacaaggcc
     1681 ggcatcggca tgccggaagt ggcgatttac gagggcgagc ccaacgcctt tgccaccggc
     1741 gcgttcaaga attcggcgct ggtggccgtc tccaccggcc tgttgcgcgg catgacgcgc
     1801 gaggaggtcg aggccgtcat cggccacgag gtggcgcacg tggccaacgg cgacatggtg
     1861 accatggcgc tgatccaggg cgtgatgaac accttcgtcg tgtttgccag ccgcatgatc
     1921 ggctcgctgg tcgatggcgc catgcgccgt ggcgaaaatt ctggccccgg catcggctac
     1981 tacgtgacca ccgtggtgct cgacattttg ttcggctttc tggccggcat catcgtcgcc
     2041 tggttctcgc gccagcgcga gttccgcgcc gaccgcggct cggcccaact catgggcaac
     2101 ccgcgcaaca tgatcaacgc cctggcgcgc ctgggcggcc tgcacgccga ggggcagcaa
     2161 atgcccaaga acctgcaaac catggccatt gtcggcagca tcggcaagct gttcgccacc
     2221 cacccgccga tggaagagcg cattgcggcg ctgaa
//
LOCUS       DAOMVO010000375         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14185_length_2249_cov_12.052871, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000375 DAOMVO010000000
VERSION     DAOMVO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14185_length_2249_cov_12.052871"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKV30_12995"
     CDS             complement(<1..976)
                     /locus_tag="PKV30_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRL31167.1"
                     /translation="MAASVDICIRGAGIVGRTLALLLARERLQVALVAPPPDAASGHG
                     DVRAYALSPTARTLLQGLRCWPNERAATPVLAMEVHGDERGAVRFDATAQGVPALNWI
                     VDVPALEKLLAEAVNYQSGIDVVAEPVAAPLTVVCEGRASRTRAELGVEFDTTRYPQH
                     AIATRLDCERPHGQVARQWFTAEGDIVAFLPLDGADGRQVAVVWSVLAERVPALLALD
                     DAAFAARLYELSGGALGALAQTGPRASWPLLLARATRWVGPLPGCPGESFALAGDAAH
                     AMHPLAGQGLNVGLGDAAELAQVLAAREPWRSPGDLKLLRRYERARQGD"
     gene            1074..1964
                     /locus_tag="PKV30_13000"
     CDS             1074..1964
                     /locus_tag="PKV30_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HRL31168.1"
                     /translation="MDIVVTNPPADADVRATVSALFVHPVKSCAGVALSEAQLMDTGL
                     DLDRAWMVVDAAGRFVTQRELPRMALVRPQIRTLEVVLRAPGMLALHLGLNEVEKPTR
                     VQVWKDEVAAWDMGDVAAQWFSDFLGVPGLRLARFDPEVTRLASKHWTGEIDAPVEFA
                     DGFPLLLASQASLDELNARLAAAGHAPVGMERFRPNIVIDGVAAQDEDRLAELRITTG
                     EGDTVVLRPVKPCPRCPIPNVDPATGVSSPEVLDQLATYRAHPVLDGAITFGMNCVIV
                     QGAGATLRAGDAVSADWRFD"
     gene            2101..>2249
                     /locus_tag="PKV30_13005"
     CDS             2101..>2249
                     /locus_tag="PKV30_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRL31169.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNTGVV
                     EVPDPR"
BASE COUNT          343 a          805 c          773 g          328 t
ORIGIN      
        1 agtcgccctg gcgcgcgcgc tcgtagcggc gcagcagctt caaatcgccc ggcgaacgcc
       61 acggctcgcg cgcggccagc acctgcgcca gctcggcggc atcgcccagg ccgacgttca
      121 ggccctggcc ggccagcggg tgcatggcgt gggcggcgtc gccggccagc gcaaagctct
      181 cgcccgggca gccaggcagg gggccgaccc agcgcgtggc gcgcgccagc agcagcggcc
      241 agctggcgcg cgggccggtt tgcgccagcg cgcccaaagc gccgccgctc aattcataca
      301 ggcgcgcggc aaaggccgcg tcgtcgagcg ccagcagcgc gggtacgcgc tcggccagca
      361 ccgaccagac gacggccacc tgacggccgt cggcgccgtc cagcggcaga aaggcgacga
      421 tgtcgccctc ggccgtgaac cactggcgcg ccacctggcc gtgcgggcgc tcgcaatcga
      481 gccgcgtggc aatggcgtgc tgcgggtagc gcgtggtgtc gaactccacg cccagctcgg
      541 cgcgcgtgcg gctggcgcgg ccttcgcaca ccacggtgag cggcgccgcc acgggctcgg
      601 ccaccacgtc gatgccgctt tggtaattga cggcttcggc cagcagcttt tcgagcgctg
      661 gcacgtcgac gatccagttc agcgccggca cgccttgcgc cgtggcgtca aatcgcaccg
      721 cgccgcgctc gtcgccgtgc acttccatcg ccagcacggg cgtggccgcg cgctcgttcg
      781 gccagcagcg caggccttgc agcagcgtgc gggcggtggg gctgagggcg taggcgcgca
      841 cgtcgccgtg gccgctggcg gcgtcgggcg gtggcgccac cagcgccacc tgcaaacgct
      901 cgcgcgccag cagcagcgcc agcgtgcggc ccacaatgcc ggcgccacgg atgcaaatgt
      961 cgactgaagc ggccatgatg ggggcattgt aggcggcgcg gttcagtggg gaggtggcac
     1021 cgttttgtga tcaaatccgg cctccggcgc ttgtccagca agcgcgacca gctatggata
     1081 tcgtagtgac caatccccct gccgatgccg atgtgcgcgc cacagtgagc gcgcttttcg
     1141 ttcaccccgt gaagtcctgc gcaggcgtgg cgctgtctga ggcgcagttg atggacaccg
     1201 ggctcgacct ggaccgcgcc tggatggtgg tcgacgccgc cggccgcttc gtcacccagc
     1261 gcgagctgcc gcgcatggcg ctggtgcgcc cgcagatccg cacgctggaa gtcgtgctgc
     1321 gcgcgcccgg catgctggcg ctgcacttgg ggctaaacga ggtcgaaaaa cccacccgcg
     1381 tgcaggtctg gaaggacgaa gtcgccgcct gggacatggg cgacgtggct gcgcagtggt
     1441 tcagcgactt tctgggcgtg cccggcctgc gcctggcgcg ctttgacccc gaagtgacgc
     1501 gcttggcatc caaacattgg acgggcgaaa tcgacgcgcc ggtcgagttt gccgacggct
     1561 ttccgctgct gctggccagc caggcctcgc tggatgaact caacgcgcgc ctggccgccg
     1621 ccggccacgc gcccgtgggc atggagcgct ttcgccccaa catcgtcatc gacggcgtgg
     1681 ccgcgcagga cgaagaccgg ctggccgagc tgcgcatcac caccggcgag ggcgacaccg
     1741 tggtgctgcg ccccgtcaag ccctgcccgc gctgccccat ccccaacgtc gaccccgcca
     1801 ccggcgtgag cagccccgaa gtgctggacc agctcgccac ctaccgcgcc cacccggtgc
     1861 tggacggcgc catcaccttc ggcatgaact gcgtcatcgt gcaaggcgcg ggcgccacgc
     1921 tgcgcgcggg cgatgcggtc agcgccgatt ggcgatttga ttgacgctat ttttatggta
     1981 gctgcccgcg cttgacagac aagcgccaga gcccaatttg attcataaac ccgtggcccc
     2041 gaacgtattg gcgcgccggt agaatcagcg gtttccctcg attccaacga aagccccgac
     2101 atgagcctgc aatgcggcat cgtgggcctg cccaacgtgg gcaagtccac gcttttcaac
     2161 gcgctgacca aagccggcat cgcggccgag aactacccct tttgcaccat cgagccgaac
     2221 accggcgtgg tcgaggtgcc cgatccgcg
//
LOCUS       DAOMVO010000376         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14193_length_2248_cov_12.866849, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000376 DAOMVO010000000
VERSION     DAOMVO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14193_length_2248_cov_12.866849"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKV30_13010"
     CDS             <1..1205
                     /locus_tag="PKV30_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL31170.1"
                     /translation="KFNQLAGINAFPVTPPSLGQGFRERPVNFVVQTSDSYESLNAVM
                     RKLMDEVARNPGFVAPDVDLRLSKPELRIEVDRERAADMGVSVEAVARAIETMLGGRQ
                     VTRYKRDAEQYDVIVQTAVTGRSTPEDIDRIQLRASKNGVDAMVPLSALVKVHESVSP
                     RELNHFGQRRSATLTSNLAPNYSLGEALAFLDTTANQVLKGGYTTDLNGISREFRSSQ
                     GALVIVFVLALLFIFLVLAAQFESFVDPLIIMFSVPLSMIGALLALQLTGGSLNVYSQ
                     IGLITLVGLVTKHGILIVEFTNQLRQRGEPMLDALVHASAQRLRPILMTTGAMVLGAL
                     PLALAHGAGAESRQQIGWVIVGGMSVGTLLTIFVVPTMYALLARQRVPGAKTTPVEAP
                     HAEPAHGA"
     gene            1287..>2248
                     /locus_tag="PKV30_13015"
     CDS             1287..>2248
                     /locus_tag="PKV30_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31171.1"
                     /translation="MTPAEFIAKWGPGGPAHGLNEEQGAQSHFLDLCELLDVPKPGSA
                     DDYLFERRSLQLGEARGYADVFYRDHFAWENKAPGKNLDAALRQLQQYSLALANPPLL
                     VVCDRLVIRIHTQFNGHPSETHTVRLDQLDQPGARELLRRLWTAPESFRPRATNRDIT
                     EAAAKSFATLAEQLRARGPTEADARRAHADTVAHFLTQCLFCFFAEDVGLLPGRMFER
                     LVSNRQLTAERLTAGLAQLFTTMQTGGLYGPDDIPWFNGGLFARIDVPALQIVDITEL
                     RNAAALNWSAIDVSIFGTLFERGLDPAKRSQLGAHYTDPATINR"
BASE COUNT          370 a          832 c          695 g          351 t
ORIGIN      
        1 ccaagttcaa ccagctggcg ggcatcaacg cctttccggt cacgccgccg tcgctcggcc
       61 agggctttcg cgagcggccg gtcaacttcg tggtgcaaac gtccgacagc tacgaaagcc
      121 tgaacgccgt catgcgcaag ctgatggacg aagtggccag gaaccccggc ttcgtggcgc
      181 ccgacgtgga tttgcgactg tcgaaaccag agctgcgcat cgaggtcgac cgcgagcgcg
      241 cggccgacat gggcgtcagc gtggaagccg tggcgcgcgc catcgagacc atgctgggtg
      301 gccgccaggt cacccgctac aagcgcgacg ccgagcagta cgacgtcatc gtgcagaccg
      361 cggtcaccgg ccgcagcacg cccgaggaca tcgaccgcat ccagctgcgc gccagcaaga
      421 acggcgtcga cgccatggtg ccgctgtcgg cgctggtcaa ggtgcatgaa agcgtgtcgc
      481 cgcgcgagct gaaccacttc ggccagcgcc gctcggccac gctgaccagc aacctggcgc
      541 ccaattattc gctgggcgag gcgctggcct ttctcgacac caccgccaac caggtgctga
      601 aaggcggcta caccaccgac ctgaacggca tctcgcgcga attccgctcc agccagggcg
      661 cgctggtcat cgtgttcgtg ctggcgctgc tgttcatctt tctggtgctg gcggcgcagt
      721 tcgagagctt tgtcgacccg ctcatcatca tgttctcggt gccgctgtcg atgattggcg
      781 cgctgctggc gctgcagctc acgggcggct cgctcaacgt ctattcgcag atcggcctca
      841 tcacgctggt ggggctggtc accaagcacg gcatcctgat cgtcgagttc accaaccagc
      901 tgcgccagcg cggcgagccg atgctggatg cgctggtgca cgccagcgcg cagcgcctgc
      961 gccccatctt gatgaccacc ggcgccatgg tgctgggcgc cttgccgctg gcgctggccc
     1021 acggcgccgg cgccgaaagc cgccagcaga tcggctgggt catcgtcggc gggatgagcg
     1081 tgggcacgct gctcaccatc ttcgtcgtgc cgaccatgta cgcactgctg gcgcgccagc
     1141 gcgtgccggg cgccaagacc acgccggtcg aggcgccgca cgccgagccc gcgcacggtg
     1201 cttgagccgg tatgaaaaca atagctgcct gcgcttgcca ggcaagcgct gaaggcctat
     1261 ttcgcttatg aatccgtgtt ccccgcatga cgcccgctga attcatcgcc aaatggggcc
     1321 cgggcggccc ggcccatggg ctcaacgaag agcagggcgc gcaaagccac tttctcgacc
     1381 tgtgcgaatt gctcgatgtg cccaagcccg gcagcgccga cgactacctg ttcgagcgcc
     1441 gctcgctgca gctgggcgag gcgcgtggtt atgccgacgt gttctaccgc gaccacttcg
     1501 cgtgggagaa caaggccccc ggcaagaacc tcgacgccgc cctgcgccag ctgcagcaat
     1561 acagcctggc gctggccaac ccgccgctgc tggtggtgtg cgaccgcctc gtcatccgca
     1621 tccacaccca gttcaacggc cacccgtccg agacccacac cgtccggctc gaccagcttg
     1681 accagccggg cgcgcgcgag ctgctgcgcc gcctgtggac cgcgccggag agctttcgcc
     1741 cccgcgccac caaccgcgac atcaccgaag ccgccgccaa aagctttgcc accctggccg
     1801 agcaactgcg cgcgcgcggc cccaccgagg ccgacgcgcg ccgtgcccac gccgacaccg
     1861 tggcgcactt tctcacccag tgcctgttct gcttttttgc cgaagacgtg ggcctgctgc
     1921 ccggccgcat gttcgagcgg ctggtctcca accgccagct caccgccgag cgcctgaccg
     1981 ccggcctggc gcaactgttc accaccatgc agaccggcgg cctgtacggc cccgacgaca
     2041 tcccctggtt caacggcggc ctgtttgccc gcatcgacgt gcccgcgctg caaatcgtcg
     2101 acatcaccga gctgcgcaac gccgccgcgc tcaactggag cgccatcgac gtcagcattt
     2161 tcggcaccct gttcgagcgc gggctcgacc ccgccaagcg cagccagctc ggcgcccact
     2221 acaccgaccc ggccaccatc aaccgcat
//
LOCUS       DAOMVO010000377         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14233_length_2243_cov_11.968921, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000377 DAOMVO010000000
VERSION     DAOMVO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14233_length_2243_cov_11.968921"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKV30_13020"
     CDS             <1..421
                     /locus_tag="PKV30_13020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRL31172.1"
                     /translation="AFAERRVRKHYQALVHGRAVLPPGADSVWGEIDLPLLIDWPNRP
                     RSAVNHAAGKPSRTRWRPLAYDAANDITRVALQPLTGRSHQLRVHLQAIGHPIVGDAL
                     YGPAGDGAARLMLHAGGLELAHPTSGQPLVLDSAVPF"
     gene            461..1093
                     /locus_tag="PKV30_13025"
     CDS             461..1093
                     /locus_tag="PKV30_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HRL31173.1"
                     /translation="MHAPHVDPLNRRRATAALVALPWLWTGARAQPAVRHRLTPAQTE
                     GPFYPVALPADSDTDLLRNAARPYAQGTPARVEGRVLDVDGQPVKGAQIEIWQCDQQG
                     HYHHPRDGGRADPAFQGFGRTTVAADGAWRFRTIRPAPYTGRTPHIHVKVRLGTRELL
                     TTQLYVEGDPGNARDFLWRSLDEHGRRALTQAFSASADGLQAAYTLIVAA"
     gene            1219..1803
                     /locus_tag="PKV30_13030"
     CDS             1219..1803
                     /locus_tag="PKV30_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HRL31174.1"
                     /translation="MKKMNLRSIGLAVALGLGSIAMVGCTTTPKGAEASGPTARAAID
                     RDVDATLSRLYSTVPGTREMVQRAAGVLVFPSVVGGSFVVGAEYGKGALREGGITTGY
                     YSMTAGSIGFQAGGQSKAVIYVFNTGEALQKFKASNGWTAGADATVAVGKIGANGSVD
                     TETMKQAVSSFILTNVGLEAGASVGAAKVTRISL"
     gene            1911..>2243
                     /locus_tag="PKV30_13035"
     CDS             1911..>2243
                     /locus_tag="PKV30_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL31175.1"
                     /translation="MTDPDAHLFILTGASRGMGLAMARQLLVPGHTVLTLSRLPSDDL
                     ARHAESAGAGLTQWSVDLADSAPVAERLSAWLGEFDVGQFASANLINNAGVIPAIVPL
                     RDAAPADLA"
BASE COUNT          347 a          808 c          759 g          329 t
ORIGIN      
        1 cgcctttgcc gagcgccgcg tgcgcaagca ctaccaggcg ctggtgcacg gccgcgcagt
       61 tttgccgccc ggcgccgatt cggtatgggg cgagatcgac ctgcccctgc tgatcgactg
      121 gccgaaccgc ccgcgcagcg cggtcaacca cgctgccggc aagcccagcc gcacgcgctg
      181 gcgcccgctg gcgtacgacg ctgccaacga catcacccgg gtcgcgctgc aacctttgac
      241 cggccgctcg caccagctgc gcgtgcacct gcaggccatt ggccacccca tcgtcggcga
      301 cgcgctgtac gggcccgccg gtgacggcgc ggcgcgcctg atgctgcacg caggcgggct
      361 tgaactcgcg cacccgacca gcgggcagcc tttggtgctc gacagcgcgg tgccgttcta
      421 ggcgccggaa cttcgcgccg gcagcgcgcc tcccacggcc atgcacgcac cgcacgtcga
      481 cccgctcaac cgccgccgcg ccacggccgc gctggtggcc ctgccttggc tttggacggg
      541 cgcgcgggcg cagccggcgg tgcgccatcg gctcacgccg gcgcagaccg aggggccgtt
      601 ctaccctgtg gcgctgccgg ccgacagcga cacggatctg ctgcgcaacg ccgcgcggcc
      661 ctacgcgcag ggcacgccgg cgcgcgtcga aggccgtgtg ctggacgtcg atggccagcc
      721 ggtgaagggc gcgcagatcg aaatctggca gtgcgaccag cagggccact accaccaccc
      781 gcgcgacggc gggcgcgccg acccggcgtt ccagggtttc ggccgcacga ccgtggctgc
      841 cgatggcgcc tggcggtttc gcaccatccg gccggcgccc tatacggggc gcacaccgca
      901 catccacgtc aaggtgcggc tgggcacgcg cgaactgctc accacgcagt tgtacgtcga
      961 gggcgacccg ggcaacgcgc gcgattttct ctggcgcagc ctggatgagc acgggcgtcg
     1021 cgcgctgacg caagccttca gcgccagcgc ggatggcttg caggccgcgt acacgctcat
     1081 cgttgcggcc tgaaaaaaac gcgcgcgcag cctgcggcgt ggcgccacag cggggcgggt
     1141 tgccgtccta cgcaggccgc acacgccggc ttgtaaagtc cttacacctt tcgatcaact
     1201 ccaacgagga cattcaccat gaaaaaaatg aacctgcgca gcatcggtct ggccgtcgcg
     1261 ctcggcttgg gcagcatcgc catggtcggc tgcacgacca cacccaaggg tgccgaggca
     1321 tcaggcccca cggcccgtgc cgcgatcgat cgtgacgtgg acgccacgct ttcgcgcctg
     1381 tacagcacag tgcctggcac gcgtgaaatg gtgcagcgcg cggccggtgt gctggtgttc
     1441 ccgtcggtgg tcggcggcag cttcgtggtc ggtgccgaat acggcaaggg cgcactgcgc
     1501 gaaggcggca tcaccaccgg ttattactcg atgacggcag gctcgatcgg cttccaggcc
     1561 ggtggccagt cgaaggcagt gatctacgtg ttcaacaccg gcgaggcgct gcagaagttc
     1621 aaggccagca acggctggac ggccggcgcc gatgccaccg tggcggtcgg caagatcggc
     1681 gccaacggct cggtcgacac cgaaaccatg aagcaggccg tgagcagctt catcctgacc
     1741 aatgtgggcc tggaagcagg tgcctcggtg ggcgccgcca aggtgacgcg catcagcctg
     1801 taagggcatc gggcatgtcg gccacgcgcc gcaagcccga attcatttga tggacgccgc
     1861 gcactgtcgc ggcgtttttc ttgggcgcca gatgcgctaa attgcctggc atgaccgatc
     1921 ccgacgccca cctcttcatc ctcaccggcg cctcgcgcgg catgggcttg gccatggcgc
     1981 gccagttgct cgtgcccggc cacaccgtgc tgaccttgtc gcgcctgccc agcgacgatc
     2041 tggcacggca cgctgaatcg gccggcgccg ggttgacgca atggtcggtc gacctggccg
     2101 acagcgcgcc cgtggccgag cggctgtcag cgtggctggg cgagttcgac gtcggccaat
     2161 tcgccagcgc caacctgatc aacaacgccg gcgtcatccc cgccatcgtg ccgctgcgcg
     2221 acgccgcgcc ggccgatctg gcc
//
LOCUS       DAOMVO010000378         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14336_length_2231_cov_14.537224, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000378 DAOMVO010000000
VERSION     DAOMVO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14336_length_2231_cov_14.537224"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKV30_13040"
     CDS             <1..569
                     /locus_tag="PKV30_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HRL31176.1"
                     /translation="EGITGGDDYAAMRQVLERRYGKIAEALREQGSEAVTAAARMPDL
                     VLVDGGKGQVSMARAVFEQLGLDVSLIVGVEKGEGRKVGLEELVFADGREKVYLGRDS
                     AALMLVAQIRDEAHRFAITGMRAQRAKVRVGGSRIEEIPGVGPRKRARLLQRFGGVRG
                     VEAASVADLMSVDGISRALAEEIYRALH"
     gene            700..1266
                     /gene="pgsA"
                     /locus_tag="PKV30_13045"
     CDS             700..1266
                     /gene="pgsA"
                     /locus_tag="PKV30_13045"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRL31177.1"
                     /translation="MFFTLPTLLTWTRIVAIPLIVGVFYLPIEQQTANMTATVMFIVF
                     AWTDWLDGFLARRLNQTSSFGAFLDPVADKFLVCASLLVLVHLQRTDVFVALIIIGRE
                     IAISALREWMAKIGASKSVAVHMLGKVKTTVQMVAIPFLLYDARLFDVIDTRWWGQVL
                     IWVSAVLTVWSMVYYLQKALPEIRARVE"
     gene            1418..>2231
                     /locus_tag="PKV30_13050"
     CDS             1418..>2231
                     /locus_tag="PKV30_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL31178.1"
                     /translation="MTTDDGRALLSRWMPAVFVLIWATGFIVARYGMPYAPPFTFLLY
                     RYLLSILCFGVWIAIARVRWPQTGIEWLHLGMTGVLMHAGYLGGVWAAVKGGMGSGLS
                     SLIVGIQPVLTAIWLSAIGGAASRVSGRQWLGLLLGFAGLVLVVWRKLTQGQAGDHVT
                     AANLSWAVFALFCITAGTLYQKRFVKPCDVRTANTVQLLAAALVTLPLALLEPEAFQW
                     NLQSAGAMAWSVLGLTLGGSSLLYMLIQRGAAASVTSLMYLVPPTTAVMAWLL"
BASE COUNT          313 a          644 c          824 g          450 t
ORIGIN      
        1 tcgagggcat caccggcggc gacgactacg ccgccatgcg ccaggtgctg gagcggcgct
       61 acggcaagat cgccgaggcg ctgcgcgagc agggcagcga ggcggtgacg gcggccgcgc
      121 gcatgcccga tctggtgctg gtcgatggcg gcaagggcca ggtcagcatg gcgcgcgccg
      181 tgttcgagca gctggggctg gacgtgtcgc tgatcgtcgg cgtcgagaag ggcgagggcc
      241 gcaaggtcgg gctggaagaa ctggtgtttg ccgacgggcg cgagaaggtt tatctgggcc
      301 gcgattcggc ggcgctgatg ctggtggcgc aaatccgcga cgaggcgcac cgctttgcca
      361 tcaccggcat gcgcgcgcaa cgcgccaagg tgcgcgtggg cggcagccgc atcgaggaga
      421 tccccggtgt cggcccgcgc aagcgcgccc gcctgctgca gcgctttggc ggcgtgcgcg
      481 gcgtggaagc ggccagcgtg gcggacctga tgagcgtgga tggcatttca cgcgcgctgg
      541 ccgaggagat ctaccgcgcg ctgcattgag gcagtgtttc aaatgaaaat ggcctctggc
      601 gcttgatgga caagcgcgag cagctatcaa aaatgtagtt ttggttgttt gctgatggcg
      661 cgtcggaatg gcctgaatcc gcatgacaca atcgccgcca tgttcttcac cctgcccacg
      721 ctgctgacct ggacgcgcat cgtcgcgatt ccgctcatcg tgggggtgtt ctacctgccg
      781 atcgagcagc aaacggccaa catgacggcc acggtgatgt tcatcgtctt tgcgtggacg
      841 gattggctgg acggttttct agcccggcgg ctgaaccaga cctcgtcctt cggcgccttt
      901 ctggacccgg tggcggacaa gttcctggtc tgcgcctcgc tgctcgtatt ggtgcacctg
      961 cagcggaccg atgtgtttgt ggcgctgatc atcatcgggc gcgagatcgc gatttcggcg
     1021 ctgcgcgagt ggatggccaa gatcggcgcc agcaaaagcg tggcggtgca catgctgggc
     1081 aaggtcaaga ccacggtgca gatggtggcc attccgtttc tgctgtacga cgcgcggctg
     1141 ttcgatgtga tcgatacgcg ctggtggggt caagtgctga tctgggtgtc ggcggtgctg
     1201 accgtgtggt cgatggtgta ttacctgcag aaggcgctgc ccgaaatccg cgcacgcgtg
     1261 gaatgacatc ccccctgagc cgcctgcggc gtcttcgccc cagggggaga acgctggtgg
     1321 ccgggccaag cccgttccac ggcgttcccg aatggcctgc tccgcggcca tctggaggcg
     1381 gggttgctgc gcagcccttt ctttttgagt tattggtatg acgactgacg acggccgggc
     1441 gctgttgagt cgctggatgc cggcggtgtt cgtgctgatc tgggccaccg gttttatcgt
     1501 ggcgcgctat ggcatgccgt atgcgccacc gttcacgttt ttgctctacc gctacctgct
     1561 gtcgattctg tgcttcgggg tgtggatcgc cattgcgcgc gtgcgctggc cgcaaaccgg
     1621 catcgagtgg ctgcacctgg gcatgaccgg cgtgttgatg cacgcgggct atctgggcgg
     1681 cgtgtgggcg gcggtcaagg gcggcatggg gtcggggctg tcgtcgctga ttgttggcat
     1741 tcagccagtg ctgacggcga tctggctgtc ggccatcggc ggggcggcca gtcgggtcag
     1801 cgggcggcag tggctggggc tgctgctggg ctttgccggg ctggtgctgg tggtgtggcg
     1861 caagctgacg caagggcagg cgggcgatca tgtgacggcg gcgaatctgt cgtgggcggt
     1921 gttcgcgctg ttttgcatca cggccggcac gctgtatcaa aagcgctttg tcaagccttg
     1981 tgacgtgcgc accgccaaca ccgtgcagtt gctggccgcc gcgctggtga cgctgccgct
     2041 ggcgctgctc gaacccgagg cgttccagtg gaacctgcag tcggccggcg ccatggcgtg
     2101 gtcggtgctg gggctgacgc tgggcggcag ctcgctgttg tatatgctca ttcagcgcgg
     2161 cgccgcggcc tcggtgacca gcctgatgta cctggtgccg cccaccacgg cggtgatggc
     2221 ctggctgctg t
//
LOCUS       DAOMVO010000379         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14367_length_2227_cov_13.108656, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000379 DAOMVO010000000
VERSION     DAOMVO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14367_length_2227_cov_13.108656"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PKV30_13055"
     CDS             complement(<1..1264)
                     /locus_tag="PKV30_13055"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803432.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HRL31179.1"
                     /translation="MLNAYRAHVAERATLGIPPLPLDAKQTAELIELIKNPPAGEEAF
                     LVDLLATRVPPGVDDAAKVKASFLAAVAHGDIAVKLIDKAKATELLGTMVGGYNVKPL
                     IDLLDDKDVASVAAAALKKTLLMFDFFHDVAEKAKAGNAYAKDVIQSWADAEWFTSRP
                     AVPQKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNKRDGAAFVPEEDGK
                     RGPMQFIENLKKKGHLVAYVGDVVGTGSSRKSATNSVIWATGEDIPFVPNKRFGGVTL
                     GGKIAPIFFNTQEDSGALPIEVDVSKFEMGDVLDIYPYDGKIEKGGQTAATFQLKSDV
                     LLDEVQAGGRINLIIGRSLTAKAREFLGLPASDKFRLPQAPAASKAGFTLAQKMVGRA
                     CGLPEGQGVRPGTYCEPKMTTVGSQDTTG"
     gene            complement(1357..1911)
                     /locus_tag="PKV30_13060"
     CDS             complement(1357..1911)
                     /locus_tag="PKV30_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31180.1"
                     /translation="MKLLVIACIAAALGLSTGAVQAQQTKKAPAKSSAKAPTKKTTTA
                     KKRVPAKTAKAVEAHTPVESLSQRLTPAELAIAQTIHAGTISCELGANVTVTPDEKNP
                     GFFRISSGKQSYYMHPVESRTGAIRLEDNRAGALWLQLGNKSMLMNQRLGQRIADECA
                     APQQREFAARMKDQPQVDLLGGSK"
     gene            complement(1967..>2227)
                     /locus_tag="PKV30_13065"
     CDS             complement(1967..>2227)
                     /locus_tag="PKV30_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRL31181.1"
                     /translation="AREFGYTRMWSIHPSQIRPIIEAFSPDEQQIHVASDILLAAAEA
                     DWAPISHQSTLHDRASYRHFWQVLERAHATGRPLPAEVAPWF"
BASE COUNT          348 a          682 c          758 g          439 t
ORIGIN      
        1 gaccggtggt gtcctggctg cccacggtgg tcatcttggg ctcgcagtag gtgccaggtc
       61 gaacgccctg cccctcaggc aaaccgcagg cgcggccgac catcttctgc gccagcgtga
      121 aaccggcctt ggaggcggcg ggcgcctgcg gcaggcggaa cttgtccgac gccggcaggc
      181 ccaggaattc gcgcgccttc gcggtgagcg aacggccaat gatcaggttg atgcggccgc
      241 cagcctgcac ttcgtccagc agcacgtcgc tcttgagctg gaaagtggcc gccgtctggc
      301 cgcccttctc gatcttgccg tcgtaggggt aaatgtcgag cacgtcgccc atttcgaact
      361 tgctcacatc gacctcgatc ggcagcgcac ccgaatcctc ttgcgtgttg aagaagatcg
      421 gcgcgatctt gccgcccagc gtgacgccgc caaagcgctt gttgggcacg aagggaatgt
      481 cctcgcctgt ggcccagatg acggagttgg tggccgactt gcggctggag ccggtgccga
      541 ccacatcgcc cacgtaagcg accaggtggc cctttttctt gaggttctcg atgaactgca
      601 tcgggccgcg cttgccgtct tcctcgggca cgaaagccgc gccgtcgcgc ttgttcttca
      661 gcatggccag gtagtgcagc ggaatgtcgg ggcggctcca ggcgtcgggc gcgggcgaca
      721 aatcgtcggt gttggtctcg cccggcacct tgaacacggt gacggtgatc ttctggggca
      781 ctgcggggcg gctggtgaac cactcggcgt cagcccagct ctggatcacg tccttggcgt
      841 aggcgttgcc ggccttcgct ttttcggcca cgtcgtggaa gaagtcgaac atcagcagcg
      901 tctttttcag cgcggcggcg gcgaccgagg ccacgtcttt atcgtccagc aaatcgatca
      961 gcggcttgac gttgtagccg ccgaccatgg tgccgagtag ctcggtggcc ttggccttgt
     1021 cgatcagctt gacggcgatg tcaccgtgcg cgacggcggc cagaaagctg gccttgacct
     1081 tggccgcatc gtccacgccg ggcggaactc gggtcgcgag caagtcgacc aggaaggcct
     1141 cttcgcccgc gggcgggttc ttgatcagct cgatcagctc ggcagtttgc ttggcatcca
     1201 gcggcagcgg cggaatgccg agggtggcgc gttcggcaac gtgggcgcgg taggcgttca
     1261 acattttctt ctccagatca aaaatcaaac ttcaaacaaa aaaccggcgc cagcgcagac
     1321 ggaagaagcg ctaccagcta ctgaatcagt agcatgtcac ttgctgccgc cgagcaggtc
     1381 tacttgcggc tggtccttca tgcgggcggc aaactcgcgc tgctgcggcg cggcgcattc
     1441 gtcggcgatg cgctggccca gcctctggtt catcagcatc gacttgttgc ccagctgcag
     1501 ccacagcgcg ccggcgcggt tgtcttccag gcggatggcg ccggtgcggc tctccaccgg
     1561 gtgcatgtag tagctctgct tgcccgagct gatgcggaag aagcccgggt tcttttcgtc
     1621 gggcgtgacg gtcacgttgg cacccagttc gcacgaaatg gtgccggcgt gaatggtctg
     1681 cgcgatggcc agctcggccg gcgtcaggcg ctgcgacagc gattcgaccg gtgtgtgcgc
     1741 ctcgacggct tttgcggtct ttgccggcac gcgcttctta gcggtggtgg tcttcttggt
     1801 cggcgccttg gcgctgctct tcgcaggcgc cttcttggtc tgctgagcct gcacagcccc
     1861 cgtggacagg cccagagcgg cggcgatgca tgcgatgacg aggagtttca tactgcaatg
     1921 ggtttcgaaa agtgataagg gcggcaatga tgacatgacg actgggtcag aaccatggcg
     1981 ccacctcggc cggcagcggc cggccggtgg cgtgcgcacg ctcgagcacc tgccagaagt
     2041 ggcggtagct ggcgcggtcg tgcagcgtgc tctggtggct gatgggcgcc cagtcggcct
     2101 cggcagcggc cagcaggatg tcgctggcga catggatctg ctgttcgtcg ggcgagaagg
     2161 cttcgatgat cgggcgaatc tggctcgggt ggatgctcca catgcgggtg tagccgaact
     2221 cgcgcgc
//
LOCUS       DAOMVO010000380         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14402_length_2223_cov_8.851937, whole genome shotgun sequence.
ACCESSION   DAOMVO010000380 DAOMVO010000000
VERSION     DAOMVO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14402_length_2223_cov_8.851937"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PKV30_13070"
     CDS             <1..1597
                     /locus_tag="PKV30_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRL31182.1"
                     /translation="VEVKDTLLSDFYARLIIHPDGRINLQDVIKSKDGAPAVSPAAAP
                     AAARTAPGMPAAGENNGEASNSIAASASQTSASAPKPQQLDPLAPVIRFGPIRMASGH
                     VLFSDRFIRPNYSADLTELNGSLSASSSVAPAGAPQMADLNLTGRAEGTAALQVGGKL
                     NPLAKPLALDIQAKVTDLELPPLSPYSVKYAGHGIERGKLSMDVAYKVEPDGRLTASN
                     KLVLNQLEFGEPVPGAPASLPVKLATALLADSQGVIDLDLPISGSLNDPQFSVGPIIV
                     KAIVNLIGRAITAPFTLLARALGGGAGGDDMANVAFAPGSAQLQPKAREQLDKVAKAL
                     IDRPQLKLTVVGTSSLAAEREGYKRERLKAMVAAEQRAAAPAAAVASAPLPAASQDAA
                     QTIAAGAGQTSAGGRNDSASVADADYPRLLRQLYRRADLRGKPRNAIGMTRDVPVPEM
                     EALLLAQIDVGDDAMRQLALQRGVAVKDYLAGKKLQPERLFLGAPRASATSPASAPAS
                     AASAAPAATGPWSPRAELALTAR"
     gene            1692..>2223
                     /locus_tag="PKV30_13075"
     CDS             1692..>2223
                     /locus_tag="PKV30_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRL31183.1"
                     /translation="MFPFSRDKQDPQPEQAAAAPKAPEAHPLDALTGGVFSAATSGER
                     AQRVRDWLTTDPSPEQMQEVFKELSSKDKGAAKALREKLDELRRAKGQEAVAAEWESK
                     ALALLQSARLNIADALAWQRDAAKAGAPLSREPLSSLRTQVVERIKKVEDLQQRVMVQ
                     REAAALLAQRIELLSTK"
BASE COUNT          378 a          825 c          729 g          291 t
ORIGIN      
        1 ggtcgaggtc aaggacacct tgctgagtga cttctacgcg cgcctgatca ttcaccccga
       61 tggccgcatc aacctgcagg acgtgatcaa gtcgaaggat ggcgcgcccg ccgtgtcccc
      121 ggccgccgca cccgccgccg cccgtaccgc gcccggcatg ccggcagccg gcgagaacaa
      181 cggcgaggct agcaattcaa tagctgccag cgcaagccag accagcgcca gcgccccaaa
      241 accccaacaa ctcgatccgc tggcgccggt catccgcttc gggccgatcc gcatggccag
      301 cgggcacgtg ctgttctccg accgcttcat tcgccccaac tactcggccg atttgactga
      361 gttgaacggc tctttgagcg cctccagctc ggtcgccccg gccggcgcgc cgcagatggc
      421 cgatctgaac ctcaccggcc gcgccgaagg caccgccgcg ctgcaggtgg gcggcaagct
      481 caacccactg gccaagccgc tggcgctgga cattcaggcc aaggtcaccg atctggaact
      541 gccgccgctg tcgccttatt ccgtcaagta cgccggccac ggcatcgagc gcggcaagct
      601 cagcatggac gtggcctaca aggtcgagcc cgacgggcgc ctgacggcca gcaacaagct
      661 ggtgctgaac cagctggagt ttggcgagcc ggtgccgggc gcgccggcca gcctgccggt
      721 taagctggcc accgcgctgc tggccgacag ccagggcgtg atcgatctcg atttgcccat
      781 cagcggctcg ctgaacgacc cgcagttttc cgtcgggccg atcatcgtca aggccatcgt
      841 caatctgatt ggccgcgcca tcaccgcgcc cttcacgctg ctggcgcgag cgctgggcgg
      901 cggcgcgggc ggtgatgaca tggccaacgt ggcctttgcg cccggcagcg cgcaattgca
      961 gccgaaggcg cgcgagcagc tcgacaaggt ggccaaggca ctcatcgatc ggccgcagct
     1021 caagctgacg gtggtgggca cgtccagcct ggccgccgag cgcgagggct acaagcgcga
     1081 gcgcctgaaa gccatggtgg cggccgagca gcgcgccgcc gcgccggccg ctgccgtggc
     1141 atcggcgccg ttgcccgccg catcgcagga tgctgcacaa accatagctg ctggcgcagg
     1201 tcagacaagc gctggcggcc gaaatgattc agcatctgtc gccgacgccg actacccgcg
     1261 cctgctgcgc cagctgtacc gccgcgccga cctgcgcggc aagccgcgca acgccatcgg
     1321 catgacgcgc gacgtgccgg tgcccgagat ggaggcgctg ctgctggcac agatcgacgt
     1381 cggcgacgac gccatgcgcc agttggcgct gcagcgcggc gtggccgtca aggactacct
     1441 ggccggcaag aagctgcagc ccgagcggct gtttctgggc gcgccgcgcg ccagcgccac
     1501 gtcgcccgcc agcgcaccgg ccagcgccgc cagcgctgcg ccagcggcca ccggcccgtg
     1561 gtcgccgcgc gccgaactgg cgctgaccgc ccgctaaagc gctgcccgcc ggcgcccacc
     1621 caaatcccgg cgaaaataca cgccttcagc acgcccggcg gcgtgcgccg ttgacttccc
     1681 accttcacag catgttcccc ttttcccgcg acaaacaaga cccgcaaccc gagcaagcgg
     1741 ccgccgcgcc caaggcgccc gaagcccatc cgctcgacgc gctgacgggc ggcgtgttct
     1801 cggccgccac gtcgggcgag cgcgcccagc gcgtgcgcga ctggctgacc accgacccga
     1861 gccctgagca aatgcaggag gtgttcaagg agctgtcgtc caaggacaag ggcgccgcca
     1921 aggcgctgcg cgaaaagctc gacgaactgc gccgcgccaa gggccaagag gcggtggccg
     1981 ccgaatggga atcgaaagcg ctggcgctgt tgcaatcggc gcgcctgaac atcgccgacg
     2041 ccctggcctg gcagcgcgac gccgccaagg ccggcgcgcc gctctcgcgc gagccgctgt
     2101 ccagcctgcg cacgcaggtc gtcgagcgca tcaagaaagt cgaggatttg cagcagcgcg
     2161 tgatggtcca gcgcgaagcc gccgccctgc tggcgcagcg catcgagctg ctgtccacca
     2221 agc
//
LOCUS       DAOMVO010000381         2221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14414_length_2221_cov_13.819021, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000381 DAOMVO010000000
VERSION     DAOMVO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14414_length_2221_cov_13.819021"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..530)
                     /locus_tag="PKV30_13080"
     CDS             complement(210..530)
                     /locus_tag="PKV30_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31184.1"
                     /translation="MSKRILRTVPAAVLVTTALAWNLPAAAQDTPAMPPGTAASGAMQ
                     KSPAPRANSETGRASPATSGAAFGGGAPATPALSPAMPADAASAPGSRRPAQPGMGTG
                     TSKP"
     gene            946..1212
                     /gene="infA"
                     /locus_tag="PKV30_13085"
     CDS             946..1212
                     /gene="infA"
                     /locus_tag="PKV30_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRL31185.1"
                     /translation="MAKEELIEMQGIVNEVLPDTRFRVTLDNGHELVAYSAGKMKKNH
                     IRILAGDRVTLELSPYDLTKGRINFRHLERRGPAPTGGGGQRRR"
     gene            complement(1410..>2221)
                     /locus_tag="PKV30_13090"
     CDS             complement(1410..>2221)
                     /locus_tag="PKV30_13090"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRL31186.1"
                     /translation="AELLVVMRVYFEKPRTTVGWKGYINDPHRDGSYAINEGLQMARR
                     LLLDVLAIGLPVGCEFLDLLSPQFISDLVSWGAIGARTTESQSHRQLASGLSCPVGFK
                     NGTDGGVKVAADAIVAAQAPHAFLGLTKMGQAAVFETRGNQDCHVILRGGKVPNYSKA
                     DVDAACALLKSNGLREQVMVDVSHANSSKQHAKQIEVAADVAAQVAAGDGRIMGVMIE
                     SHLEEGRQDIVAGQQLKPGVSVTDACISFAQTVPVLDGLAEAVRARRSKRG"
BASE COUNT          374 a          765 c          747 g          335 t
ORIGIN      
        1 cggcgcccgc cgtgcgcaaa cgcgcgccgc cgaaggtggt ggaagaaaag cgcgaagatt
       61 gagcgcttgc tcttgatttg atagcttcct gcgcttgcca gacaagcact ggcgtccaaa
      121 ataaccatga aaaaagcccc ggccgcacgg gccggggctt gggggcggat ggcgtcagcg
      181 gcgctgtcgc atcacgcgcg cggcggcgat cacggctttg atgtgccggt gcccatgccg
      241 ggctgggcgg gcctgcgcga acccggcgcc gatgcggcat cggccggcat ggcggggctc
      301 agggcgggcg tggccggtgc gccgccgcca aaggccgcgc cgctggtggc cgggctggcg
      361 cgccccgttt cgctgttggc gcgcggcgcg ggcgacttct gcatcgcgcc agacgccgcg
      421 gtgcccggcg gcatggccgg cgtgtcctgg gccgcggcgg gcagattcca ggccagcgcg
      481 gtggtcacca gcacggcggc gggaacggtg cgcaaaatgc gtttggacat ggggtgaatg
      541 acctttcggt ggttgaacac aggcggcagt ctgcccgctg gcgccgttac atcccgggcg
      601 caaatgttgg cgttgtaacg atgtgaatgc gcactgacat tgaaaattga cagcgaaaca
      661 aagccaccgc cacggcccgg cgccccgcca gcgcacggtc ccgcgctggc gcacgcgggg
      721 cggcgccgct aaaataccgg gttccccttg aagacccgat gggcgccggc gcgcggcttt
      781 tggtcgccac ccgattcgcg cccctgccct gtggcgcgcc ctggccggcc cgccccgcgg
      841 ccgccgccac ggcgcgcggg cacccgagaa ccgatgcgcg cgcggcaacc cgccggccgc
      901 gcccgacaac gcaacgcaag aaatcgaccg cattaggaga aatggatggc gaaggaagaa
      961 ctgatcgaga tgcaaggcat cgtcaacgaa gtgctgccgg acacgcgctt tcgcgtcacg
     1021 ctggacaacg ggcacgagct ggtggcgtat tccgccggca agatgaagaa gaaccacatc
     1081 cgcatcctgg ccggtgaccg ggtgacgctg gagctgtcgc cctacgacct gaccaagggc
     1141 cgcatcaact tccgccacct ggagcgccgc ggccccgcgc ccaccggcgg cggtggccag
     1201 cgccgccgct gaacgtttca aacaaaaaac ggcgccagcg cgcttgggac aagcgcgggc
     1261 agctccttga aatagagcta aaccgaaaag caatccaacg ccgttcgtcc tgagcctgtc
     1321 gaaggacttg gcgccagagg aacaggcttc gacaagctca gccccaacgg gcgcggaatg
     1381 ggggcttgtt ggtgggatca gccacagcgt cagccgcgtt ttgaccgccg cgcccgcacc
     1441 gcctcggcca agccatcgag caccggcacc gtctgcgcaa agctgatgca ggcgtcggtc
     1501 accgacacgc cgggcttcag ttgctggccg gccacgatgt cctgccggcc ttcctccaga
     1561 tggctttcga tcatcacgcc catgatgcgg ccgtcgcccg ccgccacctg agccgccacg
     1621 tcggccgcta cctcgatctg tttggcgtgc tgcttgctcg aattggcgtg cgacacatcg
     1681 accatcacct gctcgcgcag cccgttcgat ttgagcagcg cgcacgccgc atccacgtcg
     1741 gcctttgaat aattgggcac cttgccgccg cgcaggatga cgtggcaatc ctggttgccg
     1801 cgcgtctcga acacggccgc ctgccccatc ttggtcagcc ccagaaaggc gtgcggcgcc
     1861 tgggcggcga cgatcgcatc ggccgccacc ttcacgccgc cgtcggtgcc gttcttgaag
     1921 ccgaccgggc agctcaggcc gctggccagc tggcggtggc tctggctctc ggtggtgcgc
     1981 gcgccaatcg cgccccagct caccaggtcg ctgatgaact gcggactcag caggtcgaga
     2041 aactcgcagc cgaccggcag gccgatggcc agcacgtcca gcagcaggcg gcgcgccatt
     2101 tgcagccctt cgttgatggc atagctgccg tcgcggtgcg ggtcgttgat gtagcccttc
     2161 cagcccacgg tggtgcgcgg tttttcgaag tacacgcgca tcaccaccag cagctcggca
     2221 g
//
LOCUS       DAOMVO010000382         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14727_length_2185_cov_15.678873, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000382 DAOMVO010000000
VERSION     DAOMVO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14727_length_2185_cov_15.678873"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKV30_13095"
     CDS             <1..357
                     /locus_tag="PKV30_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HRL31187.1"
                     /translation="SLFTLAAFILLAAAYVPRNQIKARLHHPMVLAVKLWAFGHLLAT
                     AKLADVVLFGAFLLWAILDFRAARQRDRALGTVYAPGAVGGTLIAVVLGVVAWAAFAF
                     WLHAAWIGVAPLGRTL"
     gene            complement(424..711)
                     /locus_tag="PKV30_13100"
     CDS             complement(424..711)
                     /locus_tag="PKV30_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRL31188.1"
                     /translation="MSDETVTRQLRIHGRVQGVYYRASMVAEATRLGLHGWVRNRADG
                     SVEALAQGGAPAVQALVDWARRGPPQALVERVDVADAEPQTLPPFEQRATA"
     gene            754..2004
                     /locus_tag="PKV30_13105"
     CDS             754..2004
                     /locus_tag="PKV30_13105"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRL31189.1"
                     /translation="MLRWLTRAVLALIALAVLATGVALWRYASYAPADPPIAPDAKAQ
                     AYFIDDYAPARAAFLDQARALAGRFERVEQFAIPVASAQTKDLFVDGLYVPAQRAPKR
                     LLILSSAVHGVEGPAGSAVQRLFMGEFMTPEALQDTGVLLLHAVNPYGFAVRRRVTEQ
                     NVDLNRNASTTNALYLTDNAGYPLVDALINPPDAVDTGAWAHRLFLLRAVAMIAQHGM
                     PPLRQAVLQGQYAYPKGIYYGGGALAPQLQALAPRLEKIINDYPLSMSIDLHTGYGAR
                     GQLHVFLNPPADARTRQGLEQVFAGHPIDWGSGKDFYTVTGDVTGWIGTLRRSGPHLP
                     ALFEYGTLDSQTTLGAIKSLQISVLENQGVQHGWASPADQARVAQDYREMFNPSAPAW
                     RTKVITDSRALLEQVMVRLPTLQP"
     gene            2001..>2185
                     /locus_tag="PKV30_13110"
     CDS             2001..>2185
                     /locus_tag="PKV30_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRL31190.1"
                     /translation="MKSPARALPRIDPARCTGCGWCIGVCAPHVLSLQAGGGRKTSTL
                     HDAAGCTGCALCAVRCP"
BASE COUNT          304 a          773 c          757 g          351 t
ORIGIN      
        1 tcgctgttca cgctggcggc cttcatcctg ctggcggccg cctacgtgcc gcgcaaccag
       61 atcaaggcca ggttgcacca cccgatggtg ctggccgtca agctgtgggc gttcgggcat
      121 ttgctggcca ccgccaagct ggccgatgtg gtgttgtttg gcgccttcct gctgtgggcc
      181 atcctcgact ttcgcgctgc gcgccagcgc gaccgggcac tgggcacggt ctacgcgccg
      241 ggcgccgtgg gcggcacgct gatcgcggtc gtcctcggcg tggtggcgtg ggcggcgttt
      301 gcgttctggc tgcacgcggc gtggatcggc gtcgcgccgc tgggccgcac actctgattt
      361 tgatagctgc tcgcgctggt cagacaagcg ctggcgcctt aaaacgcttg aaacaccgtt
      421 gattcaggcg gtcgcgcgct gctcgaacgg cggcagcgtt tgcggctcgg catcggctac
      481 atcgacccgc tcgaccagcg cctgcggcgg cccgcgccgc gcccagtcga cgagcgcctg
      541 cacggctggc gcaccaccct gcgccagcgc ctccacactg ccgtcggcgc ggttgcgcac
      601 ccagccgtgc aggcccaggc gcgtcgcctc ggccaccatg ctggcgcggt agtaaacgcc
      661 ctgtacgcgg ccgtggatgc gcagctggcg ggtgaccgtc tcgtcgctca tgtggggcat
      721 catacgaagc gcatcgcatg gaagggattg cacatgcttc gttggctgac acgcgccgtt
      781 ctggcattga tcgcgctggc cgtgctggcc acgggcgtgg cgctgtggcg ctacgccagc
      841 tacgcgccgg ccgacccgcc catcgcgccc gacgccaagg cgcaggccta tttcatcgac
      901 gactacgcgc cggcgcgggc ggcctttctg gatcaggcgc gcgcgctggc ggggcgcttc
      961 gagcgcgtcg agcaattcgc cattccggtc gccagcgcgc agaccaagga cttgttcgtc
     1021 gacggcctgt acgtgccggc gcagcgcgcg ccgaaacgcc tgctcatcct ctcatccgcc
     1081 gtgcacggcg tcgaagggcc ggccggcagc gcggtgcagc ggctgttcat gggcgagttc
     1141 atgacgcccg aagcgctgca agacaccggg gtgctgctgc tgcacgccgt caacccctac
     1201 ggctttgcgg tgcgccgccg cgtgaccgag cagaacgtag atctcaaccg caatgcctcg
     1261 acgacgaatg cgctatacct gaccgacaac gccggctacc cgctggtcga cgcgctcatc
     1321 aacccgcccg atgcggtgga cacgggcgcc tgggcccacc ggctgttttt gctgcgcgcg
     1381 gtggccatga tcgcgcagca cggcatgccg ccgctgcggc aggcggtgtt gcaggggcaa
     1441 tacgcgtacc cgaagggcat ctactacggc ggcggcgcgc tggcgccgca gttgcaggcg
     1501 ctggccccgc ggctggaaaa aatcatcaac gactacccgc tgtcgatgag catcgacctg
     1561 cacaccggct acggcgcgcg cgggcagttg cacgtgtttt tgaacccgcc cgccgacgcg
     1621 cgcacgcgcc aggggctgga gcaggtgttt gccggccacc cgatcgactg gggttcgggc
     1681 aaggacttct acaccgtcac cggcgacgtc acgggctgga tcggcacgct gcggcgcagc
     1741 ggcccgcacc tgccggcgct gttcgagtac ggcacgctcg acagccagac cacgctgggc
     1801 gccatcaagt cgctgcagat cagcgtgttg gaaaaccagg gcgtgcagca cggctgggcc
     1861 agccccgccg accaggcccg cgtggcgcag gactaccgcg agatgttcaa ccccagcgcg
     1921 cccgcctggc gcaccaaggt catcaccgac agccgtgcgc tgctggagca ggtgatggtg
     1981 cggttgccga cgctgcagcc atgaagtcgc ccgcccgcgc cctgccgcgc atcgaccccg
     2041 cgcgctgcac cggctgcggc tggtgcattg gcgtgtgcgc gccgcacgtg ctgtcgctgc
     2101 aggcgggcgg cgggcgcaag acttcgacgc tgcacgacgc cgccggctgc accggttgcg
     2161 cgctgtgtgc ggtgcgctgc ccgtt
//
LOCUS       DAOMVO010000383         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_14780_length_2181_cov_10.277516, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000383 DAOMVO010000000
VERSION     DAOMVO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14780_length_2181_cov_10.277516"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="surE"
                     /locus_tag="PKV30_13115"
     CDS             complement(<1..625)
                     /gene="surE"
                     /locus_tag="PKV30_13115"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRL31191.1"
                     /translation="MKILLCNDDGYQATGIVAFYEALKALPDVQVEVVAPEHNNSAKS
                     NALTLRAPLYVYQAANGFRYVNGTPADCVHIALTGLLDYRPDLVVSGINNGANMGDDT
                     IYSGTVGAAMEGYLFGIPAIAFSQIEKGWGHIDAAATKAAELVQRIGKHQLEGGKPWL
                     LNVNIPNLPLGEIKAPRVCRLGRRHAAERVITMDSPHGDTMYWIGGAG"
     gene            1012..1992
                     /locus_tag="PKV30_13120"
     CDS             1012..1992
                     /locus_tag="PKV30_13120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRL31192.1"
                     /translation="MHAWLCENPVGVDALTWKELPTPQPGKGQVLIRIQAASLNFPDL
                     LIVQNKYQMKPELPFVPGSEYAGVIEAVGEGVTDLKVGQAVACLSGTGGFGTHTLAPA
                     KLCMPLPPGFPPVDAAAFIMIYATSHHALMDRGELKAGETVLVLGAAGGVGMSAIQIA
                     KQAGAKVIAAASTDEKCAFCKTLGADDTINYSTEDLRERLKALTGGKGPDVIYDPVGG
                     EFTEAAFRSIAWRGRHLVVGFAAGEIPAIKINLPLIKGASLVGVFWGDFARREPAANA
                     AMMQTLASWYAQGKIKPVIDRQMPMSELKAAYARMGSREVMGKLVLVNEA"
BASE COUNT          378 a          704 c          721 g          378 t
ORIGIN      
        1 ggccggcgcc gccgatccag tacatggtgt cgccgtgcgg gctgtccatg gtgatgacgc
       61 gctcggccgc atggcgccgg cccaggcggc agacccgcgg cgccttgatc tcgcccagcg
      121 gcaggttggg gatgttcacg ttcagcagcc agggtttgcc gccttccaac tggtgtttgc
      181 caatgcgctg caccagctcg gccgccttgg tggctgcggc gtcgatgtgg ccccagcctt
      241 tttcgatctg cgagaaggcg atggccggga tgccgaacag ataaccctcc atcgccgcgc
      301 ccacggtgcc agagtaaatg gtgtcgtcgc ccatgttggc gccgttgttg atgcccgaga
      361 cgaccaggtc gggccgatag tcgagcaagc ccgtcagcgc gatgtgcacg cagtcggccg
      421 gcgtgccgtt gacgtagcga aagccgttgg ccgcctggta cacgtacagc ggcgcgcgca
      481 gcgtcagcgc gttggacttg gcgctgttgt tgtgctcggg cgcgacgact tcgacctgca
      541 cgtcgggcag cgccttcagg gcttcgtaaa aggccacgat gccggtggcc tggtagccgt
      601 cgtcgttgca gagcaggatc ttcatgaagg gtgtcgcgcg cagatgcaac cgattgtaga
      661 ggccacctcc gcctcgcccg gcgcaacgtc gccgctggga cacagggaca ttcccgcgcg
      721 cttctatgat cctggaaacg gcagatgaac gtgcccgagc gtgccgtgat gggcgtgtaa
      781 ggcaaggcgc gaggacgccg caggctcctg cctgcaagga cgagcaacgc cgcatggcgc
      841 ggccagcgcg gtgtgatcgg gcacgtagcg cgctcaccga aaaggtgaat gccatcgaag
      901 tcaaaaacgg tcacgcaaca caagcgttta tcgagatatg aaagcggtca tctgcctttt
      961 ctaggatgat cgatcacgtc atttttcagc ccccacacga ggaaaccatt catgcacgcc
     1021 tggctttgcg aaaaccccgt cggtgtcgac gccctgacct ggaaggaact gcccacgccg
     1081 cagccgggca agggtcaggt cctgatccgc atccaggccg ccagcctgaa ctttcccgac
     1141 ctgctgatcg tgcagaacaa gtaccagatg aagcccgagc tgcccttcgt gcccggctcc
     1201 gagtacgccg gcgtgatcga ggcggtgggc gaaggcgtga ccgacctgaa agtcggccag
     1261 gccgtggcgt gcctgagcgg caccggcggc tttggcaccc acaccctggc gccggccaag
     1321 ctgtgcatgc cgttgccgcc cggttttccg ccggtggacg cggccgcttt catcatgatc
     1381 tatgccacca gccaccacgc gctgatggac cgcggcgagc tgaaggctgg cgaaaccgtg
     1441 ttggtgctgg gcgccgccgg cggcgtgggc atgtcggcca tccagatcgc caagcaggcc
     1501 ggcgccaagg tgattgccgc cgcctcgacc gacgagaaat gcgccttctg caagaccttg
     1561 ggcgccgacg acaccatcaa ctacagcacc gaggatttgc gcgagcgcct gaaagcgctg
     1621 accggcggca agggcccgga cgtcatctac gacccggtgg gcggcgaatt cactgaagcc
     1681 gccttccgct ccatcgcctg gcgcggccgg cacctggtgg tcggttttgc ggcgggcgag
     1741 attcccgcca tcaagatcaa cctgccgctc atcaagggcg ccagcctggt tggcgtgttc
     1801 tggggcgact ttgcgcgccg cgagccggcc gccaacgccg ccatgatgca gacgctggcc
     1861 agctggtacg cccaaggcaa gatcaagccc gtgatcgacc gccagatgcc catgagcgag
     1921 ctgaaagccg cctacgcgcg catgggctcg cgcgaggtga tgggcaagct ggtgctggtc
     1981 aacgaagcgt agttgcttcc ttttctatag ctgctggcgc acgccctgct tgcgccagtg
     2041 gccgaattgg caagtatttt tctgcggatg cggccttgtc cttcaagcgc gtgcgccgcg
     2101 cgccgacccg gcgtgccgag cggtttggcg agcatggagg ccgagaaatt ttcaagctgc
     2161 cggccatgac cacgctgacc c
//
LOCUS       DAOMVO010000384         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15003_length_2158_cov_12.850689, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000384 DAOMVO010000000
VERSION     DAOMVO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15003_length_2158_cov_12.850689"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..940
                     /locus_tag="PKV30_13125"
     CDS             5..940
                     /locus_tag="PKV30_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL31193.1"
                     /translation="MDDLNDLAYFAHVAEHGGFAAAERATGIPKSKLSRRIAELEARL
                     GVRLIQRSTRRFAVTDIGQRTLQHARAMLAEADAAQALARQETAAPRGTVRLSCPPAL
                     LQHAVSDMLARFLNAWPQVTLQVQSTNRNVDVWQDGVDLALRVRPTGAALPQDEIVKP
                     LALSPHQLVAAPALLTNAAPPATPAELARLPTLALGNSPDEALWRLSGPDGATADVPL
                     APRLVVDDMGALLCGALAGVGCAALPRMMTHEALARGDLQTVLPGWAPPAGVVQAAFA
                     SRQGVRPAVRQLLDALAAGFDQMVREGRCLRAPGA"
     gene            complement(1040..1963)
                     /locus_tag="PKV30_13130"
     CDS             complement(1040..1963)
                     /locus_tag="PKV30_13130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL31194.1"
                     /translation="MTGSALLNEPARSARRRALSSLAAAAMLLSLPALAQKGAAPAAS
                     YEPQSGQAGKDVIWLPTPDELVTRMLQLGGVGKNDYVIDLGAGDGKIVIAAAKQFGAR
                     GHGIEYNPDMVAHATRRAKEAGVSDRARFEKADIFESDFSNADVITMYLLPTLNLKLR
                     PTLMKLKPGTRLVTHAFNMGGWPPDETSRAGGANAYLWLVPANVSGQWQLSYPQGGKQ
                     LSQAMTVERQRYQFPEGTVVLGSADTSLRDARVIGDRVRFAFTGTDGVLRTFDGKVNG
                     GRIDGQVSDGHKSEKFSATRKGEGTAIETVD"
BASE COUNT          301 a          757 c          763 g          337 t
ORIGIN      
        1 acccatggac gacctcaacg acctcgccta cttcgcccac gtggccgagc atggcggctt
       61 tgccgctgcc gagcgcgcca ccggcatccc caagagcaag ctgagccgcc gcatcgccga
      121 gctggaagcg cggctgggcg tgcggctgat ccagcgcagc acgcggcgct ttgccgtcac
      181 cgacatcggc cagcgcacgc tgcagcacgc gcgcgccatg ctggccgagg ccgacgccgc
      241 gcaggcgctt gcgcggcagg agacagcggc gccgcgcggc acggtgcgcc tgtcgtgccc
      301 gccggcgctg ctgcagcatg cggtgagcga catgctggcg cgctttctca acgcctggcc
      361 gcaggtcacg ctgcaggtgc agtcaaccaa ccgcaacgtc gatgtgtggc aggacggcgt
      421 cgacctggcg ctgcgcgtgc gccccaccgg cgccgcgctg ccgcaggacg agatcgtcaa
      481 gcccttggcg ctgagcccgc accagctggt ggccgcgccg gcgctgttga ccaacgccgc
      541 gccgccggcc acgccggccg agctggcgcg cctgcccacg ctggcgcttg gcaattcgcc
      601 cgacgaggcg ctttggcggc tgagcggccc ggatggcgcc acggccgacg tgccgctcgc
      661 gccgcgcctg gtggtggacg acatgggggc gctgctgtgc ggcgcgctgg ccggtgtcgg
      721 ctgcgccgcg ctgccgcgca tgatgacgca cgaggcgctg gcccgcggcg atttgcaaac
      781 cgtgctgccc ggctgggcgc cgccggccgg cgtggtgcag gccgcctttg ccagccggca
      841 gggcgtgcgg ccggcggtgc ggcaattgct ggatgcgctg gcggccggtt tcgatcagat
      901 ggtgcgcgaa gggcgctgcc tgcgggcgcc cggggcgtga ggccgtttgc tcctgtaaac
      961 atagctgcca gcgctttctc agcaagcgcc agggccgatt tcaagcccga agatcacgcg
     1021 caggccgcgc gcccgcagct caatccaccg tctcgatcgc cgtcccttcg cccttgcgcg
     1081 tggcgctgaa tttctccgac ttgtggccat cgctcacctg gccgtcgatg cgcccgccgt
     1141 tgaccttgcc gtcgaaggtg cgcagcacgc cgtcggtgcc ggtgaaggca aagcgcacgc
     1201 ggtcgccgat cacgcgcgcg tcgcgcagcg aggtgtcggc gctgccgagc accaccgtgc
     1261 cttcggggaa ctggtaacgc tggcgctcca ccgtcatggc ctggctcagc tgcttgccgc
     1321 cctgcgggta gctgagctgc cactggccgc tgacgttggc cggcaccagc cacaagtaag
     1381 cgttggcacc gcccgcgcgc gaggtctcgt cgggcggcca gccgcccatg ttgaaggcat
     1441 gcgtgaccag ccgcgtgccg ggcttgagct tcatcagcgt cggccgcagc ttcaggttga
     1501 gcgtgggcag caggtacatg gtgatgacgt cggcgttgct gaagtcggac tcgaagatgt
     1561 cggccttctc gaagcgcgcg cggtccgaca cgccggcttc cttggcgcgg cgcgtggcat
     1621 gcgcaaccat gtcggggttg tattcaatgc catggccgcg cgcgccgaac tgcttggcgg
     1681 cggcgatgac gatcttgcca tcgccggcgc ccaagtcgat cacgtagtcg ttcttgccca
     1741 cgccgcccag ctgcagcatg cgcgtgacca actcgtcggg cgtcggcaac cagatcacgt
     1801 ccttgccggc ctggccgctt tgcggctcat agctggcggc aggcgcagcg cccttttgcg
     1861 ccaacgcggg cagcgacagc aacatggctg cggcggcaag tgacgacagc gcgcggcggc
     1921 gggcgctgcg agcgggttca ttcaagagcg cggatccggt catgcgatga gactccttga
     1981 aagtgatgga tgagacgacg ccgccacgcc accaacagcc agcgccacgg tgcgacgcca
     2041 tgttaattgc gcacgcaacc cagggcgccc cgcagacccc gagtgcgctg cgggggtatt
     2101 tcgtttgctc tgttgttgat agctgcccgc gctggatatg cgggcaccgg cggcctaa
//
LOCUS       DAOMVO010000385         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15155_length_2144_cov_14.835328, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000385 DAOMVO010000000
VERSION     DAOMVO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15155_length_2144_cov_14.835328"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..2126)
                     /locus_tag="PKV30_13135"
     CDS             complement(192..2126)
                     /locus_tag="PKV30_13135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRL31195.1"
                     /translation="MREVFGYDAFRGQQQGVIDHAIAGGDALVLMPTGGGKSLCYQVP
                     AIARQRAGLGVTLVISPLIALMHDQVGALHEAGVAAAFLNSTLDWEQTQDIERRLLAG
                     QLTLLYAAPERVGTPRFLGLLDTLHAHGALSLFAIDEAHCVSQWGHDFRPEYRQLTVL
                     HERWPSVPRIALTATADAVTRADIVERLQLQNAEQFVSSFDRPNIRYTIVEKKDATRQ
                     LLQFIQHEHEGEAGIVYCQSRRRVEELAQTLCEAGIDAMPYHAGLPAPERQQNQDRFL
                     RDEGVVMCATIAFGMGIDKPDVRFVAHVDMPKNIEGYYQETGRAGRDGQPADAWMTYG
                     LQDVVNQRRMIDESPAGEDFKQVMVAKLDALLALAEATDCRRARLLGYFGEAMAAPDN
                     AIDSGAAGASPSSAGGPFGTYSRWHCGNCDNCLQPPEVWDATDCARKLLSTIYRVQQM
                     SGLSFGAGHIMDIVRGKSTDKVAQFGHEQLSTFGIGAEFSEPQLRGVLRQLIAVGALQ
                     VDAQAFNTLKLLPEARPILKGEQPVRLRAATAPAPRERVRKPRGERAARSGATPPAAA
                     ELDAAAQTRFAALKAWRAEVAREHNLPAYVIFHDATLAAIAARAPQSLSDLQGIGGIG
                     ATKLERYGDEVLHVSTSSTG"
BASE COUNT          322 a          706 c          744 g          372 t
ORIGIN      
        1 gaccagcacc ttgccctcgc gccccacggt gcgcatcacg ccgcgccagc cgagcttttt
       61 gacgtcggac gcgggcgtgc ggggcaactg atcaaactcg gccaggtggt cgaaactcag
      121 ggcgtaggcc atggttcaat ccttcacgaa aaggacaatg tggccattat gccctttttt
      181 ggaaatttgg gtcatccggt ggacgaggtg cttacgtgca gcacctcgtc gccatagcgc
      241 tccagcttgg tcgcgccgat gccgccgatg ccttgcaggt cgctcagcga ctgcggcgcg
      301 cgcgcggcga tggcggccag ggtggcgtcg tgaaagatca cgtaggcagg caggttgtgc
      361 tcgcgcgcca cttcggcgcg ccaggctttc agtgcggcaa agcgggtttg tgcggcggcg
      421 tccagttcgg cggcggcggg gggcgtggcg ccgctgcgcg cggcacgctc gccgcgcggc
      481 ttgcgcacac gctcgcgcgg ggccggcgcg gtggcggcgc gcaggcgcac gggttgttcg
      541 cctttcagaa tcggtcgcgc ttcgggcagc agcttgagcg tgttgaaggc ctgcgcatcg
      601 acctgcagcg cgccgacggc gatcaactgg cgcagcacgc cacgcagttg cggctcgctg
      661 aattcggcgc cgatgccgaa cgtggacagc tgctcgtgcc cgaattgcgc caccttgtcg
      721 gtcgatttgc cgcgcacgat gtccatgatg tggccggcgc caaagctcag gccgctcatt
      781 tgctgcacgc ggtagatggt cgagagcagc ttgcgcgcgc aatccgtcgc gtcccacacc
      841 tcgggcggct gcaggcagtt gtcgcagttg ccgcagtgcc agcgtgaata tgtgccaaat
      901 gggcctccag cgcttgacgg acttgcgcca gcagctcctg aatcaatagc attgtcaggc
      961 gctgccatcg cctcgccaaa atagcccagc agccgcgcgc ggcggcaatc ggtggcctcg
     1021 gccagcgcca gcagcgcatc gagcttggcc accatgacct gcttgaagtc ttcgccggcc
     1081 gggctctcgt cgatcatgcg gcgctggttc accacgtctt gcaggccgta ggtcatccag
     1141 gcgtcggccg gctgaccgtc gcggccggcg cggccggtct cctggtaata gccttcgatg
     1201 ttcttgggca tgtccacatg cgcgacaaag cgcacgtcgg gcttgtcgat gcccatgccg
     1261 aaggcaatcg tcgcgcacat caccacgccc tcgtcgcgca aaaagcggtc ctggttctgc
     1321 tggcgctcgg gcgcgggcag gccggcgtgg tagggcatgg cgtcgatgcc ggcttcgcac
     1381 agcgtctgcg ccagctcttc cacgcggcgg cgcgactggc agtagacgat gccggcctcg
     1441 ccctcgtgct cgtgctggat gaactgcagc agctggcgcg tggcgtcctt cttctcgacg
     1501 atggtgtaac ggatgttggg ccggtcgaag ctgctgacga attgctcggc gttttgcagc
     1561 tgcagccgct cgacgatgtc ggcgcgcgtt accgcgtcgg cggtggccgt gagggcgatg
     1621 cgcggcacgc tcggccagcg ctcgtgcagc accgtcagct gacggtattc gggccggaaa
     1681 tcgtggcccc actggctcac gcaatgtgct tcgtcgatgg cgaacaggct caaggcgccg
     1741 tgcgcgtgca gcgtgtccag caggcccaga aagcgcggcg tgcccacgcg ctcgggcgcg
     1801 gcgtacagca gcgtcagctg gccggcgagc aggcgccgct cgatgtcctg cgtttgctcc
     1861 cagtcgagtg tggaattgag gaaagccgcg gccacgcccg cctcgtgcag cgcgcccacc
     1921 tggtcgtgca tcagcgcgat caggggcgaa atcaccagcg tcacgcccag cccggcgcgc
     1981 tggcgggcta tggccggaac ctggtaacaa agcgacttgc cgccgcccgt gggcatcagc
     2041 accagcgcgt cgccgccggc gatggcgtgg tcaatcacac cctgttgctg gccgcgaaag
     2101 gcgtcgtagc cgaacacctc gcgcaggatg gtcgcgggcg ccga
//
LOCUS       DAOMVO010000386         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15163_length_2143_cov_11.409483, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000386 DAOMVO010000000
VERSION     DAOMVO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15163_length_2143_cov_11.409483"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKV30_13140"
     CDS             <1..775
                     /locus_tag="PKV30_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HRL31196.1"
                     /translation="AAAARKPWGAMLGGLAAGLGLAWLANSLGLGPAFANLLLFLLLG
                     VVVMAVIGMIRRSRAGASQAGGLAYQGAAGNPVTPRQYNPTKVGNDASARPWEGQSAA
                     FDGSATTGAAAGGSIIGSGLSGPQNWGVPADFDVSGFTEAAKRNFITLQDAWDRSDIP
                     ALRAMMTDGMLAEIQSHLAEREREAPGQVNKTEVQSLNAQLLGIEELDDTYMASVEFS
                     GVMVEDPSAGPTPFHEVWNMTKPRHGRGGWLVAGVQALQ"
     gene            949..1488
                     /locus_tag="PKV30_13145"
     CDS             949..1488
                     /locus_tag="PKV30_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31197.1"
                     /translation="MVTQSPFSLLSDALGKLGDQLKAPAWLVDEVQHRTVLFINHVLM
                     QEPEAQARLKRQSGRVVRLQWRDFSMQLAATPAGLWELAPAATPDLLLTVTDTSPFDL
                     AKNALRGDKPGVRIEGDVEFAGEVNWLIENVRWDAEEDLSRFIGDAPAHTLGKIGRSV
                     AEALRSFVSKLPGQDKGGA"
     gene            1485..>2143
                     /locus_tag="PKV30_13150"
     CDS             1485..>2143
                     /locus_tag="PKV30_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRL31198.1"
                     /translation="MTRFARAVTIVWIAMRYGLDELVLTSFQHPWLRLTARVASMGRD
                     LSAPRGQRLREALQKLGPIFVKFGQVLSTRRDLLPLDVADELALLQDQVPPFPGDVAV
                     ATIERAFRKPLDEIFTSFDRTPVASASIAQVHFATVRDKNGREREVAVKVLRPGMLKV
                     IDQDLALIRMMAAWVERLVPDGKRLKPREVAAEFDKYLHDELDLVREAASAAQLRRNM
                     AG"
BASE COUNT          322 a          728 c          764 g          329 t
ORIGIN      
        1 cgctgccgcc gcgcgcaagc cctggggcgc catgctgggc ggcttggccg ccggcctggg
       61 cctggcgtgg ctggccaact cgctgggcct ggggccggcg tttgccaatc tgctgctgtt
      121 tctgctgctc ggcgtggtgg tgatggcggt gatcggcatg atccgccgct cgcgcgccgg
      181 cgcgtcgcag gccggtgggc tggcctacca gggtgccgct ggcaacccgg tgacgccgcg
      241 ccagtacaac ccgaccaagg tcggcaacga cgcctcggcc cgcccgtggg agggccagag
      301 cgccgctttc gacggcagcg cgacgactgg cgccgccgcc ggcggctcga tcatcggatc
      361 gggcctgagc ggcccgcaga actggggcgt gccggccgac ttcgacgtca gcggcttcac
      421 cgaggccgcc aagcgcaact tcatcaccct gcaggatgca tgggaccgct ccgacatccc
      481 ggccctgcgc gccatgatga ccgacggcat gttggccgag atccagtcgc acctggccga
      541 gcgcgagcgc gaggccccgg gccaggtcaa caagaccgag gtgcagtcgc tcaacgcgca
      601 gctgctgggc atcgaggaac tggacgacac ctacatggcc agcgtcgaat tcagcggcgt
      661 catggtcgaa gacccgtccg ccggcccgac gcccttccac gaggtctgga acatgaccaa
      721 gccgcgccac ggccgtggcg gctggctggt ggcgggcgtg caagcgctgc agtaatgctc
      781 ccctgagccg ctgacgcggc ttcccccaag ggggacggcg ccagtggctg ggccaagccc
      841 gttccacggc gcccccgcat ggcctgctcc gcggccgcct caatgcgtgg agcgtcgtct
      901 tctcacgatg ggccggggcc atcggtttgt catgaataat tgggggctat ggtcacacag
      961 tccccttttt ctttgctctc tgacgcgctc ggcaagctgg gcgaccagct gaaggcaccc
     1021 gcctggctgg tcgacgaggt gcagcaccgc acggtgttgt tcatcaacca cgtgctgatg
     1081 caggagcccg aggcgcaggc gcgcctgaag cgccagagcg ggcgcgtggt gcgcctgcaa
     1141 tggcgcgact tctcgatgca gctggccgcc acgccagccg ggctgtggga attggcgccg
     1201 gcggccacgc ccgatttgct gctcaccgtc accgacacct cgccgttcga cctggccaag
     1261 aacgcgctgc gcggtgacaa gcccggcgtg cgcatcgagg gcgatgtgga attcgccggc
     1321 gaggtcaact ggctgatcga gaacgtgcgc tgggacgccg aggaagacct gtcgcgcttc
     1381 atcggcgatg cgccggcgca cacgctgggc aagatcggcc gcagcgtggc cgaagcactg
     1441 agaagcttcg tcagcaagct gccgggccag gacaagggcg gcgcatgacg cgctttgcgc
     1501 gcgccgtcac catcgtctgg atcgccatgc gctacgggct ggatgagctg gtgctcacca
     1561 gttttcagca cccgtggctg cgcctgacgg cccgcgtcgc gtcgatgggg cgcgatttgt
     1621 cggccccgcg cgggcagcgc ctgcgcgagg cgctccaaaa attggggccg atcttcgtca
     1681 agttcggcca ggtgctgtcg acccggcgcg acctgctgcc gctcgacgtg gccgacgaac
     1741 tggcgctgct gcaagaccag gtgccgccgt ttccgggtga cgtggcggtg gcgacgattg
     1801 agcgcgcctt ccgcaagccg ctggacgaga ttttcaccag ctttgaccgc acgccggtcg
     1861 ccagcgcctc catcgcgcag gtgcactttg ccaccgtgcg cgacaagaat ggccgcgagc
     1921 gcgaggtggc cgtgaaggtg ctgcgccccg gcatgctgaa ggtgatcgac caggacctgg
     1981 cgctgatccg catgatggcc gcctgggtcg agcgcctggt gcccgacggc aagcgcctga
     2041 agccgcgcga ggtggcggcc gagttcgaca aatacctgca cgacgagctg gatctggtgc
     2101 gcgaggccgc cagcgcggcg cagctgcggc gcaacatggc ggg
//
LOCUS       DAOMVO010000387         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15244_length_2134_cov_14.393458, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000387 DAOMVO010000000
VERSION     DAOMVO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15244_length_2134_cov_14.393458"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKV30_13155"
     CDS             complement(<1..684)
                     /locus_tag="PKV30_13155"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HRL31199.1"
                     /translation="MEIFDYDHVLLLPRKCRVESRSECDASIEFGGRTFKLPVVPSNM
                     KTVVDEKVCAWLAANGYFYVMHRFDLDNVAFVREMKAKGLFASISVGVKPPDYATIDT
                     LAAESLVPEYITIDIAHGHADTVRDMIRHIKAKLPDAFVIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITKLKTGFGTGGWQLSALKWCARVATKPIVADGGIRSHGDIAKSI
                     RFGATMVMIG"
     gene            848..>2134
                     /locus_tag="PKV30_13160"
     CDS             848..>2134
                     /locus_tag="PKV30_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRL31200.1"
                     /translation="MFSSTDAGAAIELPLLAGLNPEQKAAVTLPPEHALILAGAGSGK
                     TRVLTTRIAWLLQTGQVSPGGVLAVTFTNKAAKEMLTRLTAMLPLNARGMWIGTFHGL
                     CNRFLRAHYKLANLPQAFQILDTQDQLSAIKRLCKQFNVDEERFPPKQLQWFISGCKE
                     DGLRPGDVEVHDAESRKKVEIYQLYEEQCQREGVVDFGELMLRSYELLRDHDAVREHY
                     RRRFRHILIDEFQDTNKLQYAWIKMIAGLPGEGSHSAILAVGDDDQSIYAFRGARVGN
                     MADFVREFGVQHQIKLEQNYRSHSNILDSANELISHNKRRLGKNLRTDAGAGEPVRVL
                     EAPSDFAEAEWVVDELRQLVKSEGYERKEVAVLYRSNAQSRVIETQLFNAGVPYRVYG
                     GLRFFERAEIKHALAYLRQLENPRDDTSFLRVVNFPP"
BASE COUNT          391 a          687 c          676 g          380 t
ORIGIN      
        1 gccgatcatc accatggtgg cgccgaagcg gatgctcttg gcgatgtcgc catggctgcg
       61 gatgccgccg tcggccacga tgggcttggt cgccacgcgg gcgcaccact tgagcgcgct
      121 cagttgccag ccgccggtgc caaagcccgt cttgagcttg gtgatgcaca ccttgcccgg
      181 gcccacgccg accttggtgg cgtcggcgcc ccagttttcc aggtcaatca ccgcctcagg
      241 cgtggccaca ttgccggcga tcacgaacgc atccggcaac ttggccttga tgtggcgaat
      301 catgtcgcgc accgtgtcgg cgtggccgtg cgcgatgtcg atggtgatgt attcgggcac
      361 caggctctcg gccgccagcg tgtcgatcgt cgcgtagtct ggcggcttga cgccgaccga
      421 gattgacgca aacaagcctt tggccttcat ctcgcgcacg aaggccacgt tgtccaggtc
      481 gaaccggtgc atgacgtaaa agtagccgtt ggcggccagc caggcgcaga ctttttcgtc
      541 caccaccgtc ttcatgttcg aaggcaccac cggcagcttg aaggtgcgcc cgccgaattc
      601 gatgctcgcg tcgcattcgc tgcggctttc cacgcggcac ttgcgcggca gcagcagcac
      661 gtggtcgtag tcgaagattt ccatggtttc caagctccat caaggatttc agatcatggg
      721 cgcttggcct ggccacggcg ttttgtgacg ggcacaaaaa accgggccaa gaaatcttgg
      781 gcccggtgat tgattctacg gcttcgccca aacctgtgca ggcaggcgcg ctttctacaa
      841 tcgacccatg ttttcatcca ccgacgcggg cgcggccatc gagttgcccc tgcttgccgg
      901 cctcaacccc gagcagaagg ctgccgtcac cctgccgccc gaacacgcgc tgatcctggc
      961 cggcgccggc tcgggcaaga cgcgcgtgct gaccacgcgc atcgcctggc tgctgcagac
     1021 ggggcaggtg tcgcccggcg gcgtgctggc cgtcaccttc accaacaaag cggccaagga
     1081 gatgctgacg cgcctgacgg ccatgctgcc gctgaatgcg cgcggcatgt ggatcggcac
     1141 ctttcacggg ctgtgcaacc gctttctgcg tgcgcactac aagctggcga atctgccgca
     1201 ggcgtttcag atcctcgata cgcaggatca gctttcggcc atcaagcgcc tgtgcaagca
     1261 gttcaacgtc gacgaagagc gctttccgcc caagcagttg cagtggttca tcagcggctg
     1321 caaggaagac ggcctgcgcc ccggcgacgt ggaggtgcac gatgccgaaa gccgcaagaa
     1381 ggtggagatt tatcagctgt acgaggagca gtgccagcgc gaaggcgtgg tcgacttcgg
     1441 cgaactgatg ctgcgcagct acgagctgct gcgcgaccac gacgccgtgc gcgagcacta
     1501 ccgccggcgc tttcgccaca ttctgatcga cgagtttcag gacaccaaca agctgcaata
     1561 cgcgtggatc aagatgattg ccggcttgcc gggcgagggc agtcacagcg ccattttggc
     1621 cgtgggcgat gacgaccaga gcatctacgc ctttcgcggc gcgcgcgtcg gcaacatggc
     1681 cgacttcgtg cgcgaatttg gcgtgcagca ccagatcaag ctggagcaga actaccgcag
     1741 ccacagcaac atcctcgatt cggccaacga gctgatcagc cacaacaagc ggcgcctggg
     1801 caagaacctg cgcaccgatg ccggcgccgg cgagccggtg cgcgtgctgg aggcgcccag
     1861 cgactttgcc gaggccgaat gggtggtcga cgagctgcgc cagctggtca agagcgaggg
     1921 ctacgagcgc aaggaagtcg ccgtgctgta ccgcagcaat gcgcaaagcc gcgtgatcga
     1981 gacgcagctg ttcaacgctg gcgtgccgta ccgcgtgtac ggcggcctgc gcttcttcga
     2041 gcgggccgaa atcaaacacg cgctggccta tttgcgccag ctggagaacc cgcgcgacga
     2101 caccagcttt ctgcgcgtgg tcaactttcc gccg
//
LOCUS       DAOMVO010000388         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15275_length_2131_cov_16.704721, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000388 DAOMVO010000000
VERSION     DAOMVO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15275_length_2131_cov_16.704721"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PKV30_13165"
     CDS             <1..1019
                     /locus_tag="PKV30_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRL31201.1"
                     /translation="FLGLYRDPTVAEVTSRCDWRIADLIAAEHPVSLYLVVPPSDISR
                     TKPLIRLILNQVGRRLTESLDGSDGIQRRHKLLLMLDEFPALGRLDFFETALAFMAGY
                     GIRSFLIAQSLNQIDKAYGQNHSILDNCHVRVTFATNDERTAKRISETLGTATELRAQ
                     RNYAGHRLAPWLGHLMVSRQETARPLLTPGEVMQLPPDEAVVMVSSVAPIKAKKLRYY
                     ADSNFKRRVLRPPALADGQYADAPPSRPDDWSELAIPAVPAAPATASADGLGSTDDGG
                     PRRQPELTETVTYDPELAAPAADLALLDDDEDLPLPLLRQLDPAMQRTARLASLDPND
                     GIEL"
     gene            1016..1480
                     /locus_tag="PKV30_13170"
     CDS             1016..1480
                     /locus_tag="PKV30_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRL31202.1"
                     /translation="MSQHRLNLFIQPEHAKRLDELAAKKGVSKSSIVAAALASWLSPD
                     AADQREAAIAKRLDRLSRQAERLERDQNIQIETLALFIRYFLTVSTPVPEAHQEAARA
                     HGKARFEQFVEQLGRHLMRGRSLVRDVVEELHPDPMRMDDAAAVAEARERAS"
     gene            1489..>2131
                     /locus_tag="PKV30_13175"
     CDS             1489..>2131
                     /locus_tag="PKV30_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRL31203.1"
                     /translation="MSTEGQSMTATSLDRRIQMLRTAMGPLIAAALEDPDVVEVMLNP
                     DRTLWVDRLSSGRAPMGVELSEADGERIIRLVAAHVGAEVHRGQPLLSAELPETGERF
                     EGILPPAAPGPAFALRKRAIGVIPLERYVVDRMMTSAQAGFLVRAVRERQNVLIAGAT
                     SSGKTTLANALLAEIAATGDRVLVLEDTVELQCAARDHVPLRTRAGVVSMTELV"
BASE COUNT          355 a          744 c          698 g          334 t
ORIGIN      
        1 cgttcctcgg cctgtaccgc gatcccacgg tggccgaagt cacctcgcgc tgcgactggc
       61 gcatcgctga cctgattgca gccgagcatc cggtgtcgct gtacctcgtg gtgccgccat
      121 cggacatcag ccgcacgaag ccgctgatcc ggttgatcct caaccaggtc ggtcggcgat
      181 tgaccgaatc gctcgacggc agcgatggca tccagcgccg ccacaagctg ctgctgatgc
      241 tcgatgagtt cccggcgctg ggccgtctgg acttcttcga gacggcgctg gccttcatgg
      301 cgggctacgg catccgcagc ttcctcatcg cgcagtcgct caaccagatc gacaaagcct
      361 acggccagaa ccattcgatt ctcgacaact gccatgtgcg cgtgacgttc gccacgaacg
      421 acgagcgcac cgccaagcgc atctccgaga cgctaggcac ggccaccgaa ctgcgcgccc
      481 agcgcaacta tgccggccac cggctggccc catggctggg gcacctgatg gtgtcgcgcc
      541 aagaaacggc ccgcccgctg ctgacgccgg gcgaagtcat gcagcttccg cccgacgagg
      601 ccgtggtgat ggtgtccagc gtggcaccga tcaaagccaa gaagctgcgc tactacgcgg
      661 acagcaattt caagcgccgc gtgctgcggc cgcctgcgct tgcggatggg cagtacgccg
      721 acgcgccgcc gtcgcggccc gacgactgga gcgagctggc gattcctgcc gtacccgctg
      781 cacctgccac ggcatccgcc gatggcctgg ggtccaccga tgacggtggc ccgcgccgtc
      841 agcccgaact caccgaaacc gtcacctacg accccgagtt ggccgcgccc gcggccgacc
      901 tggcgctgct cgatgacgac gaggacctgc cgcttcccct tcttcgtcag ctcgatccgg
      961 ccatgcagcg aacggcccgg ctggcatccc tcgaccccaa cgacggaatc gagctatgag
     1021 ccaacaccgc ctgaatctct ttatccagcc ggaacacgca aagcgcttgg acgagctggc
     1081 cgccaagaaa ggcgtgtcca agtcgtccat cgtcgcggcg gcgctcgcat cgtggctgtc
     1141 gcccgatgcc gccgaccagc gcgaggcggc catcgccaag cggttggatc gcctatcgcg
     1201 ccaagccgag cgtctggagc gcgaccagaa catccagatc gaaacgctgg cgctgttcat
     1261 tcgctacttc ctgaccgtca gcacgccggt tcccgaagcg catcaggagg cggcccgcgc
     1321 gcatggcaag gcgcggttcg agcagttcgt cgaacagttg ggccgccacc ttatgcgcgg
     1381 gcgcagtttg gtgcgtgacg tggtggagga actgcacccc gacccgatgc ggatggatga
     1441 cgcggcggca gttgccgaag cgcgggagcg tgcctcatga gcgcccgcat ctccactgaa
     1501 gggcagtcca tgaccgccac gtcgctcgac cggcgcattc agatgctgcg cacggcaatg
     1561 gggccgctga tcgctgccgc gctggaggac ccggacgtgg tggaagtgat gctgaatccc
     1621 gaccggacgc tatgggtaga tcggttgtcc tcgggccgcg cgccgatggg cgtggagctg
     1681 tccgaagcgg acggcgaacg catcatccgg ctggtcgcgg cccacgtcgg agcggaagtc
     1741 catcgcggcc agccgctctt atccgccgag ctgcccgaaa ccggggagcg cttcgagggc
     1801 atcttgccgc cggccgcccc ggggccggcc ttcgccttgc gcaagcgcgc catcggcgtg
     1861 atccctctgg agcggtacgt cgtcgacaga atgatgacca gcgcccaggc gggctttctg
     1921 gttcgggccg tgcgcgagcg gcagaacgtc ctgatcgccg gtgccaccag cagcggcaag
     1981 actacgcttg ccaacgcgct gctggccgag attgccgcca cgggcgatcg cgtgctggtg
     2041 ctcgaagaca cggtggagct gcaatgcgcg gcccgtgacc acgtgccgct gcgcacgcgc
     2101 gcgggcgtgg tgtccatgac cgagctggtg c
//
LOCUS       DAOMVO010000389         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15329_length_2124_cov_15.599807, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000389 DAOMVO010000000
VERSION     DAOMVO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15329_length_2124_cov_15.599807"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKV30_13180"
     CDS             complement(<1..784)
                     /locus_tag="PKV30_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRL31204.1"
                     /translation="MSTPAEQQDLQQHGLYDPAHEHDACGVGFVAHIKGEKSHAIVTQ
                     ALKILENLDHRGAVGADKLMGDGAGILIQMPDKLYREEMAKQGVKLPPPGEYGVGMIF
                     LPKEHASRLACEEELERAIKLEGQVLLGWRDVPVNRDMPMSPTVRAKEPIIRQVFIGR
                     GHDVIVQDALERKLYVIRKTASAHIQALGLRYSKEYYVPSMSSRTVIYKGLLLADQVG
                     TYYRDLEDERCVSALGLVHQRFSTNTFPEWPLAHPYRYVAHNG"
     gene            complement(964..1668)
                     /locus_tag="PKV30_13185"
     CDS             complement(964..1668)
                     /locus_tag="PKV30_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRL31205.1"
                     /translation="MSRQPRLTVPGYPHHIIQRGNNRQPIFVDDADRQRLLGDLAEHA
                     RNRGVAVHAYVLMSNHLHLLVTPEAADSVPLMMQGVGRAYVRYFNRRHGRSGTLWEGR
                     YRSTLIEAERHLLACMVYIDLNPVRAGMLASAADYPWSSHAHLAGLRHDPLLTPHPLY
                     WQLGNTPFAREQAYVDLVRAGLAPEQHAALGQAAHSGWALGGDDFIADLEKRTARRAS
                     RAARGRPAKAEETASN"
     gene            1813..>2124
                     /locus_tag="PKV30_13190"
     CDS             1813..>2124
                     /locus_tag="PKV30_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31206.1"
                     /translation="MTKVTAGAQPPPRMAFAVAQSRRHFCHGPEEGTINTQDIWNFIT
                     TQGVDFGIKLLTAIVAWIIGRWLINLAVGLIGKGFERGGKIDATLAHYLKSIIAIVLN
                     IV"
BASE COUNT          383 a          664 c          670 g          407 t
ORIGIN      
        1 ctccgttgtg cgccacgtag cggtacgggt gcgccagcgg ccattcgggg aaggtattgg
       61 tggaaaagcg ctggtgcacc aggcccagtg ccgagacgca gcgctcgtct tccagatcac
      121 ggtagtaggt gccaacttgg tcggccagca gcagcccctt gtagatcacc gtgcggctgc
      181 tcatcgaggg cacgtaatac tctttgctgt agcgcaggcc gagcgcctgg atgtgggcgc
      241 tggcggtctt gcggatcaca tacagcttgc gctccagcgc gtcctgcacg atcacgtcgt
      301 gcccgcggcc aatgaacacc tggcggatga tcggctcctt ggcgcgcacg gtgggcgaca
      361 tgggcatgtc gcggttcacc ggcacgtcgc gccagcccag cagcacctgg ccttcgagct
      421 tgatggcgcg ctccaactct tcctcgcaag ccagacgcga cgcgtgctcc ttcggcaaaa
      481 agatcatgcc gacgccgtat tcgccgggcg gcggcagctt gacgccttgc ttggccatct
      541 cctcgcggta cagcttgtcg ggcatctgga tcaggatgcc cgcgccgtcg cccatcagct
      601 tgtcggcccc cacggcgccg cggtggtcga ggttttccag aatcttgagc gcctgggtga
      661 cgattgcgtg gcttttttcg cccttgatgt gcgccacaaa gccgacgccg cacgcgtcgt
      721 gctcgtgcgc cgggtcgtac aggccgtgtt gctgcaggtc ttgttgttca gcaggcgtgc
      781 tcatcgctca aatccattca tcgtcaaatc gcgggaagcg cgagcatagt gcagtgcaac
      841 atcgatgcca agcactttaa ttagggtcag atcaaaatta aaatcttgct tgatttatgc
      901 aggattaaat ggggacatat caaagttgat agctatttgc gctggatgga caagcgctaa
      961 cagtcaattt gatgcagttt cttcggcctt cgccggccgc ccacgcgcgg cgcgcgaagc
     1021 ccggcgggcg gtgcgctttt ccaggtcggc gatgaaatca tcaccaccca gcgcccaacc
     1081 gctgtgcgcg gcttgcccca gcgcggcgtg ctgctcgggt gccaggcccg cgcgcaccag
     1141 atcgacatag gcctgctcgc gcgcgaacgg cgtgttgccg agttgccagt acagcgggtg
     1201 cggggtcagc agcgggtcgt ggcgcagccc ggccaggtgc gcgtggctcg accaggggta
     1261 atcggctgcc gacgccagca tgccggcgcg cacgggattg aggtcgatgt agaccatgca
     1321 ggccagcaga tggcgctcgg cctcgatcag cgtgctgcgg tagcgccctt cccacagcgt
     1381 gccgctgcgc ccgtgccggc ggttgaagta gcgcacatag gcacgaccca cgccctgcat
     1441 catcagcggc acgctgtcgg ccgcctcggg cgtgacgagc aggtgaaggt ggttgctcat
     1501 cagcacatag gcgtgcaccg ccacgccgcg attgcgcgcg tgctcggcca gatcacccag
     1561 cagccgctgc cgatcggcgt cgtcgacgaa gatcggctgc cgattgttgc cgcgctggat
     1621 gatgtggtgc gggtagccgg ggacggtgag gcggggctgg cgggacatga attaatttta
     1681 atctgacccc aattaatatc aattaatttt aatctgaccc caattgagaa atcaagccaa
     1741 aacggctcca gcgcccgtcc agcaagcgcg agcagctatt gtttgtgcag caatccgaaa
     1801 ccccagatcc gcgtgaccaa agtcacggcc ggcgcgcaac cgccgcccag gatggctttt
     1861 gccgtggcac agtcgcgccg tcatttttgt cacggcccgg aggagggcac catcaacacc
     1921 caagacatct ggaatttcat caccacgcag ggcgtggatt tcggcatcaa gctgctgacg
     1981 gccatcgtcg cctggatcat cggccgctgg ctgatcaacc tggccgtggg cctgatcggc
     2041 aagggcttcg agcgcggcgg caagatcgac gccacgctgg cccattacct caagtcgatc
     2101 atcgccatcg tgctgaacat cgtc
//
LOCUS       DAOMVO010000390         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15330_length_2124_cov_10.729338, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000390 DAOMVO010000000
VERSION     DAOMVO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15330_length_2124_cov_10.729338"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKV30_13195"
     CDS             <1..449
                     /locus_tag="PKV30_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL31207.1"
                     /translation="TPGAASAAPAPAPVAAPPATPPSAGADDVDFIWPASGPMIAGFD
                     EAKNKGLGIAGKAGDPVLAAADGKVVYAGAGLRGYGNLIILKHNNTFLTAYAHNQTLL
                     VKEDQNVKRGQKIAEMGSTDADRVKLHFEIRRSGKPVDPARYLPAR"
     gene            695..>2124
                     /gene="rlmD"
                     /locus_tag="PKV30_13200"
     CDS             695..>2124
                     /gene="rlmD"
                     /locus_tag="PKV30_13200"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRL31208.1"
                     /translation="MNPESETVATMPPDWLHIESMDLDAQGIARRADGKVVFIDGALP
                     DEWVSAQVTRRKNNWEAATLTELHTESNLRVAPRCPHFGLHQGACGGCKMQHLEPTAQ
                     IAVKQRALEDNLWHLGKVKPELILRPIEGPAWGYRYRARLSVRYVQKKGEVLIGFHER
                     KSRYVADMRECHVLPPHVSSLLMPLRELIMGLQARETCPQIELAAGDDVTALVLRHLE
                     PLSHADRQQLRAFGQRHGVQWWLQPKGPDTVHLLDQDGPPLAYALHEFGITMPFKPTD
                     FTQVNPHINRVLVARALRLLGAKRDERVIDWFCGLGNFTLPIATQAGQVLGVEGSAAM
                     VARARQNFESNRAEALEAKALAATDFAARNLFDMTAEQLIADGSADRWLVDPPREGGF
                     ALVKALAAIHQARIGAEGADPLPLGAETWQPPRRIVYVSCNPATLARDAGLLVHQAGY
                     RCTAAGVVNMFPHTAHVESMAVFDRA"
BASE COUNT          380 a          723 c          700 g          321 t
ORIGIN      
        1 ccacgcccgg cgccgccagc gcggcccccg cgccagcgcc cgtggctgcg ccacccgcca
       61 cgccgccatc ggccggcgcc gacgatgtcg acttcatctg gcccgccagc ggccccatga
      121 tcgccggctt cgacgaagcc aagaacaagg gcctgggcat tgccggcaag gccggcgacc
      181 cggtgctggc cgccgccgat ggcaaggtgg tatacgccgg cgccggcctg cgcggctatg
      241 gcaacctgat catcctcaag cacaacaaca ccttcttgac ggcatacgcg cacaaccaga
      301 cgctgctggt gaaggaagac cagaatgtca agcgcggcca gaagatcgcc gagatgggct
      361 cgaccgacgc cgatcgggtc aagctgcact tcgagatccg ccgctcgggc aagccggtgg
      421 atccggcacg gtatctacct gcgagataaa gctccccctg agtcgccttc ggcgccatcc
      481 ccctgagggg aacaacgctg gtggtcgggg ggaccccgac cacggcgttc cagcgtggcc
      541 tgctccgcgg ccttttgaat ggcttgctgc gcaccctgag ggctccctct cccgctggcg
      601 ggagagggtt ggggtgaggg cggtggcgca tcaagaacta tgcaattgat agctgctcgc
      661 gcttgtccat aaagcgccag cggccttttt catcatgaac ccagagtctg aaaccgtcgc
      721 caccatgccg cccgactggc tgcacatcga gtcgatggac ctcgacgcgc aaggcatcgc
      781 gcgccgtgcg gacggcaagg tggtgttcat cgatggcgcg ctgccggacg aatgggtcag
      841 cgcccaggtc acgcggcgca agaacaactg ggaagcggcc acgctgaccg aattgcacac
      901 cgaaagcaat ctgcgcgtgg cgccgcgctg tccgcacttc ggcctgcacc agggcgcctg
      961 cggcggctgc aagatgcagc acctggagcc gacggcgcaa atcgccgtca agcagcgcgc
     1021 actggaagac aacctgtggc acttgggcaa ggtcaagccc gagttgattc tgcgccccat
     1081 tgaaggcccg gcctggggtt accgctaccg cgcgcggctg tcggtgcgct acgtgcagaa
     1141 aaagggcgag gtgctgatcg gttttcacga acgcaagagc cgctacgtgg ccgacatgcg
     1201 cgaatgccac gtgctgccgc cgcacgtgag cagcctgctg atgccgctgc gcgaactcat
     1261 catgggcctg caggcgcgcg aaacctgccc gcagatcgaa ctggccgccg gcgatgacgt
     1321 gaccgcgctg gtgctgcgcc acctggagcc gctcagccac gccgaccgtc agcaactgcg
     1381 cgccttcggc caacgccacg gcgtgcaatg gtggttgcag cccaaggggc cggacacggt
     1441 gcatttgctg gatcaggatg gcccgccgct ggcctacgcg ctgcacgagt tcggcatcac
     1501 catgccattc aagccgaccg atttcacgca ggtcaacccg cacatcaacc gcgtgctggt
     1561 ggcccgcgcg ctgcgcctgc tcggcgcgaa acgggacgag cgcgtaatcg actggttctg
     1621 cggtctcggc aacttcacgc tgccgatcgc cacacaggca gggcaggtgc tgggagtgga
     1681 aggcagcgcc gcgatggtgg cgcgcgcgcg tcagaatttt gagtcaaatc gggctgaagc
     1741 gcttgaggcg aaagcgctgg cagctaccga tttcgcagcg cgcaacctgt tcgacatgac
     1801 cgccgaacaa cttatcgccg acggctcggc cgaccgctgg ctggtcgatc caccgcgcga
     1861 aggtgggttc gcgctggtca aggcgctggc cgcgatccac caggcgcgca tcggcgccga
     1921 gggtgccgat ccactgccgc tcggtgccga aacctggcag ccaccgcgcc gcatcgtcta
     1981 cgtcagctgc aaccccgcca cattggcgcg cgacgccggt ctgctggtgc accaagccgg
     2041 ctatcgctgc acggcggcgg gcgtggtcaa catgttcccg cacacggcac acgtggagag
     2101 catggcggtg tttgatcgcg cgga
//
LOCUS       DAOMVO010000391         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15438_length_2113_cov_13.750729, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000391 DAOMVO010000000
VERSION     DAOMVO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15438_length_2113_cov_13.750729"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKV30_13205"
     CDS             complement(<1..376)
                     /locus_tag="PKV30_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL31209.1"
                     /translation="MNEYKILFTGTMGAGKTTAIGAVSETPPIVTDVVNNDQSHTKER
                     TTVGLDFGQFTLDSGDRIRMFGTPGQSRFDFLWKILSKNALGMIILTDNSRPDPLGDL
                     RVYLEGFADDLDIMPCAIGIGRL"
     gene            complement(398..898)
                     /locus_tag="PKV30_13210"
     CDS             complement(398..898)
                     /locus_tag="PKV30_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31210.1"
                     /translation="MNSGRAPNTLERPIRADRLQDWLKNTEHDLIQTRPATLLDEASL
                     PTAAEAPAAAPVQINPLRFKLRRWPPAALLRSDPNRIRMATLLSRRALNAAELATISQ
                     QPVQECQAFLTTLQTTGLVDVQKAPVAPPAGSAPAQAAAAPKPAAPAPAFARGLISGI
                     RRRLGL"
     gene            1122..>2113
                     /locus_tag="PKV30_13215"
     CDS             1122..>2113
                     /locus_tag="PKV30_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRL31211.1"
                     /translation="MFVHLRLHTEFSVVDGTTRVDDVVAAAARDRQPALAITDFGNLF
                     GAIKFYKAARGAGVKPLIGAEVLLEGREAEQDAPSRVLLLVVNHQGYLNLSELLARAW
                     TQGVVKAQAVVRWAWLAELADGLILLSGAQAGPVGQALLQGQTQQASDAALKLAELFP
                     HRFYLELQRAGRRDDEAHVAAAVQLAARLKLPVVATHPVQFATPDDYEAHEARVCIAE
                     GEILGNPRRVRRFTPEQYFKSSAEMAELFADLPSAVANAAEIAKRCNLTLVLGKPQLP
                     EFPVPDGHTIETYFRQASLDGLEERLQHLYPDAAKRDAERPRYLERLEFEIGTIL"
BASE COUNT          322 a          659 c          768 g          364 t
ORIGIN      
        1 ccaggcggcc gatgccgatg gcgcagggca tgatgtccag gtcgtcggca aagccttcga
       61 ggtacacgcg caggtcgccc agcgggtcgg ggcgcgagtt gtcggtcagg atgatcatgc
      121 cgagcgcgtt cttcgacagg atcttccaga ggaagtcgaa gcgcgactgg cccggcgtgc
      181 cgaacatgcg gatgcggtcg ccgctgtcca gcgtgaactg gccgaagtcc aggccgacgg
      241 tggtgcgttc cttggtgtgc gactggtcgt tgttgacgac gtcggtgacg atgggcggtg
      301 tctcgctgac ggcgccaatg gcggtcgtct tgcccgcgcc catggtgccg gtgaaaagga
      361 tcttgtattc gttcatggtg gctgcgaacg gcttaattta caggccgagc cggcgacgga
      421 tgccggaaat gagaccgcgc gcgaacgcgg gcgcgggggc ggcgggtttg ggtgcggcag
      481 cggcttgcgc gggtgcactg ccagcgggcg gtgcgaccgg cgccttctga acatcgacca
      541 ggccggtggt ctgcagcgtg gtcaggaagg cctggcattc ctgcactggc tgctggctga
      601 tggtggccag ctcggccgcg ttcaacgcgc ggcgcgacag cagcgtggcc atgcggatgc
      661 ggttggggtc gctgcgcagc agcgcggccg gcggccagcg gcgcagcttg aagcgcagcg
      721 ggttgatctg cactggtgcg gcggcgggtg cttcggccgc ggtgggcaag ctggcttcat
      781 ccagtagcgt ggccgggcgg gtctgaatca ggtcgtgctc ggtgtttttc agccagtcct
      841 gcagccggtc ggcgcggatc gggcgctcga gcgtgtttgg cgcacggccc gaattcatgc
      901 gcgtgagccg cagtatctgg gcgactgctg gaaagaagta tatttttggc ttcaccgccg
      961 cctgcacgtc tgcccgcgca atatgcgcac taatgcgcag aagcctgtcg taagctgcga
     1021 agcgtcgtga ttgctgcacg acaactaatt cacacccatg ttgcaccagc tgctgccaat
     1081 gaagcgtggc acgccttgca acttcttggt agcgttatcc gatgtttgtc catttgcgtt
     1141 tgcacaccga gttttcagtc gtcgacggca ccacgcgcgt ggacgacgtg gtggcggcag
     1201 cagcgcgcga ccggcagccg gcactggcca tcaccgattt cggcaacctg tttggcgcca
     1261 tcaagttcta caaggccgcg cgcggcgccg gcgtcaagcc gctgatcggt gccgaggtgc
     1321 tgctggaggg gcgcgaggcg gagcaggacg cgcccagccg cgtgctgctg ctggtggtca
     1381 accaccaggg ctacctgaac ctgtccgagc tgctggcccg cgcgtggacg caaggcgtgg
     1441 tcaaggcgca agcggtggtg cgctgggcct ggctggccga gctggccgac gggctgatcc
     1501 tgctgtcggg tgcgcaggcg gggccggtcg ggcaggcgct gctgcaaggg cagacgcagc
     1561 aggcgagcga tgcggcgctc aagctggccg agctgtttcc gcaccgcttc tacctggagc
     1621 tgcagcgcgc cggccgccgc gacgacgagg cgcacgtggc cgccgccgtg caactggccg
     1681 cgcgcctgaa gttgcccgtg gtggccacgc acccggtgca gttcgccacc ccggacgact
     1741 acgaagccca cgaggcgcgc gtgtgcatcg ccgagggcga aatcctgggc aacccgcgcc
     1801 gcgtgcgccg cttcacgccc gagcagtact tcaaatcatc ggccgagatg gccgagcttt
     1861 ttgccgacct gccctcggcg gtggccaacg cggctgaaat cgccaagcgc tgcaacctga
     1921 cgctggtgct gggcaagccg cagttgccgg agtttccggt gcctgatggc cacaccatcg
     1981 agacctactt ccgccaggcc tcgctggacg ggctggaaga gcgcctgcag cacctgtacc
     2041 ccgacgcggc caagcgcgac gccgagcgcc cgcgctacct ggagcggctg gagttcgaga
     2101 tcggcaccat cct
//
LOCUS       DAOMVO010000392         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15500_length_2108_cov_15.027277, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000392 DAOMVO010000000
VERSION     DAOMVO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15500_length_2108_cov_15.027277"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKV30_13220"
     CDS             complement(<1..544)
                     /locus_tag="PKV30_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL31212.1"
                     /translation="MAQYVFSMNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGIDKDIEGEAIPMPGLDIGYLPQEPQLNPEHTVREAVQEAMKEVNDARA
                     RLEEIYAAYAEEDADFDALSEEQGKLEAIIAAAGTDSEHQLEIAADALRLPPWDAVIG
                     KLSGGEKRRVALCKLLLSKPD"
     gene            complement(726..1304)
                     /locus_tag="PKV30_13225"
     CDS             complement(726..1304)
                     /locus_tag="PKV30_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31213.1"
                     /translation="MAVSTTFGYNAPEDRIWLACAAWPERLWLTRRMVRAVLQAAVSA
                     LEADPPALPSGADARPAAERAAAEHDASLNQPQPGEQGRALQMGREANDAPALQGAVL
                     CTRFSLMTLGTQTELVFDTPAGQRRLRLSRIGLHRWLHALHMVLRSADWADWPAAPDW
                     LTRSYLPPALQSLLVVPPADLTLNDEQDGDAP"
     gene            1523..>2108
                     /locus_tag="PKV30_13230"
     CDS             1523..>2108
                     /locus_tag="PKV30_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL31214.1"
                     /translation="MNFDELNLAPAILRAVQEQGYTAPTPIQAQAIPVVLGGHDLLAG
                     AQTGTGKTAGFTLPMLQLLSTRPAAKAGAIRALVLTPTRELAAQVEEAIRAYGQYLEL
                     TSTVIFGGVGMNPQINRIARGVDILVATPGRLLDLEGQNHIDLSHVEILVLDEADRML
                     DMGFIHDVKKVLALLPRDKQSLLFSATFSDEIREL"
BASE COUNT          334 a          739 c          704 g          331 t
ORIGIN      
        1 tgtcgggctt ggacagcagc agcttgcaca gcgccacgcg gcgcttctcg ccgcccgaga
       61 gcttgccgat caccgcgtcc cacggcggca ggcgcagcgc gtcggcggcg atctcgagct
      121 ggtgctcgct gtcggtgccg gcggcggcga tgatcgcttc gagcttgccc tgctcctcgc
      181 tcagcgcgtc gaagtcggca tcctcctcgg cgtaggcggc gtagatttcc tccagccggg
      241 cgcgggcgtc gttgacttcc ttcatcgcct cttgcaccgc ctcgcgcacg gtgtgctcgg
      301 ggttgagctg cggctcctgc ggcaggtagc cgatgtccag gccgggcatg ggaatggcct
      361 cgccctcgat gtccttgtcg atgccggcca tgatcttcag cagcgtggac ttgcccgagc
      421 cgttcaggcc cagcacgccg atcttggcgc cgggaaagaa ggacagcgaa atatccttca
      481 ggatctgccg cttgggcggc acgatcttgc cgacgcggtt catggaaaaa acgtactggg
      541 ccatggattt actctcaaaa aaatagctgc tcgcgcttga cctacaagcg ccagaggcca
      601 atttgattca aaaacaggat tggggatggc gggccatcgc gcggcggggc cgcgggccgg
      661 tcagggggga ttatcggtgt tgggcgcaag ccggctggcg cgcacgggca tgcgcgcggc
      721 gcggctcacg gggcgtcgcc gtcctgctcg tcgttcagcg tcagatcggc cgggggcacc
      781 accagcagcg actgcagcgc cggcggcagg tagctgcgcg tgagccaatc gggcgccgcc
      841 ggccagtcgg cccagtcggc cgagcgcagc accatgtgca gcgcgtgcag ccagcggtgc
      901 aggccgatgc ggctcaagcg caggcgccgc tgcccggccg gcgtgtcgaa caccagttcg
      961 gtctgcgtgc ccagagtcat caagctgaag cgcgtgcaca gcacggcgcc ctgcagcgcg
     1021 ggcgcgtcgt tcgcttcacg ccccatctgc agcgcccgcc cctgctcgcc cggttgcggc
     1081 tggttgagcg aggcgtcgtg ctcggccgcc gcgcgctcgg cggccggccg cgcgtcggcg
     1141 ccgcttggca gcgcgggcgg gtccgcttca agcgccgaca ccgccgcctg cagcacggca
     1201 cgcaccatgc gccgcgtcag ccacagccgc tcgggccagg cggcgcaggc cagccagatg
     1261 cggtcttcgg gggcgttgta gccgaaggtg gttgaaactg ccatgggagc tgaaaacttc
     1321 acttggactt ggggcatgcg ataatcaccc cgcttgccgc ccaccagtat gctgcaagcc
     1381 agcccttccg ctggggcgtt gccggccaca tcacaggccc gcgcccactt caagaagaac
     1441 ccgctgcagc cttcacaact ggccggcacc cgccggaaca ggccagcagc tccccgcgcc
     1501 gagagaccgg tgcatccatt tcatgaactt tgacgaactg aacctggccc ccgccatcct
     1561 gcgcgcggtg caagagcagg gctacacggc ccccaccccc atccaggcgc aagccattcc
     1621 cgtggtgctg ggtggccacg acctgctggc cggcgcacag accggcaccg gcaagacggc
     1681 cggcttcacg ctgcccatgc tgcagctgct cagcacgcgc ccggcagcca aagcgggcgc
     1741 catccgcgcg ctggtgctga cgcccacgcg cgagctggcg gcgcaggtcg aggaagccat
     1801 tcgcgcctac ggccaatacc ttgagctgac ttccaccgtc atcttcggcg gcgtcggcat
     1861 gaacccgcag atcaaccgca tcgcgcgcgg cgtcgacatt ctggtcgcca cgccgggccg
     1921 tctgctcgat ctggaaggcc agaaccacat cgacctctcg cacgtggaaa ttctggtgct
     1981 ggacgaggcc gaccgcatgc tcgacatggg cttcatccac gacgtgaaga aggtgctggc
     2041 gctgctgccc agggacaagc agagcctgct gttctcggcc accttcagcg acgagatccg
     2101 cgagctgg
//
LOCUS       DAOMVO010000393         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15534_length_2105_cov_10.887805, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000393 DAOMVO010000000
VERSION     DAOMVO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15534_length_2105_cov_10.887805"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2105
                     /locus_tag="PKV30_13235"
     CDS             <1..>2105
                     /locus_tag="PKV30_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRL31215.1"
                     /translation="QREAAALLAQRIELLSTKPWRDAEAQQPGLQADLDQWRAHARLL
                     LADPQWLSVDVRYPPQLEAAQSQLAAVWDAFGAALAQVVAAAQDAAAPLPPVPVWADE
                     IRAARGETPAAAPAADKPAKPKIDPEQRASAQKAVEAGTMLLEQAVAAGHTKNTHSAT
                     QALRQSLKAHGRLIDDALEARVHAALVSAGELEGWQRWSADKVREQLVARAEALLVRR
                     KGKGGVAPVDEAANAEANQATAVTEQAGAMEQAQVATENEATRADQTSAGAENNAESV
                     DDASATAANAEANVPTAVTEQAGAAQDAAAAATRAAPAADDEMVPAMGGRKLQETIRK
                     LREEWKAADQGGAPNHALWKRFDRAVNAAHQHVDAWLAKVRAEAAEHRAQRLALIDEV
                     KAFGAAQAAQGEGADWKAFNRQIHQFSDRWRNAGHLSEKQFAELQPQWKDAIHAAAAP
                     LEAAQKQSTARRQALIQEAEQLGAAPQLRVDAVRALQQRWQAEAQAVPLDRKHEQKLW
                     DAFRKPLDDAFNRKGAEREKQAAAMSAHDKAVLDAAKALEAANASGDAQRIRAAMAQL
                     EAATRGELPKAAAEAPASAPGSEEKAAPAPEGAAPAAPENEAIDTAAAAPEGDAAGDD
                     EGPAAAEAPAPAAPPKPAKPLVAMRGDDRPGAKRAEPAAAGRGGKFGDRPGARPGGRD
                     ARGGPGGRFGDRPPREDRGP"
BASE COUNT          383 a          804 c          723 g          195 t
ORIGIN      
        1 cagcgcgaag ccgccgccct gctggcgcag cgcatcgagc tgctgtccac caagccctgg
       61 cgggacgccg aggcgcagca gcccggtctg caggccgacc ttgaccaatg gcgcgcccac
      121 gcccgcctgc tgctggccga cccgcagtgg ctgagcgtgg acgtgcgcta cccgccgcag
      181 ctggaagccg cgcaaagcca gctcgccgcc gtgtgggatg cctttggcgc cgcgctggcc
      241 caggtagtgg ccgccgcgca agacgccgcc gcgccgctgc cgcccgtgcc cgtgtgggcc
      301 gacgagatcc gcgccgcgcg cggcgaaaca cccgccgctg cgcccgctgc tgacaagccg
      361 gccaagccga agatcgaccc cgagcagcgc gcatccgcgc aaaaggcggt cgaagccggc
      421 accatgctgc tggagcaggc cgtcgccgcc ggccacacca agaacaccca cagcgccacc
      481 caggcgctgc gccagtcgct caaagcccat ggccgcctga tcgacgacgc gctcgaagcg
      541 cgcgtgcacg ccgcgctggt gagtgccggc gagctggaag gctggcagcg ctggagcgcc
      601 gacaaggtgc gcgagcagct cgtcgcccgc gctgaagcgc tgctggtgcg ccgcaagggc
      661 aagggcggcg tcgcgccggt tgatgaagct gccaacgccg aagccaatca agccaccgcc
      721 gtcaccgaac aggctggggc gatggagcag gctcaggttg ctacagaaaa cgaagctacc
      781 cgcgctgatc agacaagcgc tggagccgaa aacaatgctg aatccgtaga tgatgccagc
      841 gccacggccg ccaacgccga agccaacgtg ccgacggccg tcaccgaaca ggctggcgcg
      901 gcccaagacg ctgcggctgc cgccacccgc gccgcgcccg ctgccgacga cgagatggtg
      961 cccgccatgg gcggccgcaa gttgcaggaa accatccgca agctgcgcga agaatggaag
     1021 gccgccgacc agggcggcgc gcccaaccac gcgctgtgga agcgctttga ccgcgccgtc
     1081 aacgccgcgc accagcacgt cgatgcctgg ctggccaagg tgcgcgccga agccgccgaa
     1141 caccgcgccc agcgcctggc gctgatcgac gaggtcaagg cctttggcgc cgcccaggcc
     1201 gcgcaaggcg agggcgccga ctggaaggcc ttcaaccgcc agatccatca gttcagcgac
     1261 cgctggcgca acgccggcca cctgtctgaa aagcagtttg ccgagctgca gccgcagtgg
     1321 aaggacgcca tccacgccgc cgccgccccg ctcgaagccg cgcaaaagca aagcaccgcg
     1381 cgccgccaag cgctgatcca ggaagccgaa caactcggcg ccgcgccgca gctgcgtgtg
     1441 gacgccgtgc gcgccctgca gcagcgctgg caggccgagg cgcaggccgt gccgctcgac
     1501 cgcaagcatg agcaaaagct gtgggacgcc ttccgcaagc cgctggacga cgccttcaac
     1561 cgcaagggcg ctgagcgcga aaagcaggcc gccgccatga gcgcgcacga caaggccgtg
     1621 ctcgacgccg ccaaggcgct ggaagccgcc aacgccagcg gcgacgcgca gcgcatccgc
     1681 gccgccatgg cgcagctgga agcggccacg cgcggcgagt tgcccaaagc cgcggccgag
     1741 gcacccgcca gcgcaccggg atcggaagaa aaagcggcgc cagcgcccga gggcgccgcg
     1801 ccggcagctc ctgaaaacga agcaatcgat accgcagctg cagcgcccga aggcgatgca
     1861 gccggcgatg acgaaggccc cgctgccgcc gaagcgcccg cgcctgcggc accgcccaag
     1921 cccgccaaac cgctcgtcgc catgcgcggc gacgaccgcc ccggcgccaa gcgcgctgaa
     1981 cccgctgcag ccggccgtgg cggcaagttc ggcgaccgcc cgggtgcacg tccgggtggc
     2041 cgcgacgcgc gcggcggccc cggtggccgc tttggcgacc gcccgccgcg cgaagaccgt
     2101 ggccc
//
LOCUS       DAOMVO010000394         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15557_length_2103_cov_12.813965, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000394 DAOMVO010000000
VERSION     DAOMVO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15557_length_2103_cov_12.813965"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /gene="pobA"
                     /locus_tag="PKV30_13240"
     CDS             <1..1196
                     /gene="pobA"
                     /locus_tag="PKV30_13240"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HRL31216.1"
                     /translation="TRTQVAIVGAGPSGLLLGQLLHKAGIDAVIVERVSGEYVLGRIR
                     AGILEQVCIDLMDEAGVGARMHKEGLVHDGFEMLFGGQRHRVDLKKYSGGKHVMVYGQ
                     TELTHDLMDARAAAGLTTIYEARNAAVHDFDTAKPYVTYEKDGQQHRIDCDFIAGCDG
                     FHGVCRASAPRSAIKEYEKVYPFGWLGLLSDTPPVNHELVYVNSPRGFALCSQRSNTR
                     SRYYLQVPLTDKVEEWSDDAFWQELKLRLDDEAREKIVTGPSIEKSIAPLRSFVTEPM
                     RFGRMFLAGDAAHIVPPTGAKGLNLAATDVKYLFDALVGFYQDKSEAGLDDYSSKALA
                     RIWRAERFSWWFTQLMHRFPHDEAITAKFQNAELDYLMHSEAGLTSIAENYVGLPLDF
                     GARRA"
     gene            1305..>2103
                     /locus_tag="PKV30_13245"
     CDS             1305..>2103
                     /locus_tag="PKV30_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRL31217.1"
                     /translation="MATASSPQPLRGTRIVSLALNLPGPAALMRLAAMGARCLKVEPP
                     GPGTTSAGRPISGDPMGQYDIGAYSALHEGIRVVQLDLKAERGQAQLARELLRADLLL
                     TSFRPSALVKLGLDWKALRRTCPHLSMVEIVGAPGARADEPGHDLTYVAEAGLVTGTE
                     LPPTLLADMAGALMASEAALAALMQARATGRGQHRQVALSSAAEWLALPRTWGLTLPS
                     GLVGGAHAGYRVYPCKDGRVAIAALEPHFARALFAVAGLPPPDARAPF"
BASE COUNT          349 a          745 c          691 g          318 t
ORIGIN      
        1 ccacccgcac ccaggtcgcc atcgtcggcg ccggcccttc gggcctgctg ctgggccagt
       61 tgctgcacaa ggccggcatc gacgccgtga tcgtcgagcg cgtcagcggc gaatacgtgc
      121 tcggccgcat ccgcgccggc atcctggagc aggtctgcat cgatttgatg gacgaagccg
      181 gcgtgggcgc gcgcatgcac aaggaaggcc tggtgcacga cggcttcgag atgctgtttg
      241 gcggccagcg ccaccgcgtc gatctgaaga aatactccgg cggcaagcac gtgatggtct
      301 acggccagac cgagctgacg cacgatttga tggacgcgcg cgcggctgcc ggcttgacga
      361 ccatctacga ggcgcgcaac gctgccgtgc acgacttcga caccgccaag ccttacgtca
      421 cgtacgagaa agacggccag cagcaccgca tcgactgcga cttcatcgcc ggctgcgacg
      481 gctttcacgg cgtgtgccgc gccagcgcgc cacgcagcgc gatcaaggaa tacgaaaagg
      541 tctacccctt cggctggctc ggcctgctgt ccgacacgcc gccggtgaac cacgaactcg
      601 tctacgtcaa cagcccgcgc ggctttgcgc tgtgcagtca gcgcagcaac acgcgcagcc
      661 gctattacct gcaggtgccg ctgaccgaca aggtggaaga atggagcgac gacgccttct
      721 ggcaggagct gaagctgcgt ctggacgacg aggcgcgcga aaaaatcgtc accggcccca
      781 gcatcgaaaa aagcatcgcc ccgctgcgca gcttcgtcac cgagccgatg cgttttggcc
      841 gcatgttcct ggccggcgac gcagcgcaca tcgtgccgcc caccggcgcc aaagggctca
      901 acctggcggc gaccgacgtg aagtacctgt ttgacgcact ggtcggcttc taccaggaca
      961 agagcgaagc ggggctcgac gactactcga gcaaggccct ggcccgcatc tggcgcgccg
     1021 agcgcttttc gtggtggttc acgcagctca tgcaccgctt tccgcacgac gaagcgatca
     1081 ccgccaagtt ccagaacgcc gagctggatt acctgatgca ctcggaagcc ggcctgacca
     1141 gcatcgccga gaactacgtc ggcctgccgc tcgacttcgg cgcgcggcgc gcttaagcgc
     1201 aagccagcgc cccaagggcc tggcaggcgg tgaatcggct ataaattggc ttcccgcgct
     1261 tgcccagcaa gcgtcaccag ctatattttc aatagtccac tgccatggcc actgcctcat
     1321 cgcctcagcc gctgcgcggc acccgcatcg tcagcctggc gctcaacctg cccggcccgg
     1381 ccgcgctgat gcgcctggcc gccatgggcg cgcgctgcct gaaggtggag ccgcccggcc
     1441 ccggcaccac gtcggccggc cggccgatca gcggtgaccc gatgggccag tacgacatcg
     1501 gtgcctacag cgcgctgcac gaaggcatcc gcgtggtgca actcgatctg aaagccgagc
     1561 gcgggcaggc gcaactggcg cgcgaactgc tgcgcgccga cttgctgctc acctcatttc
     1621 gcccctcggc gctggtcaag ctggggctgg actggaaggc gctgcgccgc acctgtccgc
     1681 acctgtcgat ggtcgaaatc gtcggcgcac cgggcgcgcg tgccgacgag ccggggcacg
     1741 acctgaccta tgtggccgag gccggactgg tcaccggcac cgagttgccg cccaccctgc
     1801 tggccgacat ggctggcgcg ttgatggcca gcgaggccgc gctggcggcg ctgatgcagg
     1861 cccgcgccac gggccgtggc cagcaccggc aggtggcgct gtcgagcgcg gccgaatggc
     1921 tggcgctgcc gcgcacatgg ggcctgacgc tgcccagcgg tttggtcggc ggcgcgcacg
     1981 ccggctaccg cgtgtacccg tgcaaggacg ggcgcgtcgc catcgccgcg ctggagccgc
     2041 actttgcgcg cgcgctgttc gccgtcgccg gattgccccc gcccgacgcg cgtgcaccgt
     2101 ttg
//
LOCUS       DAOMVO010000395         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15653_length_2094_cov_14.333006, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000395 DAOMVO010000000
VERSION     DAOMVO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15653_length_2094_cov_14.333006"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKV30_13250"
     CDS             complement(<1..1064)
                     /locus_tag="PKV30_13250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRL31218.1"
                     /translation="MQTCDVLIIGAGIAGSSLAWRLARGGLAVLLLERESQPGMHSTG
                     RSAAMFMESYGPPGVRALTRASRDFYLHPPPGFADQPLLAPRAALFVATTEQHASLAA
                     MQAELEATGTQLSLLNKEQLALVAPALKPDLFDQALLDVHGYDIDVNAMLQGFLRGAR
                     QAGARLLTGAVPTETTRTGDAWRVTLSNGDVVQARTVVDAAGAWADEVATLFGAAPIG
                     LEPRRRSAFTFRAPEGVDVSNWPMVADVDEGWYIKVDAGQLLGSPANADPVEPHDVQP
                     EELDIAMGIHAIQEATTLEIRRPTATWAGLRSFVRDGEIVIGFDDAVPQFFWLAAQGG
                     YGIQSAAGASLLAASLIEGCA"
     gene            complement(1136..>2094)
                     /locus_tag="PKV30_13255"
     CDS             complement(1136..>2094)
                     /locus_tag="PKV30_13255"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRL31219.1"
                     /translation="HRKHHAKCEQAEDPHSPQTHGLKKVMWQGAELYRAEARNPDTLS
                     RYGHGTPSDWMERHVYERHSVLGVSLMLIIDMALFGALGAAVWAVQMAWIPFWAAGVI
                     NGVGHFWGYRNFEAQDASTNVSPWGLIVGGEELHNNHHTYPTSAKFSVKPYEFDIGWM
                     YITVLNKLGLAQPKKTPPRLAYGAVRPVADDQTLEALIAHRYEVMASYARQMRAVFAQ
                     QTEAADASGALRSARLWLHRDAEKVPAAAVPQLAEARAAYPALDKMVTMREELRQLWL
                     NTGRTREQLVTDLQAWCHRAEESGIAALRDFSLKLRAVRVAA"
BASE COUNT          326 a          687 c          742 g          339 t
ORIGIN      
        1 gcgcagcctt cgatcaagct ggccgccagc agactggcgc cggcggcgct ctgaataccg
       61 tagccgccct gcgcggcgag ccagaaaaat tgcggcacgg catcgtcaaa gccgatgacg
      121 atttcgccat cgcgcacgaa gctgcgcagc ccggcccagg tggccgtggg gcggcgaatt
      181 tccagcgtcg tcgcttcctg gatcgcgtgg atgcccatgg cgatgtccag ttcttcgggc
      241 tgcacgtcgt gcggctccac cgggtcagcg ttggcgggcg agccgagcaa ctggccggca
      301 tcgactttga tgtaccagcc ttcgtcgaca tccgccacca tcggccagtt ggacacatcc
      361 acgccttcgg gcgcgcgaaa ggtgaaagcg ctgcggcggc gcggctccag gccgatgggc
      421 gcggcgccga acagcgtggc cacctcgtcg gcccaggcgc cggcggcatc gaccacggtg
      481 cgcgcctgca cgacatcgcc gttcgacagc gtgacgcgcc aggcgtcgcc cgtgcgcgtg
      541 gtctcagtgg gcacggcgcc cgtgagcagc cgtgcaccgg cctgccgcgc gccgcgcaga
      601 aagccttgca gcatggcgtt gacgtcgatg tcgtagccgt gcacgtccag cagggcttga
      661 tcaaacaaat cgggcttcag cgcaggtgca acaagcgcaa gctgctcttt attcagtagc
      721 gagagctgcg tgcccgtggc ttccagctcg gcctgcatgg cggccagcga ggcgtgctgc
      781 tcggttgtgg cgacgaacag cgcggcacgc ggcgccagca gcggctgatc ggcaaagcct
      841 ggcggcgggt gcaagtagaa gtcgcggctg gcgcgcgtca gcgcccgcac gccgggcggg
      901 ccgtagcttt ccatgaacat ggcggcggag cggccggtgc tgtgcatgcc gggctgggat
      961 tcgcgctcca gcagcagcac cgctagaccg ccgcgcgcca gccgccaggc cagggaactg
     1021 ccggcgatgc cggcgccgat gatgagcacg tcgcatgtct gcatgggcca ttctggcacg
     1081 cagggcacaa aaaagcccgg cgcgcggccg ggctgggtgc gaagtcgaaa gcgggtcagg
     1141 ccgccacgcg aactgctcgt aatttcagcg aaaagtcgcg cagagcggcg atgccgcttt
     1201 cttcggcgcg gtggcaccag gcttgcagat cggtcaccag ctgctcgcgc gtgcggccgg
     1261 tgttgagcca caactggcgc agttcctcgc gcatggtgac catcttgtcg agtgcggggt
     1321 aagccgctcg cgcctcggcc agttgaggca cggcggcggc gggcaccttt tcggcgtcgc
     1381 ggtgcaacca aaggcgggcc gagcgcagcg cgccgctggc gtcggcggcc tcggtctgct
     1441 gcgcgaacac ggcgcgcatc tggcgcgcgt aactggccat gacttcgtag cggtgggcga
     1501 tcagcgcctc cagcgtctgg tcgtcggcca ccgggcgcac ggcgccgtag gccaggcgcg
     1561 gcggcgtctt cttgggctgc gccaggccca gcttgttgag cacggtgatg tacatccagc
     1621 cgatgtcgaa ctcgtacggc ttgaccgaaa acttggccga cgtggggtag gtgtggtggt
     1681 tgttgtgcag ctcttcgccg cccacgatca agccccaggg cgagacattg gtggaagcat
     1741 cctgcgcctc gaagttgcgg taaccccaga agtggcccac gccgttgatg acgcccgcgg
     1801 cccagaacgg aatccacgcc atctgcacgg cccacaccgc ggcgcccaag gcgccgaaca
     1861 aggccatgtc gatgatcagc atcaggctca cgccgagcac cgaatggcgt tcgtacacgt
     1921 ggcgctccat ccagtcgctg ggcgtgccgt ggccgtagcg cgaaagcgtg tcggggttcc
     1981 tggcttcggc gcggtacagc tcggcgccct gccacatgac ttttttcagg ccgtgggttt
     2041 gcgggctgtg cgggtcttcg gcctgctcgc acttggcgtg gtgcttgcgg tgga
//
LOCUS       DAOMVO010000396         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15696_length_2090_cov_11.273710, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000396 DAOMVO010000000
VERSION     DAOMVO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15696_length_2090_cov_11.273710"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..505)
                     /locus_tag="PKV30_13260"
     CDS             complement(56..505)
                     /locus_tag="PKV30_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL31220.1"
                     /translation="MARIVFDLPAHFVFSTEMQIYISHVNQGGHLDNAQLLSLVSEAR
                     VRFFQAQGYPEADVGGLAIVVGDIVAQYKSEGFHGETMRVEMTPADFNPYGFDLVFRM
                     TEKSQGREVARGKTGIVFINKSDRKVALLPAHIRALLSRPPVSAAQP"
     gene            complement(595..>2090)
                     /gene="rpoN"
                     /locus_tag="PKV30_13265"
     CDS             complement(595..>2090)
                     /gene="rpoN"
                     /locus_tag="PKV30_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRL31221.1"
                     /translation="EVEQMLDENPFLERTAEEAPREEFGLEAADTPAQTDDYSADDAL
                     FSGAASASSTSAEGQNDAEAPSASADEPDWSGDGTVEMAPNDAEWGGDAPARNRNDSE
                     GDEADATELARSHESLTSFLHRQALALRLSEIDRAALRFLIESLNDDGYLEEPLEELA
                     TGLAGEGDLEQIDELVHRFTVAQRLLQSLEPVGVGARDLAECLTLQLKAMAADEDSAI
                     DPDTVQTALRVCQQPLEMLARRDIRRLTQLCGDGEERTRAAMALIARLEPRPGRRFVD
                     VERNIIVPDVIVRKAGREGSQRGRDGQHNFIVSLNPDVMPRLRVHDIYAGALRGHKGG
                     DGHQGMQQRLQEARWFIKNIQQRFDTILRVSRAIVERQKNFFTHGELAMRPLVLRDIA
                     DELGLHESTISRVTTAKYMATPIGTYELKYFFGSGLGTETGGNASSTAVRALIKQFVA
                     AESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL"
BASE COUNT          355 a          649 c          705 g          381 t
ORIGIN      
        1 gcacacggca ggaaaagttg gagttggttc atagacaggg caaaaggata cgcgcctacg
       61 gctgggcggc agaaacaggt gggcggctga gcagcgcgcg gatgtgtgcg ggcagcagcg
      121 ccaccttgcg atcggacttg ttgatgaaga cgatgccggt cttgccgcgt gccacttcgc
      181 ggccctgcga tttttcggtc atgcgaaaca ccaggtcaaa gccgtagggg ttgaaatcgg
      241 cgggagtcat ctccacacgc atggtctcgc catgaaagcc ctcggatttg tactgcgcca
      301 cgatgtcgcc caccacgatg gccagaccgc ccacatccgc ctcgggatag ccctgcgcct
      361 ggaaaaagcg cacgcgcgcc tcggacacca ggctgagcag ttgcgcattg tccagatgcc
      421 cgccctggtt cacatggctg atgtagatct gcatctcggt cgaaaagaca aagtgggcag
      481 gaagatcgaa aacgatgcgg gccatggcgt gagagacaaa taaaaaacag aacaaaggga
      541 tgacaaacgg cagctgacag acgcacgacc cacgcgctcg gcgggcaaaa acgtctacag
      601 cgccttgcgc aggttggccg gcgcgatctt gagcgcctcg cggtacttgg ccaccgtgcg
      661 gcgcgcgcac tcgatgccct gctccttgag catctcggcg atctggctgt cggacagcgg
      721 cttggcgggg ctttcggcgg ccacaaactg cttgatgagc gcgcgcacgg cggtactcga
      781 tgcattgcca cccgtttcgg tgcccaggcc cgagccaaag aaatacttca gttcgtaggt
      841 gccgatcggc gtggccatgt acttggccgt ggtcacgcgg ctgatggtgg actcgtgcag
      901 gcccagctcg tcggcaatgt cgcgcagcac cagcgggcgc atggccagct cgccatgggt
      961 gaagaagttc ttctgccgct cgacgatggc gcgcgagacg cgcaagatgg tgtcaaagcg
     1021 ctgctggatg ttcttgatga accagcgcgc ctcctgcagg cgctgctgca tgccctggtg
     1081 gccatcgccg cccttgtggc cgcgcagcgc gcccgcgtag atgtcgtgca cgcgcaggcg
     1141 cggcatcaca tcggggttga ggctgacgat gaagttgtgc tggccatcgc ggccccgctg
     1201 cgagccttcg cgcccggctt tgcgcacgat gacatcgggc acgatgatgt tgcgctccac
     1261 atcgacaaag cggcggccgg ggcgcggctc caggcgggca atcagggcca tggcggcgcg
     1321 ggtgcgttcc tcgccatcgc cacacagttg cgtgaggcgg cgaatgtcgc ggcgggccag
     1381 catctccagc ggctgctggc acacgcgcag cgcggtctgc acggtatcgg ggtcgattgc
     1441 gctgtcttcg tcggcagcca ttgccttgag ctgcagcgtg aggcactcgg ccaggtcgcg
     1501 tgcacccacg cccaccggct ccaggctctg cagcaggcgc tgggccacgg tgaaacggtg
     1561 caccagctcg tcgatctgct ccagatcgcc ctcgccggcc aggcctgtgg ccagctcttc
     1621 aagcggttct tcgaggtagc cgtcgtcgtt gagcgattcg atcagaaagc gcagcgcggc
     1681 gcggtcgatt tcggacaggc gcagcgccag ggcttggcgg tgcaggaagg acgtgagcga
     1741 ctcgtgcgag cgggccagct cggtggcatc ggcctcgtcg ccctcggagt cgttgcggtt
     1801 gcgggccggt gcgtcgccgc cccactcggc gtcgttgggt gccatttcca cggtgccatc
     1861 gccgctccag tcgggctcgt ccgcgctggc gctgggcgct tcagcatcat tttggccttc
     1921 agcgcttgtg ctgcttgcgc tagcagctcc tgaaaacaga gcatcgtcgg cactgtaatc
     1981 gtcggtctgc gcgggggtgt cggccgcttc aagaccaaac tcttcgcgtg gggcttcttc
     2041 ggccgtgcgc tcgagaaagg ggttttcgtc gagcatctgc tcgacttcct
//
LOCUS       DAOMVO010000397         2086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15735_length_2086_cov_13.804037, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000397 DAOMVO010000000
VERSION     DAOMVO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15735_length_2086_cov_13.804037"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PKV30_13270"
     CDS             <1..563
                     /locus_tag="PKV30_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL31222.1"
                     /translation="LLEMLRTGEIDCAILAEPFPDTGLAIAPLYDEPFMAALPAGHAL
                     AAGDSVSSEQLKREHMLLLGTGHCFRDHVLQVCPEFARFSSNTEGIRKSFEGSSLETI
                     KHMVAAGMGVTLVPRLAVPPEALAAAEPDAPGVVRYLPIHDDGGAPPTRRVVLAWRRS
                     FTRYEAIAALRNAIYACELPGVQRLS"
     gene            560..1390
                     /locus_tag="PKV30_13275"
     CDS             560..1390
                     /locus_tag="PKV30_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL31223.1"
                     /translation="MTAPVTTAMASAAAPLVPHEVANTVSQTQTALDPFAPWALLAVL
                     LLAAWALAAYRRRVLGPARPAAPRTDLARALTAVALLALPWAALVGALHASPPARWLT
                     QLDTQAAQWAQAVSTPALRRLAVFLSDIGDVLPLAVLTLIVAVWLWRRRQRLLAGAWL
                     LSITANALAIRVLKNLFARARPDTAAEMATSGHSFPSGHAAGALMVFGLLAWVLCDQL
                     DRRWHGLIVGAAALLIAGIAASRVLLGVHYFSDVLGGLLWAGMVLLLTLTIVRQAGRW
                     "
     gene            1447..>2086
                     /locus_tag="PKV30_13280"
     CDS             1447..>2086
                     /locus_tag="PKV30_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRL31224.1"
                     /translation="MTSVASPAAPRSKLSLYLDLIRFNRPAGWLLLLWPSLSALWLAA
                     DGFPGWHLVMVFTLGTILMRSAGCCINDVADREFDRHVKRTAQRPVTSGLISVKEALA
                     VGAVLALVAFGLVLTTNAATMAWSFPALAVTLIYPYAKRVVSMPQAVLGVAFSFGIPM
                     AYAAVQGRVPLEAWGLMLGNLFWVLAYDTEYAMVDRDDDLRIGMKTSAITLGR"
BASE COUNT          260 a          727 c          741 g          358 t
ORIGIN      
        1 gcctgctgga gatgctgcgc accggcgaga tcgactgcgc catcctggcc gagccttttc
       61 ccgacaccgg gctggccatt gcgccgctgt acgacgagcc cttcatggcc gcgctgccgg
      121 cggggcatgc tctggcggcg ggcgattcgg tcagcagcga acaactcaag cgcgaacaca
      181 tgctgctgct gggcaccggc cactgctttc gcgaccatgt gctccaggtg tgccccgagt
      241 tcgcgcgctt ttccagcaac accgagggga ttcgcaagag cttcgagggc tcgtcgctgg
      301 agaccatcaa gcacatggtg gccgccggca tgggcgtgac gctggtgccg cgcctggcgg
      361 tgccgcccga ggcgctggcc gcggccgagc ccgatgcgcc gggcgtggtg cgctacctgc
      421 cgatccacga cgacggcggc gcgccgccca cgcgccgcgt ggtgctggcc tggcgccgca
      481 gcttcacgcg ctacgaggcg attgccgcgc tgcgcaacgc catctacgcc tgcgaattgc
      541 cgggcgttca gcggctgtcg tgacggcccc ggtcaccacc gcgatggcca gcgcagcggc
      601 accgctcgtg ccgcacgagg tggcgaatac cgtatcgcag acgcagaccg cgctcgaccc
      661 cttcgcaccg tgggccttgc tggcggtgct gctgctggcg gcgtgggcgc tggccgctta
      721 tcgccgccgg gtgctgggcc cagcacgacc ggcggcacca cgcaccgacc ttgcgcgcgc
      781 gctgaccgcc gtcgcgctgc tggcgctgcc gtgggcggcg ctggtgggcg cgctgcatgc
      841 gtcgccgccc gcccgctggt tgacgcaact cgacacccag gccgcgcagt gggcgcaggc
      901 ggtgagcaca cccgcgctgc gccgcctggc ggtgttcttg agcgacatcg gcgacgtgct
      961 gccgctggcc gtgctcacgc tgatcgtcgc cgtgtggctg tggcgccgcc gccagcgtct
     1021 gctggccggg gcttggctgt tgtcgatcac cgccaatgcg ttggcgatcc gcgtgctgaa
     1081 gaacctgttt gcgcgcgcgc ggcccgacac ggcggcggag atggcgacgt cgggccacag
     1141 ttttcccagt ggccacgcgg ccggggcgct gatggtgttc gggctgctgg cctgggtgct
     1201 gtgcgaccag ctggatcgcc gctggcacgg tctcatcgtc ggtgccgccg cgctgctcat
     1261 cgccggcatc gcggccagcc gcgtgctgct gggcgtgcac tacttcagcg acgtgctggg
     1321 cggcctgctg tgggcgggca tggtgctgct gttgacgctg accatcgttc gccaagccgg
     1381 gcgttggtga caaaataagc cgctggcgca cgtctgacaa gcgcgagcag ctattaaatt
     1441 gattgcatga catccgttgc atcccccgcc gcaccgcgca gcaagctgtc gctgtacctg
     1501 gatctgatcc gcttcaaccg cccggccggc tggctgctgc tgctgtggcc gtcactcagc
     1561 gcgctgtggc tggcggcgga cggctttccg ggctggcatc tggtgatggt gttcacgctg
     1621 ggcaccatcc tgatgcgctc ggccggctgc tgcatcaacg acgtggccga ccgcgagttc
     1681 gaccgccacg tcaagcgcac cgcgcagcgc ccggtgacca gcgggctcat cagcgtgaag
     1741 gaagcgctgg ccgtgggcgc cgtgctggcg ctcgtcgcct ttggcctggt gctgaccacc
     1801 aacgccgcca ccatggcctg gtcgtttccg gcgctggccg tcacgctgat ctatccctac
     1861 gccaagcgcg tggtctcgat gccgcaggcg gtgctgggcg tggccttcag cttcggcatt
     1921 ccgatggcct acgcggcggt gcagggccgc gtgccgctgg aggcgtgggg gctgatgctc
     1981 ggcaacctgt tctgggtgct ggcctacgac accgaatacg ccatggtcga ccgcgacgac
     2041 gacctgcgca tcggcatgaa gacctcggcc atcacgctgg gccgct
//
LOCUS       DAOMVO010000398         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_15973_length_2063_cov_19.012948, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000398 DAOMVO010000000
VERSION     DAOMVO010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15973_length_2063_cov_19.012948"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKV30_13285"
     CDS             complement(<1..413)
                     /locus_tag="PKV30_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL31225.1"
                     /translation="MSQKVAYVTGGMGGIGTAICQRLSKDGFKVIAGCGPTRDYQKWL
                     DEQKAAGYTFYASVGNVGDWQSTVDAFSKAKAEHGPIDVLVNNAGITRDRMFLKMSPE
                     DWQAVIDTNLNSMFNVTKQVVPDMVEKGWGRIVQI"
     gene            complement(548..1726)
                     /locus_tag="PKV30_13290"
     CDS             complement(548..1726)
                     /locus_tag="PKV30_13290"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRL31226.1"
                     /translation="MEDIVIVSAARTGVGKFGGSLAKVPATELGSIAIKAALERAKVG
                     LDQVGEVIMGQVLAAGVGQNPARQALMKAGIAKETPALTINAVCGSGLKAVMLAAQAV
                     ATGDSEIVVAGGQENMSASPHVLLGSRDGQRMGDWKMIDTMIVDGLWDVYNQYHMGIT
                     AENVAKQEGITRDMQDELALNSQKKAAAAQAAGKFKDEIVPVSIPQRKGDPVVFDTDE
                     YINTKTNAEALAGLRPAFDKAGSVTAGNASGINDGAAAVVVMSAKKAAALGLTPLARI
                     ASYATSGLDPATMGLGPVPASQKALKRAGWAVGDVDLFELNEAFAAQACAVNKLLGAD
                     PAKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRSGAKKGLAGLCIGGGMGVALAVER
                     "
     gene            complement(1866..>2063)
                     /locus_tag="PKV30_13295"
     CDS             complement(1866..>2063)
                     /locus_tag="PKV30_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRL31227.1"
                     /translation="DGKFPKSVDEWIAGATETPGSWWDDWAGWLKGHAGKQIAAPKAY
                     GNGKQYPAIEPAPGRYVKAKA"
BASE COUNT          291 a          686 c          657 g          429 t
ORIGIN      
        1 ctgatctgca cgatgcggcc ccagcctttt tcaaccatgt cgggcaccac ctgcttggtg
       61 acgttgaaca tgctgttcag gttggtgtcg atcaccgcct gccagtcttc gggcgacatc
      121 ttcaggaaca tgcggtcgcg cgtgatgccg gcgttgttga ccagcacgtc gatggggccg
      181 tgctcggcct tggccttgct gaacgcatcg acggtcgact gccagtcgcc cacgttgccg
      241 acgctggcgt agaaggtgta gccggcggct ttttgctcgt ccagccactt ctggtagtcg
      301 cgcgtggggc cgcaaccggc gatgaccttg aacccgtcct tggacaggcg ctgacaaatg
      361 gcggtgccga tgccgcccat gccgccggtg acgtacgcta ctttctgact catgtgatgt
      421 ctcctctttt cacttcaaaa acaatagctg tctgcgcttt gttgacgccg acaacagcca
      481 atttacttca aaaaacctgc ccaagcttct aggggtttac gcaaggggcc tgagcgcctt
      541 gcatcgttca acgttccacc gccagcgcca cgcccatgcc gccgccgatg cacagcccgg
      601 ccaggccttt cttggcgccg ctgcgctgca tctcgtgcag cagcgtgacc aggatgcggc
      661 agccagaagc gccgatgggg tggccgatgg cgatggcacc gccgttgacg ttgaccttgg
      721 ccggatcggc acccagcagc ttgttgacgg cgcaggcctg cgcggcgaag gcttcgttca
      781 gctcgaacag gtcgacgtcg cccacggccc agccggcgcg cttgagcgct ttctgcgatg
      841 ccggcacggg gcccaggccc atggtggcgg gatcgagccc gctggtggcg tagctggcaa
      901 tgcgcgccag cggcgtgagg cccagcgcgg cggccttctt ggccgacatg acgaccacgg
      961 cggcggcgcc atcgttgatg cccgaggcgt tgcccgccgt cacgctgccc gccttgtcga
     1021 aggcggggcg caggccggcc agggcttcgg cgttggtctt ggtgttgatg tactcgtcgg
     1081 tgtcgaacac caccgggtcg cccttgcgct gcgggatgct gaccgggacg atctcgtcct
     1141 tgaacttgcc ggcggcctgc gcagcagcgg ccttcttctg gctgttcagc gccagctcgt
     1201 cctgcatgtc gcgcgtgatg ccttcctgct tggccacgtt ctcggcggtg atgcccatgt
     1261 ggtactggtt gtagacgtcc cacaggccgt ccacgatcat ggtgtcgatc atcttccagt
     1321 cgcccatgcg ctggccgtcg cggctgccca gcagcacgtg gggcgatgcg ctcatgttct
     1381 cctggccgcc ggccaccacg atctcgctgt cgccggtggc gacggcctgc gcggccagca
     1441 tcactgcctt caggcccgag ccgcacacgg cgttgatggt gagcgccggc gtttccttgg
     1501 cgatgccggc cttcatcagc gcctggcgcg ccgggttctg gcccacgcca gcggccagca
     1561 cctggcccat gatgacttcg cccacttggt cgaggccgac cttggcgcgc tccagcgcgg
     1621 ccttgatggc gatgctgccc agctcggtgg cgggcacctt ggccagcgag ccgccgaact
     1681 tgccgacgcc cgtgcgtgcg gcggaaacga tgacgatgtc ttccatctgc ttactctcct
     1741 gtttgctatc aaataacgag ctgcttgcgc ttgccgtgag ccgatttcag ttcgttttat
     1801 cttgaaatcg ttgcaggaca agcgcaagcc actatcaatt cagaagcgga tgcgatccgg
     1861 cttcttcacg ccttggcctt gacgtagcgg cccggcgccg gctcgatggc cgggtactgc
     1921 ttgccgttgc cataggcctt gggtgcggca atctgcttgc cggcgtggcc tttcagccag
     1981 ccggcccagt cgtcccacca gctgccgggt gtttcggtgg cgccggcgat ccactcgtcg
     2041 acgctcttgg ggaacttgcc gtc
//
LOCUS       DAOMVO010000399         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16140_length_2046_cov_13.265193, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000399 DAOMVO010000000
VERSION     DAOMVO010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16140_length_2046_cov_13.265193"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="PKV30_13300"
     CDS             <1..101
                     /locus_tag="PKV30_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HRL31228.1"
                     /translation="GQLISPDEAARRVLAYLARPDFGAEPVADVRG"
     gene            complement(193..1098)
                     /gene="ppk2"
                     /locus_tag="PKV30_13305"
     CDS             complement(193..1098)
                     /gene="ppk2"
                     /locus_tag="PKV30_13305"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRL31229.1"
                     /translation="MSEEELINRLRSDVLDGYDEELEMELEDRNIDQLAGVLTDASEY
                     KAARQQYFRELFRLQGELVKLQDWVANTKRKVVIIFEGRDAAGKGGVIKRITQRLNPR
                     VARVVALPAPNDREKTQWYFQRYVPHLPAGGEMVLFDRSWYNRAGVERVMGFCSDEEL
                     EEFFRTVPEFEKMLMRSGIVLIKYWFSISDDEQNLRFLGRIHDPLKQWKLSPMDLESR
                     RRWEDYTKAKEEMLERTHIPEAPWWIVPADDKKKARLNCITHLLSQLPYEEVERPEVV
                     LPPRMRNQDYVRHPVPQGMIVPQAY"
     gene            1260..>2046
                     /locus_tag="PKV30_13310"
     CDS             1260..>2046
                     /locus_tag="PKV30_13310"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRL31230.1"
                     /translation="MAREVVVAGGVRTAIGTFGGSLKDIAPVDMAATVVKEALARAKV
                     EGKDVAQVVFGHVVNTEPRDMYLSRVAAVNGGCPKETQAFNVNRLCGSGLQAIISASQ
                     GILLGDYDVAIGGGCENMSRAPYANLTNRFGARMGDTKLVDMMVGALHDPFHTIHMGV
                     TAENVAKEFEITREQQDALALESHQRAEKAWAEGRFKDQIVPIMLKSKKGDVAFEKDE
                     HFRAGAKLEDFQKMKPVFIKENGTVTAANASGINDAAAAVVLME"
BASE COUNT          356 a          680 c          640 g          370 t
ORIGIN      
        1 gcggccagct catctcgccc gacgaggccg cgcgccgcgt gctggcctat ctggcgcgcc
       61 cggacttcgg cgccgagccg gtggccgatg tgcgcggctg acgcgcggcc atcggtctca
      121 ctacagtttg aatagcggct tgcgcacgac ccgcaagcgc tggcggcctt tttggcttaa
      181 aaaacccgcc gctcaatacg cctgcggcac gatcatcccc tgcggcaccg ggtgccgcac
      241 gtagtcctgg ttgcgcatgc gcggcggcag caccacctcg ggccgctcga cttcctcgta
      301 cggcagttgg gacagcaggt gggtgatgca gttcaggcgc gcctttttct tgtcgtcggc
      361 cggcacgatc caccacggcg cttcggggat gtgggtgcgc tccagcatct cttccttggc
      421 cttggtgtag tcctcccagc ggcggcggct ttccaggtcc atggggctca gcttccactg
      481 cttgagcggg tcgtggatgc ggcccagaaa gcgcaggttc tgctcgtcgt cgctgatgct
      541 gaaccaatac ttaatgagca cgatgcccga gcgcatcagc atcttctcga actcgggcac
      601 ggtgcggaag aactcctcga gctcctcgtc cgagcaaaag cccatcaccc gctcgacgcc
      661 ggcgcggttg taccagctgc ggtcgaacag caccatttcg ccaccggccg gcaggtgcgg
      721 cacgtagcgc tgaaagtacc actgcgtttt ttcacggtcg ttgggcgccg gcagcgccac
      781 cacgcgcgcc acgcgcgggt tcaggcgctg ggtgatgcgc ttgatgacac cgcccttgcc
      841 ggccgcgtcg cgcccttcaa agatgatgac caccttgcgt ttggtgttgg ccacccagtc
      901 ctgcagcttg accagctcgc cctgcaggcg aaacagctcg cggaaatact gctggcgcgc
      961 cgccttgtat tcgctggcgt cggtcagcac cccggccagc tggtcgatgt tgcggtcttc
     1021 cagctccatc tccagctctt cgtcgtagcc gtccagcacg tcgctgcgca ggcggttgat
     1081 cagttcttct tcgctcactt gggctcgctc cagtgcatcg caggccgcca ccttaaccat
     1141 ggcgcgtgac agtctgatga ctgtcatatt cgcgccggcg ctgtcagggt tctgttgcct
     1201 gttggcggca tactgtgcgt tttatccagc ccgttcaacc acaagaggag actcgaatca
     1261 tggcccgtga agttgttgtt gcaggcggtg tgcgcaccgc catcggtact tttggcggca
     1321 gcctgaagga cattgccccg gtcgacatgg ccgccaccgt cgtcaaggaa gcgctggcgc
     1381 gcgccaaggt cgagggcaag gacgtggccc aggtcgtgtt cggccacgtg gtcaacaccg
     1441 agccgcgcga catgtacctg agccgcgtgg cggccgtcaa cggcggctgc ccgaaggaga
     1501 cgcaagcctt caacgtcaac cgcctgtgcg gctcgggcct gcaggccatc atctcggcca
     1561 gccaaggcat tctgctgggc gactacgacg tggccatcgg cggcggctgc gagaacatga
     1621 gccgcgcgcc ctatgccaac ctgaccaacc gctttggtgc gcgcatgggc gacaccaaac
     1681 tggtcgacat gatggtcggc gcgctgcacg accccttcca caccatccac atgggcgtga
     1741 cggccgagaa cgtggccaaa gagtttgaaa tcacccgcga gcagcaagac gcgctggcgc
     1801 tggagagcca ccagcgcgcc gaaaaagcct gggccgaagg ccgcttcaag gaccagatcg
     1861 tgcccatcat gctgaaaagc aagaagggcg acgtggcgtt cgagaaagac gagcacttcc
     1921 gcgccggcgc caagctggaa gacttccaga aaatgaagcc cgtcttcatc aaggaaaacg
     1981 gcaccgtgac cgccgccaac gccagcggca tcaacgacgc cgccgccgcc gtggtgctga
     2041 tggaag
//
LOCUS       DAOMVO010000400         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16337_length_2026_cov_21.488077, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000400 DAOMVO010000000
VERSION     DAOMVO010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16337_length_2026_cov_21.488077"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="PKV30_13315"
     CDS             complement(<1..1712)
                     /locus_tag="PKV30_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRL31231.1"
                     /translation="MTSVHTPPPPPTPLPAPAGWARLAPGLHVLRHYRREWLPRDVVA
                     GLVLSAILVPVGMGYAEAAGVPAIHGLYATIVPLLVYALLGPSRIMVLGPDSTLAAVI
                     ASLILPLAGGSVERAVALAGVLALMTGGFLLLIGFARLGVMADLFSKPIRLGFFNAIA
                     LTVMISQLPKLMGFSTTVNGSLPRLWEMVRGFVDGRGNPAAMAIGVACLALILGVRRW
                     RPKWPGVLIAVVLATTVSALFDLGATAGLDVIGPVPHGLPVPTLPLATVTLEDLRVLA
                     PGAAIIALLAFADTSVLSRALAQRSGTRVNPDQEMLALGSANLATGLMQGFPISSSNS
                     RTPVAASAGAQTQLANLVGAGVIALLLLLAPGLVKDMPQAALGAVVITACISFTDWPG
                     MFALLRQRQVEFMLAAASFLGVVFYGVIEGIAGAIGLSLLVMVWNAWHPYHTVLVRVD
                     GMKGYHDAARHPEGRLVPGLVLFRWDEQLFFANADLFREAVLQAVADAPTPTRQVIVA
                     ADAVNDIDVTAADMLGELDHELEARGIDLQFAGLKGHVRDLIIRYGLSPRFDAEHFHP
                     TVGNA"
     gene            complement(1709..>2026)
                     /locus_tag="PKV30_13320"
     CDS             complement(1709..>2026)
                     /locus_tag="PKV30_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL31232.1"
                     /translation="QIVFGLGWSGLMTAGEWALTDARIQWGWPLAAALLFVAVGPAVL
                     AYRCWGLGVQQAGPTVAGFFGNLTPVFAAVMSALFLGEAPRLYHALAFVLIVAGIVVS
                     ARR"
BASE COUNT          358 a          755 c          681 g          232 t
ORIGIN      
        1 gcgttgccca ccgtgggatg aaagtgctcg gcgtcgaagc gcgggctgag gccgtaacgg
       61 atgatgaggt cgcgcacgtg gcccttcagg cccgcgaact gcaggtcgat gccgcgcgct
      121 tcgagctcgt ggtccagctc gccgagcatg tcggcggcag tgacgtcgat gtcgttcacc
      181 gcgtcggccg ccacgatgac ctgccgcgtc ggcgtggggg cgtcggccac ggcctgcagc
      241 accgcttcgc ggaacaggtc ggcgttggca aagaacagtt gttcgtccca gcggaacagc
      301 accaagcccg gcaccaagcg gccctcgggg tggcgcgcgg cgtcgtggta gcccttcatg
      361 ccatcaaccc gcaccagcac ggtgtggtag gggtgccagg cgttccacac catgaccagc
      421 agcgacagcc cgatcgcgcc ggcaatgccc tcgatgacgc cgtagaacac cacgccgaga
      481 aagcttgccg ccgccagcat gaactcgacc tgccgctgac gcagcagcgc gaacatgcct
      541 ggccagtcgg tgaacgagat gcaggcggtg atgaccaccg cgcccagcgc cgcctgcggc
      601 atgtccttca ccagccccgg cgccagcaac agcagcagcg cgatcacgcc cgcgccgacc
      661 aggttggcca gttgcgtttg tgcgccggcg ctggcggcca ccggcgtgcg cgagttgctg
      721 ctgctgatcg gaaagccctg catcagcccc gtggccaggt tggcgctgcc cagcgccagc
      781 atctcctggt cggggttcac gcgcgtgccg ctgcgctgcg ccagcgcgcg cgacagcacg
      841 ctggtgtcgg caaacgccag cagcgcgatg atggccgcgc ccggcgccag cacgcgcaga
      901 tcctccagcg tgacggtggc cagcggcagc gtgggcaccg gcaggccgtg cggtaccggg
      961 ccgatcacgt ccagccccgc ggtcgcccca aggtcgaaca gcgccgacac cgtggtggcc
     1021 agcaccaccg cgatgagcac gccgggccac ttcggtcgcc agcgccgcac gcccaggatc
     1081 agcgccaggc aagccacccc gatggccatg gccgccgggt tgccgcggcc gtcgacgaag
     1141 ccgcgcacca tctcccacag gcgcggcagc gaaccattca ccgtggtcga aaagcccatc
     1201 agcttgggca actggctgat catcaccgtc agcgcgatgg cgttgaaaaa gccgagccgg
     1261 atcggcttgg aaaacaaatc ggccatcacg cccagccgcg caaagccgat cagcagcaag
     1321 aagccgccgg tcatcagcgc cagcacgccg gccagggcga cggcgcgctc cacgctgccg
     1381 ccggccagcg gcaggatcag gctggcgatg accgccgcca acgtcgaatc tggccccagc
     1441 accatgatgc ggctggggcc cagcagcgca tacaccagca gcggcacgat ggtcgcgtac
     1501 aagccgtgga tcgccggcac gccggccgcc tcggcataac ccatgcccac cggcaccaga
     1561 atggccgaca gaaccaggcc ggccaccacg tcgcgcggca gccattcgcg gcggtagtgg
     1621 cgcagcacgt gtagccccgg cgccaggcgc gcccagccgg cgggcgcggg cagcggcgtg
     1681 ggcggtggcg gcggcgtatg cactgacgtc atcggcgcgc cgacaccaca atgcccgcca
     1741 cgatcagcac gaacgccagc gcgtggtaca gccgtggcgc ctcgcccaga aaaagcgccg
     1801 acatgacggc ggcgaacacc ggcgtcaggt tgccgaagaa gcccgccacc gtcggccccg
     1861 cctgctgcac gcccaggccc cagcagcggt atgccagcac cgccgggccg acggccacga
     1921 acagcagcgc cgccgccagc ggccagcccc actggatgcg ggcgtcggtc agcgcccact
     1981 cgcccgccgt catcaggccc gaccagccga ggccgaacac gatctg
//
LOCUS       DAOMVO010000401         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16376_length_2023_cov_15.959350, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000401 DAOMVO010000000
VERSION     DAOMVO010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16376_length_2023_cov_15.959350"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKV30_13325"
     CDS             complement(<1..188)
                     /locus_tag="PKV30_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRL31233.1"
                     /translation="MTASLNLAQQDAVNHLRGPCLVLAGAGSGKTRVITHKIARLIQT
                     GVEGQRIAAITFTNKAAA"
     gene            427..1035
                     /locus_tag="PKV30_13330"
     CDS             427..1035
                     /locus_tag="PKV30_13330"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HRL31234.1"
                     /translation="MIKQWMKCAALSVALAVASPLALAQGSKAFAPTTQLAGQTLQLN
                     GQGVRYRAIIPVYEMALYTPKKVTTPEQLLGLPGPKRVSFVALRDVPGDQLGIALVRG
                     MSENADPGRAALIAGFMDKLGRIFSTEKKIAAGQAFALEHVPGKGTVFLINGVQKGET
                     VTDAGFIEAVLRIWVGPKPVDSQLKSSLLGAPPKKVDTNNLG"
     gene            1143..>2023
                     /locus_tag="PKV30_13335"
     CDS             1143..>2023
                     /locus_tag="PKV30_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL31235.1"
                     /translation="MTGILAVTFPFFALVLCGYVAARTRLLPLDAVPGLNMFVLYFAL
                     PCMLYRFGSATPAAQLFNPVIALLWLLAAALIVAATAGWARRQGGSWPDAAMGALVAA
                     FPNSGFMGVPLILALLGAGAVGPVMATLLVDLVVTTSLCIGLSQWGAAGEHGPAKAVG
                     RALQGVLRNPMPWSILLGALAGALGFALWAPLGKTVQLLADAASPVALFTIGAVLARS
                     QMRPATTAGQAAPRDVGVLAVTKLLLHPLLVWTMGTLAVRAGALDTSALTPLVLVAAL
                     PAASNVSLLAERFGADNG"
BASE COUNT          290 a          656 c          731 g          346 t
ORIGIN      
        1 tcggcggcgg ccttgttggt gaaggtgatg gcggcaatgc gctggccctc aacgccggtc
       61 tggatcaggc gcgcgatttt gtgcgtgatg acgcgcgtct tgcccgagcc cgcgccggcc
      121 agtaccaggc aaggtccgcg caagtgattg accgcgtctt gttgcgccag gttcagggat
      181 gcggtcatga aaaagccggc cagatcgcgg cggcgcgggg gagcagtgaa acggaggaag
      241 ctggcgatga tacccgccga cctgcggccg acaccctgtt cgggtgtgac ggaaatgttg
      301 caaacgcagg ggctaacgtg cggttgtcac tgttctgaca cctgcggcgc accccaacgg
      361 ggcgtcatgt cctctaatgg atacacgccg tccgcaggtg cgcggcgacc gaccggggag
      421 caagacatga tcaagcaatg gatgaagtgc gcggccttga gcgtcgcgtt ggccgtggcc
      481 agcccgctgg ccctcgcgca gggttcgaaa gcctttgcgc cgaccacgca actcgcaggc
      541 cagaccctgc aactcaacgg ccagggcgtg cgctaccgcg cgatcatccc ggtgtatgaa
      601 atggcgctgt atacgccgaa gaaggtgacc acgcctgaac aactgctcgg cctgccgggc
      661 cccaagcgcg tcagcttcgt cgcgctgcgc gatgtgccgg gcgaccagct gggcattgcg
      721 ctggtgcgcg gcatgagcga aaacgccgac ccagggcgcg cggcgctgat tgccggcttc
      781 atggacaagc tcggccgcat cttcagcacc gagaagaaga tcgccgccgg ccaggccttc
      841 gcactggagc atgtacccgg caagggcacc gtgttcctga tcaacggcgt gcaaaagggc
      901 gagaccgtca ccgatgccgg cttcatcgaa gccgtgctgc gcatctgggt cggccccaag
      961 ccggtcgaca gccagctgaa gagttcgctg ctgggcgcgc cgcccaagaa ggtcgacacc
     1021 aataacctgg gctgagcggc gctccgcacg cagcgcgctc cgggcgcgcc aagccggagg
     1081 ctggcccgcc agggcggcgc tgcagcgggc aattttgtta ccctcgccgc tccatgcaag
     1141 ccctgaccgg cattcttgcc gtcacctttc cgtttttcgc cctggtgctg tgcggctatg
     1201 tggctgcgcg cacgcggctg ctgccgctcg acgccgtgcc ggggctgaac atgttcgtgc
     1261 tgtactttgc gctgccgtgc atgctgtacc ggtttggcag cgcgacgccg gcggcgcagc
     1321 tgttcaatcc cgtcatcgcg ctgctgtggc tgctggcggc ggcgctcatc gtggccgcca
     1381 ccgccggctg ggcgcgccga cagggcggct cgtggcctga tgccgccatg ggcgcgctgg
     1441 tggcggcttt cccaaattcg ggcttcatgg gcgtgccgct gatcctggcg ctgttgggcg
     1501 cgggtgccgt cggcccggtg atggcgacgc tgctggtcga cctggtggtg acgacgtcgc
     1561 tgtgcatcgg cctgtcgcaa tggggcgcgg caggcgaaca cgggccggcc aaggcggtcg
     1621 gccgggcgct gcaaggcgtg ctgcgcaacc ccatgccgtg gtcgattctg ctgggcgcgc
     1681 tggccggtgc gctgggtttc gcgctgtggg caccgctcgg caagacggtg cagttgctgg
     1741 ccgatgcagc atcgcccgtg gcactgttca ccatcggcgc cgtgctggcg cgctcgcaga
     1801 tgcggccggc gaccacggcc ggccaggcgg cgccgcgcga tgtcggcgtg ctggccgtta
     1861 ccaagctgtt gctgcacccg ctgctggtgt ggacgatggg cacgctggcg gtgcgcgcgg
     1921 gggctctgga cacgtctgcc ctgacaccgt tggtgctggt ggcggcgctg ccggcggcca
     1981 gcaatgtgtc gctgctggcc gagcgctttg gcgccgacaa cgg
//
LOCUS       DAOMVO010000402         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16419_length_2020_cov_17.274300, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000402 DAOMVO010000000
VERSION     DAOMVO010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16419_length_2020_cov_17.274300"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKV30_13340"
     CDS             <1..266
                     /locus_tag="PKV30_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRL31236.1"
                     /translation="LPPQGQVVTAPLPGRPLAAPVIAPARIGLREAQSLLAGMGYDPG
                     PADGVAGRRTTAALRAFQRDHGLPTSGRLDDATRQALARPSPR"
     gene            complement(326..1753)
                     /locus_tag="PKV30_13345"
     CDS             complement(326..1753)
                     /locus_tag="PKV30_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRL31237.1"
                     /translation="MNAPAQLAHVMPEVNQRPVPPALIDALKSRFGAQFSEALVVREQ
                     HGRDESAFTTVPPPAGVVFAESTADVADAVKLCAAHKVPVIPYGVGSSLEGHLLAVQG
                     GISIDVSRMNKVLAVNAEDLTVTVQPGVTRKALNDEIKSTGLFFPIDPGADASIGGMT
                     ATRASGTNAVRYGTMRENVLALEVVTASGDVIRTGTRAKKSSAGYDLTRLFVGSEGTL
                     GVITEVTLRIYPLPEAVSAAICSFTSIGDAVNTVIQTIQLGVPIARVELIDANSVRAV
                     NQHSKLTLREQPLLLMEFHGSPASVKEQAEVVQDIASEHGGADFEWADTPEERTRLWT
                     ARHNAYFAAVQSRAGCRCITTDTCVPISRLADALLDTVAEADASGIPYFLVGHVGDGN
                     FHMGYLIDPDDADERERAEQLNHQVVARALALGGTCTGEHGVGLHKMGFLVDEAGVGA
                     VAMMRTIKQALDPDNIMNPGKVFSL"
BASE COUNT          294 a          704 c          670 g          352 t
ORIGIN      
        1 gcctgccgcc gcagggccag gtggtgacgg cgccgctgcc cggccggccg ctggccgcgc
       61 ccgtgatcgc ccccgcgcgc atcggcctgc gcgaggcgca gagcctgctg gccggcatgg
      121 gctacgaccc cggcccggcc gatggcgtgg ccggccgccg caccaccgcg gcgctgcgcg
      181 cctttcagcg cgaccatggc ctgccgacca gcggccgcct ggacgacgcc acgcgccagg
      241 cgctggcccg cccaagcccg cgctgattgc tattgaaaaa gaagctgtta gcgctcgctg
      301 gacaagcgcc cgcgccgttt ttcgcttaaa gcgagaacac cttgcccggg ttcatgatgt
      361 tgtccggatc cagcgcctgc ttgatggtgc gcatcatcgc caccgcgccg acgccggcct
      421 cgtcgaccag aaagcccatc ttgtgcagcc ccacgccgtg ctcgccggtg caggtgccgc
      481 ccagcgccag cgcgcgcgcc accacctggt gattgagctg ctcggcgcgc tcgcgctcgt
      541 cggcatcgtc ggggtcgatc aggtaaccca tgtggaagtt gccgtcgccc acatgcccga
      601 ccagaaagta cgggatgccc gatgcatcgg cctccgccac cgtgtccagc agcgcatcgg
      661 ccaggcggct gatcggcacg caggtatcgg tggtgatgca gcggcagccg gcgcggcttt
      721 gcacggcggc aaagtaggcg ttgtgccgcg ccgtccacag gcgcgtgcgc tcttcgggcg
      781 tgtcggccca ttcaaagtcg gcgccgccgt gttcgctggc aatgtcctgc accacctcgg
      841 cctgctcctt cacgctggcc ggtgagccgt gaaattccat cagcagcagc ggctgctcgc
      901 gcagcgtcag cttgctgtgc tggttgacgg cgcgcacgct gttggcgtcg atcagctcca
      961 cgcgcgcgat cggcacgccc agctgaatgg tctggatgac ggtgttgacc gcgtcgccga
     1021 tgctcgtaaa actgcagatc gccgccgaca ccgcctcggg cagcgggtag atgcgcagcg
     1081 tgacttcggt gatcacgccc agcgtgcctt cgctgccgac gaacaggcgc gtcaggtcgt
     1141 agccagcgct gctcttcttg gcccgcgtgc cggtgcggat cacatcaccg ctggcggtga
     1201 ccacttccag cgccagcacg ttctcgcgca tggtgccgta gcgcacggcg ttggtgccgg
     1261 atgcgcgcgt cgccgtcatg ccgccgatgc tggcgtcggc gcccgggtcg atgggaaaga
     1321 acaggcccgt gctcttgatc tcgtcgttga gtgccttgcg cgtgacgcct ggctgcaccg
     1381 tcaccgtcag gtcttcggcg ttcacggcca gcaccttgtt catgcggctg acgtcgatgc
     1441 tgatgccgcc ctgcaccgcc agcaaatgcc cttccagcga cgagccgacg ccgtagggaa
     1501 tcaccggcac cttgtgcgcg gcgcacagct tcaccgcatc ggccacgtcg gccgtgcttt
     1561 cggcaaacac cacaccggcc ggcggcggaa cggtggtgaa ggccgattca tcacgtccgt
     1621 gctgctcgcg caccaccagc gcttcggaga actgcgcgcc gaagcggctt ttgagtgcgt
     1681 cgatcagcgc gggggggacc gggcgctggt tgacttcggg catgacgtgg gcgagttggg
     1741 cgggggcgtt catggggtgt ctcctggcgg cggcggccgc tggcaatctc ggtcggccag
     1801 tttaagccga tcaggcgaca ggtgctcagg gatgtcatct tgggcatcac tttgattttg
     1861 atagctgttc gcgctggatg gacaagcgct taagcccgat ttgaacttga tctgcgaggt
     1921 cgtacggctg cgctaaagtg gcacctgcca gatcaacctc ccgccccatg cccgaccgct
     1981 acgccgtcat cggccacccc atcgcccaca gcaagtcgcc
//
LOCUS       DAOMVO010000403         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16544_length_2007_cov_12.978996, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000403 DAOMVO010000000
VERSION     DAOMVO010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16544_length_2007_cov_12.978996"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..581
                     /locus_tag="PKV30_13350"
     CDS             36..581
                     /locus_tag="PKV30_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL31238.1"
                     /translation="MPAARSLQRACHRRARAPARMTGITVRRADYTDARDRAALLDLL
                     DAYAQDPMGGGEPLPASTRARLCDDLAARPLAASFIAWLGDEPVGLVNTFEGYSTFKA
                     RPLLNVHDIAVLPAHRARGVGQALLAACEAHARERGCCKLTLEVLSGNARALRSYERF
                     GFAPYALDPAAGQALLLQKWL"
     gene            698..>2007
                     /locus_tag="PKV30_13355"
     CDS             698..>2007
                     /locus_tag="PKV30_13355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HRL31239.1"
                     /translation="MNAPDHPTWHPAAWRERPARQMPVYPDPARLDAVCNQLRQFPPL
                     IFDGEVRALRQQLAEVAAGRAFLLQGGDCAESFDTLDGESARETFRVLLQMAVVLTYA
                     AARPVVKVGRIAGQYAKPRSADSETQGGVTLPSYRGDIVNGAGFTADERTPQPERLLR
                     AYSASAATLNLLRALAQGGFADLHQLHAWTTDFVRASPQGQRYAELAGRIGETLAFMA
                     AMGFTSDNTPQLREVSFYTSHEALLLPYEEALTRPCEGAGGGWFGASAHMLWLGERTR
                     QLDGAHIDYLRGIANPVGVKIGPTATPDEVLALLDALDPAHEPGRVVLITRMGAGKLP
                     TLLPPLVRAVRDAGRPVIWSCDPMHGNTVTSANGYKTRDFANVVQEVREFFAVHAAEG
                     TVAGGLHVELTGQDVTECRGGGQNLGDEQLQQRYTTACDPRLNGS"
BASE COUNT          312 a          732 c          666 g          297 t
ORIGIN      
        1 gacaacgtct acggtgaccg caacctgttc tgcagttgcc tgccgctcga agccttcagc
       61 gagcctgcca tcgccgcgcc cgagcccctg cccgcatgac cggcatcacg gttcggcgcg
      121 ccgactacac cgacgcgcgc gaccgcgcgg cgctgctcga cctgctggat gcctacgcgc
      181 aagacccgat gggcggcggc gagccgctgc ctgcgtccac gcgcgcgcgc ctgtgcgacg
      241 acctggcagc gcgcccgctg gcggccagct tcatcgcctg gctgggcgat gagccggtgg
      301 gcctggtcaa caccttcgag ggctactcca ccttcaaggc gcgcccgctg ctcaacgtgc
      361 acgacatcgc cgtgctgccg gcgcaccgcg cacgcggcgt cggccaggcg ctgctggccg
      421 cgtgcgaggc gcatgcgcgc gaacgcggct gctgcaagct gacgctggaa gtgctgagcg
      481 gcaacgcgcg cgccctgcgc agctacgagc gctttggctt cgcgccctac gcgctcgacc
      541 ccgccgccgg ccaggcgctg ttgctgcaaa aatggttgta ggcgtgcccg gcattcaggg
      601 cagctccaag ccatttctat caaaaacata gctgcccgcg ctttccagac aagcgctgga
      661 gcctagaatc ttcgaaaatt ctctggcgaa acaccccatg aacgcccccg atcaccccac
      721 ttggcacccc gccgcctggc gcgagcggcc ggcgcgccag atgcccgtgt accccgaccc
      781 ggcgcggctc gatgccgtgt gcaatcaatt gcggcagttt ccgccgctca tcttcgacgg
      841 cgaggtgcgc gcgctgcgcc aacaactggc cgaggtcgct gccggccgag ccttcttgct
      901 gcaaggcggc gattgcgcgg agagcttcga cacgctcgat ggcgaatcgg cgcgcgagac
      961 ttttcgcgtg ctgctgcaga tggcggtggt gctgacctac gccgctgcgc gacccgtggt
     1021 caaggtgggc cgcatcgccg ggcagtacgc caagccgcgc tcggccgata gcgagactca
     1081 gggcggcgtg accctgccga gctaccgggg cgacatcgtc aacggcgcgg gctttacggc
     1141 cgacgaacga actccgcagc ctgagcgcct gctgcgcgcc tactcggcca gcgctgccac
     1201 gctcaacttg ctgcgcgcgc tggcccaagg cggctttgcc gatctgcacc agctacacgc
     1261 gtggaccacc gacttcgtgc gcgccagccc gcaaggccag cgctatgccg agctggcggg
     1321 ccgcatcggc gagacgctgg ccttcatggc ggcgatgggc ttcaccagcg acaacacgcc
     1381 gcagttgcgc gaggtgagct tctacacctc gcacgaagcc ttgctgctgc cgtatgagga
     1441 agcgctgacg cggccttgcg aaggcgctgg cggcggctgg tttggcgcct cggcccacat
     1501 gctgtggctg ggcgagcgca cgcgccagct ggatggcgcg cacatcgact acctgcgcgg
     1561 catcgccaac ccggtgggcg tcaagattgg ccccacggcc acgcccgatg aggtgttggc
     1621 gctgctcgat gcgctcgacc ccgcgcacga acccggtcgc gtggtgctca tcacccgcat
     1681 gggcgccggc aagctgccca cgctgctgcc gccgctggtg cgcgcggtgc gcgatgcagg
     1741 ccgccccgtg atctggagct gcgaccccat gcacggcaac accgtgacca gcgccaacgg
     1801 ctacaagacg cgcgacttcg ccaacgtggt gcaggaagtg cgcgagttct tcgccgtgca
     1861 tgccgccgaa ggcaccgtgg ccggcggcct gcatgtggag ctgaccggcc aggacgtgac
     1921 cgaatgccgc ggcggcggcc agaacctggg cgacgaacaa ctgcaacagc gctacaccac
     1981 cgcctgcgat ccgcgcctga acggctc
//
LOCUS       DAOMVO010000404         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16795_length_1983_cov_16.334025, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000404 DAOMVO010000000
VERSION     DAOMVO010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16795_length_1983_cov_16.334025"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKV30_13360"
     CDS             complement(<1..666)
                     /locus_tag="PKV30_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRL31240.1"
                     /translation="MTEELFRQDATLTECAATVLDVTEQGIVLGRTVFYPLGGGQAGD
                     SGWLVLADGAELVIADTRKEKDEAGVATGRICHLPAPEQREQLSKIKQGDAVTARIDA
                     PRRQRLMRFHTASHLLCHLVPQPVNGCSITPDHARIDFHMTEPLDKEQLTAGLARLVA
                     AALPVGVGSVTDAELDTNPQLVKSMSVQPPRGSGTVRAIRIGSADLIDMQPCGGTHVA
                     NTAE"
     gene            704..1546
                     /locus_tag="PKV30_13365"
     CDS             704..1546
                     /locus_tag="PKV30_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRL31241.1"
                     /translation="MPQNLPKLPPRSRHWSDLITTDFATLDLARAIAVLPLGATEQHG
                     PHLPLSVDANLAHSMVQIAATHLPPDLPVLFLPVQRIGYSPEHTAFAGTLSLKADTVM
                     RLWTDIAESVSASGVNKLVLFNTHGGHVGLMDVVARELRTRLGMLVYSVSWFNLPLKS
                     PDGEDLMTRFSADEQRFGVHGGQIETAMMLALKPRLVRQSKAQDFASSSQQRARDFAL
                     LGNGKSAKLAWAAQDLNPDGAAGNASAANVDDGLALVDAAGRALAALLVEIDRLPADT
                     LRGR"
     gene            1625..>1983
                     /locus_tag="PKV30_13370"
     CDS             1625..>1983
                     /locus_tag="PKV30_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRL31242.1"
                     /translation="MTSRRRFTATLLASTVVAAAPSAWAANEPPIHVLVGFPAGGGSD
                     VIARLLAERLEKELGRTVLVENRPGAGGQIAAQLLKAAKPDGTTVFLSHDHTISILPQ
                     VVKKPGYAPHEDFTPLGG"
BASE COUNT          292 a          704 c          672 g          315 t
ORIGIN      
        1 ctctgccgtg ttggccacgt gcgtgccgcc acagggctgc atgtcgatca agtcggcgct
       61 gccaatgcgt atcgcacgca ccgtgccgct gccgcgcggc ggttgcacgc tcatgctttt
      121 caccagttgc ggattggtgt ccagctcggc atcggtcacg ctgcccaccc ccaccggcag
      181 cgcggcagcg accaggcgcg ccaggccggc ggtgagctgt tccttgtcga gcggctcggt
      241 catgtggaag tcgatgcgcg cgtggtcggg cgtgatcgag cagccattga ccggctgcgg
      301 caccaggtgg cacagcaggt gcgaggcggt gtgaaagcgc atcagccgct ggcggcgcgg
      361 ggcgtcgatg cgggctgtga ccgcatcgcc ttgctttatt tttgagagct gctcgcgctg
      421 ctctggcgcg ggcaggtggc agattcggcc tgtggcgacg cccgcctcgt ctttttcctt
      481 gcgcgtgtcg gcgatcacca attcggcgcc atcggccagc accagccagc cgctgtcgcc
      541 ggcctggccg ccgcccagcg ggtagaacac ggtgcggccc agcacgatgc cctgctcggt
      601 cacatccagc acggtggcgg cgcattcggt cagcgtggcg tcctggcgaa acagctcttc
      661 ggtcatgcgc caaatgatag tggcggggcg taacatcgcg cccatgccgc agaacctgcc
      721 caagctgcca ccgcgcagcc gccactggtc ggacctgatc acgacggatt tcgccacgct
      781 cgacctggcg cgcgccattg ccgtgctgcc gctgggcgcg accgaacagc acgggccgca
      841 cttgccgctg tcggtcgatg ccaacctggc gcacagcatg gtgcagatcg cggccacgca
      901 cctgccgccc gatctgccgg tgctgtttct gccggtgcaa cgcatcggct acagccccga
      961 acacaccgcc tttgccggca cgctgagcct gaaggccgac accgtgatgc gcctgtggac
     1021 cgacatcgcc gaaagcgtgt cggccagcgg cgtgaacaag ctggtgctgt tcaacacgca
     1081 cggcggccac gtcgggctga tggacgtggt ggcgcgcgag ctgcgcacgc ggctgggcat
     1141 gctggtctac agcgtgagct ggttcaacct gccgctcaaa agccccgatg gcgaggattt
     1201 gatgacgcgc ttttcggccg acgagcagcg ctttggcgtg cacggcggcc agatcgaaac
     1261 ggccatgatg ctggcgctga aaccccggct ggtgcgccag tcgaaggcgc aggactttgc
     1321 ctccagctcg cagcagcgcg cgcgcgactt cgccctgctg ggcaacggca agagcgccaa
     1381 gctggcctgg gccgctcagg acttgaaccc cgacggcgcc gccggcaacg ccagcgccgc
     1441 caacgtcgac gatgggctgg cgctggtcga cgctgccggc cgcgcgctgg cggcgctgct
     1501 ggtcgagatc gaccggctgc cggccgatac gttgcgcggg cgctgagcgc gctgccccct
     1561 ggcccaccct tcattaaaat tgccggcttt gccccagccc ggggctgctt ctgggagagc
     1621 ctgcatgacc agccgccgcc gcttcaccgc caccctgctc gccagcaccg tcgtggccgc
     1681 cgcaccctcg gcctgggccg cgaacgaacc gccgatccac gtgctggtgg gctttccggc
     1741 cggcggcggc tcggacgtga tcgcgcgcct gctggccgag cggctggaaa aagagctggg
     1801 ccgcaccgtg ctggtggaaa accgccccgg cgccggcggc cagatcgccg cccagctgct
     1861 gaaggccgcc aagcccgacg gcaccaccgt ctttctgtcg cacgaccaca ccatctcgat
     1921 cctgccgcag gtggtcaaga agcccggcta cgcgccgcac gaggacttca cgccgctggg
     1981 cgg
//
LOCUS       DAOMVO010000405         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16870_length_1975_cov_18.037500, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000405 DAOMVO010000000
VERSION     DAOMVO010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16870_length_1975_cov_18.037500"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="rsmA"
                     /locus_tag="PKV30_13375"
     CDS             <1..819
                     /gene="rsmA"
                     /locus_tag="PKV30_13375"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRL31243.1"
                     /translation="GQHFLADPAIIDAIVRAIDPRPGQAMVEIGPGLAALTQPLVERL
                     GRLTVIELDRDLAARLRAHPQLDVIESDVLKVDFAQLQQALAATKFAAAQGSSKQGAA
                     PLGGSEPRAAGSVGALRVVGNLPYNISTPILFHLLPFAAHIEDQHFMLQKEVIDRMVA
                     PPATSDYGRLSVMLQWRYAMENVLFVPPESFDPPPRVDSAVVRMIPRADFVPVDPALL
                     SEVVQVAFSQRRKLLRHSLGKWLEARGFSGDFDVQRRAEEVPVPEYLALTQVLQ"
     gene            complement(868..1836)
                     /locus_tag="PKV30_13380"
     CDS             complement(868..1836)
                     /locus_tag="PKV30_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL31244.1"
                     /translation="MIDVTLQNFEADVLQASTRAPVLLDVWAEWCGPCKQLGPVLEQL
                     EAEYAGRFTLAKLDADKVPEIAGQLSQMLGTRSIPLCVLFAGGQPVDAFVGAQTPAQI
                     RQFLDKHVPTEGALAAEEEVSEAEALIAAGDTQAALTKLADALAADPGNDDARADYVR
                     LLIATGGYEEAAAALEEPLSREPRPLRFDALRRWLDAMEFVEKSERGNWPLPQFDEEI
                     AKNKRDFDTRYQKAQVLMAEGQWPQAMDELLEIIMRDRKWNDEAARKTYVAILELLTP
                     PPTKAGAGADGKSAGGIELTGKAAKQEDPQAELLSSYRRKLSMALN"
     gene            1893..>1975
                     /locus_tag="PKV30_13385"
     CDS             1893..>1975
                     /locus_tag="PKV30_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRL31245.1"
                     /translation="MTNPIVGVVMGSSSDWDTMQHAVQILE"
BASE COUNT          312 a          661 c          644 g          358 t
ORIGIN      
        1 ggccagcact tcctggccga cccggccatc atcgacgcca tcgtgcgcgc catcgacccg
       61 cgcccgggcc aggccatggt cgaaatcggc cccggcctgg cggcgctgac gcagccgctg
      121 gtcgagcggc tggggcgcct cacggtgatc gaactcgacc gcgacctggc cgcgcggctg
      181 cgtgcgcatc cgcagttgga tgtcatcgaa tccgatgtgc tgaaagtgga ctttgcgcag
      241 ctccagcaag cgctggcagc tacaaaattc gctgcagcgc agggcagctc caagcaaggc
      301 gcggccccct tggggggcag cgaaccacgc gcagcgggga gcgtgggggc tttgagagtc
      361 gtgggcaacc tgccctacaa catctccacc ccgatcctgt ttcacctgct gccctttgcc
      421 gcgcacattg aagaccagca cttcatgctg caaaaggaag tcatcgaccg catggtggcg
      481 ccgcccgcca ccagcgatta cggccgcctg tcggtcatgc tgcagtggcg ctacgcgatg
      541 gaaaatgtgc tcttcgtgcc acccgagtcc ttcgacccgc caccgcgcgt cgactcggcc
      601 gtggtgcgca tgatcccgcg cgccgacttc gtgccggtcg atcccgcgct gctgtccgaa
      661 gtcgtgcaag tcgccttcag ccagcgccgc aagctgctgc gccattcgct gggcaagtgg
      721 ctggaggcgc gcgggttttc tggtgacttc gatgtgcagc ggcgcgccga agaggtgccg
      781 gtgcccgaat atttggcact gactcaggtg cttcaataaa aagagcgact cgcgcttgtc
      841 tgacaagcgc tggcgcctga aaaaccttta gttcaaggcc atcgacagct tgcgccggta
      901 actcgacagc agctcggcct gcgggtcttc ctgcttggct gccttgccgg tcagctcgat
      961 gccgccagcg ctcttgccgt cggcgcctgc gccagccttg gtcggtggcg gggtcagcag
     1021 ctccaggatg gcgacgtagg tcttgcgcgc ggcctcgtcg ttccacttgc ggtcgcgcat
     1081 gatgatttcg agcagctcgt ccatcgcctg cggccactgc ccttcggcca taagcacctg
     1141 cgccttctgg tagcgggtgt cgaagtcgcg cttgttcttg gcaatctcct cgtcaaactg
     1201 cggcagcggc cagttgccac gctcgctttt ctcgacgaat tccatggcgt ccagccagcg
     1261 gcgcagggcg tcgaagcgca gcgggcgcgg ctcgcgcgac agcggctctt ccagcgcggc
     1321 cgcggcttct tcgtagccgc cggtggcgat caacaggcgc acgtagtcgg cgcgcgcgtc
     1381 gtcgttgccg gggtcggcgg ccagcgcgtc ggccagcttg gtgagggcgg cttgcgtgtc
     1441 gccggcggcg atcagcgcct cggcctcgct cacttcttct tcagccgcca gcgcgccttc
     1501 agtcggcaca tgcttgtcga ggaactggcg aatctgcgcc ggcgtttgcg cgccgacaaa
     1561 ggcatcgacc ggctgaccgc ccgcaaacag cacgcacagc gggatgctgc gcgtgcccag
     1621 catctgcgac agctggccgg cgatttcggg caccttgtcg gcatcgagct tggccagggt
     1681 gaagcggccg gcgtattcgg cctcgagctg ctccagcacc ggcccgagct gcttgcacgg
     1741 gccgcaccat tcggcccaca cgtcgagcag cacgggcgcg cgcgtggagg cctgaagcac
     1801 gtcggcttcg aagttttgca gggtgacgtc gatcatcgtg tgtatctgag ggcaaaatcg
     1861 ggcaaaccaa caaccattgt gcaagaagcc gcatgacgaa tcccatcgtg ggggtggtca
     1921 tgggttccag cagcgactgg gacaccatgc agcacgccgt ccagatcctt gagca
//
LOCUS       DAOMVO010000406         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16960_length_1969_cov_16.197492, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000406 DAOMVO010000000
VERSION     DAOMVO010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16960_length_1969_cov_16.197492"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /gene="tsf"
                     /locus_tag="PKV30_13390"
     CDS             <1..499
                     /gene="tsf"
                     /locus_tag="PKV30_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRL31246.1"
                     /translation="GLIGKIGENMSFRRFVRKAGKVASYVHGTRIGVLVEYTGDEAAA
                     KDVAMHVAAMKPVALASSDVPAELIEKERSVATAKAAESGKPADIATKMIEGAVQKYL
                     KEVSLFNQPFVKNDKQSVEQMLKEKGTTVTGFTLYVVGEGIEKKQDDFAAEVAAQVAA
                     AQGKA"
     gene            608..1330
                     /gene="pyrH"
                     /locus_tag="PKV30_13395"
     CDS             608..1330
                     /gene="pyrH"
                     /locus_tag="PKV30_13395"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRL31247.1"
                     /translation="MTKAKPAYKRILLKLSGEALMGDDAFGINRATIERIVAEIAEVT
                     QMGVQVAVVIGGGNIFRGVAGGSAGMDRATADYMGMLATVMNALALADAMNHQGLVAR
                     VMSAIAIEQVVEPYVRPKALQYLEEGKVVVFAAGTGNPFFTTDTAAALRGAEIGAELV
                     LKATKVDGVYTADPHKDKTATRYAKLSFDEAMTRNLGIMDATAFALCRDQKLPIKVFS
                     IFKPGALKAVVQGADEGTLVHA"
     gene            1413..>1969
                     /gene="frr"
                     /locus_tag="PKV30_13400"
     CDS             1413..>1969
                     /gene="frr"
                     /locus_tag="PKV30_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRL31248.1"
                     /translation="MTIADIKQTTQGKMDQSIASFKHNLTKIRTGRASPALLDTIHVE
                     YYGNHVPLSQVANVSLLDARTISVQPWEKGMGAKIEKAIRESDLGLNPASMGDLIRVP
                     MPPMSEERRKEMTKIVRSEGENAKIAIRNLRRDANDSIKRLVKDKEATEDDQKRSETE
                     IQKATDKHIAEIDALVVSKEKDIMEV"
BASE COUNT          428 a          626 c          636 g          279 t
ORIGIN      
        1 cggcctgatc ggcaagatcg gcgagaacat gagcttccgc cgcttcgtgc gcaaggccgg
       61 taaggtcgcc agctatgtgc atggcacgcg catcggcgtg ctggtggagt acaccggcga
      121 cgaagccgcc gccaaggacg tggccatgca cgtggccgcc atgaagccgg tggcgctggc
      181 gagcagcgac gtgccggccg agctgatcga gaaagagcgc agcgtggcca ccgccaaggc
      241 ggccgaatcc ggcaagccgg ccgacatcgc caccaagatg atcgaaggtg cggtgcagaa
      301 atacctgaag gaagtctcgc tgttcaacca gcctttcgtg aagaacgaca agcaatcggt
      361 cgagcagatg ctgaaggaga aaggcaccac ggtgaccggc ttcaccctgt acgtggtggg
      421 cgagggcatc gagaagaagc aggacgactt cgccgccgaa gtggccgcgc aagtcgctgc
      481 tgcgcaaggc aaggcctgac agtggccgat ggtgcccacg ccttgagtgc gcgtgggcgc
      541 catggcccca gaaggacgtc ccaccacgcc taaactcccg cccgtcgcca cctctggagc
      601 ttcgcccatg accaaagcca aacccgccta caagcgcatc ttgctcaagc tctctggcga
      661 ggcgctgatg ggcgacgacg cattcggcat caaccgcgcg accattgaac gcatagtggc
      721 cgagatcgcc gaagtcactc agatgggcgt gcaggtggcg gtggtgattg gcggcggcaa
      781 catctttcgc ggcgtggcgg gcggatcggc cggcatggac cgcgccaccg ccgactacat
      841 gggcatgctg gccacggtga tgaatgcgct cgcgctggcc gacgccatga accaccaggg
      901 cctggtggcg cgcgtgatga gcgccatcgc catcgagcag gtggtcgagc cctacgtgcg
      961 ccccaaggcg ctgcagtatc tggaagaagg caaggtggtg gtgtttgccg ccggcaccgg
     1021 caaccccttc ttcaccaccg acaccgccgc tgcgctgcgc ggcgccgaga tcggggccga
     1081 actggtgctg aaagcgacca aggtcgatgg cgtctacacc gccgatccgc acaaggacaa
     1141 gacggccacg cgctacgcca agctcagttt cgacgaggcc atgacgcgca acctgggcat
     1201 catggacgcc accgccttcg cgttgtgtcg cgaccagaag ctgccgatca aggtgttctc
     1261 gatcttcaag cccggggcgc tgaaggcggt ggttcagggc gctgacgaag gcactttggt
     1321 gcacgcctga tttcgtgcgc accgcctgag cggccgccca ttcgcgcgcg gcctgacgcg
     1381 ccgtgataat ccgttgttgg agcaaaccag acatgacgat tgccgacatc aagcaaacca
     1441 cgcaaggcaa gatggaccag tccatcgcct ctttcaagca caacctgacc aagatccgca
     1501 ccggccgcgc cagcccggcg ctgctggata ccattcacgt cgagtactac ggcaaccatg
     1561 tgccgctgtc gcaggtggcc aacgtgtcgc tgctcgacgc gcgcaccatc agcgtgcagc
     1621 cgtgggaaaa aggcatgggc gcgaagatcg aaaaggccat ccgcgaaagc gacctgggcc
     1681 tgaaccccgc cagcatgggc gatctgatcc gcgtgccgat gccgccgatg agcgaagagc
     1741 gccgcaagga gatgaccaag atcgtccgct ccgaaggcga gaacgccaaa atcgccatcc
     1801 gcaacctgcg ccgggacgcc aacgacagca tcaagcgtct ggtcaaagac aaggaagcga
     1861 ccgaggacga ccagaagcgc tccgagaccg aaatccagaa ggccaccgac aagcacatcg
     1921 ccgagatcga tgcgctggtg gtgtcgaagg aaaaggacat catggaggt
//
LOCUS       DAOMVO010000407         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_16992_length_1966_cov_14.226060, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000407 DAOMVO010000000
VERSION     DAOMVO010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16992_length_1966_cov_14.226060"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /gene="phaC"
                     /locus_tag="PKV30_13405"
     CDS             complement(<1..1527)
                     /gene="phaC"
                     /locus_tag="PKV30_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRL31249.1"
                     /translation="MAGNIPQSMLGGPMGQGFAETWMKAFQQFQNMDLGQGAASAAAP
                     PLEFPQLTFAPEALLKLQQDYVQEATALWNQSLQGNPPLKDRRFKGAAWAENPLASFN
                     AAVYLLNARTLMAMAEAAQGDAKTKNRIRFAVEQWMAAAAPSNYLAFNAEAQQKAIDT
                     QGESLAKGLANMLHDAKQGHVSMTDESVFEVGKNVATTEGAVVFENEYFQLIEYKPLT
                     AKVHEKPFLLVPPCINKFYILDLQPENSFIRYAVSQGQRTFVVSWRNPDASMEKKTWD
                     DYIEHAAIEAIHRVQDITGAKQINALGFCVGGTILTTALAVLAARGEKPVASLTLLTT
                     LIDFTDTGILDVFIDENMVRFREMQMGAGGMLKGQDLASTFSFLRPNDLVWNYVVGNY
                     LKGETPPPFDLLYWNSDSTNLPGPFYAWYLRNTYLENKLIQPGKAKVCGEKIDLGKLD
                     LPIYIYGSREDHIVPIGGAYASTQVFPGKKRFMMGASGHIAGVINPPAAKKRSHWVRD
                     D"
     gene            complement(1728..>1966)
                     /locus_tag="PKV30_13410"
     CDS             complement(1728..>1966)
                     /locus_tag="PKV30_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HRL31250.1"
                     /translation="TDAGAAACFTPHAVGKYLADLAGLARRRLAAAGVTHVYGNDGSA
                     DWCTVQQAARYFSYRRDQVPRGGTGRMAACIWLG"
BASE COUNT          294 a          601 c          678 g          393 t
ORIGIN      
        1 gtcatcgcgc acccagtggc tgcgcttttt ggcggccggc gggttgatga cgccggcgat
       61 gtggccggag gcacccatca tgaagcgctt cttgcccgga aacacctgcg tgctggcgta
      121 ggcgccgcca atcggcacga tgtggtcttc gcgcgagccg tagatgtaga tcggcaaatc
      181 gagcttgccg aggtcgatct tttcgccgca caccttggcc ttgccgggtt ggatcagctt
      241 gttctcaaga taggtgttgc gcaggtacca ggcgtagaac gggccgggta ggttggtgga
      301 atcgctgttc cagtacagca gatcgaatgg cggcggcgtt tcgcccttca ggtagttgcc
      361 gacgacgtag ttccacacca ggtcgttggg gcgcaggaag ctgaaggtgc tggccaggtc
      421 ctgccccttg agcatgccgc ccgcgcccat ctgcatctcg cggaaacgca ccatgttctc
      481 gtcgatgaag acgtcgagga tgcccgtgtc ggtgaagtcg atcagggtgg tcagcagcgt
      541 gagcgaggcc acgggctttt cgccccgcgc ggccagcacc gccagcgcgg tggtcaggat
      601 ggtgccgccg acgcaaaagc cgagcgcgtt gatctgcttg gccccagtga tgtcctgcac
      661 gcggtggatc gcctcgatgg cggcgtgctc gatgtagtcg tcccaggtct tcttctccat
      721 cgatgcatcg gggttgcgcc agctgacgac gaaggtgcgc tggccctggc tgacggcgta
      781 gcggatgaag gagttctcgg gctgcaggtc gaggatgtag aacttgttga tgcacggcgg
      841 caccagcaag aacggctttt cgtgcacctt ggccgtcagc ggcttgtatt cgatgagctg
      901 gaaatactcg ttctcgaaca ccaccgcgcc ttcggtggtg gcgacgtttt tgccgacttc
      961 gaacacgctc tcgtcggtca tagacacgtg gccttgcttg gcgtcgtgca gcatgttggc
     1021 cagacccttg gccaggcttt cgccttgcgt gtcgatggct ttttgttgcg cctcggcgtt
     1081 gaaggcgagg tagttgctgg gcgcggcggc ggccatccac tgctcgacgg caaagcggat
     1141 gcggttcttg gtcttggcat cgccctgggc ggcttcggcc atggccatca gcgtgcgcgc
     1201 gttcagcagg tagacggcgg cgttgaagga ggccagcggg ttctcggccc aggcagcgcc
     1261 cttgaagcgg cggtctttga gcggcgggtt gccttgcagg ctctggttcc acagcgcggt
     1321 ggcttcctgc acgtagtcct gctgcagctt tagcagcgcc tcgggcgcga aagtcagttg
     1381 cggaaattca agcggcggcg cggcggcaga ggcggcgccc tgacccaaat ccatgttctg
     1441 gaactgctga aacgccttca tccacgtctc ggcaaagcct tggcccatgg ggccgccaag
     1501 catggattgg ggaatgttgc cggccatctc ctgccacagg gtggtcgcct ggttcatgct
     1561 gctgtctcct tcgttgtttt gccgctcgac ccgggtgggt gggccgctct gggcaccgat
     1621 ccgagtatgc gtgggccgcg tgacacgcga ctgacacggc gccaatatac gccgactttt
     1681 ctgcccgtcg cagccggcgg gtcatggccc gcgccgcagc cccggggtca gcccagccag
     1741 atacaggccg ccatgcgccc ggtgccaccg cgcgggacct gatcgcggcg ataggagaaa
     1801 tagcgcgccg cctgctgcac cgtgcaccag tcggcgctgc cgtcgttgcc gtagacgtgc
     1861 gtgacgcccg ccgccgccag ccgccgccgt gccagcccgg ccaagtcggc caggtatttg
     1921 ccgaccgcgt gcggtgtgaa gcaggcggcg gcgccggcgt cggtgg
//
LOCUS       DAOMVO010000408         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17091_length_1957_cov_16.371188, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000408 DAOMVO010000000
VERSION     DAOMVO010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17091_length_1957_cov_16.371188"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..352
                     /locus_tag="PKV30_13415"
     CDS             65..352
                     /locus_tag="PKV30_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31251.1"
                     /translation="MPCNDTIEEYLLPQIEALLAGTLALMTALAQANAKCTHRELMAA
                     KVRDNLMQLAMHPQLSGTLRTVLARMVGQWAPVAVPVRERAAWHAGTATVQ"
     gene            366..782
                     /locus_tag="PKV30_13420"
     CDS             366..782
                     /locus_tag="PKV30_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31252.1"
                     /translation="MNLIHEHACLLRRLAALQARVSEQMGALARERAAACARADAAER
                     ACQQLQGTLVRERARLVIASTRWWWGLGGPVPDAVRAASPEVTASPMSAADVLCQTGC
                     QGHAHPWLTEDGRCLLKGGDCTRTEGAMVSEAPGAR"
     gene            complement(798..1868)
                     /locus_tag="PKV30_13425"
     CDS             complement(798..1868)
                     /locus_tag="PKV30_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL31253.1"
                     /translation="MPTTAPPVEADAVIIGAGPVGLFQVFELGLLEIRAHIIDALPQV
                     GGQPSELYPGKPIYDIPAIPVCSGQELVDALMQQIAPFEPTFHLNHEVSRFERQADGR
                     FLLETAQGLRLLAKTVFIAAGMGAFQPRRLSVPGIEAFEGTQLFYRVARPAEFAGQRV
                     VIAGGGDSALDWAIHFATEREHAPESVVLLHRRDGFQAAPATVARMRALCAEQRMQLV
                     VGQVTDFDADNGRMTAVKVTGPDGVTRNLALDALLVFHGMSPKLGPIADWQLAIERKQ
                     LVVNTETYGTSEPGIFAVGDINTYPGKKKLILCGFHEATLAAYGALPFVFPGKDVFFQ
                     YTTTSTKLHKVLGVAPAENQAE"
BASE COUNT          327 a          656 c          671 g          303 t
ORIGIN      
        1 atatactgac ctgagccgcg ccactgtcgg cgcgcagacc tgtcgagaca aaggagccgt
       61 cttcatgcct tgcaacgaca ccatcgaaga atacctgctg ccccagatcg aagctttgct
      121 ggccggcaca ctggcgctga tgacggcgct ggcgcaggcc aacgcgaaat gcacgcaccg
      181 cgagctgatg gcggccaagg tgcgcgacaa cctgatgcag ctggcgatgc acccgcagct
      241 ctcgggcacg ctgcgcaccg tgctggcgcg catggtcggc cagtgggcgc cggtggccgt
      301 gcccgtgcgc gagcgcgccg cgtggcacgc cggcaccgcc accgtgcaat gaggcgggtt
      361 cggccatgaa cctgatccac gaacacgcct gcctgctgcg ccgcctggct gcgttgcagg
      421 cgcgggtgag cgagcagatg ggcgcgctgg cgcgcgaacg cgccgcggcc tgcgcgcgcg
      481 ccgacgcggc cgaacgcgcg tgccagcagc tgcagggcac gctggtgcgt gagcgggcgc
      541 gcctggtgat cgcatcgacg cgctggtggt ggggcctggg cgggccggtg cccgatgcgg
      601 tgcgcgcggc gtcgcctgaa gtgaccgcgt cgcccatgag cgccgccgac gtgctgtgcc
      661 agacgggttg tcaaggccac gcgcacccgt ggctgacgga agacggccgt tgcctgctga
      721 aaggcggcga ctgcacgcgc accgagggtg ctatggtttc agaagcgcct ggcgcacgct
      781 gatcaagcgc tgcagcccta ttcggcttga ttttctgccg gcgccacgcc cagcaccttg
      841 tgcagcttgg tgctggtggt ggtgtactga aaaaacacgt ccttgcccgg aaacacgaag
      901 ggcagcgcgc cgtaagcggc cagcgtggct tcatgaaagc cgcacaggat cagcttcttc
      961 ttgcccgggt aggtgttgat gtcgccgacg gcaaagatgc cgggctcgct ggtgccgtag
     1021 gtctcggtgt tcaccaccag ctgcttgcgc tcgatggcga gttgccagtc ggcaatcggc
     1081 cccagcttgg ggctcatgcc gtgaaagacc agcagcgcgt ccagcgccag gttgcgcgtc
     1141 acgccgtctg gccccgtcac cttgaccgcc gtcatgcggc cgttgtcggc gtcgaaatcc
     1201 gtcacctggc cgaccaccaa ctgcatgcgc tgctcggcgc acagcgcacg catgcgcgcc
     1261 acggtggcgg gcgcggcctg aaagccgtcg cgccggtgca gcagcaccac gctttcggga
     1321 gcgtgctcgc gctcggtggc aaagtggatc gcccagtcga gcgccgaatc gccgccgccc
     1381 gcgatcacca cgcgctggcc ggcaaattcg gccggccgcg ccacgcggta gaaaagctgc
     1441 gtgccctcaa acgcctcgat gcccggcacg ctgagccggc gcggctgaaa cgcgcccatg
     1501 cccgcggcaa tgaacacggt cttggccagc aggcgcaggc cttgcgcggt ctcgagcaga
     1561 aagcggccat cggcctgccg ctcgaagcgg ctcacctcgt gattcagatg aaaagtgggc
     1621 tcaaaaggtg cgatctgctg catcagcgca tcgaccagct cttgcccgct gcacaccgga
     1681 atggccggaa tgtcgtagat cggcttgccc gggtacagct cggacggctg gccgccgacc
     1741 tgcggcagcg cgtcgatgat gtgcgcccga atctccagca aacccagctc gaacacctgg
     1801 aacaagccca ccggcccggc gccgatgatg acggcatcgg cttcgacggg cggggcggtg
     1861 gtgggcatcg gttgctacaa aaacaaaagc ggcttgcgca agtggggcct gcgcagaacc
     1921 gcctttttat ttcaatggcg gcgcttcagc gcttcag
//
LOCUS       DAOMVO010000409         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17092_length_1957_cov_12.541009, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000409 DAOMVO010000000
VERSION     DAOMVO010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17092_length_1957_cov_12.541009"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..908
                     /locus_tag="PKV30_13430"
     CDS             258..908
                     /locus_tag="PKV30_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL31254.1"
                     /translation="MTATDNPSPLLTLRGLKRLHVGPVDLALSAGECVSVMGHSGAGK
                     SVLLRMVADLDPHEGDAFLAGGACSGMPAPAWRRQVGYVPADAGWWAETVAPHFPPDF
                     DFAVLLPAVGIEAAARDWPVARLSTGERQRLALLRAMRPAVRVLLLDEPTSGLDPDAV
                     AQVEALLCAHLARGGGLLLVTHDARQATRLAQRHFFIEDGQLRPHDLSSPNPGAAS"
     gene            905..1708
                     /gene="fetB"
                     /locus_tag="PKV30_13435"
     CDS             905..1708
                     /gene="fetB"
                     /locus_tag="PKV30_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HRL31255.1"
                     /translation="MTPPAISALDLALSATLLLLNAGLSLLLGLGLARSLLIAALRTV
                     VQLLLVGMVLKTVFALKSPWLIGAVVLFMGVSAAIEINARQQRRLRGAWSFGIGAGTA
                     TLAAVLAASLGVSLLGAQPWWEPHVVIPMAGIVLGNVMNGVSVTLNAFTSGLTRERAA
                     IEAQLELGATRLQALSGLLQSAIKSGMIPIINQMSAAGIITLPGMMTGQILAGMLPLE
                     AAKYQILVLLLLAGGAGLGAIGAAYAALWRVTDERDRLRLDRLAPHQAE"
     gene            complement(1773..>1957)
                     /locus_tag="PKV30_13440"
     CDS             complement(1773..>1957)
                     /locus_tag="PKV30_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL31256.1"
                     /translation="PGTKIICMGNLAQIDTPYLTEGSSGLTYAVDRFKGWPHGGHVTL
                     ARGERSRLADFASEVL"
BASE COUNT          276 a          646 c          695 g          340 t
ORIGIN      
        1 gccatctgct ccaaactggg ccgcatcccc accgtggccg agtaccagga ggcgatgggc
       61 atcatcgacc aggacaagga cagcgtgtac cgctacatga acttcgacca gattgaggaa
      121 tacgcggacg tggccaagga agtaacggcc tgagtcacgc ctgacccggc cgcgcgtgca
      181 ttcgggtcac tttgaatttg atagctgcca gcgctgatcc atcaagcgct ggtggccttt
      241 tttttgtcaa atcgcggatg acggccaccg acaacccctc gcccttgctg acgctgcgcg
      301 gcctcaagcg cctgcacgtc ggcccggtgg atttggcgct gtcggcgggc gaatgcgtgt
      361 cggtcatggg tcattcgggc gccggcaaga gcgtgctgtt gcgcatggtg gccgacctcg
      421 acccgcacga gggcgacgca ttccttgccg gcggggcctg cagcggcatg ccagcgcccg
      481 cgtggcgtcg ccaggtcggt tacgtgccgg ccgatgccgg ctggtgggcc gagaccgtgg
      541 cgccgcattt cccgcccgat ttcgactttg ccgtgctgct gccggccgtg ggcatcgagg
      601 ccgccgcgcg cgactggccg gtggcgcgcc tgtccaccgg cgagcgccag cggctggcgc
      661 tgctgcgcgc gatgcggccg gcggtgcgcg tgctgttgct ggacgagccg acatcgggcc
      721 tcgaccccga cgccgtggcg caggttgaag cgctgctgtg cgcgcatctg gcgcgtggcg
      781 gcggactgct gctggtgacg cacgacgcgc gccaggcgac gcggctggcg cagcgccact
      841 ttttcatcga ggacgggcag ttgcgtccgc atgacctgag ctcgccaaat ccgggagccg
      901 cgtcatgacg ccgcccgcca tttccgcgct cgacctggcg ctcagcgcca cgttgctgct
      961 gctcaacgcc gggctgtcgc tgctgctggg cctgggtctc gcacgctcgc tgctcatcgc
     1021 ggcgctgcgc accgtcgtcc agttgctgct ggtcggcatg gtgctgaaga ccgtgttcgc
     1081 gctcaagtcg ccctggctga tcggcgccgt ggtgctgttc atgggggtgt cggccgccat
     1141 cgagatcaac gcgcggcagc agcgccggct gcgcggcgcc tggagcttcg gcatcggcgc
     1201 cggcaccgcc acgctggccg ccgtgctggc ggcatcgctg ggtgtcagcc tgctcggggc
     1261 gcaaccctgg tgggagccgc acgtggtgat tccgatggcg ggcatcgtgc tgggcaacgt
     1321 gatgaacggc gtcagcgtga cgctgaacgc cttcaccagc gggctgacgc gcgagcgcgc
     1381 cgccatcgag gcgcagctcg aactgggcgc cacgcggctg caggcgctga gcgggttgct
     1441 gcagagcgcc atcaagagcg gcatgattcc gatcatcaat cagatgtcgg ccgccggcat
     1501 catcacgctg ccgggcatga tgaccggtca gatcctggcc ggcatgttgc cgctggaggc
     1561 ggccaagtac cagattctgg tgctgttgct gctggccggc ggcgccgggc tgggggccat
     1621 cggcgctgcc tatgccgcgc tgtggcgcgt caccgacgag cgcgatcggc tgcggctgga
     1681 ccggctggcg ccgcaccagg cggaataacg tcaaatatca aattgatagc tgtttgcgct
     1741 tgtcagacaa gcgctggcgg ccgatttgat gctcaaagca cttcgctggc aaagtccgcc
     1801 aggcgcgagc gctcgccgcg cgccaacgtc acatggccgc cgtgcggcca gcccttgaaa
     1861 cggtccaccg cgtaggtcag gccgctggag ccctcggtca gatagggcgt gtcgatctgc
     1921 gccaggttgc ccatgcagat gatcttggtg ccggggc
//
LOCUS       DAOMVO010000410         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17143_length_1952_cov_13.212441, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000410 DAOMVO010000000
VERSION     DAOMVO010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17143_length_1952_cov_13.212441"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKV30_13445"
     CDS             <1..198
                     /locus_tag="PKV30_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRL31257.1"
                     /translation="STLAAISAGAVFMGANTYIGNAPNLMVKAIAEDRGVKMPSFFGY
                     MAWSFGILVPLFVIVTFLFFR"
     gene            complement(318..1184)
                     /gene="kynA"
                     /locus_tag="PKV30_13450"
     CDS             complement(318..1184)
                     /gene="kynA"
                     /locus_tag="PKV30_13450"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HRL31258.1"
                     /translation="MHTAGDDLNPHAGEAIVAAERAQLDFSASMSYGDYLHIDQILSA
                     QHPLSPAHDEMLFIIQHQTSELWMKLLLTELASAIADIRADRLPPAFKKLARASRIME
                     QLVHAWDVLATMTPPEYSAIRPYLGPSSGFQSFQYRCIEFALGNKNAAMLKPHAHKPE
                     RLALVQSFYEAPSLYDEALRLMARRGLSVPTDHLKRDWTQPYEASDAVEAAWLQVYRA
                     PEQYWDLYQLGEKLTDLEDAFRLWRFRHVTTVERIIGFKRGTGGTGGVSYLRKMLDVV
                     LFPELWRLRTDL"
     gene            complement(1242..>1952)
                     /locus_tag="PKV30_13455"
     CDS             complement(1242..>1952)
                     /locus_tag="PKV30_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL31259.1"
                     /translation="GALVVWDLAHSAGAVPVDLTGAGADYAVGCGYKYLNGGPGAPAF
                     AWAHPKHVDRFWQPLAGWWGHAAPFAFTPDYQPAAGITRFQCGTQPIVSLSALDCALD
                     VFAESQKLGGMAALRAKSLALTDLFIELVEQLCPGAFTLVTPREHARRGSQICLTPTD
                     QLPEGSAYAIVQALIARGVVGDFRAGDAQQPDILRLGFTPLYLSYENVLQAAHHLRDV
                     LQNKEYAEPRFNQRHAVT"
BASE COUNT          319 a          612 c          679 g          342 t
ORIGIN      
        1 agcacgctgg ccgccatcag tgccggcgcg gtgttcatgg gcgccaacac ctacatcggc
       61 aacgcgccca acctgatggt gaaagccatc gccgaggacc gcggcgtgaa gatgcccagc
      121 ttcttcggct acatggcctg gagcttcggc attctggtgc ccttgttcgt gatcgtgacc
      181 ttcctgttct tcaggtagcg ggttccgcga accgaaggcc gcggagcagg ccatgcggga
      241 acgccgcgga gcgggcttgg cccggccgct ggcgttgtcc cccgctgggg gaaggcgcgc
      301 cagcgcctca gggggagcta caaatccgtg cgcagccgcc acagctcggg gaaaagcacc
      361 acgtccagca tcttgcgcag atagctcacg ccgccggtgc cgccggtgcc gcgtttgaag
      421 ccgatgatgc gttccaccgt ggtcacgtgg cggaagcgcc agaggcggaa ggcgtcttcg
      481 aggtcggtga gtttttcgcc cagttggtac aggtcccaat actgctcggg cgcgcggtag
      541 acctgcagcc acgcggcttc aacggcgtcg ctggcttcat agggttgggt ccagtcgcgc
      601 ttcagatgat cagtcgggac agacaatccg cgccgcgcca tcaggcgcag ggcttcgtcg
      661 tagagcgagg gcgcttcgta aaacgactgc accagcgcca gccgctcggg cttgtgcgcg
      721 tgaggtttca acatcgccgc gttcttgttg cccagcgcga attcgatgca gcggtattga
      781 aagctctgaa agccgctgga cggccccagg tacgggcgga tggcgctgta ttcgggcggc
      841 gtcatcgtcg ccagcacgtc ccaggcgtgc accagctgct ccatgatgcg gctggcgcgc
      901 gccagcttct tgaaagcggg cggcaggcgg tcggcgcgga tgtcggcgat ggcggaagcc
      961 aactcggtca gcagcagctt catccacagc tcgctggtct ggtgctggat gatgaacagc
     1021 atctcgtcgt gcgcgggcga cagcgggtgc tgcgcactca ggatctggtc gatgtgcaga
     1081 tagtcgccgt agctcatgct ggcgctgaag tcgagctggg cgcgttcggc ggccacgatg
     1141 gcttcgccgg cgtgcgggtt caagtcatcg ccagcggtgt gcatggggca agcgggcgcg
     1201 gtcatgggtg tgtcctgttt gttgtttagt cttgatccgg ctcaggtcac ggcgtgccgt
     1261 tggttgaagc gcggctcggc gtactccttg ttttgcagca cgtcgcgcag gtggtgagcg
     1321 gcttgcagca cgttttcgta cgaaagatag agcggcgtaa agcccagacg caggatgtcc
     1381 ggctgctgcg cgtcaccggc gcggaagtcg cccaccacgc cacgcgcaat cagcgcctgc
     1441 acgatggcgt aggcgctgcc ttcgggcagt tgatcggtcg gcgtcaggca gatctgcgag
     1501 ccgcgccgtg catgttcgcg tggcgtgacc agcgtgaaag cgccggggca aagttgctcg
     1561 accagttcga tgaacaaatc cgtcagcgcc agcgacttgg cgcgcagcgc ggccatgcca
     1621 ccgagctttt ggctttcagc aaacacatcc agcgcgcaat ccagcgccga caggctgacg
     1681 atgggctgcg tgccgcactg aaagcgcgtg atgccggcgg ccggctggta gtcgggcgtg
     1741 aaagcgaatg gcgctgcgtg cccccaccag ccggccagcg gctgccagaa gcggtcgaca
     1801 tgcttcgggt gcgcccacgc gaaggccggc gcgccggggc cgccgttcag atacttgtag
     1861 ccgcaaccca cggcgtagtc ggcgccggcg ccagtcaaat cgacgggcac cgcgcccgcg
     1921 ctgtgcgcca ggtcccacac caccagcgcg cc
//
LOCUS       DAOMVO010000411         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17151_length_1951_cov_12.599684, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000411 DAOMVO010000000
VERSION     DAOMVO010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17151_length_1951_cov_12.599684"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1065)
                     /locus_tag="PKV30_13460"
     CDS             complement(70..1065)
                     /locus_tag="PKV30_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRL31260.1"
                     /translation="MNHLPPVSVLRRVARLLVLGVACGVAALAQAQGRYPERTVKVVV
                     ALPAGGSVGAVARLVSQKLADDLGQPFVVDNKAGASGQIGMPQVARAAPDGYTVAVSP
                     ASFLTTNKSIFKSLPYDPEADFAPVAKLVNQSMVLVVRDKSRLGTLTEVLSAARANPG
                     KLTYASSGDGSPQHLAGLLFESRAKVKLLHVPYKGGAPAITDLMAGTVDMVFAPLPEA
                     LPHIKAGKLHPVGVLSEKRSPILPDVTTLREGGVDGLMLSAWIGVLAPARTPPAIVER
                     LNKAVRAVLTGDARAKLVDLGMEPALDDPKSLKQTISDDIRIHAELVRAAGLVPQ"
     gene            1224..>1951
                     /locus_tag="PKV30_13465"
     CDS             1224..>1951
                     /locus_tag="PKV30_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL31261.1"
                     /translation="MSDATALPRPLAYGCLALSMALVGSYVALSKPLALIFPVLLLAW
                     LRFGIGGVAMLHWLKKPASEPPLTPRTRRLLFLESFLGNFLFTVCMITGVKLTSAVSA
                     GVILAAIPAAVAVLSWLFLRERVAPRVWAGVACAVVGIALVSLFKNELPALDQSAPGA
                     QNTSQVAWLGNLLLIGAVLCEASYAVIGKALTGQLSPRRISALINLWGFALSTPFGVW
                     LAWRFDFGAVSAGTWGLLVAYALAA"
BASE COUNT          276 a          665 c          669 g          341 t
ORIGIN      
        1 agcggcgtgt tcatggtcag cgcagccctt gtttcgccgc aacgtggtgc gcatcggagc
       61 cccaccgctt cattgcggca ccagtccggc tgccctgacc agttcggcgt ggatgcgaat
      121 gtcgtcgctg atggtctgct tgagcgactt cggatcgtcc agcgccggct ccatgcccag
      181 atccaccagc ttggcgcgcg catcgcccgt caacacggcg cgcacggcct tgttcaggcg
      241 ctccacgatg gccggcggcg tgcgtgcggg ggccagcacg ccgatccagg ccgacagcat
      301 taggccatcg acaccgccct cgcgcagcgt cgtcacgtcg ggcaggatgg gcgaacgctt
      361 ctcgctcagc acgcccaccg ggtggagctt gccggccttg atgtggggca gcgcctcggg
      421 cagcggcgcg aacaccatgt ccaccgtgcc ggccatcagg tcggtgatgg ccggcgcgcc
      481 gcccttgtag ggcacgtgca gcagtttcac cttggcgcgg ctctcgaaca gcagccccgc
      541 caggtgctgc gggctgccgt cacccgacga ggcgtaggtc agcttgcccg ggttggcgcg
      601 tgcggcggac agcacctcgg tcagcgtacc caggcggctc ttgtcgcgca ccaccagcac
      661 catcgactgg ttgaccagct tggcgacggg cgcgaagtcg gcctccgggt cgtagggcag
      721 cgacttgaag atgctcttgt tggtggtgag aaaggacgct ggcgacaccg ccacggtgta
      781 gccatcgggc gcggcccggg ccacctgggg catgccgatc tggcccgagg cgccggcctt
      841 gttgtcgacc acgaaaggct ggcccaggtc gtcggccagc ttctggctga ccaggcgcgc
      901 caccgcaccg acgctgccgc cggccggcag cgcgaccacc accttgaccg tgcgctcggg
      961 atagcggccc tgcgcctgcg ccagcgcggc gacgccgcag gcgaccccca gcaccagcag
     1021 gcgcgcgaca cggcgcagaa cggaaacagg gggcaaatgg ttcatgaaga ctacgggacc
     1081 aggcgttgct gacaatgcgg cgcaatgggc gccgcgcatg ccctgcgaca agcattattc
     1141 atgccgcatc gctggccggc cagcaccgcg tggcacccgg acgggcgcca caccgtatag
     1201 tcgccacgcc acacgcacaa cccatgtccg acgccaccgc cctgccccgc ccgcttgcct
     1261 atggctgcct ggcgctgtcg atggcgctgg tcggcagtta tgtcgcgctg tccaagccgc
     1321 tggcgctgat ctttccggtg ctcttgctgg cttggttgcg ctttggcatc ggcggcgtgg
     1381 ccatgctgca ctggctgaaa aaaccggcga gcgaaccacc gttgaccccg cgcacgcggc
     1441 gcttgctgtt tctcgaatcg tttctgggca attttctctt cacggtgtgc atgatcaccg
     1501 gcgtcaagct gaccagcgcc gtctcggccg gcgtcattct ggccgccatc cctgccgcgg
     1561 tggcggtgct gagctggctg ttcttgcgcg agcgcgtggc gccgcgtgtg tgggctggcg
     1621 tggcgtgcgc ggtggtcggc atcgcgctgg tgtcgctatt taaaaatgag ctgcccgcgc
     1681 ttgaccagtc tgcgccaggg gcgcaaaaca cttcacaagt cgcatggctg ggcaacctgc
     1741 tgctgatcgg cgccgtgctg tgcgaagcca gctacgcggt catcggcaag gcgttgacgg
     1801 ggcagctgtc gccgcgccgc atcagcgcgc tgatcaacct gtggggcttt gcgctcagca
     1861 cgccgtttgg cgtctggctg gcgtggcgtt ttgactttgg cgccgtgagc gccggcacct
     1921 gggggctgct ggtggcgtat gcgctggccg c
//
LOCUS       DAOMVO010000412         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17268_length_1940_cov_17.586207, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000412 DAOMVO010000000
VERSION     DAOMVO010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17268_length_1940_cov_17.586207"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="rnpB"
                     /locus_tag="PKV30_13470"
     ncRNA           complement(<1..272)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV30_13470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            364..1278
                     /gene="cysK"
                     /locus_tag="PKV30_13475"
     CDS             364..1278
                     /gene="cysK"
                     /locus_tag="PKV30_13475"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRL31262.1"
                     /translation="MKANTILETIGRTPHVRIQRIFGPDANVWVKCERANPGGSIKDR
                     IALGMIEDAEKSGKLQPGGTIVEPTSGNTGVGLAMVAAVKGYKLILVMPDSMSVERRR
                     LMLAYGASFDLTPKEKGMKGAIARAEEIAAATPGAWIPQQFENPANIAAHEATAAEIA
                     ADFPDGLDVLISGVGTGGHITACGRALKAKWPNLKVFAVEPTQSAVISGGAPGPHPIQ
                     GLGAGFIPKNLDTGLLDGVIQVEAEAAREYGRRSAREEGILVGISSGAALAAVAQKLP
                     ELPAGSRILCFNYDTGERYLSVEGYLPA"
     gene            1462..>1940
                     /locus_tag="PKV30_13480"
     CDS             1462..>1940
                     /locus_tag="PKV30_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31263.1"
                     /translation="MTYIKTELGLTALKDRHAVALSRGQRSALILFDGIRSAGAVLAA
                     TGALGVSKADIDTLVSQGLLQAAPNSKPEAARPPQDAPQTDFASSTLAAHYADMELPV
                     APNTPEERMRRYQKAYPLATQITAKLGLRGFRLNLAVESAQGFEGLVALLPKMRAAVG
                     "
BASE COUNT          318 a          671 c          633 g          318 t
ORIGIN      
        1 caacgtgacc gccatcaatc tgggccgggg gtcgcccacc cggctcggtg ccacctaccc
       61 gccagctcag cgggccgcct catcgctggc ctacttggtg ttgctgcgcg cagagattgc
      121 ccgtttcacc ctaactcaat cggctcgtct ctgttgctct gatcctcacc tcgcggtgga
      181 gagccgttag ctcctgcgct gccctgtgca gtccggacgt tcctccagtg cgtggtttcc
      241 caacttgcac cagcggcggt ctggcgggct tcacgcgggc gattatcggc gcatatccat
      301 atgcgcgttg atgtcgaacg cttgcgccag aatgcttcac attccaaccg agcggagacg
      361 cgcatgaaag ccaacaccat tctcgagacc atcggccgca cgccgcacgt gcgcattcag
      421 cgcatcttcg ggcccgacgc caacgtgtgg gtcaagtgcg agcgcgccaa cccgggcggc
      481 tccatcaagg accgcatcgc gctgggcatg atcgaggacg ccgagaagtc cggcaagctc
      541 caacccggcg gcaccatcgt cgagccgacc agcggcaaca ccggcgtcgg cctggccatg
      601 gtggcggccg tcaagggcta caagttgatt ctggtgatgc ccgacagcat gagcgtggag
      661 cgccgccgcc tgatgctggc ctacggcgct tcgttcgacc tgacgccgaa ggaaaaaggc
      721 atgaagggcg ccatcgcccg cgccgaggag attgccgcgg ccacgcccgg cgcatggatt
      781 ccgcagcagt tcgagaaccc ggccaacatc gccgcgcacg aggccacggc ggccgagatc
      841 gcggccgact tccccgatgg gctggacgtg ctgatctccg gcgtcggcac cggcggccac
      901 atcaccgcct gcggccgcgc gctgaaggcc aagtggccga atctgaaggt gtttgccgtc
      961 gagccgacgc aatcggccgt catcagcggc ggcgcgccgg gtccgcaccc gattcagggg
     1021 ctgggcgccg gcttcatccc gaaaaacctg gacaccgggc tgctcgacgg cgtgatccag
     1081 gtcgaggccg aggcggcgcg cgaatacggc cgccgcagcg cgcgcgagga aggcatcctg
     1141 gtcggcatca gctccggcgc cgcgctggcg gcggtggcgc aaaagctgcc cgagctgcct
     1201 gcgggcagcc gcatcttgtg ctttaactac gacacgggcg agcggtatct gtcggtggaa
     1261 gggtatttgc cggcttgagg cgatggcggc gcgcagcgct gctggcatcc cgcctcttcc
     1321 accccgcgcc ggcccgggcg ccgctggaag cgcaactatt tctcattgca gcgccggctc
     1381 ggggtcagcc tctattgatc gccggcgccg atggtgcggc aatcaacgaa ttctttaatc
     1441 ttcgtttgtc tgcgcgcttt catgacttac atcaagaccg aactcggtct gacggctttg
     1501 aaggatcggc acgccgtggc attgagccgc ggtcagcgtt cggcgctgat cctgttcgac
     1561 ggcattcggt cggccggggc cgtcttggcc gccactggcg cgctcggcgt cagcaaggcc
     1621 gacatcgata cgctggtcag ccaaggcctc ttgcaggcgg cgcccaacag caaacccgaa
     1681 gccgcccggc caccgcagga cgccccgcaa accgacttcg cctccagcac gctggccgcg
     1741 cactacgccg acatggagct gccggtggcg cccaacacgc ccgaggagcg catgcgccgc
     1801 taccagaagg cctatccgct ggcgacgcag atcacggcca agctgggctt gcgtggattc
     1861 cgcctcaatc tggcggttga atcggcgcag ggcttcgagg gcctggtcgc cctgctgccc
     1921 aagatgcgcg ccgcggtggg
//
LOCUS       DAOMVO010000413         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17657_length_1908_cov_10.583918, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000413 DAOMVO010000000
VERSION     DAOMVO010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17657_length_1908_cov_10.583918"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PKV30_13485"
     CDS             <1..434
                     /locus_tag="PKV30_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRL31264.1"
                     /translation="LFVTLDDLYVEGLVHITELGGEYFRFDEVRQELRGERTGIRYAI
                     GTRVRVQVSRVDLDGRRIDFRLVREGDELLSRAMRDKGVAPAGERGQPAKSSAKRAAR
                     QQREADRGAARPSPIQALQSAVKKAAAKGKKSGKPRKTRRS"
     gene            674..1429
                     /locus_tag="PKV30_13490"
     CDS             674..1429
                     /locus_tag="PKV30_13490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL31265.1"
                     /translation="MNNTPRTAIVTGAGSGIGRAAALALLADGWQVALAGRRAEPLQQ
                     VADESGAGARALAVPTDVTDPQAVQALFDRTVSAFGRVDLLFNNAGIGAAAAPLEDLA
                     PEQWRSVVDVNLNGMFWCLQAAFRVMKAQSPKGGRIINNGSISAHAPRPHSIAYTATK
                     HAVAGMTKAAALDGRAHDIAVGQIDIGNAATDMTQRMAAGILQANGQLAEEPRMDVAV
                     VGQSVLYMANLPLSANVLFHTVMATKMPFVGRG"
     gene            1445..>1908
                     /locus_tag="PKV30_13495"
     CDS             1445..>1908
                     /locus_tag="PKV30_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31266.1"
                     /translation="MLHIHYVKTPAGRAEMDARTGSLTSRERQIMLLCSGQRPLTVLM
                     EVFGHSVVRELHELAARGLVQAHRQPPEAVPPPAPAPAAEATSTAAAQRLLQAREFAG
                     LVAGSVGGAAAQALVARQSTITQPADILSYCVEVIELLFRTGDVERVTRVGY"
BASE COUNT          289 a          658 c          681 g          280 t
ORIGIN      
        1 gcctgttcgt gacgctggac gatttgtacg tcgaaggcct ggtgcacatc accgagctgg
       61 gcggcgaata cttccgcttc gacgaagtgc gtcaagagct gcgcggcgag cgcaccggca
      121 ttcgctacgc catcggcacg cgggtgcgcg tgcaggtcag ccgcgtcgat ctggacggcc
      181 gccgcatcga ttttcgcctg gtgcgcgagg gcgacgagct gttgtcgcgc gccatgcgcg
      241 acaagggcgt ggcgccggcg ggcgagcgcg gccagccggc caagtcgtct gccaagcgcg
      301 cggcgcgcca gcagcgcgag gccgaccgcg gcgctgcgcg gccgtcgccg attcaggcgc
      361 tccagtcggc ggtcaagaaa gcggccgcca agggcaagaa gagcggcaag ccgcgcaaga
      421 cacgacggtc atgagctccc cctgtgccgc cttcggcgtc ttcccccgaa gggttacggc
      481 gccagtggcc ggcgcaggtc cggccacggc acccacgcat ggcctgctcc gcggccgtct
      541 gctttttggg cgggtgcgcg accgcggcgc gcaaaatgag ctatccgaac gctgccgagt
      601 gactataatt ttcgtagctg cttgcgcttg atggacaagc gctgcagcct gttttgaatc
      661 aaggaggcgc ccgatgaaca acaccccgcg caccgccatc gtcaccggcg ccggcagcgg
      721 cattggccgc gcggcggcac tggcgttgct ggccgatggc tggcaggtcg ccctggccgg
      781 gcgccgcgcc gagccgctgc agcaggtggc cgatgagagc ggcgccggcg cgcgcgcgtt
      841 ggcggtgccc accgacgtga ccgatccgca ggcggtgcag gcgctgttcg atcgcaccgt
      901 gagcgccttc ggtcgcgtcg acctgctgtt caacaacgcc ggcatcggcg cggcggcggc
      961 gccgctggaa gacctggcgc cggagcagtg gcgcagcgtg gtcgacgtca acctgaacgg
     1021 catgttctgg tgcctgcagg cggccttccg cgtcatgaag gcgcagtccc cgaagggcgg
     1081 ccgcatcatc aacaacggca gcatttcggc gcacgcgccg cgcccgcatt ccatcgccta
     1141 cacggccacc aagcacgccg tcgccggcat gaccaaggcg gcggcgctcg atggccgcgc
     1201 gcacgacatc gctgtcggcc agatcgacat cggcaacgcc gccaccgaca tgacgcagcg
     1261 catggccgcc ggcattctgc aggccaacgg ccagttggcg gaagaaccgc gcatggacgt
     1321 ggcggtggtt ggccagtcgg tgctgtacat ggccaatctg ccgctgtcgg ccaacgtgct
     1381 gttccacacc gtgatggcca ccaagatgcc tttcgtcggc cgtggttgag cgcgtgggtg
     1441 ccgcatgctg cacatccact acgtcaagac gccagcgggc cgcgccgaga tggacgcccg
     1501 caccggctcg ctcacctcgc gtgagcggca gatcatgctg ctgtgcagcg gacagcgccc
     1561 gctgacggtg ctgatggagg tgttcgggca ttcggtggtg cgcgagctgc atgagctggc
     1621 ggcgcgcggc ctggtgcagg cgcaccggca accgcctgaa gccgtaccgc cgccggcccc
     1681 agcgccagcg gccgaggcca cgtcgacggc ggccgcgcag cgtttgctgc aagcgcgcga
     1741 attcgccggc ctcgtggccg gttcggtcgg cggcgccgcg gcccaggcgc tggtggcgcg
     1801 ccagtccacc atcacgcagc cggccgacat cctgagctac tgcgtggaag tgatcgagct
     1861 gctgtttcgc accggcgatg tggagcgcgt cacgcgcgtc ggctacaa
//
LOCUS       DAOMVO010000414         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17710_length_1904_cov_9.130882, whole genome shotgun sequence.
ACCESSION   DAOMVO010000414 DAOMVO010000000
VERSION     DAOMVO010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17710_length_1904_cov_9.130882"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..547)
                     /locus_tag="PKV30_13500"
     CDS             complement(140..547)
                     /locus_tag="PKV30_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRL31267.1"
                     /translation="MLLDTGPWVALLCRDDAQHDWSRVQWAALTEPVMSCEAVVAETC
                     FLLARNGFDPAAPLKMIERGVVRLDFSLAPQIASVRALFERYDNVPASLADACLLRLS
                     ELHEPCKVFTLDRDFQIYRRHGRRAVPVISPWA"
     gene            complement(564..812)
                     /locus_tag="PKV30_13505"
     CDS             complement(564..812)
                     /locus_tag="PKV30_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRL31268.1"
                     /translation="MNTLTVKIPIELTEAVDLLAARTHQSRSELIRQALQHYVQAHQA
                     QPATASLLDAMPDLVGCFAGGPADLSTNPAHLSGFGQR"
     gene            complement(865..>1904)
                     /gene="metG"
                     /locus_tag="PKV30_13510"
     CDS             complement(865..>1904)
                     /gene="metG"
                     /locus_tag="PKV30_13510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRL31269.1"
                     /translation="NPEWLRYYIAAKLSGRNEDIDFNADDFMARVNADLVGKFINIAS
                     RAAGFIAKRFDGRLGVVSADGVALLDALRAALPTITTLYEQRETAKVVREVMALCDKV
                     NAYVDANKPWEIAKQPGQDDRLQDVCSTCIEAFRILTIYLKPMLPKLAADVAFFLMVP
                     PESFDDVKTPLGPGAQIGEYTHLMQRVDIKQLEALFEPPAPAPQALAAMNSEVVATEA
                     VPGGEALAPTITIDDFIKTDLRIAKIVDCQAVEGSTKLLRLTLDAGETGDDGAPKLRN
                     VFSGIASAYKPEDLKGKLTVLVANLAPRKMKFGISEGMVLAASHGDEKANPGIFVLEP
                     TPGAQPGMRVR"
BASE COUNT          307 a          627 c          647 g          323 t
ORIGIN      
        1 cgccatcgcc agcggcagcg ccaccacggc caccgtcagg ccggcggcaa agtccttggc
       61 gaagtgcgcg ctgtcgtagc ctttcagggc gtcgatcagg cgggggcgga acggggcgat
      121 gggcagcatg cggtcagctt cacgcccagg ggctgatgac cggcaccgca cggcggccgt
      181 gtcggcggta gatctggaaa tcgcggtcga gcgtgaacac tttgcaaggc tcgtgcaact
      241 cggacagccg cagcaggcag gcgtcggcca gcgatgcggg cacgttgtcg tagcgctcga
      301 acagcgcacg caccgacgcg atctgcggcg ccaggctgaa atccagccgc accacgccgc
      361 gctcgatcat cttcaggggc gcggccggat cgaagccgtt gcgcgccagc aaaaagcagg
      421 tttcagccac cacggcctcg cagctcatga caggctcggt cagggcagcc cactgcacgc
      481 ggctccagtc gtgctgcgcg tcgtcgcggc acagcagcgc cacccacggg cctgtgtcga
      541 gcagcacctg catcgcgtgc tgatcaacgc tggccgaagc cggacaggtg cgcggggttg
      601 gtcgacaagt cggccgggcc gccggcaaaa cagcccacca gatcgggcat ggcgtcgagc
      661 aggctggcag tggccggctg ggcctggtgc gcctgaacgt agtgttgcag cgcctgccgg
      721 atcagctccg agcgcgactg gtgcgtgcgc gccgccagca ggtcgaccgc ctcggtcagc
      781 tcgatcggga tcttgacggt gagcgtgttc atcgccggat aataccggct gccacatcgt
      841 attacaactg agcgacgcga gtcctcagcg cacccgcatc cccggctgcg cgccgggcgt
      901 cggctccagc acgaagatgc cggggttggc cttttcatcg ccgtggctgg cggccagcac
      961 catgccttcg ctgatgccga acttcatctt gcgcggcgcc agattggcga ccagcaccgt
     1021 cagcttgccc ttcaagtctt ccggcttgta cgcactggcg atgccgctga agacgttgcg
     1081 cagcttcggt gcgccgtcgt cgcccgtctc gcccgcgtcc agcgtcaggc gcagcagctt
     1141 ggtggagcct tccacggcct ggcaatcgac gattttggcg atgcgcaggt cggtcttgat
     1201 gaaatcgtcg atggtgatgg tgggcgccag cgcctcgccg cccggcaccg cctccgtagc
     1261 gactacttcg gaattcatag ctgccagcgc ttgcggggcg ggcgctggcg gctcaaacaa
     1321 tgcctcaagc tgctttatgt cgacgcgctg catcaggtgc gtgtactcgc cgatctgcgc
     1381 gccggggccg agcggcgtct tcacgtcgtc gaagctctcg ggcggcacca tcaggaagaa
     1441 ggccacgtcg gccgccagct ttggaagcat gggcttcagg taaatcgtga ggatgcggaa
     1501 cgcctcgatg caggtgctgc acacgtcctg caggcggtcg tcctggccgg gctgcttggc
     1561 gatttcccac ggcttgttgg cgtcgacgta ggcattgacc ttgtcgcaca gcgccatcac
     1621 ctcgcgcacc accttggccg tctcgcgctg ctcgtacaga gtggtgatgg tgggcagcgc
     1681 ggcgcgcagc gcgtcgagca gcgccacgcc gtcggcgctg accacgccca gccggccgtc
     1741 gaagcgcttg gcgatgaagc cggccgcgcg cgaggcaata ttgatgaact tgcccaccag
     1801 gtcggcgttg acgcgcgcca tgaagtcgtc ggcattgaag tcgatatcct cgttgcggcc
     1861 cgacagcttg gcggcgatgt agtagcgcag ccattcggga ttca
//
LOCUS       DAOMVO010000415         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17728_length_1902_cov_14.433135, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000415 DAOMVO010000000
VERSION     DAOMVO010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17728_length_1902_cov_14.433135"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1372)
                     /locus_tag="PKV30_13515"
     CDS             complement(113..1372)
                     /locus_tag="PKV30_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRL31270.1"
                     /translation="MEFDYSPKVKDMQAKLLAFMDEHIYPNENVFHEEVAANRAKGNA
                     WVPTKIVEELKPKARAAGLWNLFLPKSKRAPEGLSNLEYATLCEIMGRVPWAPEVFNC
                     SAPDTGNMETIERYGSEANKDEWLEPLLKGEIRSAFLMTEPAVASSDATNIECSMRRE
                     GDEYVINGRKWWSSGAGDPRCKVYIVMGKTDPEAPRHSQQSMILVPADAKGITVIRPL
                     TVFGYDDAPHGHMEVELKDVRVPVGNLLKGEGQGFAIAQGRLGPGRIHHCMRSIGAAE
                     YALELMCQRLNTRVAFGKPLSTQGVWRERIAESRCMIEQARLLTLKAAYMMDTVGNKV
                     AQAEIAMIKIVAPNMACQIIDWAMQAHGGAGISGDFPLAYMYAHQRTLRFADGPDEVH
                     RNALAKLELAKHSAAAKAVEMPITRGS"
     gene            complement(1436..>1902)
                     /locus_tag="PKV30_13520"
     CDS             complement(1436..>1902)
                     /locus_tag="PKV30_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRL31271.1"
                     /translation="ASALDSGRVSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLGHP
                     LADFAYHCMAWHIPPGAFRGIGGVDVQSLGIPTEGEYIRRYCERTGFTTPEALAPDWN
                     FYLAYNMFRIAAILQGIAKRVEAGTASSAQAKASGAGARPMAELAWRFAQKA"
BASE COUNT          301 a          608 c          630 g          363 t
ORIGIN      
        1 ccggccatga aaaaagcgca gcgggcccgc cggccggctg cgctggatca ctacacaaaa
       61 cgtagcttct cgcgcttgat ggacaagcgc tagagccact ttttatctca aatcagctgc
      121 cgcgcgtgat cggcatctca accgccttgg cggccgcgct gtgcttggcc agctccagct
      181 tggccagcgc gttgcggtgc acttcgtcgg ggccgtcggc aaagcgcagc gtgcgctggt
      241 gggcgtacat gtaggccagc ggaaagtcgc cgctgatgcc agcgccgccg tgcgcctgca
      301 tggcccagtc gatgatctgg caggccatgt tgggcgccac gatcttgatc atggcgatct
      361 cggcctgcgc caccttgttg cccacggtgt ccatcatgta ggcggccttc agcgtcagca
      421 ggcgggcctg ctcgatcatg cagcgcgatt cggcaatgcg ctcgcgccac acgccttgcg
      481 tggatagcgg cttgccaaag gccacgcgcg tgttcaggcg ctggcacatc aactccagcg
      541 cgtattcggc ggcgccgatg gagcgcatgc agtggtgaat gcggcctggc cccaggcgcc
      601 cttgcgcaat ggcaaagccc tgaccctcac ccttgagcag gttgcccacg ggcacgcgca
      661 catctttcag ctcgacttcc atgtggccgt gcggcgcgtc gtcgtagccg aacacagtca
      721 gcgggcggat cacggtgatg cccttcgcat cagccggcac caggatcatc gactgctgcg
      781 aatggcgcgg cgcctcgggg tcggtcttgc ccatgacgat gtagaccttg cagcgcgggt
      841 cgcccgcgcc cgacgaccac cacttgcgac cgttgatgac gtattcgtcg ccctcgcggc
      901 gcatcgagca ttcgatgttg gtggcgtcgg acgaggccac ggccggctcg gtcatcaaaa
      961 aggccgagcg gatttcgccc ttcaacagcg gctccagcca ttcgtccttg ttggcctcgg
     1021 agccgtagcg ctcgatggtc tccatgttgc cggtgtcggg cgccgagcag ttgaagactt
     1081 cgggcgccca gggcacgcgg cccatgatct cgcacagcgt ggcgtattcg aggttgctga
     1141 ggccttcggg cgcgcgcttg gacttgggca ggaacaggtt ccaaaggccc gccgcacgcg
     1201 ccttgggctt cagctcttca actatttttg tcggcaccca cgcgttgccc ttggcgcggt
     1261 tggcggccac ttcttcatgg aacacgttct cgttcgggta gatgtgctcg tccatgaagg
     1321 ccagcagctt ggcctgcatg tccttgacct tggggctgta gtcgaattcc atcgtggctc
     1381 ctttgaaaat ccaaacaaaa tgggccgcca gcgcttgtca gtcaagcgct ggcagctatg
     1441 ctttttgcgc gaaacgccaa gccagctcgg ccatgggcct ggcgcccgcg ccgctggcct
     1501 tggcctgcgc gctgctggcg gtgccggcct cgacccgctt ggcaatgcct tgcaggatgg
     1561 cggcgatgcg gaacatgttg taggcgaggt agaagttcca gtcgggcgcc agcgcctcgg
     1621 gcgtggtgaa tccggtgcgc tcgcagtagc ggcggatgta ttcgccttcg gtcgggatgc
     1681 ccaggctttg cacgtcgacg ccgccaatgc cgcgaaacgc gccgggcgga atgtgccagg
     1741 ccatgcagtg gtaggcgaag tcggccagcg ggtggccgag cgtggacagt tcccagtcaa
     1801 gcaccgccag cacgcgcggc tcggtggggt ggaacatcag gttgtcgagc cggtagtcgc
     1861 cgtgcacgat gctgacgcgg cccgaatcga gcgcgctggc cg
//
LOCUS       DAOMVO010000416         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17772_length_1899_cov_14.084599, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000416 DAOMVO010000000
VERSION     DAOMVO010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17772_length_1899_cov_14.084599"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKV30_13525"
     CDS             complement(<1..413)
                     /locus_tag="PKV30_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRL31272.1"
                     /translation="MLTPEQLVASQKANMETLYGLTAKAFEGMEKLVELNLQATKAAL
                     AESANHTQAVLSVKDAQELLALQAGMFQPLAEKAAAYSRHLYDIASGTSAEFSKAFEG
                     KVAEGQAQFAALVDNAAKNAPAGSETAVAMMKSAVA"
     gene            600..1535
                     /locus_tag="PKV30_13530"
     CDS             600..1535
                     /locus_tag="PKV30_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRL31273.1"
                     /translation="MKAFYATQFVLPLPPGHRFPMGKYQRLRDRLAQECPGLVLNQAE
                     PTADADLALAHAPAYIEAVARGTLTPRAQREIGFPWSEAMADRARRSVGASVQAARVA
                     QREGVAANLAGGTHHASADAGSGYCVFNDIAVAARVLQREQMAERQLTRPPRVAVIDL
                     DVHQGNGTASIFGGDASVFTLSLHGEKNFPFRKVAGDLDVGLPDGTGDAAYLDALDLA
                     LVELDSRFRPDAVFYLAGADPHEGDRLGRLKLTYDGLLARDRRVFEWAWARRLPLALS
                     MGGGYGHDIATTVQVQMNTFAIAAQYWRRWQNAAR"
     gene            1532..>1899
                     /locus_tag="PKV30_13535"
     CDS             1532..>1899
                     /locus_tag="PKV30_13535"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL31274.1"
                     /translation="MNPPARPQPEPRAGYRAFRTITTRWMDNDQYGHVNNVVYYSWFD
                     TAVNAHLIEQGVLDTASGTTIGLVVETQCQYFESLAFPQNVEAGIRVAHIGSSSVRYE
                     VGLFAEGAPLTAAKGHFVHV"
BASE COUNT          300 a          621 c          640 g          338 t
ORIGIN      
        1 gccacggcgc tcttcatcat ggcgacggcg gtctcggagc cagcgggggc gttcttggcg
       61 gcgttgtcca ccagggcggc gaactgggcc tggccttcgg caaccttgcc ttcgaaagcc
      121 ttgctgaact cggcgctggt gcccgaagcg atgtcataca ggtgacggct gtaggcagcg
      181 gctttctcgg ccaggggctg gaacatgccc gcttgcaggg ccagcagctc ttgcgcgtct
      241 ttcaccgaca gcacggcttg cgtgtggttg gcggactcgg ccagggcagc cttggtggct
      301 tgcaggttca gctcgaccag cttttccatg ccttcgaaag ccttggcggt caggccgtac
      361 agcgtttcca tgttggcttt ttgcgaagcg acgagttgtt cgggtgtcag catattcatc
      421 tcctggagaa tggtggaaaa aggaaaaggg ttacttcttt aggtagccgc tcctgaacca
      481 cttgttttgt tgcagtgcag catgggacga attttaggcg ctatcggtcc gattgcaagc
      541 caatttggtg cggcgcagca tttttagatg caaggttcta actccttcag tgcgcgctga
      601 tgaaggcttt ctacgcgact cagttcgtgc tgccgctgcc gccggggcac cgctttccga
      661 tgggcaagta ccaacgcctg cgcgaccgac tggcgcagga atgccccggc ctggttctga
      721 atcaggccga acccactgcc gacgcggacc tggcgctggc gcacgcgccg gcctacatcg
      781 aagcggtggc gcgcggcacg ctgacgccgc gtgcgcagcg cgagatcggc tttccgtgga
      841 gcgaggcgat ggccgatcgc gcgcgccgct cggtcggcgc gagcgtgcag gccgcgcgcg
      901 tggcgcagcg cgagggcgtg gcggccaatc tggccggcgg cacgcaccac gccagtgctg
      961 atgcgggcag cgggtattgc gtgttcaacg acatcgccgt ggccgcgcgc gtgctgcagc
     1021 gcgagcagat ggcggagcgc caactcaccc gaccgccgcg cgtggcggtg atcgacctcg
     1081 acgtgcacca gggcaacggc acggccagca tctttggtgg cgacgccagc gtgttcacgt
     1141 tgtcgctgca cggcgagaag aacttcccct tccgcaaggt ggcgggcgac ctggacgtgg
     1201 gcctgcccga cggcaccggc gacgcggcgt atctggacgc gctggatctg gcgctggtcg
     1261 agctggacag ccgctttcgc cccgatgccg tgttctacct ggccggcgcc gacccgcacg
     1321 agggcgaccg cctgggccgc ctgaagctga cttacgacgg cctgctggcg cgcgaccgcc
     1381 gcgtgttcga gtgggcctgg gcgcgccgcc tgccgctggc gctgagcatg ggcggcggct
     1441 acggccacga catcgccacc accgtgcagg tgcagatgaa caccttcgcc atcgccgccc
     1501 agtactggcg ccgctggcag aatgcagcgc gatgaacccg cctgcacgac cccaacccga
     1561 gccgcgcgct ggctaccgcg ccttccgcac catcaccacc cgctggatgg acaacgacca
     1621 gtacggccac gtcaacaacg tcgtctatta ctcgtggttc gacaccgccg tcaacgcgca
     1681 cctgatcgag cagggcgtgc tcgacaccgc cagcggcaca accatcggcc tggtggtgga
     1741 gacgcagtgc cagtacttcg aatcgctggc gtttccgcag aacgtcgagg ccggcatccg
     1801 cgtggcgcac atcggttcca gcagcgtgcg ctacgaggtt ggcctgttcg ccgaaggcgc
     1861 gcccctgacg gcggccaagg gccatttcgt gcatgtcta
//
LOCUS       DAOMVO010000417         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17773_length_1899_cov_14.057484, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000417 DAOMVO010000000
VERSION     DAOMVO010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17773_length_1899_cov_14.057484"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKV30_13540"
     CDS             complement(<1..728)
                     /locus_tag="PKV30_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL31275.1"
                     /translation="MSTTADLVTTLKKELKAAQMTYADLARELGMAESSVKRMLAKGD
                     MPLSRIDAICRALKLDFAEVAQRVADAQPLLKELTAEQERAVVADEKLLLVATNVLSQ
                     WTLEQMTAAYRLTEAEVIKYLAQLDRIGVIELKPLNRYRLKVAKTFRWRPHGPVMQYV
                     REHVVLDYFSGGFDGPAEGLLLVHGSVSRALAPSFLERLQRVAQDFAQQHQMDQKLKD
                     SDREGYTLLLGMRSWEFEAFTRLRR"
     gene            866..1702
                     /locus_tag="PKV30_13545"
     CDS             866..1702
                     /locus_tag="PKV30_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31276.1"
                     /translation="MRLHTAALHHRLGPPAPSPAHATTAERALLLAVAALITLAVLLP
                     AAALPTGYHDFADQRAWLGLPHAIDVLSNLPFAAMGVWGLVWLRRVPPERIGAAQRGL
                     AALFFVGLMATTLCSAIYHLAPDHAGLCIDRLGMSAAFAGLLGLAAADHISARAGVAL
                     AAFVALAAPATALIDGLAGNMTPWAVLQGGALVLLAALAVRRPQPGALGFSIAGVIAL
                     YAAAKALELADAPVFALTQHLISGHSAKHIVAALAAWPVVRALQRAAQAPANGAAALT
                     IR"
BASE COUNT          264 a          678 c          629 g          328 t
ORIGIN      
        1 cgccgcagcc gcgtgaacgc ctcgaactcc cagctgcgca tgcccagcag cagggtgtag
       61 ccctcgcggt ctgaatcttt cagcttctgg tccatctggt gctgctgcgc aaagtcttgc
      121 gccacgcgct gcagccgctc cagaaacgag ggcgcgagcg cacggctgac gctgccgtgc
      181 accagcagca ggccttcggc cgggccgtcg aagccgccgc tgaagtagtc cagcaccacg
      241 tgttcgcgca cgtactgcat caccgggccg tgcgggcgcc agcggaaggt cttggccacc
      301 ttcaggcgat agcggttgag cggtttcagc tcgatcacgc cgatgcggtc cagctgcgcc
      361 aggtacttga tgacctcggc ctcggtcagg cggtaggcgg cggtcatctg ctccagcgtc
      421 cactggctca gcacgttggt ggccaccagc agcagctttt cgtcggccac cacggcgcgt
      481 tcctgctcgg ccgtcagctc tttcaacagc ggctgcgcgt cggccacgcg ctgggcgact
      541 tcggcaaagt cgagcttgag cgcgcggcaa atcgcgtcga tgcgcgacag cggcatgtcg
      601 cccttggcca gcatgcgctt gacgctgctt tcggccatgc ccagctcgcg cgccaggtcg
      661 gcgtaggtca tctgggcggc tttcagctct ttcttgagcg tggtgacgag gtcggcggtg
      721 gtgctcatgg cggctttgga aaaagaatcg ccaaatgata ccgatggttc aggagcgtga
      781 tggcagttgc aaaatcaggc gcatgatggg cgccgtgggg cgacagtggc gacccgattc
      841 atccacgccc caaggccccg tcaccatgcg tttgcacacc gctgcccttc accaccggct
      901 gggcccacct gccccgtcgc cggcccacgc caccaccgcc gaacgcgcgc tgctgctggc
      961 ggtggccgcg ctgatcaccc tggccgtgct gctgccggcc gccgccttgc ccaccggcta
     1021 ccacgacttt gccgatcagc gcgcctggct gggtctgccg cacgccatcg acgtgctgag
     1081 caacctgccc tttgccgcca tgggcgtgtg ggggctggtg tggctgcggc gtgtgccacc
     1141 cgagcgcatc ggcgcggcgc agcgcggcct ggcggcgttg ttcttcgtcg gcttgatggc
     1201 caccacgctg tgctcggcca tctaccacct ggcgcccgac cacgcgggcc tgtgcattga
     1261 ccggctcggc atgagcgcgg cgtttgccgg cctgctgggg ctggcggcgg ccgaccacat
     1321 cagcgcgcgc gccggcgtgg ccttggcggc tttcgtggcg ctggccgcgc cggccacggc
     1381 cttgatcgac ggcttggccg gcaacatgac gccctgggcg gtgctgcaag gcggcgccct
     1441 ggtgctgctg gcggcgttgg ccgtgcggcg gccgcaacct ggcgcgctgg gcttttcgat
     1501 tgccggcgtg atcgcgttat acgccgccgc caaggcgctc gagctggccg atgcgccggt
     1561 cttcgcgctg acgcagcacc tcatctcagg ccacagcgcc aagcacatcg tggccgcgct
     1621 ggccgcctgg cctgtggtgc gggcgctgca acgcgccgcg caagcgcccg ccaacggcgc
     1681 ggctgccctc acaattcgct gacaagaaca aaaaaaagta ttccccgagg agttgttgcc
     1741 atgagccaga accccagcgc cttcgcgccc gatgcggcat tgcccgcgcc cgcggacgaa
     1801 cccgccgcac acgcatcgcc caaccagttt gcgctgctgc gccagcgccg ctttgcgccc
     1861 tttttctgga cgcagtttgc cggcgccgcc aacgacaac
//
LOCUS       DAOMVO010000418         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17804_length_1897_cov_14.976113, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000418 DAOMVO010000000
VERSION     DAOMVO010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17804_length_1897_cov_14.976113"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1153)
                     /gene="pdxA"
                     /locus_tag="PKV30_13550"
     CDS             complement(125..1153)
                     /gene="pdxA"
                     /locus_tag="PKV30_13550"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRL31277.1"
                     /translation="MRLPIVITLGDPAGIGPEIIAKAFRDAPDTVRGCFVAGDVATLR
                     CAAATLQGDSQVSLPVAEIDTPAAALTAPPRCVPVLQVVPRAEPVALGQVSAAAGKMA
                     GDCVFWAARAALRGEVGAIVTAPLHKEALSAAGAPYSLYPGHTELLQAVAAEHLGVPV
                     AELPVRMMLANDELRTVLVSIHVSLRDAIESVTASNILQTLRITHQSVGAALQKTVRI
                     GVAGLNPHAGEGGLFGREEIEAIGPAIAQARAEGIDARGPFAPDTVFMRGRGFKEFDV
                     VVAMYHDQGLIPVKYLGVEQGVNVTLGLPFVRTSPDHGTAFDIAGQGIADPASLLAAV
                     AMARQLSK"
     gene            complement(1404..>1897)
                     /locus_tag="PKV30_13555"
     CDS             complement(1404..>1897)
                     /locus_tag="PKV30_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRL31278.1"
                     /translation="HDINLLAYHLPLDAHPTLGNNAQLGRRLGLVATGRFGEQDLGFI
                     GQRIDGEAFADADVLAWQLEHVLNRPVTYVGQAPVAIEKIAWCTGGAQGYFESAIAAG
                     AQAFITGEISEPQAHLARECGVAFFACGHHATERYGAPALAAHVAAQLGIEHEFIDID
                     NPA"
BASE COUNT          308 a          658 c          637 g          294 t
ORIGIN      
        1 ccgccggaag aagtgacgct gctgcgcgag attcgcgaca gcctgaagcg ctgatccgct
       61 cagccttcgc agctacataa accgtagctc tcagcgcccg tctgccttgc gcgacgggcg
      121 ttttttattt tgaaagctgg cgcgccatgg cgaccgccgc cagcaggctg gcagggtcgg
      181 caatgccctg gccggcgatg tcgaacgccg tgccgtggtc ggggctggtg cgcacaaagg
      241 gcaggcccag tgtcacgttc acgccctgct ccacccccag gtacttcacg gggatcaggc
      301 cctggtcgtg gtacatcgcg accaccacgt cgaactcctt gaaaccccgc ccgcgcatga
      361 acaccgtgtc gggcgcaaac gggccgcgcg cgtcgatgcc ttcggcgcgc gcctgggcga
      421 tggcggggcc gatggcctcg atctcctcgc gcccgaaaag gccgccttcg ccggcgtgcg
      481 ggttcagccc cgccacgcca atgcgcaccg ttttttgtag cgcggcgcca acgctttgat
      541 gcgtgatgcg cagcgtttgc aggatgttgc tcgcagtaac actttcgatg gcatcgcgca
      601 gcgagacatg aatgctcacc agcacggtgc gcaactcgtc gttggccagc atcatgcgca
      661 ccggcagctc ggccaccggc acgcccaggt gctcggcggc cacggcctgc agcagttcgg
      721 tgtggcccgg gtacagcgaa tacggcgcgc cggcggcgga cagcgcttcc ttgtgcagcg
      781 gcgcggtgac gatggcgccc acctcgcccc gcagcgccgc gcgcgcggcc cagaacacgc
      841 agtcgccggc catcttgccg gccgccgcgc tcacctgccc cagcgccacg ggttcggcgc
      901 gcggcaccac ctgaagcacg gggacgcagc gcggcggcgc tgtcagcgcg gcggcgggcg
      961 tgtcgatttc ggccaccggc aggctgactt gcgaatcgcc ctgcagcgtg gcggcggcgc
     1021 agcgcagcgt ggccacgtcg ccggccacga agcagccacg cacggtgtcg ggcgcgtcgc
     1081 gaaacgcctt ggcgatgatc tccggcccga taccggccgg atcgcccaag gtgatgacga
     1141 tcggtagcct catggtcaaa acaggctcca gcgcttgatt atcaagcgcg agcagctatc
     1201 aacagcatag caatcgcatc gcctgaccat catcacagcg caccgcccgc gcgaaagaag
     1261 aggcttcaac agggctgcgc agcaagccca ggatgggccg cgaagcaggc cacgccggaa
     1321 cgccgtggcc ggggtctccc cggccgccag cgttgtcccc cgctggggga aggcggccac
     1381 aggccgactc agggggcgct ttttcatgct ggattgtcaa tgtcgatgaa ctcatgctcg
     1441 atgcccagct gcgccgccac gtgcgccgcc agcgcgggcg cgccgtagcg ctcggtcgcg
     1501 tggtggccgc aggcgaaaaa ggcgacgccg cattcgcgcg ccaggtgcgc ctgcggttcg
     1561 gaaatctcgc cggtgatgaa cgcctgcgca cccgcggcga tggccgattc aaagtagccc
     1621 tgcgcgccgc cggtgcacca agctattttt tcaatagcta ctggcgcttg tccgacgtac
     1681 gtcaccggcc gattcagcac atgttcaagc tgccaggcca gcacatcggc gtcggcaaag
     1741 gcttcgccgt cgatgcgctg gccgatgaag cccagatcct gctcgccgaa gcggccggtg
     1801 gcgaccaggc ccagccgtcg ccccagttgc gcgttgttgc cgagcgtggg gtgcgcgtcc
     1861 agcggcaggt ggtaggccag caggttgatg tcgtgcg
//
LOCUS       DAOMVO010000419         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17805_length_1897_cov_12.410423, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000419 DAOMVO010000000
VERSION     DAOMVO010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17805_length_1897_cov_12.410423"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKV30_13560"
     CDS             complement(<1..1208)
                     /locus_tag="PKV30_13560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRL31279.1"
                     /translation="MNTEEPTGQAPDPTEERPAPQARSRKKVPPAQAEQAQAATENVA
                     GELLETTEGTPADDSPSSLDEAPATPAAEAAAPDTDAPVAIDDVLSGRFDAEADAPAG
                     PPKRVLLPQADTPKLHKVLAQAGLGSRLEMEQLILEGRISVNNQPAHVGQRIQFGDTV
                     KVNGRPIKVRIAPPPARVLAYHKPVGEVVTMDDPQNRPTVFRKLPRLQPGQGKWQSVG
                     RLDLNTEGLLLFTNSGELANRLMHPRFGLEREYAVRVLGALSDDEKQRLLDGVQLEDG
                     PAQFSAIKEEGTGEGANQWYRVTISEGRNREVRRMIESVGHAVSRLIRIRYGAMLLPR
                     GLRRGAWMELHERDIRALGEASGTPERVIRGAPEPRALPQDKPRKARSDRGRGSGPRP
                     NVAGGRPPEEA"
     gene            complement(1289..>1897)
                     /gene="scpB"
                     /locus_tag="PKV30_13565"
     CDS             complement(1289..>1897)
                     /gene="scpB"
                     /locus_tag="PKV30_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRL31280.1"
                     /translation="ELQDDWAQRGVELVQVASGWRFQSRPEMREYLDRLHPEKPPKYT
                     RATLETLAIIAYRQPVTRGDIEDIRGVTVNSQLIKQLEDRGWVEVIGHRETVGRPGLY
                     ATTRQFLDDLGLESLDQLPMLESASEQAPSMFDALGAAPDEVAGAAAEVEASPVVEGV
                     ALADEHAAIEAASLPPEAEAESNGDTAAPQVDAAERPHSRRA"
BASE COUNT          226 a          641 c          680 g          350 t
ORIGIN      
        1 gcctcttcgg gcggacggcc gccggccacg ttggggcgcg ggccgctgcc ccgaccgcgg
       61 tcactgcggg ctttgcgggg tttgtcctgc ggcagcgcgc gcggctcggg cgcgccgcgg
      121 atcacgcgct cgggcgtgcc cgaggcctcg cccaaggcgc ggatgtcgcg ctcgtgcagt
      181 tccatccagg cgccgcgccg cagcccgcgc ggcagcagca tggcgccgta gcggatgcgg
      241 atcagccggc tcaccgcgtg gcccacgctt tcaatcatgc ggcgcacctc acggttgcgc
      301 ccttcgctga tggtcacgcg gtaccactgg ttggcgccct cgcccgtgcc ttcttccttg
      361 atggcgctga attgcgccgg gccgtcttcc agctgcacgc cgtcgagcag gcgctgtttt
      421 tcgtcatcgc tcaaagcgcc cagcacgcgc acggcgtatt cgcgctcgag cccgaagcgc
      481 gggtgcatca ggcggttggc gagctcgccg gagttggtga acagcagcag cccttcggtg
      541 ttcaggtcga gccggccgac cgactgccac ttgccctgcc ccggctgcag gcgcggcagt
      601 ttgcggaaca cggtggggcg gttctgcggg tcgtccatgg tcacgacttc gcccaccggc
      661 ttgtggtacg ccagcacgcg cgccggcggc ggcgcaatgc gcaccttgat cgggcggccg
      721 ttgaccttga ccgtgtcgcc aaactgaatg cgctggccga cgtgcgccgg ctggttgttg
      781 acgctgatgc ggccttcaag gatgagctgc tccatctcca gccgcgagcc caggcccgcc
      841 tgcgccagca ccttgtgcag cttgggcgtg tcggcctgcg gcagcagcac gcgtttgggc
      901 gggccggcgg gcgcgtcggc ctcggcgtcg aagcggccgg agagcacgtc gtcgatggcg
      961 acgggggcgt cggtgtcggg cgcggcggct tccgctgcgg gcgtcgcggg cgcttcgtca
     1021 aggctgcttg ggctgtcgtc tgcgggcgta ccttcggtgg tttccagcaa ttcaccagct
     1081 acgttttcag tagctgcttg cgcttgttcg gcctgcgctg gcggcacttt ttttctggat
     1141 ctggcctgcg gagccggacg ctcttcggtg gggtcgggcg cctgcccagt cggctcttcg
     1201 gtgttcatgg cgtggggggt tccggtggca gggaatgggc ggtgcgtcgg cagcggtgtc
     1261 ttcaggcgca ggggcggcga tggcttgctc aagcgcgtcg tgagtggggg cgctcagcgg
     1321 catcgacctg cggcgcggcg gtgtcgccgt tgctctcggc ttcagcttct ggtggcagcg
     1381 atgcggcttc aatcgcagcg tgctcgtcgg ccagcgcaac accttcaacc accggcgacg
     1441 cttcaacctc agcggccgcg ccagccacct catccggcgc cgcgcccagt gcgtcgaaca
     1501 tgctgggggc ctgctcagac gccgattcca gcatcggcag ctgatccagc gattccagcc
     1561 ccaaatcgtc cagaaactgg cgcgtggtgg cgtacaggcc ggggcggccc acggtctcgc
     1621 ggtggccgat cacctcgacc cagccgcgat cctccaactg cttgatcaac tgcgagttga
     1681 cggtgacgcc gcgaatgtcc tcgatgtcgc cgcgcgtgac cggctggcgg taggcaatga
     1741 tggccagcgt ctccagtgtg gcgcgggtgt acttgggtgg cttttcggga tgcaggcggt
     1801 cgaggtactc gcgcatctcg ggccgacttt gaaagcgcca gccgctggcc acctgcacca
     1861 gctcgacgcc gcgctgcgcc cagtcgtcct gcagctc
//
LOCUS       DAOMVO010000420         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17816_length_1896_cov_21.875068, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000420 DAOMVO010000000
VERSION     DAOMVO010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17816_length_1896_cov_21.875068"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /gene="rplV"
                     /locus_tag="PKV30_13570"
     CDS             <1..239
                     /gene="rplV"
                     /locus_tag="PKV30_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRL31281.1"
                     /translation="ALNTLTFTQKKAAGIIKKVLESAIANAEHNDGADIDELRVKTIY
                     VEQGTTLKRSAARAKGRGARLSKPTCHVYVTVGN"
     gene            258..1163
                     /gene="rpsC"
                     /locus_tag="PKV30_13575"
     CDS             258..1163
                     /gene="rpsC"
                     /locus_tag="PKV30_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRL31282.1"
                     /translation="MGQKIHPTGFRLAVSRNWASRWYASNRDFAGMLAEDIKVREYLK
                     AKLKNAAVSRVLIERPAKNARITIYSARPGVVIGKKGEDIEKLKKDLAAKLGVPVAVN
                     IEEVRKPEVDAQLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNGI
                     EIARTEWYREGRVPLHTLRADIDYGFSEAKTTYGVIGVKVWVYKGDNLGRNDAPSLDS
                     TPRPEGEERRGPRGPRRDGRGGPGGDRAGRGGPRRDARGGSNAAPADGSDKPAEAAGA
                     DAKPAVKRVRQVAAPAAAADGAKGE"
     gene            1167..1583
                     /gene="rplP"
                     /locus_tag="PKV30_13580"
     CDS             1167..1583
                     /gene="rplP"
                     /locus_tag="PKV30_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRL31283.1"
                     /translation="MLQPARRKYRKEQKGRNTGIATRGNTVAFGDFGLKSTDRGRLTA
                     RQIESARRAISRHVKRGGRIWIRVFPDKPISTKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEIVGVPEELAREAFALAAAKLPLRTTFVARMIGQ"
     gene            1598..1810
                     /gene="rpmC"
                     /locus_tag="PKV30_13585"
     CDS             1598..1810
                     /gene="rpmC"
                     /locus_tag="PKV30_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRL31284.1"
                     /translation="MKTAELRNKDVAGLQAEVKDLQKAHFNLRMQKATQQLSNTGQLK
                     ITRRGIARAKTLIAEKQAADKQSAAK"
     gene            1820..>1896
                     /locus_tag="PKV30_13590"
     CDS             1820..>1896
                     /locus_tag="PKV30_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRL31285.1"
                     /translation="MTEAKKSLKRTLIGKVVSDKRAKTVT"
BASE COUNT          417 a          650 c          588 g          241 t
ORIGIN      
        1 aggcgctgaa cacgctcacc ttcacgcaga agaaggccgc cggcatcatc aagaaggtgc
       61 tggaatccgc catcgccaac gccgagcaca acgacggtgc cgacatcgac gagctccgcg
      121 tcaagaccat ctacgtcgag cagggcacca cgctcaagcg ctcggctgcc cgcgccaagg
      181 gccgcggcgc gcgcctgtcg aagcccacgt gccacgtgta cgtgacggtc ggcaactaag
      241 gaaggcctgg aaagactatg ggacagaaaa tccatccgac cggcttccgc ctcgccgtga
      301 gccgcaactg ggccagccgc tggtacgcca gcaaccgcga cttcgccggc atgctggccg
      361 aagacatcaa ggtgcgcgag tacctgaagg ccaagctcaa gaacgccgcc gtctcgcgcg
      421 tgctgatcga gcgccccgcc aagaacgccc gcatcaccat ctactcggca cgtccgggcg
      481 tggtgatcgg caagaagggc gaggacatcg agaagctcaa gaaagacctg gccgccaagc
      541 tgggcgtgcc cgtcgccgtc aacatcgagg aagtgcgcaa gcccgaagtc gatgcccaac
      601 tgatcgccga cagcatcacg cagcagcttg aaaagcgcat catgttccgc cgcgccatga
      661 agcgcgccat gcagaacgcc atgcgtctgg gcgcccaagg catcaagatc atgtccgccg
      721 gccgcctgaa cggcatcgag atcgcgcgca ccgagtggta tcgcgaaggt cgcgtgccgc
      781 tgcacacgct gcgcgccgac atcgactacg gtttcagcga agccaagacc acttacggcg
      841 tcatcggcgt caaggtgtgg gtctacaagg gcgacaacct gggccgcaac gacgcaccgt
      901 cgctggacag cacgccgcgc cccgagggcg aagagcgccg tggtccgcgt ggtccgcgcc
      961 gtgacggccg tggtggcccc ggtggcgacc gcgccgggcg tggcggcccg cgccgtgatg
     1021 cgcgtggtgg cagcaacgcc gcgcctgccg atggcagcga caaacccgct gaggccgcag
     1081 gggccgacgc caaacccgcc gttaagcgcg tccgccaagt cgccgcgcca gctgcagcag
     1141 ctgacggcgc caaaggagag taaaccatgc tgcaacctgc acgcagaaag taccgcaagg
     1201 agcagaaagg ccgcaacacc ggtatcgcca cccgtggcaa taccgtggcc tttggcgact
     1261 tcggtctcaa gtccaccgat cgcggccgcc tgacggcgcg ccagatcgaa tccgcgcgtc
     1321 gcgccatttc gcgccacgtc aagcgcggtg gccgcatctg gatccgtgtg ttcccggaca
     1381 agccgatttc caccaagccc gccgaagtgc gtatgggcaa cggtaaaggc aaccccgagt
     1441 actacgtggc cgaaatccag cccggcaagg tgctgtacga gatcgtcggt gtgcccgaag
     1501 agctggcgcg cgaggcgttt gccctggccg ctgccaagct gccgctgcgc accaccttcg
     1561 ttgcccgcat gatcggccag taatcaggag acaccagatg aaaaccgctg aactgcgtaa
     1621 caaagacgtc gccggcctgc aagccgaggt gaaggacctg caaaaggccc acttcaacct
     1681 gcgcatgcaa aaagccaccc agcagctgtc caacaccggg cagttgaaga tcacgcgccg
     1741 cggcattgcc cgcgccaaga cgctgatcgc cgaaaagcag gctgccgaca agcagtccgc
     1801 cgccaagtaa ggagcgacca tgacggaagc caagaaatcc ctcaagcgca ccctgatcgg
     1861 caaggtcgtc agcgacaagc gcgccaagac cgtgac
//
LOCUS       DAOMVO010000421         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_17852_length_1893_cov_13.328618, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000421 DAOMVO010000000
VERSION     DAOMVO010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17852_length_1893_cov_13.328618"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..668
                     /gene="pth"
                     /locus_tag="PKV30_13595"
     CDS             27..668
                     /gene="pth"
                     /locus_tag="PKV30_13595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRL31286.1"
                     /translation="MIKLFVGLGNPGPEYAATRHNAGFWWIDQMARRLNATLVAERGF
                     HGLVARVNVGGQSVWLLQPQTFMNLSGKSVAALARFYKISPDEILVAHDELDLPAGEA
                     KLKQGGGHAGHNGLRDIHAQLGSSDYWRLRLGIGHPGSKSEVVGWVLKKPPLDEQIGI
                     EQAIDRAAKAFSQLAAGDMAAATQIIHTAKPPRPKPPRKPPPEAAEPPVVDAP"
     gene            complement(750..1001)
                     /locus_tag="PKV30_13600"
     CDS             complement(750..1001)
                     /locus_tag="PKV30_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HRL31287.1"
                     /translation="MALMITDECINCDVCEPECPNEAISMGAEIYQINPDKCTECVGH
                     FDEPQCVQVCPVECIPLDPQHAEDKETLWAKYRRLQAGA"
     gene            1107..>1893
                     /locus_tag="PKV30_13605"
     CDS             1107..>1893
                     /locus_tag="PKV30_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL31288.1"
                     /translation="MSYFLFKRVLTLLATLLATSVIVFAVLEVLPGNAAQVLMGPDAD
                     PAAVAAKATELGLDQPVLRRYAAWIGGLLRGDMGLSYTYGSQVSELIAERLALTIPLA
                     VLAMLLTTAIALLVGVYAASRHNGPGDVGLMTLAQIGIAIPNFWFAILLILLFSVKLQ
                     WLSAGGFDGWYFDDSVWRGLWAGFKALILPAVALAVVQAAILARFTRSAVLEVLREDF
                     VRTARAKGLSHRATLWGHVLRNAMIPVITVMGLQFAELLAGTIV"
BASE COUNT          300 a          633 c          614 g          346 t
ORIGIN      
        1 ttccgacttg ccgataatcc cccgccatga tcaagctgtt tgtcggcctg ggcaacccgg
       61 gcccagagta cgcggccacg cgccacaacg cgggcttctg gtggatcgac caaatggcgc
      121 ggcggttgaa cgccactttg gtggccgagc gcggctttca cggcctggtg gcgcgcgtca
      181 acgtcggcgg ccagagcgtg tggctgctgc agccgcagac cttcatgaac ctgtccggca
      241 agtcggtggc ggcgctggcg cgcttctaca agatctcgcc ggacgaaatt ctggtcgcac
      301 acgacgaact cgacctgccc gccggcgaag ccaagctcaa gcaaggcggc ggccacgccg
      361 ggcacaacgg cctgcgcgac attcacgcgc agctgggctc gtccgactac tggcgcctgc
      421 gccttggaat aggccacccg ggcagcaagt cggaagtggt gggctgggtg ctgaaaaagc
      481 cgccgctgga cgagcagatc ggcatcgagc aagccatcga ccgcgcggcc aaggcgtttt
      541 cgcaactggc tgcgggcgac atggcggcgg ccacgcaaat catccacacc gccaagccac
      601 cccggcccaa gccgccgcgc aaaccgccac ctgaagcggc cgagccgccg gtagtggacg
      661 cgccttgaag cgcgttgtaa aactacaata ttgatagctg ttcgcgcttg tcctgcaagc
      721 gctggcggcc aaaatcattc aaaaccccgt caagcacccg cctgcagtcg ccggtacttg
      781 gcccacagcg tttccttgtc ctcggcgtgc tgcgggtcga gcggaatgca ctccaccgga
      841 cacacctgca cgcactgcgg ctcgtcgaag tggccgacgc attcggtgca cttgtccggg
      901 ttgatctgat agatctcggc gcccatcgag atcgcctcgt tcgggcattc aggttcgcag
      961 acgtcgcagt tgatgcactc gtcggtgatc atcagcgcca tgacaacact ccattggtga
     1021 ggccgccatt atcccgccgc cgcccggcgc acggtctgcg ctggcgcaaa tgccgccagg
     1081 ctttgtgttg ataatgccgc cgacccatga gctactttct gttcaagcgc gtgctgacgc
     1141 tgctggccac cttgctggcg accagcgtca tcgtgttcgc ggtgctcgag gtgttgccgg
     1201 gcaacgccgc gcaggtgctg atgggccccg acgccgaccc cgccgccgtg gccgccaagg
     1261 ccaccgaact gggtctggat caaccggtgc tccgccgcta cgccgcctgg atcggtggcc
     1321 tgctgcgcgg cgacatgggg ctgtcttaca cctacggctc gcaggtgtcc gaattgatcg
     1381 cggagcggct ggcgctgacc attccgctcg ccgtgctggc gatgctgctg accaccgcca
     1441 tcgccctgct ggtgggcgtg tacgccgcct cgcgccacaa cggcccgggc gacgtgggcc
     1501 tgatgacgct ggcgcagatc ggcattgcca tccccaactt ctggttcgcc atcctgctga
     1561 tcctgctgtt ctcggtcaag ctgcagtggc tttcagccgg cggttttgac ggctggtact
     1621 ttgacgatag cgtctggcgc gggttgtggg cgggcttcaa ggcgctgatt ctgccggccg
     1681 tggctttggc cgtggtgcag gcggcgatcc tggcgcgctt cacgcgatcg gcggtgctgg
     1741 aagtgctgcg cgaagacttc gtgcgcaccg cgcgcgccaa gggcttgtcg caccgcgcca
     1801 cgctgtgggg ccacgtgctg cgcaacgcga tgattccggt catcaccgtg atgggcctgc
     1861 agtttgccga gctgctggca ggcaccatcg tga
//
LOCUS       DAOMVO010000422         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18135_length_1872_cov_16.159604, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000422 DAOMVO010000000
VERSION     DAOMVO010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18135_length_1872_cov_16.159604"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="pncB"
                     /locus_tag="PKV30_13610"
     CDS             <1..902
                     /gene="pncB"
                     /locus_tag="PKV30_13610"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRL31289.1"
                     /translation="RGPWLHTIMFEIPVLAIVSEVYFRNTQPGLDLTEGRRRLQDKIA
                     LMKGVHDLKIADYGTRRRFSLEWQREVLQTLQADLGTGPDGQLTGTSNVLFAMELGMR
                     PMGTMAHEYLQACQALGPRLRDSQVFGFEKWAQEYRGDLGIALSDVYGMNAFLRDFDM
                     YFCKLFDGARHDSGDPFQWGERMIEHYQANRVDPRSKVIIFSDGLTIPRCIELHERFR
                     GRIQTAFGVGTNLTNDLGPDALQLVLKMIECNGQPVAKISDTPGKTMVEDEGYLAYLR
                     QVFDLPAPPPTTTPINRSAPVMR"
     gene            986..>1872
                     /locus_tag="PKV30_13615"
     CDS             986..>1872
                     /locus_tag="PKV30_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRL31290.1"
                     /translation="MTLPRVSSLRLATFFRLALLLGVLTAGAPAQAATLDGSQLSVLW
                     AVPFAGILLSIAIWPLAGPHFWHHHFGKIAAAWALAFLVPFALVFGAGTAMAGLVHAL
                     LAEYIPFILLLTALFTVSGGIYIRGNLHGTPLLNTGILLVGGLLASFMGTTGASMLLI
                     RPLIRANDNRQSCAHVVVFFIFIVSNIGGSLTPLGDPPLFLGFLKGVSFFWTVGHILP
                     DTAFLMVTLLALFYVLDSYLYKKQGVTRADPTPDTTRFGFDGSINFALLAVVVALVLM
                     SGVWKPGIEFDIMGTHVGLP"
BASE COUNT          289 a          621 c          595 g          367 t
ORIGIN      
        1 tgcgcggccc ctggctgcac accatcatgt tcgagattcc ggtgctggcc atcgtcagcg
       61 aggtgtactt ccgcaacacg cagccggggc tggacctgac cgagggccgc cgccggctgc
      121 aggacaagat cgcgctgatg aagggcgtgc acgatttgaa gattgccgat tacggcacgc
      181 gccggcgctt ttcgcttgaa tggcagcgcg aggtattgca gaccctgcag gccgatctgg
      241 gcaccggccc cgacggccag ctcaccggca ccagcaacgt gctgtttgcc atggaactgg
      301 gcatgcgccc catgggcacc atggcgcacg aatacctgca ggcctgccag gcgctgggcc
      361 cgcgcctgcg cgacagccag gtgttcggct tcgagaagtg ggcgcaggaa tatcggggtg
      421 acttagggat cgcgctgtcc gacgtgtacg gcatgaacgc ctttttgcgc gatttcgaca
      481 tgtatttctg caagctgttc gacggcgcgc ggcacgattc gggcgacccg tttcagtggg
      541 gcgaacgcat gatcgagcac taccaggcca accgtgtcga tccgcgcagc aaggtcatca
      601 tcttcagcga cggcctgacc attccgcgct gcatcgagct gcacgagcgc tttcgcggcc
      661 gcatccagac cgcctttggc gtcggcacca acctgaccaa cgacctcggc cccgacgcgc
      721 tgcaactggt gttgaagatg atcgaatgca acggccagcc ggtggccaag atctcggaca
      781 cgccgggcaa gaccatggtg gaggacgagg gctacctggc ctacctgcgc caggtgttcg
      841 acctgcccgc accgccgcca accaccacgc ccatcaaccg ctcggcgccg gtgatgcgct
      901 gagcttttgc tctattatta atagctgccc gcgctggcca gtcatgcgcc agagccattt
      961 ttaacttcaa acaccgattc tcctgatgac cctgccgcgc gtttccagcc tccgccttgc
     1021 cacgttcttt cgcctcgcgc tgctgctggg cgtgctgacg gcgggcgcgc cggcgcaggc
     1081 ggccacgttg gacggcagcc agctgtcggt gctgtgggcg gtaccgtttg ccggcatcct
     1141 gctgtcgatc gccatctggc cgctggccgg gccgcatttc tggcaccacc acttcggcaa
     1201 gatcgcggcg gcctgggcgc tggcgtttct ggtgccgttc gcgctggtgt ttggcgccgg
     1261 cacggcgatg gccgggctgg tgcatgcgct gctggccgaa tacatcccct tcatcctgct
     1321 gctgacggcg ctgttcacgg tgtccggcgg catctacatc cgcggcaacc tgcatggcac
     1381 gccgctgctc aacaccggca ttctgctggt cggcgggctg ctggccagct tcatgggtac
     1441 cacgggcgcg tcgatgctgc tgattcgccc gctgatccgt gccaacgaca accgccagag
     1501 ctgcgcgcac gtggtggtct tcttcatctt catcgtcagc aacatcggcg gctcgctgac
     1561 gccgttgggc gatccgccgc tgtttctggg ctttttgaag ggcgtcagct tcttctggac
     1621 ggtgggccac atcctgcccg acaccgcatt tttgatggtc acgctgctgg cgctgttcta
     1681 cgtgctcgac agctacctct acaagaagca gggcgtgacc cgcgccgacc cgacgcccga
     1741 caccacgcgc ttcggctttg acggcagcat caacttcgcg ctgctggcgg tggtggtggc
     1801 gctggtgctg atgagcggcg tgtggaagcc gggcatcgag ttcgacatca tgggcacgca
     1861 cgtcggcctg cc
//
LOCUS       DAOMVO010000423         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18389_length_1852_cov_16.917641, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000423 DAOMVO010000000
VERSION     DAOMVO010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18389_length_1852_cov_16.917641"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /gene="sucA"
                     /locus_tag="PKV30_13620"
     CDS             <1..987
                     /gene="sucA"
                     /locus_tag="PKV30_13620"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRL31291.1"
                     /translation="DCGRGTFVHRHAVLHDQKREKWDVGTYVPLQHVADNQAPFNVID
                     SILSEEAVLGFEYGYASNDPNTLVIWEAQFGDFVNGAQVVIDQFIASGEVKWGRVNGI
                     TLMLPHGYEGQGPEHSSARLERFMQLSADTNMQVVQPTTASQIFHVLRRQMIRPLRKP
                     LVIMTPKSLLRNKDATSPLAEFTKGGFQTVIGDRGDVKADKVKRVIACSGKVYYDLAK
                     RREEKGIDDVAILRVEQLYPFPHKAFAAELKKYPGATEVVWCQDEPQNQGAWFFVQHY
                     IHENMASGQRLGYAGRAASASPAVGYAHLHQDQQKALIDAAFGRLKGFVLQK"
     gene            1083..>1852
                     /locus_tag="PKV30_13625"
     CDS             1083..>1852
                     /locus_tag="PKV30_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRL31292.1"
                     /translation="MAIVEVKVPQLSESVAEATLLQWKKKPGDAVAIDEILIEVETDK
                     VVLEVPAPAAGVLAEIVQPDGATVVADQLLAKIDTEGVAGAAAAPAPAAAAPAAAAPA
                     PAPAAAPAAAPAAAGGAKSDIAMPAAAKLMADNNLAAGSVAGTGKDGRVTKGDVLGAM
                     QSGAAGASQSSAAAAKPAQIPTGAPTSALPQVASPAAPKLGDRPEQRVPMTRLRARVA
                     ERLLQSQSTNAILTTFNEVNMQPVMDMRKRFQEKFEKE"
BASE COUNT          365 a          652 c          570 g          265 t
ORIGIN      
        1 gactgcggcc gcggcacctt cgtgcaccgc cacgccgtgc tgcacgacca gaagcgcgag
       61 aagtgggacg tcggcaccta cgtgccgctg cagcacgtgg ccgacaacca ggcaccgttc
      121 aacgtcatcg attccatcct gtctgaggag gcggtgctgg gcttcgagta cggctacgcg
      181 tccaacgacc ccaacacgct ggtgatctgg gaggcgcagt tcggcgactt cgtcaacggc
      241 gcgcaagtgg tgattgacca gttcatcgcc tcgggcgaag tgaagtgggg ccgcgtcaat
      301 ggcatcacgc tgatgctgcc gcacggctac gagggccagg gccccgagca ctcgtcggcg
      361 cggctcgagc gcttcatgca actcagcgcc gacaccaaca tgcaggtggt gcagcccacc
      421 acggccagcc agatcttcca cgtactgcgc cgccagatga ttcgcccgct gcgcaagccg
      481 ctggtcatca tgacgcccaa gagcctgctg cgcaataaag acgccacctc gccgctggcc
      541 gaattcacca agggcggctt ccagaccgtg atcggcgacc gcggcgacgt gaaggccgac
      601 aaggtcaagc gcgtcattgc ctgctcgggc aaggtctatt acgacctggc caagcggcgc
      661 gaagagaaag gcatcgacga cgtggccatc ctgcgcgtcg aacagctcta cccgttcccg
      721 cacaaggctt tcgcggccga gctgaagaaa tacccgggcg ccaccgaagt cgtgtggtgc
      781 caggacgagc cgcaaaacca gggcgcctgg ttcttcgtgc agcactacat ccacgagaac
      841 atggcttcgg gccagcgcct gggctacgcc ggccgcgccg cgtcggcatc gcccgccgtc
      901 ggctacgcgc acctgcacca ggaccagcaa aaggcgctga tcgatgccgc ctttggccgc
      961 ctgaagggct tcgtgcttca aaaataagag ctgctggcgc ttgatacaca ggcgccagcg
     1021 gcctttttga tgcctaaccc acacgctgaa ccgcactcag aaaagaactg aaagaaattg
     1081 aaatggccat cgttgaagtc aaagtccccc aactgtccga atccgtggcc gaggccacgc
     1141 tgctgcagtg gaagaaaaag cccggcgacg ccgtcgccat cgatgaaatc ctgatcgagg
     1201 tcgagaccga caaggtcgtg cttgaagtgc ccgcgcccgc cgccggcgtg ctggccgaga
     1261 tcgtgcagcc cgacggcgcc accgtggtcg ccgatcagct gctggccaag atcgacaccg
     1321 aaggcgtggc cggcgccgct gcagcgcccg cgcctgccgc cgccgcgccc gctgcggcag
     1381 ccccggcccc tgcacctgct gctgcacctg ctgctgcacc cgctgccgcc ggcggtgcca
     1441 agtccgacat cgccatgccc gccgctgcca agctgatggc cgacaacaac ctggccgccg
     1501 gctctgtcgc cggcaccggc aaggacggcc gcgtgaccaa gggcgacgtg ctgggcgcta
     1561 tgcaatccgg agctgctggc gcaagccaat caagcgctgc ggccgcaaag cccgctcaaa
     1621 tcccgaccgg cgcgccgacc agcgcgctac cgcaggttgc cagccccgcc gcgcccaagc
     1681 tgggcgatcg ccccgaacag cgcgtgccca tgacgcgcct gcgcgcccgg gtggccgagc
     1741 gccttttgca atcgcaatcc accaacgcca tcctcaccac gttcaacgaa gtgaacatgc
     1801 agccggtgat ggacatgcgc aagcgcttcc aggagaagtt cgaaaaagag ca
//
LOCUS       DAOMVO010000424         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18453_length_1847_cov_11.613281, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000424 DAOMVO010000000
VERSION     DAOMVO010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18453_length_1847_cov_11.613281"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="PKV30_13630"
     CDS             <1..107
                     /locus_tag="PKV30_13630"
                     /inference="COORDINATES: protein motif:HMM:NF021366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HRL31293.1"
                     /translation="WRQAFTQLMQMADGQLRFFGLVAVGGGLLLLWLA"
     gene            268..1428
                     /locus_tag="PKV30_13635"
     CDS             268..1428
                     /locus_tag="PKV30_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRL31294.1"
                     /translation="MSAWLLPDHVADVLPSEARHIEELRRRLLDTACTYGYELVIPPL
                     IEHLDSLLTGTGQALELQTFKLVDQLSGRSLGLRADTTPQVARIDAHLLGRQAITRLC
                     YCGPVLHTRPERPHATREPLQFGAEIYGHAGREADVEILSLALDCLRAAPLAGLSVDL
                     ADARIVRALLAGVMAAPTVLAEVHHALAAKDASTLGRLTRDFPAASREGLQALVGLYG
                     DEEVLIEAEKALQRTPGVREALSNLKYLASRLEGVRVSFDLADARGYGYYTGARFAVY
                     AEGAGDAVLRGGRYDGVGAVFGHKLDRERPAVGFSLDVKQLARAAASAPLRAAVRAPW
                     SDAPGLQAAVQALRARGETVVCVLPGSHSQIDEFNCDRELIEAGGQWVIQSL"
     gene            1439..>1847
                     /locus_tag="PKV30_13640"
     CDS             1439..>1847
                     /locus_tag="PKV30_13640"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRL31295.1"
                     /translation="MKPTTGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNA
                     GHTLVINGVKTALHLIPSGIMRPGVTCYIGNGVVLSVSKLLEEIEGLEKAGVQVRERL
                     RISEGCPLILPLHAALDVAREAARESGGSEKIGT"
BASE COUNT          296 a          626 c          644 g          281 t
ORIGIN      
        1 cttggcgcca ggccttcact cagctgatgc agatggcgga cgggcagctg cgctttttcg
       61 gcctggtggc cgttgggggc ggactgctgt tgctctggtt agcgtagcgg ctgagcagca
      121 aaccgcgcag gcccgcggag gagtccacgc cagaacgccg tggccgggct tgcaccggcc
      181 accagcgttg tcccctgggg gaaggcgcgc agcgccacag gggggaccct ttagaatcac
      241 gtttttaaca gcccagcgaa aagcgccatg tccgcctggc tcctgccgga tcacgtcgcc
      301 gatgtattgc cctcagaggc ccggcacatc gaagaactgc gccgccgact gctcgacacc
      361 gcctgcactt acggctacga gctggtcatt ccgccgctga tcgagcacct ggattcgctg
      421 ctcaccggca ccggccaggc gctggaattg cagactttca agctggtcga tcagctctcc
      481 ggccgctcgc tcggcctgcg cgccgacacc acgccccagg tggcgcgcat cgatgcgcac
      541 ctgctggggc gccaggccat cacccgcctg tgctactgcg gtccggtgct gcacacgcgc
      601 cccgagcggc cgcacgccac gcgtgagccg ctgcagttcg gcgccgaaat ttacggccac
      661 gccgggcgcg aggccgatgt ggaaatcctc tcgctcgcgc tcgactgcct gcgcgccgcg
      721 ccgctggccg gcttgtcggt cgacctggcc gacgcccgca tcgtgcgcgc gctgctggcc
      781 ggcgtgatgg ccgccccgac ggtgctggcc gaggtgcacc acgcgctggc cgccaaggac
      841 gcgagcacgc tggggcggct gacgcgcgac tttcccgccg catcgcgcga agggctgcag
      901 gcgctggtcg gtttgtatgg tgatgaagag gtgctgattg aagccgaaaa agccctgcag
      961 cgcacgccgg gtgtgcgcga ggcgctatca aacttgaaat acctggcctc gcgcctggaa
     1021 ggcgtgcgtg tcagttttga cctggccgat gcacgcggct acggctacta caccggcgcg
     1081 cgctttgcgg tgtatgccga aggcgcgggc gatgccgtgc tgcgcggcgg ccgctacgac
     1141 ggcgtgggcg ccgtgtttgg ccacaagctc gatcgcgagc gcccggccgt cggcttcagc
     1201 ctggatgtga agcagctcgc gcgcgccgcc gccagcgcgc cgttgcgcgc ggccgtccgc
     1261 gcgccctgga gcgacgcgcc cggcttgcaa gcggccgtgc aggcgctgcg cgcgcgcggc
     1321 gaaaccgtgg tgtgcgtgct gcccggcagc cacagccaga tcgacgaatt caactgcgac
     1381 cgcgagctga tcgaagccgg cggccagtgg gtgatccaat ctctttgaag tgaaagacat
     1441 gaaaccaacc accggacgca acgtcgtcgt cgtcggcacc cagtggggcg atgaaggcaa
     1501 gggcaagctg gtcgactggc tgaccgaaag cgcgcaaggc gtggtgcgct ttcagggcgg
     1561 ccacaacgcg gggcacacgc tggtcatcaa cggcgtcaag acggcgctgc acctgatccc
     1621 gagcggcatc atgcgccctg gcgtgacctg ctacatcggc aacggcgtgg tgctctcggt
     1681 cagcaagctg ctggaagaaa tcgaaggcct ggaaaaagcc ggcgtgcagg tgcgcgagcg
     1741 cctgcgcatc agcgaaggct gcccgctgat cctgccgctg cacgccgcgc tcgacgtggc
     1801 gcgcgaagcc gcgcgcgaaa gcggcggcag cgagaagatc ggcacca
//
LOCUS       DAOMVO010000425         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18538_length_1841_cov_21.027996, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000425 DAOMVO010000000
VERSION     DAOMVO010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18538_length_1841_cov_21.027996"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKV30_13645"
     CDS             complement(<1..296)
                     /locus_tag="PKV30_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRL31296.1"
                     /translation="MPAAAPAAPAASSTARPGTTWDNPMGTDGFEFIEYAAPDPAAMG
                     ALFERMGFAAVAKHRHKNVTLYRQGEINFIINAEPDSFAQRFAKLHGPSICAIA"
     gene            481..960
                     /locus_tag="PKV30_13650"
     CDS             481..960
                     /locus_tag="PKV30_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL31297.1"
                     /translation="MNTLVLDRLDRAILTRLQADGRETHESIGAHIGLSASAVLRRVK
                     KLEEAGVIARYVALLDPPAIGLGLAAYVNVRLEKRAEHAKRNPMEEFGASVQTWPEVV
                     ECVSLTGEMDYLLHLVVQDMSAYSRFMMDTLLRHPSVQDCKTSFVMDQVKATTALPV"
     gene            1161..>1841
                     /locus_tag="PKV30_13655"
     CDS             1161..>1841
                     /locus_tag="PKV30_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL31298.1"
                     /translation="MDTRNPTPADSGTPAASSPARRSAIVPIREIGPRYRPQITQHLL
                     ALGEHDRYLRFGYAANDRQIRHYVDQLDFERDQIFGIFNRKLELIAMAHLAYPADMVQ
                     AGFAEFGVSVAHHARGRGYGSRLFERAAIHAVNDGVKTLYIHALSENTAMLRIARQAG
                     ATVERQGSESEAHLTLPAATFASHMGELFANQVGQVDYWLKTEAVRARDVLATVQEVR
                     EAVREGRHK"
BASE COUNT          307 a          590 c          610 g          334 t
ORIGIN      
        1 aaggcgatgg cgcagatgct ggggccgtgc agcttggcaa agcgctgggc aaagctgtcg
       61 ggctcggcgt tgatgatgaa gttgatctcg ccctggcggt acagcgtcac gttcttgtgg
      121 cggtgtttgg cgacggcggc aaagcccatg cgctcgaaca gcgcgcccat ggcggcgggg
      181 tcgggcgcgg cgtattcgat gaactcgaaa ccgtcggtgc ccatggggtt gtcccaggtg
      241 gtgccggggc gggcggtgga agaagcggct ggggcggctg gggcggcggc aggcatgtct
      301 tgtctccttg ggtgtcgggg cgaaacctca cactttaggt cggcggcgat cgcgtttttc
      361 agcgtcttga gcgcttcagc gggcaagcgg cgcacgtttc atgtgccata tgggctaggc
      421 gatgcgaatt tctttgacgt cgccagtcgt cgcgcctacg atgctgcgtt ttgcaccgcc
      481 atgaacaccc tcgtcctcga ccgccttgac cgtgccatcc tgacgcgcct gcaggccgat
      541 ggccgcgaga cgcatgaatc gattggcgcg cacatcggcc tgtcggccag cgccgtgttg
      601 cggcgcgtca aaaagctcga ggaagcgggc gtcatcgcgc gctacgtggc gctgctcgac
      661 ccgcccgcca tcggcctggg gctggccgcc tacgtcaacg tgcggctaga aaagcgcgcc
      721 gagcacgcca agcgcaaccc gatggaggaa ttcggcgcca gcgtgcagac ctggcccgag
      781 gtggtggagt gcgtttccct gaccggcgag atggattatt tgttgcacct ggtggttcag
      841 gacatgagtg cgtactcacg cttcatgatg gacacgctgc tgcgccaccc gtcggtgcag
      901 gactgcaaga ccagcttcgt gatggatcag gtgaaggcga ccacggcctt gcccgtttag
      961 ggatgttttt ggccaccagc gcttgtctgt ccagcgcaag aagctatcaa aatcgtagtt
     1021 ttgcggtctg cgctgaatct ttccgcgcca tggcccgcgc cggcccgaat gtgaattcct
     1081 ttgtaggaca ttgctgcaat gcagcatttt ttgctcgatt ggagttttct ctctacggtt
     1141 gagcccctac catgggctgc atggacactc gtaaccccac ccctgcggac agcggcaccc
     1201 ctgctgcatc gtcgccagca cgccgttcgg ccatcgtgcc gatccgagag atcgggccgc
     1261 gctaccgacc gcaaatcacg cagcacctgc tggcgctggg cgagcacgac cgctacctgc
     1321 gcttcggcta cgccgccaac gaccggcaga tccgccacta cgtcgaccag ctcgacttcg
     1381 agcgcgacca gatcttcggc atcttcaacc gcaagctgga actcatcgcc atggcgcacc
     1441 tggcctaccc ggccgacatg gtgcaggccg gctttgccga gttcggcgtg tcggtggccc
     1501 accatgcgcg cggccgcggc tatggctcgc gcctgttcga gcgcgccgcc atccacgccg
     1561 tgaacgacgg cgtgaagacg ctctacatcc acgcgctgag cgagaacacc gccatgctgc
     1621 gcatcgcccg ccaggccggc gccaccgtgg aacgccaggg cagcgagagc gaggcgcacc
     1681 tgacgctgcc cgcggccacc ttcgccagcc acatgggcga gctgttcgcc aaccaggtgg
     1741 gccaggtcga ctactggctg aagaccgaag ccgtgcgcgc ccgcgacgtg ctggccacgg
     1801 tgcaggaagt gcgcgaggcg gtgcgcgaag ggcgccacaa g
//
LOCUS       DAOMVO010000426         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18645_length_1833_cov_9.703037, whole genome shotgun sequence.
ACCESSION   DAOMVO010000426 DAOMVO010000000
VERSION     DAOMVO010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18645_length_1833_cov_9.703037"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKV30_13660"
     CDS             <1..311
                     /locus_tag="PKV30_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL31299.1"
                     /translation="REIVREHGVMLLLGSMRAEERLAAFLLNLVQRLHARGFSQSELV
                     LRMTREEIGSYLGLKLETVSRTFSKFADDGIVEVKQRYVRIVDTDALRAIVNAPACAD
                     "
     gene            complement(399..869)
                     /locus_tag="PKV30_13665"
     CDS             complement(399..869)
                     /locus_tag="PKV30_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRL31300.1"
                     /translation="MKVLNLHCAHGHGFEGWFASEDDFQDQLTRRLLECPMCGDSGVT
                     KLPSAPRLNLSGASAPAAASDRVRSSPETAPVAALPPAAMQAAWMHMVRQVMASTEDV
                     GERFADEARRIHYGEAEERGIRGQATSEQAEELLDEGIAVVALPVPPGFKSTLQ"
     gene            complement(880..1470)
                     /locus_tag="PKV30_13670"
     CDS             complement(880..1470)
                     /locus_tag="PKV30_13670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRL31301.1"
                     /translation="MRDDLREARVKGQEILKGGFLHAFRDEVRLPDGTLTQREYIVHP
                     GAVMIVPLLQGEGGAAQLVLERQYRYPVQQVVIEFPAGKLDAGESPLACAQRELREET
                     GYTASEWARAGVLHPVVSYSTEFIEVWFARGLTLGERRLDEGEFLDVFTASPEQLYAW
                     CRDSQVTDAKTLIGALWLQNVQRGDWALDWQRVGAA"
     gene            complement(1467..1775)
                     /locus_tag="PKV30_13675"
     CDS             complement(1467..1775)
                     /locus_tag="PKV30_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HRL31302.1"
                     /translation="MSQNLSVWLLILLAFVCANLPFVNQRLMTVVPLRAPVKNLAWRV
                     AELVVWYFIVIGIGMAMERALGQNQAQGWEFYAVTAALFLTLAFPGFVYRYLVHRGGR
                     "
BASE COUNT          319 a          629 c          584 g          301 t
ORIGIN      
        1 gccgcgagat cgtgcgcgag cacggcgtga tgctgctgct gggcagcatg cgcgcggaag
       61 agcggctggc cgcctttttg ctgaaccttg tgcagcgcct gcacgcacgc ggcttctcgc
      121 aaagcgagct ggtgctgcgc atgacgcgcg aggagattgg cagctacctg ggcctgaagc
      181 tggagacggt gagccgcacc ttctcgaaat tcgccgacga cggcatcgtc gaagtcaagc
      241 agcgctatgt gcggatcgtc gacaccgacg cgctgcgcgc gatcgtgaac gcgccggctt
      301 gcgctgactg aatctccctc tacggcgttg gcgcgtcgct ggttctcagt gtttttggcc
      361 gcgagcgctt gtccaacaag cgcaagcggc tatcaaaatc attgcaacgt gcttttgaag
      421 ccgggcggca cgggcagcgc aacgacggca atgccttcgt ccaacagctc ttcggcctgc
      481 tcgctcgtgg cctggccgcg gatgccgcgc tcctcggctt cgccatagtg gatacggcgc
      541 gcctcatcgg caaaacgctc gcccacgtcc tcggtgctgg ccatcacctg gcgcaccatg
      601 tgcatccagg ccgcttgcat ggccgctggc ggcaaggcgg cgacgggcgc ggtctcgggc
      661 gacgaccgta cgcggtcaga cgccgccgcc ggcgcgctgg cgccgctcag gttcaggcgc
      721 ggcgcgctgg gtaacttcgt tacgcccgag tccccgcaca tcgggcattc cagcaaccga
      781 cgcgtcagct gatcctgaaa atcgtcctcg gacgcaaacc agccctcgaa gccgtggccg
      841 tgcgcgcaat gcaggttgag caccttcatc ggttcgcttt caggcggcgc ccacacgttg
      901 ccagtcgagc gcccagtcgc cacgctgcac gttttgcaac cacagcgcgc cgatcaaggt
      961 cttggcgtcg gtgacttggc tgtcgcggca ccaggcatac agttgctcgg gcgaggcggt
     1021 gaacacgtcc aggaattcgc cctcgtccag ccggcgctcg cccagcgtca ggccgcgcgc
     1081 aaaccacact tcgatgaatt cggtcgaata cgagaccacc gggtgcagca cgccggcacg
     1141 cgcccattcg ctggcggtgt aaccggtttc ctcgcgcaac tcacgctgtg cgcaggccag
     1201 cgggctctcg ccggcgtcga gcttgccggc gggaaactcg atgaccacct gctgcaccgg
     1261 gtagcggtac tggcgctcga gcaccagttg cgccgccccg ccctcgcctt gcagcagcgg
     1321 cacgatcatc acggcgccgg gatgcacgat gtattcgcgc tgcgtcagcg tgccatcggg
     1381 caggcgcacc tcgtcgcgga aggcatgcag gaaaccgccc ttgagaattt cctgcccctt
     1441 cacgcgcgct tcgcgcaaat cgtctctcat cggccgcccc ggtgcacaag atagcggtag
     1501 acgaaacccg gaaacgccag ggtgaggaac agcgccgcgg tgacggcgta gaactcccag
     1561 ccctgcgcct ggttctggcc caacgcccgt tccatcgcca tgccgatgcc gatgacgatg
     1621 aagtaccaca cgaccagttc ggccacccgc cacgccaggt tcttgaccgg cgcgcgcagg
     1681 ggcaccacgg tcatcaagcg ctggttgacg aagggcagat tggcgcagac gaaggccagc
     1741 agaatcagca gccagaccga caggttctgc gacatcaggt gacgagcatc tgcacgatgg
     1801 ccttggcgca cagcgccatc agcgcgcccg gca
//
LOCUS       DAOMVO010000427         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18732_length_1827_cov_17.615688, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000427 DAOMVO010000000
VERSION     DAOMVO010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18732_length_1827_cov_17.615688"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..736
                     /locus_tag="PKV30_13680"
     CDS             263..736
                     /locus_tag="PKV30_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRL31303.1"
                     /translation="MNRLASILPASQVLVGVEATSKKRAFEEAGLLFENLHGLSRALI
                     TDSLFARERLGSTGLGHGVAIPHGRIKGLKAPMAAVLQLAQPIGFDAPDEQPVGLLIF
                     LLVPEAATQKHLEILSEIAELLSDADLRERIKSAASVDELHGLIANWKSAQQVTP"
     gene            918..>1827
                     /gene="hprK"
                     /locus_tag="PKV30_13685"
     CDS             918..>1827
                     /gene="hprK"
                     /locus_tag="PKV30_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRL31304.1"
                     /translation="MKPSVISADVLFEDHRATLRWHWVAGLQASERRFDEAAVSQARS
                     GADLVGYLNYIHPYRLQILGEREVAYLSNASPEDCARRIARIVTLEPPVLVLCDGQTA
                     PDALISMCERAQIPLFATQDSAAFVIDVLRAYLSKHFADRTTMHGVFMDILGLGVLIT
                     GESGLGKSELGLELISRGNGLVADDAVDLFRINQATIEGRCPELLQNLLEVRGIGLLD
                     IRAIFGESAVRRRMRLRLIVHLVRRETMERDYERMPYEPLTQDVLGVPIQKAVIQVVA
                     GRNIAVLVEAAVRNHILQLRGIDTYQE"
BASE COUNT          287 a          601 c          605 g          334 t
ORIGIN      
        1 caagcatttg atgtagtatc gtgtaacaac aacgagcgaa gactgtagac tccagcttac
       61 aagccgcgcc ggttcatgcc gggcggcttt ctttttctcg ccggacgatg tgtttcaaag
      121 cccgcagggg tatcgcgcca gggagcgcgt gccgctgtca ccgccattcg cgtgacttat
      181 tcggctgcgc ctgcccaccg cttggcgccg ccgggcacgg gtttgcccgg cctccagcgc
      241 gcataatcca gccgccatgt ccatgaaccg tcttgcctcc atccttccgg cgtcgcaggt
      301 gctggtcggc gttgaagcca ccagcaagaa gcgcgctttt gaagaagccg gtctgttgtt
      361 cgagaacctg cacgggctgt cgcgcgcgct gatcaccgac agcctgttcg cgcgcgagcg
      421 gctcggctcg accggccttg gccacggcgt ggcgattccg cacggccgca tcaagggctt
      481 gaaggcgccg atggccgcgg tgctgcagct ggcgcagccg atcggctttg acgcgccgga
      541 cgagcaaccc gtcggcctgc tgatctttct gctggtgccc gaggcggcga cgcaaaagca
      601 ccttgaaatc ctgtccgaga tcgccgagct gctgagcgac gccgacctgc gtgagcggat
      661 caagtcggct gcttcggtgg acgagctgca cggcttgatt gcgaactgga aatcggcgca
      721 gcaagttacc ccctgagccg ctgacgcggc ttcccctgga agggggacaa cgctggtggt
      781 cggggggacc ccgaccacgg cgttcccgaa tggcctgctc cgcggccttt tggggcttgc
      841 tgcgcagccc cgcgattcag ggctggtcgt gtgttgtcat cgcatagact ggcgcacgcc
      901 acttcttcgc gcccgccatg aaaccctccg tcatcagcgc cgacgtcctg ttcgaggacc
      961 accgggccac cttgcgctgg cactgggtgg cggggctgca ggcgtcggag cggcgctttg
     1021 acgaggcggc ggtcagccag gcgcgctcgg gtgctgatct ggtcggctac ctcaactaca
     1081 tccacccgta ccggctgcag attctgggcg agcgcgaggt ggcgtatctg tccaacgcct
     1141 cgcccgaaga ctgcgcgcgc cgcatcgcgc gcatcgtcac gctggagccg ccggtgctgg
     1201 tgctgtgcga cggccagacg gcgcccgacg cgctgatctc gatgtgcgag cgggcgcaga
     1261 ttccgctgtt cgccacgcag gattcggcgg ccttcgtcat cgacgtgctg cgcgcctacc
     1321 tgtccaaaca ttttgccgac cgcaccacca tgcacggcgt gttcatggac atcctgggcc
     1381 tgggcgtgct gatcaccggc gaatcgggcc tcggcaaaag cgagctgggg ctggagttga
     1441 tttcacgcgg caacggcctg gtggccgacg acgcggtgga tttgtttcgc atcaaccagg
     1501 ccaccatcga gggtcgctgt cccgagctgc tgcaaaacct gctcgaagtg cgcggcatcg
     1561 gcctgctcga catccgcgcc atctttggtg aatcggcggt gcgccggcgc atgcggctgc
     1621 gcctgatcgt gcacctggtg cgccgcgaga ccatggagcg cgactacgag cgcatgccct
     1681 acgagccgct cacgcaggac gtgctgggtg tgccgatcca gaaagccgtg atccaggtgg
     1741 tggccggccg caacatcgcc gtgctggtgg aagcggcggt gcgcaaccac atcctgcagt
     1801 tgcgcggcat cgacacctac caggaat
//
LOCUS       DAOMVO010000428         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18793_length_1822_cov_26.921336, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000428 DAOMVO010000000
VERSION     DAOMVO010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18793_length_1822_cov_26.921336"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKV30_13690"
     CDS             <1..627
                     /locus_tag="PKV30_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRL31305.1"
                     /translation="GLICGTWGTNQMHLTYIDQVIGKKPGARTYAAMNGLMLPGRQVF
                     LVDTHINVDPSAEQLADITIMAAQEMMRFGFKPKAALLSHSNFGSSNQPSAVKMRQAL
                     ALVQEQAPWLEVDGEMHGDVALDAGQRREVMPDSPLVGDATLLVMPNLDAANISYNLL
                     KVAAGGNIAIGPILLGADKPVHILTASTTVRRIVNMTALAVADANAPR"
     gene            785..1201
                     /locus_tag="PKV30_13695"
     CDS             785..1201
                     /locus_tag="PKV30_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRL31306.1"
                     /translation="MLRTAASHAVRLTRVVLAASVLAIALPTANVAVARESFWGATET
                     TIAVTELPRHGQQTYWLILQGGPFPHDKDGSVFGNRERLLPRERRGYYREYTVETPGA
                     RNRGARRIVCGGPQRTAPDACWYTSDHYSSFRKIVQ"
     gene            1282..1707
                     /locus_tag="PKV30_13700"
     CDS             1282..1707
                     /locus_tag="PKV30_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRL31307.1"
                     /translation="MDQPLRQPTESPLKGVRTNIVQSIRAFRVHDLQEAASALGQHFL
                     YANLANAQTKQDVLDMIAAQFTFPAHFGKNFDALYDCMTDPLHKSGPQPGFIVVLEQI
                     PATAKFDKEAREQLLDIFRDAADYWNDRKIPFRCFYSFR"
BASE COUNT          353 a          612 c          554 g          303 t
ORIGIN      
        1 ggcctgatct gcggcacctg gggcaccaac cagatgcacc tgacctacat cgaccaggtg
       61 atcggcaaga agcccggcgc gcgcacttac gccgccatga acggactgat gctgccgggc
      121 cggcaggtgt ttctggtcga cacgcacatc aacgtcgacc ccagcgccga gcaactggcc
      181 gacatcacca tcatggcggc gcaggagatg atgcgctttg gcttcaagcc caaggcggcg
      241 ctgctgtcgc actcgaactt cggctcgtcc aaccagccga gcgccgtcaa gatgcgccag
      301 gcgctggcgc tggtgcagga acaggcgccg tggctggaag tcgatggcga gatgcacggc
      361 gacgtcgcgc tggacgccgg tcagcggcgc gaggtgatgc ccgacagccc gctggtcggc
      421 gacgccacct tgctggtcat gcccaacctg gacgccgcca acatctccta caacctgctg
      481 aaagtggcgg ccggcggcaa catcgccatc ggcccgatcc tactgggcgc cgacaaaccg
      541 gtgcacattc tcacggccag caccaccgtg cggcgcatcg tgaacatgac ggcgctggcc
      601 gtggcggacg ccaacgcgcc gcgctagggc ctattcagac aagttagcgc ctactcagca
      661 ggcgctgaga ccttgcgcct ggtgcctgaa ttttaggcag gcgcttgctt ttttgtggcg
      721 cttgcgtcac actaccgcct tggaattttc gggtaaaccc gagggtttcc gaaaggtgta
      781 tttcatgttg cgaacagcgg catcgcatgc tgtccggttg actcgggttg ttctcgcggc
      841 ttcggtgctg gcaatcgcgc tccccacggc gaacgtggcc gtggcgcgag agtcgttctg
      901 gggcgctacc gagaccacca tcgcggtgac ggaattgccc cgacacggac agcagacgta
      961 ctggctgatc ctgcaaggcg gcccgtttcc ccacgacaag gacggctcgg tgttcggcaa
     1021 ccgcgaacgc ctgctgccgc gcgagcggcg cggctactac cgcgaataca ccgtcgaaac
     1081 ccccggggca cgcaaccgcg gcgcccgtcg catcgtctgc ggcggcccgc aacgcaccgc
     1141 gcccgatgcc tgctggtaca cgagcgatca ctactcgagc ttcagaaaga tcgtgcaatg
     1201 aaagcctttg aacagtttca gcccgccccg gcccagtcgc cgggcgaaga ccggtttttg
     1261 acttcagaga aagcagcgga gatggatcag ccacttcgtc aacccaccga atcgcccttg
     1321 aagggcgttc gcaccaacat cgtgcagtcg atccgcgcct ttcgcgtgca cgacctgcag
     1381 gaagccgctt cggcgttggg ccagcacttc ttgtacgcca acctggccaa cgcccagacc
     1441 aagcaggacg tgctggacat gattgccgcg cagttcacct ttccggcgca cttcggcaag
     1501 aacttcgacg cgctgtacga ctgcatgacc gacccgctgc acaagagcgg gccgcagccg
     1561 ggcttcatcg tcgtgctcga gcagatcccc gccacggcca agttcgacaa ggaagcgcgc
     1621 gagcagctgc tcgacatctt ccgcgacgcc gccgactact ggaacgaccg gaagataccg
     1681 tttcggtgct tctattcttt tcggtagccc gttctgcacc agccggccaa gcagaacggg
     1741 cgaatgaggc ccagggcgac aacatgccct ccgacactga aatcgccgac aacggcgaga
     1801 agatgccgac cgacaagctg gt
//
LOCUS       DAOMVO010000429         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18827_length_1820_cov_17.146742, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000429 DAOMVO010000000
VERSION     DAOMVO010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18827_length_1820_cov_17.146742"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKV30_13705"
     CDS             complement(<1..697)
                     /locus_tag="PKV30_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRL31308.1"
                     /translation="MTALGLDVARIRAITLDLDDTLWPIWPTIQRAEDALSAWLSTHA
                     PRTAALARNPDTRQRAREQVLADRPAQAHDLATIRREAIRQLLTWAGDAPLLAEPAFE
                     VFFAERQRVTLFTDALPALAALSACYPVVALSNGNADVHRVGIGQFFRGAVNATQIGV
                     GKPDRRIFTAAAQAAGVPASAVLHVGDDAHLDGAGALAAGMQVAWINRGGEHWPAAIP
                     GRPHAVAADMRALC"
     gene            747..>1820
                     /gene="hemH"
                     /locus_tag="PKV30_13710"
     CDS             747..>1820
                     /gene="hemH"
                     /locus_tag="PKV30_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRL31309.1"
                     /translation="MRFRPEPEMSHGQPARTGVLLCNLGTPDAPTAAATRRYLAEFLA
                     DPRVVEIPPAVWKPILHGVILRTRPAKSAAKYQQIWTPEGSPLMVWSRKQEVMLRGYL
                     GERGHDVSVRLAMRYGNPSIASQLDELKKRGCTRILVVPMYPQYSGTTTASVIDAVAD
                     WARTARHVPELRFVNRFHDDPGYIRALTRVVHQRWQSHGRPDKLVMSFHGVPERTLTL
                     GDPYHCECQKTARLLAERLALKDDEWVLTFQSRFGKAKWLEPATQTVLEQLGRAGTPH
                     VDVVCPGFVADCLETLEEIAMEGQSAFKAAGGKEFGYVPCLNDDPTWMAALAEIVQEH
                     LQGWPTQRRADDGEAAATRERARA"
BASE COUNT          287 a          593 c          676 g          264 t
ORIGIN      
        1 cgcacaaggc tcgcatgtcc gcggccaccg cgtgcgggcg cccggggatc gcagctggcc
       61 aatgctcacc gccacgattg atccacgcga cctgcatgcc ggcagccagc gcgccggcgc
      121 cgtcaaggtg ggcgtcgtcg ccgacgtgca gcacggcgct tgcgggcacg ccggcggctt
      181 gcgcggcggc ggtgaagatg cggcgatccg gcttgccgac gccgatctgc gtggcgttga
      241 cggcaccacg aaagaactgg ccgatgccta cgcggtgcac gtcggcattg ccgttgctga
      301 gcgcgaccac gggatagcac gcgctgaggg cggccagtgc gggcagggcg tcggtaaaca
      361 acgtcacgcg ctgccgttcg gcgaagaaga cctcgaaggc gggctcggcc agcaaagggg
      421 cgtcgcccgc ccaggtcagc agttggcgaa tggcttcgcg gcggatcgtg gccagatcgt
      481 gcgcttgcgc ggggcgatcg gccagcacct gctcacgcgc gcgctgccgt gtatcggggt
      541 tgcgcgccag tgcggcggtg cgcggggcgt gcgtgctcag ccaggcgctc aaggcatctt
      601 cggcgcgctg gatggtgggc cagatgggcc acagcgtgtc gtccagatcg agggtgatgg
      661 cgcggatgcg ggccacgtcg aggccgaggg cggtcatggc gggcggtggt tttcagggtc
      721 ggtcgaaggg ccggtcagaa taccgcatgc gttttcgacc cgaacccgag atgtcccacg
      781 gccagccagc gcgcaccggc gtgctgctgt gcaacctggg cacgcccgat gcgccgacgg
      841 cggccgccac gcggcgttac ctggctgaat tcctcgccga cccgcgcgtg gtggagattc
      901 cgccagcggt gtggaagccg atcctgcacg gcgtgatcct gcgcacgcgg ccggccaaat
      961 cggccgccaa gtaccagcag atctggacgc ccgaaggctc gccgctgatg gtgtggagcc
     1021 gcaagcagga ggtgatgctg cgcggctacc tgggcgagcg cgggcacgac gtcagcgtgc
     1081 ggctggccat gcgctatggc aacccgtcca tcgcctcgca actggacgag ctgaaaaagc
     1141 gcggctgcac ccgcatcctg gtggtgccga tgtacccgca gtactcgggc accaccaccg
     1201 ccagcgtgat cgatgccgtg gccgactggg cgcgcacggc gcggcatgtg cccgagctgc
     1261 gctttgtgaa ccgctttcac gacgaccccg gctacatccg cgcgctgacc cgcgtggtgc
     1321 accagcgctg gcaaagccat gggcgacccg acaagctggt gatgagcttt cacggcgtgc
     1381 ccgagcgcac gctgacgctg ggcgacccct accactgcga atgccagaag actgcccgcc
     1441 tgctggccga gcggctggcg ttgaaggacg atgaatgggt gctgacgttt cagtcgcgct
     1501 ttggcaaggc caagtggctg gagccggcca cgcaaacggt gctggagcag cttggccgcg
     1561 ccggcacacc gcacgtcgac gtggtgtgcc ccggcttcgt cgccgattgc ctggagacgc
     1621 tggaagaaat cgccatggag gggcagtcgg cgttcaaggc cgcgggtggc aaggagtttg
     1681 gctacgttcc ttgcctgaac gacgacccga cgtggatggc ggcgctggcc gagatcgtgc
     1741 aggagcatct gcaaggctgg ccgacgcagc gccgcgcgga cgacggcgaa gcggctgcca
     1801 cgcgcgaacg cgcgcgggcg
//
LOCUS       DAOMVO010000430         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18828_length_1820_cov_16.573371, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000430 DAOMVO010000000
VERSION     DAOMVO010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18828_length_1820_cov_16.573371"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKV30_13715"
     CDS             complement(<1..1442)
                     /locus_tag="PKV30_13715"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRL31310.1"
                     /translation="MTTPAYPHTQLHIDGQWCDAEGGKTMAVFNPATGAEIGRLAHAS
                     RADLDRALAAAHKGFETWRAVPAHERAALMRKAAQIFRERADETARHLTLEQGKPLPE
                     AKGEVLAACDVIEWMAAEGMRVYGRIVPPRDLRGTAHVMKQPVGPVAAFTPWNFPVNQ
                     VVRKLSAALATGCSIIVKAPEETPASPAALIRAFVDAGVPAGVVQLVYGVPAEISQYL
                     IPHPVIRKITFTGSTPVGKQLAALAGQHMKRATMELGGHAPVIVCEDADIDLAIKAAG
                     GAKFRNAGQVCISPTRFLVARSIAAEFTKKFAAQAEKTTVGDGMVEGTRMGPLANPRR
                     VAAMQALTDDAVKRGGTVAAGGEKIGDAGNFWAPTVLADVPLDADVFNTEPFGPMAVV
                     HAFDSLDDALREANRLPFGLAGYAFTQSLKNAHQISQQLEVGLLWINQPAAAWPELPF
                     GGIKDSGYGSEGGPEALEAYLTTKSVSTFCG"
BASE COUNT          267 a          588 c          658 g          307 t
ORIGIN      
        1 ccgcaaaacg tcgacaccga cttggtcgtc aaatacgcct ccagcgcctc tggcccgcct
       61 tccgagccat agcccgaatc cttgatgccg ccaaagggca gttcgggcca ggcggcggcg
      121 ggctggttga tccacagcag gccgacttcg agttgctggc tgatctggtg cgcgtttttc
      181 aacgactgcg tgaaggcgta gccggccagg ccgaagggca ggcggttggc ttcgcgcagg
      241 gcgtcatcca ggctgtcgaa ggcgtgcacc accgccatgg ggccgaaagg ctcggtgttg
      301 aacacgtcgg catccagcgg cacgtcggcc agcacggtgg gcgcccaaaa gttgccggcg
      361 tcgccgattt tctcgccgcc cgcggccacg gtgccgccgc gcttgaccgc atcgtcggtc
      421 agtgcctgca tggcggccac gcggcgcggg ttggccagcg ggcccatgcg cgtgccttcg
      481 accatgccgt cacccaccgt ggttttctcg gcctgcgcgg cgaatttctt ggtgaactcg
      541 gcggcaatcg acctggcgac cagaaagcgc gtcggcgaga tgcacacctg acccgcgttg
      601 cggaacttgg cgccgccggc ggccttgatg gcgagatcaa tgtcggcgtc ttcacacacg
      661 atcaccggcg cgtggccgcc cagttccatc gtcgcgcgct tcatgtgctg gccggccagc
      721 gccgccagtt gcttgcccac cggcgtggag ccggtgaacg tgatcttgcg gatgacgggg
      781 tgcgggatca ggtattgcga aatctccgcc ggcacgccgt agacgagttg caccacgccg
      841 gccggcacgc cggcatcgac aaaagcgcgg atcagcgcgg cgggcgaggc gggggtttct
      901 tccggcgcct tgacgatgat cgagcagccc gtggccagcg ccgccgacag cttgcgcacc
      961 acctggttga cggggaagtt ccatggcgtg aaggccgcca ccgggcccac cggctgcttc
     1021 atgacgtgcg ctgtgccgcg cagatcgcgc ggcggcacga tgcggccgta cacgcgcatg
     1081 ccttcggcgg ccatccactc gatgacgtcg caggcggcca gcacttcgcc cttagcctca
     1141 ggcaaaggct tgccttgctc cagggtcagg tggcgcgcgg tctcgtcggc gcgctcgcgg
     1201 aagatttgcg ccgccttgcg catcagcgcg gcgcgttcgt gcgcgggcac ggcgcgccag
     1261 gtctcgaagc ctttatgcgc agcggcaagc gcgcggtcaa ggtcggcgcg gctggcgtgg
     1321 gccaggcggc cgatctcggc gccggtggcg gggttgaaca cggccatggt cttgccgcct
     1381 tcggcgtcgc accattggcc gtcgatgtgg agttgggtgt gggggtaggc gggggtggtc
     1441 atgatggggc tcctgaatgg gtgaatggcc gaacgagggc gcgcgggcaa tcgtacacgt
     1501 gacgtggggt gcggctgggt tcaggggctt gtgttgggct cggggatggg atggaggcgc
     1561 tgtcggccgg gagttcgccc cggcgggcga cctacttttc ttgctcgcac aagaaaagta
     1621 ggcaaaagaa gtgcgcccca ctggccgtgg ccctgcgggc agcctgcgat gctcgtgcgc
     1681 ggggcggcgc tgccgaactc acttcgcgct tacagcgctc cgttcaaaca gcggcagcga
     1741 gtcagagcac gaagcgcgca tgcttcgctg cgcgcccgcc ccacgccctg cgcttctcgg
     1801 cacggccaga agggggttga
//
LOCUS       DAOMVO010000431         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18871_length_1817_cov_11.203178, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000431 DAOMVO010000000
VERSION     DAOMVO010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18871_length_1817_cov_11.203178"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKV30_13720"
     CDS             <1..322
                     /locus_tag="PKV30_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31311.1"
                     /translation="LGLHAVHQDRADHAAPADQADGVSDAFHLLCAPFNQSSECLTGP
                     AGSSWLREAGARRTRLGQPFLVVVVMEEAMNSPEPAQRRAVGSKRSAAFIVGQIVMER
                     PTPA"
     gene            complement(490..945)
                     /locus_tag="PKV30_13725"
     CDS             complement(490..945)
                     /locus_tag="PKV30_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRL31312.1"
                     /translation="MTTQRHPATAPVPANKTTDAASASRQIDALIARLDDWRGAQLSE
                     VRALIRAADPQVEKQWKWMGTPAFAHDGIYAIANPHKGKAKITFGHGAQLPDPHGLFN
                     AGLGGKKCRAIDLLEGDRLNKTAFKQLVRAAVKETVARAKAKEGTKGKA"
     gene            complement(959..1690)
                     /locus_tag="PKV30_13730"
     CDS             complement(959..1690)
                     /locus_tag="PKV30_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRL31313.1"
                     /translation="MPQITPKILAFDVFGTVVDWHGSIAAEVRQLYPQVDGDAFALAW
                     REGYQPAMQRVRSGELGWTKIDDLHRLILDSILPRFGMGDLPENERRTLNRVWHRLNP
                     WPDVVEGLSRLKRRHIITTLSNGNIGLLTDMAKHGELPWDCVLSAEVFRAYKPDPRTY
                     LGVADVFDVAPSEVMMVAAHQEDLAAARDCGLLTAYIERPLEFGAARPKDVSPAAENT
                     LHARDFIDLARQLALDAVPADDDLV"
BASE COUNT          304 a          600 c          576 g          337 t
ORIGIN      
        1 cctcggcctg catgcggtcc atcaggaccg agccgaccat gccgcgccag ccgaccaggc
       61 cgacggtgtt tccgatgctt ttcatttgct ttgcgcgcct ttcaatcaat caagtgaatg
      121 cttgactggc cccgccggct cgtcatggct gcgggaggcg ggcgcacgac gaaccaggct
      181 gggtcagccc tttttggtgg tcgtggtgat ggaggaggca atgaacagcc ccgaacctgc
      241 acagcgtagg gcggtcggat cgaagcgatc agcggcgttc atcgtggggc agattgtaat
      301 ggagcggccc acgcccgcct gacgctgaac gccggccgtc agcgcagctg ttggccgtgc
      361 gcgacgcggc tggcgccgtg gcaccgatgg tttggataag aaaagtggcc ctggcgcatg
      421 tcagacaagc gcgaacagct atcaaaaaca aagtaacggg ctctgcgctc cgttggccac
      481 gcacccaggc tacgccttgc ccttggtgcc ttccttcgcc ttcgcgcgcg ccacggtctc
      541 cttcaccgcc gcgcgcacca gttgcttgaa cgccgtcttg ttcaagcggt cgccctccaa
      601 caaatcaatc gcccggcact tcttgccgcc caggcccgcg ttgaacagcc cgtgcggatc
      661 aggcaactgt gcgccgtgcc caaacgtgat cttcgccttg cccttgtgcg ggttggcgat
      721 ggcgtaaatg ccgtcgtgcg caaaggcggg cgtgcccatc cacttccatt gcttctccac
      781 ctgcgggtcg gcggcgcgga tcagcgcgcg aacttcggac agttgcgcgc cgcgccagtc
      841 gtccaggcgg gcgatcaagg cgtcgatctg ccggctggcg ctggccgcgt cggtggtctt
      901 gttggctggc acgggcgccg tggccgggtg gcgctgggtg gtcatcggtc aacctcgctc
      961 aaacaaggtc gtcgtccgca ggcactgcat ccagtgccag ttgccgcgcc aggtcgatga
     1021 aatcgcgcgc gtgcagcgtg ttttcggccg ccggcgaaac atcctttggc cgcgccgcgc
     1081 caaactccag cggccgctcg atatatgccg tcagcagccc gcaatcgcgc gcggcagcta
     1141 aatcttcctg gtgcgcggcc accatcatca cttcggaagg cgccacgtcg aacacatcgg
     1201 caacgcccag gtaggttcgt ggatccggct tgtaggcgcg aaacacctcg gccgacagca
     1261 cgcaatccca cggcagctcg ccgtgcttgg ccatgtcggt cagcaggccg atgttgccgt
     1321 tcgacagcgt ggtgatgatg tgtcggcgct tgagccgcga cagcccctcc accacgtcgg
     1381 gccacgggtt gagccggtgc cacacgcggt tgagcgtgcg gcgctcgttc tcgggcaggt
     1441 cacccatgcc aaagcgcggc aggatgctgt ccaggatcag ccgatgcaga tcgtcaatct
     1501 tcgtccaccc cagttcgccc gaccgcaccc gctgcatcgc cggctgatac ccctcgcgcc
     1561 acgccagcgc aaaggcgtcg ccatccacct gcggatatag ctgccgcacc tccgcagcga
     1621 tggagccgtg ccagtcgacc acagtgccga agacgtcgaa ggcgaggatt tttggggtga
     1681 tttggggcat gggtcggcgt cctgtctgcg cgggcagcta tgaatttgag agtctctcag
     1741 tggccttgtt cactgcacgg agagtacgcc acaatgtgcg cgacttgcac cccgaaacac
     1801 tgaattggag ccatgtg
//
LOCUS       DAOMVO010000432         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_18988_length_1809_cov_11.267389, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000432 DAOMVO010000000
VERSION     DAOMVO010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18988_length_1809_cov_11.267389"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKV30_13735"
     CDS             complement(<1..524)
                     /locus_tag="PKV30_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRL31314.1"
                     /translation="MPLSPPAARSHLHDRRAVFRGYRRGDGLWDIEAELHDSKTQDFV
                     LPGEGRWAAGEPIHGMAIRLTVDNDTVVHAIEVATTPPCPTATTRPCTPPTGARSTAS
                     LRLSACSALNPCAATTPPAWCSRRWACGRVGQPSLGGGRAPVDQLDRRQRRSARFDAP
                     DDFRIDRDPALNLL"
     gene            754..1224
                     /locus_tag="PKV30_13740"
                     /pseudo
     CDS             754..1224
                     /locus_tag="PKV30_13740"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
     gene            1289..>1809
                     /locus_tag="PKV30_13745"
     CDS             1289..>1809
                     /locus_tag="PKV30_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRL31315.1"
                     /translation="MPATPALVDLYPGTLTVVRGLRVGQFTDPRRPTGCSVVIAPEGA
                     VGGVDVRGAAPGTRETDLLGPANLVGVVHAVLLSGGSAWGLDAASGVMRWLEEHGIGL
                     RVGAAPGQTVPIVPAAVLFDLHVGDARIRPGADAGYAACAAASRDAPTQGNVGAGAGA
                     TVGKLFGMGHAMKG"
BASE COUNT          239 a          620 c          674 g          276 t
ORIGIN      
        1 tagagcaggt tcagcgccgg gtcgcggtcg atgcggaagt catcgggcgc gtcaaagcgc
       61 gcggatcgcc gttggcggcg atccagttga tcgacaggcg ctcgcccgcc gccaaggctt
      121 ggctgcccaa ctcgaccgca ggcccagcgc ctgctgcacc atgctggcgg cgttgtcgcg
      181 gcacacgggt tcaaagcgct gcatgcgctc aggcgcaaag aagccgtcga gcgcgcgcct
      241 gtaggcggtg tgcaggggcg ggtcgtagcc gttgggcacg gcggggtggt cgccacctcg
      301 atggcgtgca ccacggtgtc gttgtccacc gtcaggcgga tcgccatgcc gtggatcggc
      361 tcgccggcgg cccagcggcc ttcgccgggc agcacgaaat cttgcgtctt gctgtcgtgc
      421 agttcggcct cgatgtccca caggccatcg ccccggcggt agccacgaaa caccgcccgg
      481 cggtcgtgca ggtgagagcg ggcagcgggc ggcgaaaggg gcacggcgaa aacctgtcgg
      541 acaatggcgt tgaccccgat tctggccgca agcgggccgc tgcgcagcgg aaccaccggc
      601 cgggccagcg agtcagcaca caccatgtcc ttgggcatca ggcttcggcg cctcatcccc
      661 acgcgtgaac agctgcaggc gggccgcttg acgcgctggc tggcgccgtt tctgggccac
      721 gccaagctgt ggcactgatc gcgccggggc gtcgctgccg gcgtggcggt gggcatcttc
      781 ttcggcttgc tggtgccgat tgcgcagatt ccgctgtcgg tcggcgcggc catcgtcatg
      841 cgcgccaacg tgccggcagc cgtcagcagc acgctggtca gcaacccgtt gatctttgcg
      901 ccgatctact acctggccta ccgcacgggc ttgcttgtca ccggcgacga ggtgcgccac
      961 cccgagcgag aactggcgcg catcaccgcg cgcgccgacg ccgacgaggc gcgtgccggc
     1021 ggggtgcggg gcgtggtcga gcgcgtgcgg gcgctgggca agccgctgat catcgggctg
     1081 gcgatctttg ccgtggccgg gggactgctg gcctatgtac tgatctgggc cggctggtgg
     1141 ctgtgggtgc accgcaagcg gcggcgcaag gtggcccgct acgcgcgggc acgctcggct
     1201 gcggacacgc cggccgaccg ctgagcgcgc tcacccgcgc ggtcacaatg ggcgccagcg
     1261 ccgccttgtc gtcacttgat cacgcaccat gcccgccacc cctgctcttg ttgacctcta
     1321 ccccggcacg ctgaccgtcg tgcgcggcct gcgcgtgggc cagttcaccg acccgcgacg
     1381 gcccaccggt tgcagcgtgg tcatcgcacc cgaaggcgcc gtgggcggcg tcgatgtgcg
     1441 cggcgcggca ccgggcacgc gcgagaccga tttgctgggc cccgccaacc tggtcggcgt
     1501 ggtgcatgcc gtgctgctgt cgggcggcag cgcctggggg ctggacgctg ccagcggcgt
     1561 gatgcgctgg ctggaagagc atggcatcgg cctgcgcgtg ggcgcggcac cggggcagac
     1621 cgtgcccatc gtgcccgccg ccgtgctgtt cgatctgcac gtgggcgatg cgcgcatccg
     1681 gcccggcgcc gacgcaggct atgcggcctg cgcagccgca agccgcgacg cgcccacgca
     1741 gggtaatgtc ggcgcaggtg ccggcgccac ggtgggcaag ctgttcggca tggggcatgc
     1801 gatgaaggg
//
LOCUS       DAOMVO010000433         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_19109_length_1801_cov_13.711913, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000433 DAOMVO010000000
VERSION     DAOMVO010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19109_length_1801_cov_13.711913"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKV30_13750"
     CDS             complement(<1..560)
                     /locus_tag="PKV30_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31316.1"
                     /translation="MRHTYILIGLNPSERSLLESLVALDPREEEQLVPVRRQEDAHLI
                     IANGDDRTVVETLRANNPQALIVLVGQPPGHAVTDLPVLRRPLEMNAVIDVLSQLDWP
                     SHLHSSEPTDFGFTFSPSTNHPPTPSPSTRSPPSEHATSAAPQDSSAFAPTTASMVMT
                     AGATATHSDVAPVAAPAPAPAPAVSAR"
     gene            complement(809..>1801)
                     /gene="glcF"
                     /locus_tag="PKV30_13755"
     CDS             complement(809..>1801)
                     /gene="glcF"
                     /locus_tag="PKV30_13755"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRL31317.1"
                     /translation="VQYGHLVDIGRQIVDAKVPRPPAEALTRRLLRCGLTSPLFGPAM
                     RLGQLVRGFLPAALKAKVPERRAAGAWPRRAHARKVLLLEGCVQPAMAPGINAATARV
                     LDAAGIECVLARGQGCCGAVKFHLADSAGGLAQMRANIDAWWPHIERGVEHGGVEAIV
                     INASGCGAMVKDYGHLLRHEPRYADRAEKVSALARDLSELLPDLVGALQSRVRPPADT
                     LAWHAPCTLQHGQKLRGGVEHYLGVLGFTLRPAAQEAHLCCGSAGTYSVLQPALSTQL
                     RERKLGHLLGESASERPAAVLSANIGCITHLQAGTEVPVRHWVELLDEALIASK"
BASE COUNT          263 a          630 c          638 g          270 t
ORIGIN      
        1 cgcgccgaca cggccggcgc cggcgccggc gctggcgcgg cgacgggcgc gacgtcgctg
       61 tgcgtcgccg tggcgccagc cgtcatcacc atcgacgccg tggtgggcgc gaaggccgag
      121 gaatcctgcg gcgccgccga cgtggcgtgc tctgacggcg gcgatcgggt cgacggcgac
      181 ggcgtcggcg ggtggttggt cgacgggctg aaggtgaagc cgaagtcggt cggctcgctg
      241 ctgtgcaggt ggctcggcca gtcgagctgg ctcagcacgt cgatcaccgc gttcatctcc
      301 agcggacggc gcagcaccgg cagatcggtc accgcgtggc ccggcggctg gccgaccagc
      361 acgatcagcg cctgcgggtt gttggcgcgc agggtctcga ccaccgtccg gtcgtcgcca
      421 ttggcaatga tcaggtgcgc atcttcctgc cggcgcactg gcaccagttg ctcttcctcc
      481 cgcgggtcga gcgcgaccag cgactcgagc agcgagcgct cggaaggatt gagaccgatg
      541 aggatgtagg tgtggcgcaa aacagtctgg gtctgaaggg gctggcgcgc tgggaaaggc
      601 gcgccttgaa ttggcttgat tttggccgct gcggccggcc ctgtcgaccg cgacgccgcc
      661 tgtctgccga cacaggcgcg gcaagggccg attgctgcac cgcagcgttg ccctcaggcc
      721 gcgcaagtgc gacttttgca cggtggatga agcggattta tcagtgtttc aaggcctcag
      781 cgaacgtcca gcaagcgcag acagctatct attttgaagc aatcagtgct tcgtccagca
      841 gttccaccca gtgccgcacc ggcacctcgg tgccggcctg aagatgcgtg atgcagccga
      901 tgttggccga cagcacggcg gccgggcgct cgctggccga ttcgcccagc aggtggccga
      961 gcttgcgctc gcgcagctgc gtcgacagcg cgggttgcaa caccgaatac gtgcccgccg
     1021 agccgcagca caggtgcgcc tcttgcgccg ccggccgcag cgtgaaaccg agcacgccca
     1081 ggtaatgctc caccccgccg cgcagctttt gcccatgctg cagcgtgcag ggcgcgtgcc
     1141 aggccagcgt gtcggccggc ggccgcacgc ggctttgcag cgcgcccacc agatcgggca
     1201 gcagctcgct caaatcgcgc gccagcgccg acactttttc ggcccgatcg gcgtaacgcg
     1261 gctcgtggcg cagcaggtgg ccgtagtcct tgaccatggc gccgcagccc gaggcgttga
     1321 tgacgatggc ttccaccccg ccatgttcca cgccgcgctc gatgtgcggc caccacgcat
     1381 cgatgttggc gcgcatctgc gccaggccgc cggcgctgtc ggccaggtgg aacttcaccg
     1441 cgccgcaaca gccctgaccg cgcgccagca cgcattcgat gccggccgca tccagcacgc
     1501 gcgcggtggc tgcgttgatg cccggcgcca tggccggttg cacgcagcct tccagcagca
     1561 gcaccttgcg cgcgtgcgcg cggcgcggcc aggcgccggc ggcgcggcgc tccggcacct
     1621 tggctttcag cgccgccggc agaaagccgc gcaccaactg gcccaggcgc atcgcggggc
     1681 cgaacagcgg cgaggtcagc ccgcaccgca gcagccggcg cgtgagcgct tcggccggcg
     1741 ggcgcggcac cttggcgtcg acgatctggc ggccgatgtc gaccaggtgg ccatactgca
     1801 c
//
LOCUS       DAOMVO010000434         1801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_19110_length_1801_cov_13.121993, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000434 DAOMVO010000000
VERSION     DAOMVO010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19110_length_1801_cov_13.121993"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1143
                     /locus_tag="PKV30_13760"
     CDS             361..1143
                     /locus_tag="PKV30_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL31318.1"
                     /translation="MTPSNDSIRFGLAGRVAIVTGASQGIGEACARRFAQEGAQVVIA
                     DVADARGQALADELKATYVHCDVGDKAQVDALVASVLQAHGRIDVLVNNAGIFKAADF
                     LDVTEADFDAVLRVNLKGSFLVAQAVAREMAKAGRGSIVNMSSVNSVLAIPNIASYNV
                     SKGGINQLTRAMALALADKGIRVNAVGPGTIATELAAAAVLTSDEAKNKIMMRTPLKR
                     LGEPSEIADVVAFLASDASSYITGEIIMVDGGRMALNYTVPV"
     gene            1140..>1801
                     /locus_tag="PKV30_13765"
     CDS             1140..>1801
                     /locus_tag="PKV30_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRL31319.1"
                     /translation="MNRARIIGSAPPSFDPPDLTTMRVIVAVAESGSMSAGSDRAGLA
                     LGAVSGRITALERATGTALFERSNRGVRTTATGQFLVQRARELLAEADRLTLDLHDAA
                     RQLQGHVRVMANASSIIEYLPARLAAFQAAHPRIRVEVEERSSPEIPLALLDGSADLG
                     LLDMPYAVQGLDAVDLFSDTLVVVVPAGHALARQRVLDMDALMELPLVCLPDGNAISG
                     RLT"
BASE COUNT          295 a          638 c          592 g          276 t
ORIGIN      
        1 cggggcgacg tcggcatggc gcgcctgctg ctgggcgccg gcgccgaccc gcgccgtgcc
       61 gaccgcgacg gcgccagcgc catcgactac gcccgccgcg ccggccacga ggcgctggcg
      121 cggcatcttg aagaggcaaa gtgaccccct gtgccgcctt cggcgtcttc ccccaagggg
      181 gacaacgccc gtggccgggc caagcccgtt ccacggcgtt cgcgcgcggc ttggtgcgcg
      241 gccatgcgcg cgcacgactg ccgtgttttc aggccactat gaatcccata gctgctcacg
      301 ctttcccaga aagcgccagc gccctttttc atcctcaacc tcactgaacg aaaagcaccc
      361 gtgacccctt ccaacgattc catccgcttc ggccttgcag gccgtgtcgc catcgtcacc
      421 ggcgccagcc agggcattgg cgaagcctgc gcgcgccgct ttgcgcaaga aggcgcgcag
      481 gtcgtcatcg ccgacgtggc tgatgcgcgc ggccaggcgc tggccgatga gctgaaggcc
      541 acctacgtcc attgcgacgt gggcgacaag gcccaggtcg atgcgctggt ggcctccgtc
      601 ttgcaagcgc acggccgcat cgacgtgctg gtcaacaacg ccggcatctt caaggcggcc
      661 gatttcctcg acgtcaccga ggccgacttc gacgccgtgc tgcgcgtcaa cctcaagggt
      721 tcgttcctgg tcgcgcaggc cgtggcgcgc gaaatggcca aggcggggcg aggcagcatc
      781 gtcaacatga gcagcgtcaa cagcgtgctg gcgattccga acatcgccag ctacaacgtc
      841 agcaagggcg gcatcaacca gctgacgcgc gcgatggcgc tggcgctggc cgacaagggc
      901 attcgcgtca acgccgtcgg ccccggaacc atcgccaccg aactggccgc cgccgccgtg
      961 ctgaccagcg acgaggccaa aaacaagatc atgatgcgca cgccgctcaa gcgcctgggc
     1021 gagccgagcg agattgccga cgtagtggct ttcctcgcca gcgatgcgtc cagctacatc
     1081 acgggcgaaa tcatcatggt cgacggcggg cgcatggcgc tgaactacac ggtgccggtg
     1141 tgaatcgtgc gcgcatcata ggcagcgcac ctccatcctt cgacccgccc gacctgacca
     1201 cgatgcgcgt catcgtggcg gtggctgaaa gcggctccat gagcgccggc agcgaccggg
     1261 cgggattggc gctgggcgcg gtgagtgggc gcatcaccgc gctcgaacgc gccaccggca
     1321 cggcgttgtt cgaacgctcc aaccggggcg tgcgcaccac cgccactggc caatttctgg
     1381 tgcaacgtgc gcgtgaactg ctggccgagg cggaccgcct gacgctcgac ctgcacgacg
     1441 ccgcacggca gttgcaaggc catgtacggg tcatggcgaa cgcctcgtcc atcatcgagt
     1501 acttgcctgc tcggctggcg gcgttccagg ccgcgcaccc gcgcatccgc gtcgaggtcg
     1561 aagagcgcag cagccccgag attccgctgg ccctgctcga tggcagcgcc gatctgggcc
     1621 tgctcgacat gccctacgcc gtgcagggcc tcgacgcggt cgacctgttc agcgacacgc
     1681 tggtggtggt ggtgccggcc ggtcacgcgc tggcacgcca gcgcgtgctg gacatggacg
     1741 cgctaatgga gctgccgctt gtgtgcctgc ccgatggcaa cgccatctcc ggccggctga
     1801 c
//
LOCUS       DAOMVO010000435         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_19858_length_1750_cov_16.925664, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000435 DAOMVO010000000
VERSION     DAOMVO010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19858_length_1750_cov_16.925664"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKV30_13770"
     CDS             complement(<1..1171)
                     /locus_tag="PKV30_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HRL31320.1"
                     /translation="MFKIKTYNAISVKGLERFARDKFEVGSSIEQPDAILLRSHKLQG
                     EPVPDSLLAVARAGAGVNNIPVDDYGKQGIVVFNTPGANANAVKELVLAGMLLATRGI
                     LPGIAYAQSLGGMTDSAAMHALLEKEKARFAGGELRGKTLGIVGLGAIGSMVADMALA
                     LGMKVLGFDPALSIDAAWRLSNAVQRMENLPALLGRSDYVSLHVPALDATRHMINAQV
                     LAGIKPGAVLLNFARESIVDPAAVVNALHSGKLGRYVCDFPEPGFAQEPKIIALPHIG
                     ASTEESEENCAVMGADQLIDFLENGHISNSVNYPAIRMDRTPGATRITIANDNVAGVL
                     GHVLGVLADAQVNVLDMSNRSRDALAYNIIDVESAPDGKVIETIRDVGNVIRVRVV"
     gene            complement(1349..>1750)
                     /locus_tag="PKV30_13775"
     CDS             complement(1349..>1750)
                     /locus_tag="PKV30_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine aminotransferase"
                     /protein_id="HRL31321.1"
                     /translation="GDTINTPSMLCVADYLDALKWCESVGGVQGTIARSQANLKVIED
                     FVADNPWISFLAKTKETRSNTSVCLSLDLPEDKVKALVKLLETEGVAYDIGSYKDAPP
                     GIRIWCGATVEKSDLEALMPWLNWAYEQVKG"
BASE COUNT          271 a          566 c          571 g          342 t
ORIGIN      
        1 acaccacccg cacccgaatc acattcccca cgtcgcgaat cgtttcgatc actttgccat
       61 ccggcgccga ctccacatca atgatgttgt acgccagcgc atcccggctg cggttgctca
      121 tgtcgagcac gttgacttgc gcgtcggcga gcacgccgag cacgtggccc agcacgcccg
      181 ccacgttgtc gttggcgatg gtgatgcgcg tggcgccggg ggtgcggtcc atgcggatgg
      241 cggggtagtt gacgctgttg ctgatgtggc cgttttccag aaagtcgatc agctggtcgg
      301 cgcccatcac ggcgcagttt tcttccgatt cctcggtgct ggctccgatg tgcggcaggg
      361 cgatgatctt tggctcttgc gcaaagcccg gctcgggaaa gtcgcacacg tagcgcccga
      421 gctttcctga atgcagcgcg ttcaccacgg ccgccgggtc gacgatggac tcgcgcgcaa
      481 agttgagcag cacggcgccg ggcttgatgc cggccagcac ctgcgcgttg atcatgtggc
      541 gcgtggcatc gagcgccggc acgtgcaggc tgacatagtc ggagcggccg agcagcgcgg
      601 gcaggttctc catgcgctgc accgcgttgg acaggcgcca ggcggcgtcg atcgacagcg
      661 ccgggtcgaa gcccagcacc ttcatgccca gcgccagcgc catgtcggcc accatggagc
      721 cgatggcgcc gagacccacg atgcccagcg tcttgccgcg cagttcgcca ccggcgaacc
      781 gcgctttttc tttttcaagc agcgcgtgca tggcggccga atcggtcatg ccgcccagcg
      841 actgcgcata cgcgatgccg ggcaggatgc cgcgcgtggc cagcagcatg ccggccaaca
      901 ccagttcctt caccgcgttg gcgttggcgc cgggcgtgtt gaagacgacg atgccctgct
      961 tgccgtagtc atcgacgggg atgttgttga cgcccgcgcc ggcgcgcgcc acggcgagca
     1021 gcgaatcggg caccggctcg ccctgcagct tgtggctgcg cagcaggatg gcgtcgggct
     1081 gctcgatgct gctgccgacc tcgaacttgt cgcgcgcaaa gcgctccagc cccttgaccg
     1141 aaatggcgtt gtaggtcttg atcttgaaca tgcgctgtct cctgttttta tagcggcttg
     1201 cgcttgtcag gcaagcgctg gagggcgatt ttgcttgtat tcgctcgccc ctttggggag
     1261 agggcagtgt gaggggcggc ggcgctcgcg gtgggcgtgg gctaccctca ccccagccct
     1321 ctctcagagg gagagggagc aagtcaggtc agcccttgac ctgctcgtac gcccagttca
     1381 gccaaggcat cagcgcctca agatcgcttt tttccaccgt ggcgccgcac cagatgcgga
     1441 tgccgggcgg cgcgtccttg tacgagccga tgtcgtaggc cacgccttcg gtctccagca
     1501 gcttgaccag cgccttgacc ttgtcttcag gcagatcaag cgacaggcac acgctggtgt
     1561 tcgagcgcgt ctcttttgtc ttggcgagga acgagatcca cgggttgtca gcgacgaaat
     1621 cctcgatcac cttcaggttg gcctggctgc gcgcgatggt gccttggacg ccgcccacgc
     1681 tctcgcacca cttcagcgcg tccaggtaat cggccacgca tagcatgctg ggcgtgttga
     1741 tggtgtcgcc
//
LOCUS       DAOMVO010000436         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_19866_length_1749_cov_19.099764, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000436 DAOMVO010000000
VERSION     DAOMVO010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19866_length_1749_cov_19.099764"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /gene="rbfA"
                     /locus_tag="PKV30_13780"
     CDS             <1..339
                     /gene="rbfA"
                     /locus_tag="PKV30_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRL31322.1"
                     /translation="VADQIQRDLSELIARELKDPRVGMVTINAVEVTPDYAHAKVFFS
                     LLNGDPEETATGLNAAAGFLRNGLFKRLHIHTVPTLHFIFDRTTERAADMNALIAKAV
                     ASRSKDDETP"
     gene            336..1322
                     /gene="truB"
                     /locus_tag="PKV30_13785"
     CDS             336..1322
                     /gene="truB"
                     /locus_tag="PKV30_13785"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRL31323.1"
                     /translation="MTATPRARVQRRPVHGVLLLDKPLGLSSNQALQKAKWLLRAEKA
                     GHTGTLDPLATGVLPLCFGAATKFSGLHLDADKTYEATLQLGVTTNTGDAEGEVIERR
                     AVPAITPEQLEAVRQRFLGRITQVPPMHSALKKDGKALYEYARAGIEVERAPREVAIH
                     ALNLALAQVDQAQAAIKIEVTCSKGTYIRTLGEDIGQALGCGGHLTRLRRTATGPFVA
                     AQCTTLPALEALDDHERLNLLQPAEALLPDHTAVTLAADDAAKFLTGLRRRGDWVDAD
                     HVVVFGDAPRALLGSAHIKAGELIPSRLLSPIEIRQILQAADARLHTQEVIA"
     gene            1319..>1749
                     /locus_tag="PKV30_13790"
     CDS             1319..>1749
                     /locus_tag="PKV30_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL31324.1"
                     /translation="MTQQIRNIAIIAHVDHGKTTMVDQLLRQSGTFASHEKVVDTVMD
                     NNAIERERGITILAKNCAVSWKGTHINILDTPGHADFGGEVERALSMVDGVVLLIDAQ
                     EGPMPQTRFVTKKALALGLKPIVVVNKVDKPGARPDYVINAA"
BASE COUNT          339 a          621 c          539 g          250 t
ORIGIN      
        1 gtcgccgacc agatccagcg cgatctgtcg gagttgatcg cgcgcgagtt gaaggacccg
       61 cgcgtcggca tggtcacgat caacgcggtc gaggtcacgc ccgattacgc acacgccaag
      121 gtgttcttca gcctgctgaa cggcgacccc gaggaaacgg ccaccggtct caacgccgcc
      181 gccggttttc tgcgcaacgg cttgttcaag cgcctgcaca tccacaccgt gcccacgctg
      241 cacttcatct tcgaccgcac caccgagcgc gcggccgaca tgaacgcgct catcgccaag
      301 gcggtggctt cgcgctcgaa agacgacgag acgccatgac cgccacccct cgtgcacggg
      361 tgcagcggcg cccggtgcat ggggtgttgc tgctcgacaa accgctgggc ctgtccagca
      421 accaggcgct gcaaaaagcc aagtggctgc tgcgcgccga aaaagccggc cacaccggca
      481 cgctcgatcc gctggccacc ggcgtgctgc cgctgtgctt tggcgccgcc accaagttca
      541 gcggcctgca tctggacgcc gacaagacgt atgaggccac gctgcaactc ggcgtgacca
      601 ccaacacggg cgacgccgaa ggcgaggtga tcgagcgccg cgccgtgcct gccatcacgc
      661 ccgagcagtt ggaagccgtg cgccagcgct ttctgggccg catcacgcaa gtgccgccga
      721 tgcacagtgc gctcaagaag gacggcaagg cgctgtacga gtacgcgcgc gccggcatcg
      781 aggtcgagcg cgcgccgcgc gaggtggcga ttcatgcatt gaatctggcg ctggcgcagg
      841 tggatcaagc gcaggcagct atcaaaatag aagtgacgtg cagcaaaggc acctacatcc
      901 gcacgctggg cgaagacatt ggccaggcgc tgggctgtgg cggccacctg acgcgcctgc
      961 gccgcaccgc caccggcccg ttcgtggccg cacagtgcac cacgctgccc gcgctggaag
     1021 cgctggacga ccacgaacgc ctgaaccttt tgcagccggc cgaagcgctg ctgcccgatc
     1081 acaccgccgt cacgctcgcc gccgatgatg cagccaagtt cctcaccggc ctgcgccggc
     1141 gcggcgactg ggtcgacgcc gaccacgtgg tcgtctttgg cgacgcaccc cgcgcactgc
     1201 tcggcagtgc gcacatcaag gcgggcgagc tgattccgtc gcgcctgctc agccccatcg
     1261 aaatccgtca aatcctccag gccgccgacg cgcggctgca cacccaagaa gtcatcgcat
     1321 gacccagcaa atccgcaaca tcgccatcat cgcccacgtt gaccacggca agaccaccat
     1381 ggttgaccaa ctcctgcgtc agtccggcac ctttgccagc cacgaaaaag tggtggatac
     1441 cgtgatggac aacaacgcca tcgagcgcga gcgcggcatc accatcctgg ccaagaactg
     1501 cgccgtgtcg tggaagggca cgcacatcaa catcctcgac acgcctggcc acgccgactt
     1561 tggtggcgag gtggagcgcg cgctcagcat ggtcgatggc gtggtgctgc tgatcgacgc
     1621 gcaggaaggc cccatgccgc aaacgcgctt cgtcaccaag aaggcgctgg cgctgggcct
     1681 gaaacccatc gtcgtcgtca acaaggtcga caagcccggc gcgcggcccg actacgtgat
     1741 caacgccgc
//
LOCUS       DAOMVO010000437         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_19867_length_1749_cov_10.774498, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000437 DAOMVO010000000
VERSION     DAOMVO010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19867_length_1749_cov_10.774498"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKV30_13795"
     CDS             complement(<1..1171)
                     /locus_tag="PKV30_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRL31325.1"
                     /translation="MTNITVADFANELKRPTDTLMEQLQAAGVSKQSASDALTDADKQ
                     KLLNYLQASHGTDASARRKITLTKKTTSEIKQADATGKARTIQVEVRKKRTFIKRDDE
                     PAAPAPAPMPEEVEAPAPVAATPAAPLIDAAEQARRDDEARRQSELMRRQEEEAAEKR
                     RQREENERRSAESASPASTDDAPAQPAPEPTAEPAVVAEAPAPSPAEAPAPVAPSPQA
                     EAERAREQEANRRKALAEAEAIRAMMSAPRKVLVAKKPEEKKADAAAKGTLHKPAGTA
                     AAKTGAGAPSANKEVKSAKLSSSWAGDPAKKKGIPTRGDSSGGVGRNNWRGGPRGRRG
                     ERGREEHATAQAPVEQRVIEVHVPETITVAELAHKMAIKASEVIKALMKMGQMVTI"
     gene            complement(1195..>1749)
                     /locus_tag="PKV30_13800"
     CDS             complement(1195..>1749)
                     /locus_tag="PKV30_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRL31326.1"
                     /translation="VDEENLAIAIGRGGQNVRLASELTGWKINIMDADESAQKQQAET
                     GQVRQLFMEKLDVDEEIADILIEEGFTSLEQIAYVPIEEMRDIESFDEETVGELRARA
                     KDALLTMDIAREENMEAVSQDLRDLEGLTAELIGKLSQAGVQTRDDLADLAVDELTDI
                     TGQSEEDARALILKAREHWFADQE"
BASE COUNT          187 a          584 c          580 g          398 t
ORIGIN      
        1 tgatggtgac catctggccc atcttcatca gcgccttgat gacctcggac gccttgatcg
       61 ccatcttgtg cgccagctcg gccacggtga tggtctctgg cacgtgcact tcgatcacgc
      121 gttgctcgac cggcgcctgg gccgtggcgt gctcttcacg cccacgctcg ccccggcggc
      181 cacgcgggcc gccacgccag ttgttgcggc cgacaccgcc cgagctgtcg ccacgggtgg
      241 ggatgccctt cttcttggcc ggatcgccgg cccagctgga cgacagcttg gcggacttga
      301 cttccttgtt ggccgatggc gcgccggcgc cggtcttggc ggccgcggtg cccgcgggct
      361 tgtgcagcgt gcccttggcg gcggcgtcgg cctttttctc ttccggcttc ttggcgacca
      421 gcaccttgcg tggcgcgctc atcatggcgc ggatggcctc ggcctcggcc agcgccttgc
      481 ggcggttggc ctcttgttcg cgggcgcgct cggcttcggc ctgcgggctc ggggcgacgg
      541 gtgcgggcgc ctcggcgggc gacggcgcgg gcgcttctgc caccacggca ggctcggcgg
      601 tcggctcagg cgcgggctgc gccggcgcat cgtcggtcga tgcggggctg gcgctttcag
      661 cgctccggcg ctcgttttct tcgcgctggc ggcgtttttc ggccgcttct tcttcctggc
      721 ggcgcatcag ttcggactgg cgacgtgcct catcgtctcg gcgcgcttgt tcggcggcgt
      781 cgatcagtgg cgcggcgggc gttgcggcaa cgggtgcggg cgcttccacc tcttccggca
      841 tcggagccgg tgcgggagcc gccggctcgt cgtcgcgctt gatgaaggtt ctcttcttgc
      901 gcacttcaac ctgaatggtg cgcgccttgc cggtggcgtc ggcctgcttg atctcgctgg
      961 tggtcttctt ggtcagcgtg atcttgcggc gcgcagacgc gtcggtgccg tggctggcct
     1021 gcaggtaatt cagcagcttt tgcttgtcgg catcggtcag cgcgtcggac gccgactgct
     1081 tgctgacgcc cgccgcctgc agctgctcca tcagcgtgtc agtgggccgt ttgagttcgt
     1141 tcgcgaaatc ggcaacagtg atattcgtca tatgcggtgc ttgtctccat acccttactc
     1201 ttgatcggcg aaccagtgct cgcgcgcctt caagatcagg gcgcgggcgt cctcttcgga
     1261 ctgcccggtg atgtcggtca attcatccac ggccaaatcg gccagatcgt cacgcgtttg
     1321 cacgccggct tgcgacagct tgccgattaa ttcggccgtc aggccttcca gatcgcgcaa
     1381 gtcctgcgac acggcttcca tgttttcctc gcgcgcgatg tccatggtca gcagcgcgtc
     1441 cttggcgcgg gcgcgcagct cgcccactgt ctcttcgtcg aaggattcga tgtcgcgcat
     1501 ctcttcgatg ggcacgtagg cgatctgctc caggctggtg aagccttcct cgatcaggat
     1561 gtcggcgatt tcctcgtcca catccagctt ttccatgaac agctggcgaa cctggccggt
     1621 ctctgcctgc tgcttctggg ccgattcgtc ggcgtccatg atgttgatct tccagccggt
     1681 cagctcggac gccagtcgca cgttctggcc gccgcggcca atggcgatgg cgaggttttc
     1741 ctcgtccac
//
LOCUS       DAOMVO010000438         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_19989_length_1741_cov_15.934757, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000438 DAOMVO010000000
VERSION     DAOMVO010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19989_length_1741_cov_15.934757"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKV30_13805"
     CDS             complement(<1..205)
                     /locus_tag="PKV30_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRL31327.1"
                     /translation="MTDANAIHTSQLHSLPLLARGKVRDNFAVGDDRILMVASDRISA
                     FDVIMGEPIPGKGQLLTQMALWWF"
     gene            complement(248..1618)
                     /locus_tag="PKV30_13810"
     CDS             complement(248..1618)
                     /locus_tag="PKV30_13810"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HRL31328.1"
                     /translation="MAMRLLKALKRSGFWLRERRNRLWVTPALASVVAVLLALAAAWV
                     SRVVPEDTLPDIDRDTVQSLLTIVASSMLAVTTFSLSIMVSAFASAANGATPRATELV
                     MGDEGTRSAIAMFLSAFIYAVVARVALGMGYYGGAGRFVLFLGTMGVLVMLLVTLVNW
                     VKTLSTLGRMSNTLNKIEQAAEKALRTHWRAPLLGAGPAPAVDAAPCGRPVYARQVAY
                     VRRIDLAALQDWAEANEARVHVRVRPGSFVDPGTELAWVELGIAQDQVVSATGGDEAA
                     HRGDRRSASDPDGESLRNVHDAFYLGAERSFDQDPRFGVIVLSEAAQRALSSAVNDPG
                     TAIDAMNRMTRLLIETQQDEQSLPHLPAVARLHTRVTLVPLDESALVFDGFDPIVRDA
                     AGVVQVLVRQQKLLAMIARSSRAGTVARAARDMAARALARADATGALPANERDALRAV
                     HDQLFN"
BASE COUNT          295 a          576 c          607 g          263 t
ORIGIN      
        1 cgaaccacca cagcgccatc tgcgtcagca actggccctt gccgggaatc ggctcgccca
       61 tgatgacgtc gaacgcgctg atgcggtccg acgccaccat aaggatgcgg tcgtcgccca
      121 cggcgaagtt gtcgcgcacc ttgccgcgcg caagcagggg caggctgtgg agctggctgg
      181 tgtgaatggc gttggcgtcg gtcatggtgg gagattggcc ggcagcggcg cgggggcgcc
      241 caggacatca gttgaacaat tgatcatgca cggcgcgcag cgcgtcgcgc tcgtttgcgg
      301 gcagcgcgcc ggtggcgtct gcgcgcgcga gggcccgcgc ggccatgtca cgcgcggcac
      361 gggccacggt gccggcgcgg ctgctgcggg cgatcatggc cagcagcttc tgctggcgca
      421 ccagcacttg caccacgccg gccgcatcgc gcacgatcgg gtcgaagccg tcgaacacca
      481 gggcggactc gtccagcggc accagggtga cgcgggtgtg cagtcgagcc acggcgggca
      541 ggtgcggcag ggactgctcg tcctgctggg tctcgatcag caggcgcgtc atgcggttca
      601 tggcgtcgat ggcggttccg gggtcgttga cggcgctgga cagggcacgc tgcgcggcct
      661 cggacagcac gataacgccg aagcgcgggt cctgatcgaa gctgcgctcg gcgccgaggt
      721 agaacgcatc atgcacgttg cgcagcgatt cgccgtcggg gtcgctggcg ctgcggcggt
      781 cgccgcgatg tgcagcctcg tcgccgccgg tggcgctgac aacctgatct tgcgcaatac
      841 caagctccac ccacgccagt tcggttccgg gatcgacaaa gctgccgggg cgcacacgca
      901 cgtgcacccg ggcttcgttg gcctcggccc agtcttgcag cgcggccaag tcgatgcgcc
      961 gcacataggc cacctgccgc gcatacacgg ggcggccgca cggtgcggca tcgacggcgg
     1021 gggcggggcc ggcgccgagc aggggcgccc gccagtgggt gcgcagcgcc ttttctgcgg
     1081 cctgctcgat cttattcaag gtgttggaca tgcgacccag cgtggacagc gtcttgaccc
     1141 agttcacgag cgtcacgagc agcatcacca gcacacccat cgtgcccaga aaaagcacaa
     1201 agcgcccggc gccgccgtag tagcccatgc caagggccac gcgcgccacc acggcataga
     1261 tgaaggccga cagaaacatg gcgatggccg agcgcgtgcc ttcgtcgccc atcaccagtt
     1321 cggtggcgcg cggtgtggcg ccattggctg ccgaggcgaa agccgacacc atgatcgaca
     1381 gcgagaacgt ggtcaccgcc agcatgctgg aggcgacgat ggtcagcagg ctttgcaccg
     1441 tgtcgcggtc gatgtcggga agcgtgtctt cagggacgac gcgcgacacc caggcggcgg
     1501 ccagcgccag tagcaccgct accacgctgg ccagcgcggg tgtcacccac aaccggttgc
     1561 gccgctcgcg cagccagaac ccgctgcgct tgagcgcctt cagcaagcgc atcgccatcg
     1621 ataaagctat cttttatata gctgctagcg cttgatggga aagcgctagg tgacgatttg
     1681 agccagatcg ccagcggcat agcgctcggc catcaccgac agcggcacgc ccttgatctt
     1741 g
//
LOCUS       DAOMVO010000439         1736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20093_length_1736_cov_15.680547, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000439 DAOMVO010000000
VERSION     DAOMVO010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20093_length_1736_cov_15.680547"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKV30_13815"
     CDS             <1..614
                     /locus_tag="PKV30_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL31329.1"
                     /translation="EYFIQQPSNPYAGYALKWDGEWQITYETFRDMGAAYAVGLILIY
                     LLVVAHFGSYLMPLIIMAPIPLTVIGVMPGHALLGAQFTATSMIGMIALAGIIVRNSI
                     LLVDFIHLQEAEGVPFKQAIVQSAITRAQPIALTALAAMIGALFILDDPIFNGLAIAL
                     IFGILVSTVLTLVVIPILYYVAYRRSHESNQAPAPVPPAQAAT"
     gene            630..836
                     /locus_tag="PKV30_13820"
     CDS             630..836
                     /locus_tag="PKV30_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRL31330.1"
                     /translation="MTSWQLTRLIGGTFILLSLALGVPGSPIFHSTNWLWFTAFVGAN
                     FLQSGITNWCLMETIMRKIGARAS"
     gene            852..1205
                     /locus_tag="PKV30_13825"
     CDS             852..1205
                     /locus_tag="PKV30_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRL31331.1"
                     /translation="MVENYKELIASVSANTAKLRKGIPDVMQGFNAMAGAAGKDGVLD
                     AKTKELIAMALSVAARCDPCVGYHAKALVKLGATRAELDEMLGMCVYMGGGPSLMYAA
                     AAIAAYEEFGGEKAA"
     gene            1202..>1736
                     /locus_tag="PKV30_13830"
     CDS             1202..>1736
                     /locus_tag="PKV30_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRL31332.1"
                     /translation="MSMQPAWVRRGLAIGLLGLAALPATAQDLLAVWRAAEQHDRTLA
                     VARAEHAAAQTKREQAEALWRPNVMLGLGAGLGASDTRMKGAQFSAPGFGTSEGVDFA
                     TSVHAGLATRANLIAQQPLVNRWRDAARAQLHLGAEMGDTAWRAARNELLLRTAERYF
                     ALAVAQEQLRVTERQAES"
BASE COUNT          288 a          611 c          551 g          286 t
ORIGIN      
        1 acgagtactt catccagcag ccctccaacc cctacgccgg ctacgcgctc aagtgggacg
       61 gcgagtggca gatcacctac gagaccttcc gcgacatggg cgccgcctac gccgtcggcc
      121 tgatcctgat ctacctgctg gtggtggcgc acttcggctc gtacctcatg ccgctgatca
      181 tcatggcgcc gattccgctc accgtcatcg gcgtgatgcc ggggcacgcg ctgctgggcg
      241 cgcagttcac cgccaccagc atgatcggca tgatcgcgct ggccggcatc atcgtgcgca
      301 actcgatcct gctggtcgac ttcatccacc tgcaggaggc cgagggcgtg ccgttcaagc
      361 aggccatcgt gcaaagcgcc atcacgcgcg cgcagccgat tgcgctgacg gcgctggccg
      421 ccatgatcgg cgcgctgttc atcctcgacg acccgatctt caacggcctg gccattgcgc
      481 tgatcttcgg catcctggtg tccaccgtgc tcacgctggt ggtcatcccc atcctctact
      541 acgtcgccta ccgccggagt catgagtcaa atcaggctcc agcgcccgtc ccgcctgcgc
      601 aagcagctac ttgaaaagga gcaaacagaa tgacatcgtg gcaacttacc cgcctcatcg
      661 gcggtacctt catcctgctt tcgctggcgc tgggcgtgcc cggcagcccc atcttccatt
      721 cgacgaactg gctgtggttc accgccttcg tcggcgccaa cttcctgcaa agtggcatca
      781 ccaactggtg cctgatggaa accatcatgc gcaagatcgg cgcccgtgca tcttgattca
      841 aggagaaacc catggttgaa aactacaagg aactgatcgc ctccgtatcc gccaacaccg
      901 ccaagctgcg caagggcatc cccgatgtca tgcagggctt caacgccatg gcgggcgctg
      961 ccggcaagga cggcgtgctg gacgccaaaa ccaaggaact aatcgccatg gcgctgtcgg
     1021 tggccgcgcg ctgcgacccg tgcgtgggct accacgccaa ggcgctggtc aagctgggcg
     1081 ccacacgcgc cgaactggat gagatgctgg gcatgtgcgt ctacatgggt ggcggcccgt
     1141 cgctgatgta cgccgccgcg gccattgccg cctacgagga gttcggcggc gagaaggccg
     1201 catgagcatg caacccgcct gggtgcgtcg cgggctggcc atcggcctgc tggggctggc
     1261 cgcgctgccg gccaccgcgc aggacctgct cgccgtgtgg cgcgctgccg agcagcacga
     1321 ccgcacgctg gccgtggccc gtgccgagca cgcggcggcg cagaccaagc gcgaacaggc
     1381 cgaggcgctg tggcgcccca acgtgatgct gggcctgggc gccggcctgg gcgccagcga
     1441 cacgcgcatg aagggcgcgc agttctcggc gcccggcttc ggcacgtccg agggcgtgga
     1501 cttcgccacc tcggtgcacg cgggactggc cacgcgcgcc aacctcattg cgcagcagcc
     1561 gctggtcaat cgttggcgcg acgccgcccg tgcgcaactt catctgggcg ctgagatggg
     1621 cgacacggcc tggcgcgccg cccgcaacga actgctgctg cgcaccgccg agcgctactt
     1681 tgccctcgcc gtggcgcagg agcagttgcg cgtgaccgag cgccaggccg aatcgg
//
LOCUS       DAOMVO010000440         1734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20129_length_1734_cov_11.488386, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000440 DAOMVO010000000
VERSION     DAOMVO010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20129_length_1734_cov_11.488386"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PKV30_13835"
     CDS             <1..1343
                     /locus_tag="PKV30_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL31333.1"
                     /translation="IHVVRDRLRTGFEKIDRVWSEVTNVLADTIPGIRVVKAFAQEER
                     EAGRFKEANRHNLAVNDKLNKLWSLFSPTVTLLTEIGLLVVWAAGIWLIAHQRITVGV
                     LTAFLAYIGRFYTRLDSMSRIVSVTQKAASATKRIFEILDHESSVPEPKNPVPLTHVE
                     GRIEVKNAALRYGNRSVIRGLNLTIEPGEMIGLVGHSGSGKSTMVNLICRFYDVTEGA
                     ILVDGIDVRKVRVGDYRKHVGLVLQEPFLFFGTIAENIAYGKPDATREEIVAAARAAH
                     AHEFILRLPQGYDSLVGERGQGLSGGERQRISIARALLINPRILILDEATASVDTETE
                     REIQKALDNLVRGRTTIAIAHRLSTLRKADRLVVMDKGLKVEEGTHEQLIAQRGAYWR
                     LYEAQARQEKAERERMLEIDGPALATDAPPAPVPAQPHIDREAEVEASLKEQRP"
     gene            1340..>1734
                     /locus_tag="PKV30_13840"
     CDS             1340..>1734
                     /locus_tag="PKV30_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HRL31334.1"
                     /translation="MSAAATIFELRRDAFGRLVLTDAQGAEHVGVLAVRAFPIHAADE
                     GIALVDGDGHELAWVPRLDQLDEPRRALLAEALEQREFMPELRRVHGVSSFATPSTWD
                     VDTDRGRTQFVLKGEEDIRRLGHGLLIVN"
BASE COUNT          310 a          605 c          568 g          251 t
ORIGIN      
        1 tgatccacgt ggtgcgcgac cgcctgcgca ccggcttcga gaagatcgac cgcgtgtgga
       61 gcgaggtgac caacgtgctg gccgacacca ttccgggcat ccgcgtggtg aaggcgtttg
      121 cgcaggaaga gcgcgaggcc ggccgcttca aagaggccaa ccgccacaac ctggccgtca
      181 acgacaagct caacaagctg tggtcgctgt tttcgcccac cgtcacgctg ctgaccgaaa
      241 tcggcctgct ggtggtgtgg gcggccggca tctggctgat tgcgcaccag cgcatcaccg
      301 tcggtgtgct caccgccttt ctggcctaca tcgggcgctt ttacacccgg ctggattcga
      361 tgagccgcat cgtctcggtg acgcaaaaag ccgcctcggc caccaagcgc atcttcgaga
      421 tcctcgacca cgaatccagc gtgccggagc cgaaaaaccc ggtgccgctg acgcacgtcg
      481 aaggccgcat cgaagtgaaa aacgccgcgc tgcgctacgg caaccgctcg gtgattcgcg
      541 gcctgaacct gaccatcgag ccgggcgaga tgatcggcct ggtcggccac agcggctcgg
      601 gcaagagcac catggtcaac ctgatctgcc gcttctacga cgtgaccgaa ggcgcgatcc
      661 tggtcgacgg catcgacgtg cgcaaggtgc gtgtgggcga ctaccgcaag cacgtcggtc
      721 tggtgctgca agagcctttc ctcttcttcg gcaccattgc cgagaacatc gcctacggca
      781 agcccgatgc gacgcgcgaa gaaatcgttg ccgccgcgcg cgccgcgcac gcgcacgaat
      841 tcatcctgcg cctgccgcag ggctacgact cgctggtggg cgagcgcggc cagggcctgt
      901 cgggcggcga gcgccagcgc atcagcattg cgcgcgcgct gctgatcaac ccgcgcatcc
      961 tgatcctgga cgaagccacc gcctccgtcg ataccgagac cgagcgcgag atccagaagg
     1021 cgctcgacaa tctggtgcgc gggcgcacca ccatcgccat cgcgcaccgc ctgtccacgc
     1081 tgcgcaaggc ggatcgtctt gtggtgatgg acaagggcct gaaggtggaa gagggcacgc
     1141 acgagcagct catcgcccag cgcggcgcct actggcgcct gtacgaagcg caggctcggc
     1201 aagaaaaggc ggagcgcgaa cgcatgctcg aaatcgacgg ccccgcgctg gccaccgacg
     1261 ctccccctgc gcccgtgccc gcgcagccac acatcgaccg cgaggccgag gtcgaagcca
     1321 gcctcaagga gcagcgccca tgagcgccgc cgccaccatc ttcgagctgc gccgcgacgc
     1381 ctttggccgc ctggtgctga ccgacgcgca aggcgccgag cacgtcggcg tgctggccgt
     1441 gcgcgccttt ccgatccacg ccgccgatga aggcattgcg ctggtcgacg gcgacggcca
     1501 cgagctggcc tgggtgccgc gcctggatca actggacgag ccgcgccgcg cgctgctggc
     1561 cgaggcgctg gagcagcgcg aattcatgcc cgagctgcgc cgcgtgcacg gcgtcagcag
     1621 ctttgccacg cccagcacct gggacgtgga caccgaccgc ggccgcacgc agttcgtgct
     1681 gaagggcgag gaagacatcc gccgcctggg gcacggcctg ctgatcgtca acga
//
LOCUS       DAOMVO010000441         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20356_length_1720_cov_22.939940, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000441 DAOMVO010000000
VERSION     DAOMVO010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20356_length_1720_cov_22.939940"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="ppk2"
                     /locus_tag="PKV30_13845"
     CDS             complement(<1..983)
                     /gene="ppk2"
                     /locus_tag="PKV30_13845"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRL31335.1"
                     /translation="MDKSSPRKTPAKRPKAAPAAINDAVRAVKKGDTPPSLAPKAIEN
                     YVVERATVSARDKQLAAALRLVNDEAAGTPEERAHGLRAIIEGASPDDRKALRKALSA
                     NGQDAHEDTPNPDEELAPDWRDGGYPYKNLLRRSTYEKQKFKLQVELLKLQSWVKETG
                     ARVVIIFEGRDAAGKGGTIKRFMEHLNPRGARTVALEKPSDVERGQWYFQRYVQHLPS
                     FGEIVLFDRSWYNRAGVERVMGFCSDKEYQDFMRQAPEFERHLVSSGVHLIKFWFSVS
                     QAEQRRRFKEREGHPLKQWKLSPIDKASLDKWDDYTRAKEQMFFETDTADSP"
     gene            complement(1090..1680)
                     /locus_tag="PKV30_13850"
     CDS             complement(1090..1680)
                     /locus_tag="PKV30_13850"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRL31336.1"
                     /translation="MSKFDKKAVRERLVQERLHLEDRLARADALQDILRIWLIDRPDT
                     VIGAYWPIKGEFDPLPALHRWKEDGELMDEPQRRRIGLPVIDKVHKTLTFHAWYPGCP
                     TEEDAYGIPKPKDTELIVPTLLFVPCVGYGPGGYRLGYGGGFYDRTLAALEPRPFTVG
                     VGFTNGFIDDFQPEPHDQPLDAILNDNGVVWPITRL"
BASE COUNT          238 a          558 c          573 g          351 t
ORIGIN      
        1 gggctgtcgg cggtgtcggt ctcgaagaac atctgctcct tggcgcgggt gtagtcgtcc
       61 cacttgtcca ggctggcctt gtcgatgggc gacagcttcc actgcttcag cgggtggcct
      121 tcgcgctcct tgaagcggcg gcgctgctcg gcctggctga cgctgaacca gaacttgatg
      181 agatgcacgc cgctgctcac caggtgccgc tcaaattcgg gcgcctggcg catgaagtcc
      241 tggtattcct tgtcactgca aaagcccatc acccgctcga cgccggcgcg gttgtaccag
      301 ctgcggtcga acagcacgat ctcgccaaag ctcggcaaat gctgcacgta gcgctggaag
      361 taccactggc cgcgctcgac gtcgctgggt ttttccagcg ccacggtgcg cgcgccgcgt
      421 gggttcaggt gctccatgaa gcgcttgatg gtgccgccct tgccggccgc atcgcgccct
      481 tcaaagatga tgaccacgcg cgccccggtc tccttgaccc agctttgcag cttgagcagt
      541 tcgacctgga gcttgaactt ctgcttttcg tacgtcgagc ggcgcagcag gttcttgtac
      601 gggtagccgc cgtcgcgcca gtcgggcgcc agttcctcgt cggggttggg cgtgtcttcg
      661 tgcgcgtcct ggccgttggc gctgagcgcc ttgcgcagcg ccttgcggtc gtcgggcgag
      721 gcgccttcga tgatggcgcg caggccgtgc gcacgctctt cgggtgtgcc ggcggcctcg
      781 tcgttcacca gacgcagcgc ggcggccagt tgcttgtcgc gcgccgacac ggtggcgcgc
      841 tcgaccacgt agttctcgat tgccttgggc gccagcgaag gtggcgtgtc gcccttcttc
      901 accgcgcgca cggcgtcgtt gatcgcggcg ggcgcagctt tggggcgctt ggcaggggtc
      961 ttgcgggggc tgcttttgtc catgggtggg ttccttgatg cgccggcggt caacccggcc
     1021 gagccattca ctatccgcct gtcacggcgt cgtcatcttg acgcgcatca agaaacgcgg
     1081 cgtcgaattt cacagccgtg tgatcggcca caccacgccg ttgtcgttca ggatcgcgtc
     1141 cagcggctgg tcgtgcggct cgggctgaaa gtcgtcgatg aagccgttgg taaaacccac
     1201 gccgacggtg aacgggcgcg gctccagcgc cgccagcgtg cggtcgtaaa agccgcctcc
     1261 ataacccagc cggtagccgc ccggcccgta gcccacgcag ggcacgaaca gcagcgtcgg
     1321 cacgatcagc tcggtgtcct tgggcttggg gatgccgtag gcgtcttcct cggttgggca
     1381 gccggggtac caggcgtgga aggtgagcgt cttgtgcacc ttgtcgatca ccggcaggcc
     1441 gatgcggcgg cgctgcggct cgtccatcag ctcgccgtct tccttccagc ggtgcagcgc
     1501 gggcagcggg tcgaattcgc ccttgatcgg ccaataagcg ccaatcaccg tgtcgggccg
     1561 gtcgatcagc cagatgcgca ggatgtcctg cagcgcatcg gcccgcgcca gccggtcttc
     1621 caggtgcagg cgctcttgca ccaggcgctc gcgcacggct ttcttgtcga acttgctcat
     1681 aatgggtcga tgcgtttttc aaagattctg acaccgttgc
//
LOCUS       DAOMVO010000442         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20486_length_1712_cov_10.410380, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000442 DAOMVO010000000
VERSION     DAOMVO010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20486_length_1712_cov_10.410380"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKV30_13855"
     CDS             complement(<1..619)
                     /locus_tag="PKV30_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL31337.1"
                     /translation="MSTGAPADASLIDSRTAAWRLLVTVLLVTLGNSAMYVVSVVLPA
                     VQSEFGISRANASLPYTFMMVCLGLGGLFTGRWADRFGIARVLWLGSAAVLGGYVLAA
                     LSGNVWMFGLAHGLLGLLGGAATFAPLMADTGLWWNKRRGMAMAVCASGNYIAGTLWP
                     PLAQWGITHYGWRPTYTALGLVCGIGMALLAFYMRRPPPALALPAT"
     gene            complement(616..1095)
                     /locus_tag="PKV30_13860"
                     /pseudo
     CDS             complement(616..1095)
                     /locus_tag="PKV30_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871292.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1116..1433)
                     /locus_tag="PKV30_13865"
     CDS             complement(1116..1433)
                     /locus_tag="PKV30_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRL31338.1"
                     /translation="MTTTTLPNVTLTTQANVYFDGKCVSHGFTLADGTKKSVGVVLPA
                     TLTFNTGAAEIMECVGGACEYKLAGSDEWKKSGPGDKFSIPANSSFEIRVTEPYHYIC
                     HFG"
     gene            1583..>1712
                     /locus_tag="PKV30_13870"
     CDS             1583..>1712
                     /locus_tag="PKV30_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRL31339.1"
                     /translation="MQAPRTRFVLIDTSHAGNVGAVARAMKVMGFDDLVLVNPRWAD"
BASE COUNT          284 a          595 c          566 g          267 t
ORIGIN      
        1 ccgtggccgg cagcgccagc gcgggcggcg gccggcgcat gtaaaaggcc agcaacgcca
       61 tgccgatgcc gcacaccagc cccagcgcgg tgtaggtcgg ccgccagccg taatgcgtga
      121 tgccccattg cgccagcggc ggccacagcg tgccggcgat gtagttgccg ctggcgcaca
      181 ccgccatcgc catgccgcgc cgcttgttcc accacaggcc ggtgtcggcc atcagcggcg
      241 cgaacgtggc cgccccgccc agcaggccca gcagcccgtg cgccaggccg aacatccaca
      301 cattgcccga cagcgccgcc agcacatagc cgcccagcac ggccgccgac ccgagccaca
      361 gtacgcgcgc aatgccgaag cggtcggccc aacggccggt gaacagcccg cccagcccca
      421 ggcagaccat catgaaggtg tagggcagtg acgcattggc gcggctgatg ccgaattcgc
      481 tttgcaccgc cggcagcacc accgacacca cgtacatcgc gctgttgccc agcgtgacca
      541 gcagcaccgt caccagcagc cgccaggcgg cggtgcgcga atcgatcagg ctggcgtcgg
      601 ccggcgcgcc ggtgctcaca tatcaagctc ccagtgctcg ccgaccaggt cgtgcccgta
      661 gccgtgataa ggctcgcttg ctatcagttt gaaaccacgt tttgcataga tggcgcgcgc
      721 tgccagcaga ttggtgttgg tctacagcac cagcttggcg tagcccttgg cccgcgcgaa
      781 ggcgatgcat tcgtcggtca gccggccgcc caggccaagc ccgcgcgcgg cgggggtcag
      841 gatcaagagg cgcaactgcg cctcagtctc gctcttgcgc accacgaaga cggcgcccac
      901 gcgctcgccg tccagttcgg cgatccagcc gcgctcccac gccgcgtcgt gcgtgcggat
      961 catgccggcg gcgatctcgg ccaccagcgc ctcgaagtcg cggttccagc cgaattcgtc
     1021 ccagtacagc gcgccatgct gctggaccac ccagcccatg tcgccggggc gcgggtcgcg
     1081 cagcagcacg gtgcgggcgc gttcggtcat ggtgatcagc cgaagtggca gatgtagtgg
     1141 tacggctcgg tcacacgaat ctcgaacgac gagttggcgg ggatgctgaa tttgtcgccc
     1201 gggccggatt ttttccactc gtcgctgccg gccagcttgt attcgcaggc gccgcccacg
     1261 cattccatga tctcggccgc gccagtgttg aaagtcagcg tggcgggcag caccacgcca
     1321 acgctcttct tggtgccgtc ggccagcgtg aagccgtgcg agacgcattt gccgtcgaag
     1381 tagacgttgg cttgggtggt cagggtgacg ttgggcaggg tggtcgtggt catggtgagc
     1441 gtcaatcaat caaagccgcg atgttaccga tgcaggcgct ttgtgggagc ggccttggcc
     1501 gcgatgggcg cttccatggc cgatgcgccg ccatcgcggc caaggccgct cccacgaaca
     1561 tcgacccggc gacaatcggc cgatgcaggc tccgcgcacc cgttttgtat tgatcgacac
     1621 cagccacgcc ggcaacgtgg gcgcggtggc gcgcgccatg aaggtgatgg gctttgacga
     1681 cctggtgctg gtcaacccgc gctgggccga cg
//
LOCUS       DAOMVO010000443         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20498_length_1711_cov_27.588768, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000443 DAOMVO010000000
VERSION     DAOMVO010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20498_length_1711_cov_27.588768"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKV30_13875"
     CDS             complement(<1..409)
                     /locus_tag="PKV30_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRL31340.1"
                     /translation="MASKDPSSSSATRRIQVRRSGVHGKGVFALVDIARGERIIEYVG
                     EIISWDEAQRRHPHDPNDPNHTFYFHVDEDHVIDALYGGNASRWINHSCNPNCIADED
                     EGRIFITARRKIKAGDELNYDYGLVIDEPLTPEL"
     gene            516..>1711
                     /locus_tag="PKV30_13880"
     CDS             516..>1711
                     /locus_tag="PKV30_13880"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRL31341.1"
                     /translation="MKTLVIAEKPSVAQDIVRALTPVAGKFDKHAEHFENDRYVVTSA
                     VGHLVEIQAPEAFDVKRGKWSFAHLPVIPPHFDLKPVDKTKTRLNAVVKQARRKDVST
                     LINACDAGREGELIFRLIEQYAGGGKPLGKPVQRLWLQSMTPQAIRDGFENLRSDAQM
                     QGLADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLAVVVEREEQI
                     RRHIARDYWEVHATFVAEAGEYAGKWFDPAWKKDPEDADKRADRVWQEREALAIADAV
                     RGQACSVKEESKPTTQASPLLFDLTSLQREANGKFGFSAKTTLALAQSLYERHKALTY
                     PRTDARYLPEDYVPVARKTFEMLAHSNQKHLAPHAAKALADGYIKPTKRIFDNSKVSD
                     HFAIIPT"
BASE COUNT          320 a          550 c          569 g          272 t
ORIGIN      
        1 tcagctcggg cgtcagcggc tcgtcgatga ccaggccgta gtcgtagttc agctcatcgc
       61 ccgccttgat cttgcggcgc gcagtgatga agatgcggcc ttcgtcctcg tcggcaatgc
      121 agttggggtt gcagctgtgg ttgatccagc gcgaggcgtt gccgccgtac agcgcgtcga
      181 tcacgtggtc ttcatcgacg tggaaataga aggtgtggtt gggatcattc gggtcgtgcg
      241 ggtggcggcg ctgcgcttcg tcccagctga tgatttcgcc cacgtattcg atgatgcgct
      301 cgccgcgcgc gatgtcgacc agggcgaaca cgcccttgcc gtgcacgccc gagcggcgca
      361 cctggatgcg gcgcgtggcc gacgaacttg aagggtcttt gctggccacg aaaagttttt
      421 gctatgttgg atttgtgggc gcgcgcatgc aggggcgtgg gcgcgcacag gcgggtgcgc
      481 gtgtgcgcac gcgcgaggag ccggattcta gccacatgaa aacactggtc attgcagaaa
      541 aaccatcggt cgcgcaggac atcgtgcgcg ccctcacgcc ggttgccggc aagttcgaca
      601 agcacgccga gcatttcgag aacgatcgct acgtggtgac aagcgccgtc ggccacttgg
      661 tggagatcca ggcgcctgaa gccttcgacg tgaaacgcgg caagtggagc ttcgcccacc
      721 tgcccgtgat tccgccgcac ttcgacctga agccggtcga caagaccaag acgcgcctga
      781 acgccgtcgt caagcaggcg cggcgcaagg atgtgagcac gctcatcaac gcctgcgacg
      841 ccgggcgcga gggcgagctg atcttccgcc tgatcgagca gtacgccggt ggcggcaagc
      901 cgctgggcaa gccggtgcag cgcctgtggc tgcagagcat gacgccgcag gccatccgcg
      961 acggctttga aaacctgcga agcgacgcgc agatgcaggg cctggccgac gccgcgcgca
     1021 gccgcagcga ggccgactgg ctggtcggca tcaacggcac gcgcgccatg acggcgttca
     1081 actcgcgcga tggcgggttc tttttgacca ccgtgggccg tgtgcagacg ccgacgctgg
     1141 ccgtggtggt ggagcgcgag gagcagattc gacgccacat cgcgcgcgac tactgggaag
     1201 tgcacgccac ctttgtcgcc gaagcgggcg aatacgccgg caagtggttc gacccggcct
     1261 ggaagaaaga ccccgaagac gccgacaagc gcgccgaccg cgtgtggcag gaacgcgaag
     1321 cgctggccat tgccgacgcc gtgcgcggcc aggcctgcag cgtcaaggaa gaaagcaagc
     1381 cgaccacgca ggcctcgccg ctgctgttcg atctgacatc gctgcagcgc gaagccaacg
     1441 gcaagttcgg cttttccgcc aagaccacgc tggcgctggc gcaatcactt tatgagcgcc
     1501 acaaggccct gacctacccg cggaccgacg cgcgctacct gcccgaagat tacgtgccgg
     1561 tggcgcgcaa gaccttcgag atgctggcgc acagcaacca gaagcacctg gcgccccacg
     1621 ccgccaaggc gctggccgac ggctacatca agcccaccaa gcgcatcttc gacaacagca
     1681 aggtgtcgga ccacttcgcc atcatcccca c
//
LOCUS       DAOMVO010000444         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20703_length_1699_cov_11.843674, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000444 DAOMVO010000000
VERSION     DAOMVO010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20703_length_1699_cov_11.843674"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKV30_13885"
     CDS             complement(<1..1060)
                     /locus_tag="PKV30_13885"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRL31342.1"
                     /translation="MNAPDKFAQLLTLTREPFPGSTKTYLEGSRPDLRVPVRDIALTN
                     GAQASLYDTSGPYTDPQAQIDVRRGLPALRAAWIDERGDTESYEGRLAHILDDGGKHE
                     GRDLERIEQLRREAAGLQRQPRRAKAGGNVTQMHYARRGIITPEMEYIALRENGRLEW
                     TREHLSDPKREARRKGNPMGAVMPDVITPEFVRDEVARGRAIIPANINHPEIEPMIIG
                     RNFRVKINANIGNSAVTSSIEEEVEKLVWSIRWGGDTVMDLSTGKNIHTTRDWIIRNS
                     PVPIGTVPIYQALEKVGGIAEDLTWQIFRDTLIEQAEQGVDYFTIHAGVRLPFIHLTA
                     DRVTGIVSRGGSIMAKWCI"
     regulatory      complement(1137..1247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1250..>1699
                     /locus_tag="PKV30_13890"
     CDS             1250..>1699
                     /locus_tag="PKV30_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31343.1"
                     /translation="MIAGKPFALARDASDLVGECYELIGRRAELASPSITGRTDAYQD
                     LLLKVTPPRLPRNLVPRKRLTTTAHALGDASVLLVQAPAGFGKTSLLAQWRKEIQSTG
                     VAVAWLSAHTRDDPHRLVQALTLAVRVASGKAAFGQAAFEFDGDDRLE"
BASE COUNT          233 a          530 c          589 g          347 t
ORIGIN      
        1 cgatgcacca cttggccatg atggagccgc cgcggctgac gatgcccgtc acgcgatccg
       61 ccgtgaggtg gatgaagggc agacgcacgc ccgcgtggat ggtgaagtag tccaccccct
      121 gctccgcctg ctcgatcagc gtgtcgcgga agatttgcca ggtcaggtct tcggcaatgc
      181 cgcccacttt ttccagcgcc tggtagatcg gcacggtgcc gatgggtacg gggctgttgc
      241 ggatgatcca gtcgcgcgtg gtgtggatgt tcttgccggt cgacagatcc atcaccgtgt
      301 cgccgcccca gcggatcgac cagaccagtt tttcgacctc ttcctcgatg ctgctggtga
      361 cggccgagtt gccgatgttg gcgttgatct tcacgcggaa gttgcggccg atgatcatcg
      421 gctcgatttc cgggtggttg atgttggccg ggatgatggc gcggccgcgc gccacctcgt
      481 cgcgcacgaa ctcgggcgtg atcacgtcgg gcatcacggc gcccatgggg ttgcccttgc
      541 ggcgcgcctc gcgcttgggg tcactcaggt gttcgcgcgt ccattcgagg cggccgtttt
      601 cgcgcagcgc gatgtattcc atctcgggcg tgatgatgcc gcgccgcgca tagtgcatct
      661 gcgtgacgtt gccaccggct ttggcgcggc gcggctggcg ctgcaggcca gccgcttcgc
      721 ggcgcagttg ctcgatacgc tccagatcgc gcccttcgtg cttaccgcca tcgtcgagga
      781 tgtgggccag ccgtccttcg tagctctcgg tgtcgccgcg ctcgtcgatc cacgccgcgc
      841 gcagcgcggg caggccgcgc cgcacgtcga tctgcgcctg cgggtcggtg tagggacccg
      901 aggtgtcgta caggctggcc tgcgcgccgt tggtgagcgc aatgtcgcgc accggcacgc
      961 gcaggtcggg ccgcgatcct tcaaggtagg tcttggtcga acctggaaat ggctcgcgcg
     1021 tgagcgtgag gagttgggcg aacttgtcgg gcgcgttcat cggcgggtct cctgtggtgt
     1081 ctgtgtttcg actccggggc cgcctgccgt gccatgaccg cttgaagggt cggtggcgcc
     1141 gtgggtgctg ctcttcctta cgccggtcct aaccggatca agttgacggg ttcggctgcc
     1201 agatcagcca tctcagcgcg catgtgcggg caccccggag tgtgggcgga tgatagccgg
     1261 taaaccgttc gcgctggcgc gtgatgcgtc tgatcttgtt ggagaatgct acgaactcat
     1321 tggcaggcgc gcagaattgg caagtccatc aatcaccggc aggacggacg cttatcagga
     1381 ccttcttctc aaggtcacgc cgccgcgcct gccgcgcaac ttggtgcctc gaaagcgcct
     1441 gaccaccacc gcccatgcgc tgggcgatgc gtcggttctg ctggtacagg caccggcggg
     1501 gtttggaaag acctccttgc tggcccagtg gcgcaaggag atccagtcga ccggtgtggc
     1561 tgtcgcgtgg ctgtccgcgc acacgcgtga cgatccacac cggctggtgc aggccctgac
     1621 cctggcggtg cgcgtggcat ctggcaaggc ggccttcggc caggccgcct tcgagtttga
     1681 tggtgacgac cggcttgag
//
LOCUS       DAOMVO010000445         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20741_length_1697_cov_19.669306, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000445 DAOMVO010000000
VERSION     DAOMVO010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20741_length_1697_cov_19.669306"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PKV30_13895"
     CDS             complement(<1..1015)
                     /locus_tag="PKV30_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRL31344.1"
                     /translation="MIDGFNRHYTLFRTESARAKHRFETCDWHGQQRAQRERIEFYDL
                     RVRECQRRLEREFKAGEQPMDIWQQVKLHYIGLLVAHQQPELAETFFNSVTTKILHRS
                     YFHNDFIFVRPAISTEYLETDDPKAKTTYQAFYPAVDKLEDSVRAVLESFALHCDFED
                     IGRDARCVASAMRRELGDARARPNFQIQVLTSLFYRNKGAYLVGKLINGFREVPFALP
                     ILHRTPGTVYVDAALFGEEDLLILFSFSRAYFMVDMEIPSAYVQFLRSLMPRKPRAEI
                     YNALGLAKQGKNLFYRDFLWHLRHSTDQFRIAPGIKGMVMLVFDLPSFPYVFKVIKDW
                     YPPP"
     gene            complement(1069..1683)
                     /locus_tag="PKV30_13900"
     CDS             complement(1069..1683)
                     /locus_tag="PKV30_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRL31345.1"
                     /translation="MSAQQSPKYLWIGCSDSRVPANQVIGLAPGEVFVHRNVANVVAH
                     SDLNALSVIQFAVDVLKVEHIMVVGHYGCGGVLAVLHEKRVGLADNWLRHVNDVKMRH
                     RGRLMHLCGTAQEDALCEMNVIEQVGNIAQTNIAQDAWKRGQPLAIHGWCYGLKDGLV
                     KDLGVTMSHADQVVDVFRAAIKRYPRSDAVAAEFGATEPPPDES"
BASE COUNT          297 a          527 c          565 g          308 t
ORIGIN      
        1 tgggcggcgg ataccagtcc ttgatgacct tgaacacgta gggaaagctg ggcaagtcga
       61 acaccagcat caccatgccc ttgatgccgg gcgcgatgcg gaactggtcg gtgctgtgac
      121 gcaggtgcca cagaaagtcg cggtagaaca ggttcttgcc ttgcttggcc agcccgagcg
      181 cgttgtagat ttcggcgcgc ggcttgcgcg gcatgaggct gcgcagaaac tgcacgtagg
      241 ccgatggaat ctccatgtcg accatgaaat aggcgcgtga gaagctgaac agaatcagca
      301 ggtcttcttc gccaaacagc gccgcatcca cgtacaccgt gcccggcgtg cggtgcagga
      361 tgggcagtgc aaacggtact tcacgaaagc cgttgatgag cttgccgacc aggtaggcgc
      421 ccttgttgcg atagaacaga ctggtgagta cctggatctg aaagttgggc cgcgcgcgcg
      481 catcgcccag ctcgcgccgc atggcgctgg ccacgcagcg cgcgtcgcgc ccgatgtcct
      541 caaaatcgca gtgcagggca aacgattcca gcaccgcgcg caccgaatct tcgagcttgt
      601 ccactgcggg atagaaggcc tgataagtcg tcttggcctt ggggtcgtcg gtctccagat
      661 actcggtgct gatggccggg cgcacgaaga tgaagtcgtt gtggaagtag ctgcggtgca
      721 ggatcttggt ggtgaccgag ttgaagaagg tctcggccag ctcgggctgc tggtgcgcca
      781 ccagcaggcc gatgtagtgc agcttgacct gctgccagat gtccatcggc tgctcgccgg
      841 ccttgaactc gcgctcgagc cggcgctggc attcgcgcac gcgcaggtcg tagaactcga
      901 tgcgctcgcg ctgcgcgcgc tgctggccgt gccagtcgca ggtttcaaag cggtgcttgg
      961 cgcgcgccga ctcggtgcga aacagcgtgt agtggcggtt gaagccgtcg atcatcgccc
     1021 gcgcgatgtc gcgcgccagc ggtgaatcga gcgccgccag ctccacgctc agctttcgtc
     1081 aggcggcggt tcggtggcgc cgaattcggc cgcgacggcg tcgctgcgcg ggtagcgctt
     1141 gatggcggcg cgaaacacat cgaccacctg gtcggcgtgc gacatggtca cgccgaggtc
     1201 cttcaccagg ccgtccttca ggccatagca ccagccgtgg atggccagcg gctggccgcg
     1261 cttccaggcg tcctgcgcga tgttggtctg cgcgatgttg ccgacctgct cgatcacgtt
     1321 catctcgcac agcgcgtctt cctgcgccgt gccgcacagg tgcatcaggc ggccgcggtg
     1381 gcgcatcttg acgtcgttga cgtggcgcag ccagttgtcg gccaggccga cgcgcttttc
     1441 atgcagcacc gccagcacgc cgccgcagcc gtagtggccg accaccatga tgtgctcgac
     1501 cttcagcacg tcgaccgcga actggatgac ggacagcgcg ttcaagtcgg agtgcgcgac
     1561 cacgttggcc acgttgcggt gcacgaacac ctcgcccggt gccaggccga tgacctggtt
     1621 ggccggcacg cggctgtcgg aacagccgat ccacaaatac ttcggcgact gctgagccga
     1681 cagctccttg aagaagc
//
LOCUS       DAOMVO010000446         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20786_length_1694_cov_10.486272, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000446 DAOMVO010000000
VERSION     DAOMVO010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20786_length_1694_cov_10.486272"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PKV30_13905"
     CDS             <1..137
                     /locus_tag="PKV30_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRL31346.1"
                     /translation="AEVHQRIVFADSVAAGRLARETAPDSAAAREITALVDELLRWTP
                     "
     gene            134..406
                     /locus_tag="PKV30_13910"
     CDS             134..406
                     /locus_tag="PKV30_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HRL31347.1"
                     /translation="MTAKPPPRAKRVGIGARPPANPHAEAWIRQGDADALGKGDLYTA
                     RLTLDITPAMRARIKVSAFTQGVTVADLLRGLLEREFPEHRRENTP"
     gene            403..948
                     /locus_tag="PKV30_13915"
     CDS             403..948
                     /locus_tag="PKV30_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HRL31348.1"
                     /translation="MTASASSALAPVAIAATAALAAASGQPASTPLTRVALAYIEARF
                     KLYLRFGEPARTHQLDRWRRSAVFLPGAVFCRIRWQANDYGTVRWQLMVMQACTPLDV
                     AQRIPGVQPGARLLLHAEGDGQVRAVLERIDAIEALGIAPAAVSPAYWRTLGNRLAAR
                     LPLPEYTAERHAAWLTGRALS"
     gene            945..1544
                     /locus_tag="PKV30_13920"
     CDS             945..1544
                     /locus_tag="PKV30_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRL31349.1"
                     /translation="MTLPSAAAGATGIPPRPCSRLYARLVLAGLSACGLAAVAWASFV
                     HPLPRLIYNPSDSVAVGWYRVDPLGHGAGSLPRPLSVGSIVLTTLPPEAAALAAQRGY
                     LPARVPLLKRVGAVAPQTVCIFDALVWIDGVPMAAVLPADRLGRPLPAWPQCRRLEPG
                     ELFLLSETNPASFDSRYFGPVSAPAVIGVAHPIWLESRP"
BASE COUNT          205 a          634 c          570 g          285 t
ORIGIN      
        1 gcgcggaagt gcatcagcgc atcgtgttcg ccgacagcgt ggccgctggc cggcttgcac
       61 gcgagacggc gcccgacagc gccgccgcgc gcgaaatcac cgcgctggtg gacgaactgc
      121 tgcggtggac gccatgacag cgaagccgcc gccccgcgca aagcgcgtcg gcatcggcgc
      181 gcgtccgcct gcgaatccgc acgccgaggc gtggattcgc cagggcgacg ccgatgcgct
      241 gggcaaaggc gacctctaca cggctcgcct gaccctcgac atcacgcccg cgatgcgggc
      301 gcgcatcaag gtctcggcct tcacgcaggg cgtgaccgtg gccgacctgc tgcgcggcct
      361 gctggagcgc gagtttccag aacaccgcag ggagaacacg ccatgactgc atccgcttcg
      421 tctgccctcg cgcctgttgc catcgcggcc acggctgcgc tcgcagcagc ttccggccag
      481 cccgccagca cgccgctgac gcgcgtggcg ctggcctaca tcgaggcccg cttcaagctc
      541 tacctgcgct tcggcgaacc cgcgcgcacg caccagctcg accgctggcg gcgcagcgcg
      601 gtgttcctgc cgggcgccgt gttctgccgc atccgctggc aggccaacga ctatggcacc
      661 gtgcgctggc agctcatggt gatgcaggct tgcacgccgc tggacgtggc gcagcgcatc
      721 cctggcgtgc agccgggcgc gcgcctgctg ctacacgccg agggcgacgg gcaagttcgc
      781 gctgtgctgg aacgcattga cgccatcgag gcgctgggca tcgcgcccgc cgccgtctcg
      841 cccgcgtact ggcgcacgct gggcaaccgg ctcgccgcgc gcctgccgct gcccgaatac
      901 accgccgagc ggcacgccgc ctggctgacc gggagggcgc tgtcatgacg cttccatccg
      961 ctgctgccgg tgcaaccggc attccgccgc gtccttgctc gcgcctgtac gctcgcctcg
     1021 tgctcgcggg cctgtccgcc tgcggcctcg ctgcggtggc ttgggcgtcc ttcgtgcatc
     1081 cgttgccgcg tctgatctac aacccgtccg acagtgtggc ggtcggctgg tatcgcgtcg
     1141 atccgctggg ccacggtgcc ggctcgctgc cacgtccgtt gtccgttggc agcatcgtgc
     1201 tgaccacgct gccgccagag gctgccgcgc tggccgcgca gcgcggctac ctgccggcgc
     1261 gcgtgccgct gctcaagcgt gtgggtgcgg tagctccgca gactgtgtgc atcttcgatg
     1321 cgctggtctg gatcgacggc gtgccaatgg ccgccgtgct gcccgccgac cggctgggcc
     1381 gtccgctgcc agcgtggccg cagtgccgtc gccttgaacc cggcgaactg ttcctgctca
     1441 gtgagaccaa tccggcgtca ttcgacagcc ggtatttcgg gccggtcagc gcacccgccg
     1501 tgatcggcgt cgcgcatccg atctggctgg agtctcgccc atgatggccg ccgattcgct
     1561 gcacgttgcc gtgcatctca tcgtgccatc ggacgtgccg ctccggtggc tctccgcatg
     1621 tcgtcgcgcg tgcagtgcca gcgcatccgc gctgcacttc gcgctgcctt cccatgtgca
     1681 ggcgtcttgc ctcg
//
LOCUS       DAOMVO010000447         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_20950_length_1682_cov_9.785495, whole genome shotgun sequence.
ACCESSION   DAOMVO010000447 DAOMVO010000000
VERSION     DAOMVO010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20950_length_1682_cov_9.785495"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="pdxA"
                     /locus_tag="PKV30_13925"
     CDS             <1..840
                     /gene="pdxA"
                     /locus_tag="PKV30_13925"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRL31350.1"
                     /translation="VPVLQVVEPPAVPVPLGRISAVAGRMAGDAVDWAARAALRGDIA
                     GLVTAPLHKEALSAAGDPYARYPGHTELLQAVAAEHLGKPVAELPVRMMLANDELRTV
                     LVSIHLSLRDAIEKVTVGNILQTLRIAHTSVGAALQRQPRIAVAGLNPHAGEGGLFGR
                     EEIEAIAPAIAQARAEGIDAQGPFAPDTVFMRARGFAEFDVVVAMYHDQGLIPVKYLG
                     VEQGVNVTLGLPLVRTSPDHGTAFDLAGSGRADAASLLAAVRMARRMAQTKTPVAQGE
                     RAS"
     gene            complement(875..1288)
                     /gene="mscL"
                     /locus_tag="PKV30_13930"
     CDS             complement(875..1288)
                     /gene="mscL"
                     /locus_tag="PKV30_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRL31351.1"
                     /translation="MGMMQEFREFAVKGNAIDLAVGVIIGGAFGKIVDSIVGDLIMPI
                     VGRLFGGLDFSSYYIPLAGQASGLALEEAKKAGAVFAYGNFITILVNFILLAFIIFMM
                     VKQINRLKREEPAPAPEAPPEEVQLLREIRDSLKR"
     gene            1440..>1682
                     /locus_tag="PKV30_13935"
     CDS             1440..>1682
                     /locus_tag="PKV30_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HRL31352.1"
                     /translation="MSDAPTSKFTPAPMDSSKRTWLIASSCAGAAGAAALATPFVASF
                     APSERAKAAGAAVEVDIAAVKPGEKITVEWRGKPVWI"
BASE COUNT          267 a          569 c          572 g          274 t
ORIGIN      
        1 gtgccggtgc tgcaggtggt cgagccgccg gccgtgccgg tgccgctggg gcgcatcagc
       61 gccgtggccg gccgcatggc gggcgatgcc gtggactggg ccgcgcgcgc ggcgctgcgc
      121 ggcgacatcg cgggcctggt caccgcgccg ctgcacaagg aggcgctgtc ggccgccggc
      181 gacccgtatg cgcgctaccc cggccacacc gagctgctac aggctgtcgc cgctgaacac
      241 ctgggcaaac ccgtggccga gctgccggtg cgcatgatgc tggccaacga cgaactgcgc
      301 accgtgctgg tgagcattca cctgtctttg cgcgatgcta tcgaaaaggt aacagtgggc
      361 aacatcctgc agacgttgcg catcgcgcac acttctgttg gggcggcgct acagcggcag
      421 ccccgcatcg cggtggccgg gctcaacccg catgcgggcg aaggcgggtt gttcgggcgc
      481 gaggagatcg aggcgatcgc ccccgccatc gcccaggcgc gcgccgaagg catcgacgcc
      541 caaggcccct tcgcgcccga caccgtgttc atgcgcgcgc gcggcttcgc cgagttcgac
      601 gtggtggtgg cgatgtacca cgaccagggg ctgatcccgg tgaagtacct cggcgtggag
      661 cagggcgtga acgtcacgct gggcctgccc ctcgtgcgca ccagccccga ccacggcacc
      721 gccttcgacc tcgccggcag cggccgcgcc gacgcggcca gcctgctggc ggcggtgcgc
      781 atggcacgcc gcatggcgca aacaaaaacg cccgtcgcgc agggcgaacg ggcgtcttga
      841 gctacggttt gtgtagcgtt ttcgcttgat ctgatcagcg cttcaggctg tcgcggattt
      901 cgcgcagcag ctgcacctct tccggcggcg cctcgggggc gggggccggc tcttcgcgct
      961 tcaggcggtt gatctgcttg accatcatga agatgatgaa ggccagcagg atgaagttca
     1021 ccaggatggt gatgaagttg ccgtaggcga acacggcgcc ggccttcttg gcctcttcca
     1081 gcgccaggcc gctggcctgg cccgccagcg ggatgtagta gctggagaag tccagcccgc
     1141 cgaacagccg gccgacgatc ggcatgatca ggtcgcccac gatcgagtcg acgatcttgc
     1201 cgaaggcgcc gccgatgatc acgcccacgg ccagatcaat ggcgttgccc ttgacggcaa
     1261 attccctgaa ctcttgcatc attcccatgt tgtttccctc agcgggttgt tgaagacggc
     1321 gccagtattg cgaccgcttg atggttgtca atcccgtgcg ctcggcccgc cgggggcgat
     1381 gttgcgctgc gctacaatct cccgttgatc cggacacaat aaatcgccag aggacagcca
     1441 tgagtgacgc ccccacaagc aaattcaccc cagcgcccat ggacagcagc aagcgaacct
     1501 ggctcatcgc ctcgagttgc gctggcgctg ccggtgccgc ggccttggca acccccttcg
     1561 tcgccagctt cgcgccctcc gagcgggcca aggcggccgg tgccgccgtc gaggtggaca
     1621 tcgccgcggt gaagcccggt gagaagatca ccgtcgagtg gcgcggcaag ccggtgtgga
     1681 tc
//
LOCUS       DAOMVO010000448         1666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21227_length_1666_cov_12.399131, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000448 DAOMVO010000000
VERSION     DAOMVO010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21227_length_1666_cov_12.399131"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PKV30_13940"
     CDS             <1..1296
                     /locus_tag="PKV30_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HRL31353.1"
                     /translation="PGNARALVQAHGEGTTARDVWLNPPPIPLTTAEMDHIFDLPYAR
                     NPHPSYADARGRHDGETKIPAWEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSEA
                     SILREVEEMRDKVQGFTGVVSDLGGPTANMYRLGCKTPQIEAACRKPSCVYPGICQNL
                     TTDHAPLTRIYQRTRALPGVKKVLIGSGLRYDLAIRSPSYVKELVQHHVGGYLKIAPE
                     HTEEGPLTKMMKPGIGNYDKFKQLFEKFSAEAGKQQFLIPYFIAAHPGTTDEDMLNLA
                     LWLKKNGFRADQVQAFYPSPMATATTMYHTGLNPLVGVHRDERGEQVDTVKGERRRRL
                     HKAFLRYHDPNNWPLLREALKKMGRGELIGNGKQHLIPTWQPVTDGSYQSARRTNSSS
                     ASKPATTPELQKKKQRVASAPRRGAVLTQHTGLPPRKTR"
     gene            complement(1408..>1666)
                     /locus_tag="PKV30_13945"
     CDS             complement(1408..>1666)
                     /locus_tag="PKV30_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HRL31354.1"
                     /translation="MNNAATAAVMCPIALGAARQLDAQPQSCLMAVAIGASCAFLTPT
                     GHQNNTLILGPGGLKFGDYWRLGLSLDIVVVLVAVPMLLWM"
BASE COUNT          352 a          576 c          515 g          223 t
ORIGIN      
        1 cccggcaacg cccgcgcgct ggtgcaggcg cacggtgaag gcacgacggc gcgcgacgtg
       61 tggctgaacc cgccgcccat cccgctcacc acggccgaga tggaccacat cttcgacctg
      121 ccctacgcgc gcaacccgca ccccagctat gccgacgcgc gcgggcggca cgacggcgag
      181 acaaaaattc ccgcgtggga gatgatccgc ttctccgtca acatcatgcg cggctgcttc
      241 ggcggctgca ccttctgcag catcaccgag cacgaaggcc gcatcatcca gagccgcagc
      301 gaagccagca tcctgcgcga ggtggaagag atgcgcgaca aggtgcaagg gttcaccggc
      361 gtggtcagcg acttaggggg gccaacggcc aacatgtacc gcctgggctg caaaacgccc
      421 cagatcgagg ccgcctgccg caagcccagc tgcgtgtacc ccggcatctg ccagaacctg
      481 accaccgacc acgcgccgct cacgcgcatc taccagcgca cgcgcgcgct gccgggcgtc
      541 aagaaagtgc tgatcggctc cggcctgcgc tacgacctgg cgatccgctc gcccagctac
      601 gtgaaagagc tcgtgcagca ccacgtcggc ggctacctga agatcgcgcc cgagcacacc
      661 gaggaagggc cgctgacgaa gatgatgaag cccggcatcg gcaactacga caagttcaag
      721 cagctgttcg agaagttcag tgccgaggcg ggcaagcagc aattcttgat cccgtacttc
      781 atcgccgccc accccggcac cacggatgaa gacatgctga acctggcgct gtggctgaag
      841 aagaacggct ttcgcgccga ccaggtgcag gccttctacc cctcgcccat ggcgacagcg
      901 acgaccatgt accacaccgg cctgaacccg ctggtcggcg tgcaccgcga cgagcggggc
      961 gagcaagtgg acacggtgaa gggcgagcgc cgccgccgcc tgcacaaggc ttttttgcgc
     1021 taccacgacc ccaacaactg gccgctgctg cgcgaggcgc tgaaaaaaat gggccggggc
     1081 gaattgatcg gcaacggcaa gcagcacctg atcccgacct ggcagccggt cacggacggc
     1141 agctaccaga gcgcgaggcg caccaactcg tcgtccgcca gcaagcctgc gaccacacca
     1201 gagctgcaaa aaaagaagca gagagtcgcc tctgcacccc gacgtggcgc ggttttgacg
     1261 cagcacacgg gcttgccgcc gcgcaagact cgctaggtgc ttgaagggcg acctggcccg
     1321 ctcaccgcca ggctgccata cgaaaaacag gcgttcgatg cttgctccat atggatatgt
     1381 ggctatcaaa aggatagttc acagcggtca catccacagc agcatcggca ccgcaaccag
     1441 cacgaccacg atgtccagcg acaggccgag ccgccagtag tcgccaaact tcaaaccgcc
     1501 cgggcccagg atcagggtgt tgttctggtg cccggtcggc gtgagaaaag cgcacgaggc
     1561 accgatggcc accgccatca gacacgactg cggctgcgcg tccagctggc gcgctgcgcc
     1621 cagggcgatg gggcacatca cggccgcggt ggcggcgttg ttcatg
//
LOCUS       DAOMVO010000449         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21441_length_1653_cov_14.362328, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000449 DAOMVO010000000
VERSION     DAOMVO010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21441_length_1653_cov_14.362328"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKV30_13950"
     CDS             complement(<1..856)
                     /locus_tag="PKV30_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRL31355.1"
                     /translation="MFKKILIANRGEIACRVASTARRMGIKTVAVYSDADAHAKHVMA
                     CDEAVHVGGSAPKDSYLQWQRIIDAAKQTGAQAIHPGYGFLSENEDFAQACADAGLVF
                     IGPPASAINAMGLKAESKRLMEAAQVPLVPGYHGADQDPALLQKEADRIGYPVLIKAS
                     AGGGGKGMRAVDKAEDFIAALDSCKREATNSFGDDAVLVEKYVQRPRHIEIQVFGDTH
                     GNYVYLFERDCSVQRRHQKVLEEAPAPGMTESLRDAMGEAAVNAARAVNYVGAGTVEF
                     IVEQPGGYD"
     gene            complement(877..1638)
                     /locus_tag="PKV30_13955"
     CDS             complement(877..1638)
                     /locus_tag="PKV30_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRL31356.1"
                     /translation="MQELIQNIVQWLHGMGIHPNADTAAAVGHAVGQAGATVGDAVAP
                     VVAKMDMPSLMALAAALGWASGFRLYAVVFLVGAMGYAGWIPLPAGLHILEHPAVMAA
                     SGFMLLVEFFADKIPWIDSIWDTVNSVIRIPGGALLAAGVFGADSGTMGLVAGLLGGS
                     LAATSFATKATTRAAINTSPEPFTNWLASFFEDGLAVAVVWLATQYPVTFGVVLAVML
                     LVSVLLLVLLWKFLKMVIAKLKAFFSSGAPEPGRA"
BASE COUNT          274 a          566 c          519 g          294 t
ORIGIN      
        1 cgtcgtagcc gcccggctgc tcgacgatga actccaccgt gccggcgccc acgtaattga
       61 ccgcgcgcgc cgcgttgacc gcggcctcgc ccatcgcatc gcgcaggctt tcggtcatgc
      121 cgggcgcggg cgcttcttcc agcaccttct ggtggcggcg ctgcaccgaa cagtcgcgct
      181 cgaacaggta gacgtagttg ccatgcgtgt cgccaaacac ctggatttcg atatggcgcg
      241 ggcgctgcac gtatttttcg accagcacgg cgtcatcgcc aaagctgttg gtggcttcgc
      301 gcttgcaact gtccagcgcg gcgatgaaat cttccgcctt gtccaccgcg cgcatgccct
      361 tgccgccgcc gcccgcgctg gccttgatca gcaccgggta gccgatgcgg tcggcttctt
      421 tttgcagcag tgccggatct tgatcggcgc cgtggtagcc cggcaccagc ggcacctgtg
      481 ccgcttccat cagtcgtttg gattcggcct tcaaacccat cgcgttgatg gcgctggcgg
      541 gcggcccgat gaagacaagc cccgcgtcgg cacaggcctg cgcaaagtct tcgttctcac
      601 tcagaaaacc gtagccgggg tgaatggctt gcgcgccagt ctgcttggcg gcatcgatga
      661 tgcgctgcca ttgcaggtaa ctgtctttgg gcgcgctgcc gcccacgtgc accgcctcgt
      721 cgcacgccat cacgtgcttg gcgtgcgcgt cggcgtcgga atacaccgcc acggtcttga
      781 tgcccatgcg gcgcgcggtg cttgccacgc ggcaagcgat ttcgccacgg ttggcgatca
      841 ggattttctt gaacacgttc agtctcctcg atgagttcac gccctgccgg gctcgggcgc
      901 gccgctgctg aaaaacgctt tcagcttggc gatcaccatc ttcaaaaact tccacaacaa
      961 gaccagcagc agcaccgata ccaacagcat caccgccagc acgacgccga aggtgacggg
     1021 gtattgcgtc gccagccaga ctacggccac ggccaagccg tcctcgaaga acgaggccag
     1081 ccagttggtg aagggctcgg gcgatgtgtt gatggcggcg cgcgtggtgg ctttggtggc
     1141 aaacgatgtg gcggccagcg agccacccag caacccggcg accaggccca tcgttccgct
     1201 gtcggcgccg aacacgccgg cagccagcag cgcgccgccg gggatgcgga tgacgctgtt
     1261 gaccgtgtcc cagatgctgt cgatccacgg aatcttgtcg gcaaaaaact ccaccagcag
     1321 catgaagccg ctggccgcca tcacggccgg atgctccagg atgtgcaggc cggccggcag
     1381 cggaatccat cccgcgtagc ccattgcgcc gaccaggaac accaccgcgt acaagcggaa
     1441 cccgctggcc cagccgagcg cggcggccag cgccatcagc gacggcatgt ccatcttggc
     1501 gaccacgggc gccacggcat cgcccacggt cgcacccgcc tggcccaccg cgtggccgac
     1561 ggcggcggcc gtgtcggcgt tggggtggat gcccatgccg tgcagccatt gcacgatgtt
     1621 ttggatgagt tcctgcatgt cggtgccgcc ttg
//
LOCUS       DAOMVO010000450         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21530_length_1647_cov_20.941583, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000450 DAOMVO010000000
VERSION     DAOMVO010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21530_length_1647_cov_20.941583"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKV30_13960"
     CDS             <1..588
                     /locus_tag="PKV30_13960"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL31357.1"
                     /translation="NVQALCQQLGLQLTADLPFKSDESDAQIAALAQRLGSSNTQLVF
                     NHGGIGVYEKLIRQARQQGVKVQFSAVNSGATQLAANLGPLAQGMVVAQVVPSPWERK
                     TAIAREYQDDFKQRHPGKAFSYGSLEGYITAKALVAALRLAGPQPTRESLVTGIEKAG
                     QLELSGLRNSYRPGQHLGMQLVDLSLVNPQGRFVH"
     gene            complement(613..>1647)
                     /locus_tag="PKV30_13965"
     CDS             complement(613..>1647)
                     /locus_tag="PKV30_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HRL31358.1"
                     /translation="DVSLDIASGELVALLGPSGCGKTTLLRIIAGLESADAGSILFSG
                     ADTTDVHVRERQVGFVFQHYALFRHMTVAENVAFGLRVKPRRERLSDAQIKAKVHELL
                     GLVQLDWLADRYPSQLSGGQRQRIALARALAVEPKVLLLDEPFGALDAKVRKELRRWL
                     RRLHDELHVTSIFVTHDQEEALEVADRVVVMNKGRIEQVGTPQEVWEHPASPFVYGFL
                     GDVNLFRGRATGGQVQVGDWQIAAPEHAHALDAEAMAFVRPSDLTVAPYAAGATSGLA
                     ARLDRALLIGPLARLELTPHDNPSHILEAHLPANEFRQLGVQEGDSVRVSPRKARVFL
                     ETVDDPAAMI"
BASE COUNT          261 a          549 c          575 g          262 t
ORIGIN      
        1 aacgtgcagg ccctgtgcca gcagctgggc ttgcagttga cggctgatct gccgttcaaa
       61 tcggatgaga gcgatgcgca gatcgccgcg ctggcccagc gccttggcag ctccaacacg
      121 cagctggtgt tcaaccacgg cggcattggc gtgtatgaaa agctgatccg ccaggcgcgc
      181 cagcagggcg tgaaggtgca gttcagcgcc gtgaactcag gcgccaccca actggcggcc
      241 aacctgggcc cgctggcgca gggcatggtg gtggcgcaag tggtgcccag cccgtgggag
      301 cgcaagacgg ccatcgcccg cgagtaccag gacgacttca agcaacgcca tcccggcaaa
      361 gcgttttcct acggcagcct ggaaggctac atcacggcca aggcgctggt ggcggcgctg
      421 cggctggccg gcccgcagcc cacgcgcgag agcctggtga ccggcattga aaaggcgggg
      481 cagctggagc tttccggctt gcgcaacagc taccgacccg gccagcactt gggcatgcag
      541 ttggtggatc tgtcgctggt caacccgcag gggcgctttg tgcattgagc gctcggcctg
      601 tgcgctgggc gctcaaatca tcgccgccgg gtcgtctacc gtttccagaa acacgcgcgc
      661 cttgcgtggt gacacccgca cgctgtcgcc ttcttgcacg cccagttggc ggaattcgtt
      721 ggccggcagg tgcgcttcca gaatgtggct ggggttgtcg tgcggcgtca actccagccg
      781 cgccaggggg ccgatgagca gggcgcggtc gagccgggcg gccaggccgc tggtggcgcc
      841 ggcggcgtaa ggggccacgg tcagatcgct ggggcgcacg aaggccatgg cttcggcgtc
      901 cagcgcgtgg gcgtgctcgg gcgcggcaat ttgccagtcg cccacctgca cctgcccgcc
      961 cgtggcgcgg ccacggaaca ggttcacatc gcccagaaag ccatagacga aggggctggc
     1021 ggggtgttcc cacacctctt gcggggtgcc cacttgctcg atgcggccct tgttcatcac
     1081 caccacgcgg tcggccactt cgagcgcttc ttcctgatcg tgcgtgacga agatgctggt
     1141 gacgtgcagc tcgtcgtgca ggcggcgcag ccagcggcgc aactccttgc gcaccttggc
     1201 gtcgagcgcg ccaaagggct cgtcgagcag cagcaccttg ggctccaccg ccagcgcgcg
     1261 cgccaaggca atgcgctggc gctgcccgcc cgacagttgc gacgggtatc ggtcggccag
     1321 ccagtcgagc tgcaccaggc cgagcagctc gtgcaccttg gccttgatct gcgcatcgct
     1381 caggcgctcg cggcgcggct tgacgcgcag gccgaaggcg acgttctcgg ccaccgtcat
     1441 gtggcggaac agcgcgtagt gctgaaacac gaagcctacc tgccgctcgc gcacgtgcac
     1501 atcggtggtg tcggcgccgc tgaacaggat gctgcccgcg tcggccgatt ccaagcccgc
     1561 gatgatgcgc agcagcgtcg tcttgccgca gcccgagggg ccgagcagcg cgaccagctc
     1621 gcccgaggcg atgtcgaggc tgacgtc
//
LOCUS       DAOMVO010000451         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21543_length_1646_cov_19.872407, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000451 DAOMVO010000000
VERSION     DAOMVO010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21543_length_1646_cov_19.872407"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1646
                     /gene="ppsA"
                     /locus_tag="PKV30_13970"
     CDS             <1..>1646
                     /gene="ppsA"
                     /locus_tag="PKV30_13970"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRL31359.1"
                     /translation="VFASLYNDRAISYRVHKGFAHDVVALSAGVQRMVRSDLGAAGVM
                     FTIDTESGFDQVVFITSSYGLGETVVQGAVNPDEFYVHKPMLAARKRAVIRRTLGSKL
                     IQMVFASADEKAASGKLVKTIDVPTEQRNRYSLTDEDVEQLARYALIIEQHYGRPMDI
                     EWGKDGGDGQLYILQARPETVKSQQQGKTEQRYRLKSSGSVLAEGRAIGQKIGTGPVR
                     LVRDISQMDQVQPGDVLVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPA
                     VVGTGDATERLKDGTLVTVSCAEGDTGHIYDGLLETEVTEVQRGAMPPIATKIMMNVG
                     NPQLAFDFAQLPNEGVGLARLEFIINNNIGVHPKAILDYPQVDGDLKKAVESVARGHA
                     SPRAFYVDKVAEGVATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGF
                     RGAARYISDDFREAFAMECEALKRVRDDMGLTNVQVMVPFVRTLAQAERVTQLLADRG
                     LARGQNDLKVVMMCEVPSNAILADEFLKFFDGFSIGSNDLT"
BASE COUNT          328 a          520 c          552 g          246 t
ORIGIN      
        1 gtgttcgcca gcctgtacaa cgaccgcgcc atcagctacc gcgtgcacaa gggctttgcg
       61 cacgacgtgg tggcgctgtc ggccggcgtg cagcgcatgg tgcgctccga cctgggcgcg
      121 gcgggcgtga tgttcaccat cgataccgaa agtggcttcg atcaggtggt gttcatcacc
      181 agcagctacg gcctgggcga gacggtggtg caaggcgcgg tgaacccgga cgagttctac
      241 gtgcacaagc cgatgctggc ggcgcgcaaa cgcgcggtga tccggcgcac cctgggctcc
      301 aagctgatcc agatggtgtt tgcttcagcc gacgaaaaag ccgcgtcggg caagctggtg
      361 aaaaccatcg acgtgccgac cgagcagcgg aaccgctact cgctgaccga cgaggacgtg
      421 gagcaactgg cgcgctacgc cctcatcatc gagcagcact atggccgccc gatggacatc
      481 gaatggggca aggacggcgg cgacggccaa ctctacatcc tgcaggcgcg ccccgaaacc
      541 gtgaagagcc agcagcaagg caagaccgag cagcgctaca gactcaagag cagcggatcg
      601 gtgctggccg agggccgcgc aatcggccag aagatcggca ccggcccggt gcggctggtg
      661 cgcgacatca gccagatgga ccaggtgcaa ccgggcgacg tgctggtgac cgacatgacc
      721 gaccccaact gggagccggt gatgaagcgc gccagcgcca tcgtcaccaa ccgcggcggg
      781 cgcacctgcc atgcggccat catcgcgcgc gagttgggca taccggccgt ggtgggcacg
      841 ggcgacgcca ccgagcggct gaaggacggc acgctggtca ccgtgagctg cgccgaaggc
      901 gacaccggcc acatctacga cggcctgctg gagaccgaag tcaccgaggt gcagcgcggc
      961 gccatgccgc cgattgccac caagatcatg atgaacgtgg gcaacccgca gctggcgttt
     1021 gactttgcgc agttgcccaa cgaaggcgtg ggtctggcgc ggctggaatt catcatcaat
     1081 aacaacattg gcgtgcaccc caaagcgatc ctcgactatc cgcaggtcga tggcgatttg
     1141 aagaaagcgg tcgagagcgt ggcgcgcggc catgcgtcgc cgcgtgcctt ttacgtcgac
     1201 aaggtggccg aaggcgtggc caccatcgcg gccgcattct ggcccaagcc ggtcatcgtg
     1261 cgcctgtcgg acttcaagag caacgaatac cgcaagctga tcggcggcag ccgctacgag
     1321 ccggaagaag aaaaccccat gctgggcttt cgcggcgccg cgcgctacat cagcgacgat
     1381 ttccgcgagg cgtttgccat ggaatgcgaa gcgctcaagc gcgtgcgcga cgacatgggc
     1441 ctgaccaacg tgcaggtgat ggtgcccttc gtgcgcacgc tggcgcaggc cgagcgcgtg
     1501 acgcaattgc tggccgaccg tggcctggcg cgcggccaga acgacctcaa ggtcgtgatg
     1561 atgtgcgagg tgccgagcaa cgccatcctg gccgacgaat ttttgaagtt cttcgacggc
     1621 ttctccatcg gctccaacga cctgac
//
LOCUS       DAOMVO010000452         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21621_length_1642_cov_17.252678, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000452 DAOMVO010000000
VERSION     DAOMVO010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21621_length_1642_cov_17.252678"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKV30_13975"
     CDS             complement(<1..362)
                     /locus_tag="PKV30_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HRL31360.1"
                     /translation="MNLSLIRARQAQRGSTFLGIILGVVVGLAAALGVAIYVAKVPVP
                     FVNKNTSRTSGQDAAEAQKNRDWDPNAPLRSRNGAKPSTPSSGVATGTVAPAPDPAPI
                     PAPAEVTRATPRTPAVPAP"
     gene            complement(377..>1642)
                     /gene="argS"
                     /locus_tag="PKV30_13980"
     CDS             complement(377..>1642)
                     /gene="argS"
                     /locus_tag="PKV30_13980"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRL31361.1"
                     /translation="GHGRQAALGDAICNLFATQGWQVHREFYYNDAGVQIETLANSVQ
                     LRAKGLKPGDAGWPEAAYNGDYIADIAADFLAKKTVQADDRSFTASGDVEDLDSIRQF
                     AVAYLRHEQDKDLQAFRLKFDEYYLESSLYTSGRVEATVKKLIDAGKTYEQDGALWLR
                     STDYGDDKDRVMRKSDGGYTYFVPDVAYHISKWERGYPKVVNIQGTDHHGTIARVRAG
                     LQAAGVGIPAGYPDYVLHTMVRVVKGGEEVKISKRAGSYVTLRDLIEWTSADAVRFFL
                     LSRKPDTEYTFDVDLAVAKNNDNPVYYVQYAHARICSVLDAWGGDVATLAEADLAPLD
                     TSAAQALMLQLAKYPEMLTAAAEDFAPHDVTFYLRELAASYHSYYDAERILVDDETVK
                     KARLALVAATAQVLHNGLSVLGVSAPTRM"
BASE COUNT          238 a          527 c          574 g          303 t
ORIGIN      
        1 ggcgccggca ccgcgggcgt gcgcggcgtg gcgcgcgtga cttcggcggg cgcggggatc
       61 ggcgccggat cgggcgcggg cgccacggtg ccggtggcaa cgccgctgct gggcgtgctg
      121 ggcttggcgc cgttgcggct gcgcagcggg gcgttcgggt cccagtcgcg gtttttctgt
      181 gcctcggcgg cgtcctggcc gctggtgcgc gaggtgttct tgttgacgaa gggcaccggc
      241 accttggcga cgtagatcgc cacgcccagc gccgccgcca ggccgacgac cacgccgagg
      301 atgatgccga ggaaggtgct gccgcgctgc gcctggcgcg cacgaataag actcaaattc
      361 atggtgggat aacgctctac atccgggtgg gggccgacac gcccagcacc gacaggccat
      421 tgtgcaacac ctgcgccgtc gcggcgacca gggccagacg ggcttttttc accgtttcat
      481 catcgaccag gatgcgctcg gcgtcgtaat agctgtggta gctggcggcc agctcgcgca
      541 ggtagaaggt cacgtcgtgc ggggcaaagt cctctgcggc ggcggtgagc atctcggggt
      601 acttggccag ctgcagcatc aacgcctgcg ccgccgaggt gtcgagcggc gccagatcgg
      661 cctcggccaa cgttgccacg tcgccgcccc aggcgtccag caccgagcag atgcgggcat
      721 gtgcgtactg cacgtaatac accgggttgt cgttgttctt ggccaccgcc aggtcgacat
      781 cgaaggtgta ttcggtgtcg ggcttgcggc tgagcaaaaa gaaacgcacc gcatcggcgc
      841 tggtccattc gatcagatcg cgcagagtga cgtagctgcc ggcgcgcttg ctgatcttga
      901 cctcttcgcc gcccttcacc acgcgcacca tggtgtgcag cacgtagtcg gggtagccgg
      961 cggggatgcc gacgcccgcc gcctgcagcc cggcgcgcac gcgggcgatg gtgccgtggt
     1021 ggtcggtgcc ctggatgttc acgaccttgg ggtagccgcg ctcccacttg ctgatgtggt
     1081 aggccacgtc gggcacgaaa taggtgtagc cgccgtcgga cttgcgcatc acgcggtctt
     1141 tgtcgtcgcc gtagtcggtc gagcgcagcc acagggcgcc gtcctgctcg taggtcttgc
     1201 cagcgtcgat cagcttcttg accgtggctt cgacccggcc gctggtgtac aggctcgatt
     1261 caaggtagta ctcgtcgaac ttgaggcgaa acgcctgcaa gtccttgtcc tgctcgtggc
     1321 gcaggtaggc gacggcgaac tggcggatgc tgtccagatc ctccacatca ccgctggcgg
     1381 tgaagctgcg gtcgtccgcc tgcacggttt ttttcgccag aaagtcggcc gcgatgtccg
     1441 cgatgtagtc gccgttgtac gccgcttcgg gccagccggc gtcgccgggc ttcaagccct
     1501 tggcgcgcag ctgcaccgag ttggccagcg tctcgatctg cacgccggca tcgttgtaat
     1561 aaaactcgcg gtgcacttgc cagccttgcg tggcgaacag attgcagatg gcatcgccca
     1621 gcgccgcctg gcgcccgtgg cc
//
LOCUS       DAOMVO010000453         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21769_length_1634_cov_10.767574, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000453 DAOMVO010000000
VERSION     DAOMVO010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21769_length_1634_cov_10.767574"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..1325
                     /locus_tag="PKV30_13985"
     CDS             348..1325
                     /locus_tag="PKV30_13985"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRL31362.1"
                     /translation="MPTPLIPPNYDHVTGAANDTTAIDRLLVLAGGGAANAADSGSSA
                     QVNWSVADQQLCDTSRNASSDACVKRAMGQVMYTVLRFPQAGSYTLSLSHDDAGGLDL
                     ASDAGGPGYRDAPFQPVARLPRWTGQAAPETLTTYTTTQPNACVLARLTWNNWGTTNH
                     YGLYWSGPGIVGTALVPASALLDPSVTQAANCIMPIDAANDSAALAPGAPSVLVPVLA
                     NDTAGNGGTLDAASVAVVTPPAAGSATCTAAGCTYTPPAGGLTASVTFTYRVCLAAPN
                     QALCDVATVTIAPAAAGGVAAVPVGGREALAALSLLLGLAGIWQRRRRI"
BASE COUNT          258 a          563 c          574 g          239 t
ORIGIN      
        1 gccgcgcggg cgccgccggc gatcagcagc gcggcgctgg cgatcagggt gcggcggtgc
       61 agcagggtca tgggagactc ctttggagtg ttggggaaag gcgccggcgc cgacgtcggc
      121 tggcacggcg ggctgaaggt cactgtacgg aaatggcaac cgcgctggcg agcttgatac
      181 catagccagc atcgcgtacc gggtcgcaag taccccacag gtgcgcacag gggacaagac
      241 tcatgagacg agcacagttg aggtgggcaa ccgcggcgat gttgatgtgc atggccgctt
      301 cgtcgcaggc gagtgtgcta tgggaacggt atcgtggcca gcagtatgtg cccacgccac
      361 ttatcccgcc caattacgac cacgtgacgg gcgcggcaaa cgacaccacg gccatcgacc
      421 gattgctggt cttggctggt ggtggcgccg ccaatgcggc ggacagtggc agcagcgcgc
      481 aagtcaactg gtcggtggcc gatcagcaac tgtgcgacac cagtcgaaac gccagctcgg
      541 acgcgtgtgt caaacgcgcg atgggccagg tgatgtacac cgtgttgcgc tttccacagg
      601 ccggcagcta caccctgagc ctgagccacg acgatgccgg cggcctggat ctggccagcg
      661 atgcgggcgg cccgggctac cgagacgcgc cgtttcagcc cgtggcgcgc ctgccccggt
      721 ggaccggtca agccgcgcca gagacgctga ccacctacac cacgacccaa ccgaacgcat
      781 gcgtgctggc gcgcctgacg tggaacaact ggggcacgac caaccactac ggcctgtact
      841 ggtccggccc aggcattgtc ggcaccgcgc tggtgccggc gtctgccttg ctggacccat
      901 cggtcacgca ggccgccaac tgcatcatgc cgatcgacgc cgcgaacgac agcgcggcgc
      961 ttgcgccggg cgcgccttcg gtgctggtgc ccgtgctggc caacgacacc gccggcaacg
     1021 ggggcaccct cgacgccgcg tcggtggcgg tcgtcacgcc gccggctgca ggcagcgcca
     1081 cctgcactgc ggccggttgc acctatacgc caccggcggg cgggttgacg gcgtctgtca
     1141 ccttcaccta ccgcgtttgc ctggccgcgc ccaaccaggc gctgtgcgac gtcgccacgg
     1201 tcaccatcgc gccggccgct gccggcgggg tcgctgccgt gccggtgggt gggcgcgagg
     1261 cgctggcagc gctgtcgctg ctgctgggac tggcggggat ttggcagcgg cgccgccgca
     1321 tctgaggccc tggtcgtgcg accgcgctcg gcgcgcgact tgccagcccc gccgggcgtg
     1381 gctgggtgag cgcgagcgat ggcggcacat tgcacgagcc gaaggcgact tgcgttgtgt
     1441 catccaatgc ggtgaccgca agcgcgacaa tccagggatt tagcgcccgc gctgccccgc
     1501 actcgagtca ccatggacca gcactacctc acccccctgt tcgagccgca gtcgatcctg
     1561 ctcttcaccg gcccgcagag cgcgcccgag cagcagacgc cgcaggcgcg cgcgctgctg
     1621 gcgcacatgg cggc
//
LOCUS       DAOMVO010000454         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_21782_length_1633_cov_23.884030, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000454 DAOMVO010000000
VERSION     DAOMVO010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21782_length_1633_cov_23.884030"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKV30_13990"
     CDS             complement(<1..818)
                     /locus_tag="PKV30_13990"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HRL31363.1"
                     /translation="MLAPNADPIVAIATAPGRGAVGIVRVSGRDVRPLTRALTGRDLA
                     PRVATYGPLKDADGSPIDHGLALHFPAPHSYTGEDVLELQAHGGPVVLQLLLQRCLQA
                     AVEIDLATGAQRLPRLRLARPGEFTERAFLNNKLDLAQAEAVMDLIDASTESAARSAS
                     RSLSGEFSRQIHQLTDGLIHLRMLIEATLDFPEEEIDVLRSQDVRGQLSNLQQTLSGV
                     QARARQGALLREGIKVVIAGQPNAGKSSLLNALAGAELAIVTPIAGTTRDVVQQT"
     gene            850..>1633
                     /locus_tag="PKV30_13995"
     CDS             850..>1633
                     /locus_tag="PKV30_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRL31364.1"
                     /translation="MNATLFKNVHVLDVRAGQLLEDHQVLVEGDAIRELSDRPITATT
                     ARVIDGGGRTLMPGLIDCHVHVTFSQTNLGLLAQTPQTTLALRALPILRGMLRRGFTT
                     VRDAGGADWALKRAVDDGIDGTVTGPRLFVSGPALSPTGGHGDFRPRSDRLQPMGCGC
                     QRLASTRVVDGVDAVRGAVRELLQMGADQIKIMSSGGVASPTDPVSALGYSLEETRAI
                     VDEAAARHTYVLAHAYTPEAIRRAVECGVRSIEHGNLIDAPTA"
BASE COUNT          242 a          566 c          577 g          248 t
ORIGIN      
        1 gtctgctgca ccacgtcgcg cgtggtgccg gcaatggggg tgacgatggc cagctcggcc
       61 cccgccagcg cgttcaacag gctgcttttg ccggcgttgg gctggccggc gatgaccacc
      121 ttgatgccct cgcgcagcag cgcgccctgg cgtgcgcgcg cctgcacgcc ggacagtgtt
      181 tgctgcaagt tcgatagctg accgcgcacg tcctgactgc gcaggacgtc gatttcttct
      241 tcaggaaagt cgagcgtggc ctcgatcaac atgcgcaggt ggatcagacc atcggtgagt
      301 tggtgaatct ggcgcgagaa ctcgcccgac agcgagcggc tggccgagcg cgccgcgctt
      361 tcggtgctgg cgtcgatcaa gtccatcacc gcttcggcct gcgccaggtc gagcttgttg
      421 ttcaggaacg cgcgctcggt gaactcgccc ggccgcgcca ggcgcaggcg cggcagtctt
      481 tgggcgccgg tcgccagatc gatttcaacc gccgcctgca ggcagcgctg cagcagcaac
      541 tgcagcacca ccgggccacc gtgagcctgc agctccagca cgtcttcacc ggtgtaggaa
      601 tgcggcgccg gaaagtgcag tgccaagccg tggtcgatcg ggctgccatc ggcgtccttg
      661 agcggcccat acgtcgctac gcgcggcgcc agatcgcgcc cggtgagcgc gcgcgtcagc
      721 ggccgcacgt cgcgccccga cacgcgcacg atgcccaccg cgccgcggcc gggcgcggtg
      781 gcgatggcga cgatggggtc ggcgttgggc gctagcatgg gcggattgtc cacgcggaac
      841 ggcagcccca tgaatgcgac cttgttcaag aacgtccacg tgctcgacgt gcgcgccggg
      901 caactgctgg aagaccacca ggtgctggtc gagggcgatg cgatccgtga attgtcggat
      961 cgccccatca cggcgacgac cgcgcgcgtg atcgacggcg gcggccgcac gctgatgccg
     1021 gggctgatcg actgccacgt gcacgtcacc ttttcgcaga ccaacctggg cttgctggcg
     1081 caaacgccgc agaccacgct ggcgctgcgc gcgctgccga tcctgcgcgg catgctgcgg
     1141 cgcggcttca ccaccgtgcg cgatgcaggc ggcgcggact gggcgctcaa gcgggcggtt
     1201 gacgacggta tcgacggcac cgtgaccggc ccgcgcctgt tcgtcagcgg cccggcgctc
     1261 agccccacgg gtgggcatgg cgactttcgc ccgcgctcgg accgcctgca gcccatgggc
     1321 tgcggctgcc agcggctggc cagcacgcgc gtggtcgatg gcgtcgacgc cgtgcgtggc
     1381 gccgtgcgcg agctgctgca gatgggcgcc gaccagatca agatcatgtc gagcggcggc
     1441 gtggcgtcgc ccaccgaccc ggtgagcgcg ctcggctatt cgctggaaga aacgcgcgcc
     1501 atcgtcgatg aagccgccgc gcgccacacc tatgtgctgg cgcacgcgta cacgcccgaa
     1561 gcgattcgcc gcgcggtgga atgcggcgtg cgcagcatcg agcacggcaa cctgatcgac
     1621 gcgcccaccg ccg
//
LOCUS       DAOMVO010000455         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22227_length_1610_cov_18.677814, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000455 DAOMVO010000000
VERSION     DAOMVO010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22227_length_1610_cov_18.677814"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1112
                     /locus_tag="PKV30_14000"
     CDS             3..1112
                     /locus_tag="PKV30_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL31365.1"
                     /translation="MLPPLFPVFAQEFGLSFSQLGFLMTVFFIISGTGQALAGFLVDK
                     VGARPVLFASFVCFICACLAGYFAQGYWGLMLVAMFAGLGNAPFHPVNFSILNQRVST
                     YRLGYAFSAHGLSGNLGWALTPVFLLFFTAWQGWRSGYLAAAILYACILAVLVLHRGH
                     LKTEVVRRAHDAPKGSDVAFLRLPVVWWCFAFFLLSTMTLAVVQNFASPIMQAMHGVS
                     FEAATTMITAYMLCGAAGMFAGGFVAARMPAASDRVVAWSMGIGAVLIVVAGTGWLGG
                     FGSMAVLAATGFAVGIGGPSRDMMIKKATPKGATGRVYGTVYSGLDMGFALAPLIYGF
                     FMDRGHYGLTLMLAAVFLMLSVGAALGVGKRTAAA"
     gene            1342..>1610
                     /locus_tag="PKV30_14005"
     CDS             1342..>1610
                     /locus_tag="PKV30_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRL31366.1"
                     /translation="MTQRIAGHVLVECLLAQGVTHAFGVPGESYLAALDGFHQHRDRI
                     SFVICRQEGGAAFMAEAHGKLTRRPGVCFVTRGPGATNASIGVHTA"
BASE COUNT          199 a          508 c          587 g          316 t
ORIGIN      
        1 tgctgctgcc gccgctgttt ccggtgttcg cgcaggagtt cgggctgtcg ttctcgcagc
       61 tcggctttct gatgacggtg ttcttcatca tttcgggcac cgggcaggcg ctggcgggtt
      121 ttctggtcga caaggtgggc gcgcggccgg tgctgttcgc atccttcgtg tgcttcatct
      181 gtgcctgctt ggcgggctac ttcgcgcagg gttactgggg actgatgctg gtggccatgt
      241 tcgccgggct gggcaatgcg ccgttccacc cggtgaattt ctcgatcctg aaccagcgcg
      301 tgtccacgta ccggctgggc tacgccttca gcgcgcatgg cctgtcgggt aacctgggct
      361 gggcgctgac gccggtgttc ctgctgttct tcaccgcctg gcagggctgg cgctcgggct
      421 atctggcggc ggccatcctg tacgcctgca tcctggcggt gctggtactg caccgcgggc
      481 acctgaagac cgaggtggtg cggcgcgcgc acgacgcgcc caagggcagc gacgtggcct
      541 ttctgcggct gccggtggtg tggtggtgct ttgcgttctt cctgctgtcg accatgacgc
      601 tggcggtggt gcagaacttc gcctcgccca tcatgcaggc catgcacggc gtcagcttcg
      661 aggcggcgac gaccatgatc accgcctaca tgctgtgcgg cgcggccggc atgttcgccg
      721 gcggcttcgt ggcggcgcgc atgccggcgg ccagcgaccg cgtggtggcc tggtcgatgg
      781 gcatcggggc ggtgctgatc gtggtcgccg gcaccggctg gctgggcggc ttcggctcca
      841 tggcggtgct ggcggccacc ggctttgcgg tcggcattgg cgggccgagc cgcgacatga
      901 tgatcaagaa ggccacgccc aagggcgcca ccgggcgcgt gtacggcacc gtctattcgg
      961 gcctggacat gggctttgcc ctggcgccgc tgatctacgg cttcttcatg gaccgcggcc
     1021 actacggcct gacgctgatg ttggcggcgg tgttcctgat gttgtcggtg ggggcggcgc
     1081 tgggggtggg caagcgcacg gcggcggcgt gacgaatgct ttcaaaaaca tagctgctcg
     1141 cgcttgacag ataagcactg gcggccgatt tgacctgaaa tcctgtggga gcggccttgg
     1201 ccgcaagacg cccggccgcg gagcaggcca tgccagcgac cgccgcggaa gggcttggcc
     1261 cgcccgctgg cggtgtcccc cctcgcacag cggagggggg aagcggcgtc agccgctcag
     1321 gggggtggtc ataatgtttc catgacccaa cgcatcgccg gccatgttct tgtcgaatgc
     1381 cttctggccc agggcgtcac ccatgccttc ggcgtgccgg gcgaaagcta cctggcggcg
     1441 ctggacggct tccatcagca ccgcgaccgg atttcattcg tcatctgccg gcaggagggc
     1501 ggcgccgcct tcatggctga ggcgcacggc aagctgacca ggcggccggg cgtgtgcttc
     1561 gtcacccgcg ggccgggggc gaccaatgcc agcatcggcg tgcacacggc
//
LOCUS       DAOMVO010000456         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22285_length_1607_cov_16.194588, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000456 DAOMVO010000000
VERSION     DAOMVO010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22285_length_1607_cov_16.194588"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1176)
                     /locus_tag="PKV30_14010"
     CDS             complement(19..1176)
                     /locus_tag="PKV30_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRL31367.1"
                     /translation="MHIVEFTAGSLRFVDAAPERAPDDGFVWIYLDRTDFVRHLADVQ
                     AAAQRLGGSALLDVHVKDLASDSHPSHYDATSVYDMVIFRRLATLAEVQRDQEQVNAI
                     ASYHAHGVGASALTKPLVPEAFSRIDSRAVGFAIFDKLLISVHPAGCYTANSYVQRFV
                     ADAKLAVDTAGTRNRVPQGPSDLVLRTINTMVDSYLDLRKDLATALENAQAELLKPDP
                     RQDTWSAMMHARRRLHALQDLCEEQQDAMQEWLDTLRELPLTAYHADPAQAQARRDQL
                     VARARDVMEHIDRVLHHARRLEQSAETAVQIHFSAQGHRTNDIMRTLTAVTAIFLPLN
                     LFTGFFGMNFEHLPLIHSTEGMWVALALLALLAGGILAIFWRRRYLTRTGR"
     gene            complement(1366..>1607)
                     /locus_tag="PKV30_14015"
     CDS             complement(1366..>1607)
                     /locus_tag="PKV30_14015"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592903.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRL31368.1"
                     /translation="MVKPGMPYLDIVRRVKDEFRVPTFAYQVSGEYAMLKAAAQNGWL
                     DHDAVMMESLLAFKRTGADGVLTYFARDAARLLAR"
BASE COUNT          249 a          502 c          573 g          283 t
ORIGIN      
        1 ttttgggcga tggattcgtc agcgccccgt gcgcgtcaaa tagcgccggc gccagaagat
       61 ggccaggatg ccgccggcca gcagcgcgag cagggccagg gcgacccaca tgccttcggt
      121 gctgtggatc agcggcaggt gctcgaaatt catgccgaaa aagccggtga acaggttcag
      181 cggaagaaag atcgccgtca ccgccgtcag cgtgcgcatg atgtcgttgg tgcggtggcc
      241 ttgcgcgctg aagtggatct gcaccgccgt ttcggccgat tgctcgagcc gccgcgcgtg
      301 gtgcagcacg cggtcgatgt gttccatgac gtcgcgcgcg cgcgccacca gctggtcgcg
      361 ccgcgcctgc gcctgcgccg ggtcggcgtg gtaggcggtc agcggcagct cgcgcagggt
      421 gtcaagccat tcctgcatcg catcctgctg ttcttcgcac aagtcctgca gcgcgtgcag
      481 gcggcggcgc gcgtgcatca tggcgctcca ggtgtcctgg cgcgggtcgg gcttgagcag
      541 ttcggcctgc gcgttctcca gcgcggtggc caggtccttg cgcagatcga ggtagctgtc
      601 gaccatggtg ttgatggtgc gcagcaccag gtcgctcggg ccctgcggca cgcggttgcg
      661 ggtgccggcg gtgtcgacgg cgagcttggc gtcggccacg aagcgctgca cgtagctgtt
      721 ggcggtgtag cagccggccg ggtgcacgct gatgagcagc ttgtcgaaga tggcaaagcc
      781 gacggcgcgc gaatcgatgc gcgaaaacgc ctcgggcacc agcggcttgg tcagcgccga
      841 cgcgccgaca ccatgcgcgt ggtaactggc gatcgcgttg acctgttcct ggtcgcgctg
      901 cacctcggcc agcgtggcca ggcgccggaa gatcaccatg tcgtagacgg atgtcgcgtc
      961 gtagtgcgag gggtgcgaat cgctcgccag gtccttcacg tgcacgtcga gcagcgccga
     1021 gccgcccagg cgctgcgcgg cggcctgcac gtcggccagg tggcgcacga agtcggtgcg
     1081 gtcgaggtag atccagacaa agccgtcgtc cggcgcgcgc tcgggcgccg catcgacaaa
     1141 gcgcaggctg ccggcggtga attcgacgat gtgcacgggc ggagggcttt atgggttaaa
     1201 tcggccgctg gcgcttgatg gacaagcgcg gtcagctatc atttttgatg aagcgcccac
     1261 ggcgcatgac ctcggccctt cggccatcga tgacggcgct gcgcgccatg acgggggtcc
     1321 aagtcatttc gtcattcagc gcagcgaagt catttcgtca ttccctcatc gcgccagcag
     1381 ccgcgccgca tcgcgcgcga agtaggtcag cacgccatcg gcgccggtgc gcttgaaggc
     1441 gagcaggctt tccatcatca ccgcgtcgtg gtcgagccag ccgttttgcg cggcggcctt
     1501 cagcatggcg tattcgccgc tgacctggta ggcgaaggtg ggaacgcgaa attcgtcctt
     1561 cacgcgccgc acgatgtcca ggtagggcat gccgggcttg accatca
//
LOCUS       DAOMVO010000457         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22415_length_1601_cov_16.614489, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000457 DAOMVO010000000
VERSION     DAOMVO010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22415_length_1601_cov_16.614489"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1182)
                     /locus_tag="PKV30_14020"
     CDS             complement(79..1182)
                     /locus_tag="PKV30_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRL31369.1"
                     /translation="MISIPSAFAPSYAQARVKFLEAAATASTAIRSFKHPLPGRDGEQ
                     LAMDVALDGDPASERLLIVSSACHGVEGYCGSGVQVFALHDDEWRAKARDAGVAVLYI
                     HALNPYGFSHIRRVTQENVDLNRNFHDFAQPLPVNTAYREIAELVLPATWPPDAANQQ
                     QMRQFVAERGLPVWQAAVSGGQHEYPDGLFYGGQAPTWSNQTLRQLLAAYAARAQDVA
                     WIDLHTGLGPSGVGERIWAGGHDDAAIARARRWWGDQVTSIYDGSSTSALLTGLMWGA
                     IHDAAPQAEYTGIALEYGTEPIMEVLDALRGDQWLAQHPEAPPELAASIKARLLKAFY
                     TDTDAWKGQVVSQARQAMFQAVDGLRADSAPAA"
     gene            complement(1227..1421)
                     /locus_tag="PKV30_14025"
     CDS             complement(1227..1421)
                     /locus_tag="PKV30_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31370.1"
                     /translation="MAQQPDEAAFPSAASLDADIVRCIWGYENGGSSQGRKSFFKRLV
                     WLEAQREQLFGLAAPRRAFR"
BASE COUNT          249 a          514 c          561 g          277 t
ORIGIN      
        1 gccgcgcgga gatgctcctg attcaatagc tgctggcgct tgtctggcaa gcgctggcgg
       61 ccgattcgat cctgaaaatc aggccgctgg ggcgctgtcc gcccgcaagc catccaccgc
      121 ctgaaacatc gcctgccgtg cctggctgac gacttggccc ttccaggcgt cggtgtcggt
      181 gtagaaagct ttcagaaggc gcgccttgat cgaggcagcc agttcgggcg gcgcctcggg
      241 gtgctgggcc agccactggt cgccgcgcag cgcatccagc acttccatga tcggctcggt
      301 gccgtattcg agcgcgatgc cggtgtattc agcctgcggc gcggcgtcgt ggatggcgcc
      361 ccacatcagg ccggtgagca gcgcgctggt cgatgagccg tcgtagatcg aggtcacttg
      421 atcgccccac cagcggcgcg cgcgggcgat ggcggcgtcg tcgtggccac cggcccagat
      481 gcgctcgccc acgccgctgg gccccaggcc ggtgtgcagg tcgatccagg ccacgtcttg
      541 cgcgcgcgcg gcgtatgcgg ccagcagctg gcgcagcgtc tggttgctcc aggtgggcgc
      601 ctggccgccg tagaacaggc cgtccgggta ttcgtgctgg ccgcccgaga cggcggcctg
      661 ccacaccggc aacccgcgct cggccacgaa ctgccgcatc tgctgctggt tggccgcatc
      721 aggcggccat gtggcgggca gcaccagctc ggcgatctcg cggtaggcgg tgttgacggg
      781 cagcggctgc gcaaagtcgt gaaagttgcg gttcaggtcg acgttctctt gcgtcacacg
      841 gcggatgtgt gagaagccgt aggggttgag cgcgtggatg tagagcaccg ccacgcccgc
      901 atcgcgcgcc ttggcgcgcc actcatcgtc gtgcagggcg aacacctgca cgccgctgcc
      961 gcaatagccc tcgacgccgt gacaggcgct gctgacgatg agcaggcgct cacttgcagg
     1021 gtcgccatcc agcgccacat ccatcgccaa ctgttcgccg tcgcggccgg gcagcgggtg
     1081 cttgaacgag cggatggcgg tgctggcagt ggcggcggct tcgaggaact tgacgcgggc
     1141 ctgggcgtag ctgggggcaa aagcggaagg gatggagatc atgagcgatg agcattggtg
     1201 ccacgccatg aagcgtgggc aatggtttat cgaaacgcgc ggcgcggcgc cgcaaggcca
     1261 aatagctgtt cgcgctgcgc ttcaagccaa accaggcgct tgaaaaatga ttttctgccc
     1321 tgagacgacc cgccgttctc gtaaccccag atgcagcgga cgatatcggc atcaagcgac
     1381 gccgctgacg gaaatgctgc ctcgtccggc tgctgtgcca taccggatcc ggggcgccgc
     1441 ttttttgtca cgtcactttt ggggcatcgg ccgatccgtc ggcggctcgt gccccagcca
     1501 cgcctcggcc agatgcaccc aatacgtgcc gcccagcggg atcagatcgt cgttgaagtc
     1561 gtagtgcggg ttgtgcagcg tgcaggggcc ggcgtcgtgg c
//
LOCUS       DAOMVO010000458         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22435_length_1600_cov_11.555340, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000458 DAOMVO010000000
VERSION     DAOMVO010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22435_length_1600_cov_11.555340"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PKV30_14030"
     CDS             <1..1475
                     /locus_tag="PKV30_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRL31371.1"
                     /translation="FVTRGPGATNASIGVHTASQDSTPMVLFVGDVASDQRDREAFQE
                     VDYRAFFGAFGMAKLVERIDSVERIPEYVARAFATAQNGRPGPVVLALPEDMLTQAVP
                     DGLQPMPRWQPPSYQPDPQSMQRLRELLLNAKRPIVIAGGGGWTPEGARDLQAFAESW
                     HLPVGNAMRFQDTFDNRHPLYAGDVGIAINPKLGQRIKDADLILAIGPRLGEMTTSGY
                     TLLKVPKPTQKLIHVHTSAEELGRVYQPDLALCASLPATAAALAALAPPDDRPWRDWA
                     DAVHQDHVANMQPQPLPGPIDMHAIVCTLQKLLPEDAAITNGAGNFASWTHRYFHFPG
                     IAKGHKTQLAPTSGAMGYGVPAGVACAITTGRTVVTVAGDGDFLMNGQELATARQYGA
                     KTIVVLLNNGVYGTIRMHQARDYPHRQEGTELVNPDFVRLAESYGYAGTRVERTEQFE
                     PALREALAREQGTVIEVMLSEQVITTRTTLDAIENASKKN"
BASE COUNT          296 a          545 c          520 g          239 t
ORIGIN      
        1 gcttcgtcac ccgcgggccg ggggcgacca atgccagcat cggcgtgcac acggcgtcgc
       61 aggattcgac gccaatggtg ctgttcgtcg gcgacgtggc cagcgaccag cgcgaccgcg
      121 aggcgtttca ggaagtggac taccgcgcct tctttggcgc cttcggcatg gccaagctgg
      181 tcgagcgcat cgacagcgtc gagcggattc ccgaatacgt ggcgcgcgcc tttgccacgg
      241 cgcagaacgg ccgacccggc ccggtggtgc tggcgctgcc cgaagacatg ctgacgcaag
      301 ccgtgccgga cggcctgcag ccgatgccgc gctggcagcc gccttcgtat caacccgacc
      361 cgcagagcat gcaacgcctg cgcgagctgc tattgaatgc gaagcgcccg atcgtcatag
      421 cgggcggtgg cggctggacg cccgagggcg cgcgcgatct gcaggcgttt gctgaaagct
      481 ggcacctgcc cgtcggcaac gcgatgcgtt ttcaggacac cttcgacaac cgccacccgc
      541 tgtacgctgg cgatgtcggc atcgccatca accccaagct cggccagcgc atcaaggatg
      601 ccgacctgat cctcgccatc ggcccgcgcc tgggcgagat gacgaccagt ggctacacgc
      661 tgctgaaagt gcccaagccg acgcaaaagc tgatccacgt gcacaccagc gccgaagaac
      721 tgggccgtgt gtaccaaccc gatctggccc tgtgcgccag cctgccggcc acggcggcgg
      781 cgctggcggc gctggcgccg ccggatgatc ggccgtggcg cgactgggcc gacgccgtgc
      841 accaggacca cgtggccaac atgcagccgc agcccttgcc cggcccgatc gacatgcacg
      901 ccatcgtctg cacgctgcag aaactgctgc ccgaggatgc cgccatcacc aacggcgccg
      961 gcaactttgc cagctggacg caccgctact ttcactttcc gggcatcgcc aagggccaca
     1021 agacacaact ggcgcccacc agtggcgcca tgggctacgg cgtgccggcg ggggtggcct
     1081 gcgccatcac caccggccgc accgtggtca ccgtggcggg cgatggcgac tttctgatga
     1141 acgggcaaga gctggccacg gcgcgccagt acggcgccaa gaccatcgtc gtgctgctca
     1201 acaacggcgt gtacggcacc attcgcatgc accaggcgcg tgactaccct cacaggcagg
     1261 aaggcaccga gctggtcaac cccgatttcg tgcggctggc cgaaagctat ggctacgcgg
     1321 gcacgcgggt cgagcgcacc gagcaattcg agccggcctt gcgtgaggcg ctggccagag
     1381 agcagggcac ggtgatcgag gtgatgctga gcgagcaggt catcaccacg cgcaccacgc
     1441 tggatgcgat cgagaacgcg tcgaagaaaa actgaagctg ctttgttttt gatagctgct
     1501 cgcgcttgcc aggcaagcgc cagcggctga ttcaaataaa aaaagccggc tcatcgagcc
     1561 ggcttttctt ttgcgcaacg cgcaggctgg gatcagccca
//
LOCUS       DAOMVO010000459         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22501_length_1597_cov_15.472114, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000459 DAOMVO010000000
VERSION     DAOMVO010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22501_length_1597_cov_15.472114"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKV30_14035"
     CDS             <1..537
                     /locus_tag="PKV30_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRL31372.1"
                     /translation="GLIKPGHRVVDLGSTPGAWSQYLRRRLSPGGAAVGELNGTIVAL
                     DILPMEAVEGVTFLLGDFREDAVLAQLDEAVGGQPLDVVVSDMAPNLSGIESSDSARI
                     AHLVELAVDFATRHLKPEGALVVKLFHGSGYSQLVKLFKQHFRVVKPIKPKASRDKSS
                     ETFLVGIGLKGVSGTLET"
     gene            721..>1597
                     /locus_tag="PKV30_14040"
     CDS             721..>1597
                     /locus_tag="PKV30_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRL31373.1"
                     /translation="MKNQWFSKIAVWMVIAMVLFTVFKQFDGGRMASASHMAYSDFLE
                     EVRNNRVKEATIQEGQGGTDIIAKMNDDRRVRTQATYLDRGLVGDLINHGVKFDVKPR
                     EEGSLLMTLLVSWGPMLLLIGVWVYFMRQMQGGGKGGAFSFGKSKARMLDENNNTVTF
                     ADVAGCDEAKEEVKEVVDFLKDPQKFQKLGGRIPRGLLLVGPPGTGKTLLAKSIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFENAKKNAPCIIFIDEIDAVGRQRGAGLGGG
                     NDEREQTLNQMLVEMD"
BASE COUNT          297 a          475 c          531 g          294 t
ORIGIN      
        1 ggcctgatca agcccggcca ccgggtggtg gatttgggca gcacgcccgg cgcctggagc
       61 cagtacctgc gccgccgcct gtcgcccggc ggcgcggcgg tgggcgagct gaacggcacc
      121 atcgtcgcgc tcgacatcct gccgatggag gcggtggaag gcgtcacctt tctgctgggc
      181 gatttccgcg aggacgcggt gctggcgcaa ctcgacgagg cggtgggcgg ccagccgctg
      241 gacgtggtgg tgtcggacat ggcgcccaac ctgtcgggca tcgaatcgag cgattcggcg
      301 cgcatcgcgc acctggtgga gctggcggtg gatttcgcca cccgccacct caagcccgag
      361 ggcgcgctgg tggtcaagct gtttcacggc agcggctaca gccagctggt caagctgttc
      421 aagcagcact ttcgcgtggt caagccgatc aagcccaagg cgtcgcgcga caaatcgtct
      481 gaaacctttc tggtcggcat cggtctgaag ggcgtcagcg ggacgctgga gacttgacgc
      541 gcgtcaaggt tgcaaccgcc tgtatccgcc gtgctgccga ttcggtgcgc ttttcggcac
      601 taatacgacg ttcaccggca ggggctcatt gttcatccca cacgccgcag cggttgaaat
      661 gcctaaaatg gcctgcatat acccggcatt gccgggtttt tttgactgga gcgtaaggcc
      721 ttgaaaaatc aatggttttc gaagatcgcc gtctggatgg tgatcgccat ggtgctgttc
      781 actgtgttca agcagtttga cggcggccgc atggccagcg ccagtcacat ggcttattcc
      841 gattttctgg aagaggtgcg caacaaccgc gtgaaggaag ccaccatcca ggaaggccag
      901 ggcggcaccg acatcatcgc caagatgaac gacgaccgcc gtgtgcgcac gcaagccacg
      961 taccttgatc gtggcttggt cggcgacctc atcaaccatg gcgtcaagtt cgacgtcaag
     1021 ccgcgcgaag aaggctcgct gctgatgacg ctgctcgtca gctggggtcc catgctgctt
     1081 ttgatcggcg tgtgggtgta cttcatgcgc cagatgcagg gcggcggcaa gggcggcgcg
     1141 ttcagcttcg gcaagtccaa ggcgcgcatg ctggacgaga acaacaacac cgtcaccttt
     1201 gccgatgtcg ccggttgcga cgaggccaag gaagaggtga aggaagtcgt cgacttcctg
     1261 aaagacccgc aaaaattcca gaagcttggc ggccgcattc cgcgcggcct gctgctggtc
     1321 ggcccgcccg gcacgggtaa aacgctgttg gcgaaatcca tcgcgggcga agccaaggtg
     1381 ccgttcttca gcatctcggg ctccgacttt gtcgaaatgt tcgtcggcgt gggcgcggcg
     1441 cgcgtgcgcg acatgttcga gaacgccaag aagaatgcgc cttgcatcat cttcatcgac
     1501 gaaatcgacg ccgtgggccg ccagcgtggc gcgggcctcg gcggcggcaa cgacgagcgc
     1561 gagcagacgc tgaaccagat gctggtcgag atggacg
//
LOCUS       DAOMVO010000460         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22616_length_1591_cov_14.508464, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000460 DAOMVO010000000
VERSION     DAOMVO010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22616_length_1591_cov_14.508464"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..904)
                     /locus_tag="PKV30_14045"
     CDS             complement(110..904)
                     /locus_tag="PKV30_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRL31374.1"
                     /translation="MTDTLPMPVDVRLMNVTTALLVTGLVLACVVAGLWWVLRNPAFA
                     IRQIVVVGDTAHNSATSLRAAVAPRLAGNFFTLDLSSAQSAFQSAPWVRQAVVQREFP
                     DRLNVKLQEHVAVAHWGDDDTHLVNSFGEVFEASGGAELDERDLPVLIGPEGQATQLL
                     EMHRLLNPLVAPLDTKVAQLALQPRGNWQAELDSGAVIELGHGMPKDLAARLTQFVGT
                     VKEVAARHQRGVDAVEAADLRHAGGYALRLRGVSTVRPDAQPAVKR"
     gene            complement(901..>1591)
                     /locus_tag="PKV30_14050"
     CDS             complement(901..>1591)
                     /locus_tag="PKV30_14050"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRL31375.1"
                     /translation="SSMAIDKVMTKRVWLAEGLPTPRYALLHRGQFTREDVRTIPDDL
                     GLPLIVKPVREGSSLGVTKVMGYSQMQDAVAAAAALDADILCEQFIAGDEVTCPVLGE
                     GASARALPVIHIVAPEGNYDFQNKYYTDVTEYRVPCDLPPGEEVAIQAMVRKAYRVLG
                     CRGWGRLDVMIDGATRQPYLLEINTSPGMTSHSLVPMAAKAAGIDYETLCLQLLAGAS
                     LDYAPAPQACA"
BASE COUNT          244 a          554 c          517 g          276 t
ORIGIN      
        1 cctcgcccac caccaccatg atcttggcgg tgccgatgtc gaggccgacg atcaagtcct
       61 tgtactcttt tgccatgagg ttaccagtgc ctatgctctc ttttttatat caacgtttga
      121 ctgctggctg cgcgtcaggg cgcaccgtgc tgacaccgcg caggcgcagc gcatagccgc
      181 ccgcgtgccg caggtcggcc gcctcgacgg cgtccacgcc gcgctgatgg cgcgccgcca
      241 cttccttcac cgttcccacg aattgcgtca gccgcgccgc caggtccttc ggcatgccgt
      301 ggcccagttc gatgacggcc ccgctgtcca actcggcctg ccagttgccg cgcggctgaa
      361 gcgccagttg cgcgaccttg gtgtcgagcg gcgccaccag cggattcagc aggcggtgca
      421 tttccagcag ttgtgtcgcc tgaccctcgg gcccgatcag caccggcaga tcgcgctcgt
      481 ccagttcggc gccgccgctg gcctcgaaca cttcaccgaa actgttgacc agatgggtgt
      541 cgtcgtcccc ccagtgcgcc acggccacgt gctcttgcag cttcacgttg aggcgatcgg
      601 gaaactcgcg ctgcaccacc gcctggcgca cccagggcgc cgactggaaa gccgattgcg
      661 cgctcgacaa atcgagcgtg aagaagttgc ccgccagccg cggcgcgacg gccgcgcgca
      721 ggctggtggc gctgttgtgc gcggtgtcgc ccaccaccac gatctggcgg atggcgaacg
      781 ccgggttgcg cagcacccac cacagcccgg ccaccacgca ggccagcacc agcccggtga
      841 cgagcagcgc cgtcgtcacg ttcatcagcc ggacgtcgac gggcatgggc aaggtgtcgg
      901 tcatgcgcat gcttgcggcg ctggcgcgta gtcgagcgat gcgccggcca gcagttgcag
      961 gcacagcgtt tcgtagtcaa tgcccgccgc cttggcggcc atgggcacca gcgaatggct
     1021 ggtcatgcca ggcgaggtgt tgatttccag caggtagggc tggcgcgtgg cgccgtcgat
     1081 catcacgtcc agccggcccc agccgcggca gcccagcacg cggtaggcct tccgcaccat
     1141 ggcctggatg gccacctctt cacccggcgg caggtcgcag ggcacgcggt attcggtgac
     1201 gtcggtgtag tacttgttct ggaaatcgta attgccttcg ggcgccacga tgtggatcac
     1261 cggcagggcg cgcgcgctgg cgccttcgcc cagcaccggg caggtcactt cgtcgcctgc
     1321 gatgaactgc tcgcacagga tgtcggcatc gagcgcggct gcggcggcca ccgcatcctg
     1381 catctgcgaa tagcccatga ccttggtcac gcccagcgac gagccttcgc gcaccggctt
     1441 gacgatgagc ggcaggccca ggtcgtcggg aatggtgcgc acgtcttcgc gcgtgaactg
     1501 gccgcgatgc aagagcgcgt agcgcggcgt cggcaagcct tcggccagcc acacgcgctt
     1561 ggtcatcacc ttgtcgatgg ccatgctgga g
//
LOCUS       DAOMVO010000461         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22839_length_1577_cov_16.904074, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000461 DAOMVO010000000
VERSION     DAOMVO010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22839_length_1577_cov_16.904074"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PKV30_14055"
     CDS             complement(<1..553)
                     /locus_tag="PKV30_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRL31376.1"
                     /translation="MNAPLPEHIRKALETVTLDDKYSLDYGRAFMSGVQALVKLPMLQ
                     RQRDALIGKNTAGFVSGYRGSPLGGYDQALWGARKHLEAQHIVFQPGVNEELAATAIW
                     GTQQLGFAPPGSNKFDGVFGIWYGKGPGVDRTADVFKHANMAGTTPWGGVIAVAGDDH
                     ISKSSTAAHQSDHIFKACGLPVFF"
     gene            728..1213
                     /locus_tag="PKV30_14060"
     CDS             728..1213
                     /locus_tag="PKV30_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL31377.1"
                     /translation="METLDSFDIAILQQLQADGRMTNAELAQRVGLSAAPCWRRVREL
                     ERNGFITGYRAVIDRHKIGLGVLAFVRLDAAQSSGPQVQKMEAAIRAVPDIVSCHYIS
                     GSGTFELQVVSPDLDSFSRFAREVLLHLPNVKDMHTSFSLGEVKAAGALPLGHIARRP
                     R"
     gene            1307..>1577
                     /locus_tag="PKV30_14065"
     CDS             1307..>1577
                     /locus_tag="PKV30_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HRL31378.1"
                     /translation="MNTTPVSEYDDNEIVNDSPNEHMDAILAARLSRRGALKGGIGAT
                     TAALLGGVGLSACGGSDDDTPAPAPAPTPKSLNFAAVAKTNADTVS"
BASE COUNT          255 a          544 c          518 g          260 t
ORIGIN      
        1 gaaagaacac cggcaggccg caggccttga agatgtggtc gctctggtgc gcggcggtac
       61 tgctcttgct gatgtggtcg tcgcccgcca ccgcgatcac gccgccccac ggcgtggtgc
      121 cggccatgtt ggcgtgcttg aagacatcgg ccgtgcggtc aacgcccggc cccttgccgt
      181 accagatgcc gaacacgccg tcgaacttgt tgctgcccgg cggcgcaaag cccagttgct
      241 gcgtgcccca gatggcggtg gccgccagct cctcgttcac gccgggctga aagacgatgt
      301 gctgcgcctc caggtgtttg cgcgcgcccc acagcgcctg gtcgtagccg cccagcggcg
      361 agccgcggta gccgctgacg aagccggcgg tgttcttgcc gatcagcgca tcgcgctggc
      421 gctgcagcat cggcagcttg accagtgcct gcacgccgct catgaaggcg cggccgtagt
      481 ccaggctgta tttgtcgtcg agggtgacgg tttcgagcgc cttgcggatg tgctcgggca
      541 gcggggcatt catcgtgtct gtctcctgtg ggtgcgccct tgtgagccgg cgcagcgtcc
      601 cggccactgg gtgggtgcag ggcttttatg ggcaagcaaa gtgtatgccg cctgccgcga
      661 taagtgtttt ctttgcattc aacgccaagg cgtggtgcgg aaataatctt tcgtcaaaat
      721 agggatcatg gaaacacttg attcgttcga catcgccatc ctgcaacagc tgcaggccga
      781 cggccgcatg accaatgccg aacttgcgca gcgcgtgggg ctgtccgcgg ccccgtgctg
      841 gcgccgcgtg cgcgagctgg agcgcaacgg cttcatcacc ggctaccgcg ccgtcatcga
      901 ccgccacaag atcggcctgg gcgtgctggc cttcgtgcgg ctggacgccg cgcaaagcag
      961 cggcccgcag gtgcagaaga tggaagcggc catccgcgcc gtgcccgaca tcgtgagctg
     1021 ccactacatc agcggcagcg gcaccttcga gctgcaggtg gtcagcccgg acctcgacag
     1081 cttcagccgc ttcgcgcgcg aggtgctgct gcacctgccc aacgtgaagg acatgcacac
     1141 cagcttttcg ctcggcgagg tcaaggcggc cggcgcactg ccgctcgggc acatcgcgcg
     1201 gcgcccgcgc tgatggcacg gcgcgtcacg accttgtcac gcgcttgcca cattccccac
     1261 gcaccatccg cgctttgacc cgcaaccaga gcacggagtg cccacgatga acacgacccc
     1321 ggtttccgag tacgacgaca acgagatcgt caacgattcg cccaacgaac acatggacgc
     1381 catcctggcc gcgcgcctga gccggcgcgg cgcgctcaag ggcggcatcg gcgccaccac
     1441 ggcggccttg ctgggcggcg tgggcctgtc ggcctgcggc ggcagcgacg atgacacccc
     1501 ggcaccggcg cccgcgccaa cgcccaagtc gctgaacttc gccgccgtgg ccaagaccaa
     1561 cgccgacacc gtgagcg
//
LOCUS       DAOMVO010000462         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_22857_length_1576_cov_16.278107, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000462 DAOMVO010000000
VERSION     DAOMVO010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22857_length_1576_cov_16.278107"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKV30_14070"
     CDS             <1..651
                     /locus_tag="PKV30_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL31379.1"
                     /translation="RVIEREQTLMHERQRLMRDMHDGVGSSLMSALRLVEHGEAALVD
                     VPQVLRECIDDLKISIDSLEPVDADLLALLASLRFRLSPRLEGAGLALHWQVQDLPPL
                     PWLDAQSALNVLRVLQEVLTNIIKHSGAREITVSTAEGHQAHAPGVEVRIADDGGGFE
                     PPALDALPPGRKGLANIRARVHALGGRCRWQAGEGGGTVFVLWLPLEPGPASRAAA"
     gene            complement(716..>1576)
                     /locus_tag="PKV30_14075"
     CDS             complement(716..>1576)
                     /locus_tag="PKV30_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRL31380.1"
                     /translation="EGVTIRTGVLVGGMPGEGPGAKVTNLAKETVSAAQLTQDFDAVL
                     LTGGAEQSRDLPVPGRDLHGVHFAMELLPQQNRANAGDKVNDAISAKGKHVIVIGGGD
                     TGSDCVGTSNRHGAKSVTQFEVMPMPPEQENKPLVWPYWPIKLRTSSSHEEGAAREFA
                     VSTKEFVGDKGKVKALRTAQVEFKDGKLTEVPGTEKEWPADLVLLAMGFTNPVASVLE
                     AFGVTKDARGNAKATTDGAGCYATNVKKVFAAGDMRRGQSLVVWAIREGRQAARAVDE
                     FLMGSSSLPR"
BASE COUNT          214 a          539 c          541 g          282 t
ORIGIN      
        1 cgtgtgattg agcgcgagca gaccctgatg cacgagcgcc agcgcctgat gcgcgacatg
       61 cacgacggcg tgggctcgtc gttgatgagt gcgctgcggc tggtagagca tggcgaggcg
      121 gcgctggtgg acgtgccgca ggtgctgcgc gaatgcattg atgatctgaa gatttcgatc
      181 gattcgctgg agccggtgga cgccgacctg ctggcgctgc tggccagcct gcgctttcgg
      241 ctctcccccc ggctggaagg cgcgggcctg gcgctgcact ggcaggtgca agacctgccc
      301 ccgctgccct ggctggatgc gcaaagcgcg ctcaacgtgc tgcgcgtgct gcaggaggtg
      361 ctgaccaaca tcatcaagca cagcggcgca cgcgagatca cggtgtcaac agccgagggc
      421 caccaggcgc atgcgccggg cgtggaagtg cgcattgccg atgacggcgg tggtttcgag
      481 ccccctgccc tggatgcgct gccgcccggc cgcaagggcc tggcgaacat ccgcgcgcgt
      541 gttcacgcgc tgggtggtcg ctgccgttgg caggctggtg agggtggcgg gacggtgttc
      601 gtgctgtggc tgccgctgga gccgggtccc gcttcacgcg cggcggcgta gccggcgcgc
      661 gccatctgcc tgatagcgcc cgcgcggacc agccgaggaa gatggcgccc cagcctcagc
      721 gcggcaagct gctcgatccc atcaagaact catccaccgc acgcgccgcc tggcggcctt
      781 cgcggatcgc ccagaccacc agcgattgcc cacgccgcat gtcacccgcc gcgaacacct
      841 tcttcacgtt ggtcgcgtag cagccggcgc cgtcggtggt cgccttggcg ttgccgcgcg
      901 cgtcctttgt cacgccaaac gcttccagca cgctggcgac agggttggtg aagcccatgg
      961 ccagcagcac caggtcggcc ggccattctt tttcggtgcc gggcacctcg gtcagcttgc
     1021 cgtccttgaa ttcgacctgc gcggtgcgca gcgccttgac cttgcccttg tcgccgacga
     1081 attccttggt cgagacggca aattcgcgcg cggcgccctc ttcgtggctc gacgaggtgc
     1141 gcagcttgat cggccagtac ggccacacca gcggtttgtt ttcctgctcg ggcggcatgg
     1201 gcatgacctc gaactgcgtc acgctcttgg cgccgtggcg gttgctggtg cccacgcagt
     1261 cgctgccggt gtcgccaccg ccaatgacga tgacgtgctt gcccttggca ctgatcgcgt
     1321 cattcacctt gtcgcccgcg ttggcgcggt tttgctgggg cagcagttcc atcgcgaagt
     1381 gcacaccatg aaggtcgcgc cccggcacgg gcaggtcgcg cgactgctcg gcgccgccgg
     1441 tcagcagcac cgcgtcgaag tcctgcgtga gctgcgcggc cgacacggtc tctttcgcga
     1501 ggttggtcac cttggcgccc gggccttcgc cgggcatgcc gccgaccagc accccggtgc
     1561 ggatcgtcac gccctc
//
LOCUS       DAOMVO010000463         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_23099_length_1564_cov_14.826375, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000463 DAOMVO010000000
VERSION     DAOMVO010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23099_length_1564_cov_14.826375"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKV30_14080"
     CDS             complement(<1..194)
                     /locus_tag="PKV30_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31381.1"
                     /translation="MLCPEPNSTAVRRPRCRAALARPRPLAAALAVLLAACQSIEPPR
                     PETDPSQPPPAPITPPASSLP"
     gene            complement(633..1538)
                     /locus_tag="PKV30_14085"
     CDS             complement(633..1538)
                     /locus_tag="PKV30_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRL31382.1"
                     /translation="MSSKTKLAVLAAVGAAALLAGCETTNMKMGSAGSGDTVATGGAG
                     GATSSGENSQLEKCPSSLGTVSLVENENAGWYTILRNEYRLPPTANLLRLMIQQSNCF
                     IVVERGAAGMRAMSRERELMQSGQMRGGSNFGGGQMVASDYGLSPEIIFSEDNTGGGM
                     AALGGLIGGGRGRALGAIGGATKTREAAAMLTLVDNRSGVQVGASQGSASKTDFGGMA
                     GLFGVGGGAGLGGYQNTPQGKVISAAFMDAYNQMVRAVRNYRAQSVQGQGLGGGGRLG
                     VDGGAAPSQTYTPENAPRQPARRRR"
BASE COUNT          239 a          527 c          555 g          243 t
ORIGIN      
        1 ggcaggctgc tggccggcgg cgtgatgggc gcgggcggcg gctgcgacgg gtcggtctcg
       61 gggcgtggcg gctcgatcga ctggcaggcc gccagcagca cggcgagcgc cgccgccagc
      121 ggccgcggcc gcgcaagagc ggcgcgacag cgtgggcggc gaactgcggt tgagttgggt
      181 tcagggcaga gcatgcgctt gatcgtagcg cggcggcatg acctgcgcgt ggcggggcac
      241 gccactgcac gcgcgcaagc cggtggcgcc caagaatccg aatcgaatca ggcgccagcg
      301 cttgcctgat cagcgcgagc agctatcaaa attgcttgat tggcaagaag tcttagatgc
      361 tgtgtgggag cgcccttggc cgcgatggag ccgttgtgtg gcccaaggac gcgccatcgc
      421 gggcaagccc gctcccacaa ggctgatgac ggaacgcatg cgtcgtctga gacttcttgc
      481 caatcaagca aaattaaagc gaccgtgcca cacagccacg tccttggcgc cgctgagggc
      541 cacgcgccaa aacaaaggcc gctaaagcgg ccctggatat tcaaactgcc caatcccggc
      601 gctgacccaa gcggccagcg cccgggcgcc catcagcggc ggcgacgtgc cggctgacgt
      661 ggcgcgtttt caggcgtgta ggtctggctc ggcgcggcgc cgccatcgac gcccagacga
      721 ccaccgccgc ccaggccctg gccttgcacg ctttgcgcgc ggtagtttcg gaccgcgcgc
      781 accatctggt tgtaggcgtc catgaaggcg gcagagatga ccttgccctg cggcgtgttc
      841 tggtagccgc ccaggcccgc accgccgccg acaccaaaca ggccggccat gccgccgaag
      901 tcggtcttgg aagcgctgcc ttgcgaggcg ccgacttgca cacccgagcg gttgtccacc
      961 agcgtcagca tggcggcggc ttcgcgcgtc ttggtggcgc cgccgatggc gcccagggcg
     1021 cggccgcggc cgccgccgat caagccgccc agcgcggcca tgccgccgcc ggtgttgtct
     1081 tcgctgaaga tgatctcggg cgacaggccg tagtccgacg ccaccatctg gccgccgccg
     1141 aagttgctgc cgccgcgcat ctggccggat tgcatcagct cgcgctcgcg gctcatggcg
     1201 cgcatgccgg cggcgccgcg ctcgacgacg atgaagcagt tggactgctg gatcatcagg
     1261 cgcagcaggt tggcggtggg cggcaggcgg tattcgttgc gcaggatggt gtaccagccg
     1321 gcgttttcgt tctcgaccag cgacacggtg cccagcgacg aggggcattt ttcaagctgc
     1381 gagttctcgc cgctcgaggt ggcgccgccg gcgccgccgg tggccacggt gtcgccgctg
     1441 ccggcgctgc ccatcttcat gttggtggtt tcgcagccgg ccagcaaggc ggcggcgccg
     1501 acggcggcca gcacggccag tttcgtcttc gatgacatcc gatcaactcc agggttcagg
     1561 ccgc
//
LOCUS       DAOMVO010000464         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_23382_length_1549_cov_16.297858, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000464 DAOMVO010000000
VERSION     DAOMVO010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23382_length_1549_cov_16.297858"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..847)
                     /locus_tag="PKV30_14090"
     CDS             complement(86..847)
                     /locus_tag="PKV30_14090"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRL31383.1"
                     /translation="MKSAPRTGIVAAMHPELAAVLAAMPDERRQPVATRDFWCGHWAG
                     HEVVAVLSRVGKVAAATTATLLIERFKVDRIVFTGTAGGVGDGVRVGDVVVAGSFMQH
                     DMDASPLFPRFEVPLTGVARFDTDSDLSTGLARAAQTVLARAADTLGADAVAEFDLQA
                     PRVHRGLIASGDRFVATSAESAALRAALPEALAVEMEGAAVAQVCRDFNVPFAALRTI
                     SDRADDAAHVDFMRFIDQVASRYSAAILEAYFESL"
     gene            complement(924..>1549)
                     /locus_tag="PKV30_14095"
     CDS             complement(924..>1549)
                     /locus_tag="PKV30_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL31384.1"
                     /translation="PICEGYGLSETSPSATCNPVTSKEFTGTIGLPIPSTYIAILDDD
                     GREVPLGQPGEIAIKGPQVMAGYWQRPDETAKVMTEGGYFKSGDIGIMDERGYTKIVD
                     RKKDMILVSGFNVYPNEIEDVVAAMPGVLEVAAVGVPDEKMGEAVKLVIVKKDPGLTE
                     DAVRQFCHDNLTGYKRPRIIEFRGELPKTPVGKVLRRELRDKVATVN"
BASE COUNT          248 a          517 c          532 g          252 t
ORIGIN      
        1 gagggcagcc cctggtcact gggccagctc tttgaatgaa atcagcctcc agcgcttgat
       61 gggcaagcgc aagaagctct ctaaatcata gcgactcgaa atacgcctca agaatcgccg
      121 cgctgtagcg gctggcgacc tggtcgatga agcgcatgaa atcgacgtgc gcggcgtcgt
      181 cggcgcggtc gctgatggtg cgcagggcgg caaagggcac gttgaagtcg cggcagacct
      241 gggccacggc ggcgccttcc atctcgacgg ccagcgcctc gggcaaggcg gcgcgcagcg
      301 cggcgctttc ggcgctggtg gcgacaaagc ggtcgccgct ggcaatcagg ccgcggtgca
      361 cgcgcggcgc ctgcaagtcg aattcggcca cggcgtcggc gcccagcgtg tcggcggcgc
      421 gcgccagaac ggtttgcgcg gcacgggcca ggccggtcga cagatcggaa tcggtgtcga
      481 aacgcgccac gcccgtcagc ggcacctcga agcgcgggaa cagcggcgag gcgtccatgt
      541 cgtgctgcat gaagctgccc gccaccacca cgtcgcccac gcgcacgccg tcgcccacgc
      601 caccggcggt gccggtgaag acgatgcggt ccactttgaa gcgctcgatc agcaacgtgg
      661 cggtggtcgc ggccgccacc ttgccgacgc gcgagagcac cgccaccacc tcgtgcccgg
      721 cccagtggcc gcaccagaaa tcgcgcgtgg cgacgggctg gcgccgctcg tcgggcatgg
      781 ccgccagcac ggcggccagc tcggggtgca tggcggcaac gatgccggtg cggggagcgg
      841 atttcatgtc aaacagggcg ccagcgcttg tccatcaagc gcgagcagct ctcaaaacaa
      901 gagcgagcgt ggcacttgcg ccttcagttc accgtggcga ccttgtcgcg caactcgcgc
      961 cgcaacacct tgccgacggg cgtcttcggc agctcgccgc ggaactcgat gatgcgcggg
     1021 cgcttgtagc cggtcaggtt gtcgtggcag aactggcgca cggcgtcttc ggtcaggccc
     1081 gggtctttct tgacgataac cagcttgacg gcttcgccca tcttctcgtc cggcacgccg
     1141 acggccgcca cttcgagcac gccgggcatg gcggccacca cgtcctcgat ctcgttgggg
     1201 tagacgttga agccgctgac cagaatcatg tccttcttgc ggtcgacgat cttcgtgtag
     1261 ccgcgctcgt ccatgatgcc gatgtcgccc gacttgaagt agccgccttc cgtcatgacc
     1321 ttggcggtct cgtcggggcg ctgccagtag ccggccatga cctgcgggcc cttgatggcg
     1381 atctcgcccg gctggccaag cggcacttcg cggccgtcgt cgtccaggat ggcaatgtac
     1441 gtgctgggga tcggcaggcc gatggtgccg gtgaactctt tcgacgtcac cggattgcag
     1501 gtggccgagg ggctggtctc cgacaggccg tagccctcgc agatcgggc
//
LOCUS       DAOMVO010000465         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_23726_length_1531_cov_12.262873, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000465 DAOMVO010000000
VERSION     DAOMVO010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23726_length_1531_cov_12.262873"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..463)
                     /locus_tag="PKV30_14100"
     CDS             complement(110..463)
                     /locus_tag="PKV30_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31385.1"
                     /translation="MKTMHRILATGLLAAGALGLASTAQARNDVYWSVGVGGPGVSIG
                     FGNAPTYYPPAPVYYQPAPVYQPAPVYYQPAPVYVRPRPVYYGPPAYYYGPPGHRGHR
                     GHRHHGHGHGNGHWR"
     gene            711..>1531
                     /locus_tag="PKV30_14105"
     CDS             711..>1531
                     /locus_tag="PKV30_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRL31386.1"
                     /translation="MSSPAKPTPPAPAPASAADASAKPSNFIRHVIEQDLEKGTYASR
                     QWGGGPGDGPSHQAGAPDPAKVRMRFPPEPNGYLHIGHAKSIWLNFSLAKEYGGVCHL
                     RFDDTNPEKEEQEYVDSIRDTVRWLGWDHHFADDPARPGIKQPHEYFASDYFDFMYRA
                     AEYLIEAGHAYVDEQTPEQMRAHRGDFGTPGVNSPFRDRSPAENLKRFREMAHGQHAD
                     GAMVLRAKIDMASPNINLRDPAIYRIRHATHHNTGDKWSIYPMYTFAHPIEDALE"
BASE COUNT          313 a          558 c          451 g          209 t
ORIGIN      
        1 aaaagaaaag cccgctcggg tgtcgagcgg gctgcaacgg tggtcacctt ggagacgccg
       61 gcctgggcac tgcgggcccg ttttccggcg gaggagaacc tgaattcaat cagcgccagt
      121 ggccgttacc gtggccgtgc ccatggtgac ggtgaccacg gtgaccgcga tggcccggcg
      181 ggccgtagta ataagcaggc ggcccgtaat acaccgggcg cggccgcaca tacacgggtg
      241 ccggctggta atacaccggc gcgggctggt aaaccggcgc cggctggtaa taaaccggtg
      301 ccggcgggta atacgtgggc gcgttgccga agccaataga cacgcccggg ccgcccacgc
      361 cgaccgacca gtacacatcg ttgcgggctt gggccgtgct ggccagcccc aaggcgccag
      421 cggccagcag gccggtggcc agaattcggt gcatcgtctt catgatcatc actccttgaa
      481 actgagggcc cgccccttga acgacagaac gcggccgctg tgcgaattaa acgatccaac
      541 cgccgcccac gcgcacatca cttgcgtgaa acccgtatcc agccgtaaca cgcgccaatc
      601 aaatcaaggg tttacgtgag cctggccgac cggccatctg ccgccccacc gcgcgccgca
      661 gggccgctga tggggggttg acccccgggg ccgcgcccta gaatcggccc atgtcatccc
      721 ccgccaaacc cacgccgccc gcccccgcgc ccgcctccgc cgcagacgcc agcgccaagc
      781 ccagcaactt catccgccac gtcatcgagc aagacctcga aaaaggcacc tacgccagcc
      841 gccaatgggg cggcggccct ggcgacggcc ccagccatca agcgggagcg cccgacccgg
      901 ccaaggtgcg catgcgcttt ccgcccgagc ccaacggcta cctgcacatc ggccatgcca
      961 agagcatctg gctgaatttc agcctggcca aggaatacgg cggcgtgtgc cacctgcgct
     1021 ttgacgacac caaccccgaa aaagaagagc aggaatacgt cgacagcatc cgcgacaccg
     1081 tgcgctggct cggctgggac caccactttg ccgacgaccc ggcgcgcccc ggcatcaagc
     1141 agccgcacga atacttcgcc agcgactatt tcgacttcat gtaccgcgcg gccgaatatt
     1201 tgatcgaagc tggccacgcc tacgtcgacg agcaaacgcc cgagcagatg cgcgcgcacc
     1261 gcggcgactt cggcacgccc ggcgtcaaca gccccttccg cgaccgctcg ccggccgaga
     1321 atttgaagcg ttttcgcgag atggcgcatg gccagcatgc cgatggcgct atggtcttgc
     1381 gagcaaaaat cgacatggcc agccccaaca tcaacctgcg cgacccggcc atctaccgca
     1441 tccgccacgc cacgcaccac aacaccggcg acaagtggtc catctacccc atgtacacct
     1501 tcgcgcaccc catcgaggac gcgctggagc a
//
LOCUS       DAOMVO010000466         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_23788_length_1528_cov_19.272234, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000466 DAOMVO010000000
VERSION     DAOMVO010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23788_length_1528_cov_19.272234"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKV30_14110"
     CDS             complement(<1..298)
                     /locus_tag="PKV30_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRL31387.1"
                     /translation="MTYCCAIKINEGMVFLSDSRTNAGLDAISTFRKMLIYERAGDRF
                     MVMLSAGNLSISQSVREILQTEAIRDPESGDPITIWNAKSMFDAAQVLGAAVRHI"
     gene            complement(332..>1528)
                     /gene="mutS"
                     /locus_tag="PKV30_14115"
     CDS             complement(332..>1528)
                     /gene="mutS"
                     /locus_tag="PKV30_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRL31388.1"
                     /translation="NLRVQFNKVHGFYIEVTQGQASKVPDDYRRRQTLKNAERFITPE
                     LKAFEDKALTAQERALAREKWLYEHLLNALQPHVPQLTRYARAIAALDALCALTERSL
                     TLNWAAPQFVREPCIEIRGGRHPVVEARLAETSGAPFIANDTLLGLKSRMQIITGPNM
                     GGKSTYMRQVALIALLASVGSYVPAQSCRLGPLDAIHTRIGAADDLANAQSTFMLEMT
                     EAAQILHTATAESLVLMDEIGRGTSTFDGLALASGIAAHLHDKVGAFTLFATHYFELT
                     EFPASHRAAFNVHVGAAESGQGIVFLHELQPGPASRSHGIQVARLAGVPVAVVNHARH
                     VLGELEQGAHQSRAQVDLFAPPATTTPQEVSAVESALSVIDPDTLSPRDALDALYKLK
                     RLAAQD"
BASE COUNT          234 a          482 c          526 g          286 t
ORIGIN      
        1 tgatgtggcg caccgccgcg cccagcacct gggcggcgtc gaacatgctc ttggcgttcc
       61 agatcgtgat cgggtcgccg ctttcggggt cgcggatggc ctcggtctgc aagatttcgc
      121 gcaccgactg cgagatcgac aaattgcccg ccgacagcat gaccatgaag cggtcgcctg
      181 cgcgctcgta gatcagcatc ttgcggaagg tgctgatggc gtccaggccc gcgttggtgc
      241 gcgagtcgga caaaaagacc atgccttcgt tgatcttgat ggcgcagcaa taagtcatga
      301 tcactactaa attgagagct gcttgcgctt gtcagtcctg cgctgcgagc cttttcagct
      361 tgtaaagcgc gtccagcgca tcgcgtggac tcaaggtgtc ggggtcgatc accgaaaggg
      421 ccgattctac ggcgctcact tcctgcggcg tggtggtggc gggcggcgcg aacaaatcga
      481 cctgcgcgcg gctctggtgc gcgccttgtt ccagctcgcc cagcacatgc cgcgcgtggt
      541 tgaccacggc gactggcacg cccgccaacc gcgcgacctg aatgccgtgg ctgcggctgg
      601 cggggccggg ttgcagctcg tgcaaaaaga caatgccttg cccgctttcg gccgcgccca
      661 cgtgcacgtt gaaggccgcg cggtggctgg ccgggaactc ggtcaactcg aagtaatgcg
      721 tggcaaacag cgtgaaggcg cccaccttgt cgtgcaggtg cgccgcaatc ccgctggcca
      781 gcgccaggcc gtcgaaggtg ctggtgccgc ggccgatctc gtccatcagc accagcgatt
      841 cggcggtggc ggtgtgcagg atctgcgccg cctcggtcat ctccagcatg aaggtcgatt
      901 gcgcgttggc caggtcgtcg gccgcgccga tgcgggtgtg gatggcgtcc agcggcccca
      961 gccgacagct ttgcgccggc acataggagc cgacggacgc cagcagcgcg atcagcgcca
     1021 cctgccgcat gtaggtgctt ttgccgccca tgttggggcc ggtgatgatt tgcatgcggc
     1081 ttttaaggcc cagcagcgtg tcgttggcga tgaagggcgc gccgctggtc tcggccaggc
     1141 gcgcttcgac caccggatgg cggccgccgc gaatttcaat gcacggctcg cgcacgaact
     1201 gcggcgcggc ccagttcagt gtcagcgagc gctcggtgag cgcgcacagc gcatccagcg
     1261 cggcaatggc gcgcgcatac cgcgtgagct gcggcacgtg cggctgcagg gcgttcagca
     1321 ggtgctcgta cagccacttc tcgcgcgcca gcgcgcgttc ctgcgcggtc agggccttgt
     1381 cctcgaaggc cttgagttcg ggcgtgatga agcgctcggc gttcttcagc gtctggcggc
     1441 ggcggtagtc gtcgggcacc ttgctggcct ggccttgcgt gacctcgatg tagaagccgt
     1501 gcaccttgtt gaactgcacg cgcaggtt
//
LOCUS       DAOMVO010000467         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_23837_length_1525_cov_12.408163, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000467 DAOMVO010000000
VERSION     DAOMVO010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23837_length_1525_cov_12.408163"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKV30_14120"
     CDS             <1..486
                     /locus_tag="PKV30_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL31389.1"
                     /translation="PAEPPVEAAPAPAPPPAAPARKRSKTAAASPAPTAAAGLADPMQ
                     WWGALTQQFQHIAANAMRDASRAAATTAADAAANTPDSIAAGAGQTGAKASKRPAKKT
                     TAKRPAKKAAAKKRAAPAAKNAAKQTAKKTPTSKAAAPKDSVPKSALGWPLPPPFKLG
                     R"
     gene            513..>1525
                     /locus_tag="PKV30_14125"
     CDS             513..>1525
                     /locus_tag="PKV30_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRL31390.1"
                     /translation="MTTTLFPTGHATHPQWRMACALVLAQLRAQMALPGHASAPTLGL
                     LYITDAYAAAARDILDHLQAELPEVTDWAGTVGVGVCASNAEYIDEPALSVMLCDLPI
                     DQYRVFSGVAPLAAGLGFTPHTALVHADAQTPDVAELIAEMAQRTRTGYLFGGLAASR
                     GPVVQFAASGHGNAPGQGAAGGVFSGGLSGVAFGDGVALVSRVTQGAQPVAPEREVTA
                     ADGNVLITLDDEPALDLMLDDLAISLEQPRHALEALRRTLVGLSSPGERGVHRSGNFG
                     ADVLVRHIIGVDPGRRGVAIAQQPQVGQRVAFCQRNPQAARADLMRICAEIREELEPE
                     EVT"
BASE COUNT          240 a          611 c          505 g          169 t
ORIGIN      
        1 cccgccgagc cgccggtcga agccgcgccg gcccccgcgc cgccgcccgc agcccccgcg
       61 cgcaagcgca gcaagaccgc tgccgcctcg cccgcgccca cagccgccgc cggcctggcc
      121 gaccccatgc aatggtgggg cgcgctcacc cagcagtttc agcacatcgc cgccaacgcg
      181 atgcgcgacg cctcgcgcgc ggcagcgacc accgcggcag acgccgccgc aaatactcct
      241 gattcaatag ctgctggcgc aggtcagaca ggcgccaagg cctcaaaacg ccctgcaaaa
      301 aagaccaccg ccaaacgccc ggcgaagaag gccgcggcca agaaacgcgc cgcgcccgcc
      361 gccaagaacg cggccaagca gactgcaaag aagacgccga ccagcaaagc cgccgccccc
      421 aaggacagcg tgcccaaatc cgccctcggc tggccgctgc cgccgccctt caaactgggc
      481 cgctgacgcg gcgcggcggc gcacgccatg acatgacgac cacgctgttc cccaccggcc
      541 acgccaccca cccgcaatgg cgcatggcct gcgccctggt gctggcgcag ctgcgcgcgc
      601 aaatggcgct gcccggccac gccagcgcgc ccacgctcgg gctgctctac atcaccgacg
      661 cctacgccgc cgccgcgcgc gacatcctag accacctgca ggccgagctg cccgaagtca
      721 ccgactgggc cggcaccgtg ggcgtcggcg tgtgcgccag caatgccgaa tacatcgacg
      781 agccggcgct gtccgtcatg ctgtgcgacc tgcccatcga ccagtaccgc gtgttctccg
      841 gcgtcgcgcc gctggccgct ggcctcggct tcacgccgca caccgcgctg gtgcacgccg
      901 acgcgcaaac gcccgacgtg gccgagttga tcgccgagat ggcccagcgc acccgcaccg
      961 gctacctgtt cggcggcctg gcggccagcc gcggcccggt ggtgcagttt gccgccagcg
     1021 gtcacggcaa cgcgccgggg cagggcgcgg ccggcggcgt gttctcgggc ggcttgtccg
     1081 gcgtggcgtt tggcgacggc gtggcgctgg tctcgcgcgt cacgcaaggc gcgcaacccg
     1141 tggcgcccga gcgcgaggta acggccgccg acggcaacgt gctcatcacg ctggacgacg
     1201 agcccgcgct cgacctgatg ctggacgacc ttgccatcag cctggagcag ccgcgccacg
     1261 cgctcgaggc gttgcgccgc acgctggtgg gcctgtcgag ccccggcgag cgcggcgtgc
     1321 accgcagcgg caactttggc gccgacgtgc tggtgcgcca catcatcggc gtcgaccccg
     1381 gccggcgcgg cgtggcgatt gcgcagcagc cgcaggttgg ccagcgcgtc gccttctgcc
     1441 agcgcaatcc gcaggccgcg cgcgccgacc tgatgcgcat ctgcgccgaa atccgcgaag
     1501 agctggagcc cgaggaagtc accga
//
LOCUS       DAOMVO010000468         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_23965_length_1519_cov_15.230874, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000468 DAOMVO010000000
VERSION     DAOMVO010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23965_length_1519_cov_15.230874"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKV30_14130"
     CDS             <1..1154
                     /locus_tag="PKV30_14130"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRL31391.1"
                     /translation="KQSFGELARALDAGEVDLAVVRSNVMPRQGLTVAVLHELPVVFV
                     VVGKARLNSLADLRRHRVGVLRGTPENLELLDSLLRLAGIDPAQVTKVPLEPETLAAA
                     IDHKQVDAIMPLAPLQGAGRARLLALADRVGHSKLRVFGVDEGEALVESDPAMEAVEL
                     PRGALIGNPPLPAEGLPTLAVTQRLVARMQLPVSVVNDVTRLLFTSRMALAADVPSAK
                     SIEAPQTDTGEGLAAALPLHPGAAGYFSGDQNRFFDRYGDLVYVGAMALTGMFSGVGA
                     LISYLLARQRRNAARFTDQLLGLLGRARQAPDEPALQAVERDTDDLLALAFGRLSSGA
                     IGSEQFDTFATLNGSVQEAIARRARQLGHERAAARAEALAQRSSAPGSA"
     gene            complement(1212..>1519)
                     /locus_tag="PKV30_14135"
     CDS             complement(1212..>1519)
                     /locus_tag="PKV30_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL31392.1"
                     /translation="RTLNHAFVILDEAQNTTPEQMKMFLTRIGFGSKCVVTGDVSQID
                     LPKGATSGLIDAERVLKRVKGLAFTHFTSADVVRHPLVARIVDAYDAQGRPAARAAR"
BASE COUNT          209 a          502 c          575 g          233 t
ORIGIN      
        1 gcaagcagag ctttggcgag ttggcgcgcg cgctcgacgc cggcgaggtc gacctggccg
       61 tggtgcgcag caacgtgatg ccgcgccagg gcctgacggt ggcggtgctg cacgagctgc
      121 cggtcgtgtt tgtcgtcgtc ggcaaggcgc ggctgaattc gctggccgat ctgcgccgcc
      181 accgcgtggg cgtgctgcgc ggcacgccgg agaacttgga gctgctcgac agcctgctgc
      241 gcctggccgg catcgacccg gcgcaggtga ccaaggtgcc gctggagccg gagacgctgg
      301 ccgcggccat cgaccacaag caggtggacg cgatcatgcc gctggcgcct ttgcagggcg
      361 cgggccgggc gcggctgctg gcgctggccg accgcgtggg ccacagcaag ctgcgggtgt
      421 tcggcgttga tgagggcgag gcgctggtgg agagcgaccc ggcgatggaa gcggtcgagc
      481 tgccgcgcgg ggcgctgatc ggcaacccgc cgctgccggc cgaggggctg cccacgctgg
      541 ccgtcacgca gcggctggtg gcgcgcatgc agttgcccgt cagcgtggtc aatgacgtga
      601 cgcggctgct gttcaccagc cgcatggcgc tggctgccga tgtgccgtcg gccaagtcga
      661 tcgaggcgcc gcaaaccgat accggcgagg ggctggccgc cgcgctgccg ctgcacccgg
      721 gcgcggcggg ctatttctcg ggcgaccaga accgcttttt tgaccgctac ggcgacctgg
      781 tgtacgtggg cgccatggcg ctgacgggca tgttctcggg cgtgggtgcg ctgatcagct
      841 acctgctggc gcgccagcgg cgcaacgcgg cgcgctttac cgaccagttg ctgggtctgc
      901 tggggcgcgc gcggcaggcg cccgatgagc cggcgctgca ggcggtggag cgcgacaccg
      961 acgatctgct ggcgctggcc ttcggccgtc tgtcgagcgg cgccatcggc agcgagcagt
     1021 tcgacacgtt cgccacgctc aacggcagcg tgcaggaggc catcgcgcgg cgcgcgcggc
     1081 agttgggcca cgagcgcgcc gccgcgcggg ccgaggcgct ggcgcaacgg tcatcagcac
     1141 cgggttctgc ataaaaaaag gccccggcgc ttgtctgaca agcgcgaaca gctatcaaaa
     1201 caggagtatt atcagcgcgc agcgcgtgcg gccgggcgcc cttgcgcgtc gtaggcgtcc
     1261 acgatgcgcg ccaccagcgg gtgccgcacc acgtcggcgc tggtgaagtg cgtgaaggcc
     1321 agccccttca cgcgcttgag cacgcgttcg gcatcgatca gcccgctggt cgcgcccttg
     1381 ggcaggtcga tctggctcac gtcgcccgtc accacgcact tgctgccgaa gccgatgcgc
     1441 gtcaagaaca tcttcatctg ctcgggcgtg gtgttctgcg cctcgtcgag gatgacgaag
     1501 gcgtggttca gcgtgcgcc
//
LOCUS       DAOMVO010000469         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_24127_length_1511_cov_12.095467, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000469 DAOMVO010000000
VERSION     DAOMVO010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24127_length_1511_cov_12.095467"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKV30_14140"
     CDS             complement(<1..922)
                     /locus_tag="PKV30_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRL31393.1"
                     /translation="MIGPIRRTLTLIVLSVRDMLASAGPVVLLAVGVLVAAYWWLEPQ
                     PPKTVTLATGPDGSAYAEFGQRYAQALAKNHIQVKLLTTEGSLANLQALRAGDADVAF
                     VRGGSADPAADGEAGITSLGALFYEPLWVFYRPAALVKQAPAAPKSAASAPVSVTTLG
                     QLKGLRVNIDQPGSGVPEIVHRLLQANGMDASALVNSPLPPAAAAEALLGGYLDAMVL
                     VTAPESPVVERLLRAPGVALMDVAQADAYARRFPFLQPVTLPRGVVDLAADIPPHDVA
                     LLATTTSLLTREQTHPALRQLFAQAAQGLHS"
     gene            complement(952..>1511)
                     /locus_tag="PKV30_14145"
     CDS             complement(952..>1511)
                     /locus_tag="PKV30_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRL31394.1"
                     /translation="GAGATVGKLFGMGHAMKGGIGSAAVTLNGVTVGALIAVNALGDV
                     VDPATGAPLAGARTADGRALRGSVAAVLAGEAPLSLLAGVNTTIGVVATDAPLAKAQC
                     QRLAGAGHDGLARAIRPVHTMSDGDTLFALATGQAPALDFNVLCIMAAEAVARACVNA
                     VRAAEGIKTDTFDLPSASDLIAARA"
BASE COUNT          234 a          531 c          526 g          220 t
ORIGIN      
        1 cgctgtgcag cccctgcgcc gcctgcgcga acaactggcg cagcgcgggg tgcgtctgct
       61 cgcgcgtcag cagcgaggtg gtggtggcca gcagggcgac gtcgtgcggc ggaatgtcgg
      121 ccgccagatc gaccacgccg cgcggcagcg tgaccggctg caaaaaggga aagcgccgcg
      181 catacgcatc ggcctgcgcc acgtccatca gcgccacgcc gggcgcgcgc agcaggcgtt
      241 cgaccaccgg cgattcgggg gcggtgacca gcaccatggc atccaggtaa ccgcccagca
      301 gcgcctccgc cgcggcggcg ggcggcaagg ggctgttgac gagtgcgctg gcatccatgc
      361 cgttggcctg cagcaggcgg tgcacgatct cgggcacgcc gctgccgggc tggtcgatgt
      421 tgacgcgcag gcccttgagc tggcccagcg tggtcacgga cactggcgcg gatgctgcag
      481 atttcggagc tgctggcgct tgtttgacca gcgctgcggg ccgatagaac acccaaagcg
      541 gttcgtagaa tagggcgccg agcgaggtga tgccggcttc gccatcggcc gccggatcgg
      601 cgctgccgcc gcgcacgaag gccacgtcgg catcgcccgc gcgcagcgcc tgcaggttgg
      661 ccagcgagcc ttcggtggtc agcaacttga cctggatgtg gttcttggcc agcgcctgcg
      721 catagcgctg gccgaactcg gcataggcgc tgccgtcggg gccggtggcc agcgtcacgg
      781 tctttggggg ttgtggctcg agccaccagt aggcggccac cagcacgccc acggccagca
      841 agaccaccgg cccggccgaa gccagcatgt cgcgcaccga cagcacgatc agcgtcaggg
      901 tgcggcgaat cggtccgatc atgaatgaaa aacccttctg gcgcttgtcc atcaagcgcg
      961 agcagctatt aaatcactag ctgacggcaa gtcgaacgtg tccgtcttga tgccttcggc
     1021 cgcgcgcacc gcgttcacgc aggcgcgcgc cacggcttcg gcggccatga tgcacagcac
     1081 gttgaagtcg agcgctggcg cctggcccgt tgcgagggcg aacagcgtgt cgccatcgct
     1141 catcgtatgc accggccgaa tcgcgcgcgc cagaccatcg tggccggcac cggccagacg
     1201 ttggcattgc gctttggcca agggcgcgtc ggtggcgacc acgccgatgg tcgtgttgac
     1261 gcccgccagc agcgacagcg gcgcctctcc cgccagcacc gccgccacgc tgccgcgcaa
     1321 cgcgcggccg tcggcggtgc gcgcgccggc taggggcgcg cccgtggctg gatcgaccac
     1381 gtcgcccagc gcgttgacgg cgatcagcgc gcccaccgtt acgccattca acgtgactgc
     1441 cgcgctgccg atgcctccct tcatcgcatg ccccatgccg aacagcttgc ccaccgtggc
     1501 gccggcacct g
//
LOCUS       DAOMVO010000470         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_24144_length_1510_cov_14.635052, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000470 DAOMVO010000000
VERSION     DAOMVO010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24144_length_1510_cov_14.635052"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..699
                     /locus_tag="PKV30_14150"
     CDS             127..699
                     /locus_tag="PKV30_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRL31395.1"
                     /translation="MPDPRTRRQWLVTLAAGALLAGCASRRPVSEARGGMATPGAGAP
                     RRGPAPLPATQAPLALDPAGREASVAQAMLLVNTPYRYGGNTPEGGFDCSGLVHYVFG
                     QVAAGGARLPRSTAQWAAVTVPVDEGALQRGDLVFFNTGGALFSHMGIYVGGGQFVHA
                     PSTGGTVRKDSVSSRYFGPRYLGGRRVFAA"
     gene            complement(1268..>1510)
                     /locus_tag="PKV30_14155"
     CDS             complement(1268..>1510)
                     /locus_tag="PKV30_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRL31396.1"
                     /translation="AEPMNITVYTEGTGKISDDAIAKLVREHFDLRPKGIIQMLDLLR
                     PIYQKTAAYGHFGRDEPEFTWERTDKAAALRAAAGL"
BASE COUNT          216 a          469 c          548 g          277 t
ORIGIN      
        1 cgacgggggc gttggaaatc ggcggacacg cttggaaagt gcgtatgcta tacataaaat
       61 agctgctggc gcttgtccag caagcgcgag aggcctgaaa tgacctgttc ttcacccgct
      121 caaaccgtgc cggacccacg cacgcgccgc caatggctgg tcacgctggc cgcgggtgcg
      181 ctgctggccg gctgcgccag ccggcgtccg gtgtcggagg cgcggggcgg catggcaacg
      241 cctggcgctg gcgcgccgcg ccgtgggcca gcgccgctgc ctgccacgca agcgccgttg
      301 gcgctcgacc cggcggggcg cgaggcgagc gtggcgcagg ccatgctgtt ggtcaacacg
      361 ccgtaccgct acggcggtaa tacgcccgag ggcgggtttg attgcagcgg gctggtgcac
      421 tacgtgttcg ggcaggtggc ggccggtggc gcgcggctgc cgcgcagcac ggcgcagtgg
      481 gcggctgtca ccgtgccggt ggacgagggg gcgttgcagc gcggcgacct ggtgttcttc
      541 aataccggcg gcgcgctgtt ttcgcacatg gggatttatg tgggcggcgg gcagttcgtg
      601 cacgcgccgt cgacgggggg gacggtgcgc aaggacagcg tgtcgagtcg ctacttcggg
      661 ccacgctatc tgggggggcg gcgggtgttt gcggcttgag gtttttgggg ggttggaggc
      721 gctgctggcc gggggttcgc cccggcgggc gacctacttt tctttgctcc gccaaagaaa
      781 agtaggcaaa agaaaggcgg ccccactggc cgtggccctg cgggcagcct gcgatgctcg
      841 tgcgcggggc ggcgctgccg aactcgcttt gttcgcttcg ctctcgccgc tcaaacagcg
      901 gcagcgagtc agttcacaaa gcgcatgctt cgctgcgccg ccccacgccc tgcgcttctc
      961 ggcacggcca gaagggggtg aagtccattc gggccatcgc tgcgctcggc cttgggctga
     1021 tcagcgccgc tgcttcgcgc ggcggttttt gctccctctc cctctggggg aggcttggcc
     1081 tgtcctgagc ttgtcgaagg agggtgaggg caagccagtt cagacaggtg gaacgctgca
     1141 tgtcaccgca gcatatccgg tcttttcacc ccgcagcgca agcagatcaa gcgcgggctg
     1201 ctatcaaaaa ataacccgcc ctccttcgca ggtcgggcgg gttcgatgga cacagggcct
     1261 gaaaggctta cagccccgcc gccgccctca gtgccgcggc cttgtccgtg cgctcccacg
     1321 tgaactccgg ctcatcgcgc ccgaagtggc cgtaagcagc ggtcttctgg tagatcgggc
     1381 gcagcaaatc gagcatctgg atgatgcctt tcgggcgcag gtcgaagtgc tcgcgcacca
     1441 gcttggcgat ggcgtcgtcg ctgatcttgc cggtgccttc ggtgtacacg gtgatgttca
     1501 tcggctcggc
//
LOCUS       DAOMVO010000471         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ottowia sp. isolate AMR_MDS_5702
            NODE_24289_length_1503_cov_10.758287, whole genome shotgun
            sequence.
ACCESSION   DAOMVO010000471 DAOMVO010000000
VERSION     DAOMVO010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867923
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ottowia sp. (wastewater metagenome)
  ORGANISM  Ottowia sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ottowia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.103
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,834
            CDSs (total)                      :: 2,806
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 11 of 40
            Pseudo Genes (incomplete)         :: 24 of 40
            Pseudo Genes (internal stop)      :: 11 of 40
            Pseudo Genes (multiple problems)  :: 6 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ottowia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24289_length_1503_cov_10.758287"
                     /isolate="AMR_MDS_5702"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1898956"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKV30_14160"
     CDS             complement(<1..450)
                     /locus_tag="PKV30_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRL31397.1"
                     /translation="MYLRGRNIALPATLLALAVLAGCSSSANRAPVEDRGVTTAPRID
                     PTTLPGAENAGKPGFYTVRQGDTIMKIATEVNQPWRDIVRWNNLDNPNVIEVGQVLRV
                     VAPVGTTVAAAAPLTEAPRSTTPTPAPVPAKPPVPVATQTTPGAASAA"
     gene            complement(479..1285)
                     /locus_tag="PKV30_14165"
     CDS             complement(479..1285)
                     /locus_tag="PKV30_14165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRL31398.1"
                     /translation="MATPARPGFPARIDLGQGGKPPAKAPVRAPAAPGMPSPVTNASA
                     PQAASMARDPIPARARMVQLLAAEGISDAQVLRAMGAVDRHRFVDGALLAQAYEDTAL
                     PIGLGQTISKPSVVARMVELLMQGGLRGPDGKLGRVLDIGTGCGYQAVVLGQLASEVY
                     SIERLRDLHEKARANLRPLRIANVHLILGDGMAGFAKGAPYAGIIAAAGGEAVPDAWI
                     EQLAYGGRLVAPIATAAGQQALVVIDRSRQGIQQTILETVNFVPLKSGVA"
     gene            complement(1285..>1503)
                     /locus_tag="PKV30_14170"
     CDS             complement(1285..>1503)
                     /locus_tag="PKV30_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRL31399.1"
                     /translation="ITMDSPHGDTMYWIGGAGPASDATEGTDFHATAEGHIAITPLKV
                     DLTDRDALGYWAQGLGHMLAPHAKEGAG"
BASE COUNT          196 a          535 c          531 g          241 t
ORIGIN      
        1 ggccgcgctg gcggcgccgg gcgtggtctg cgtggccacc ggcaccggcg gcttggccgg
       61 cacgggcgcc ggagtcggcg ttgtgctgcg cggcgcctcg gtcaacggcg cggcggcggc
      121 cacggtggtg ccgaccggcg ccaccacgcg cagcacctgg ccgacttcaa tgacgttcgg
      181 gttgtccagg ttgttccagc gcacgatgtc gcgccacggc tggttgacct cggtcgcgat
      241 cttcatgatg gtgtcgccct ggcgcaccgt gtagaacccc ggtttgccgg cattttcggc
      301 gccgggcagc gtggtcgggt cgatgcgcgg ggccgtggtg acaccgcggt cttccaccgg
      361 cgcccggttg gcggacgacg agcaaccggc cagcaccgcc agcgccagca aggtggcggg
      421 cagcgcgata ttgcggcctc tcagatacat acagcgaacc cttttcagaa actgcttttc
      481 aagcaacccc tgattttaga ggcacgaagt tgacggtttc gaggatcgtc tgctgaattc
      541 cctggcgcga gcggtcgatg accaccagcg cctgctgccc cgccgccgtg gcgatgggcg
      601 ccaccagccg gccgccatag gccagttgct cgatccaggc gtccggcacc gcctcgccgc
      661 cggcggccgc gatgatgccg gcgtacggcg cgcccttggc aaaaccggcc atgccgtcgc
      721 ccagaatcag gtgcacgttg gcgatgcgca gcgggcgcag gttggcgcgc gctttttcgt
      781 gcaggtcgcg caggcgctcg atgctgtaga cctcgctggc cagctggccc agcaccaccg
      841 cttggtagcc gcagccggtg ccgatgtcga gcacgcggcc cagtttgccg tcgggcccgc
      901 gcaggccgcc ctgcatcagc agctcgacca tgcgcgccac cacgctcggc ttgctgatgg
      961 tctggcccag gccgatgggc agcgccgtgt cttcatacgc ctgcgccagc agcgcgccat
     1021 cgacaaagcg gtgccgatcc accgcgccca tggcgcgcag cacctgcgcg tcgctgatac
     1081 cctcggccgc cagcagttgc accatgcgcg cgcgcgccgg gatcgggtcg cgcgccatgc
     1141 tggcggcctg cggggccgac gcattggtca ccggtgacgg catgccgggc gcggccggcg
     1201 cacgcacggg cgccttggcc ggtggcttgc cgccctgccc caggtcgatg cgggccggaa
     1261 aacctggtcg cgccggcgtc gccatcagcc cgccccctct ttggcgtgcg gcgccagcat
     1321 gtggcccagg ccttgcgccc aataaccgag cgcgtcgcgg tcggtcaaat ccaccttcag
     1381 cggcgtgatg gcgatgtggc cttcggccgt ggcgtgaaag tcggtaccct cggtcgcgtc
     1441 cgacgcgggg ccggcgccgc cgatccagta catggtgtcg ccgtgcgggc tgtccatggt
     1501 gat
//
